LOCUS       DAWFLT010000001        95191 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWFLT010000001 DAWFLT010000000
VERSION     DAWFLT010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 95191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95191
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.001"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            52..1005
                     /locus_tag="WGR74_000001"
     CDS             52..1005
                     /locus_tag="WGR74_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ0904518.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1116..1439)
                     /locus_tag="WGR74_000002"
     CDS             complement(1116..1439)
                     /locus_tag="WGR74_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904519.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1612..2973)
                     /locus_tag="WGR74_000003"
     CDS             complement(1612..2973)
                     /locus_tag="WGR74_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ0904520.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAIRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSGKLGFELSEETTAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPAPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
     gene            complement(3270..4970)
                     /gene="recJ"
                     /locus_tag="WGR74_000004"
     CDS             complement(3270..4970)
                     /gene="recJ"
                     /locus_tag="WGR74_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ0904521.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAARRLADAVERQEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQELGLDVIVTD
                     HHLPADTVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLTALRAELRRRDYFSDGIKEP
                     NLGDLLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQMTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTFITDGSLPACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAA
                     "
     gene            complement(5039..5752)
                     /locus_tag="WGR74_000005"
     CDS             complement(5039..5752)
                     /locus_tag="WGR74_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904522.1"
                     /translation="MLFRKTTAAVLAATLMLNGCTFMLWGMNDPFSQTTDRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKQFQMVEPNPR
                     FAYQALPVKLESPASQNFSTEGLCLRYDTDRPADIAKLKQLEFEAVELDNRTIYTRCV
                     SAKGKYYATPQKLNADYHFEQSVPADIYYTVTKKHTDKSKLFENIAYTPTTLILDAVG
                     AVLALPVAALIAATNSSDK"
     gene            complement(5799..6311)
                     /locus_tag="WGR74_000006"
     CDS             complement(5799..6311)
                     /locus_tag="WGR74_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ0904523.1"
                     /translation="MKMFEQEDEWISKTQMKKQMNDLQDLGMALTKLSNDTLKKIGLD
                     ADLYEAVTAYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQR
                     VEQARVRLLADDGALTQFMSDFPHADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELK
                     TVMENGDAEI"
     gene            6458..7789
                     /gene="pmbA"
                     /locus_tag="WGR74_000007"
     CDS             6458..7789
                     /gene="pmbA"
                     /locus_tag="WGR74_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ0904524.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTISGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(8086..8289)
                     /locus_tag="WGR74_000008"
     CDS             complement(8086..8289)
                     /locus_tag="WGR74_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ0904525.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8532..8834
                     /gene="clpS"
                     /locus_tag="WGR74_000009"
     CDS             8532..8834
                     /gene="clpS"
                     /locus_tag="WGR74_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ0904526.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8836..11115
                     /gene="clpA"
                     /locus_tag="WGR74_000010"
     CDS             8836..11115
                     /gene="clpA"
                     /locus_tag="WGR74_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003746132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ0904527.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDDEDGNDSDGLGREG
                     EEAEQKTGSLSDYTVNLNTEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLVGEAG
                     VGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKSVLKQLE
                     KIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRTIFDK
                     DYALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSARYINE
                     RFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDDKQVL
                     QFLGRDLKNMVYGQENAIDALVAAVKMSRSGLGLPDKPIGSFLFSGPTGVGKTEAAKQ
                     LAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVLLLDE
                     IEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPGLGFTAKR
                     ERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIAKVVDKFLLRLEHRLLDKKVEAE
                     FTPALRKYLARKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSNGGFVRIDWDAAK
                     EEAVLKFKKSKVKPETETV"
     gene            complement(11162..13033)
                     /locus_tag="WGR74_000011"
     CDS             complement(11162..13033)
                     /locus_tag="WGR74_000011"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ0904528.1"
                     /translation="MPPLAIRRSAKNLKKKFKKFCQFGFTGTPIFPENALGAETTAGV
                     FGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKKLSAAENRQALLHPE
                     RIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLHPLK
                     VATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTDSKA
                     FQNYYRDLAKRVKNQEVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSRTNR
                     IYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYIDDRTGEVR
                     RGYLDVAKELRERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALRELQSV
                     DAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAVQDYRSAYNDIRDWLRRQKAGEQ
                     KEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRASIG
                     HRAKESLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRREAAELIAAEDLNETAAKR
                     YLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGTDI"
     gene            complement(12994..13365)
                     /locus_tag="WGR74_000012"
                     /pseudo
     CDS             complement(12994..13365)
                     /locus_tag="WGR74_000012"
                     /inference="COORDINATES: protein motif:HMM:NF037510.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(13384..14259)
                     /locus_tag="WGR74_000013"
     CDS             complement(13384..14259)
                     /locus_tag="WGR74_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0904529.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENQLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQIINQFEQTGTYANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLGVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSA"
     gene            complement(14338..14919)
                     /locus_tag="WGR74_000014"
     CDS             complement(14338..14919)
                     /locus_tag="WGR74_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ0904530.1"
                     /translation="MGEVFDLKNGYTPSKSNKEYWENGSIPWFRMEDIRENSRILDNS
                     LKHISKSAVKGGKLFPAKSIMMSTTATIGEHALIKVNYISNQQLTNFTIKDEFKDALD
                     INFAFYYFFIIAEQSKKLINTSSLPIISMKELKKLKIPIPPLPEQEKIAAILDKFDTL
                     THSISEGLPHEIALRRKQYEYYREQLLAFPKAT"
     gene            complement(14960..15559)
                     /locus_tag="WGR74_000015"
     CDS             complement(14960..15559)
                     /locus_tag="WGR74_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ0904531.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGGENGIAIIKTGQAVSKQKI
                     SNNIGSYPVINSGKEPLGYIDEWNTENDPIGITTRGAGVGSITWQEGRYFRGNLNYAV
                     TIKNRTELDVRFLYHILLEFEQEIHALCTFTGIPALNASNLKKLLIPIPPLETQQKIV
                     KILDKFTELEAELALRKRQYRYYRDFLLDFDNQIGGGDS"
     gene            complement(15725..16735)
                     /locus_tag="WGR74_000016"
     CDS             complement(15725..16735)
                     /locus_tag="WGR74_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEZ0904532.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTAAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(16732..18276)
                     /locus_tag="WGR74_000017"
     CDS             complement(16732..18276)
                     /locus_tag="WGR74_000017"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEZ0904533.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDYHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSINWIGSGDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEISETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(18349..19353)
                     /gene="rfaD"
                     /locus_tag="WGR74_000018"
     CDS             complement(18349..19353)
                     /gene="rfaD"
                     /locus_tag="WGR74_000018"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ0904534.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKERL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEEFIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKAGVERYVKWMLENLA
                     "
     gene            complement(19394..20512)
                     /locus_tag="WGR74_000019"
     CDS             complement(19394..20512)
                     /locus_tag="WGR74_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0904535.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPLIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEILLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKNKMRIYPEGREKYLEYHRKFVFQDNIDDFL"
     gene            complement(20514..21749)
                     /gene="dcm"
                     /locus_tag="WGR74_000020"
     CDS             complement(20514..21749)
                     /gene="dcm"
                     /locus_tag="WGR74_000020"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0904536.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(21786..22757)
                     /gene="hldA"
                     /locus_tag="WGR74_000021"
     CDS             complement(21786..22757)
                     /gene="hldA"
                     /locus_tag="WGR74_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ0904537.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(22812..23552)
                     /gene="pyrF"
                     /locus_tag="WGR74_000022"
     CDS             complement(22812..23552)
                     /gene="pyrF"
                     /locus_tag="WGR74_000022"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ0904538.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAASTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(24078..24725)
                     /gene="adk"
                     /locus_tag="WGR74_000023"
     CDS             complement(24078..24725)
                     /gene="adk"
                     /locus_tag="WGR74_000023"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ0904539.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            24940..25779
                     /gene="htpX"
                     /locus_tag="WGR74_000024"
     CDS             24940..25779
                     /gene="htpX"
                     /locus_tag="WGR74_000024"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ0904540.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSQGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(26080..26640)
                     /locus_tag="WGR74_000025"
     CDS             complement(26080..26640)
                     /locus_tag="WGR74_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904541.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGFTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(26846..27238)
                     /locus_tag="WGR74_000026"
     CDS             complement(26846..27238)
                     /locus_tag="WGR74_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0904542.1"
                     /translation="MAKSFNQAASELTDIFPNISLTDFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(27355..27765)
                     /locus_tag="WGR74_000027"
     CDS             complement(27355..27765)
                     /locus_tag="WGR74_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0904543.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(27779..28216)
                     /locus_tag="WGR74_000028"
     CDS             complement(27779..28216)
                     /locus_tag="WGR74_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0904544.1"
                     /translation="MKISIFWLYNAFILERSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(28628..29926)
                     /locus_tag="WGR74_000029"
     CDS             complement(28628..29926)
                     /locus_tag="WGR74_000029"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ0904545.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAESVKAEDVMAVIEKVAPRITPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVEACKPIYETMPGWSESTFGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(30029..31180)
                     /locus_tag="WGR74_000030"
     CDS             complement(30029..31180)
                     /locus_tag="WGR74_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0904546.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQSDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTETVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAIERQPAVLVDAE
                     DAEAAHEAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            31515..32894
                     /gene="norM"
                     /locus_tag="WGR74_000031"
     CDS             31515..32894
                     /gene="norM"
                     /locus_tag="WGR74_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ0904547.1"
                     /translation="MLLDLNRFSFSVFLKEIRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            32938..33978
                     /gene="murB"
                     /locus_tag="WGR74_000032"
     CDS             32938..33978
                     /gene="murB"
                     /locus_tag="WGR74_000032"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ0904548.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLRDIVRLPEFN
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHA
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            33990..34640
                     /locus_tag="WGR74_000033"
     CDS             33990..34640
                     /locus_tag="WGR74_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ0904549.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLAYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHREKYDRKIGNGNP"
     gene            34695..35471
                     /locus_tag="WGR74_000034"
     CDS             34695..35471
                     /locus_tag="WGR74_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0904550.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHSSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSSDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDKARECDETALPDPQTESVRQILAAEQHLLDSGISNIDILRLGG
                     LYCAERHPVSRLVQKQNILGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFAPDDSVGKIIRTVCDNGLSL"
     gene            35522..36088
                     /locus_tag="WGR74_000035"
     CDS             35522..36088
                     /locus_tag="WGR74_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ0904551.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVVRLNERLDRLERDIWID"
     gene            36108..36998
                     /locus_tag="WGR74_000036"
     CDS             36108..36998
                     /locus_tag="WGR74_000036"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ0904552.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            37023..37781
                     /locus_tag="WGR74_000037"
     CDS             37023..37781
                     /locus_tag="WGR74_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0904553.1"
                     /translation="MNPKTPPDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NHWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(37836..38501)
                     /locus_tag="WGR74_000038"
     CDS             complement(37836..38501)
                     /locus_tag="WGR74_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0904554.1"
                     /translation="MAVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(38615..39511)
                     /locus_tag="WGR74_000039"
     CDS             complement(38615..39511)
                     /locus_tag="WGR74_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ0904555.1"
                     /translation="MQLLKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTIARLTMTVYLPAEQKGTHMS
                     RFVALMEQYSEALDFAQLHRLTAEMVALLDSRAGKISVSFPFFRKKTAPVSGIQSLLD
                     YDVCLTGEMKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            39580..40737
                     /locus_tag="WGR74_000040"
     CDS             39580..40737
                     /locus_tag="WGR74_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ0904556.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKVLAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPLDCWLVLRGIKTLALRMNAHCQNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYELAKTQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHDVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(40800..41801)
                     /gene="hemB"
                     /locus_tag="WGR74_000041"
     CDS             complement(40800..41801)
                     /gene="hemB"
                     /locus_tag="WGR74_000041"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ0904557.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALNEVALDIREGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(41984..42268)
                     /gene="yhbY"
                     /locus_tag="WGR74_000042"
     CDS             complement(41984..42268)
                     /gene="yhbY"
                     /locus_tag="WGR74_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ0904558.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            42378..42998
                     /locus_tag="WGR74_000043"
     CDS             42378..42998
                     /locus_tag="WGR74_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ0904559.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            43062..45029
                     /gene="ftsH"
                     /locus_tag="WGR74_000044"
     CDS             43062..45029
                     /gene="ftsH"
                     /locus_tag="WGR74_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ0904560.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDKSVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            45251..45682
                     /locus_tag="WGR74_000045"
     CDS             45251..45682
                     /locus_tag="WGR74_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ0904561.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPNFLPWYSKTEVIGRNG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            45675..45953
                     /locus_tag="WGR74_000046"
     CDS             45675..45953
                     /locus_tag="WGR74_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ0904562.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            46006..46584
                     /gene="pth"
                     /locus_tag="WGR74_000047"
     CDS             46006..46584
                     /gene="pth"
                     /locus_tag="WGR74_000047"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ0904563.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARATLPDGDVWLLKPTTFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQARLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            46645..46851
                     /locus_tag="WGR74_000048"
     CDS             46645..46851
                     /locus_tag="WGR74_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ0904564.1"
                     /translation="MTELKQLIQTESILVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            46848..47210
                     /locus_tag="WGR74_000049"
     CDS             46848..47210
                     /locus_tag="WGR74_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ0904565.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            47441..48856
                     /locus_tag="WGR74_000050"
     CDS             47441..48856
                     /locus_tag="WGR74_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13/DASS family transporter"
                     /protein_id="HEZ0904566.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMIPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVALLRLSRGSMKAAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKERKTYVFVLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            48975..49484
                     /locus_tag="WGR74_000051"
     CDS             48975..49484
                     /locus_tag="WGR74_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0904567.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSAGAQFFINT
                     ADNAFLNHRSKEMYGRTVVQDWGYAVFGKVVGGFDVVDAIEGVSTKRHGYHDDVPTEP
                     VIITKAEAV"
     gene            49647..51029
                     /locus_tag="WGR74_000052"
     CDS             49647..51029
                     /locus_tag="WGR74_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ0904568.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGLTDSGIGVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLSIIEKDGFAAAGKQGSVTEKDISGEYHNHI
                     VGHVKLKRPMNIAIDAGNGVGGAFAGKLYRALRCGVTELFCDVDGNFPNHHPDPSKPK
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPGAK
                     VIFDVKSTRLLAPWIKEHGGEAIMEKTGHSFIKSTMKKTGALVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGRRVEFPDGFGLMRTSNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(51049..51804)
                     /locus_tag="WGR74_000053"
     CDS             complement(51049..51804)
                     /locus_tag="WGR74_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0904569.1"
                     /translation="MIKIRNIHKTFGKNTILRGINLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(51814..52530)
                     /locus_tag="WGR74_000054"
     CDS             complement(51814..52530)
                     /locus_tag="WGR74_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0904570.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(52520..53347)
                     /locus_tag="WGR74_000055"
     CDS             complement(52520..53347)
                     /locus_tag="WGR74_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0904571.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSESGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            53632..54540
                     /locus_tag="WGR74_000056"
     CDS             53632..54540
                     /locus_tag="WGR74_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ0904572.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKTRFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGHDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            54773..58387
                     /gene="recB"
                     /locus_tag="WGR74_000057"
     CDS             54773..58387
                     /gene="recB"
                     /locus_tag="WGR74_000057"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ0904573.1"
                     /translation="MSASIQAFDPLTVPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRGRLDDVLQVLESKGIAKLGDDTLSDGIAAYCAE
                     HHEGDTFLPELLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVEMTEEDGDRLLIPAQDFWRERVSNDPVLAALALKRKAVPQTVLAQISRYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTICRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHADT
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPERPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAARGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSSIFGYDAQQLHDFNQNESEILHWAESARTALDNWNKYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLGDQISLAENNSGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQGANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSTNTADNPLAYLIEGSPQ
                     DSRETVRRAYACEKDGIAMLKRNWRRVADNAPSGTNFAFTENAPPPAAYRSNAGQTAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYDLSDISPECRCPEMGFTLHTEDFSLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFVDMVCQDPDGNICVIDYKSNHLGTDASAYTQQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
     gene            58426..58785
                     /locus_tag="WGR74_000058"
     CDS             58426..58785
                     /locus_tag="WGR74_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ0904574.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAKAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            59027..59506
                     /locus_tag="WGR74_000059"
     CDS             59027..59506
                     /locus_tag="WGR74_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ0904575.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPSTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(59782..61161)
                     /gene="radA"
                     /locus_tag="WGR74_000060"
     CDS             complement(59782..61161)
                     /gene="radA"
                     /locus_tag="WGR74_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ0904576.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTDGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(61350..62414)
                     /locus_tag="WGR74_000061"
     CDS             complement(61350..62414)
                     /locus_tag="WGR74_000061"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ0904577.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEDDIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGSS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAEIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(62489..63706)
                     /locus_tag="WGR74_000062"
     CDS             complement(62489..63706)
                     /locus_tag="WGR74_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ0904578.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETH"
     gene            complement(63703..65037)
                     /locus_tag="WGR74_000063"
     CDS             complement(63703..65037)
                     /locus_tag="WGR74_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0904579.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKTIEAPSLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            complement(65052..65813)
                     /locus_tag="WGR74_000064"
     CDS             complement(65052..65813)
                     /locus_tag="WGR74_000064"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ0904580.1"
                     /translation="METVKPIMLIVRPSGRAKDDVETCRRAGWQAEVLSPIEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKMHISVGQGSRRVLERCLGRTV
                     IAPEDGNDSEAVLRLTVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            complement(65831..66253)
                     /locus_tag="WGR74_000065"
     CDS             complement(65831..66253)
                     /locus_tag="WGR74_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904581.1"
                     /translation="MKKMFLSAVLLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRATMKFQNGEFMYTVWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPQTAQANFNDADFSR"
     gene            complement(66504..66824)
                     /locus_tag="WGR74_000066"
     CDS             complement(66504..66824)
                     /locus_tag="WGR74_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ0904582.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            complement(66847..67473)
                     /gene="upp"
                     /locus_tag="WGR74_000067"
     CDS             complement(66847..67473)
                     /gene="upp"
                     /locus_tag="WGR74_000067"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ0904583.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(67579..67890)
                     /gene="grxD"
                     /locus_tag="WGR74_000068"
     CDS             complement(67579..67890)
                     /gene="grxD"
                     /locus_tag="WGR74_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ0904584.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(67991..68206)
                     /locus_tag="WGR74_000069"
     CDS             complement(67991..68206)
                     /locus_tag="WGR74_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEZ0904585.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            complement(68232..69011)
                     /locus_tag="WGR74_000070"
     CDS             complement(68232..69011)
                     /locus_tag="WGR74_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0904586.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVAAAASPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRTV"
     gene            complement(69016..69366)
                     /locus_tag="WGR74_000071"
     CDS             complement(69016..69366)
                     /locus_tag="WGR74_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ0904587.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            complement(69370..70347)
                     /locus_tag="WGR74_000072"
     CDS             complement(69370..70347)
                     /locus_tag="WGR74_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ0904588.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TVSGFRLRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            complement(70381..71493)
                     /locus_tag="WGR74_000073"
     CDS             complement(70381..71493)
                     /locus_tag="WGR74_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0904589.1"
                     /translation="MTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            71679..72380
                     /locus_tag="WGR74_000074"
     CDS             71679..72380
                     /locus_tag="WGR74_000074"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ0904590.1"
                     /translation="MSLKTVAVIGAMEQEIKLLREMMENIKAVSFGRFSAYEGELAGK
                     RMVLALSGIGKVNAAVATAWIIREFAADCVINTGSAGGLGKGLKVGDVVIGTETAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPKVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            72524..74317
                     /gene="lepA"
                     /locus_tag="WGR74_000075"
     CDS             72524..74317
                     /gene="lepA"
                     /locus_tag="WGR74_000075"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ0904591.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPAGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     IANPASEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKNGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            74460..75479
                     /gene="lepB"
                     /locus_tag="WGR74_000076"
     CDS             74460..75479
                     /gene="lepB"
                     /locus_tag="WGR74_000076"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ0904592.1"
                     /translation="MNIMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRNKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFHSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            75577..75683
                     /locus_tag="WGR74_000077"
                     /pseudo
     CDS             75577..75683
                     /locus_tag="WGR74_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(75744..76643)
                     /locus_tag="WGR74_000078"
     CDS             complement(75744..76643)
                     /locus_tag="WGR74_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0904593.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFA
                     SGLVSGLMLALSMVFVRKTHEIEPVALFPSMMILSLGGAVSLVVPALLMDGGVLYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQS"
     gene            complement(77209..78141)
                     /gene="cysK"
                     /locus_tag="WGR74_000079"
     CDS             complement(77209..78141)
                     /gene="cysK"
                     /locus_tag="WGR74_000079"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ0904594.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(78335..78940)
                     /locus_tag="WGR74_000080"
     CDS             complement(78335..78940)
                     /locus_tag="WGR74_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904595.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(78937..79788)
                     /gene="dapF"
                     /locus_tag="WGR74_000081"
     CDS             complement(78937..79788)
                     /gene="dapF"
                     /locus_tag="WGR74_000081"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ0904596.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVEQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPRFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIISRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            79925..80221
                     /locus_tag="WGR74_000082"
     CDS             79925..80221
                     /locus_tag="WGR74_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904597.1"
                     /translation="MFRILLLLFPCAILAACARNIPASPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            80235..80903
                     /locus_tag="WGR74_000083"
     CDS             80235..80903
                     /locus_tag="WGR74_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0904598.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(80976..83096)
                     /gene="pnp"
                     /locus_tag="WGR74_000084"
     CDS             complement(80976..83096)
                     /gene="pnp"
                     /locus_tag="WGR74_000084"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ0904599.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAINA
                     INEFADEVNPEVWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWSA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(83333..84196)
                     /locus_tag="WGR74_000085"
     CDS             complement(83333..84196)
                     /locus_tag="WGR74_000085"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ0904600.1"
                     /translation="MSEIGLVKIYFGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLVHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRAKAVRLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPTDQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            84395..85258
                     /gene="rfbD"
                     /locus_tag="WGR74_000086"
     CDS             84395..85258
                     /gene="rfbD"
                     /locus_tag="WGR74_000086"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ0904601.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGNAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFIR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYVGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
     gene            complement(85733..85966)
                     /locus_tag="WGR74_000087"
     CDS             complement(85733..85966)
                     /locus_tag="WGR74_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ0904602.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(86132..86332)
                     /locus_tag="WGR74_000088"
     CDS             complement(86132..86332)
                     /locus_tag="WGR74_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ0904603.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(86504..87379)
                     /gene="xerD"
                     /locus_tag="WGR74_000089"
     CDS             complement(86504..87379)
                     /gene="xerD"
                     /locus_tag="WGR74_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ0904604.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(87431..87871)
                     /locus_tag="WGR74_000090"
     CDS             complement(87431..87871)
                     /locus_tag="WGR74_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ0904605.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTLGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            88192..89130
                     /locus_tag="WGR74_000091"
     CDS             88192..89130
                     /locus_tag="WGR74_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ0904606.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            89259..89552
                     /gene="hfq"
                     /locus_tag="WGR74_000092"
     CDS             89259..89552
                     /gene="hfq"
                     /locus_tag="WGR74_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ0904607.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            89637..90203
                     /locus_tag="WGR74_000093"
     CDS             89637..90203
                     /locus_tag="WGR74_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0904608.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCHLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTHTETSIPALCATLSLLKEGGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLSYGFTNRKNNPPYLLAFEKLRQK"
     gene            90257..90631
                     /locus_tag="WGR74_000094"
     CDS             90257..90631
                     /locus_tag="WGR74_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ0904609.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRVFLLWKNPKKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            90641..91135
                     /gene="folK"
                     /locus_tag="WGR74_000095"
     CDS             90641..91135
                     /gene="folK"
                     /locus_tag="WGR74_000095"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ0904610.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLHQTSSLY
                     ATAPVGYDDQPDFVNAVCTVSTDMDGISLLSLLNRIEADFGRKRSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFVLGKHGKVAELSKQLGNQGIRL
                     LPDR"
     gene            complement(91263..91742)
                     /locus_tag="WGR74_000096"
     CDS             complement(91263..91742)
                     /locus_tag="WGR74_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ0904611.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDSVYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVLQI"
     gene            complement(91962..92699)
                     /gene="ubiE"
                     /locus_tag="WGR74_000097"
     CDS             complement(91962..92699)
                     /gene="ubiE"
                     /locus_tag="WGR74_000097"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ0904612.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(92742..93110)
                     /locus_tag="WGR74_000098"
     CDS             complement(92742..93110)
                     /locus_tag="WGR74_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ0904613.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQIADLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            93230..94849
                     /gene="mnmC"
                     /locus_tag="WGR74_000099"
     CDS             93230..94849
                     /gene="mnmC"
                     /locus_tag="WGR74_000099"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ0904614.1"
                     /translation="MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLNTPYAEPLCGLFWQHGVW
                     LNPPAFVRALLRHPLIGLHEDTPLTGISHDGEKWIASTPNGIFSATHIVYCTGAHSPC
                     LPEINLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALAESLFAANPNPQKYQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAVAAEILG
                     LPHPLSKRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(94846..94995)
                     /locus_tag="WGR74_000100"
     CDS             complement(94846..94995)
                     /locus_tag="WGR74_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904615.1"
                     /translation="MDSKGNLLGIPYSLFWEENVMGVFDILPSGLVCLFEYDFLWSGR
                     HAAKA"
BASE COUNT        22798 a        26723 c        23186 g        22484 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgt
      361 accaaacgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgcc
      481 gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggt tttctgcccg gtgacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt tgaccgtgtc
      661 accaagctga ttgaaaaagg cctgattgaa atcgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 ggcgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctttg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa atcgggcaga gaaaaacaga tacggaagcc aagctccgta
     1081 tccgtatccg tttctttatc cgtccaaccg atacatcaat cgaattttct gcatcggatt
     1141 gcctcatctt cgcaaaacat tgccgaagtt tcgcggttgc acgcctgacg cactttctgc
     1201 cgcgccacgc cttcattccg atcgcttgcc tcaaacgtct tctgaaaagg cgtaacgctg
     1261 catacataca tggtggaatt tgaaccgaaa aggtcgattt tgacggtatt gctgccgctt
     1321 tccaatacgg cgacacggtg ttccaactgc gccagacgtg cttccaaatc gccgattctg
     1381 tcggcaaatg caggcatggc ggcacacgcg gtcaaaatgc ccaaaataca ttttttcatc
     1441 aatttttcct tgtttcttga accctgccca cccggacatc ctttttccga gaggcagggt
     1501 tagactttat ttacggggat gaatcccctt acaaatcagg ataatacata gaacggcact
     1561 ttatgcttca tcccatgtgt tgaaacttca ttgctccttg tctgttgacc cttattccca
     1621 atccgttccc acaaccttcg gcttgggttt gcgcctgcga cggcggcgtt ttttcgcctg
     1681 tccttcgttt ttttcgtgtc gggcggcggc ttcgtttttg gtcatctccg accgctgttc
     1741 cgtcgatgcc gtctgaaacg ccgtccacca ctcggcaagg gcgcggtccg cattgccggt
     1801 ttcggcgcgc aagagcagga aatcataggc ggcacggaaa cgcgcctgtg caaacagttt
     1861 gtgcggacgt gcgcctttgc gattttcaaa ctgcggctgg aacatccaaa tttcgcgcat
     1921 cgtggcggaa aagcgttgcg gcacgcccca accgcgttcg acggtttcgc gcatcgtatt
     1981 gattgcatcg gacagggcgg gcgcgggttt caaaccctgt tgcagattgc ttttccaatg
     2041 gcgttccaac tcgggccaca tcagagccgc caatacaaaa ccgaccgaaa ccgatttgtc
     2101 ggcacgcagc cgctcatcgg tatttttcag ggcaagcacc gtcatttttc cggcgatgcc
     2161 gtctgaaaca cgcaaggcat tgagcagcgg atggatgtcg tccggtatgt caaatccgtt
     2221 caaacgtttc agacactcgc gcgcgtgccc tgaaaacagc agtttcatga tttcgtcaaa
     2281 cagcctcgct accggttcgt gcttcagacg gcatatcgat tcggcaatcg gtgcggtggt
     2341 ttcttccgac agctcaaagc ccaatttgcc cgacaggcgg atggcgcgca aaatcctgac
     2401 agggtcttcc tgatagcgtt cggcggcatc accaatcata accagcctgc gggcggcaac
     2461 atcggcaatc ccgttgtgga aatccaaaat ctcttctttt tcaggatcgt aatacaaggc
     2521 attgcaggta aaatcgcgcc gcatcgcatc ttcttcgatg ctgccgtagg tgttgtcttt
     2581 catgattctg ccgtgcgcgt tctgatgaac cttcgcaccg ccgcgaaacg tcgttacttc
     2641 gataatctcc gcaccgttca tcacatggac gatttgaaaa cgcctgccga tgatgcggct
     2701 gcggcggaag agtttgtgca cctgttcggg cgtggcatcg gttgccacat cgaaatcctt
     2761 gggttcgatg ccgagcagca gatccctgat cgcgccgccg accacataag cctgaaaccc
     2821 cgcccctttc agacggcgta tgacgttttc ggcggcaaag ctcaacattt cggcacggat
     2881 gttgtgtctt tcggcgggaa tgaccgtttt actttccgct tttttactgc tccgaccgga
     2941 aggcagcatc ttattcaacc attttttcag cataaatatt ctgacccgaa gagcggcatc
     3001 tccacaggca accgtgttca gacggcatca ggcgcggttt gcaagatgcc gtaaccataa
     3061 acaaaaggcg tagaaactac gccgtccgaa tcacgccgcc ctcgcggcgg caacgcgtca
     3121 ttataacaga ttgccctcca aaaaaatcag accctgcttt tgtggcggag tctgaaattt
     3181 gatgcacctt ctccgattgg gaacattccc gtcgtacaaa caggcaaatg aaggaacaga
     3241 aatcacattc aaacagtgtt ccgccgcctc tacgcggctt cccagtaatc gatatacagc
     3301 tgcaattcga gattgttgcg ccattcgttg gcaacggggc ggtaaaccgt gcggatgtat
     3361 tcgggaatgt cttcgctgca acgccaaaac atcgcttcaa attcgcagcc gtctttttgc
     3421 agccagactt ttttgtgttt gccctccgcg cccaaaggtt gctggcggac gacgtggaac
     3481 tcgtcggtaa agctcggcgg cgcgaagccc tgcccccaaa cgtgacgggc aaggttttgc
     3541 gcctgttcca acgtgatgtc gcaggcgggc aggctgccgt cggtgatgaa ggtttgcgac
     3601 aaatcgtctt cgcacaccat ttcgcgcacg gcttcttcaa aggccgtctg aaacgcggga
     3661 atgttgtgtt cgagtatgct caagcccgcc gccatcgcgt gtccgccgaa tttcaaaatc
     3721 aaatcgggat ggcgtttgga caccaagtcc aaagcatcgc gcaagtgaag attgggaatg
     3781 gaacgccccg aaccgcgcac ttcaccgttg tcggcaggcg caaacacgat ggtcggacga
     3841 taaaaacggt ctttgaggcg gctggcaacg atgccgacca cgccttgatg gaagtcgtcg
     3901 cgatacgcca ccaaagtcat ctgacctgaa ggcagggttt caggaaaagc attcagcgcg
     3961 tcttgcagca tagactgctc gatttcgcga cgctcgatat tgaggttgtt taactgagcc
     4021 gccagttcct gagcttcgga atcatctcgc gccaacaggc aggcgatgcc gaccgacata
     4081 tcgtccagcc gtccggcggc gttgatgcgc gggcccaacg caaaacccat atcaaacggc
     4141 tgcgccttgc gccaatcgcg ccgcgccact tcaaacaagg cgcggatacc ggggcgcatt
     4201 ttgccagagc gcatccgttt caaaccttgc gacacgagga tgcggttgtt gtggtcgaga
     4261 gggacgacat cggcaaccgt gccgagtgcg accaaatcca aaaggtcgcc cagattcggc
     4321 tctttgatgc cgtctgaaaa ataatcgcgg cggcgcaatt cggcacgcaa cgccgtcaac
     4381 acataaaaaa tcacgcccac gcccgccaag cttttgcttg gaaaaccaca gcctttttgg
     4441 ttcggattga cgatgatgca gtcgggcacg gtatcggcag gcaaatggtg gtcggttaca
     4501 atgacatcca aacccaattc ctgcgccctc gccacgcctg cgatgctggc gataccgtta
     4561 tcaaccgtaa tcagcaaatc cacgccttgc gcggcggcga tttcggccag ctcgggcgtt
     4621 aagccgtagc cgtgttcaaa gcggttgggc acgaggaaat ccactttcgc gcccatcgcc
     4681 gccaaaccgt ccaaaccgac ggcacacgcc gtcgcgccgt cggcatcata gtcggcgaca
     4741 atcaggattt tttcctgacg ctcaaccgca tccgccaaac ggcgggcggc ggcttcgcaa
     4801 ttcgtcagcg attgataagg caggagggaa gcgagtttgt cgtccaattc ggcaggactt
     4861 tgcacaccgc gcgaggcaca aagccgggcg attaaaggat cggcgccggc ggtgagcaaa
     4921 tgattgaaaa cgtcggtatt gacggatcgg gtttggattt tgacggacat agcgtggaac
     4981 tttacagtgc ggatgtgttt gaaatgccgt ctgaaggctt ttcagacggc attgccgttc
     5041 atttgtctga ggaattcgtg gctgcaatca acgccgcgac aggcaaggcc agcaccgcgc
     5101 ccaccgcatc cagtatcaac gtggtgggcg tatatgcaat attttcaaac aacttggatt
     5161 tgtcggtatg ttttttcgta accgtgtaat aaatatcggc aggcacactt tgctcaaaat
     5221 gataatcggc gttcagtttt tgcggtgtgg cgtagtattt gcctttggcg gagacgcagc
     5281 gcgtgtaaat ggtccgattg tcgagttcga ccgcttcaaa ctcaagctgt ttcagcttgg
     5341 cgatgtcggc aggtctgtcg gtatcgtagc gcaggcaaag gccttcggta ctgaaattct
     5401 ggctggcggg cgattcgagt ttgaccggca gggcttggta ggcaaagcgc gggttgggct
     5461 caaccatttg aaactgcttg tccaacccgg ccttcaaaat gcccgtcagc ttcgccgaat
     5521 cttcaggatt gacgacgaac cagtatttcc cgcccatcat caccaggctg cccttttcca
     5581 attgggcatt gtcttcggca accacaccga aggcgcggat ttggtccttg tcaacgtgtt
     5641 tgcggtcggt cgtttggctg aacgggtcgt tcatacccca caacataaac gtacagccat
     5701 tcagcatcaa ggttgccgcc aaaacggcgg cggtcgtttt acggaacaac attttatcct
     5761 ccaatctaaa taacggcgga tgtcgtctga aaatatgcct aaatttccgc gtccccgttt
     5821 tccataacgg ttttcaattc ttgaaacagg gcgcggaaat ttttcggcgg tttgttttgc
     5881 tcctgctctt ttttggtgtt gcggatcaag gtacgcaatt taccggcgtc cgcatgcgga
     5941 aaatccgaca taaactgcgt caacgcgccg tcgtctgcca acagccgtac gcgcgcctgt
     6001 tccacgcgtt gcaaaaaggc gttgtgcgcc gcatcgtcgc cgcgcagctt ggcaagaaac
     6061 gcctcgatgg gcgcgggatc cgtgtcgcgc atcagcctgc cgataaattg cgcctgccgc
     6121 ttgagcgcgc cgttggatgt gattttttta taggcggtta ccgcctcgta caaatccgca
     6181 tccaaaccga tttttttcag cgtatcgttt gagagcttgg tcaacgccat acccaaatcc
     6241 tgcaaatcgt tcatctgctt tttcatttgg gttttgctga tccattcgtc ttcttgttca
     6301 aacatcttga ttctcgaatt tttccaatag cgcattttaa cagaatgccg ccgcgttcag
     6361 acggcatttt gcaccgtccg ccctgcaaac ggcgcgcttt tgcccctgcc gcccgaaacg
     6421 gttaaaatgc cgcaaagcat catcaagaaa gatttctatg ctgttcaacc acaccgcttc
     6481 cgaactcctc gacctctgcc gccgcacgct cgacttggca aaagcagcgg gcgcgaccgc
     6541 cgccgaagcc gatttcagcg aatccatcgg acaaagcgtc agcgtgcggc tgggcgaaat
     6601 cgaacaaatc gagttccagc aggacaagtc gctggacatt accgtttacg tcggcaaacg
     6661 caaaggccgc gctggcaccg ccgatttctc cgaacaagcc ctgcgcgaca ccgtccgcgc
     6721 cgccgccgac atcgcccgcc acaccgccga agacggttgc gccggacttg ccgaccccga
     6781 gttgatggcg caacacatcg gcgaccccga cctttaccac gaatgggatt tggatacgga
     6841 agccgccgtc ggcttggcaa aacaatgcga acaagccgcc ctgaacgagg atgagcgcat
     6901 cgaaaactcc gaaggcgcgg cggtgcaaac cggccattac caatacgttt acggcaacac
     6961 ccacggtttt gccgcacacc ggcaaggcac gcaccacagc atttcctgca gcgtcgttgc
     7021 cgccgacgaa aacgggatgc agcgcgacta ctggtacgat tccgcctgcc gccatccgga
     7081 tatggacagc cccgaaacca tcggtcaaac cgccgcccgc cgcactttgc gccgactcgg
     7141 cagccgcagc atcccgacag gcagctaccc cgtcctcttc gataccacca tttcgggcgg
     7201 tcttatcgga cacctcgtcg gcgcactctc cggcggcgcg ctctaccgcc aaagcagttt
     7261 cctgatcgac agcatcggca aaaaagtcct gcccgatttc ctcaacctgc gcgaagaacc
     7321 gcacatcccc cgctctttcc gcagcagcta ttttgatgcg gaaggcgttg ccaccgcacc
     7381 gcgcttcgtg attcaaaacg gcattgtcga aggctatttc ctcagcagtt acagcgcgcg
     7441 caaactcggt atgcagacca cgggcaacgc cggcggcgcg cacaacctgt atttgaacca
     7501 tacgcacgaa acgcaatccg acctgctgaa agaaatgggg acgggattat tggttaccga
     7561 actgatggga cagggcgcga acaccattac cggtgactac tcgcgcggcg cggcgggttt
     7621 ttgggtggaa aacggcgtga ttgcctaccc cgtccacgag attaccgtag ccggacgctt
     7681 gcaggatatg taccgcgaca ttgtcggcgt ggcggatgac gctttgcggc gttcgtccaa
     7741 caaaatcggc tcgattctga ttgcggggat gacggttgcc ggaagctgaa cgccctgccg
     7801 atgccgtctg aagacgaagg cagcacgttt tgccccgtgc ctacccgttt gccgttttat
     7861 gccgcaccgc cttttgccga caacattgca cattttttca tcagcactga ttttccctta
     7921 tccgtcaaaa tactaagcca cttgtccaca acagggtaca cacaagttca ggataaggcg
     7981 tgcggggatt ggggtttttc cgccgtcggc aaaacggcag acaaaaacgg cgggacattg
     8041 cctgtcccgc cgttttcaaa tatacatttg accgtacatc aggaattaag cagcctgaat
     8101 gttggcggcc tgtttgcctt tagggccggt ggttacgtcg aaagagacgc gttggccttc
     8161 tttcagggtt ttgaaacctt ccatattgat tgctgagaag tgagcgaaca aatcttcgcc
     8221 gccttcatca ggagtgatga aaccaaaacc tttagcgtcg ttaaaccatt ttacgatacc
     8281 ggttgccatt agaaacttcc tatactcaaa aattaacaaa atcagcaaaa caaggcatac
     8341 ggcaaaccga gccgtctggc gttccttcca gcttgatata ctcggcggat gcccaatcct
     8401 gcttaaccgt ctttttacat ttgttaagac aaaacgtcaa gctatgtttt caaaaaagtg
     8461 ggaaaatagg caaaagtacc gaaaatacaa caaaaatgcc gtctgaacgc ggctttcaaa
     8521 tgggacacac tatgaccgcg caacaccaat ccgacacgct cttgcaccgt ctgaacaccc
     8581 tgccgccgaa acgttacggc gttttcctat tgaacgacga ttacaccacg atggaatttg
     8641 tcgtcgaaat cctgaccgaa atctttatgc tcggacaaga acaggcggta gcggtgatgc
     8701 tcttggttca tcacgaaggc aaaggcctgt gcggcactta cacgcgcgat attgcccaaa
     8761 ccaaacaaca acaagtcatg cagcgggcaa aagccgaagg gcatccgctg caatgtattg
     8821 tcgaggagat ttaatatgct tgcacccgaa ttggaacaga ttttgcagca gctttaccgc
     8881 gaggcgcgta aggctcatta tgaatttatc agcctcgagc atctgctttt ggtactcatc
     8941 gaagaagatg cctccgtgcc gaacgtctta aaactatgcg gcgcggattt gaaagtggtg
     9001 tccgaacagc tcgccgccag cgttgccgaa aacacccccc tgattcccga acacctttta
     9061 gacacggtcg aaacccggcc cacgctcggc ttccaacgcg tgatgcaacg ggcgatggtg
     9121 catacccagt ctgccggaaa agccgcagtc gaaccgttgg acgttttggt cgcgctgatg
     9181 agcgaaaccg acagccacac cgtctatttc ctcaagctgc aatcggttac gcgttttgaa
     9241 gttttgcgct gtattgccca tggctcgccc gatgacgagg atggcaatga ttcagacggc
     9301 ctgggacgcg agggcgaaga agcggaacaa aaaaccggct ccctttccga ctacaccgtc
     9361 aacctcaaca ccgaagtcaa agccggccgt atcgaccctt tgattggtcg caaacacgaa
     9421 atggaacggc tggtgcaaat cctatgccgc cgccgcaaaa acaatccgct tttggtcggc
     9481 gaagcaggcg tgggcaaaac cgcgctggcg gaaggtttgg cacatcaaat cgtcaacggc
     9541 ggcattccag acgcgcttaa agatgccgaa gtgtacgcgc tggatatggg ctcgctgttg
     9601 gcgggcacga aataccgtgg cgactttgaa gcgcgggtca aatccgtctt gaaacagctc
     9661 gaaaaaatcc cgcacgccat tttgtttatc gacgaaatcc acaccatcat cggcgcaggc
     9721 agcaccagcg gcggcaccat ggacgcgtcc aacctgctca aacccgcgct ggcaaaaggt
     9781 tcgctgcgct gcatcggcgc aaccacctac gacgaatacc gcaccatttt cgacaaagac
     9841 tatgccttaa gccgccgctt ccaaaaaatc gacgtggtcg aacccaccgt ttccgaaacc
     9901 gtacaaatcc tgcgcggctt gaagccgatg tttgaagcct tccaccaagt ccgctatacg
     9961 caaggcgcac tcgaagccgc cgccgaactc tccgcacgct acatcaacga gcgtttcctg
    10021 cccgacaaag ccatcgacgt gatggacgaa gcaggcgcgg cgcaacggat tctgcccaaa
    10081 tccaaacaga aaaaagtcat cggcaaagcg caaatcgaaa ccgtcatcgc caaagtcgcg
    10141 cggattccag aaaaaaccgt gtcgcacgac gacaaacagg tgctgcaatt cctcggccgc
    10201 gatttgaaaa acatggttta cggtcaagaa aacgccatcg acgcgctggt tgccgccgtc
    10261 aaaatgtcgc gttcgggctt gggactgccc gacaaaccga taggcagctt cctcttctcc
    10321 ggcccgaccg gcgtgggcaa aaccgaagcc gccaaacagc ttgcctactc gatgggcgta
    10381 ccgctgcaac gctttgatat gtccgaatac atggaacgcc atgccgtatc gcgcctcatc
    10441 ggcgcgcctc cgggctacgt cggcttcgaa caaggcggcc ttttgaccga agccgtcaac
    10501 aaacagccgc attgcgtgtt gctcttggac gaaatcgaaa aagcccaccc cgacattttc
    10561 aacgtcctcc tgcaagtcat ggacgcaggc aaactgaccg acaacaacgg caagagtgcc
    10621 gatttccgca acgtcatcct gattatgacc accaacgcgg gcgcggaaag cctcagccgc
    10681 cccggcctcg gctttaccgc caagcgcgaa cgcggcgacg aaatgcaggc catcaacaaa
    10741 ctcttcacgc ccgagttccg caaccgcttg gacgcgatta tcccgtttgc acccctgtcc
    10801 gaacccatta tcgccaaagt cgtggacaaa ttcctgctcc ggctcgaaca ccggctcctc
    10861 gacaagaaag tcgaagccga attcacaccg gcattgcgca aatatctggc gagaaaaggt
    10921 ttcgacccgc aaatgggcgc gcgcccgatg caccgcctga ttcaggaaaa aatccgcaaa
    10981 ccgctcgccg acgaactcct gttcggcaaa ctatccaacg gcggcttcgt gcggatagac
    11041 tgggatgcgg caaaagaaga agccgtgttg aaatttaaga aaagcaaggt taaacctgaa
    11101 acagaaactg tttaaccgat tgaaaaataa aatgccgtct gaaatttcag acggcatttt
    11161 tttaaatatc tgtccctatt ccggcaaact tttccacaaa cgccgcaatc ttttgaaaaa
    11221 cactttgttt cttcgtcaga tattgcgggt tgagcgggct cattttcggc agggtttcgg
    11281 tcagttccgt gccgttttcg ctggcatagc cgcgtttgag cgagccggtc aaataacgtt
    11341 tggcggcggt ttcgttcagg tcttcggcgg caatcagctc tgctgcttcg cgcctcatca
    11401 cctcttgagc gtaggtgtaa aaggtttcca gtatggcagg aacgtcgggg actttgtcca
    11461 aatccgtatc gttgatgaaa tccacaatca ggctctcttt ggcgcggtgg ccgatgctgg
    11521 cgcggatgat gcggcggatt tcttccacca gctccgcttt gcctttgatt tttttgtggt
    11581 gctcgaaaac cagttgcaag atgtaatcca gattgatttc ctgcgatttg agcaaatcca
    11641 cctcaaaaac cacatcgtcc caatcgattt ttgattgttc tttctgttcg cctgcttttt
    11701 ggcggcgcag ccagtcgcgg atgtcgttat aggcagaacg gtagtcctgc actgcccttt
    11761 cagacggcat cggcacttgc cgcatttcct gcacgtcttc atcgctcagg tagtattttt
    11821 cttgaaacgc cttcatcgca tcttcgtccg ccgcgtccac actctgcaac tcgcgcagcg
    11881 cggcaaattc atcgtagttc tgcaatacgt tttccgcccg caggtattcg ccgaagagtt
    11941 tggcaaaatc ttttttgtct ttttccgttt cgattttgtc gggatcgggg aaacgctcgc
    12001 gcaattcttt tgccacatcc aaataaccgc gccgcacttc gcccgtccga tcatcgatat
    12061 agccgttcat gtattcttcg tagctttttt ccaacaccac gtttttggtg tttttatcgc
    12121 cgaacagggt aatcgcatca atggttgcct gctccaaatc gcggaagcag acaatattgc
    12181 cgaaggtttt ggtggcatcg taaatgcggt tggtgcgcga aaacgcctgc atcaggccgt
    12241 gatagcgcag gtttttatcg acaaacagcg tgttcagcgt cggcgcgtca aaacccgtca
    12301 aaaacatacc gaccacaatc agcaaatcca cttcctggtt tttcacccgt tttgccaaat
    12361 cgcggtagta gttctgaaag gctttgctgt ccgtgccgaa attggttttg aaacaggcgt
    12421 tgtaatcgtt gatggcagcc tgcaaaaatt ctttcgcgct gctgtccatc gcttccggtt
    12481 caaaggtctc atcaacaatt tcaccgacgg cgttctgctc ttcgttggcc gcaaaggaaa
    12541 aaatggtggc cactttcagc gggtgcaggc tgcctgcctg ttgtgttttg aacgcttcgt
    12601 aatagcactt cgccgcatcc acgctgctga ccgcaaacat ggcgttgaag cctttaccgc
    12661 ccgcattcag gcggtgcgtt ttctgcctga actggttcag gatatattgc gtgatttcgc
    12721 ggatgcgttc ggggtgcagc agggcttggc ggttttcggc agcactcagt ttcttctcat
    12781 cctgttccgc ttccacggct ttgaactgcg ggcgcacatc gttgtaatcc actttgaatt
    12841 tcaatacttt ttcatcgcgg atggcatcgg taatcacata agaatgcaac tcacgcccga
    12901 acacgcccgc cgtggtttcc gcgcccaaag cgttttcggg aaaaatcggc gtaccggtaa
    12961 aaccgaactg gcagaatttc ttaaatttct ttttcaggtt ttttgcgctt cgccgaattg
    13021 cgagcggtgg cattcgtcga aaataaatac aacctgctga tggtaaaccg gcagattatc
    13081 ttcactcttc atcaggttgt tcagcttttg gatggtggta acgatgattt tgttgtcgtc
    13141 tttttccaaa ttgcgtttca agcccgccgt gctttccgaa ccattcacgc tgtcgggcga
    13201 aaaacgttgg tattccttca tcgtctgata gtccaaatcc ttcctgtcca ccacgaagaa
    13261 aaccttgtcg ataaacgccg attccgtcgc cagacgcgcc gccttaaagc tggtcagcgt
    13321 tttgccgctg cccgtggtat gccaaacata gccgccgctt tccggtttgc tccaattctt
    13381 tacctacgcc gagctgttga ttttccacaa aatgcgttcg gcggcggcaa tctgatacgg
    13441 ccgcataatc agcagcgtat cattcgcatc gaacacgctg taatgcagca atacgcccag
    13501 caatacgctt ttctgcagga acgtggcggt aaagtctttc aaatccttaa tcggatgatt
    13561 gtccgaccgc gcccaattca tcgtgaaatc aaagctgttt ttgtcgcgct tggtggtgtt
    13621 ggcgaaatag cgcgtgtccg tgccgttgga aatcacgaag atttgcagga atttgaacag
    13681 cgaattttcg ctgttgaagc tctctttgct gtaacggtgc acctgattga atgcttcgcg
    13741 caccgccacg ccgcgctttt tcaattcaat ctgcaccagc ggcaggccgt ttaccagcac
    13801 ggtaacgtca tagcggtttg catacgtgcc cgtctgctca aactgattga taatctgcac
    13861 atggttgcgg gcaaggtttt tcttgtccag cagataaatg tttttcagac ggccgtcatc
    13921 aaaagcgaaa tcataaatat ggtcgtcatg gattttgcgg gttttatcgg taatgttttc
    13981 agacggcctg tccagatatt ccgtcaaaaa ccgcgcccat tcgccgtctg aaaacgccac
    14041 atcgttcagc cgctgcaact gcgcgcgcag gttttccagc agcctgcttt ggctgttcaa
    14101 atccttgcgg tattcgtaac cctgattctg caaatcggcg attaactccg cttccaactg
    14161 gttttccgat tggtagctgc ccgactgttc gattttttca tattggtcga gcacgatgaa
    14221 attcggcgtt tcggcgatgg gtttggtttc gaggttcatg ggtcgtcctt tgcagtatcg
    14281 ggttttaaac ggtttgatgt tcggattggg ctgcctgaaa atgtgcaggc tgctttttca
    14341 ggtagcctta gggaaggcaa gcagctgttc gcggtaatat tcgtattgtt tgcggcgcag
    14401 ggcgatttcg tgcggcaggc cttcgctgat agagtgggtc agggtatcga atttgtctag
    14461 gatggcggca attttttcct gttcggggag tggtgggatg gggattttta atttctttaa
    14521 ttctttcatg ctaataatag gtaaacttga agtattaata agttttttgc tctgctccgc
    14581 aataatgaaa aaataataga aagcgaaatt tatatctaaa gcatccttaa attcatcttt
    14641 aattgtaaaa ttggtcagtt gttggttact aatataatta acttttatta acgcatgctc
    14701 tcctatggtt gccgttgtcg acatcattat tgatttagca ggaaacagct tgccgccctt
    14761 aactgcgctt tttgatatat gcttcaatga attatctaaa atacggctat tttccctaat
    14821 atcttccatt ctaaaccaag ggattgagcc attttcccaa tattctttat ttgatttgga
    14881 aggggtatag ccattcttca agtcaaacac ctcccccaac gtcttccaaa ccacatcttt
    14941 cagacggcct ttatagccat cagctatccc cccccccgat ttgattgtca aaatctaaaa
    15001 gaaagtcgcg gtaataccgg tattggcgtt tgcgcagggc taattcagct tccagctcgg
    15061 tgaatttgtc aagtattttt acaatttttt gttgggtttc caggggtggg atagggatta
    15121 ataatttttt caagttactg gcatttagag caggaatacc tgtaaatgta cataaggcat
    15181 gaatctcttg ttcaaattca agtaatatat gatagagaaa acgaacatcc aattctgttc
    15241 tattcttaat agtaactgca taatttaagt tgccacgaaa atatcttcct tcttgccacg
    15301 ttattgagcc gactcccgct cctcttgttg tgatgccgat agggtcattt tcagtgttcc
    15361 attcatcaat atagccaagc ggctctttgc cactgttaat aactggataa ctaccaatat
    15421 tatttgagat tttctgctta cttacagctt gtcctgtttt tatgatagct atcccatttt
    15481 ctccacccaa cggcttccac tccaccggtg ccgtctgaat catctcaatc aattttttcg
    15541 ctttgctttg catatccatg gttgttttcc gtttctttcc cgctgtggcg ggatgtgggg
    15601 cggttgtggg gtgcttcggc tcactctgtc cgtttgccgt tatttctgcg ggctgaagtc
    15661 cgtcctgctt tcaggctgcc tgcaatggtg tgcaggctgc tttttcaggt agcctgaatg
    15721 gtagttattt tgtgcggtct tttttctgtt tgatctgttg cacggctgtt ttttccaacg
    15781 ctttaacgct ttccagatag gcttgctcca ccggcgacag gatgcgcgct tcataggcgc
    15841 ggtattcgcg ttcggctttc tgctctgcct gtttgcggct gatgctgcct gcaccctgta
    15901 atgtgccttg tccgtacagc ccggaaaatt tgtccaattc ggctatccag tcgcgcatat
    15961 acatggggct ttgctcctgc gctttgattt ccgccaggtc gaagaaggcg gaaaccagac
    16021 ggttcaggcg gaacagttcg tcttcggtca gatagttttt ggcgattttg gcttcattca
    16081 gcgtggggat tgcgccttga aaggtggtca gccccataaa gtctttgctg ctgtcggcac
    16141 ggctgtatat cagctcagct gcggtttgcc ggctggcggc atagtgcagt ttgttttgaa
    16201 cggcggcaaa aaaggtttgg ctttcgctgc ttttggggtt gtagtcttgg ctggtggcat
    16261 ataaatcaag cacttgccgg tatagggctt tttcgctgct gcggatgtcg cgaatgcggt
    16321 tgagcagttc tttccaatag tcgccgccgc ctgtgccttt caggcgttcg tcgtctatgg
    16381 caaagccttt ggtcagatat tcgtccagcc gttcggttgc ccattggcgg aattggatgc
    16441 cccgcgcgga acggacgcgg tagccgacgg caataatcat gggcagggaa taatgggcga
    16501 ttttgcggtt taccttgcgc ccgttttcat tttgaactgt caactggaag ttgacagttg
    16561 ccttctcttc caattcttgc tctgcaagaa tggttttaat gtgtttgctg atattttgtt
    16621 tggtggtttg gtacagttct gccatgtccg cctgcgtcag ccaaagctgt ccgccaaatt
    16681 cctgcaaggc aaattgggca gtgccgtcgt tggcggtgta taggatgatg ctcatgcttc
    16741 aatctctgca atcacttcgt caatttcacg ccgcagccgt tcgattttgg caacggtttc
    16801 gctgatttcg gcgttaagct gtttgatgtc gataatttcg cgggtgtctt cgggttcaac
    16861 atagctgctg acggcgaggt tgtagccgtt gtctttgacg gtttgctggg cggcgttttg
    16921 ggcgatatgc ggcacatcgg ctttatcggc gaagagtttg acgatttcgg caatgtgttc
    16981 ttcggttaag acgttgttgt tggtttcttt tttaaagaag ccgcctgcgt cgatgaattg
    17041 gatgtcggta ttgtctttgt gtttggacaa aaccagtata ttgacggcga tgccggtgcc
    17101 gtaaaagaga ttgggcgcaa gggcgatgac ggtttccacg tagttgccct ccaccagata
    17161 ttggcggatt ttctgctctg cgccgccgcg atagaaaatg ccggggaatg agacgatggc
    17221 ggcgcggcct ctgccggaaa ggtagttcag tgcgtgcaga atgaaggcaa aatcggcttt
    17281 ggatttcggg gcgagtacgc ctgcaggggc aaagcggtcg tcgttgatta aggtggggtc
    17341 gccgctgcct atccagttga tggaataggg cggattggaa acgacggcat caaagggttt
    17401 gctgtctttg agtttgggat tggtcagtgt gtcgcccaat tcgatgtgga atttgttgta
    17461 attgacgttg tgcagaaaca tattcatgcg ggcgaggttg taggtggtgt ggttgatttc
    17521 ctgcccgaag aagccttctt cgatgatgtg ctcgtcaaac tgttttttcg cctgcaagag
    17581 caggctgccc gagccgcaag ctgggtcgta gattttgttt actttctcct gcccgtgcac
    17641 cgccagccgc gcaatcagct tggatacgct ttgcggggtg aaaaattcgc cgccggattt
    17701 gcctgcgttg gcagcgtagt tggaaatcag gtattcgtag gcatcgccga aaaggtcgat
    17761 gtggtagtct tcaaaactgc cgaaatcgag ttccgccacg ccttttagga cggcggcaag
    17821 gcgtttgttc ttgtcggcaa cggtgctgcc gagccggctg ctggtggtgt cgaagtcgtc
    17881 aaacaggcct ttaatgtctt gttcggacgg atagccggag gcggagcttt caatcgcggt
    17941 aaaaatttct ttcagcttgg tgttgagctc ttcgttttga tgggcttcgg cggcaatatt
    18001 gcaaaaaagc tggccggggt agatgaaata gcctttgact ttgacggcat cgtctttgat
    18061 ttcgggcgtg atgatgctgt ccggcatagc ggcgtaatca atactgctgt cgcctgcctg
    18121 catatagtcg gtaaagtttt cgctgataaa gcggtagaaa agtgtgccga gaacgtattg
    18181 tttaaagtcc cagccgtcca ccgcgccgcg tacttcgtcg gcaattttcc aaatttggcg
    18241 gtgcagttgg gcgcgttgtt gcatttcggt catcatcgaa atccatataa aagttaaaca
    18301 aatcgaaatc gcctgatatt ttcagacgat tttttacggg cattcaaatt aagccaaatt
    18361 ttccagcatc catttgacgt agcgctcgac gcctgctttg acatcgaaaa attcttccgt
    18421 ataacctgcc tcgcgcaatt tggtgatgtc ggcttgggtg aagctttggt atttgccttt
    18481 gagcgcgtcg ggaaatggga tgtagcggat aaattcttct tctaccaact cttttaaact
    18541 catttcaggt ttgccttcgg cggcgcggca ggcgttgacg gtggcagcgg cgagttcgtt
    18601 aaactgttgg ctgcgacctg ttcctaggtt gtagatgccg gaaagttcgg gatggtcgaa
    18661 gaagtagagg ttgactttgg cgacgtcttc gacgctgacg aagtcgcggg tttgttcgcc
    18721 gttgccgtag ccatcgttgc tgccgaacag gttgacgtaa ccgtgttcgc ggtattggtg
    18781 gaaatggtgg aaggcgacgg atgccatgcg acctttgtgt tgttcgtgtt gtccgtaaac
    18841 attgaagtag cggaagccga cgacttgggc ggtcagcctt tctttcatgc ggcggcgcaa
    18901 tacttggtca aacaggaatt tggaatagcc gtacacatta agcggttttt ccagttcgcg
    18961 ctcttcgcgg aagatttcgc ctttgccgta aaccgccgca ctggaggcat aaaggaaggg
    19021 aatgcgttcg tcctgacacc agtccaacaa atccagcgtg tactggtagt tgttttccat
    19081 catatacaaa ccgtcgtgat tcatggtgtc ggaacacgcg ccttgatgga aaacggcttc
    19141 gatgttttga taaggtaaaa tgtgttccct cacttggcgg atgaattcat gtttgtcgag
    19201 ataatgggcg atttcgcact cggcaaggtt tttgaatttt tcgcctttgc tcaaattatc
    19261 gacggcaaca atgtcagtaa taccgcgttg attaagtgct ttgacgatgt tgctgccgat
    19321 aaagccggcc gcgcctgtta cgatgatggt catattcggt ttcctttgtt ttgagtaaaa
    19381 ttaataaact tagttacaag aaatcatcga tattgtcctg aaacacaaat ttgcgatgat
    19441 attccaaata tttttctctt ccttcgggat atattctcat cttatttttt gcatttggat
    19501 tgatattaag tttttcccat gtgtaacggc ttagtcgagt accgcaaatc aaacgaccat
    19561 catccaagaa agtaatataa ccttgatcga acaaccaatc ataagtcggc gttaaagcta
    19621 acccatttaa ataatctaat gcctctttct catttttctc agtaatacaa accatataag
    19681 gtttaatatg actagccctt aacaaaatct catctgtaat taatgtaaat ggacattgcg
    19741 gcatatgatt tataacatcc ttgcgatatt tctgctgccc ttttctactt ttctgtttaa
    19801 tgatttcttg ctctacgaat tgattaaatt ctgaagccgg tatttctaat ttttctcttg
    19861 ataagagttg cgggtgcacg atagagcgaa attgataatc caaaaatatt cggaaataaa
    19921 ataatggttc tgaatcttct atatctttga caggcaatag tttaagaatt gataaataac
    19981 taattttggg caatacaatc ttacgccaaa ttgaccatat atcatcctcc gaacggatat
    20041 aacccctacg gtaaccttta gaattttcca aatggtctga tacatcatgg attgaaaaga
    20101 aaatattttc tgtaggcaga ttattaatac ttaaaacgta atcatcataa aaccttgaaa
    20161 tatcatgatg atatttctgt tcttgtttgt gatattcaac ttttgcatca accaaataac
    20221 gctgtaaatt tttcttatta aaaaaacaat ttttctgaat gagaggatat tctaccctcc
    20281 ttccattttt tgtctcagta atcccccaat tttcatactc aaaaaatgta cttagttttc
    20341 gaccactatc atcaatataa ctacctacat atttgcgtgc ttccccatta ccaccaaaaa
    20401 cagataactt gttattcctg tgaataaaac tatcttccgc acgtaaattt tgaatactat
    20461 cgacaataaa atactctatg ttttcaatag taattctttg aaaatccggc attttactct
    20521 ccataaagcg taatatccat ttgttttaat atagcgatta atacatcaac aacaatactg
    20581 tttcccgctt ggcgatacat ttgagtatca ctaactaaaa ttttaaaatc atctctaaaa
    20641 cccattagcc gcaaacattc cctaggtgtt aatttacgaa tacgtccacg attatgagta
    20701 acataattat caaccccggc acgatgcatt ttatgcatag tttgcaataa tgggcgagct
    20761 acaggcaaat cagtttccgt actggtttta aaattttttg ttcctcctgc caaaacataa
    20821 tttttgattt tttcagaaag ataatatttt tcctcaacgt tatttacatc aaaaataaag
    20881 tcatcaaatt cagattcgcg ggctgcctga aaaataaaat caccatgcca attaaattgt
    20941 tgatttgctt tttggcataa ggcaatttct ccattaatct gtgtataacg tttttgtcta
    21001 tttttagaac tggtaacaaa tttcacaccc ttttcacgta aaaaatattt gctatcagta
    21061 tagtcctcca aaaaatcttg catagtatgt tctaattcaa tcttttcagg aaactgaaaa
    21121 ccatttatag gaggggtatg aaagccaaca acaaaaatac gctcacgatg ttgaggaatc
    21181 ccataatcct tactattcat tatttggaaa tataagtcat aaccaagtga ataaaaaaca
    21241 ctttttacaa ctttccaagt ttttccatta tcatgattga gcaagccctt tacattttca
    21301 taaataaata tctttggttg gacttcatcc acaacgcgag caaattcata gaataatgtt
    21361 cctcgtgtat cttctaatcc tgcacgtttg ccaaccatgg aaaatgcttg gcaaggactg
    21421 cctccaacta aaatatcaac ttgatttcta aactttctcg catcaaattg agtaatgtcg
    21481 ttatgccaaa aatcttcatt taatttatag tttccaagat aacttttttt aacgtatgga
    21541 tcaatatctc ctgcaaaaac aatggtatgg tttaaattta atcggtggaa tgcctgttca
    21601 accgcaccaa tcccgctgaa tacggttgct aatctaatat gtgaatcagg tttaagaaaa
    21661 gttttagatt tccaaccttg ttgactggga aagagcaaag ttttttgtaa tcgagtatcg
    21721 tgtgtctgtg ccattgtcga aatagtcata cttatatcgt tctgtttatc ttatcaatat
    21781 gaaaactaca ttgttgattg ccctgacaat gccttgatca attcggcaaa cgagcaaacc
    21841 gccgtaccga gtttcgccac gacaaccccg gccgcagtat tggcaaggta catggcttcg
    21901 ggcatggtgc agcctgccgc caaacccaag cccattccgg caatgacggt gtcgcccgca
    21961 ccggatacgt cgtaaacttc ttgggcgcgg gtgggctggt aaatcggttc gccttcgctg
    22021 aacaaggtca tgccttcttc gcttcgggtc agtaaaacgg cggtcaggtc gaggtggcgg
    22081 cgcaggtttt gcgctttttc ggtcagctcg ctttcgtttt tccaactgcc gaccacttct
    22141 ttcaattcgg cgcggttagg cgtaatcaga gttgcaccga catatttttc gtaatcgtcg
    22201 cctttggggt cgattaagac ggttttgccg gcgtgtttcg cccaatcgat catatcggag
    22261 atatgcgaca ggccgccttt gccgtagtct gaaaaaatga ttgcgtcgta ttcgggcaag
    22321 atttcgcggt atttctgctt gatttgttcc aacacttcgc agttgggatg ttcttcaaaa
    22381 tcaagacgga taagctgctg gttgcgggcg acgacgcgca gtttgacggt ggtggcgatt
    22441 tgtttgtcgc gcatcagata ggaggcgacg ccgtcctgca ccatcagcgc atcgagcgcg
    22501 tcggcggctt cgtcgttgcc ggttacggac aacagccctg ccctgccgcc caacgaagcg
    22561 atgttgcgcg cgacatttgc cgctccgccc gcgcgttggt cgattcgtcc gattttcgcc
    22621 accggcacgg gggcttcggg cgaaatacgg gacacatcgc cgaaccaata gcggtcgagc
    22681 atcacgtcgc cgacaaccag gactttggct tgcgcgaaac gggatttgag ggtttcttgt
    22741 tggaacttgg cggacatatt gcctccgttg tattttttca gacggcctca gcctgtaagg
    22801 ccgtctgaaa atcagtttgc ttcaaggttt gccacgcggt tcacttcgcg caatacggct
    22861 accggatcgg cagcttgggt tacaggacga cccattacca aataagtcga agcggcagcc
    22921 aaggcttcgg ccggtgtcat gatgcggcgc tggtcatcat tattgccggc aacgtccaag
    22981 cggatgccgg gcgtgaccaa gacaaaatct tgtcccaact cgcggcgcag cggtgcggct
    23041 tcttgggcgg aacaaaccac gccgtccaag cccgaacttt gcgccagttt tgccaagcgg
    23101 atgacttgtt cttcaggggc ggtgttcaaa ccgatttccg ccaaatcgct ttgttccatg
    23161 ctggtcaaca cggttacgcc gattaagagc ggctttgtac cgtatccggc aacggcttct
    23221 gctgcggctt ccatcatacg gcggccgccc gatgcgtgca tatcgaccat ccaaacgccc
    23281 atatcggcag cgactttgca ggcttgcgcg acggtgtggg gaatatcgtg gtatttcaaa
    23341 tcgaggaaaa gtttgaaacc ttgattgatt aagctttctg ccaaattgcg tcccgtcgcg
    23401 gtaaacagct ctttgccgat tttgatttga cacaatgtcg ggtcaaggtt gcggacgaat
    23461 ccgagcgtgt ctttttcgtt ggaaaaatca agggcgacga tgacgggggt gcgttgttgc
    23521 ggagtttgga agtcggagat taaaggattc atggtgtttt cgtttcgggt tttcagacga
    23581 cctttatcgt ctttttcgca gtatagtgga ttaactttaa accggtacgg cgttgtctcg
    23641 ccttagttca aagagaacgc ttctctaagg tgctgaagca caaagtgaat cggttccgta
    23701 ctatctgtac tgcctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactataaca
    23761 acgtaaattc aaaccgtagg tcgggcattg atgtccgacc tacgcggact caagcctccg
    23821 acaaactgtt tcggataaat ttgaaccggt acatcagccg tcattcccgc gcaggcggga
    23881 atccattgat aaaactcagt tcatcagatt taaacagcga ttgcccaaat tcagaaatgg
    23941 attcccgcct gcgcgggaat gacgccaaat gattattttt cggttgcaac acgctgttta
    24001 tcaaaaacag gtcgtctgaa aacctgattt tctgttttca gagtgcgaag cctgcccgtg
    24061 ggtacgacct tttcgattta tttgcccaat gcgcccaata cttcggcttt cacggcttct
    24121 accggctgag tgccgtcaac tttgatgtat ttaggcgcgt gttcgccttc cagtttgctg
    24181 taaaaatcga ccaaaacttc ggtttgctcg tggtaaacgg caaggcgttt tttcacggtt
    24241 tcttctttgt cgtcgtcgcg ctgaatcaaa tcttcgccgg ttacgtcgtc tttgccttca
    24301 actttgggcg ggttgtaggt aacgtggtaa gtacggccgg aagccaaatg cacgcggcgg
    24361 ccgctcatac ggtcgacaat cacgctgtca ggcacgtcga tttcaacgac tgcatccaaa
    24421 tccacgcctg cttcaaccat cgcttcggct tgtgccaatg tgcgcgggaa accgtcgaac
    24481 aggaaaccgt ttttgcagtc gtcttgcgcg atgcgttctt tgaccatgcc gataatgatg
    24541 tcgtcgcgca ccaagccgcc ttcgtcaatg atttttttcg cttccaaacc caagggcgtg
    24601 ccagccttaa tcgcggcgcg gagcatgtcg ccggtagaga tttgcggaat gccgaacgcg
    24661 gcggtgatga attgcgcctg agtgcctttg cccgcgcccg gcgcgcctaa aagtaatgct
    24721 ttcatgaagt gatcctttga aatgtgttta ttggaatcaa acagatggtt tacgattcta
    24781 cacggcttgg cacaatagtt caaaaaccaa ctgcacaaac tgccatttgc aagcaaatgt
    24841 tttgcatacc tttgccgagt ctgatattca caagccggca actttttggg acaatcccca
    24901 aaatttcaaa acatcacacc ctaccaaaaa ggaacatccg tgaaacgaat ttttctgttt
    24961 ttggctacca atatcgctgt tttggtcgta atcaacattg ttttggcggt tttgggcatc
    25021 aacagccagg gcggcacggg cagcctgttg gcgtattccg ccgtcgtcgg cttcactggt
    25081 tcgattattt cgctgctgat gtccaaattc atcgccaaac aatcggtcgg cgcggaagtt
    25141 atcgacacgc cgcgcaccga agaagaagcc tggcttttga acactgtcga agcccaagcg
    25201 cggcaatgga acctgaaaac gcccgaagtc gccatctacc actcccccga acccaatgcc
    25261 tttgccacgg gcgcatcgag aaacagctcc ctgatcgccg tcagcaccgg tttgctcgac
    25321 catatgacgc gtgacgaagt ggaagccgta ttggcgcacg aaatggcaca cgtcggcaac
    25381 ggcgatatgg ttacgctgac gctgattcaa ggcgtggtca atacctttgt cgtgttcctg
    25441 tcgcgcatta ttgccaacct gattgcccga aacaacgacg gcagccagtc ccagggaact
    25501 tatttcctgg tcagcatggt attccaaatc ctgttcggct tccttgccag cttaattgtc
    25561 atgtggttca gccgacaacg cgaataccgc gccgacgcgg gcgcggcaaa actggtcggc
    25621 gcgccgaaaa tgatttccgc cctgcaaagg cttaaaggca acccggtcga tttgcccgaa
    25681 gaaatgaacg caatgggcat cgccggagat acgcgcgact ccctgctcag cacccaccct
    25741 tcgctggaca accgaatcgc ccgcctcaaa tcgctttaaa ccgatttgaa acggcaaaaa
    25801 ccgtacgcgc aagcagtacg gttttttgta tatgctgtct gaaaacatca ggtttccata
    25861 ccgacaccaa ccgtatccat ttccgcaccg gatacgccgc attgatatac cctgataaac
    25921 cgtatccgga cagaaaatat agtggattaa caaaaaccag tacggcgttg cctcgcctta
    25981 gctcaaagaa aacgattctc taaggtgctg aagcaccaag tgaatcggtt ccgtactatt
    26041 tatactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccccta tatcagataa
    26101 aacagcggta aaattgccgt ctgaaaccaa ctctgcgcca tacgggacac gctctgcacg
    26161 tctatttgcc tccgatatat cctccctact gtaggaacgt ctgccgattt gcacgaaatc
    26221 ggcacctcta gcacccttca ggatggcgga cgttatccgt tcttgggatt ctgcaagacg
    26281 gtcgacggca ttatacagac gctcggtctg atacccttcc gaagcaacct gcttcaggta
    26341 ttccaaataa ccgtaaccca acgcccctcc gacaagcagg gtcgtcaacg ctaccagcgt
    26401 tgccgaacgg attttagggg gcatatcttg aagcatatct ctaccgaacc gaaccaaagt
    26461 ctctttaaag tcggactcta cacgcgtaaa accctcggaa atcttgaagt tcaactcata
    26521 ctgcttccgt ttgtccgctg tcaaccgctt gaggttgtca tccccgtatt ggacggttga
    26581 cacaaccctg tacagactgt cctgtatttc gaccagcgtc tgtgcaaagt tggcggtcat
    26641 aactggattc ccaacccttg ccgccgattc tgatactcaa aacggtatcc aggcgttcca
    26701 gcagggcttc gggagacagc agtgcgtatt cttcaatatt ttccataggt tgattatagc
    26761 agaaccttat acctgttaca gacaaggttg tcgggatttg aacgtccgtc gagcttggtt
    26821 cgtaggattt agggtgttgt gaaccttacc tatcgtaccc aagcactttg tacctgtttg
    26881 aaataaggtt gtcgggcgac gttcgaccct tgagcctgtg attgatttgt tggaaatgta
    26941 cacctaaagc agcacctgcg gcagtaacgc ttgggaacgt gagtgtttcg ttggtcgttg
    27001 tgtcgaggat gaggaggggc ttccctacat ttggaggtgt ttgggttttt gacatcttag
    27061 cacactcagg acacccgtac tttgacttta taagggcatt aaatgtcgaa taacgaacgt
    27121 tcccgtgcat aggacaatta acggttactg ggtaattcac accgtcaaag tcggttagag
    27181 agatattagg gaatatatca gtaagttcag aagctgcttg gttgaaagat ttggccattt
    27241 gagactccaa aattgattgt caaatggatt taatagcaaa tttataaaaa tgtcaaattt
    27301 ttcgagaagc acctgatagt ggcgaggggt atacactgtt aaaaagtgta tacgtcattt
    27361 agtgcagatg tgaaccttat acctccctgc aagtaaagtc tctgtgtgaa cccgaccttt
    27421 tatacggtca gtgatagctg ctgggtttat acccaatgac cttgcagcag atgctcttga
    27481 ggggaatgta agtgtttcgc cagttacagt atcctccaat actgtatttt tagataattt
    27541 atctattgta tcttctggaa ttttgtggga attgacacca taagatgcac attttggaca
    27601 cccactttta gagtccaaca tacttttgaa tgtagagcaa gtaaccctcc cgtgtatagg
    27661 acagacgatt accgtcgggt aacgaacgcc attgaactcc acaagattca aatgtggaaa
    27721 tttagaggtt agcttagaaa ctgcttgagt aaatgtaagt gccatagtgg ttgtgaactt
    27781 actttaccaa cctataacga tacctaatca atcggtcaaa cggagtgcgt ccgcttaaac
    27841 gtacagctaa agtgctgttg gagcaaccga taaatttact ggcagccagt gtacttgcaa
    27901 actcgtagat ttcccctgtc tctgtgtcct ccaacctaat ggcaacggcg tttggagagg
    27961 gcttttcggg atttcgagta cattcaggac atcctgttga ggatttcatg gcagcttgta
    28021 gattcccgta aacaaccgac ccatgcaaag ggcattggat tgttaccggt tcgtagtttt
    28081 tagtgaactt caaaaccgtc ttatcaggaa atttactgga aaaccttaac tgaacatctt
    28141 gtagcgagag tgattttggc attatagacc tttctaaaat gaaagcatta tacaaccaaa
    28201 atatagaaat tttcaaattt tctctgaacg cactggtatc cgatggagct aaatgtaaag
    28261 aaaaatggta tacgtcttta ctaaatcttt acacatttta actgtatagt gtttgacaaa
    28321 caacaggcat aggcgcataa tgcacccgta gttagataga tagtaaggag ataaccaatg
    28381 acttagcacc tgcccccctc tctctcgcgc accccgcccc tcgcttgcgg ctaatgggct
    28441 ttgcgcgaca tatacacttt catctcctgc atggcttaac tgcctatgca atagtacaca
    28501 catcaacaaa tcaggctggc acgtcttggc attggctgac gtaccattgt agcccgaaat
    28561 gttgataccc tactatttat gaccaacggc ggattagggc ttcagacggc aattttactg
    28621 caaaacctta tgcgaacgga tgatgcagca caatcgtttc ttcgcggtcg gggccggtgg
    28681 agacgatggc gaccggcgca ccgcagactt cttcaatccg tttcaaatat gctttggcat
    28741 tggcaggtaa tgcgtcgtag cttttcacgc cgaaagtgga ttcgctccaa ccgggcatgg
    28801 tttcgtaaat cggcttgcag gcttctaccg catcagagcc gcaaggcagg atgtcggttt
    28861 tgccgccgtc gggcaattcg tagccgacgc agatattgat ggtttcaacg ccgtccatta
    28921 catcgagttt ggtaatacac ataccggaaa tgccgttgat ttggatggag cgtttcaggg
    28981 cggcggcatc aaaccagccg cagcggcgtg cgcgtccggt taccgaaccg aattcgtgtc
    29041 cgcgttctgc caaacctgcg ccgacttcgt cgaacaattc ggtcgggaac gggcccgaac
    29101 cgacgcgcgt ggtataggct ttgacgatgc ccaaaacata atccagcatt tgagggccta
    29161 cgcccgcgcc tgccgaagcc gcgccggcga ggcagttgga cgaagtaacg aaggggtaag
    29221 tgccgtagtc gatgtccaac aacgcacctt gcgcgccttc aaacagcagt ttttcgccgt
    29281 ttttgttttt ttcgttcaac acgcgggaca cgtcggtaat catcggcgta atgcgcggcg
    29341 cgactttttc gataaccgcc atcacgtctt ccgctttcac cgactcggca ttgtgcagat
    29401 gttgcagttg gacgttgtaa taagcaagga cggcatccag tttttcacgc agtttttcag
    29461 gatgcagcaa atcggcggcg cgaatggcgc ggcgtgccac tttgtcttcg taggcagggc
    29521 cgatgccgcg gccggtcgtg ccgattttgc ctttgccgcg cgatgcttct cgggcttggt
    29581 cgagcgcgat gtgataaggc aggatcagcg ggcaggtcgg cgcgattttc agacggcctt
    29641 cgacgttttt cacgcctgcc gcgttcaact cgtcgatttc gcccaacagg gcttcggggg
    29701 agacgacaac gcccgaaccg atgaagcagt ccagactttc atgcaggatg ccgctcggaa
    29761 tcaggcgcaa aatggttttt ttgccgccga caaccaaggt atgacccgca ttgtggccgc
    29821 cttggaagcg caccacgcct ccggcttctt ccgccagcca gtcaacgatt ttacctttac
    29881 cctcgtcgcc ccactgtgcg ccgattacta caacattttt agccatagcc atataacctt
    29941 cgatattaaa aattattgcg gaaatcccca aaccggtgcg gctttgcgcc ccgccacgat
    30001 ttgcctttca tccgccatga acgggtattt agcgtttcac gacctgccaa acgccgtccg
    30061 tctttttcag acggcctgca agctcttccg aaacattgtg tccgataccg taatcgatta
    30121 cgacacactg cccttgttca cgcaaggctt cgaccgcttc gtgcgccgct tcggcatctt
    30181 ccgcatcgac caacacggcg ggctgccgtt cgatggcggg caaacgcccg ataaagctgc
    30241 gcaagtcgaa actgaatccc gttgccgggc gcgccctgcc gaaatatccg cccaatccgt
    30301 cataacgccc gccgcgcgcg accgcgtcgt ggaaatcggc ggcataggcg gcatacagca
    30361 agcccgtgtg gtaattgtcg acgcgcagct cggacaagtc gatatggatt tcacaatcgg
    30421 ggaatgcgtc gcacaccgcc tgcaattcgc ccaacgcgcc gccgaccgcc gacaaatccg
    30481 gcaaccgtcc gcgcgcgtcg gacaacactt cacgcccgcc gtacaggcgc ggcagcagcg
    30541 agaatgcttt tgcccacata ccgtccagct tccaagcctt gacctgcgct tcgaccgtct
    30601 cggtatcttt atcctgcatc aaggcaagca gcgttgcaga ctgccccgca tccaaatgcg
    30661 ccgcatcgga caaggcgcga aatatgccga tatgccccag cgaaagcagc actttgccca
    30721 tatcggcaat tttcatgctt ttcagcatca ggtctatcag ctcgatgtcg ccacggatgt
    30781 cggcaaaacc gtacatttcc gcccctgcct gcaagggttc gcgcatattc agcagaccgt
    30841 cggactgcgc gtgcaacacc ggaccggcat aacacaaccg gttaatccct tggttggcgg
    30901 ataaaagatg ggcatcgata cgcgccacct gcggcgtgat gtcggcgcgt atgcccaact
    30961 gcctgccgct gagcctgtcc gttaccaaaa tggttttcag ggaaagcccc gcatcgatat
    31021 gcgtcagcag ggaatgtgcg tactccatca gcggaggctg taccagttca taaccgtgta
    31081 cgcggaacag tgccaacaac tgctccctcg cgctttcaag ctgccgcgcg ttcgtgggca
    31141 gtacgtcggc gatatgttcc ggaagctgcc atgtctgcat gagaatctgc cttctatttt
    31201 atcctgtaaa cacaaaagac tgcccgatcc gcaatcagac ggcatctttg ccgcaacgca
    31261 tcacgccgct gtcaaaacgg cggtgcttcc ttccgattca gaatccgaac agcccctgtc
    31321 cgacggctca atgccgtcaa gtcgttaaaa ccaaacttta ccataaaata cacacaatct
    31381 gaatatactt ttataccctg tatccgccgt ctgacggcac ggcatttttt tactatataa
    31441 tgtgcgccat tatttctcat cacttcctgc tgccgttttc tgcttcagac ggcatttttg
    31501 ttgattgaac acttatgctg ctcgacctca accgcttttc cttttccgtc ttcctgaaag
    31561 aaatccgcct gctgaccgcc cttgccctgc ccatgctgtt ggcgcaagtc gcacaggtgg
    31621 gcatcggttt tgtcgatact gtgatggcgg gcggtgcggg caaggaagac ttggcggcgg
    31681 tggctttggg cagcagcgcg tttgccacgg tttatattac ctttatgggc attatggcgg
    31741 cgctgaaccc gatgattgcc cagctttacg gcgcaggtaa aaccgacgaa gtgggcgaaa
    31801 cggggcggca ggggatttgg ttcgggctgt ttttgggcgt gttcggcatg gtcttgatgt
    31861 gggcggcgat tacgccgttc cgcaactggc tgaccttgag cgattatgtg gaaggcacaa
    31921 tggcgcagta tatgctgttc accagcttgg cgatgccggc ggcaatggta caccgcgcac
    31981 tgcacgccta cgcctccagc ctgaaccgcc cgcgcctgat tatgttggtc agctttgcgg
    32041 cgtttgtgtt gaacgtgccg ctgaactata ttttcgttta cggcaaattc ggtatgcccg
    32101 ctttgggcgg cgcaggctgc ggactggcga cgatggcggt gttttggttc agcgcgctgg
    32161 cattgtggat ttatatcgcc aaggaaaatt tcttccgccc attcggactg acggcgaaat
    32221 tcggcaaacc ggattgggcg gtgttcaaac agatttggaa aatcggcgca cccatcgggc
    32281 tgtcttattt tttggaagcc agcgcgtttt cgtttatcgt gtttttgatt gcgcctttcg
    32341 gcgaggatta tgtggcggcg cagcaggtcg gcatcagttt gtcggggatt ctctatatga
    32401 ttccgcaaag cgtcggctcg gcggggacgg tgcgcatcgg cttttcgctt gggcggcgcg
    32461 aattttcgcg ggcgcgttat atttcgggcg tgtcgctggt gttaggatgg atgctcgccg
    32521 tgattaccgt gctttccttg gtattattcc gttcgccgct ggtaagtatg tacaacaatg
    32581 atccggcggt tttaagcatc gccgccaccg tcttactgtt cgccggcttg ttccaaccgg
    32641 cagacttcac ccaatgtatc gcctcctacg ccttgcgcgg ctacaaagtt acaaaggtgc
    32701 cgatgttcat ccacgccgcc gccttttggg gctgcggtct gctgccgggc tacctgctcg
    32761 cctaccgttt cgatatgggc atttacggct tctggacggc attgattgcc tcgctcacca
    32821 tcgccgccat cgccttggtg tggtgcttgg aattgtgcag tagggagatg gtcagatcgc
    32881 ataaggctgt ctgaaaactc agtacacttc aattcaaata cagttaaagt tcaaaccatg
    32941 caacccatcc gataccgaac cgacctcacc ccctacaaca ccttcggtct tcgcgcccaa
    33001 gcccgagcct ttattgcgct tgaacacgcc gacgggttgc gcgacatcgt ccggctaccc
    33061 gagttcaacc gagatactgt tttatggctg ggcggcggca gcaatatcct tttgatggag
    33121 gactacgccg gtctggtcgt acatatggaa aacaaaggca tacgcgaaat tgctcgttca
    33181 gacggcatgg ttctgattga agcgcaggcg ggcgaaatct ggcacgattt tgtcctgcac
    33241 gccgtcgcgc tgggtttgaa cggtttggaa aacctgagcc tgattccggg tacggtcggc
    33301 gcgtcgcccg tgcagaacat cggcgcatac ggcgtagagg cgaaagatgt gattcacagc
    33361 gtgcgctgct tcgatttgga tacggaaacc tttgtaaccc tttccaatgc cgactgccgc
    33421 ttcgcctacc gcgaaagcct gttcaagcag gaaggtaaag ggcgttatgt gattgtttcg
    33481 gtcgtatttg cattaaaaac gcattttgtg ccgaacttgg gttacggcga tttggcggcc
    33541 gccgttgccg aactgagcgc gggcagggtg ccgacggcga aagatgtttc cgatgcagtg
    33601 tgtgcaatcc gcaacagtaa acttcctaat cctaacgtgc ttggcaatgt cggcagtttc
    33661 tttaaaaacc ccgtcgtcag cgcagaaaaa gccgccacct tgttgcagcg gcatcctgat
    33721 atgccgcgct atccgcagcc cgacggttcg gtcaaactcg ccgccggctg gctgatcgac
    33781 caatgtcgtc tgaaaggctt tcaaatcggc ggcgcggcgg tacatgacag gcaggctttg
    33841 gtcttagtga acaaaaacaa tgcctcggca aacgatgtcc ggcagttggc gcaacacatc
    33901 aaatttacag tatttgctcg gtttcaggta gaattgcacg ccgaacccaa ttggctgcct
    33961 gcttcgttca gcctgtaaat ccaaggagta tgcccgtgac ccgccccgcc aaaatcaata
    34021 cttacacacg catcatcgac gccagccttg cgctcttcaa cgaggaaggc gagcgcaaca
    34081 tcagcaccaa ccatatcgcg gcgcatttgg gcatcagtcc gggcaatctc tattaccact
    34141 tccgcaacaa agacgaaatc atcgtccagc tcttcaaacg ttacagcgaa gccctgcttg
    34201 cctacctgaa tgaagccgtg ttgccgtctg atgtggaaga ctccatcaat tatatggccg
    34261 gtatttatga tgtgatgtgg gaataccgct tcctattcag cgacgtgaac accctgcttg
    34321 cacgcagtgc cgaactattg ggcgaacaca ataccttcac ccaagccaaa gtctccccgc
    34381 tcttggtcaa cctgctgacc cagctcaacg gtctgaacat catccaagcc gaccaaaccg
    34441 ccatgaacga tctcgccgtc aatatgtgga tggtcacgaa atactggttc gacttcgaca
    34501 gctccctgcg cggccgcacc aagctgaccg aagactccaa agcacgcggc atccgccgca
    34561 ccttaagcct ccttcgtcct tatcttttgc ccgaacaccg cgagaaatac gaccggaaaa
    34621 tcggcaacgg caacccgtaa acccacagcg tccgaatcag ccttcagacg gcacttaccc
    34681 gacggtatca gcgaatgcgc gcagtccccc cgccccatat ctccatcaca ggcctaggtt
    34741 acctcggcct gccgctggca caaaagtttt accaacacag cagccgcgtc gccgccataa
    34801 aacgcagcct gacttcggac gacatcaacc tgcccataca tctcgacacc ttcgacctcg
    34861 gcagcagcga tgcctttcag acggcattgt ggcggcacca cgccgacaaa cccgtctggt
    34921 tcttcctgct accgccctcc tcgctgacgc attacgccga caccgtcaaa caatgggcag
    34981 agcttgcccg ggcgtgcaac gtgcaacacc tgattttcac gagcagcacc agcgtttacg
    35041 gcgataaggc gcgcgaatgc gacgaaaccg ccctacccga tccgcaaacc gagtctgtcc
    35101 gccaaatcct cgccgccgaa caacacctgc tcgacagcgg catctccaac atcgacatcc
    35161 tgcgacttgg tgggctttat tgcgccgaac gccatcccgt cagccgcctt gttcaaaagc
    35221 aaaacatcct gggcggcaac cgccccatca acatcgtcca ccgtaatatc gccgtcgaaa
    35281 gcctgtttca gacggcattt aaccccggcg gcaggcggct gaaaaacatt atcgaaccgc
    35341 gccacccgac acgacgcgaa ttctatacgg aagaagccgc caaactcggc ttgccggctc
    35401 ccgatttcgc acccgacgac agcgtgggca aaatcatccg taccgtttgc gataacggct
    35461 taagcctgta aaataagcgt caacagcaac aaacaggcat tctccgcccc gatcagaaaa
    35521 tatgtccgcc ctcctcccca tcatcaaccg cctgattctg caaagcccgg acagccgctc
    35581 ggaacttgcc gcctttgcag gcaaaacact gaccctgaac attgccgggc tgaaactggc
    35641 gggacgcatc acggaagacg gtttgctctc ggcgggaaac ggctttgcag acaccgaaat
    35701 caccttccgc aacagcgcgg tacagaaaat cctccaaggc ggcgaacccg gggcgggcga
    35761 catcgggctc gaaggcgacc tcatcctcgg catcgcggta ctgtccctgc tcggcagcct
    35821 gcgttcccgc gcatcggacg aattggcacg gattttcggc acgcaggcag acatcggcag
    35881 ccgtgccgcc gacatcggac acggcatcaa acaaatcggc aggaacatcg ccgaacaaat
    35941 cggcggattt tcccgcgaat ccgagtccgc aaacatcggc aacgaagccc ttgccgactg
    36001 cctcgacgaa ataagcagac tgcgcgacgg cgtggtacgc ctcaacgaac gcctcgaccg
    36061 gctcgaacgc gatatttgga tagactaacc ttcagacggc atccgacatg aacagccctt
    36121 ttcacaacat cggcatcgta acccgcccca acacgcccga catccaagac accgcgcaca
    36181 cgctgattac ctttttgaag cagcacggct ttaccgtcta tctcgacgaa gtcggcataa
    36241 aggaaggctg catctatacc caagacaccg tcggctgcca tatcgtcaac aagaccgaac
    36301 tggggcaata ctgcgacctg gtcgccgttt taggcggaga cggcaccttt ctctccgtcg
    36361 cccgcgaaat cgccctgcgc gccgttccga ttatcggcat caaccaaggg catttgggct
    36421 tcctgaccca aattccccgc gaatatatga cggacaagct attgcccgtt ttagaaggga
    36481 aataccttgc cgaagagcgc atcctgattg aggccgcact catccgcgaa ggcaaaaccg
    36541 ccgaacgcgc catcgccctc aacgatgccg tcctctcccg tggcggcgct ggacagatga
    36601 ttgagtttga agtcttcgtc aatcgggaat tcgtctatac ccagcgttcg gacgggctga
    36661 ttgtctccac ccccaccgga tcgaccgcct attcgcttgc cgccggcggc cccatcatgc
    36721 aggcaggatt acacgccttc acactcgtac ccatctgccc gcaatccatg accaaccgcc
    36781 ccatcgccat tcccgacacg tctgaaatcg aaatcctcgt tacccaaggc ggcgacgcgc
    36841 gcgtccattt cgacggtcaa acccatatcg acgtgcaaaa cctcgaccgc atcaccatcc
    36901 gccgctaccg aaatccctta cgcattttgc acccgaccga ctaccaatat ttcaaaaccc
    36961 tgcgccaaaa actgcactgg ggcgaacaat tggtctgaac cgacccttac cggaaaacac
    37021 ctatgaatcc aaaaactccc cccgaccata cagaagatgc cgtgcgcctc tccaaacgca
    37081 tggcgcaact ggggctttgt tcgcgccgcg aagccgacgg ctacatcgaa cagggctggg
    37141 taacggtcaa cggcaaaacc gccgtactcg gtcagaaagt ttcaccggca gaccgtatcg
    37201 aactgaacaa gaaagcccac gaacagcagg cggcacgcat taccatcctg ttgaacaaac
    37261 ccgtcggcta tgtcagcgca caagcggaaa aaggctataa atccgccgcc gaactgatta
    37321 cccctgaaaa tcactgggaa ggcgataccg gccgcatccg tttcgatccg aaacacaaaa
    37381 tcggcctcgc ccccgccggc aggctggaca tcgactcggt cggattgctg gtattgactc
    37441 aggacggccg tatcgccaag cagcttatcg gcgaaaacag cggcagtgaa aaagaatatt
    37501 tggtgcgcgt gcgcggcaaa ttggacgaaa aaggacttgc cttactgaat cacggattga
    37561 gtttggacgg cgagaaactg cgtcccgccc aagtagaatg gcaaaacgaa gaccaactgc
    37621 gtttcgtatt gaaacaaggt aaaaagcggc aaatccgccg tatgtgcgaa ctggtcggac
    37681 tgcgcgtcgt cgggctgaaa cgcatccgca tgggtaaggt caagctcggc aggctgccgc
    37741 ccggcaaatg gcgttatctt gctcccggcg aatcgtttta aataagcatt aaaaaatgcc
    37801 gtctgaacgt ccttacgttt tcagacggca ttgccttatg cccaaataac ggtttcttcc
    37861 aagggcctgc gcgctttcgt ggcgatttcc tgaacgcggt agccgaatgt cgcggcaacg
    37921 gacacgcccc attctgccgc atcgaacaaa ccgaactgcc cggacaatac gcgttccata
    37981 tcggcatagt tgaaaccttc caccgggcag gaatcgatac ctgccatcgc cgcacccgtc
    38041 atcatgttgc ccaacgcgat ataggtctgg cggcaacacc agtcaaacaa ggcgcgcgaa
    38101 tcgtccaaaa tcttgatgtc gtcggcttga aacgcctgat acctcgccaa agattttgcc
    38161 atggcatccg gttcggtaac gccgcgccgt ttgaggcttt ccaacataaa cgggctgtcg
    38221 aagcgggcat ttttctttgc caaaaacacc accaaatgac tggcggtatc caaagcatcc
    38281 gccatacccc aagaaaacgg cttgattgcc tgtcggattt cggggttttg aaccacaaca
    38341 aactgccaag gctccgaacc gaccgaactg ggcgacaaac gcccgagttc taaaataaac
    38401 tgaaaatcct cggcactgat tttacgtgcc gcatcgtaat gccggcatga tttgcggttt
    38461 ttaaatgcgg atagaacctg ctctttgctt aatactgcca tcgcacatcc tttcattact
    38521 tgtattgcaa attgatttca aaccgatacg gcggcagatt cgccaataac gcccaaacgc
    38581 ctttcagacg gcatttggtt catcgcaaac acgcctacgg gtaggcgata taggcataag
    38641 ccgaatggtt gtggatagac tcgaaattct cgctctcgac gacgaaactc ttgatgcgtt
    38701 tgtcggcgat cagcgaagta gcgacatcgc gcaccatatc ttccacgaat ttcgggtttt
    38761 cgtaggcctt ttcggtaacg tatttttcat cggggcgttt gagcaggccg tagagttggc
    38821 agctcgcctg cgcctccaca taatcgatga cttcctcgat accgacttcg gcatcggcag
    38881 tcaggctgac ggtaacgtgc gaacgctggt tgtgcgcgcc gtattgggaa atttctttgg
    38941 aacacgggca aagcgaggtt acgggaatca tgaccttcat actgtggccg tatgcaccgt
    39001 ctttcatttc gcccgtgagg cagacatcat aatccagtaa agactgaata ccggaaaccg
    39061 gggcggtttt cttgcggaaa aatgggaaag aaacgctgat tttaccggcg cgggaatcca
    39121 aaagcgcgac catttcggca gtcagcctat gcaattgtgc gaaatccaag gcttcagaat
    39181 attgctccat caatgcgaca aagcgcgaca tatgcgtacc cttctgctcg gcgggcaggt
    39241 aaaccgtcat ggtcagacgg gcaatggtgg actggatgcc ttctgcagtt tgcagggtaa
    39301 tcggaaagcg caggtctttg ataccgacct gattgatcgg cagattgcgc aaatctcggc
    39361 tggattgcac gtctgcaata gtgttcatga gttgtttttc cttaatattc atgtaattgt
    39421 tgagatgtgt gcatggtatg gcgtttccgc cggggaatat accgtcaatc tgcaaatgcg
    39481 agattatatc actcgctttt agcagctgca ttgattgttt ctatttttca gatagtgata
    39541 aaatcttcga ttattcacat ctacataggc agagaccaaa tgaaattcgc cacgaaagcc
    39601 attcattcca gctacgattg cgacgaacac aaccgcgcgc tgatgccgcc gatttatcaa
    39661 aacagtatgt ttgcgttgca cgagattggc gaaaatgtgc cttaccgtta ttcgcgcctg
    39721 agcaacccga cccgtcagat tttagaagac accgttgccg atttggaaca cggtgcggca
    39781 ggttttgcgt tttccagcgg tatggcggga attgatgccg tatggcgcac tttcctgcgc
    39841 ccgggcgata ccattgtcgc cgtcgccgat atttacggcg gcgcttatga tttattggtc
    39901 gatgtttatc aaaaatgggg ggtgaacgtt gtttttgccg atttaggcaa tccggataat
    39961 ttggacgaac tgcttaaagc gcacaaggtc aaactggttt ggctggaaac gccgtccaat
    40021 ccacttttac gcttggtaga catcaaagtc cttgccgcaa aagccaaagc agccggtgcg
    40081 ctggtcggta tcgacaacac ttttgccacg ccgtatctgc aacagccgtt ggatatgggt
    40141 tgcgattttg tattccattc cgctaccaaa tatttgtgcg gccattccga cgtgttgatg
    40201 ggcatcgtcg ttgccaaaac caaagaactg gcgcagcctt tgcacgatat gatggtgcat
    40261 accggcgcgg ttgccggccc gctggactgc tggctggtgt tgcgcggcat caaaacactg
    40321 gctctgcgca tgaacgccca ttgccaaaac gcactcgaaa tcgcgcgccg tttggaagcc
    40381 catcctgcca ttgaaaaagt gttccatccc ggcctgccgt ctcacgaaca ttacgaactg
    40441 gcgaaaacac aaatgcccaa aggcatcggc ggcgtggtta cggtttatct caaaaacgac
    40501 acgcgtgaag cggcaaacag cgtgattaaa aacatgaaac tggtcaaaat ggcttccagc
    40561 ctcggcggtg tggaaagttt ggtcaaccat tgctattccc agtcccacag cggcgtaccg
    40621 catgatgtga aaatggaaat gggcatcaaa gtcggtctgc tgcgtttctc catcggcatc
    40681 gaagacgcgg acgatatttg gaacgatatt tccgccgcac tcgatacgac tttgtaaact
    40741 gtaaaaatgc cgtctgaaac catggtttca gacggcattt caattaaccc ggccgaaaat
    40801 caacgcttca acatctttgc cgcctcaata gcgtaatagg tcaaaatccc gtccgcaccc
    40861 gcacgtttga atgccagcag gctttccaaa accactttgc cgccgtccag ccagccgttg
    40921 gcaatcgctg cctgcaacat cgcgtattct cccgaaacct gataggcata agtcggtacg
    40981 ccgaactcgt ccttcacgcg gcggacaacg tccaaatacg gtaaaccggg cttcaccatc
    41041 accatatccg caccttcccg aatatcgagc gccacttcat tcagcgcctc atcggtattt
    41101 gccggatcca tctggtaggt tttcttgtcc gccttgccca aattgcccga actgcccacc
    41161 gcatcgcgga acggcccgta aaacgccgaa gcatatttgg cagaatacgc cataatccgc
    41221 gtatggatat gcccggcatc ctccaacgcc tcgcgaatcg caccgatacg cccgtccatc
    41281 atatcggaag gggcaaccac ctgcgcgccc gcttcagcgt ggcacaaagc ctgcttgacc
    41341 aaaacctcta cggtttcatc gttcatcaca taaccgtttt cgtccgtcag cccgtcctga
    41401 ccgtgaaccg tataaggatc gagcgcgaca tccgtcataa tgcccagttc gggaaacctc
    41461 tcgcgcaagg cgcggacagt tgacggcacg agtccttcgg gattgtacgc ctcctgcgca
    41521 cgctcggttt tgtttgccgt aaccacgggg aacagtgcca acatcggaat accgagcttt
    41581 accgcctctt ccgctgtaaa cagcagcctg tccaaacttt gacgcttcac acccggcata
    41641 gaaggcacat cctcctcgcg cgccgacccc tccaatacga acaccggata aatcaaatca
    41701 tcggcggtca gcgtgtgttc gcgcatcagg cggcgtgaaa aatcgtccct gcgcatacgg
    41761 cgcatacgcg aagccggaac attgcggtaa ggaaactgca taagccctcc aatcatcctg
    41821 tcaacaattc aaaccatacg gaagccgccg caacttgcag acagcatcgg gcggcaatca
    41881 aaatccgttt tcaccggaaa gggtttcggc ccgccgaaat gccgcgccga aggacggcgt
    41941 cccgcccatt aaagaacaat agcaaaacaa cagcttcagg ctgtcagcct tcgatattct
    42001 tacgccacaa taccaaaagt ttcccgatat gctgaacagg ttgcgcgtca accgcctcac
    42061 ataaggcagt gcagatttcg atacgttcgg cacggtcgtc gccgaatacg cgcactttaa
    42121 tcagctcatg cgccgtcaat gccgcatcgg tttccttgat gaccgcgtcc gtcagaccct
    42181 gctgaccgac catcacaaca ggatggagat ggtgcgcgcg cgctttcagt tccaaaattt
    42241 ctttggtgtt caatttggta tccgtcattt tcctactgct tgaataaaaa gaataatgcg
    42301 gcattgtacg cgatttggca cgacatcggc aaaattaacg tacaatacgc ggtttccatt
    42361 ccgacccgcg aaccactatg gctgtacgtt ccaaatcctc aaaagcgtgg ctgcacgaac
    42421 acgtcaacga ccactacgtc catatggcgc aaaaagacgg ctaccgcgcc cgtgccgcat
    42481 acaaactttt ggaaatcaac gaaaaagaca aattaatcaa acccggcacg gtacttgccg
    42541 acttgggcag cgcaccggga agctggtcgc aggttgccgc caagctgacg ggtacttccg
    42601 gagcagtttt cgccttggac atcctgccta tggaagccat agggggcgtc tccttcattc
    42661 agggcgactt ccgcgagaac gacgtactgg cacaattcga aaccttgttg gacaaccgcc
    42721 cgctcgacct tgtaatttgc gatatggcgc ccaatatgtc gggaaacgcc gtaagcgatc
    42781 aggcacgcag cttttatctg tgcgaactgg ctttagactt cgcctcgcaa cacctgaaaa
    42841 ccggcggcag ctttttggtc aaagtctttc agggtgcagg ctatcaggaa tacatggcag
    42901 ccatgcgcga aattttcggc acggtgcaga cgcgcaaacc cgaagcctcg cgcaatcgct
    42961 ccagtgagat ttatttattg ggcaaaaata aacgctgaca atacagacgg cgtgctttac
    43021 aatcatttcc gttttacacc tcaattatgg agccttgcta agtggggaac acctttaaat
    43081 caatccttgt ctgggtcgcc ttgggtatcg gcctgatggc tgcgttcaac gctttagacg
    43141 gtaaaaaaga agacaacggg caaatcgaat attctcagtt catccaacag gtcaacaacg
    43201 gcgaagtatc cggcgtcaac atcgaaggat ccgtcgtcag cggctacctg attaagggcg
    43261 agcgcaccga caaaagcacc ttcttcacca acgcgccttt ggacgacaac ctgattaaaa
    43321 cactgctcga caaaaacgtc cgtgtaaaag taacgccgga agaaaaaccg agcgcgctgg
    43381 ctgccctgtt ttacagcctg ctgcccgtcc tgctgctgat tggcgcgtgg ttctacttta
    43441 tgcgtatgca gacgggcggc ggcggaaaag gcggcgcatt ctcattcggc aaaagccgcg
    43501 cccgcctact ggacaaagat gccaacaaag ttacctttgc cgatgtcgcc ggctgcgacg
    43561 aagccaaaga agaagtgcag gaaatcgtcg attacctcaa agcgccgaac cgctatcaaa
    43621 gcctgggcgg gcgcgtgccg cgcggcatcc tgctggcggg cagcccgggt acgggtaaga
    43681 cgcttttggc gaaagcgatt gcaggcgaag ccggcgtgcc gttcttcagc atttcaggtt
    43741 ccgactttgt cgaaatgttc gtcggtgtcg gtgcaagccg cgtccgcgat atgttcgagc
    43801 aggcgaagaa aaacgccccc tgcatcatct ttatcgacga gattgacgca gtcggccgcc
    43861 aacgcggcgc aggtttgggc ggcggtaatg atgagcgcga gcaaacatta aaccaattgt
    43921 tggttgaaat ggacggtttt gagagcaatc agactgtaat tgtgattgcg gcaaccaacc
    43981 gccccgacgt actcgatcct gcgctgcaac gccccggccg tttcgaccgc caagtggttg
    44041 tccccctgcc ggacatccgg gggcgcgaac agattttgaa cgtccactct aaaaaagtgc
    44101 ctttggacaa atctgtggat ttattgtccc tcgcgcgcgg cacgccgggt ttttccggcg
    44161 cggatttggc gaacttggtc aacgaagccg ccctgtttgc cggccgccgc aataaagtca
    44221 aagtcgatca gagcgatttt gaagacgcca aagacaaaat ctatatgggt ccggaacgcc
    44281 gcagtatggt gatgcacgaa gacgaaaaac gtgcgacggc gtatcacgaa tccggacacg
    44341 cgattgttgc cgaaagcctg ccctttaccg accccgtcca caaagtaacc attatgccgc
    44401 gcggacgtgc gctgggtctg acttggcagc ttccggagcg cgaccgcatc agtatgtata
    44461 aagatcagat gttgagccag ctctccatcc tgttcggcgg acggattgcc gaagacatct
    44521 tcgtcggacg catctccacc ggcgcatcaa acgactttga acgcgcaacc caaatggcgc
    44581 gcgaaatggt aacgcgctac ggcatgagcg acaaaatggg cgtgatggtt tatgcggaaa
    44641 acgaaggcga agtcttcttg ggacgcagcg taacccgttc gcaaaacatt tccgagaaaa
    44701 cccagcagga catcgatgcg gaaatccgcc ggattttgga cgagcagtat caggttgcct
    44761 acaaaatcct cgatgaaaac cgcgacaaga tggaaacgat gtgcaaagcc ctgatggaat
    44821 gggaaaccat cgaccgcgat caggtactgg aaatcatggc gggcaaacaa cccagcccgc
    44881 ccaaagatta cagccacaac ctgcgcgaga atgcggacgc ggcggaagat aacgcgccgc
    44941 acgctccgac tcgggaagaa accgaagcac ctgccccggc agacaccgct tcgacagagt
    45001 ccgagcagca gcctgaaaac aaggcttaac ttcccgaaca aacggcagcc cgcaagctgc
    45061 cgttttccca tcctgttttc agacggcatt ttccgctcaa tgccgtctga aacgccaaag
    45121 ccgtatcggt ttttgcgaat ctgcgatatg gcggtttaaa ttgaaatcag aacggggcaa
    45181 ggctgtaccg gtttaaaatt aaaccgctat aataccgccc ttcgaataca cgcccgcatt
    45241 ggtgatgccc gtgaaaaaag ttgaaaaaaa catcttggtg ctgcacggcg cggacaaaat
    45301 gttcgagctg gtcgataagg ttgaggatta cccgaacttt ctgccgtggt acagcaaaac
    45361 cgaggtcatc ggacgcaacg gcaacgaact gaaggcgcgg ctgtttatgg actatatgca
    45421 cgttcgccaa tcattcgcca cgcacaaccg caacattccg ggcagggaaa tccgtatgga
    45481 actgctcgaa ggtccgttca aaaccttacg tggaacgtgg aaatttatcg atttgggcga
    45541 cgatatgtgc aaaatcgaat tcaatttgga atacgatttt tccaatgccg ttttgtccgc
    45601 cttaatttcc cccgtcttca accacctttc cgccacgctg gtcgaagcgt tcgtcaaaga
    45661 ggcagaccgc cgttatgctt gaaattgaga ttgtgtacgg gctgcccgac cgacaggttt
    45721 tgaaaaccat gcagcttgcc gagggaacaa ccgtccgcgc cgccgcactg caaagcggtt
    45781 tggacggcat atttgaagat ttaaacctgc attccgcgcc tttgggcatt ttcggcaaag
    45841 ccgtcaaaga cgacacgccg ctgcgcgacg gcgaccgcat cgaagtgtac cgcccgctgt
    45901 tgatcgaccc caaagaagcg cgccgcaaac gcgttcaaaa tcaagaagaa taaccatgcc
    45961 gtctgaagcc ttcagacggc attcggcaga aacacggaaa atatcatgtc aaacacaatc
    46021 aaaatggttg tcggcttggg caacccgggc aaagaatacg aacagacccg ccataatgca
    46081 ggcttttggt tcctcgacga actggcttgg aagtggaagg cttcgtttaa agaagaaaaa
    46141 aaattcttcg gcgaagtcgc ccgtgctacc ctgcccgacg gcgatgtctg gctgctcaag
    46201 ccgaccacgt tcatgaaccg ttccggacag gcagttgccg cccttgcgca gttttataaa
    46261 atcaaacccg aagaaatcct cgtcgtccac gacgaactcg acattccctg cggacggatc
    46321 aaattcaaac tcggcggcgg caacggtgga cacaacggct tgaaagacat tcaggcacgg
    46381 ctcggcacgg ccgactatta ccgcctgcgc ctcggtatcg accacccggg cgaccgcaac
    46441 ctcgtcgtcg gctatgtcct gaacaaaccc agtacggaac accgccgaca gattgacgat
    46501 gccgtcgcca aatccctgca ggccataccc gacatccttg ccggcaaatg ggaagaggca
    46561 acccgcttcc tgcacagcaa atgacccgat gccgtctgaa gccctttcag acggcatgtt
    46621 cccgatttcc atatccgaac agtcatgacc gaactcaagc agcttatcca aaccgaatcc
    46681 atcctcgtca tcgaagaaac cctcgatttc ctgctgtacg aatgcagcat cgacgacgca
    46741 ccatccgccg aagaagtggc acaatggcgc gacatacttg ccgcacgcgg cggcaaattc
    46801 ctgcgcctgt ccaaaatctg ccaaacgtgg ctggacgagg aggcggcatg aagctgccgc
    46861 gcaaccgctt cagcctgctt tccgcattgt ggtttgccgg cggcatctat tcgctgctct
    46921 tcaaagctgc cgacaccgcg ccgccgccgt ttccgcattt cgacaaagca gcacaccttg
    46981 ccctgttttt cgcacaaatc tggcttttga ccaaagcatt caaaaccgga aaacttccca
    47041 tcccctaccg cagcctgatg gtctttgccc tctgtttcgc cctcttcagc gaatgcgcgc
    47101 aggcatggtt taccgcaacg agaaccggca gtttgggcga tgttcttgcc gatatggcag
    47161 gtacggttct cgcactcttt gccgcccgcg ccgccgaccg cccggactga atcggtttat
    47221 ttcccccaaa caggatgcac ctcttcccga tacatcctat ttttcccctt acatatcaat
    47281 cccatctcat aaacaaaacc gacaaatcgt ttacaatata tttacactac atcagattac
    47341 aaatatactc gaaccagttg cagaatcggc ggttccacaa accgtttcgg attgcacgac
    47401 acaaaagaaa aaccgatttt attgccttaa aggagtattc atgaacctgc atgcaaagga
    47461 caaaacccag catcccgaaa acgtcgagct gctcagtgcg cagaagccga ttaccgactt
    47521 taagggcctg ctgaccacca ttatttccgc cgtcgtctgt ttcggcattt accacatcct
    47581 gccttacagc cccgatgcca ataaaggtat cgcgttgctg attttcgttg ccgcactttg
    47641 gtttaccgaa gccgtccaca ttaccgttac cgcgctgatg atccctatcc tcgccgtcgt
    47701 actcggtttc cccgacatgg acatcaaaaa ggcgatggct gatttttcca acccgattat
    47761 ctacattttt ttcggcggct tcgcgcttgc caccgccctg catatgcagc ggctggaccg
    47821 taaaatcgcc gtcgccctgc tgcggctgtc gcgcggcagt atgaaggccg ccgtgctgat
    47881 gttgttcctc gttaccgcct ttctgtccat gtggatcagc aacaccgcca ccgccgcgat
    47941 gatgctgcct ctggcaatgg gtatgctgag ccacctcgac cgggaaaaag aacgcaaaac
    48001 ctacgtcttc gtcctgctcg gcatcgccta ttgcgccagc atcggcggct tgggcacgct
    48061 cgtcggctcg ccgcccaacc tgattgccgc caaagcccta aatctggact tcgtcggctg
    48121 gatgaagctc ggcctgccga tgatgctgtt gattctgccc ttgatgctgc tctccctgta
    48181 cgtcatcctc aaacctaatt tgaacgaacg cgtggaaatc aaagccgaat ccatcccttg
    48241 gacgctgcac cgcgtgatcg cgctgttgat tttccttgcc acagccgccg cgtggatatt
    48301 cagctccaaa atcaaaaccg ccttcggcat ttccaatccc gacaccgtta tcgccctgag
    48361 tgccgccgtc gccgtcgtcg tcttcggcgt ggcgcaatgg aaggaagtcg cccgcaatac
    48421 cgactggggc gtgttgatgc tcttcggcgg cggcatcagc ctgagcacgc tgttgaaaac
    48481 atccggcgcg tccgaagcct tgggacagca ggttgccgcc accttttccg gcgcgcccgc
    48541 atttttggtg atactcatcg tcgccgcctt cattattttt ctgaccgagt tcaccagcaa
    48601 caccgcctcc gccgcattgc ttgtaccgat tttctccggc atcgctatgc agatggggct
    48661 gcccgaacaa gtcttggtat tcgtcatcgg catcggcgca tcttgtgcct tcatgctgcc
    48721 ggttgccaca ccgcctaacg cgattgtgtt cggcacgggc ttaatcaagc aacgcgaaat
    48781 gatgaatgtc ggcatactgc tgaacatcct ctgcgtagta ttggttgctc tgtgggctta
    48841 tgctgtactg atgtaaacca tcgacctaaa caacaagacc gtctgaaaga atatttttca
    48901 gacgaccttg aagtttgtcg ttcagacaca atttgtcgaa tcattcaaaa ccggattcta
    48961 acgaaaggaa acccatgatt atcctgcaca ccaacaaagg cgacatcaaa atcgaactcg
    49021 atttcgacaa agcccccgtt accgccaaaa acttcgagca atacgtcaaa gacggcttct
    49081 acgacggcgt aatcttccac cgcgtcatca aaggcttcat gattcaaggc ggcggcatgg
    49141 atgaaaacat gaacgaaaaa gaaacccgcg atccgattca aaacgaagcg tccaacggcc
    49201 tgcccaacga taaatacacc atcgccatgg cacgtacttc cgacccccat tccgctggcg
    49261 cgcaattctt catcaacacc gccgacaatg ccttcctcaa ccaccgctcc aaagaaatgt
    49321 acggcagaac cgtcgtccaa gactggggtt atgccgtatt cggcaaggta gtcggcggtt
    49381 ttgacgtggt cgatgccatc gaaggcgtat ccaccaaacg ccacggctac catgatgatg
    49441 tgccgacaga acctgtcatc atcactaaag ccgaagcggt ataaaccggc aatccgaaag
    49501 cagcctgcac aaaggctgct ttttttgttt caggcggcat aaaatccaag cacatatgcc
    49561 gtctgaacac ctcccgcaag ccttcggaca atgttgcaac gtgttaaaat agcctccttt
    49621 cctgaaacca caacaaggaa accatcatgg caaatattgc ccgcgacatc ttcaaagcct
    49681 acgacatccg aggcattgtc ggcaaaaccc tgactgacga tgccgcctat ctcatcggca
    49741 gggccatcgc cgccaaagcc gccgaaaaag gcatgacccg catcgcgctc ggacgcgacg
    49801 gacgcttgag cggtcccgaa ctgatggagc acatccaacg cggcctgacc gacagcggta
    49861 tcggcgtcct caatgtcggc atggttgcca cgcccatgct ctattttgcc gccatcaacg
    49921 aatgcggcgg cagcggcgtg atgattaccg gcagccacaa tccgcctgat tacaacggtt
    49981 tcaaaatgat gctcggcggc gacacgcttg ccggcgaagc catccaagag cttttgtcca
    50041 tcattgaaaa agacggtttt gctgccgccg gcaaacaagg cagcgtaacc gaaaaagaca
    50101 tctccggcga ataccacaac cacatcgtcg gacacgtcaa actcaaacgc ccgatgaaca
    50161 tcgctatcga cgcgggcaac ggcgtgggcg gcgcatttgc aggcaaactc taccgcgctt
    50221 tgaggtgcgg cgttaccgaa cttttctgcg acgtggacgg caatttcccc aaccaccatc
    50281 ccgacccatc caaacccaaa aacctgcaag atttgattgc cgcgctgaaa aacggcgatg
    50341 ccgaaatcgg cttggcgttt gacggcgatg ccgaccgctt gggcgtggtt accaaagacg
    50401 gcaacattat ttatcccgac cgccaactga tgctgttcgc gcaagacgtg ttgagccgca
    50461 accccggcgc gaaagtcatc ttcgacgtga agtccacccg cctgcttgcc ccgtggatta
    50521 aagaacacgg cggcgaagcc ataatggaaa aaaccggcca cagcttcatc aaatcgacca
    50581 tgaaaaaaac cggcgcactc gtcgccggcg aaatgagcgg acacatcttc ttcaaagaac
    50641 gctggttcgg attcgacgac ggtctgtacg ccggcgcacg cctcttggaa atcctgtctg
    50701 cctccgataa tccgtccgaa gtgttaaaca acctgccgca aagcatttcc acgcccgaac
    50761 tcaacatcgc cctgcccgaa ggcagcaacg gccatcaggt tatcgacgaa ctcgccgcca
    50821 aagccgaatt tgaaggcgca accgaaatca tcaccatcga cggtcggcgc gttgaatttc
    50881 ccgacggctt cggtctgatg cgtacttcca ataccacgcc gattctggtg ttgcgttttg
    50941 aggcggatac gcaagaagcc atcgagcgga ttcaaaacca attcaaagcc gtcatcgaaa
    51001 gcaatccgaa tctaatctgg cctctgtaaa cacaggaaaa atgcttaatc aaatcttggt
    51061 agattggatt tggcttaaaa atctccgcgt ccgttcgtgt ttggggtggt cgaacaactc
    51121 tttcgggctg ccctgctcta cgataacgcc gccgtccatc acgacaacgg tcgtggcaac
    51181 ttccagcgcg aacttgattt cgtgggtaac gacgaccatc gtccaacctt cccgcgccaa
    51241 ttccttcatg gcgttcaaca cgtcttgcac caactcgggg tcaagcgcgg aagtgggttc
    51301 gtcaaacaac atcagctcgg gctgaatcgc caatgctcgg gcaatgccga cgcgctgctg
    51361 ctgaccgccg gaaagctggt agggatacaa atccactttg tcgcccaagc cgactttttc
    51421 cagcagtttc agagcctctt cgcgcgcttg ggcggcaggc ttgccctgta cggcaaccgg
    51481 tccttccatc acgttttcca aggcggtttt gtgcggaaag aggttgtatt gttgaaacac
    51541 catgcctgat ttgcggcgca gtgccaaaat atcgtgtttg cttggttttt tagaaaaatc
    51601 gattttcagc ggtcgctcgt tgtcgaactc gatttgtccg tcttcgggca tttccaacgc
    51661 gtttaggcat cgcagaaacg tcgttttgcc tgagccggaa ggcccgagga tgacgaccac
    51721 ctgccctttg cacacatcca aattgatgcc gcgcaaaatg gtatttttgc cgaaggtctt
    51781 atggatattg cggattttaa tcatgacaac tccttatttg gcgacgtagc ggtcgaaacg
    51841 tttttccaaa cgcgcctgaa tcaggaacag cactttacaa aaacaccagt aaaccaaagc
    51901 ggcttcgata tagacgggca aaaagtcata agtgcggttt gccgtttcct gcgcgacgcg
    51961 gaataattcc gttaccgtca cgactgccgc gagcgaggtg tttttaaaca aaccgataaa
    52021 ctcgttgctc aaaggcggca cggcaacgcg gaatgcctgc ggcgcaacga tgcggcggaa
    52081 cgtctgcata taggtcatgc cgatggagaa acctgcttcc cattggcctt tcggtacgga
    52141 caaaattgcc gcgcgtatgg tttcggaagc atatgcgccg acattgagcg aaaagccgat
    52201 gatggcggca ggaatcgggt cgatatagat gccgacggaa ggcagcccgt aaaacacaat
    52261 cacaagctga accaacagcg gcgtaccgcg aatgacggaa atataaaatt ccaccaattt
    52321 cagcaggatt ttccgcacga tgccgccggc gggcataatc cgcaccaaag ccacggctac
    52381 cgcaatcatc ataccgataa cgaaagaagc tgccgccaaa ggcagagaga ccgcgaagcc
    52441 ggctttgacc ataggcaaaa acgcgctgac aatcatatcg gcgcgtgttt ccgtcataaa
    52501 cggcagcgaa gcaaggaaat tattgaacac tgatgtcttt tccgaagaat tgttcgccca
    52561 gttttttcag cgtaccgtcg gctttcagct cgttgattgc cgtactgaat ttcgccacgg
    52621 cttcgtcatt gcccttgttg acaatcaggc cggaaccgac tttttcatcg gcaggtgcgg
    52681 accaaacgat tttcacgccc gcattcgggt ttttcttcag atagtccaaa accgccaatt
    52741 cgtcgttcag ggttgcatcg gcacgttttt gttcaatcag ggtcagcgat tgcgccaaac
    52801 cgtccactgc caccaaatct gcgcctgcag ctttggcttt ttcgccgtag ttgctggtca
    52861 gggattgtgc ggttttcacg cctttgatgt cgtcgataga tttgatgttg ctgtcgttac
    52921 gggcaaccaa tacggcaccg ctccagctgt aaggatcgga tttgtcgaat gtcgcttggc
    52981 gttcggggct ggtcagaccg acttggtttg ccaccacgtc gaaacgcccc gccttcaaac
    53041 ccgccatcat cgaatcccat tgcgtttctt taaactcgac tttcacgccc agtttttccg
    53101 ccacggcgcg ggttacttcc acatcgtaac cggtcagttt gccgtccttg tcgtggtagg
    53161 taaacggtgc gtaagtgcct tccgtgccga cggtaaccgt gcctttattg ttgatgcgct
    53221 cgattaaaga accggaaact gccgattgtg caggcgcgga agatgctccg ctgccgcttt
    53281 ccgaaccgcc gcaggccgcc aaaaccaatg cggcaatgcc gccgagtacg aattttttca
    53341 acatcattct ctcctgtaaa aagtgtgcaa gtgcggcatt ctaagtgaaa ttgcggcaaa
    53401 cacaaagaat acttttttta tatggttatt ccaaatcgga atggaaaaga tatgccgaag
    53461 ccgccggaag cggaaataaa gggggcggga aaggcggcgg gcggcacgaa accgaaaagc
    53521 ggcatcaggt atgcaaacgg cggattcacg cacgccgttc cggcaaaaat ccgcctgccg
    53581 attcggtgct ataatccatc cgaaacagat gacaccaaaa aggaacatct catgaacacg
    53641 ccccaatccg ccatcattcc cgaccacgcc caagccggca tctttatcga agccgacttc
    53701 gccgccaacc gcctcaacga tattaaagcc gcctgccgcg cttcgctcga cgcgttatcc
    53761 gccttgaaaa cccgctttcc ggacgatatt ttgggtctga ccatcgcctt cggcagcaaa
    53821 gcctgggcaa cattcggaca tacggacgaa ggcagtgaaa tcaaaccctt ctccgaaatg
    53881 ggcaacgggc ttgcgccgtc cacgcagcac gatatgtcca tccacatcca atctttccgc
    53941 caaaacgccg cctacgcgct tgcccaatcc gttttgggcg cgttcggcga cagcatatgc
    54001 gtcgcgtccg aagaacacgg tttgcgcctg tatcaggatc gcgggctgga cggtttcgtc
    54061 gacggcacgg aaaacccgca gggcgatgaa aatgtccgcg aagtcgccat catccccgaa
    54121 ggaaaacccg atgcgggcgg cagctatgtc ctgctgcaaa aatacctgca cgatttgaaa
    54181 aaatgggatg ccgtccccgt cgccgaacag gaggcctcgg tcggacgcgg caaggaaacc
    54241 gacgacgaat tcggccacga tgtccgcctg cccgattcgc acctcggccg cgtcaacctg
    54301 aaagaaaacg gagtcggcct gaaaatcgtc cgccgcagcc tgcccttcgg caaaatcagc
    54361 ggcgaacacg gtttgatgtt taccgcatac tgccgcacgc tgcacaatat cgaagcgcaa
    54421 ttattgagta tgttcggcga cacggatggc aaaaccgacc tgctgctccg gcacctctcc
    54481 gccgccgttt cgggcggata ctactacgcg ccgtctgtcg aacgcctgca aaacctctaa
    54541 gccagacgat gccgtctgaa accatttttc agacggcatt ttcccgtttt atagtggatt
    54601 aacaaaaacc agtacggcgt tgcctcgcct tagctcaaag agaacgattc tctaaggtgc
    54661 tgaagcacca agtgaatcgg ttccgtacta tttgcactgt ctgcggcttc gtcgccttgt
    54721 cctgattttt gttaatccac tataccgtcc aaccgttttc cgagcgcgca ctatgtctgc
    54781 aagcatccaa gcattcgacc cgctaaccgt ccccatttcc ggcaccaacc tgattgaggc
    54841 ctctgccggc accggcaaaa cctacggcat tgccgccctg tttacacgcc tgatcgtatt
    54901 agaacaaaaa aacgtcgaac gcgtattggt cgtaaccttc accaaagccg ccaccgccga
    54961 gctgaaaaca cgcctgcgcg ggcgtttgga cgatgtgtta caagttttag aaagcaaagg
    55021 aattgccaaa cttggagacg acacgctttc agacggcatt gccgcctact gcgccgaaca
    55081 ccacgaaggc gacaccttcc tgcccgaact cttaaaacag gctttgcaaa aagagggccg
    55141 gacgcgtctg attgtccgcc tcaaggccgc catcgggcaa ttcgacaacg ccgccatcta
    55201 taccatccac ggcttctgcc agcgcatcct gcgcgactac gccttcctgt gccaagcacc
    55261 gttcgatgtc gaaatgaccg aagaagacgg cgaccgcctg cttatcccgg cgcaagattt
    55321 ttggcgggaa cgcgtcagca atgatccggt gcttgccgca ttggcgctta aacgcaaagc
    55381 tgtgccgcaa accgtccttg cccaaatttc gcgctatctt tcccgcccgt acctgaattt
    55441 ccgccgtccg caggcggatt tgaaacaggc gcagcgcaac gccgaaacct catggcaaac
    55501 catctgccgc ctgctgccag aactggaagc cggcttttgg cgcattcatc ccgacctcaa
    55561 cggcaacagt taccgcaaaa acagcttcgg caaccttttt aaagaactgg cacaaaaatc
    55621 cgccgccgga caactgccct ttctggacaa agacacacac gacagactct tgaaacttgc
    55681 atccgacaaa ctcgaagccg gactgaaaaa aggcaaaacg cccgatgcgg cagtatttgc
    55741 cgaattgcag aaactggcag acttcgggcg cgatttgaac gcactcgaag aagcggaaga
    55801 agcaacaatg atccggctgc aactggattt aatcgaatac ctcaaccgca gccttgccga
    55861 gatgaaaaaa tcgcgccgcg aacgcggttt cgacgacctg ctgctcgatg tccacaccgc
    55921 gctgaccgac aatccgcacg ccgacactct cgcccgcgcc gttgccgaaa actgggaaac
    55981 cgcgctgatc gacgagttcc aagacaccga cccgctgcaa tacgaaatct tccaaaagat
    56041 tttcatcgcc caaaaccgac cgctgttcct cgtcggcgat cccaaacagg cgatttacag
    56101 ctttcgcgga gcggacattt acgcctacct tcaggcggcg gaagacgcgc ggcaccgcta
    56161 cacgctcgcc accaactacc gcagccacgc cgcgcttatc ggcagcatag gcgcgctgtt
    56221 ccgcctcaaa gaacgcccgt tcgttttgga aaacatcggc tattcggaag tcggtgcggc
    56281 gcgtgccgaa agcaggctgt cccccgaacg tcctgccgta caggttcgtt ggctgcacga
    56341 aaacgacaat gaaaaagcca acaaagacgt tttgcgccgc cgtgccgccg actattgcgc
    56401 cgacgaaatc gcccacgcgc tcaacgaagc cgccagaggc cgtctgaatt tcaaaggctg
    56461 cccgttgcag tcgggcgata ttgccgtgct ggtacgcacg cacaacgagg cggtgatggt
    56521 ttccgccgcc ctgaaaaaac ggcaggtgca aagcgtcctg ctttcgcgcg aatccgtgtt
    56581 cgcctcgccc gaagccgccg ccctgtccgc actcatcggc ttctggctcg aaccgcgccg
    56641 cgccggaacg ctgcgttttg tcctgacaag cagcatattc ggttatgacg cgcagcaatt
    56701 gcacgacttc aaccaaaacg aaagcgagat tttgcattgg gcggaatctg cccgaaccgc
    56761 actcgacaac tggaataaat acggcatttt cgccgctatg cagcaatttt cccaaacaca
    56821 cggcatcgaa acgcgcctct taagccggaa caacgggcgc agtctgacca attatttcca
    56881 actgctcgaa ctgcttgccg ccgaagacgc gcaaaaccgc aaccccgccg cgctgcacaa
    56941 atggctgggc gaccaaatca gccttgccga aaacaacagc ggcgacaacc gcgccatccg
    57001 tctggaaagc gacgaagatt tggtcaaaat cgttaccatg cacgcctcga aaggtttgca
    57061 gtatccgctg gtgtactgcc cgtttgcgtg ggacgcgcaa gataccggac cgtccgactg
    57121 gcaaatcctc caccaaggcg caaaccgaac cgaactgttg gcaaaggcgc aactgtcgga
    57181 agacgaacag aaacaatacg ccgatgaaga aatggcggaa cgcctgcgcc tgctttatgt
    57241 cgcgctgacg cgtgccgagg aacagctcaa catctacgcc gcgtactcca ccaatactgc
    57301 cgacaacccc ctcgcctacc tgattgaagg ctcgccccaa gacagccgtg aaaccgtccg
    57361 ccgtgcctat gcgtgtgaaa aagacggcat cgcgatgctc aaacgcaact ggcggcgcgt
    57421 ggcggacaac gccccttccg gcacaaattt cgccttcacc gaaaatgcgc cgccccctgc
    57481 cgcctatcgc agcaacgccg gtcaaaccgc cgaatttgcc gcaaacagca ttcccgaacg
    57541 cggattccga tttgtccgcc acactagctt taccgcctta agccgccata cccaaacgcc
    57601 cgacggcggc gaagaagatg cctgcccgtc cttggatgcc gccgaaacct cggtgccggc
    57661 gatgccgtct gaaacgccga cggcttcaga cggcatatcg atacacgatt ttccgaaagg
    57721 cacgcaggcg gggctgtgcc tgcacgaaat tcttgaagat ttcaaattcg gacaagcagc
    57781 cgccggacag gaaaccctca ttgccgacaa gctgaaaaaa tacggttttg aagaaatatg
    57841 gctgcccgcc gttgccgaaa tggcggaagc ctgccgcaaa acgccgctga cgggggcata
    57901 cgacctgtcc gacatctcac ccgagtgccg ctgtcccgaa atgggcttta ccctccacac
    57961 cgaagacttc agcctcaaac gcctgcgcga ctggtttgcc cgcgacgaca tcaggctgcc
    58021 cgaagtctgc cgtgccgctg ccgaaacgct cgacttccac accgtcaacg gctttttaaa
    58081 cggctttgtc gatatggtct gccaagaccc cgacggcaat atctgcgtca tcgactacaa
    58141 atcaaaccac ctcggtacgg acgcatccgc ctacacgcaa caggcaatgg acgaagccgt
    58201 cgcacaccac cactattacc ttcaggcact gatttacgcc gttgccgccg cacgctactt
    58261 caagctgcgc ggacaaccgc ccgccgccgt ttccgtccgc tacctgtttt tgcgcggatt
    58321 ggatggcaaa ggcggcggcg tttggcgttg ggacatagat gccgccgctt tggaacagat
    58381 aaaataactt cccgtccttt cggacaacaa ccgcaggaaa accctatgaa tatcaaacac
    58441 ctgattaccg ccgcactcat tgcctcagcc gcctttgccg cgcaggcagc cccgcaaaaa
    58501 cccgtatccg ccgcccaaac cgcgcaacat ccggccgttt ggatcgatgt ccgttccgaa
    58561 caggaattta gcgaagggca tttgcacaac gcggtcaaca tccccgtcga ccaaatcgtc
    58621 cgccgcatac acgaagccgc gcccgacaaa gacacgccgg tcaacctcta ctgccgcagc
    58681 ggacggcgtg ccaaagccgc ccttcaggag ctgaaaaaag caggttatac aaatgttgcc
    58741 aatcacggcg gttatgaaga cctgctcaaa aaagggatga aatgacgcaa tgccgtctga
    58801 aaggacaggc tttcagacgg catacggcgt tccggcacgc ggcgcaacat cgggcgcggc
    58861 gcgcgccgcc ccgtccgaag cctgataaac aaaccgataa ggaacaatga tgtttcacga
    58921 cacataaagc accgccctat gtgttgccct gatttggaaa gggttacacc cctcccgaat
    58981 aaagtctgat tctaccgttt caaccaaaaa ggaaaaagga aatacgatga aacacatact
    59041 ccccctgatt gccgcatccg cactctgcat ttcaaccgct tcggcacatc ctgccagcga
    59101 accgtccacc caaaacgaaa ccgctatgac cacgcatacc ctcacctcaa aatacagttt
    59161 tgacgaaacc gtcagtcgcc ttgaaaccgc cataaaaagc aaagggatgg acatttttgc
    59221 cgtcatcgac catcaggaag ccgcccgccg aaacggctta acgatgcagc cggcaaaagt
    59281 catcgtcttc ggcacgccca aagccggcac gccgctgatg gtcaaagacc ccgccttcgc
    59341 cctgcaactg cccctacgcg tcctcgttac cgaaacggac ggcaaagtac gcgccgccta
    59401 taccgatacg cgcgccctca tcgccggcag ccgcatcggt ttcgacgaag tggcaaacac
    59461 tttggcaaac gccgaaaaac tgatacaaaa aaccgtaggc gaataagccc gtcgccgaca
    59521 tatggcggat ggaaatcaaa tgcacacgcc cctcattccc acgaagtcgg gaatccaatc
    59581 ggttgggtta ccgtgatttt caatcattgc cgaaacggac gattccggat tcccaccctt
    59641 gcggggatga cgggcgtgtg tgtttttgcc acatcacagg caactttgcc gcaaatctta
    59701 tctgtcgccg gtatgccttc ttgtttcaat ccgccacaaa ccaaatgccg tctgaaaccc
    59761 gatttcagac ggcatttccg tttattccct gctgtcgcgg caaatatcga tggcttcctg
    59821 caaactcgaa acgccgtaga ttttcaggtt cggaaactct ttggcgttgc gcggcatatt
    59881 ggctttgggg acgatggcgc gtttgaagcc gagtttttcc gcttctttga gccgctcttg
    59941 cccgcgtgcg acggggcgga cttcgccgct taagccgatt tcgccgaaaa ccacggtttt
    60001 ttcaggcata gggcggttgc ggaagctgga aagcatcgcg aggatgaccg ccaaatccgc
    60061 cgccggttcg ccgattttca cgccgccgac ggcgttgagg aacacatcct gatcgaaaca
    60121 ggcgatgccg ccgtggcggt ttaacacggc aagcagcatc gcaagacggt tttgttccag
    60181 tccgacggtg aggcgtttgg gcgtgaagcc gtgcgcgtca tcgaccaatg cctgaatttc
    60241 gaccaaaagc gggcggctgc cttcctgtgt aaccaaaacg cacgagccgg gcgtatcgtc
    60301 gcggtagctg gcgaggaaga tggcggacgg gttggacaca cctttcaaac cgttttccgt
    60361 catcgcgaac acgcccagtt cgtttgccgc gccgaagcgg tttttgatgg cgcgtatcat
    60421 gcggtagttg gaatgttggt cgccctcgaa atacagcacg gtatcaacca tgtgttccag
    60481 cacgcgcggg ccggcaatcg cgccgtcttt ggtcacgtgt ccgaccagta tcatggcgat
    60541 gcccatctgt ttcgccatgc gcgtcagttg ggcggcacac tcgcgcacct gcgacacgga
    60601 gccgggggcg gacgtgattt ggtcggaata catggtttgg atagagtcga tgacgacaac
    60661 ttcgggctga tgctgtttca aggccgcctg aatcgcttcc atgcggattt cggcaagaag
    60721 gtttacgccg tcggtcggca gttccaaacg ctgcgcgcgc agggcgactt gttgggcgga
    60781 ttcttcgccg gaaacgtata gcactttacg gctttgcgcc attttggcga tggtttgcaa
    60841 cagcagcgtg gatttgccga tgccggggtc gccgccgagc aggatgaccg caccatcgac
    60901 caaaccgccg cccaatacgc ggtcgagttc gcccataccg gtcggattgc gcggcacttc
    60961 ggtggcggta acggcggaga gggattggac ggtcgaagtg tccgccgccc aagattggaa
    61021 acgggcgttt ttcggctcgg gcgcggcaag gctttcctga agcgtgttcc actcgccgca
    61081 atgcgggcat ttgccttgcc atttcgggga ggtgccgccg cattcggtgc attggtaaag
    61141 ggttttaagc gtttttgcca tcggttttcc ctgcatctga aaacaaaaat gccgtctgaa
    61201 aatagggacg gttgggattg cgccaatacg acaacgccgc cgccgtcatg tactatccat
    61261 acacggcgga tattgccgca ttgccttatt tttaccctaa ccggccatct ctcggcttca
    61321 gacggcatat cgggcggttt cctgccggct tacccgccgt gatactgccg agacagctcg
    61381 tgtacggtat cgactaagat tttggcgtgt tcggggtcgg cgtgttggtt gatgccgtgt
    61441 ccgagattga agacgtggcc gctgccgtgt ccgtagtcgg ctaggatgcg cgcgacttcg
    61501 gtgcggatgg attcgggtgt gccaaagaga gcaaacgggt cgaagttgcc ttgcagggcg
    61561 acttgcttgc cgacgcggcg gcgtgcttcg ccgatgttgc acgtccagtc caagcccaat
    61621 gcgtctgcgc cgatttctgc catgctttcc agccacagcc cgccgccttt ggcaaacacg
    61681 atgacaggca cgcgcctgcc ttcgctttcg cgtttcagtc cggcaacaat ttggcggatg
    61741 tatttgaggc tgaattcttt aaacgccgca tcgctcaaga cgccgcccca agtgtcgaaa
    61801 atctgcacag cctgcgctcc cgcgtcgatt tgggcgttga ggtaggcggt aacggcttgg
    61861 gcgttggtat cgaggatttt gtgcagcaaa tcggggcgcg agtacatcat ggttttgatg
    61921 gtgcggaatt ctttgctgct gccgccttcg accatgtaac aggcgagcgt gaacggactg
    61981 ccggagaagc cgataagcgg tacgcggccg tccaatgctt tacggatgga agttaccgcg
    62041 tcgaaaacgt attgcagttt ttccatatcg gggacgtgca atttggcaat gtcgtcctcg
    62101 tgttgcaggg cgcgtttgaa tttcgggcct tcgccttcgg caaaatacag tcccaagccc
    62161 attgcgtcgg ggacggtcag gatgtcggaa aacaaaatcg ccgcgtccaa atcgaaacgt
    62221 tccaaaggtt ggatggtaac ttcggtcgcc aattcggtgt ttttgcacaa atcgaggaag
    62281 ctgcccgctt tcgcgcgtgt ggctttgtat tcgggcagat aacgccccgc ctgacgcatc
    62341 atccaaatcg gcgtgtattc gacgggctgt ttaagcaggg cgcggaggaa ggtatcgttt
    62401 tttaaaagag tcatacgggt tttctgtcag tctgtttgtc aagtgaaatg gttttcagac
    62461 ggcatttcaa cgatgccgtc tgaaggtttc aatgggtttc ggcggaaggt cggttttcct
    62521 cggcaacgct tgccaaaacc aagttgcgct gcgcctccgc cttctgcggt tctccggttt
    62581 cgtcaaaaac ctttgccaga accaaacgcg cggaaatact cggctttaat gcaatgctcg
    62641 cttcaaggta gccttttgcc ttgccccaaa gtttgcggcc gtaggcgagc cgaccgagat
    62701 acatcagcag aagcgcgtta tcgggctgtt ctttcagcca agcatcggca aaatcgatgg
    62761 ctttctgctg ttcgcgttcg cccaaaaagc gcacgctttc gacaaaggct tccaaaagct
    62821 cggggcggcg gttgtgcgga taatgctgtt tgacccattt gaccgcatcg gcatacagtc
    62881 ccaaacgttc gtacttttcc gcaaccgata cgctcaattc cccgtttttg aggctgtcgg
    62941 gaatccgctt caggcaggtt ttcaaagcgg cggcatcggc agcatccgcc agctggcggc
    63001 ggtatgccca attttgatac cgttccattt ccgatttgcc caacgcgccc gccttggaaa
    63061 gtttttcggt ttttgccaga acctgcaacg cgtcgcccct gtcgaaagcg taacgaagtt
    63121 gcagacgcac gaggcgcgta aggttggcat tcatcttcgc cgccgcatga agattggctt
    63181 ccgccgcttc gtaatcgcgc cggttcaacg ccgattccgc caacaaaaga taacgggaaa
    63241 gctgctgctt ttccggcagt ttggcgattt ccgcaagata acggtcgcgc agctcgatgt
    63301 tttccatctg cccggcggca tgtgcgccca acatcaatgc caaagtccgg ttgtctccgg
    63361 cctctttgtt gaccaacacg cgtgaggctt ctagttccgc cttttcaaaa cgcccttcaa
    63421 aatacgccaa acccgccttg ttcaaggcaa gcgcggcctt gcggccttta cgcgccgaac
    63481 cgaaacgctg catcttttcg gggatattga atacgccgat aatgaattta aacaggaaat
    63541 accacaccac gacggcaatc agcgaaccta acacaaaggc gtgcaggttg attctgagca
    63601 tggtctgtcc gagtacgata tacacgtcgc cggtgtaaat gcccgaagcc aatgccagcc
    63661 cgacggcggc ggcaaacagg acgacaatcc agactaccgt tttcatgcgc gttctccttt
    63721 agcggacggc gaacgccctg ccttttccgg tacgggctgt ttttttgcag gctgttccgg
    63781 tttgcgttcc gaaggcaggg acggtgcttc tatggtcttc acatccgatg cggcagccgg
    63841 ttcggaagct gtgttttcgt ttgccggttc ggaagccgcc tgttctgctt cttgattttc
    63901 cgccgccgtc atgcgcgtac cgtcgcgata ggcgcggacg gcatttaggc tgtttttcaa
    63961 accgtcatcc gccgtcatgc gcacatccaa cgccttcaat tccgccagtt ctttcagcca
    64021 cgactgcgtg gcgggagact tggcatcgaa atactgtctg acggcggctt cggcattgtt
    64081 caaatcgccc tgatagactt cgctgttgcg ctgcattaat gcagtccgcg catccaaaag
    64141 gcggaggcgc aggttttcac gcacaaaata tgcctgttcg ggagaaatca gcatggcatc
    64201 gttgttttcc aaacggcgga tttcgaccag ccccttcaat gtgccgaggg atttttccca
    64261 tacgttctgc caccatgaag cagaagcggc ttcgttcttc acctgtacgc ccggcttcaa
    64321 tacgccgtcc aaaatcagcg gcagtccgga tacggcggtt tccagcctgt cgaggcgcaa
    64381 tgccgtgccg gaaatatcga catagggacg gtttttcagt tccgccaagt cgctgctgac
    64441 tgcctgtttg atcggcagaa gctctgcctg atcgaaacgg gacaggcggc tgtcgatatg
    64501 ctccaatacg ccgactgccg tttggatatt gccggtcaac accagctgtt gcgccgccag
    64561 attgagtatg gtctcggttt cgtccaccag ccaatcggcg cgtcctttgg tcaactcgcg
    64621 ataggatttt tgcatttcca agatttgttc gccgtttgct ttgacgtttc cgtccaaacg
    64681 gtcgagctct gattgtatgg cggcttgccg gttgaggttg tctttcaaca gggcggcgtt
    64741 ttccgactcg cccaaggcgg ctttgtcgat tttttggttg aatgccagct cttggttttt
    64801 caagacattc tgtccttgga caaacaaaaa accacttgta cccaaaccca atgccgccaa
    64861 taccagggcg cagactgcca aagcgttgct gccggactgt ttgacgataa aaggagcggc
    64921 aggaatgtgt acttctgttt cgttttcttt gcgtggggaa gaggtttcag acggcatttc
    64981 ttttgatgcc tgtatctcgc gtacgggttc ggatgatttg ttttcaggtt cgcccaccgt
    65041 tttgctcctt attaatttga ggttccggga aagaccatgc cgtctgaaac ggaaatggaa
    65101 gaatgggaaa gcgcggcttc cagcgtaggg acggtttcga ccgaacacac gccttcgcgc
    65161 ttcaatgcct ccgcaatgcg cggatgatgg gtaaagtata gcaaggattt gaagaatcgg
    65221 gaaaattgcg gcggaagctg tccgaacagc aagcgcacga gttctgtgga cgtaatatag
    65281 gcggcggcaa tattttcggt ttggaaattt tgaaagttca aaggtttatg ccgtctgaaa
    65341 tagacttctg ccacctccgt ccgaaaacct ttctcctgca aggcattcat cagaaaatcc
    65401 cgcccgccgt gtccgcgcac aaacaatacg cgcgcacctt cgggcagact gttccaaacc
    65461 gtcaggcgca aaaccgcctc gctgtcgttg ccgtcttcag gcgcgatgac cgttctgccc
    65521 aaacagcgtt ccaatacgcg gcggctgccc tgccctacgg aaatgtgcat ctttatgccg
    65581 tctgaaaggt taaggtacgg gacggcggtt tcaacggcgg tcggactgac ccaaaacacg
    65641 gcatccgcac gggcatattg ttcggacaaa agttccagtc cggcagcatc tgcttcgatt
    65701 tcaatcgggc tcaacacttc cgcctgccat ccggcacgac ggcaggtttc cacatcgtcc
    65761 ttcgccctgc ccgacggtcg gacaatcagc atgatgggtt tcactgtttc catcgcctta
    65821 tccgccccga ttaccgggaa aaatcagcat cgttgaaatt cgcctgcgcg gtttgcggcg
    65881 tacagccaac ccgtgcgact tccttgcccc tacgctccac aacaacaccg ctgctttccg
    65941 tatgagtcac ggaatcgaag cccgtccata cggtgtacat aaattcgccg ttttggaatt
    66001 tcatcgttgc ccggcgtccg ctacccatgc cttgccacct gtcggaacgc cccaacaggt
    66061 cagctttgct gttgcgtatg gcaatttctt tttttgccgc actgccgaac gaatattcgt
    66121 aaagattgcg gttgattttt tgaacttcta tatatttgtt gttgtccgtt ttacaggaaa
    66181 acaccgtatc cgcccacacc gtttgggcgg cagccgacag aagcaataca gcagaaagga
    66241 acattttttt catcgtattt ccttaatggt tgaaactccg ccatttggac atccgtcctt
    66301 cgtggcagca gcatcagatg ttatttgaga ggggtgtaac cccttccaaa ttagggcaac
    66361 acatagggca acgctttatg tgtcgtcctg tgtgttgaaa catcattgcc cgtatcctga
    66421 aatagaaaag aaaatgcccg ttttacggtc ttctttccga caaaatgccg tctgaaacgg
    66481 atttcagacg gcatggtcga aaattattgc gcggcaagcg gcttgagcag caaggtttcg
    66541 ccgctttgca caactttttc aagcaggtcg atattgggat gcttgccggg atgggcgcgc
    66601 gcgtcggcaa cgtgttcggc aaaccatatc aaaccctgtt ctgccgcttc tttgtccaat
    66661 tttccgtcaa aacgttctgc caaagcgttg tacaacttga gcgaaccgag cttaccctga
    66721 acggcaggaa tgtggtgaac cgttttgcca tctgcatcct gaacgtccaa accgcccaga
    66781 tggtcgattg aaggaaatgt ggcgagatag tcttgaaaat tcatatcaac tccgaaaatc
    66841 agtcagttag cgcgtgccga aaatcttgtc gcccgcatcg cccaagccgg ggatgatgta
    66901 gccgttctcg ttcaagtggc tgtcgagcgc ggcggtgtaa atcgtaacgt cgggatgcgc
    66961 gtcgttgacc gccttcacac cctcgggcgc ggcaaccagc accagtgcct tgatgttttt
    67021 gcagcccttg gctttcaaaa ggtcgatggt ggcaaccatc gaaccgcccg tcgccagcat
    67081 cgggtcgata atcagggcag gacgttcgtc catgctgtcc acgaatttct cgaagtagga
    67141 aacgggtttc agcgtttcct cgtcgcgctg cagtccgact acgctgattt tggcagtcgg
    67201 aatcaggtcg agtacgccgt caagcatacc caaacctgca cgcaggatgg gaacgacggt
    67261 caatgttttg cccttgatgc ggtcgccttc aatctgaccg caccatccgt cgataaggta
    67321 tttttcgatt tcaaaatcac ggcttgcctc gtatgccatc aggcgcgcca gctcgatggc
    67381 aagcgtccgg aatttgtagg tgctgcaatc cgcctccctc atcagggtta atttgtggcg
    67441 gacgagcgga tggttgataa cattaacgtt catcgcggtg ttccagtgtg attttgagac
    67501 gcgccattat actttttttt gatttgccgg aaaagaaaat gccgtctgaa acacgttcag
    67561 acggcattgc cgaatccatc aggctttcag cagctcttgc agctcgcctg cctcatacat
    67621 ttccatcagg atgtccgaac cgccgacaaa ctcgccgttc acataaagtt gggggatggt
    67681 cggccagtcg ctgtattcct taatgccttg gcgtacttcg ggattttcca atacgttgac
    67741 ggtaacgtaa tcggtgcagc ctgccgcgtt caggatttgc acggcgcggg aagagaaacc
    67801 gcattgcgga aactgcttcg tacctttcat aaacaatacg acgcggtgtg tcgttactac
    67861 ttctttaatt tggtcgtgga tggaagccat cgcttattcc ttttgataaa gttgacaaaa
    67921 tttgtcaggt aggacgcatt atacgcaaaa gcggaaactt cccacaatat ttgccgtata
    67981 tcgcgcagta tcaatgttgt gaacggaaat attcccactc ctcgacaatc ctgctgtccg
    68041 gcaaatggca cagggcatat ccgccgccgc tttcgtcttt ttttacttcc cgtctgccgc
    68101 ctttggcaat gcagaatttg gaagcggaat ttgccatgcc tgtccgaaac gtgttggcag
    68161 gcagccggca gcagcgcgga catgcatacg gatgaattgc cttcatggtt ttccttatcg
    68221 tcggtacagg gtcagacggt ccgcatatcg ggtacttttt taaacagccg gtaaaagttt
    68281 tccgtcgtat atgccgcaac ctgttccaat gtttggttcc gcaatttggc gatatgttcg
    68341 gcggtatggc gcacaaaagc cggttcgttc tgcctgccgc gtttgggaac gggggcgagg
    68401 aacggcgcat cggtttccac caaaatgcgg tcgtccggaa cgtattttgc cgcctcctga
    68461 accaagggtg cgtttttaaa ggtaacgatt cccgagaaag aaatataaag ccccaaatcc
    68521 attgctgcac gggcaaaacc gatgtcttcg gaaaaacagt ggataacgcc cgaattaacc
    68581 cggcattctt tcaggataga caaggtgtcc gccgccgcat cacgcgtatg gacgataacg
    68641 ggcagtccgg tttgattggc tgcttcgatg tggtctgcaa agcgtttgtg ttgccaggac
    68701 aaatcgcctt tgcaccagta ataatccaaa cccgtctcgc caatgccgac cactttcggc
    68761 gaggcggctg cggcaaccat ttccgcaata gaaaattctt cggcttcctt gctgtcggga
    68821 tgtacgccta tggtgcaata gatgtgttcg tgcgcttcgg cgatggcaaa gacttcggag
    68881 aagctttccc tactgacgct gatggcgagc gcctgcccca cgccgtttgc ttccatgttg
    68941 gacaaaactt cgggcaggcg ttctttcaaa ccttcaaaat tgaggtggca gtgcgaatcg
    69001 atgatatgca tggcgttaca tggtaaacgt aggtctgctg ccgccgattg tgttgcccag
    69061 ttcgacttcg atctggtctt tgactttcag gcagttttcg tgttttgtga atgccagccc
    69121 cacccctttg ggtttggagg aagtacgcgc aggattgatc caggcgactt tggtcggcag
    69181 gaacagtttg gggaagttga ggatttctac ggcaagcaga atatcgtccc cgatggaaaa
    69241 tacgtcgttg gtctgcacaa acagaccgcc gtgttccaaa aacggcatgt aggagctgta
    69301 cagcagattc atgtctttca gctgcaacga catcattttt gccggaatat tttgtccgtc
    69361 tgacataatt cacccgttct ctttcttcaa ttccaaatag ttgatgagca gatgctcgat
    69421 ctgcattttg acatttaaag tatgaaaccc gtagggggca agctgtttga gcatatcctc
    69481 cgccgcaaat acattgcgca ggcggaaacc ggataccgtc tgaagcagcc tgtcttcata
    69541 agcgggataa tagacgggtt tcatgtgttg caggcacaat cccaaatcga ccagccattt
    69601 ctgcatccac ccgacaaata cggcgagcgg aagtttttcc ttatcgaaaa gcgcggcgta
    69661 atccaaaatc ttcaacaacc ttggttctgc caaaatatcc aacagtttaa tccgcaacgc
    69721 acggacaccg tccgcctcat caaacagcgg cgctccggaa tggaaagcca gacgttcctc
    69781 aggttccgcc acaccccttt cacgcagata tgccaatgcc tcttcatggg aaggcgcggg
    69841 caaaaccatc ttccggcagc ggcttttaat ggtcggtaaa accttgtccg ccgcgtggct
    69901 gaccagcaaa aagaccactt gtggcggcgg ctcttccaac actttcaaca aactgttggc
    69961 ggcttggaca ttcatacttt ccgcaggatg aatcagaatc acgcgcaaac cgccccgtac
    70021 cgaagtcagg tacacattat cgatgatttc cctgacggca tcgattttga tctgcaacag
    70081 tttgcgtccg ttttcgggtt cgtccgacaa gggggtgatt tcgtaaaaat cgggatggct
    70141 tccctgtcca aacagatggc aggacataca ttcgccacag ggtttgcagc ccggtgcagg
    70201 ggtttcgcac aacagtgcct tcgccgcaaa gcgggcaaat gtagttttcc ccgtatcttt
    70261 tttgccggca aacagccatg cattgggacg acgctcccaa tgttccgcaa tctgccgcca
    70321 ttgctcatta tgccacggat aaatcatttc aaaccatccg tccggaaatc cataccgcca
    70381 tcagagcagt tccaaatcgg ggctggaact ttgcgcgcgg cggctgcgca actgtaccgc
    70441 caaacgcaaa tcgtgtgcgg aatcggcatt tttcaatgct tcttgcaggg aaatatcgcc
    70501 tttttcatac aattggtaaa ggtgttgatc gaaggtctgc atacccaggg tagtggattt
    70561 tttcatcact tctttgattt catggatgtt gccgttgtga atcaactccg aaatcagggg
    70621 cgaattgagc agcacctcga ctgccgccac cctgcccttg ccgccgtctc gcggaacgag
    70681 gcgttgcgaa ataaacgcct gaaggttgag cgacaaatcc gtcagcaatt gttcgcgccg
    70741 ctcctcgggg aaaaagttga tgatgcggtc gagtgcctga ttggtgctgt tggcgtgcag
    70801 cgtcgccata cacaaatgcc ccgtttcggc aaaggcaatg gcgtagtcca ttgtttcgcg
    70861 gtcgcggatt tcgccgataa ggatgacatc cggcgcctga cgcagcgtgt ttttcaacgc
    70921 cgccatccag ttttccgtat ccacgccgac ctcgcgctgg gtgatgatgc agtttttgtg
    70981 ttcgtggaca aactcgatcg ggtcttcgat ggtgatgatg tgtccgaacg aattttcatt
    71041 gcggtaatcg ataagcgagg caagcgaagt cgatttgccc gagccggtgc cgccgacaaa
    71101 aataaccagc ccgcgttttt tcagcgcgac atccttcaag accggcggca ggttcaggct
    71161 ttcaaacttg ggaatcttgc tggtaatcgc acggaatacc aacgccgtcg cgccgcgctg
    71221 tatcatcgca ttgacgcgga agcggctggt gtccggcagg ctgatggcga agttgcactc
    71281 gttggtcgat gaaaattctt ccgcctgctt cgcactcata atcgaaaagg cgatttccat
    71341 acatttttcc gccgtcagcg gttcgtccgt gatgcgggtg attttgccgt ccagcttcat
    71401 cgcgggcggg aaatgggtcg tcacaaacag gtcggaacct ttgtttttgt tcatatggcg
    71461 cagccatgcg aacagttctt cctttgcggt catattttca tcggtaaaca tcctcgtccc
    71521 ctaatcttca aacaatctgt accttcccat tctaccaaaa acccgccgtg ccgtctgaaa
    71581 ccgtttcaga cggcacggac aaaccacctc cactcaatcc tgccgtgtta gcgtgtataa
    71641 ttcggcattc tatccgaaat tcacagcagg tcagcaatat gtctttgaaa acagtagccg
    71701 ttatcggcgc aatggaacag gaaatcaagc ttttgcgcga gatgatggaa aatatcaaag
    71761 ccgtctcttt cggcagattt tccgcctatg aaggcgaatt ggcgggaaaa cgcatggtgc
    71821 ttgcattgag cggcatcggc aaggtcaacg cggcggttgc aacggcttgg attatccgtg
    71881 aattcgcagc ggactgcgtc atcaacaccg gcagcgcggg cggtttgggc aagggtttga
    71941 aagtcggcga cgtagtcatc ggcacggaaa ccgcacacca cgatgtcgac gtaaccgcat
    72001 tcggctatgt ttggggacaa gtgccgcaac tgcccgccgt atttgtttca gacggcctct
    72061 tggtcggcaa agcaaaacag gcggcggaag tgtttgaagg cgcggcggta gaacaaggcc
    72121 tgattgtcag cggcgaccgc ttcgtccaca gcagcgaagg cgtggcggaa atccgcaagc
    72181 acttccccaa agtcaaagca gtggaaatgg aagcggcggc gattgcccaa acctgtcatc
    72241 agttggaaac gcctttcgtc atcatccgcg cggtttccga ttcggcggac gaaaaggcag
    72301 acatcagctt tgacgaattt ttgaaaacgg cggcggcaag ttctgcaaaa atggtggcag
    72361 aaatcgtcaa atctttataa aacctttatc aaatcatgcc aaaacagcgg ttttccgcta
    72421 gaatgtcggg ctgtttatcc cgttgcgccc gacattcgga cgcagcgccg tttgttcaga
    72481 cggcatttcc aaaacccact gtccgacatt caaaataact ctaatgaaaa atatccgaaa
    72541 tttctccatc attgcccaca tcgaccacgg caaatcgacg cttgccgacc gttttatcca
    72601 atactgcggc ggcttggatt tgcgcgaaat gagtacgcag gtactcgatt ctatggacat
    72661 cgaaaaagag cgcggcatca ccatcaaagc gcaaaccgcc gcgctcaact acaaagcgcg
    72721 cgacgggcag gtgtatcagc tcaacctgat tgacacgccg ggacacgtcg acttttctta
    72781 cgaagtttcc cgttcgctgt cggcctgcga aggcgcgctt ttggtggttg acgcatctca
    72841 aggcgtggaa gcgcaaaccg tggcgaactg ctacaccgcg attgatttgg gcgttgaagt
    72901 cgtgcccgtt ttgaacaaaa tcgacctgcc cgccgccgac cccgaacgcg tggaacagga
    72961 aatcgaagat atcatcggca tcgatgccgt cggcgcggtg caatgttccg ccaaaagcgg
    73021 catcggcgtg gaagacgttt tggaagaaat cgttgccaaa atccccgcac cggccggcga
    73081 tgaaaatgcg ccgctgcaag cggtgattgt cgattcgtgg tttgacaact atgtcggcgt
    73141 ggttatgctg attcgtgtga aaaacggcac catcaagctg aaagacaaag tgcgctttat
    73201 gagcaccaag gcggaaacgc aggttgagca gctgggcgta ttcacgccga aatcagttca
    73261 aaaacaagaa ctcaaagccg gcgaagtggg ctttttgatt accggcgtaa aagaattggg
    73321 acaggcaaaa gtcggcgata cagttacttt gattgccaac cccgcctccg agccgctgcc
    73381 cggcttccaa gaagtgcaaa gccaggtatt cgccggactt tatccggtgg aaagccacga
    73441 ctacgaagcc ttgcgcgacg ctttggaaaa attgcagctt aacgatgctt cgttgaaatt
    73501 cgagcctgaa gtttcccaag cattgggttt cggcttccgt tgcggcttct tgggcctgtt
    73561 gcatttggaa atcgtgcagg aacgtttgga acgcgagttc gacatggact tgattaccac
    73621 cgcgccgacg gtggtttatg aagtcgtgtt gaaaaacggc gagaaaatcg aagtcgaaaa
    73681 tccgtccaaa ctgcccgaca tcggcagcat cgaaaccatt ctcgagccga ttatcactgc
    73741 aaccatcctc gtgccgcagg aatatgtcgg caacgtcatg actttgtgca accaaaagcg
    73801 cggcgtgcaa gtcaatatgc aatatatggg ccgccaagtg atgctgactt acgacttgcc
    73861 gatgaacgaa gtggtgatgg actttttcga caaactcaaa tctacttcgc gcggctatgc
    73921 ttcgttggac taccatttca aagagttcca accgtctgat ttgattaagc tggatatcat
    73981 ggtcaacggt gaaaaagtcg atgcgctgag cctgattgtc caccgtcaaa gcgcggttca
    74041 ccggggccgc gaactggcat cgaaaatgcg cgagctgatt ccgcgccaaa tgtttgatat
    74101 tgccgtccaa gccgccatcg gcagtcagat tatcgcccgt gagaacgtca aagccctgcg
    74161 taaaaacgtt ttggcaaaat gttacggcgg cgatattacg cgtaagaaaa aactgcttga
    74221 aaaacaaaaa gcaggcaaac gccgtatgaa acaagtgggc aatgtggaaa tcccgcaaag
    74281 cgcgttcttg gcaattttgc aggtgagcga caaataatat ccggcacacg gggcagtgca
    74341 tcatgcccct taaaccgccc tgattgtaag agagcacgtc cgccaacaaa ctttgtgatt
    74401 taccgcctct taaaaacggt tgcacggacg ggcgggtttt taaccgttaa ggaacaacaa
    74461 tgaacattat gctaatgtcg ggcgcggctg ccgcgctgct tgccggcatc atcctttatt
    74521 tcaaaagcga caaaaagcgg caggaaaacg gtgaatggag ttccggtctg gaatacgcct
    74581 atatcctgac ggcggtcggc gtgtttgccg ctttgtccct gtttatgagc tttaccgccg
    74641 ttttcctgat tttcgttgta ttgtgcggta cggcttgggg ggtatacaaa taccgcctga
    74701 agactcatcc cgaaatctcg gaaagcagcc acttcggcga ttatttcggc agtttcttcc
    74761 ctaccgtttt ggtattgttc ctcatccggt cgtttattgc cgaaccgttc caaatcccgt
    74821 ccagctcgat gcgcccgggc ctgattaagg gcgatttcat tttggtcggc aaattttcct
    74881 acggcctgcg cgtacccgtt ttaaacaatg tattcatccc cacgggcaaa atcgaacggg
    74941 gcgatgtggt cgtttttaat tatcctttgc aaccggagat gacctacatc aagcgtattg
    75001 tcggcattcc gggcgatgtg gtcgaatatc ggaataaagt tttgacggta aacggaaaac
    75061 ctgcttccga tattcctgac ggcacatacc gttatcccga cgacaccgac ccatccgaaa
    75121 tccacaatac ggatatgttc cacagcggtt tggacggcaa atccttcaat attctgaaaa
    75181 aagaaggaca gcctgccgtt tccctgcccg tattgggcaa atatacctcc gatattatgt
    75241 ctgaaaacgg atattccata gagcaaagcg gtttggaaca ctgccaatat gccgacgacg
    75301 gcagcggttt cgtgtgcaaa gttcccgaag gacgctattt cgctatgggc gacaaccgcg
    75361 acaacagtgc cgattcgcgc tactggggat ttgtggatga caagctggtt gtcggcaagg
    75421 caatgttcat tttgatgaac ttcggcgatt tcggcagggc cggtacggca atccgttagg
    75481 ccaggaagcc aagaatgccg tctgaaaata gtttcagacg gcatttgcta ttcctcactt
    75541 ttgcaggcgg atctaacgtt gtcggatttt cctacctata gtgaattaaa tttaaatcag
    75601 gacaaggcgg cgagccgcag acagtacaaa tagtacggca aggcgagcca acgctgtacc
    75661 ggtttaaatt taattcacta tatcagtccg gtaacgcagg ttttgcacgg aaaactgcca
    75721 atactgcctt gatggaatca atattaagac tgctgtttca gcgaaccgag gtaaattgcc
    75781 gaaagcgtca gcgccacccc tgcccactgc acgccttcaa tcggtttgcc caacccgaaa
    75841 taatcgatga acagggcggc aaccggttcg gacaaaagca gcagccccgt cagcgacagc
    75901 gaaagcagcg gaatcgcata ggcaaccatc gcccacgcga agcactgcat caccacgccg
    75961 tacacaagca ccaaacccgc atctttccaa gtcgtcggat aaagcacgcc gccatccatc
    76021 agcaatgccg gaacaaccag cgataccgcg ccgcccaaac tcaaaatcat cattgaaggg
    76081 aaaagcgcca ccggctcgat ttcatgggtt ttgcggacga acaccatcga cagggcgagc
    76141 atcagtcccg ataccaaacc gctggcgaat ccccaaaccg cattaccgtt gtagccgaat
    76201 tccgcaccgg caatcatcgc cacgccggca actgccgata ttaagcctgc ctttttcagc
    76261 ccgctcaaac gctcgccgaa aaagaaaaca ccgattgccg acaagaaaaa gatttgcagg
    76321 ctgttgagca gggtggaaat acccggcccg accgcgtgta tgctttcgtg ccacaacgcc
    76381 aaatcgaaag cgagaaacac gcccgccgtc agggcatatc ggacggtttt cctgtttttt
    76441 gggaattttt gcccgaaaaa ccgtgctaaa aaccaaaata cgaacaccga aatcagcaac
    76501 cgccaaaatg cgattgcata cgaaccgacg gggacggatc tgacaatcag actgcccaga
    76561 ccgaacacca cgcaacccag caccagcaat ggtgcggcat attttttgcc gttaccgacc
    76621 acaatatccc ctcccttccc catacggctg ccgcgccgac ggcaagaatg ccgccattcc
    76681 gaaaatatgc cgtctgaagc ccaaagcccc ggctgcaccc ggacaaacgg ctgattttac
    76741 gggcaggcag gctattttcc gttggggcaa aaaaccgtcc tgccggataa aacccgaata
    76801 tagtggatta acaaaaacca gtacagcgtt gcctcgcctt agctcaaaga gaacgattct
    76861 ctaaggtgct gaagcaccaa gtgaatcggt tccgtactat agtggattaa caaaaaccag
    76921 tacagcgttg cctcgccttg ccgtactatc tgtactgtct gcggcttcat tgccttgtcc
    76981 tgatttttgt taatccacta tatctgtact gtctgcggct tcgtcgcctt gtcctgattt
    77041 ttgttaatcc actataaaaa atatttccct caagcgatgt ggcggcacca acacctgatt
    77101 aaaacacaac cggcgttttc ggcatatacg caaaaacgca ttttccggca cggcgttaca
    77161 ccgataccga aaaatgcgtc tgaatgtttc gacaatccga ttaaagcatt atgccaaatc
    77221 tgcaaaaagt ggcgtagaga gatagcgttc gccataagaa ggcagcagca cgactatcag
    77281 cttgccttcg ttttcaggct gtttggcaag ctgcaacgca ctccaaaccg ccgcaccgga
    77341 agaaataccc accaaaatgc cttctttttc cgctattgcg cgggcggttt caaaagccgc
    77401 ttcgttcgac actttggtaa tgctgtcgta aattttggta ttcaaaacgg tcggaataaa
    77461 gcctgcgccg atgccttgaa tcgggtgcgg gcctttttcg ccgccgctca atacgggtga
    77521 agcttcaggc tcgacggcaa ccactttaac ttcgggtttg tattttttca acacttcgcc
    77581 cacgccggta atcgtaccgc ccgtgccgac gccggcaacg aagacatcga ctttgccgtc
    77641 cgtatcccgc caaatttcct cggcggttgt tttgcggtgg acttcggggt ttgcctcatt
    77701 gtcgaactgg cgcggcataa aataagtgtc gggatgcgcg tccaccaagg atttcgcttt
    77761 ggcaatcgcg cccgccatac cttcggcggc aggggtcaga atcagctccg caccaaacgc
    77821 gcgcaacagc attttgcgct ccttactcat gctttccggc atggtaatcg ccagcttgta
    77881 gccgcgtgcg gcacatacca ttgccaaacc gacacccgta ttgccgctgg ttgcttcgac
    77941 aatgacggtg tttttgttga ttttgcccgc tttttcggca ccctcgatca ttgcttcggc
    78001 aatgcggtct ttgacgctgc tgcctggatt gaaaaattcc agtttcacgg caacctccgc
    78061 cttcaaacct tcggtcagac ggttcagttt gaccaaaggc gtgttgccga ttagttcggt
    78121 gatgctgttt gcaattttca tcttctgctc ctgaataata aggtttcgac agtaaggcgc
    78181 aatccccata ggcatcctgt ccgattgcag gggatttgcg ccgaaacata aacaaatttc
    78241 ggatatggct ttatcctaaa cctatgccgc ctaaaaaacc aatatccaaa atttctttct
    78301 ttataacttt ttgttccgat acggccgcac cgtattatct tccaatttct gaaagcagca
    78361 gcaccagtac cgagacctgc atcaacgctg ccgtcaaccc gccgagtcgc agctgcgagc
    78421 cgccgaacgt tgacggcggc tgccggtcta taccgcagcg gtggacatag gcaaccgcca
    78481 tcagcacaaa ctgcaacacg aaccaataaa ccggcttcag cgcataaagt ttgagcagtg
    78541 ccgcaaacac aaacacttga accgttcccg aaaagcaata atgcaccaac gcacatattc
    78601 cgacaaaagc aagcgtcatc gatacattac tgacggcaaa cagcccgagc agaggatgtt
    78661 cggggtctgc ctgccatccg tttccatccg ttttccggtt ccaatgcccg atgaaaaaaa
    78721 atatgctgcc caaagtcagg taaaaatggg ggatgaacaa gggcgcggcg cgggtcattc
    78781 cccatatgcc gggcatcagc tttgccccca aaaccgatat gcccagccac agcataatac
    78841 tcgcccaaaa ccattgcaac attcctgcgc ggaatatcag aaacagaaaa accgccgcat
    78901 acagtaaggc agacaaaaaa tccaaatgca gcaaaatcat gaatacgcca actcgccttc
    78961 aaacaccgtt tccgcagggc cggtcatcat cacatcgccg ccgcaggccc attcgatata
    79021 taaagttccg cccggcaaaa tcacctctac cgttttccct tcatccaaca accccagacg
    79081 gatacccgcc accacagccg cacacgcgcc cgtaccgcac gcttgggttt cgcccacgcc
    79141 gcgctcgaac acgcgcaaac gaatcgcggt tcggctgata atctgcataa agccgacgtt
    79201 gacgcgttcg ggaaactgcc tgtgcggttc gataagcgaa ccggtttcgc gcaccggcgc
    79261 gcattctacg tcatcgacca caatcaccgc atgggggttg cccatattga cgcagctgac
    79321 aggctgaatg ccggattcga gatgcacccc gtaaatacag gcatcatccc cctcgcccga
    79381 ttcggggaca aacggtattt cagacggcat aaacctcggt ttgcccatat tgaccgtaac
    79441 catgccgtta tcggacaatt tcggaaaaat aacgccattt gccgtttcaa cacaaatttc
    79501 tttcttatcg gtcaaaccct tgtctgcaac aaaacgggca aaacaacgcg ctccgttgcc
    79561 gcattgctcg acctcgctac cgtcggcatt gaaaatacgg taacggaaat ccacgccttc
    79621 ggtttccgaa cgcccgacca ccaaaagctg gtcgaagccc acgccccgga agcggtccgc
    79681 ccatgccgca atcggcgcgt cctcgggggt aaaatcctga ctgaccgcgt caatcaccat
    79741 aaaatcgttg cccaaaccgt gcattttggt aaatttcaaa gtgttcatat tgttccgaaa
    79801 tcccgtatcg tctgttgctt ccgaatccga aatcccccgc accgaaggga aatgcttcca
    79861 tctcccgccg cctgaggatt aaaatcagcc attatactgt ttgttacccc ttacaagaaa
    79921 caccatgttc cgcatattac tgttgctttt cccttgtgca atccttgccg cctgcgcccg
    79981 aaacataccg gcaagccccg ccgtttcaaa ttgcgcgcat cttcaaaacc gcaccatcct
    80041 gtattttgac gcacacggca aacccgccga cggcgcagta aagggcggat tttacaaaat
    80101 tatcgaaata cagggtgcaa actgcatcct tattcaagac tttaacagca cgggcaatgc
    80161 cctcggcgga tcctatgtgc cggactgccg caaatcatcc cttttcccga aaaatatttg
    80221 acttcaaact gcctatgaac cacgacatca ctttcctcac cctgttccta ctcggtttct
    80281 tcggcggaac gcactgcatc ggtatgtgcg gcggattaag cagcgcgttt gcgctccaac
    80341 tccccccgca tatcaaccga ttttggctga tcctgctgct taacacagga cgggtaagca
    80401 gctatacggc aatcggcctg atactcggat taatcggaca ggtcggcgtt tcactcgacc
    80461 aaacccgcgt cctgcagaat attttataca cggccgccaa cctcctgctg ctctttttgg
    80521 gcttatactt gagcggtatt tcttccttgg cggcaaaaat cgagaaaatc ggcaaaccga
    80581 tatggcggaa cctgaacccg atactcaacc ggctgttacc cataaaatcc atacccgcct
    80641 gccttgcggt cggaatatta tggggctggc tgccgtgcgg actggtttac agcgcgtcgc
    80701 tttacgcgct gggaagcggt agtgcggcaa cgggcgggtt atatatgctt gcctttgcac
    80761 tgggtacgct gcccaatctt ttggcaatcg gcattttttc cctgcaactg aaaaaaatca
    80821 tgcaaaaccg atatatccgc ctgtgtacgg gattatccgt atcattatgg gcattatgga
    80881 aacttgccgt cctgtggctg taagccccat tcattcccga tacccgtaaa aaataccgtc
    80941 tgaaacctgt tcagacggca ctttcaccct aagcgttact cggcggcatt ttcctcacgg
    81001 gcaggcgcgt ccagcagggc tttgatggac agacggacac ggcctctgtc gtccacttcc
    81061 aatgctttca cgttcaccac ctgaccgact tgcaggtagt cgccgacatt gcgtacgcgc
    81121 tcgtgggcga tttggctgat gtgtaccaaa ccgtctttgc ccggcatcac gctgacaatc
    81181 gcgccgacgt tgttgtcgag gattttcacc acagtgcctt catacacttt gcccacttcc
    81241 acttcggcag taatctgctc gatgcgtttt ttcgccgcat cgccggcttc ttgagtggtc
    81301 gcggcaatgg taatcgtacc gtcttcggca atattgattt ccgtaccggt ttcagcggta
    81361 atcgaacgga tggtttcgcc acctttaccg ataacttcgc ggattttgtc ttggttgatt
    81421 ttcatcgtaa acaaacgcgg cgcgtgtgcg gacaactctt gcgggcccgc aacggcggct
    81481 ttcatctgat ccaagatgtg cagacgcgct tctttggcct gtgccaaagc gatttgcata
    81541 atttctttgg taatgccttg gattttgatg tccatttgca gcgcggtaac accttcggtc
    81601 gtaccggcca ctttaaagtc catatcgccc aagtggtctt cgtcgcccaa aatgtcggtc
    81661 aggacggcaa atttgttgcc ttccagaatc agacccatcg cgataccggc aacgtgtgct
    81721 ttcaaaggca cgccggcaga cagcaggctc aaacagccgc cgcagacgga agccatagag
    81781 gaagagccgt tagattcggt aatttcggag accacgcgca tggtgtagct gaaatcttca
    81841 ggtttcggca atacggccaa caatgcacgt ttagccaaac gaccgtgacc gatttcacgg
    81901 cgtttcggcg cgcccatgcg gcccacttcg ccggtagagt acggcggaaa gttgtagtgc
    81961 agcataaagc ggtcggtgta ttcgccggac agcgcgtcga tgatttgctc gtcgcgcgaa
    82021 gtacccaaag tcgctacggc aagggcttgg gtttcgccac gggtaaacaa tgcggaaccg
    82081 tgcgtgcgcg gcaatacgcc ggtttggatg ttcagcggac ggacggtgcg ggtgtcgcgg
    82141 ccgtcgatgc gcggttggcc gtccaaaatt tggctgcgga cgacatcggc ttccaagtgt
    82201 ttgaaaatgc ctttgatttc gttggctgcc agcgtgtcgg tttcttcggt aatcaaggct
    82261 tcttttaccg cactccaagc ttcgtccaat ttggcagaac gcgcttgttt ttgacggatt
    82321 ttgaacgctt ctttaatggt ttcgccggca atcccgcgga ctttggcaac cagttcctca
    82381 ttggtttcag gtgctttcca atcccaaact tccggattga cttcgtcggc aaattcattg
    82441 attgcattga tggcaacctg catttgatcg tggccgtaaa ccaccgcgcc cagcatcacg
    82501 tcttcgggca ggattttggc ttcggattcc accatcaaca cggctttgga agtaccggcg
    82561 accaccaagt ccaattgcga tttcgccaat tcggctttag tcgggttcaa aacgtacacg
    82621 ccgtttacat aaccgacgcg tgccgcgccg atcgggccgg caaacggtac gccgctcaac
    82681 accagcgcgg cagacgcgcc caacattgca ggaatatcag aatcgatttc aggatcgacg
    82741 gacacgacca tcgctacgat ttggatgtcg tggtagaaac cttcagggaa cagcggacga
    82801 atcggacggt cgatcagacg gctggtcagg atttcttttt cgctttgttt gccttcgcgt
    82861 ttgaagaaac cgccgggaat tttgcctgcg gcgtaagtgc gctcgaggta atcgacagtc
    82921 agagggaaga agtcttggcc ttctttcact tctttgttgg tggtaacggc aaccaaaacc
    82981 acggtgtcgc ccatagagac tttaacggca gcggcggctt ggcgggcaat ttcgccggtt
    83041 tccaaagtaa cggtgtggtt gccgtattgg aaagttttaa cgtgtttgtc gaacattatt
    83101 gttcctttca aaataccgca ctgctaaaac actaataatg cacactaaaa tccgaatgtg
    83161 catagttagg gtttcagacc gtgcggcagg ttgtaaacaa gctttcagac gacatttcgg
    83221 acgctgaaag caattccaga ttataaaggc aaccatcctt aaaatccagt attgatacaa
    83281 taaaaaggcc gtctgaacca tatttctttc agacggcctt caaacttaaa aatcaatcct
    83341 gagtcagcaa agtcaatgct tcccgatact tctcgacagt tttctgaatc acatcggcag
    83401 gcactttcgg cgcaggggct tttttgttcc aaccgctttg ttccagccag tcgcggacga
    83461 attgtttgtc aaaagacggc ggattggtgc cgactttgta ttggtcggta ggccaaaaac
    83521 ggctcgaatc gggagtcaat acctcatcca tcagcgtcag cgtaccgttt gtatccaaac
    83581 cgaattcaaa tttggtatcg caaataataa taccgcgcga tttggcatat tccgccgctt
    83641 cggtgtaaag ccgaaccgcc ttggcgcgca cttcttccgc caattctttg ccgataatgc
    83701 gtccgcattc ttcaaagctg atgttttcat cgtgatcgcc gactgcggct ttggttgagg
    83761 gcgtaaaaat cacttcaggc agttgttgcg cttcctgcat accttcaggc agttgaatac
    83821 cgcaaaccga gccggttttt tgataatctt tccaaccgct gcctgccaga taaccacgca
    83881 caatcgcctc tactttcacc ggagtgagct ttttagccac gacggcgcgt ttctctaaag
    83941 ctttggcttc gttttcaggc aaaacatcgt aaaccgtttg accggtaaag tggttgggca
    84001 taatatgcac cagtttttta aaccaaaaat tggaaatctg cgtcagaatc tcccctttgc
    84061 tcggaatcgg gtcgtccaaa atcacatcga atgcagacag gcggtcggaa gcgaccatca
    84121 gcatacgttt atcgtcgatt tcatataaat cgcgcacttt tccaaaatag atctttacca
    84181 aaccaatctc actcatttcg ccccccctga aaatatcttg aaaataccga cccgacaccc
    84241 gacaggtttg aatcacaaac caatattcta gccgaagtcg gcgcaaaaca atacccatgg
    84301 cacaaaaaac caacccgtca accgtcggca aagttttggc actataatac cgacagcaag
    84361 tcctacaata cacttttacc aaaggaaata cctcatgaga atcctattga caggctcgaa
    84421 aagccaactg gcacgctgcc tgcgcgaccg tcttccggaa gactgggaaa ccattgcgac
    84481 ggattccgca tccctagaca ttaccgatgc cgatgccgtc tgcaacatgg tcaaaagttt
    84541 ccaacccgac gccattgtca acacggctgc ctatactgcc gtcgacaaag cggaaggcaa
    84601 tgcggcagcg gcatttgccg tcaatgcttc cgccgtttac aaccttgcct tggcagcaca
    84661 tcgcgcccat gcccgattca tccacatctc aaccgactat atctttgacg gtaaagggaa
    84721 aaggccctat caggaaagcg actttaccaa tccttccaat gtatacggac aatccaaaac
    84781 cgcaggcgag ctgctcgcac tgtctgccaa tcccgacagc cttatcctgc ggacttcttg
    84841 gctgtttagc gaatacgggg acaactttat ccgcacgatg ctgaaccttg cgcgggaacg
    84901 ttccccgctg tccgccgtcc acaaccaaat cggctgcccg acctatgccg gcgacttgtc
    84961 cgccgccatc atccgcctgt tgcagcagtc caatcccgtt cgcggcattt accactacgt
    85021 cggcggcaaa tccgtatcct ggtacgaatt tgcccaacat attttccaag cggcatcgca
    85081 acagcagaca tccttccccg ttcccgaatt gactgccgtt tcagacaagg aatatccgac
    85141 cgccgccccc aggcccgcat acagcatttt ggactgccgc aaaatcgaaa acgacttcgg
    85201 catcaaaccg tcagactggc aaaaagccct tgcgcaggtc gtttccaagc tgctctgatg
    85261 ccgcccgcct ctccgtttcc gccgtcaagc accgccttgg cggttttctt atatagtgga
    85321 ttaacaaaaa ccagtactgc gttgcctcgc cttgccgtac tatttgtact gtctgcggct
    85381 tcgtcgcctt gtcctgattt ttgttaatcc actataaaat ctaccgatta cacaaacaca
    85441 tatcatcttt acacaatcat gcttccatca acagtaaaac atgatatgat tgccaacaat
    85501 aacatctcac aataaatttt ctaattttta ttgaaaaaat caataaataa gaattctccc
    85561 aaccgacaaa taaaataaag aaagggtcaa tatgcaacac cgaagaagac tggcaattta
    85621 ccaggcatcc aaacgtgctt cctttactgg caggtcatcc gccccgcaaa aacgtaaaga
    85681 acgttgattt gaaaaaaatg ccgcctgaag tcctgcttca gacggcattt ttttaccgtt
    85741 cgagaaactg tttcaacctg tcctcatcca aaaaccagtg gcagatttcc tcatctcccg
    85801 ccaacaaaac gggaaccagc tcattgtatt tttcttccaa aacaggattt tcatccacat
    85861 cgaccacttc cagcccgaac ccgtattcat tttgaaaagg tttgagtgca tcgcgcattt
    85921 tgtggcacaa gctgcaatat tcacgaaaca tcaaggtcaa tttcatccgc gtttccttat
    85981 ctgtcaattt gcacacgcca aagccttaga cgcagcagaa tcatggtcta tttgggaaaa
    86041 aacaatgttt tcaaggaaga tgatactcaa gtcctgccaa aaacagcaaa aatgccgtct
    86101 gaacagttca gacggcattt ccaaaaccgt tttacgcttg aacattgata cccgccgtaa
    86161 ccgtcggttg cgcattgtgc gccgtcagaa acgaagcggc aagctcccca cagcagccgc
    86221 agcagctcgc tacgcccaat tgcgactgca aatcgcccat tgtggtcgcg ccggcggcga
    86281 tggtttcctt gatttgatgg tcggtaacgg cattgcagat gcagacaaac attttgtgct
    86341 ccgtgtgttc tcaaactatc gtaccgatag cggcttttat tatcgtatgc gaatataaat
    86401 aaaaacggtt cgcattgcaa ggtcggtata cacggtttgt cttggtaatt ttttatcaag
    86461 tttgcatttt cgattgattt cttattaaac caaagtagaa gcctcagttt cgggaatggt
    86521 gttccttcac tacaccctgc aaccatacgt tggcaacatg ggtataaatc tgcgtcgtat
    86581 tcaaatcggc atgtcccaac atatcctgaa ccacgcgcaa atccaagccg tgccgcacca
    86641 gatgcgtggc aaaggcgtgg cgcaggctgt gcgggctgat gtgcccgatg cctgcctgac
    86701 ttgcatattc tttgacaatc atccatgcca actgacggga aatgcccgtc tttttctgac
    86761 tgacaaacaa tgcgtcgcaa ttcctgcctt tcagcagaag tgggcgtgcc tccgtataat
    86821 agcgttccac ccaatacgcc gactcctgcc ccatcgggac catcctctgc ttatcaccct
    86881 ttcccagcgc ggtaatacag cccctgtcca aatccacatt gccgaagttc agcccgaccg
    86941 cctcgctgac gcgcaagccg gtcgcgtaca tcaattcgag caaagccttg tcccgcaaac
    87001 cgtgcggcgt gtcggtatcc ggggcggcaa gcagtcggga aatctgctgc tcggtgatca
    87061 gggtcggaat attcttgtcg attttgggcg gtttcagcaa acgggtggga ttgtccgtcc
    87121 ttatgccttc acgctccatc catatataca ggcgtttgca tgccgataat gcgcgcgcct
    87181 gcgaactccg ttgctctccg tcaacataaa ccgccgccgc caaatccgct tcgtccgcat
    87241 ccttcagcat tctgcccgat tgggacaggc ggcgggcgat tttttccaaa tcgcgccggt
    87301 aaccgtttaa agtattctga ctgagccgcc tgtccaacca cagcgtttca agcagcctgt
    87361 cgatcaaacc ttcttccata ccgttccaaa caaatgccgt ctgaatcttc ttcagacggc
    87421 atggttcaca ttatcgggaa agcgtttcca atacttcctg cgcgtgaccc gccactttga
    87481 ctttccgcca ttcatgggcg atttctccat ccttattcaa gacgaacgta ctgcgctcga
    87541 tacctaacga ctctttcccg tacagtttct tcaatttgat gacatcaaac aggcggcaca
    87601 ctgtttcatc cttgtcgctc aacagctcga accggaaacc ctgcttggcg caaaaattct
    87661 gatgcgcctt tacgccgtcg cgggaaatac cgaccacggt ataacccaat gcctcaaact
    87721 gttccaaacg cgcattgaaa tccaagcctt ccgtcgtaca gcccagcgta ctgtctttcg
    87781 gataaaaata cacgaccaaa ggcagatgtt ctgccgaatg aaaatccgca ccgctgctcg
    87841 aaggcagggt aaattcatat ttcacatcca tagtcctact cccgatattc ccattattca
    87901 aaacggcacg cagacgcacc gccgcaattg ccaaaccaac cccgattcta ccgccccaaa
    87961 ggacaaggat tcaaccgccg gaaacatcca aaccgacaca cgacggcatg aaaaatatcc
    88021 atgtcaaacc acaaaatatg ttccgattta aaaacagaat gttataaaac ccaatcccca
    88081 aaacacaaca agaccgcccg cctacgggca gtctcctgtc agacgacata ctttacagat
    88141 ggctgttttt tcaacaaaat aacgccaata ctcaaaaata tggaatcaaa aatgtccatc
    88201 catactctga aacgcctgcc ctcatcgctg ctgctcggtc tctgcctttc cctgccgtca
    88261 gcccaccttt ttgccgacaa cgacatttta gggcaatttt tagaacagaa catgcttacc
    88321 tcctccgatc cgatagaaat attcgccgaa agcacgatac accccaccaa cacccaagcc
    88381 attacaggcg gtctgattct ctcctcacag tctgccctgg tcgtcaacaa caaaaccgga
    88441 cagatactgt atcagaaaaa cgccgacagg attatgccca tcgcctccat ttccaaactg
    88501 atgagcgcga tggtcgtttt ggatgcaaac ttggacatga acgaaaccgt taccattacg
    88561 cccgacgaaa tcgaccgcat caaagggacc ggcagccgtc ttgccatagg tacggcactt
    88621 acacgcaaag aactgctgca cctgagcctg atgagcagcg aaaaccgcgc cacccatgca
    88681 ttgggcagaa cctaccccgg cggcatgggc gcatttgtcg ccgccatgaa ccgcaaagcc
    88741 caaagcctcg gtatgtacgg cagccgcttt tacgaaccga ccggactcaa cttccaaaac
    88801 gtttccaccg ccaaagacct gagccttatg gtcaacgccg ccgcccaata tccgcaaatc
    88861 cgcaccaact cgacttccaa ctacgcctcg gtacagacca aaaacgggca gcagaactac
    88921 aaaaactcca atgcgctggt cagggaaggc atgtggaaca tcgaattgca gaaaaccggc
    88981 tacatacgcg aagcaggcag gtctatggtt gtcaaagcca acattcaaaa tcaacccgtt
    89041 accatcgtat tgctgaactc gcccacatcc gccacacgcg tcaacgacgc ccgcaaaatc
    89101 gaatcgtgga tgctgcagca acgctcctga catacaaatg cccggcggaa aaccgcccaa
    89161 acacaaaaaa aattggtaat ttttcctatt caaggctaca attcgacacg cacagggcat
    89221 ctgcccgatt tcacaatcat aatagcggag ttaaaaatat gacagctaaa ggacaaatgt
    89281 tgcaagatcc cttcctgaac gccctgcgta aagagcatgt tccggtttcg atttacttag
    89341 ttaacggtat caaattgcaa ggtcaggttg agtctttcga tcaatacgtt gttctcctga
    89401 gaaacacttc cgtcacccaa atggtttaca aacacgccat ttccaccatc gtaccggcac
    89461 gctccgtcaa cctacaacat gaaaacagac cccaagccgc accgacttcg accctcgtcc
    89521 aagtggaaac cgtccagcag cctgccgaat aatccgcacg aagcatgacg tgtcatatct
    89581 ttcaatacct taccggacaa cggtaaggta tttttatttt cagacagcat ttaaaaatgt
    89641 tattgcaaaa catccttcca ttcgcccatt gccttttgcg gaaggcactt cccgaaggcg
    89701 gcaatgcttt ggacggcacc gccggcaacg gacacgacac ccttttcctc gcacaaaccg
    89761 caggcatccg ggggaaagtg tgggcattcg acatccagcc gcaagccctg agccgcactc
    89821 gatgccatct gaaagaagcg agatacgaga atgtgcaact catcttagac ggacacgaaa
    89881 acctgaaaca atatgtttca gaaccactaa atgccgccat tttcaacttt ggctggctgc
    89941 ccggcggaga taaaagcctt accacccata cggaaaccag cattcccgcc ctttgcgcaa
    90001 ccctctccct gctgaaagaa ggcggtatgc ttattgccgt cctctatccg ggacacgaaa
    90061 acggcaaaca ggaggcagaa gcaatcgaac aatgggcaaa aaacctgcct caagaacagt
    90121 ttgccgtttt gagttacggc tttaccaacc ggaaaaacaa cccgccctat ctgttggcat
    90181 ttgaaaagtt acgtcaaaaa taaatgtttg cggtaaaata agccgattct aacccttatt
    90241 ttgatacgag ccgaccatgc agtatctgat tgtcaaatac agccatcaaa tcttcgttac
    90301 catcaccatt ttggtattca acatccgtgt tttcctactt tggaaaaatc caaaaaagcc
    90361 cttggtcggc ttttggaagg cactgcccca ccttaacgac accatgctgc tgtttacggg
    90421 attgtggctg atgaaaatta cccatttctc cccgttcaac gcgccttggc tcggtacaaa
    90481 aatcctgctt ctgctcgcct atatcgcatt gggtatgatg atgatgcgcg cccgtccgcg
    90541 ttcgaccaag ttctacaccg tttacctgct cgccatgtgt tgcgtcgcct gcatcgttta
    90601 ccttgccaaa accaaagtcc tgcctttctg aaacaccgtt atgaacaaca gacattttgc
    90661 cgtcatcgcc ctgggcagta atcttgaaaa ccctgcccaa caggtacgcg ccgcattgga
    90721 cacgctgtcg tcccatcccg atattcggct tcatcaaacc tcgtccctgt atgccaccgc
    90781 ccctgtcggc tatgacgacc agcccgactt tgtcaatgcc gtctgcaccg tttccaccga
    90841 tatggacggc atctcgctgc tctccttgct caaccgtatc gaggcggatt tcgggcgcaa
    90901 acgcagtttc cgaaacgcgc cgcgcacatt ggatttggac attatcgact ttgacggcat
    90961 ctccagcgac gacccccgcc tcaccctgcc gcatccgcgc gcacacgaac gcagtttcgt
    91021 catacgccct ttggcagaaa tcctccctga ttttgtttta ggcaaacacg gaaaagttgc
    91081 cgaattgtca aaacaactgg gcaatcaagg tatccgtctt ttaccggaca ggtaattccg
    91141 cacacagatg ccgtctgaat gccggtttca gacggcattt ttaccctgcc gccaatctgt
    91201 acgcaaaaaa aatcgcccct tgaaaaaggg gcgcaaaagg aacacaaacc actaccaaaa
    91261 ctttaaatct gcaacactgc ctgccgcata ctgtatccga caggatataa agccctcact
    91321 aaatcgtttc gagaaatcca aacttcttca tcgccgacag aaaatctgcc tttctccggc
    91381 accagctcca acaaaaacgg ttgaaccgcc gtatgcagcc tgtccttaca ccgccccagc
    91441 tttctggaca acgcggacaa ggcacgctta tagacactat ccttgcggtc aagttcccgc
    91501 gcactgccga cccagctcaa acgaaggttg cggctttcat cctcaatcag caaaatgcct
    91561 gttttgactt cccccacctc gggctcacat gaaagcgcaa tataatattt gccgtcgatg
    91621 ttgacatacg ccgcttcatg cacctgacag ttgcagccgg aagtggcaag cagcgaataa
    91681 acagagtccc gatgcagttt caaaatatca agggaaatct gtttgaaaat actttccggc
    91741 atttttccct cctttcgtct gctgcgttgt ttactggatt gactgcatta tacagacatc
    91801 tcccaatctt gcaaatgata attgttccta ttttttaaaa aatttataaa ttattgatgg
    91861 atatgtgaat taattttaag atttaaaatc tctttctcaa aaaaacacaa aaatgccgtc
    91921 tgaaacgtgt tcagacggca ttggctgcac agccagtccg tttaaaattt cacgccctta
    91981 tgcagcgcga cgatgcccgc actcatattg tgataatcca cgctgtcaaa gcccgcatcc
    92041 agcatcatct gtttcaaagt ttcctgatcg gggtgcatac ggatggattc ggcgagatat
    92101 tgataactct ccgcatcttt ggcaatcagt ttgcccatga ccggcagcag cttgaacgaa
    92161 tagaaatcat acgcgccttc caaaggtttg tagattttgg aaaactccaa taccagcaat
    92221 gtcccgccgg gtttcaaaac acggtacatc tctttcagcg cggcatcttt atgcgtcata
    92281 ttccgcaaac cgaacgccac ggaaaccaag ttgaaataat tgtcagggaa aggcagtttt
    92341 tccgcatcgg caagcgacac gggcaaaatc atgccttcgt tcaaaagacg gtcgcgcccg
    92401 acggtcagca tagaggaatt aatatcggtc agccaaacct cgccttcctt gccgacccgt
    92461 ttcgcccaac cgcgcgacaa atcgcccgta ccgccggcaa tgtccaacac tttatcgcct
    92521 tttttcaggt gcgccgtgtt gatggtgaaa tgcttccaca ccctgtgcag ccctgccgac
    92581 atcacatcgt tcataatgtc gtagtttttg gcgacggagt ggaacacttc cgccactttt
    92641 ccggctttct catcttcgtt gaccgtactg aatccgaaat gcgttttctg tccgcccata
    92701 ttctaaccac ctccctgatg ccgccctacg gcatcccaaa cttaacaatc tgattgcttt
    92761 tcaaaacggg aagcacccgc cgccgccaat ttgtccaaat attcctgcca catcgcatcg
    92821 tatccgtatg ccagtctgtg cagatacgcc caatcgtaaa gaccgctgtc gtgcccgtct
    92881 gaaaaactga ttttcagcgc gtactgtccg acaggctgca aatccgcgat ttggacatcc
    92941 gccttgccgg tctgcaaaac atcctgtccc acgccgtgtc cgcgcacttc cgcactcggc
    93001 gaatagacgc gtaaaaattc ggcaggcaga tttttaggtt cgtccccata agtcaaaacc
    93061 aacacggtac ggtttttcaa caaacggatt tcaacaggaa tattattcaa aaaacacctc
    93121 ggttcggcat acaatatccg gcaggcgcag gcgtgccttc aaacggcgcg tattttaaca
    93181 gctttatccc gataccgata ttcaaaccca cactttaaac ggcattgata tggacaatct
    93241 cgtatgggac ggcgttcccg acatccgcac actcgaccaa accatccgca aacacgcaca
    93301 cccgctcaac ctgattgtct gcctccccga taatcagatt cccgattttc aaaccgcaca
    93361 agatgcttcg gacgcggaat gccgtctgaa gcaccgtttg gatcaggcaa cgcagtgcct
    93421 ccagttcgac agcatcaacc tcatcgaaca catcctgccc gatgtccgct tctggctggt
    93481 tcccccttca cgcacccgcc gcctgcacga acacttccac cacatttcct ggcagaccga
    93541 agccatcccg caaaccgaaa gcaaacccga caaaccctgg tttgcacttc cacaaacatc
    93601 cgaacggcaa aaaccggaac acatcctcgt tatcggcgcg ggcatatccg gcgcggcaac
    93661 cgcccacgcc ttagcatcac acggcatttc cgttaccgta ttggaagccc gaaaagccgc
    93721 ccaagccgcc agcggcaacc gccaagggct gctctacgcc aaaatctcgc cgcacgacac
    93781 cgaacaaacc gaactgctgc ttgccggcta cggctacacc aaacgcctgc tcggacacat
    93841 cctgcccgaa tccgaaacct ggggcggcaa cggcatcatc cacctcaatt acagccgcac
    93901 cgaacaacaa cgcaatcacg aattgggttt gcaaaaacac cataaccacc tctaccgcag
    93961 catcacgcag gcagaagccg aaaaaatcgc cggtatcccg ctgaacacgc cctacgccga
    94021 accattatgc ggactgtttt ggcagcacgg cgtatggctc aatccacccg cattcgtccg
    94081 cgccctcctc cgccatccgc tgattggact acacgaagac acaccgttaa ccggtatttc
    94141 ccacgacgga gaaaagtgga ttgcaagcac gccaaacggc atcttttctg ccacacacat
    94201 cgtctactgc accggcgcgc acagcccctg cctgcccgaa atcaacctcg ccgccctgcc
    94261 cctcaggcaa atacgcggac aaaccggcct cacaccgtcc accccgtttt ccgaacaact
    94321 gcgttgcgcc gtttcaggcg aaagctacat cagcccgtcg tggcacggac tgcactgcta
    94381 cggcgcgagt tttattccca acagcagcca taccggatgg aacgaagccg aagaagcctc
    94441 aaaccgccaa gcattggcac accttaaccc cgcccttgcc gaatcattgt ttgccgccaa
    94501 cccaaacccc caaaaatacc aagggcacgc cgccatacgc tgcgacagcc ccgaccacct
    94561 tcccctagtc ggcgcactcg gcgacattgc cgccatgcgg cagacctaca ccaaactcgc
    94621 gctggacaaa aactaccgca tcgacacccc atgcccatac ctgcctaatg cctacgtcaa
    94681 caccgcccac ggcacacgcg ggcttgccac cgcccccatc tgcgccgccg ccgttgcagc
    94741 cgaaatccta ggcttgcccc atcccctctc aaaacgcctg cgccacgccc tacaccccaa
    94801 ccgcaccatc atccgcgcca tcgtcagaag gaaggatcta accccttaag cctttgccgc
    94861 atgccgtccc gaccacagaa aatcatattc aaataaacat accaatcctg acggtaaaat
    94921 atcgaaaaca cccataacgt tttcttccca aaataaggag taaggaatgc ctaacaaatt
    94981 gcctttgctg tccaagatga atgttccgta acattcgaag acttccttaa ccctgccagc
    95041 cgtttcggtt tctgtacccc ctgttagata aggaaggaat gaaaaagccc tgttgattgt
    95101 atgaagtaaa gggcgttaat ttttgttaat cttcccttct caggaactca atatataagg
    95161 atgttaattt ttatttccct tctcgaggac t
//
LOCUS       DAWFLT010000002        56098 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWFLT010000002 DAWFLT010000000
VERSION     DAWFLT010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 56098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56098
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.002"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            83..1267
                     /gene="coaBC"
                     /locus_tag="WGR74_000101"
     CDS             83..1267
                     /gene="coaBC"
                     /locus_tag="WGR74_000101"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ0904616.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTELPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKRLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMQIVERLAVYL
                     NK"
     gene            complement(1334..3490)
                     /locus_tag="WGR74_000102"
     CDS             complement(1334..3490)
                     /locus_tag="WGR74_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0904617.1"
                     /translation="MPVPQPTAPYDHLTAEARTLLFRTASYLKPEEQVELEKAVAYAF
                     HAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGVNIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDSCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLDQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3583..3789)
                     /locus_tag="WGR74_000103"
     CDS             complement(3583..3789)
                     /locus_tag="WGR74_000103"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ0904618.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(3848..4465)
                     /gene="gmk"
                     /locus_tag="WGR74_000104"
     CDS             complement(3848..4465)
                     /gene="gmk"
                     /locus_tag="WGR74_000104"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ0904619.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4593..5192
                     /locus_tag="WGR74_000105"
     CDS             4593..5192
                     /locus_tag="WGR74_000105"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ0904620.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKINIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5238..6026)
                     /locus_tag="WGR74_000106"
     CDS             complement(5238..6026)
                     /locus_tag="WGR74_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ0904621.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDTVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPP
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVRALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPSVDEDGVLRGLQDLGVI"
     gene            6351..7706
                     /gene="yegQ"
                     /locus_tag="WGR74_000107"
     CDS             6351..7706
                     /gene="yegQ"
                     /locus_tag="WGR74_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ0904622.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHDRNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLTGHSIAKQSQYVGHVTEIDENGWATIEVKNR
                     FAVGDSLEIIHPGGNQTIKLEQMARKGQPVDVAPGNGIQVKIPDMQGKEKALIARVLN
                     P"
     gene            7840..8316
                     /locus_tag="WGR74_000108"
     CDS             7840..8316
                     /locus_tag="WGR74_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HEZ0904623.1"
                     /translation="MLAACQSGSAPKNAHTASADTPAGESCKVIRDDTAGSGKNIIYR
                     CCNGQAAINAAIADGVLESGIPVSFGGGGVPIANGRNLVTRQAANSVGKSDSEACEHA
                     LINAARKFQQTAGKLGGRSVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
     gene            complement(8921..9307)
                     /locus_tag="WGR74_000109"
     CDS             complement(8921..9307)
                     /locus_tag="WGR74_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEZ0904624.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGISARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(9304..9552)
                     /locus_tag="WGR74_000110"
     CDS             complement(9304..9552)
                     /locus_tag="WGR74_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEZ0904625.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLTGIEKP
                     ARNAGRLKKFSHMENTDISRILEDDNETAALFFEESAL"
     gene            complement(9922..10245)
                     /locus_tag="WGR74_000111"
     CDS             complement(9922..10245)
                     /locus_tag="WGR74_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ0904626.1"
                     /translation="MITRPAQSDLVRIYNFLNDLGAERQANTVMRLLKNSFVSTQSSP
                     SNGKPYDLSVAGETLQNVREVIVPYGKSGYSYLFWHDKSGKQILILTVRHFRENAYHL
                     SFPFL"
     gene            10243..10350
                     /locus_tag="WGR74_000112"
                     /pseudo
     CDS             10243..10350
                     /locus_tag="WGR74_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(10356..10643)
                     /locus_tag="WGR74_000113"
     CDS             complement(10356..10643)
                     /locus_tag="WGR74_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904627.1"
                     /translation="MMLTNQEVTGVKLGYRKAVLQELARDFGGSVHSIVLDAVDGHIE
                     RLKAKQAYDKRAMQAFQDYRETGLHITLNEFADWVEGLGGDESKDMPECRK"
     gene            10707..10835
                     /locus_tag="WGR74_000114"
                     /pseudo
     CDS             10707..10835
                     /locus_tag="WGR74_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
     gene            complement(11025..12359)
                     /locus_tag="WGR74_000115"
                     /pseudo
     CDS             complement(11025..12359)
                     /locus_tag="WGR74_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448608.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
     gene            complement(12519..13148)
                     /locus_tag="WGR74_000116"
     CDS             complement(12519..13148)
                     /locus_tag="WGR74_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ0904628.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVEPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLKDLGEEPYKFDKELPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            13447..14781
                     /locus_tag="WGR74_000117"
     CDS             13447..14781
                     /locus_tag="WGR74_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ0904629.1"
                     /translation="MPPLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCR
                     ADVPQLDRGEAAFCPRCGYKLFRVGRHPFSAPPAYAATSLILMAFAYGMTYIEVGIPG
                     AASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALR
                     LATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSV
                     PQHWVYFQIGRLTGDNAVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLS
                     ISSAFLTAAVILYFPANILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSAS
                     ILVPVLKIAAMSVLIASARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFH
                     TYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKYD"
     gene            14774..16435
                     /gene="pqiB"
                     /locus_tag="WGR74_000118"
     CDS             14774..16435
                     /gene="pqiB"
                     /locus_tag="WGR74_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ0904630.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTLLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYAGDTVIATQGGGLDDLQVKLADLLDKFDKLPLDKTVAELN
                     GSLAELKSTLKSANAALSSIDKLVGKPQTQNIPNELNQTLKELRTTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            16435..16953
                     /locus_tag="WGR74_000119"
     CDS             16435..16953
                     /locus_tag="WGR74_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ0904631.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            16957..17508
                     /locus_tag="WGR74_000120"
     CDS             16957..17508
                     /locus_tag="WGR74_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ0904632.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFDEADIERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAV"
     gene            17498..18139
                     /locus_tag="WGR74_000121"
     CDS             17498..18139
                     /locus_tag="WGR74_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ0904633.1"
                     /translation="MPSEKPMNRRIFLLGAGALLLTACGRKSARTHAKIPEGSSVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVLV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(18132..18362)
                     /locus_tag="WGR74_000122"
     CDS             complement(18132..18362)
                     /locus_tag="WGR74_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0904634.1"
                     /translation="MISACPGFIHSRFGRSVMPSEPADHLFQKTPYRRNFMRLYGVGF
                     ALQTAYASRSSLVFQSVHDYINNLGADKPLFI"
     gene            18310..18669
                     /locus_tag="WGR74_000123"
     CDS             18310..18669
                     /locus_tag="WGR74_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904635.1"
                     /translation="MTDRPNLLWIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNIVFPLPAYPLAWLVPAGFMVLGGGFPLSLPLVALLVLTCCILAHCPPLS
                     RLLCCPCPNHPMSKNSA"
     gene            18700..21552
                     /gene="gcvP"
                     /locus_tag="WGR74_000124"
     CDS             18700..21552
                     /gene="gcvP"
                     /locus_tag="WGR74_000124"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ0904636.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDEFVGNTVPQ
                     SIRMPSELDLPDALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVSDFAKADDGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDAFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGINVVHKVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSACEDLVDLYRAFTGKDTFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTAGYRELLADMENSLKSITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAQRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIVHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHALTDTHSASADQT
                     AVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVGRGEWPKEDNPLVNAPHTASDVTGEWAHPYSREEAVFPL
                     PFVREHKFWPSVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(22017..22856)
                     /locus_tag="WGR74_000125"
     CDS             complement(22017..22856)
                     /locus_tag="WGR74_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ0904637.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            23141..23216
                     /locus_tag="WGR74_000126"
     tRNA            23141..23216
                     /locus_tag="WGR74_000126"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23174..23176,aa:Arg,seq:ccg)
     gene            complement(23281..24474)
                     /locus_tag="WGR74_000127"
     CDS             complement(23281..24474)
                     /locus_tag="WGR74_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0904638.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIATVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(24495..25583)
                     /gene="trmA"
                     /locus_tag="WGR74_000128"
     CDS             complement(24495..25583)
                     /gene="trmA"
                     /locus_tag="WGR74_000128"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ0904639.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDDEWMQAAQALQQQLDTSVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGGFTQPNAAVCQKMLEWACGAAEGLGGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVGAAQWNIEANGIGNIKIARLSAEEFTEAYTGKREFARLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLVETH
                     VVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            25730..26830
                     /gene="aroC"
                     /locus_tag="WGR74_000129"
     CDS             25730..26830
                     /gene="aroC"
                     /locus_tag="WGR74_000129"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ0904640.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDIHVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVQVNTPDITLSNK"
     gene            26922..27326
                     /locus_tag="WGR74_000130"
     CDS             26922..27326
                     /locus_tag="WGR74_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ0904641.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAEAAGSSAAE"
     gene            complement(27400..29385)
                     /gene="parE"
                     /locus_tag="WGR74_000131"
     CDS             complement(27400..29385)
                     /gene="parE"
                     /locus_tag="WGR74_000131"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ0904642.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVHDNGRGIPVGLHPEEGVSVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGIEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPIFSCENYISDDHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKNKPARKFYA
                     LDQNELDSILERLQKEGFKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(29452..29976)
                     /locus_tag="WGR74_000132"
     CDS             complement(29452..29976)
                     /locus_tag="WGR74_000132"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ0904643.1"
                     /translation="MLDREGYRPNVGIILTNNRDEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIEFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQPSGNR"
     gene            30141..31436
                     /gene="serS"
                     /locus_tag="WGR74_000133"
     CDS             30141..31436
                     /gene="serS"
                     /locus_tag="WGR74_000133"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ0904644.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLKHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDESKTTQYLIPTAEVTLTNTVAGSILDEGGLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGATKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(31514..32512)
                     /locus_tag="WGR74_000134"
     CDS             complement(31514..32512)
                     /locus_tag="WGR74_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0904645.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEAEKIGGRYVELNELYARSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(32637..33713)
                     /gene="prfA"
                     /locus_tag="WGR74_000135"
     CDS             complement(32637..33713)
                     /gene="prfA"
                     /locus_tag="WGR74_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ0904646.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYQLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLP
                     KDADDDKNIFIEVRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(33803..34573)
                     /locus_tag="WGR74_000136"
     CDS             complement(33803..34573)
                     /locus_tag="WGR74_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0904647.1"
                     /translation="MNFTDTHCHLADAALRENLPHILTAARKVGVKRFIVPATCPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDCVRLEAMLARYPQAWVGEIGLDFY
                     DKTQTPPQRERQIQVFVRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANILWIAEIAAQIRGVGVADIAAITERNADSLLRP"
     gene            complement(34688..35677)
                     /locus_tag="WGR74_000137"
     CDS             complement(34688..35677)
                     /locus_tag="WGR74_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ0904648.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGPLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGALAEWDETQGWHNVRIPNQDAADVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTPADEGFIRAVQDFTQQGKLLLNISQVRQGK
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGKEEVEKELVRLKL"
     gene            complement(35744..36427)
                     /locus_tag="WGR74_000138"
     CDS             complement(35744..36427)
                     /locus_tag="WGR74_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0904649.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(36616..37950)
                     /gene="glmM"
                     /locus_tag="WGR74_000139"
     CDS             complement(36616..37950)
                     /gene="glmM"
                     /locus_tag="WGR74_000139"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ0904650.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            complement(38080..38937)
                     /gene="folP"
                     /locus_tag="WGR74_000140"
     CDS             complement(38080..38937)
                     /gene="folP"
                     /locus_tag="WGR74_000140"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ0904651.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRSGADYVSPEEEWARVEPVLAEVAGWGVPISLDTRR
                     TVIMEKALALGGVDIINDVAALNDEGAVELLARQADTGICLMHMQGLPKTMQINPKYQ
                     DVVGEVARYLKARSAECIAAGIAPQRIILDPGFGSGFGKPLQHNIALMRHLPELMAET
                     GFPLLIGVSRKSTIGELTGEANAAERVHGSVAAALASVARGAQIVRVHDVKATADALK
                     VWEALGINL"
     gene            complement(39077..39970)
                     /locus_tag="WGR74_000141"
     CDS             complement(39077..39970)
                     /locus_tag="WGR74_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ0904652.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVCIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            40061..42175
                     /locus_tag="WGR74_000142"
     CDS             40061..42175
                     /locus_tag="WGR74_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ0904653.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTAGGEYAQWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKGHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(42240..43718)
                     /gene="ubiD"
                     /locus_tag="WGR74_000143"
     CDS             complement(42240..43718)
                     /gene="ubiD"
                     /locus_tag="WGR74_000143"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ0904654.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPIKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVIGMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(43738..44253)
                     /locus_tag="WGR74_000144"
     CDS             complement(43738..44253)
                     /locus_tag="WGR74_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ0904655.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPYPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLLIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENVVYF
                     GMICNWLPPYETDTFGLHCNVVTQSDNQRPLLQLHQSGGHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(44260..44790)
                     /locus_tag="WGR74_000145"
     CDS             complement(44260..44790)
                     /locus_tag="WGR74_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904656.1"
                     /translation="MVRQGEATLYWFLLIHYHYGREAGWFVWLAAAMCGLWLLRALTA
                     KLPQQRYAALFITLFFAAVLVFGRRDSMYWYPVLVNLMMLAVFGGSLFAGQTVIEKLA
                     RLQQPDLPEKAVRYTRRVTQVWCVFFIINGTLAALLARLGRYDWWAVYTGVIAYVLMG
                     MPFAGEWLYRKLVLKV"
     gene            complement(44993..45562)
                     /locus_tag="WGR74_000146"
                     /pseudo
     CDS             complement(44993..45562)
                     /locus_tag="WGR74_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691695.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            45602..46212
                     /locus_tag="WGR74_000147"
                     /pseudo
     CDS             45602..46212
                     /locus_tag="WGR74_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            46157..47293
                     /locus_tag="WGR74_000148"
     CDS             46157..47293
                     /locus_tag="WGR74_000148"
                     /inference="COORDINATES: protein motif:HMM:NF006587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEZ0904657.1"
                     /translation="MDWHERFPELEAQLGAPIEDYAPPKHWTRKQLRSMGRVSYLCVD
                     AAEQALTDAGLLGDESITDGRMGVACGSSSGSTKDIGDVGELLLTGMSRNFSANTYVR
                     MMPHTAAANIGIFFGLKGRIIPTSSACSSGSQGIGYAYEAIKYGLTDMMLAGGGEEFF
                     PSEVYVFDSLYAASRRNGEPEKTPRPYDANRDGLVIGEGAGIFVLEELEHAKRRGAII
                     YAELVGYGANSDAYHISTPRPDAQGAILAFQTALQHAGLAPEDIGWINLHGTGTHHND
                     SMESRAVAAVFGNNTPCTSTKPQTGHTLGAAGAIEAAFAWGIADRQSNPEGKLPPQLW
                     DGQNDPDLPAINLTGSGSRWETEKRIAASSSFAFGGSNCVLIIG"
     gene            47347..47422
                     /locus_tag="WGR74_000149"
     tRNA            47347..47422
                     /locus_tag="WGR74_000149"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47380..47382,aa:Ile,seq:cat)
     gene            47497..48255
                     /locus_tag="WGR74_000150"
     CDS             47497..48255
                     /locus_tag="WGR74_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ0904658.1"
                     /translation="MKKVSVLIVAKNEANHIQECIESCRFDKEVIVIDDYSTDNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            48256..49320
                     /locus_tag="WGR74_000151"
     CDS             48256..49320
                     /locus_tag="WGR74_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ0904659.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKAKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGD
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAGH
                     QCVANRFTINHTADKLLDAI"
     gene            49554..50891
                     /locus_tag="WGR74_000152"
     CDS             49554..50891
                     /locus_tag="WGR74_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0904660.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTILVALPVQLAEFYIGRTGGKNAVDSFRVLRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIAATIRQDER
                     KRKKHTWLIGTAIFIIGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLDIL"
     gene            complement(51126..51461)
                     /locus_tag="WGR74_000153"
                     /pseudo
     CDS             complement(51126..51461)
                     /locus_tag="WGR74_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243776.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
     gene            complement(51442..51723)
                     /locus_tag="WGR74_000154"
     CDS             complement(51442..51723)
                     /locus_tag="WGR74_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEZ0904661.1"
                     /translation="MGRHFGRRRFLTAAAVAAAGAAVSFLPNPFAADDEKRNGDEKRS
                     ENVFFWKGVALGSGAELRLFGVDDRRAADLVNKVLAEVARLEKIVQPLP"
     gene            complement(51732..52526)
                     /locus_tag="WGR74_000155"
     CDS             complement(51732..52526)
                     /locus_tag="WGR74_000155"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ0904662.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVLEQIKKNPDSRRLIVSAWNPALVDEMALPPCHTLFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            52910..54244
                     /gene="gdhA"
                     /locus_tag="WGR74_000156"
     CDS             52910..54244
                     /gene="gdhA"
                     /locus_tag="WGR74_000156"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ0904663.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFEKQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            54386..54760
                     /locus_tag="WGR74_000157"
     CDS             54386..54760
                     /locus_tag="WGR74_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904664.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            54804..55061
                     /locus_tag="WGR74_000158"
     CDS             54804..55061
                     /locus_tag="WGR74_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HEZ0904665.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQQ"
     gene            complement(55247..56026)
                     /locus_tag="WGR74_000159"
     CDS             complement(55247..56026)
                     /locus_tag="WGR74_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ0904666.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT        13465 a        15463 c        13916 g        13254 t
ORIGIN      
        1 ttgtatccaa acggtatcta atgtatccac actttgttat agaatggctg ctgttttttc
       61 ttcgtaatta gaaattgtca aaatgggcaa acatattctt ttaggtgtaa cgggcagtat
      121 tgcggcgtat aagtcttgcg agttggttcg tctgctgaaa aaacaggggc attcggttac
      181 ggtggttatg agccgctcgg caactgaatt tgtttctccg ctgacttttc aggctttaag
      241 cggcaatcct gtcctgaccg acacgcacgg cggcaacggt tcaaacggta tggaacatat
      301 caacctgacc cggaatgcgg atgtttttct gattgcgccg gcaagtatga ataccgtggc
      361 aaaaatctgt aacggcgtgg cagataacct actgaccagt ctggcagccg cacggaaatg
      421 tcctcttgcc atcgctcccg cgatgaatgt ggaaatgtgg ctcaaccctg ccaaccaacg
      481 gaatatcgca caactggttt cagacggcat tactgtctat atgccgggct tgggcgaaca
      541 ggcttgcgga gaaaatggta tgggaaggat gccggaacct gccgaattgc tggatttgct
      601 tccagacttg tggacaccga aaattttaag gggcaagaaa atcttgatta ccgcaggtgc
      661 gacatttgaa gccattgacc ctgtcagggg catcacaaat acctccagcg gaaaaatggg
      721 ggtagctttg gcgcgggcgt gccgtgccgc cggtgcggaa gtcagcctga ttcacggaca
      781 gcttcaaacc gaactgcctt tcggcatatc cgatacggtt caagccgtca gcgctgaaga
      841 tatgcatcgc gcagtgcatc gtttgattga aaaacaagat gcttttattt ctgttgccgc
      901 cgtctcagac tataaggtta aaaacaggag tacgcaaaaa ttcaaaaaag ataaaaatgc
      961 caaacggtta tccatcgaat tggatgagaa ccccgatatt ttggcttcta ttgcctcatt
     1021 gccgaacccg ccgttctgca tcggttttgc cgctgaaacg gagaatgtaa tggcatatgc
     1081 acgagaaaaa cgtattaaga aaaaaatacc ggtaatagtt gccaatgatg tttcaatcgc
     1141 gatgggcaaa ccgaccaacc ggattaccat tatcggggac gacggggaac tatcttttcc
     1201 cgaaacaagt aaagatgaag cggcaatgca gattgttgaa aggcttgccg tatatttgaa
     1261 caaataagca attgaacgga taaaccataa agcgggttgc ctgttaatca aaaggcaacc
     1321 cgttttacct gcttcaactt ctgatgactt tgcggatata tggaatacta tgcagatttt
     1381 gaataatctg attcaactga tccaaattct tgactttcaa taagaatttg aattcgacaa
     1441 aaccttccgt tcccgactgg gatttggatg gtgtttcgac cgactcgatg tccgcaccgg
     1501 aatcggaaat cgcttgcgcc attaatgcca acaggccgtg gctgtcttcc gattggactt
     1561 gaagcccgac acggtagttc tgcccattca tattttccca gtctgcatcc agctgctgtt
     1621 cgggatcgga cttcaacaac gtcgggcagc tatccctatg gataatcatg ccttttccct
     1681 taaccaacag caaacggatg gaatcgccgg gaacagggtg gcagcactct gcaaaatgaa
     1741 tatgcccgct ttcctgacca tcgactttaa tggaactgag cctgacctcg ctgccgaaat
     1801 gctcccctgc caactcagca atgtgcatgg cgacataaac aggcagggta tgccccatac
     1861 cgacgttgta cagcacttcc tcaaatgaag tctgtttgtc gttgagatcg gcaaggtatt
     1921 tttctttgat gccgtctgaa agcaggacat ctttgggcag caaactggac agggcttttt
     1981 gtaagaggct ctctcccaaa acgaccgcat cgtgccggtt aaggttttta atatattggc
     2041 gtatggcact gcgcgccctg cctgacacgg cgaaattcaa ccatgcagga ttgggtttgg
     2101 catgttcgga tgtaataatt tcgacggaat cgccggtttt gagcttcgta cgcaacggca
     2161 tcatgatatt gttgatacgt gcggcaacgg ttttgtgccc gatatcggta tgcaccgcat
     2221 aagcaaaatc gacaggcgtt gcccctttgg gcaaagttag gatttttcct tttggcgtaa
     2281 ggatgtagat ttcgttcgga aacaaatcga ctttgacgtg ttcgagaaac tcaatggcat
     2341 tggcactgct tgcctgcaaa tctaagatat ttttcagcca ccggtttgtg tgaagcaccg
     2401 cctgatcgac cgtcttagaa tatgatttat agctccaatg tccggcgatt ccaccttcgg
     2461 caacagcatc catttccttg gtacgtatct gaacttcaat cggcaagccg taaggaccga
     2521 ccaaagtcgt atgcagactt tgatacccgt tgcttttcgg aatggcgata tagtctttga
     2581 accgcccggg cttgggctga tagagggtgt gcaatgcgcc gagtgcggca taacaggctg
     2641 gaatgctgtt gacaatgacg cggaaaccgt aaatatccat aacctcggca aagcgcagct
     2701 ttttcgccat cattttctga tggatgccgt acaggttttt ttccctgcct ttgattttgg
     2761 cctctatatt cacacccacc agccgctggc cgaatgcgcg caacactttg ccgacaacgt
     2821 cctgccggtt cttccggctc ttgtccatcg ctttttttaa agtctcgtag cggttgggat
     2881 gcaggttttg gaacgataaa tcctgaagct cttgatatgc gttattcaaa cctatacggt
     2941 tggcaatctg tgcatagatt tcaagggttt cccttgcaat ccggcggcgt ttgtccgggc
     3001 gcatcgaacc gagtgtccgc atattatgca gacggtcggc aagtttaacg acaatcacgc
     3061 gcacatcttt ggtcattgcc aaaatcagtt tgcggaaact ctccgcctga tgttccgcat
     3121 gatcttcaaa tttgagtttt tcgagtttgg acagaccgtc caccatctcg gcaatcgtat
     3181 tgccgaacac cgccgccatt tccccttttg tcacgcccgt atcttccaat acgtcgtgca
     3241 tcacgcctgc acacagaccc tgtatgtcca tatgccaaag ggcgagctgc gtcgcaacgg
     3301 caattggatg cgtaatgtat ggttcgccac ttttccgggt ttgtccgtca tgagcgtgaa
     3361 atgcataggc aacggccttc tcgagctcaa cctgttcttc aggcttgaga taggaagcgg
     3421 tacggaaaag caaggtgcgt gcttcggcag ttaaatggtc gtaaggtgcg gtgggttgag
     3481 ggacaggcat ttcagacggc tttcggaaca tattttattt ttcgtttcaa accgtcggac
     3541 tgcacggcgg cagaatgtgc cgacgtgcgt ttccggcaga atttatttat tgcgcgtcaa
     3601 cagttccgta ccgatatgcc cggcggcgat ttcccttaag gcggtaacgg tcggtttgtt
     3661 attgcggaca tcgtccacaa gcggcgtatt gccattttca agctggcggg cccgacgggc
     3721 cgctaccaat gtcaggtcaa aatggttgga aatttttccg gtacagtctt cggtggtaat
     3781 acgtgccata ttatttgctt tctttcaaaa atatttaaaa taggaaactg ggtattttcg
     3841 cggtttttta ggaattttcc aacaaatctg caataaatcc cagttgccgc gaccttttca
     3901 gacggcaggc attcacaata tggcgcaaat cctcctccgc ccgcgccaag tcatcattga
     3961 ccacgacaaa atcgaacaat acggactgtt cgatttcatg ccttgccttc gacagcctcc
     4021 tttggataac ttcccgactg tccgtcccgc gtccgttgag acgcgcagca agcacatcga
     4081 aagacggcgg caggataaag atgccgacag cttcaggcag cgcatctcga acctgcgccg
     4141 cgccctgaac gtcgatttcc aaaatcacgt cataacctgc cgccgccaac gcattcacgc
     4201 cctccgcgct tgtgccgtaa tagttgccga atacgtctgc gtattccaaa aaagcctcct
     4261 gtgcgataag cgactcaaac tcttctttgg aaacaaagtg ataatgtacg ccgtttgctt
     4321 cgccttcacg cggcgggcgc gtcgtgtgcg acacggaaac gcgcaaaccg ttatggtttg
     4381 ccaacagccg cgacaccagc gtggttttgc ccgtgccgga agcggccgaa atgataaaga
     4441 tgttgccttt tcgataagcg gacatatttt ttacctgtat attttccagc cgattgtatc
     4501 acaatggaca cccagtttcc tatttgccga tgcccatatt ttgccgctat tgttttgatt
     4561 gatttggcaa gcggcaggct gacggctaca atatggcgtt aaaaacatca aacttggaac
     4621 acgcaatgct ggttcatccc gaagctatga gtgtcggcgc gcttgccgac aaaatccgca
     4681 aaatcgaaaa ctggccgcaa aaaggcatct tattccacga catcacgccc gtcctgcaaa
     4741 gtgcggaata cttccgcctt ttggtcgatt tattggttta ccgctatatg gatcagaaaa
     4801 tcaacatcgt tgccggtttg gacgcacgcg gcttcattat cggcgcggca ctcgcctacc
     4861 agctcaatgt cggtttcgtc cccatccgca aaaaaggcaa gctgcctttt gaaaccgtat
     4921 cgcaaagcta cgcgctcgaa tacggggaag ctgcggtgga aatccacacc gatgccgtca
     4981 aacccggttc gcgcgtgctg ctggtcgatg atttggttgc cacgggcggc acgatgcttg
     5041 ccgggctgga actgatccgc aaactcggcg gagaaattgt cgaagccgcc gccattttgg
     5101 aatttaccga ccttcaaggc ggcaagaata tccgtgcaag cggcgcgccc ttatttaccc
     5161 tgcttcaaaa cgaaggctgt atgaagggat gaaaaccgac cctgccgtct gaaaccggca
     5221 gggttgttat tatgcgttca aatcacgccc aaatcttgca agcccctcaa cacgccgtct
     5281 tcatcaacgc tggggcaaac atatttcgcc gcttctttcg ccgcctgttc cccgttgccc
     5341 attgccacgc cgaacccgac ttcagacagc atttccacat cgttcaaacc gtcgccgaac
     5401 gccatcacgt ccgccatttc caaacccaat gcacgcacca cgcttctgat gccgtctgtt
     5461 ttcgacattc ccgcaggcag cagatcgacc gcttcctcgt gccagcgcac cgttttcaag
     5521 ccttcccgtt ccacaatatc cgaccagggc ggcatttcgt tttcctccgc aaacaccagc
     5581 atctgataca ccggtttgct tgaaaaataa tccttatcgg caaaaaaatc gctggcgata
     5641 tgctgcaagg cgcggcacac gcattccgac agcgcggaca cggcgatccc ctctccgccg
     5701 acaaacgcat aatccatacc caagccatcc aaatgcgcgc aaaccctgcc catcaaaccg
     5761 gcatccatcg gtacttcgcg cacagttttt ccgtgcagca gcgcaaactg cccgtttatc
     5821 gttaccacgg tatccatacc cgtttccgcc atcatgtccc tgaccttttc gggaatcgtc
     5881 gccaaagacc gccccgttgc caacgccgtc aatatgcctt tgccgcgcaa agccgccacc
     5941 gccgttttca cggaaggacg caaggtatcc gtatatttgc ggtacagcgt atcgtcaata
     6001 tcgaaaaaca cgattttggg attcatcata ttctctctcg cactcaaact gccgcattgt
     6061 atcccaatca ggcaaatact tgataaatcc ttataaattt tccggcaaaa ttgaccgcga
     6121 acacgaaaag gcggataatc cgcccatcct caaacccttt tcagacggca tttgcagcaa
     6181 tgccgtctga aacattttta caaagcacac aaatcatgtt tcaacacaca ggacgacaca
     6241 taaagcgtcg ccctatatgt tgccctgatt cggaaagggt tacacccctc ccaaataaag
     6301 tctgattcta ccgccctaaa gggcggggtt tcaaccgaaa aggaaacacg atgaaagcac
     6361 ccgaactttt attaccggca ggcggtctcg aacgtatgcg cgccgcctac gattacggcg
     6421 cagatgccgt ttacgccggc agcccgcgtt actccctgcg cgcccgcaat aacgaatttg
     6481 ccaaactcga cgttctcgag caaggcatta aagaagcgca tgatcgcaac aagaaattct
     6541 tccttaccgt caatacgctg ccgcacaact ccaaactcaa aaccttcgtt tccgatatgg
     6601 agcccttgat tgccatgaaa cccgacgcgc tgattatggc ggatccgggc ttgattatga
     6661 ccgtgcgcga aaaatggccg gaaatgccga tccacctgtc cgtacaagcc aacaccacca
     6721 actactgggg cgtgaaattc tggcagaata tcggcgtcga acgcatcatc ctgtcgcgcg
     6781 aattgagtat ggaagaaatc gccgaaatcc gccaacaatg ccccgacatc gaactcgaag
     6841 tcttcatcca cggcgcattg tgcatcgcct actcaggccg ctgcctgttg tcgggctatt
     6901 tcaaccaccg cgaccccaat caaggcacct gcaccaactc ctgccgttgg gactacaaag
     6961 tccacaacgc tacggaaagt gatgcaggcg atgcccagct tctgcaaggt tttaactttg
     7021 aaaaagccca agaagaagcc aaccaaaact ttgaaggcat caacggtcaa aaacgccatc
     7081 cctacgccga caaagtcttc ctgattgaag agtccaaccg cccgggcgaa atgatgccga
     7141 ttatggaaga cgaacacggc acctacatca tgaattccaa agaccttcgc ggcattgaag
     7201 tggttgaaaa actcgccaaa atcggcgtgg acagtctcaa agtcgaaggc cgcaccaagt
     7261 cgctttatta tgttgcacgt gtcgcccagt cctaccgcaa agcgattgat gatgccgttg
     7321 caggccgtcc gtttgattac agcctgttga gcgaactcga aggcctcgcc aaccgcggct
     7381 acaccagcgg cttcctcgaa cgccaccaaa cccaagacta tcaaaactac ctgaccggcc
     7441 actctattgc caaacaaagc caatacgtcg gacacgttac cgaaatcgac gaaaacggct
     7501 gggcgaccat cgaagtcaaa aaccgcttcg ccgtcggcga ttcactcgaa atcatccacc
     7561 cgggcggcaa ccaaaccatc aaattggaac aaatggcccg caaaggccag cctgtcgatg
     7621 tcgcacccgg caacggcatt caggtcaaaa tccccgatat gcagggcaaa gaaaaagccc
     7681 tcatcgcacg cgtattgaac ccctgagcca caatgccgtc cgaaccccgt tttcagacgg
     7741 cattttgttg aatcttacgg tacaatggca atgatttccc acagcaaaca aggacatccg
     7801 acatgaaaaa aaatcctcat actctccgcc gccgccctga tgctggcagc ctgccaatcc
     7861 ggcagcgcac ctaaaaacgc gcacacggca tccgccgaca caccggccgg agaatcatgc
     7921 aaagtcatcc gggacgacac cgcaggaagc ggcaaaaaca tcatttaccg ttgctgcaac
     7981 ggtcaggcgg caataaatgc agccattgca gacggcgtat tggaatcagg gattcccgtc
     8041 agcttcggcg gcgggggcgt accgattgca aacggcagga atctggttac acggcaagca
     8101 gcaaacagcg ttggcaaatc cgactccgag gcctgcgaac acgccctaat caatgccgcc
     8161 cgcaaattcc agcagaccgc cggcaaactc ggcgggcgca gtgttaccgg cttccacagc
     8221 tacttcgaca agcaaccgct gcaaggagga caatacgact gtcaggcagg ctcgttccac
     8281 gtccgcgtgg tcatgcgcgc caatgtcgtc cgttaacata tcggcgacca aaaaattagg
     8341 ctgtgtttta gtaatctgtt gatttcaatt atttgcaaag gaaaagacaa ttattttttc
     8401 ggttaggaag agacctatcc tattgaatac atttaagtta aatacgtcta tcaacactat
     8461 attatagtgg attaacaaaa atcaggacaa ggcgacgaag tcgcagacag tacagatagt
     8521 acggaaccga ttcacttggt gcttcagcac cttagagaat cgttctcttt gagctaaggc
     8581 gaggcaacgc cgtactggtt tttgttaatc cactataaaa cacagccata tatattaagt
     8641 acctgctctt tatgcgcttt tttaggaaag gaagatcaaa cggttcaata gcgaagtctt
     8701 gcccaaattt gatggacagg atatagaata catgcgtgca taccacaccc tgccaaagtt
     8761 gggagatgca cttaaggttg cacttgtggt cgccaactat gcctatacac gcggatttcc
     8821 cgcgtttata ggccgcgacg gaagtctggc ttttaagcga agacaataac cgaaaagacc
     8881 gcctcggcgg tctttcttgt gtacacagtt tttatatttg tcagcttgat gcattgacaa
     8941 ctctaattcc atattgcgga atatattcat cgacagtcat cagttcaaag ccttccgctt
     9001 gggtttgtgc aatcaacatc ctatcgaaag ggtctttgtg tatttccgga aggcttccag
     9061 cctgttttgc atgaaacaga cctataggca gcatttcaaa atcctcttct tgaagcacat
     9121 caaaaaactc ttccggtaat ttcaacaacc ccttgttctg cttgatggaa atttcccaaa
     9181 tacttgctgc actgacaaag atcgcatttc tcggattttc tatcagtttg cgtgcagata
     9241 tccccagttt cttgtcatcc aacaaccacc acagcaaagc atgggtatca agcagaatct
     9301 ttctcacaga gccgactcct caaaaaataa agctgccgtt tcattgtcat cctcaagaat
     9361 acgtgaaata tccgtatttt ccatatgact gaattttttc aaccttcctg catttcgtgc
     9421 cggtttttca ataccggtta gttggacgca aggcttacct gccttcgcaa taataacgat
     9481 ttcccctgct tctgctcttt gaatcaattg actcaaattg gtttttgcct gatgaatatt
     9541 tgcttgaaac ataacacttc tcatgattag ctaacttgac taatatacat cattaccaag
     9601 attttgggaa tctcattaca tatatttgat tatatccgcc gttttattca caccttgcta
     9661 tttagtaaca atcttcggca accaaaaatg ccgtctgaaa catttttcag acggcatttt
     9721 taatcccctt gcctgattgt gcggcagatt caaatcgggg cactccatac ttccatatct
     9781 gcaccgttca aacatcccgc ctgcggaacg gaatcaaagc ggcagtccgg ttatgttctt
     9841 gaaggcggtt tatccgaacg tgccgcaata aagccgaaac gcattgcccc gcacaaggga
     9901 aagccgccaa accaaatgac gttataaaaa aggaaagctc aggtggtagg cattttcacg
     9961 gaaatgcctc accgtcaaaa tcaatatttg cttacctgat ttatcgtgcc aaaacaaata
    10021 gctataaccg cttttgccat agggcacgat tacttccctt acattttgca aagtttcccc
    10081 cgccaccgac aaatcataag gtttcccatt actcgggctg ctttgtgtag aaacaaaaga
    10141 atttttaagc agccgcatca ctgtatttgc ctgacgttcc gcacccaaat cattcaaaaa
    10201 attataaatc ctgaccaaat cagattgagc ggggcgtgta attatagtgg attaaattta
    10261 aatcaggaca aggcgacgaa gccgcagaca gtacagatag tacggcaagg cgaggcaacg
    10321 ctgtactggt ttaaatttaa tccactatat caacttcact tgcggcattc gggcatatcc
    10381 ttcgattcat cgccacccaa gccctccacc cagtccgcaa actcattaag cgtaatatgc
    10441 aatcctgttt cccgataatc ctgaaaagcc tgcatcgccc gcttatcgta ggcttgtttg
    10501 gctttcaagc gttctatatg accatccacc gcatccaata cgatactgtg cacactgccc
    10561 ccgaaatccc gggccagctc ttgtaaaacg gctttgcggt atcccagttt gacaccggtt
    10621 acttcctgat tggtaagcat cattattttc ccataaatca aacgtctgac acggcattat
    10681 aaacacaatg cggcatctgc cgccaccttt gcggacgcag tgttaccggc ttccagagct
    10741 acttcgacaa gcagccgctg caaggcggac aatacgattg tcaggcaggc tcgttccacg
    10801 tccgcgtggt tatgcgcgcc aatgtcgtcc gttaacatat cggcaaccaa aaaatgccgt
    10861 ctgaaacatt tttcagacgg catagtccat aaatcaaatc agtaatacag atgatggttc
    10921 aattaggatc gcttctgcag gcaggtcaac acatcaaagt gttgaccgat accctgtccg
    10981 aaggaaaccc gaactgttcc cgactgtgaa acataggata aatatcaacg ggcatctgct
    11041 tcttttttgc ctgcaaatac gccgaagccg tcttttctat tatcttgatt tttatagtcg
    11101 tatctgcctg ccatttccgc gccatcttga ccataaaatt tcccggaaat atcgccttga
    11161 tcattatccg atttccactt accttgaaat ccacttccat cgatacttgc agtaaattct
    11221 tgcatcccca tacctgaagg atctttggta tcaatttttc ctgttacggt tctctgtgag
    11281 aaatcaacat ttgccgtaaa cgttcctgca gcttgatatg cgctatcatt aaaccaattg
    11341 ttttcgtatg ctttcctaaa atgcagcacc tttccattgt atgttgcatt acccaccggc
    11401 aattgttctg tcggcttacc ctgtacgaaa agagtatatg agccatcttt taatttagcc
    11461 gccccataaa tcaggtgctt atattggttt tgagaaacca ttaccttatc aaaatcactc
    11521 atcaaactgg ggtgattcct acgtgatact tgctcatcag ctttcgttaa gtcaatggtt
    11581 tccccaccaa tcaccaacgc cgtgatttct cccgtaccgt caaaatattt ctcgccaaac
    11641 acgcctgttt taggttggtt atcggcagca ctttgggggg tttggtcttg gggaggatgg
    11701 gcggtcgggt cgtcgccttg caacccctct tccgcatcct gcctgccact tgcttcatca
    11761 gcctgcatcc cgccttgccc cacctcatca gaattttcgg ctttcgacgg cgggtttgca
    11821 tcttcggcat tcatcgcagg ctcagtatgt tcggttgttt cagaaggagg acttgcctgc
    11881 atccccgtat cttcgcttac agcgttttct tccgtaccat cttgagattc tgaagttgaa
    11941 gaaggttgtg ttggatcagt ttctttagct tcttttcctt ctgatgtatc ttctttaggt
    12001 gccggtttga tttcttctcc tgcttctttt ggtgttgact ctgattccac tgttccttca
    12061 ggtgttgatt ctgttgaagt ttccacatgt tggggcgcat cattttgcac tccgccatca
    12121 gcgcctgcct gcgcggtttc cccttctccc tgcgtatctt taggcggtat ttctgttttg
    12181 gattctcttc catccccgcc cgaagcagtg cctgcagtat cagcacccgg ggcagggctt
    12241 accgtatcag cggggggggg gggtaacttt atttgacggc gtttctgagg atttgactac
    12301 aggtgtaccc cctccccctg aaatacaggc ggaaagggcg aggatggatg ccattgcaag
    12361 caggcttggt ttgaaaggaa tcgtcataat tatctcctat ggcatatttt taataaaaaa
    12421 tatgataatt atttatatct gatggtttga aaaatgccgt catacgcggc agaaaatacg
    12481 gttccgcttc acgagccgag gcgaaaccgt aaaatatatc aatgcctgtg cggcatcata
    12541 ccctcggggg cttcggcatc ggctgccaag ccgaaagttt cgcgcaacac gactttgtag
    12601 aatgcaaagg cttcgcgcgc gccttggatg gcttccgctt cggcttcggg agtcaagttc
    12661 aaggcgttca gatgctcgac gaaggcgcgc cagtgtttac cgcgaccgtc gggatggggg
    12721 gcgaggtggc gcgcgccgtg ttcgccgttg taatcgagtt tttgcgcgtg cttgaacaaa
    12781 aacgccgcac ccaaattaga accttcggcg caataaagcc agccgattgc tttgttgccg
    12841 gtttcgtgtg gcaattcttt gtcgaatttg taaggctctt cgcccaagtc tttcaagtct
    12901 tgcgttacgg catcgtatcg cgccatgtat tccagctcgg gaatggcttt gtttaattcg
    12961 gcatctttat agatgtggtc gacggccttg tggaaaacgg attggagttt caaaaattta
    13021 atgtagtttt ctttgctgac aaacggttcg acagacataa cgagattatc cacgctgtcg
    13081 tgaaccgccg tggtatctgc tttcaagcgt ttggcaaacg tcagggcttg attttcggtt
    13141 tcgctcatag cttttccttt gtcggtaagg gataaggatg cggtgcgggc aaaatccgta
    13201 cctgaccgca tacaaaaata ataaacgaga tttattatca catatttttg gaaaaaatat
    13261 catttgcgtg atgtttttaa gcaggtattt tactattctt tacagaatcg ggattttatc
    13321 aaatgggttc ggcagtcggc ggacagccgc tcaaaaaata tttttgccgg acaccaaggg
    13381 tttgttcata ctgccgaacc tgccggtttt gcatcctgat tgggtgcgtt gcttttttcc
    13441 tttataatgc cgccacttat atttgccact ttcccgatga agccgtttgc cgaaaatatc
    13501 ccccacagcc ttcgcggcaa ctgccgcgac gaagccctgc cgccgcatac ggtagattgc
    13561 ccggaatgcg gctgccgcgc ggatgtgccg caattggaca ggggggaggc ggcgttctgc
    13621 ccgcgctgcg gctataagct cttcagggtg ggcaggcatc ctttttccgc cccgcccgct
    13681 tatgcggcga cttcgctgat tttgatggcg tttgcttacg gtatgacgta tatcgaggtc
    13741 gggataccgg gtgcggcatc cgtcctttcg ctgcccgaga tgatgcgcct gatggtgttt
    13801 caggattatg gttttttggc cgaagtgatg tttgtgctga ccttcggcgc gccggttctg
    13861 tttctgctgc tgtgcctgta tgtctatgcc gcgctgatac ggaaacaggc gtatcctgcg
    13921 ctgcgtttgg caacgcgtgt gatggtgcgc ttgagacagg cgatgatggt ggatgtgttt
    13981 tttgtttcca ctttggtggc gtatatcaag ctctcgtctg tggcagaggt tcgcttcgga
    14041 tcggcgtttt atctgatgtt cgcgctgtcg gttatgctga ttcggacttc ggtatcggtt
    14101 ccccagcatt gggtgtattt tcaaatcggg cggctgacgg gggataatgc ggttcagacg
    14161 gcatcagaag gcaaaacctg ttgcagccgc tgcctgtatt tccgcgacag tgccgaatcc
    14221 ccctgcggcg tgtgcggtgc ggaactgtac cgccgacggc cgaaaagtct gagtatttcg
    14281 tcggcgtttc tgacggcggc ggttattttg tattttcctg ccaatatcct gccgattatg
    14341 atttcatcca atcctgccgc cacggaggtc aataccatcc ttaacggcat cgcttatatg
    14401 tgggacgagg gcgacaggct gattgcggcg gttattttca gcgcgagtat tttggtgccg
    14461 gtactgaaga ttgcggcaat gtcggttttg attgcgtccg cccgcttcgc tttgccaacg
    14521 ggcgcaaaga aattgtcgca cctctaccgc atcaccgaag cagtcggccg ctggtcgatg
    14581 attgatattt ttgtgattat tattttgatg tgttcgttcc acacttatgc cgcgcgcgtc
    14641 attccgggca gtgcggcagt ctatttctgc ctggtcgtga ttctgacgat gctgtccgcc
    14701 tattatttcg acccgcgcct gctttgggac aaacgcgctt cagacggcat tgctttcaat
    14761 gaaacggaaa aatatgactg acaacagccc tcctccaaac ggacacgccc aagcacgcgt
    14821 ccgcaaaaac aacaccttgc tctccgccgt ctggctggtc ccgctgattg cgctgattgc
    14881 cggcggctgg ctttgggtta aggaaatccg caacaggggg cctgtggtta cgctcttgat
    14941 ggacagcgcg gaaggcattg aggtcaacaa tacggtcatt aaggtattga gcatcgatgt
    15001 cggacgcgtt acccgaatca aactgcgcga cgaccaaaaa ggcgtggaag taaccgccca
    15061 actcaatgcg gacgtatccg gcctcatccg cagcgatacc cagttttggg tggtcaagcc
    15121 gcgtatcgac caaagcggcg taaccggttt gggtacgctg ctttcgggtt cgtacatcgc
    15181 ttttacaccc ggcaaaagcg acgaggcaaa agacgtgttc caagtgcagg acattccgcc
    15241 cgttaccgcc atcgggcaaa gcgggctgcg cttgaatttg attggtaaaa acgaccgcat
    15301 cctcaacgtc aacagccctg ttttgtatga aaactttatg gtcgggcaag tcgaaagcgc
    15361 gcatttcgac ccgtccgacc aaagcgtgca ttacaccatc ttcatccaaa gccccaacga
    15421 caaactgatt cattccgcca gccgtttctg gctggaaagc ggcatcaata tcgaaaccac
    15481 aggcagcggc atcaaactca attccgcccc tctgcctgcc ctgctgtcgg gcgcgatttc
    15541 atttgattcg ccgaaaacca aaaacagtaa aaacgtcaaa agcgaagaca gcttcacgct
    15601 ttacgacagc cgcagcgaag tcgccaacct gcctgacgac cgctcgctgt actacaccgc
    15661 gtttttcaaa caatccgtgc gcggcctgac cgtcggttcg cccgtcgagt acaaagggct
    15721 gaatgtcggc gtggtttccg atgtccctta tttcgaccgc aacgacagcc tgcatctgtt
    15781 tgaaaacggt tggattcccg tacgcatccg catcgagcct tcccgtttgg aaatcaatgc
    15841 cgacgagcaa agcaaagaac attggaaaca acaatttcag acggccttaa acaaaggcct
    15901 gaccgccacc atctccagca acaacctgct gaccggcagc aaaatgattg agttgaacga
    15961 tcagccttcg tcttcgccca agctgcgacc gcataccgtt tatgcaggcg ataccgttat
    16021 cgcgacccag ggcggcggtt tggacgattt gcaggtcaaa ttggcggatt tgctggacaa
    16081 gttcgacaaa ctgcctttag ataagacggt tgccgaattg aacggttcgc ttgccgagct
    16141 caaatccaca ctcaaatctg ccaatgccgc cctaagctcc atcgacaaac tggtcggcaa
    16201 accgcagaca caaaacattc cgaacgaact gaaccaaacc ctgaaagagt tgcgcacaac
    16261 cctgcaaggc gtatcgcctc aatcgcctat ctacggcgac gtacaaaata cgctgcaaag
    16321 tttggacaaa accttaaaag acgttcaacc cgtcattaac actttgaaag aaaaacccaa
    16381 cgcgctgatt ttcaacagca gcagcaaaga ccctatcccg aaaggaagcc gataatgcgc
    16441 ctcttcccca tcgccgccgc cctgacgctt gccgcctgcg gtactgtgca aagcacacaa
    16501 tatttcgtgt tgcccgacag ccgctacatc cgccctgcaa cgcaaggcgg cgaaaccgcc
    16561 gtcgaagtcc gccttgccga accgctcaaa cgcggcggac tggtctatca aaccgacccc
    16621 taccgcctca acaccgcaca aaaccacgtt tgggcggaca ccttggacga tatgctcgaa
    16681 gcggcgttga gcaatgcatt caaccgtttg gacagcacac gcatctttgt tcctgcctca
    16741 cgcagcggca gtaccgaaaa atggacggtc tatattgacg cattccaagg cagctacacg
    16801 ggcaaaaccc tcatcagcgg ctacgccgtc ctacccgacg gtacgaacag acccttccat
    16861 atcgaaaccg aacagcaggg tgacggctac gccgcgatga ccgccgcact cgaacaggga
    16921 ctgaaacagg cggcgcaaca gatggtcgag taaaccgtga actattgcga atttgccgcc
    16981 tcacttcccg aaaacaccga caacccgaac aaacattacc acgacacgca atacggtttt
    17041 ccgattgagg acgacaatga attgtttgaa cggctggtgt tggaaatcaa tcaggcagga
    17101 ttgagctgga cgctgatgct gaagaagcgg caggcgtttc agacagcatt tgaagggttc
    17161 gacatcgata cggttgccgc atttgacgaa gccgatattg agcggctgct tgccgatgcg
    17221 ggcatcgtcc gcaaccgcct gaaaatcgat gccgccattt tcaatgcacg gcaaatccaa
    17281 gcgttgcaac aagaatacgg ctcgttcaag aactggcttg acgcgcacca tccgcgaagc
    17341 aaagacgaat gggtcaaact ctttaaaaaa catttcaaat tcgtcggcgg cgaaatcgtc
    17401 ggcgaatttc tgatgagtac cggctacctc aaaggcgcgc acgccgaaag ctgtccggtt
    17461 taccgcgaaa ccctgaaata ccacccgaaa tggctcgatg ccgtctgaaa aaccaatgaa
    17521 cagaagaatc ttcctcctcg gcgcaggcgc gttgctgctt accgcctgcg gcagaaaatc
    17581 cgcccgaacc cacgccaaaa ttcccgaagg aagctccgta cttgccttgg gcgattcgct
    17641 taccttcggc tacggcgcaa accctggcga atcctacccc gcgcaactgc aaaaactgac
    17701 gggttggaat attgtcaacg gcggcgtatc gggcgataca tccgcccaag ccctgtcgcg
    17761 cctgcccgcg ctgttggcac gcaaacccaa gcttgtgctt gtcggcatag gcggcaacga
    17821 ctttctgcgc aaagttccca aggagcagac ccgcgccaat atcgcgaaaa tcatcgaaac
    17881 cgtgcagaag gaaaacatcc ccgccgtcct cgtcggcgtg ccgcacatca cactgggcgc
    17941 gttgttcgga catttgagcg atcatccgct gtatgaggat ttgtccgagg aatacggcat
    18001 tccgctgttc ggcggcgcgt gggcggaaat tttgggcgat aataatctga aatccgacca
    18061 aatccacgcc aacggcaaag gctatcggaa atttgccgaa gatttgaatc aatttttgag
    18121 aaaacagggg tttagataaa caaaggttta tccgcaccca agttgtttat ataatcatga
    18181 accgactgga acaccaaact gcttcgggac gcatatgccg tctgaagtgc aaaacctacg
    18241 ccatacagcc gcatgaagtt gcggcggtat ggcgtttttt gaaaaagatg gtctgccggt
    18301 tcagacggca tgaccgaccg tccgaacctg ctgtggataa agcccggaca ggctgaaatc
    18361 atggaatatt gcgaacctga agaagcatcc gacccgtacg caacatacag gcgtgccaac
    18421 ctgatggcgg gtctgccgct gtttgtcgtg attttggttc tgctcaatat tgtttttccg
    18481 cttccggcgt atccattggc ttggctggtg cctgcaggtt tcatggtttt gggcggcggc
    18541 tttcccttat cgctgccgct tgtggcgctg cttgtcctga cctgctgcat tctggcgcat
    18601 tgtccgccat tatcccgtct tttgtgctgc ccttgcccga atcatccgat gtctaaaaat
    18661 tctgcctgat ggcagcccta caaacccgaa ggagtagaaa tgaaactatc tgaattgttc
    18721 aacccgaacg aatttgccgc gcgccatttg agtttcggcg acgaagcggc gttgcttgcc
    18781 gctgtcggcg agaaaagcat ggacgaattt gtcggcaata ccgtgccgca aagcatccgt
    18841 atgccgtccg aactcgattt gcccgatgcc ctgaccgaag cggacgcgtt ggcgaaattg
    18901 aaaggcattg cgtcgaaaaa cgtgatcaac aagtcctata tcggtttggg ttattacccg
    18961 acccgcgtgc cgaatgtgat tttgcgcaac gtattggaaa atccgggctg gtataccgcc
    19021 tacacgccgt atcaggcgga aattgcccag ggtcgtttgg aagctttgtt gaacttccaa
    19081 caagtgtgta tcgatttgac cggtttccct gtggcgggcg cgtctttgct ggacgaagcg
    19141 accgccgccg ccgaagcgat ggcgatggcg caccgcgtgg gcaaggtgaa atccgagcgt
    19201 ttctttgtgg atgcgcgcgt gtatccgcaa actttggacg tgatgaaaac ccgcgccaaa
    19261 tatttcggct tcgagctggt ggtcagcgat tttgccaaag cggatgacgg cgaatacttc
    19321 ggcgcgctgt tccaatacgt cggcaaagac ggcgatgtgc aagacttgca ggatgttatc
    19381 ggtcgtctga aagcaaaagg cacgatcgtt gctgttgccg ccgacatcat gagcttggtt
    19441 ttactgaaat cacctgccga attgggagcg gatattgctt tgggcaacac ccaacgtttc
    19501 ggcgtgccta tgggcttcgg cgggccgcac gccgcttatt tcgcgtttaa agacgcgttc
    19561 aaacgttccg cccctggccg catcatcggc gtatccaaag acgcatcagg caaacctgcc
    19621 ttgcgcatgg cgttgtccac ccgcgagcaa cacatccgcc gcgaaaaagc cacgtccaat
    19681 atttgtaccg cacaagcatt gctggcgaat ttggcgggta tgtatgccgt ttaccacggc
    19741 cctgaaggcg tgaaacgcat cgccaaccgc attcacgcgc tggcttctgc ttttgccgat
    19801 gcgctggttt cagacggcat taatgtggtt cacaaagtct tcttcgatac cgttaccgtc
    19861 gatttcggca acaaagagaa agcagaccaa gtgtttgccg ctgctttgga atcgggctat
    19921 aacctgcgcc gcgttaacga tactcaagtt gcggctgcat tccacgaaac atcggcatgc
    19981 gaagatttgg ttgatttgta ccgcgcgttt accggcaagg atacgttcgc atttgccgat
    20041 gatgtcaaag gccgtctgaa cgccgaactg ctgcgtcaag acgacattct gcaacatccc
    20101 gtgttcaacc gttaccacac cgaacacgaa atgctgcgct acctgaaaaa actcgaagac
    20161 cgcgacttgg cgatgaaccg cagcatgatt tcattgggca gctgcaccat gaaactcaac
    20221 gcgactgcgg aaatgttgcc gattacttgg gcggagttct ccgacatcca tccttacgct
    20281 cctgaagcgc aaaccgccgg ctaccgcgaa ttgctcgccg atatggaaaa cagcctgaaa
    20341 tccatcaccg gttttgatgc aatttccttc cagcccaact ccggcgcgca gggcgaatac
    20401 agcggtatgc ttgccatccg ccgctatcag gaagcgcaag gcgaagcgca gcgcaacatc
    20461 tgtctgattc ccaaatccgc ccacggcacc aaccccgcca ccgccgccat gctcggtttg
    20521 aaagtcgtcg tcgtcgatac cgacgaacac ggcaacgtca acatcgacga tttgaaagcc
    20581 aaagccgagc aacaccgcga cgctttgtcc gccatcatga ttacctatcc gtccacccac
    20641 ggcgtgtacg aagaaggcat ccgcgacatc tgccgcatcg tccatgaaaa cggcggacag
    20701 gtttacatgg acggcgccaa cctcaacgcc caaatcggca tcatgcagcc tgccgaagtc
    20761 ggcgcggacg tgttgcacat gaacctgcac aaaaccttct gtatccctca cggcggcggc
    20821 ggtccgggca tgggtccgat tggcttgaaa gcccatctgg ctccgttcgc accgggacac
    20881 gctttgaccg acacccacag cgccagtgcc gaccaaaccg ccgttgctgc tgcggccttc
    20941 ggttctgcgt ccatactgcc gattacttgg atgtacctga ccatgatggg caaacaaggc
    21001 atggaacagg cgacacgctg ggcattgctc aacgccaact acgttgccaa acgcctgagc
    21061 gaagactatc cgattctgta tacaggcaaa aacggccgcg tcgcgcatga atgtatcgtc
    21121 gatttgcgtc cgctcaaagc cgaaagcggc atcactgaaa ccgacatcgc caaacgcctg
    21181 atggactacg gcttccacgc cccgacggtt tccttccccg ttgccggtac gctgatgatc
    21241 gagccgaccg agagcgaaag caaagccgaa ctcgaccgct tcatcgccgc cctgaaacaa
    21301 atcaaacagg aagtgctgaa agtcgggcgc ggcgaatggc cgaaagagga caatccgctg
    21361 gtcaacgctc cgcataccgc atccgatgta accggcgaat gggcgcatcc gtactcccgt
    21421 gaagaagccg tctttccgtt gccattcgtc cgcgaacaca aattctggcc gagcgtcaac
    21481 cgtgtggacg acgtgtacgg cgaccgcaat ctcgtgtgca gctgcccacc gatggaaaat
    21541 tatgaagact gaccgttgaa accccgaaga caaatgccgt ctgaaacatt ttcagacggc
    21601 attttcgtca acggcagact ggttgcacca atacacgtat ctcgactata actttaaaac
    21661 aaatgagtta aaccagtatc catacatcag cttttttatc atcctacttt ttattcatcc
    21721 gatcgtgcaa acagatttca aagatgaaaa gcctattcac accctttgat gtcatttcca
    21781 cacggacaaa caaatatagt ggattaacaa aaaccagtac agcgttgcct ctccttagct
    21841 caaagagaac gattctctaa ggtgctcaag caccaagtga atcggttccg tactatctgt
    21901 actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactataa attcccataa
    21961 aaaaacggag cagatacctg ccccgttttt atttaatccg aaattttaat ctaaatttag
    22021 aattttgcac cggattggtt tgccatatag tcaacagccg ctttgacttc gtcatcgctc
    22081 aaaccggcat tgccgccttt ggccggcatc gcgttaaagc cttcaagggc gtgtttgtgc
    22141 aaggtttctt tgcctttttt gatacgcggt gcccaatcgt cttttttgcc tatgccggga
    22201 ataccgggaa tcgaaccgcc gtggcacacc tgacaggttg cttcgaagac ttttttaccg
    22261 tcaacgccga ccgcaggggc tgccgcaccc ttgtcttctg ccttcgcttc tgccggagct
    22321 gcactatcgg caggagcaga agctgttcct gaagcggcat tgtcggcagg cgcagcctca
    22381 tcaggattcg ggaaagaacc gccgcttttg ttcgccatgt aagtaatcgc ccgtttgagt
    22441 tcctgatcgg tcaggtctgc cgcaccgcct ttggcaggca tggcgttaaa gccgttcagc
    22501 gcgtgttgga acaaggtatc gaagccttgc gcgatacgcg gtgcccaatc gccgttgtgt
    22561 tccagtttcg gagcgttcgg cacattgctg tccgccgcgt ggcattggat acagattttg
    22621 ccgaaaatct gttcgccttg gcgttcgccg acggggatgc cgtcgcccat cgtcaattgt
    22681 ccgacaggct ggatacgggt ctgcgttgct gcttccgtag tggcatcgac atcgccgaac
    22741 gagccgctgc ccgccagctt aatcaggaaa taaaggactg caataacaat aacgataccg
    22801 ctcacaaggg taaacagtgc agagccttgg gctttgttgt cgcggagttg tttcatttgg
    22861 taggcctcgc cgtcaggtta ggttgtgctg taaattatag tttggtgtgt taaacgcagt
    22921 taacaatatt ttgctggatt atactgaatt cacagggtct ttccaatcgc tatcattgaa
    22981 aatatgaaaa aatttgccaa cggtatctgt ataaaacaaa taatcctttg aaaataatgg
    23041 tttatcctca agaaaactct ccttatgccg ccatacgccg cctgccggcg caagataacc
    23101 tttgccaatt tgcagaattt acgttaacct tacgttttac gcacccatag ctcagttgga
    23161 agagtgtcag tttccgaagc tggaggtcac aggttcgatc cctgttgggt gcgccaatta
    23221 taaagagacc gtctgaaaga taaatatttt tcagacagtc ttttgactta cttcaaactc
    23281 ttatttcaag acttccgcaa atgcgcgggc aacatagtcg gtattcgacg tattcagtcc
    23341 ggcgacgcac atcctgccgg aatccagcag gtaaacggca aattcgtcgc gcagcctgcg
    23401 gacttgttcc acgctcaatc ctgtgtagcc gaacatgccg cgctgtttga tgaaataagt
    23461 gaaatcgcga tcggggattt gcgcagttaa gacatcataa agtttctgcc gcatcgcacg
    23521 gatgcggtcg cgcatcatat aaacctcgtt ttgccacaag gcgtaaagtt cggggctgtt
    23581 catcacgtcg gcggcgatat acgcgccgtg cgcgggcggg ctggagtaga tgcggcggac
    23641 ggtgaatttg agctgtccga acaccaaatc cgcttcttcc ttattcgggc aaaccacgct
    23701 taagccgccg acgcgctcgc cgtagagcga caggtttttt gagaaggaat tgctgacgaa
    23761 caagggcaat tccatttcca ccgctttgcg gacggcgtag gcatcgctgt ccaaatcgcc
    23821 gccgaagcct tggtaggcga tgtccataaa cggaatcagt ttgcgcgttt tgatgatgtg
    23881 caacacttcg tcccattgtt gttccgacat atccacgccg gtcgggttgt ggcagcaggg
    23941 atggaggatc aggacgctgt tttcgggcag ggtgttgaaa aacgcggtca tttcgtcgaa
    24001 tttcacgccg acagtggcag ggtcgtaata tgggtaagtg ccgacctcga aacctgcgcc
    24061 ttcaaaaatg ccgcgatggt tgtcccaagt cgggtcgctg acgtaggcgc gcgcttcagg
    24121 aaaccagcgg tgcaggaaat ctgctccgac tttgagcgcg cccgagccgc ccaaggtttg
    24181 tacggtagcg atgcgtcctt gcgcgagcgc agggttgtct ttgccgaaca ataaatgctg
    24241 caccgcgctg cggtaagtgt ccaagccttc catcggcagg tagggcgacg gcgcaggcgc
    24301 ggcggcacgg gcggtttcgg ctcggctcac ggattccaac acgggcattt tgccttcgtc
    24361 gtcgaaataa atgcctatgc tcaaattgac tttttcgggg cgcgggtcgt ttttgaaggt
    24421 ttcgaccaaa ctcaaaatcg ggtcgccggg atagtattcg atgtgtcggt acatagtcct
    24481 tacctcttgc tttttcaaag gattttcttt ttcaacaata caccactttc gatatggtgc
    24541 gtaaacggga attggtcgaa cagggcggcg cgttcgacca catgggtttc caccaaggtg
    24601 tccaaattgg cacgcagcgt ttcgggattg caggaaatgt agatgatgtt gtcaaactgc
    24661 gacaccagct tcaaagtttc ctcatcgata ccggcacgcg gcggatcgac gaaaatagtg
    24721 gaaaatgcgt aatccgtcaa agcaataccg ccatccttaa ggcgtgcaaa ctcacgtttg
    24781 ccggtatagg cttcggtaaa ttcctcggca gacagacggg cgattttgat gttgcctatc
    24841 ccgtttgcct caatattcca ttgcgccgca cctactgaag ttttggaaat ctcggttgcc
    24901 aaaacacgtt caaacttttc agataaaggc agggtgaaat tgccgttgcc gcaatacagt
    24961 tcgagcaggt cgccgcccaa gccttccgcc gcaccgcatg cccattcaag cattttttga
    25021 cacacggcgg cattgggttg ggtaaaaccg ccttcaattt gccgataacg aaaatcccga
    25081 ttaccgacct ttaaagtttc cgttacatag tcctgtttca agactatttt ctgccccctg
    25141 ctccgcccaa taacggaagt atccaactgt tgctgtaacg cttgcgccgc ctgcatccac
    25201 tcatcatcaa gccttttgtg gtaaatcatg gtaaccagca tttccccgct gagcgtagac
    25261 aaaaattcga cggcatacca acggtttctc agttcggcag attgggcggc aacggcaatc
    25321 aattctggca tcaggcggtt gacggcttcg gaagccgctt caaaacggtc gcaacggatc
    25381 aggcttgccc cgccagcctt ttgtcctttt tcaaacatgg cataaaacat ttcaccgcct
    25441 tcgtgccaaa tacggaactc ggcacgcata cggtaatgtt tgtccggaga ttcgtacact
    25501 tcccactcag gaacatccaa acctgcaaaa agggttttaa ggtggtcttt tttatcttga
    25561 agctgctgcg tgtagtcgct catatcgcta tcctgaaaaa tcagaccgga ttataagccg
    25621 atttgcctcg ccgcacgcaa actctttgac tgccgcccct tcaatgacgg acgggctttt
    25681 gtgctaaaat ccgccatctt tccacactat accgataaag ggaaaaatca tggcaggcaa
    25741 cactttcgga caactcttca ccgttaccac cttcggcgaa agccacggcg cgggtttggg
    25801 ctgtatcatc gacggctgcc cgcccggctt ggaattaagc gaagcggata tccaatttga
    25861 cctcgaccga cgcaaacccg gcaccagccg ccacgttacc caacgccgcg aagccgacca
    25921 agtcgaaatc ctctccggcg tattcgaagg caaaaccacc ggcacgccca tcgccctctt
    25981 aatccgcaat accgaccagc gcagcaaaga ctacggcaac atcgccacca gcttccgccc
    26041 cggccacgcc gactatacct attggcacaa atacggcacg cgcgactacc ggggcggcgg
    26101 caggagttcc gcccgcgaaa ccgccgcccg cgttgccgcc ggagccgttg ccaaaaaatg
    26161 gttgaaagaa aaattcggca cggaaatcac cgcctacgtt acccaagtcg gcgaaaaaga
    26221 aatccggttt gaaggctgcg aacacatttc ccaaaatcct ttttttgccg ccaaccatag
    26281 ccaaattgcc gagctggaaa actatatgga cagcgtgcgc aaatccttgg attccgtcgg
    26341 cgcgaagctg catattgaag ccgccaacgt ccccgtcggc ttgggcgaac ccgtattcga
    26401 ccgcctcgat gccgaaatcg cctacgccat gatgggcatc aacgccgtca aaggcgtgga
    26461 aatcggcgca ggttttgaca gcgtaacgca acgcggcagc gaacacggcg acgaactcac
    26521 cccgcaaggc ttcctgtcca accactcagg cggcatcctc ggcggcatca gcaccggaca
    26581 agacatccac gtcaatatcg ccatcaaacc caccagctcc atcgccaccc cgcgccgcag
    26641 tatcgacatc aacggcaacc ccatcgaact cgccacgcac ggcaggcacg acccctgcgt
    26701 cggcttgcgc gccgcgccga ttgcagaagc tatgctggca ttggttttaa tcgaccacgc
    26761 cctgcgccat cgcgcgcaaa atgcggatgt tcaggttaat acgcccgaca ttaccctttc
    26821 aaacaaataa aaatttagcc aaaacacaga ctttataata gaataccgag cattgccgtg
    26881 ccgctttgac gcacgggttt gtttagagga atacaaccga aatgacacaa gaaaccgctt
    26941 tgggcgcggc actgaaatcc gccgtccaaa ctatgagcaa aaagaaacag accgaaatga
    27001 tcgccgacca catctacggc aaatacgatg tattcaaacg cttcaaaccg ttggcgctcg
    27061 gcattgatca ggatttgatt gccgctttgc cgcaatacga ttccgcactg attgcacgcg
    27121 tcctcgccaa ccactgccgc cgtccgcgtt atctgaaagc cttggcgcgc ggaggcaaac
    27181 gtttcgattt gaacaaccgt ttcaaaggcg aagttacccc cgaagaacag gcgatcgcgc
    27241 aaaaccatcc tttcgtgcag caggctttgc aacagcagtc cgcccaagct gccgccgaag
    27301 cagccggatc ttccgcagca gaataaatcc ccaaacgaaa tgccgtctga aaaccgattt
    27361 ggtttcagac ggcatttttt cgtatgcggc aatcacggtt caaatatcca attccgccgt
    27421 atcgccttcg cgttccatcc aagcgcggcg ggcggcggct tcgcctttgc ccatcagttt
    27481 gacgaagatg tcgcgtgttt cgtcatctgc gccttctggg atttgtacct gcaacagacg
    27541 gcgggtgtcg gggtgcatgg tggtgtcttt gagttggtcg gggttcatct cgcccaagcc
    27601 tttaaaacgg ctgatggaat aagcagtttc tttaaagcct tctttttgta gtcgctccaa
    27661 aatgctgtcg agttcgtttt ggtcgagggc gtagaatttg cgggccggtt tgtttttgcc
    27721 ttgtgcattg acatcgacgc ggaacagcgg cggctgggcg acgtagatgt gtccatcggc
    27781 gaccagtttc gggaagtggc ggtagaacaa ggtcagcagc aaaacttgaa tatgcgagcc
    27841 gtccacgtcg gcatcggaca ggatggcgat tttgccgtag cgcaggccgc ttaaatcggg
    27901 atggtcgtta ataccgtgcg gatcgacgcc gatggcgacg gaaatgtcgt ggatttcggc
    27961 gttgccgaag agttggtcgg ggtggacttc aaagctgttg agcactttgc cgcgcagggg
    28021 caggatggct tgggtggctt tgtcgcgggc gagtttggct gagccgccgg cggaatcgcc
    28081 ttcgacgagg aagagttcgt tttcgcggat gtcttcgctt tcgcagtcgg tcagtttgcc
    28141 gggcaggacg gcgacgccgc tgcctttttt cttttcgatt ttcttaaccg aacgcatccg
    28201 cgcctgcgcc tgacggatgg cgagttcggc gattttttta ccgaagtcca cgttttggtt
    28261 cagccacaat tccaaagggt cgcccgatac ggcggcaacg agtttcaacg cgtcgcggtt
    28321 ggtcagcttg tctttggttt gaccttggaa ctgcgggtcg aggacgcggg cggagaggac
    28381 gaaggcggtt ttaccgaaca cgtcgtcact ttgtactttc acgccgcgcg gtaggaggtt
    28441 gtgcagattg atgaaattgt tgacggcgtt gaacacggct tgtttcaaac ctgcttcgtg
    28501 cgtgccgccc agcggggtgg ggatgaggtt gacgtagctt tcgttggcgc acgagccttc
    28561 ttccagccag gtcagggcaa acgccgctcc ttcgccgatg ctgaaatcgc cgttgtggtc
    28621 gtctgaaatg tagttttcgc aagagaagat aggcacggct tcctgcgcgt cggcaatcag
    28681 gtcggtcaga tagcttttca gaccgtcggg gtaatgccag gtttgggtgt gcgcttcgtc
    28741 ttcgcctttg accggacggg tcagggaaac gcgcacgccc ggcagcagca cggctttggc
    28801 gcgcagcagg cgttcgagtt cggggatgct gtaattcggg ctttcaaaat atttgccgtc
    28861 cggccagacg cgcacttcga tgccgctgtc tttgacggcg catttgccca cttgtgccaa
    28921 cggttcgacc acgtcgccgc cggcaaacac gatgcggtgg attttgcctt cgcgtttgac
    28981 cgttacttca aggcgggtgg aaagggcgtt ggtgacggat acgcccacgc cgtgcaggcc
    29041 gcctgaaaag gcatacgcgc tgccgccgtc ttttttgttg aacttgccgc ccgcgtgcag
    29101 acgggtgaat acgagttcga ctacggatac gccttcttcg ggatgcaggc cgacgggaat
    29161 gccgcgccca ttgtcgtgca cggaaagcga accgtcttca tgaatttgca cgtcgattgc
    29221 agtcgcgaaa ccgcccaacg cttcatccgc cgcgttgtcg atgacttctt ggcagatatg
    29281 ggtcgggctg tcagtgcggg tgtacatgcc gggacgttct ttgaccggct ccaagccttt
    29341 gaggacggtg atgctggatt cgctgtattg gttgttttta gccataggaa taatctgaaa
    29401 gtaagaaaaa caacgctttc agacggcctg aaagcgttgc gttccgttgt tttaccggtt
    29461 gccggaaggt tggcgggcgg caaagtcttc ataactttcc ataccgcgca ggaagcggga
    29521 agagagttct ttcaacgccc ccaaataaac gtcgcgtttg aactcgatga cttcgtcaac
    29581 gggcgcccaa tattgatgcc aacgccagcc gtcaaattcg gggtggcggg tggcgcgcag
    29641 gttgacatcg caatcccggc cggtcaggcg caggagatac caaatctgct tctgcccgcg
    29701 ataagagccg cgccattcgc ggcgcaccca gttgttcggc acgtcgtaac gcagccagtc
    29761 gcgcgtccgc ccgacgattt tgacgtgttg cggcagaagt ccgacttctt cgtaaagctc
    29821 gcggtacatc gcggtttcgg ggctttcgcc cggcttgatg ccgccttgcg gaaactgcca
    29881 agaatgttcg cgcacgcgct taccccaaaa gacttcgtcg cggttgttgg ttaagatgat
    29941 accgacattg gggcgatagc cttctctgtc caacacggtg tcgccctccg ttaaattcaa
    30001 tcttgggatt ttcccacaaa tcgaacggtt ttgacaaatc agacggtatg gttcaagcat
    30061 gtcgaaacac agacggactc gggaaaatat cttaaattta gtttacaata atacgtttca
    30121 aattattcgg gaatcagacc atgttagata tccaattgct ccgcagcaac accgccgccg
    30181 ttgccgaacg gcttgcacgg cgcggttatg actttgatac cgcacgtttt gacgcactgg
    30241 aagaacgacg caagtccgtt caggtgaaaa ctgaagaact acaagcctcg cgcaacagca
    30301 tttccaaaca aatcggcgca ctgaaaggtc agggcaaaca cgaagaagcg caggcggcga
    30361 tggatcaagt cgcccaaatc aaaaccgatt tggaacaggc tgccgccgat ttggatgccg
    30421 ttcaaaaaga attggacgca tggttgttga gcatacctaa cctgccgcac gaaagcgtac
    30481 ctgccggtaa agacgaaacc gaaaacgtcg aagtccgcaa agtcggcacc ccgcgcgaat
    30541 ttgactttga aatcaaagac catgtcgatt tgggcgaacc tttgggtttg gatttcgaag
    30601 gcggcgcaaa actctccggc gcacgattta ccgttatgcg cggacaaatc gcccgtctgc
    30661 accgcgcgct ggcacaattc atgctcgata cgcacacact gaaacacggc tacaccgagc
    30721 attacacgcc ctatattgtg gacgacacca ccctgcaagg tacgggccag ctgccgaagt
    30781 ttgcggaaga tttgttccac gttacccgtg gcggcgacga gagcaaaacc acgcaatatc
    30841 tgattccgac agctgaagtt accctgacca ataccgttgc aggcagcatt ttagacgaag
    30901 gcggactgcc gctgaaactg accgcccact cgccctgctt ccgcagcgag gcgggttcgt
    30961 acggcaaaga cacgcgcggt ctgattcgcc agcaccagtt cgacaaagtg gaaatggttc
    31021 aaatcgttca tcccgaaaaa tcatacgaaa cgctggaaga aatggtcggc catgccgaaa
    31081 acatcctgaa ggctttggaa ctgccctacc gcgtgattac cctgtgtacc ggcgacatgg
    31141 gcttcggcgc aaccaaaacg tatgacttgg aagtttgggt accggcgcaa aacacctacc
    31201 gcgaaatctc aagctgctcc aactgcgaag atttccaagc ccgccgcatg aaggcgcgtt
    31261 tcaaagacga aaacggcaaa aaccgcttgg tacatacttt gaacggctcc ggcttggctg
    31321 tcggcagaac gctggtcgcc gtattggaaa accatcaaaa cgccgacggc agcatcaaca
    31381 tccctgccgc actgcaaccg tatatgggcg gcgttgccaa gttggaagtg aaataagttt
    31441 gcaagctgcc tgaacgtcaa atgccgtctg aaacctggtt cagacggcat ttcctttaaa
    31501 cttttaaaac acatcaaccg tcggcacgaa ccgcattgcc gcaatcgccg gtctgtccga
    31561 cctcgcggat attggacagc gtaacttccg aaatattacc caacgcctct tccgtcaaaa
    31621 atgcctgatg gccggtaaac agcacattat gacaagacga caggcggcgg aacacgtcgt
    31681 cggtaatcac atcgttggat ttgtcttcaa aaaacagctc gcgctcgttc tcgtacacat
    31741 ccatgcccaa tgcgccgatt ttccggcgtt tcaacgcctc aatcgcggcg gcactgtcaa
    31801 tcagcccgcc acggctggtg ttgataatca tcacgccgtc tttcattttg tcgaacgccg
    31861 cttcgttcag catatagtgg ttttccggcg tggcggggca atgcagcgtg atgatgtccg
    31921 accgggcata cagctcgttc aactccacat acctgccgcc gattttttcc gcttcggggt
    31981 tgcaaaacgg atcgtaagcc agcaggttca tgccgaaacc ctttaaaatc cgcatcgttg
    32041 cgataccgat tttccccgtg ccgataacgc ccgccgtttt gccgtacata ttgaaaccgg
    32101 tcagaccttc cagcgaaaaa ttcgcatcgc gggtacgctg ataggctttg tggatacggc
    32161 ggttcaacgt cagcatcaga ccgaccgtat gttccgcaac cgattcgggc gaataggcag
    32221 gcacgcgcac gactttcaag cccaactctt cagccgcttt caaatccaca ttattgaagc
    32281 cggcacaacg caacgccaca gttttcacgc caatttgcgc caatttttcc aacacgggcc
    32341 ggctgccgtc gtcgtttaca aaaatacaaa ccgcttccgc gccttccgcc attttcgccg
    32401 ttttcgcatc cagcataaaa tcaaaaaact ccagctcgaa gccaaaatgc cggttggcgc
    32461 gggtaaaatg ttcgcggtca tagcttttcg taccgtaaat cgcaatcttc atcaatatgt
    32521 ccagttgtcg tctatggttg agaaacggca taatacactg aattcaaaca aatcagtaga
    32581 atatggtgga ttaaaattga ttgcatgcac ggcatttcca tttcaaaaca caaaactcaa
    32641 tcgcccattg ccgccaaaag ctccgcctga tgttcggcaa tcagggcatc ggtgatttct
    32701 gccaaatcgc cgtccatcac aaaatccagc ttgtgcaggg tgaggttgat gcggtggtct
    32761 gttaccctgc cttggggata gttgtaggtg cggatgcgtt cgctgcggtc gccgctgccg
    32821 atgagggatt tgcgttcggc ggcttctttg gcttgggctt cgcgtttttg cgcgtcgttc
    32881 aggcgggcgg cgagaacttt cattgcctgc gctttgttgg cgtgttggct gcggccgtct
    32941 tggcattcga ccaccatgcc ggtgggcagg tgggtaatgc ggacggcgga atcggttttg
    33001 ttgatgtgct gaccgcctgc gccggatgcg cggaaggtgt cgatgcgaag gtcgacgggg
    33061 ttcaactcga tgtcttccag ttcgtccgct tcgggcatga cggcaacggt gcaggcggag
    33121 gtgtggatgc ggccttggct ttcggtggcg gggacgcgct gcacgcggtg tccgcccgat
    33181 tcaaatttca aacggctgta cgcgcctaag ccgataatac gggcgataac ttctttatag
    33241 ccgcccaact cgctttcgtt ggcggacacg atttcaacct gccagcggtt gcgttcggcg
    33301 tagcggctgt acatacgcag caaatcgcca gcaaacagcg cggcttcgtc gccgcccgtt
    33361 ccggcgcgta cttcgataaa gatgtttttg tcgtcgtcgg catctttggg cagcagcagt
    33421 ttttgcaatt cggtatcgag tgcgccgatt ttggctttcg ccgcttcgat ttcttcggcg
    33481 gcaaagtctt tcatttcggg gtcggacaac atttcttcgg catccgccaa atcgctttgc
    33541 gccagttgat agttttggaa tacttcgacg acgggggtca gttcagcgtg ttcgcgcgtg
    33601 agcttgcggt agttgtccat atcggacgtg gcttcgggtt gccctaaaag gtgggtaact
    33661 tcttccaacc ggtcgccgag ttgttgcagt ttttctaaaa tagacggctt cataattctt
    33721 ccataacaaa cgccgcctga agttcagacg gcatcaaaac gcggtcatta taatatattt
    33781 tctgaatatt caaaaagata atctagggac ggagcaggct gtcggcattg cgttccgtta
    33841 tcgcggcgat gtccgccacg ccgacaccgc gtatctgcgc ggcaatttcg gcaatccaaa
    33901 gaatattggc aggcgtatta atctcttttt tcagcataaa cgggctgtcg gtttccaata
    33961 cgaaatcccc gtcgttcaag gctttaagcg tatcgcgcac tttacgcgcg ttcggattga
    34021 gcaacaacga accgatgccg attttgaaac ccagtttcgt caacacgcgc gcttcttccg
    34081 cgctgcctga gaaggcgtga acgatgccgc cttgggcaaa gcctgtctgt ttgacggcgg
    34141 cggcgatgtc ggcggtggct ttgagattat ggataatcac gcggcggcgc agggtttgcg
    34201 cgatggcaag ctggcggacg aaaacttgga tttgccgttc gcgctgcggc ggcgtttggg
    34261 ttttgtcgta aaaatccaag ccgatttcgc cgacccatgc ctgcggataa cgtgccaaca
    34321 tcgcttccag gcggacgcaa tcacgctcgg caatgccgtc tgaaaaccaa ggatggatgc
    34381 cgagcgcaat acggatttga ccgtgttcgg acggcatttc cgacagttcc gccacgcttt
    34441 gccaatcctg cggacaggtg gcgggaacga taaaccgctt caccccaact ttccgcgcgg
    34501 cggtcaggat gtgcggcagg ttttcgcgca gggcggcatc ggcaaggtgg cagtgggtgt
    34561 cggtgaagtt catttcgatt ccgcaatgcg gcaggttcac ataaaaataa ggcagggcag
    34621 caagggatgc cgtgtacggg cggcgcataa ggcgcggtca acgccccgtc attgccattt
    34681 caatccgcta taacttcagt cttaccaact ctttttccac ctcttcctta ccccagcctt
    34741 gcgatacggc gagggtcata agcgcggtgg cggtttcaag gttgcatttg ccgccgttga
    34801 tgatgcccga gttgcggaac gcattgcctt gcgcgtaaac ggcggcggtt ttgccttgtc
    34861 ggacttggct gatgttgagc aacaatttgc cttgctgcgt gaaatcttgc acggcgcgga
    34921 taaaaccttc gtctgcgggg gtgttgccgt gtccgtagct ttgcaggata agggcttggg
    34981 cgggaagctg tccgagtccg tccgcaagtt cttggacggc aaagccgggg ataagcgtgc
    35041 ggacggcgat tttcgcctgc gggtcgggat aacggatttt gaggccgtct gaaacatctg
    35101 ccgcgtcctg attcgggatg cggacattgt gccagccctg ggtttcgtcc cactccgcca
    35161 gtgcgccgaa atgcggattg tcgaaaccgg cggcggtttc ggtgctgact ttgctgctgc
    35221 cgacggcggg ataaagtttg ccgtcaaagg caataagcgt ctgcttcaat ccgagtggga
    35281 atgcggcaac ggcagtcgcc agattgcgcg gcgcgtcgct gcccacggcg ttgtacggcc
    35341 attgggagcc ggtcaggacg attggcttgt ccaaaccctg cagggcgagg gcgaacagat
    35401 tggcggtata ggcgagcgtg tccgtgccgt gcagcaccaa aacgccgtcg tactgcggca
    35461 gcgcggcggc aatgatgtcc agccaagcgc accagttttg cagttggacg gcggaagaat
    35521 caatcagcgg cccacaaacg tgccaatcga aatccatgcc gtttgaaaac ggcgcgagtg
    35581 cctgcccgac cagcgcggta tcggggcgca ggccttcgct gctttgcgtc atgccgatgg
    35641 tgccgccggt ataaaggaca aaaattttct gtttcatggc tcatcctgaa aatgcaaaga
    35701 ccgcctgaaa cgcgaaatgc gtccggacgg tcgaaaatct gatttactgc tgcttctgtg
    35761 ccgctttttt cgctgccttt tccgccttgc gtttggcgcg tttttcgctc aattgtgcgc
    35821 ggtaaagctg ataatgtcgg cgtttgcgcc accagaacca cgccagcgcc gccgccagca
    35881 cgcccaatgc gatgaagatg ccggattgca ggctgtgcat tttcgccatc agccaatcga
    35941 tgttgtgcgc gccgtactcg cccaagtaaa tccaaacggg cacggaaatc agcgcggcaa
    36001 gcccgtccat aatcagaaag cgcagatacg atactttgcg gctgatgccg gcggtaacga
    36061 aaacggcagt ccgcaaaccc ggcaggaagc gggcgacaaa cagaacccag ttgccgtatt
    36121 tgtcgaattt ttcctgaacc tgtgcgtaac gtttcggcgt catgatgcgc gcaataggtt
    36181 tgaacttgag gattttctgc ccccagatgc gtccggcggc gaacatgatg ccgtccccga
    36241 ccaatacgcc gagcataccg actgcaaaca taatatgcgg attggtataa cccatacccg
    36301 aaatcacgcc gcctgttacc aaggtcaaat cctcgggaat cggcacgccg aaaccgcaga
    36361 tgaccaaaac gaaaaacacg gccgcatagc cgtattcgac aaaaaaggct tccaaaaggg
    36421 caaacatagc ggatattcca ttgtcggaga taaaaagtca gaacaagccg aaacattttc
    36481 tgcgcgaaac ggcattctat caaagattat gccgtctgaa agcggcaagg ggcagattcg
    36541 cgccctctgc cggacgttca gacggcattt aaacaggaaa accgccgctg ttgaaacggt
    36601 tttgcctgat tttgcctaaa ggctgccgat ggcggaagca atacgttccg caccgtcgcg
    36661 cgcccaatcc gcctgccgcg cctcaaccat cactcgcacg acgggttcgg ttcccgaagc
    36721 gcgcaacacg acacgccctt tgccttcgag ttctttttcc acttccgcca acacgtcttt
    36781 tgaggcttcc tgccatttct gacctttttg gatgcgcacg ttaatcatag tttgcggata
    36841 cggctgccaa tcggcgcaaa cggtggcgag gtcttggttc agcgtttgca gtgccgccaa
    36901 aacttgcagc gcggaaataa tgccgtcgcc ggtgttgtgt ttgtccatgc acaaaatatg
    36961 gccgctggct tcgccgccga tgagccagcc gcgttggttc agctgttcca acacatagcg
    37021 gtcgccgact ttggcgcggc agaaatcgac gccctgctct ttcagggcga tttccatcgc
    37081 catattggtc atgaccgtgc cgaccacgcc gccgatgttg ataccttctc gggcgcgggc
    37141 tttagcaatg acgtaaatca ggctgtcgcc gtcgtaaacc ttaccgtttt tatcgaccat
    37201 catcaggcgg tctccgtcgc cgtctaaggc gatgccgtag tcggcttcat gctgtaaaac
    37261 ggctgcctgg agtgtcttgg tataagtcgc accgcatttt tcattgatat tgtagccgtt
    37321 gggttcgtcg ccgatgctga cgacttgtgc gcccagttcg tgaaacacct tgggtgcgac
    37381 accgtacccc gcgccgttgg cagtatcgat aaccaacttc aaaccccgaa ggtcggaatg
    37441 gctgggaaag gtagatttgc aaaattcgat atagcggtcg tccgcaccac tgatgcggcg
    37501 tgcgcgaccg aggcgtgcag aaggctgggt tttcatttct tcgtcgattt tggcttcgat
    37561 ttccaactcg atttcatcgc tcagtttcac gccgccctcg gcaaagaatt tgatgccgtt
    37621 gtcggaatag gcgttgtgcg acgcggaaat catcacgccg gcggacaggc gcaacgcgcg
    37681 ggtcagataa gccacgccgg gcgtgggcag cggtccggtc tgtaccacat tcacacccgc
    37741 cgccgtaaaa ccggccacca aagcggcttc cagcatataa ccggaaatac gcgtatcctt
    37801 gccgattaaa acggtcggtt tctggtcagt gtcgtgctga accaaaacct gacccgccgc
    37861 atagccgagt ttcaatacaa aatcgggcgt aatcggaaac tgcccgactt cgccgcgcac
    37921 gccgtccgtg ccgaaatatt tttttgccat gtgttgctcc gagaatgtga accgttgtcc
    37981 gagattatac agtcagtttg tgccttgctg tctgcaccgt tgatgccgtc tgaaaccgcc
    38041 ccgtcctttt cagacggcat gaagtatgtg aaccgctgtt tacagattga tgcccaacgc
    38101 ttcccacacc ttcaacgcat ccgctgtcgc cttcacatca tgcacccgca cgatttgcgc
    38161 gccgcgcgct acggaagcca acgctgccgc cacgctgccg tgtacgcgtt ccgccgcatt
    38221 tgcctcgccg gtcagctcgc ctatcgtgct tttgcgcgat acgccgatga gcagcggaaa
    38281 acctgtttcc gccatcaatt cgggcaaatg ccgcatcagc gcgatattgt gttgcaaggg
    38341 tttgccgaag ccggagccga agccggggtc gagtatgatg cgttgcggtg cgatgcctgc
    38401 cgcgatacat tccgctgagc gcgctttcaa ataccgcgct acttcaccga caacatcttg
    38461 atatttcgga ttaatctgca tggttttggg caaaccctgc atgtgcatca ggcaaatgcc
    38521 cgtgtccgcc tgacgcgcca gcaattcgac cgcgccctcg tcattcaacg ccgccacatc
    38581 attaataata tcgacgccgc cgagtgccaa cgctttttcc ataatcaccg tgcggcgcgt
    38641 gtccaaactg atgggaacgc cccaccccgc cacttccgcc aaaacaggct caacccgcgc
    38701 ccattcttct tcaggcgaaa cataatccgc acccgaccgc gtcgattcgc cgccgatgtc
    38761 gagaatgtcc gcaccctctt tcaaaagccg ttcggcgtgc gccaaagctg tttgggcatt
    38821 ttgcgaatac gcgccgccgt cggaaaaaga atcgggcgtg agattcacga tacccatgat
    38881 tttcggtttg tccaaaccga tttcaaaccg tcctgcctgc caaacgtgtc gtgccatctg
    38941 aactcctccc aaaataaaaa acagattata tgccgtctga aaccgtcttg tgcgcttcag
    39001 acggcaccgc tattcaggcg gcagacggca tgttgtccga atgtctgctc cgcctttgaa
    39061 tctgccggta tgcctgctat ccgcccgact tttcaaaaca ggttccgacg attccgcacg
    39121 cgcctgccgc ctttgccaag ccgtacagga tttcctgcgg catatcgcgg ttccataatc
    39181 ccgtaatatt cgcaatcacg ggcagatggc tgatttggcg gactttcacg atggattcga
    39241 catccaaacg gtagggatgg tctttggtat ggctcaacac gcccgactcg cccagaatca
    39301 ggtggcggtt gccgcgcgag acaacatatt ctgcggcatt caaccaatct tcggcagaat
    39361 ggtgcttgtc tttacacagc accaagggaa tgttcaggct tccggcttca ttcaatacgg
    39421 caagatcgga catcagcccg ccgcccaaat acaggatgtc cgcccccgca ttcaaagccg
    39481 cttcgacatg gcggacgttg cggacgcgca ccaatacggg tttccctgcg ccatgcgccg
    39541 atgcggtctg ttccgccaac cgtctgcacc gtcctcgccc ttcatccgca cttgaagtgt
    39601 cgtataagtt tgccgaagtg aaaaacggat ctagaaacac tgcatccgca ttgcgccata
    39661 ctgacggttc tgcggcgata cagacggttt ccccgccgcc gaaagccacg cctttggcgg
    39721 caacgcggct gtcttccgcc cgattttccc gactgacggt tttccatgta tccaaaatgc
    39781 ggacggcttt ctcgacctcc ggcagcgtct gcacctccct gacgctcaaa accctatcgt
    39841 cgccgattgc gccgatgacg gtacgctcgt cgccgtgaga aatgtgttct cgcagacctc
    39901 tgctgcggat aaaggcgaca acgccggcaa tgtccgcttc ggcggcacgc ctgctcatga
    39961 caataatcat atttcctcct gacacaagaa acggcctgcc caaaatagga tttttgcaag
    40021 ccgtgttata ctgtggcgtg ttttacagat tgttcgggct atggatttat tatcggtttt
    40081 ccacaaatac cgtctgaaat atgcggtggc cgtgctgacg atactgcttt tggcggcagt
    40141 cgggctgcac gcttccgtat atcgcacctt cacgcctgaa aacatccgca gccgcctcca
    40201 acaaagcatt gcacacacac accggaaaat ctcgtttgat gcggacattc agcgcaggct
    40261 cctgccccgg ccgaccgtca tcctgaaaaa cctgaccatt accgaacccg gcggcgacca
    40321 gactgccgtt tccgtccaag aaaccaaaat cggattgagc tggaaaaacc tgtggtcgga
    40381 tcagatacag attgaaaaat gggtggtttc gagtgcggaa cttgccctga cgcgcgacgg
    40441 gaaaggtgtt tggaacatcc aagacctgat cgacagccaa aaacgccaag cctcagtcaa
    40501 ccgcattatc gtcgaaaaca gcaccgtccg cctcaatttc ctgcaggaac agcttatcct
    40561 gaaggaaatc aacctcaacc tgcaatcccc cgattcgtcg gggcagccgt ttgaaagttc
    40621 gggcatactg gtttggggaa agctgtccgt cccgtggaaa agcagggggc tgttcctttc
    40681 agacggcatc ggcacgccca aaatctcacc gttccatttt gaagcttcca cttcgctgga
    40741 cggacacggc attaccattt ccaccaccgg cagcccttct gtccgcttca acgccggcgg
    40801 agcggatgcc gccggcctcg gcctgcgtgc agacacttcc ttccgcaacc tccacctgac
    40861 cgcccaaatc cctacgctgg cactcagaaa taacagcatt aaaattgaaa ccgtcaacgg
    40921 cgcatttacc gccggcggcg aatatgccca atgggacggt tcgttcaaac tcgacaaagc
    40981 caaccttcac tccggcatcg ccaacatcgg caacgccgaa atctccggca gcttcaaaac
    41041 accgcgccac cagaccaact tctccctcaa ttcgccgctc gtatggacgg aaaacaaagg
    41101 gctggacgcg ccgcgcctgt atgtatcgac ccttcaggat accgtcaacc gcctgccgca
    41161 accccgtttc atcagccggc tcgacggttc gctgtccgta ccgaatctgc aaaattggaa
    41221 tgccgaatta aacggcacat tcgaccgcca aaccgttgcc gcgaaattca gatacacaca
    41281 tgaagacgca ccgcatctgg aagccgccgt cgcactgcaa aaattgaacc tgacccccta
    41341 tcttgacgac gtgcggcaac aaaacggcaa aatatttccc gacaccctcg ccaagctgtc
    41401 cggcgacatc gaggcgcacc tgaaaatcgg aaaagtccaa cttcccggcc tgcaactgga
    41461 cgatatggaa acctacctcc acgccgacaa aggccatatc gcgctcagcc gtttcaagtc
    41521 agggctttac ggcggccata ccgaaggcgg catcagcatc gccaacaccc gtcccgccac
    41581 ttaccgcctg caacagaatg caagcaacat ccaaatccaa ccgctgctgc aagacctgtt
    41641 cggcttccac agcttcagcg gcaacggcga tgcggtcatc gacctgaccg cgggcggcga
    41701 aacccgaaaa gagcttatcc gctcgcttca gggcagcctg tcgctgaata tttccaacgg
    41761 cgcgtggcac ggtatcgaca tggacaatat cctgaaaaac ggcatttcgg gcaaaactgc
    41821 cgacaatgcc gcacccagca cacccttcca ccgattcacg ctcaacagcg aaatttcaga
    41881 cggcatcagc cgccacatcg ataccgaact cttctccgac agcctctatg ttaccagcaa
    41941 cggctatacc aatctggata cgcaggaatt gtctgaagat gtccttatcc gcaacgccgt
    42001 ccatccgaaa aacaaaccga ttcccctgaa aatcaccggt acggtggaca aaccgtccat
    42061 taccgtcgat tacggcaggc tgaccggcgg catcaattcg cgcaaagaga aacagaaaat
    42121 cctcgaagac accctgctgg aacaatggca gtggctcaaa cctaaagaac cgtaaacatc
    42181 ctgcgtacaa aaatgccgtc tgaaacaccc ccgcgcttca gacggcatga ccgtaaaacc
    42241 tacaacccca attcctccca aatcccatca atcttagccg taaccgcagg gtcttttttg
    42301 atgacgcgcc cccattcgcg gtcggtttct cccggccatt tgttggtcgc atccaaaccc
    42361 attttgccgc cgagtccgct aacggggctg gcgaagtcga gataatcgat gggcgtgttt
    42421 tctaccaaaa cagtgtcgcg cacggggtcc atgcgcgtgg ttaccgccca gatgacttct
    42481 ttccagtcgc gcacgttcac atcgtcatcc accacgatga tgaatttggt atacataaac
    42541 tggcgcagga acgaccagca gcccatcatc acgcgcttgg cgtgtccggc gtactgtttt
    42601 ttcatgccga tcaccgccat gcggtaggag cagccttcgg gcggcaggta aaaatcggtg
    42661 atttcgggga actgcttttg caaaagcggt acgaacactt cgttcaacgc cacgcccaaa
    42721 acagcgggtt catcgggcgg tttgcctgtg taggtcgaat ggtaaatcgg gttttcgcgc
    42781 atggtgatgc gctcgaccgt aaatacaggg aaatgatcct gctcgttgta atagccggtg
    42841 tggtcgccgt atgggccttc caacgcggtt tcgttcggat gaatcacgcc ttccaataca
    42901 atttcagcac gggcaggcac ttgcaaatcg ttgccgatac atttcaccag ctccgtccgc
    42961 gaaccgcgca gcagtccggc aaactggtat tcgctcaaag tatcgggaac aggcgttacc
    43021 gctccgagaa tagtggcagg gtcgcaaccg agcacgacgg cgacgggata cggcgtatcg
    43081 ggattgagtt tgcggaactc ctgataatcc aacgcgccgc cgcgatgcga cagccaacgc
    43141 ataatcagct tgtttttgcc gatgagttgt tggcggtaaa tgccgagatt ttggcgtttt
    43201 ttgtgcggcc cgcgcgttac ggtcaagccc catgttacca gcggcgcaac gtcttccggc
    43261 cagcaatgct gaatcggaag tttatataaa tcaacgtctt cgccttccca cacgatttcc
    43321 tgacacggcg cgtttttcac cacgttcggc gccatgctcc aaatgtcttt caagagcggc
    43381 agtttggaaa acgcgtcttt gatgcctttg ggcggttcgg gttctttcag atacgccagc
    43441 gtctgcccga tttcgcgcag cttggacacg ctgtccgcgc ccatgcccat cgccacacgt
    43501 tcgggcgtgc caaacaaatt cgccaacaca ggataatcgt agcgcgtacc gtcgggctta
    43561 atcgggtttt caaacaacaa cgccggccct tcggcgcgca acacgcggtc ggcgatttcg
    43621 gtcatttcca aatgcgggga aacaggatgc gcgacgcgct tgagtttgcc ctgttgctcg
    43681 agcatagcga tgaagtcgcg caggtctttg tatttcatat tcatcctttt tgtcctttta
    43741 tcctgagcaa tccgattcgg ataccgcccc tatccttgcc tgcgcttcgg catattctat
    43801 gccgtgataa aagtcgcgta ccagcggatg tccgccactt tgatgaagtt gcagcaaagg
    43861 gcgttggttg tcggactggg taacgacatt gcagtgcaga ccgaaggtgt cggtttcata
    43921 agggggcagc cagttgcaga tcatgccgaa ataaacgacg ttttccggat tgtcgtgaaa
    43981 gtggctttga tagtcgttaa aactcttttc actgacagat acccataccc catattccag
    44041 cgtttcctca tgcccgataa tcggaataag cagcaccgcg cggataaaac ggtcggtttg
    44101 gtcgggatag cggatgatgc agaaatcaga atcacattcc gcttgataag caatgcgttc
    44161 ttcttcactg agttgattat agggataggg ggcggtaaag ccgattgcgg gcatatcttc
    44221 atgtttttcg ccgcaggaag tgcaagtgta cataaggttt cagactttca aaacgagttt
    44281 gcggtaaagc cattcgccgg caaacggcat ccccatcaat acataggcaa tcacgccggt
    44341 ataaaccgcc caccaatcat atcgccccaa ccgtgccaac aaagcggcaa gcgtcccgtt
    44401 gattataaaa aatacgcacc aaacctgcgt tacccggcgg gtatagcgca cggctttttc
    44461 aggcaggtcg ggctgttgca gccgcgcaag tttttcaatc accgtctgcc cggcaaacag
    44521 gcttcccccg aacaccgcca acatcatcag attgacgagg acgggatacc aatacattga
    44581 gtcgcgccgc ccgaacacca atactgcggc aaaaaacagt gtaataaaca aagccgcata
    44641 acgctgttgg ggcaatttgg cagtcagggc gcgcagcagc cacaacccgc acatcgccgc
    44701 cgccagccag acaaaccagc ccgcctccct gccgtaatga tagtggatta acaaaaacca
    44761 gtacagcgtt gcctcgcctt gccgtactat ctgcggcttc gtcgccttgt cctgattttt
    44821 gttaatccac tataccacaa agcgggatag gcaatgctta atacggtcag aaaaatatgt
    44881 ccgaaaaaac cgggtttcat tttgaatccg cacaaatgtt ttcagacggc atccgataaa
    44941 aacatgccgt ctgaaaaata attagaaata cccgattagc ccgcctgaat cttcaatacc
    45001 gcctgtacca cgtcgttgac ggtgcggaca ttgcggaaat cttcagcctg cagcttgcgg
    45061 gctgccacag ccattcacga ttgcgatata cccggcggca ttcccgactg ccgttcagca
    45121 gaaagcgcac ccattccaaa ccgctccaat atgcctcggg cagcataccg gcttcagacg
    45181 gcatatcgtc cgaagcagat aaagtcaggc tgtaacgcgt ccctttggtc agaaccatcg
    45241 ccaaagcata agcaaacggc gcgcgcgttg ccgatacggc atatccttcc ggcagcggat
    45301 cgtccgccgc caaaaccaaa accgacccgc atccctcttc caacagtgat gccgcttccg
    45361 ccaatgccgt ttccacaccg tcggcacaca cggccaatgc cgtctgctcg ctcatatccc
    45421 gcctcaatat cgaccattac cccgccgttg cattgtgcac cgacaaaccg aacgaagtcg
    45481 gcgacacggt ttgcgatttc aacagttcca accacaaatc gaaactgcgc gccatttccc
    45541 cgtcgtgcga ggcataaact atagtggatt aaattttagg ggctgtacta gattagcaga
    45601 tatgttaccc tcgaaatatg aagataacac actgcaaatt aaagaaaaaa gtacagaaag
    45661 aactgctccg ttttttgtgc tggaagttac cgcccgttct gccgccgata ttttgggtat
    45721 ccatcccaat tcggcagcac tgttctaccg taaaatccgc acggttatca accatcattt
    45781 ggccttggct gccgatgagg tttttgaggg ccctgtcgag ccggacgaaa gcgatttcgg
    45841 cggacggcgt aaaggtagac gtggtcgcgg gcggcaggaa aagtggttgt cttcggcatt
    45901 ctgaaacgca acggacgggt ctataccgtt gtcgtaaaca atgccaagtc tgaaacgtta
    45961 ctccctgtca tcaaaaagaa aatcatgccg gacagtattg tttataccga tagtctgagc
    46021 agctgcgaca agttggacgt gagcggtttt atccattacc gcatcaacca ttccaaggaa
    46081 tttgcagacc gtcaaaacca cattaacggc attgagaact tttggaatca ggcaaaacgc
    46141 gtcttgcgaa aatatagtgg attggcacga acgtttcccc gaattggaag cgcaactggg
    46201 tgcgccgatt gaggattacg cgccgccgaa acattggacg cgcaagcagc tcagaagtat
    46261 ggggcgtgtg tcgtatttgt gcgtcgatgc ggcggagcag gctttgacgg atgccggttt
    46321 gctcggagac gaaagcatta ccgacggacg catgggcgtt gcctgcggct cttccagcgg
    46381 cagcaccaaa gacatcggcg atgtaggcga attgttgctg accggcatgt cgcgcaactt
    46441 cagtgccaac acctatgtgc gtatgatgcc gcacaccgcc gccgccaata tcggcatctt
    46501 tttcgggctg aaaggacgca tcatcccgac atcgagcgcg tgttcgtccg gcagccaagg
    46561 cataggttat gcctacgaag ccatcaaata cggtctgacc gatatgatgc tggcgggcgg
    46621 aggcgaagaa tttttcccgt ccgaagtgta tgttttcgac tcgctttatg ccgccagccg
    46681 ccgcaacggc gaaccggaaa aaaccccgcg cccatacgac gcgaaccgcg acgggctggt
    46741 catcggcgaa ggcgcgggga ttttcgtgct ggaagaattg gaacacgcca aacggcgcgg
    46801 tgcgataatt tacgccgaac tcgtcggcta cggagccaac agcgatgcct accatatttc
    46861 cacgccccgc cccgacgcgc aaggcgcaat ccttgccttt cagacggcat tgcaacacgc
    46921 aggtcttgcg cccgaagaca tcggctggat taatctgcac ggcaccggga cgcaccacaa
    46981 cgacagtatg gaaagccgtg ccgttgcagc ggttttcggc aacaatacgc cctgcacgtc
    47041 caccaagccg caaaccggac acacgctggg tgcggcgggc gcaatcgaag ccgcgttcgc
    47101 gtggggcatt gccgaccggc aaagcaatcc cgaaggaaaa cttccgcccc agctttggga
    47161 cgggcagaac gaccccgacc tgcccgccat caacctgacc ggcagcggca gccgctggga
    47221 aaccgaaaaa cgcattgccg ccagctcgtc gtttgccttc gggggaagca actgcgtctt
    47281 aatcatcgga tgaaataagt ttgtcaatcc caccgctatg ctatacaata cgcgcctact
    47341 cttgacgggt ctgtagctca ggggttagag caggggactc ataatccctt ggtcgtgggt
    47401 tcgaacccca ccggacccac caattcccaa gcccggacgt atgtttgggc ttttttgccg
    47461 ccctgtgaaa ccaaaatgct ttgagaaacc ttgataatga aaaaagtcag cgtattgatt
    47521 gttgccaaaa acgaagcaaa ccacattcag gaatgtattg aaagttgccg tttcgataaa
    47581 gaagtcatcg ttatcgacga ctacagcacc gacaatactg ccgaaattgc cgagggtttg
    47641 ggcgcaaaag tcttcagacg gcatttgaat ggggatttcg gagcgcaaaa aacatttgcc
    47701 atcgaacagg caggcggaga atgggttttc ctgattgatg cagacgaacg ctgcacgccg
    47761 gaactatctg atgaaatctc aaaaattgtc caaaccggcg attatgccgc ctattttgtc
    47821 gaacgccgca accttttccc caaccatccc gccacacacg gcgcgatgcg tcccgacagc
    47881 gtatgccgtc tgatgccgaa aaaagacagt tcggtgcaag gcaaagtaca cgaaaccgta
    47941 caaaccccct accccaaacg ccgtctgaag cattttatgt accattacac gtacgacaac
    48001 tgggaacaat atttcaacaa gttcaacaaa tatacttcca tttcagccga aaaataccga
    48061 gagcagggaa agcccgtgcg tttcgttagg gacattatcc tccgcccgat ttgggggttt
    48121 ttcaaaattt atatcctgaa caaagggttt ctcgatggaa aaatgggttg gattatgtcc
    48181 gtcaaccaca gctattacac gatgattaaa tatgtcaaac tatattatct gtacaaatct
    48241 ggcggaaaat tttaaatgga aaaagaattc aggatattaa atatcgtatc ggccaagatt
    48301 tggggtgggg gcgaacaata tgtctatgat gtttcaaaag cattggggct tcggggctgc
    48361 acaatgttta ccgccgtcaa taaaaataat gagttgatgc acagacgctt ttccgaagtt
    48421 tcttccgttt tcacgacacg ccttcacacg ctcaacgggc tgttttcgct ctgcgcgctt
    48481 acccgcttta tccggaaaaa ccgcatttcc cacctgatga tacacaccgg caaaattgcc
    48541 gccttatcca tacttttgaa aaaactgacc ggggtgcgcc tgatatttgt caaacataat
    48601 gtcgtcgcca acaaaaccga tttttaccac cgcctgatac agaaaaacac agaccgcttt
    48661 atttgcgttt cccgtctggt ttacgatgtg caaaccgccg acaatccctt taaagcaaaa
    48721 taccggattg ttcataacgg tatcgatacc ggccgtttcc ctccctctca agaaaaaccc
    48781 gacagccgtt tttttaccgt cgcctacgcc ggcaggatca gtccagaaaa aggattggaa
    48841 aacctgattg aagcctgtgt gatactgcat cggaaatatc ctcaaatcag gctcaaattg
    48901 gcaggggacg gacatccgga ttatatgtgc cgcctgaagc gggacgtatc tgcttcagga
    48961 gcagaaccat ttgtttcttt tgaagggttt accgaaaaac ttgcttcgtt ttaccgccaa
    49021 agcgatgtcg tggttttgcc cagcctcgtc ccggaggcat tcggtttgtc attatgcgag
    49081 gcgatgtact gccgaacggc ggtgatttcc aatactttgg gcgcgcaaaa ggaaattatc
    49141 gaacatcatc aatcggggat tctactggac aggcttacgc ctgaatcttt ggcggacgaa
    49201 atcgaacgcc tcgtcttaaa ccccgaagca aaaaacgcac tggcaacggc aggacatcaa
    49261 tgcgtcgcca accgttttac catcaaccat accgccgaca aattattgga tgcaatataa
    49321 actgctttca gacggcctat gccgtctgaa agcctttgat gcaacaaacc gccaaattat
    49381 attcgttcat tggaaagaaa caccccgaat tcatccttca aaataagaaa atcccaatat
    49441 cccccgatat tacgcagcct attggcaaag ttttgcagcg tcttccccgg cttgtgccgc
    49501 cgcgtcaagt gctttgttac aatgcactac cttcacattt cttaataaat tttatgagta
    49561 accatacttc ttggtcgtcc aaaatcggtt tcgtacttgc tgcagcaggt tcggccatcg
    49621 gtttgggcgc gatttggaaa tttccttata cggcaggcac caacggcggc gcggtgtttt
    49681 tcctgctgtt tttgatattt actatcttgg tcgccctacc cgttcagctt gccgaatttt
    49741 atatcgggcg cacgggcggt aaaaatgccg tcgattcctt cagggttctg cgtccgggca
    49801 cgcaatggct ttgggtcggg cgtatgggcg ttgccgcctg ctttattttg ctgtcgtttt
    49861 acagcgtggt cggcggatgg gtattaaatt atgtcgtcca cagttttacg ggggcggttc
    49921 ataccggcgc ggactttgaa gccttgttcg gcgcgacgat ttccaatccg gcaggttcgc
    49981 tgtcctatca ggctctgttt atgctgatta cggtttgggt ggtcaaaggt gggatttcag
    50041 acggcattga aaaggcaaac cgttatctga tgccggggct gtttatcctc tttattgcgc
    50101 tggcaatccg ttcgctgacg ctgccgggtg caatggaggg cgtgtcattc ctgctcaaac
    50161 cgaattggtc gtactttaaa gccgatacga tgattacggc tttgggacag gcgttttttg
    50221 ccctgagcat cggtgtttcc gccatgatta cctacgcttc atatttggga aaagatcagg
    50281 atatgttccg ctcggggcat acgattatgt ggatgaacct cttggtttcg ctgcttgccg
    50341 gcctggtgat ttttccggcg gtgttcgcct tcggttttga accgagccag gggccgggat
    50401 tgatttttat cgtattgccc gcagtgttta tgaagatgcc gttcggtacg gttttgtttg
    50461 cggtatttat gctgctggtc gttttcgcca cgctgacttc cgcattttcg atgttggaaa
    50521 cggtcatcgc cgcaaccatc cgccaagacg agcgcaaacg caaaaaacac acttggctta
    50581 tcggcacggc cattttcatt atcggcatcc catccgcgct gtctttcggc gtatggggtg
    50641 agtttaaggt tttcggcaaa accatttttg atttgtggga ctatgttatt tccgccgtca
    50701 ttatgccgat tggtgctttg agtgtttcca tctttaccgc ctggattcag gacaagcagt
    50761 ctgtgttaaa agatgccggc gcgggcagca ccgtaccacg ggcagtgctg ctgctgtggc
    50821 tgaatacctt gcgctacctt gccccgattg ccattattat tgttttcatc aattctttgg
    50881 acatccttta aaagccatcc aaacagcaaa aatgccgtct gaaagccttt cagacggcat
    50941 ttttgcttca ggttcagcct atttcgttca aagtatagtg gattaacaaa aatcaggaca
    51001 aggcgacgaa gctgcagacc gtacagatag tacggaaccg attcacttgg tgcttcagca
    51061 ccttagagaa tcgttctctt tgagctaagg cgaggcaaca ccgtactggt ttttgttaat
    51121 ccactatagc cttgcgcgat gccgttcaag gacaaaccca tacccttttc ggcaaaacgg
    51181 atttcacggt cgtcaaacga gactttgccg aagccgaccc gtttcagggc ttccttcacg
    51241 ctgttttgag gcggcggcgt ttccgcatcg ggacgggcgg caaaataatc ggcatacagt
    51301 ttccacaacg cctgcactgt cggatcgaac gcgccgtccg tcagcgcgtg aatatcgcgg
    51361 cacaggctca acagttccaa aaaatccgcc gacggcgaag tcagataacc gtccctgttc
    51421 aggcggctga tcaggctgtc ttcacggtaa aggctgaaca attttttcca aacgcgccac
    51481 ttccgccaaa accttgttga ccaaatccgc cgcacgcctg tcgtccacac cgaacagacg
    51541 gagctccgca ccggaaccca gtgcgacacc tttccagaaa aacacatttt cactgcgttt
    51601 ttcatccccg ttgcgttttt catcatcggc ggcaaaagga ttcggcaaaa aagaaaccgc
    51661 cgcgcccgcc gccgcaacgg cggcagccgt caaaaaacgc ctgcgcccga aatgcctgcc
    51721 cataccgcct cctaaaccga cactgccgcc ttgatgtgcg gatgcggatc gtagccttcc
    51781 aactcgaaat cttcaaactt gaaggaaaac aaatctttga cttcaggatt gattttcatc
    51841 actggcaagg cgcgcggttc gcgttccaac tgcaatgcgg cctgctcgaa atggttgcgg
    51901 tacaaatgcg cgtcgccaaa cgtatggaca aactcgcccg cctccagccc gcacacttgc
    51961 gccatcatca tcgtcaacag cgcgtagctg gcaatattaa acggcacacc aaggaaaata
    52021 tctgcactac gctggtaaag ctggcaggac agtttgccgt cggcaacgta aaactgaaac
    52081 agcgtgtggc agggcggcaa ggccatttca tcgaccaaag ccggattcca cgccgataca
    52141 atcaggcggc gcgagtcggg attcttcttg atttgttcca gcacattggc gatttggtcg
    52201 atatgcctgc cgtcgggcgc gggccagtta cgccactggt agccgtaaac cgggcctaag
    52261 tcgccgtttt cgtccgccca ctcgtcccaa atggaaacat tgttgtcctt taggtatttg
    52321 atattggtat cgcctttgag aaaccaaagc agctcgtgga taatcgaacg cagatgcagc
    52381 tttttggtcg tcagcagcgg aaaacctttg cccaagtcaa aacgcatctg ataaccgaat
    52441 acggagcgcg tacccgtacc ggtgcggtct gatttgtccg taccgttgtc gaggacgtgg
    52501 cgcatcaagt ccaaataggc tttcatagca gtctttcatc aaattaaacg gcgcatattg
    52561 taacatttcc ggataatgcc caaaacacgg atacaggcag gcaggattgt tggcaatttc
    52621 agtccttttc cacagtaaaa cccggtggga aaacaaaatt accttgattg gaatcaaaaa
    52681 atctagttta attacttaga ataaaatttc aataatattt ttatttacga aattaattta
    52741 tgattattga ttaattatcg gcaacaaaca tcaaacatcg aaaacatgga aaaaataatg
    52801 tcaacaattt ttgccaaatc gggcttggca tcagaaaaaa ataggtttat attcccacct
    52861 acaaatttgt tttcccatta gtacactatc aaccaaaagg agtatccgaa tgactgacct
    52921 gaacaccctg tttgccaacc tcaaacaacg caatcccaat caggagccgt tccatcaggc
    52981 ggttgaagaa gtcttcatga gtctcgatcc gtttttggca aaaaatccga aatacaccca
    53041 gcaaagcctg ctggaacgca tcgtcgaacc cgaacgcgtc gtgatgttcc gcgtaacctg
    53101 gcaggacgat aaagggcaag tccaagtcaa ccggggctac cgcgtgcaaa tgagttccgc
    53161 catcggtcct tacaaaggcg gcctgcgctt ccatccgacc gtcgatttgg gcgtattgaa
    53221 attcctcgct tttgaacaag tgttcaaaaa cgccttgacc accctgccta tgggcggcgg
    53281 caaaggcggt tccgacttcg accccaaagg caaatccgat gccgaagtaa tgcgcttctg
    53341 ccaagccttt atgaccgaac tctaccgcca catcggcgcg gacaccgatg ttccggccgg
    53401 cgacatcggc gtaggcgggc gcgaaatcgg ctacctgttc ggacaataca aaaaaatccg
    53461 caacgagttt tcttccgtcc taaccggcaa aggtttggaa tggggcggca gcctcatccg
    53521 tcccgaagcg accggctacg gctgcgtcta tttcgcccaa gcgatgctgc aaacccgcaa
    53581 cgatagtttt gaaggcaaac gcgtcctgat ttccggttcc ggcaacgtgg cgcaatacgc
    53641 cgccgaaaaa gccatccaac tgggtgcgaa agtactgacc gtttccgact ccaacggctt
    53701 cgtcctcttc cccgacagcg gtatgaccga agcgcaactc gccgccttga tcgaattgaa
    53761 agaagtccgc cgcgaacgcg ttgccaccta cgccaaagag caaggtctgc aatactttga
    53821 aaaacaaaaa ccgtggggcg tcgccgccga aatcgccctg ccctgcgcga cccagaacga
    53881 attggacgaa gaagccgcca aaaccctgtt ggcaaacggc tgctacgtcg ttgccgaagg
    53941 tgcgaatatg ccgtcgactt tgggcgcggt cgagcaattt atcaaagccg gcatcctcta
    54001 cgccccggga aaagcctcca atgccggcgg cgtagcaact tcaggtttgg aaatgagcca
    54061 aaacgccatc cgcctgtctt ggactcgtga agaagtcgac caacgcctgt tcggcatcat
    54121 gcaaagcatc cacgaatcct gtctgaaata cggcaaagtc ggcgacacag taaactacgt
    54181 caatggtgcg aacattgccg gtttcgtcaa agttgccgat gcgatgctgg cgcaaggctt
    54241 ctaagcaaac gccgccgctc cgcaaacaaa atgccgtccg aaccgcaaat gctgttcaga
    54301 cggcatttca ttatccgccc gttcgaatcg ggtgatacta tttgtatcca gaattgttgg
    54361 atagtttggg agtggagagg aagaaatgat tatggaccaa tactatatgg agttaaagaa
    54421 taagttatct aacagaccaa tcctactgga taatactaat gattttcttt ttgttttagt
    54481 aaatacagtg aaagctatga tagaaaatac tgataaaagt cagctttcag agttagataa
    54541 aattttagac ggtgtcacaa gtcaagaatt gaaacttgcc tatgattttt gtcaagggaa
    54601 attcggtcaa gcaggttttt cttatcgtag gcacccgaat tatttttatt tgtccagttt
    54661 aattgcgact tttccagaat ttgagttgtc taaagcagac cgagattacc tcaaaggaat
    54721 cataaatttt gataactacc ttttatatga gttagattag tctttaatca cctcaaaacc
    54781 ctatttattc aaaaggagaa aaaatgattg agctgaaatt agtagatgag agcagttttc
    54841 aggcagtgct ggatttgaaa atatctgaag ctgatgaacg agcacgtttt gtggctccaa
    54901 atgtacgctc tttagctgac gcatggctct accgggaaaa tgaagatgtg tttccgaggg
    54961 caatctattg ggataagcaa gtggttggct ttctcctgct ggaaatagac aaggatgaag
    55021 cagaatactt tatctggcgg ataatgattg gtcagcagtg aattaacaaa aatcaggaca
    55081 aggcggcgag ccgcagacag tacaaatagt acggaaccga ttcacttggt gcttcagcac
    55141 cttagagaat cgttctcttt gagctaaggc gaggcaacga cgtactggtt taaatttagt
    55201 tcactatacc gatacatccg aatatgccag accgtctaaa cggtattcat acctcccaat
    55261 cctgcacgcg cttcaaatcg ttttgcgcca aagtatccgc gtggcggtta cggctctgat
    55321 attccctgtc tttcatgatg ctgcttgcca cataattcaa atgtgccttt gccgcctccg
    55381 aagcctcgcc cggccgacgg ttcgatattg cctcatacaa tacacggtgc tgcgccatca
    55441 gcttcggacg cggatcttct tcctgattca gataaataag gctgctgcgc gtctgccggt
    55501 acagcatttt caacaaaccg cccgacaaat ggctgaacaa caaattgtgc gccgcatcgg
    55561 caatcgtctg atgaaagctg acatcagctt cgctctgatg ttccaaattg ccgctttcgc
    55621 acgcctcctc aaacttttca agccaaaacc caatccgctt caaatcggca tccgtgcggc
    55681 gttctgccgc caatgccgcc atacagccct cgatgtggca actgaaatca aaaacatcct
    55741 gttcccaatt ggaatgcttg cccaaaagct cctgccaact ttgcaaaaaa tcctgctgcg
    55801 gcttgaccga aacataataa ccgtctccct gcctcgcttc caaaacctga cgggcgacca
    55861 aaacattcaa tgccgacctg accgacgggc gcgaaacgcc gaactcttcc gccaaaacgc
    55921 gttcgggcgg aatcttgccc ccttccgcgt aaaccccttc cgcaatgcgc tcctccaata
    55981 ccgacaatac ctgatcgctg attttctgag gccttaccag tttcatcact cctcctttat
    56041 aaagattccc tgcagaaccc ttccgaaata tagtggatta acaaaaatca ggacaagg
//
LOCUS       DAWFLT010000003        50211 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWFLT010000003 DAWFLT010000000
VERSION     DAWFLT010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 50211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50211
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.003"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..68)
                     /locus_tag="WGR74_000160"
     CDS             complement(<1..68)
                     /locus_tag="WGR74_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0904667.1"
                     /translation="MLPSKYEDNALQIKEKSTERTAP"
     gene            complement(271..987)
                     /gene="trmB"
                     /locus_tag="WGR74_000161"
     CDS             complement(271..987)
                     /gene="trmB"
                     /locus_tag="WGR74_000161"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ0904668.1"
                     /translation="MTDTAENQTQNNWQAEHPRSIRSFVLRQSHMTAAQQRAIDTLWG
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIEENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(1109..2195)
                     /locus_tag="WGR74_000162"
                     /pseudo
     CDS             complement(1109..2195)
                     /locus_tag="WGR74_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(2468..4354)
                     /gene="uvrC"
                     /locus_tag="WGR74_000163"
     CDS             complement(2468..4354)
                     /gene="uvrC"
                     /locus_tag="WGR74_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ0904669.1"
                     /translation="MPSEAVFQIIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNLI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IINNFPVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSNQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGIAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     EHLH"
     gene            complement(4409..6586)
                     /locus_tag="WGR74_000164"
     CDS             complement(4409..6586)
                     /locus_tag="WGR74_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ0904670.1"
                     /translation="MQQKVRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLAINIPFLIG
                     MVGMMLKGLNWTRHDWMLSPLLQFALASVVQLWLAVPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSPHAAYGMAHVYFEVGIMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRDGEWRQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGIGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGSPQVAAVYCVPDSGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLDIPAAQNAQTVVGAGITAEVEGVGLVKA
                     GKAEFAELALPKFLDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNI
                     DVYIMSGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALSVSRATLRNIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            6725..7675
                     /gene="trxB"
                     /locus_tag="WGR74_000165"
     CDS             6725..7675
                     /gene="trxB"
                     /locus_tag="WGR74_000165"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ0904671.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGTELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            7831..8199
                     /gene="rpsF"
                     /locus_tag="WGR74_000166"
     CDS             7831..8199
                     /gene="rpsF"
                     /locus_tag="WGR74_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ0904672.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            8200..8502
                     /gene="priB"
                     /locus_tag="WGR74_000167"
     CDS             8200..8502
                     /gene="priB"
                     /locus_tag="WGR74_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ0904673.1"
                     /translation="MGFNNLVSLAVLIEKVFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            8509..8739
                     /locus_tag="WGR74_000168"
     CDS             8509..8739
                     /locus_tag="WGR74_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ0904674.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            8756..9208
                     /locus_tag="WGR74_000169"
     CDS             8756..9208
                     /locus_tag="WGR74_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ0904675.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTITVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            9222..9329
                     /locus_tag="WGR74_000170"
     CDS             9222..9329
                     /locus_tag="WGR74_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HEZ0904676.1"
                     /translation="MPSETLPIVGMWFQTVFVLFFVYSAKIGMVVFIVD"
     gene            complement(9996..10802)
                     /locus_tag="WGR74_000171"
     CDS             complement(9996..10802)
                     /locus_tag="WGR74_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0904677.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFTMMPGMVFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(10803..11549)
                     /gene="pssA"
                     /locus_tag="WGR74_000172"
     CDS             complement(10803..11549)
                     /gene="pssA"
                     /locus_tag="WGR74_000172"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ0904678.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            12132..12615
                     /locus_tag="WGR74_000173"
                     /pseudo
     CDS             12132..12615
                     /locus_tag="WGR74_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
     gene            complement(12667..13878)
                     /locus_tag="WGR74_000174"
     CDS             complement(12667..13878)
                     /locus_tag="WGR74_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ0904679.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGIKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            14101..16539
                     /locus_tag="WGR74_000175"
     CDS             14101..16539
                     /locus_tag="WGR74_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ0904680.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAGAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDNPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHHVVAFVKEQAPANYVEGLLTGEAAQETAN
                     IVSPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPSD
                     LNGSRKILAHKDHL"
     gene            16747..18057
                     /locus_tag="WGR74_000176"
     CDS             16747..18057
                     /locus_tag="WGR74_000176"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ0904681.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFHDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            18153..18767
                     /gene="clpP"
                     /locus_tag="WGR74_000177"
     CDS             18153..18767
                     /gene="clpP"
                     /locus_tag="WGR74_000177"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ0904682.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQVLENRASLQL"
     gene            complement(18820..20085)
                     /gene="ispG"
                     /locus_tag="WGR74_000178"
                     /gene_synonym="gcpE"
     CDS             complement(18820..20085)
                     /gene="ispG"
                     /locus_tag="WGR74_000178"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ0904683.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(20101..20862)
                     /gene="pilW"
                     /locus_tag="WGR74_000179"
     CDS             complement(20101..20862)
                     /gene="pilW"
                     /locus_tag="WGR74_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ0904684.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLIGQ"
     gene            complement(20865..21959)
                     /gene="rlmN"
                     /locus_tag="WGR74_000180"
     CDS             complement(20865..21959)
                     /gene="rlmN"
                     /locus_tag="WGR74_000180"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ0904685.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFNE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(22103..22528)
                     /gene="ndk"
                     /locus_tag="WGR74_000181"
     CDS             complement(22103..22528)
                     /gene="ndk"
                     /locus_tag="WGR74_000181"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ0904686.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(22587..23738)
                     /locus_tag="WGR74_000182"
     CDS             complement(22587..23738)
                     /locus_tag="WGR74_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ0904687.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
     gene            complement(23922..24025)
                     /locus_tag="WGR74_000183"
                     /pseudo
     CDS             complement(23922..24025)
                     /locus_tag="WGR74_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(24132..24959)
                     /gene="fghA"
                     /locus_tag="WGR74_000184"
     CDS             complement(24132..24959)
                     /gene="fghA"
                     /locus_tag="WGR74_000184"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ0904688.1"
                     /translation="MKLIEQHQIFGGSQQVWAHNAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVASDTSPRGEQVPNDDAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            complement(24968..26077)
                     /locus_tag="WGR74_000185"
     CDS             complement(24968..26077)
                     /locus_tag="WGR74_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ0904689.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGVGQEISTRPFQLVTGRVWKGTAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINEAFELMHEGKSIRAVIHY"
     gene            26225..26632
                     /locus_tag="WGR74_000186"
     CDS             26225..26632
                     /locus_tag="WGR74_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ0904690.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            complement(26983..27297)
                     /locus_tag="WGR74_000187"
     CDS             complement(26983..27297)
                     /locus_tag="WGR74_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ0904691.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(27308..28993)
                     /gene="rpsA"
                     /locus_tag="WGR74_000188"
     CDS             complement(27308..28993)
                     /gene="rpsA"
                     /locus_tag="WGR74_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ0904692.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQDKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDESRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            complement(29149..29805)
                     /locus_tag="WGR74_000189"
     CDS             complement(29149..29805)
                     /locus_tag="WGR74_000189"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ0904693.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSDSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRDSLLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYREV
                     "
     gene            complement(30049..30858)
                     /locus_tag="WGR74_000190"
     CDS             complement(30049..30858)
                     /locus_tag="WGR74_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0904694.1"
                     /translation="MPLLFLFLIAMVVRNVTLPGAMEGVAFYLKPDFSKITAELFVFV
                     LGQVFFALSLGFGVMITLSSYLDKNENLVQTAVITAITNTIIAILAGFMIFPSLFSFG
                     VAPDSGPTLVFQSLPIVFSHMWAGSVFAVIFFSLLLIAALTTSLTIYEVLITTIQEKT
                     KIRRTAAITIVLAAIYVFGNIPSILSYGPWKDVSVFGKNIFDAFDYISGNILFMLTAL
                     GSALFVGFVMKDEAKEELLYKGNHTTVNIWFAYVKYLVPLVILLIFVSNLF"
     gene            complement(30918..31361)
                     /locus_tag="WGR74_000191"
     CDS             complement(30918..31361)
                     /locus_tag="WGR74_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0904695.1"
                     /translation="MTVAGATVGFGATWRFPYLVGENGGGAYVFLFCIAMLVIGIPMI
                     LVENVIGRRKGVNALDAFGGPMNGKPIAKIWKLVGWMGLLGAFGIMAYYMALGGWVIS
                     YIVNIIGGNLNISSPIDGVVTKGFFAEHIETALGKLRFIRCFLSP"
     gene            complement(31890..32582)
                     /locus_tag="WGR74_000192"
     CDS             complement(31890..32582)
                     /locus_tag="WGR74_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEZ0904696.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPAGETLCETLKNGVLLHD
                     ENETVYAADAVLESPSALLLTITEGKYHQVKRMVAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIDCPKF"
     gene            complement(32650..34626)
                     /locus_tag="WGR74_000193"
     CDS             complement(32650..34626)
                     /locus_tag="WGR74_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0904697.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIVPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRVRLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            34804..35469
                     /locus_tag="WGR74_000194"
     CDS             34804..35469
                     /locus_tag="WGR74_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ0904698.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGIPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRQLADVGFDIEFGKFMVYLPPVS
                     SIGQIRFWRFMEKAGDRWWPQCAAVYALVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     VAD"
     gene            35521..36348
                     /gene="mutM"
                     /locus_tag="WGR74_000195"
     CDS             35521..36348
                     /gene="mutM"
                     /locus_tag="WGR74_000195"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ0904699.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYARLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            36436..37203
                     /locus_tag="WGR74_000196"
     CDS             36436..37203
                     /locus_tag="WGR74_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0904700.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(37515..37826)
                     /locus_tag="WGR74_000197"
     CDS             complement(37515..37826)
                     /locus_tag="WGR74_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ0904701.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYKEQKTQCRAAYEAFDDFDGSRFR
                     R"
     gene            38355..40634
                     /gene="nrdA"
                     /locus_tag="WGR74_000198"
     CDS             38355..40634
                     /gene="nrdA"
                     /locus_tag="WGR74_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ0904702.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAAKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCNEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQAISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            40692..41705
                     /gene="dcm"
                     /locus_tag="WGR74_000199"
     CDS             40692..41705
                     /gene="dcm"
                     /locus_tag="WGR74_000199"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0904703.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            41698..42885
                     /locus_tag="WGR74_000200"
     CDS             41698..42885
                     /locus_tag="WGR74_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction enzyme"
                     /protein_id="HEZ0904704.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            42946..44079
                     /gene="nrdB"
                     /locus_tag="WGR74_000201"
     CDS             42946..44079
                     /gene="nrdB"
                     /locus_tag="WGR74_000201"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ0904705.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPITVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            44277..44567
                     /locus_tag="WGR74_000202"
     CDS             44277..44567
                     /locus_tag="WGR74_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ0904706.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
     gene            complement(44608..44886)
                     /gene="ftsB"
                     /locus_tag="WGR74_000203"
     CDS             complement(44608..44886)
                     /gene="ftsB"
                     /locus_tag="WGR74_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ0904707.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(44900..46186)
                     /gene="eno"
                     /locus_tag="WGR74_000204"
     CDS             complement(44900..46186)
                     /gene="eno"
                     /locus_tag="WGR74_000204"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ0904708.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPSKAAFYQLGK"
     gene            complement(46233..46673)
                     /locus_tag="WGR74_000205"
     CDS             complement(46233..46673)
                     /locus_tag="WGR74_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ0904709.1"
                     /translation="MMQTFRKISRYAATLWLGMQIMAGYIVAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANRFLITPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSIVFMATALLSAVLSWRLSGKDAV"
     gene            complement(46688..47530)
                     /gene="kdsA"
                     /locus_tag="WGR74_000206"
     CDS             complement(46688..47530)
                     /gene="kdsA"
                     /locus_tag="WGR74_000206"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ0904710.1"
                     /translation="MDIKINDITIGNNSPFVLFGGINVLESLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVVAMAKTGNVVNIKKPQFLSPSQMKNIVEKFHEAGNGKL
                     ILCERGSSFGYDNLVVDMLGFCVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            complement(47552..47935)
                     /locus_tag="WGR74_000207"
     CDS             complement(47552..47935)
                     /locus_tag="WGR74_000207"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ0904711.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            complement(48084..>50211)
                     /gene="mfd"
                     /locus_tag="WGR74_000208"
     CDS             complement(48084..>50211)
                     /gene="mfd"
                     /locus_tag="WGR74_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ0904712.1"
                     /translation="AIAVITESDLYQYVARSRKYHRKKHAAVSDGLLRDLAEINIGDP
                     VVHEEHGIGRYMGLVTMDLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHENI
                     ALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAQRAAQSGHKFEINELDYQAFADGFG
                     YEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAVLA
                     PTTLLVEQHAQNFADRFADFPVKVAGLSRFNNSKATKAALEGMADGTVDIIIGTHKLV
                     QDDIKFKNLGLVIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGLRD
                     FSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRERLE
                     TLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIINRA
                     DKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLAMQ
                     DLEIRGAGEILGEGQSGEMIQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEIKL
                     HSPALLPESYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLPEQPVKTLIESH
                     HLRLAAKELGIDAIDAAGESVTVTFGKNHNVDPTEIILLIQNDKKYRLAGADKLRFTA
                     EMENIEVRINTVKNVLKTLQNRCLPK"
BASE COUNT        11944 a        11839 c        13585 g        12843 t
ORIGIN      
        1 ggagcagttc tttctgtact tttttcttta atttgcagtg cgttatcttc atatttcgag
       61 ggtaacatat ctgctaatct agtacagccc caactttaaa accgatgtgc aggttaaaac
      121 ctacaaggat gcgtgcggta tacacgcgtt tttgccgttc aaaccattgc tttgaacttt
      181 gtatcgaaac atcgttcgct gctttatctt gaaaaaatgc cgtctgtaag caaacatgct
      241 tcagacggca tttttttatc cggcggtttg ttatccgatc cgtttgaata ccaagtccca
      301 aacgccgtgt ccgaggcgtt tgccgcgcgc ttcgaatttg gtttcggggc ggtagtccgg
      361 ggtgggggcg tagtctgccg ccgtattttg caggctgtcg aagctgctga ggacttcaag
      421 catctgctgt gcatattctt cccagtctgt cgccaggtgg atatagccgc cggttttgag
      481 tttgggcagc agtttggcga tgaagggggc ttgtatcaga cggcgtttgt ggtggcgttt
      541 tttgtgccac gggtcgggga agaatatgtg gatgccgtcg agcgagccgt cttgcagcat
      601 attttcgaca acttctacgg cgtcgtgccg catcacgcgg atgttttcca aatggttttc
      661 ttctatgagt ttgagcagat tgcctacgcc gggaccgtgt acgtcgatgg caagaaagtc
      721 ggtttcgggc agcctgcggg cgatttctgc ggttgccgtc cccataccga agcctatttc
      781 gaggattttg ggtcggctgc ttccgaaacg ggcatcaaga tcggccggtg ttgcttggta
      841 gtcgatgccg aagctgcccc ataaggtatc gatggcgcgt tgctgcgcgg cggtcatgtg
      901 gctttggcgg aggacgaaac tgcggatgct gcgggggtgt tcggcttgcc agttgttttg
      961 tgtttggttt tcggcggtgt ctgtcataat gctttcgtgc gcttgtgcaa tcaggatgcc
     1021 gtctgaatgt gaaaggcctg ttcggacggc gggaaaggaa aggggcgatt atacctgttt
     1081 cgtttcggca gggtaggtgt aacgctgttc agacggcatt ttgccattgt tgcagcagtt
     1141 cttgcaggac gcttttttgc ccgtatccgt tgtttatggc ttcttgcagg tagcggcagg
     1201 ctttttcttt gtccggctcg acgccgtgtc cgttgtaatg gatgcaggcg aggttataaa
     1261 gtgccatgct gtctttttgc gcggcggctt tttcaaacca tttgcgtgct tcattgtagt
     1321 cggcggtcat accctgtccg aagtaataca tgctgcccag attggtttgg gctttgctgt
     1381 ggccgagttc tgcggcttct gtgtagagtt tgagtgcggc tgcatattcg ggcgcgcatt
     1441 gtaatccgta atgcagggct tgggcttttt gataaagccg ttctgccctt tgccgcctgt
     1501 tgaaaccagt gaacggcttc atcaggagcc gtaggtagga tgagctcggc aagcggggtg
     1561 taggcgggaa tgtatccttg ttgtgcggcg gcgcgcaaat gattgacggc ctgtgccgtg
     1621 tcgactttcg tcccttgtcc gaaacggtat tgcagcccga gctgccagtg tgccgccgcc
     1681 aagccttgtg ccgccgcttt gcggtagagc aggtgcgccg tttcaaaatc cggcggcgtg
     1741 ccgtaccggc agtattgggc aagcaggtat tgcccgtggg ggttgttgtt ttttgcgctt
     1801 gtttttgccc atttcagggc ttcttcaaaa tgtcgggacg catacagaag gtacatcagg
     1861 cggacttgtg cttcaggcag tccttcctct gccaacggtc tgtaaagcgg tatcagggtt
     1921 tccgggggcg cgccttccaa ttcttggata tatgccagat tgtaacgcgc atagggatgc
     1981 ccctgttcgg cggcgatgcg gagcatttcc ataccttgtt cgcggtcttg gtaggggctg
     2041 tccgcgtcca tcaggtgttt tgccaactcg aatgcggcgg cgggatgacc tgccgagaac
     2101 gcttctgtca gcagtcggcg gacttcggta aagttatatt gtttttgaga caggcattct
     2161 aaggcctgcc gatagggtaa gtcgggattg ttcatgtcag atcgttttcc gttttgtcga
     2221 atcatcgtta ttgtgttatg aagttaggtt tatagtggat taacaaaaat caggacaagg
     2281 cgacgaagcc gcagacagta caaatagtac gaaaccgatt cacttggtgc ttgagcacct
     2341 tagagaatcg ttctctttga gctaaggcga ggcaacgccg tactggttta aatttaatcc
     2401 actatatatc ggtataccga cctgctgtat catatttttt acagacggtt tttgcttggg
     2461 cagcatacta atgcagatgc tcgtaaatgg tttccgccaa tgccttgctg atgccttcca
     2521 ctttttccaa gtcctcgcgg ctggcggcaa tcacgccgcg cagaccgccg aagcgggtga
     2581 gcagggcttg gcggcgtttg ctgcctacgc cggggatgtc gcttaaggag gaggtaacgc
     2641 gggctttgtc gcgttttttg cggtggccgg taatggcgaa acggtgcgat tcatcgcgta
     2701 cggtttgcaa tagatgcaag gccgggctgt tgggcggcag gcggaagact tcgccggtaa
     2761 aaggcagtat gagttcttcc ataccggctt tgcgttcggg gcctttggcg ataccgacca
     2821 aaggaatgtg cagcccgagt tcttcccata ccgatacggc gatgccgatt tgccctttgc
     2881 cgccgtcaat taacacggca tccggccatt tgacggtctc gccgttggct tcggcttcct
     2941 gcattttgcc gtaacggcgc gtcaacactt cgcgcatggc ggcgtagtcg tcgccgggtt
     3001 tggcggtcgt gatgttgtag cggcggtatt gcgagggctg gatgttttgc tcatcgtaca
     3061 cgacgcagga ggcgatggtg gcttcgcctt gggtgtggct gatgtcgaag cattcgaggc
     3121 ggttgaggcc gtctgaatcc ataccgagga ttttggcgag ttcgtcgatg cggtgttgct
     3181 ggttgctttg ttgcaggcgg cgttgtgcaa tcgccatttg tgcgttttgt tccgccattt
     3241 tcaaccaaac tttgcgttcg cctatggttt tggttacgaa ctgcatttgt ttgccgtgtt
     3301 cgccttccaa agcctctttc agcgcatcgg ggacggggaa gttgttgatg ataatgtcgg
     3361 gtttgctttt gcccagatag tgttgggcga cgaaggcttc ggcatagtct tgcccgtttg
     3421 gctcgggatc gtttttggtg tcggggaaga aacttttgtc tccgacgtgc cgcccaccgc
     3481 gtatgctgac ccagtgtacg cagacgatgc cgtctgaaac cgccagcgcg agcaaatcga
     3541 tatcgttggg gttgttcggg tttttgctgt cgatgaactg gttgctctgc ataatgccga
     3601 gtgcttggat ttggtcgcgg tagcgggcgg cttcttcaaa ttgcagattc gcggcggcag
     3661 tttgcatttt gtgttgcagg gtgcgggtca gttcgtcggt tttgccgttg agaaaagtgg
     3721 cggcttgacg cacgctgtcg cggtagtctt cttcgctgat gtgtccgacg cagggcgcgg
     3781 tgcagcgttt gatttgatac agcaggcaag ggcggtcacg atgctcgaat acgctgtctt
     3841 cgcaggtacg cagcataaag actttttgca acacttgaat gctgtcgcgc acggcgttgc
     3901 tgttgggata cgggccgaag tattggtttg gctttttcag cgtgccgcgg taatacgcca
     3961 tctgcggata ttgatggccg ctgagcatca aataaggata gcttttgtcg tcgcggaaaa
     4021 ggatattgta tttcggcgac aaggctttga tgaggttgtt ttcgagaatc agggcttcgg
     4081 cttcggaacg cgtgatggtg gtttcgatat gatgaacctg ttttaccatc aatgcgatgc
     4141 gcggcgaatg gtcgtttttt tggaaatagc cggacacgcg ccgcttgagg ttgaccgctt
     4201 tgccgacgta taagactttg ccgccttcgt caaaaaaacg gtacacgccc ggcagcttgg
     4261 gcaggttttt gaggaagagc gggatatcga attgttccgg agaacggttt tctgtattca
     4321 tatcaattat ttgaaaaacg gcttcagacg gcatatcggc agcggcagcc ttatccgttc
     4381 ggaaaagctg tcgggtggtt atatgcggtc aatcgatttt tacccgtttc aggcgcaagg
     4441 cgttgctcaa cacggaaacc gagcttgccg ccattgccgc gcctgcgatg acagggttta
     4501 aaaaaccgag cgcggcaagc ggaatgccca aaatattgta gaagaaggcg aaaaacaggt
     4561 tttgcttgat gtttctcaac gtcgcccgcg ataccgatag ggcatcggcg agttggttga
     4621 ccgaatgttg catcagcgtg gcggatgcgg tgtgttcggc aacgtccgcg ccgcctttca
     4681 tggcgaagct aacgttggcg gcggcgagcg caggtgcgtc gttgatgccg tctccgacca
     4741 tcgccacggt tttgccggcg gctttgagtt tctgcacttc ggcggctttg tcgcgcggac
     4801 tcatattacc gaaggcgtgt gcgatgccca gttgtttggc gacgtattcg accgtgcctt
     4861 ggttgtcgcc gctcataata tagacatcga tattgtgttt tttcagacgg cctatggctt
     4921 cggcggtatc ggctttcaac gcgtcggcaa gtgcgaatgc gccgatgggt ttgttatcga
     4981 ctgagaccgc aacaatgctt gcaatatccc aaacgccgtc taaaaacttc ggcaaggcca
     5041 gttcggcaaa ttcggctttg cctgctttca ccaaacccac gccttccact tcggcggtaa
     5101 tgcctgcgcc gacaacggtt tgtgcgtttt gtgcggcggg aatgtccaaa ccgcgcgctt
     5161 gggcggcgga gacgatggca cgggcgagcg gatgggcggc gttttgttcg acggcggcgg
     5221 cgatgcggta caaagcgtct tcgtcaaagc cgctgtcggg aacgcaataa acggcggcaa
     5281 cctgcgggct gccttcggtc agcgtaccgg ttttgtccaa cacgacggca tcgacgtggg
     5341 cggcttcctc cattgctgcc gcgtctttaa accaaatacc gtgtttcacc gctttgccaa
     5401 tgccgaccat aatcgcggcg ggtgtagcca gaccgagcgc acacgggcag gcaatcacca
     5461 aaacggcgac ggcgtgcatc agcgcaaccg tccaatcgcc cttaatcagc caagtaacaa
     5521 taaaagtcaa caacgcgatg cccacgacgg caggcacgaa caccgccgcc gccttgtccg
     5581 ccacgcgcgc aatcggtgct ttactgcctt gcgcttcaga gagtgcgttc atcatgtcgc
     5641 cgagcagggt ttggctgccg agctgcgcgg cgcggtacac cacgctgcct tcagtcatca
     5701 gcgcgcccgc caatactttg ccgcctgcct ttttctcttc gggattggat tcgccggtaa
     5761 gatggctttc gtccgcccag ccgctgccgc tttctatgat gccgtcggcg gcaatgcgtt
     5821 cgccgtgatt ggcgcggatt aggtcgccga tttgcacttg gtcgatgggt agctgccgcc
     5881 attcgccatc gcgttgcacg ttgacttggg ttggcgtgag tttgagcagc aagcccaagc
     5941 tgttcaggct ggattttttg gtgcggtgtt ccaaaaattt acccagtgac acaaaaccaa
     6001 tcaccattat gcctacttca aaatacacat gcgccatacc gtacgccgcg tgcgggctga
     6061 aaaacagcat atagacggaa tacaggtaaa tcgagaccgt gccgatggta acgagtacgt
     6121 ccatattcgc cagcccgcct ttaatgctcg cccacgcgct tttgtaaaat ggcaccgcca
     6181 gccaaagctg caccacactc gccaatgcaa actgcaacaa gggcgacaac atccaatcat
     6241 gccgtgtcca attcagccct tttagcatca tccctaccat accgataagg aacgggatat
     6301 tgatggccag caaaagccac aacctccagc cgatatggtg ttctgcttcg ggttgcggca
     6361 atgtatcttc cgttttttcc ttcgcgccgt aaccggtttt ctcaatgatt ttggcaatgt
     6421 cggctactga ggttttgctg tcgtcaaaca ctacctgcgc ctcttcgctg gcgaagttta
     6481 cccccgccga ttcgacaaaa tcttttttgt tcaacacttt ttcaatgcgc gaagcacatg
     6541 cctggcaggt catgccttcg atttggaaac ggactttttg ttgcataagc gtgattcctg
     6601 ctttgatatt cagacggcgg tcaaggctgc gtgatgccgt ctgaacgccg gtatggtcga
     6661 aatttctcaa acgcgccgat aattcgagta gaatccgaaa acttgacaca gcaaaggaaa
     6721 aacaatgtcc caacaccgca aactgattat tttgggttcc ggccccgccg gatacaccgc
     6781 cgccgtctat gccgcgcgtg ccaatttaaa ccccgtcatt attacaggta tcgcgcaagg
     6841 cgggcaactg atgaccacca ccgaagtgga caactggcct gccgatgccg acggcgtgca
     6901 agggacggaa ttgatggcgc ggtttctcgc ccacgccgaa cgtttcggaa cggaaatcat
     6961 ttttgaccaa atcaacgccg tcgatctgca aaaacgcccg tttacactca aaggcgatat
     7021 gggcgagtac acttgcgatg ccctgattgt cgcaacaggc gcgtccgcca aatacctcgg
     7081 tttgccgagt gaggaagcgt ttgcaggaaa aggcgtttcc gcttgtgcca cctgcgacgg
     7141 tttcttctac aaaaaccaag atgttgccgt agttggcggc ggcaatacgg cagttgaaga
     7201 ggcactctac cttgccaata tcgccaaaac cgttaccctg atccaccgcc gcagcgagtt
     7261 ccgtgccgaa aaaatcatga tcgacaaact gatgaaacgc gtggaagagg gcaaaatcat
     7321 cctcaagctg gaaagcaacc tgcaagaagt actgggcgac gatcggggcg taaacggcgc
     7381 attattaaaa aacaacgacg gttctgagca acaaattgcc gtcagcggca tttttatcgc
     7441 catcgggcac aagccgaata ccgatatttt caaaggtcag ttggaaatgg acgaagccgg
     7501 ttacctgaaa accaaaggcg gcacggcgga caatgtcggc gcaaccaata tcgaaggcgt
     7561 atgggcggcg ggcgacgtaa aagaccatac ctaccgtcag gcgattacca gcgcggcttc
     7621 cggctgtcag gcggctttgg acgcggaacg ctggctgggc agccaaaaca tttgaatgcc
     7681 gtctgaaacc gaaattttcg gcggaatgca aatttttcgc accaaatcct tgattccgtc
     7741 ggcaaacccc aatataatgc ctgctctttg aaccttgcct tctgctttcg catggcagaa
     7801 ggctgttttt accatccata aggagataac atgcgtcatt acgagatcgt gtttatcgtt
     7861 catcctgatc aaagcgagca agtgcctgct atggttgaac gttacaaaac catgattgct
     7921 gaagcaaacg gtaagattca ccgtttggaa gattggggtc gccgccaact ggcttacccg
     7981 attaacaaaa tccataaagc ccattacgtt ttgatgaaca tcgaaaccac tcccgaagtg
     8041 gttgaagagc tggaaaccgc attccgcttc aatgatgccg tattgcgcca tctaaccatc
     8101 aaaaccaaac acgccgttac cgaagcatcc cctatgttgg gtggtgaaaa ggctaagaac
     8161 ctgttgagcg gcgcgtctga agaagcggtc gcccaataat tgggattcaa taatcttgtt
     8221 tcgcttgccg tgttaattga aaaggttttc cctattcgat atacgcctgc cggaatccct
     8281 gttttagata ttattttaaa gcacgaatcg tggcaggagg aaaacgggca gcaatgcctt
     8341 gtccaattgg aaattccggc acggatttta ggcaggcagg cggaagagtg gcagtatcgg
     8401 caaggtgtat atgttcacgt cgaaggtttt ttagctcaaa aaagcagacg ttcccttatg
     8461 ccgatgctca ggatacaaaa tattcaagaa tataaaggtt aaacgacaat ggctcgtcaa
     8521 tcattcaaac gtagaaaatt ctgccgtttc acggctgaaa aaatccaaga agtcgattac
     8581 aaacaagttg atttgctgaa agactttatc tctgaaaacg gtaaaatcat tcctgcacgc
     8641 atcacaggaa cgaaggcatt ctaccaacgc caattggctg ttgccgtaaa acgcgcacgc
     8701 ttcctggctc tgctgcctta caccgaccaa cacaaataat tttggagatt gaatcatgca
     8761 aattattctg ttagaaaaaa tcggcggtct gggcaacttg ggcgacatcg taaccgttaa
     8821 aaacggctac gcccgcaact ttctaattcc cgcaggtaag gcaaaacgtg cgaccgaagc
     8881 gaatatgaaa gagtttgaag cacgccgtgc cgaattggaa gccaaacagg ctgaaatttt
     8941 ggcagatgcc cgagtccgtc aggaaaaatt ggacggtcaa accattaccg ttgcacaaaa
     9001 agccggtgtg gacggtcgcc tgttcggttc cgtcaccaat gctgatattg cggctgcaat
     9061 cgttgctgcc ggcatcgaag ccgtgaaagc aaatgtacgt ctgccgaacg gtcctctgaa
     9121 agccgttggc gagtacgaag tggaagtggc tttgcacact gatgccgttg ctaaaattac
     9181 cgttgccgtc gttgccgcaa ccgagtaatt gaaattatca gatgccgtct gaaaccctgc
     9241 cgattgtcgg aatgtggttt cagacggtat ttgttttgtt tttcgtatat tctgcaaaaa
     9301 tcgggatggt tgtttttata gtggattaaa tttaaaccag tacagcgtac ctggcttcaa
     9361 tatactcaat aggataggtc tcttcctaac cgaaaaaata attgtctttt cccttgcaaa
     9421 taattgaaat caacagatta ctaaaacaca gccaaaatat tcaattgaca aaaacttttc
     9481 tgattttgag aaatctgtga aaatcagggg attatttatt ttgaaaatgg tatcgggcat
     9541 aaaatttggc tgtgtttcaa tatggtgctg ataacatact ttgtttcaaa atgttcaata
     9601 ggataacttc ctgattgacc gaaaaataat ggccttttcc cttgcaagca gttgaaatca
     9661 acagattgtc aaaatacatc ctaaaaattt aataacttat atatatgatt gcaaaggtta
     9721 cggcttgtat gccggtcatt atggcggaaa taccgtttgg gaggcagccg catcgcgtgg
     9781 aatcggcacg gcaggcgatg cgccgatgcc ttcgggcgtg aaggcggggc aatcggcagt
     9841 ttggaaaaag gcaatggaat ctgtgggcag aagaagttaa gggtaaaagt cgggcgcacg
     9901 gtcaggttgt ctgtcctttt gtcggaacag gagactgggc agctttgtcc tgattaattg
     9961 cttgtgcaaa cggtaaaaag gttttttcgt gtgttttaaa gcaggttgta cagcattttt
    10021 ccggcaatca aaagcaacat aatgccgaag gattttttga gtttggcaga agaaagtttg
    10081 tgggcggttt tgacaccgag cggggcaaag gcaatggttg ccgcgctgag gacggcgacg
    10141 gcgggcaggt aaaggaagcc cagtgaccct tcgggcaatc ctgcaatatt caggccgttg
    10201 agcagatacg atattgcgcc ggagagtgca atcggccagg caaggccgga tgatgtgccg
    10261 atggctttat gggcggggaa gccgcagtgg attaagaagg ggacggaaag tgaaccgccg
    10321 cctatgccga cccagctcga cattgtgccg aacagtgtgg aaaccgcagt cagtccgggc
    10381 agtccgggca gcgggcggga tgccgtctga gggtcggtat gcagtgtttt gaatgcgacg
    10441 gcggttaaaa acaggatgaa gaaaatttga agcccgaacg ctgggatata ttttgcggag
    10501 agtgcgccag cgaatacgcc gaataccata cccggcatca tcgtaaatac ggttttccag
    10561 tcgaccgcct gttttttgtg ctgccccagc atactggaaa aggcggtgaa gaccatgacg
    10621 gcgaaggatg tgccgacggc gaggtgttgc gcgtaaggat gttgtgccaa accctgcaaa
    10681 tcaagcaccc ataaaacgac agggacaatc agcgtgccgc cgcctacgcc gaacaggccg
    10741 gcaataaaac ctgccgcact gcctacggca agcaggatta agataatgtc ccaatgccac
    10801 atttaatccg cctttctgta ttttttccag aatcggcgtg ccgccataat gtagccggac
    10861 aggctgtatc cgagaaagaa caggaagagg acgagcgacg gttcccaagt gaccagaagc
    10921 agcagtaaga cggcaagcag cattccgaca aaggggactt gtctgcggat gttgatttct
    10981 ttaaaactcc aaaaagggat ttggacaatc atcgacaggc cggcaaacag tgtgatgccc
    11041 aatgcccacc agtggacggc ggggaatttt tcgacgctgt ggttgaccca aatcagcccg
    11101 acaatcagcg cggcggcagt cggactgggc acgccgataa accagcgttt gtccaccttg
    11161 ccgatgagcg tgttgaacag cgcaaggcgc agggcggcgc aggcgcagta gatgaaggcg
    11221 acggaataac cgattttgcc gaactgccaa agctgccatt tgtaggcaat cagagcggga
    11281 gcaacgccga agctgaccat atcggcaagg ctgtcgagct gctccccgaa cgcgctttgg
    11341 ctgttggtca gccgcgccac gcgcccgtcc ataccgtcca gcaacataga gatgaatacc
    11401 gcgatggcgg cggtttcata acgtccgtgc atggattggg tgattgcgta aaatgcggaa
    11461 aacagcgcgg cgatggtaaa ggagttgggc agcaggtaga tactgttttg acgcagggag
    11521 cgttgcgggg tgttggtcgg ggtttccata gctgaagtgt ctcacggacg tggagaatcg
    11581 ataggggatt ataacccaaa ccattatcga acaatacgcc ctttaggatg aagccctcgg
    11641 aggcggtttg tttttctgat ggcggatgct ttgcggggcg gtttgactct gcctgccacc
    11701 gggtgtgttg tttcggattt ttgccggatg gatattgttg ccgggcattt ctgggcttgc
    11761 cggaaggtat cggggggagg gcatcggaat atgcggcgca ccgttcaaac cggggcggcg
    11821 tttccctatt ttcagggggt tgcaggtttc aacaaattga aagatatttt ttgcatttcc
    11881 gaccgtatgc tgattttgaa gcgggataaa ttataggaca aggtttttcg cccgcggcaa
    11941 ggattgcccg caatgccgtc tgaaaacaac ggcttgccgc taaggcggcg gtatgcggat
    12001 ttgcgctgcc gaaatcggtt tgccataccc ggcggtgcaa tctgcgggga gggataagcc
    12061 gataaggcgg gacttatcct gttttttgtt tataatgcaa cagaatttgg acgaaccgtc
    12121 gggaatttat catgatgaaa aagatactgg ccgtatcggc actatgcctg atgactgcgg
    12181 cggcacgggc tgccgatact tacggctatc tcgccgtttg gcagaatccg cagaatgcaa
    12241 acgatgtttt gcaggttaaa accacaaaag aagattcgac gaaaagcgaa gcgtttgccg
    12301 agttggaagc tttctgcaaa ggtcaggaca cgcttgcggg cattgccgaa gacgagccga
    12361 ccgaatgccg gtcggtcgtg tcgctgaaca atacctgtgt cgcgctggca tacccgaaag
    12421 ccttgggcgc gatgcgcgtt gaaaacgccg ttgtgattac ttctccgcgt tttacgagcg
    12481 tttatcaggt cgcactcaac cagtgcatca aaaaatacgg cgcacaggga caatgcggct
    12541 tggaaacagt gtattgcacg tcttcttctt attacggggg gaactgtgcg ctctttgatt
    12601 caaaatctca aataaaacgg gaaatgccgt ctgaaagatg ttcagacggc atttctatat
    12661 cgacggtcag gattctttcg gatcgggcag caggctgttc aacataatgg caagtacggc
    12721 gcacaagccc acgccggcaa agctgaagct gcccaatttg agcgtcatgc cgccgatgcc
    12781 tgtggtcagt accgagctga cgatgaccag gtttttcggc agcatcaaat cgactttggc
    12841 atcaatcagg gttttgatgc ctaaagaagc aatcgtgccg aacagcagca gcataatgcc
    12901 gcccattact ggcatcggaa tggaagccaa aaacgcattg aatttgccga aaaacgccat
    12961 gcagacggca aaaaccgccg cccaagtcat gatgacgggg ttgctgtttt tggtaatcat
    13021 caccgcaccc gttacttcgc cgtaggtcgt aaccggcggg ccgccgatca gacccgcaac
    13081 gcatacgccc aaaccgtcgc ctgcaagggt tttgtccaag cccgggtctt tcgtatagtc
    13141 tttccccgtc acattgccga ttgccatgat gccgccgatg tgttcgatgg cgggggcgac
    13201 ggcaacgggc agcataaaca gtgcagcctg ccagttgacc tgaggcgttt caaaatgggg
    13261 aacggcgaac cagggcgcgt gtgcaatgct tgccgtgtcc accagtccca tcagcagtgc
    13321 caaaacataa cccgaagcga caccgatcaa gataggaatc agcttcatca tcctgctgcc
    13381 gaaaaccgat acgatggcgg taacggcaaa ggtaaagccg gaaagaatca gcgaatcggt
    13441 atagtcgatg acctgtttgc cgtccgcctg acccattgcc atgctgcttg ccgccacggc
    13501 aacagacaga ccgatgacca tgatgacggg gccgatgacg accggaggca gcagtttgtg
    13561 taccgccgcc agtccgcgcc aacggatcag cgcggcaaac acaaaataca taaagccggc
    13621 ggcaaacagt ccgaacatgg tggaaggcag cccccattcg ccgacggagt agataatcgg
    13681 tgcgataaag gcaaacgaag aaccaagaaa aatcggcact ttgcgtttgg ttgtgatttg
    13741 gaacagcagc gttcccaagc ctgcgcccaa aagcgcaaga gccggattca gaccggtcag
    13801 caggggaacc agcaccattg cgccgaatgc cacaaataaa atctgcgcgc cggaaacggc
    13861 aagtttcagt tggttcatac catcttcttt caatcggttc aaacggcacg gattatactg
    13921 gtttgccgag ggtctgcgta aaaatacata tggcgggcaa cagatatgcc gtctgaaagg
    13981 caatgtctgt ctgttgcagg atgaaagcag gagaaatgga gtataatccg acactttcca
    14041 tattcaatcc gcaagccgtc cggcttcctt atttcagacg gcattttccg gtaatacggc
    14101 atgacagaaa aatcccataa aaaaacagca aaaggcaggg cgggaagccc gtccccaacg
    14161 tccgcacgca acaagaaagc cgacaacggc gcacggggca ataaagtttc cgagcggctc
    14221 aaagcggtca aagagttgca gaaaaccgaa accaaaaagg cgcgtcccga acatgtcgtc
    14281 aaccttatcg gcgacgcact gtggctgatg ggtttggcgg caaccttgta tttggcgatt
    14341 tccctgatca gtttcgatat gggcgatccg tcttggtcgc acagttcgcc ggttgtggaa
    14401 gatgtcgcca attggggcgg actgttcggc gcgtatgttg ccgatgtcgg ctattatctt
    14461 ttcggctggt cgttctggtg gtggatagcg gctgcctgcg tcatgctgta taaaaatttt
    14521 cgtctgcacg caaaacagac ggaaaacgag gcatacaacc acaaaatcgc tgccgccgcg
    14581 ctgtttgtcc tgacggtctt cagccccgtc ttggagtatt ttgtgctggg cggaaaatat
    14641 gccgactccc tgcctgtcgg agcaggcggt atggtcggca tacgcgtcgg cgcagtgttt
    14701 gcgtggctgc tggggaaatc gggcagcctg ctgattatct tggttgttct gctgttgtcg
    14761 ttgtccctgc tggtgcagat ttcatggctg gaatttttga acggtgcggg cagggcggtt
    14821 caaaaccgcc tgagtgcctt atccggcaag gtcatggctt tgggaaaacg ccggccgaat
    14881 accaaaacag acggtgtcga tacccaaaat acacggcgca tggtaaaaga agccaagaat
    14941 attacggcca aacccgttgc cttgcccgaa ggcagcagca gcaaccgcaa atccgtcgcg
    15001 gtttccgtcg cgccgccgcc caaaattcag gtttctctgt ttgaagatga cgaacctcgg
    15061 caggcgggcg aataccacaa gcctacattg aacctattgc ggattcctga cagcgaaccc
    15121 gtcagcatca atcccgccga attggagcgc actgccgaac tgatcgaatc caaactggca
    15181 gaattcggca tcggcgtaca agtcgtatcc gccacatccg gtcccgtcat cacgcgctac
    15241 gaaatcgaac ccgcgcaagg tgttaaaggc agccaaattg ttgccttgtc gaaagatttg
    15301 gcacgctcta tgtcgctgca gtccgtgcgt atcgtcgaaa ccatcgcagg taaaaacacg
    15361 atgggcatcg agttgcccaa cgacaaacgc caagacgtga tgttgagcga aatcttgtcc
    15421 tcgcccgtgt ttgccggagc caaatccaag ctgaccgtcg cgctgggcaa agacattgcc
    15481 ggtacccccg ttgtcggcga cttggcgaaa atgccgcacc ttttggtcgc cggtatgact
    15541 ggttcgggca agtccgtcgg cgtgaacggc atgattatgt ctatgctttt caaagctacg
    15601 cccgacgaag tccgcttcat tatgatagac ccgaaaatgc tcgagttgag catttacgac
    15661 ggcattccgc acctgctctg tcccgtcgtg accgatatgc gcgaagcagg gcaggcgttg
    15721 aactggtgcg tcgccgaaat ggaaaaacgc taccgcctgc tttcccacgc cggtgtacgc
    15781 aatctggaag gcttcaacca aaaagtcgaa gccgcaaaag cggcaggcaa gccgctgctc
    15841 aatccgttca gtctgaaccc cgacaacccc gagcctttgg aaaaactgcc gatgattgtg
    15901 gtcgttatcg acgaacttgc cgacctgatg atgaccgaac gcaaagccgt cgagcagcaa
    15961 atcgcccgtc tcgcccaaaa agcgcgcgcc gccggtatcc atatgattgt cgccacccaa
    16021 cgtcccagtg tcgatgtcgt taccggcctg attaaagcca acatcccgac gcgtatggcg
    16081 tttaccgtgc aaagcaaaat cgacagccgt accatcctcg accaaatggg cgcggacgaa
    16141 ctgctcaaat atggcgattc gctgttcctt cagcccggca gtgccgaacc gacccgcctg
    16201 caaggcgcgt ttgtttccga tgatgaagtc caccacgtcg tcgctttcgt caaggagcag
    16261 gcaccggcaa attatgtaga agggctgctt acaggcgaag cggcacagga aacggcaaat
    16321 atcgtcagcc ccaatgcaga cagtgacgaa ttgttcgatc aggctgtcgc ctatgttttg
    16381 gaaagcaaaa aaacctccat ttcgtctttg cagcggcagt tgcgtatcgg ctataaccgc
    16441 gcggcaaacc tgatggaggc actggaaaat gcaggtgtcg tttctccctc cgatcttaac
    16501 ggcagccgta aaattttggc gcacaaggac catttatagc ccatatcgca aaatgccgtc
    16561 tgaacggtgg aattggcgtt tcagacggca tattatgttt caggcgaaac attgtgatat
    16621 acttgccagc taaaatttcc cctttgcggc aatgcggttc aaatatcgta ccgttgcgct
    16681 gtttgcttcc ccatgtaggg aagaaagttt atcattttat caacacaaca aatttaaggg
    16741 cttatgatga gcgtaactgt tgaaacttta gaaaatctgg aacgcaaagt agtgttgtcc
    16801 ctgccttggt ccgaaatcaa cgcagaaacc gataaaaaac tgaaacaaac ccaacgccgt
    16861 gcaaaaatcg acggtttccg tccgggtaaa gcacctttaa aaatgattgc ccaaatgtac
    16921 ggtgcgagcg cacaaaacga cgtgatcaac gagctggtgc aacgccgctt ccacgatgtt
    16981 gccgttgccc aagagttgaa agtggcaggc ttcccccgtt ttgaaggcgt tgaagaacaa
    17041 gacgataaag aatctttcaa agttgccgcc atttttgaag tgttccccga agtcgttatc
    17101 ggcgatttgt ctgcacaaga agttgaaaaa gtaaccgctt ccgtcggtga tgccgaagtg
    17161 gaccaaaccg tagaaatcct gcgcaaacaa cgcacccgct tcaaccatgt cgaacgcgaa
    17221 gcccgaaacg gcgaccgcgt catcattgac tttgaaggca aaatcgacgg cgaacctttt
    17281 gccggcggcg catccaaaaa ctacgccttc gtattgggcg caagtcaaat gctgcctgaa
    17341 tttgaagccg gcgtagtcgg catgaaggcg ggcgaaagta aagacgttac cgtcaatttc
    17401 cctgaagact accacggtaa agacgttgcc ggtaaaactg ccgtgttcac cattacgctg
    17461 aacaacgttt ccgaagcgac tctgcctgaa gtcgatgcag attttgcaaa agccttgggt
    17521 attgcggatg gcgacgttgc caaaatgcgc gaagaagtga agaaaaacgt aagccgcgaa
    17581 gtggaacgcc gcgtaaacga acaaaccaaa gaatccgtaa tgaacgcgct gctcaaagcc
    17641 gtagagctga aagcacctgt tgctttggtc aatgaagaag ccgcacgctt ggcaaacgaa
    17701 atgaaacaaa attttgttaa ccaaggtatg gctgatgctg ccaacttgga tctgcctttg
    17761 gatatgttca aagaacaagc cgaacgccgc gtgtctttgg gtctgatttt ggccaaactg
    17821 gttgatgaaa acaaactgga accgactgag gagcaaatca aagccgttgt cgccaacttc
    17881 gcagaaagct acgaagatcc tcaagaagta atcgactggt actacgcaga gccttcccgc
    17941 ctgcaagccc cgacttcttt ggcgatagaa agcaacgtcg ttgatttcgt tttgggtaaa
    18001 gccaaagtaa acgaaaaagc tttgtctttt gacgaagtga tgggcgcgca agcctgatta
    18061 tcctgaaaat gccgtcttag ggcagttttt gaaagcaccg aagccgcttt taaagcccgc
    18121 tttggtgctt ttacatcatg aaaggagacg aaatgtcttt tgataactat cttgtcccta
    18181 ccgttatcga gcagagcggt cgcggtgagc gtgcattcga tatctattcc cggcttttga
    18241 aagagcgcat cgtattcttg gtcggcccgg taaccgacga gtccgccaat ctggtggttg
    18301 cccaactgtt gtttttggaa agtgagaatc cggataagga tattttcttc tatattaact
    18361 cgccgggcgg ttcggtaacg gccggtatgt cgatttacga caccatgaat tttatcaagc
    18421 ccgatgtatc gactttgtgc ttggggcagg cagcaagtat gggcgcgttc ttattgtcgg
    18481 caggcgaaaa aggcaaacgt ttcgccctgc ccaacagccg gattatgatt caccagcctt
    18541 taatcagcgg cggcttgggc ggtcaggcat ccgatattga aattcacgca cgcgaactgt
    18601 tgaaaatcaa agaaaaactc aaccgcctga tggcgaaaca ttgcggccgc gatttggcag
    18661 atttggagcg cgacaccgac cgtgataatt tcatgtctgc cgaagaagca aaagaatatg
    18721 gtttgatcga ccaagttttg gaaaaccgcg cttctttgca gctttaaacc ttgtgaagta
    18781 gacaaatgcc gtctgaaaac agttcagacg gcatttgtct tatagggact gtattgggat
    18841 gattttgttc tggttgcgtt tggagccgtt ttcgccgtag ttggttttga catactcttc
    18901 aacaatagcc aaaaattccg ttgcaatgtt gtcgcctttc agcgttactt tgcgttcacc
    18961 atctacataa acaggcgcga cgggtgtttc ccccgtaccg ggcaggctga tgccgatgtc
    19021 ggccaatttg ctttctccgg gcccattgac aacgcagccc attacggcaa cgttcaggga
    19081 ttcaacccca ggataaaggg tacgccatat agacattttt tggcgcaggt aattttgaac
    19141 atcttgtgcc agctcttgaa atacggtact ggtggtacgc ccgcagccgg ggcaggcggt
    19201 aaccatcggc gtaaacgaac gcaatcccat agtttgtaaa atctcttgcc cgacgacgac
    19261 ctcctgagta cgcgggctgc caggttccgg agtcagtgaa atgcggatgg tgtcgccgat
    19321 tccttcttga agcaagacgg ataatgccgc cgttgatgcg acaatgcctt tgctgcccat
    19381 accggcttcg gtcaaaccca aatgcagcgg ataggcgcaa cggctgccca gttcgcggta
    19441 aacctgaatc aaatcctgaa ccgcgctgac tttgcacgac aggatgattt tgtcttcggg
    19501 cagtcccaat agaacggctt tttcggcaga ttccaaagcg gagacaatca gtgcttcctt
    19561 cgtcacttct tcgggcggtt tcggcgcgga agaagcgagg ttggcatcca tcatacgttt
    19621 ggcgaggctt tgatccaaag aaccccagtt tacgccgatg cggacggctt tatcgttttc
    19681 agcagcagtc cgaatcataa aggcaaattt ttcatcgcct tttacgcctt tgccgacatt
    19741 gccgggattg atgcggtatt tggacaatgc tttgccgcat tctggaaatt ccgccaacag
    19801 gcgttcgccg ttgaagtgga aatcgccgat aagcggtgtg gcatagccca tatcgtccaa
    19861 gcggcggcgg atttcggcaa ctttggacgc ggcttcgggg ctgttgacgg taatacgcac
    19921 catttcggat ccggcgtcgc tcaattcctt aatctgcaat gcggtggctt ttgcatcggc
    19981 agtgtcggtg ttggtcatag attggataac gacgggtgct tctgaaccga cggtaatatg
    20041 atcgatgcgg acttgatgcg tcttgcggcg ttggagtgtg ttcatatggt ttgaatctgt
    20101 ttattgaccg atgaggacgg tttgcaattc ttccgagtag gggaaattcg cctgcaattg
    20161 tgcttcatat tcgtatgccg cctgtgcgtt gccgagggct ttggcaattt tccagcctag
    20221 cagcaaatca tcggcctgaa ggacttctac cctgctttgg tattttttaa agtagtaatc
    20281 ggcatcgccc aactgcccgg ccagcatttt ggtgcgcgcc agttctttaa atgcgggtgg
    20341 gaactgcggc tgggcggcga gggaacgttt caaataggct tccgccaatc cgaattgccc
    20401 ctgttttgcg ctgcatatgc ctttattcag gttggcaata taaggggtcg ggtaggtggg
    20461 gtcggccagg gctttgtcga aatatgccat agattcggca gggcggttga gcctgccgca
    20521 caggaaccag ccgtagttgt tgttgatttc ggcactgtcg ggtttgatgg agagggcttg
    20581 ccggaaactt tcctgcgcct tgtcgttaac tttcaggtat tgatagattt cggcacggac
    20641 cagccaggca agctcgtttt tagggtctga tttcaaggcg tcttcaatac ttgccgtcgc
    20701 ctgacggtag tcctgaccgc gcatatattc cattgccaac tgggttttga tattggaaac
    20761 ctgattggct ttttctgccc gcgaggggcg gtaggaagtg ctgcacgcgc ccaaggcaag
    20821 gacaagtaat aaagagattc gtttggatgg cttaaaaggc ataattaccc ctgttgtccg
    20881 attaaaatct gctgccattt ttgttggcgg cgcgttttat cctgaacctg ccccgccaac
    20941 tgtccgcagg cggcatcgat gtcgtcgccg cgcgtttttc gtacggtaac gacaaatcct
    21001 gcctgctgca aaatatcgcg gaacacacgg atgttctcat tgctggagcg ttcgtatccg
    21061 gagtttggga agggattgaa cggaatcaga ttgaacttgc agggaacatc tgtgaccagt
    21121 tcgatcagtt cgcgcgcatg ttgcgcctta tcgtttattc cgtccaacat gacgtattcg
    21181 aaagtgatga aatccctggg tgctttgacc agatagcgtt ggcatgcggc catcaattct
    21241 ttcaagggat attttttgtt caacggtacg atttggttgc ggacttcgtc attggaagcg
    21301 tggagggaaa ccgccaaagc caccggcatg acatcgcgca acctgtccat ttggggaacc
    21361 atacccgaag tggaaacggt tacgcggcgg cggctcaaac cgtagccgtg gtcgtccagc
    21421 atgatgctta aggcggtaac gacattgtcg aagttcgcca tcggctcgcc catacccatc
    21481 atcactacat tggaaattac gcgttcgttt ttcggtgtaa cacccatcgc tttgtttgcc
    21541 caccacaatt gcccgatgat ttcggcagca gtcaaattgc ggttgaagcc ctgccggccg
    21601 gtcgaacaaa atgtacattc caaagcgcag ccgacttgtg aggaaatgca gagcgtgccg
    21661 cgatccgatt cggggatgaa gacggtttcc accccgttgc ccgtaccgac atccaaaagc
    21721 cattttcgag tgccgtctga agatttttga gacatcatca gcttgggaat ttcgatgcct
    21781 gcctgttcgt tcagtttatg gcgcaacgat tttgccaaat cggtcatttc gttaaaattt
    21841 tgcgcgccgg attggtgcat ccaacgcata acctgtttgg cacggaaggg tttttcgccc
    21901 atatcggcaa aatgtcgggt cagcccttga aggtcgtagt tgagcagatt ggttttcatg
    21961 tgtttgtgtt tttcttaaat caaggcttca aataaaaaat tgcagggcag gcaagtttga
    22021 tttgaagcct tttgcaacag agtcttcaga cggcatggat atattttatg ccgtctgaag
    22081 aggagttggg cggtgtattg tatcaacggg ggcagatttc ggtttggctg aaaaagtagg
    22141 caatttccaa agcggcattt tccacgctgt cggaaccgtg tacggcatta atgctgaccg
    22201 aagtggcaaa gtccgcgcgt atcgtgcctt cggcggcttc ggaaggatta gttgcaccca
    22261 tcagttcgcg gtttttcagg acggcgtttt caccctctaa tacctgaatc ataaccggac
    22321 cgccggtcat aaattcaacc aatccggcgt agaaggggcg gtctttatga accgcataaa
    22381 attcttgcgc ctctttgagg gtaagctgct tcattttggc ggcaacgatt ttcagaccgt
    22441 tctcctcaaa gcggctgtat attttgccga taacgttttt gccgacggca tcgggtttga
    22501 tgatggagat ggtacgttca atcgccatgc tatatcctta tttttactta agaagaatca
    22561 aatcggtatt ctatcaaaaa aataacttat ccgcctgaag ccttgggaga tagggtcagg
    22621 tgcggctgtt ccaaataaac ttcggactgc atttcgacca tccggctggc ggtgcgggta
    22681 aattcttcgg caaaatcgcc ttccgtatag atatggttga catctaccgc gccggtggca
    22741 cacagtttga cccggaaatc gtagagtacg tcaatcagcc aagtcagccg ccgcgcctcc
    22801 gccttttctt gcggtgagag ttgttccaaa cctgaaataa aaaccatttc ataatgttcg
    22861 gccaaataca gatagtcgga ctgtgagcgg gggccgaagc acagtgcgcg gaaatcaaac
    22921 catatggcac ggccggactc ggctttgtgg ggaatctccc gaccatggat ggtgctgatg
    22981 ccggggttca aatcggtaat gcctgtcatt tctttgaaca gttttgccag ttttgcctca
    23041 ttttcttcat tggcaggcgt aaagaaaatc tcggcggggc ggagggtacg cagtcggtag
    23101 tcttcaccgc cgtcaacgtt taagacggtc aggctggact cgatgagcgc gattgtggga
    23161 agaaaactgc tccggttttg accttgcggg tagagttcgg aaggcgcgta gtttgaagtc
    23221 gccaccaaaa caacgccctc gttaagcagg ttttccagca gacggcctaa aatcattgca
    23281 tccgcaatat cgctgacatg aaattcgtca aaacacaata cgcgggtttc tttggcaatc
    23341 tcggcggcaa cggatttcaa cgggttgctt tcgcttttca gggttttcag cctctggtgg
    23401 atttctgcca taaaggcatg aaagtggacg cggcgtttgc ggcggtaagg gaggcagccg
    23461 aaaaaagcgt ccatcagaaa gcttttgccg cgtccgaccc cgccatagaa ataaagccct
    23521 ttggggactt gcggggaacg caaactcctg cctaaaaaac ggtttctttt gcgtttgaac
    23581 atcatcaatt cggtccaaag ccgatcgagg tgttcgatgg cggctgcctg cgcgtcgtcg
    23641 cggatgaagt tgggcagttg tgaggcagcc tgataccaag tcagcgggct gtgattttca
    23701 aacggcgggg ctttaaaaag ttggtctcta ttactcatat ttaaccttgt tatacttctt
    23761 gcggcggaga aagaaaatgc ttaccgtcgg tattataaat gatttccttc aaacttggcg
    23821 ttttccgtgg aaaagaataa tgacgcgcac aagggggggg tatgagcaaa ggcggcgttt
    23881 caatatggtg cagatatgag ctcaaatgcc ttttatagta gattaaattt aaaccagtac
    23941 ggcgttgcct cgccttgccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga
    24001 tttttgttaa tccactataa tccgtagagt cggcttcttc cggctcagac ggcatacgtt
    24061 tatgccggca cttcttaccg ccctcgtgcc ggctatccca aaactgcttt gaacactgaa
    24121 cgctctttgg tttacttcaa aaacgcggcg tgataagcaa tatgctcgcc aataaaactg
    24181 gcgatgaagt aatagctgtg atcgtagcct ttatggaaac gcacatcgac cggctggttt
    24241 gccgcacggc aggtttcgat aaaatcttcg gtacgcaatt gtgtcggcaa aaactcatct
    24301 tccaagcctt gatcgatgcg cataccttgc actttatagc cttgttgaat gagtgagttc
    24361 gcatcatatt gctgccattt ttcacggtct ttccctaaat aagcagtaaa ggctttttct
    24421 ccccacggca cgaggcttgg cgataaaata ggcgaaaagg cagaaacact ttgataacgt
    24481 tcctgattcc gcagcgccaa taccaatgcg ccgtgtccgc ccattgaatg ccccataatg
    24541 gaacgtttgc cgttggtagg aaagtgtttc tcaatcagac ggggcagctc gttcaaaatg
    24601 taatcataca tttgataatt cgccgcccaa ggctgttcgg tcgcattcaa ataaaagcct
    24661 gcaccctgtc ctaaatcgta agcatcatcg ttcggcactt gctctccgcg agggctggta
    24721 tcggaggcca ccacaattac ttgatgttct gccgcataac gctgaaagcc tgatttggta
    24781 atgaaatttt gttccgtaca cgtcaagccg gaaagccaat aaatcacacc aagcggtcga
    24841 ttttctggat tatttggcaa atagacggca aatttcattt cgcattgaag cgtttgggca
    24901 ttgtgtgccc aaacttgttg cgaaccgcca aaaatttgat gttgttcaat cagtttcatc
    24961 gcatacctta gtagtgaata acggcgcgga tcgatttacc ttcgtgcatt aactcaaagg
    25021 cttcattgat ttgatcgagt gtcattgtgt gggttacaaa cggttctaac tcaatgtcgc
    25081 ctttcattga atcttccacc attttcggca gttcagagcg gcctttcacg ccaccaaatg
    25141 ccgtgccttt ccacacacgg cctgttacca actggaacgg acgagttgaa atttcttgtc
    25201 ctacacctgc tacgccgatg ataatggatt gtccccaacc acggtgtgca ctttctaatg
    25261 cttgacgcat tacgtttaca ttgccgatac attcaaaggt atggtcaatg ccccatttgt
    25321 tgatgtctaa caacacatct ttgatcggtt tatcgtaatc gttcgggttc aaacagtccg
    25381 ttgcaccgaa ctgttttgcc aactcgaatt ttgagggatt ggtatcaatg gcgataatgc
    25441 ggccggcttt ggcttgacgc gcaccttgca caaccgccaa accaatcgct cccaagccaa
    25501 acactgccac agagtcgcct tcttgcactt ttgccgtatt atgcaccgcg ccaatacctg
    25561 tagtaacgcc gcagccgagc aaacatactt gttcatggtt cgcttccggg ttaatttttg
    25621 ccagtgaaac ttctgcaaca actgagtatt cactgaaagt cgaacagccc atatagtgat
    25681 agatcggctg accttgataa gaaaaacgcg tcgtgccgtc cggcattaag cctttacctt
    25741 gtgtatcacg cactgagacg cacaagttgg ttttacctga acaacaaaac tcacattcgc
    25801 cacattcggc ggtgtaaagc ggaatcacgt gatcaccggg ttttacgctt gacacacctt
    25861 cgcccacagc aacgaccaca cccgcacctt cgtgtccaag caccacaggg aatacgcctt
    25921 caggatcgct tcctgataac gtaaacgcat cagtatggca cacgccagtg tgggtattgc
    25981 ggattaacac ctcgccttta cgcggcattt ctacgtcgat ttccacaatt tgtaagggtt
    26041 gatttggggc gaatgccacc gccgcacgag atttgatggt tgaatcggct tgtttcattt
    26101 ccattgtctt tcctcttaat tcattgacaa agttgccttg cctgagcaac atagcaacag
    26161 tttacacttt agagttaact ctaaagcaag tgttttttac tgtttttgtt tagaaggagg
    26221 gaaaatgact tatactactg ccaaagccgc cgaaaaaata ggcatctccg cccacaccct
    26281 acgtttttac gacaaagaag gtttgttgcc caatatcgga cgtgatgaat acggtaaccg
    26341 ctgttttacc gataacgatt tgcaatggtt gggcttattg caatgcttga aaaatacggg
    26401 gatgagctta aaagacatca aacgctttgc ggaatgtacc gtcattggcg acgataccat
    26461 tgaagaacgc ctttccttgt ttgaaaatca aatagaaaat gtgaagtgtc aaattgccga
    26521 attaaaacgc tatttagatt tgcttgaata caaattggcg ttttaccaaa aagcgaaagc
    26581 attaggctcg gtaaaagctg taaatttgcc gcaaattcct gaaacggctt agttttagtg
    26641 caataagcag gatttttgtc ctgttcgtaa tagaaatcaa aaatttgttg tttaaaaagc
    26701 ggattgtaag caatgatata gtggattaac aaaaatcagg acaaggcgac gaagccgcag
    26761 acagtgtaaa tagtacggaa ccgattcact tggtgcttca gcaccttaga gaattgttct
    26821 ctttgagcta aggcgaggca acgccgtact ggtttttgtt aatccactat aaaaaaatcc
    26881 acgctctaat ccgttgcttg cttggctcaa ggtattgaag caacggatga agttaaacca
    26941 cagaaaaaac gccgcgtaac agcggcgttt tgatgctaag gtttaattgg cattttcttt
    27001 taaagccaag tcgacccgct cgcgcaattc tttaccgggc ttgaagtggg gtacatgttt
    27061 ttcaggtact tccacacgct cgccggtttt gggattgcga ccgatgcggg caggacgatg
    27121 gttcaaatcg aagctgccga aaccgcggat ttcgatgcgt tgacctcggg caagcgatct
    27181 agtcatggtg tcaaccaaga cttttacgct gtactctacg tcttttgcca gaagatgcgt
    27241 gccgtttttg gcggcaaaca cttctgccaa acgaaccatt aactcagact ttgtcatgtc
    27301 tgcaacctta ttcttgttcg ccggagagtt tggctttcag caggtcgccc aagctggtgg
    27361 tgccggcatt cgcattggcg gcggcattga cggagttcag tgcttcgcgg ctttctttgg
    27421 catctttggc tttaacggaa agtttgatgc tgcggttttt gcggtcaacg gtaacgatga
    27481 cggcttcaac ttcgtcgcct tctttcagtt tggtggtcaa atcttcaacg cggtcggctg
    27541 caaattcgga agcaggcagg tagccttcta cttcgtcaga cagggcgata acagcacctt
    27601 tggcgtcaac agatttcacg gaacctttaa ccaaagaacc tttgtcgttc acgctgatga
    27661 agttgccgaa cggatcgcct tccagttgtt tgatacccaa ggagatgcgt tctttttcca
    27721 cgtcgattgc caatacgacg gcttcgactt cttcgccttt tttgtatttg cgtacggctt
    27781 cttcgccgga ttcggtccag gacaggtcgg acaggtgaac caaaccgtcg atgccgccag
    27841 gcagaccgac gaatacgccg aaatcggtaa tggatttaac cgcgccggag attttgtcgc
    27901 ctttgttgtg gttggcggca aattcttccc aaggattggc ttggcattgt ttcataccca
    27961 aagagatacg ccggcggctt tcatcgattt cgaggatcat tacttcgact tcgtcgccca
    28021 attgtacaac tttgctcggg tgtacatttt tgttggtcca atccatttcg gaaacgtgca
    28081 ccaagccttc gatgccttgt tcgatttcaa cgaacgcacc gtagtcggtc aggttggata
    28141 ctttaccgaa caggcgggta ccttgcgggt aacgacgggt caggccgctc caaggatctt
    28201 cgcccagttg tttcataccc agagaaacgc gttgtttgtc ttggtcgaat ttcaggactt
    28261 tagcttctac ttcttgaccg acttccagaa cttcgctcgg gtgtttcaca cggcgccatg
    28321 ccaagtcggt gatgtgcagc agaccatcga tgccgcccaa gtcaacgaat gcaccgtaat
    28381 cggtaatgtt tttaacgatg cctttgatga cggagccttc ttgcaggttt tccagcaggg
    28441 ctttgcgttc ttcacccaag gtagcttcca gaacggcgcg gcgggacaca acaacgttgt
    28501 tgcgtttttt gtccagtttg atcactttga attcaatttc tttaccttcg aagtgagaag
    28561 tgtcttttac aggacgtacg tcgaccaaag aacccggcag gaatgcgcgg atgctgctaa
    28621 tcataacggt caggccgcct ttgactttgc cgttgatgat gccggacagg atgttgccgt
    28681 tttccatggc ttcttccagg gcaatccaat cggctgcacg tttggctttt tcgcgggaca
    28741 gtttggtttc gccgaagccg ttttcgacgg attcgatggt aacggtaacg aagtcgccga
    28801 ctttaacttc aatttcgcct tgagcgtttt tgaattcagc tacatcaatc agggattctg
    28861 atttcagacc tgcgtttacg gtaacgaagt tttggtcgat tgccactact tcagcggtaa
    28921 tcacctcacc cgggttcatt tcttgcaggg taaagctttc ttccaacagc tgagcaaaat
    28981 tttccataga catatataac tcttttcggt acaccgccaa ggggtgcggg gtgggttggt
    29041 ggatatctgc cgtccttggc agggcagacg ggtacataag gtttcagacg gcatatgggg
    29101 gatttttatg ccgtctgaaa cgtaatttgt gcgattatac ctgaaaattt aaacttcacg
    29161 ataccaatca agtacttttt ttacagtttc ttctatagtc aggcggcttg tgtccaaaag
    29221 cagggcatcg ggctgttgtt tcaggggggc aaccttgcgg tttcggtctg cctcgtctct
    29281 ggcttcgatg tcggacagga tgcgctcgaa tgccaaacct tcgcagggga tgccgatttg
    29341 tttggcgcgg cgttcggcac ggattttgga ttctgccgtc aggaagattt taagttcggc
    29401 ttgggggaag acgaccgatc cggtgtcccg tccgtcggcg accagtcctt tttcggtcag
    29461 aaaatcgcgt tggcgttgca gcagggagtc gcggacttta ggcaactgtg cgactgcgga
    29521 tgcgcccatg ccgatggctt ctgtccggat gccgtctgaa acgtcttcgc cgccgagcag
    29581 gatgcggctg tctgaaaata cggcgggcag tttttttgcc agttcggaaa cgttttcttc
    29641 atcgtgccat cccacgcctt gtttttgtgc atatagggcg gtcaggcggt agagtgcgcc
    29701 ggtatcgaga taatcgtatc ccaatgcggc ggcaacgcgg gcggcaaccg tgcctttgcc
    29761 cgatgcgccc gggccgtcga tggcgatgac tttttgtctg ttcataaggg ggattcctga
    29821 tggtttgggg tatgggtttt gccgtctgaa ggatgtgttt cccgttgggg cggattctac
    29881 ctgttttaaa ggacgattgt ctaagcagac ggaacgccgc ctgcccggaa atatccgaca
    29941 gaagccggca agccgattcg gattccggcg ttgcgggtat tgtggcgggc atcaatgtaa
    30001 tgccgtctga agtcagtatg gctttcagac ggcattaatc ggtgtgaatc agaaaaggtt
    30061 gctgacgaaa atcagcagaa tcaccaacgg cacaagatat ttcacataag caaaccaaat
    30121 attgaccgtc gtatggttgc ctttataaag caattcttcc ttcgcttcat ccttcatcac
    30181 gaaaccgaca aacaatgccg agccaagcgc ggtcagcata aacaagatgt tgccgctgat
    30241 gtagtcgaag gcatcgaaaa tatttttgcc gaacacggaa acgtctttcc acgggccata
    30301 gctcaaaata gacgggatgt tgccgaaaac gtagatggcg gccaatacaa tcgtgattgc
    30361 ggcggtacgg cggattttgg ttttttcctg aatggtcgta atcaatactt cataaatcgt
    30421 cagcgaagtc gtcaacgcgg caatcaggag cagcgagaag aaaatgacgg cgaacacaga
    30481 tcctgcccac atatgcgaga acacaatcgg caaactttgg aataccaaag tcgggccgga
    30541 atcgggggca acgccgaagc tgaagagcga cgggaaaatc ataaagcccg caagtatggc
    30601 gatgatggta ttggtaattg ccgtgataac tgccgtctga accagatttt cgtttttatc
    30661 caaatagctg gacaaggtaa tcatcacgcc gaaacccagg ctcagggcaa aaaatacctg
    30721 ccccaaaacg aagacgaaca gttcggcggt aatcttgctg aaatcaggtt tcagatagaa
    30781 agcaacccct tccattgcgc ccggaagggt aacgttgcgg acgaccatcg cgattaggaa
    30841 caaaaacagc agcggcatca gctattttgc cgctttttca atgccgccga taacgccttt
    30901 gaccaaaatc cattggttca cggcgacaaa aagcagcgta taaaacgcaa tttcccaagg
    30961 gctgtttcaa tgtgttcggc aaagaagcct tttgtaacca caccgtcgat ggggctggaa
    31021 atattcaaat ttcctccaat aatattaacg atatagctga ttacccagcc gccgagtgcc
    31081 atgtaataag ccatgatgcc gaacgcgccg agcagcccca tccagccgac cagtttccaa
    31141 attttagcaa tgggtttgcc gttcatcgga ccgccgaacg catccagcgc gttcacgcct
    31201 ttgcgccgtc cgatgacgtt ttccaccaaa atcatcggga tgccgataac gagcatggcg
    31261 atacagaata aaaacacata cgcgccgccg ccgttttcac cgaccaaata cgggaaacgc
    31321 cacgtcgcgc cgaagccgac agtcgcgccg gcaacagtca ggatataggt taatcggctg
    31381 gatcaggttt gacgattggt atttgaaggg gaagacatat tgaaaccgtg tccgattgag
    31441 ataaagcgga aattctacac gcgtttttta acaggaattg actgattgtt tttcaactgt
    31501 ctagtgattt tccatgtaaa aggcatatat ttcagtgagt atttctgatg agtataaccc
    31561 gatgaggaag aatcggagtt tataaataga ttaatttgtt attcttctac atcggtgtat
    31621 ggaaatgaaa ttttgttaat cacattaatg atagcatttt aataatgatg aaagagaggg
    31681 aatttgaaat aaaaaaccat ctatacaagg ggagaagtat agatggcaaa acacattacg
    31741 gggaaaacgt cttactcata agcctgcttg aacaggcgtt actcagacaa atggattata
    31801 tcgtaaattg attttttgcg ttaaattggg ttaaaccata tatttaatga gtatgtggat
    31861 gaatataaat ggaattcttg gatgttattt tagaatttcg gacaatcgat aaatttccat
    31921 tcgccgggtt tcaggttttc tgtttccaaa tgtgcgaatc gccggcgatg aaggtgttgc
    31981 acgcggttgc ctgcggcggc gaccatacgt ttgacttggt ggtattttcc ctcggtaatg
    32041 gtcaggagca atgcggacgg gctttccaat acggcatcgg cggcgtaaac cgtttcgttt
    32101 tcgtcgtgga gcaggacacc gtttttcagg gtttcgcaaa gcgtttcgcc ggcagggtgc
    32161 ttgagtgtaa cttcgtatag cttgggaatt tttctgctcg gcgaagtcag gttgtggttc
    32221 agtttgccgt cgttggtaat cagcaatacg ccggtcgtat ctgcatccag cctgccgacc
    32281 gcctgcatat cgatgttccg catattgtcg gggaacaggc tgaatacgct gcggtagtgc
    32341 ttgggtttgt gcgaagtttc gtaatcttca ggcttgttga gcatgatgta gaaatagggt
    32401 tcgggaacga cggttactgc ttccccgtca atatccaacg tttcgacgga tgaggaatcg
    32461 atgtctgcat cggtgtcgtc catgcaggtt ccgttgatga aaacataacc gccggcaatc
    32521 agccattggc actgcttgcg gcttcctatg ccttgatatt gcaggtattt gataagtttc
    32581 atgatggtat gggaaatgtg ggatgaaaaa gacaggactg agaaagtcct gtcatgtgcc
    32641 gtatccgaat cagcttccaa tcagtttgac cctctgtccc ggattgatgg tgttcaggtt
    32701 gggattgagc cggcggatgt cgtcgatatg gatattgaag cgcgcggcga tacttttgaa
    32761 ggtgtcgcct ttgcgcgcgg tgtaggatac tttttcaata cgggtttggg caggggctgc
    32821 ctgcgccagg cggagtacct gtcctttttg gatggtgttg cctttgatgt tgttggcgac
    32881 aatcaggtcg gctacactga cgttgtagcg tttggcaatg ttgaacaggg tgtcgccttc
    32941 tacaacgcgg tggatgctgg catggagcgg ggagatttcg gcttcgccgt cggcagcttt
    33001 ggctacgcgc gtttccaaac gtacccggcg tttgccgtcg gcggcaacgg tatgttgcgg
    33061 agatgaggaa atgttgtgtt cggcaactgt gtcaggcgtg ccgatgacgg cagagatggt
    33121 ttcttcagcc tgtcggcgca ggttgtttcg ggcaacaagc tgcatgagtt cgtctgccgc
    33181 ctgcatgtcg ttttggggga cgatctgcgc gagtgttgcg gtttgggttt cagacggcat
    33241 ggcggtctgt ttttcggttt gaggttgcgc tgcggctgtc gcaagggcag gttcggcaat
    33301 acggacaagg ggatcgggtt ccgtacggac ggttttctgc ggcaaaggtg cgacggtaat
    33361 gtccgctgtc tgtgcggcgg cgggtcggat tcgggcaatg ccgacgttca ccgtgcctgc
    33421 cggcatattg gaacggtagg tgtcgggtgt attgtcgatg tcgatggaaa cgacggattc
    33481 cgatgccgtc tgaagggttt tgccgttctt ggcgacaagg atgctgcgtc ctgcgttgac
    33541 aaggttgccg ttcaggttgt tgaggcgttt gatgtcggca atgctcatgc cggttgccgt
    33601 cgagatgtcg gacaggctgg ttttggcggc aggcgtatag acttcccatg aaaacaggct
    33661 gtcgggtgcg gcgttgaggt agttgctttg gaaggtttgt acggacgcga caggaagcag
    33721 cagtttgcgt ttgcttttgg ggataaacgc ggggacgttg aatgcggggt ttagggcgag
    33781 cagctcgctt tgcgtgatgc cggcaagccg ggcgatggct tcgttgtcga gcggacgatc
    33841 cggttcgact gcctgaaaat agggtttgtt gtctatgtcg ctgatattca tgccgaaaga
    33901 ttggggagtg gcaataatgt tgcgcacggc gagcagcttg gggacatagt tgcgcgtttc
    33961 gttgggcata cgcaggtttt cgtaggtcgg ttcgagccct tgggcgcggg cgcggttgat
    34021 ggcgcgtccg acgttgcctt caccccagtt gtaggcggca aaggcaagcg gccagtcgcc
    34081 gaacagtcca tagaggtatt gcagatagtt gagtgcggca tcggtggcgg cgtaaacgtc
    34141 gtgcctgccg tcgtaaaccg gtgttttttc caggccgtaa tgcctgccgg tagcgggcat
    34201 gaactgccac aggcccgatg cgccgacgtg tgatttggct ttggtgacga acgcgctttc
    34261 gatgaaggga agcagggcgg cttcggcggg catattgcgt tttttgactt cgttggcgat
    34321 atggtacata tagggtctac tccggttgat gaccctgttg aaatagctgt ggcttgcgat
    34381 gaatttgctt tcgtggcggc gtaccagttc gggattgact tcgcccatcc ggaagccttg
    34441 gcgcagctcg ctccacaggc tgccggattg gaaatattgt tttgtcgggg gcaggtcgag
    34501 tattgaagag tttaagcgca taatcgccaa accgatttgg tgtgatgagg tgttttgggc
    34561 gtataggaaa cccggacaaa cggacagacc tgacgcggtc agggcgatgg ttttgagttt
    34621 ggacatgatt tatatcggcg gaataaacat attttatcgt ccgatggtac tgtttgactg
    34681 aaaaagcgtc aagttttaca ggaactttat gcggattcgg aaatgggcgg ccgatgtgtt
    34741 ttttgacaga aatcctgttt ggaatagaat atcggcaggc aatctgtttg aaacggacgc
    34801 aatatggatg catggtttga agatacggcg atggggcggt atgttgcaaa attggaacag
    34861 gatttcttcg ggcggtatct agattcatac cgtttttcgg gaatgtgtgc ggttcaggtg
    34921 ggcggtccgt ggctgagcct gtctgaagat gttgtctgtg tgccgcgaga tatgtcgatg
    34981 tcggcggaga atatggcttt ggcggatgtt tctgcggata tgctgctttt tccgcatacg
    35041 cttgaaggcg gtatcccttc gcaaatcctg tcggaagcgc accggatatt gaaaccgtgc
    35101 ggacgcttga tgctgacggg tttcaatccg tattcgctct ggggattcag ccgttggttt
    35161 gacggcgaac gcctgccgga aaaacggttt tgtctgccgc tgcccgagtt gaaaaggcag
    35221 cttgcggatg tcggtttcga tattgaattt gggaaattta tggtgtatct gccgcccgtt
    35281 tcgtcgatcg ggcaaatacg cttttggcgg tttatggaaa aggcgggcga ccgttggtgg
    35341 ccgcaatgtg ccgcagtgta cgctttggtt ttggtcaaaa gggcggcggg cgtaacgccc
    35401 ctgcccgcgt gggacgggta tttgggcggc aaagcccttg cggcgggtgc ggcaagggtt
    35461 gcggattagg gacagccgtc tttcagacgg catctgtcag aaaaaatgtc gggaggggtt
    35521 atgccggaat tgccggaagt ggaaacgacg ttgcgcggca tcgcgccgca tattgaaggg
    35581 aaaacggtgg aagccgtggt attgcgccaa ttgaagctgc gctggcagat caatcccgat
    35641 ttgggggaga ttttgtccgg tcggcaggtt ttgtcctgcg gcaggcgtgc caaatatctg
    35701 attgtccgtt tccaaacggg gattttgctg attcacttgg ggatgtcggg cagcttgcgg
    35761 atttttacgc cgtcggacgg acgtatcggc aggccggaca gacacgatca cgtcgatatt
    35821 gtgttttcag acggcacggt catgcgttac cgcgatccga gaaagttcgg cgcgatactt
    35881 tggtatgagg gaatcgaaga acatcatccg ctgttggaaa aactggggcc ggagcctttg
    35941 tcggaggcat tttgtgcgga ttatctgtat gcaaggctga aggcgcagaa gcgcgcggtc
    36001 aaacttgccc tgatggacaa tgcggtcgtg gtcggtgtgg gcaacattta tgccaacgag
    36061 agcctgttta gggcgggcat ttcgccccac cgtcctgcca accgcctgaa aaagaaagag
    36121 tgcgcgcttt tggttgaaac cgtcaaagcg gtgttgcagc gcgccattga aacgggcggc
    36181 agtacgctga gggactttgt ggacagcgac ggcaaaagcg gctacttcca acaggaatat
    36241 acggtgtacg ggcggcacaa tcagccgtgc ccccagtgcg gcggtttggt tgtgaaagaa
    36301 actttggggc agcgcggcac gttttactgc ccgaattgtc agaaataggg cggaaaacgg
    36361 tttcagacgg cattttatcg gtatgccgtc cgaacgtttc aacaacaaac accgattatc
    36421 gggaaagaat tgctcatgtc ttcaaataaa gcttcatttt ttacacgtct gcgccgcttg
    36481 tgccgcttga cggtctggct gttcaaaacc gggaaaaacc tgcgcggtat tgacggcggt
    36541 tgccccgagt cgcgcaatcg ggcggtaatc gagttgggca ggggggtttt ggcggctttg
    36601 gatatcggat tggaggtggg cagacccgca cccgaacatc cgaacggtgt cttggttgcc
    36661 gccaaccacg tgtcctggct ggatattttc gcgatgagcg cggtttatcc gagcagcttt
    36721 atcgccaagc aggaaatcaa aagctggccg gtattgggca agatggggca gaacgcggga
    36781 acggtgttca tcaaccgcaa ttcgcggcgc gacatcgaac cgattaaccg cgccgtctgc
    36841 gaaaccttgc aacgcggtca aaacgtcagt tttttccccg aagcgcggac ttcctccgga
    36901 ttggggcttt tgccgttcaa agccgcgctg ttccaatccg ccatcgatgc gggggcaaag
    36961 gttttggcgg tcgcgctgcg ttattatgac gaaacgggaa aaaggacggc tcgtccctca
    37021 tatgccgatg tcggtttgcc gacctgcctg tggcgcatcg tgtctatgaa aaaattgacg
    37081 ataaaagtcg atttcgtttg cgtggcggat gcggcggaaa gcgaagaccg ttatgcttta
    37141 aaagataaaa tcgaagaaag catccgtgcc gttgtcgccg acgatgcgga tatcgctgtc
    37201 tgaaaccggt tgtcggaatg tggcagtatg attcgctttt gtggatgtat agtggattaa
    37261 atttaaacca gtacagcgtt gcctcgcctt agctcaaaga gaacgattct ctaaggtgct
    37321 gaagcaccaa gtgaatcggt ttcgtactat ctgtactgtc tgcggcttcg tcgccttgtc
    37381 ctgatttttg ttaatccact atattaaggc tgcggcaacg ggaggacctc aaacgaaatg
    37441 gcgtaaggat agtttgttgt attcataaaa tatgccgtct gaacctgtgt tcagacggca
    37501 tattttatat gggattaacg gcggaacctg ctgccgtcga aatcatcgaa agcctcataa
    37561 gccgcccggc attgggtttt ctgttcctta taaggcaggg agcggaatgc tttgcgcgcc
    37621 tttttgtttt cgcgcaattc ctgcacgttg ccttgcgatc ggtaccacgc ggcacggcgt
    37681 tccaaatatt tcctgcattc ggggtgcagc atctgcttgt ttgccttgcg gatatgtttg
    37741 gtatcgcggt gggcgggttc tgacgtaccg gcagcggcag ttgtcgatac cagtcccaat
    37801 attgcagaca acagtatggc aagcatcttt ttgacagata tgacgatcct ttcagtttgt
    37861 cattccttcc agtataggat ggaaggatgg aacgggcaaa ttgtcggcag tttcagactc
    37921 attcagccga caaaacttgc cgtttgatat gcggattcaa ataacacatt aatctcaata
    37981 ataaattcca atcggtaaga aaatggaatt tgtcggcggc ggcgcggcaa aatcatactt
    38041 tgcaaaattt aacaatttgc aggggcagaa aacaggaagc tttccttttt cgtcggaaaa
    38101 tccttatttc gccgccttgt agccggagcc ggtcaaaagg caaaaaattt acccgttttt
    38161 tatcggtaaa gaattatcag ataaaacaaa tattatagga aaaatacgac aggcgggttt
    38221 tatcgcgcat tgcctgaaac tgaaaaatac aaccgttgtc aagactggag aaaatgccaa
    38281 aaatccacta tattgtctgc cttaatttat ttgaaaagac tgtgtcttga atatcaagag
    38341 tggaagagga agcgatgaat acaccgactg atttgaaagt aaccaaacga gacggaagat
    38401 tagaagccat tgatttggat aagattcacc gtgtcgtcac ttgggcggcg gacggattgg
    38461 aaaatgtttc cgtatctcag gtcgagttga aatcgcacat ccagttctac aacggcatcc
    38521 gcaccgacga catccacgaa accatcatca aagccgctgc cgatttaatt tcggaagata
    38581 ccccggacta ccaatacctt gccgcgcgtt tggcgatttt ccatctgcgt aaaatagcct
    38641 acggcgagta cgagccgccg cacctttacg accacgttaa aaaacttacc gatgccgcaa
    38701 aatacgacag gcatatcctt gaggattaca gccgcgaaga atttgacgag ctgaacgcct
    38761 atatcgacca cgaacgcgat atgtcctttt cctatgccgc tgtcaaacag ctcgaaggca
    38821 aatatctggt acagaaccgc gttacccgcc agatttatga aacaccgcag ttcttatatg
    38881 ttctggtggc gatgtgtctt ttcagcaaat atcccaaaga aacacgcctc gattacgtca
    38941 aacggtttta cgatgccgtt tccacattta aagtatcgct gccgaccccg attatgagcg
    39001 gcgtgcgtac gcctacgcgc cagttctcaa gctgcgtatt gatcgaatgc gacgacagcc
    39061 tggattccat caacgccacc accagcgcga ttgtgaaata cgtttcccag cgtgcgggca
    39121 tcggcatcaa tgccggacgt atccgcggac tggacagcga aatccggggc ggcgaagcac
    39181 ggcataccgg ctgcattccc ttctttaaaa tgtttcaggc ggcagtcaaa tcctgttcgc
    39241 aaggtggcgt gcgcggcggc gcggcaacct tgttctaccc cttgtggcat atcgaagccg
    39301 aaagcctgct ggtgttgaaa aacaaccggg gcgtggaaga caaccgtatc cgtcagcttg
    39361 attacggcgt gcaaatcaac cgcctgctgt acacccgcct gattaagggc ggcaacatta
    39421 cgctgttttc gcccaacgag gttccgggat tgtacgaagc gttttttgcc gaccaagacg
    39481 aatttgagcg gctctatacg aaatacgagc aagaccctga tatccgcaag cgcatcattc
    39541 cggctgccga cctgttttcc acgctgatgc aggagcgtgc cggaaccggg cgcatctaca
    39601 ttcaaaacgt cgatcactgc aatacgcaca gcccgttcga tccgcgcgtt gcgcctgttc
    39661 atcagtccaa cttgtgtatg gaaatcgcgc tgccgaccaa acctttggac aatatcaacg
    39721 acccgaacgg cgaaatcgcc ctgtgtaccc tgtctgcctt caacttgggg gcattaaaca
    39781 gcttggacga attggaaggg cttgccgatt tgaccgtgcg cgcgctcgat gcacttttag
    39841 attatcaggg atatccggta gaagccgcgc gtacctccac tatgggccgc cgttcgctcg
    39901 gtatcggcgt gatcaactat gcgtattatt tggcgaaaaa cggtgtccgc tacagcgacg
    39961 gctccgcgct cggtctgacc caccgtacct ttgaagccat acagtattac ctgctcaaag
    40021 catcggcaaa ccttgccaaa gaatacggtg cgtgcacgct ctttaaccaa accgtttatt
    40081 cgcaaggcaa actgcccatc gacacctaca aaaaagactt ggacgctgtg tgtaacgagc
    40141 ctttgcacta cgattgggaa agcctgcgtg ccgacatcgt caaatacggc ctgcgtaact
    40201 ctactctaac cgcactcatg ccgtctgaaa ccagttcgca aatcgccaac gccaccaacg
    40261 gtatcgagcc gccgcgcgga ttggtaacgg tcaaagcatc gaaagacggc attttgaaac
    40321 aagtcgtgcc ggagtttgaa accctgaaaa atgcctatga aaccctgtgg cagcttcccg
    40381 gcaacgaagg ctatctgaaa cttgttggcg tgatgcaaaa attcgtcgat caggcgattt
    40441 ccgccaacac cgcctacgac ccgggcaaat tcgaaggcgg caaagtttct atgaaacaaa
    40501 tgctcaaaga cctgctgacc gcctacaaat acggcgtcaa aaccctgtac taccataaca
    40561 cccgcgacgg cgcggacgat acgcagaccg atattcagga tgacggctgc gcgggcgggg
    40621 cttgtaagat ttgatgaaag ggggagtttt cagatggcct ttagattaat aaatcatctg
    40681 aaatataaaa tatgaaaaat aaaatacaaa atcaattaga taatatagtt accttaaata
    40741 atgagaaatt tacttttatt gatttatttg cgggaatagg tggttttcgc attgcaatgg
    40801 agaatgttgg tgggcgatgt gtattttcta gtgaatggga tgataaagcc cgtcaaacct
    40861 accaagtaaa ttttaatgat attccttatg gagatattac attaaaggaa accaaagcag
    40921 ctattccaag taagtttgat gtattaacag caggattccc gtgtcagcca ttctctatag
    40981 ctggtgtttc aaagaaaaaa agcctaggac gagaaacagg cttcttagat aaggcgcagg
    41041 gaactctatt ttttgatgtt gctgaaatta ttggaaaaca tcgacctaaa atttttcttt
    41101 tagagaatgt gaaaaacctt gtttcgcatg acaaaggaaa tacatttaaa gtaattaaag
    41161 ggactttaga agagcttgac tatcagatat tttatcaagt tatgaatgca aaatattatg
    41221 ttcctcaaaa tagggagcgt atttttattg taggttttga tagacaatat tttaataagg
    41281 aaataaattt caattttcct tccccaccag aatcacaacc aaaattaaag caaattttgg
    41341 aagatgatgt agataattct tttactcttt ctgataactt atggctttac cttcaaaatt
    41401 acgctaaaaa acataaggca aagggtaatg gatttggttt tggattagtt gatttagatg
    41461 gaatatcacg aactctatct gcacgatatt acaaagatgg ttccgaaata ctcatccctc
    41521 agaaaggaaa aaatcctagg aagctgacac ctagggagtg ctcgcgttta atgggatttc
    41581 ctaaagattt tgttattgat gcagtatcca agacagctgc atacaagcag tttggcaatt
    41641 caattgctgt accgttggtt caagctattg ctaaacaaat tataaatgag ttaaaaaatg
    41701 aatgacttgg cagctcagac aatacaagta gtaaataaat ccgatgactt agtagcttca
    41761 gcaatacaga cagcaaataa atcaattgca gtatactgtc gttatattcg tcccaatgat
    41821 gttggtacta ctggtagtca ccaatcagga ttttatattc aaaaacattt ttcagacgac
    41881 ctctttgatg tagtttgcca aaagggaaca aataaaacta tttcaatcaa aattaattgg
    41941 caagatggaa gcgtcactaa tagcaatttt aaatattacg gccaaggcac gagaaatgag
    42001 gcgagaatta cgggttttgg caagaatttt gaatttttaa gtgataaata tagtggttcc
    42061 ttattggtat tgtgcagagc ttgttataaa gatttattat tccatgcttt tgttttatct
    42121 tcagatgaag atattgaaat atttatcgca gaaactaaca ttttaccagg aagtttatat
    42181 ttacctaaaa aacaagaagt agaggataac ctaacaaaat tattttcact atttcctaac
    42241 ttccccaaaa ctgaagaaat ggcagttttg gcaagggaaa atattgcatt aaataaaact
    42301 aacgttagta atgtgttaaa acaatgggtt tcaaaggaat atgaattatt ttccattttt
    42361 gaaaaacgtg aatttgaaat tattaaatca aaaattactg atttggatag cttcattaat
    42421 tttgcacatt cttttacaaa tcgcagaaaa gcaagagcag gaaaatcttt agagttacat
    42481 ttatcaagaa tatttgacga attttcctta aaatttgaga cgcaagcgaa gacagaaggg
    42541 aaaaagaaac cagatttttt atttcctgga agtgaagagt accatgcgat ggatgaatca
    42601 gggaatttca tatttcaaac tgaaaagctg acaatgctag gttcaaagac tacttgtaag
    42661 gatcgctggc gccaagtttt aaatgaagca gatcgaattc ctcataagca tttatttact
    42721 ctacaagaag gtatatctga tactcaaatt caggaaatga gtgatgagaa tttaacctta
    42781 gttgtcccta aagaatccgt aaaaacattt ggaacttttg ggaaaaccca tgttttaact
    42841 ttagaaaatt ttattaaata tataaagtct cagcaggtta gttaaaactt acagatcatt
    42901 tttaattaat atagagaata gattatgtcc tgcgaacact taactatgtc atacagcacc
    42961 tttcccaaaa ccaaaaacga cgcgctgaat gagccgatgt ttttcggtca gccggttaat
    43021 gtcgcccgtt atgaccagca gaaatacgag gtatttgaaa aactgattga aaaacaattg
    43081 tcgttttttt ggcggccgga agaaatcgac gtgtcgcgcg accgtatcga ctacgccaac
    43141 ctgccagaac acgaaaaaca tattttcatc agcaatctga aataccaaac actgctcgat
    43201 tccatccaag ggcgcagtcc gaatgttgcc ttgctgcctt tggtttcgat tcccgagttg
    43261 gaaacgtgga ttgaaacgtg gagcttcagc gaaaccatac actcgcgcag ctatacccac
    43321 atcatccgca atattgtgaa tgatccgtcg gtcgtgtttg atgatattgt cgaaaacgaa
    43381 tacattaccg cccgcgccga agacattgcc tgctattacg acgacttaat cgaatacacc
    43441 cagtattaca atctgttggg cgaaggggtg cacaatgtcg gcggaaaacc cattactgtg
    43501 tctttgcgcg ggttgaagaa aaaactctat ctctgcctga tgtgcgtcaa cgtgttggaa
    43561 gccatccgtt tctacgtttc attcgcctgc tcgtttgctt ttgccgagcg cgagttgatg
    43621 gaaggcaacg ccaaaatcat caaactgatt gcccgcgacg aagccctgca cctgaccggc
    43681 acgcagcata tgcttaatct gatgcgttcg ggtgtcgatg attctgaaat ggcagaaatt
    43741 gccgctgagt tgcaggacga atgtttccaa ctcttcaaaa aagcggcgga acaggaaaaa
    43801 gaatgggcgg catatttgtt taaagacggt tcgatgattg gtttgaacaa agaaatctta
    43861 tcccagtacg tcgaatatat taccaatctg cgtatgcagg cggtggggtt gccggccgga
    43921 tttgaaggcg caaatcaaaa cccgattccg tggattaatg cgtggctgtc gtccgataac
    43981 gtacaggtcg cgccgcagga agtggaaata tcctcttatt tgatcggtca gatcgattct
    44041 gaagtgaata cggatgattt gggcgatttt gagttgtaaa cgttttttga aaaaatgccg
    44101 tctgaagccg gatgtttcag acggcatttg tatgttgtat gatgggaaaa acagcgggta
    44161 ggatcggtta ttgttccttt tatagtggat tatagtggat taacaaaaat ccggacaagg
    44221 cgaggcaacg ccgtactggt ttaaatttaa tccactatat ttttgattgg tcggaaatgg
    44281 cgcgcattgg tacgaataag ggtttttttg agctcttgga gggcgagacc ctgttggaag
    44341 gcttggaacg gacggggcat atggtggaat atcagtgccg aagcggatat tgcggatcgt
    44401 gccgagtgaa gattttggag ggaagcgtta cttatcggga gccgcctttg gcttttttgg
    44461 ggcgggacga gatactgccg tgctgctgtt gcgtcgaggg ggatgtcagg ctggattgcg
    44521 gtttgtcggg ggaggatgag gggctgtctg acagtttgtt gaaataaaag ccgtccgaac
    44581 attggttcgg acggcttttt gacggtatta ccggttatgc ctgatgagtc ggtaaaaggt
    44641 ttcaccgtct tggatataac ccagttctac ccgggcgatt tccgaaatgg cttcttgacc
    44701 gttttccaaa tcatagactt cggcggcaag ggaatgattg cgtagggcga gtgtctggtt
    44761 tttttcttct tgaacggcaa tctgttctct cagactgctg ttgcgtccga tgctgccttt
    44821 gccgaaccag aggctgtatt ggcaacagac aagtgcgaag gataaaacga cagttaccca
    44881 cttcatacct taaccttttt tatttgccca gttggtagaa tgcggctttg ctggggtagt
    44941 cggcggcttc cgccaattct tcctcgatac gcagcagttg gttgtatttc gccatgcggt
    45001 cggaacggct caaagaaccg gttttgatct gcatacagtt ggtggcgact gccaagtcgg
    45061 caatggtgct gtcttcggtt tcgccggagc ggtggctcat tacgctggcg tagcggttgc
    45121 gtttggctaa gtcgacggct ttcagggttt cactcaaagt accgatttgg ttgactttga
    45181 ccaatagtgc gtttgccacg cctttttcaa tgccttcggc aaggattttc gggttggtaa
    45241 cgaagaggtc gtcgccaacg agttggactt tgccgcccag tttttcggtc agcagtttcc
    45301 agccttccca gtcgttttca tccatcccgt cttcgatgga gatgatgggg aactcgttga
    45361 ccaggccttc cagatattcg gcaaattccg cgttggtgta ggagcggcct tcggcttcca
    45421 agtggtattt gccgtctttg tagaactcgc tggacgcgca gtccaatgcg aataatacgt
    45481 cttcgcccgc tttgtagccg gcggcttcgg tcgcctcgac catcagttgc agggcttctt
    45541 tgtggctgtt caggttgggg gcgaaaccgc cttcgtcgcc gactgtggtc gggaagcctt
    45601 tgctgtcgca cagttttttc aaggcgtgga aaatttccgc accgcagcgc aacgcttcgc
    45661 ggaaagattt tgcgccgacg ggcataatca taaactcttg gatgttcagg ctgttgttgg
    45721 cgtgttcgcc gccgttgatg acgttcatca tcggtacggg cagggacatc gggcctgcgc
    45781 cgcccaagta gcggtaaagc ggcaggcctg agtcttcggc agcggcgcgt gcaaccgcca
    45841 tagaaaccgc caaagtcgca ttcgcaccca aattgccttt gttttcagta ccgtccaatt
    45901 cgatcatgat ttggtcgata taagattgct cgttggcatc gataccaatg agggcttggg
    45961 cgatttggtt gttgacgtgt tcgaccgcct tcaatacgcc cttgcccgaa taacgggatt
    46021 tgtcgccgtc gcgaagttcc aaagcctctt tttgaccggt ggacgcgccg ctcggtacgg
    46081 ctgcgcgtcc cattacgccg gattcgagca atacatcaca ctcgactgtg gggttgccgc
    46141 gtgagtccaa aatttcgcgg gcgaaaatat caacgattgc gctcatgaat gttctccaag
    46201 tgaagtgaag ggaggtaaaa aatgggaggg cttcagacgg catctttgcc ggaaagccgc
    46261 caacttaaaa ctgctgaaag tagggcggtt gccataaaaa caatactgga aatgccgtgc
    46321 cacattccga aggatccgcc tacaaacgac aacagccaat tttcatgtcc gtatttcagt
    46381 gcctcgataa cgggtgtaat caagaatcgg ttggcggcaa gggcggacaa taaaaaaagc
    46441 agcagggcgg tttgccgccg ggttagggcg gcaaaggcgg cagccagtac cgcgccccaa
    46501 acggcaagcc cgctccaaga gaggatgtcg aacaatacgc cggcaatttc gcccgcctgc
    46561 attttgggca gcattttgaa cagcaccggc gcgacgatat aacccgccat aatctgcata
    46621 ccgagccaca aggttgcggc ataccggctg atttttcgga aagtctgcat cataaggcga
    46681 aaccgtgtta ctcgattgtt aaaatcggtt gtgatttgat taaatcgtcc aatgctttga
    46741 tgcggattaa aaaatcttct aaaaggtgca gcggcagcgc gctggggccg tcgcattttg
    46801 ccagtttcgg atcggggtgc gattcgagga acagaccggc aaggcgggtt gccatgcctg
    46861 caagtgccaa atccaaagcc tgtgcgcgac gaccgccgga tgcggcagaa ccagcatcgc
    46921 gggtttgcag ggaatgggta acgtcgaaaa taaccggcag gttgccgcag gtttgtttca
    46981 tcacgcagaa accgagcata tcgacaacga ggttgtcgta gccgaagctg ctgccgcgtt
    47041 cgcataaaat cagtttcccg ttgccggctt cgtggaattt ttccacaatg tttttcattt
    47101 gagaggggct gaggaactga ggttttttga tgttgacgac gttgccagtt tttgccatgg
    47161 caaccactaa atcggtctgc cgcgcaagaa aggcgggaag ctggatgaca tcgcacactt
    47221 cggcgacggg ttggcactga tggggttcgt gtacgtcggt aatgacgggg atgccgaact
    47281 ctgctttgac tttttcaaaa atctttaagc cttcttccaa gcctacgccg cgataagaat
    47341 ggatggagga acggtttgcc ttgtcgaaag aggctttaaa gatataggga attccgagtt
    47401 tgcgggttac ttcgacgtaa tgcgcgcagg tttggagggt ggaatccaag ctttccaaaa
    47461 cgttgatgcc gccgaatagg acgaagggcg agttgttgcc gatggtgatg tcgttgattt
    47521 taatatccat aatcgatgtc cgtttgcgga attacagcac ggtgtgcggc ggctcgtagg
    47581 agatgatgtc gcggattttg ttgttcccgt ccactaagac gactttgggt tcatgcgcgg
    47641 cgatttcggg ttcggagagt tggacgtaag acatgatgat gacgatatcg cctttctgca
    47701 ccagccttgc cgcagcaccg ttcagacaaa tcacgccgct gccgcgttta cctgaaatgg
    47761 tataggtttc aaaacgttcg ccgttgttgt tgttgacgat ggcgactttt tcgttggggt
    47821 agatgcctgc cgcgtctaac agatcttgat cgacggtaat gctgccgacg tagttcaaat
    47881 cggcttcggt tacggtggcg cggtggattt ttccgccaag catagtacgg aacatgtatt
    47941 tccttttggt ttgtgtgcgg tctgacgggc gcgatgccgt ctggaaatct gaatgccgta
    48001 ttatagtgga gtttccttca aattcataat ggcggctttc ggacaaggaa aacggtgttt
    48061 cagacggcat tgcggtgtcg gctttatttg ggcaggcatc tgttttgcaa ggtttttaaa
    48121 acgtttttta cggtgttgat tctgacctcg atattttcca tctctgcggt aaaccgcagc
    48181 ttatcggcgc cggcaaggcg gtattttttg tcgttctgaa tcagcaggat gatttcggtt
    48241 ggatcgacat tgtggttttt gccgaaggtt accgttaccg attcgccggc cgcatcgatg
    48301 gcatcaatac ccaattcttt tgccgcaagc cgtaagtggt ggctttcgat gagggttttg
    48361 acgggttgtt cgggcaggcc gaagcggtcg acgagttctt cgtgtatggc gttgatttgc
    48421 tgcacggttt cgcagacggc gaggcgtttg tagaggacga gacgctcgtg gatgtcgggg
    48481 cagtagcttt cgggcagcag agcggggctg tgcagtttga tttcggtggt aatgcccaat
    48541 ggcgcgtcga ggtcgggctg gcggcctttt ttgaggtcgc gcacggcttg tttgagcatt
    48601 tcggtgtaga gcgtgaagcc gacctgtatc atttcgccgg actgcccttc gccgaggatt
    48661 tcgcctgcgc cgcggatttc caaatcctgc atggcgaggg tgaaacctgc gccgagttcg
    48721 tctgctgccg cgatggcgtc gaggcgtttt tctgcgtctt tggtgatgta ttcgggcgtg
    48781 agcaggtagg cgtaggcttg gtgatggctg cggccgacgc gcccgcgaag ttggtgcagt
    48841 tgcgccagcc cgaatttgtc ggcgcggttg atgatgatgg tgttggcgtt ggggatgtcg
    48901 atgccggttt cgatgatggt ggaacagagc aacacgttga atcgttgctg taaaaagtcg
    48961 cgcatgactt gttccaactc gcgctcgcgc agttgtccgt gcgctacgcc gatgcgggct
    49021 tcgggcagca gggtttccag ccgctcgcgc atattttcaa tcgtatctac ttcattgtgc
    49081 aggaaaaata cttgcccgcc gcgtttgagt tcgcgcaaca cggcttcgcg cacgctgcct
    49141 tcgctgaagg gtttgacaaa ggttttgacg gcgaggcggc ggctcggtgc agtggtaatc
    49201 agcgagaagt cgcgcagacc ttccaacgcc atacttaaag tacgcggaat cggcgtggcg
    49261 gtcatggtga ggatatcgac attggcgcgc aggcgtttga gctgctcttt ctgtcgcacg
    49321 ccgaagcggt gttcttcgtc gataatcact aaacctaagt ttttgaattt tatgtcgtcc
    49381 tgcaccaatt tgtgcgtgcc gataatgata tcgaccgtgc cgtccgccat gccttcgagc
    49441 gcggctttgg tggctttgct gttgttgaaa cgcgaaaggc cggcgacttt cacgggaaaa
    49501 tcggcgaaac ggtcggcaaa gttttgcgcg tgctgctcga ccagaagcgt ggttggggcg
    49561 agtacggcga cctgtttgcc acccatcacc gccacaaacg cggcgcgcag ggcgacttcg
    49621 gttttgccga aaccgacatc gccgcacaca aggcggtcca tcggcttcgc ctgcgtcaag
    49681 tctttaatca cggcggcaat ggcggctgcc tggtcttcgg tttcctcgta gccgaagccg
    49741 tcggcaaacg cctgataatc caactcgttg atttcaaact tgtgtcccga ttgggcggcg
    49801 cgttgggcat agagattgag caactcggcg gcggtatcgc gcgctttttc ggcagccttg
    49861 cgttttgcct tgttccacgc gccgctgccg agcttgtgca gggcaatgtt ttcatgcgcc
    49921 tgaccggagt agcggctgat taaatgcagt tgcgaaacag ggacataaag ctgcgcttcg
    49981 cctgcgtatt cgagcaacat catttcgttg gtttcgccgc ccaagtccat cgttaccaag
    50041 cccatatacc gcccgatgcc gtgttcttcg tgcacgacgg gatcgccgat gttgatttcg
    50101 gcaaggtcgc gcaacaggcc atctgaaacg gcggcgtgtt tcttgcgatg atatttgcgc
    50161 gagcgggcaa catattgata caaatcggat tcggtaatga cagcgatggc t
//
LOCUS       DAWFLT010000004        48967 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWFLT010000004 DAWFLT010000000
VERSION     DAWFLT010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 48967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48967
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.004"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(73..3678)
                     /gene="putA"
                     /locus_tag="WGR74_000209"
     CDS             complement(73..3678)
                     /gene="putA"
                     /locus_tag="WGR74_000209"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ0904713.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDKNISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADKLDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTELAASGIQAVVEPDSPLASYTADLEGLLLVNDKPETAGISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
     gene            3670..3837
                     /locus_tag="WGR74_000210"
     CDS             3670..3837
                     /locus_tag="WGR74_000210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904714.1"
                     /translation="MKHFLDSPKFFGGQAASVRPEYGNIVINPQMKYKASNAGRVGAS
                     VSLKMKRVKHK"
     gene            complement(3927..5453)
                     /gene="putP"
                     /locus_tag="WGR74_000211"
     CDS             complement(3927..5453)
                     /gene="putP"
                     /locus_tag="WGR74_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ0904715.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     NVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            5936..6256
                     /locus_tag="WGR74_000212"
     CDS             5936..6256
                     /locus_tag="WGR74_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ0904716.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            6270..7766
                     /locus_tag="WGR74_000213"
     CDS             6270..7766
                     /locus_tag="WGR74_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ0904717.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPIKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            7872..8717
                     /gene="ftsN"
                     /locus_tag="WGR74_000214"
     CDS             7872..8717
                     /gene="ftsN"
                     /locus_tag="WGR74_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0904718.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPV
                     PSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAATEAEQSDAEKAADKADEVEEK
                     AGEPEREEPDGQAVRKKVLTEEREQTVREKAQKKDAETVKKQAVKPSKETEKKAPKEE
                     KKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTSDKAEATHYLQMGAYADRR
                     SAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHEVASLIR
                     SIESK"
     gene            8720..9364
                     /locus_tag="WGR74_000215"
     CDS             8720..9364
                     /locus_tag="WGR74_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0904719.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQLEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYRIDSTPTVVVGGKYRVIFNNGFDGGVHTIKELVAKVRAERKLSNHPVQK"
     gene            9398..10219
                     /locus_tag="WGR74_000216"
     CDS             9398..10219
                     /locus_tag="WGR74_000216"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ0904720.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLVGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGLGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGIAQVFA
                     LIPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(10510..10585)
                     /locus_tag="WGR74_000217"
     tRNA            complement(10510..10585)
                     /locus_tag="WGR74_000217"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10550..10552),aa:Ala,seq:ggc)
     gene            complement(10593..10669)
                     /locus_tag="WGR74_000218"
     tRNA            complement(10593..10669)
                     /locus_tag="WGR74_000218"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10633..10635),aa:Met,seq:cat)
     gene            complement(10801..12018)
                     /locus_tag="WGR74_000219"
     CDS             complement(10801..12018)
                     /locus_tag="WGR74_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0904721.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQSSLKLQTLI
                     AEKIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLEHGCMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYL
                     PQSHFLLNLGITELLAQTGKTNSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGID
                     WAGFRFGDICHKL"
     gene            12297..12752
                     /gene="mraZ"
                     /locus_tag="WGR74_000220"
     CDS             12297..12752
                     /gene="mraZ"
                     /locus_tag="WGR74_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ0904722.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLGQTDLQL"
     gene            12749..13735
                     /gene="rsmH"
                     /locus_tag="WGR74_000221"
     CDS             12749..13735
                     /gene="rsmH"
                     /locus_tag="WGR74_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ0904723.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            13732..13995
                     /gene="ftsL"
                     /locus_tag="WGR74_000222"
     CDS             13732..13995
                     /gene="ftsL"
                     /locus_tag="WGR74_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ0904724.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            14056..15801
                     /locus_tag="WGR74_000223"
     CDS             14056..15801
                     /locus_tag="WGR74_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ0904725.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            15826..17304
                     /locus_tag="WGR74_000224"
     CDS             15826..17304
                     /locus_tag="WGR74_000224"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ0904726.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKFWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELVGRLKKDCPDLAVYSYGFSEHADIRIIDF
                     TASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            17327..18972
                     /locus_tag="WGR74_000225"
                     /pseudo
     CDS             17327..18972
                     /locus_tag="WGR74_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            19097..20455
                     /locus_tag="WGR74_000226"
     CDS             19097..20455
                     /locus_tag="WGR74_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ0904727.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLTALQMLAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGNECAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAVDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            20458..20634
                     /locus_tag="WGR74_000227"
     CDS             20458..20634
                     /locus_tag="WGR74_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904728.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVSAR"
     gene            20728..21810
                     /locus_tag="WGR74_000228"
     CDS             20728..21810
                     /locus_tag="WGR74_000228"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ0904729.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            21925..22197
                     /locus_tag="WGR74_000229"
     CDS             21925..22197
                     /locus_tag="WGR74_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ0904730.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     NNLGVMYAERRGVRQDRALAQEWLGKACQNGYQDSCDNDQRLKAGY"
     gene            22281..23618
                     /locus_tag="WGR74_000230"
     CDS             22281..23618
                     /locus_tag="WGR74_000230"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ0904731.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAELQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKDKIFRGDGVQV
                     LNADDTFCCAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATQDIPLQGLHN
                     AANVMAAVALCEAVGLPRNELLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRHDLDGCGLN
                     MTDCATLEEAVQKAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIGAFKAL"
     gene            23744..25069
                     /locus_tag="WGR74_000231"
     CDS             23744..25069
                     /locus_tag="WGR74_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ0904732.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            25073..26140
                     /gene="murG"
                     /locus_tag="WGR74_000232"
     CDS             25073..26140
                     /gene="murG"
                     /locus_tag="WGR74_000232"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ0904733.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADIAGLPAPDERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRNKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            26295..27704
                     /locus_tag="WGR74_000233"
     CDS             26295..27704
                     /locus_tag="WGR74_000233"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ0904734.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFVDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLSAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            27817..28731
                     /locus_tag="WGR74_000234"
     CDS             27817..28731
                     /locus_tag="WGR74_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ0904735.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKSRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPSIHIIPATEFYDYEAKYNRNDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            28721..29449
                     /locus_tag="WGR74_000235"
     CDS             28721..29449
                     /locus_tag="WGR74_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ0904736.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYAYDGLPEKESEE"
     gene            29535..30779
                     /gene="ftsA"
                     /locus_tag="WGR74_000236"
     CDS             29535..30779
                     /gene="ftsA"
                     /locus_tag="WGR74_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ0904737.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVTSCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            30898..32076
                     /gene="ftsZ"
                     /locus_tag="WGR74_000237"
     CDS             30898..32076
                     /gene="ftsZ"
                     /locus_tag="WGR74_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ0904738.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            32215..33525
                     /locus_tag="WGR74_000238"
     CDS             32215..33525
                     /locus_tag="WGR74_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ0904739.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            34151..35026
                     /gene="prpB"
                     /locus_tag="WGR74_000239"
     CDS             34151..35026
                     /gene="prpB"
                     /locus_tag="WGR74_000239"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ0904740.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            35112..36266
                     /gene="prpC"
                     /locus_tag="WGR74_000240"
     CDS             35112..36266
                     /gene="prpC"
                     /locus_tag="WGR74_000240"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ0904741.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            36418..37206
                     /locus_tag="WGR74_000241"
     CDS             36418..37206
                     /locus_tag="WGR74_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEZ0904742.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFVGGVAGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFMLHGSIIFPIAATMAAGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIVSMF"
     gene            37287..39893
                     /gene="acnD"
                     /locus_tag="WGR74_000242"
     CDS             37287..39893
                     /gene="acnD"
                     /locus_tag="WGR74_000242"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ0904743.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNRQIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVVLTGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATVDLAAKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRFGLKRKPWVKTSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRVHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSFARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPDTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            40696..41865
                     /gene="prpF"
                     /locus_tag="WGR74_000243"
     CDS             40696..41865
                     /gene="prpF"
                     /locus_tag="WGR74_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ0904744.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFSIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            42279..43478
                     /locus_tag="WGR74_000244"
     CDS             42279..43478
                     /locus_tag="WGR74_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0904745.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMDKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELVGIL"
     gene            43836..44456
                     /locus_tag="WGR74_000245"
     CDS             43836..44456
                     /locus_tag="WGR74_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ0904746.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKEVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRRGCGDTAVFGL"
     gene            complement(44583..45026)
                     /locus_tag="WGR74_000246"
     CDS             complement(44583..45026)
                     /locus_tag="WGR74_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ0904747.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLATPPDRINIGSVVRHLEPMQLVECMGSNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            45200..45568
                     /locus_tag="WGR74_000247"
     CDS             45200..45568
                     /locus_tag="WGR74_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0904748.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEETKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            45613..46767
                     /locus_tag="WGR74_000248"
     CDS             45613..46767
                     /locus_tag="WGR74_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ0904749.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVLAWLSAVFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(46824..47696)
                     /locus_tag="WGR74_000249"
     CDS             complement(46824..47696)
                     /locus_tag="WGR74_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ0904750.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVENLWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            47781..48593
                     /locus_tag="WGR74_000250"
     CDS             47781..48593
                     /locus_tag="WGR74_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ0904751.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTISAGREVPHLSAEGVSVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKYRVLDAV"
BASE COUNT        11256 a        12382 c        13905 g        11424 t
ORIGIN      
        1 ttataaaaat gccgtctgaa aggttttcag acggcattgg ttcacgggcc gcgcccgggg
       61 atttcggtaa aatcagtcgg caaccgccat caggctggcg ttgccgccgg cggctgtggt
      121 gttgacgctg caagagattt cttcaaacac ttgcaggatg tcgagtccgt tttccgaagg
      181 gaggatgcgg atgagtgcgc cgtcgtgggc ggcaagttcc tgtttgcgcg cgctgtccaa
      241 aggcgacagg gcggcaacgt ggctgatgcc ggcggtttcg ggtttgtcgt tgaccagcag
      301 cagaccttcc aagtcggcag tgtaggaagc caaggggctg tcgggttcga ccactgcctg
      361 tatgccggag gcggcaagtt cggtcagtgc ggcaaaggct tgaaccgtgc tgccgccgtg
      421 tatccaaacg cgtttgggcg cgtgccatga gatgctgttg cgctcgccgg tcggtccggt
      481 aaggacggtt tcggcacggc gcagggtgcg gatgcgggcg tgtcccaaag cggccgctgc
      541 ggcttttttc tcttcggcgt tgaacggcag tttgtgaacc agtgcttcga ggcgtttgag
      601 tgcggcttcg tctgcctgtc cgatttggct cagggtcggg gcaacccatt cggggatgcg
      661 ggtcagtttt tgcaggtaga acgaaccgcc tgctttgggg cctgtgccgg acagaccgtg
      721 tccgccgaag ggctgtacgc cgacgactgc gccgacgatg ttgcggttga cgtaaacgtt
      781 gccggcttcg atgcggctgc ggatgtggcg tactgtgcct tcgatgcggc tgtgtacgcc
      841 gtgggtcagg gcgtagcctt tgctgttgat ttggtcgatg acgctgtcga gtttgtcggc
      901 gcggtagcgg acgacgtgca ggacgggacc gaagacttcg cgttgcagtt cgttgaggtt
      961 gttcaattca aacaggatgg ggcggacgaa cgtggatttt tcggaatcga catcggcagc
     1021 ggttttgact tcgtggtaag acttggcaac gcctttcatt ttgttgatgt ggttcaacag
     1081 gttttgctgt gcttcggcat cgatgacggg gccgacatcg gtagtgagct gaatcggttt
     1141 gccgacgacg agttcgtcca tcgcgccttt gatcatgtcg agcatacggt cggcaacgtc
     1201 ttcttggacg cacaagatac gcagggcgga gcagcgttgt cccgcgctgt cgaaggcgga
     1261 gttcaatacg tcggcgcaga cttgttcggc aagtgcggtg gaatcgacaa tcatggcgtt
     1321 ttgtccgccg gtttcggcaa tcaggacggg attgtcgccg cgtttggcaa gggctttgtt
     1381 gatcaggcgc gccacttcgg tcgagccggt gaaaatcacg ccgccgatgc gggcatcgtt
     1441 ggtcaatgcc gcacccacgt cgcctgcgcc gaggacgagt tgcagggcgg aagtcgggat
     1501 gccggcttcg tgcatgaggg aaacggcata accggcaatc aggctggttt gttcggcggg
     1561 tttggcgatg acggtgttgc ctgccgccaa tgcggaaacg acttcgccgg taaagatggc
     1621 gagcgggaag ttccacgggc tgatggcgac aatcgcgccg acggcttttg cgtcttgagg
     1681 cagggtatgt tcggcttcgt ttgcgtagta gcggcagaaa tcgacggctt cgcgcacttc
     1741 ggcaatggcg ttgttcagcg ttttgcctgc ttcgcgcacg gcaagcatca tcagcgcggg
     1801 ggtgtgctgt tcgagcaggt cggcgaaacg gcgcaggcag gcggcgcgtt cggcggcagg
     1861 tgtcgcactc cattcgggga acgcggcaac ggctgcgcca accgcttctt gggcaagcgc
     1921 ggcatcggca aagctgactg tgccgacgat gtcgtcgtgg tcggcagggt ttttaatcgg
     1981 ttgcgcttcg ccgacatcgc gggctttgcc gttgacgatg gatgcggcgt ggaagtcttg
     2041 cgcggcggct ttgttcatct gttcttgaag ctgctgcaat acgttttcgt tgctcaagtc
     2101 cacgccttgc gagttcagac ggcatttgcc gtacaaatcg cgcggcagcg gcagggcgtt
     2161 gtgcaggtgg atgccttgtt cggcgatggt gtcgaacggg ctgcggatga gcgtgtcgat
     2221 gctgatgttt ttatcgacga tttggttgac gaaagacgag ttcgcgccgt tttccaacag
     2281 gcggcgcacc aagtaggcga gcagggtttc gtgtgtgccg accggggcgt acacgcgcac
     2341 gcggcggcct aagttttgcg ggccgacgac ttggtcgtac agggtttcgc ccatgccgtg
     2401 cagacattgg tgttcaaagt ctttgccttt gcccatttgg tagattgcgc ccaaggtgta
     2461 ggcgttgtgg gtggcgaatt gcggaaatac cgcgtcttgc gcggaaagca gtttgcgcgc
     2521 gcaggcgagg taggagatgt cggtgtggac tttgcgggtg taggtcggat agccgttcaa
     2581 gccgtccact tgcgcccatt tgatttcgct gtcccaatac gcgcctttga cgaggcggat
     2641 cattagtttt tggttgttgc ggcgggcaag gtcgatcagg tagtcgataa cgaacggaca
     2701 acgtttttgg taggcttgaa caacgaaacc gatacctttg tagcctgcca aatcgggatc
     2761 ggaaacgagt gcctccatca aatccaaaga cagctccaga cggttggctt cttcggcatc
     2821 gatgttgata ccgatatcgt attttttacc caaaaggaac agctctttca ggcgcggcaa
     2881 cagttcgccc atcacacggc cgtgttgggc gcgcgagtag cgcggatgga tggcggagag
     2941 tttgacggaa atgccgttgc cttcgtaaac gccttgtcct gccgcatctt tgccgatggc
     3001 gtggatggct tcgacatagt cgcggtagta gcggtcggca tcggcttggg tgtaggcggc
     3061 ttcgcccaac atatcgaagg agaagcggta gcccattttt tcgcgttctt tgccgttttg
     3121 cagggcttct tcaatggtct gtccggttac gaactgtttg cccaacaggc gcatggcgta
     3181 atttacgcct tggcggatga gcggtgcgcc gcctttgctg atcaggcggc tgagtgcgga
     3241 actcatttgt ctgtcgtttg tggcggtcag tttgccggta atcagcaggc cccaggcggc
     3301 ggcgttgacg aagagggaag ggctgttgtt caaatggctt ttccagttgc cgtctgaaat
     3361 cttgtcggca atcaggcggt cgcgcgtggc gttgtcgggg atacgcagca gggcttctgc
     3421 cagacacatc agcgcgatgc cttcttcgct ggagagtgaa aactcgtgca tcagcgcatc
     3481 tacgccgccg gctttggtgc ggccggcgcg gacttgggta accaaacggc gggcaagctc
     3541 ggaggcggcg ttgcgctctt cgtcgctcat ctgtgcacgt tgcaacatat cctgtacggc
     3601 ttcgatttca ttacggcggt aggcatcggt tatcgcttgg cgcagggcag tttgtgccgg
     3661 aaatgcaaaa tgaaacattt tttggattct ccaaagtttt tcggggggca ggcggcatcg
     3721 gtgcggcctg aatacggtaa tatcgtaata aatccgcaga tgaaatacaa ggcttcaaat
     3781 gcgggcaggg taggtgcttc cgtttctttg aaaatgaaac gggtaaaaca caaataaggc
     3841 ctgtatgcag gcaaggttta tttgtgtttg acccggaaac gggttcagac ggcacgaacc
     3901 gggatgccgt gccgtctgaa aggggtttat cgggcggcgc ggtaatctgc gtcggctttt
     3961 tcaaagcgtt cttgggtttc gcgcgaaggt tctttgttga acagggaaac caacacggca
     4021 acgatcaagc aaacaataaa gcccgggacg atttcgtaca tcgtcaacaa gccgctttcg
     4081 cctgccgctt gagccggttt tttcacccat tccgcccata cgactacggt taaggcacct
     4141 gcaaccatac ccgacaacgc gccgtaggca gtgatgcgtt tccacaatac ggacagaatc
     4201 acaatcgggc cgaatgccgc accgaaacct gcccacgcat aagacaccag tcccaatact
     4261 ttgctgttcg gatcggaagc aatcaggatg gaaatcacgg caatcgccaa gaccatcagg
     4321 cggccgaccc ataccagttc cgactgttgc gcgtttttac gcaaaaagcc tttgtagaag
     4381 tcttcggtaa tcgcactgga gcaaaccaaa agctggcagg acagggtgga catcaccgcc
     4441 gccaaaatcg cgctcaaaat aatgccggcg atccaagggt tgaacagcag ggtggaaagc
     4501 gcgatgaaga tgcgttcgtg gttgccgctc atagaagaaa ctttgtcggg atttgcaccg
     4561 aaatacgcaa tgccgaaata accgaccgct accgcgcccg caaggcacaa cgccatccaa
     4621 gtcataccga tgcggcgtgc ggataccagc gatttcgcgc tttcggccgc cataaagcgc
     4681 gccaaaatgt gcggctgtcc gaaatagccc aagccccagg cggcggtgga aatgatgccg
     4741 atgacggtcg taccggcaaa caggctgccg tattctttgc ccgtgcctgc ggcgacgttt
     4801 tgaatcgcgg cagacatctg ttccgcgccg cccaagccca gatagaccat cacaggcgtt
     4861 aaaatcagcg cgaaaatcat caaagaagcc tgcaacgtat ccgtccagct gaccgccaag
     4921 aagccgccca agaaggtata ggcgatggtc gcgcccgcgc ccagccacat tgcctgattg
     4981 taagtcatac cttcaaacag gctttggaac agggttgcgc ccgccacaat gcccgaggcg
     5041 caataaatcg tgaagaagaa caggataatc agtgcggaaa ccactttcat caagtgtccg
     5101 cccgcgccga agcggtggaa gaaataatcc ggcagcgtca gcgcattgtt ggcgtattcg
     5161 gtatgcacgc gcaggcggcc cgccaccaag agccagttga aatacgcgcc gaccaagagg
     5221 ccgatggcaa tccaagcctc attcaaaccg ctcaggtaaa tcgcgcccgg cagacccatc
     5281 agcagccagc cggacatatc ggacgcgcct gccgacatcg cggtaacaaa cgggcccagg
     5341 ctgcgcccgc ccaaaatgta atcgtcgaaa ttgcgcgtag aaaaataggc ggcaagcccg
     5401 atgagaagga ctgcaaccag atagattgca aaagtaatgt acatgggatt catgtgctat
     5461 tcctcgtcta aaacttcaga attacaggct ttgaaattgc aagcaacttg cgcctgaaat
     5521 gtttttctaa taaaagtaca acggaaaatc cggatacccg aaagggggat tcggataaat
     5581 tatcttcaat cacaataaga tatgtaataa aactatatga aattgtaaat aatccgtttc
     5641 agcataaccc aatttctgtt gtttgcaaag cacttaatgg cttaaaaagc cgagtttgaa
     5701 acgatgcgcg tcggaaaaat catttaaaac agcatattgt tttgtagtgt cttgtaatcg
     5761 ggcgttgcgc ggaatatgaa atccgttttc aggcggcagg tgttttgagg tgtaatttag
     5821 caaccgcaaa ggaggcgcgg tatgttttgc cgattatccg ccgcccgttt tcagacggca
     5881 tttttcctta tacaatagcc gattgaattt gatatgttca ggaaggatac agattatgtt
     5941 cggcaagcag ctttttgagg aagtcggctc gaaaatcagc gaaaccatcg ccaacagccc
     6001 tgccaaagat gtggaaaaaa atattaaggc gatgctgggc ggcgcgttca accgtatgga
     6061 tctggttacg cgcgaagaat tcgacatcca gcagcaggtt ttaatcaaaa cccgtaccaa
     6121 actggcggct ttggaagcgc gtttggcaaa actcgaagcc gcgcaaaatc ccgaacgggc
     6181 agcattggaa gcggctgaag ccgctgccga agaagccgtc gccgaaatca ggcagcaaac
     6241 cgaagccggc gaataaggcc gtctgaaata tgtcgcttgc cttggtttac agccgcgcct
     6301 tgagtggtat gaatgcgccg ttggtcgaag tggaagccca ccttgccaac ggcctgccgc
     6361 atttcaacat cgtcgggctg cccgatacgg aagtaaagga aagtcgcgac cgcgtccgtg
     6421 ccgccattat tcaaagcggt tttgaattcc ccgccaaaaa aatcaccgtc aacctcgccc
     6481 ccgccgacct gcccaaagag tcggggcgtt tcgatttgcc gattgcaatc ggcatccttg
     6541 ccgcatcggg gcaggttgcg cccgaaaaac tggcggaata cgagtttgcg ggggaattgg
     6601 cactgtcggg gctgttgcgc cccgtgcgcg gcgcgttggc gatggcgtgg cagggtatgc
     6661 aggcaaaacg tgcatttgtt ttgcctgaag aaaacgcagg acaagccgcc gtgatgcgcg
     6721 gcattaccgt ttacggcgcg cgctctttgg gtgaagtcgc cgcccatttg aacggcatcg
     6781 aacctttggc gcaaaccgaa tgttccgtac cgcaaatgcc gtctgaacat ggcggacaac
     6841 ctgatttgtg cgatgtgaaa ggtcagcaca ccgcgcgcct tgctttggaa atcgctgccg
     6901 caggcggaca cagcctcttg atgatgggtc cgccgggaac gggcaagtct atgctctccc
     6961 aacggctgcc cggcatcctg ccgccgctga ccgaagacga attggtagaa gtttgggcat
     7021 tgcgttcgct cctgcccaac caccaacaac aactcgacag caaccgtcct ttccgcagtc
     7081 cgcatcacag cgccagcgcg gcggctatgg tcggcggcgg ttcggatccg cgcccgggcg
     7141 aaatttcatt ggcgcaccac ggcgtattgt ttttggacga gctgcccgag ttcgaccgca
     7201 aagttttaga agttttgcgc gaaccgttgg aaaacggcga aatccacatt tcccgcgcgg
     7261 cgcgccaagc cgtctatcct gccaaattcc aacttgttgc cgccatgaac ccttgtcctt
     7321 gcggttatct cgggcatccc atcaaaccct gccgttgcac gcccgaaagc gtcgcgcgtt
     7381 accgcagcaa gatttccggc ccgctgctcg accgcatcga tttgaccatc gaagtcccga
     7441 gcctgtccgc cgccgaactg atgcagcagg aagcagggga aagcagcgcg tccgttttgg
     7501 aacgcgttat cgccgccaga gacaaacaat acgcacggca aggcaaagtg aatgccgctt
     7561 tgagcgtcag cgaactcgat tcccaagccc gcattcaaaa agaagcgcag gaagcattgg
     7621 gcggcctgct ggaaaaactc tccctttccg cccgcagctt ccaccgcatt atgcgcgtgg
     7681 cgcgtacatt ggcggatttg gcgggcgacg aagaagtcgg cagaagccac gtcatgaaag
     7741 ccataggttt ccgtcgcgct ttataggaac gggaatggaa gcaggttttg cccaaatatg
     7801 gcgatattgt tagaatatcc gcccgtaagc aaacggcgtt aatgccgtct gaaacacatt
     7861 aaggtatgtt tatgaacaaa ttttcccaat ccggaaaagg tctgtccggt tttttcttcg
     7921 gtttgatact ggcgacggtc attattgccg gtattttgtt ttatctgaac cagagcggtc
     7981 aaaatgcgtt caaaatcccg gttccgtcga agcagcctgc agaaacggaa atcctgaaac
     8041 cgaaaaacca gcctaaggaa gacatccaac ctgaaccggc cgatcaaaac gccttgtccg
     8101 aaccggatgc tgcgacagag gcagagcagt cggatgcgga aaaagctgcc gataaagccg
     8161 acgaggttga agaaaaggcg ggcgagccgg aacgggaaga gccggacgga caggcagtgc
     8221 gtaagaaagt gctgacggaa gagcgtgaac aaaccgtcag ggaaaaagcg cagaagaaag
     8281 atgccgaaac ggttaaaaaa caagcggtaa aaccgtctaa agaaacagag aaaaaagctc
     8341 caaaagaaga gaaaaaggcg gaaaaggaaa aagttgcacc caaaccgacc ccggaacaaa
     8401 tcctcaacag cggcagcatc gaaaaagcgc gcagtgccgc cgccaaagaa gtgcagaaaa
     8461 tgaaaacgtc cgacaaggcg gaagcaacgc attatctgca aatgggcgcg tatgccgacc
     8521 gccggagcgc ggaagggcag cgtgccaaac tggcaatctt gggcatatct tccaaggtgg
     8581 tcggttatca ggcgggacat aaaacgcttt accgggtgca aagcggcaat atgtctgccg
     8641 atgcggtgaa aaaaatgcag gacgagttga aaaaacatga agtcgccagc ctgatccgtt
     8701 ctatcgaaag caaataatta tgaagctcaa acatctgttg ccgctgctgc tgtcggcagt
     8761 gttgtccgcg caggcatatg ccctgacgga aggggaagac tatcttgtgt tggataaacc
     8821 cattcctcaa gaacagtcgg gtaaaattga ggttttggaa tttttcggct atttctgcgt
     8881 acattgccat catttcgatc ctttgttatt gaaactgggc aaggcattgc cgtctgatgc
     8941 ctatttgagg acggagcacg tggtctggca gcttgaaatg ctcggtctgg caagaatggc
     9001 ggctgccgtc aatttgtcgg gtttgaaata tcaggcaaac cctgccgtgt ttaaagcagt
     9061 ttacgaacaa aaaatccgct tggaaaacag ggctgttgcc ggaaaatggg ctttgtctca
     9121 aaaaggcttt gacggcaaaa aactgatgcg cgcctatgat tcccccgaag ctgccgccgc
     9181 cgcattaaaa atgcagaaac tgacggaaca ataccgcatc gacagcacgc cgaccgttgt
     9241 cgtcggcgga aaataccgcg ttatcttcaa taacggcttt gacggcggcg ttcatacgat
     9301 taaagaattg gttgccaaag tcagggcgga gcgcaaactg tcaaaccatc ctgtgcaaaa
     9361 ataaccgcat ccccgcgcat ttgaaagaaa ccaagcaatg gattttctga ttgtcctgaa
     9421 agccctgatg atgggcttgg tagaaggttt taccgaattt ttacctattt ccagcaccgg
     9481 acatttgatt gtgttcggta acttggtcgg ttttcacagc aaccacaagg tttttgaaat
     9541 tgccatccag cttggtgcgg ttttggcagt agtgtttgaa taccggcagc gtttcagcaa
     9601 tgtgttgcac ggcttaggaa aagaccggaa agccaaccgt ttcgtcctca atcttgccat
     9661 tgcttttata cctgccgccg tgatggggct gttgttcggc aaacaaatca aagagtatct
     9721 gtttaacccc ttgagtgttg cagtcatgct ggttttgggc ggttttttta ttttgtgggt
     9781 ggagaaacgc caaagccgag cagagcctaa aattgccgat gttgatgcat tgcgtccgat
     9841 tgatgccttg atgatcggta tcgcgcaggt gtttgccttg attccgggaa catcccgctc
     9901 gggcagtacg attatgggcg ggatgctttg gggaatcgag cggaaaacgg caacggagtt
     9961 ttcatttttc ttggccgttc cgatgatggt tgccgcaacg gcttatgatg tcctgaaaca
    10021 ttaccggttt ttcaccctgc atgatgtcgg tttgattctg ataggcttta ttgccgcttt
    10081 tgtttccggt ttagtagcgg tcaaagcact gctgaagttt gtttccaaga aaaactatat
    10141 cccgtttgcc tattaccgca ttgttttcgg catcgtcatc ataatattgt ggttgtcggg
    10201 ctggataagt tgggaatgaa accataaacc cgacctgaag acattattcg ggtcgggttt
    10261 gtctggcggg ctgatatagt gaattaacaa aaatcaggac aaggcgacga agccgcagac
    10321 agtacagata gtacggcaag gcgagccaac gctgtaccgg tttaaattta attcactata
    10381 aaatcaggac aggcggggcg ataggtttaa agtcgattgc ctgttttgaa ggcagtggtt
    10441 tattctttat ttgccggtaa tcaggtaata aaaaagcaca taccttttta cggtctgtgc
    10501 ttttttatct ggtggaggta agcgggatcg aaccgctgac ctcttgcatg ccatgcaagc
    10561 gctctaccaa ctgagctata cccccgaaaa tttggtggcg aatcagggac tcgaaccccg
    10621 gacacaagga ttatgattcc tctgctctaa ccgactgagc taattcgccg tttcgtgaag
    10681 acgctattat atgtttttct gtttttttga caagccgtat tttttaattt tgaattagtt
    10741 gactgttttt aaatgttaaa aagtttatgc cgtctgaagc ggattcaggc ggcatgaggg
    10801 ttagagtttg tggcagatgt cgccgaagcg gaatcctgcc cagtcgatgc cgatattttt
    10861 tccgaatgcg atgactttaa acagttcgcc catttcatgc tggtcaatca gtttctgaac
    10921 ggcagcagct tcacggatat aggctgccga attcgttttc cccgtctgtg ccaatagctc
    10981 ggtaatgccc aggttcaata agaaatggga ttggggaagg taacctatca aatctaatcc
    11041 ggcatccgtc ccggcttggg caatgtcggt aaagttgaca tgcgcggtca ggtcggccaa
    11101 tccgatgaag tcaaaaggat tgtggataac gtgatgtcgg tagtgtccga tcagagtacc
    11161 ttgattgcgt tgagggtggt aatactgcgc tgcatcaaaa ccgtagtcga tgaatatcat
    11221 gcagccgtgt tcgagtctgg aggcaagggt gcggataaag gcatattgtt gcggatgtag
    11281 ttcgctggtg tagggataat ctgtttgagg aaaatagagg gaagccaagg cagatagctg
    11341 taagtcgtgc agcggtcgtg ccgaataggt aaaacggtca ttatctaggc aaacgccgac
    11401 atgctcgaat gagccgcctt catttttacg gacgatttcg acaggcatgg catcgagtac
    11461 ttcgttgccg atgatgatgc cgtcaaacgc ttcgggaagt gcggtcaagt ggacaacttt
    11521 ttgagatgct tccggtgcgc gggcttgaat caggtttttc tgacgtgctg ccagctccgg
    11581 cgatatttca ataatatagt aacggctgat gccgtccgaa atgctgccca acaaatcggc
    11641 ggcaagctgt ccggttcccg cgccgaattc atagatattg cccgccgttt gggatagaag
    11701 ttcttgaagt tggcgtgcca gtgtctgtgc aaacagagag gtgagggtcg gtgcggtaat
    11761 aaaatccccg gtattgccga ttttatggct gccgccggtg tagtagccgt attgcggagc
    11821 gtataaaacc aattccataa aacgtgaaaa tggaatccag ttgccgtgtt tgccgatttt
    11881 ttcggcaatg agggtttgca gtttgagcga ggattgccgt gcttcgggag aggggagagg
    11941 gaggggcatg ataagtgtta gcttgtgtaa atttattgga tttcccgaca tattacacgt
    12001 tggtacgggt gctgtcatgg ctttatctta atactatata ttgtgtttat attattaaat
    12061 taatcatata tagttgttta ttggttcgat tattctgtac cgcacccgcc gtgccgttgt
    12121 cgtcattttt tatcttattg tttttaaaag gaataaaaat ttcagatatg ttaatgagtt
    12181 ttcatgccct gatttgaccg agtgtttaaa atttcttata gtgtcgattg gtggggaatt
    12241 gtggggcaaa gtgtctcttt tacccttgtg attttgattt cggcttggga catgtcatgt
    12301 tcggcggcgc acacgaatta agcatcgaca gtaaggggcg gttggctgtt cctgccaaat
    12361 tccgtgacat tctgtcgcgc ctctatacgc ctgccgtagt ggtaacgctc gagtcgaaac
    12421 acaagctgtt gatgtaccct gttgcggagt gggaaaaggt tgcggcgcaa cttttaaact
    12481 taaaagtggc ggataaccct gttttacggc ggtttcaaaa tcttctgctg cataacgcgg
    12541 aaattttgga atgggacagc gccggccggg tgctggttcc tgccggactg aggaagaggg
    12601 tggatttcga ccgtgaagtc gttttggtcg gtcgtgccaa ccgtttggag ctttggggtc
    12661 gcgagcagtg ggaggctgag atggttcagg ctttggatga cgatcctgac gaacttgcct
    12721 tccagttggg tcagacggat ttgcaattgt gagtggagca gaaagttacc ggcatatcac
    12781 ggtcttgctg aatgaggcgg tggatgcgct tgccgtgcgc gaagacggtg tctatgtgga
    12841 cggtacgttc ggcaggggag ggcattcccg gctgattttg tcgcgtttgg gcgatgcggg
    12901 gcggttgatt gttttcgaca aagaccctca ggcgattgct gtggcagaag agctggcgcg
    12961 ttcggacaaa cgggtcggtg tcgtgcatgg cggttttgct tcgtttcaga cggcattgga
    13021 cggtttgggt atcggcaagg tggacggtgc gctgtttgat ttggggattt cgtctccgca
    13081 aatcgatgac ggcagccgcg gtttcagctt tcgtttcgat gcccctttgg atatgcgtat
    13141 ggatacgacg cgcggtatgt ctgccgcaga gtggatagcg gttgcgtcgg aacaggattt
    13201 gcacgaggta atcaagaatt atggtgaaga gcggtttagc cgccagattg cgcgcgccat
    13261 tgttgcgcaa cgggcggaaa gtccaatcga tacaacccgc aagctggcgc agatcgtggc
    13321 acaaaacgtc cgtactcgcg agcgggggca ggatcctgcg acgcgcacct tccaggcggt
    13381 ccgcatcttt attaaccgcg agcttgaaga agtaggggca gtattgccgc aggtcatgtg
    13441 tcgtctgaaa gagggcggac gtttggcggt cattgctttc cattcgttgg aagaccgcat
    13501 tgtgaagcag tttgtcaaaa aatattcgca acacgcgccc ctgccgcgct gggcggcggt
    13561 cagggaagcg gatttgcccg agccgcccct gaaaatcgtg ggcagggcat taaagccggg
    13621 tgaggcggaa attgccgcca atccgagggc gagaagtgcg gttttgcgtg tggcggagcg
    13681 gactgccggt ccgataccgg aacaatcaca gagaaaaacg tctgaatggc aatgaacaaa
    13741 ttgaatttcc ttctgctgct tgcggtgtgc gtttccgctt tttccgttgt gatgcagcaa
    13801 aaccagtaca ggctcaactt cacagctttg gacaaggcga aaaaacagga aatcgccttg
    13861 gagcaggatt atgcgcaaat gaggctgcaa caggcgcgtt tggcgaacca cgaagcgatc
    13921 agggcggcgg cagaaaaaca aaacctccat ccgccggttt cgggcaatac ctttatggtg
    13981 gagcatcaaa gatagaagca gcctgtgtgc cggaatcgga ttcctgcgtc aggataataa
    14041 taacgagaag taaaaatgtt gattaagagc gaatataagc ctcggatgct gcccaaagaa
    14101 gagcaggtca aaaagccgat gaccagtaac ggacggatta gcttcgtcct gatggcaata
    14161 gcggtcttgt ttgccggtct gattgcccgc ggactgtatc tgcagacggt aacgtataac
    14221 tttttgaaag aacagggcga caaccggatt gtgcggactc aaacattgcc ggctacacgc
    14281 ggtacggttt cggaccggaa cggtgcggtt ttggcgttga gtgcgccgac ggagtccctg
    14341 tttgccgtgc ctaaagagat gaaggaaatg ccgtctgccg cacaattgga acgcctgtcc
    14401 gagcttgtcg atgtgccggt tgatgttttg aggaacaagc tcgaacagaa aggcaagtcg
    14461 tttatctgga ttaagcggca gctcgatccc aaggttgccg aagaggtcaa agccttgggt
    14521 ttggaaaact ttgtatttga aaaagaatta aaacgccatt acccgatggg caacctgttt
    14581 gcacacgtca tcggatttac cgatattgac ggcaaaggtc aggaaggttt ggaactttcg
    14641 cttgaagaca gcctgcatgg cgaagacggc gcggaagtcg ttttgcggga ccggcagggc
    14701 aatattgtgg acagcttgga ctccccgcgc aataaagccc cgaaaaacgg caaagacatc
    14761 atcctttccc tcgatcagag gattcagacc ttggcctatg aagagttgaa caaggcggtc
    14821 gaataccatc aggcaaaagc cggaacggtg gtggttttgg atgcccgcac gggggaaatc
    14881 ctcgccttgg ccaatacgcc cgcctacgat cccaacaggc ccggccgggc agacagcgaa
    14941 cagcggcgca accgtgccgt aaccgatatg atcgaacccg gttcggcaat caaaccgttt
    15001 gtgattgcga aggcattgga tgcgggcaaa accgatttga acgaacggct gaatacgcag
    15061 ccttataaaa tcggaccgtc tcccgtgcgc gatacccatg tttacccctc tttggatgtg
    15121 cgcggcatca tgcagaaatc gtccaacgtc ggcacaagca aactgtctgc gcgtttcggt
    15181 gccgaagaaa tgtatgactt ctatcatgag ttgggcatcg gtgtgcgtat gcactcgggc
    15241 tttccgggcg aaactgcagg tttgttgaga aattggcgca ggtggcggcc cattgaacag
    15301 gcgacgatgt ctttcggtta cggcctgcaa ttgagcctgc tgcaattggc gcgcgcctat
    15361 accgcactga cgcacgacgg cgttttgctg ccggtcagct ttgaaaaaca ggcggttgcg
    15421 ccgcaaggca aacgcatatt caaagaatcg accgcgcgcg aggtacgcaa tctgatggtt
    15481 tccgtaaccg agccgggcgg caccggtacg gcgggtgcgg tggacggttt cgatgtcggc
    15541 gcgaaaaccg gcacggcgcg caagttcgtc aacgggcgtt atgccgacaa caaacacatc
    15601 gctaccttta tcggttttgc ccccgccaaa aacccccgtg tgattgtggc ggtaaccatt
    15661 gacgaaccga ctgcccacgg ttattacggc ggcgtagtgg cagggccgcc cttcaaaaaa
    15721 attatgggcg gcagcctgaa catcttgggc atttccccga ccaagcccct gaccgccgca
    15781 gccgtcaaaa caccgtctta atccgagtat caacgagatt gttttatgtt cagcaagtta
    15841 acccctttgg ctgaaaccgg catcccgacc ctgtcgtgtg caaacgcggc agggcgtttg
    15901 ttgcattcag acagccgcca aatcaaacaa ggcgatattt tcgttgcctg tccgggcgaa
    15961 tatgccgacg gacgcagtta tatccccgcc gccgttgcca acggcgcggc ttttgttttt
    16021 tgggacgacg acggcaaatt tgcgtggaat cccgaatgga aagtccccaa tcaaggcatc
    16081 aaagatttga aacaccgtgc cggcatattg gcggcgcaag tttacggcaa cgtttcagac
    16141 ggtctcaaat tctggggcgt aaccggaacc aacggcaaaa cctccatcac gcaatggctg
    16201 gcgcaagctg ccgatttgtt gggcgaaaaa accgccattg tcggcacggt cggcaacggc
    16261 ttttggggtg cattggaaga aaccacgcat accacacccg cccccgtcga tgtccaaacc
    16321 ctgctctacc gtttccgtca acaaggcgca acagtcgccg cgatggaagt ctccagccac
    16381 gggcttgacc agtcgcgcgt caacggcgtg tcattccgca gcgcaatctt taccaacctc
    16441 acccgcgacc acctcgacta ccacggcacg atggaagcct acggtgccat caagtcgcgc
    16501 ctgttttact ggcacggctt gaaacacgca gtcatcaacg tggatgacga atacggcgcg
    16561 gaactcgtag gtcgtctgaa aaaagactgt cccgatttgg ccgtttatag ctacggcttc
    16621 agcgaacacg ccgacatccg cattatcgat ttcaccgcct cttcagacgg catagcagcc
    16681 gtattccaaa ccccgtgggg cgaagggaaa tgccgcacgc gcctgctcgg acggttcaac
    16741 gcgcaaaacc tcgccgcctg catcgccttg ctgtgcgcca acggctatcc gcttgataaa
    16801 gtattggatg tgctggcaaa aatccgtccc gcttcagggc gcatggactg catcatgaac
    16861 agcggcaaac ctttggtcgt tgtcgattat gcccatacgc ccgacgcatt ggaaaaagca
    16921 ctcgccacct tgcaggaaat caaaccgcag ggtgcggctt tgtggtgcgt attcggttgc
    16981 ggcggtaacc gcgatcgcgg caaacgcccg ctgatgggcg cggcagccgt acagggcgcg
    17041 gataaagtcg tcgtcaccag cgacaacccg cgtttggaaa atccgcacga catcatcaac
    17101 gacatcctgc ctgccgttcc cgcgcccgaa tgcgtcgaag ccgaccgtgc cgccgccatc
    17161 cgttatgcgg ttgaacaagc cgccgcaaac gacatcatcc tgattgccgg caaagggcat
    17221 gaaaactatc aggatgtaca aggcgtgaag caccgttttt ccgatcttga aatcgtcgga
    17281 caggctttgt taactcgtaa ataatgggat attcggacgg catcgtatga aacaatccgc
    17341 ccgaataaaa aatatggatc agacattaaa aaatacattg ggcatttgcg cgcttttagc
    17401 cttttgtttt ggcgcggcca tcgcatcagg ttatcacttg gaatatgaat acggctaccg
    17461 ttattctgcc gtgggtgctt tggcttcggt tgtattttta ttattattgg cacgcggttt
    17521 cccgcgcgtt tcttcagttg ttttactgat ttacgtcggc acaaccgccc tatatttgcc
    17581 ggtcggctgg ctgtatggtg cgccgtctta tcagatagtc ggttcgatat tggaaagcaa
    17641 tcctgccgag gcgcgtgaat ttgtcggcaa tcttcccggg tcgctttatt ttgtgcaggc
    17701 attatttttc atttttggct tgacagtttg gagatattgt gtaccggggg ggtatttgct
    17761 gacgtaaaaa actataaacg ccgcagcaaa atatggctga ctatattatt gactttgatt
    17821 ttgtcctgcg cggtgatgga taaaatcgcc agcgataaag atttgcgaga acctgatgcc
    17881 ggcctgttgt tgaatatttt cgacctgtat tacgatttgg cttccgcgcc ggcacaatat
    17941 gccgccaagc gcgcccacat tttggaagca gcaaaaaaag cgtcaacatg gcatatccgt
    18001 catgttgcgc ccaagtataa aaattatgtt gtggttatcg gtgagagcgc gcgttcggat
    18061 tatatgaatg tttacggttt cccattgccc gatacgcctt ttttgagtca gaccaaaggg
    18121 ctgttgataa acggttacca atcgaccgcc cacgcgacga atctttcgct gccgcagact
    18181 ttggggctgc cgggagaacc gaacaataac atcgtcagct tggcgaagca ggcgggtttt
    18241 cggacggcgt ggctgtctaa tcaaggaatg ttggggcatt ttgccaacga aatttccacc
    18301 tatgccctac gcagcgatta tccgtggttt acccaaaggg gtgattatgg caaaagcgcg
    18361 gggttgagcg accgcctttt gttgccggcg ttcaaacggg ttttgatagg aaatgcaggc
    18421 acgaagcctc ggctgattgt gatgcacctg atgggttcgc acagtgattt ttgcacacgt
    18481 ttggataagg atgcgcggcg gtttcagtat caaactgaaa aaatatcctg ctatgtttcc
    18541 accatcgcgc aaaccgataa atttttagaa gatacagtta agatattgaa tgaaaataaa
    18601 gaaagctggt ctttggttta cttttccgac cacggtttga tgcatgtcgg taaaggcggc
    18661 gagcgaacgt tgacacatgg tgcgtggaag cgtcaaagct acggcgtgcc gctggttaaa
    18721 atttcgtccg atgatacgcg gcgcgaaatg attaaagtga ggcgcagcgc gtttaatttt
    18781 ttacgcggat tcggcagttg gacgggtatc gaaaccgacg agttgcccga tgacggctat
    18841 gatttttggg ggaatgttcc cgatgtgcag ggcgaaggca ataaccttgc ctttatcgac
    18901 ggactgcccg acgaccccgc gccgtggtat gcgggaaaag gcaaatcgac taaaaatacg
    18961 tctaaaaaat gatacgtaca gaaaaaatgc cgaatgagaa tgggaaaata atctgtgttt
    19021 taccacagca aaacaggcga taaaaaaatc agccgctacc gatgtgtccg ccgcccgaat
    19081 attaacgaaa gtaaatatga aaccactgga cctaaatttc atctgccaag ccctcaagct
    19141 tccgatgccg tctgaaagca aacccgtgtc gcgcatcgta accgacagcc gcgacatccg
    19201 cgcgggcgat gtgtttttcg cattggcggg cgggcggttt gatgcgcatg attttgttga
    19261 agacgtattg gctgcgggtg cggcggcggt tgtggtttcg cgcgaagatt gcgttgcaat
    19321 ggatggcgcg ttgaaagtcg atgacacgct taccgcgttg caaatgttgg cgaaggcgtg
    19381 gcgcgagaat gtgaacccgt ttgtgttcgg tattaccggc tcgggcggca agacgacggt
    19441 gaaggaaatg ttggctgcgg tattgcgccg ccgtttcggc gataatgccg ttttggcgac
    19501 ggcaggcaac ttcaacaacc acatcggatt gccgttgact ttgttgaaat taaacgaaaa
    19561 acaccgctat gccgtgattg aaatgggtat gaaccatttt ggcgaactgg cggttttgac
    19621 acaaatcgcc aaacccgatg ccgcattggt caacaacgcc atgcgcgccc atgtcggctg
    19681 cggtttcgac ggagtgggcg atattgccaa agcgaaaagc gagatttatc aaggcttatg
    19741 ttcagacggc atggcgctga ttcctcaaga agatgccaat atggctgtct tcaaaacggc
    19801 aacgcttaat ttgaatacgc gcactttcgg catcgatagc ggcgatgtcc acgcggaaaa
    19861 tatcgtgctg aaaccgttgt cgtgcgaatt tgatttggtg tgcggcaacg agtgcgcagc
    19921 cgtggttctg cccgttcccg gccgccacaa tgtccacaac gccgccgccg ccgccgcgct
    19981 gtctttggct gcaggtttga gtttgaacga tgtggcggaa ggtttgaaag gcttcagcaa
    20041 tatcaaaggc cgtctgaacg tcaaatccgg aatcaagggc gcaaccctga ttgacgatac
    20101 ttataatgcg aaccctgaca gcatgaaagc tgcggttgac gtgttggcgc gtatgcctgc
    20161 gccgcgtatt ttcgtgatgg gcgatatggg cgaactgggt gaggacgaag ccgccgccat
    20221 gcacgccgaa gtcggcgcgt acgcccgcga ccaaggcatc gaagcggctt attttgtcgg
    20281 cgacaacagc gtcgaagcgg cggaaaaatt tggcgcggac ggtttgtggt tcgccgccaa
    20341 agacccgttg attcaagtgt tgcgccacga tttgcccgaa cgcgccaccg tgttggtgaa
    20401 aggttcgcgc tttatgcaga tggaagaagt ggtcgaggca ttggaggata agtgaaaatg
    20461 aaaagtcgac gtttttttaa agccttattg ttgattgccg cgctggtcgg cgcgttttat
    20521 gccggaatgc agacgcaggc gtatctttat gaagatttat gtttagactt gggtggcggc
    20581 aaaaatccgg ggagttaccc gatttgcgtg attgggaaag tctctgcacg ttaatctgca
    20641 aaagtcgtcc gaaaccttgc cgggcggcaa gccaacctca aacgggcaca agcccatgta
    20701 acgacaaaaa caaaaaaagg aagccccatg tttttatggc tcgcacattt cagcaactgg
    20761 ttaaccggtc tgaatatttt tcaatacacc acattccgcg ccgtcatggc ggcgttgacc
    20821 gccttggcgt tttccctgat gttcggcccg tggacgatac gcaggctgac cgcgctcaaa
    20881 tgcgggcagg cagtgcgtac cgacggtccg caaacccacc tcgtcaaaaa cggcacgccg
    20941 acgatgggcg gttcgctgat tctgaccgcc attaccgtgt ccaccctgtt gtggggcaac
    21001 tgggcaaacc cgtatatctg gattctcttg ggcgtattgc tcgccacggg cgcactcggt
    21061 ttttacgacg actggcgcaa agtcgtctat aaagacccca acggcgtgtc cgccaaattc
    21121 aaaatggtgt ggcagtcaag cgttgccatt atcgccggtt tggcattgtt ttaccttgcc
    21181 gccaattccg ccaacaatat tttgattgtc ccgttcttca aacaaatcgc cctgccgctg
    21241 ggcgtggtcg gctttttggt gttgtcttac ctgaccatcg tcggcacatc caatgccgtc
    21301 aacctcaccg acggcttgga cggccttgcg accttccccg tcgtcctcgt tgccgccggc
    21361 ctcgccatct tcgcctatgc cagcggccac tcacaatttg cccaatacct gcaattacct
    21421 tacgttgccg gcgcaaacga agtggtgatt ttctgtaccg ccatgtgcgg cgcgtgcctc
    21481 ggtttcttgt ggtttaacgc ctatcccgcg caagtcttta tgggcgatgt cggtgcattg
    21541 gcattgggtg ccgcgctcgg taccgtcgcc gtcatcgtcc gccaagagtt tgtcctcgtc
    21601 attatgggcg gattatttgt cgtagaagcc gtatccgtta tgcttcaggt cggctggtat
    21661 aagaaaacca aaaaacgcat cttcctgatg gcgcccatcc atcaccacta cgaacaaaaa
    21721 ggctggaaag aaacccaagt cgtcgtccgc ttttggatta ttaccatcgt cttggtgttg
    21781 atcggtttga gtaccctcaa aatccgctga acctatgccg tctgaacacc tttcagacgg
    21841 catttgaacg cgcaataaac ctgcggcgac aatccgccca gccctatcgt taacggcggc
    21901 ttatactaaa acagaagtaa aaccatgaaa cagacagtca aatggcttgc cgccgccctg
    21961 attgccttgg gcttgaacca agcggtgtgg gcggatgacg tatcggattt tcgggaaaac
    22021 ttgcaggcgg cagcacaggg aaatgcagca gcccaaaaca atttgggcgt gatgtatgcc
    22081 gaaagacgcg gcgtgcgcca agaccgcgcc cttgcacaag aatggcttgg caaggcttgt
    22141 caaaacggat accaagacag ctgcgacaat gaccaacgcc tgaaagcggg ttattgaaca
    22201 gctcgcgatg ccgtctgaaa gcggcttggg caggggcgga catctcctgc tcaatatgat
    22261 ttgttttagg acaaaccaaa atgacttttc aaaacaaaaa aatcctcgtc gccggactcg
    22321 gcggtacggg tatttccatg attgcctacc tgcgcaaaaa cggcgcggag gttgctgcgt
    22381 atgatgcgga gctgaagccg gaacgcgtgt cgcaaatcgg taagatgttt gacgggttgg
    22441 tgttttacac gggccgtctg aaagatgcgc tggacaacgg tttcgatatt ctggcactca
    22501 gccccggcat cagcgagcgg cagccggata ttgaggcgtt caagcaaaac ggcggacgcg
    22561 tgttgggcga catcgaattg ctggcggaca ttgtgaaccg ccggggcgac aaggtaattg
    22621 cgattaccgg cagcaacggc aaaaccacgg taacgagcct agtcggctat ctctgcatca
    22681 agtgcgggct ggataccgtt atcgcgggca atatcggcac gccggtttta gaggcggaat
    22741 tgcagcgtga aggcaaaaag gcggacgtgt gggtgttgga gctttccagc ttccaactgg
    22801 aaaacaccga aagcctgcgc ccgactgcgg cgacggtgct gaacatttcc gaagaccatc
    22861 tcgaccgcta cgacgacctg ctcgactacg cgcataccaa ggacaagatt ttccgtggcg
    22921 atggcgttca ggttttgaat gcggacgata cgttctgttg tgccatgaag cgcgccgggc
    22981 gcgaggtaaa atggttttcg ttggaacacg aagccgattt ttggttggaa cgcgagacag
    23041 gccgcctgaa acaaggcaat gaagatttga ttgccacgca agacattccg ttgcaaggcc
    23101 tgcacaatgc cgccaacgtg atggctgccg tcgccttgtg cgaagccgtc ggtttgccgc
    23161 gaaatgaatt gctcgaacac gtcaaaacct ttcaaggcct gccgcaccgc gtggaaaaaa
    23221 tcggcgagaa aaacggcgtg gtgttcatcg acgacagcaa aggcacgaac gttggtgcaa
    23281 ccgccgctgc gattgccggt ttgcaaaatc cgctattcgt cattttgggc ggcatgggca
    23341 aagggcagga cttcacgccc ctgcgcgacg cgcttgccgg caaggcaaaa ggcgtgttcc
    23401 tgattggcgt cgatgcgccg caaatccgcc acgatttgga cggctgcggt ttgaatatga
    23461 ccgactgcgc cactttggaa gaagcggttc agaaggcata tgcccaagcc gaagcgggcg
    23521 atatcgtgct gctcagcccc gcctgcgcga gcttcgatat gtttaaaggc tatgcgcacc
    23581 gttcggaagt gtttatcggg gcgtttaagg ctttgtgata ccgtctgaaa cgcaaacgcc
    23641 gtcattgttg ggcggcaagt aaagatttag aataccgatt tgggatgtat cgtatgttcg
    23701 gacggcattg tctgccgtct gaaatttttg ccctttgcgg caggtgcaaa cagactggca
    23761 ggtggttttt ttgaagattt cggaagtatt ggtaaaagtg ggcgacggtg tccacactct
    23821 gctgctcgac aggccgattg tgcgcgacgg caggaaattc gacgcgccgc ttttgtggat
    23881 ggtggtgctg atgacggcgt tcagcctgct gatgatttat tcggcttctg tgtatttggc
    23941 atcaaaagaa ggcggcgatc agtttttcta tttgaccaga caggcggggt tcgtcgttgc
    24001 cggcttgata gcgagcggtt ttttatggtt tctttgcagg atgaggacat ggcggcggct
    24061 tgtgccgtgg atttttgccc tatccggcct gttgctggta gtcgtattga ttgccgggcg
    24121 cgaaatcaat ggcgcgaccc gttggatacc tttgggtccg ttgaatttcc agccgaccga
    24181 gctgttcaag ctggcggtca tcctttattt ggcaagcctg ttcacgcgcc gtgaagaagt
    24241 gttgcgcagc atggaaagtt tgggttggca gtcgatttgg cgggggacgg ccaatctgat
    24301 catgtccgcc accaatccgc aggcacgtcg tgaaacatta gaaatgtacg gccgtttccg
    24361 ggcgatcatc ctgccgatta tgctggtggc gttcggtttg gtgctgataa tggtacagcc
    24421 ggatttcggt tcgtttgtcg tcattaccgt cattgccgtt ggaatgctgt ttttggcagg
    24481 attgccgtgg aaatattttt tcgtcctggt aggcagcgtc ttgggcggga tggtgctgat
    24541 gattaccgcc gctccctacc gtgtgcagcg ggtagtggca tttttggacc cgtggaaaga
    24601 cccgcagggt gccggctacc agcttaccca ctctctgatg gcaatcgggc gcggagagtg
    24661 gttcggtatg ggtttgggtg cgagtttgag caaacgcggc tttctgccgg aagcgcatac
    24721 cgattttatt tttgccatca tcgccgaaga attcggtttc ttcggtatgt gcgtgctgat
    24781 attctgttac ggctggctgg tggtgcgggc gttttccatc ggcaagcagt cgcgcgattt
    24841 gggtttgact ttcaacgcct atatcgcttc gggtatcggc atttggatcg gtatccaaag
    24901 tttcttcaat atcggtgtga acatcggtgc tttgccgacc aaaggtctga cgctgccgtt
    24961 gatgtcctat ggcggttcgt cagtcttttt catgctgatc agcatgatgc tgctgttgcg
    25021 tatagattat gaaaaccgcc ggaaaatgcg cggttatcgg gtggagtaaa tcatgggcgg
    25081 taaaaccttt atgctgatgg cgggcggaac gggcggacat attttccccg cgctggcggt
    25141 ggcggattca ttgcgcgcgc gcggccatca tgtgatttgg ctgggcagca aggattcgat
    25201 ggaagagcgt atcgtgccgc aatacggcat acgcttggaa acgctggcga ttaaaggcgt
    25261 gcgcggcaac ggcatcaaac gcaagctgat gctgccgttt actttgtatc aaaccgtccg
    25321 cgaagcgcag cggattatcc gcaaacaccg tgtcgagtgc gtcatcggct tcggcggctt
    25381 cgttaccttc cccggcggtt tggcggcgaa actcttgggc gtgccgattg tgattcacga
    25441 gcaaaacgcc gtggcaggtt tgtccaaccg ccacctgtcg cgctgggcga agcgggtgtt
    25501 gtacgctttt ccgaaagcgt tcagccacga aggcggcttg gtcggcaacc ccgtccgcgc
    25561 cgatattgcc gggctgcccg cgcctgacga acgcttccaa gggcgcgaag gccgtctgaa
    25621 aattttggtg gtcggcggca gtttgggtgc agacgttttg aacaaaaccg taccgcaggc
    25681 attggctttg ctgcccgaca atgcgcgtcc gcagatgtac caccaatcgg ggcgcaacaa
    25741 gctgggcagc ttgcaggcgg attacgacgc gctgggcgtg aaagccgaat gcgtggaatt
    25801 tattaccgat atggtgtccg cctaccgcga tgccgatttg gtgatttgcc gtgccggcgc
    25861 gctgacgatt gccgagttga cggcggcggg attgggtgcg ttgttagtgc cgtatcctca
    25921 cgccgttgat gaccatcaaa ccgccaacgc gcgttttatg gtgcaggcgg aggcgggatt
    25981 gctgttgccg caaacccagt tgacggcgga aaaactcgcc gaaatcctcg gcagcctgaa
    26041 ccgcgaaaaa tgtctcaaat gggcagaaaa cgcccgtact ttggcattgc cgcacagcgc
    26101 ggacgacgtt gccgaagccg cgattgcgtg tgcggcgtaa actgccgaac catgccgtct
    26161 gaaaagccgt tcagacggca tggatgtttt ttatttcaat ccgctatata tttgtcagaa
    26221 aactatggcg cgcaaacggt cagcccttta aaataacgcc tttacgcatc gaaaatccac
    26281 cggaacgcaa cattatgatg aaaaatcgag tgaccaacat ccattttgtc ggtatcggcg
    26341 gcgtcggcat gagcggcatc gccgaagtct tgcacaattt gggttttaaa gtttccggtt
    26401 cggatcaggc gcgaaatgcc gctaccgagc atttgggcag cctgggcatt caagtttatc
    26461 ccggccatac cgcagaacac gttaacggtg cggatgtcgt cgttacctct accgccgtca
    26521 aaaaagaaaa tcccgaagtt gtcgctgcgt tggagcagca aattcccgtt attccgcgcg
    26581 ccctgatgtt ggcggagttg atgcgcttcc gtgacggcat cgccattgcc ggcacgcacg
    26641 gcaaaaccac gaccaccagc ctgaccgcct ccatcctcgg cgcggcagga cttgacccga
    26701 ctttcgttat cggcggcaaa ctcaacgccg caggtaccaa cgcccgtttg ggcaaaggcg
    26761 aatacattgt cgccgaagcc gacgaatccg atgcctcttt cctacatcta accccgatta
    26821 tgtctgtcgt caccaatatc gacgaagacc acatggatac ctacggacac agcgttgaaa
    26881 aactgcatca ggcgtttgtt gattttatcc accgtatgcc gttctacggc aaagcgtttt
    26941 tgtgtatcga cagcgaacac gtccgcgcga ttctgcccaa agtgagcaaa ccttatgcca
    27001 cttacggctt ggacgatacc gccgacatct acgccaccga catcgaaaac gtcggcgcgc
    27061 aaatgaaatt caccgtccat gttcaaatga aaggacatga gcaggggtcg tttgaagtcg
    27121 tgctgaatat gcccggccgc cacaatgttc tcaatgcctt ggcagccatc ggtgttgcgt
    27181 tggaagtcgg cgcatcggtt gaagccatcc aaaaaggctt gctcggcttt gaaggcgtcg
    27241 gccgccgctt ccaaaaatac ggcgacatca agttgccaaa cggcgggacc gcgctcttgg
    27301 tggacgacta cggacaccac cccgtcgaaa tggcggcgac cctttccgcc gcacgcggcg
    27361 cgtatccgga aaaacgtttg gtactcgcct tccagccgca ccgctatacc cgcacgcgcg
    27421 atttgtttga agactttacc aaagtcctca ataccgttga cgcgctggtg ctgaccgaag
    27481 tgtatgccgc cggtgaagag ccgattgccg ccgccgattc ccgcgctctt gcccgcgcca
    27541 tccgcgtgtt gggcaaactc gagccgattt actgcgaaaa cgttgccgat ctgcccgaaa
    27601 tgctgttgaa cgttttgcag gacggcgaca tcgtgttgaa tatgggcgcg ggaagcatca
    27661 accgcgtccc cgccgcgctg ctggaattgt cgaaacagat ttgaggcaca cccgcctgac
    27721 agacggaaca tcatatcaag gtcgtctgaa accgcaaatc aggtttcaga cgacctctgg
    27781 taacaagcat aaagcaatca ggaaagaaca aaaacaatgc agaattttgg caaagtggcc
    27841 gtattgatgg gcggtttttc cagcgaacga gaaatctcgc tggacagcgg caccgccatt
    27901 ttgaatgctt taaaaagcaa aggcatagac gcatacgcct tcgatcctaa agaaacccca
    27961 ttgtctgaat tgaaggcaca aggttttcag acggcattca acatccttca cggtacttac
    28021 ggcgaagacg gggcggttca gggtgcattg gaactgttgg gcattcccta taccggcagc
    28081 ggtgtcgccg catccgccat cggcatggac aaataccgct gcaaactgat ttggcaggca
    28141 ttgggattgc ccgttcccga gttcgccgtc ctgcacgacg acactgattt cgatgccgtc
    28201 gaagaaaaat tgggcctgcc gatgtttgtg aaaccggcgg ccgaaggcag cagcgtaggc
    28261 gtggtaaaag tcaaaggaaa aagccgtctg aaaagcgttt acgaagaatt gaaacacctt
    28321 cagggcgaaa tcattgccga acggtttatc ggcggcggcg aatattcctg ccctgtgttg
    28381 aacggcaaag gcctgcccag catacacatc atccccgcga ccgagtttta cgactacgaa
    28441 gccaagtaca accgcaacga caccatttat caatgtcctt cggaagatct gaccgaagcc
    28501 gaagaaagcc tgatgcgcga actggcggtt cgcggcgcgc aggcaatcgg tgcggaaggc
    28561 tgcgtgcgcg tcgatttcct caaagatacc gacggcaaac tctatctgtt ggaaatcaac
    28621 accctgcccg gtatgaccgg ccatagttta gtaccgaaat ccgctgccgt tacgggcgtg
    28681 ggttttgccg atttatgtat tgaaattttg aagaccgcac atgtgggata atgccgaagc
    28741 gatggaacgg ctgacgcgct ggctgcttgt catgatggcg atgctgcttg ctgcgtccgg
    28801 gctggtttgg ttttacaatt cgaatcatct gcccgtcaag caggtgtcgc tgaagggcaa
    28861 cctggtttat tccgataaga aagcattggg cagtttggcg aaagaataca tccatgggaa
    28921 tattttgagg acggacatca atggcgcaca ggaggcctac cgccggtatc cgtggattgc
    28981 gtcggtcatg gttcgccgcc gttttcccga cacggttgag gtcgtcctga ccgagcgcaa
    29041 gccggtcgcg cgttggggcg accatgcctt ggtggacggc gaaggcaatg tttttgaagc
    29101 ccgcttggac agacccggaa tgccggtatt cagaggcgcg gaaggaacgt ctgccgaaat
    29161 gctccgccgt tatgacgaat tttcgactgt tttggcaaaa cagggtttgg gcatcaaaga
    29221 gatgacctat acggcacgtt cggcgtggat tgtcgttttg gacaacggca tcaccgtcag
    29281 gctcggacgg gaaaacgaga tgaaacgcct ccggcttttt accgaagcgt ggcagcatct
    29341 gttgcgtaaa aataaaaatc ggttatccta tgtggatatg aggtataagg acggattttc
    29401 agtccgctat gcttacgacg gtttacccga aaaagaatcc gaagaatagt gggaacaggt
    29461 atcggacaga ttacggccgt gccgtctgaa acggtgcgac gcaaatttca atcagtttta
    29521 agagcagacg aacaatggaa cagcagcaaa gatacatcag cgtactggat atcggtacgt
    29581 ctaaagtcct cgcactgatc ggggaagttc aagatgacga caaaatcaac atcgtcggtt
    29641 tggggcaggc tccttcacgg ggcttgcgcg cgggcatggt aaccaatatc gatgccaccg
    29701 tccaagccat caggcaggcg gtcaatgatg ccgagctgat ggcggatacc aaaattaccc
    29761 acgttaccac aggtatcgca ggcaaccaca tccgcagtct caattcgcaa ggtgtggtta
    29821 aaattaaaga cggggaagtc acgcaggcag acatcgaccg cgccattgaa acggcaaagg
    29881 caatcaatat cccgcccgat caaaaaattc tcgatgctgt ggttcaagac tacattatcg
    29941 acacccaact tggcgtgagg gagcctatcg gtatgagcgg tgtgcgtttg gatacgcggg
    30001 tgcacatcat taccggtgca agtacggcag tgcagaatgt ccaaaaatgt atcgagcggt
    30061 gcggcttgaa aagcgatcag atcatgcttc agccgttggc aagcgggcag gcggtgctga
    30121 ctgaagatga aaaagacctc ggcgtatgcg tcatcgacat tggtggcgga acgaccgata
    30181 ttgccgttta tatgaacggc gccatccgcc atacgtccgt cattccggcc ggtggtaatc
    30241 tgattaccaa agatttgtcc aaatcgttga gaacacctct cgatgccgcc gagtacatta
    30301 aaatccatta tggcgtgaca tcatgcgata cggaaggctt gggtgagatg attgaagttc
    30361 cgggcgtggg tgaccggaca tcgcgtcagg tttccagtaa ggttttggca gcaatcatca
    30421 gtgcacgtat tcaggagatt tttggcgtag tgctgggcga gctgcaaaaa tcgggtttcc
    30481 ccaaagaagt gctgaatgcg ggtattgttc tgaccggcgg tgtgtccatg atgaccggga
    30541 ttgtggaatt tgccgaaaaa atcttcgatt tgcctgtacg caccggtgcg ccccaagaaa
    30601 tgggcggttt gtccgaccgc gtccgcacac cgcgcttttc taccgccatc gggctgcttc
    30661 atgcagcatg caagctggaa ggaaacttgc cgcagccgga aaacggtgca gtgcaagaga
    30721 gggaaggggg cggcggtttg ttggcaagat tgaaacggtg gattgaaaac agcttctgaa
    30781 caggtggatt gccgtttgac aggtgagaag tattttgcca gcagcaagat acttcttata
    30841 taatgaataa taatttattt aaaccgtcct ctgaatgggg cgagcaggag tttttgaatg
    30901 gaatttgttt acgacgtggc agaatcggca gtcagccctg cggtgattaa agtaatcggc
    30961 ttgggcggcg gcggttgcaa tgcaatcaat aacatggttg ccaacaatgt gcgcggtgtg
    31021 gagtttatca gtgccaatac ggatgcgcag tctctggcaa aaaaccatgc ggcgaagaga
    31081 atccagttgg gtacgaatct gacacgcggt ttgggcgcgg gcgcgaatcc cgatatcggc
    31141 cgtgcggcag cccaggaaga ccgggaagcc attgaagaag ccattcgcgg tgcgaatatg
    31201 ctgtttatca cgaccggtat gggcggcggt accggtaccg gttccgcgcc ggttgttgct
    31261 gagattgcca agtctttggg cattctgacc gttgccgtgg ttacccgacc gttcgcatat
    31321 gaaggtaagc gcgtccatgt cgcacaggca gggttagaac agttgaaaga acacgtcgat
    31381 tcgctgatca tcatcccgaa cgacaaactg atgactgcat tgggtgaaga cgtaacgatg
    31441 cgcgaagcct tccgtgccgc cgacaatgta ttgcgcgatg cggtcgcagg catttccgaa
    31501 gtggtaactt gcccgagcga aatcatcaac ctcgactttg ccgacgtgaa aaccgtgatg
    31561 agcaaccgcg gtatcgctat gatgggttcg ggttatgccc aaggtatcga ccgtgcgcgt
    31621 atggcgaccg accaggccat ttccagtccg ctgctggacg atgtaacctt ggacggagcg
    31681 cgcggtgtgc tggtcaatat tacgactgct ccgggttgct tgaaaatgtc cgagttgtcc
    31741 gaagtcatga aaatcgtcaa ccaaagcgcg catcccgatt tggaatgcaa attcggtgcg
    31801 gctgaagacg agaccatgag cgaagatgcc atccggatta ccattatcgc taccggtctg
    31861 aaagaaaaag gcgcggtcga ttttgttccg gcaagggagg tagaagcggt tgctccgtcc
    31921 aaacaggagc aaagccacaa tgtcgaaggt atgatccgca ccaatcgcgg tatccgcacg
    31981 atgaacctta ccgctgcgga tttcgacaat cagtccgtac ttgacgactt tgaaatccct
    32041 gcgattttgc gtcgtcaaca caattcagac aaataatgtg ctgtttgccc gtaaacctgc
    32101 tgcctcccga atcggtttgt ccggtttggg aggtatgttt ttcaagatgt tgcaatttcg
    32161 tacggtttgc ggtcggcgga ttcagatttt tccacttgat acagactttc agatatggac
    32221 acttcaaaac aaacactgtt ggacgggatt tttaagctga aggcaaacgg tacgacggtg
    32281 cgtaccgagt tgatggcggg tttgacaact tttttgacga tgtgctacat cgttatcgtc
    32341 aaccctctga ttttgggcga gaccggcatg gatatggggg cggtattcgt cgctacctgt
    32401 atcgcgtctg ccatcggctg ttttgttatg ggttttgtcg gcaactatcc gattgcactc
    32461 gcaccgggga tggggctgaa tgcctatttc acctttgccg tcgttaaggg tatgggcgtg
    32521 ccttggcagg ttgcgttggg tgcggtgttc atctccggtc tgatttttat cctgttcagc
    32581 ttttttaaag tcagggaaat gctggtcaac gcactgccta tgggtttgaa aatgtcgatt
    32641 gctgccggta tcggtttgtt tttggcactg atttccctga aaggcgcagg cattatcgtt
    32701 gccaatccgg caaccttggt cggtttgggc gatattcatc agccgtccgc gttgttggca
    32761 ctgttcggtt ttgctatggt ggtcgtattg ggacatttcc gcgttcaagg cgcaatcatc
    32821 atcaccatct tgaccattac cgtcattgcc agcctgatgg gtttgaatga atttcacggc
    32881 atcatcggcg aagtaccgag cattgcgccg acttttatgc agatggattt tgaaggcctg
    32941 tttaccgtca gcatggtcag tgtgattttc gtcttcttct tggtcgatct atttgacagt
    33001 accggaacgc tggtcggcat atcccaccgt gccgggctgc tggtggacgg taagctgccc
    33061 cgcctgaaac gcgcactgct tgcagactct accgccattg tggcaggtgc ggctttgggt
    33121 acttcttcca ccacgcctta tgtggaaagc gcggcgggcg tatcggcagg cggacggacc
    33181 ggcctgacgg cggttacagt cggcgtattg atgctcgcct gcctgatgtt ttcacctttg
    33241 gcgaaaagtg ttcccgcttt tgccaccgcg cccgccctgc tttatgtcgg cacgcagatg
    33301 ctccgcagtg cgagggatat tgattgggac gatatgacgg aagccgcacc cgcattcctg
    33361 accattgtct tcatgccgtt tacctattcg attgcagacg gcatcgcctt cggcttcatc
    33421 agctatgccg tggttaaact tttatgccgc cgcaccaaag acgttccgcc tatggtatgg
    33481 attgttgccg tattgtgggc actgaaattc tggtatttgg gctgattgat tcgatattaa
    33541 aaatgccgtc tgaaaggttt tcagacggca ttttgtttgc cgatatattt aatttttatt
    33601 aaattatata aaaatcaaat acataataaa atacatcgga ttgcttaaaa ataatacatt
    33661 gtttttatgt ataaaatatt ttataagttt tcaggatttt gattatcgaa aatttttctt
    33721 gatttcctga caattttatt gaaacaaata attcaaaatt aatctagttt aatcatggaa
    33781 ttaaaataaa atattaaaat tatgtaatga gtctccttaa aaatgtttga cattttcagt
    33841 cttgtgtttt agattatcga aaaataaaac tacataacac tacaaaggaa cattactatg
    33901 aaaccaattc agatgttttc cccttttctg aataatcccc ttgttttctt cttgtctgcg
    33961 gttttgccgc ataattccga acggtctgct gtttttcttt gattcgtttt aaatatcaat
    34021 aagataattt ttcccatata tttttaatga ttggattggg atgcccgacg cgtcggatgg
    34081 ctgtgttttg ccgtccgaat gtgatggaag cctgtccata ctgaaaaaaa gtctataaag
    34141 gagaaatatg atgagtcaac actctgccgg agcacgtttc cgccaagccg tgaaagaatc
    34201 gaatccgctt gccgtcgccg gttgcgtcaa tgcttatttt gcacgattgg ccacccaaag
    34261 cggtttcaaa gccatctatc tgtccggcgg cggcgtggca gcctgttctt gcggtatccc
    34321 tgatttgggc attaccacaa tggaagatgt gctgatcgac gcacgacgca ttacggacaa
    34381 cgtggatacg cctctgctgg tggacatcga tgtgggttgg ggcggtgcat tcaatattgc
    34441 ccgtaccatt cgcaactttg aacgcgccgg tgttgcagcg gttcacatcg aagatcaggt
    34501 agcgcaaaaa cgctgcggcc accgtccgaa caaagccatt gtatctaaag atgaaatggt
    34561 cgaccgtatc aaagctgccg tagatgcgcg cgttgatgag aacttcgtga ttatggcgcg
    34621 taccgatgcg ctggcggtag aaggtttgga tgccgctatc gaacgcgccc aagcttgtgt
    34681 cgaagccggt gcggacatga ttttccctga agccatgacc gatttgaaca tgtaccgcca
    34741 atttgcagat gcggtgaaag tgcccgtgtt ggcgaacatt accgagtttg gttccactcc
    34801 gctttatacc caaagcgagc tggctgaaaa cggcgtgtcg ctggtgctgt atccgctgtc
    34861 atcgttccgt gcagcaagca aagccgctct gaatgtttac gaagcgatta tgcgcgatgg
    34921 cactcaggcg gcggtggtgg acagtatgca aacccgtgcc gagctgtacg agcatctgaa
    34981 ctatcatgcc ttcgagcaaa aactggataa attgtttcaa aaatgattta ccgctttcag
    35041 acggtctttc aacaaatccg catcggtcgt ctgaaaaccc gaaacccata aaaacacaaa
    35101 ggagaaatac catgactgaa actactcaaa ccccgacctt caaacctaag aaatccgttg
    35161 cgctttcagg cgttgcggcc ggtaataccg ctttgtgtac cgttggccgt accggcaacg
    35221 atttgagcta tcgcggttac gacatcttgg atttggcaca aaaatgcgag tttgaagaag
    35281 tcgcccacct gctgattcac ggtcatctgc ccaacaaatt cgagctggcc gcttataaaa
    35341 ccaagctcaa atccatgcgc ggcctgccta tccgtgtgat taaagttttg gaaagcctgc
    35401 ctgcacatac ccatccgatg gacgtaatgc gtaccggcgt atccatgctg ggctgcgttc
    35461 atcctgaacg tgaaagccat ccggaaagtg aagcgcgcga catcgccgac aaactgatcg
    35521 ccagcctcgg cagcatcctc ttgtactggt atcaatattc gcacaacggc aaacgcattg
    35581 aggttgaaag cgacgaagag accatcggcg gtcatttcct gcaactgttg cacggcaaac
    35641 gcccaagcga atcccacatc aaagccatgc acgtttcact gattctgtat gccgaacacg
    35701 agttcaacgc ttctaccttc accgcccgcg tgattgccgg tacaggctct gatatgtact
    35761 ccagcattac cggagcaatc ggcgcgttga aaggtccgaa acacggcggc gcgaacgaag
    35821 tggcttacga tattcaaaaa cgctaccgca atgccgacga agccgaagca gacatccgcg
    35881 aacgcatcgg ccgcaaagaa atcgtgatcg gtttcggtca tccagtgtac accatttccg
    35941 accctcgcaa cgttgtcatt aaagaagtgg cacgcggttt gagcaaagaa accggcgata
    36001 tgcgcctctt tgacattgcc gaacgtttgg aaagcgtgat gtgggaagag aaaaaaatgt
    36061 tcccgaatct ggactggttc tctgccgttt cctaccaaaa attgggcgta ccgaccgcta
    36121 tgttcacacc gctgttcgta atttcccgta caaccggttg gagcgcacac gttcttgagc
    36181 aacgcaaaga cggcaaaatc atccgtccga gcgcgaacta cacaggccct gaagatttgg
    36241 cgtttgtgga gattgaagaa cgataattga agaatgcaat agcagtttgt tctttatttc
    36301 ggtatgcaaa gctaaggatt tcagacgacc ttgccttatt ggaaaggttg tctgaaataa
    36361 gtttaatcta ataggagaag ataatcctgt attggcgcaa gtaacaggat aagaaacatg
    36421 gaagatttat acataatact cgctttgggt ttggttgcaa tgattgccgg atttatcgat
    36481 gcgattgcgg gcgggggtgg tttgattacg ctgcccgcac tcttgttggc aggtattcct
    36541 cccgtgtcgg caattgccac caacaagctg caagcagccg ctgctacgtt ttcagctacg
    36601 gtttcttttg cacgcaaagg tttgattgat tggaagaaag gtctcccgat tgccgcagca
    36661 tcgtttgtag gcggcgtggc cggtgcatta tcggtcagct tggtttccaa agatattctg
    36721 ctggcggtcg tgccggtttt gttgatattt gtcgcactgt attttgtgtt ttcgcccaag
    36781 ctcgacggca gtaaggaagg caaagccaga atgtcttttt ttctgttcgg gctgacggtc
    36841 gcaccgcttt tgggttttta cgacggtgtg ttcggaccgg gtgtcggctc gttttttctg
    36901 attgccttta ttgttttgct cggctgcaag ctgttgaacg cgatgtctta caccaaattg
    36961 gcgaacgttg cctgcaatct tggttcgcta tcggtattca tgctgcacgg ttcaattatt
    37021 tttccgattg cggcaacgat ggcggccggt gcgtttgtcg gtgcgaattt aggtgcgaga
    37081 tttgccgtcc gcttcggttc gaagctgatt aagccgctgc tgattgtcat cagcatttcg
    37141 atggctgtga aattgttgat agacgagaga aatccgctgt atcagatgat tgtttcgatg
    37201 ttttaaaccc tttcagacga ccccttcgac aaacgtcggc tgaaacccca aaccacaaga
    37261 aaaatagatc cacaggagaa ctgaacatgg ctgccaacca acgttaccgc aaaccgctgc
    37321 ccggtacgga tttggaatac tacgacgcgc gtgcggcgtg tgaggacatc aagcccggct
    37381 cttacgacaa gctgccttac acgagccgca ttttggcgga gaatttggtc aaccgcgcgg
    37441 acaaagtcga tttgccgacg ctgcaaagct ggctgggtca gctgattgag ggaaaacagg
    37501 aaatcgactt tccttggtat ccggcgcggg tggtgtgcca cgatattctg gggcagaccg
    37561 cgttggtgga tttggcaggt ctgcgcgatg cgattgccga aaaaggcggc gatcctgcca
    37621 aagtgaatcc ggtggtgcaa acccagctca tcgtcgacca ctctttggcc gttgaatgcg
    37681 gcggctacga ccccgatgcc ttccgcaaaa accgccaaat cgaagacaga cgtaacgaag
    37741 accgtttcca cttcatcaac tggacgaaaa ccgcctttga aaatgtggac gtgattccgg
    37801 cgggcaacgg catcatgcac caaatcaatc tggaaaaaat gtcgcccgtc gtccaagtca
    37861 aaaacggcgt ggcgttcccc gatacctgcg tcggcacgga ttcgcacacg ccgcacgtcg
    37921 atgcgctggg cgtgatttcc gtgggcgtgg gcggattgga agcggaaacc gtaatgctgg
    37981 gacgcgcgtc catgatgcgc ctgcccgata ttgtcggtgt ggtgctgacc ggcaaacggc
    38041 aggcgggcat tacggcgacg gatattgtgt tggcactgac cgagtttctg cgcaaagaac
    38101 gcgtggtcgg ggcgtttgtc gaattcttcg gcgagggcgc gagaagcctg tctatcggcg
    38161 accgcgcgac catttccaac atgacgccgg agttcggcgc gactgccgcg atgttcgcta
    38221 ttgatgagca aaccattgat tatttgaaac tgaccggacg cgacgacgcg caggtgaaat
    38281 tggtggaaac ctacgccaaa accgcaggct tgtgggcaga tgccttgaaa accgccgttt
    38341 atcctcgcgt tttgaaattt gatttgagca gcgtaacgcg caatatggca ggtccaagta
    38401 acccgcatgc ccgttttgcc accgtcgatt tggcggcgaa agggctggcg aagccttacg
    38461 aagagccttc agacggacaa atgcccgacg gcgcggtcat catcgccgcg attaccagtt
    38521 gcaccaacac ttccaacccg cgcaacgtcg ttgccgccgc gctcttggcg cgcaacgcca
    38581 accgcttcgg gctgaaacgc aaaccgtggg tcaaaacctc gtttgccccc ggttcgaaag
    38641 tggcggaaat ttatttgaaa gaagcaggcc tgctgcccga aatggaaaaa ctcggcttcg
    38701 gtatcgtcgc cttcgcctgc accacctgca acggcatgag tggcgcgctg gatccgaaaa
    38761 tccagaaaga aatcatcgac cgcgatttgt acgccaccgc cgtattatca ggcaaccgca
    38821 acttcgacgg ccgtgtccat ccgtatgcga aacaggcttt cctcgcttcg cctccgttgg
    38881 tcgttgccta cgcgctggca ggcagtatcc gtttcgatat tgaaaacgac gtactcggcg
    38941 ttgcagacgg caaggaaatc cgcctgaaag acatttggcc tgccgatgaa gaaatcgatg
    39001 ccgtcgttgc cgaatatgtg aaaccgcagc agttccgcga tgtgtatgta ccgatgttcg
    39061 acaccggcac agcgcaaaaa gcacctagtc cgctgtacga ttggcgtccg atgtccacct
    39121 acatccgccg tccgccttac tgggaaggcg cgctggcagg ggaacgcaca ttaagaggta
    39181 tgcgtccgct ggcgattttg cccgacaaca tcaccaccga ccacctctcg ccgtccaatg
    39241 cgattttggc cgtcagtgcc gcaggcgagt atttggcgaa aatgggtttg cctgaagaag
    39301 acttcaactc ttacgcaacc caccgcggcg accacttgac cgcccaacgc gctaccttcg
    39361 ccaatccgaa actgtttaac gaaatggtga aaaacgaaga cggcagcgtg cgccaaggct
    39421 cgttcgcccg cgtcgaaccc gaaggcgaaa ccatgcgcat gtgggaagcc atcgaaacct
    39481 atatgaaccg caaacagccg ctcatcatca ttgccggtgc ggactatggt caaggctcaa
    39541 gccgcgactg ggctgcaaaa ggcgtacgcc tcgccggcgt agaagcgatt gttgccgaag
    39601 gcttcgagcg tatccaccgc accaacctta tcggcatggg cgtgttgccg ctgcagttca
    39661 aacccgacac caaccgccat accctgcaac tggacggtac ggaaacctac gacgtggtcg
    39721 gcgaacgcac accgcgctgc gacctgaccc tcgtgattca ccgtaaaaac ggcgaaaccg
    39781 ttgaagttcc cgttacctgc cgcctcgata ctgcagaaga agtattggta tatgaagccg
    39841 gcggcgtgtt gcaacggttt gcacaggatt ttttggaagg gaacgcggct tagaggtcgt
    39901 ctgaaaagca agacgtagcg tgggtcgggt tcaacatttt gctcattcac gtaattctcg
    39961 atatggcagg catctactgt aaatcgtcat tcccgcgcag gcgggaatcc agaaagtgga
    40021 attgaggaaa ccttatttat ccgatgagtt tctgtgcgga caaatctgga ttcccgcctg
    40081 cgcgggaatg acggggttta ataatctgcc gtatcacaac acagtagccg tagattgtgg
    40141 cgaaccccga cagtttgcgg aatcaaacgg ctttgtcgga gtggcagcct aatgtacttc
    40201 tggaaagtgg gtgtagcgtg ggctttgccc gcgaaataaa ggctgaattg acatggtata
    40261 gaggattaac aaaaatcggg acaaggcggc gaagccgcag acagtacaga tagtacggaa
    40321 ccgattcact tggtgcttca gcaccttaga gaatcgttct ctttgagcta aggcgagcca
    40381 acgctgtact ggtttttgtt aatccactat aaatttaatc cactatactg taaatcgtca
    40441 ttcccgcgca ggcgggaatc cagaaagtgg aattgaggaa acctttttat ccgatgagtt
    40501 tctgtgcgga taaatctgga ttcccgcctg cgcgggaatg acggggttta ataatctgcc
    40561 gtatcacaac acagtagccg tagattgtgg cgaaccccga cagtttgcgg aatcaaacgg
    40621 ctttggtcgg agtggcagcc taatccacta taaaaatcgt gggcagagcc cacgctacat
    40681 aaggagaatc tagaaatgcc gcaaattaaa atccccgccg tttactaccg cggtggcaca
    40741 tcaaaaggcg tgtttttcaa acgttccgat ctgcctgagg cggcgcggga agcgggtagc
    40801 gcgcgcgaca aaatcctgtt gcgcgtactc ggtagccccg acccttatgg taaacagatt
    40861 gacggtttgg gcaacgccag ttcgtccacc agcaaagccg tgattttgga caagtccgaa
    40921 cgcgccgatc acgatgtcga ttaccttttc gggcaagttt ccatcgacaa accttttgtc
    40981 gattggagtg gcaactgcgg caacctcacc gctgccgtgg gcgcattctc catcgagcaa
    41041 ggcttggtcg ataaaggcaa gattccttca gacggcatct gcacggtcaa aatctggcag
    41101 aaaaacatcg gcaaaaccat tattgcccat gtaccgatgc aaaacggcgc agttttggaa
    41161 acaggcgatt ttgagctcga cggcgtaacg ttcccggcag ccgaagtaca aatcgaattt
    41221 cttgatccag ccgacggcga aggcagtatg ttcccaaccg gcaatttggt cgatgaaatt
    41281 gatgtgccga atataggccg tttgaaagcc acgctcatca acgcgggcat tccgaccgtt
    41341 ttcctgaatg ccgccgactt gggctacacg ggcaaagagt tgcaagacga catcaacaac
    41401 gatgccgcag ctttggaaaa attcgagaaa atccgcgctt acggtgcgct gaaaatgggt
    41461 ctgatcagcg acgtatccga agctgccgcc cgcgcgcaca cgccgaaagt cgccttcgtc
    41521 gcgcccgccg ccgattacac cgcctccagt ggcaaaaccg tgaatgccgc cgacatcgat
    41581 ttgctggtac gcgccctgag catgggcaaa ttgcaccacg cgatgatggg taccgcctct
    41641 gttgccattg cgaccgccgc cgccgtgccc ggtacgctgg tcaaccttgc cgcaggcggc
    41701 ggaacgcgta aagaagtgcg cttcgggcat ccttccggca cattgcgcgt cggtgcagcc
    41761 gccgaatgtc aggacggaca atggacggcc accaaagcgg ttatgagccg cagcgcacgc
    41821 gtgatgatgg aaggttgggt cagggtgccg gaagattgtt tttaaattga cgtagcatgg
    41881 gtttgcccgc gagccataaa caggtcgtct gaaaaacaag taaacatcaa atcactgacc
    41941 attcctttcc cttgccctgt ggcggaaggc ggcaaatcac aaggaagaac acggaaaccc
    42001 cgataaaaga cagcttcccg tattaccgtc attcccgcgc aggcgggaat ctagacctgt
    42061 caatatggag gattggtagg ggaaaacagg tttcgtgagt tctacattct ggattcccgc
    42121 cacagcctgt cctcgcgtag gcggggacgg aataacgata gaaaatgcgg catacgcttt
    42181 gcccaaagag gccgtctgaa acaccttgcg cctgatgtct gcctttttca gacgacccca
    42241 caccaaaaaa acaaccacaa actacaagga gaaacatcat gtccgaccaa ctcatcctcg
    42301 ttctgaactg cggcagttca tcgctcaaag gcgccgttat cgaccgaaaa agcggcagcg
    42361 tcgtcctaag ctgcctcggc gaacgcctga ccacgcccga agccgtcatt acgttcaaca
    42421 aagacggcaa caaacgccaa gttcccctga gcggccgaaa ttgccacgcc ggcgcggtgg
    42481 gtatgctttt gaacgaactg gaaaaacacg gtctgcacga ccgcatcaaa gccatcggcc
    42541 accgcatcgc ccacggcggc gaaaaataca gcgagtctgt tttgatcgac caggccgtaa
    42601 tggacgaact caatgcctgc attccgcttg cgccgctgca caaccccgcc aacatcagcg
    42661 gcatccttgc cgcacaggaa catttccccg gtctgcccaa tgtcggcgtg atggatactt
    42721 cgttccacca aaccatgccg gagcgtgcct acacttatgc cgtgccgcgc gagttgcgta
    42781 aaaaatacgc tttccgccgc tacggtttcc acggcaccag tatgcgttac gttgcccctg
    42841 aagccgcacg catcttgggc aaacctctgg aagacatccg catgattatt gcccacttag
    42901 gcaacggcgc atccattacc gccatcaaaa acggcaaatc cgtcgatacc agtatgggtt
    42961 tcacgccgat cgaaggtttg gtaatgggta cacgttgcgg cgacatcgat ccgggcgtat
    43021 acagctatct gacttcccac gccgggatgg atgttgccca agtggatgaa atgctgaaca
    43081 aaaaatcagg tttgctcggt atttccgaac tttccaacga ctgccgcacc ctcgaaatcg
    43141 ccgccgacga aggccacgaa ggcgcgcgcc tcgccctcga agtcatgacc taccgcctcg
    43201 ccaaatacat cgcttcgatg gctgtggcct gcggcggcgt tgacgcactc gtgttcaccg
    43261 gcggtattgg cgaaaactca cgtaatatcc gagctaaaac cgtttcctat cttgatttct
    43321 tgggtctgca catcgacact aaagccaata tggacaaacg ctacggcaat tcgggtatta
    43381 tcagcccgac cgattcttct ccggctgttt tggttgtccc gaccaatgaa gaactgatga
    43441 ttgcctgcga cactgccgaa cttgtcggca tcttgtagcc aaaaaaggga cgagtccgca
    43501 caatgccgtc tgaaacccca aacgcccgat taggctgatg aggattttag acggcattgt
    43561 tcattttttt gttatcttgc atttttgtgc ggacggtgga atttcatcct gtaaacataa
    43621 atatttgtcg gaaaacagaa accctccgcc gccatttcta cgaaagcagg aaaccagcaa
    43681 cgcaaagcga cagggatttg ttggaaatga ccgaaaccga acgaaccgga ttcccgcctg
    43741 cgcgggaatg acgggatttt ctgtttttgt ggaaatgacg ggattttgaa tttcgggcgt
    43801 acaatacgga aaacatgacg ataaggaaaa aaactatggc acagtttttc gctattcatc
    43861 ccgacaaccc ccaagaacgc ctcatcaagc aggcggtcga aatcgtcaat aaaggcggcg
    43921 tggtcgttta tccgaccgat tcctgttatg ccttgggttg caaactcggc gataaggcgg
    43981 cgatggaacg catactctcc atccgaaaaa tcgatttgaa acaccacctg accctgatgt
    44041 gcgccgattt gagcgagttg ggcacatacg ccaaaatcga caacgtacag tttcgtcagc
    44101 ttaaggccgc cacgcccggg ccttatacct ttatcttgca ggcgacgaag gaagtgccgg
    44161 cgcgcacgct gcacccgaaa cgcaaaacca tcgggctgcg tattcccgat aatgccattg
    44221 cacaagccct gctgggggaa ttgggcgagc cgcttttaag ctgcaccctg atgctgcccg
    44281 aagacggcga accattgacc gatccttatg aaatccgcga gcgtttggaa cacgccgtcg
    44341 atttggtgat tgacggcggc tggtgcggaa ccgagccgac caccgtcgtc gatatgaccg
    44401 acggcacgga actggtgcgc cggggttgtg gcgatacggc ggtgttcggt ttgtagggaa
    44461 accgatgccg tctgaagcat cggctgttca gacggcattg cgcgccttgc cggcggcagt
    44521 ccgaaatgcc ggcgcgtatc gcgctcggtc ggaatatccg tttgaaacgg cattttgatg
    44581 cattactgca ccgcaatcgg aattctcggt tcgtagagca ggtcgtaggt cggcttgttg
    44641 agcaggtctt ggagcgtgaa accgtccaga tacgtgaaaa acgacttcat tgcaccgccg
    44701 agtatgcctg tcagacggca ggacggtgta atcaggcatt cgttgttcga acccatacat
    44761 tcgaccagct gcatcggttc gaggtggcgg acgaccgagc cgatgttgat gcggtcgggc
    44821 ggtgtggcaa gccgcagacc gccgcctttt ccgcgcacgc tgtggaggaa gccgcctttg
    44881 accagcgcgg taacgacctt catcagatgg cttttggaaa tgccgtaggt tacggcgatg
    44941 gtactgatgt tgaccagcgc atcgtcgttg atggcagtgt agataaggac gcgcagcccg
    45001 tagtccgtat gttgtgtcaa atacatgatt ttctcggtat ggattgttat tcttatcggt
    45061 acggtttaag gttcacggac aataccttaa tggttgaaac cctgtccgtc ggggcggtag
    45121 aatgcagcct gtctgcggcg gtatgccgtc tgaaacatcc gcgctaccgt ttgagaattt
    45181 gttattgtaa ctcaaaatca tgaaaccgtt gaaacgacat cccgccctta tcgggctttc
    45241 gcgtgaccac caccattcgc tttccctgtg cgtgcgtctg ttgcggacgc cggaagaaag
    45301 gcatcgggac gaactcgaac cgcatttttc cgaattggaa acccattttc gcgaagaaga
    45361 aaccaagttt gccccaattt ggcagaatgt cgcccccgaa ttgaaacaac gtttcgagaa
    45421 agaccacgcc cgactgcggc agatgatggc aagccccgaa tacggtaacg cggcgtggaa
    45481 taccgctttt gccacaaccc tgcgcgacca cgcgcgcttt gaagaacgcg agctgtttcc
    45541 cgccgccgaa ccgtttttgc cggcatgatt ccgttttgcg gcaaatatat taatgataaa
    45601 caaggaacac acatgaaatt taccaagcac cccgtctggg caatggcgtt ccgcccgttt
    45661 tattcgctgg cggctctgta cggcgcattg tccgtattgc tgtggggttt cggctacacg
    45721 ggaacgcacg agctgtccgg tttctattgg cacgcgcatg agatgatttg gggttatgcc
    45781 ggactggtcg tcgtcgcctt cctgctgacc gccgtcgcca cttggacggg gcagccgccc
    45841 acgcggggcg gcgttctggt cggcttgact atcttttggc tggctgcgcg gattgccgcc
    45901 tttatcccgg gttggggtgc gtcggcaagc ggcatactcg gtacgctgtt tttctggtac
    45961 ggcgcggtgt gcatggcttt gcccgttatc cgttcacaga atcaacgcaa ctatgttgcc
    46021 gtgttcgcgc tgttcgtctt gggcggcacg catgcggcgt tccacgtcca gctgcacaac
    46081 ggcaacctag gcggactctt gagcggattg cagtcgggct tggtgatggt gtcgggtttt
    46141 atcggtctga ttggtacgcg gattatttcg ttttttacgt ccaaacgctt gaatgtgccg
    46201 cagattccca gtccgaaatg ggtggcgcag gcttcgctgt ggctgcccat gctgactgcc
    46261 atgctgatgg cgcacggtgt gttggcttgg ctgtctgccg tttttgcctt tgcggcaggt
    46321 gtgattttta ccgtgcaggt gtaccgctgg tggtataaac ccgtgttgaa agagccgatg
    46381 ctgtggattc tgtttgccgg ctatctgttt accggattgg ggctgattgc ggtcggcgcg
    46441 tcttatttca aacccgcttt cctcaatctg ggtgtgcatc tgatcggggt cggcggtatc
    46501 ggcgtgctga ctttgggcat gatggcgcgt accgcgcttg gtcatacggg caatccgatt
    46561 tatccgccgc ccaaagccgt tcccgttgcg ttttggctga tgatggcggc aaccgccgtc
    46621 cgtatggttg ccgtattttc ttccggcact gcctacacgc acagcatccg cacctcttcg
    46681 gttttgtttg cactcgcgct tttggtgtat gcgtggaagt atattccttg gctgattcgt
    46741 ccgcgttcgg acggcaggcc cggttgagac aaaccgccgc agatttcggg tctgggcggt
    46801 ttgcttttca gacggcaggg cggtcagttg ccgtccagcc agcggtcgcg tgtggttttg
    46861 gcttcttcaa aatagcggta cagggcttcg cggtcgtcgg tggtcaggat gtttgccaaa
    46921 acgtccaact gtttgcccaa gccttgaacc agttgcagca ggctgtcttt gttggcaagg
    46981 cagatgtccg cccacacggc gggatgaccg gaggcgatgc gggtgaagtc ccgaaagccc
    47041 gtggcggcga atttcagata ttcctgtccg tcggggtggt cgagaatctg gtggacatag
    47101 gcgaaggcgg tcaggtgggg catatgggag acggcggcga aaaccgcgtc gtggcgttgc
    47161 gcgtccatcg tataaatttc cgcaccgacc gcgtgccaca ggttttctac caaggcaatg
    47221 ccgtctgaat gttcgccgcc gtgtggcgtg atgatgagtt ttctgtggcg gaacagcccg
    47281 aactgcgcgg cttgcgcacc gcttctgtcc gaaccggcaa tcgggtgggc ggcgatgcag
    47341 cggtgcaggc ggtcgggcag acagcggcgg aaggcttcga tgaccgaaga tttggtgctg
    47401 ccgacatcgg aaatccaagt gtgttccggc aaaacggggc gcagcgcggt caaaatggca
    47461 ggaacggtgg cgacgggtgt ggcaatcagt accaagtccg caccgccgat gctgtccgcg
    47521 tcgatggcaa cggaagcccg gtcaatcacg ccgcgttcca atgcacgttc gaggttgtcg
    47581 cggtcggtgt cgataccggt aacggtgcgg acgagtccct gccttttgag gtcgagaacg
    47641 aacgaaccgc cgatcagccc tacaccgatg agggcaatat ggttcaaaat gggcatttgt
    47701 gtaaacggtt ttcgcaaagt accgtcatgg tagcctatcg gcggaatatg ccgcaaggtc
    47761 ggcaggaaaa aggagaagaa atggacaaaa tcagagttgc cgccgtgcag atggtgtcgg
    47821 gcgtgtcgcc ggaaaccaac gtcgccgcca tgaaacgcct ggtcgcacgg gcggcggagc
    47881 agggtgcgga ttgggtgctg ctgcccgaat attgggtgct gatgggcgca aacgataccg
    47941 acaaactcgc gcttgccgag cctttgggcg gcggacgctt tcagacggca ttgagcgaaa
    48001 cggcgaaaga atgcggcgtg gtgctgttcg gcggaaccgt gccgctgcaa agccccgagg
    48061 cgggcaaggt catgaatact ttattggtgt acgggcgcga cggcaaacgg acggggctgt
    48121 accacaaaat gcacctcttc ggcttttccg gtttgggcga acgctatgcc gaagccgata
    48181 ccatcagcgc agggcgggaa gtgccgcact tgtcggcaga aggcgtgtcg gtggcggcgg
    48241 gcatttgtta cgatgtccgc tttcccgaat ttttccgccg ccagctgccg tttgacgtat
    48301 tgatgctgcc cgctgcgttt acgcacacga cgggcaaggc gcattgggag ctgctgctgc
    48361 gcgcgcgtgc cgtcgaaaac caatgttacg tcgtggcggc ggcacagggc ggtttgcacg
    48421 aaaacggacg gcgcacgttc ggacacagca tgattgtcga tccgtggggc gacgtattgg
    48481 acgtattacc cgaaggcgaa ggcatcgtta cggcagacat cgatgccaac cgcctgaaca
    48541 gcgtccgcaa ccgcctgccc gccttgaaat accgggtttt ggatgccgtc tgaaggttca
    48601 gacggcatcg gtgccgggga atcagcgggc tgtaggatgg gcttcagcct gccatttcca
    48661 tcaatcccac atttctaccg ttttcatcga atccatcgaa tccgcccctt tcgacgaccc
    48721 agtcctacgc aactgaaccg tcattcccac ggaagtggga atctagaatc tcgggggttt
    48781 cagtcatttc cgatagattc ccgccgcgtc aggggtctgg attcccgcct gcgcgggaat
    48841 gacgaatcca tccatacgga aacctgcacc acgtcattcc cacggaagtg ggaatctaga
    48901 atctcggggt ttcagtcatt tccgatagat tcccgccgcg tcgggggtct ggattcccgc
    48961 ctgcgcg
//
LOCUS       DAWFLT010000005        46427 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWFLT010000005 DAWFLT010000000
VERSION     DAWFLT010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 46427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46427
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.005"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..251)
                     /locus_tag="WGR74_000251"
     CDS             complement(<1..251)
                     /locus_tag="WGR74_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain protein"
                     /protein_id="HEZ0904752.1"
                     /translation="MEDKQGMTKAVVGVMTDALADGRKPATASNLPPLSNRRGTETET
                     AGRVQEVFECYEMYITDGKGNLLGVPLRRGVSDSAFIDQ"
     gene            354..569
                     /locus_tag="WGR74_000252"
                     /pseudo
     CDS             354..569
                     /locus_tag="WGR74_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(721..1779)
                     /gene="trpD"
                     /locus_tag="WGR74_000253"
     CDS             complement(721..1779)
                     /gene="trpD"
                     /locus_tag="WGR74_000253"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ0904753.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSISQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(1843..2433)
                     /locus_tag="WGR74_000254"
     CDS             complement(1843..2433)
                     /locus_tag="WGR74_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ0904754.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIDRNTMPECLEVTAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            2853..5129
                     /locus_tag="WGR74_000255"
     CDS             2853..5129
                     /locus_tag="WGR74_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0904755.1"
                     /translation="MAQTTLKPIVLSILLISTPILAQAHETEQSVDLDEVTVVGKSRP
                     RATSGLLHTSTASDKILSGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDDAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSAISEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIQYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGFHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            5813..5920
                     /locus_tag="WGR74_000256"
                     /pseudo
     CDS             5813..5920
                     /locus_tag="WGR74_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(6065..6862)
                     /locus_tag="WGR74_000257"
     CDS             complement(6065..6862)
                     /locus_tag="WGR74_000257"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ0904756.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QIESEPESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
     gene            7017..9866
                     /gene="uvrA"
                     /locus_tag="WGR74_000258"
     CDS             7017..9866
                     /gene="uvrA"
                     /locus_tag="WGR74_000258"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ0904757.1"
                     /translation="MYKHHPQHSHDNDTIRIRGARTHNLKNVDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVCVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDSEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCEQSITGQYLSSKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGIPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIACGSPEQVAKVKGSYTGKYLKVVLR"
     gene            9900..11495
                     /locus_tag="WGR74_000259"
     CDS             9900..11495
                     /locus_tag="WGR74_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="funZ protein"
                     /protein_id="HEZ0904758.1"
                     /translation="MKPLKTLEFGFVDAANYRRRENKDLFNRIFVKGEYLDELCEPNI
                     SFLIGEKGTGKTAYAVYLTNNFYKNIHATTKFVRETDYSKFIQLKKARHLTVSDFTSI
                     WKVILYLLISNQIKCKENGILSSIFNKFKALDEAINEYYYGAFDPEIVQAITLIENSK
                     EAAEMIFGKFVKLGEEESQQITFTESKFQANLGFIERKFKDALSQLKLKDNHILFIDG
                     IDIRPSQIPFDEYHECVKGLANAIWMLNNDIFPSIKDSKGRMRVVLLIRPDIFDSLGL
                     QNQNTKLQDNSVFLDWRTDYKSYRSSKIFGVFDHLLRTQQEKQDSLEKGNSWDYYFPW
                     NAPNLHDEYKNLTSFISFLRKSYYRPRDILQMLTLLQKNKKSKEDYVVAEDFDNTSFQ
                     REYSIYLLGEIKDHLLFYYSQSDYQNFLKFFEFLNGKDRFKYSDFLKAFERLKKHLQT
                     TSVEIPKFMSTANEFLQFLFDLNVIAYLDNPEDETKPYIHWCFKDRNYANISPKIKTE
                     TEYLIFSGLSKALDVGTPFKNKQ"
     gene            complement(11802..12017)
                     /locus_tag="WGR74_000260"
     CDS             complement(11802..12017)
                     /locus_tag="WGR74_000260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904759.1"
                     /translation="MPSETGNRVSDGILFVISKRGSLNIPTLFFPFWGSQRPLQNSPK
                     SPKFPPRHLGDFGEFCKGLGLSVPVCA"
     gene            complement(12046..12936)
                     /gene="sucD"
                     /locus_tag="WGR74_000261"
     CDS             complement(12046..12936)
                     /gene="sucD"
                     /locus_tag="WGR74_000261"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ0904760.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(12947..14113)
                     /gene="sucC"
                     /locus_tag="WGR74_000262"
     CDS             complement(12947..14113)
                     /gene="sucC"
                     /locus_tag="WGR74_000262"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ0904761.1"
                     /translation="MNLHEYQAKELLASYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            complement(14212..14499)
                     /locus_tag="WGR74_000263"
     CDS             complement(14212..14499)
                     /locus_tag="WGR74_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904762.1"
                     /translation="MNKEIVGIFFIPAGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            complement(14594..16027)
                     /gene="lpdA"
                     /locus_tag="WGR74_000264"
     CDS             complement(14594..16027)
                     /gene="lpdA"
                     /locus_tag="WGR74_000264"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0904763.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDVKFDVAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            16224..16340
                     /locus_tag="WGR74_000265"
                     /pseudo
     CDS             16224..16340
                     /locus_tag="WGR74_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(16392..17573)
                     /gene="odhB"
                     /locus_tag="WGR74_000266"
     CDS             complement(16392..17573)
                     /gene="odhB"
                     /locus_tag="WGR74_000266"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ0904764.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAAPAEAAPAAAPAA
                     AQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAAAAAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLLD
                     L"
     gene            complement(17673..20501)
                     /locus_tag="WGR74_000267"
     CDS             complement(17673..20501)
                     /locus_tag="WGR74_000267"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ0904765.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMTLQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SVRAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADAYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQPIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVVGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(20706..21989)
                     /gene="gltA"
                     /locus_tag="WGR74_000268"
     CDS             complement(20706..21989)
                     /gene="gltA"
                     /locus_tag="WGR74_000268"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0904766.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(22101..22349)
                     /locus_tag="WGR74_000269"
     CDS             complement(22101..22349)
                     /locus_tag="WGR74_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ0904767.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(22353..23060)
                     /locus_tag="WGR74_000270"
     CDS             complement(22353..23060)
                     /locus_tag="WGR74_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ0904768.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(23181..24944)
                     /gene="sdhA"
                     /locus_tag="WGR74_000271"
     CDS             complement(23181..24944)
                     /gene="sdhA"
                     /locus_tag="WGR74_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ0904769.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(24947..25288)
                     /gene="sdhD"
                     /locus_tag="WGR74_000272"
     CDS             complement(24947..25288)
                     /gene="sdhD"
                     /locus_tag="WGR74_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ0904770.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(25282..25659)
                     /gene="sdhC"
                     /locus_tag="WGR74_000273"
     CDS             complement(25282..25659)
                     /gene="sdhC"
                     /locus_tag="WGR74_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ0904771.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(25938..27341)
                     /locus_tag="WGR74_000274"
     CDS             complement(25938..27341)
                     /locus_tag="WGR74_000274"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0904772.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(27590..28327)
                     /locus_tag="WGR74_000275"
     CDS             complement(27590..28327)
                     /locus_tag="WGR74_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ0904773.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEELGDPFKVSDADTM
                     LQFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(28606..30882)
                     /gene="metE"
                     /locus_tag="WGR74_000276"
     CDS             complement(28606..30882)
                     /gene="metE"
                     /locus_tag="WGR74_000276"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ0904774.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADYVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTELAAAGAEWIQIDEPALAVDLPQAWL
                     DAYQDVYATLSQVNAKILLSTYFGSVAAHAGRLKALPVAGLHIDLVRAPEQLDAFADY
                     GKVLSAGVIDGRNIWRANLNNVLATLEPLKAKLGDRLWISSSCSLLHTPYDLSVEEKL
                     KVNKPALFSWLAFTLQKTQELRVLKTALNQGREAVAAELADSQAAADSRANSTEIHRA
                     EVAKRLADLPADADQRKSPFAERIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSDADYEAAMKKEIALVIEEQEKLNLDVLVHGEAERNDMVEYFGELLEGFAFTQY
                     GWVQSYGSRCVKPPVIYGDVSRPNAMTVAWSTYAQTLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLAWAS
                     EAFRLSSTGCEDSTQIHTHMCYSEFNDILPAIASMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPFERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(31020..31898)
                     /gene="metF"
                     /locus_tag="WGR74_000277"
     CDS             complement(31020..31898)
                     /gene="metF"
                     /locus_tag="WGR74_000277"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ0904775.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            32099..32374
                     /locus_tag="WGR74_000278"
     CDS             32099..32374
                     /locus_tag="WGR74_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ0904776.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            32374..32499
                     /gene="ykgO"
                     /locus_tag="WGR74_000279"
     CDS             32374..32499
                     /gene="ykgO"
                     /locus_tag="WGR74_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ0904777.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRKGKVYVICKSNPRFKARQR"
     gene            complement(32607..32807)
                     /locus_tag="WGR74_000280"
     CDS             complement(32607..32807)
                     /locus_tag="WGR74_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904778.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRALREVAEISAWVLLG
                     AAAAMLFWYLFMLYFKHIPDSY"
     gene            32957..34096
                     /locus_tag="WGR74_000281"
     CDS             32957..34096
                     /locus_tag="WGR74_000281"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ0904779.1"
                     /translation="MSQNASVGIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALADYLGIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            34093..34674
                     /gene="metW"
                     /locus_tag="WGR74_000282"
     CDS             34093..34674
                     /gene="metW"
                     /locus_tag="WGR74_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ0904780.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            34791..35636
                     /locus_tag="WGR74_000283"
     CDS             34791..35636
                     /locus_tag="WGR74_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ0904781.1"
                     /translation="MKNKTSSLLLWLAAMMLTACSPSKEDKTKENGASAASSTASAAS
                     SSAPQTDLQPAASAPDNVKQAESAPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(35686..36246)
                     /gene="efp"
                     /locus_tag="WGR74_000284"
     CDS             complement(35686..36246)
                     /gene="efp"
                     /locus_tag="WGR74_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ0904782.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(36289..37437)
                     /gene="earP"
                     /locus_tag="WGR74_000285"
     CDS             complement(36289..37437)
                     /gene="earP"
                     /locus_tag="WGR74_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ0904783.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDSEALRQRLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETVSAHRRLSDDLNGGGALSAT
                     QRLECWQILQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(37456..37734)
                     /locus_tag="WGR74_000286"
     CDS             complement(37456..37734)
                     /locus_tag="WGR74_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904784.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIILLIVYLFHYFGAF"
     gene            38236..39177
                     /gene="miaA"
                     /locus_tag="WGR74_000287"
     CDS             38236..39177
                     /gene="miaA"
                     /locus_tag="WGR74_000287"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ0904785.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLIGEITARGKYPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQQVDPETACRLKPNDSQ
                     RIGRALEVYYLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
     gene            39181..39900
                     /locus_tag="WGR74_000288"
     CDS             39181..39900
                     /locus_tag="WGR74_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family DNA transformation protein"
                     /protein_id="HEZ0904786.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTLPQEITQHDKTRLFAELKNHPPVAAFPPQEEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALSQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            39905..40393
                     /locus_tag="WGR74_000289"
     CDS             39905..40393
                     /locus_tag="WGR74_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ0904787.1"
                     /translation="MTVLTVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIERRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            40460..41212
                     /gene="rlmB"
                     /locus_tag="WGR74_000290"
     CDS             40460..41212
                     /gene="rlmB"
                     /locus_tag="WGR74_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ0904788.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYIQEGKSDARTREVLEK
                     AANENIRVYFVDADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMSGESDLYHCNLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKV"
     gene            complement(41274..42665)
                     /locus_tag="WGR74_000291"
     CDS             complement(41274..42665)
                     /locus_tag="WGR74_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ0904789.1"
                     /translation="MSGQLGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            43125..44000
                     /locus_tag="WGR74_000292"
     CDS             43125..44000
                     /locus_tag="WGR74_000292"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ0904790.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNNRLIPIYDTMFCEPSPAAPKWAVSALVRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            44009..45205
                     /gene="bamC"
                     /locus_tag="WGR74_000293"
     CDS             44009..45205
                     /gene="bamC"
                     /locus_tag="WGR74_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ0904791.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTMWQPSASDPNLEAAFLTRFMQYLGVDGQQAENASAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(45262..46194)
                     /gene="pip"
                     /locus_tag="WGR74_000294"
     CDS             complement(45262..46194)
                     /gene="pip"
                     /locus_tag="WGR74_000294"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ0904792.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETVWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIRHIPTVIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLADALVQAVEDIFPRLL"
BASE COUNT        11771 a        12519 c        11175 g        10962 t
ORIGIN      
        1 atttggtcga tgaaagccga atcggataca ccgcgcctaa gaggaacgcc taacaggttt
       61 cctttaccgt ccgttatgta catttcgtaa cattcgaaga cttcctgaac cctgcctgcc
      121 gtttcggttt ctgtacccct cctgttagat aaggggggaa gatttgaagc ggttgccggc
      181 ttcctgccgt ccgctaacgc gtccgtcatc acgccgacaa ccgcctttgt catcccttgc
      241 ttatcttcca tggtgcgaat cctcaaaaac aggcaaaaaa aaagccctgt tacttgtaga
      301 aagtaaagga cgttaatttt tgttaatcgt cccttcttag ggacgcaata tataaggttt
      361 ataccgtcgt tgttcctaat gcgcaatcag cgacattgtt gccaattatc cgaaagaaag
      421 ttaagcctga tggcattgta tacacggata cctttcgtag ttatgatgtg cttgatatta
      481 gtgaatttag ccatttacgt aagtttaacg gcattcccaa agagcatttg gggctgcatt
      541 taaagaaatg ccaatggcat tttaaataga atctcaaatt cagattttaa gacaattagt
      601 taatgggaat ttggtctggt tatctagtac agccccaagt ttttatataa aaacagtttg
      661 gtaagttctc ttcgtgaagt gcttatgttt tgccacaaaa gttccataat catggcggca
      721 tcacccaagc tccatctttc caagaaaatg gcatctttgt tgtggttgaa aaccgacaaa
      781 ctcctctttt ttcgatttgg ccctgcctga atcgatggct tcccgtgcgg cagatatgcc
      841 gtctgaaagc gaagcagcga catttccggc atatagggcg gcggcggtat taagcaatac
      901 gatatcgcgc gcagcccctt ctttcccatc cagcacctca ttcattttca acaaagattc
      961 ctgagtattg gcaactttga tttcatccaa attgcggcgg gtttcgatac cgaaatcttc
     1021 tgggcggatg tcgtattcgc tgatttttcc gtctttgagt tcggcaacgc gcgttttgcc
     1081 cgtcagtgta atttcatcca aaccgccctc cccgcaaacg actaaaacgt gttttgaacc
     1141 gagttgttgc aagacccgcg acaaaatgcc gcacaaatcg gtgtggaaca cgcccaaaag
     1201 ctggttcggc gcgcccgcag gattggtcaa cggacccaat atgttgaaaa tgcttcggaa
     1261 accgagcgaa cggcgtaccg gggcgacatg gcgcatggca ctgtggtgat tgggggcgaa
     1321 cataaaccct ataccggtct ggctgatact ttgggcaatc tgttcgggag tcaggttgag
     1381 gtttgcgccc atctgctcca tcacgtccgc cgcgccgctg gaagatgaaa cggaacgccc
     1441 gccgtgtttg gcaaccttcg cgcctgccgc tgcggcaaca aacatcgaag tcgtcgaaat
     1501 attgaaggtt ttggcaccat ccccgcccgt accgacgata tcaaccagcc cctccgcatt
     1561 ctccagcggc acttttgtcg caaactcgcg catgacggct gcagctgcgg taatttcgga
     1621 aacggtttca accttgatac gcaatcctgt caaaatcgcc gcaatttgtt cgggcggcac
     1681 ttttccgctc atcatttggc gcatcaagtc ggtcatttca tcgtaaaaca actcattatt
     1741 gctgattaat cgttcgatag cctgttgcgg tgtaatcatt ttttgtcctc cgttcaatat
     1801 tcggacgaaa atgccgtctg aagggcttca gacggcatca cgtcagattt tttgcggttt
     1861 gaagttttga aattcgatta aaaaattgtt taacatatca tgtccgtgct cggtcaagag
     1921 ggcttcgggg tggaactgca cgccctcgac ggcatattcc ttatggcgca cacccataat
     1981 ctcgccgtcc tcagtccaag ccgttacttc caaacattcg ggcatcgtat tccgatcgat
     2041 aacgaggctg tgataacgcg tacaggtaac cggattgggc aaacccttaa acataccctt
     2101 gcccgaatgg gacacgggcg acaccttacc gtgcatcagc gttttggcgc ggactatcct
     2161 gccgccgaac gcctcgccta tcgtctgatg cccgaggcac acgcccataa tcggcagccg
     2221 gccggcaaaa tggcgcatcg ccgccacgga aatccccgct tctttgggcg aacacgggcc
     2281 gggtccgata acgagatatt gcggattcaa tgcctcgatt tcttccaacg taatatcatc
     2341 gttgcggcgc acggcgactt cctgccccaa ttcagcgaaa tactggacga tgttgtaagt
     2401 aaaactgtcg taattgtcga taaacaaaag cattttgtga ttaacctatt gatttataat
     2461 ttgtttgtta ttaactgcat ttccgatacc accatccata ccccttatat cttagcgtgc
     2521 ccgatgcgcc ctcgtgaacc tgggcagagc ctcgcgttct gacaagtacg ccattttgcc
     2581 accttttcga ccgttcggga acgtgccgtt tttatgcact cataaatttt ggttatcccg
     2641 cagccgaaca tagtgggcaa acacagcgga atctgcgtca acatcaaccg cgcccccgaa
     2701 tgacggcgga tacaaatgga caccgcccga tgccgcaacc gtttcatttg aaaaactttt
     2761 gcctatgccc tctgagtgtt ctcaaaatct tatgatataa aaaatgtaat gttatataat
     2821 aacaaacttt tgaaatgcga cgagaagcca aaatggcaca aactacactc aaacccattg
     2881 ttttatcaat tcttttaatc agcaccccca tcctcgccca agcgcatgaa accgagcagt
     2941 ctgttgactt ggacgaggtt accgtcgtcg gcaaaagccg tccgcgcgcc acatccgggc
     3001 tgttgcacac ttcgaccgct tccgacaaaa tcctctccgg cgataccttg cgccaaaaag
     3061 ccgtcaactt gggcgatgct ttggacggcg tgccgggcat tcacgcctcg caatacggcg
     3121 gcggcgcgtc cgctcccgtt attcgcggtc aaacaggcag gcggattaaa gtgttgaacc
     3181 atcacggcga aacgggcgat atggcggatt tttcgcccga tcacgccatt atggtagata
     3241 ccgccttgtc gcaacaggtc gaaatcctgc gcgggccggt tacgctcttg tacagctcgg
     3301 gcaatgtggc ggggctggtc gatgttgccg atggcaaaat ccccgaaaaa atgcctgaaa
     3361 acggcgtatc gggcgaactc ggattgcgtt tgagcagcgg caatctggaa aaactcacgt
     3421 ccggcggcat caatatcggt ttgggcaaaa actttgtatt gcacacggaa gggctgtacc
     3481 gcaaatcggg ggattacgcc gtaccgcgtt accgcaatct gaaacgcctg cccgacagcc
     3541 acgccgattc gcaaacgggc agcatcgggc tgtcttgggt tggcgaaaaa ggttttatcg
     3601 gcgtagcgta cagcgaccgt cgcgaccaat atggtctgcc tgcccacagc cacgaatacg
     3661 atgattgcca cgccgacatc atctggcaaa agagcttgat taacaaacgc tatttacagc
     3721 tttatccgca cctgttgacc gaagaagaca tcgattacga caatccgggc ttgagctgcg
     3781 gcttccacga cgacgatgat gcacacgcac acacccacag cggcagaccg tggatagacc
     3841 tgcgcaacaa acgctacgaa ctccgtgccg aatggaagca accgttcccc ggttttgaag
     3901 ccctgcgcgt acacctgaac cgcaacgact accgccacga cgaaaaagca ggcgatgcag
     3961 tcgaaaactt ttttaacaac caaacgcaaa acgcccgcat cgagttgcgc caccaaccca
     4021 taggtcgtct gaaaggcagc tggggcgtgc aatatttaca acaaaaatcc agtgctttat
     4081 ctgccatatc cgaagcggtt aaacaaccga tgctgcttga caacaaagtg caacattaca
     4141 gctttttcgg tgtagaacag gcaaactggg acaacttcac gcttgaaggc ggcgtacgcg
     4201 tggaaaaaca aaaagcctcc attcagtacg acaaagcatt gattgatcgg gaaaactact
     4261 acaaccaccc cctgcccgac ctcggcgcgc accgccaaac cgcccgctca ttcgcacttt
     4321 cgggcaactg gtatttcacg ccacaacaca aactcagcct gaccgcctcc catcaggaac
     4381 gcctgccgtc aacgcaagag ctgtacgcac acggcaaaca cgtcgccacc aacacctttg
     4441 aagtcggcaa caaacacctc aacaaagagc gttccaacaa tatcgaactc gcgctgggct
     4501 acgaaggcga ccgctggcaa tacaatctgg cactctaccg caaccgcttc ggtaactaca
     4561 tttacgccca aaccttaaac gacggacgcg gccccaaatc catcgaagac gacagcgaaa
     4621 tgaagctcgt gcgctacaac caatccggcg ccgacttcta cggcgcggaa ggcgaaatct
     4681 acttcaaacc gacaccgcgc taccgcatcg gcgtttccgg cgactatgta cgaggccgtc
     4741 tgaaaaacct gccttcccta cccggcagag aagatgccta cggcaaccgt cctttcatcg
     4801 cacaggacga ccaaaatgcc ccccgtgttc cggctgcgcg cctcggcttc cacctgaaag
     4861 cctcgctgac cgaccgtatc gatgccaatt tggactacta ccgcgtgttc gcccaaaaca
     4921 aactcgcccg ctacgaaacg cgcacgcccg gacaccatat gctcaacctc ggcgcaaact
     4981 accgccgcaa tacgcgctat ggcgagtgga attggtacgt caaagccgac aacctgctca
     5041 accaatccgt ttacgcccac agcagctttc tctctgatac gccgcaaatg ggccgcagct
     5101 ttaccggcgg cgtgaacgtg aagttttaaa atcggacagg caaacaaaaa aggttttcag
     5161 acggcattct gaaaaccttt tttcttttta atcaatttgt taggaaatat ggcaaagaca
     5221 aagaacaaac cactatgcca tcattcccat tttttttgtt tcatcattct tactttcacg
     5281 ccgtcattct cgcaaaagca gaaaaatcaa aagcagaaac ccaagatccc gtcattcccg
     5341 cgcaggcggg aatccaattc gttcggtttc agccgtttcc gataaatcac tgtaacgtta
     5401 agttcccgga ttcccacttt cgtgggaatg acggcgtaaa ggtttctgtt ttttaagttt
     5461 taggtaacta ataagtcgtc attcccgcgc aggcgggaat ccagtctctt cgttttcagc
     5521 cgtttccgat aaatcactgt aacgttaagt tcccggattc ccactttcgt gggaatgacg
     5581 cgtaaaggtt tctgtttttt aagttttaag taactaatga gttgtcattc ccacgcaggc
     5641 gggaatccgg tctcttcggt ttcagtcgtt tccgataaat cactgtaacg ttaagttccc
     5701 ggattcccac tttcgtggga atgacgcgga aaggtttctg ttttttaagt gttaggtaac
     5761 taatgagttg tcattcccgc gcaggcggga atctaattcg ttcagtttct gttatagtgg
     5821 attaaattta aatcaggaca aggcgacgaa gccgcagaca gtacaaatag tacggcaagg
     5881 cgaggcaacg ccgtactggt ttaaatttaa tccactatat ttctgataaa tcaccgtaac
     5941 gttaaatttc cagattccca ttttcgtgaa cataacggtg tgaacattag caaaaattca
     6001 aaaaaccaaa cttcctcatt cgtgtgaatg atggtactga tattttctat ttcagttcgc
     6061 aaaattaatc tttcaacaca tcttgcacag ccgcctgaat cttagccgcc gctgcctcga
     6121 tttgccgttg ttcggcagat gggtttgccg ctgtttcaac cggagcagtt tgtaaagcag
     6181 gctcagactc aggctcggat tcgatttgcg gcgcagtcag cggcaaacgc gccaatacag
     6241 tgctaacgcc agttacttta tcgccaatcg ccacttgcgc ctgcgcatcg acaggcagat
     6301 acatatccac gcgcgaaccg aagcggataa agccataacg ttcgccgcgg gacagtttcg
     6361 cacctgcttg ggtgtagcac aaaatacggc gcgccaccaa accggccact tgaacaaaag
     6421 taatttcacg accggaagcc gtagtcgcca acaccgcatt acgttcgttt tccgtgctgg
     6481 ctttgtccaa atccgcattc acgaatttgc ctttgttata gaccactttc gttaccgtac
     6541 aatcggcagg cgatttttgc gaatgcacgt tgaacacgtt cataaaaata ctgattttca
     6601 aagcatcgac atcacgatac ggatcgcgtg cgcgctcgac caccacgata cggccgtcaa
     6661 ccggactcaa cactgcttca ggattttgcg gaatctcacg cgcagggtcg cggaaaaact
     6721 gcagggcaaa tacggtaaac acccaaaacg gcaaagacca ccagccgcaa cacatcgaca
     6781 ccagcaggct caaagccaaa ccgccgccaa taatcggcca gccctcacgg gcgataatcg
     6841 ggtgggggta aagacggttc atttgtcatc cttttgttca agcgggttaa aattgccgcc
     6901 attataacgg aatccgccgc gttccgccga caaatgccgt ctgaacgcgg cttcagacgg
     6961 catccgagaa agtgctacaa taccagtttt cctttatttt tcaggcggca tccattatgt
     7021 acaagcatca tccccaacat tcgcacgaca acgacaccat ccgcatccgc ggcgcgcgca
     7081 cgcataattt gaaaaacgtc gatttggaca ttccgcgcca caagctcgta gtggtaacag
     7141 gcttatcggg cagcggcaaa tcgtcgctgg catttgatac tttatatgcc gaaggtcaac
     7201 gccgttatgt cgagagcctt tccgcctatg cgcggcagtt tttgcagatg atggacaaac
     7261 ccgatgtcga tttgattgaa ggtctgtcgc ctgcgatttc tatcgagcaa aaatccacca
     7321 gccacaatcc gcgttccacc gtcggcacgg ttacggaaat ccacgactac ctgcgccttt
     7381 tgtacgcccg cgtcggcacg ccgtattgcc ccgaacacaa tctgccgttg tcgagccaaa
     7441 ccgtatcgca gatggtcgat gccgtgttga agctgccgga agacacgcgc gtgatgattc
     7501 tggcgccggc ggtgcgcgag cgtaagggcg agtttgtcga tttctttgcc gacttgcagg
     7561 cgcagggttt tgcgcgcgtg tgcgtggacg gcgaggtcta tcagcttgac gaagtgccga
     7621 agctggaaaa aaacatcaag cacaacatcg acgtggtcat cgaccgcgtg aaagtgaagg
     7681 cggacatcaa gcagcggctg gcggaaagtt ttgaaaccgc gctgcgccac ggcaacgagc
     7741 gcgcgctggc gatggagatg gacagcggcg aagaacattg gttttccgcg cgtttcgcct
     7801 gccccgtatg ttcgtacagc ctgcccgaat tggagccgcg cctcttttcg ttcaacaacc
     7861 cgatgggctc ctgcccgact tgcgacggct tgggtaacac caatttcttc gactccgaaa
     7921 aagtggtcgc gcatcccgaa ttgtcgctgg cttcgggcgc gattgacggc tgggacaagc
     7981 gcaaccagtt ctatttccaa atgattcagt cgctggcacg gcattacggt ttcgacgtgc
     8041 aggctgcttg ggaaacccta cctgcaaaaa tcaaaaaagt cgtgttgcac ggctcgggca
     8101 aagaagtcat tgatttcact tacctgtccg aacgcggcac caccttcaac cgcagccacg
     8161 ccttcgaagg catcatcccc aatctcgaac gccgctaccg cgaaaccgac agcgaaaccg
     8221 tgcgcgaaaa actgcgcgaa taccaaaacc accgcgcttg cccgagctgc ggcggcgcac
     8281 gtttgcgcaa agaagcccgc tacgtttacg tcggcggcga gccgttgcac gaagtttccg
     8341 cctggccgct gaccaaaacc caccgattct ttgaaacgct ggatttggac ggcaacaaaa
     8401 aacaaatcgc cgaaaaaatc ctcaaagaaa tcaccgaacg tctcggcttt ttgattaatg
     8461 tcgggctgga ttacctgaat ctctcccgtt ccgccgaaac cctttccggc ggcgaagccc
     8521 agcgcatccg cctcgccagc caaatcggca gcggcctgac cggcgtgatg tacgttttgg
     8581 acgaaccctc catcggcctg caccagcgcg acaacgaccg cctgcttgcc accctcaaac
     8641 gcctgcgcga tttgggcaac agcgtcatcg tggtcgaaca cgacgaagac gccatccgcg
     8701 aagccgattt cgtcgtcgat atgggcccgg gcgcaggcga acacggcggc aacgtactga
     8761 ttgccgacac gcccgaaaac gtcgccaaat gcgagcaatc cattaccgga caatacctca
     8821 gcagtaaaaa atccattgcc gtgccgtctg aacgcacgcc cgtcaatccc gaccggatgc
     8881 tcgtcctcaa aggtgcgcgc ggcaacaacc tcaaaaacgt taccctcgaa ctgccgctcg
     8941 gtttgattac ctgcatcacc ggcgtatccg gcagcggcaa atccaccctg attaacgaca
     9001 ccctcgccaa aatcaccgcc cgcgaactca accgcgccca agaagaaccc gccccatacg
     9061 acgacatccg cggtctcgaa catctcgaca aagtcatcaa cgtcgaccaa tcccccatcg
     9121 gccgcacccc gcgctccaac cccgccacct acaccggcct gttcaccccc atccgcgaac
     9181 tcttcgccgg catccccctc tcgcgcgaac gcggctacaa cgtcggccgc ttttccttca
     9241 acgtcaaagg cggccgctgc gaagcctgcc aaggcgacgg cgtgattaaa gtcgaaatgc
     9301 acttcctgcc cgacgtgtac gtcccctgcg aagtctgcca cggcaaacgc tacaaccgcg
     9361 aaacgctcga aatccaatac aaaggcaaaa acatcagcca agtcctcgac atgaccgtcg
     9421 aagaagcccg cgaatttttc gacgccgtcc ccaccgtatc gcgcaaactg caaaccctga
     9481 tggacgtagg tctcggctac atccgcctcg gccaatccgc caccaccctc tccggcggcg
     9541 aagcccagcg cgtcaaactc gccttggaac tctccaaacg cgacaccggc agaacactct
     9601 acatcctcga cgaacccacc accggcctac acttcgccga catcgccctg ctgctggaag
     9661 tcataggtcg tctgaaaggc aaaggcaact cgattgtgat tatcgagcat aatctagacg
     9721 tgattaaaac cgccgattgg attgtggact tagggccgga aggcggcgat ggcgggggca
     9781 ggattattgc ttgtggtagc cctgagcagg tggcgaaagt taaggggagt tatactggga
     9841 agtatttgaa ggtggttttg aggtaagttt aattcaaaat taaataggag attattaaga
     9901 tgaaaccgtt aaaaacatta gaatttggat ttgtggatgc tgcaaactac agaagaagag
     9961 aaaataaaga tttatttaac cgaatatttg taaaaggaga atatttggat gaattatgtg
    10021 aaccaaatat ttcgttttta atcggagaaa agggaactgg aaagacagca tatgctgttt
    10081 atttaactaa taacttctat aaaaacatac atgccactac taagtttgtt cgtgaaaccg
    10141 attattcaaa atttattcag ctaaagaaag caagacactt aactgtttca gattttacaa
    10201 gtatttggaa agtcatttta tatctgttga tatcaaatca aatcaaatgt aaagaaaacg
    10261 gaatattatc ttcaatattt aataaattta aagccttaga tgaggctata aatgaatatt
    10321 attatggcgc ttttgatccg gaaattgtac aagcaataac tttaatagaa aattcaaaag
    10381 aagctgcgga aatgattttt ggaaaatttg ttaaactagg tgaagaggaa tcccaacaaa
    10441 taacttttac agaaagtaaa ttccaagcaa atttaggttt tattgaaaga aaatttaaag
    10501 atgctttatc tcagttaaag ctaaaagata atcatatttt gtttattgat gggatagata
    10561 ttagaccatc acagattcca tttgatgaat atcatgagtg tgtaaaaggt cttgctaacg
    10621 ccatatggat gttaaataat gatatcttcc cttccattaa agatagtaag ggaaggatga
    10681 gagttgtgtt attgattaga cctgatatct ttgattcatt aggtttacaa aatcaaaata
    10741 ccaaacttca agataattca gtatttttag actggaggac ggattataaa tcttatagaa
    10801 gttcaaagat ttttggcgtt tttgatcatc ttttgagaac ccagcaagaa aaacaagata
    10861 gtttagaaaa aggcaactca tgggattatt attttccatg gaatgctcct aatttacatg
    10921 atgagtataa aaatttaact tcatttatta gcttcctaag aaaatcgtat tatcgacctc
    10981 gcgatattct tcagatgctt actttgctac aaaaaaataa gaaaagtaag gaagattatg
    11041 tcgtagcaga agattttgat aatacttctt ttcaaagaga atactcgata tatttacttg
    11101 gtgaaatcaa agatcatctt ttgttttatt atagtcaaag tgattatcaa aatttcctga
    11161 aattttttga atttttaaac gggaaagata gatttaaata tagtgatttt ttaaaagcat
    11221 ttgaacgttt gaaaaagcac ttacaaacaa catcagtgga aatacctaaa tttatgagta
    11281 ctgctaatga gtttttgcaa tttttatttg acttgaatgt tattgcttat ttagataacc
    11341 cagaagatga aacgaaacca tatatccatt ggtgctttaa agatagaaat tatgcaaata
    11401 tttctcctaa aataaaaact gaaactgaat atttaatatt ttcaggatta tcaaaagccc
    11461 ttgatgttgg tactccattt aagaacaaac agtaacttgt atcccaacca accgtgtgcg
    11521 ctacgcacac ggttggtagc tgacgtgttg cagtttcaac agagaaagcc gtagcctgca
    11581 tacccaaccg accgcgtgcg tggctgtgcc acacgcccta cctgtgaatt acacggaact
    11641 tcaaagccaa tacgagagct ttgcaaaatt cctttccctc ccgacagccg aaacccaaac
    11701 acaggttttc ggctgttttc gtttcaaata tccactaatt ttaccccaat acccccttaa
    11761 tcctcccagg atacccgata atcaggcatc cgggccgcct tttaggcgca aacaggcaca
    11821 cttagcccga gacctttgca aaattcccca aaatccccta aatgtcttgg tgggaattta
    11881 ggggattttg gggaattttg caaaggtctc tgactgcccc aaaagggaaa aaataatgtg
    11941 ggtatgttga ggctgcctct ttttgaaatg acaaacaaaa tgccgtctga aacccgattt
    12001 ccggtttcag acggcatttg atgtttaatc agttgtcaaa cctgattatg ccaaaccttt
    12061 tgccttcaac acttccagca tcgtcgtgcc caactcggca gggctgcgag tataggcgat
    12121 accggctttt tcgaaagcgg cgaatttttc ttcggcagta cctttaccgc cggaaataat
    12181 cgcaccggcg tggcccatac gtttgccttt aggagcggta acaccagcaa tgtaacctac
    12241 aacaggtttg gttacgttgg attggatgta ttcggctgct tcttcttccg cagtaccgcc
    12301 gatttcacca atcatgatga tggcgtcggt atctgggtct tcttggaaga gtttcagtgc
    12361 gtcgatttgg ttcataccag gaatcgggtc gccgccgata ccgatacagg ttgattgacc
    12421 caagcccagt ttggtggttt gtgccacggc ttcgtaagtc aatgtaccgg aacgggaaat
    12481 aataccgatg cggcccggag tgtggatgtg gcccggcata atgccgattt tgcactcgcc
    12541 cggagtaatc acgcccgggc agttagggcc gaccaagcgg gtaccgttgc cgttggtttc
    12601 caagtaacgt ttggctttga gcatgtccaa agtaggtacg ccttcggtaa tcacaacgac
    12661 caagcctacg cctgaatcaa cggcttcaac gatagaatcc aacacaaacg gagcgggaac
    12721 gtaaattacg gatgcatccg cgccggtttc tttaacagct tctttcatgg tgttgaatac
    12781 aggcaggttc aggtgggttt gaccgccttt gcccggggta acgccgccga caactttagt
    12841 gccgtaagcc agagcttgtt cggagtggaa agtaccgttt ttaccggtga aaccttgaac
    12901 caatactttg gtgtctttat taatcaatac gctcattctt ttctccttag gcgtttacgg
    12961 ctgcaacaat tttttcggct gcgtcattca ggccgtctgc agaagtcagt ttcaggcctg
    13021 attcgttcag gattttcgcg ccgagttcgg cgttgttacc ttccaaacga acaacgacag
    13081 gaacgtcgac gttgatttct ttaacggctg ccacgatggc ttccgcaatc atgtcgcaac
    13141 gtacgatacc gccgaagatg ttgatcaata cgcctttaac ggatttgtct tccaaaatca
    13201 gtttgaacgc ttcaaccacg cggtctttgg ttgcgccgcc gccaacgtcc aagaagttgg
    13261 caggttgacc gcctttgagt ttgatgatgt ccatggtggc catcgccaaa ccggcaccgt
    13321 taaccataca gccgatgttg ccttccaggg caacatagtt caggtcgaat tcagaagctt
    13381 tcagttcacg ttcgttttct tgagatttgt cgcgcaattc agcgattttt ggcaggcggt
    13441 agagcgcgtt gctgtcgatg ccgattttgc cgtccacgca agccagtgcg ccgttttcgc
    13501 gaactgccag cgggttgact tcaaacaggg cgaagtcgtt ttcgacaaac gctttgtacg
    13561 cgccggtcat cagtttgacg aactcgttga tttgtttgtc tttcaagccc agttggaagg
    13621 caacttcgcg agcttggcaa ggttgcaggc cgaccagcgg atcaacggtt actttgaaga
    13681 ttttttctgg agtttcggca gcaacttttt cgatttctac gccaccttcg gtagaagcca
    13741 tgaatgtaac gcggcgggta gaacggtcaa ccactgcgcc caagtacagc tcggtttgaa
    13801 ccggatacat gtcttcgcaa accaaaacgc tgttgacagg ttggccgttg gcatcagttt
    13861 ggtaagttac caagttggtg ccaatcaggc tttcagccac ttctttagct tcttcgcggc
    13921 ttttaacgac ttttacgccg cccgctttac cgcggccgcc ggcgtgtact tgtgctttga
    13981 caacagcgaa tttgccgccc aatttgtcgt aagctgcagc ggcttcttcg ccgttgtgtg
    14041 ccaaaatacc gccttgtacg ggcaaaccgt agctagccag cagttcttta gcctgatact
    14101 cgtgtaaatt catggatttc tcctttagat tagtggaatg ccccgagttt gcctccaaat
    14161 ggcaagcccg tgcgtttcag actacctttc ggcggtcgga aaatcgtcgt ttcacttgct
    14221 gaaaggcaac cacatccgcg ccagcagata catggctgca ccgcctcccc cgagaataaa
    14281 aaagacgatg ccttttttac gcgaattcgg acagagcaga taaacggcaa ggctgaaact
    14341 gataaacaac aatgccacgc gccaaaccaa ttctttatct ttgaaacggt tgagcgtata
    14401 agtttcggtc atcatcacat acatctgcca caatgcggcc atacacatgc tgatgatgcc
    14461 cgccggtata aagaaaatac cgactatttc tttgttcata atgaatgtaa atgctgtttg
    14521 tttgtctgac ggaattataa atcagaaaac gaagctctcg gaacattcaa tttgtcggag
    14581 gccttaatgt ctatcaaccg tgcaaagcgc gtttgtcggc cgccaatgca gcttcgtgaa
    14641 ccacttcgga caaggttggg tgggcatgga taatgcgggc gatgtcttcg ctgctggcga
    14701 agaattcgag cgcagtcacg ccttcggtaa ccaattcgct gacaaccgga ccaatcatgt
    14761 gtacgcccaa gatgcggtcg gttttggcgt ctgccaacac tttaaccgta cctttggctt
    14821 tgcccattgc caatgcgcga ccattcgcac caaaacctga agtacctttt ttgtactcca
    14881 cgccttcggc tttaagctgt tcttcggttt tacccaccca agcgatttca ggatcggtgt
    14941 aaataacaaa cggtacgttg ttgaaatcga tatgcggttt ttgaccggca atgcgctcgg
    15001 caacggcaac gccttcgtcg ctggctttgt gtgccaacat cgggccgcga accacgtcgc
    15061 cgattgccca tacgttaggc aggttggtac ggcattcgcc atctactttg ataaagccgc
    15121 gctcgtcttt ttccaagcct acggcttccg cgttcaggcc tttggtgttt ggaatacggc
    15181 cgatggcaac gatcagtttg tcgaatactt cggttttggc ttcgccggca gcagtttcgt
    15241 aagcaacgga aacacctttg ccttcagact tgatgtcgcc gattttcacg cccaattcga
    15301 tgctcagacc ttgctctttg gtgaagtatt tgaaggcttc tttggcgatt tgttggtcgg
    15361 cggcagccag gaaggtcggc gcggcttcaa gaatggtaac ttccgcaccc acgcggttcc
    15421 atacggaacc catttccaaa ccaatcacac cggaaccgat cacgccgagt ttggcaggta
    15481 cttcggtcag gttcaatgcg ccttcgttgt ccaatacgtt cacattgtcg atagcgactt
    15541 gtggcagcgg acgcggtacg gaaccggtcg ctacgatgac gtgtttggct tcgataacgg
    15601 ttttctcacc tttgttatcg acttcgattt ggtaagcatc gccatttttg ccggcaaagg
    15661 aagccgtacc gaacaggctg gttactttgt ttttctggaa caggaatttc acgccgccgg
    15721 tcagtttggt tacgatggca tctttgcgct caatcatttt ggccacgtcg aattttacgt
    15781 cgccgacagt gataccgtgt tcggcaaact cgtgttgcgc tgcgtggaaa tgttcgctgg
    15841 attgcaacag ggctttagaa gggatacaac ctacgttcaa gcaagtaccg cccaatgcag
    15901 gggcattgcc tgctttgtta acgcctgcat cgacacaggc agttttgaaa cccagttgtg
    15961 cggcacggat ggcggcaacg tatccgcccg ggcctgcacc aatcactact acatcatatt
    16021 gagacatcgt gtcatccttt gcttttagca gtatctcaaa ataaaaacga tacttttcgt
    16081 ttctttttta ttttgaatga ctgcgttttg atggttttaa gaaaaagccg tctgaaagtc
    16141 catatctgcc gattcattcg aatcaaatgt ttcagacggt ctcaatcgtt tgttgagcct
    16201 attgtagcac gatactacat tacgctacat ctaaatatag tggattaaat ttaaatcagg
    16261 acaaggcgac gaagccgcag acagtacaaa tagtacggca aggcgaggca acgccgtact
    16321 ggtttaaatt taatccacta taaaaattca gacggccttt tcattaacaa ataaaaggcc
    16381 gtctgaaacg attacagatc caacaacagg cgggctgggt cttccaacgc gtctttaatg
    16441 gctaccaagg tcaatacagc ttcgcggccg tcaatgatac ggtggtcgta agacagagcc
    16501 agatacatca ttggacggac aacaacttgg ccgttttcaa ccacagcgcg ctctttagtg
    16561 gcgtgcatac ccaaaatcgc agattgaggc ggattgatga tcggggtgga catcatagaa
    16621 ccgaaagtac cgccgttggt aatactgaat gtaccgccgg tcagatcttc gatagcgatt
    16681 ttgccgtctt tggctttttt cgcgtaatca acaattgctt gttcgatgtc ggcaatgctc
    16741 atttggtcgg catcgcgcag gattggcaca accaaaccgc gtgggctgcc aatcgcgata
    16801 ccgatgtcga agtagccgtg gtacacaatg tctttgccgt caacagaagc attcacaacc
    16861 gggtattttt tcagggctgc aacagcggct ttaacgaaga aggacataaa gcccagtttc
    16921 acgccgtgtt ctttctcgaa tttttctttg tacttcgcac gcaagtccat gattggtttc
    16981 atgttgactt cgttgaatgt agtcagaatg gcgttttctt gttgagaagc caagaggcgt
    17041 tctgcaacac gggcacgcag gcggctcatc ggtacgcgtt cttcaggacg tgcgccggca
    17101 ggaagtgcaa cggcaggagc agcggctgcg gcaggtttgg ctgcggcatt ttgtacgtct
    17161 tctttcaata cgcgaccgtc acggccggaa ccttgcaatg cgttcacgtc aacaccgctc
    17221 tctgcagcca gtttggcggc ggcaggcata gcggcgttgt tttgtgcagc agcaggagcg
    17281 gcggccgggg cagcttcggc aggagcggcg gcaggtgctt cagcagcggc agtagcagcg
    17341 gtatcgatac gtgccaaaac ttggtcggca acaacggttt caccgtcttg cgctacgatt
    17401 tcaaccaata cgccggcttg tggagaaggt acttccaaaa ccactttgtc cgtttcgata
    17461 tcgatcagga tttcgtcacg ggcaactgct tcgccaactt ttttcttcca ttccaagagc
    17521 gtgccttcag atacgctttc agacagcata ggtactttta catcaataat cattttgtgt
    17581 ctccaatggc cctttcagac ggcctgttgt gtgtttttca ttctgcatat gggtgttccg
    17641 cctgagcagc ctcagacgga atgctgcttg gtttacaacg ccaaagcgtc ttcaaccaat
    17701 tgtttcaatt gagcaatgtg tttgcttgag tagcccactg caggcgatgc gctgcttgga
    17761 cgaccggcat aagacagttt ttgctcttcg ctgataacgt cttcgatgcg gtggcggatt
    17821 tggtagaacg cgccttggtt tttcggctct tcttgcgccc aaaccacaga ttttgcgttc
    17881 ggatatttcg ccagttcggc tttaacctca tcgtatggga acggatacag ctgctcaacg
    17941 cggacaatcg caacatcatc ttccagttta cgttcggctc gaccggcttc caagtcatag
    18001 taaacctgac cggcacacaa taccacgcgt ttcacgctgt cgttgcttgc gcgttcggcg
    18061 gtatcgccga caaccggacg gaaagtcgaa ccttcggtga agttttccag cgggctcatt
    18121 gcccctttga atcgcaacag gcgtttggac atgaaaatta ccagcggttt gcggtatgaa
    18181 cctaagactt gacgttgcaa gaggtggaac atttgcgacg cttcagatgg catgatgact
    18241 tgcatattgt tctcagaaca cagttgcaac cagcgttcta cgcgtgcaga agagtgctca
    18301 gggccttgac cgtcgtaacc gtgcggcagg atggtggtca gaccgcacaa acggccccat
    18361 ttggtttcgc ctgaagacag gaattggtca atcgtcactt gcgcgccgtt ggcgaagtca
    18421 ccgaattgag cttcccaaat ggtcagtttg tcaggtgcgg agcaggcaaa gccgtactcg
    18481 aacgccatta ctgcttcttc gttcaaaatg gagtcgataa ccaagaactc gcccatacct
    18541 tcgcccatat ggcgcagagg aacataagta ccgtcatccc atttttcacg tttttgatcg
    18601 tgcaatacgg cgtggcggtg cgagaacgta ccgcggccgg agtcctcacc ggagatacgc
    18661 acgccatgac ctttggttac gaggctggca tatgcgaggg tttccgccat accccagtcg
    18721 ataggctgtt tgccggatgc catggcttta cgcgcttcaa tcacacgttt tgcagtcgga
    18781 tgcagggcaa agccttccgg tacggcggta aacttctcgg tgagacgctc aatatctgcg
    18841 gcaggtaaac cggtttcgat gtgttcgcgc caatctttac cttgatattt gctccaatcg
    18901 atttgtgtgc gttggaaatt gctcaaagtt gtttgctcga cgtgttcgcc tttgtccaaa
    18961 gcatcacggt aggcttggat gtaagcatca gcttctgctt gagttaccac gccttcggca
    19021 atcagttgct cggtgtacaa agcacgcgca ccggggtgtt gcgatacttt tttgtacatc
    19081 atcggttggg tcaaggtcgg atcatcgccc tcgttgtgac cccatttacg gtagcagaca
    19141 acgtcaatca cgatgtcttt atggaatttt ttgcggtaat ccaaagcggc ttggatggca
    19201 aagcaaacgc gttcgggatc atcgccgttg acgtggataa ccggggcgga aaccattttc
    19261 gcgatatcgg tacagtgtac ggttgaacgg gtatcgcgga tatcggaagt ggtaaagccg
    19321 atttggttgt tgatgacgat gtgaaccgta ccgccggtgg tataaccgcg cgttttagac
    19381 aggttgaatg tcgcttggtt gacacccaaa ccgataaatg cggagtcgcc gtgaatcaat
    19441 acgggcaaga ctttatcgcg gccgttttcg cccaaacgtt tttgcttggc gcgtacagaa
    19501 ccttccacta ccggattgac gatttccaag tgtgacgggt tgaatgccaa agaaacgtgc
    19561 atcggaccgt gcggcgtggc aatatcggag ctgaagccca tatggtattt cacgtcgccg
    19621 ctgggcagtt tgatttcggc acggccttca aactcagcaa acaaatcgcc gggttttttg
    19681 cccaaaatgt tcaccaaaac attcagacgg ccacggtgcg ccataccgat gatgacttct
    19741 tccacgccgt ctttaccggc gttttgaatc aggtagttca aaccggcaat cgcgctttcg
    19801 ccgccttcta caccaaagcg tttttggccg acatatttgg tatgcaggta gcgttccaaa
    19861 gtttcggcag cagtcatctc tttcaagata cggcgttttt gatcggcatt gtaatgcggt
    19921 gtggacaata cgctttcaaa ataattgcgt acccagcggc gctcttcggt attgggaata
    19981 tagatatatt ccaatgcgat gtggccgcag taggtttgtt tgaggttgct gatgatttgg
    20041 gacaaaggca gtttgccgcg attggcaaaa tcgccctcgc ccatattgaa ttgaagcgtc
    20101 atatcggcat ctgacagacc gtggaatttc ggatcgaggg cttcaatatc gcgcgggggg
    20161 atacgtttga gcggatcaag ttgggctgca cccacgcctt ggatacgata ggcggaaatc
    20221 agccgtaaaa cgctgacttg ctttttcagc attgcctcat ccgcaccgcc cgcaacggca
    20281 gatgcaattt tctttttcgc caaagtaaca aatgattcgc gaatcggtgt gtgtgcgaca
    20341 tcgacagcaa ccgtccccgg ctgtttgctc aaatcggtga aatactgctt ccatttttca
    20401 tcaaccgcat cggggttttc caaaaaagcc tcgtacaatt cctcaatgta aggtgcgttt
    20461 gaaccgaaca ggtaagagaa attgagtttt tcgtccatca tggcgtgcgc tcttttcttt
    20521 aaaatataaa caaagggtag aagtccgcgt cctgacggct aaactgatgc cgcctgaaaa
    20581 gacattttat tctaccctat tttaaaccgg attgcttgtc ttgatttcat ccgactacaa
    20641 tcagacggat tcgggaaaca atccggttct ctgatattgc ctgtttgaca atatattaat
    20701 gtatgttacc tctcgcctgg cggcacataa tcgcggcgtt ccgaaccagt ataaagctgg
    20761 cgcgggcggc cgattttcag cgaaggatcg ctaatcatct cgtgccagtg cgaaatccag
    20821 cccacgctgc gcgacagggc gaagatgacg gtaaacattt cggtcgggat gcccagcgcg
    20881 gacaggacga tgccggaata gaaatcgacg tttggataca gtttgcgttc gataaagaac
    20941 gggtctttca gcgcaatctg ttccaattcc atcgccagtt tgaatttcgg actgtcttcc
    21001 aagcccaatt ccttcaaaac ttcatagcag gtttcgcgca taatgctggc acgcggatcc
    21061 atattgcggt acacgcggtg accgaagccc atcagacggt atttgcgttg tttcacacct
    21121 tccatgtatg cggcaacatt agacacatcg ccgatttcgt ccaacatttt caacacggct
    21181 tcgttcgcac cgccgtgcga agcaccccac aggcaggcga taccggcagc aatacaggca
    21241 aacgggttcg cacccgaaga ccctgccaga cggacggttg aagttgaggc gttttgctcg
    21301 tggtcggcat gcaaaataaa gatgcggtcg agcgcgcgtg ccaaaacggg attgggtttg
    21361 tagtcttcac acggcgtggc gaacatcata tgaaggaagt tttcggaata agaaagatta
    21421 ttcttcggat aattgaacgg cagaccgttt gaatagcggt agcacattgc cgcaatggtc
    21481 gggattttag aaatcaggcg gtaaatcgcg attttgcggt gttcgggatt gctaatgtcc
    21541 aagctgtctt ggtagaacgc agacagtgcg ccgaccacgc cgaccatcat cgccatcgga
    21601 tgcgcgtcgc ggcggaaccc ccggaagaac caagtcagct gttcatgcac catcgtgtgg
    21661 cggcggacgg tattgtcaaa ttctgccttt tgctcgggag tcggcagttc gccgtaaatc
    21721 aacaggtagc agacttccaa ataatcggac ttttcggcca gctgctcgat ggggtatccg
    21781 cgataataaa gcaagccttg atcgccgtcg atgtaagtaa ttttagactc acagcttgcg
    21841 gttgaaacaa atccggggtc gaaggaaaac aaacctgtat tttttgtcag cccccgaatg
    21901 tcaaccacat cgtgcccgat gctggcttcc aataccggca gctccaaacc tgcctgaccc
    21961 ggtacgttga gtttgattga tttggacata ttattgctcc tttgcaagtt ataacccacg
    22021 ttactgcatc cgcagcacgt tctttgcctt cagacggctt ttgcattttg aaatctgcct
    22081 gcaggcagat ttcagatgcc tcatgcccgt ctgatttttt caagcatcgg gataaggtgc
    22141 cctttgtccg tttccgaatg cccgttaatc aaggcaagca attcttgatc ttgaaattca
    22201 aggatttcgg aaaactcgga cagctcttta tcgctcaaat gctcgaattc tttttccata
    22261 aacctgccga agattaaatc taattccaac aatccccggc gggtttgaaa acggattttc
    22321 cgtttggcaa tatcgtcaaa aaccatcatt tcttaaacgg cccgtttcaa cataatctct
    22381 ttaatcttac cgatggctcg ggtcggattc aagtgtttag gacatacgtc tacgcagttc
    22441 ataatggtgt ggcaacggaa caaacggtat gggtcgttca gattatccaa acgctcatta
    22501 gtgatggtat cacggctgtc cgcaatgaaa cggtaagcat tcagcaaacc ggacggacca
    22561 acgaatttat cagggttcca ccaaaatgac gggcaggcag tcgaacagca ggcgcacaaa
    22621 atacactcgt acaaaccgtc caactcttta cgctcttcct gagtttgcag acgctctttg
    22681 tccgcatcaa tcggattatc gttgacaaca taaggtttga cggaatggta ttgtttgaag
    22741 aactgggtca tatccacaat caggtcgcgg ataacaggca gacctggcag aggacggatt
    22801 ttaactggct gtttcaagcc acgcagatcg gtcaaacacg ccaagccgtt tttgccgttg
    22861 atgttcatac cgtccgatcc gcaaatgcct tcgcggcagg agcggcggaa agacaaggta
    22921 tcgtcttgtg ctttcaggcg taccaaagca tccaaaagtt tcacgtcggt cggttccaat
    22981 tccaactcgt aacgctgcat ataaggcttg gcatcaacat ccgggttgta acggtaaatt
    23041 tcaaaactca ttttttccat gagaagtgtt ccttttctca tcaactgccc aatgattttg
    23101 tattataaat tcaagcagtt caatgtgggt atggttagat ttcagacgag cttatgaggc
    23161 gaaaaccgtc tgaaaacgca tcaataaacg cgcttggccg gtttgatgta ttccacgctc
    23221 aaaggcttgg tatgtaccgg tttgtaagac aaagtattgg tatctgaatg gtacagcgta
    23281 tgtttcatcc agttttcatc atcgcgctca ggatggtcgt ctgaagcgtg cgcaccgcgt
    23341 gattctttac gtgcttcggc agacaccaaa gtcgctttcg ccacttcaat taggttatcc
    23401 aattccaaag cctcgatacg cgcggtattc cacactttgc tcttgtcttt gatttcggta
    23461 cgtttcacac gctcggcaat cgccatgact tctcgaacgc ctttgctcag aatctcatca
    23521 gtacggaaca cgccggcgtg caattgtacg gagcgttgca gttcgcggcg caatgcatca
    23581 acgttttcac catcggtttg gctgtccaaa cgctcgatac gttggcgggt caactcacca
    23641 gcattagcag gcaaaggttt ccagtcgctt tgctctttga tgaatttaat catgctgtcg
    23701 ccggcagctt taccgaatac caccaagtcc agcagggagt tcgtacccaa acggttcgca
    23761 ccgtgtacgg aagcacaggc gcactcacct gcggcataca ggccttttac aggcacttcg
    23821 tattcgtctc cttgcggcac aacgacttcg ccgtggtaat tggtcggaat gccgcccatc
    23881 atatagtggg tagtcggcac aacgggaatc gggtctttaa tcggatcgat accagcgaac
    23941 tgaatggaaa tctcgcggat gcccggcagt ttttccataa ttttttctgc gccgatatgg
    24001 tcgattttca gtaagacatg gtctttgttt ttaccgcaac cgcgaccttc gtagatttcc
    24061 atcgccatcg cgcgggaaac aacgtcgcga gaagccaagt cttttacggt cggcgcatag
    24121 cgttccataa agcgttcgcc gtcggcattc aacagaatac cgccctcgcc gcgtacgcct
    24181 tcggtaatca acacgcccgc acctgccacg ccggtcgggt ggaattgcca gaattccatg
    24241 tcttccaacg ggatacctgc acgcgcacaa atacccaaac catcgccggt attcatatag
    24301 gcattggtag aagacgcata aatacggccg ccgccgccgg tagcaaacat cacagcttta
    24361 gcgtggaaaa tataaacttc gccggtttcc atttccatgg cggttacgcc gacgacatcg
    24421 ccgttttcat cacgaatcaa atcttgtgcc gtccattcca caaagaattg cgtattggca
    24481 cggacatttt gttggtacaa agtatgcagc atcgcatgac ctgtacggtc ggcaaccgca
    24541 caggcgcgtt ctaccgcgcg tttaccgtgt tcggcagtat ggccgccgaa aggacgctga
    24601 taaattttac cgctttccac acggtcaaaa ggcataccca tgtgttccaa ctcaattacg
    24661 gcttcaggcg cggcgcggca cataaactca atcgcatctt ggtcgcccaa ccagtcggaa
    24721 cctttcacgg tatcgtacat gtgccagtcc caacggtctt cctgcacatt acccagagag
    24781 gcggaaatac cgccctgcgc cgctacggta tgcgaacggg tcgggaacac tttagacaaa
    24841 acggcacaat tcagaccgga tttggataat tggagggctg cgcgtaaacc tgcaccacca
    24901 ccgccgacaa tcacggcatc aaacttgcga acaggaaaac ccatacttac ccccaaatta
    24961 ctttaattga atacaccaag cagccgacca gccagacgat ggtggcaacc tgcaaaaaca
    25021 aacgcacgcc gaagggtttg atatagtcca tccacaaatc gcggataccc acccaagcgt
    25081 gcaagaatac ggcgatgaag ctcacttggg taaatacttt tacccaagtt tgactaaaaa
    25141 atgcctgcca tgccgaatat tctttaggca gggaaaatag aaccactaaa agtgcaacgg
    25201 tataaatcaa cataataacc gcagtcgcac gttgcatcgc ccaatcgcgc aaaccgtaat
    25261 gggcaccggt caatttacgt tctaccataa caacgctccc aaaacgacag tcaaaaccaa
    25321 tgcagaagca aatacggctt tagcggtatt gcgcgcagta ttcagctcaa ggcctttgtg
    25381 tgcatccaaa aataaaaagc ggataccggc gagagaatgg tgcagataag cccacaatac
    25441 accgattaaa accagcttga ccaaaggatg ggaaacaatg gcacggtaag tttcaaatgc
    25501 agactcttga ctcagggtac cggacaggaa atacagcagg aaaggcagca taataaacag
    25561 cccgaccccg ctgatgcggt gaaggatgga aactatcccg ggtatcggca gacggatgtt
    25621 cggcaaatcc agataaacag gacgcggttt ggcagacata gtcaattcct ctgtagttat
    25681 atatcgactc aaaaaccgta aacccattgc tacactgggt tacataaacg ttaatttacc
    25741 tgttttatcc cattttgaac agtgtatttt aaaaacaaac tgatagattt tgacaaacac
    25801 gcgtggaaac agcagattaa aagaaagatt tttagttttg ccgccccctt cccgacacgg
    25861 tatcagcatc cgaccccatc cccaaaaaca aaatgccgtc tgaaaaaatt tcagacggca
    25921 ttgttttgcc atatcggtca ggctttcaat ttcgcatcgg catcgcgcaa cgcggtacgc
    25981 agaccttcct cgataacggg atggtagaac ggcatatcca gcatttgcgg aacggtcatc
    26041 ttcatttgat gcgcccatgc caacaagtgc gccaaatgtt cggcggcagg gcctacgatt
    26101 tccgcgccga taaagcggcc ggtggctttt tcggcataca ggcgcatatg gcctttgttc
    26161 accagcatca cgcggctgcg gccttggttt ttgaacgata cttcgccgat gacaaattcg
    26221 tcggcttggt attgcgcggc aacctgcgcg tatttcagac cgacaaagcc gatttgcgga
    26281 ctggtaaaca ccacgccgat ggtgctgcgc cgcaaaccgc cgccgatatt cgggtaacgg
    26341 cctgcattgt cgccggcaat cttgccttgg tcggcagctt cgtgcagcag gggcagttgg
    26401 ttggacgcgt cgcccgcaat aaagatatgc ggaatgctgg tctgcatggt cagcgggtcg
    26461 gcaacgggta cgccgcgcgc atctttttcg atattgatat tttccaaacc gatattgtca
    26521 acgttcggac ggcggcccac ggctgccagc atatattcgg caacaaatac gcctttttcg
    26581 ccgtcctgct cccaatggac ttctacattg ccgtctgcat cgagcttgac ctcggtttta
    26641 gcatccaaat gcagtttcaa ttcttcgccg aacacggctt tcgcctcgtc tgaaacgacg
    26701 gggtcggaaa tgccgccgat gattccgccc aaaccgaaaa tttcaacttt cacgcccaaa
    26761 cggtgcaatg cctgacccag ttccaaaccg ataacacccg gcccgaacac ggcaacgcgc
    26821 ttaggcagcg tatcccatga gaaaacgtca tcgttgataa tcaaacgatt gcccaaagac
    26881 tgccattgcg gcaggatgac gggacgcgaa ccggtagcaa tcacgaaact ttttgccgta
    26941 atttgagtat gctcgtcgat ttggacggta tgctcgtcga taaatttagc cgaacccatg
    27001 atgcgcttgt cggcaggcca ctcttccaca tcggcaacga caaagccgac aaaacggtca
    27061 cgctcggatt tgacgcgctg catgacctct tcgccgttga cgacgatgct gtctttgtcc
    27121 aaatgcacgc cgaacgggtc ggtatgcaat gcgtgatgac gcgcctctgc ggcggcaatc
    27181 aagagtttgg aaggcataca gcccacgcgc gcgcaggtcg tgccgaacac attgttttca
    27241 atcaggtaaa cattatccga atgtaaacgg gcattgcgaa acgcacccat accggcagta
    27301 ccgccgccga ttacgacgac atccgcttga atttttttca taatctttgt ccttccgttt
    27361 tcgtctgaaa cattgccgac acagaacata tcggtttcag acggcattta aatattttcc
    27421 caatcaaaaa catcattcag ataaataaaa acaaaatttc gattgagtgc aacaatatcc
    27481 aaattctctt ataaatatag agttattcgg agcggcagtg tagaaccgcc cgaataactc
    27541 tatccggtat atcagacagt ttaatcaaac cgccttaagc aaacagctat cagtttttag
    27601 ccaagtaagc ttccaaatct tcgctgccgc cgatgtattt gccgccgatg aagacttgag
    27661 gggcagtcat cttgccggta atggcgcgaa cggaagtaac ggttgcatct ttgcccaata
    27721 cgatttcttc gtaagacaaa cctttgtctt gcaaagcttg tttggcttta gcgcagaatt
    27781 ggcaacctgg tttagtgaaa attgccacag actcttgagc cttccaatcg ggagcaacga
    27841 attgcagcat agtatctgca tcggatactt tgaacggatc gcccagttct tcaggttcga
    27901 tgaacatttt ttcaaccacg ccgtcgttaa ccagcatgga gtaacgccaa gagcgtttac
    27961 cgaagcccaa gtcttcttta ccgaccagca tacccatacc ttcggtaaat tcgccgttgc
    28021 cgtcaggaat catgtagatg ttgtcggatt cttcttcggc agcccaagcg ttcattacga
    28081 acgtatcgtt tacagatacg cagtagattg cgtcaacgcc gttttctttg aacgcgccga
    28141 acaattcgtt gtaacgcggc aggtgtgaag aagaacaagt cggggtaaat gcaccgggca
    28201 gggagaatac gactactttt ttgcctttga acaaatcatc ggtagacaca tctttccaag
    28261 tgtcgccgac gcgggtgcgg aatactacgg aaggtacttt ttgaccggta cgatcttgca
    28321 aagccatttt aagctcctta atagattggt ttgagaaata attgtgaatc gctgggcatt
    28381 ctaccccatg caaaatgatt tgtataattt atagttcaaa taatataaat tatttaaaac
    28441 tatcaaacac aactccgatt tgcctgtttc ccacatataa agccgaatta gaaaaaaaca
    28501 agctgcaatg aaaagatttt ccggcaactg cgcccttgcg ccgcaaatca ggacaaaata
    28561 ccgtctgaaa ggctttcaga cggtatttat tcatacggtc tcggcttatt tcgccaattc
    28621 ggcacgcagt tttcgggtta cgttcatcat cacttggagt tgttccagag tttctttcca
    28681 gccgcgtgtt ttcaggccgc agtctgggtt aacccacaga cgttcaaacg gtacaacctc
    28741 gatggctttg cgcaacaggt gctcaacttc ggcttcagtc ggtacgcgcg ggctgtggat
    28801 gtcgtaaacg cccgggccga tgtcgttcgg gtatttgaat tcgccgaatg cggtcaacaa
    28861 ttccatgtca gaacgtgaag tttcgatggt aatcacgtcc gcgtccatgc tggcgatggc
    28921 cggcaggatg tcgttgaatt ccgagtaaca catatgggtg tggatttggg tactgtcttc
    28981 gcaaccggtt gacgacaggc ggaaggcttc gcttgcccaa gccaagtagg catcccaatc
    29041 ggcacgtttc aacggcaggc cttcgcggat ggccggttcg tcgatttgaa tcactttaat
    29101 accggctttt tccaaatcca atacttcgtc atttaaggcc aaggcgattt gtttgcacac
    29161 ggtgctgcgc ggaatatcgt tgcgcacaaa tgaccattgc agaatcgtta ccggaccggt
    29221 caacatgcct ttcatcgggc gtttggtcag ggtttgggcg taggttgacc aagccacagt
    29281 catcgcgttc gggcggctaa catcgccgta aatcaccggt ggtttcacgc agcgtgagcc
    29341 atagctttgt acccagccgt attgggtaaa ggcaaagcct tccagcaatt cgccgaaata
    29401 ttccaccatg tcgttacgct cggcttcgcc gtgtaccaac acatccaaat tgagtttttc
    29461 ttgctcttca atcaccaagg cgatttcttt tttcatcgcg gcttcgtaat cagcgtcaga
    29521 caattcgcct tttttgaacg cggcacgcgc ttggcggatt tcagtggttt gcgggaacga
    29581 accgatattg gtggtcggca gcaacggcag gttcagccat gcttgttgcg ctttaatgcg
    29641 ctcggcaaac ggtgatttgc gttggtcggc atctgccggt aaatcagcca agcgtttggc
    29701 cacttcggcg cggtggattt cggtgctgtt ggcgcgtgaa tcagcagcgg cttggctgtc
    29761 ggccaattcg gcggcaacgg cttcgcggcc ttggttcaat gcagttttca acacgcgcaa
    29821 ttcttgcgtt ttctgcaagg tgaaagccaa ccatgagaac aaggcaggtt tgttgacttt
    29881 cagcttttct tctaccgaca aatcgtatgg cgtgtgcagc agcgagcatg agctggaaat
    29941 ccacaagcgg tcgcccaatt tggctttcaa cggctcaagc gtggctaata cgttgtttaa
    30001 attggcgcgc caaatattac ggccatcaat cacaccggca gacaacactt tgccgtaatc
    30061 agcaaatgcg tccaattgct cgggcgcgcg caccaaatcg atgtgcaaac cggccacagg
    30121 caaggctttc agacgacctg cgtgtgcggc aaccgaaccg aaataagtgc tcaacaagat
    30181 tttggcattc acttggctca aagtggcgta aacgtcttgg tacgcatcca accacgcttg
    30241 cggcaggtca accgccaatg ctggctcatc gatttgaatc cattcggcac cggcagcggc
    30301 caattcggtc aggatttcaa cataaaccgg caataatttt ggcaacaggc tcaagcggtc
    30361 aaattcgaca gcgccttttt ctttaccaac ccacaggaag gtcaacggac caacaacggt
    30421 cggtttggct ttcaggccca aagcttgggc ttcttgcagc tgttgaacgt agtgtttggc
    30481 gttcgctttg aattcggtat cggcgtggaa ttcaggcacc aagtagtggt agttggtgtc
    30541 gaaccatttg gtcatttcga tagcgaattg gtctttattg ccgcgtgcca gttggaagaa
    30601 ttgttccaaa gacaggtttt ggctgtcgaa gccgaaacgg gcagggatgg caccggtagc
    30661 gacttgcagg tcgaggatgt ggtcgtagaa agtgaaatcg cctacagcaa cgtaatcagc
    30721 gttggcagcg gcctggtgtt tccagttttt ctcgcgcaag tctttagcaa cagccagcaa
    30781 ttcttgctcg ctgatttctt tgcgccagta tttttcttgt gcgaatttca attcgcggaa
    30841 ggcgccgaca cgcgggaagc ctgagaaatg taatgttgtc atgttgaact ctccagttgg
    30901 attttgtaag gaccggttca atccgaatcg gccggtattg taaatcagaa tggctcggat
    30961 ggggcggtgt tttgcttttt agacacctta gatgccgtct gaaactgttt gatacggctt
    31021 taagggcgca cgcctaaaat atggcaaatg gcgtaagtca gctcgctgcg gttaagcgta
    31081 tagaagtgga aatctttcac gccttcgcgg gacaggactt tgaccatatc gatggcgata
    31141 cttgccgcca ccagattgcg cgtaccttgg tcgtcgtcca aaccttcata catttgcgac
    31201 agccagcttg ggattttgac gttggttact tgagccattt taccgagctg cttgaagttg
    31261 gtaacaggca aaataccggg gacgatttcc acatcgatgc ccaacatcac gcagcggtcg
    31321 cggaagcgca ggtagcgttc cacatcgaag aagaattggg tgatgacgtg gttcgcgccc
    31381 gcatcgattt tgcgtttcaa attaatcagg tcggcttgtg cggatttcgc ttcgggatgc
    31441 acttcgggat atgctgctac agaaatgtcg aagtcggcga cggagcgtaa tagcttaacc
    31501 aagtcttcgg cataaaacgg ttttttctcg taaccgggcg gctcgtcgcc gcgcagggcg
    31561 acaatgcggc ggatgccgct gtcccaataa tctttggcaa tttggcgcaa ttcgtcggga
    31621 gaagcgtcga tgccggtcag gtgcggcgcg gcttccaagc cggtttcttg cttaatgcgt
    31681 ttgacgatgc cgtgcgtgcg gtcgcgttcg ccggaatttg cgccgtaggt tacggatacg
    31741 aacttgggat gcagggtttg cagacggtgg atggaatccc acagcatcgt ttccatttgt
    31801 tcgtttttcg gcgggaaaaa ttcaaacgaa acgttgatgt cgcctttcaa atcggaaagg
    31861 ctgttgttta acgcattgat ttcttttgcg taattcatgg ctgcgtacct tttattatct
    31921 tttatatgat atttcaggca acataataaa attgcaccga atatgagtca atttcaaaat
    31981 tttcaggttt atcatgaaaa tttctaatat attacaaaaa tagttaaatt tccttgtaca
    32041 agccttctaa atttatgata aagtttgtta tgttataaca atccaactaa ggcgatatat
    32101 gaaaccgaat attcatcctg acaattatcg aaccgtcctt tttttcgaca gcagcgccaa
    32161 tgaaggctgg ctgattcgtt cttgcgccga aacacacgga aaaacgatgg tttggacaga
    32221 cggcaaagaa tatccactgt tttcgctgga tacttcttcc gcatcccatc ccgtctatac
    32281 cggcaagcaa cgcaacgtca acaccgaagg ccgtgccagc aaattcaacc aacgtttcca
    32341 atccgtcatg tcatcgttta gaaaggacaa ataatgcagg ttctgtcttc actcaaaacc
    32401 gccaaacaac gccaccgcga ctgtcagatt gtcagacgca aaggcaaagt ttacgtcatc
    32461 tgcaaaagca atccgcgttt taaagcgcgc caacgctgac cccctgccct tcctgcttcg
    32521 gttgtaaatc ccctcctacc gcaagccctt ttcatcaaat atgccgtctg aaacagaaaa
    32581 atgcgtttca ggcggcattt tttccgtcaa tacgaatccg gaatgtgttt gaaatacagc
    32641 ataaacagat accaaaacag catcgcggcg gctgcaccga gcagtaccca tgcgctgatt
    32701 tcggcgactt cgcgcagggc gcgtttttgc ctgcggctga aaaggcggtt gataatcaac
    32761 gcggcaagtg cgaaaaagaa aaaaatacgg aaaagtctgc ctatcatgtc gggaagcggt
    32821 ggaggtcgtc tgaaaaagta tgattcgtgt gcggcggatg agggtcaaac gcgtccggct
    32881 gcttgtccga ccgtgtcaaa aaatcatgcc gtttgcgata taataacgct tttcgccgca
    32941 tttgttggaa caggatatga gtcaaaatgc ctcggtgggc attgtaacgc cccaaaaaat
    33001 tccgtttgaa atgccgctgg ttttggaaaa cggtaaaact ttgccgcgtt tcgatctgat
    33061 gattgaaacc tacggcgagc tgaatgccga aaaaaataat gcggttttaa tctgtcatgc
    33121 gctgtcaggc aaccatcatg ttgcgggcag gcattcggcg gaggataaat atacgggctg
    33181 gtgggacaat atggtaggac ccggcaaacc gattgatacg gaacgttttt tcgtggtcgg
    33241 tttgaacaat ctgggcggct gtgacggcag cagcggacct ttgtcgatca atcctgaaac
    33301 gggtagggaa tacggcgcgg attttccggt ggttacggtg aaggactggg taaaatcaca
    33361 agccgcgctt gccgactatc tcggcatcgg gcaatgggcg gcggttgtcg gcggcagttt
    33421 gggcggtatg caggctttgc agtgggcgat ttcctatccc gaacgcgtgc gccatgcctt
    33481 agtgattgcg tctgcgccga aactgtccac gcaaaatatc gcgttcaacg atgtggcgcg
    33541 tcaggcgatt ttgaccgacc ccgatttcaa cgaaggacat taccgcagcc ataataccgt
    33601 tcctgcacgg ggcttgcgga ttgcccgcat gatggggcac attacctatc ttgccgaaga
    33661 cggtttgggc aaaaaattcg gacgcgattt gcgttccaac ggctatcaat acggttatgg
    33721 cgttgaattt gaagtagaat cctatctgcg ctatcagggc gacaaattcg tcgggcggtt
    33781 tgatgccaac acctatttgc tgatgaccaa agctttggac tatttcgatc cggcggcgga
    33841 tttcggcaac agcctgaccc gcgccgtgca ggatgtgcag gcaaaattct ttgtcgccag
    33901 cttcagcacc gattggcgtt tcgcgcccga acgttcgcac gaactggtca aggccctgat
    33961 tgccgcccaa aaatccgtgc agtatatcga agtcaaatcc gcacacgggc acgatgcctt
    34021 tttaatggaa gacgaagcct atatgcgtgc ggtcgccgcc tatatgaaca acgtttataa
    34081 ggaatgtcag caatgaacct gcgcgatgat ttgcaactga tttacgaccg gatacccgaa
    34141 ggcagccgcg tcttggactt gggctgcggc gacggcgaac tgcttgccgc attggtcgaa
    34201 cacaaaaaat gcagcggcta cggcatcgaa atcgacaccg acagcgtgat tgccgcgatg
    34261 tcgcgcggcg tgaatgttat ccaagccgat ttggaagaag gtttaaccgc attcaacgat
    34321 caaagttttg atgtgattgt gttgagccaa accatccaag cgatgcagaa taccgaaaaa
    34381 atcctgcgct gcctgatgcg cgttgccaaa caggcgattg tcagcttccc gaatttcggc
    34441 tactggcgca accgcgtcca aatcgcactc ggcggacata tgccggtttc cgaacgtatg
    34501 ccctaccatt ggtacgacac gcccaatatc cattggtgca cgctcaaaga ctttgatttg
    34561 ttgtgcgcca aaaacaacat ccgcgtcctc gaacgcgccg tgatgacggg caacagacag
    34621 gtcaaacatt tccccaacct tttgggcagc cttgcgtttt accgcgtcgg ctaagacagg
    34681 ttgagaacct tgtcggcaat aacaggttct tcggttctat taacaacagg ccgtctgaaa
    34741 aatgaaacgg taggttttca gacggcctct ttaatactgg aaacaataga atgaaaaaca
    34801 aaacctcatc acttctctta tggcttgccg caatgatgct gaccgcgtgt tccccgagca
    34861 aagaagataa aacgaaagaa aacggcgcat ccgccgcctc gtccacggca tccgccgctt
    34921 cgtcttccgc gccccaaacc gatttgcaac cggccgcatc cgcccctgat aacgtcaagc
    34981 aggcagaaag cgcgccgccg tcaaattgca ccgacctgca ccccgccacc ggcattgacg
    35041 atctcatgca gcaaatcgcc gaacacatcg actcggactg tctgtttgcc ctttcccatc
    35101 acgaactgga aacccgtttc ggcttacccg gcggcggcta tgacaacata cagcggctgc
    35161 tgtttcccga catccgccct gaagatcccg actaccatca gaaaatcata ctggcaattg
    35221 aagacttgcg ttacggaaag cgcacgatca gccggcaggc acaagatgcc ttgatggaac
    35281 aggaacgccg cctccgagaa gcgacgctgt tgctgataca gggcagtcaa gaaacccgcg
    35341 gacaaggcga ggagccgaaa cgcacgcgtt attttgaagt ttcggcaacc cctgcctatt
    35401 cgagccggca caacaacgga cttggcggca atttccaata catcggccaa ttgcccggct
    35461 atctgaaaat acacggagaa atgcttgaaa accaatcact cttccggctg tccaaccgtg
    35521 aacgcaatcc cgacaaaccg tttttagaca tccattttga cgaaaatggc aaaatcacgc
    35581 gtattgtcgt ttacgaaaaa aacatctact tcaatccaaa cttggggcga agataaaaaa
    35641 atgccgtctg aaacgttttc agacggcatt tttcaatgca acaaatcaag cgcgtttgcg
    35701 gaattcgccg gtgcgggtat cgatttcgat tttgtcgccg ttttcgatgt aagacatcac
    35761 ttggatttcg gtgcccccga ccaggcgcgc ggttttcatc actttgcctg aagtatcgcc
    35821 tttgacggca ggctcggtat actcgacttc gcgtacgatg atggtgggca gttctacgga
    35881 gatggggttg ccttcgtaga aggtaacttc gcattggtct tccatgccgt caacgatgaa
    35941 tttcaacgcg tcgccgatgt tgtcggcttc gatttcgtat tggttgaatt cttcgtccat
    36001 aaagacgtac atcggatcgg caaagtagct gtacgtacag tttttgcggg acaggatgac
    36061 cacgtcgaat ttgtcgtcgg ctttgtaaat ggtttcggaa gccgcgccgg tcagcaggtt
    36121 tttcagtttc atgctgactt tggcggaaga gcggccgcct ttgatgtatt cggttttttg
    36181 aacgaccata ggatcgttgc cgaccataaa tacattgccg gcgcgcagtt cttgtgctgt
    36241 tttcattgtg attttccaat caaatcggtt gtcgtaaaac gcactattct agcgtatttt
    36301 ttgatgcttt gaaacaaagg cggcgagttt ttcggatgcg gaaggctgcc cgaaaagata
    36361 acggctccaa tcctccgcgc cttgccgcca gccgttttga tgttgttgca ggatttgcca
    36421 acattcgagg cgttgcgtgg cggataaagc ccctccgccg ttgaggtcgt ccgaaagacg
    36481 gcggtgtgcc gacacggttt cgggcgtgta gaaaccgtgt gccttatccc aaaaggcgtg
    36541 gagtttgtcg agatggacat tctcgtcttg cgggtagatg tgccaaaaga agggtttgcc
    36601 ggcaagctgg gcgcgcacga aactgtcttc gccgcgtatc acggcgcagt cggcgaggtg
    36661 cagcagtttg tcgaagtcct gttgcggcac gaaagggatt ttgacgaggc ggacggatgc
    36721 cgtctgaaaa acatcgccgt cgttttgcag ggcatcttgc ggaataacgc cgctttgttt
    36781 gaggctgtcg atgatttgcg tccccgccag caacagtgtc atcgggctgc ctgcctgtcg
    36841 ccacatttcc agccactttg cccaaacatc gctccgatag ccgaaaagca gccattcggg
    36901 ggcgtttttt tcgggaagca tcagccgctg acgcagggct tcgctatcga aacggacggc
    36961 ttcgcagtaa tcgcgttcgc gtatcaaccc gccgcttttt tcgctgaaac ccataaacca
    37021 aaaatatttt tgaacacctt cctgcggcga aggcatcagg tgcagccttt cattgctttc
    37081 ctccgcgctc aaatattccc aattcagcca aagcggcttg tgtcggcgga taatgtgcag
    37141 cacattttcg ggcaggtcgc aggcaaaagt ttcgatgacg acatcgggaa caggcgcggt
    37201 atcaatatct gccgcatcgg aatgccaagt gcggacatga atatcctgat gaacgcaggg
    37261 aacatcgggc aaatcagggc aaagcgcacg caaggcggac acatcgtccg tccacaaatg
    37321 cacctgccaa ccgagttcgc ggtgcaaaac acgggcgagc cgccacgaaa cgccgatgtc
    37381 gccgaaattg tcgatgacct tgcaaaaaat ccaacagaca aaaggaggag tattcataga
    37441 aataacggaa acgggctaaa acgcgccgaa atagtggaac aaatacacaa tcagcaaaat
    37501 aatcggaata aacagcaaca tagtttgacg tttcaaatca aactcttgct tcaaatgatg
    37561 cttgatggtg tcgaaaagga cgaatttgaa caaaaacgtc gcttcgcagg caaggatgat
    37621 gccgttaatc agatgctggg gtttgtcttc gggcggattg gcggcgaaaa cggcaagcgt
    37681 cgccaagggt atcaggcaga cgacaagcca gcggacaagc aggtttcggg acattgcaag
    37741 gctttcaaaa aagcaaaacg ccattatcgc cccaagcgca cgccttgtct aacggatgcg
    37801 ccggagatgc cgtctgaagc cttgccgcag gaaaacaccg caccgccgaa cctttaaata
    37861 cgcccgagac gggcgttccc cgaaaaatcg gacgctgccg ccgatgcctg aggacaaggc
    37921 ggcagcaggc acgggcatat tgaaaacccg gcgcgtcggt ttgaaatttt atagtggatt
    37981 aacaaaaatc aggacaaggc gacgaagccg cagacagtac aaatagtacg gaaccgattc
    38041 acttggtgct tcagcacctt agagaatcat tctctttgag ctaaggcgag gcaacaccgt
    38101 actggttttt gttaatccac tatataaagg catacggtaa acacgccccc ttgccgtcgg
    38161 cgtgcttccg caaaccaaaa tataacgtcg gcacgctttc cgctataatc cgtatttttc
    38221 caaacctgaa acgttatgcc gaccccgaaa gcctttgccc tgctcgggcc gaccgccgga
    38281 ggcaaaaccg cgctcgccct caaaattgcc gaaaccctgc ctgtcgaaat catcagcctc
    38341 gattccgcgc tggtttaccg cgatatggac atcggcacgg cgaaaccgtc cgcctccgaa
    38401 cgcgcctttg tcccgcacca ccttatcgac atcattacgc ctgttcagac atacagtgcc
    38461 gcccgttttg tcgaagactg cacgcgtctg attggcgaaa tcacggcgcg tgggaaatat
    38521 ccgctgattg tcggcggcac gatgatgtat ttccgcgccc tgacccaagg tttgaacgat
    38581 ttgcccgaag ccgatgcctg cctgcgtgcc gacttggacg aacaaaaaca aatgtacggc
    38641 ttggatttcc tctaccgcac cctgcaacag gtcgatcccg aaacagcatg ccgtctgaaa
    38701 ccgaacgaca gccagcgtat cggacgcgct ttggaagttt attatctgac cggcaaaccg
    38761 atgagcgaac acctcggcag acaaagcccc catacgctcc ccttcgattt gcataccgcc
    38821 gccctgattc ccgaaaaccg cgcccgcctg catgaaaaca tcgccctgcg tttccacctg
    38881 atgcttgaac agggctttat cggcgaagta gaaaacctgc gccgccgcta tcccggcctg
    38941 accgccgact cccccgccat ccgctgcgtc ggctaccgtc aggcatggga atatctggac
    39001 ggaaaaaccg atttcccggc atttgtcgag aaaggcattg ccgccacccg ccaacttgcc
    39061 aaacgccaac tgacttggtt gcgcaaaaca cctttagact gcgttgccga cccgttttca
    39121 gacggcactt catgcacacg cctgattgag gcggcaaaac ggtttttcgg cgcataaacc
    39181 atgctgacca caccgccgct cgcccccaaa accgtcgccg ccctgcacag gctcggtatc
    39241 cgtacgctcg aagaactgcg gcaaaacggt tcggtcaaag cctttctgct gctcaaagca
    39301 tcgggtttga ccctcacgaa aagcacgctg tggcagcttg agtcgctgct gaacggcaca
    39361 ctcccgcaag aaatcacgca acacgacaaa acgcgccttt ttgccgaatt gaaaaaccat
    39421 ccgcccgtcg ccgcctttcc gccgcaggaa gaaatggaac acttcatgcg cgaagcactg
    39481 cgccaagcag aacaatccgc cgctgacggc gaaatacccg tcggggcagt catcgtttca
    39541 gacggcaaaa tcatcgcatc ggcacacaac acctgcattg ccgactgcaa tgtcagccgg
    39601 cacgccgaaa tcaacgcctt gtcacaggca ggcagcgaaa tgcaaaacta ccgccttgac
    39661 ggatgcgaca tatatatcac cctcgaaccc tgcgccatgt gcgcgtccgc actgatacag
    39721 gcacgaatca ggcgcgtgat ctacggggcg gcagaaccca aaaccggcgc ggcaggcagc
    39781 atcgtcaacc tgtttgccga caaacgcctc aatacgcaca ccgccatacg gggcggaatc
    39841 ctgcaagaag aatgccgcgc cgtgttaagc cgtttttttc aaaacaaaag aaaaggttga
    39901 acctatgacc gtactgaccg tcatcctcgc cctcgccctg atagccgtcg gcacggcggg
    39961 catcgtttac cccgccctgc ccggattggc attgatgttt gccggaacat ggctgcttgc
    40021 ctatgccggc ggctaccaaa tctacggcgc gggcgttttg tggacggtcg gactcatcag
    40081 ccttgccggc atactggcgg actatgtggc aggcatatgg gggacaaaat ataccggagc
    40141 gggcaagctc gccgttcgcg gcgcattggc cggcagcatc atcggcatat ttttctccct
    40201 tcccggacta atactcggtc cctttatcgg cgcggcggca ggcgaactga tcgaacggcg
    40261 caatatgctt caggcaggta aagcgggctt gggtacgctg ttggggcttg tcgtcggcac
    40321 ggcgttcaaa atcggctgcg ccgtatccat cttgtttatc ctgttggtga aatacatcgc
    40381 ctacctgttt taaccctttc agacggcata ccggcaaatt gctataatgc tgtctgaacc
    40441 ctttttacaa agcaccatca tggcaaacca aagacccatc tacggcttcc acgccgtcaa
    40501 cgcccgattg tggcaaaacc ccaaatccat cgtcgaactc tacatccaag aaggcaaatc
    40561 agacgcacgc acgcgcgaag tgttggaaaa ggcggcaaac gaaaacatcc gcgtatattt
    40621 tgtcgatgcc gaccgcctca acgccatcag caaaggcgcg cgccatcagg gcgtggtcgg
    40681 atttatcgat gcctccaaaa accacgtcca cctcgaagac gtattggaaa acctcagcga
    40741 accgccgctg ctgctgatac tcgacggcat caccgatccg cacaacctcg gcgcgtgcct
    40801 gcgtaccgcc gacgcaatgg gcgtacacgc cgtcatcgca ccgaaagaca aaagcgcggg
    40861 gctgaacgcc accgtcagca aagtcgcctg cggtgcggcg gaaaccgtcc cctatatcac
    40921 cgtaaccaac ctcgcccgca ccctgcgcga attgaaagaa tacggcattt ggattatcgg
    40981 caccgatatg agcggcgagt ccgaccttta ccattgcaat ctgcccgaca gcgcggcgtg
    41041 ggtaatgggc aacgaaggcg acggtatgcg ccgcctcacg cgcgaacatt gcgacatgct
    41101 ggtgtccata cccatgttcg gcacagtcga aagcatgaac gtctccgtgt ctgccggaat
    41161 ggtattaagt gaaacacgcc gacaacgcgt attaaaaaac gaaaaagtat aagcctcaaa
    41221 aacaatgccg tctgaaacag ggattctgtt tcagacggca ttcattttca aaatcagtgt
    41281 tccaagtggt cggtatcaaa cttgaccgcc gcctcggttt tatcttcggg caagaccaaa
    41341 ttcagcaaga ctgccgtaat gccgccggcg gaaatagagt tttggaacaa gacgggcagg
    41401 tttttaaaca cttccggctc aaacgcgaca cccaagccca aaccgaccga cgttgccgca
    41461 atcaccgctt cgcgcctgcg gatgccgtga ctgaccaaaa tccgcacgcc cgcaatcgca
    41521 attaagccga acatcaaaac catcgcgccg cccaacaccg gactcggaat cgtcgtaaac
    41581 gcgcgaccga caacggggaa cagacccaac agcaccaaaa tcacggcaat atatttgccc
    41641 acatgacgcg aagccacgcc ggtcatctga atcacgccgt tgttttgcgc aaacgtcgtc
    41701 agcggcagcg aacccaaagc cgtcgcaatc accgacacca agccgtcagc caacacgccg
    41761 ccgcgcaggc gtttggtgta ttcctcgcct tcaatcggct gatcggacac cattgccgtt
    41821 gcggtcaaat cgccgaccgc ctcaaacacg ctcaacaaga aaatcgcgcc cgccacaata
    41881 aacgcgtgcc agtcaaaagc aaaaccatat ttaaacggta cgggcagcgt aaccagcggc
    41941 aggttttgca gcgcggaaaa atccaccttg cccaaaaaca gcgcgacgat atagccggca
    42001 atcagcccga ccgcaatgcc gctcatgcgc aacagcgggt ttttcatgca gttgaacacc
    42061 aacacaatca gcaacaccag cgatgccagc cccaagtttt ccatcgagcc gaacgtgccg
    42121 tccgccttcg cgccgaagcc gccgccgaaa tcggtaatgc cgacgtgtac caaactcaaa
    42181 ccaatgagca tcacgaccac gccgctgacc gtcggcgtaa tcaccttttt caaatacggc
    42241 agaagccacg ccgagaaaca caccaaaaac gcgccgacaa acgatacgcc caagagcgtc
    42301 gaaatcatcg catccttagt caaaccgccc tctttcatcc ccgcgccgag cgcaatcatg
    42361 acagtaacga acgagaaatt caccgactgg atggacagca tccccgaacc gaccggcccg
    42421 aagcggttga cctgcaaata agtgccgaca cccgacgcaa ccatcgccat cgacacgaga
    42481 tacgccgtca tctccaccgg caattccagc gcgccgccca caatcagcgc gggcgtaatc
    42541 atcggcacaa aaatcgccaa aagatgggta accgcgctca agagcgcatt accgaacggc
    42601 ggcctatcct ccaaaccgta caccaaatca ggcgcatccg cacctttgcc caactgaccg
    42661 ctcattgtat ttccttattt taaaggttga aaaatccaag cgcggattgt agcgaatctg
    42721 ccgcacggct tcaatttgca tttcagacgg catccgtctg gcccacacgt ttaccacatt
    42781 ttatagtgga ttaacaaaaa tcaggacaag gcggcgagcc gcagacagta cagatagtac
    42841 ggaaccgatt cacttggtgc ttaagcacct tagagaatcg ttctctttga gctaaggcga
    42901 ggcaacaccg tactggtttt tgttaatcca ctataaatcc gacggcaccg gcataaaccg
    42961 gcgaatataa taaaatgatt tcaaacagga aaaatcaatt tccaccatac ctttcggcgc
    43021 aaacgcccca catccgcaac ccgccggatt tcacacctgc ggtaaagtag gctacaatcg
    43081 gaagcattac cgccccctac accacaactg ataaaaggtt ctgtatgtta caaggtagcc
    43141 tggttgccct gattaccccg atgaatcaag acggcagcat ccattacgaa caactccgcg
    43201 acttaatcga ctggcacatt gaaaacggca cggacggcat cgttgccgtc ggcacgacag
    43261 gcgaatccgc caccctctcc gtcgaagaac acaccgccgt catcgaagcc gtcgtcaaac
    43321 acgttgccaa acgcgtcccc gtcatcgccg gcacaggggc aaacaacacc gtcgaagcca
    43381 tcgccctttc ccaagccgcc gaaaaagccg gggcggacta caccctctcc gtcgtcccct
    43441 attacaacaa accttcgcaa gaaggcatgt accgccattt caaagccgtt gccgaagccg
    43501 ccgcgattcc gatgatactc tacaacgttc ccggcagaac cgtcgtcagc atgaacaacg
    43561 aaaccatcct gcgccttgcc gaaatcccca acatcgtcgg cgtgaaagaa gccagcggca
    43621 acatcggcag caacatcgaa ctcatcaacc gcgcgcccga aggcttcgtc gtcctgtccg
    43681 gcgacgacca caccgcccta ccattcatgc tctgcggcgg acacggcgtt attaccgtag
    43741 ccgccaacgc cgcacccaag ctctttgccg atatgtgccg cgccgcgctt caaggcgata
    43801 tcgccctcgc acgcgagctc aacaaccggc tgattccgat ttacgacacc atgttctgcg
    43861 aacccagccc cgccgcaccg aaatgggcgg tatccgccct ggtcagatgc gaaccgcacg
    43921 tccgcctgcc gctcgttccg ctgacggaag gcggacaggc aaaagtccgc gccgccctga
    43981 aagcctcagg acaactttaa atatagccat gccgtccgaa ccgttcggac ggcataacgc
    44041 aacaaacact ttaatatcca tcacacagga tgacacgatg acccatatca aacccgtcat
    44101 tgccgcgctc gcactcatcg ggcttgccgc ctgctccggc agcaaaaccg aacagcccaa
    44161 gctcgactac caaagccggt cgcaccgcct gatcaaactt gaagtcccac ctgatttgaa
    44221 caaccccgac caaggcaacc tctaccgcct gcctgccggt tcgggcgccg tccgcgccag
    44281 cgatttggaa aaacgccgca cacccgccgt ccaacagcct gccgatgccg aagtattgaa
    44341 aagcgtcaaa ggtgtccgcc tcgagcgcga cggcagccaa cgctggcttg tcgttgacgg
    44401 caaatccccc gccgaaatct ggccgctcct gaaagccttc tggcaggaaa acggcttcga
    44461 catcaaatcc gaagaacccg ccatcggaca aatggaaacc gagtgggcgg aaaaccgtgc
    44521 caaaatcccc caagacagct tgcgccgcct attcgacaca gtcggtttgg gcggcatcta
    44581 ctccaccggc gagcgcgaca aattcatcgt ccgtatcgaa cagggcaaaa acggcgtttc
    44641 cgacatcttc ttcgcccaca aagccatgaa agaagtgtac ggcggcaaag acaaagacac
    44701 gaccatgtgg cagccttccg cttccgaccc caaccttgag gccgctttcc tgacgcgctt
    44761 tatgcaatat ttgggcgttg acggacagca ggcggaaaac gcatcggcaa aaaaacctac
    44821 ccttcccgcc gccaacgaaa tggcgcgtat cgaaggcaaa agcctgattg tctttggcga
    44881 ctacggcaga aactggcggc gcaccgcgct cgccctcgac cgcatcgggc tgaccgtcgt
    44941 cggtcaaaac accgaacgcc acgctttcct ggttcaaaaa gccccgaacg aaagcaatgc
    45001 agttaccgaa caaaaacccg gcctgttcaa acgcctgctg ggcaaaggca aagcggagaa
    45061 acctgccgaa cagccggaac tgattgtcta tgcagaacct gtcgccaacg gctcgcgcat
    45121 cgtcctgctc aacaaagacg gcagcgcata tgccggcaaa gacgcatccg cactgttggg
    45181 caaactccat tccgaactgc gttaaaccgt tcaaaacaaa agcagcttcc aaacagaagc
    45241 tgcttttttg tgcggaactt tttacaacaa ccgaggaaaa atatcctcaa ccgcctgaac
    45301 caacgcatcc gccaaaggcg gatcgaacgc acgatgccct gcctgaacca ccctcaattc
    45361 cgcttccgga aaggctttcg acagcgccca cgcactctgc atcggcgtac acaaatcata
    45421 ccgcccctgt acgataacgg tcgggatatg ccgtattttg ccgatattgt tcaaaatcgc
    45481 cctatcgccc tgcaaccagc cgccgttgac aaaataatgg ttttccaaac gcgcgattgc
    45541 cagcgaggca taagcatctt catccacttc ctccggctcg aaacggatca gatagctttc
    45601 ccaatccgcc caagccttcg cggcagacag acacacttct tcatcttgat gaaacagcaa
    45661 tccgtgatac gcctcaatca gccggttccg ccgattttca gcaatcggcg cgacaaattt
    45721 ttgccattgt tccggataaa tccggctcac accgcccgcc tcgttcagcc acaccgtttc
    45781 agacggtctg cacaaaaata tcccgcgcaa caccaatccc tttacccgtt ccggatgggt
    45841 ttgggcataa gccagcgaca aagtgctgcc ccacgaaccg ccgaacacca gccatttccc
    45901 gatacccagc atttcacgga ctttttcaat atccgccacc aaatcccaag tcgtattgtc
    45961 ttccgcacaa gcatacgggc gcgaacgtcc gcaaccgcgc tggtcgatga tgacgatgcg
    46021 gaacacatcg ggattgaaaa aaccccgaca ttcaggcgaa gcccccgcgc ccggcccgcc
    46081 atgtaaaaaa ataaccggca caccgtcagg attacccgac tcttcccaat aaatttgatg
    46141 aatttcagat acctgcaagt atccgctatg aaaaggctgt tttatctcat acatcatctt
    46201 ctcccatatt gcttctgaca atccgaacat cccgacacgc gacatcccgc acaaaacaca
    46261 agccgccccg aaaggcaaat gccgaaaaat cgcgatatag tggattaaca aaaatcagga
    46321 caaggcgacg aagccgcaga cagtacaaat aatacggaac cgattcactc gtgcttcagc
    46381 accttagaga atcattctct ttgagctaag gcgaggcaac gccgtac
//
LOCUS       DAWFLT010000006        43881 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWFLT010000006 DAWFLT010000000
VERSION     DAWFLT010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 43881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43881
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.006"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..713
                     /locus_tag="WGR74_000295"
     CDS             <1..713
                     /locus_tag="WGR74_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0904793.1"
                     /translation="HQFAQSQPLTKTDAKDAKMPAFFAQMTAQKEDSQTMPYQPPTEA
                     EEVLEALVNRRNQLVDMRTAEKNRLHQVHETQVGSVKQLIAHFDRLIDELDKQIDDHT
                     HTHFDGKAQVAEQIKGIGSITTATLMAMLPELRRLSHKRIAGLAGIAPHPRESGETKF
                     KSRCFGGRSAVRKALHMATVAATRFEPLIRDFYQRLLSEGKPYKAAVTACMRKLLTIS
                     NARMRDYFAENDTAENGI"
     gene            770..1843
                     /locus_tag="WGR74_000296"
     CDS             770..1843
                     /locus_tag="WGR74_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ0904794.1"
                     /translation="MRPFFVGATVLAILGALVFFISPGAVVLHRQIFLELMLPAAYGG
                     FLTAALLDWTGFSGNLKPVATLMAALLLAASAILPFSPQTASFFVAAYWLVLLLFCAR
                     LIWLDRNTDNFALLMLLAAFTVFQTAYAVSGDLNLLRAQVHLNMAAVMFVSVRVSILL
                     GAEALKECRLKDPVFIPNVVYKNIAITFLLLHAAAELWLSAQTAGFTSLAVGFILLAK
                     LRELHHHELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGMMGSV
                     MMVWLTAGLWHSGFTKLDYPKLCRIAVLLLFMAAVSRAFLMNVNPIFFITVPAILTAA
                     VFVLYLLTFVPIFRANAFTDDPE"
     gene            complement(1889..4078)
                     /locus_tag="WGR74_000297"
     CDS             complement(1889..4078)
                     /locus_tag="WGR74_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ0904795.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILSVRTAFSDEPLLPESWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPLARFNKKAALWDALLSGGMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGKRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHAAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPYKCPDCGNQDLTAVGHGTQRVEETLRAFLPRAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYVVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADALEFLNAAKETLAPLLPASVSQFGAAPMLMVRLA
                     ERERAQIFLESPSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            4217..4999
                     /locus_tag="WGR74_000298"
     CDS             4217..4999
                     /locus_tag="WGR74_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ0904796.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            complement(5302..7236)
                     /locus_tag="WGR74_000299"
     CDS             complement(5302..7236)
                     /locus_tag="WGR74_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ0904797.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIQEKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(7302..8480)
                     /locus_tag="WGR74_000300"
     CDS             complement(7302..8480)
                     /locus_tag="WGR74_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ0904798.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMKDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            8706..9092
                     /locus_tag="WGR74_000301"
     CDS             8706..9092
                     /locus_tag="WGR74_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0904799.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVR
                     TTLLHNAQTMERYYRQKGTFQTYDQTKLKQNKYFKITLSKSPDHFTLTADPDPMTNEG
                     ETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            9377..10423
                     /gene="adhP"
                     /locus_tag="WGR74_000302"
     CDS             9377..10423
                     /gene="adhP"
                     /locus_tag="WGR74_000302"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ0904800.1"
                     /translation="MKMQAVVVNKNVAGDVEVVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SGVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(10774..14259)
                     /gene="smc"
                     /locus_tag="WGR74_000303"
     CDS             complement(10774..14259)
                     /gene="smc"
                     /locus_tag="WGR74_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ0904801.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEVSAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELGNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQMQRIRQE
                     QQQIRVQLEENELYVEEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASDRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLQARSVPHGFVPPAPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELDNLAPELSTAEAAFKQAE
                     ATVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIFTLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIARLEQQTLDWQERQQELVLAYETEFQNDEQHIKLEELS
                     EAVQTLDEEYIAVQDKLAQIQAQGREQYARVQTLQTQLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLTQQIEALGAVNLAALQELEEVR
                     ERDGYYRSQSEDVQAAITLLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAESV"
     gene            14508..15230
                     /gene="lpxH"
                     /locus_tag="WGR74_000304"
     CDS             14508..15230
                     /gene="lpxH"
                     /locus_tag="WGR74_000304"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ0904802.1"
                     /translation="MKPAYFISDLHLSEKQPELTALLLRFLRSSAARQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQNFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLATKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFGAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKC"
     gene            15500..17086
                     /locus_tag="WGR74_000305"
     CDS             15500..17086
                     /locus_tag="WGR74_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0904803.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(17255..17458)
                     /locus_tag="WGR74_000306"
     CDS             complement(17255..17458)
                     /locus_tag="WGR74_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ0904804.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            17574..18071
                     /locus_tag="WGR74_000307"
     CDS             17574..18071
                     /locus_tag="WGR74_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ0904805.1"
                     /translation="MTALTLPEDIRQQEPSVLLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGETIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHILSNWETFA
                     HIGQA"
     gene            18153..19346
                     /locus_tag="WGR74_000308"
     CDS             18153..19346
                     /locus_tag="WGR74_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0904806.1"
                     /translation="MFFKHIEAALADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PIVKAVKEAEKHLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVGLLKAKG
                     ASQNFDFIIKQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(19750..20685)
                     /gene="hemC"
                     /locus_tag="WGR74_000309"
     CDS             complement(19750..20685)
                     /gene="hemC"
                     /locus_tag="WGR74_000309"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ0904807.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYARLEEMPKGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLKLDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLQADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            20804..21319
                     /gene="ybeY"
                     /locus_tag="WGR74_000310"
     CDS             20804..21319
                     /gene="ybeY"
                     /locus_tag="WGR74_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ0904808.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGVLHLMGYDHIEDDEAEIMEAEEIRLMLAA
                     GFPNPYQEDEY"
     gene            21321..22145
                     /locus_tag="WGR74_000311"
     CDS             21321..22145
                     /locus_tag="WGR74_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEZ0904809.1"
                     /translation="MDGTQSKPKFFERLISRLAGEPDSAEDVLTLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSVLRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(22274..23653)
                     /gene="nhaC"
                     /locus_tag="WGR74_000312"
     CDS             complement(22274..23653)
                     /gene="nhaC"
                     /locus_tag="WGR74_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ0904810.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPNVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVILALMRINAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAIRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDRLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(23944..25167)
                     /locus_tag="WGR74_000313"
     CDS             complement(23944..25167)
                     /locus_tag="WGR74_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ0904811.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
     gene            25757..26164
                     /locus_tag="WGR74_000314"
     CDS             25757..26164
                     /locus_tag="WGR74_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0904812.1"
                     /translation="MMPAYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYGALLC
                     AGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLAFAWLPVVGDALPLAAG
                     WLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            26210..26839
                     /gene="nth"
                     /locus_tag="WGR74_000315"
     CDS             26210..26839
                     /gene="nth"
                     /locus_tag="WGR74_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ0904813.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYKG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            26977..28476
                     /locus_tag="WGR74_000316"
     CDS             26977..28476
                     /locus_tag="WGR74_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ0904814.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIKHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSSNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLIMRRGNTLFIALNLQ"
     gene            28678..30198
                     /locus_tag="WGR74_000317"
     CDS             28678..30198
                     /locus_tag="WGR74_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ0904815.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDGSWGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISMVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            30255..31340
                     /gene="nagZ"
                     /locus_tag="WGR74_000318"
     CDS             30255..31340
                     /gene="nagZ"
                     /locus_tag="WGR74_000318"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ0904816.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAVGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGQCAVIGNRSFHHNPEAVAHLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDRRSLSELEAADLAPFHIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELREDFRIPDNPALAQRWQYMANTLGSAAAQAVIQTAD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            31421..31497
                     /locus_tag="WGR74_000319"
     tRNA            31421..31497
                     /locus_tag="WGR74_000319"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31455..31457,aa:Pro,seq:cgg)
     gene            complement(32018..32656)
                     /locus_tag="WGR74_000320"
     CDS             complement(32018..32656)
                     /locus_tag="WGR74_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ0904817.1"
                     /translation="MKKISVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGRLKAYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYEKSCIETADEVRIVADGDVVAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(32653..33024)
                     /locus_tag="WGR74_000321"
     CDS             complement(32653..33024)
                     /locus_tag="WGR74_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ0904818.1"
                     /translation="MKLLFIPLVLFVAVEHFYIAWLEMTQIPSEKAAETFKLPYEFME
                     QNRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            complement(33293..33715)
                     /gene="queD"
                     /locus_tag="WGR74_000322"
     CDS             complement(33293..33715)
                     /gene="queD"
                     /locus_tag="WGR74_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ0904819.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGIIKGG
                     AKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNSRECQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            complement(33722..34240)
                     /locus_tag="WGR74_000323"
     CDS             complement(33722..34240)
                     /locus_tag="WGR74_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ0904820.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTKMPSENAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            complement(34268..34927)
                     /gene="queC"
                     /locus_tag="WGR74_000324"
     CDS             complement(34268..34927)
                     /gene="queC"
                     /locus_tag="WGR74_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ0904821.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            complement(35112..35306)
                     /locus_tag="WGR74_000325"
                     /pseudo
     CDS             complement(35112..35306)
                     /locus_tag="WGR74_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            35471..36697
                     /locus_tag="WGR74_000326"
     CDS             35471..36697
                     /locus_tag="WGR74_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ0904822.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(36718..36999)
                     /locus_tag="WGR74_000327"
     CDS             complement(36718..36999)
                     /locus_tag="WGR74_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0904823.1"
                     /translation="MIHEEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDEATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            complement(37078..37212)
                     /locus_tag="WGR74_000328"
                     /pseudo
     CDS             complement(37078..37212)
                     /locus_tag="WGR74_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(37211..37669)
                     /locus_tag="WGR74_000329"
     CDS             complement(37211..37669)
                     /locus_tag="WGR74_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904824.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
     gene            complement(38233..38436)
                     /locus_tag="WGR74_000330"
     CDS             complement(38233..38436)
                     /locus_tag="WGR74_000330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904825.1"
                     /translation="MLSLEIQQEGFTIGGGLVLGGCAGAHLARKEPLILTGKTGAGAS
                     AIANASIGYQWTVNLSKPKEGAK"
     gene            complement(39000..39935)
                     /locus_tag="WGR74_000331"
     CDS             complement(39000..39935)
                     /locus_tag="WGR74_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ0904826.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCLDSIDYVDERKGVP
                     LAAMQHIFMDVRAAASHAYLFEHDLKKFKQYAYVAGKLGVLLSVNSTDPEPFFFPCDM
                     LNIQNPMFLMLMSDSPQLREFLVRNIDNIANDTEAFINRYDLNRHMIYNTLLMVEGKQ
                     LDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMA
                     AKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDQEISPRDLIVYDPLPADEYQDIFDF
                     MKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
     gene            complement(39938..40558)
                     /locus_tag="WGR74_000332"
     CDS             complement(39938..40558)
                     /locus_tag="WGR74_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904827.1"
                     /translation="MLGVVPGIGESIQAYKVAKAAKNLQGMKKALDKAATVATAQGYV
                     SKTKIKIGQTELRVTAATDKQLLKAIGEGRDTTGKMTEQLFDSLAKQNGFRVLSGGKY
                     GGNNGFDHVWQAADGSVVLIVESKQIRNGTVQLNPNGAGGYTQMSEDWIRQVLDQLPD
                     GSPAKAAVFKANKNGTLKTAIAGVDRQTGKAVILPVKVPSKTNIRR"
     gene            complement(40652..41014)
                     /locus_tag="WGR74_000333"
                     /pseudo
     CDS             complement(40652..41014)
                     /locus_tag="WGR74_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(41110..41382)
                     /locus_tag="WGR74_000334"
     CDS             complement(41110..41382)
                     /locus_tag="WGR74_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904828.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNDMD"
     gene            complement(41570..42058)
                     /locus_tag="WGR74_000335"
     CDS             complement(41570..42058)
                     /locus_tag="WGR74_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904829.1"
                     /translation="MKKDIFYCEQWSYGYKRLHKPFSEKQAEEKHLKGELYTAVIGSA
                     TQPEYVITLREEVGFFSVNFFDKFGRDYLTHQFQKYSNSNYYFLSMAVWRDYITLESH
                     DLAEGYTYFFNENTDDCYVLKQDFINNERYEKTELYSQKDKVILFPKFGEYDLVLNPD
                     II"
     gene            complement(42055..>43881)
                     /locus_tag="WGR74_000336"
     CDS             complement(42055..>43881)
                     /locus_tag="WGR74_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0904830.1"
                     /translation="IKSSIHTETVSSSKSTLWQKQAGRGSNIETLQLPSFTGPVAPVL
                     SAPGGYIVDIPKGNLKTQIETLTKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEG
                     MTPAAAAVVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGAAAGTAAGTGVAAGTAATT
                     GVAAGTSAAAITTAAGKAALASLASQAAVSLINNKGDINHTLKELGKSSTVRQAATAA
                     VTAGVLQGISGLNTQAAEAVSKHFHSPAAGKLTANLINSTAAASVHTAINGGSLKDNL
                     GDAALGAIVSTVHGEVASKIKFNLSEDYIAHKIAHAVAGCASAVANKGKCRDGAIGAA
                     VGEMVGETLLDGRDVGKLSPQERQKVIAYSQIIAGSAVALVKGDVNTAANAATVAVEN
                     NSLLARRRVNIRWTPRQELEHEYAILEIQAITNQIRRLDPKFNGIAILRTPGEPWTRH
                     DVQTYRQYYNQLRESRGFAVEPIYRIRINNGNEFNRIMSSKYPYNELYVANPKSATGY
                     FRVDSYDPATREIISRKFTQFSQIQESTGIGYIKEAVRKYSPGTVISNVPSTPTTIRG
                     RKLEGKLILEVPAQVNPIPQSVLRAAQEENVIIRDTTGRIYK"
BASE COUNT        10228 a        12008 c        10982 g        10663 t
ORIGIN      
        1 cgcatcagtt tgcccaatcg cagccgctga ccaaaaccga cgccaaagat gccaaaatgc
       61 ccgccttctt cgcacagatg acggcacaga aagaagattc gcaaaccatg ccctaccaac
      121 cgcccaccga agcggaagaa gtgttggaag ccttggttaa ccgccgcaac caactggtgg
      181 atatgcggac tgccgagaaa aaccgtctgc atcaggttca cgaaacgcaa gtcggaagcg
      241 tcaaacaact gattgcccat tttgaccggc tgattgacga attggacaaa caaatcgacg
      301 accacaccca cacgcatttt gacggcaaag cccaagtggc ggagcaaatc aaaggcatcg
      361 gttcgataac gacggctacg ctgatggcga tgctacccga attgaggcgg ctgtcgcaca
      421 aacggatagc gggtttggcc ggcattgccc cgcacccgag ggagagcggg gaaaccaaat
      481 tcaaaagccg ctgctttggc ggaaggtctg cggtgcgtaa ggcactgcat atggctaccg
      541 tggcagcgac acgttttgaa ccgcttattc gggatttcta ccaacgcctg ctgtccgagg
      601 gtaagccgta taaggctgcc gttacggcat gtatgcgcaa actgctgacg atatcgaatg
      661 cccggatgcg tgattatttt gccgaaaacg ataccgccga aaacggtatc taaacggctt
      721 gatttgagtt ttggtatttt tgcccgacgg ggtgaaaaat acagttgcta tgcgcccgtt
      781 tttcgtcggc gcgacagtgc ttgccatact cggcgcgttg gtgtttttca tcagccccgg
      841 tgccgtcgtc ctgcaccgcc aaatcttctt agaactcatg ctacctgcgg catatggtgg
      901 tttcctgact gcggctttgt tggactggac gggtttttcg ggtaacctga aacctgtcgc
      961 gactttgatg gcggcattat tgctcgccgc atccgctata ctgccctttt cgccgcaaac
     1021 tgcctcgttt ttcgtcgccg cctattggct ggtgttgctg ctgttctgcg cccggctgat
     1081 ttggctagac cgaaacaccg acaacttcgc cctgctaatg ttacttgccg cgttcactgt
     1141 ttttcagacg gcatatgccg tcagcggcga tttgaacctg ttgcgcgcgc aagtgcatct
     1201 aaatatggcg gcggtgatgt tcgtatccgt gcgcgtcagt attcttttgg gcgcggaagc
     1261 cctgaaagaa tgccgtctga aagacccagt attcatcccc aatgtcgtct ataaaaacat
     1321 cgccattacc ttcctgctcc tgcacgccgc cgccgaactt tggctgtctg cgcaaaccgc
     1381 cggttttacc tcgctcgccg tcggctttat cctgcttgcc aagctgcgtg agcttcacca
     1441 tcacgaactc ctgcgcaaac actacgtccg cacttattac ctgctccaac tctttgccgc
     1501 cgcaggctat ttgtggacag gcgcggcgaa attacaaaac ctgcccgcct ccgcgcccct
     1561 gcacctgatt accctcggtg gcatgatggg cagcgtgatg atggtgtggc tgactgccgg
     1621 actgtggcac agcggcttta ccaagctcga ctacccgaaa ctctgccgca tcgccgtcct
     1681 cctgctcttt atggctgccg tctcgcgtgc tttcttgatg aacgtgaatc caatattctt
     1741 catcaccgtc cccgcgattc tgaccgccgc cgtgttcgtg ctttacctgc tgacattcgt
     1801 accgatcttt cgggcgaacg cgtttacaga cgatccggaa taaaaatgcc gtctgaaacg
     1861 gtttcagacg gcatcgtatt gccgataatc aagcctcctg cggatcgaca tccaccgacc
     1921 atctgatttt gccgtccctg ttttgctgca acacctgcac ccacaaactc acggcacggt
     1981 gcaaatcctg tcgggacggg gattcgagga aaatttgcgc gcgttcgcgt tcggcgaggc
     2041 gcaccatcag catgggggcg gcgccaaact gggaaacgct tgcgggcaac agcggggcga
     2101 gggtttcttt ggcggcattg agaaattcca gcgcatcggc aacacgcggc gcgtccgcgc
     2161 ggacggcggt ctgaaaaccg aagggcggca tggcgaacat ttgccgttcg ttcaattcgt
     2221 tttcggcaaa cacgacgtag tcctgcgctt tgacggcggc gaagacggga tgttcgggca
     2281 gttgggtctg tatcagcacc ttgccgggct tgtcggcgcg ccccgccctg ccggacacct
     2341 gcatcagctc ggcaaacagc ctttccggcg cgcggaaatc cgcgctgtac aggctgccgt
     2401 cggcgttcaa tacgataacg aggttgagcc gcgcgaaatc atgacctttg gcgagcatct
     2461 gcgtgccgac cagaatatcg atttcgttgt cggcgatgcg gcggtacaaa tccgcccagt
     2521 cgtttttgtg cgccgtgctg tccctgtcga cgcggacgac ggcggccctg ggcaggaagg
     2581 cgcgcagggt ttcttcgacg cgctgcgtgc cgtggccgac ggcggtcagg tcttggttgc
     2641 cgcagtcggg gcatttgtac gggatgggtt cgcggtggtc gcagtggtgg cagcgcagtt
     2701 ggcgggcgcg ttggtgcagc accattttgg cggagcagtt cgggcagccg aaggtatgac
     2761 cgcagtcgcc gcaaaacagc gcgggcgcga agccgcgccg gttaaggtac accagcgaca
     2821 taccgcctgc ttcaaagttc tgtttcaaaa gctgcaaggc ttgcggcgag aagccgttgt
     2881 cgagtttcag acggcctacg ttgagaatgt ccacttgcgg cagttgcgcg gcggcatggg
     2941 cgcgttcggt cagttgcagc aggcggtacg cgccactttg cgccttgtgc cagctctcca
     3001 agctgggcgt ggcactgccc aacacgacgg ggcagccgcc ctgcttcgcc cgccacaccg
     3061 ccaaatcgcg ggcatgatag cgcaattcgt tgtcttgttt gaacgagccg tcgtgttcct
     3121 catcgaccac aatcagcccg acatcatcca tcggcgtgaa cactgccagc cgcgtgccga
     3181 tcaccagctt cgcctgccct aacatcgcgc gcagataatc ctgcgtgcgc ttgcctgccg
     3241 ccatctgact gtgcaacacg gcggtcggca cgtcggcaaa acggttttcc acccgcttca
     3301 aaagctgcgg cgtgaggttg atttcgggca acagaaacaa cacctgccgc ccctgagcca
     3361 acactttcgc catcgcatca aaatacacct cggtcttgcc gctgccggtg atgccgtaca
     3421 gcagaaacgg ctggaagcgt cccaatgccg tctgaatggc atcggaagcc ttctgctggt
     3481 cagcatttaa tacaaattca gagtgcgaag cctgcccatt acacgacctt aaaacgggct
     3541 tcgccgcttc cgtcgtttca atccagcctt gttcagccca atcctcaatc aatttcgccg
     3601 cctgcgcgtt tacctgcttc aacgccgcca tcgtcatccc gcccgacagc agtgcgtccc
     3661 acagagccgc ttttttgttg aaccgcgcca gcggcggcgt ttgcgccctg cccgcttcgt
     3721 tcagcgcgta aaacaacggc ggctgcggca tttccaccgc gcgcgtttcc ttcaaaccct
     3781 gcggcagcgc ggcaaacacc gcctgccccg tcggatagtg gtaataacgc gacgtaaacg
     3841 ccaacaaatc acgccagctt tcgggcagca gcggctcgtc cgaaaaggcc gtccgaacgc
     3901 tcaaaatccg cgccgtatcc atatcgggcg caatatccgt ttcccacacc atcccgacca
     3961 cggttttatt gcggaaaggc acaagcaccc gcgttcccgg aggaagcgga tcggaatggg
     4021 aataagtcaa aaggccgtct gaaagcggca cgtttacggc gatgcggtgg tagatcatac
     4081 aggcaggcaa atcatttttt tgcgtatttt aacaggcgga agctattttc agacgataaa
     4141 ttgagcctag gcgcaatgca ggcgcaacag agatttgata caattccgct tttccaacac
     4201 gcgcaatcat ctttatatga aaaccaagtt aatcaaaatc ttgaccccct ttaccgtcct
     4261 cccactgctg gcttgcgggc aaacgcccgt ttccaatgcc aacgccgaac ccgccgtcaa
     4321 agccgagtcc gccggcaaat ccgttgccgc ctctttgaaa gcgcgtttgg aaaaaaccta
     4381 ttccgcccaa gatttgaaag tgttgagcgt cagcgaaaca ccggtcaaag gcatttacga
     4441 agtcgtcgtc agcggcaggc agattatcta caccgatgcc gaaggcggct atatgttcgt
     4501 cggcgaactc atcaacatcg acacgcgcaa aaacctgacc gaagaacgcg ccgccgattt
     4561 gaacaaaatc gacttcgcct ccctgccttt ggacaaagcc atcaaagaag tgcgcggcaa
     4621 cggcaagctg aaagtcgccg tcttctccga ccccgattgt ccgttctgca aacgcttgga
     4681 acacgagttt gaaaaaatga ccgacgtgac ggtttacagc tttatgatgc ccattgccgg
     4741 cctgcacccc gatgccgcgc gcaaggcgca aatcttatgg tgtcagcccg accgcgccaa
     4801 agcgtggacg gattggatgc gtaaaggcaa attcccggtc ggcggcagca tctgcgacaa
     4861 tcccgtcgcg gaaaccactt ccttgggcga acaattcggc ttcaacggca cgccgaccct
     4921 cgtcttcccc aacgggcgca gccaaagcgg ctacagcccg atgccccaac tggaggaaat
     4981 catccgcaaa aatcaataat ccatccgcaa aacaaaaggc ttggaaaatt ccaagccttt
     5041 tttgcgggaa atgccgtctg aaaaccaatc cgcactgaaa gttatagtgg attaaattta
     5101 aaccagtaca gcaagccaac aaacacatcc taacccattg aatgattgaa aataataaac
     5161 tgaacaatac gaaacacacc gccgtcattc ccacgcaagt gggaatctag aacgtagaat
     5221 ctaagaaacc gtttttgtcc aataagtttc tgctccaaaa tgaccggtcc tgcagcttca
     5281 gacggcatct ttgtgccaac ctcaatcctg cgccaatgcg tctatcggat tgagtttggc
     5341 tgctttattg gcaggcataa agccgaacgc gatgccgatt ccggtcgaac aggcgaccgc
     5401 cccgataacg gatgccgccg aaatgtccat cgggaagtcg gttacaaaat gattgaacac
     5461 gaggctgacg gcggcggaca aacccacgcc gaccaaaccg ccgatgacgc agattaacac
     5521 cgcctcaatc aaaaactgct gcaagatatt gccgcgccgc gcgccgattg ccatccgtat
     5581 gccgatttct ttggtgcgct cggtaacgga caccagcatg atgttcatca cgccgatgcc
     5641 gccgactacc aatgaaatca gggcgatgga ggaaatcagc agcttcatcg taccggtggt
     5701 gctttcgact atctgcctga tgctgtcgct gttgttcatg aagaaatctt ccgtgccgtg
     5761 ccgcgctttg agcagatcgg tcagcccttt ttcggcaacc tgggtattgg cattgtcttt
     5821 gattttgacg gtgatggagt tggtgtggct ctcgcctgtg atttggtgca tcaccgtcgt
     5881 atagggcgac caaagcatca gcacgtcgga attgccgaaa gcgttttcgt cttttttcat
     5941 cacgccgatg acggtcaagg ggcgtttcct gaacaaaatg gttttaccca acggatccga
     6001 gtccgcaaag agtttgtctt tgacattttg gtcgatgacg acgacctgcg cgtcttcttt
     6061 cacatcgttt tcgtcaaaca gccgccccgt ttccagcttc agtccgcgca cgtcgaaata
     6121 ttgttcgccc acgccgtaca aagaagcggt caggtcggta ttgcggtaag tcagcgtgcc
     6181 gccgctcgaa gtcatgggcg tggcggaagc aacgtagctt tgtttggcga tgatttttgc
     6241 gtcgtctatg gtcagggttt taatcctgcc gctgcgcctg tcgccgaagc cgcgccccgg
     6301 gaagatgctg atggtgttcg tccctatcga actgatgtct tcaaggattt ttttctgcga
     6361 accattgccc aatgcgacga cggagacaac cgaagcgata ccgatgatga tgccgagcat
     6421 cgtcagaagc gaacgcattt tgtgcgccaa tactgcttgc accgacattc tgaaggcttc
     6481 gacaaactgg tcgtaataaa acgaccacga ggctttttcc tgaatcctcc cgacattgct
     6541 tgcggggatt tctggatttt tcgaggtgtc ggaaatgatt tcgccgtccc gaatttcgat
     6601 gacgcggttg gcattggcgg cgatgccggg gtcgtgcgtg accataatga cggtatgccc
     6661 ggcttcgtgc agcttatgga tgatttccat cacgtttttg ccgctggcgg tatcgagcgc
     6721 gccggtcggc tcgtcggcga agatgatttc tccgccgttc atcagggcgc gggcgatgga
     6781 gacgcgctgc tgctgtccgc ccgagagttc gccgggcttg ttgccctctt tgcttgccaa
     6841 acccaaatcc tgcaagagtt tgtccgcccg cgcggaacgc tctttgccgc ccgcgcccat
     6901 atagacggct ggcagcgcga cgttatccct tgcggtcagc gagcttaaga ggttgtagcg
     6961 ttggaagatg aaaccgaagc gttcgcggcg caatgccgcc agctcgtcag gctgcatttt
     7021 ggcagtttcg atgccgtcga ttcggtacga accggaaccg gcggtatcca aacagccgag
     7081 tatgttcatc agcgtggact tgcccgaacc ggactgcccg atgatggcga caaaatcgcc
     7141 cttctctatc gacaggctga tgtctttcaa aatatggacg cggttctcgc cgctgccgaa
     7201 atagcggttg atgtttttac attcgatcaa gctcataata ttccgtaaat caataacaaa
     7261 tgccgtctga aaccgtttcc gtgttcagac ggcatattcg tttatcggcg cggcgggccg
     7321 cctagggcgc gttcgccgct ttcctgctgc tcggcggcgg ttatttcgga gatgaccact
     7381 ttgtccccct ctttcaaacc gcttttcact tcggtattca tactgtcttt cataccggtc
     7441 cggatttcgc gttccgccgc cttgccgtcc gcacccaaca cgcgcacaaa cgccttgccg
     7501 ccgcgatttt tcacggtcag cgacggaata atcagcacat ttttcacacc gtcgatttca
     7561 accgtattct gcgtcgtcat ccccgtggcg agtttgccgt ccggattggg cacaaacgaa
     7621 cgggcataat aatagaccgc attggaagcc gtatccgtac tgctgttgta gccgcccgac
     7681 gacatcgtgg tcagcccggg gtcgacgctg tcgagcttcg ccttaatcgg cgtatccggt
     7741 tcggacaaaa tcgtaaacga aatatcctgc cccgccttca ccttggtaat atcgccctcg
     7801 gcaatctgca ttttgttcaa catcatatcc agattcgcca attggacaat cgtcggcgta
     7861 gactgcgccg cgttcacagt ctgcccctct tccacgagaa tcgccaccac cgtgccgtcc
     7921 atcgttgcgg taatgcgcgt gtagcccaat tccgactcgg cggtattgat ggaaattttg
     7981 ctctgtctga ttagagcctt cagctcggca acattggctt tggcggcggc aagcgcatcc
     8041 tgcgcgcttt ccaaatcttc tttagaggtc gcatcatcct tccacaacgc cgcctgacgc
     8101 ttatatttct tctccgcact gcccaatgca atctgtgccg acaccagctt cgcctgatac
     8161 gtttccaact tggatttttc cgtattgagc gtattggtct gcgaggtcga attgatttcc
     8221 gcaatcaaat cgcccttttt aacctgttgc ccgagtttga cataaagttt cttaatctgc
     8281 cccgatgcct gcgcgccgac cgataccagg ttggacggcg aaatctcccc tgttgcagaa
     8341 accgtccggc tgatgtcgcc gcgcctgacc gtttccgtaa tataagcagc ctgcggttcg
     8401 ggcttcagat aagaccatcc gccccaaacc gctgccgccg cgaccgccgc aacagccgcc
     8461 catttcatca tttttgccat gttttcaatt ctctgtatgc gtcaatccca taaatcggaa
     8521 gcggattcta ccgcaagtcc ctgccccatc caaggcggca aactgccgac aataaaaata
     8581 accatttgtt ttaaatggct tatacaatct gctttcccga aaatttcaat ccgtttacat
     8641 attccgccat ttccgatacg gcgttataat gtcgccgacc gacaaacaaa tggaaacaca
     8701 ttgctatgaa aaacgttcaa aaaggcttta cgctgctcga gctgatgatt gccgtcgcca
     8761 tcctcggcat cctgacgctc atcacctacc cttcctataa aacctacatc cggcgcgtcc
     8821 gcctgtcgga agtcaggacg accctgctgc acaacgcgca gaccatggaa cgttactacc
     8881 gccaaaaagg gacgtttcaa acctacgacc aaaccaaact gaaacaaaac aaatatttca
     8941 agattacctt aagcaaaagc cccgaccact tcacccttac ggccgacccc gatccgatga
     9001 ccaacgaagg cgaaacctgc gtcgttacgc tcaacgacgg cggtaccatt gccgcttccg
     9061 gtacaaacca atcctgcccc ggcttcgact aatcctccgc cgaagcccgc aaacccgtaa
     9121 agatgccgtc cggacacagc ttcagacggc atctttacgg gtttgcccat ccggtttttt
     9181 caaacttgaa acctgccgcc ccttatccgg caaccgcccc gatgccgcgc ctttccgagc
     9241 tgttataata taacatataa tctatatttt tcaacaacat accacttgac ctgtgagatg
     9301 cggaagcgta tgcttaccct tatatcggac aacattccgt ttacaggttt ttttgccgta
     9361 actaaggagc gcaatcatga aaatgcaggc agttgttgtg aataaaaatg tagcgggcga
     9421 tgtggaagta gtcgaacgcg aggttcgccc gttggaatac ggcgaggcat tggtcgaagt
     9481 cgaatattgc ggcgtgtgcc acaccgacct gcacgttgcg gcaggcgact acggcgaaaa
     9541 accgggccgc gtgctgggac acgagggcat cggtttggtt aaagaagttg ccgacggtgt
     9601 gaaaaatctg aaagtcggcg accgcgtcag catcgcctgg ctgttccaaa gctgcggctc
     9661 gtgcgaatac tgcaacaccg gccgcgaaac cctgtgccgc tctgtattga acgctggcta
     9721 caccgccgac ggcggcatgg cgacccactg tatcgtgagt gccgattacg cggtcaaagt
     9781 cccagaaggt ttggatcctg cgcaagcttc gagcattact tgtgccggtg taaccactta
     9841 taaagccatt aaagtttccg gcgttcgtcc gggacagtgg attgccatct acggcgcggg
     9901 cggtttgggc aacttggggg tccaatacgc gaaaaaagta ttcggcgcgc acgttgtcgc
     9961 catcgacatc aacgacgaca agctggcatt tgccaaagaa accggcgcgg atttggttgt
    10021 caacgccgcc aaagaagacg ctgccaaagt gattcaggaa aaaaccggcg gcgcacacgc
    10081 tgcggtcgta accgccgtat ctgctgccgc attcaactct gccgtgaatt gcgtccgcgc
    10141 gggcggacgt gtggttgcca tcggactgcc gccggaatcg atggatttgt ccatcccgcg
    10201 tttggttttg gacggcatcg aagtggtcgg ctctttggtc ggcacgcgca aagatttgga
    10261 agaagccttc caattcggcg cggaaggttt ggttgtgccg aaagtccaac tgcgtgcttt
    10321 ggatgaagca cccgccattt tccaagaaat gcgcgaaggc aaaatcaccg gccgtatggt
    10381 gatcgatatg aaaaaagaat gcggctgcgg tcatcaccac tgatttgacg tggcgataca
    10441 catcgaaatg ccgtctgaac gctgttcaga cggcattttt tatggatttg gatttgattt
    10501 taatctgttc tgtttgaaat accgtctgaa aacccgtatc gcaacacgtc attatagtgg
    10561 attaacaaaa atcaggacaa ggcggcgagc cgcagacagt acaaatagta cggaaccgat
    10621 tcacttggtg cttcagcacc ttagagaatc gttctctttg agctaaggcg agccaacgct
    10681 gtaccgattt aaatttaatt cactataccg tctgaaaacc cgtatcgcaa cacgtcatta
    10741 agaacggcaa gattcaaccg ttctgcaacc cgtttagacg gattccgcca tttccaacgc
    10801 ctgtttgatg tccacggcga cgacgcgcga tacgcctttt tcctgcatgg ttacgcctac
    10861 gagctgctct gccatttcca tggtcaggcg gttgtgggag atgtagagga actgggtttg
    10921 cgccgacatt tctttgacca ggttgcagaa acgcgaggtg ttggcgtcgt ccagcggggc
    10981 atcgacttcg tccaaaaggc agaagggggc ggggttgagg ctgaacaggg cgaacacgag
    11041 gctcatggcg gtgagggctt tttcgccgcc ggagaggagg tggatggtgc tgtttttctt
    11101 gccgggcgga cgcgccatga tggatacgcc ggcggtcagg aggtcgtcgc ctatcatttt
    11161 gagggtggct tcgccgccgc cgaacagggt tgggaagaag gtttggactt tgccgttgac
    11221 ggcatcgaag gtttctttga aacgcgcttt ggttttgtcg tcgatttggg cgatggcttc
    11281 ttccaaaagg gtaatggccg cctgcacgtc ttcgctctgg ctgcggtagt agccgtcgcg
    11341 ttcgcgcact tcttcgagtt cttgcagggc ggcgaggttg acggcgccga gggcttcgat
    11401 ttgctgggtg aggctgccga tactgctgtt caatactttc ggcgattctt tcgccaaggc
    11461 ttcgagcgca tccaaatcgg cggcgcgttc ggtcaggttt tgatggtagc gtttggcgtt
    11521 aatcagggct tcctgctgct gcaacagggc ggtttgggtg gctgcctgaa gctgtggcaa
    11581 ctgggtttgc agggtttgta cgcgggcgta ttgttccctg ccctgcgcct gaatctgcgc
    11641 gagtttgtcc tgcacggcga tgtactcttc atccaaagtt tgtacggctt cgcttaattc
    11701 ttcaagcttg atgtgctgct cgtcgttttg gaactcggtt tcataggcga ggacaagctc
    11761 ttgctggcgt tcctgccagt ctagggtttg ctgttcgagt cgggcaattt gctgccggta
    11821 attttgtttt tgctggttga gcttatggac ggcgacttcg gccagcccgt attggcggtt
    11881 ggcttccaac agggcaagct gcgcctgttt cagacggcct tgctgctctt ggcggctgtg
    11941 cgcggtggtt tgctgttggt gttcgagttc ggcggcggct tcctgcaaag taaagatgtc
    12001 gtctgaaagc ccgtcggacg tgtgttgcaa cacggtctgc tcttccgcca actgcgccag
    12061 ttcgcgctcg atgtgttcgc ggcggatttg cccttggttg gtacgcgcca gaagttcggc
    12121 ggcgcgttgc tgcgcctgac tgtactggcg cgtgtgctgc tgttgctgct gcatcaggtt
    12181 tttatgttgc acttcggacg agcgtacagt tgcctccgcc tgtttgaacg cggcttcggc
    12241 ggtggagagt tcgggggcga ggttgtccag ttcggacgcg atgccgtcga gacgggcttt
    12301 ttgggcaatc agactttcct gcgcgggttt ggcatagagc aggacgctga ctttatcgac
    12361 ctgatggcct tcgggcgtga gccagatttg gtgtgcgccc aaatcgcttt gatgcgcgag
    12421 ggcgtaactc aaatcaggcg cacacaatac gccgtcgagc cagtggtgca atgccgtctg
    12481 aaacggcggc tgcgcttgga tttggttcag caatgcctgt acgggcaggg attttttgat
    12541 gccgccggag agaccgtctg aaagccacgc cgcctgcccc tgcggcaaag gcgcgggggg
    12601 aacgaaacca tgcggtacgc tacgggcttg caggcgttcg gcaaggatga cggacaaggc
    12661 gtgctgccac tcggcgggcg cggtgatgtg ttgccacagt tgcggcgcgg cggcgtggtc
    12721 ggttgcctgc cagaaatcgg cggcttcctg ctgttgcgac aggatttgcg acaacgcctg
    12781 ctgctgcgcc tgcaaggtga tgtgttgctg cttcaggctt tggaagcggt ctgaggccgt
    12841 ctgaaacgcc tcgcgggcag cgtgtaaggc ttcttcggcg gcaatgattt gttcttcgta
    12901 atgctcttgc cggctttgca acaaggcggc ggcttcctgc gcggcggcgg tttcggcttc
    12961 gtcgggcagg ttcagggctt ggttttcctg tttcagacga cctttgcgct cttcgtgctt
    13021 ggcaacggtt tgttcggcat gggcaagctg ctgctgcttc aacgccagtt cgcggcggat
    13081 gcggtttgcc tcgtcctgct gggtttggaa ggcggcgttg agcgtagctt gggcttcttc
    13141 caattcgggc agacgctctt cgtgttcggc aacctgcatc gcccattccg ccagctcggt
    13201 ttgcttttct tcgacgtata actcgttttc ttccagctgc acgcggattt gctgctgctc
    13261 ctgccggatg cgttgcatct gcgcctgcgc cgcctgcttg tcgcgttcga tgcgttggtg
    13321 caggttttgc tgatggcgga tttgttcttc caaacgggca atctgctcgc gcaacacacc
    13381 gcgtttgttg cccagttcgt gtaccgcctg ctgctgcgac tgttcggcgg tctgcaaggc
    13441 gtgtacttcg tcgtttaacg cctgaacctg cgcggcggtt tcgtcctgct gcgcctgcaa
    13501 agactgatgc tgcgcggtcg ccttgtcggc ggcggcaagc gattgctgcc attgggcgta
    13561 atcgagcaaa tcctgttgct ggttcagctg cgcggtcagg gatttgtagc gttcggcggt
    13621 ttccgcctgt ttctccagct tttccacctg acgcgctaac tcgttctgca aatcgcccaa
    13681 acgctgcaaa tgctcgcgcg tgtctttcag acggccttcc gtctccttac ggcgttcctt
    13741 atatttggac acgcccgccg cttcctcgat ataggcgcgc aattcctccg gccgcgcttc
    13801 gatgatgcgc gaaatcatcc cctgctcgat aacggcataa ccgcgcgcgc ccacgcccgt
    13861 acccaaaaac aaatcggtaa tgtcgcggcg gcgcacggtc tgattgttga tgaaataagt
    13921 cgattcgccc tgacgcgtca gctgccgctt gatgctcacc tcggcatact gcccccacgc
    13981 cccctgcaaa ctgtggtcgc tgttgtcaaa taccagctcc accgaagccc tcggcgcagg
    14041 acggcgcgtc gccgcaccgt taaaaatcac gtcctgcata ctctcgccgc gaagctgctt
    14101 cgccgaaacc tcacccaaca cccagcgcac cgcgtcaatc acattcgact tgccgcaacc
    14161 gttcggcccg ataaccgcga caagctgccc cggaacatga atcgtggtcg ggtcggtaaa
    14221 agatttgaag ccggagagtt tgatgtgggt caggcgcata atggtcggaa aaaataaaaa
    14281 agaagcgtat tttaacggaa atcccgccgc accaccccat atcttgccgg caaaacctta
    14341 ccgcatcccg cacccgcgat gccgtctgaa gccttcagac ggcattttta acgcgcccga
    14401 accccggttc cgccaatgcc cgatgcttgg cgcggcattt gccgtattgc cccaccgccg
    14461 ggcgcgttat aatccgcccc gacatccgcc cgattggaaa cctgcctatg aaacccgcct
    14521 atttcatttc cgacctgcat ttgagcgaaa agcagcccga actgaccgcg ctgttgctgc
    14581 gttttttacg ttcttccgcc gccaggcagg cgcgggcggt ttacattttg ggcgatttgt
    14641 ttgatttttg ggtgggcgat gacgaagttt ccgagttgaa tacttcggtt gcgcgtgaaa
    14701 tcaggaaatt gtccgacaaa ggcgttgccg tgttcttcgt caggggcaac cgcgacttcc
    14761 tgatcggtca gaatttttgc cggcaggcgg gcatgacgct gctgccggat tactcggttt
    14821 tggacttgtt cggctgcaaa accctcatct gccacggcga cactctgtgt acagatgaca
    14881 gggcatacca acgtttccgc aaaatcgtgc atcggaagcg gctgcaaaaa ctgttcctaa
    14941 tgctgcccct gaagtggcgc acgcgccttg ccaccaaaat caggcgtgtc agcaaaatgg
    15001 aaaaacaggt caagcccgcc gatattatgg atgtcaatgc cgcctttacc gcgcggcagg
    15061 tacgcgcctt cggtgcggaa aggctgatac acggacacac ccaccgcgag catatccatc
    15121 acgaaaacgg ctttacccgc atcgttttgg gcgactggca taacgactat gcttcaatcc
    15181 tccgcgtgga cggggacggc gcggtattcg tgccgctgga aaaatgctga aaatgccgtc
    15241 tgaagccttt cgggcggctt ttttttacac gcccctttcc cacactccct ccgccgcatc
    15301 atcaccatcc ttaacccgca ttaatgggcg ttatcgataa aatgcttgac cgattacata
    15361 agattacgta aagtgtgcaa acgcataaca attggtctta ccaatttatc ttttaaccaa
    15421 aatatcgggt gtacgggatt tgaacgtctt tccaaacctc gacgcgcctt atgatgcaaa
    15481 tgcctaaaaa ggattcgtta tggcactttt cctcagcata ttccccatcg tcctgctgat
    15541 ttggctgatg gtgaaaaaaa acagtatgcc ctcctacgtc gcgctgccga ttaccgcagt
    15601 gctgatttac gccatcaaac ttttctactt cgacgatgcg ggcatgctgc tcaacgccac
    15661 cgccgcttcc ggcctcgtca aaacgctcac gccgattacc gtgattttcg gcgcgattat
    15721 gttcaaccgt atgatggaaa ccacgggctg catcgatgtc atccgcaaat ggctggcgac
    15781 catcagcccg aaccccgtag cgcaactgat gattatcggc tgggcttttg cctttatgat
    15841 tgaaggcgca tccggcttcg gcacgcctgc cgcgattgcc gcgccgattc tgatgagctt
    15901 gggcttcaac ccgttgaaag tggcgatttt cactttggtg atgaactccg tccccgtctc
    15961 tttcggcgcg gtcggtacgc cgacctggtt cggtttcgca ccgctgaacc tgagtgccga
    16021 agacatcctc gccatcggca ggcagaccgg cgtaatgcac ttcttcgcag gtttcgtcat
    16081 ccccgtcatc ggcttgggct tcatcgtacc ttggtctgaa atccgcaaaa acttgggctt
    16141 cgtcgccatt gccgtcttct cctgcaccat tccttatgtc gcattggcga tggtcaacga
    16201 agaattcccg tcgctcgtcg ccggcgcgat cggcctgatg gtgtccgtat tcgccgccaa
    16261 ccaaggctgg ggcttgagca aagaccacgc caaagacccg aatgccgaaa aagtgccgtt
    16321 cgcccaagtc gccaaagcac tcgccccttt gggtatgctg atcggcatgc tggtggttac
    16381 gcgcatcaaa cagctcggca tcaaaggcat tttgaccagc aaagaagaat ggttcagctt
    16441 ccaactgccg tttgatttgt ccaaaatcac cgtcagcgac tccctgacga ttactttcgg
    16501 caatattttc ggacaagatg tcagcgcgtc ttaccaaacg ctgtacgtcc cggcttggat
    16561 tccgtttgtg ctgaccgttt ggatttgcat cctgctgtat aaaaccaaat tcaaagatgc
    16621 ctggacgatt tatgccgtaa ctttcaatca aaccaaaaaa ccgctgcttg ccctgatggg
    16681 cgcgctgatt atggttcagc tgatgctggt cggcggcgac aattcgatgg tgaaaatcat
    16741 cggtaaggaa tttgccgcaa tggcgggcga acactgggtt tatttctcgc cgtatctggg
    16801 cgcgattggc gcgttcttct ccggttccaa caccgtgtcc aacctgacct tcggcccgat
    16861 tcagcagcaa atcgccttgg ataccggcct gtccgttacc ctgattctgg cgttgcagtc
    16921 cgtcggcggc gcgatgggca atatggtgtg cctcaacaac atcatcgccg tatgtaccgt
    16981 attggatgtg aaaaattccg aaggtgcgat tatcaagaaa accgttatcc cgatggcgat
    17041 ttacggcgtg attgccgtcg tcgcggcaat gattttcttc ctctaggcga aacgccacca
    17101 tccatagcgg cagcacgcgg ggaacggaaa ccgccgttcc ccgtctgggc ggcgcgtcgg
    17161 gcagaatccc ggtgccggaa caagcccgcc ccgccaaaca aatgccgtct gaaaccggaa
    17221 aaggcttcag acggcatttt tccgccgctt gcgttcaggc gtaatgatga tgcggcatct
    17281 tgccgtcaag gcggtagcgc gtgccgcaat acgggcaggc aacgctgccc gattctcctt
    17341 cgcacaaagg caaaaacact ctcggatgcc cgttccactg ctcgttgtcg ggtcccgagc
    17401 aatacagcgg cagattttcc ggcaacacgg aaatttcctg cagattcaga ttgtccattt
    17461 gattttcctt tgcgtggttg gcgtgcgccc attttacgcc atcggcaggc taaaggatat
    17521 tttcggcgca aagccgcaat ccgctataat ccccactttt cagacggcat accatgactg
    17581 cgcttaccct tccggaagac atccgccaac aagaaccatc cgttctgctc tataccctcg
    17641 tttccgccta cctcgaacac accgcccaaa ccggcgacga atccctctcc tgcctgtccg
    17701 acgaccagca cacgctgacc gcattctgct acctcgacag ccaagtcgaa gaaggcggct
    17761 tcgtgcaact catcgcctcc ggttacggcg aatatatttt ccgcaacccg cttgccgaca
    17821 gcctgcgccg ctggaaaatc aaagccgtgc cgaaagtctt ggacaaagcc aaagccctct
    17881 acgaacaaca cggcgaaacc atcgaaacgc tcgccgacgg aggcgcagac atcgaatccc
    17941 tgcgcaaaca gttccccgac tttgaagaat gggacggcgc atactacgaa gccgccgaac
    18001 aagacctgcc cctgcttgca gaacacatcc tgtcaaactg ggaaaccttc gcccatatcg
    18061 ggcaggcgtg attgcgtctg tttccagccc gtgtaaaaca gcgtaaaatc ggcaaacccg
    18121 aatgaccttc cgtccccatc aaggagcaag ctatgttctt caagcacatc gaagccgccc
    18181 tcgccgatcc gattctcggt ttgggcgaag cattcaaagc cgaaacccgc cccgaaaaag
    18241 tcaacctcgg catcggcgtt tataaagacg catccggcgc gacacccatt gtcaaagccg
    18301 tcaaagaagc cgaaaaacac ctgttggaaa gtgaaaccac caaaaactac ctcaccatcg
    18361 acggcgttgc cgattacaac gcgcaaaccc aaatcctgct gttcggcaaa gaccacgaaa
    18421 tcatcgccag ccgccgcgcc aaaacagcgc aaagcctcgg cggtacgggc gcattgcgta
    18481 ttgcggccga gtttgccaaa cgccagttga acgcgcaaac catctggatt tccaatccga
    18541 cctggccaaa ccacaacgcc atcgccaagg cggtcggtat ccaagacaaa ccttatcgtt
    18601 attatgatgc cgccaaacac ggtttggatt gggacggcat gattgaggac ttgagccaag
    18661 cgcaaaaagg cgacatcgtc ctgctgcacg gctgctgcca caatcctacc ggcatcgacc
    18721 ctacgcccga acaatgggaa actttggcaa aactttctgc cgaaaaaggc tggttgccgc
    18781 tgtttgactt tgcctaccaa ggcttcggca atggtttgga agaagatgcc tacggcttgc
    18841 gcgtgttctt gaaacacaat acagaattgc tgattgccag ctcttattcc aaaaacttcg
    18901 gtatgtacaa cgagcgcgtc ggcgcgttca ctttggtggc cgaagatgaa gaaacagcag
    18961 cccgcgccca cagccaagtc aaaaccatca tccgtacctt gtattccaac ccggcttcac
    19021 acggtgcgaa caccattgcg ctggtgttga aaaatgatga tttgaaagca caatggattg
    19081 ccgaactcga tgaaatgcgc ggccgcatca aagccatgcg ccaaaaattt gtcgggttgc
    19141 tcaaagccaa aggtgcaagc caaaactttg atttcattat caaacaaaac ggtatgttct
    19201 ctttcagcgg cttgactccc gagcaagtcg accgcctgaa aaacgagttt gccatttatg
    19261 ccgtccgctc cggccgcatc aacgtcgccg gcattaccga cgacaacatc gattatctgt
    19321 gcgaaagcat cgtgaaagtc ttgtaaaaca aaagcgcata cgaaatgccg tctgaacctt
    19381 aaccagttca gacggcatct gttttttgtt aaaaaacaat aaaccataac aatttcaaac
    19441 aagctaaaaa acatctataa aatcaaccca atatgatgaa ctcaaataaa aaatacgaat
    19501 atcatcattc tcaaattcaa aatttgaacg ccacagtatt atccgaagcc acaaaaacct
    19561 ttccgccgtc attcccgcgc aggcgggaat ccgggaaccc aatgctgcaa gaattgatta
    19621 gaaacaactg aaaccgagca aactggattc ccgcctgcgc gggaatgacg aaataaggcg
    19681 agtaaaactg atgaaacaaa ttgattaaaa acaataactt atattatttt atttaaattt
    19741 tcgataaaat caattttccg tattcaatac tgctccaatc aattcccgcg caccgtcgtc
    19801 cgccaatttc ttagcgacag cgcgtccgag cgcgtcggca tattcggcag gggcttgcgc
    19861 gtccgcctgc aacacaaccg aaccgtcggg gtgtccgacc agtccgcgca aggtcagcaa
    19921 gccgttttct tccgtgcaat atgcggccag cggcacttgg cagcttccgc ccaaagcgcg
    19981 cgccaaagct cgttcggccg taacgcaggc gtgtgtggta tcgtggttta gcggttttaa
    20041 aacctcatac aaatcttcgc ggtgcgcggc aatttcgata cccaatgcgc cttgtccggc
    20101 ggcaggcagg ctgtcggatt ccgacaaaat catgcggatg cgtccgtcca atttcagacg
    20161 ctgcaaaccg gcggcggcca aaataattgc gtcgtattcg ccgttatcga gtttggacaa
    20221 acgggtttgc acattgccgc gcaaaggttt gataagcaaa tgcggatagc gcgcgcgcaa
    20281 ctgggcttcg cggcgcaggc tggatgtacc gacgacagcg cctttgggca tttcttccaa
    20341 acgcgcgtat tggttggaca caaacgcgtc aaacggattg gcgcgttcgc cgatggcggc
    20401 aagcgcgaaa ccttcaggca aatccatcgg cacgtcctta atcgaatgca ccgccaaatc
    20461 ggcgcgcccg tcatataaag cctgttccaa ctctttgaca aacaagcctt taccgccgac
    20521 ttttgacaaa gttttgtcca aaatctgatc gccgcgcgtg gtcatgccca aaatctcgac
    20581 ttcgcaatcg ggatacagag ccttcagacg gccttggata tgctttgcct gccacatagc
    20641 aagcaggctt tcgcggctgg cgatgacgag ttttttcggg ttcatgttca atgcgttcaa
    20701 agaatggaaa cggcgtgaag tctatcatat tttgaacgtt tcttcagacg gcaccgtcag
    20761 gtctatgtta tatttacacc cacaaaacac gaaacccgcc acaatgaaac gcaccaaaaa
    20821 ataccctttc ttaacgttgc agcggcaacg tttccatttg aactttgaaa acgcctcttc
    20881 cgccgccggc atcccagccg aacgcgattt ctaccgctgg gcgtggtctg ccttgaaaaa
    20941 tgaataccgc cgcgccgaca tcagcctgat tcttctggac gaagaagaag cccgagccta
    21001 caaccgcgac taccgcggca aagattacgc caccaatgta ttgagtttcg cgctcaacga
    21061 aggcgaaatc ctgccctgcc aagtttcgga aaaactgtac ggcgatttga ttatctgccc
    21121 gcaagtggtt ttgaaagaag ccgccgaaca gggcaaaacg cccgaacggc attttgccca
    21181 cctgaccata cacggcgttt tgcacctgat gggctacgac cacatcgaag acgatgaagc
    21241 cgaaataatg gaagccgaag aaatccgcct gatgctggcg gcaggcttcc ccaaccccta
    21301 ccaagaggac gaatattaaa atggacggta cgcagtcgaa acccaagttt ttcgaacgcc
    21361 tgatctcccg acttgccggc gagcccgact ccgccgaaga cgtattgacc ctgctgcgcc
    21421 aagcgcacga gcaggaagtt tttgatgcgg atacgctttt aaggttggaa aaagtcctcg
    21481 atttttccga tttggaagtg cgcgatgcca tgattacgcg cagccgtatg aacgttttaa
    21541 aagaaaacga cagcatcgag cgcatcaccg cctacgtcat cgataccgcc cattcgcgct
    21601 tccccgtcat cggcgaagac aaagacgaag ttttgggcat tttgcacgcc aaagacctgc
    21661 tcaaatatat gttcaacccc gagcagttcc acctgaaatc cgtcttgcgc cctgccgttt
    21721 tcgttcccga aggcaaatct ttgaccgccc ttttaaaaga gttccgcgaa cagcgcaacc
    21781 acatggcgat tgttatcgac gaatacggcg gcacgtcggg tttggtcacc tttgaagaca
    21841 tcatcgagca aatcgtcggc gaaatcgaag acgagtttga cgaagacgat agcgccgaca
    21901 atatccacgc cgtttcttcc gaacgctggc gcatccatgc agctaccgaa atcgaagaca
    21961 tcaacacctt cttcggcacg gaatacagca gcgaagaagc cgacaccatt ggcggcctgg
    22021 tcattcaaga gttgggacat ctgcccgtgc gcggcgaaaa agtccttatc ggcggtttgc
    22081 agttcaccgt cgcacgcgcc gacaaccgcc gcctgcatac gctgatggcg acccgcgtga
    22141 agtaagcacc gccgtttctg cacagtttag gatgacggta cgggcgtttt ctgtttcaat
    22201 ccgccccatc cgccaaacat aaaaatgccg tctgaaaccg ttgcaagttt cagacggcat
    22261 ttcgcttatc cgcttatttt ttgctcaaag tcagccccgt ccaaccgaat aacagggtca
    22321 aagccaaact caaatagcag aaaaaggcat acggcagata ttcccaaacc ggcacgccca
    22381 gcgcgtggct gatgaacacg ccgcatacgc tccacggtac gagcgggtta atcaccgtcc
    22441 ccgcatcttc cagcgtccgc gacagattgc gggaatgtag tccgagcctg tcgtaaacgg
    22501 gtttaaacgt ttctccagaa agcaggatgc tcaaatattg ttcgccaatc aggaaattaa
    22561 ccccgaccga agtcatggca acgctgaacg tcgcgcgtcc ggcattcgtc aagaaggtac
    22621 ggatggcctc caacagggaa ggaatcacac cgagcgcaaa caacagtccg cccaaactca
    22681 tcccgagaat cacgatggtt tgcgtgaaaa acatactttc caaaccgccg cgcgaaatca
    22741 gtttgacaac atctttaaac gcttcgcctt cgagtttgta gccgccgtaa aaccacgcac
    22801 cgagctgacg cagatcgggc gtgctgtgca gatacgttac agcaacggca accatgacgg
    22861 taaagagcat ggcgacgacg gcgttgatgc gcatcaatgc caaaatgacc aacagcgcaa
    22921 acggaatcag cgaatagccg tgcaccaatc ccgtggcttc aagctggctg cggaaggatt
    22981 cgacgctgtt caaatcctgc gcggcgacat tcggcaaaag ccaaagcatc agtgccgcac
    23041 taatgagcca cgcggggatg gtggtgtaca tcatattttt gatgtgctca aacaagtcga
    23101 tgccgacgat ggacgcggaa atacccgtcg tatccgaaag cggggacatt ttgtcgccaa
    23161 aaaatgcgcc cgaaacaatc gcgcccgccg tcatcgccat atcggcctga aacgccgccg
    23221 ccatccccat aaaggcaacg ccgacagtgg cgcaggtggt caggctgctg ccgatggaca
    23281 cgccgatgac ggaacacagc gcgaaggcgg aaaaataaaa ataagtcgga gaaatcagcc
    23341 cgaaaccgta atacatcagc gtcggaatcg caccgctcat catcagtgcg ctgaccatca
    23401 gcccgatgaa gaaaaacagg taaatcgcgc ccataccctg attcaacgcg cctatcatgc
    23461 cctgctgcat atcgttgtat ttcaaaccgc gcgccaagcc gtacaaaatc agcacgacga
    23521 tggcggcaat aatggacata tgcggcagcc actccaatga aatgatggta tagcccatcg
    23581 cggcaatcag agcgacgacg acggcaagtg cctcaccgcg cggcatatcg agtaaggatt
    23641 tgaaagcaaa catgattctt tgcccgattt gatataaagt taacggattc taacacacca
    23701 taccgaatcc gcccaacgtt ttcagacggc attttagcgt tttacaaaag ccggacagaa
    23761 acaaaacggc aattttctca atttaaatta atttttttga aatataattt tttatttttt
    23821 tgtttgttgg catataagac ttttatggat tgaagtatta aaacctcggc acacacgccg
    23881 caaacaccgg tacgcaaaaa tgccgtctga aagatgttca gacgacattt ctcaagaaaa
    23941 agattatttg tccgccctat atccgtacac cgcaaaaaac acgatataca gataacagat
    24001 gacggaaacg acaaacgaag acatcagggt gtaagtatct gccacccagc cctgcacgac
    24061 aggaacgacc gcaccgccga caatcgcggt acacagtaca ccggaagccg cgttggtaaa
    24121 ttttcccaat cctttggttg ccaaagagaa aatcgtcggg aacataatcg agttgaaaaa
    24181 accgatggca agcagcgacc acatcgccac atccgcattg ccgctacccg tcgccatcgc
    24241 gacggcaagc agtacgaccg cagccgatgc gttaaacgcc aaataacggt tgggcgcgaa
    24301 tttcgccatc accgccgaac cgaggaaacg tccgaccatc gcgccgcccc aatagaacga
    24361 caggtaatgc gcggcagaag catgatccag ccctttcaga taacccaata cgttgaccat
    24421 caacgaaccg atagacacct ccgcgccgac atagcagaaa atgcctgccg taccgaacac
    24481 gagatgtttg tattgccata cgctggtttt gccgtcgtgg ttgtgcgcgc tttcctcggc
    24541 ggcaattttg cgcgcgtcgg gcagccggat cattttcacg aaaacggcaa ggataatcag
    24601 cagccccgcc agtcccaaat agggaatctg tacggaagaa atctgttccg ccttgctgac
    24661 ggtttgggtt gcgtccgcca gaatcaggaa cgcgccgatt tgcggcgcaa tggtcgtacc
    24721 caaagcgtta aacgcctgaa ccagcgtcag tgttgccgat tccttgccgg gtttcgccag
    24781 cagggtaaca taaggattac cggcgacctg aagcagcgtt acgccggaag ccaaaataaa
    24841 caacgcgccc aaaaataccg cgtaagaatg gctacccgca gcaggataaa acagcaggca
    24901 tccgaccgcc gtcagcagaa agccgccgat aacgccgttt ttgtagccga ctttgccgac
    24961 aaaagccccc atcgggatgg acatcaccgc ataggcggta aagaaacaga attggatcag
    25021 catcgcctga acgtaagaca ggtcgaaaat ttctttcaaa tgagggataa ggatgtcgtt
    25081 catgcaggta ataaaaccca tcatgaagaa cagcgtggtc aagacgacca atggggacgt
    25141 atgattgttt tgtgattgcg cagacatgat ttgcctttcg tggtgtcgga atattacccg
    25201 tttcaagaaa atagaaattg acatttctta acggcaatgc aaatcatact gatatttgaa
    25261 cattttgcaa actttatcgg atgcaggcaa tgcgccgatc tgaaattcaa gcagcagaaa
    25321 ttaatatttt acagttgctt gcatagattt taatagattt taaaaaattc cggcggactt
    25381 gcaaaaaccg ttcccaataa aatgaaaatc cgccgccgta tccggaaaat tcggaaatgc
    25441 cgcatcgtgg catcaaaacc ggagcggaaa gaatgtcgta tgcccgacaa aaaaggggca
    25501 tacggctgtg ccgtattttt tgcaccgtcc tccgcccttc ggcgggggcg tgaaaatata
    25561 gtggattaac aaaaatcagg acaaggcgac ggagccgcag acagtacaaa tagtacggaa
    25621 ccgattcact cggtgcttca gcaccttaga gaatcgttct ctttgagcta aggcgaggca
    25681 acgccgtact ggtttttgtt aatccactat accgtacaat ccgtcgccga tttttgcctg
    25741 tttgagactg tttgcaatga tgcccgccta cgccgcgctc gccttttccg ccttcacttc
    25801 cgccaccctc ctgcccggca catccgaagc cgcctttgcc ctgttcgtcc accgctttcc
    25861 cgaacacgca tacggcgcgt tgctgtgcgc cggccttgcc aacggattgg gcagtatggt
    25921 ttcctattgg atggggcgtt tgctgccctc ccgaaaaatg ccgtctgaaa aaacactgaa
    25981 tctgatgcgg cgtttcggta tttggctgct tgcgtttgcc tggctgcccg tcgtcggcga
    26041 cgcactgccg cttgccgccg gctggctgcg gttgaatccg tggacaagcg ggctgatgct
    26101 ggttatcggc aaaacggcgc gttacgcctt cattctgtgg ggaatgcaat attacgccgc
    26161 ctgaaccgtg cgttataatg ccgaccgcac aaaccgactg cacaaaaaaa tgaacagaca
    26221 catccgccaa gaaattttcg aacgcttccg cgccgccaac ccccatccga ccaccgagct
    26281 gaatttcaac tcccctttcg agcttttaat cgccgttctg ctttcggcgc aggcgaccga
    26341 tgtcggcgta aacaaggcga cggcgaagct gtttccggtt gccgatacgc cgcaggcaat
    26401 gctggatttg ggcttggacg gcgtgatgga atacacgaaa accatcgggt tgtataaaac
    26461 caagtccaag cacatcatgc agacctgccg catcctgctg gaaaaataca agggcgaagt
    26521 gccggaagac cgcgaggctt tggaatcgtt gccgggcgtg gggcgcaaaa cggcaaacgt
    26581 ggtattgaac acagcgttcg gacaccccgt tatggcggtc gatacgcata ttttccgcgt
    26641 gtccaaccga accaaaatcg cacccgggaa agatgtgcgc gaagtcgaag acaaactgat
    26701 gcgcttcatt cctaaagaat ttctgatgga cgcgcaccac tggctgattt tgcacggacg
    26761 ctacacctgc aaggcactca aaccgcaatg ccaaacctgc atcatcaacg atttgtgcga
    26821 atatcccgcc aaagcctgac tgccgccgcc cttcaaaatg aaacgcttga aaattcggac
    26881 ggacgttgta gaatttcaac ctatgccgtc tgaagccgac aaaatgcgct tcagacggca
    26941 ttaaagccgg taccggcatc cgacaaagga caaaacgtgt tcaaaaaata ccaatacctc
    27001 gctttggcag cactgtgtgc cgcctcgctg gcaggctgcg acaaagccgg cagctttttc
    27061 ggtgcggaca aaaaagaagc atccttcgta gaacgcatca aacacaccaa agacgacggc
    27121 agcgtcagta tgctgctgcc cgactttgtc caactggttc aaagcgaagg cccggcagtc
    27181 gtcaatattc aggcagcccc cgccccgcgc acccaaaacg gcagcagcaa tgccgaaacc
    27241 gattccgacc cgcttgccga cagcgacccg ttctacgaat ttttcaaacg cctcgtcccg
    27301 aacatgcccg aaatccccca agaagaagca gatgacggcg gattgaactt cggttcgggc
    27361 ttcatcatca gcaaagacgg ctatattctg accaatacgc acgtcgttac cggcatgggc
    27421 agtatcaaag tcctgctcaa cgacaagcgc gaatataccg ccaaactcat cggttcggat
    27481 gtccaatccg atgtcgccct tctgaaaatc gacgcaacgg aagagctgcc cgtcgtcaaa
    27541 atcggcaatc ccaaagattt gaaaccgggc gaatgggtcg ccgccatcgg cgcgcccttc
    27601 ggcttcgaca acagcgtgac cgccggcatc gtgtccgcca aaggcagaag cctgcccaac
    27661 gaaagctaca cacccttcat ccaaaccgac gttgccatca atccgggcaa ctccggcggt
    27721 ccgctgttca acctgaaagg acaggtcgtc ggcatcaact cgcaaatata cagccgcagc
    27781 ggcggattta tgggcatctc ctttgccatc cccatcgacg ttgccatgaa tgttgccgaa
    27841 cagctgaaaa acaccggcaa agtccaacgc gggcaactgg gcgtgattat tcaagaagta
    27901 tcctacggtt tggcacaatc gttcggtttg gacaaagccg gcggcgcact gattgccaaa
    27961 atcctgcccg gcagccccgc agaacgtgcc ggcctgcagg cgggcgacat cgtcctcagc
    28021 ctcgacggcg gagaaatacg ttcttccggc gaccttcccg ttatggtcgg cgccattacg
    28081 ccgggaaaag aagtcagcct cggcgtatgg cgcaaaggcg aagaaatcac aatcaaagtc
    28141 aagctgggca acgccgccga gcatatcggc gcatcatcca aaacagatga agccccctac
    28201 accgaacagc aatccggtac gttctcggtc gaatccgcag gcattaccct tcagacacat
    28261 accgacagca gcggcggaca cctcgtcgtc gtacgggttt ccgacgcggc agaacgcgca
    28321 ggcttgaggc gcggcgacga aattcttgcc gtcgggcaag tccccgtcaa tgacgaagcc
    28381 ggtttccgca aagctatgga caaggcaggc aaaaacgtcc ccctgctgat catgcgccgt
    28441 ggcaacacgc tgtttatcgc attaaacctg caataacaca cataatttaa ccaaaatatt
    28501 ttttaaatga taaaatgccc gtcaataccg attaacgggc attttacaaa aatcagttgg
    28561 ttgattttaa acaaatattt tttacgacaa aaaatatttc cgcccatttt gccaacaaaa
    28621 cccaaaccgg aggcggacag atacgctccc atccggacgc ttaacacccc cataaccatg
    28681 cagctgatcg actattcaca ttcatttttc tcggttgtgc cacccttttt ggcactggca
    28741 cttgccgtca ttacccgccg cgtactgctg tctttaggca tcggtattct ggtcggcgtt
    28801 gcctttttgg tcggcggcaa ccccgtcgac ggtctgacac acctgaaaga catggtcgtc
    28861 ggcttggctt ggtcagacgg cgattggtcg ctgggcaaac caaaaatctt ggttttcctg
    28921 atacttttgg gtatttttac ttccctgctg acctactccg gcagcaatca ggcgtttgcc
    28981 gactgggcaa aacggcacat taaaaaccgg cgcggcgcga aaatgctgac cgcctgcctc
    29041 gtgttcgtaa cctttatcga cgactatttc cacagtctcg ccgtcggtgc gattgcccgc
    29101 cccgttaccg acaagtttaa agtttcccgc gccaaactcg cctacatcct cgactccact
    29161 gccgcgccta tgtgcgtgct gatgcccgtt tcaagctggg gcgcgtcgat tatcgccacg
    29221 cttgccggac tgctcgttac ctacaaaatc accgaataca cgccgatggg gacgtttgtc
    29281 gccatgagcc tgatgaacta ttacgcactg tttgccctga ttatggtgtt cgtcgtcgca
    29341 tggttctcct tcgacatcgg ctcgatggca cgtttcgaac aagccgcgtt gaacgaagcc
    29401 cacgatgaaa ctgccgtttc agacggcagc tggggcaggg tttacgcatt gattattccc
    29461 gttttggcct taatcgcctc aacggtttcc gccatgatct acaccggtgc acaggcaagc
    29521 gaaaccttca gcattttggg ggcatttgaa aataccgacg tgaacacttc gctggtattc
    29581 ggcggcactt gcggcgtcct tgccgtcgtt ctctgcacgc tcggcacgat taaaaccgcc
    29641 gactatccca aagccgtttg gcagggtgcg aaatccatgt tcggcgcaat cgccatttta
    29701 atcctcgctt ggctcatcag tatggttgtc ggcgaaatgc acaccggcga ttacctctcc
    29761 acactggttg cgggcaacat ccatcccggc ttcctgcccg tcatcctctt cctgctcgcc
    29821 agcgtgatgg cgtttgccac aggcacaagc tgggggacgt tcggcattat gctgccgatt
    29881 gccgccgcca tggcggtcaa agtcgaaccc gcgctgatta tcccgtgtat gtccgcagta
    29941 atggcggggg cggtatgcgg cgaccactgt tcgcccatct ccgacacgac catcctgtcg
    30001 tccacaggcg cgcgctgcaa ccacatcgac cacgttacct cgcaactgcc ttacgcctta
    30061 accgttgccg ccgccgccgc atcgggctac ctcgcattgg gtctgacaaa atccgcgctg
    30121 ttgggctttg gcacgacagg cattgtattg gcggtgctga tttttctgtt gaaagataaa
    30181 aaacgcgcca acgcctgacc cctttccctg ttcagacggc atatgccgtc tgaagctttt
    30241 tggagcaaac cgcaatgacc gtcccccata ttccgcgcgg ccccgtcatg gcagacattg
    30301 ccgccttccg cctgaccgaa gaggaaaagc aacgcctgct cgatcccgcc gtaggcggca
    30361 tcatcctctt ccgccgcaac ttccaaaaca tcgaacaact caaaacactc accgccgaaa
    30421 tcaaagccct gcgcacaccc gaactcatta tcgccgtcga tcacgaaggc ggcagggtgc
    30481 aacgtttcat cgaaggcttc acccgcctgc ccgcaatgaa cgttttaggg caaatttggg
    30541 acaaagacgg cgcgtccgcc gccgaaaccg ccgccggaca agtcggccgg gtgttggcaa
    30601 ccgagctttc cgcctgcggc atagacctct ccttcacccc cgttctcgac ttggactggg
    30661 gacaatgcgc cgtcatcggc aaccgcagtt tccaccacaa tcccgaagcg gtcgcccacc
    30721 tcgccctcgc ccttcaaaaa ggtttggcaa aaggcggtat gaaatcctgc ggcaaacatt
    30781 ttcccggaca cggatttgtc gaaggagaca gccatctggt cttgccggaa gacaggcgca
    30841 gcctgtccga actcgaagcc gccgacctcg cccccttcca cattatgagc cgcgaaggta
    30901 tggcggcggt tatgcccgcc cacgtcgttt atccacaagt ggacacaaag cccgcagggt
    30961 tttccgaaat ctggctcaaa caaattttgc gccgcgatat cggattcaaa ggcgtgattt
    31021 tctcggacga tttgactatg gaaggcgcgt gcggggcagg cggcatcaaa gaacgcgccc
    31081 gcatttcctt tgaggcaggt tgcgacatcg tcctcgtctg caaccgtccc gaccttgtgg
    31141 atgaactgcg cgaagatttc cgcattcctg acaatcccgc tttggcgcaa cgttggcaat
    31201 atatggccaa cacgctcggc agtgccgccg cgcaagccgt tatacagacg gcggatttcc
    31261 aagcggcgca agcctttgtt gccggccttg cctcgccgca agacacggcg ggcggcgtaa
    31321 aagtcggcga agccttttaa atccgcccga cacgcggcaa aaaggcaaaa cggtataggc
    31381 acgccgtatt ttttgcgcta taatcacgcc ttacttaatt cggaatgtag cgcagcccgg
    31441 tagcgcactt cgttcgggac gaaggggtcg gaggttcgaa tcctctcatt ccgaccaaat
    31501 ccaaaccgcc tcggtatcca ttgccgaggc agtttttcct tttgacgtca acagaaaaat
    31561 gccgtctgaa acagaatgag acctttgcaa taacataggt tactaaaatt ttatgctcaa
    31621 tctcattttc aaaatgcaaa acttttctga tttttcctac tttttgctca atattaggaa
    31681 agttttaggc aattgaaaat tttttggcac atttttatgc gtcaaatttc gttaacagac
    31741 tatttttgca aaggtctcag aatgctgttt cagacggcat ttcgttttcc ctaaccctgc
    31801 catttgcgcg cactggcaca gagtatcggc aaggttcaaa agaccatcgg aatttaggaa
    31861 gccgggcttg tacccaacgg cggcatctta ttcagtaagg ctccgcctca atcaggcagc
    31921 ggaaatgtgg gcagtttcac gcggaagcgg cacacttttc ccatatgtca aacatcggta
    31981 aaaccgtacg ccgtcccttt gagttacact gcttaaacta ctctattccc gcccatttgt
    32041 gcgtctgcac gctcaactgc caatgcaccg gcgcgtcggg gcgactgttt aaaataccga
    32101 tttggcggat ggtgtcgtag atgttcatcg caccgtcttg ttcgcagggc gaaaggtagt
    32161 aatgatgcgc gcggattttg cgttccatat tttcacagaa cgcaacgaca tcgccgtcgg
    32221 caacaatccg cacttcgtcg gctgtttcga tacagctttt ttcgtatttg gcggcatagc
    32281 aggctttggg gctggtggcg acatagtcga tttgcggcgg cgcgggattg agtccgttgg
    32341 tttcgataca gaggaaataa ccttccgcct tgagcatgtc cagcagcata tcgagatgcg
    32401 gctggatggt cggttcgccg ccggtgatga tgatgttgcg ggcggcatag gctttcagac
    32461 ggcctaagat gtcggacagg ctcatcatgc cgaatgtcaa ataatcggta tcacaccagc
    32521 cgcaggcgag gttgcatttg cccaagcgga cgaaaacggc gggcatgccc gtgttccagc
    32581 cttcgccttg caggctttca aagatttcga cgatgcggta ttggggattt tcgggggcga
    32641 cgctgatttt tttcataccg ccaacaccgc cgccgctgcc aaaaatgcgg gcaaaccttg
    32701 tttgacgagt atgcctttgt tgccggaaga gaacgcgccc cacgcggcgg caatcaggac
    32761 gaagccgaga aacagtaccg ttgcgccgta caccgcgtta tccggagcgg caaaccgcga
    32821 ccacaccagc ccgatgccga gaaagccgtt atacagccct tggttgccga acagggtctg
    32881 cacgcgattt tgttccataa attcataagg cagcttgaac gtttccgccg ctttttcgct
    32941 gggaatctgc gtcatttcaa gccaggcgat gtagaaatgt tcgacggcga cgaagaggac
    33001 tagggggatg aagaggagtt tcatggatat tccttttaat gggtgtattt gatatggaag
    33061 gtcgtctgaa aaaacgggga atccgtacag gagaaaaaac cgttttacgg taaattcaaa
    33121 taaggacgct ctaaaccgtc ctttccgcgc aggcgggaat ccagagattt ggaattgctg
    33181 caatttttaa atattcctga taaatcaagg tctggattcc cgcctgcgcg ggaatgacgc
    33241 ggttgggcat gtcttattgg aatttactat atcgatacgt tcaagatatt ccctactccc
    33301 cttcatactc cgcacacgat gtcggcgttt cccacaattt cacgcggcac acgttcagcc
    33361 ccgcgttttt cagacggtcg tacatttcga ccgccatatt ttcggcagtg gtgcggcagg
    33421 gcaggcgcag ggttttcatg ttccagccct ccaaaagcgc ggcgatttgg cattcgcggc
    33481 tgttgccgcc gtggtagatg aaggcgtggt cgaaggggtc ggtaatgtgt tgtttgacaa
    33541 tggctttcaa gtcggtaaag tccatcacca taccgtcttt cgcgccgcct ttgataatgc
    33601 cgtctgaaac ggtgatttcg agtttgtagg tatgcccgtg caggttttgg catttgccgt
    33661 catgcccgtc gagcatatgc gaggagtcga aggtgaagat tttggtgatt ttcatgggtt
    33721 ctcaaatggg caaatagccc tgaaagccga tttcggcggg tttgccgtgc ggattcgggg
    33781 tcaggcggat atggaaattg ccgatgcggt cgagcgcaag caggttgtcc acgtctttta
    33841 agttgtcttt atgctgctga acatagctgt cggtcaacag ggtttgcagg gctttttgtt
    33901 tggcttcggc aggcgtggcg gcggcgaaca acccgaaagc gtgcgcttcg gcgagctgcc
    33961 ctgcgcgata gccgccgaca ttggcaaaat acaggcgcgg ggcgttttca gacggcattt
    34021 tcgttttggg cgtttcggat acggcgatgt cgtaaccgtc cgcccattcg acaacctgcc
    34081 agccgtcgat gtggattttg tccgcatcgc cgaaccacgc ggctttgagt gcggggacgg
    34141 cttcacggta gtcgtcgcac acggcaaatt ggatgtcgtg cacttcgata ttcgacctgc
    34201 cggcattgcc gccgaggtaa aacatatgga gcttgggcat atcgtattcc ttatggtttg
    34261 cgcgtgttca gacggccttt ttactttcca gatattccgc cagcccgcgt tcgcgcaaga
    34321 tacagctcgg gcattcgcgg cagccgccga cgatgccgtt atagcaggtg tgggtttgct
    34381 cgcggatata gtccagcgcg cccatttcgt ccgccaacgc ccaagtttgc gccttggtca
    34441 gatacatcag cggcgtgtgg atttgaaaat catagtccat cgccagatta agggtaacgt
    34501 tcatggattt gacaaacacg tcacggcagt cgggatagcc ggagaagtcg gtttcgcaca
    34561 cgcccgcgat gatgtgccgt atcccctgcc ctttggcgta aatcgcggca tagagcagga
    34621 aaagcgcgtt gcggccgtct acaaaggtat tcggaacgcc gttttcggca gtttcgatgg
    34681 cggcggtgtc gtccatcagg gcattgtgcg taatctgccg catcaggctc aagtcgagta
    34741 cggtttgttt gacgcccaaa tcctgcgcaa tccagcgggc acgttccagc tcgacggcat
    34801 ggcgttgccc gtattggaaa gtaatggctt ggacgttttc gcgcccgtag gtttggattg
    34861 cctgaatcag gcaggtggtc gaatcctgac cgcccgaaaa gatgaccaag gctttttggt
    34921 ttgacatatc gaatcctagt tttggtgacg cgggaggttt tcgaaccgcg aagttggaca
    34981 gtaaagacgc ggattataca ggcaaacgcg ttcagacggc atctgaacgc catgccgtct
    35041 gaacacgcgg cttatgccga cttgatggcg agttccgcca agcgtttgct ttgtgcgaag
    35101 gcgatattgt tttcttcggc ggtcgggacg gggccgctgt cgggaatatc tttttcgacg
    35161 gcttcgcaga cggcggacac tttggggacg gtgcgcaata cggcttcgca accttcaacg
    35221 ctgtcgatgc cgcgagcgat tcggcgggcg ggattgcggg tgctgccgtt ttggaaatag
    35281 tagaggacga ggattttgag ggggatttgg gttcatgggc ggattccgtt aaaatggttc
    35341 ggtcttcaga cggcataaca gttgttccga tgccgtctga aacagccgca gttcaggcat
    35401 ttgttcctga cggcaaaatt ggctataaca tcgggcatca atgcccaact tactgaccgg
    35461 gcaagaaatt atgacctttt tacaacgttt gcaaggtttg gcagacaata aaatctgtgc
    35521 gtttgcatgg ttcgtcgtcc gccgctttga tgaagaacgc gtaccgcagg cggcggcaag
    35581 catgacgttt acgacactgc tggcactcgt ccccgtgctg accgtgatgg tggcggtcgc
    35641 ttcgattttc cccgtgttcg accgctggtc ggattcgttc gtctccttcg tcaaccaaac
    35701 cattgtgccg cagggcgcgg acatggtgtt cgactatatc aatgcgttcc gcgagcaggc
    35761 gaaccggctg acggcaatcg gcagcgtgat gctggtcgtt acctcgctga tgctgattcg
    35821 gacgatagac aatacgttca accgcatctg gcgggtcaat tcccagcgtc cgtggatgat
    35881 gcagtttctc gtctattggg ctttactgac gttcgggccg ctgtctttgg gcgtgggcat
    35941 ttcctttatg gtcggctcgg tacaggatgc cgcgcttgcc tcaggtgcgc cgcagtggtc
    36001 gggcgcgttg cgaacggcgg cgacgctgac cttcatgacg cttttgctgt gggggctgta
    36061 ccgcttcgtg ccaaaccgct tcgttcccgc gcggcaggcg tttgtcgggg ctttggcaac
    36121 agcgttctgt ctggaaaccg cgcgttccct ctttacttgg tatatgggca atttcgacgg
    36181 ctaccgctcg atttacggcg cgtttgccgc cgtgccgttt tttctgttgt ggctgaacct
    36241 gttgtggacg ctggtcttgg gcggcgcggt gctgacttct tcactctcct actggcaggg
    36301 agaagcgttc cgcaggggct tcgactcgcg cggacggttt gacgacgtgt tgaaaatcct
    36361 gctgcttctg gatgcggcgc aaaaagaagg caaagccttg cctgttcagg agttcagacg
    36421 gcatatcaat atgggctacg acgagttggg cgagcttttg gaaaagctgg cgcggcacgg
    36481 ctacatctat tccggcagac agggttgggt gttgaaaacg ggggcggatt cgattgagtt
    36541 gaacgaactc ttcaagctct tcgtttaccg tccgttgcct gtggaaaggg atcatgtgaa
    36601 ccaagctgtc gatgcggtaa tgatgccgtg tttgcagact ttgaacatga cgctggcaga
    36661 gtttgacgct caggcgaaaa aacagcagca atcttgagta tttttgaaac tgtattttta
    36721 tcctaaagac attttcctat ctgctgattc caccgtttcc tttctgtgtg ccgccataat
    36781 ttttatgata gacaagccat tcaagtttgc attgactgct ttttcattgg caatatccaa
    36841 atggctggtc gcttcatcta aaaataggat tttcggttcg caatataagg cccgcgccaa
    36901 tacgatgcgt tgtttttgtc cgcctgacag tgcgctgccc atatcgccga ttaaggtttc
    36961 atagcccata ggcatggcgg agatttcttc gtgaatcata gcaaggcagg cgcaatgttc
    37021 gatttttcct ctatccggtt ctgtatcgaa aaaacaaggg gctgtactag attagcccta
    37081 aatcccacac caatcccgca ggattttaag ctgttgagac ggtgtgccga agttaaatcg
    37141 aaattcgcat tctttcaaga acagcgggaa agatttgcaa tcgattccgt tgtattttcg
    37201 caagacacgt ttagtctaga gtctgtatat tacatttatt tttagggtct gctagccaat
    37261 ttcttgttcc cttcattatt ttatcttctg aaagaaaatt atttttttcc atgctattaa
    37321 tattaatgat atgattttta tttaaaataa tttttccata taaaatttcc ggtttgtaat
    37381 agcaagagtc tgaagcaaat ccttgcccgt caatataaga aaaataaatg ttatcatcct
    37441 ccttataata ttctaaaacc aatccggata ttattttatc aaaattccct tcatttccat
    37501 ctttcatgac taaaatataa ttagcaggcc aacctttatc gtaaacatat tcaaactgat
    37561 agttcccgaa actctcgata tccgaactaa aaaagaagaa aagcaaatta atagccaata
    37621 caaaaacaaa taagaacaaa aatagcaaaa ttttcaactt agttaacaat atttacctct
    37681 cctttaaatt caatcctgaa aggtacccct taccctgggc taccaattat agtttccata
    37741 tttctaaata ttgtttttac attacttttt tctcccccca aaggaatgca ttttaaaatc
    37801 atgcttttca ggtgctaatc gatacttacc attaacatct ttaatcacag atatatttcc
    37861 atgtatagcc caacgtgaaa aatctgagta ttatatacag ttatacctta ctgaaacaaa
    37921 gctagctgta catatctgta ccatattttt actaaaaaca aaaaatattt ataattatta
    37981 aggataagat aaaaaccccc aacgccagat taattcttaa taatttcaaa taaaaaatat
    38041 ttgagatttt ttattttcca aataatatat tgaaatattt aaagttaagg tagcttgatt
    38101 taattagaaa atcaaaaaaa ccattaatta atttttcttc tttaaatatt ttatatgata
    38161 ttataaaatt aaataacatt gcgaaaacat atgaaatcat caaaatacca aatatatagt
    38221 gggaatgcat tattatttag ctccttcttt tggctttgac aaattgacag tccattgata
    38281 tccaatgctt gcatttgcaa ttgctgacgc acctgcccct gttttccctg ttagtatcaa
    38341 tggttctttt cttgcaagat gtgcacctgc acaaccacct aatactaaac caccacctat
    38401 tgtaaatcct tcctgttgta tctctaatga taacattttc ttctcgtgcc gcccttaata
    38461 cagattgtgg aattggattg acctgagcag gaacttctaa aataagtttt cctgtaagtt
    38521 ttcttcctct tagcggagca ggtgtacttg gaacattgga aatgacaaag tgagtgcgca
    38581 aagccacctg aaggcgatgt gtttgaacct gttgaaagcg gccaacaggc taaatgcgcc
    38641 cgctgccgcc taaaaggcag cccggatgcc tgattatcgg gtgtccgagg aggattaagg
    38701 gggtatttgg gtaaaattag gaggtatttg gtacgaaaac agccgaaatc ctgtgtttgg
    38761 gtttcggatg tcggggaagg gcttttttgc aaaggtctca actcatgtta ttgcaaagat
    38821 ctcatgcctt gttttgccag caggttgagt tcttctccgg caatcagcgt ggtaaagccc
    38881 cgccacaaca cagatctatg tactgtggaa gggcttcaat ttatagcttt cgaaaatctt
    38941 tcagctttaa gacggcctaa agttgtaatc tataaaacag gagctcagag ttttacggct
    39001 cactctcgct tcgcgttacc aaataccagc ttatcggttt tgaacgtata gtaatctatc
    39061 caatcctgca gatattcata cgggtaagac aagtcatact gtttcataaa atcgaagatg
    39121 tcttgatatt cgtctgccgg cagcggatcg taaacaatca aatccctcgg tgagatttct
    39181 tgatctatgc ccaaatcgaa accgtgcatg gatgcgattt tggcgtaggt aacgatttgc
    39241 ggctgcaggt agaaatcgaa ataggacaat gtttctttgg cagccatacg cgcggttttt
    39301 ttatcgaaaa gcggctctaa ggcggctttc attgcctcgg catcctgttc ggcgaaagcg
    39361 aggaagaagc ggtaatcgta caaccgcttt tgcagccatt tgctaggggt gggatgcgcc
    39421 aagactttct cgctacgttg tttcaaccga tcaagctgct taccctccac catcagcaga
    39481 gtattgtaaa tcatatgccg gttgaggtcg tagcggttta taaaggcttc tgtatcgttg
    39541 gcgatgttgt cgatattgcg caccagaaac tcacgcagct gtgggctgtc gctcatcagc
    39601 atcagaaaca tcggattttg aatgttgagc atgtcacagg gaaagaagaa gggttcaggg
    39661 tctgtagaat ttacactcag caaaaccccc agctttcctg caacataagc atattgcttg
    39721 aatttcttaa gatcatgttc aaatagatag gcatgggaag ctgcagctct aacgtccatg
    39781 aaaatatgtt gcatagctgc aagcggaacg ccttttcttt catctacata atctatagag
    39841 tcaaggcaac cgctattgaa attagcagta ttgcctatga ttacattagt aatatgctca
    39901 taccattttt gggtggtcat catattgtgc cccattgtta tctccttata ttggttttag
    39961 aaggaacttt gacaggaaga ataacggcct tacctgtttg acgatcaacg cctgctattg
    40021 ctgtttttaa tgtgccgttc ttatttgctt tgaagacagc agctttagcg ggactaccat
    40081 cgggtaattg atctaaaact tgtctaatcc aatcctcact catttgcgta tatccacccg
    40141 caccattcgg attcagctgt accgtaccgt tcctaatctg cttactttct acaatcaaaa
    40201 cgacactacc atcggcagcc tgccatacat gatcaaaacc gttatttccg ccgtatttgc
    40261 cgcccgaaag cactctgaag ccattttgtt tagctaaaga gtcaaataac tgctcggtca
    40321 ttttacctgt cgtgtccctt ccttcgccaa tagctttcag caattgtttg tcagttgctg
    40381 cagtaaccct taattcagtt tgaccgattt tgattttggt tttgctgaca tagccctgtg
    40441 cagtggcaac ggttgctgcc ttgtccaagg cttttttcat gccttgtaaa ttttttgccg
    40501 ctttcgctac tttataggcc tgtatcgatt caccgatacc cggaaccaca cccagcaaag
    40561 caaacaggtg atcggcagcg gtctgtgctt gtacaaaact ctgtatatca ccgataattg
    40621 gaataaatcc tgtctgaaaa agcaaaactt ctcgatattc ttcaggattt tcacgtatcc
    40681 atttttgttg ttccttttga aattcatcac actttgcacc aacgcatcta ggatacaaag
    40741 tattattctc caccgctatc tgtgccgcat tcgccgctgt attcacatcc ccgcctacaa
    40801 cggcagacgc agtgccggca accagttttg aataggcagt aatcttcgct ttagcttttt
    40861 cgatttcggt cgcactcata cgactgaaat cagtattctt aaccaaagcc tcaccaacaa
    40921 tctcaccgac tgcagcgcct atcgcaccat cctgacactt gcccttattc gccgccgctg
    40981 ccgcacagcc cgctatggca tgggcaatct tgtgggtggt atatcgctgc cagccctctc
    41041 tgttgataat cttgcgtatg attacgccgt ttgatggcta atcgtaccta cgcaggctta
    41101 aaatttccat caatccatat catttgagtt ttcatcttca taatatggat gatcaaacaa
    41161 atctccttcc caaatacatt cactcgttat accttgggat tccatgaaag gaacataaga
    41221 ataaaaatct ttttcatatt ctccataaaa tatattcata cctttgagcc atactgcatc
    41281 gcattcatca cacagaataa tttcatcatg aagatattta ggtttcgaat ggtagagata
    41341 gccttgttga caagcaggac acattttcat tctcttattc atgattattc catataatca
    41401 atttattttt gtgggtaagc aatagtgatt tgattgctgc ctttagtctg tacaacaata
    41461 cgtatatagc aatctgataa tttcctggtt tttatcagct ttatcctcag attccaaacc
    41521 caataataaa agccgtttga aatttttttc agacggcctt aaaacttaat taaataatgt
    41581 ccggatttaa caccaaatca tattcaccaa actttggaaa tagaattacc ttatcttttt
    41641 gggaatataa ttctgttttt tcatatcgct cattattaat aaaatcttgt ttcaaaacat
    41701 agcaatcatc cgtattttca ttgaagaaat aagtatatcc ttctgctaag tcatgagatt
    41761 ccaaagttat ataatctctc catacagcca tagaaagaaa ataataattc gaattggaat
    41821 atttttgaaa ttgatgggtt aaataatccc ttccaaattt atcgaaaaaa tttaccgaaa
    41881 aaaaacctac ttcctctcgc aaggtaatta catattcagg ttgtgtcgcc gaacctatta
    41941 cggcagtata taactcccct ttaagatgtt tttcctcagc ttgtttctca gaaaaaggct
    42001 tatgaagtct cttataacca taagaccact gctcacaata aaaaatatct ttcttcattt
    42061 gtaaatcctt cctgttgtat ctctaatgat aacattttct tcttgtgccg cccttaatac
    42121 agattgtgga attggattga cctgagcagg aacttctaaa ataagttttc cttcaagctt
    42181 tcttcctctt atcgtagtag gtgtacttgg aacattggaa atgacagtac cagggctata
    42241 ttttctaaca gcctccttga tataaccaat ccccgtactt tcttggattt gagaaaattg
    42301 ggtaaatttt cttgaaataa tttcccttgt cgcaggatca tacgaatcta ccctaaaata
    42361 ccccgtcgcc gatttaggat tggctacata aagctcatta taagggtatt ttgatgacat
    42421 gatacggtta aattcattgc cgttgtttat cctgattcta taaattggtt caacagcaaa
    42481 gcctctggat tcccttaatt gattataata ttgcctgtat gtttgtacat catgtcttgt
    42541 ccacggctct ccaggagtcc tcagaatagc aatcccgtta aatttcggat ccagccttcg
    42601 gatttgattg gtaatggcct ggatttcaag aatggcatat tcatgttcca attcttgtcg
    42661 cggagtccaa cgtatattta ccctcctgcg agctaaaaga ctattattct ccactgccac
    42721 agtagccgca ttcgccgccg tattcacatc ccctttaacc aatgccactg cgctgcctgc
    42781 gataatctgc gagtaggcta tgactttttg gcgttcttgg ggtgacagtt tgcctacatc
    42841 gcgtccgtcc aacagggttt ctcccaccat ctcgccgact gccgcgccga ttgcgccgtc
    42901 ccgacatttg cctttatttg ctaccgccga tgcacagcct gctacggcat gggctatctt
    42961 gtgggcaatg tagtcttcgc tgagattaaa tttgattttg ctcgctactt ctccgtgtac
    43021 ggtactgact atcgcaccca gtgcggcatc gcccaagttg tctttcaggc tgccgccgtt
    43081 gatggcggta tggacacttg cggcagcggt gctgttgatc aggttagcgg tcagtttgcc
    43141 tgctgcggga ctgtgaaaat gtttgctgac ggcttcggct gcttgggtgt tcagcccgct
    43201 tatgccctgc agtacgcctg cggttacggc ggcggtggcg gcctgtctga cggtgctgct
    43261 tttgcccagt tctttcaggg tatggtttat gtctcctttg ttgttgatga gggaaactgc
    43321 ggcttggctg gcgagactgg ccagtgcggc tttgcctgcg gctgtggtga tagctgcagc
    43381 tgatgtgcct gctgctactc cggttgtggc tgccgttcct gctgctactc cagttccggc
    43441 tgccgttcct gctgcggctc ctcccgctcc tgcgcctgcc gcgcccgccg ttccggcggc
    43501 tgccggggcg gacagtgcac cgtaggtcaa tacggttacg acgataacga cgacagctgc
    43561 tgctgcgggt gtcatgccct cctgtttgta gtcccattta tcgtaagcaa gctgcacctg
    43621 attccagttg atgtttttcg caacttgaag ttgtttcaaa taagcatact cgggctgctt
    43681 ggtgagggtt tcgatttggg ttttcagatt gcctttcgga atgtcgacaa tgtaaccgcc
    43741 gggtgcggac agtacgggcg caacgggacc ggtgaaactc ggcaattgca aggtttcgat
    43801 gttactgccc cgtcctgcct gtttttgcca tagagtagat ttgctgctgc tcacggtttc
    43861 tgtgtggatg ctgcttttga t
//
LOCUS       DAWFLT010000007        42531 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWFLT010000007 DAWFLT010000000
VERSION     DAWFLT010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 42531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42531
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.007"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            104..1915
                     /gene="typA"
                     /locus_tag="WGR74_000337"
     CDS             104..1915
                     /gene="typA"
                     /locus_tag="WGR74_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ0904831.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(2480..2570)
                     /locus_tag="WGR74_000338"
     tRNA            complement(2480..2570)
                     /locus_tag="WGR74_000338"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2534..2536),aa:Ser,seq:gga)
     gene            complement(2609..2695)
                     /locus_tag="WGR74_000339"
     tRNA            complement(2609..2695)
                     /locus_tag="WGR74_000339"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2659..2661),aa:Leu,seq:cag)
     gene            complement(2731..2817)
                     /locus_tag="WGR74_000340"
     tRNA            complement(2731..2817)
                     /locus_tag="WGR74_000340"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2781..2783),aa:Leu,seq:cag)
     gene            2906..5281
                     /gene="rnr"
                     /locus_tag="WGR74_000341"
     CDS             2906..5281
                     /gene="rnr"
                     /locus_tag="WGR74_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ0904832.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERRMRGIMHGDIVTVRPAGMDRRGRREAVVLDIVERAQ
                     SKVVGRFYMDRGVAVLEAEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAAKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIGHPFKAQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCSADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALVEQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAEGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESGKKAKKPVPIKVKKRKGKS"
     gene            complement(5620..7083)
                     /gene="guaB"
                     /locus_tag="WGR74_000342"
     CDS             complement(5620..7083)
                     /gene="guaB"
                     /locus_tag="WGR74_000342"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ0904833.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVAGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGADVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(7223..9781)
                     /gene="glnD"
                     /locus_tag="WGR74_000343"
     CDS             complement(7223..9781)
                     /gene="glnD"
                     /locus_tag="WGR74_000343"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ0904834.1"
                     /translation="MPANLSSALETFKRQRDAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWVEHFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTVDKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNANRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNPENRRRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIIGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTGEESDLLAWLVENHLLMSAVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETQSRSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSPDLNNPKIQSSLKQTLLEQLS"
     gene            complement(9829..10149)
                     /locus_tag="WGR74_000344"
     CDS             complement(9829..10149)
                     /locus_tag="WGR74_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0904835.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLKLMTNSADTRQMPS
                     ESGI"
     gene            complement(10474..10947)
                     /gene="bfr"
                     /locus_tag="WGR74_000345"
     CDS             complement(10474..10947)
                     /gene="bfr"
                     /locus_tag="WGR74_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0904836.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLEKEKEKHEALI
                     AAISVAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(10975..11439)
                     /gene="bfr"
                     /locus_tag="WGR74_000346"
     CDS             complement(10975..11439)
                     /gene="bfr"
                     /locus_tag="WGR74_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0904837.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(11902..12885)
                     /gene="lipA"
                     /locus_tag="WGR74_000347"
     CDS             complement(11902..12885)
                     /gene="lipA"
                     /locus_tag="WGR74_000347"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ0904838.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(12878..13501)
                     /gene="lipB"
                     /locus_tag="WGR74_000348"
     CDS             complement(12878..13501)
                     /gene="lipB"
                     /locus_tag="WGR74_000348"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ0904839.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGTEMTQIADFVQPCPTPDEVAAKLTAHLATQFTPKADNNE"
     gene            complement(13503..13778)
                     /locus_tag="WGR74_000349"
     CDS             complement(13503..13778)
                     /locus_tag="WGR74_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ0904840.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            13963..15033
                     /locus_tag="WGR74_000350"
     CDS             13963..15033
                     /locus_tag="WGR74_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ0904841.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFTGSFYRGR"
     gene            complement(15140..16087)
                     /locus_tag="WGR74_000351"
     CDS             complement(15140..16087)
                     /locus_tag="WGR74_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ0904842.1"
                     /translation="MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(16102..16509)
                     /locus_tag="WGR74_000352"
     CDS             complement(16102..16509)
                     /locus_tag="WGR74_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ0904843.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIIYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIIANP"
     gene            16590..17249
                     /gene="ung"
                     /locus_tag="WGR74_000353"
     CDS             16590..17249
                     /gene="ung"
                     /locus_tag="WGR74_000353"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ0904844.1"
                     /translation="MDTWHDALGNEKRQPYFQEILNAVRQERLSGQIIYPPAEDVFNA
                     FRLTAFDRVKVVILGQDPYHGAGQAHGLAFSVRQGVRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            17246..17455
                     /locus_tag="WGR74_000354"
     CDS             17246..17455
                     /locus_tag="WGR74_000354"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0904845.1"
                     /translation="MNADIAVAAGVLKSRLICNFHLLGKTLWQSKKNQLYASLWAGCD
                     ELRGGMDASLYKELCAYAIVFKICF"
     gene            17352..17999
                     /locus_tag="WGR74_000355"
     CDS             17352..17999
                     /locus_tag="WGR74_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0904846.1"
                     /translation="MHHFGRAATSCAAVWMRAFIKNYVLTLLFLKYVSDKHKYGGGMI
                     ELHAGTTFDDIVKLKNTADIGDRLNKIIAQIAEANDLKGVIDVTDFNDEDKLGKGKEM
                     IDRLSRLVGIFKKLNLSSNQAEDDDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSRI
                     MAKIIGISADCRPAPAFMTRPAARVRCCSKPPPKPAAKSAFTVRKKMWQPRPLPV"
     gene            18072..18545
                     /locus_tag="WGR74_000356"
     CDS             18072..18545
                     /locus_tag="WGR74_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEZ0904847.1"
                     /translation="MKTTGLRPSILPLPIRLIPPEKNGDYAFLLHLLKSLKPSGKGAI
                     ILPHGVLFRGNAEARIRTELLNLDLIKGIIGLPANLFYGTGIPACIIVIDKEHAQTAQ
                     FAEEGTNQVISGGSVFMIDASRGFIKDGNKNRLREQDIHKIIDTSTNLVTAVWCI"
     gene            18533..18847
                     /locus_tag="WGR74_000357"
     CDS             18533..18847
                     /locus_tag="WGR74_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HEZ0904848.1"
                     /translation="MVHLSEIAAQDYNLNLPRYIDSGEVEDLQNLAAYLYGGIPAHDM
                     DALEAYWQVLGRMKNELFAEHDGRFTTIQRNRLQIFPTLNSLKSCGIGVEFRANLVQP
                     QI"
     gene            18889..19833
                     /locus_tag="WGR74_000358"
     CDS             18889..19833
                     /locus_tag="WGR74_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0904849.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAAIQPGRLIRKWSESLLD
                     AFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEDANLTV
                     VFEETETGKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERLSQEL
                     ENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMTQEKA
                     AKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQQLIS
                     RLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            19975..20109
                     /locus_tag="WGR74_000359"
     CDS             19975..20109
                     /locus_tag="WGR74_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HEZ0904850.1"
                     /translation="MTHLLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT
                     "
     gene            complement(20399..20914)
                     /locus_tag="WGR74_000360"
     CDS             complement(20399..20914)
                     /locus_tag="WGR74_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904851.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CLSTDLPPIGNYSSERYIPPQTSEPTPSPSNGGQAVKYKAPVKTVSKPAKSNTPPPQQ
                     APSNNSRRSILETELSNERKALVEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(21380..23299)
                     /locus_tag="WGR74_000361"
     CDS             complement(21380..23299)
                     /locus_tag="WGR74_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0904852.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGKITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYNPEHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIAVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRAE
                     KEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSQLAKVKTQLAQIEEVWLACQEE
                     LERIETEIEKQFAER"
     gene            complement(23376..23771)
                     /locus_tag="WGR74_000362"
     CDS             complement(23376..23771)
                     /locus_tag="WGR74_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEZ0904853.1"
                     /translation="MMRRRGKKMLFEENPIDGQFAEYECGAGGIRLTGQSFHKPVLVH
                     KDSVCLSQCRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGIS
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            23944..25251
                     /locus_tag="WGR74_000363"
     CDS             23944..25251
                     /locus_tag="WGR74_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ0904854.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            25244..25678
                     /locus_tag="WGR74_000364"
     CDS             25244..25678
                     /locus_tag="WGR74_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904855.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLTVDYCKCMWEQPLD
                     RLSEKQISSFGKLGAQEQLDLLGGANAFETRDKQCVADLKSE"
     gene            complement(25737..26006)
                     /locus_tag="WGR74_000365"
     CDS             complement(25737..26006)
                     /locus_tag="WGR74_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ0904856.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(26188..28650)
                     /gene="lon"
                     /locus_tag="WGR74_000366"
     CDS             complement(26188..28650)
                     /gene="lon"
                     /locus_tag="WGR74_000366"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ0904857.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEADIKKAGMTKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDTDHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELLVEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLASDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFSKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNFKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGAEAVAESASKPKPRSRATKH"
     gene            28735..28926
                     /locus_tag="WGR74_000367"
     CDS             28735..28926
                     /locus_tag="WGR74_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HEZ0904858.1"
                     /translation="MEFDAMPSETFRLQTAWAWKDKAGTKNCSVLPLLAVPSVWFAVL
                     PPYFQNMTQVFHVFFYFSR"
     gene            28901..30004
                     /locus_tag="WGR74_000368"
     CDS             28901..30004
                     /locus_tag="WGR74_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0904859.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPSDFLDKAFAQAGLRVADVL
                     KTKPVHKKAADPNNPLAFARNRETTFLYRLKKA"
     gene            complement(30076..31821)
                     /gene="recD"
                     /locus_tag="WGR74_000369"
     CDS             complement(30076..31821)
                     /gene="recD"
                     /locus_tag="WGR74_000369"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ0904860.1"
                     /translation="MERQTDEFAQAAARAAIRFLEHYAGSGDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     DTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGKDENLPNIALAAPTGKAAAHMARALHRAINGFDALEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPFDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCRHVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEETFRQAAATVKTR
                     QTALGSMLERVFSQG"
     gene            complement(31889..32584)
                     /gene="lolD"
                     /locus_tag="WGR74_000370"
     CDS             complement(31889..32584)
                     /gene="lolD"
                     /locus_tag="WGR74_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ0904861.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVLVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAEAAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            complement(32577..33824)
                     /locus_tag="WGR74_000371"
     CDS             complement(32577..33824)
                     /locus_tag="WGR74_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ0904862.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQIRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTAKLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            34030..34308
                     /locus_tag="WGR74_000372"
     CDS             34030..34308
                     /locus_tag="WGR74_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904863.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(34364..34981)
                     /gene="recR"
                     /locus_tag="WGR74_000373"
     CDS             complement(34364..34981)
                     /gene="recR"
                     /locus_tag="WGR74_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ0904864.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVDHCARCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(35048..36586)
                     /locus_tag="WGR74_000374"
     CDS             complement(35048..36586)
                     /locus_tag="WGR74_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ0904865.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(36640..37014)
                     /locus_tag="WGR74_000375"
     CDS             complement(36640..37014)
                     /locus_tag="WGR74_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ0904866.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQRPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKVLSSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YASFSEETYDGIFNRQAPCRQG"
     gene            37164..38792
                     /locus_tag="WGR74_000376"
     CDS             37164..38792
                     /locus_tag="WGR74_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ0904867.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNATMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQDGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            38864..40117
                     /locus_tag="WGR74_000377"
     CDS             38864..40117
                     /locus_tag="WGR74_000377"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ0904868.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYKFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVRKVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRSQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
     gene            40340..40648
                     /locus_tag="WGR74_000378"
     CDS             40340..40648
                     /locus_tag="WGR74_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904869.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            40730..41761
                     /gene="ruvB"
                     /locus_tag="WGR74_000379"
     CDS             40730..41761
                     /gene="ruvB"
                     /locus_tag="WGR74_000379"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ0904870.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQH
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLATIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDVQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(41784..42512)
                     /gene="rpe"
                     /locus_tag="WGR74_000380"
     CDS             complement(41784..42512)
                     /gene="rpe"
                     /locus_tag="WGR74_000380"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ0904871.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPYTLEKIRQV
                     RAMLDRYEEKSGRRIAIEVDGGIKTDNIAAAAQAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
BASE COUNT         9705 a        11158 c        11393 g        10275 t
ORIGIN      
        1 gaaaggcaag ggcttcagac ggcatcttca tttcccaaat actgtccggt aaagcgtggt
       61 ataatgcgca gttatctctt acagtttttg aaaaacatta attatgaaac aaatccgcaa
      121 catcgccatc atcgcccacg tcgaccacgg caaaaccaca ttggtcgacc aactgctgcg
      181 ccaatccggc acattccgcg ccaaccagca ggttgacgag cgcgtgatgg acagcaacga
      241 cctcgaaaaa gaacgcggca tcaccatcct cgccaaaaac accgccatcg attacgaagg
      301 ctaccacatc aatatcgtcg acacgccggg acacgccgac ttcggcggcg aagtagagcg
      361 cgttttgggg atggtggact gcgtcgtctt gttggtggac gcgcaggaag gcccgatgcc
      421 gcaaacccgt ttcgtgacca aaaaagcctt ggctttgggg ctgaaaccga ttgtcgtcat
      481 caacaaaatc gacaagccgt ccgctcgtcc gagctgggtc atcgaccaaa ctttcgagct
      541 gttcgacaac ttgggcgcga ccgacgagca gttggatttc ccgattgttt atgcttccgg
      601 tctgtccggt ttcgccaaat tggaagaaac cgacgagagc aacgacatgc gtccgctgtt
      661 cgatactatc ttaaaatata cgcctgcacc gagcggcagc gcggacgaaa cgctgcaact
      721 gcaaatttcc caactcgact acgacaacta caccggccgc ctcggtatcg gtcgtatctt
      781 gaacggacga atcaaacccg gccaaaccgt tgccgtcatg aaccacgatc agcaaatcgc
      841 ccaaggccgc atcaaccagc ttttgggttt caaaggtttg gaacgcgtgc cgcttgaaga
      901 agccgaagcc ggcgacatcg tgattatttc cggtatcgaa gacatcggta tcggcgtaac
      961 catcaccgac aaagacaatc ccaaaggcct accgatgttg agcgtggacg aaccgacgct
     1021 gacgatggac tttatggtca acaccagccc gctggcgggt acggaaggca aattcgtaac
     1081 cagccgccaa atccgcgacc gcctgcaaaa agaattgctg accaacgtcg ccctgcgcgt
     1141 ggaagatacc gccgatgccg acgtgttccg cgtatccggg cgcggcgagc tgcacctgac
     1201 cattttgctg gaaaatatgc gccgcgaagg ctacgaactc gccgtcggca aaccgcgcgt
     1261 cgtgtaccgc gacatcgacg gtcaaaaatg cgaaccgtat gaaaacctga ccgtggatgt
     1321 acccgacgac aaccaaggcg cggtaatgga agaactcggc cgccgccgtg gcgaactgac
     1381 taatatggaa agcgacggca acggacgcac ccgcctcgaa taccatattc cagcgcgcgg
     1441 cttgatcggt ttccaaggcg aatttatgac cctgacgcgc ggggtcgggc tgatgagcca
     1501 tgtgttcgac gattacgcgc ccgtcaaacc cgatatgccc ggccgccaca acggcgtgct
     1561 ggtgtcccaa gagcagggcg aggcagtcgc ttacgccttg tggaatctgg aagaccgcgg
     1621 tcgtatgttc gtatcaccca acgacaaaat ctacgaaggt atgattatcg gcatccacag
     1681 ccgcgacaac gatttggtgg tcaacccgct caaaggcaaa aaacttacca acatccgcgc
     1741 cagcggcacc gacgaagccg tgcgcctgac cacgccgatc aagctgacgc tggaaggtgc
     1801 ggttgagttt atcgacgatg acgaactcgt tgaaatcacg ccgcaatcca tccgtctgcg
     1861 caagcgttac ttgagcgaat tggaacgccg ccgccacttt aaaaagctgg attgatgttt
     1921 actggattaa tgtttaaatg atacgcgatg ccgtctgaaa aatttcagac ggcatttttt
     1981 attcggacgg gctttgcggc ttcttgaagc tgtttcagac ggcgtttttc ctacccaatc
     2041 aagaaactgc cgccattttt ccagcggtat atcgccccgt cgcgtttcgg tatcgggttc
     2101 ggcttcccgg cagcatgtcg aacaatgccg tttgaaggaa tacgcctttt gagtccttac
     2161 aagcttagga agaggatata gtggattaac aaaaatcagg acaaggcgac gaagccgcag
     2221 atagtacaga tagtacggaa ccgattcact tggtgcttca gcaccttaga gaatcgttct
     2281 ctttgagcta aggcgaggca acgccgtact ggtttttgtt aatccactat aaaatatacc
     2341 gcaaaacata ccataaaaaa aacggtaacg gatagttgta agcggttgat gacgattata
     2401 gacaataacg ggttttccca atgaaattat tgtttgaata ataaaaaatc ccaaaccgta
     2461 aaagtttggg atttgtattt ggcagagagg aagggattcg aaccctcgat acgctattca
     2521 cgtatacacg ctttccaggc gtgcgactta aaccactcat ccacctctct aatggcggaa
     2581 attatcccaa tcgggataat ttattatttg gtgcccggga gaagactcga acttccacac
     2641 ccatgaggat accagcacct gaagctggcg cgtctaccaa ttccgccacc cgggcaatct
     2701 aaatattgaa ataaagcaaa gcatttgatt tggtgcccgg gagaagactc gaacttccac
     2761 acccgtgagg atactagcac ctgaagctag cgcgtctacc aattccgcca cccgggcttt
     2821 gctttatttg ctgttttgcg gtacagtgtc ctgccgcaaa gaagtcgcta ttatatagtt
     2881 cataaagaga atgtcaacag tccaaatgaa taaaaatatt aaatctttaa atttacggga
     2941 aaaagacccg tttttaagtc gtgaaaaaca gcgttatgaa catcctttgc ccagtcggga
     3001 atggataatc gaattgttgg agcgcaaagg tgtgccttca aaaatcgaat cgcttgcgcg
     3061 cgagctgtcg attacggaag acgagtatgt ctttttcgaa cgccgtctga aggcgatggc
     3121 gcgggacggt caggttttaa tcaaccgccg gggcgcggtt tgcgcggcgg acaaattgga
     3181 tttggtcaaa tgccgtgtcg aggcgcacaa agacggcttc ggttttgccg tgccgctcac
     3241 gcccgccaaa gacggtgatt ttgttttgta cgaacgccgg atgcgcggca ttatgcacgg
     3301 cgatattgtc actgttcgtc ctgccggcat ggaccgcagg gggcggcggg aagcggtggt
     3361 tttggatatt gtcgaacgcg cgcaaagcaa agtggtcggg cgtttctata tggatcgcgg
     3421 cgtggcggtt ttggaagcgg aagacaagcg tctgaaccaa agcatcgtat tggaaccgga
     3481 cggcgtggcg cgtttcaaac ctgaatccgg tcaggtcatc gtcggcgaaa ttgaggttta
     3541 tcctgagcaa aaccggccgg cagtggcaaa aatcatcgaa gttttgggcg attatgccga
     3601 cagcggcatg gagattgaaa ttgccgtgcg caagcatcat ttgccgcacc aattcagtga
     3661 agcgtgtgcc aaagccgcga aaaaaattcc cgaccatgta cgcaaaagcg atttgaaagg
     3721 ccgcgtcgat ttgcgcgatt tgcctttggt aacgatagac ggcgaaacgg cgcgcgattt
     3781 tgacgatgcc gtgtttgccg agaaaatcgg acgcaattac cgtctggtcg tggcgattgc
     3841 cgatgtcagc cattatgtcc gccccgatga cgctatcgac acggacgctc aggaacgcag
     3901 caccagtgtt tacttcccgc gccgcgtgat tcccatgttg ccggaaaacc tgtccaacgg
     3961 catctgctcg ctcaatccta atgtcgagcg tttgtgtatg gtgtgcgata tggtcatcac
     4021 ttacgcgggc aatattaaag aataccgctt ctaccccgcc gtgatgcgct ctcatgcccg
     4081 cctgacctac aaccaagttt ggaaatggct ttcaggcggc atcgggcatc cgttcaaagc
     4141 ccaaatcgac acgctttaca aactcttcaa aatccttcag aaaaagcgtt tcgaacgcgg
     4201 ggcggtggag tttgacagca tcgaaaccca aatgcttttc gacgacaacg gtaaaattga
     4261 aaaaatcgtc cccgttgtcc gcaacgatgc ccacaagctg attgaagaat gtatgctggc
     4321 ggcgaatgtt tgctcagcgg attttctatt gaaaaataag catacggctt tgtttcgcaa
     4381 ccatttgggc cccacgcccg aaaaactcgc caccctgcgc gagcagctcg gtctgttggg
     4441 gcttcaactt ggcggcggcg acaacccgtc gccgaaagac tatgccgcgc ttgtcgaaca
     4501 attcaaaggc agacctgatg ccgaattgct gcaagtcatg atgttgcgct ccatgcagca
     4561 ggcggtttac gaaccgcatt gcgacggaca ctttggtctt gcctacgaag catacgccca
     4621 cttcacctcg cccatccgcc gctatcccga cctgaccgta caccgcgcca tcaaagccgt
     4681 gttgaatcag caaacctaca cgccaaaaaa aagctggcag gctttgggcg tgcatacctc
     4741 gttctgtgag cgccgtgccg acgacgccag ccgcgacgtg gaaaactggc tgaaaaccta
     4801 ttatatgcgc gataaggtcg gcgaagtatt cgaaggtaaa atctccggca tgaccagttt
     4861 tggtatcttt gtaacactgg acggcatcca cattgacggc ttggtgcata tcagcgattt
     4921 gggcgaagac tatttcaact tccgccccga aatcatggca atcgaaggcg aacgcagcgg
     4981 catccgtttc aacatggggg acagggttgc cgtccgggtc gcccgtgccg atttggatga
     5041 tggaaaaatc gattttgtcc tgattgccga ggggagcggc agggggcgga aagttaaatc
     5101 atccgcgtct gccaaaccgg cagggacggc ggggaaaggg aaaccgaaaa ccgccgccga
     5161 gaaaaaaaca gcccgaggcg gcaaagtaag gggaaggggc gcgtctgccg ccgcagaatc
     5221 ggggaaaaag gcaaagaaac cggttccgat taaggtaaaa aaacggaaag gcaaatcata
     5281 atgctgacgg ggtggcttgg ggagggcggg gcatactttc ccgaagccgc cgcccgattt
     5341 tttgatatgg cggccggcgc atcatttcag gccaatcgaa acgcccgtat tgaaagattg
     5401 cgtttatttc caccgccgtt ttaaaggccg acggtattcg gcagacgggg cgcaaacggc
     5461 gttcagacgg cattttcatt ctttcggcgt gtccgtccga attgctttgc ccgtccgcgc
     5521 aatcagcccg tgcggcttgc cttgaacgga caaaaaatgc cgtctgaaac ccgaaaatca
     5581 ggtttcagac ggcatttcgc cttggaaagg ctgttcaaat cagcggtggt aattcggtgc
     5641 ttctttggta atttgaacgt cgtgaacgtg cgattcgctc atacctgcgg aagtgatttc
     5701 cacaaattcg gctttttcgt gcatttcggc aatattggcg caacccaaat aacccatact
     5761 ggaacgcaaa ccgccggtca gttggtggat gatgttcaca atcggacctt tgtaaggaac
     5821 gcggccttcg atgccttcgg ggacgtattt gtcggtgctg tcggttttgt cttggaagta
     5881 gcggtcggca gaaccttggc tcatcgcgcc caaggaaccc ataccgcgat aggatttgta
     5941 cgagcggcct tggtagagtt cgatttcgcc cggtgcttct tccgtgccgg caaacatacc
     6001 gccgagcatg acgctgtacg cgcctgcggc gagggctttg gcgatgtcgc cggagaagcg
     6061 gatgccgccg tcggcaatca gcggaacgcc cgtgcctttg agggcttcgg caacgttgtg
     6121 aatggcggtc agttgcggca cgcccacgcc tgccacgata cgggtggtgc aaatcgaacc
     6181 cggaccgata ccgactttga cggcatccgc gccggcggca accaaatcca aagcggcttt
     6241 ggcagtggcg atattgccgc cgatgacttg gatgtgcgga taagtttctt tgacccaacg
     6301 cacgcgatcg attacgcctt ggctgtgtcc gtgtgcggta tcgacgacaa ttacgtccgc
     6361 gcctgcttcg accaaggctt tgacgcgttc ttcggtatcg ccgccggtgc cgactgccgc
     6421 accgacgcgc agacggcctt cggagtcttt gttggcattg ggaaattcgg tggtttttaa
     6481 aatgtcttta acggtaatca agcctttaag ctcgtctttt tcgttcaaga ctaaaacgcg
     6541 ctcgacttta tgcgtgtgca tcagttcgcg cgcttcgtct atgcttgtgc cttcggggac
     6601 ggtaaccaag cgttcgcgcg gggtcataat ggcggaaacg ggcaaatcga cgcggttttc
     6661 aaaacgcagg tcgcggttgg ttacgatgcc ggcgactttg ccgttttcaa cgacgggcag
     6721 gccggacatt ttgcgtttgc gctgcgcgcg catttccaag acttcgcgga tgagcgttgt
     6781 cggcgcaacg gttacggggt ctttgaccac gccgctttcg tggcgtttca ctttggaaac
     6841 ggcgcgagcc tgcatctccg gcggcatatt tttatggatg atgccgatgc cgccttcctg
     6901 tgccatcgaa atggcgaggc gcgcttcggt aacggtgtcc atcgcggcgg aaagcagggg
     6961 gaggttgagc gtgatttcgc gggtgagctt ggtttgaagt ttaacgtctc gcggcagcac
     7021 ggttgagtgt gcgggaacca acaaaacatc gtcgaaagta taggcttttt ctacgatacg
     7081 cataatgctc ggtctttcag tttgtgcaag atgcacggca ttatagcacg ttgccggcgg
     7141 cttgacagtt tatattgttt gcaatatccc cgccttcagg cggcatacgg cgcggcggcg
     7201 gaatgccgtc tgaaggcggg cgttatgata attgttccag cagcgtctgc ttcaatgagg
     7261 actggatttt ggggttgttc aaatcggggc tgaaaacggt aaaactgtct tcggcgcgtt
     7321 cgtccaacgt ggagattttg gcgtagcgca ggctgacgtt gtgggcgaaa aagacttccg
     7381 ccatatcggc gagcaggaag gggcggttga cggcggtgat ttcgacggaa taccagtcgg
     7441 gatagtcttc ttcgggggtg atggtgatgc tcggtgcgat cggcatatag cggctgcggc
     7501 ggctgatgcg gcggctgcgg ctttgggttt cggcaacggt gtgtccgtgg ataaagctgt
     7561 tgagttcggc ttcgagcgcg ctttggatgt cggggtagtc ttcgggggcg tgctgcgagg
     7621 ggatttgcac gatgaaggtg tcgaggatgt agtcgtgttc ggtgatgaag gcgcgggcgg
     7681 cgaggatgtc gaagccgtgg cggctgaaga tgcggcagag gcgggcgaac aggcgcgggc
     7741 cgttgggcat gaaaaccatg acttgaaagc tgtcgctttt gggcaggatg cggctgcgga
     7801 cgatgggggt ttcaaagtcg tggactaggt tggcggcgtg ccacaggatt tcgcgggact
     7861 ggtggcgggc gaagtaggcg gaaccgagcg cgttccatag ttttttctgc tgtttttcgg
     7921 ggacggcggc gcgggtgagt aagtcggcgg cttcctgccg gcggcggccg aagagggtgt
     7981 gcgggttgcc gccgttgcct gtaaggtagc gtccggcggc atggaagagg ctttccagca
     8041 ggctggcgcg ccatgcgttc cacagcttgg gattggtgcc gcgtatgtcg gaaatggtca
     8101 gaaggtagag cgcgctgagg cgttcgtggg tttggacgcg tttgcagaaa gcatcgagta
     8161 cgtcggggtc ttggatgtct tctttttggg cgacggcaga catcagcagg tggttttcga
     8221 ccagccaggc gagcaggtcg ctttcttctc cggtcaggaa gtggtcagcg gcaaattggc
     8281 gcgcgtctgc gatgccttgt atggcatggt cgccgccgcg tcctttggcg atgtcatgga
     8341 aaaaggcggc aaggtagagg atgtcttgtt tttcaaagga ctgcatcagt gcagaggcgt
     8401 agggcagctc gtggctgtgc atatccaagg caaggcggcg gacgttgcgg acgacggtga
     8461 ggatgtggtc gtccacggga tagatgtgga acaggtcgtg ttggagcagg ccgatgattt
     8521 tttcccacgc gggcaggtag cggcccaaca cgccgtagag gttgagaaag cgcagggtct
     8581 gggtcagccc gttgccgttg cggaaaaaac cggcgaagcg gcggcggttt tcgggatttt
     8641 ggtagaagct gcggttgatt ttgcgcgtcg cgccccacca ggcgcgcagg gtttgcggtt
     8701 cgagcgcggt aatgtcgttg cgctgctgca tgatttcgac gattttgaaa atgtgttcgg
     8761 gccgtctgaa aaaaatatcg gtgtgccgcg cggcgatttg gttgttgact tggatgtagt
     8821 cgtcgtcaat ccgcagggta acgcgcaacg gggtggagga aacgcggctt tgcagcatag
     8881 gcgtgaggat gccgcccagt tgtttgacgg ttttaatcgc gcggtaaaac acgcgcatca
     8941 gttcttcgct ttggcggcgg aggttcaagc cttcataacc catgctttcg gcgacttgcg
     9001 gctgcaaatc gaacagcagg cggtcttcgg cgcgattggc gtttaaatgc agatggatgc
     9061 ggatgtgggc gaggcggcgg tagccgtgcg aaagcatacc ggcttcggca cgcgtcaaaa
     9121 tccgctgttt gagcaggtcg ggcaggtcgg tcgccaagcc ttgcgccttc gctatccaaa
     9181 gcagggtgtg gatgtcgcgc agaccgcccg gacagctttt gatattcggc tccaatactg
     9241 cccccgaacc ttgcgatttg gcgtggcggt gttccatctc caccagtttt gcctcgacaa
     9301 acgccgccac attgcgctgc gcgttcattt tttccgccag tttatccacc gtttggcggt
     9361 tgccaaacaa aaacctagcc tctaaaaacg ctgtgtcccc cgtaatatca ttgcgcacgc
     9421 tttcacatag ttcatcaacg ctgccgcttt ttacagacgg catcagtttg cagtcccaca
     9481 gggtttgaac aaaccgggca atctgttcct gaatgccgtc tgaaagcggg gcaggggaga
     9541 caaccgccaa atccacatcc gaacagggat acagttcgcc gcgcccgaag ccgcccaccg
     9601 ccatcaggca taacgcgctg ttttgaaaat gttctaccca caatgccgcc agtaaggttt
     9661 cgactgccgc cgtatattcc ctgaaaaata ccgacacgcg gttggctttc aaataatgcg
     9721 cttcggcggc atcgcgctgc cgtttgaagg tttccagtgc tgaagacagg tttgcaggca
     9781 tttttattct ttcgattggc gggaaaaggg aggtggatgg ttcggcggtc aaataccgct
     9841 ttcagacggc atttgtcggg tatcggcgga attggtcatc agcttcaacc gttcctgcgg
     9901 cggcagcagc aactgatacg ccgccacgcc caaagccgcc gccatttttt cgatattcga
     9961 cagggcgatg ttccagcgtt tgcgctcgac tgccgacaca taagtcctgt ccaaaccgca
    10021 ttgccgcgcc aattcttctt gcgaccaacc cttgttcacg cggaaaagcc gcatattgta
    10081 tgccaatacc gcccgcaaat cctgttcgtc aggcagttcg gcaggcagag tcaatttgtt
    10141 gcccatcatt tgcttccaaa taaatggtta aagttaagca tttgctgtta cggattttac
    10201 ttcacataaa agccaaaaaa aaggggtggc ggcagccaca cccaaacaca cacacatcaa
    10261 gaggaaagag gtaacatcag gacagattgg gaaacagcaa acaagcaatc tagatttcat
    10321 atcggtatta tcaaacaaaa accgttatcg agtattgatt caaatcaatt gagatttgat
    10381 tctcaaaccg gtaaagaaaa acggcatacc cgtttatacg gatatgccgt ctgatgatgc
    10441 aggggactgc tgtatttggc agtggtttgt gttttagtcc tcttgcgccg ctgtttgcag
    10501 gtagttcggc aaaccaattt tgccgattag ttcctgctgg gtttcgagcc agtcgatgtg
    10561 ttcttcgttg gtgtcttttt gtttttccaa caaatcacgg ctgacataat cctgttgcgc
    10621 ttctgccacg gaaattgccg caatcaatgc ctcatgtttt tctttctctt tttccaaatc
    10681 gcaggcgatg atttcttcgg tggactcgcc aatcagaagc ttgcccagtt cttgcaggtt
    10741 cggcagacct tcgaggaaca gaatgcgctc gatcaaatcg tcggcagctt tcatttcaac
    10801 gatggactgt ttgaagaaat gttcgcccag ttcttcaaag ccccagtttt tcaaaatacg
    10861 ggcgtgaagg aaatattggt tgatggttac cagcagcaag cctaagtttt tgtttagctc
    10921 gcggataacc agacggtcgc ctttcatacg gactcctttt attccgaata cggattacag
    10981 ttggctttgg tagtagttgc cttcgccaat taactcgatc aggcgaagct gctgttccag
    11041 ccagtgggcg tggtcttctt cggtatcttt cagttgggca accatcaggt cgcgcgtaac
    11101 atagtcttga gcctcttcgc acagtttgat gccttttttc aacgcatcac gtacttcata
    11161 ttcggtttgc aggtcggctt tgaggcagga aaccacgtcc gtgccgatat tcagttcggc
    11221 gcgcgccatt ttcggcgtgc cgcccagcat caggatgcgg cggatgaagt cttcggcgtg
    11281 tgtggtttct tcttccatct cgtgattgag acgttcaaaa agtttggtgt agccccattc
    11341 ggagtagagg cgggagtgga taaagtattg gtcgcgtgcc gccagctcgc cagacagcaa
    11401 ttcgttcata taatcaacaa cagcttgatt gccttgcata atatctctct ttctgtaact
    11461 tggttttcgg tatgtctgaa cagcatctgt gtcgctattc gggatgcgtg cattgtatgc
    11521 aaaagctgtc ggcatgacaa attttctatt taaaatacaa acaattatca aaagaaatag
    11581 ggcgtattcc ccgatgcctt ccaaatcagt atgctgtttc ttatcggttt ttttgctgct
    11641 aaaactaaga atccatctca tccataaaga acatttacac ttattaacca tagcgaaaag
    11701 caaatactgt acggtttttt atcaaaattt ataatgaatc gttctcatta accgacagat
    11761 tcttttgaga ttatcggatt ttgggaataa attatgccgt ctgaggggct ttcagacggc
    11821 atagacaatt aaagctgatt aaacaacgcg gcagacagca gcgtaggaaa ttatttttta
    11881 cttcggaacg gtattttggg tttaatgatg cccgcaaccg ccgtggcttt cgcgtaaggc
    11941 ttcggcagcc tgttcgtcgg cgtggtagct ggaacgtacc atcgcgccga tggcggcatt
    12001 gctgaagccc agttcgtatg cttctttttc aaagattttg aactgctcgg gcgtaacgta
    12061 gcgcaagaca ggcaagtggc cgtctgaagg ctggagatac tgtccgatgg taatcatctc
    12121 gatattgtgc gcccgcatat cgcgcataat ttcgcgcacg tcttcgtctg tttcgcccaa
    12181 gccgaccatg atgccggatt tggtcgggat gtgcggcatc atttctttat aacgttttaa
    12241 taagtctaaa gaatgttgat aattggcacc gggacgggct tttctgtaca ggctcggatg
    12301 ggtttctagg ttgtggttca tcacgtcggg cggggtttcg gcaaggattt tgagtgcgat
    12361 gtccaagcgt cctcggaagt cggggacgag gatttcgatt ttggtgttcg ggctggtttc
    12421 gcggatggct ttgatgcagt cggcgaaatg ctgtgcgccg ccgtcgcgca ggtcgtcgcg
    12481 gtcgacggag gtgatgacga cgtaacgcag gttcatggct ttgacggatt cggcgaggtt
    12541 tttcggttcg tcggggtcga gcatattggg ccgaccgtgt cccacgtcgc agaacgggca
    12601 gcggcgggtg cagatgtcac ccataatcat gaaggtcgcc gtgcctttgc tgaagcattc
    12661 gccgatgttg gggcaggagg cttcctcgca aacggtgtgc atcttttgtt cgcgcaaaat
    12721 gtctttgatt tcaaagaatt tgcgcgatgg gagtttggcg cgtatccatt cgggcttttt
    12781 cagtttctcc tgaaggggga cgactttgat ggggatgcgg gcggttttgt ccgcgcctct
    12841 gagtttgatg ccgcgtttgg ggtcgtcggt tttgatttca ctcattgttg tctgctttcg
    12901 gtgtgaattg tgttgcaagg tgtgcggtaa gtttggcggc gacttcgtcc ggcgtggggc
    12961 agggttggac aaaatccgcg atttgcgtca tttccgtacc ggcgtagccg caggggttga
    13021 tgtgggtaaa cgggcttaaa tccatattga cgttgagcgc aagcccgtga tagacggagc
    13081 cgtttttgat acgcagcccc agtgaggcga ttttgcgttc tccgacataa acgccggggc
    13141 gtttggggtc tgccgccgct tcgatgccgt attctgccaa tgtggcgatg atgctgtttt
    13201 caagcgcgga aacgatgttt ctaacactgg ttttgcgccg tttgaaatca atcatcgtat
    13261 aaacgaccaa ttgcccgggc ccgtgatagg taatctgccc gccccggtcg atttggacga
    13321 cgggaatgtc gtcgcgaatc agcaggtgtt cgggttttcc cgccagtcct tgtgtgaaca
    13381 cgggcgggtg ttcgacgacc cacagttcgt cttcggtgtc ggcattccgt ccggcattaa
    13441 aggttttcat cgcttcaaaa gtcggcagat attcgaccaa acctttgtgt atgattttca
    13501 tctcaaagta ccactttgac cagttcgtgc gaagtcagcg cacggtagat gttgtccaat
    13561 tgttcttggt tttcaacctt tacctgtacg gtggcgccag tatagttgcc tttgctgctc
    13621 ggacgcgtgg tgatgtggtg cgcctgcgtg tcgggggcgt ggaggcggac ggtgtctaaa
    13681 accgcctgct cgaactcggg atgcaccgcg cccattactt tcaatgggaa ggtgcaggga
    13741 aattcgatga gggatgtttt gtttttttgt tcggtcatga tgtgctgcct tatcgtgtac
    13801 ggtatgccgt ctgaaggcgg gtttgccttt cagacggcat cggatgtgcg ttattttagc
    13861 ctaaaccgcg ataacaggct atcgggaagg cggaggcttt tttgacggcg cggcggttct
    13921 gctatactgg cgcacaatat tatttttaga agggtggttt ttatgtatcg gaggaaaggg
    13981 cggggcatca agccgtggat gggtgccggt gcggcgtttg ccgccttggt ctggctggtt
    14041 ttcgcgctcg gcgatacttt gactccgttt gcggttgcgg cggtgctggc gtatgtattg
    14101 gaccctttgg tcgaatggtt gcagaaaaag ggtttgaacc gtgcatccgc ttcgatgtct
    14161 gtgatggtgt tttccttgat tttgttgttg gcattattgt tgattatcgt ccctatgctg
    14221 gtcgggcagt tcaacaattt ggcatcgcgc ctgccccaat taatcggttt tatgcagaac
    14281 acgctgctgc cgtggttgaa aaatacaatc ggcggatatg tggaaatcga tcaggcatct
    14341 attattgcgt ggcttcaggc gcatacggga gagttgagca acgcgcttaa ggcgtggttt
    14401 cccgttttga tgaggcaggg cggcaatatt gtcagcagta tcggcaacct actgctgctg
    14461 cccttgctgc tttattattt cctgctggat tggcagcggt ggtcgtgcgg catcgccaaa
    14521 ctggttccga ggcgttttgc cggtgcttat acgcgcatta cgggtaattt gaacgaggta
    14581 ttgggcgaat ttttgcgcgg gcagcttctg gtgatgctga ttatgggttt ggtttacggc
    14641 ttggggttgg tgctggtcgg gctggattcg gggtttgcaa tcggtatggt tgccggtatt
    14701 ttggtttttg ttccctattt gggcgcgttt acaggactgc tgctggcaac cgtcgccgcc
    14761 ttgctccagt tcggttcgtg gaacggcatc ctatcggttt gggcggtttt tgccgtagga
    14821 cagtttctcg aaagtttttt cattacgccg aaaatcgtag gagaccgtat cggcctgtcg
    14881 ccgttttggg ttatcttttc gctgatggcg ttcggacagc tgatgggttt cgtcggaatg
    14941 ttggcaggat tgcctttggc cgccgtaacc ttggtcttgc ttcgcgaggg cgtgcagaaa
    15001 tattttaccg gcagttttta ccggggcagg taggcggttc cgaaacatat ttgaagcgga
    15061 atacaacctt gcccgggttt aaataaaaat gccgtctgaa ccccgaaaac gggacttcag
    15121 acggcatttt catcacggct tatttggcgg ttttgctgct gtcgataatt ttcataccgg
    15181 cagaaatcag gctgccgatg tcggcaacat tggcgggcat aatcagcgta ttgctttctt
    15241 tggcaagatt gttgaacgcg gcgacgtatt gttccgcaat cttcagattg accgcatccg
    15301 caccgccttg ggtttgaagg gcggcggcaa tttgacggat ggcttcggca ttggcttcgg
    15361 caacaaggcg caaggattcc gcttcacctt tggcgcggtt gatgcgggcg attttctcgg
    15421 catttgacgc attgaccgca gcctgagcct cgccttcgga ttgttggatt tcggcttcgc
    15481 gctgaccact ggcaaggttg atttgttcga ttttacgacc ttcggattcg gcgatacggg
    15541 cgcgtttttc gcgttcagca gtaatttgcg cctgcattga gcgaaggatt tcttgcggcg
    15601 gaaccaagtc tttaatctca taacgcaaaa ccttcacacc ccaagctccg gcggcttcat
    15661 cgagggcgga gacgacggtg ctgttgattt cgtcgcgttc ttcaaacgtt ttgtccaatt
    15721 ccatacgccc gataacggaa cgcagcgtcg tttgggcaag ctgggtaatc gccataatgt
    15781 agttgctcga accgtatgag gcgagtttgg ggtcggttac ttggaaatag atgatgccgt
    15841 caaccgtcag ctgggtgttg tcgcgcgtga tgcagacctg gctgggtacg tctaaaggga
    15901 tttctttcag cgaatgacgg taggcgacgc ggtcgataaa gggaatcaaa atattcaaac
    15961 cggccgtcaa ggcgcgatgg aaacgtccaa gcctttcgac aacgtggact tcctgctgtg
    16021 ggatgacgac aaaggatttg aagccgaaaa cggcaacggc taccaacaag ataatgaaaa
    16081 attccataat tcctccgagt gttaagggtt tgcgatgata agaaggttgc cttccttgcg
    16141 gacgattagg gcgcgagttc ctggttcaag ctcttcttgc cccgtatttt gagcctgcca
    16201 gtgcgtaccg cgataaaaaa cttcgtaacg gttgccgcct gtgtgtcgga ggatttcgac
    16261 atattgtccg gcatccaaat cctgatatga atccgtttca acttttccca cggcggtttt
    16321 ggcgtgtacg aaccaaatac ccagcgcgga aagcagagca gcggtcaaga cggcggcagg
    16381 cgtgctgccg gtcagtccgt agataatgcc cgaacccgcc aaagccgcgc tgacaaccag
    16441 aagataaacc gttcccgtca ataattcgat gattaagacg gcaacagcag caacaaacca
    16501 tacagtcata catttcccca caaagcgcgt cgtttgacaa aatcacgcga tatcagcagt
    16561 atagccgaat ttgaaaggat agggcagata tggatacttg gcacgatgca ctgggcaacg
    16621 aaaaacggca accgtatttt caggaaattt taaatgcagt caggcaggaa cgtttgtcgg
    16681 ggcaaatcat ctatccgccg gcggaggatg tgttcaacgc attccgcctg acagcgtttg
    16741 accgagtcaa agtcgtcatt ctcggacaag acccgtatca cggggcaggg caggcgcacg
    16801 gtttggcatt ttccgtcagg cagggcgtcc gcataccccc gtctttactc aatatctaca
    16861 aagaattgga aaccgacatc gaaggctttt ccattcccgc gcacggctgc ctgacagcgt
    16921 gggcggagca gggcgtatta cttctgaaca cggttttgac ggtgcgtgca ggacaggcgc
    16981 attcgcacgc ccttttaggc tgggaacgct ttaccgatac cgtcatcagg cagcttgcga
    17041 cacaccgcaa gcatcttgtc tttatgctgt ggggtgggta tgcacaacaa aaaaggaaac
    17101 tgatagacag tcaaaaccat ttgatattga ccgcaccgca tccgtctcct ctgtcggcat
    17161 atcgcggttt tttcggttgc cgccattttt cacaggcaaa cagctatttg agccggcacg
    17221 gtatcgatcc gataaactgg aagctgtgaa tgccgatata gccgttgccg ccggcgtgtt
    17281 aaaatcgcgt ttgatttgta atttccattt attaggcaaa accttatggc aatcaaaaaa
    17341 aaatcagctt tatgcatcac tttgggcggg ctgcgacgag ttgcgcggcg gtatggatgc
    17401 gagcctttat aaagaactat gtgcttacgc tattgttttt aaaatatgtt tctgataagc
    17461 ataagtacgg cggcggcatg attgagctgc acgccggtac gacttttgac gacatcgtca
    17521 aactcaaaaa caccgccgac atcggcgacc gcctgaataa gattatcgcc caaattgccg
    17581 aagccaacga cttaaaaggc gtgatcgacg ttaccgactt caacgacgaa gacaaactgg
    17641 gtaaaggtaa ggagatgatc gaccgtttga gcaggcttgt cggcattttt aaaaagctca
    17701 acctttcttc caaccaagcc gaagacgacg atttgttagg tgatgcctac gaatacctga
    17761 tgcgccattt tgcgaccgag tcaggcaaat ccaaagggca gttttacacg cctgccgaag
    17821 tctcccgcat tatggcgaag attatcggaa tcagcgcaga ttgccgtcca gcaccagcgt
    17881 ttatgacccg acctgcggct cgggttcgct gttgctcaaa gccgccgccc aagccggcag
    17941 ccaaatcagc ctttacggtc aggaaaaaga tgtggcaacc gcgtcccttg cccgtatgaa
    18001 tatgattttg cacaacaacg aaaccgccga aatcaacacc gggaacacct tgtccgattc
    18061 gtctttccgt gatgaaaacg acgggcttaa gaccttcgat tttgccgttg ccaatccgcc
    18121 ttatcccgcc cgaaaaaaac ggcgattacg cctttttgct gcatctgctc aaaagcctga
    18181 aaccaagcgg caaaggtgcg attattcttc cgcacggtgt gctgtttcgc ggcaatgccg
    18241 aagcgcgtat tcgcacggaa ttgcttaacc ttgaccttat taaaggcatt atcgggctgc
    18301 ctgccaacct gttttacggc acgggcattc ctgcctgcat catcgtcatc gacaaagaac
    18361 acgcccaaac cgcccaattt gccgaagagg gaacaaacca agttatcagt ggcggcagcg
    18421 tgtttatgat tgacgcatcg cgcggcttca ttaaagacgg caacaaaaac cgtctgcgtg
    18481 agcaagacat tcacaaaatc atcgacactt ccacaaacct cgttacagcc gtatggtgca
    18541 tttaagcgaa atcgcagcac aagattacaa ccttaatctg cctcgctata tcgacagcgg
    18601 agaagttgaa gacctgcaaa atctcgccgc ctatctttat ggcggcatac ctgcgcacga
    18661 tatggacgca ttggaagcct attggcaagt tttaggccgt atgaaaaacg agttgtttgc
    18721 cgaacacgat ggccgcttta ccactataca acggaatcga ttgcaaatct ttcccactct
    18781 caacagctta aaatcctgcg ggattggtgt ggaatttagg gctaatctag tacagcccca
    18841 aatttaatcc actataaaat cgaaagcagc caaatcaaag cccatatatt ggcgcacccc
    18901 gattacgccg ccttcaaagc cggacacctg gcaaagtttg ccgcgtggca cactcaaaac
    18961 gaccttgccg ccatccaacc gggcaggctt atccggaaat ggagcgaaag cctgctggac
    19021 gcgttcaaac ccggcagcct gattgaagaa tacgatttct accaaatcct gacggactac
    19081 tgggcggaaa ccctgcaaga cgatgtttat ctcatcgccc aagatggctg gaaggcggtt
    19141 aaaaacctgg ccgaaatcac caaagaaagc gatgaagacg cgaacctgac cgtcgtcttt
    19201 gaggaaaccg aaaccggcaa aaaaggcaaa gccaaaacca agcgcatcag caaaaaatac
    19261 cgcagcgaag tcatcgcccc cgagctggtt gcccgccgct acttttcaga cggcatcgcc
    19321 aagctggaag aaaaacaaag cgagctggaa cgcctaagcc aagaattgga aaaccacata
    19381 gaagaacacg gcggcgaaga gggtgcgctg aacgacgtat tggatgcaaa aggcaaactt
    19441 tccgccaaac ttctgaaaac cgcattggaa gaaagcggca tagaagaagg cgaacgggct
    19501 gttttacaaa ccacccaaac actgatgacg caggaaaaag ccgcgaaaga cgcagtcaaa
    19561 acccaaatcg aagccctgaa ccttgccgta ttcaaacaat tcggccgact ttccgaagcc
    19621 gaaatcaagc agcttgccgt tcaagacaaa tggcttgccg atttacaaag ccgaatcgaa
    19681 aatcgcttgg aaaacagtat tcagcagctt atcagccgct tgaacacgct ggaagaccgc
    19741 taccgcagcc cgatggccga gcttgcccga gaagtggaaa agtggcaaag caaagtcaat
    19801 gcccaccttg aaaatatggg ttttggaggc tgaaatggcg gcacagacag gctataaggc
    19861 gagcgggttt tgagaccttt gcaaaattcc ccaaaatccc ctaaattccc accaagacat
    19921 ttaggggatc gcggttcggg tgtccgcacc gcttaatacg tcgtcgtcca cgaactgacc
    19981 catttgctcg aacgccatcg caacgcccgt tttatggcgc atatggacaa ctttctccca
    20041 aactggcaaa gcatcaaaca acagcttaat gccttggagt tatttgcaca aatatataat
    20101 ttaacataat atatattatg cgaactatcg gaaacagttg tacgtgtccc tgtggtcttt
    20161 ccaagtagga aaattaaagt atggacaatg cggctgaatg tatagcccgg agcatccgcg
    20221 tatccgaagg tactgaacga tatgccctgc atccctttcg acgcttccat ccagccggta
    20281 ttgggaaata cgttgacctt caatacgata tacaggcagc cgacacacag gatatgccgc
    20341 tgctttttca tcatttcttc tgtcaaatcc ttggaacagt tgatttgaaa acacggcttt
    20401 acatacgtcc caattctctt tgcagtgctt ggatattttg ctgcctgtcc aatacattgc
    20461 tttgcaatgc gttgatttct tgatggttga tgttgccgcc ttttgccaga cgtgcttgtg
    20521 ataacatttt ttgggcttca accaatgctt tgcgttcgtt gctcaattct gtttcgagaa
    20581 tggagcgtct gctgttgttt gagggtgctt gttgcggcgg cggcgtattg gattttgccg
    20641 gcttggatac tgttttgacc ggggctttat atttgacagc ctgtccgccg tttgacggtg
    20701 atggtgtcgg ttcggatgtt tggggcggga tatagcgttc gctgctgtag ttgccgattg
    20761 ggggcaaatc ggttgagagg cagcttttgg acggcttggt ggtgtaaacg gtttctccgt
    20821 tgattgtgca ggtgtagatt ttggccgcat tcgcacccaa tgggcttgaa atcagggaaa
    20881 agttgattag gattaagggg agttttgatt tcataatgtg cttgatgctc ggataattat
    20941 ttgatttttt ggtatttttt gtaatgcttt cttggcgttt tacgggcttt tcttttcggt
    21001 tggttgcttt tgattttctt tcggggcttt ggcagcctgc ttgttctttt tcccggcagg
    21061 ttttttgttt ttctttttac tttctatgtg tattttggct tcttaactga gttttttaat
    21121 tttcaggcgg tatccgcctc cctgatggct gctgatttta ggtaaatccg catcggcgca
    21181 cgatcctgct ggggctgatg tatatacgct tttgccattt gagttgcaat ggtatacgga
    21241 ggcttgcgcc gccgcgccag aaagggacag gagacccaag gcggcaaaaa gtctgatgtt
    21301 tataccggta aaaggcggtg ataacgcccg aattataccg ttattggcag gcaaagataa
    21361 gcaccctgcc cgcgcttctt tatcgctcgg caaactgttt ctcgatttca gtttcaatcc
    21421 gttccaattc ttcttgacaa gccagccaaa cctcttcgat ttgggcaagt tgtgttttga
    21481 cttttgccag ctgggataag gtattttgca atttttcttt gtttgcttca aggtaagcgt
    21541 cttcttgtgc taaaaatgct tcacatgccg tctgaatttc ggaaagctgc gccatttctt
    21601 tttcggcacg gtctattttc tgctgtatcg gcttgccgcg tcgggctttt tcctgacgga
    21661 tttgcgcttc gatgcgcttg gtgtctttgc ggctttggct ttgtgcggat gctgctggtg
    21721 cggcggcgtt ttcctgtgcc aaacgccatt ggcggtaatc gttcaaatcg ccgtcgaagt
    21781 tcttcagacg gcctttgtcg atcaggagga agctgtcggt agtcgcttca agcaggctgc
    21841 ggtcgtgcga tacggcgatt aatgcgcctt ggaaactttg caacgcgagc gttaaggcgt
    21901 ggcgcatatc caagtccaaa tggttggtcg gttcgtcaag cagcaacagg ttcggctttt
    21961 gccagataat catggcaagg gcaaggcggg ctttttctcc gccggaaaaa ggttcggttt
    22021 tctgtaacgc catatcgcca acaaaattga agccgccgag gaaatttcgg atttcttgtt
    22081 cgcgtacttc gggagaaagc tgctgaatat gccaaacagg gttttggtcg gcgcggatgg
    22141 tatcgagttg gtgttgggca aaatagccga tattgagttt ttcggaacgg acgatgctgc
    22201 cggagagcaa atcgattttg cccgccaagg ctttgataaa tgtagattta ccgctgccgt
    22261 tgacacccaa taaaccatag cgcgcgccgc tttccagcga cagggtaatg tcgtgcaaaa
    22321 cagttttgcc ttcgtaaccc aaatctgcgt gttccagctt cagcaaagga ttgggcagat
    22381 gctcgggatt gtaaaactca aaggaaaact cgctgtccag atgcgcgggc gcaacgcgtt
    22441 ctagctttgc caaagccttc atgcggcttt gcgcttgaac ggctttggtg gctttggctt
    22501 tgaagcggtc gataaaggat tgcaaatgtt tgatttgcgc ctgctgtttg acataggcag
    22561 cttgttgttg cgcgagacgc tgcgcgcgtt cgttttggta aaaatcgtaa ttgccgccgt
    22621 attgcgtgag tttttgctgc gataattcaa tggtttgggt agttgccgcg ttgagaaaat
    22681 cgcggtcgtg ggaaatgatg atttgcgtgc agggtaaaga agcaagatgg ttttccagcc
    22741 acaagacggt ttccaaatcc aagtggttgg tcggttcgtc caaaagcagc aaatcggcgc
    22801 ggcaaatcag ggcttgggcg agattgaggc gcatacgcca gccgccggaa aaggatttga
    22861 cggggcggct gtgttcttct tgtgaaaaac ccagcccgtt caacaatttt gccgcgcggg
    22921 ccggcgcggt ataagcgtcg atttcttcca atttggcatg atattccgcc tgcttcatgc
    22981 cgtcattttg cgcttccgcc tgcctcaatg ccgtctgaaa agcctgcaac tcggcatcgc
    23041 cctgcaaaac gtaatccaaa gcggaaatat ctaaatcggg cgtttcttgg gaaacggaag
    23101 cgagccgcca gtttttcgga atcgagacat cgccgccgtc ctgagtgatt ttacccttga
    23161 ttaaggcaaa caggctcgat ttgcccgttc cgtttttgcc gatcaaaccg acgcgctgac
    23221 cgggattgac ggtagcgttg gctttgtcga gcaggacttt caaaccgcgt tgcagggtga
    23281 ggtttttgat ttcaatcata acggaaacat cgtcgggcgg aaaaagcccg tattttacct
    23341 gaaagtcagt gccgatgccg tctgaaatgg gaaatttacg gctgaagcca agcccaagcc
    23401 ctgcgccctt ccgagtgcag gaaaaccaat gtcctgaatg ccgaatcggt attcatgcat
    23461 tccacgctga ttccgattcg ggaaaaatcc gccatgattt tgggatggat aaactcctga
    23521 gccgcgcccg tcccgataat caatatttcc ggatagtcaa caggtttgat gtcggacaac
    23581 aggttttccg gagtcagatc ggacaaggtt cggcattgcg acaggcagac cgaatcctta
    23641 tgtacaagca cgggtttatg gaaactttgc cccgtcagcc ggattccgcc cgcaccgcat
    23701 tcatattccg caaactgtcc gtctatcgga ttttcttcaa acaacatttt tttaccccgt
    23761 cgccgcatca tctacaccga aaggggtgca aaatcaggcg aattcatgta ggattggcag
    23821 atttcatctg acccgcctgc cgatttcaga cggcatttga ttcaaagtgc ggcacaatta
    23881 tatcgacagc ggatattttc gtctttcaat atttacattt cagtcggctt acaaggagac
    23941 acaatgaagc cagtaaacat cggtctttta ggtttgggta cggtcggcgg cggtacggct
    24001 gccgtgttgc gggacaacgc ggaggaaatt tcccgtcgct tggggcgcga aatccgtatt
    24061 tctgccgtgt gcgatttgag tgaagaaaaa gcccgacaaa cctgcccgtc cgcagccttt
    24121 gtcaaagatc cgttcgaact ggtcgcacgt gaagacgtcg atgtcgtcgt cgaattgttc
    24181 ggcggtaccg gcattgccaa agatgcggtg ttgaaagcca ttgaaaacgg caaacacatc
    24241 gttaccgcca acaaaaaact gctcgccgaa tacggcaacg aaatcttccc gctggcggaa
    24301 aaacaaaacg tcatcgtcca atttgaagcg gcagtagcgg gcggtatccc aatcatcaaa
    24361 gccctgcgcg aaggtttggc ggcaaacagg attaaatcca tcgccggcat tattaacggc
    24421 accagcaact tcatcctctc cgaaatgcgc gaaaaaggca gcgcgtttgc cgatgtactg
    24481 aaagaagcgc aggcattggg ttatgccgaa gccgatccga ccttcgacat cgaaggcaac
    24541 gatgcgggcc ataaaatcac catcatgagc gcactggcat tcggcacgcc gatgaacttt
    24601 tccgcctgct acctcgaagg catcagcaaa ctcgacagcc gcgacatcaa atacgccgaa
    24661 gaacttggct atcgcatcaa actgttgggc attacccgca aaaccggcaa aggcatcgag
    24721 ctgcgcgtcc accctaccct gattcccgaa agccgcctct tggcaaacgt caacggcgtg
    24781 atgaacgccg tgcgcgtcaa cgccgatatg gttggcgaaa ccttatatta cggcgcgggc
    24841 gcgggcgcat tgccgaccgc ttccgccgtg gttgccgata tcatcgacat cgcccgcctg
    24901 gttgaagccg ataccgccca ccgcgtaccg catctggcgt tccaacccgc gcaagtccaa
    24961 gcgcaaacca tcctgcctat ggacgaaatt accagcagct actacctgcg cgtccaagcc
    25021 aaagacgaac cgggcacgct ggggcaaatc gccgcgctgt tggcacaaga aaacgtgtcc
    25081 atcgaagcac tgattcaaaa aggcgtgatt gatcagacca ctgccgaaat cgtgattctg
    25141 acccacagca cggtcgaaaa acacatcaag tcggcaatcg cagccatcga agcactggat
    25201 tgcgtggaaa aaccgattac catgatccgc atggaaagcc tgcatgactg agccgaaaca
    25261 cgaaatgccg acggaagagc aggttgccgc gcgcaaaaaa gcaaaagcca aaatccgcac
    25321 catccgcatt tgggcatggg tcattttggc gttgctcgct tcaaccgccc tgctctccca
    25381 atgcgcgatg tccaaaccgc aggcaaaaca gaaaattgtc gagtcttgcg tgaagaatat
    25441 tccgtttgcc gaaaaatggc aaaacgattt gcgggcccgc ggtttagatt caaacaatac
    25501 ccgccttacc gtcgactact gcaaatgtat gtgggagcag cctttggaca gattgagcga
    25561 gaaacagatt agttccttcg gcaaactcgg cgcacaagaa cagcttgacc tgctcggcgg
    25621 cgcaaatgcc tttgaaacgc gagacaagca gtgtgttgcc gatttgaaat cagaataatg
    25681 tggaccgata aaaaagccga ttctttaaag aatcggcttt tttcataaaa aacggcttac
    25741 agtgcgtctt tcaatgcttt acctgcacgg aatttaggcg ttttggcggc ggcaatggtc
    25801 agaggctcgc cggttttggg gttgcggcct tggcgttccg cacgttcgcc cacgtagaaa
    25861 gtaccgaaac cgaccaaagt aacggtgtcg ccttgtttca gggcggtggt tactgcattg
    25921 gtagtggcat ccaaagcttt ttgtgcggcg gctttggaaa tgtcggcttc ttgagcaatc
    25981 gcttcgatca attcagactt gttcacaatc agtcccttcc tgtcttaaaa aatgatgaaa
    26041 tgcccgaata ctcggggttt gtactgcttg agcaactttc gctttatagc aattctgaaa
    26101 ttgccgtgtc aagcaaaaaa tacggaatca ccctatttga caggctttca ggacgaaacc
    26161 gcatttttac aacacatttc ctgcgtttca atgtttggtt gccctgctgc ggggttttgg
    26221 ttttgaagcg gattccgcca ccgcttccgc accagaaggt tctgcccaag gctcaggctg
    26281 gctttccaaa cccagagcca atacctcgtc tatccatttg accggatgga tggtcaggcc
    26341 ggttttcacg ttttcaggga tttcttccaa gtctttgaag ttgtcttttg gaattaaaac
    26401 gtgtttgatg ccgccgcgaa gcgcagccaa cagtttttcc ttcagaccgc caatcggcag
    26461 gacttcgccg cgcagggtaa tttcgcccgt catcgctaca tcggcgcgta ccgggatttt
    26521 ggaaaatgca gacaccatcg ccaaagtcat ggcaatgccc gcactcggac cgtccttcgg
    26581 cgttgcacct tcgggaacat ggacatggat gtcttttttc tcgtaaaaat cagaagccaa
    26641 gcccactgat tccgcgcggg agcggacaac cgaccacgct gcagacacgg attccttcat
    26701 cacatcgccc aactggccgg tgcactgaat cacgccctta cccggcaatg ctgcggcttc
    26761 gacggtcagc aattcgccgc cgacttctgt ccacgccaga ccggttactt gtccgatacg
    26821 gttttcgctt tcggcaacgc cgtaatcgaa gcggcgcaca cccaaatagt cgtgcagatt
    26881 tttctcattt actttaaccg ctttaggttt ggctttgctg gttttcttgg tttcagacaa
    26941 cctcttctta tcttcgtcca aggtaatctg catcaccacc ttgcggcaga ttttggcaat
    27001 ctcgcggtcg agcgaacgca cgcccgcctc gcgggtgtag taacggataa tatcgcgcac
    27061 cgcgctttct tcgacgagca attcgccttc tttcacgccg ttgcgcttca tttgcttcgg
    27121 cacgaggtac tgcatcgcga tattgatttt ttcgtcttcg gtgtagccgg acagacggat
    27181 gatttccata cggtcgagca gcggggtcgg gatattcagg ctgtttgaag tcgcaataaa
    27241 catcacatcg ctcaaatcgt aatccacttc ggcataatga tcggcgaact tgttgttttg
    27301 ctcgggatca agcacttcga gcaacgcgct ggcgggatcg ccccggaagt cgctacccaa
    27361 tttgtcgatt tcgtcgagca ggaacaatgg gttttttacg ccggcttttg ccatattctg
    27421 caaaatctta ccgggcatgg agccgatata ggtgcggcgg tgtccccgga tttcgctttc
    27481 gtcgcgcacg ccgcccaaag ccatgcggac atatttccgc cccgttgctt tggcgatgga
    27541 ttcgcccaaa gaggttttgc ccacgcctgg agggccgacc aggcacagaa tcgggccttt
    27601 gagtttgtcc atacgttttt ggacggcgag gtattccaaa atccgttctt tgactttttc
    27661 caggccgtag tggtcggtat ccagcaccaa tccggctttg gcgatgtctt tgctgacgcg
    27721 ggattttttc ttccacggca gttcgagcag ggtgtcgatg tagttgcgta cgacggtgga
    27781 ttcggcagac atcggcggca tcattttgag ctttttcagt tcggacaggc atttttcttc
    27841 cgcttctttg gtcatacccg cctttttgat atctgcttcc aaggcatcca gttcgccgtt
    27901 ttcgtcttct tcgcccagtt ctttgtgtat cgctttaatc tgttcgttca gataatattc
    27961 gcgctgggat ttttccattt ggcgtttgac gcgtccgcgt atgcgttttt cggcctgcat
    28021 aatgtcgagt tcggattcca gctgtgccag caggaattcc atccgtttgc cgatttcggg
    28081 aatttccaaa atctgttggc gttgcgccag tttcaactgc aaatgcgctg cgaccgtatc
    28141 ggttagccgg ctgttttcgg caatgccgtt aatgctgccg ataatttcgg cggggatttt
    28201 tttattgagt ttggcgtatt gttcaaactg cgccaacagg gtgcggcgca cggcttcgag
    28261 gtcggtattg ccgcccgtgt cttcttccac gaccgcctct atatgggaaa cgaacagccc
    28321 gcccgtgtct tcaatggtca gaacacgtcc gcgatacagc ccttcgacca atacttttac
    28381 cgtgccgtcg ggtagtttca acacttgcag gacttgtgcg accgtaccgg tctgatacag
    28441 gtcggcggca accggttctt ctaccgccgc atcggtttgc gccaacagga aaaccggctc
    28501 ctcgcgggta atggcgtttt ccagtgcggc gatggatttc ggcctgccga caaacagcgg
    28561 cagaaccata tgcgggtaaa cgacgacatc ccgcaaagga agggttgcca aggcggcata
    28621 ttcctcaaaa tgcttttctt tttgtgtcat aggtactctc ttgtgtctga cagattgccg
    28681 attttcgcgt acattggggt tgaaggtatt atttcaagca tatgtggttt atttatggag
    28741 tttgatgcga tgccgtctga aacattccgg cttcagacgg catgggcttg gaaagacaag
    28801 gcgggaacaa aaaactgttc tgtgttgccg ctccttgctg taccatccgt atggtttgcg
    28861 gttctgccgc cctattttca aaacatgacg caggtattcc atgtcttctt ttatttttcc
    28921 cgataacggg ttcgaatggc gcaacgaaag tctgcaaaag ccgcccaaag gttggcatta
    28981 tgtccgcgaa agtggtgcaa acggcatttt gaaggctgcc tatcaaaata ttgcaactgt
    29041 ctggcagggc gatttccaca atgccaaaca ggtgctttcc gcaatgaaga agaaggtccg
    29101 caaacctgcc gccgtccgtt ccgatgcgga tatcgccgcc cttttccacg cccaccgtat
    29161 gaagcaggcg cagcagagcc gtattctgaa tatgcttgcc gttgaaatcc gccccggttt
    29221 tgtgttggac aacaaacgcg cgcccgatat acgcgccgcc ctgctcgacg tgtacggaga
    29281 ggcggacggc aaaccgtttt tcctgccgct caatctgctg ctggggttta tgggtgcgca
    29341 cgagtggcat aagaaagggg ttgccgttcc gcagctgggc ggcagcatac acgttccttt
    29401 cggcgtattc tcgccgttgc gcggcgaata cctcgacctg ctcgcccatg cgccgtcaac
    29461 gggttttcag acggcattcg atatcgggac gggctcgggc gtgcttgccg ccattttggc
    29521 gaaacagggc attccttccg tcatcggcac ggataccaat ccgaaagccg tcgcctgcgc
    29581 ccgtgccaat attgctcgtt tgggctttga aaaacaggtt gagatacggg aaaccgatct
    29641 gtttcccgaa gggtttgccg atctgattgt ctgcaatccg ccctggcttc ccgccaagcc
    29701 gacttccgcc gtcgaatccg cgttatacga ccccgaatct gcgatgctgg ctgcgttttt
    29761 gcgggatgcg ccgaaacatc tgaatcccga cggagaaatc cgcctgatca tttccgatct
    29821 tgccgaacat ctgcacctgc gtccgtccga ttttctggat aaggcatttg ctcaggcggg
    29881 tttgcgcgtt gcggatgttt tgaaaaccaa accggttcac aaaaaagctg ccgatccgaa
    29941 caatccgctg gcttttgccc gaaacaggga aactactttc ctgtaccgtt tgaaaaaggc
    30001 ataaggggcg gcggtgcgct tccggcggca ggaatctgaa cgcccgcgcc gccggcacgg
    30061 catcattcgg gcggattatc cttgtgaaaa tacccgctca agcatactgc ccaatgccgt
    30121 ctgacgcgtt ttgacagtgg cggcagcttg ccggaaggtt tcttctccgc cgaagaatac
    30181 gaacttctcc ctcgcgcggg taatggcggt atataacagc tccttactca atccggacaa
    30241 tgcatcgtcc ccttcgtccg aaggtgcggc ggaaggcggc agcagccaga cttcccgata
    30301 ttccgaacct tggcttttgt ggacggtcat ggcgaatgcg ggttcaaatt cgggcaggca
    30361 gcttaccgct acctttttaa atccgtccgc atcggcaaaa taggcggcaa ggctgccctg
    30421 ccgtccgaca tcttccataa tcagtccgat gtcgccgttg aacagttcaa gcgcgtagtc
    30481 gttctgcctg atcataatcg gctctccggc aaaatatgcc aaatgttccg gtatgttcat
    30541 tttgcggcgt acatggcggc aataggcttc gttgaagtct tccgcatcct gccgccaagc
    30601 tgccagaacc acgatatccg aaatgcccgc gtatgcggct tcgatattgc cgtcttttac
    30661 cgcctgccaa taggctttgt gtgcccggta caacctttcg actcgagcgt tcggactgca
    30721 ttccgaatgt tccagttcgt ccggaaaccg gtcaaacaat gcccacgccc cttcatcgcc
    30781 cgatacggcg gcacgggcaa ggcagccgat gccgctgttg tcgccgaagc ggtggctgaa
    30841 cgacagatgg gcggtgtttt gcgccaacac gggtggattt gcgctgacgc tgaaaccgtg
    30901 ttccggaagg aagccggcca gcctttggtg cgtttctccg tccaaaacgg ttttttgtga
    30961 caaaacggac agcaccgccc ctattccgac ggacgggagc tggttttcat cccccagcag
    31021 aatcacgcgc gcgccggttt tgaccgcttt taaaagttgc agcatcaatg ccgtatccag
    31081 catagaggct tcatcgataa ccaatacgtc aaacggcagc gggtaaacag ggttgaacgc
    31141 cgcctgcatt ttgggcgggc gcagcttcag cagtcggtgg acggtttgcc cttccagttt
    31201 gagcaaatgg cggcggacgg cctccagcgc gtcaaaaccg ttgattgcac ggtgcagcgc
    31261 gcgcgccata tgtgccgccg ctttgcccgt cggtgcggca agcgcgatgt tgggaagatt
    31321 ttcgtcttta ccgcaaatca gcgccagcag tttggcaacc gttgtcgttt tgcccgttcc
    31381 cggcccgccg gtaatcacca taaaagactg caacagtgcc aaggcggcgg catcgcgctg
    31441 cccttcgctg cccgtgcctt gaaaccattt tgcgaggttt tgcctcgcgc ctgccgcgtc
    31501 gggggcggac gtgtcggctg ccgccaagcg ttttatctcg gcagccaaat cgtattccaa
    31561 ctgccacatc ctgcccaaaa acagccttct gccttccaaa atcaaaggcg cggcggatgt
    31621 tccgacaacg ggtgcgagtg ccgacagcgc gtcagcctcg ccaccgctca aacggataaa
    31681 cgaatgaccg ttttgcaatg cctgaaacag gcgttcggtg cagtttgcaa gcacttcgtc
    31741 gccggaaccc gcatagtgtt ccaaaaaacg gattgccgcc cttgccgccg cttgggcaaa
    31801 ttcatctgtc tgccgttcca ttttattcct tatccaaatg ccgtctgaag gcgtggggct
    31861 tcagacggcg cggtgttgtt tcggctgttt aggcgtttgc gccctgtttg gggtgcaggc
    31921 tgccgtcttt catgaccatc acgcgctcga agcggccggc gagttcgtcg tcgtgcgtta
    31981 cgaccaccag ccccgttccc aattctgttt tcagttccag catcatatcc aaaacattcc
    32041 tggcgttcgc acgatcgaga ttgccggtcg gttcgtcggc aagcaggcat ttgggttggg
    32101 taaccaaggc gcgtgcaatg gcggcacgct gccgctcacc gcctgaaagt tcgcccgcgc
    32161 ggtgcgtcga acggtgtttc agtccgacct tttccagcat cgccattgcc gcctccgcag
    32221 cctcttcacg gctttttttg ccgatcagaa gcggcatcat cacattttcc agtgccgaaa
    32281 attcaggcag aagatggtgg aactggtaca cgaaaccgag atggcggtta cgtaaatcgc
    32341 ccaaacgccg ctggtttaag gtacgcaaat cctcgcccat cagcagcacc ctgccttcag
    32401 acggcatatc cagcccgcct agaatatgca gcagcgtcga ttttccgctg cccgaagaac
    32461 cgatgatgcc ggtgctttcc cccgcgcgga tttccaaatc caagccgtgc agcaccagaa
    32521 cgtccaaacc gccgtcccga tagcgtttgc ccacgccttc gcatttcaaa atcaactcac
    32581 tcataacgca aagcctccgc cggttgggtt tttgacgcgc gccggctcgg gtagagcgtc
    32641 gccacgaaag acagccccaa ggaaatgcag gcaatcaggg caacgtcgcc catatcgaca
    32701 tcgctgggca ggtagtcgat aaaataaacc tgcgaattga tgaggtggac accgagcagg
    32761 ttttcaaaaa acgccacgac cctgccgacg ttccaaccca aaagcacgcc gcagaccaca
    32821 cccgccaacg tgccgaaaaa gcctgaaaac gcgccctgca ccataaaaat cttcatcacg
    32881 ccgccagggg aaagacccaa agtccgtaaa atcgcaatgt ccgcctgctt ttccgtaacc
    32941 gccatcacca aggaagagac aaggttgaac gccgccacgg cgataatcag cgtcaggatg
    33001 atgaacatca tccgtttttc caactcgacc gcctcaaaat agctgcggtt gctgaacgtc
    33061 caatcgcgta cccaaaccgt atccctttgc gcctccggaa tcagctttgc cgtcaaggcg
    33121 ggggcgtttt gcgggtcggc gagtttcagc cgcagccccg caacttcctt atccaagcgg
    33181 tacagtacgc gcgcgtcttg gatatgcgtc atcgccaatg agttgtcaac ctcgtaaaca
    33241 cccgttttaa ccagaccgac cacggtaaac tgtttcaacc tcggtacgac tccggcgggc
    33301 gtaacattgc cctccggcgt gatgacggta actttattgc cgacttccgc ccccaaagcc
    33361 tccgccaagc cgacaccgag gataatgtca aactcgcccg gaatcaggtc ttcaaatttg
    33421 cctgccggca ttttgtcgcc gtattccacc actttgcgct cttcagacgg caaaatgccg
    33481 cgtatctgca cgcccctgat ttcgcccgca ttggccagca atgcctgatt ggaaacatag
    33541 ggcgcggcag ccaaaatacc tttgcggttt tcggtaaacc gaagcaggtt gcgccaatcc
    33601 gtatccgtat tgtcgatata gccgatttcg gcgtgcggcg cgacattcag gagctgcccg
    33661 cgtatttctt tctgaaagcc gttcataacc gacaagacga caatcagcgc ggttacgccc
    33721 aaggcgattc cggcaatcga aaccatcgtg ataaacgaca taaagccgtt gcgctttttc
    33781 gccctgagat acctcaagcc tatccaagcc tctagagaaa acataacgct accttaaaaa
    33841 tgtctgcaaa cgtgccgccc ccggacggcg gtttgggggc gacggcaaaa gtttattgta
    33901 ccgtaaaacc caggcagcgt ccgaacgccc gagtgcgggg gacggggcgg cagagcgggc
    33961 ggaaaacttg cacaacgcgt caaactgccc tatcctttcc ttcagaaaaa ccgtttcttg
    34021 aggaaaacaa tgaatatccg aactgctttt gctttgtgcg ccatcgcctt atccgccgct
    34081 gcggctgcct acgccaaaga aatcaaaatc gatgccaaca acacgcctta ttccgaagcc
    34141 gacgcgcaaa agctggcggc aacggcagtc ggtatgggtg ttaaggaacc tatcagcctg
    34201 aacggcggca gcggcagcat taccgtgtcc ggcagcagcg cgacgcagtg cgtgttcaaa
    34261 gtcggcaacg gaggcgcgtt gcagattcaa gggctaaact gcaagtaaac cgcccggaaa
    34321 aatgccgtct gaaggcttca gacggcattt tgcattggcg gcgttatgcc ccgccttctt
    34381 taatcaggcg gcgttcgtac accgcctgcg ccagcgttcc cgcatcgaca tattccaatt
    34441 cgccgcccaa gggaatgccc tgcgacagcc tgctgacttt gtaaggcagg tttttaaaaa
    34501 actcggacag gacatacgcc gtcgcattgc cttctgcggt aaaagcggtt gcaataatga
    34561 tttcttcgac ttccccgccg cccagccgtt gcgccagcct gtccaatgcg atggcggata
    34621 cgtccattcc caatgccgta ttgatttgcc ccatcaggac gaaatacagc ccgtcgtggc
    34681 agtttgccgc ttccatattc gacacgtcgg caggcatatg caccaccatc agccgtcgcc
    34741 cgtcgcgtgt ttcatcggca caaatatcgc acaatccgcc ttcgcaaaac gtgttgcacc
    34801 tcgcgcaatg atcgacctgc ttcaatgccg tctgcaaggc atccaccagt ttttcagcct
    34861 ctttgcgctt gtgttggagc aaatgatacg ctatccgctg tgccgatttc ggcccgacgt
    34921 tgggtaaaac cttcagcgcg tcgatcaatc cttggaaggc atcttgtttt ttgtggctca
    34981 tcatattccg ccgtatggga aaacggccgg aatattccga ccgttatttt gtcaacaaaa
    35041 gtgtcaatta ctgaccgtcg ccgttgtcga ccgattgcgc tcctttggtc tgtttgattt
    35101 tgccgttgaa ataacgtatc aacaagtcga aagtattggc agactgctgt tgcgccaaag
    35161 cctgttttgc aagcggaagc tgtgcggcga tatcatccgg cggggttaca gcctgtactt
    35221 cgacaatcac gggtgccggc agaccgatca gcctgacgta ggcgggtttg ccgtttgccg
    35281 gttttgcttt cagcagttcc gcataagcct cgggcggcat ggactgcctt gcctgctgtg
    35341 cgcccaaaac ggacacttcc gaccatttca cgtcaacagc cttgccgccg ttcagttggg
    35401 taagcacgtc ttttgccttg ttttcggcaa gtttggcggc ttcggtacgg atataagcct
    35461 gacgtaccgc gtctttggct tcggcaaacg gcagggtttt ctcttcgcgg acttctttgg
    35521 cgcggacgac ccacgcggtt tcgctgttga tggtcagcac ttcggaattg tgttttttct
    35581 tcaatacgtc gtcgctgaat acggcattga tcaggttttc gggcataccg gacatttgcg
    35641 catcctgcct gctcagccaa gtttcttggg tttcgacttt caaaccgctg tttttggcgg
    35701 cttcggcaag cgaggaagga tggttgaacg catcatcgcc cagcttttct tttgccttat
    35761 tgaagtcggc aaccgccttt ttcattttca attcgttttc gacggcggct ttttcctgct
    35821 cgaaagaagg tttggcttca tttgccggca aacgcgccac gcgctcttca aacgcatttt
    35881 tcacttctgt ttcgctgacg gtctgtttgt ctgcaaagtc tttcagattc aaggcgacat
    35941 attccaattt gaccgctttg ggaagcaggt agtctttttt gtttgcgtta taaaacttct
    36001 gcaaatcggc ttcagacact ttgacttggg cgatgaattc gtcggggttg aaagtgtgcg
    36061 aacggatggt gcggttgacc tgcgtcagcc tgatcagctg ttccgcctgc gcgtcgccga
    36121 ccaatacgcc gttttggacg aggtttacca aattctgcaa ggcaaactga tcgcggattt
    36181 cttcgacaaa ctggtcttca gacatatgac gttgggaaag gtagcggttt aaaagcgcgt
    36241 ggtcgaattt gccgtttgcg tcgtggaaat tgggatcgtc cacgataatc tgcttgattt
    36301 gttcggaaga aaccgaaatg cccatcagct tcgcgccctg tttcaggtag gcgcgttgta
    36361 gcagggattg gaacaccgcg tcgcgcgaag ggccgccgcc gtccgcctgt tcgttctgta
    36421 tggcgttgtt gatggagtgg tcgctgattt tttcgtcgcc cacttggacg atgtagtcgg
    36481 cacccggatg ggataccgtg ctgaccccga agccgacgaa ggttaatgca atcaggccca
    36541 aaaggacttg ggcgggcgtt ctgtattttt cgatggaatg gaacatattt taaatcggga
    36601 tatagaatgg gaaattcaag tcgggtattg taacggtttt tatccctgtc tgcacggggc
    36661 ttgccggttg aagatgccgt cgtaggtttc ttcgctgaag ctggcgtaaa ccttgccgcc
    36721 gcactccaat acgggacgct tgatcaggct cggcatttcg gacatcagtt tgacggcctc
    36781 cgccgtcgag gacagcactt tttgctgtgt ttcggcatcg agtttgcgcc agcttgtccc
    36841 gcgtttgttg agcagggttg ccaaaggcac ttgttccagc cacgagcaga tttccgcttc
    36901 agacggcctc tgttttttaa aatcccgaaa ttcaaactcc aagccgtatc cggcaagccg
    36961 gtttttggct tttttgaccg tatcgcaatt tgggatgccg tgaaggatta tcatttggaa
    37021 accttttgtc tgaaataata aaacggatat tttactataa gtgtctgaaa atttgcccgt
    37081 ctgtttcaga cggcggggcg gttatgttac aatccgaaaa ttcgaaaaat ttaatctctt
    37141 gttcaataaa ggctttacca atcatgattt ctaccaacgg catcactatg cagttcggcg
    37201 cgaagccgct gtttgaaaac gtatccgtta aattcggcga aggcaaccgc tacggtttga
    37261 tcggcgcgaa cggctcaggc aaatccacct tcatgaaaat cctcggcggc gatttggaac
    37321 agacagccgg cgaagtggcg attgaaaacg gcgtgcgttt gggtaaattg cgccaagacc
    37381 agtttgccta cgaagatatg cgcgtgctgg acgtggtaat gatgggtcac accgaaatgt
    37441 gggcggcgat gaccgaacgt gatgcgattt acgccaatcc cgaagccacc gaagacgact
    37501 acatgaaagc cgcagaactg gaagccaagt tcgccgaata cgacggctac accgccgaag
    37561 cgcgtgccgc cgagttgttg agcggcgtgg gcatttccga agatttgcac aatgcaacca
    37621 tggcagaagt tgccccgggc ttcaaactgc gcgtattgct ggcgcaagcc ctgttctcca
    37681 agccggatgt attgctcttg gacgaaccga ccaataactt ggacattaat accatccgtt
    37741 ggttggaagg cgtgttgaac cagtacgact ccacgatgat tatcatctcg cacgaccgcc
    37801 acttcttgaa cgaagtctgc acgcatatgg cagatttgga ttacaacacc atcaccatct
    37861 atccgggcaa ctacgacgac tatatgctcg cctccgccca atcgcgcgaa cgcgccctga
    37921 aagacaatgc caaggcgaag gaaaaattgc aggaattgca agaatttgtg gcgcggttca
    37981 gtgccaacaa atccaaagcc cgtcaggcaa ccagccgtct gaaacaggca gacaaaatca
    38041 aatcggagat ggtcgaggtc aaaccctcta cccgtcaaaa cccgtatatc cgttttgaag
    38101 ccgatgaaaa agccaagctg caccgtcagg ctgtggaagt tgaaaaactg gcgaaacgct
    38161 ttgaaaccca gttgtttaaa aacctgaact ttatccttga agcgggacaa cgcctcgcca
    38221 tcatcggccc gaacggcgcg ggcaaatcca ccctgctgaa actcttggcc ggcgcgtaca
    38281 accccgaata ttcagacggt ctgctgccgg acgaaggcag catcaaatgg gcggaaaaag
    38341 ccagcgtcgg ctactatccg caagatcacg aaaacgactt cgatgtcgat atggatttga
    38401 gcgaatggat gcgccaatgg gggcaggacg gcgacgacga acaagtcatc cgcggcactt
    38461 tggggcgttt gctcttcggc agtaacgatg tcgtgaaaaa agtgaaggtt ctctccggtg
    38521 gtgaaaaagg ccgtatgctt tacggcaaac tgttgctgtt gaaacccaat gtcttagtca
    38581 tggatgaacc gaccaaccac atggacatgg aaagcatcga atccttgaac atggcgctgg
    38641 aaaaatacaa cggcacgctg attttcgtct cccacgaccg tcagttcgtt tcttccctgg
    38701 ctacccaaat cattgaattg gacggcaaag gcggatatga acactacttg ggcgattacg
    38761 aaagctatct cgagaaaaaa ggcgtagcat aaccgccggt tggaacaatg ccgtctgaag
    38821 cggcttcaga cggcattgtt gataacttta aaataggaag catatgcaga cttatctcgt
    38881 cggcggtgcc gtccgcgatt atcttttggg cttgcccgtc aaagaccgcg attgggtggt
    38941 cgtcggcgca gacgcacaaa ccatgctggc gcaaggcttc cagccagtcg gcaaagactt
    39001 tcccgtgttt ctccatcccg aaacacacga agaatacgcc ctcgcccgca ccgagcgcaa
    39061 aaccgccaaa ggttacgtcg gtttcagttt ccacgccgac aaagacgtta cgctggagca
    39121 ggatttgatg cgccgcgacc tgaccatcaa cgcgatggcg caagatgcgg acggcaagat
    39181 tatcgaccct ttcggcggac aacgggattt ggcggcaggc attttgcgcc acgtttcccc
    39241 agcctttgcc gaagaccccg tccgcatcct gcgtactgcc cgctttgccg cgcgttacaa
    39301 gtttgaaatc gccgaagaaa ccataaagct gatgcggcag atggtggaaa acggcgaagc
    39361 ggacgcattg gttgccgaac gcgtctggca ggagtttgcg aaaggtttga tggaaaaaaa
    39421 tccgcgcaaa atgattgaag tgttgcgcga atgcggcgcg ctcaaagtct tgctgcccga
    39481 agtcaatgcc ctcttcggcg tgccgcaacg cgccgactac catcccgaaa tcgacagcgg
    39541 catccatacc ctgatgacgc tgcaacgcgc cgccgatatg ggtttgagcc tgcccgaacg
    39601 ctatgccgcc ctgctgcacg acttgggcaa agccaaaaca ccgtccgaca tcctgccgcg
    39661 ccaccacgga cacgacctcg ccggtgtcga acccgtgcgc aaagtcaatc aaaggctgcg
    39721 tgcgccgaaa cattgtgccg agcttgccga attggtttgc cgttggcaca ttattttcca
    39781 ccaagtcgga cagcttaaaa gccaaaccat tctgaacgta ttgaaaaaaa ccgacgcttt
    39841 cagacgaccc gaacgctttc agacggcatt gaacgtctgc attgccgaca cgcaaggccg
    39901 tctgaaccgc gaacacacgc cctacccgca acgcgcgcac tggctcgcct tactcgaagc
    39961 cgccaatcag gcggattcgg gcaaaatcgc cgccgaatgc cgctcgcagg gaaaagcgca
    40021 ccttatcgcc gaacaaatcg accgggcgcg gctggcacaa atcgccccat tgcaaaaagc
    40081 gtttcgagcg gcgcaagaca aaacagaaaa acattaaaac gtccaatgca gccactttta
    40141 tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca gatagtacgg
    40201 caaggcgagg caacgctgta ctggtttttg ttaatccact ataaagtttt gaggacgata
    40261 cccaatccaa gctttgcaac agccgccgcc atatccgcta taattcacgc ttcagccatt
    40321 ccgcccccga cataaaatca tgaccctgaa aaccgattta ttgcctaaaa tcaacaacga
    40381 agattatcaa cgcctcatcc tcaaacacag tgcggaattt agcggtggcg aaatccgcct
    40441 attgaacgaa atcctcgaaa aattcaattt cgacgttgtt caggcgcagg cgttggcgca
    40501 ggcggtgatg cagcaagtcc gcttcgaccc caacgcctat cacatcgaca gcgacgacga
    40561 agacaccaca ggcatctgcc cacactgcat caacccgcct atgccgcccc tgcgcgacta
    40621 tctcgtttgg cgcgaaacgc gcgggtaaaa cgcttttgac cgttatcttt tcaatgccgt
    40681 ctgaaacgcc gccgaccgtt cagacggcat acccgacaaa gggaacacta tgctgcaaac
    40741 cgacaacctg actgccgctc aaccgcaacg cattgttgcc gcccaaaccg cctccgcaca
    40801 ggaagaactg ctcgaacgcg ccctccgccc caaaacgctg gacgactata tcgggcagca
    40861 taaagccaaa gaacagctcg ccattttcat ccaagccgcc aaaaagcgcg gcgaagcact
    40921 cgaccacgtc ttgcttttcg gtccgcccgg gctgggtaaa accacactgg cgcacatcat
    40981 cgccaaagaa ttgggcgtaa atttgcgcca aaccagcggc cccgtcctcg aacgcgccgg
    41041 cgacctcgcc gcccttttga ccaaccttga tccgcacgac gtattgttta tcgacgaaat
    41101 ccaccgcctc agccctgttg tcgaagaaat cctctacccc gcgcttgaag actaccggct
    41161 cgacattatg ataggcgaag ggcccgccgc ccgttccgtt aaaatcgacc tgccgccctt
    41221 cacgcttgtc ggcgcgacca cccgcgccgg tatgctgacc aatccgttgc gcgaccgctt
    41281 cggcatcgtc tcccgcctcg agttttacga aaaccgcgac cttgccacca tcgtcagccg
    41341 ttcggcacaa ctcctgcaac tcgatatgtc cgaagaaggc gcggaagaaa tcgccaaacg
    41401 cagccgcggt acgccgcgca tcgccaaccg cctgttgcgc cgcgtgcgcg atttcgccga
    41461 cgtgaaaaac aacggcacaa tcgacggcgg catcgccgat gccgctttaa gtatgctgga
    41521 cgtggacgtg caggggctgg acgtgatgga caggaaattc ctcgaagccg ttttgcacaa
    41581 attcggcggc ggcccggtcg gtttggacaa tgttgccgct gccatcggcg aatcaacaga
    41641 caccatcgaa gacgttatcg aaccctatct catccaacaa ggcttcctgc aacgcacccc
    41701 gcgcggcagg atggcgaccg aacgcgccta cctgcatttc gggctgcccg tcgaaaaata
    41761 acgcaatgcc gtctgaaaca gagctaattt tcagacggca tttctatttc aatcattggc
    41821 gcaaggttca gcctgccgct tttttccagt tccgccctca tcgcatcaat caccgcctta
    41881 tagtctggtt tgccgaaaat cgcagaaccg gcaacaaagg tatccgcgcc ggcttgggcg
    41941 gcggcggcga tattgtcggt tttgatgccg ccgtccactt caatggcaat acgtctgccg
    42001 cttttctctt catagcggtc aagcatggca cgaacctgtc tgattttttc caaagtatac
    42061 ggaatgaagc tctgaccgcc gaacccgggg tttaccgaca tcagcaaaac catatccagc
    42121 ctgtccaata cgttttccaa cagatatacg ggcgttgccg gattcaacac cagccccgcc
    42181 tgacagccca tgtcccgaat caggctcaag ctgcggtcaa tatggcggct cgcttcggga
    42241 tggaacgtga tgattgatgc tcctgctttg gcaaacgact gaatcaggtc atccacgggt
    42301 tcgaccatca gatgcacatc gatcggcacg cttgcataag gcttcaacgc cgcgcaaacc
    42361 ataggcccga aggtcaggtt cggcacataa tggttgtcca tcacgtcaaa atggatcaga
    42421 tctgcacctg ccgcaatgac gctttccacc tcttcgccaa gccgtgcaaa atctgccgat
    42481 aaaatgctgg gtgcgatacg gtaagtagtc atgttttttt ccttcaatat c
//
LOCUS       DAWFLT010000008        42372 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWFLT010000008 DAWFLT010000000
VERSION     DAWFLT010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 42372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42372
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.008"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            40..368
                     /locus_tag="WGR74_000381"
                     /pseudo
     CDS             40..368
                     /locus_tag="WGR74_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213615.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            380..646
                     /locus_tag="WGR74_000382"
     CDS             380..646
                     /locus_tag="WGR74_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904872.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADSTNIAAALERIENKLEKVNEKLDRKADKS"
     gene            643..840
                     /locus_tag="WGR74_000383"
     CDS             643..840
                     /locus_tag="WGR74_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904873.1"
                     /translation="MSDPILEVLARIEAKQDDMLANQARMDEELQQIKKDCKKSAAVY
                     GGLGGVIVTTGWELLRAKFGG"
     gene            842..1348
                     /locus_tag="WGR74_000384"
     CDS             842..1348
                     /locus_tag="WGR74_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ0904874.1"
                     /translation="MAHPKEIREKLRRLYVSDGQTLEIAAMMCEIPIATARSWKRAAK
                     ESSDDWDKVRAAYTLAGGGIEDLSRSLLAGFLVQYQSTMTMLQDTSIEGLMPSERAKL
                     LTSLSDAFTKTVAANAKVMPETSKLATAIEVLELFGEVVRERYPQHLQAYVELVEPLG
                     VEIEKKYR"
     gene            1369..2991
                     /gene="terL"
                     /locus_tag="WGR74_000385"
     CDS             1369..2991
                     /gene="terL"
                     /locus_tag="WGR74_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ0904875.1"
                     /translation="MKSKEFLKSLAEYAAQLRQIIEAEVDGFDASPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTVLDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWHGIKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYLANKDEMERGAQTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRETG
                     KLYVVETQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            2991..4559
                     /locus_tag="WGR74_000386"
     CDS             2991..4559
                     /locus_tag="WGR74_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ0904876.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSLQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     SGTMATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTIASQIIGPFLQINYPNADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPINRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            4546..5871
                     /locus_tag="WGR74_000387"
     CDS             4546..5871
                     /locus_tag="WGR74_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEZ0904877.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLSNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTSDNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLNIDGKPDKEQKIKIRNALSRQLKFAAGVLSKEMQELAGM
                     TRATVWLSDDTLVKQVDSREGQSFGTDLYAMLPDLIHEPEYIFYDKSRGKESFVLLGK
                     PLEGNEKYRLMAVLKYLPQYDSIFLDSFRRADEKEIVKSKKRYEMKKGY"
     gene            5999..6415
                     /locus_tag="WGR74_000388"
     CDS             5999..6415
                     /locus_tag="WGR74_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ0904878.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            6646..7656
                     /locus_tag="WGR74_000389"
     CDS             6646..7656
                     /locus_tag="WGR74_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904879.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFGLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTSGKLLPAQKEWAEGVLKQPGGL
                     AFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAAKMLGMSGEEFVKIKESE
                     GK"
     gene            7656..8564
                     /locus_tag="WGR74_000390"
     CDS             7656..8564
                     /locus_tag="WGR74_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HEZ0904880.1"
                     /translation="MDKSAILTAITAAFRKEFQTGLESVKPDYTAVAMTIPSNTATNT
                     YAWLGKFPQMREWVGSRQIEKMNKQAMSLDNKKFEATVGVARTDIEDDQVGMYRPMMA
                     AMGESAAALPDTLVWGLLKKGKTTVGYDGQYFFDTDHPVYEKSDGTGQNTPHSNLTTG
                     TDNDAPTFYVVDDTKTLKPLIFQNRTDSEFETKFDPSKSDRVFMEDEYLYGSRRRCNA
                     GFGLWQLMHMAEKTALTRENLAAIIVKMQKIKADGGYVLNVKPSLLVVPPELEDKARE
                     LLEADKINGTTNTFKGRLKLHVCVHL"
     gene            8629..9027
                     /locus_tag="WGR74_000391"
     CDS             8629..9027
                     /locus_tag="WGR74_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904881.1"
                     /translation="MAKTNNKPETAETAAPSFEDIKAELDAVQAELAAARNDVEMLTT
                     ALEKAEDDKKALSAELAELKVQHTQRAADALADSRDVMLVSTGADGKEFWRGGLLFDG
                     GWREVKRAEVGEAVWKAICAEPMLQRKAVE"
     gene            9027..9449
                     /locus_tag="WGR74_000392"
     CDS             9027..9449
                     /locus_tag="WGR74_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HEZ0904882.1"
                     /translation="MAYATVEDMVARFGELEVLQLTDRNNEGLIDREVAQTALVDATA
                     EIDAYLGRFRRPFEDLPPILVRLCCDIARYRLTAAQGVLITDEIRNRYKIDVLDLLRA
                     MAKGEVQLGVDDSGEEVAAGEDGIVFVNGKNKVFGRDH"
     gene            9439..10089
                     /locus_tag="WGR74_000393"
     CDS             9439..10089
                     /locus_tag="WGR74_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ0904883.1"
                     /translation="MITDIEQAITDRLKRGLGRMVRTVKSYNGEADDLAGQIHTLPAV
                     WVTYGGSKVEPASTGGVCGRYQDTAEFVVMVAARNLRNEQAQRQGGIDSREIGSNDLI
                     RAVRRLLDGQRLGFADSRGLVPKAVRAIANHVLVQNAAVSIYAVEYAIRFNTCGLEND
                     RYPERTDNPDDPNHIFTKYQGTLSEPWPDFEGLDGKIYDPQSADEIPVNLTLKDKQ"
     gene            10086..10283
                     /locus_tag="WGR74_000394"
     CDS             10086..10283
                     /locus_tag="WGR74_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ0904884.1"
                     /translation="MSKIKVTAADGLRVPTEHNPHEYIGQEPVEVDGNSLYYRRMIDD
                     GDLVVVEDAAPNTKTRNTKGE"
     gene            10286..11695
                     /locus_tag="WGR74_000395"
     CDS             10286..11695
                     /locus_tag="WGR74_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEZ0904885.1"
                     /translation="MPHIDFDTIPGSIRVPGQYIEFNTRNAVQGLPQNPQKVLMVAPM
                     LTAGIQPALEPVQLFSDAEAADLFGQGSLAHLMVRQAFANNPYLDLTVIGIADHSAGV
                     QATATVTLSGTATAPGVVEITIGGKQVSTAVNTGETAATVAGRLKTAITAADVTVTAS
                     GSGAAVTLTAKHKGEIGNESGLTVSTGNTGLTYQANVFTGGAKNADIATALSKVAGKH
                     YHIICSPFSDDANAKALSNHITNVFNAIEQRGCIGVLGMSAALSTATTATGKINDGRI
                     TCAWYKGAVEPNGIIAAGYAAVLAFEEDPAKPLNTLEIKGLAVTPDAQWPWFAECNNA
                     LYNGLTPLTAVNNRVQIMRAVSTYTKSANNTDDPALLDITTIRTLDYVRRSVKERIAL
                     RFPRDKLSDRLLPKVKSEILDVLIKLDQAEIIENAEANKGKLVVARAQNDPNRVNAII
                     PADVVNGLHVFAGRIDLIL"
     gene            11760..12137
                     /locus_tag="WGR74_000396"
     CDS             11760..12137
                     /locus_tag="WGR74_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0904886.1"
                     /translation="MSDATYTGAVIMEVNGRDIEIVSIKPQTTTGRKPVKTMNRNGRV
                     NGYCDGVTEHKLSVTAAIPIDGTEIDWDNITKAKITIYPINDEGRRTSYLDCFTVDTS
                     EQYEVDNEARIDIEMIALHKIKE"
     gene            12141..12506
                     /locus_tag="WGR74_000397"
     CDS             12141..12506
                     /locus_tag="WGR74_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904887.1"
                     /translation="MITVKLTHGLTYNGKVVSELRLKPLTVGGELAAFALIDDLPELP
                     ENATKAELLQRDVLETLTYWSQQIEAQGIPSDILTAQWLMENLSTEDYHTVMAAQEDL
                     CLKPSAATASPDAPSAAEQ"
     gene            12446..12715
                     /locus_tag="WGR74_000398"
     CDS             12446..12715
                     /locus_tag="WGR74_000398"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904888.1"
                     /translation="MPKTVRRYGEPRRAVGGGAVKRSYLTAHKSYRQAVILMAKAGIG
                     ADAVRDMCHAELSAWFEDILSSLGIKTPKEEGVIVSKRLRKPEGK"
     gene            complement(12759..13341)
                     /locus_tag="WGR74_000399"
                     /pseudo
     CDS             complement(12759..13341)
                     /locus_tag="WGR74_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249360.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13496..15667
                     /locus_tag="WGR74_000400"
     CDS             13496..15667
                     /locus_tag="WGR74_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein F"
                     /protein_id="HEZ0904889.1"
                     /translation="MANGNMKLSLVLTARDDGARRLLADTQRQLDRTAKSRAQLERQS
                     HTYALTGIRSEKQIQREIMLTQAAFNRLARSGKASQNDLARAAVATRNRIRELNAELK
                     QGTGFADKMGKIGRFGAAAVAGGAAAYTVLKPAMDNRKQLDENINRVSRQAFIEDNSK
                     SAAWIATEGAQQIKDLALELVEKNGGTHDKALDLISGMMTTSLNFAQTKNEAQAAYAF
                     ALASEGSGEDTAKLIKTLKDGGMSGKDLQLGLEHVLQSGLDGTFEVRDMVRELPSLLP
                     AAQQAGMNGVGGLDYLLSLLQSAANKSGSPAEAATNVQNLLSKTLSPYTIGRLKKMAN
                     PNDPKKGVDWIGSVVQGKQNGENAVQVLSRLADAMLVKDKQYQDYKKRAAAGDKTAAE
                     QANMLKGALLAQLLPDLQAKQGLLAATDMTQIREYMASLAGVTLDNGKIAKNNEARML
                     SAAAQQEQQESLAMLRESLTGTLVDMETSFKKLAAEYPNATLALQALTTAATAASAAM
                     LLTAGGGKGAGFLKDVGSKALVWGKASAGGVAAGATAAGGKLLSWGKSAGSGLMNNPA
                     LVKRAGLLGMLLYSESLGDGTLPKGLRGTKTTPEMINRLKNNGIRFEPAPKREQARGG
                     VPQYLAAPSAQPTDKMLSPLFSTQTAAYQAAIQQQTAAYQAALAQDTAAVTTGLAQVQ
                     SAMASASQTINTNVSLNIDGRVIANEVSRYQVAMFGRGAGQ"
     gene            15667..16998
                     /locus_tag="WGR74_000401"
     CDS             15667..16998
                     /locus_tag="WGR74_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ0904890.1"
                     /translation="MSGWHTLLQDASYKGVGFDIEVVDESNGKALAEHARPFVQGIDL
                     EDMGMTGRQVQINAVFWGKGYAGRLKKLLDALEQPGGGVLVHPVWGRMHNMIAASWSY
                     RHEADYVDYAGIDITFREAAEAQEIFVFENAFLVELEALIADIDTYREAAIGFVDAVL
                     AVDAGVSALWGSALGIWSAASGTFGAVRRLFDLDKIAFPDRGGYSAAAFKNGSAKLFA
                     DISVMVDTGIRREAGLADNAMHHAGWSPRQRFDGAAAVADRAAAIPDNLLTGRFSDGL
                     QNRLNRLTAKQVQPVAQAVRLLSTSSLLSVATALIEAHGEEMTAPDLIEVNRAMRRRM
                     QAEIAALRAVQTAAAESGGLTANAVYTEAYQTAESLRAAAGRLNALVAAVINQKPPLI
                     VRQAPIDGTIHQIAHEFYGDIARAAELVRLNPHIHHPAFIKRGTLVNSYAK"
     gene            16988..18133
                     /locus_tag="WGR74_000402"
     CDS             16988..18133
                     /locus_tag="WGR74_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0904891.1"
                     /translation="MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGR
                     LGPEAAIPDLSGESCEVVIDGQIVMTGIIGSQRHGKSKGGRELSLSGRDLAGFLVDCS
                     APQLNVKGMTVLDAAKKLAAPWPQIKAVVLKAENNPALDKIDIEPGETVWQALTHIAN
                     SVGLHPWLEPDGTLVVGGADYSSPPVATLCWSRTDSRRNIERMDIEWDTDNRFSEVTF
                     LAQSHGRSGDSAKHDLKWVYKDPTMTLHRPKTVVVSDADNLAALQKQAKKQLADWRLE
                     GLTLTITVGGHKTRDGVLWQPGQRVHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELR
                     LKEDGIWTPDAYPKKAEAARKRKGKRKGVSHKGKKGGKKQAETAVFE"
     gene            18130..18363
                     /locus_tag="WGR74_000403"
     CDS             18130..18363
                     /locus_tag="WGR74_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HEZ0904892.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRAAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPTARRRQPLRL"
     gene            18314..18832
                     /locus_tag="WGR74_000404"
     CDS             18314..18832
                     /locus_tag="WGR74_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ0904893.1"
                     /translation="MPAIRRPPAGGNRYDYKNWALSVDGVTSAYVYPLRRGLGTVDIA
                     ITSADGVPSEETVRRVQAYIDEMRPVTAKNALVLKPTVTAVPVTVQVKLDGIDLDDAK
                     RRIRTALKEYFDTLIPGDGLTVSQIEAAISNVDGVIDRRLTAPTANRAADTVNRIEWF
                     KAGAINVTEMPS"
     gene            18829..19390
                     /locus_tag="WGR74_000405"
                     /pseudo
     CDS             18829..19390
                     /locus_tag="WGR74_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
     gene            19401..21374
                     /locus_tag="WGR74_000406"
     CDS             19401..21374
                     /locus_tag="WGR74_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ0904894.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGNQEIGGQKTFTAATQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHSANNRISYNNHDIFHKANKPRFAEDIEGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAGGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVRNPAGYLKANGTTF
                     AQNTFPDLYRALGNRNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAANGLAVGTKQEDEIKRHVHKVFSHWVNHPHAA
                     ALGYEDHNERQRSSIVSTWTDENLSDNGFLTPRPDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETVNAGALDAGTLAQGLQDKADRAHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRKSGRTSTGPRNRRRRDGLLEALQD"
     gene            complement(22363..22665)
                     /locus_tag="WGR74_000407"
     CDS             complement(22363..22665)
                     /locus_tag="WGR74_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ0904895.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
     gene            complement(22637..22924)
                     /locus_tag="WGR74_000408"
     CDS             complement(22637..22924)
                     /locus_tag="WGR74_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ0904896.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVRFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            23004..23609
                     /locus_tag="WGR74_000409"
     CDS             23004..23609
                     /locus_tag="WGR74_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904897.1"
                     /translation="MTIYFKNGFYDDTLGGIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAATRGVELDVLIEKVIEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            23588..23884
                     /locus_tag="WGR74_000410"
     CDS             23588..23884
                     /locus_tag="WGR74_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ0904898.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKILYTLINGVFFDRQHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            23881..24090
                     /locus_tag="WGR74_000411"
     CDS             23881..24090
                     /locus_tag="WGR74_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904899.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPAYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(24200..25441)
                     /locus_tag="WGR74_000412"
     CDS             complement(24200..25441)
                     /locus_tag="WGR74_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEZ0904900.1"
                     /translation="MQKWQTELYSTPLWLLQTLLIVSAASAVILFLTRKTRFGREFAY
                     ILRLCLTPKNAVKVLLLITAMVTLLLTEVRLNVLSTFMSKGLYDSMQDLNASAFWMFA
                     SMNAGVVLIRAFNNVVNDFLDQGLAIKWSERLNEVLTARWLADKNYYRLQMRRHAPDN
                     IDQRIQQDAQEFIASTIEFVRGMVNSVVTSLEFAVVLRGLAGILTVFGFDIPHGIVWF
                     VYIFVILATFIAMWIGNPLIRYNYENEKLNGDYRYSLIRVRDHAESVAFYNGEKHEYD
                     QLSDRFKAIIRNRWRIARQSVCLSGFNDMFTNGIKLFPIILQAPRLFAGQIKIGDIQQ
                     TVQAFARLQNALSFFRMFYNKFTAYRARLERLYGFLLSTEEQHSARQPEISEVSDGIA
                     LECKAAAAFARIGGSFCTAEA"
     gene            complement(25729..26376)
                     /locus_tag="WGR74_000413"
     CDS             complement(25729..26376)
                     /locus_tag="WGR74_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ0904901.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(26373..26741)
                     /locus_tag="WGR74_000414"
     CDS             complement(26373..26741)
                     /locus_tag="WGR74_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ0904902.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDNTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(26751..27422)
                     /locus_tag="WGR74_000415"
     CDS             complement(26751..27422)
                     /locus_tag="WGR74_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ0904903.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVAKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLIALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(27587..28306)
                     /locus_tag="WGR74_000416"
     CDS             complement(27587..28306)
                     /locus_tag="WGR74_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0904904.1"
                     /translation="MATLSNKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNQFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALLPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            complement(28390..29703)
                     /locus_tag="WGR74_000417"
     CDS             complement(28390..29703)
                     /locus_tag="WGR74_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ0904905.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARALAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALADLQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFTNRAWADKVHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFAAAETCLQSLSDAV"
     gene            complement(29782..30654)
                     /gene="hpnD"
                     /locus_tag="WGR74_000418"
     CDS             complement(29782..30654)
                     /gene="hpnD"
                     /locus_tag="WGR74_000418"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ0904906.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVRARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            30825..32687
                     /gene="hscA"
                     /locus_tag="WGR74_000419"
     CDS             30825..32687
                     /gene="hscA"
                     /locus_tag="WGR74_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ0904907.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTLQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDGAITRMLKDSMDNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAKELQQI
                     RQGIADLQGRLKDGKAEDIRSAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            32769..33425
                     /locus_tag="WGR74_000420"
     CDS             32769..33425
                     /locus_tag="WGR74_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0904908.1"
                     /translation="MEKSGIVYLMKTVIKGVYKIGISDVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKN
                     ETAESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSK
                     IRLFKEAYLRKSGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPR
                     "
     gene            33461..34222
                     /locus_tag="WGR74_000421"
     CDS             33461..34222
                     /locus_tag="WGR74_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ0904909.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNKPGELTNVEQGESICV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            34277..34618
                     /gene="fdx"
                     /locus_tag="WGR74_000422"
     CDS             34277..34618
                     /gene="fdx"
                     /locus_tag="WGR74_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ0904910.1"
                     /translation="MPKITVLPHTTLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(34905..35492)
                     /locus_tag="WGR74_000423"
     CDS             complement(34905..35492)
                     /locus_tag="WGR74_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ0904911.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHHQNRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLSGSGGGFT
                     VTAESVSAACPELEGRYLELVRRAAVSFGFTQGWENPAGGQEKTQAVPDAASGHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            35705..35902
                     /gene="iscX"
                     /locus_tag="WGR74_000424"
     CDS             35705..35902
                     /gene="iscX"
                     /locus_tag="WGR74_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ0904912.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(35970..36371)
                     /locus_tag="WGR74_000425"
     CDS             complement(35970..36371)
                     /locus_tag="WGR74_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0904913.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTATLREERKQL
                     DQFSRITSAYPDGRPTKRDRRQLDRLKKGDW"
     gene            36582..37541
                     /locus_tag="WGR74_000426"
     CDS             36582..37541
                     /locus_tag="WGR74_000426"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ0904914.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQVSQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLHVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            37609..38919
                     /gene="tilS"
                     /locus_tag="WGR74_000427"
     CDS             37609..38919
                     /gene="tilS"
                     /locus_tag="WGR74_000427"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ0904915.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKICVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHLLN
                     NVRALQEDLALLDEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNVQGFELHHYAGRLFVFRPEKTDKLRFLKDRQISGNLR
                     EILAGQGFVLKRHPFGLPGHLLEQDGILRTVAASDRLAVGGIHKDVKKILQEKRVLPA
                     LRPFWPVVADKDNRPLALANYGTDFQISVSDGILPVHPDFPVLF"
     gene            38970..39755
                     /locus_tag="WGR74_000428"
     CDS             38970..39755
                     /locus_tag="WGR74_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0904916.1"
                     /translation="MKHISSANNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGRPVGVYIPEAKMPSEEVRKLTAVLPEDGVFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGIGAVVLGKGCADAWSPKVLRAGM
                     GAHFLSEIYPQADLEIWLARYEDRVFATALREEKQAVLYNEDLREPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            39765..39962
                     /locus_tag="WGR74_000429"
     CDS             39765..39962
                     /locus_tag="WGR74_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904917.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLAWVWWKWF"
     gene            40224..40577
                     /locus_tag="WGR74_000430"
     CDS             40224..40577
                     /locus_tag="WGR74_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904918.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKHRFVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSAHKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            40589..40896
                     /locus_tag="WGR74_000431"
                     /pseudo
     CDS             40589..40896
                     /locus_tag="WGR74_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            40958..42334
                     /gene="mpl"
                     /locus_tag="WGR74_000432"
     CDS             40958..42334
                     /gene="mpl"
                     /locus_tag="WGR74_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ0904919.1"
                     /translation="MKHIHIIGIGGTFMGGVAAIAKEAGFKVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLSENVL
                     RHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SKSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIICNGQQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHVGVDIQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRIGGARILAVLEPRSNTMKLGTMKSALPESLKEADQ
                     VFCYAGGVDWNVAEALAPLGGRLHVGKDFDAFVAEIVKNAEAGDHILVMSNGGFGGIH
                     TKLLDALR"
BASE COUNT        10374 a        11013 c        11986 g         8999 t
ORIGIN      
        1 caacagtcaa gcggcagttg acggttcgga gggtgcgcga tgacgactta tcgtgagttg
       61 gtgcaacgca cggtcgcctg ccgccatgcg gatttagagc tgggattgag ccgcgcacgc
      121 gagcaagagc cgtttgtcat tcatgtttcc gatttgttgg ataaggccgg tatcgattac
      181 gcggtacgca tggataagga ttttcagacg accttttgtg tggagttttc cgcgacgccc
      241 ctgctgatgt gattggtatt ttgcggaaat attactcagt cttttcagac ggccaaaagg
      301 tcgaggcggc gagtcgtcat cccgaaggct acgcggtccg tatcgtattt ggcgatgtgc
      361 cggtttaaag gggttttaaa tggactttga atttggtttc agaaccctgt ggccgattgc
      421 aacggcggcg ttttggtttt gggtcaacgg catttcaggc cgtctgaaag aggcggacaa
      481 gcgcattgac gaccttaaag aggagctgca cgcggtcaag ctctcttatc acaccaagca
      541 agatgccaag gcagacagca ctaatattgc ggcagcctta gaacgaattg aaaacaagtt
      601 ggaaaaagta aacgaaaaat tggacaggaa agcggacaaa tcatgagcga cccgattttg
      661 gaagtcttgg cgcgtatcga agccaagcag gatgacatgc tcgccaatca ggctcgaatg
      721 gacgaggaat tgcagcaaat taagaaagac tgcaagaaat ctgcggcggt ttacggcggt
      781 ctcggcggtg tgattgtaac gaccggctgg gagctgctga gagccaagtt cgggggctga
      841 tatggcgcat ccgaaggaaa tccgcgaaaa gctgcgccga ttgtacgtca gcgacgggca
      901 gacgcttgaa atcgcggcga tgatgtgcga aatcccgatt gcgaccgccc gcagctggaa
      961 acgcgccgcc aaggaaagca gcgacgattg ggacaaagtg cgcgccgcct ataccttggc
     1021 aggcggcggc atcgaagact tgagccgttc gctgctggcg ggttttttgg tgcagtacca
     1081 gtcaacgatg acaatgttgc aagacacgtc gattgaaggg ctgatgccgt ccgagcgcgc
     1141 caaactgttg acaagcctgt ccgacgcgtt taccaagacc gtggcggcaa acgccaaagt
     1201 gatgccggaa acgtcaaaac tggcgacggc aatcgaggtg ttggaattgt tcggcgaagt
     1261 ggtcagggag cgatacccgc aacacttgca ggcttatgtc gagctggtcg agccgttggg
     1321 tgtggaaatt gaaaagaaat acaggtagca actgcataag gtaattgaat gaagtccaaa
     1381 gagtttttaa agtcacttgc cgaatatgcc gcccaactcc gccaaattat tgaggcagag
     1441 gtggacggct tcgatgcgtc gcccaaggct attgctgcac gccgtgccaa ggtgtttgac
     1501 ccggtaggcg gttacgagta tttcgtcaac acctactttc cgcattatat ccgctcccct
     1561 gaaaaatccg aactgcatgc gtttttattc agccgtctgc cggagattat ccgctccccc
     1621 aaaggggaaa atgaggcggt gggtgcgccg cgtggcgagg gtaagtcgac gcaggttact
     1681 cagttgttta cgctgtggtg tattgtgaca ggccaaaaac attatgccgt tattgtgatg
     1741 gacagcatag atcaggcata cccgatgctc gaagccatca aggcggaatt ggaatttaat
     1801 ccgcgcttga aaaccgactt tccggaagta tgcgggcagg gccgcgtatg gcaggccggt
     1861 acgattgtga cggccaatga cgttaaagtc caagtggccg gtagcggtaa aaagctgcgc
     1921 ggtttgcgtc acggccctta ccgtcctgac ttaactgttt tggacgatat tgagaatgac
     1981 gagcaagtcc gcaaccccga acagcgcgac aagctcaatg cgtggctgac taagactgta
     2041 ttgcctttgg gcggtgtcgg ccagaaatac gatgtgatat atatcggcac gattttgcat
     2101 tacgacagcg tacttaaccg cactttgaat aacccgtttt ggcacggtat taagtttaag
     2161 gcgatgaaac gctggcccga ccgcatggat ttgtgggaca gatgggagga acttttccga
     2221 aacgacggcg agactgtggc cgaggcgttt tatctcgcca ataaagacga gatggagcgc
     2281 ggtgcgcaaa caagctgggc ggctcgcggc gtactcgcgc tgatgaaaat ccgtgcgcgt
     2341 gacggtcatg cgacatttga cagcgaatat caaaacgacc cggtcagcgg cgaagatgcg
     2401 ccgtttgcca agtcgatgaa gttttggaac gacctgccgt ccgatttggt gtatttcggt
     2461 gcgctcgacc cgtcgctggg taaagcgggg gcgagccgtg acccgtcggc gattatcatt
     2521 ggcggttatc aacgtgaaac cggcaaactg tatgtcgtgg aaactcagat taaaaaacgt
     2581 ctccctgatt tgattattga ggacgttatc agattgcacc gtcaatatcg ttgcaaactg
     2641 tggtttgttg agacggttca gtttcaggaa tttttgaaag atgagctggt caagcgcagc
     2701 gcggcgcgtg gaatacctgt cccggcgcgg gcggtcaagc cggtatcgga caagctcttg
     2761 cggattgaga ctttacagcc tcacatggcg aacggtttga ttctgttgaa tgagagccaa
     2821 cagacgctga tacagcagtt ccgccatttt ccaaaggctg atcatgatga tggtcctgat
     2881 gcagtgcata tgctctggtc gggggcggtg gccaattgtg tgccgataga atggcaaagc
     2941 cctaccgata acgattttga tgacgagata aaaagtaaat ggagccgata atggcaaaaa
     3001 agaacaataa aactaaaatc caaaagcccg aagctgcatt gcagacggac gtggctcaaa
     3061 ttacggcgac cggtcgggtt atcgccgagc atccgtccaa ttttattaca ccgcaaaaga
     3121 tgcgggctct cttcgaggat gcggaaagcg gcgacatccg cgcccaacac gagcttttcg
     3181 cggacattga ggagcgcgac agcgacatcg cggcaaatat ggggacgcgc aaacgcgcgc
     3241 tgctgacgct caactggcgc gtcgccccgc cgcgaaatgc gacgcccgaa gaagaaaagc
     3301 tgtccgacca agcctacgaa atgatggaca gcctgcctac cctcgaagac ctgattatgg
     3361 atttgatgga cgcggtaggg cacggatttt ctgcgttgga ggtcgagtgg gtattttcag
     3421 acggccttta cctaccccga aactttatcc accgcccgca aagctggttc aaatgggaca
     3481 aagacaacgg gctgctgctg cgtacccgcg aaaatccgga aggcgaagcg ttgtggccgc
     3541 tgggctgggt cgttcatacc caaaaatcgc gcagcctcca gcaggcgcgc aacgggcttt
     3601 tccgcacgct ttcctggctg tatatgttca aacactacgc cgtccacgat tttgccgagt
     3661 ttttggagct gtacggcatg cccatccgta tcggcaaata cggcgcgggc gcaaccaaag
     3721 aggaaaaaaa caccctgctt cgagcggtgg cggaaatcgg tcacaacgcg gcaggcatca
     3781 tgccagaagg tatggaaatc gagctgcaca acgctgcaag cggtacgatg gcaaccagca
     3841 atccgttttt gcagatggcc gactggtgcg aaaaatcggc ggcgcggctg attttggggc
     3901 aaacgctaac cagcggtgcg gacggaaaat ccagcaccaa cgcgctgggc aatatccaca
     3961 acgaggtacg ccgcgatttg ctggtgtcgg acgcaaaaca ggtggcgcaa accatcgcaa
     4021 gccaaatcat cggaccgttc ctgcaaatca actatcccaa tgccgaccca aaccgcgtgc
     4081 cgaaatttga atttgacacg cgcgagccga aagacatcgc ggtctttgcc gacgctatcc
     4141 cgaaactggt ggatgtcggc gtacaaatcc ccgaaagctg ggtgcgcgac aaactggtca
     4201 ttccagatgt gcaggagggt gaggctgtgt tggtgcggca ggtaccggac aatccgataa
     4261 acagaactgc attggcggct ttatccgccc acaccgtacc atctaaggct acgggcaggc
     4321 atcaggaaat attggacggc gcgttggatg acgcgctggt tgagcccgat ttcaattctc
     4381 agctcaaccc gatggtgcgt caggcggttg ccgcacttaa tgcttgcaac agctacgagg
     4441 aggcagatgc cgcactgaat gcgctttatc cgaatttgga caacgcgaaa ctgcgtacct
     4501 atatgcagca ggccttgttt atcagcgata ttttgggaca agaccatgcc cgcgcctgat
     4561 ttgggatttg ccttaagtct gccgccaaaa aaggcaatcg agtggctgga aagtaaaaag
     4621 gttacggcgg agagctaccg caatctgaca gcctccgaaa ttgccaaagt ctatacgatt
     4681 gcccgcatga ccgacttgga tatgctcaac gacatcaaaa cttcgatggt tgaatcggca
     4741 aaaagtggac agtcgtttga cgattggcga aaaggtatct tgaatctgct cagcaacaag
     4801 ggctggctgc atccgaacgg gcataacggt aaggatatca tcgacccagc caccggcgag
     4861 gtattcggtt cgccgcggag gttggagacg atttaccgta ccaacatgca aactgcctac
     4921 aacgccggtc aatatcaagg atatatggca aatattgatg cacgacctta ttggatgtat
     4981 gacgcggtag gcgacagccg cacccgtccg gcgcattcgg caatagacgg gctggtgtac
     5041 cgctacgacg acccgttttg ggcaacgttt tacccgccca acggctacaa ctgccgttgc
     5101 tcggtcatcg cgctgtcgga gcgggatgtg gaacgccagg ggcggattgt cgggcaaagc
     5161 acgtcggaca atcttgttga gacccataaa atctacaata agaaaggcga tacttatctg
     5221 acccttgcct ataaagcacc ggatggcagt ctgtacacga ccgatcgagg atttgattac
     5281 aacgccggac gaatgaacta ccgccccgat ttagacaagt acgaccgtgc gttggcgcat
     5341 caatttgcca aagcggaaat gggtggtgcg gattttaaaa ccagctttaa acagcttgaa
     5401 aaagagtttt atgaagtcaa gcaacgtttg aatattgatg gcaagcccga taaagagcag
     5461 aaaatcaaaa tccgaaacgc gctatcaaga cagcttaaat ttgctgcggg tgtattgagc
     5521 aaggaaatgc aagaactggc aggtatgact cgagcgacgg tgtggctgtc tgatgataca
     5581 ttggtcaaac aggtggacag tcgggagggg caatcgtttg gaacagattt atatgccatg
     5641 ctccccgatc tgattcatga gcccgaatat attttttatg acaaaagcag aggaaaagaa
     5701 agttttgttt tgctcggaaa accgttggag gggaacgaaa aatacagatt gatggcagtt
     5761 ttgaaatatc tgccacagta cgattcgatt tttttggatt cgtttcgcag ggcggatgaa
     5821 aaagaaattg tgaaaagtaa gaaacgctat gaaatgaaaa aaggctacta agtcggactg
     5881 caatcccgac acactctcgc ttatgtaacg gataccaatc ccagtccgag gactgcataa
     5941 gggctgcgga gggcagacaa attcaccgct tttttagtag cctaggcagg ataataccat
     6001 gattgatgtc aaaatagaca atatctttgt cgtcctaaac caaatcgagc ggcttggcaa
     6061 cgggatcgaa aaccgctacc tgctgatgcg ccgactgtcc gaaaccatgc acacggcggt
     6121 caagctcaat ttccgctacg caggccgtcc gaaatggttg gggctaaaat accgcgacgg
     6181 caagccgctt tcggattcgg gtcgtctgaa agacagtttt tccacactgt cagacaacga
     6241 tacagccctt gtcggtacga atatcgtcta tgccgccatc cacaacttcg gcggtatggc
     6301 ggggcgcaac cgcaaagttc ggattccgca acgggaattt ttgacgctga cggacgacga
     6361 caaacaggct ttgatggacg atgtgcagga ttatttttcg ggtctgatac cgtgaattta
     6421 taaaaccctc aaaaacgcgc tttttagcgc gtttttttat gcgggtaata caaacccctg
     6481 cccaagatat aaaaatcaat cctagacgct tctaaaaagc ccctgaaaac gattaattgt
     6541 gtatcgcgcg gacaggtttt aaaaaaatgg cgggagggtt tgaagcacgc ctactctttg
     6601 ttgttttttc aaataggcaa aatgaccgta ttgagagagg tacacatgtc caaaaatgca
     6661 caaaaaaccc tacttgccgt gtgcagtttc gaggtgcagc caaaagacgg gcgaatccaa
     6721 ctgctgccat atggcgaatt tcgcgcagta gacggtcgtc cgactgatgt ccctgcgtgg
     6781 tatctgaccg aagaaaacgg tcatgatgtc gcgttgttgg ccaacagctc gcgcaatcag
     6841 ttggttgtcg attatgaaca ccagacgctc tacaaagaga aaaacggaca acctgcacct
     6901 gccgccggtt ggatgcgttg gctggagttc acgcctaaag gcatgtttgc cgaagtggag
     6961 tggacggaca aggcggctgc ggcaattgcc gcaaaagagt atcgctacat ctctgctgtg
     7021 ttttcctatg acacaaaggg atatgtaagc aaaatttttc acgccgcgct gacaaatttc
     7081 cccgcgttgg acggtatgga cgaggtgctg gcggcagcgt cggcgcaaat tttaaaaccg
     7141 gaaacggagc aaaaccctat gaaagagttg ttacagcaac tgttcggtct gcctgatgcg
     7201 ggcgaagaag aactgaaggc ggcattgtcc gcgctcgtgg aagccaagcc gaaagacgtg
     7261 gcattgtctg ccgacgtgtt cgcgcagctg gcggaaaaag acagccgcat cgcggcattg
     7321 acggcgcaaa ccgccaagcc tgatttgact aaatacgcgc ctatctcagt ggttcaagag
     7381 ctgcaaagca aagtcgccgc gctgacttca ggcaaattgc tgcctgctca gaaggagtgg
     7441 gcagaaggcg tattgaaaca gccgggcggc ttggcatttt tgaccggctt tattgaaaac
     7501 gcccagccgg tcgctgcact ggcaggctcg caaacgggcg gtaaagcacc cgacgaacgc
     7561 gtcgccgcac tgactgcgga agaggcagcc gcagcaaaaa tgctgggcat gtccggcgaa
     7621 gaatttgtaa aaatcaaaga aagcgaaggt aagtaatgga caaatcagcg attttgaccg
     7681 caatcacggc agcattccgc aaagaatttc aaacgggttt ggaatccgtc aagcccgact
     7741 ataccgccgt tgcgatgacc attccgtcca atacggcaac caatacttat gcgtggctcg
     7801 gcaaattccc ccaaatgcgc gaatgggtcg gctcgcgcca aatcgaaaaa atgaacaagc
     7861 aggcgatgag cctcgacaac aaaaagtttg aagcaaccgt cggcgtagcg cgtacggaca
     7921 tcgaagacga ccaagtcggc atgtaccgcc cgatgatggc ggcaatgggc gagtccgccg
     7981 ccgccctgcc cgatacgctg gtgtggggtc tgctcaaaaa aggtaaaacc actgtcggtt
     8041 atgacggcca atatttcttc gataccgacc accccgttta cgagaaatcg gacggcacgg
     8101 gtcagaatac gccgcactcc aatctgacga ccggtacgga caacgacgcg ccgaccttct
     8161 acgtcgtcga tgacaccaag accctgaagc cgctgatttt ccaaaaccgc accgactccg
     8221 agtttgaaac caaattcgac ccgtccaaat ccgaccgcgt gtttatggaa gacgaatacc
     8281 tgtacggatc gcgccgccgc tgcaatgccg gtttcggttt gtggcaactg atgcacatgg
     8341 cggaaaaaac cgcgctgacc cgcgaaaacc tcgccgccat tatcgtcaaa atgcagaaaa
     8401 tcaaagccga cggtggctat gtgttgaatg tgaagcccag cctcttggtc gttccgcccg
     8461 agctggaaga caaagcccgt gaattattgg aagccgacaa aatcaacggc acgaccaata
     8521 ccttcaaagg ccgtctgaaa ctgcacgttt gcgtccacct gtaaccccga accgttttca
     8581 gacgaccttt aacacctatt taaaggccgt ctgaaacaag gagtcatcat ggcaaaaacc
     8641 aacaacaaac cagaaaccgc cgaaaccgcc gccccgtcgt ttgaagacat caaagccgaa
     8701 ttggacgccg tgcaggcaga gcttgccgcc gcccgaaacg atgtcgaaat gctgaccaca
     8761 gccttggaaa aagccgaaga cgacaaaaag gcactgtccg ccgaacttgc cgaactcaaa
     8821 gtgcagcata cgcaacgcgc cgccgacgct ttggcggaca gccgcgatgt gatgctcgtc
     8881 agtaccggcg cagacggcaa agaattttgg cgcggcggcc tgctgtttga cggcggctgg
     8941 cgtgaagtga agcgcgccga agtcggcgaa gcggtgtgga aggcaatctg cgccgagcct
     9001 atgctgcaac gcaaggcggt cgagtaatgg catacgcgac ggttgaggat atggttgcgc
     9061 gtttcggtga gctggaagtc ttgcagctca ccgaccgcaa caacgagggg ctgattgacc
     9121 gcgaggtcgc acaaaccgcg ctggtggacg ccactgccga aatcgacgcg tatctggggc
     9181 ggttcagacg accttttgag gatctgccgc ccatcttggt gcgcctttgc tgcgacattg
     9241 cccgctaccg tctgacggcg gctcagggcg tgttgattac cgacgaaatc cgcaaccgct
     9301 acaaaatcga cgtgctcgac ctgctgcgtg ctatggccaa aggcgaagtg cagctgggcg
     9361 tggatgatag cggcgaagaa gtggccgcgg gcgaagacgg tattgtgttt gtaaacggta
     9421 aaaataaggt gttcgggcgt gatcactgat attgagcaag cgataacaga ccgtctgaaa
     9481 cggggcttgg gtcgcatggt gcgcacggtt aaaagctaca acggcgaggc cgacgatttg
     9541 gcggggcaaa tccatacgct gcctgcggtt tgggtaacgt atggcggcag caaagttgag
     9601 cctgccagca ccggcggcgt atgcggacgt tatcaggata ccgccgaatt tgtggtgatg
     9661 gtggcggccc gcaatctgcg caacgagcag gcgcagcggc aaggcggcat cgacagccgc
     9721 gaaatcggca gcaacgattt aatccgcgct gttcgccgcc tacttgacgg ccagcggctc
     9781 ggttttgccg atagccgcgg cttggtgccc aaagcggtgc gcgcgattgc caatcatgtg
     9841 ctggtgcaaa acgccgcagt aagcatatat gcggttgagt atgccatccg ctttaacacc
     9901 tgcgggttgg aaaatgaccg ctaccccgaa cgcaccgaca atcccgacga ccccaaccat
     9961 atctttacca agtatcaggg tacattgagc gagccgtggc ctgatttcga ggggttggac
    10021 ggcaaaattt acgacccgca atccgccgat gaaatacctg taaacctaac ccttaaggat
    10081 aagcaatgag caaaatcaaa gtaacggcgg cagatggcct gcgtgtgccg accgaacaca
    10141 acccgcacga atatatcggc caagagccgg tggaggtgga cggcaacagc ctgtattacc
    10201 gccgcatgat tgatgacggc gatttggtgg tggttgagga tgccgcccca aataccaaaa
    10261 cccgcaatac taagggagag taatgatgcc ccatattgat tttgacacga ttccgggcag
    10321 catccgcgtg cccgggcagt atattgaatt taacacccgc aatgccgtgc aaggtttgcc
    10381 gcaaaatccg caaaaggtat tgatggttgc acccatgctg accgcgggca tacagcccgc
    10441 cttagagccg gtgcaactat ttagcgatgc cgaggcggcc gatttgttcg gacaaggctc
    10501 gctggcgcat ttgatggtgc gccaagcatt tgccaacaac ccttatttgg atttgaccgt
    10561 tatcggtatt gccgaccaca gcgcaggcgt gcaggcaacc gcaaccgtta ccctttccgg
    10621 cacggccacc gcgccgggcg tggtggaaat cacgattggc ggcaagcagg taagcacggc
    10681 cgttaacacc ggcgagaccg ccgccacagt ggcaggccgt ctgaaaaccg ccatcactgc
    10741 cgccgatgta accgttaccg catccggcag cggcgcagcc gttacgctga cggccaaaca
    10801 caaaggcgag atcggcaacg agagcggctt aaccgtgagc accggcaata ccggcctgac
    10861 ttatcaagcc aatgtcttta ccggcggtgc caaaaatgcg gacattgcca cggccttgtc
    10921 caaagtggcg ggcaagcatt atcacattat ttgcagcccg tttagcgatg acgccaacgc
    10981 caaagccttg agcaaccata ttaccaacgt attcaacgcc atcgagcagc gcggctgtat
    11041 cggcgtattg ggtatgagtg cggccttgag cacggccacc accgctaccg gcaaaatcaa
    11101 cgacggccgc attacctgtg cttggtacaa aggtgcggta gagccaaacg gcatcatcgc
    11161 cgcaggttat gcggcggtgt tggcctttga agaagaccct gccaagccgc tgaacacgct
    11221 ggaaatcaaa gggctggccg ttacacctga tgcgcaatgg ccgtggtttg cagaatgcaa
    11281 caatgcgctg tacaacggct tgaccccgct cacagcggtc aacaaccgcg tgcagattat
    11341 gcgtgccgta tcaacctata ccaagtcggc caacaacacc gacgacccgg cactactcga
    11401 cattaccacc attcgcacgc tggattatgt gcgccgcagc gttaaagagc gcattgccct
    11461 gcgttttccg cgcgacaaat tgagcgaccg cctgctgccc aaggttaaga gcgagatttt
    11521 ggacgtgctg attaagctcg accaagccga aatcatcgaa aacgccgagg ccaacaaagg
    11581 caagctggtg gtggcgcgtg cgcaaaacga ccccaaccgt gttaatgcca ttatccccgc
    11641 cgatgtggtc aacggcctgc acgtctttgc cgggcgcatt gatttgattt tgtaaccctt
    11701 ttcagacggc gtttaaaaca ggtttaaagg ccgtctgaaa ccttaaaaaa ggataaagca
    11761 tgagcgacgc tacctatacc ggcgcggtga ttatggaggt gaacggccgc gatatcgaga
    11821 ttgtgagcat caaaccgcaa accactacag gccgcaagcc ggtcaaaacg atgaaccgca
    11881 acggccgagt caacggttat tgtgacggcg taaccgaaca caaattaagc gttaccgccg
    11941 ccattccgat cgacggtacg gaaatcgact gggacaacat caccaaggcg aaaatcacga
    12001 tttaccccat caacgacgaa ggtcgccgca cttcctacct cgactgcttt accgtcgata
    12061 ccagcgagca atatgaagtc gataacgagg cacgcatcga cattgagatg attgctttgc
    12121 acaaaatcaa ggagtaatgc gtgattaccg tcaaactgac ccacgggctg acctacaatg
    12181 gcaaagtcgt atctgaatta cgcctcaagc cactgaccgt cggcggcgaa ctggcggcgt
    12241 tcgccctgat tgatgacttg cccgagctgc ccgaaaacgc cacaaaagcc gaactgctgc
    12301 aacgcgacgt cctagagacg ctgacctact ggtcgcagca gattgaagcc caaggtatcc
    12361 catccgacat cctgacggcg cagtggctga tggaaaacct ctctaccgaa gactaccata
    12421 ccgtgatggc ggctcaggag gatttgtgcc taaaaccgtc cgccgctacg gcgagccccg
    12481 acgcgccgtc ggcggcggag cagtaaaacg cagctacctg acggcacaca aaagctaccg
    12541 tcaggcggtc atcctgatgg caaaggcggg gataggggcg gacgcagtgc gcgatatgtg
    12601 ccacgccgag ctgtcggcat ggtttgaaga catcctgtcg agcctgggca taaaaacgcc
    12661 gaaggaagag ggagtgattg tgtcgaaacg gctgagaaag ccggagggaa aataaaaagg
    12721 tcgtctgagt ttcagacgac ctttttttta tttgacggtt agggttgttt tctgccgatt
    12781 attgcaatgg tgtttgtttc tttttcaaaa acaacgctat ataaaatacc atctgccgga
    12841 acgtcttttt gcgctgttgc tcctgtctga ttggattctt tgacgaagtt gttttcctgc
    12901 ttccgattcg cccaatttgt ctgtggtagt taaagacaga actgcctgaa tactgtgtaa
    12961 tccatttatg gcattattga ctagattttg tcctttctct ccgtcggttt ttaaaatgta
    13021 gcctactgag atagctctaa ttttttgctg ctcgtccagt ttgagcgcaa tcgccccaaa
    13081 atccattacc aaagtaaggt cataaccaca atcggtcttg acaatatttt tattatggat
    13141 ttttgttgag acgttctgag ttttaaatgc ttgctccaaa ttgatgagat attgttcaac
    13201 tgtgatgtcc attggggcgg tacaatcagc agcctggata ctttggacag gttcttcggc
    13261 ttgtgccgtt tttgattgtt ggttgcaggc tgataaaaca attgaaacac aaattgcgga
    13321 aatcaatttt ttcatattca taaaatatcc ctttgaataa aatggttatc attctggtat
    13381 tataacgcaa caaacaaata aagcacgaaa acggggttga agcccatacc gcctccctta
    13441 aacagccttt aaacgataat tgaccttgag ttaatacgtt taaaggctgc tttttatggc
    13501 aaacgggaac atgaaactgt cgttggtgtt aaccgcccga gatgacggag cgagacggct
    13561 actggctgat actcaacgac aattagatcg taccgcgaaa tcgcgggcgc aacttgaacg
    13621 gcaaagccat acttatgcgt tgaccggcat ccgctcagaa aaacagattc aacgcgaaat
    13681 catgctgaca caggctgcgt ttaaccgttt ggcgcgcagc ggcaaggcat cacaaaatga
    13741 tttggcacgg gcggcggtcg ctacgcgtaa ccgaattcgc gagctgaacg cggaactgaa
    13801 acagggcacg ggatttgcgg acaagatggg aaaaatcgga agattcggtg cagctgcggt
    13861 ggctggtggc gcggcagcgt atacggtgct taagcctgct atggacaaca gaaagcagct
    13921 tgatgagaac atcaaccgcg tgtccagaca ggcatttatt gaggataaca gtaaatcggc
    13981 agcgtggatt gcaactgaag gtgcgcaaca gatcaaggat ttggcacttg aacttgtcga
    14041 gaaaaatggc gggacccacg ataaggcttt ggatttaatc agcggcatga tgaccaccag
    14101 tctgaatttt gcccaaacca agaatgaagc gcaggcggca tatgcttttg cacttgcctc
    14161 agaaggcagt ggcgaggata cggcaaaact gattaaaacc ctgaaagatg gcggcatgag
    14221 cggtaaagac ctgcaactcg ggcttgagca cgtcttgcaa tcgggtttag acggcacttt
    14281 cgaggtgcgg gatatggttc gggagctgcc gagtctgctc cctgccgcgc aacaggcagg
    14341 gatgaatggt gtcggcggtt tggactacct gctctcactc ttacaatctg cggcgaataa
    14401 atcgggcagt cctgccgaag cggcgactaa tgtgcaaaat cttttgagta aaactctgtc
    14461 gccttacacg ataggtcgtc tgaagaagat ggcaaatcca aatgacccga agaaaggtgt
    14521 cgattggata ggctcggttg tgcaaggcaa gcaaaacggc gaaaacgcag tgcaggtgtt
    14581 gtcccgtctt gccgatgcca tgctagtaaa ggataagcaa taccaagatt ataagaaacg
    14641 cgcggctgca ggcgataaga cggcggcgga gcaggcaaat atgcttaagg gcgcgctttt
    14701 ggcgcaactg ctgcctgatt tgcaggcaaa acaaggtttg ctggctgcaa cggatatgac
    14761 gcaaatccgt gaatatatgg cttcgttggc tggcgtaacg ttggataacg gaaaaattgc
    14821 taagaacaac gaggcgcgaa tgttgtcggc agcggcgcaa caagagcaac aggaatcgct
    14881 ggcaatgttg cgggaaagtc tgacgggaac attggtggat atggaaacct cgtttaaaaa
    14941 gctggcagcg gaatacccta atgccactct agccctgcaa gcattgacga cggcggcaac
    15001 agcggcgtct gccgcaatgt tattaaccgc cggtggcggt aaaggtgcag gctttctgaa
    15061 agatgtaggt agtaaagcgt tggtatgggg taaggcttcc gcaggcggcg tggcagcagg
    15121 tgccacagcg gcaggcggta agttgctgtc atggggaaaa tctgccggta gcgggctcat
    15181 gaataatcca gcgttagtta aacgggcggg tttgttaggt atgttgctgt attccgagtc
    15241 tttgggtgac ggcacattgc caaagggttt gcgtggtacc aagacaactc ctgaaatgat
    15301 taatcgtctg aaaaacaacg gtatccgatt tgaacctgcg ccgaagcggg aacaggcgcg
    15361 gggtggtgtc cctcagtatt tggctgctcc gtcagcgcag cctaccgata agatgttgtc
    15421 tccgttgttt tcaactcaga cggcggcgta tcaggcagcc attcagcagc agacggcggc
    15481 gtatcaggca gcattggcgc aggatacggc tgcagttaca acaggtttgg cacaagtgca
    15541 aagtgcgatg gcgtcggcaa gtcagaccat caataccaat gtgagcctga atatcgacgg
    15601 acgtgttatc gcgaatgagg tatcgcggta tcaagtggcc atgttcggcc gtggagcggg
    15661 tcaataatga gcggatggca taccttattg caggacgcat cttacaaggg cgtcggcttt
    15721 gatattgagg tggtggacga gagcaacggc aaggcattgg ccgagcatgc gcggccgttt
    15781 gtgcagggta tcgaccttga agacatgggc atgaccgggc ggcaggtgca gattaatgcg
    15841 gtgttttggg gcaagggcta tgcaggccgt ctgaaaaagc tgctggatgc gctggagcag
    15901 ccgggcggcg gcgtgctggt gcaccctgtt tgggggcgga tgcacaacat gattgcggca
    15961 tcatggagtt accgacatga ggccgattat gtggattatg cgggcatcga tattactttc
    16021 cgcgaggcgg ccgaagcgca ggaaatcttt gtttttgaaa acgccttttt ggtcgagctt
    16081 gaggcgttga ttgctgatat cgacacctac cgcgaggcgg ctatcggctt tgttgatgcg
    16141 gtgttggcgg tggatgcggg cgtatcggct ttatggggca gcgcgctggg catttggagt
    16201 gcggcatcgg gtacgtttgg tgcggtgcgc cgtttgtttg atttggacaa aattgccttt
    16261 cccgatcggg gcggatacag cgcagcggcg tttaaaaacg gctcggccaa gctgtttgcg
    16321 gatatatcgg tcatggtaga tactggcata cgccgtgagg cgggtttggc cgataatgcc
    16381 atgcaccatg ccggttggtc gccgcgacag cggtttgacg gggctgcggc tgttgccgac
    16441 cgtgccgccg ctatccctga taatttgctg accggccgct tttcagacgg cctgcaaaac
    16501 cgcctgaacc gtttaaccgc caaacaggtg cagccggtag cgcaggcggt gcgcctgtta
    16561 tccacgtcat cgctgttgtc ggtggcaacg gcattaatcg aggcgcatgg cgaagagatg
    16621 accgcgcccg atttgattga ggttaaccgc gccatgcgcc gccgtatgca ggccgagatt
    16681 gccgccttac gggcggtgca gacggctgct gccgagtctg gtgggctgac ggccaacgcc
    16741 gtgtataccg aggcttacca aacggcagaa tccctgcgcg cggcggcagg ccgtctgaat
    16801 gcgttggttg cggcggtcat caaccaaaag ccgccgctga ttgtgcgcca agccccaatc
    16861 gacggtacga tacaccaaat cgcccacgag ttttacggcg atatagcccg cgcagcagag
    16921 ctggtgcggc tcaatcccca tatccaccac cccgcgttta tcaagcgcgg cactttggtc
    16981 aacagctatg caaaataatt catacggcta tgccgtgtcg gtgcgcgtgg gcggtaaaga
    17041 gcaccgccac tgggagcgct acgacatcga cagcgacttt ttaatccctg ccgacagctt
    17101 cgattttgtc atcggcaggt tggggccgga ggcggccata cccgatttaa gcggagagag
    17161 ctgcgaggta gtgatagacg ggcaaatcgt gatgacgggc atcatcggca gccagcgcca
    17221 cggcaaaagc aagggcggcc gcgagttgag cttgagcggg cgtgatttgg ccggtttttt
    17281 ggtggattgc tccgcgccgc agctcaatgt aaagggcatg acggtattgg atgcagccaa
    17341 aaagctggcc gcgccgtggc cgcagattaa agcggtggtg cttaaggccg aaaacaaccc
    17401 cgctttggac aaaatcgaca tcgagccggg cgaaaccgta tggcaggcat taacccatat
    17461 tgccaactcg gtcgggctgc atccgtggct ggagccggac ggcacgttgg tggtgggcgg
    17521 tgcggattac agcagcccgc cggtggcgac attgtgttgg agccgcaccg acagccgccg
    17581 caatatcgag cgcatggaca ttgagtggga taccgacaac cgcttttccg aggttacttt
    17641 tttggcgcaa tcgcacggcc gcagcggcga cagcgccaaa cacgatttaa agtgggtgta
    17701 caaagacccg acgatgacgc tgcaccgccc taaaacggtg gtggtgtccg atgccgacaa
    17761 tttggccgca ttgcaaaagc aggctaaaaa gcagctggcc gactggcggc tggagggatt
    17821 gacactcacg ataaccgtgg gcggccataa aacccgcgac ggcgtattgt ggcaacctgg
    17881 ccagcgtgtg catgtgatcg acgacgagca cggtatcgat gcggtgtttt ttctgatggg
    17941 gcggcggttt atgctatccc gcatggatgg cacgcaaacc gagctgcggc tcaaagagga
    18001 cggtatttgg acacccgacg cttaccccaa aaaggccgag gcggcgcgca agcgcaaagg
    18061 caaacgcaaa ggcgtgagcc ataagggcaa aaaaggcggc aaaaaacaag cagaaacggc
    18121 ggtgtttgaa tgagtttgag taaattggcg aaaaaaacgg cacaaactgc taaaaatatc
    18181 ggcgaaaccc tgcgcgcggc ctttcgggga aaaatcacgc tggtggtgtc gtccgagccg
    18241 atacagcgcg tgcagttgag cggcttggcc gacgaaaccc tgcaagacct tgaacatttg
    18301 caggaatacg gctttgccag ccatccgccg accgcccgca ggcggcaacc gttacgacta
    18361 taaaaactgg gcgttgagtg ttgacggcgt aaccagcgca tatgtttatc cgctgcgccg
    18421 cggcttgggt acggtggata ttgccattac ctccgccgac ggtgtgccgt cggaagaaac
    18481 tgtgcgccgc gtacaggctt atatcgacga gatgcgcccg gtaacggcaa aaaatgcgct
    18541 ggtactcaag ccaaccgtaa cggcggtgcc tgttaccgtg caagtcaagc tcgacggcat
    18601 cgacttggac gatgccaagc gccgcatacg gacggcccta aaagaatatt tcgacaccct
    18661 gatccccggc gacggcctga ctgtgtcgca aatcgaggcg gctatcagca atgtggatgg
    18721 tgtgatcgac cgccgtctga ctgcgccgac ggccaaccgt gccgccgata cggttaaccg
    18781 catcgagtgg tttaaagcgg gcgcgattaa tgtaacggag atgccgtcat gagctatcaa
    18841 gacatcttgc ggggtctgtt gcccccccgt gtcgtatgcc cgcaatgccc cgcgtgtgcg
    18901 ggcgcaggca gaaatagacg gcgcagcgct ggatgcggta gcggaatcgg ctcaaagcgt
    18961 tgccgatgcc gtcgacccgc gcagcgccgg ccaaatgctg gccgattggg agcgcgtatt
    19021 aggtttggac ggtacgggca aaaaccgcca gcgccgtgtg ttggccgtca tggccaagct
    19081 aaacgaaaca ggcggcttga gtattcctta ttttgtgcgt ttggccgagg cggcgggcta
    19141 tcaaatccaa atcgacgaac cgcagccgtt ccgcgccggt gtcaaccgtg cgggcgaccg
    19201 tcttgcgccg caggaaatca tgtgggtgtg gcacgttaac gtgcgcggcg gcaacaaccg
    19261 cattacccga ttccgcgccg gtatctcggc ggcgggcgac aggctgaccg attacagcga
    19321 tgccgtgatc gaaagcctgt tcaaccgcct caagcccgcc cacaccgcta tccgatttac
    19381 ctaccgataa ggactaaacc atgcacccca tcgaaacccc cgataagacc ttccacgacg
    19441 gcgacggcgt gtccgaattg ggcaccatcc tgcccgcgtg gtggctcaac caagtgcaat
    19501 ccgagctgct ggccgtgctg actgcggccg gtatccagcc ggataagtcg cagcctaatc
    19561 aattactggc agcactgaat agactggctg tagttaccac cggcaaccaa gaaatcggcg
    19621 gccaaaaaac ctttaccgcc gcaacccaat tccaaagcgg catccattta tccgccaacc
    19681 agacgaactg gaacggcggc cacaaagcct acatcggcgc ggatgccgac aacgcccaca
    19741 tcgtcttcgg cgacgacacc ctccgcctgc acagcgcaaa caaccgcatt tcctacaaca
    19801 accacgacat cttccacaaa gccaacaaac cgcgttttgc cgaagacatc gaaggcaaac
    19861 cgaacacact gtccggatac ggcatcggca atttcaaagt cgaaacattc cggggcgatt
    19921 tgaacaccct caaaacagac ggcatctatt ccctgccgac agcgggcggc agctccaacc
    19981 tgcccgttga aaacactgcc tgccatatcc aagtcatcgc cggcacgaaa cacggctggt
    20041 gcaggcagtt gggttatccc gcctacacgt ccgacgtgta cgaacgccac caaacgagca
    20101 gcgcaaacga caactggtcc gcatggaaaa aactcaattc ggacggcatc cccgtcggcg
    20161 cgatcgtatc ctttcccaaa gccgtccgaa accccgcagg ctatctcaaa gccaacggca
    20221 cgacctttgc gcaaaacacc ttccccgacc tctaccgcgc cttgggcaac cgcaaccgcc
    20281 tgcccgattt gagccgtacc gacatcggca tcaccgcgtg gtttccgtcc gaccaaatcc
    20341 cgaccggctg gctggcgttc gacgacatcc gcacgcgcgt aaccgaaacc gcttatcccg
    20401 agctgtaccg cctgctgacc ggaaaatacg gcagcatcca aaacgtcccg caggcggaag
    20461 accgctttat ccgcaacgcc gccaacggct tggcagtcgg aacgaagcag gaagacgaaa
    20521 tcaaacggca cgtccacaaa gtattttcac actgggtaaa ccacccgcac gcggcagccc
    20581 tcggttacga agaccacaac gaaaggcaga gaagctcgat cgtatcgact tggacggacg
    20641 aaaatttaag cgacaacggc tttttaaccc cgcgcccgga cagcaaaatg gcaacgggcg
    20701 gcgacgaaaa ccgccccaaa gccctggttt taaaactgtg catcaaagcc gccgacacct
    20761 tgggcgaagc cgtgttttgg ataaagtccc acggcgaaac cgtcaacgcc ggcgcgctgg
    20821 acgcgggcac gctggcgcaa ggtttgcaag acaaagccga ccgcgcccac acccacaccg
    20881 ccgcccaaat ccaagggctg gacgaaaaaa tcagcaccgc cgttgccgcg caattcacac
    20941 gccaaaccat cggcggcgtg gatattgtca gattccccga cggcacaatg atacagaccg
    21001 gcagctacag gttcacacga agcggcggcc ccatcgaaaa cgaagtcgtc ttccccgtcg
    21061 cctttgccga cggcaacgtc aaatgcttcg tatccgaacg ccattcggga cgcgttaccg
    21121 gcgatcgaag gcaacacaac tggctgttta tccgcgcaaa aaaccacgcc gccgccatta
    21181 ttaccaactg gtacgaaggc agttgcgact ggatggccat cggcaaagcc gcctcgggaa
    21241 acgccgccag ctccccgata ggccccgaaa tacctgaaac caacgaagaa ccgcaaagaa
    21301 agagtggaag aacatcaacc ggaccccgaa accgccgccg ccgagacggc ttgctcgagg
    21361 cactgcaaga ctagggggct gtagagatgg ctgtagagac gggctgtaga gatggctgta
    21421 gagacgggct gtagagatgg ctgtagagac gggctgtaga gatggctgta gagacgggct
    21481 gtagagacgg gctgtagaga tggctgtaga gacgggctgt agagatgggc tgtagagatg
    21541 ggctgtagag atgggctgta gagatgggct gtagagatgg gcttcagccc gccgatccaa
    21601 gcaatccgac cgaaaccggc cgcgccgcca atcccccgaa acctatgccc cgccaatcct
    21661 gccactcttc gtcattcccg ccgctttcgt cattcccgcg aaagcgggaa tccagcccct
    21721 aaacgcggca ggaatctatc ggaaaaaaca gaaacccccg ccaccgtcat tcccgcgaaa
    21781 gcgggaatct agaccccaaa cgcggcagga atctatcgga aaaaaccgac cccccgccac
    21841 cgtcattccc gcgaaagcgg gaatccagac cccaaacgcg gcaggaatct atcggaaaaa
    21901 acagaaaccc ccgccgccgt cattcccgcg caggcgggaa tccagacccc aaactcggca
    21961 ggaatctatc ggaaaaaaca gaaacccccg ccgccgtcat tcccgcgcag gcgggaatcc
    22021 agaccccaaa ctcggcagga atttatcgga aaaaacagaa acccccgccg ccgtcattcc
    22081 cgcgaaagcg ggaatccaga ccccaaacgc ggcaggaatc tatcggaaac ggctgaaacc
    22141 gaacggactg gattcccgct ttcgcgggaa tgacggcggc aggggtttcg ggattcccgc
    22201 cttcgcggga atgacggaaa gtggcgggaa taacgaaagg cgggaatgac cgcgcaaaaa
    22261 gccgctgccc ccttcggacg gcaccggcaa caaaaaaccg cacggccgaa accgcgcggg
    22321 aaaggatagt cgggcgcgcc cgataagcag cggccgcccc tgttatttca attgggcgac
    22381 atattggcgc aaaacctcgt tgatgcgcgt ctgccagccc ttgccgccgg cgcggaattt
    22441 ttcgaccaca tcggcggaca ggcgtatggt aacgagttgt ttcggggttt tgcctgtgtt
    22501 tcgtttttgc attacgccct tttcttccaa ttgtttttga tgggaaaaaa gcacctgtgc
    22561 caagtcttcg ggcagggctt cggcaatggg gcgggcaagc gcaaaatctt cggcggcaag
    22621 ttcccgcact tcgccgtcag cgtttgttaa ggattgacgt tgcatatttt ttaacctctc
    22681 ttttattcgc tttgcgaaaa ctgatgacac ggatgccgtc ttttatcggc gtaaaacaga
    22741 caatgtgcag gcgttgcgta tcgcctagat aagcagcggc aacataacgc ggttcggggt
    22801 aatcaaagcg gacatcgggc acaataacgg ccgttgtcca gcgtatttgc ccgactgatt
    22861 caaagggcaa attccgctct tcgatattgc gttgattttt ttcggagtca aattcaatct
    22921 tcattgcagc ttgcagcgta ttttgtcgtt acattataag cggcaaaaaa ccaaaatgta
    22981 aatacaaaaa aggaaacccc gaaatgacca tctatttcaa aaacggcttt tacgacgaca
    23041 cattgggcgg catccccgaa ggcgcggttg ccgtccgcgc cgaagaatac gccgcccttt
    23101 tggcaggaca ggcgcaggga gggcagattg ccgcagattc cgacggtcgc cccgttttaa
    23161 ccccgccgcg cccgtctgaa taccacgaat gggacggcaa gaaatgggaa atcggcgaag
    23221 ccgctgccgc cgcccgtttc gccgaacaaa aaaccgccac ggcattccgc ctcgcggcaa
    23281 aggcggacga actcaaaaac agcctcttgg cgggctatcc ccaagtggaa atcgacagct
    23341 tttacaggca ggaaaaagaa gccctcgcgc ggcaggcgga caacaacgcc ccgaccccga
    23401 tgctggcgca aatcgccgcc acaaggggcg tggaattgga cgttttgatt gaaaaagtta
    23461 tcgaaaaatc cgcccgcctg gctgttgccg ccggcgcgat tatcggaaag cgtcagcagc
    23521 tcgaagacaa attgaacacc atcgaaaccg cgcccggatt ggacgcgctg gaaaaggaaa
    23581 tcgaagaatg gacgctaaac atcggctgaa aaaatacgtt taccacctgt tggtagccat
    23641 cgaccaactg ttcaacgccc taaccggcgg cgcggcggac gaaaccctct caagccgcac
    23701 ctatcgcggc gcgcggctcg cccaaaagcc caaaacccgc tggaagattt tatataccct
    23761 gattaacggc gtgtttttcg accgccaaca ctgtcggcag gcgtatatca gcgaactgaa
    23821 aggcaggcag cacgacgcgc ggttcaacca aagccgcgcc gccggggaaa aggggacgcg
    23881 atgaactact tcggcatggt agagtttctg cgcctgatgg caatggtgcg gccgccattt
    23941 gtcttcttct ccggcacccg cagcgaactg cctgcttatc ttgacctcgt ggcagaactg
    24001 cgcctgaccg gatgggagcg ttttgcaggc agccaaacct tgactgtgag cagcacatca
    24061 acagcaattc aagctacgac gaccacctga tttataagtt ctgaccgcaa gtagcgtact
    24121 acttttaaag gcataagata atccccgttt aacagaccat taaacgggga taaatttgtg
    24181 caaaagctaa tacaatttcc tacgcttcgg cggtgcaaaa gctgccgcca attcgtgcaa
    24241 aagctgccgc cgccttacat tccagtgcaa tgccgtctga aacttcgcta atctcgggtt
    24301 gtcgcgcgct gtgttgttct tcggtactca gcaaaaagcc gtacagacgc tccaatcggg
    24361 cgcggtaggc ggtgaatttg ttgtagaaca ttcgaaagaa agaaagcgcg ttttgcagtc
    24421 gcgcgaaggc ttggacggtt tgctggatgt cgccgatttt gatttgtccg gcaaacaggc
    24481 gcggagcttg caaaataatc gggaagagtt tgatgccgtt ggtgaacata tcgttaaagc
    24541 cgcttaagca gacgctttgc cgcgcaatgc gccaacggtt gcggataatg gctttaaagc
    24601 ggtcggaaag ctggtcgtat tcatgttttt cgccgttgta aaacgccacg ctttcggcgt
    24661 ggtcgcgcac gcggatgagg gaataacggt agtcgccgtt gagtttttcg ttttcataat
    24721 tgtaacgaat caaagggttg cctatccaca tggcgataaa ggtcgccaaa atcacaaata
    24781 tatagacaaa ccaaacgatg ccgtgcggaa tgtcgaagcc gaacacggtc aggatgccag
    24841 ccaagccccg caaaacaacg gcaaattcca gagaagtaac gaccgaattg accatgccgc
    24901 gcacaaattc gatggtcgaa gcgataaatt cctgcgcgtc ctgttggata cgctggtcga
    24961 tgttgtctgg cgcgtggcgg cgcatttgca ggcggtagta gtttttgtcg gcaagccagc
    25021 gtgctgtcag cacttcgttg agccgctccg accatttaat cgccaagcct tgatcgagga
    25081 agtcgttgac gacgttgtta aacgcccgta tcagcaccac gcccgcgttc attgaggcaa
    25141 acatccaaaa tgccgaagca ttcaaatcct gcatcgagtc gtaaagccct ttggacataa
    25201 aggtactcaa cacattcagc cgtacttcgg ttaacagcag cgtaaccatt gccgtaatca
    25261 gcagcaatac tttgaccgcg tttttcggtg tcagacaaag gcgcaggata taggcaaact
    25321 cccgcccgaa gcgcgttttc cgtgtcaaaa acagaatcac cgctgaagcg gcagaaacta
    25381 tcaagagagt ctgtaaaagc caaagcggtg tggaataaag ctcggtttgc catttttgca
    25441 tgggaaatct cttacggtat caatgccgtc tgaaaagacg ggtacagttg attttttgat
    25501 gaagtttggg gaagttttgc aggtcagggc atattgcgtg ttaatttacc atacaaccac
    25561 gccggaatta agtttaaatt tgaataaaag gttcgggttc tgtaaaatac agaacccgaa
    25621 ccttgttcgg atattgaaac cggccgcccg attttgggcg gtgcggcttg caagtatcaa
    25681 gattcgcata tgccgtctga agctcggaga ggttcagacg gcatatgctt atttgggctg
    25741 ctcttcgacg aatctcggac ctttcaagat gccgttgtga gaatagggcg acagcaggtt
    25801 gtatgcggcg gttttggaaa cctgataacc gcggtcggtc aggctgttgg caatctgatt
    25861 gaccactgcg ctgaccaaag cccccaacag gccgctgttg ctgttgttgc tgccttcgcg
    25921 gatgctggcc gaacccgacc acaactcttt tccgttgcgg gaatcgacca gccgtgcttt
    25981 ggcggatacg gtcgtcacgc tgtctaaaat ttgatatgaa gtgccgtatt cggtaaccgt
    26041 aatgtacaaa accgcatcat tgccgaaaat ctgatgcagt ttttccggcc ggacggcgtg
    26101 aatatcggcg gcattggtca agccgttttg tttgaaggtt tcctccacga ctgcggcggg
    26161 gaagacgtaa tagccggctt cggaaagcgg cgcggcggtc gaagccagta caccccatgt
    26221 tccgttgaca tcgggcgatt cgttcagcgg cggaaccacc aaaattgaag ccggtttgct
    26281 ttccttgaat gacgtgtagt cgaaatcggg cgctttttga acttggcagg cagacagcgc
    26341 caacacggcg gcaagcccta aaatcaaagg tttcatcgct tgcctccttt acctgttttc
    26401 atcaggaagt ccataaatac gcccgattcg ggaaacagcc ttttctcttc ttcaaactgg
    26461 cggaacgcgc cctctttgtc tcccgaacgg gaaagcagca gtcccagatg ggcgtgcgca
    26521 cccggggcgg cattcatttt tttgttgccg gcttccacaa agtatttttc catcttttcg
    26581 gtctgcttgc ccaacgaagt gttgtcgttt ttcaaacctt catagacggt atcgggatag
    26641 ccgccgtaat aatacaggga tttttgcccg ttgccgccgc aggcggtcag agccaagacc
    26701 gccgcacaca gcgacaaacg gctcaaggtt ttcggattca tcatttctcc ttaacggttg
    26761 ggttgccatg cgccgttgtc aacagcctga accagactgt tgacggcttc gcggattgcc
    26821 aagtctaaaa ctttgccgtt caaagtcgca tcgtagccgg aagtgccgcc gaaaccgatg
    26881 atttcacggt tggaaagtgc gtattcgccc gcgccctgtg cggaatagac gatttcggaa
    26941 gtattgacgt tgacgatatt cagagccact tttgcatagg cgatttgcga tttgccgcga
    27001 cccaaaatgc cgaagagctg atgatcgccg acatctctgc gtccgaattc ggttacatcg
    27061 ccggtaacga cataatctgc gcctttcagg ttatgcgctt tgccggaaat gccggattcc
    27121 tgttttaatg cgatcaaatt ggtgcggttc agtacgttga agcggttggt ctgttgcagg
    27181 tgcgttacca gaatggtttt tgcctggctg cccaaacggt cttccccgtc ggagaaaatg
    27241 cctttttgga agctggagcg gttgtcgaat gttccgacgg aaatcggggt acgaacaccg
    27301 tgatattgcg tattgtagga ggcgactttt gctacctcga gactgcgtga ggattcggtc
    27361 gcacagccgg tcagtgaaac ggcagcggcg gcaaggacaa cggcggtgga aacggttttc
    27421 ataaaattta ccctaaggtc aagttaagga aataacgggt tgtcattatt gtccttatgt
    27481 aaatttaagt caaggtgttt gtctgtgcgg gacggatgcg cacggaaggt acggatattt
    27541 ttcaaacgga atcgcggcgc gggcgggatg cctgcctttt ccggatttag aggtaaacga
    27601 tttcgccgct ccgccctttg ctttcggcac ttgcccacca gacaaatgcg ggcagcacgt
    27661 ccccgtagct tttgcgttcg cttttggctt cgcccggatg ggatttgatg cgttgcgggg
    27721 aattgatggg tccggggacg aggacgttgg cgcgcaggtt gccgaagcgt tcccattcgt
    27781 cggcggcgac tttgcacagg tagttcaacg cggctttgga cgcgccgaag ccgccccagt
    27841 aggctttggg ggtttccccg tggctttcgc cgacgaagat aacggatgcg tccggcgact
    27901 gtttcagcag gggaaggagg gcgcgggtca gccccatagg tgcgacggtg ttgatgcggt
    27961 attggttgac ccattcggcg acggtttgga aatccagcgg cgagagggcg taaaaatagc
    28021 cggcgcagtg gacgatgccg tccagtttgc cttgcgtggc ttcggcaatg gtggcggcga
    28081 actgattgaa ttctttttct tccgcactca tcaggtcgaa acggatggcg aagggttcgg
    28141 ggtgtccggc ttcgacaatc gcgtcataga ctttttccag ttttttctga tggcgtgcca
    28201 ccagaatcac ggttgcgcct gccgccgcat aggctttggc gacctgttcg cccagacctt
    28261 gcgatgcgcc ggtaactaag atggttttgt tggacagtgt cgccatactt tttccttttt
    28321 ggtggtcggt cgggggtatt ttagcgtttt gccgcacctt gtaaagcgtc ccgataccag
    28381 ccggcgtttt cagacggcat cgctcaggct ttgcaggcag gtttccgccg ccgcgaaacc
    28441 tgattgtacg gcggcttcga gcgtggcggg gtagtccggg tggaggtagt cgccggcggg
    28501 gaagatgcgg tgccggtgca gccacgacaa atccggcggc ggggcatcgg ctgcggttgt
    28561 ggcgcgtttt tcggtgatga cgcgcacggc ttcgggttcg cccaaatgcg gaaggatgcg
    28621 tttgaggtcg gcgtgaactt tgtccgccca cgctcggttt gtaaacgcgc cgacgcggtc
    28681 ggaaacgctg atgacggcgg acacttcgtt ttcaggcagt ccgagcctgc cccggcaaag
    28741 cagccattgc accgtgccgt cggcaaggcc ggtcagcggg gcgggcaggc ggacgggttc
    28801 ggcgtagcgc agatagacgg tggtgatggc gtggtagcga aggttttgat atgccgtctg
    28861 aacgtgttcg ggcgtgcctt cgggcaggag cgcggcggcg tggtagggcg cggtggcggg
    28921 gacggcggca tcgaaagctt cgccgttgac gagcactttc ccgtccggga gggtgttcag
    28981 acggcatacg cgcgtttcga ggcggatgtc cgcgccgagc cgttgaagat ccgccaaggc
    29041 gggttcggcg acgattgcgc ccaaatcctg cttgggtagg agatagtcgc tgccggattt
    29101 tttcgtcagc acgccgtcgg acaaaacgtt gcacaacacg cgcaggcttg cggtttccaa
    29161 aggcgtgttg agcgcgcccc aaaccaaggg ctgccaaaac tgcatcacgg cggcacgcgg
    29221 cacgttccgc tgtttcagcc attgcgccac tgtcgtgtcg ggctgtccga ggcgtgcgga
    29281 cttctgcaaa tcggacatat cggcaagcag tttggctttg aatgcagtcg gtgcacgccg
    29341 ggcaagcagc acgccgccca aaatatgcag cggcgcgggc agggggaggg cgcggaactg
    29401 caaaccgccg tgcatatgcc agtgcagcgg tacgcgcaaa aaggcggcac ggggatccga
    29461 accgatggtt ttcatcaggc gcaacacgcc ccggtatgcg ccgagcaaaa tgtgctgccc
    29521 gttgtccaaa aaaccgaaac cgtcggtatt tccggccagt gcgcgcgccc tgccgcccgc
    29581 ctgccggccg gcttcaaaca gggtaacgtc ggcgtgccgc gccaaggtga cggcggcgga
    29641 cagtcctgcc cagcctgcgc cgatgacggc gattttcggg cgcggatgcg gcgtgttcat
    29701 catttattcc tccaatggtt gaaaccccgt ttttcggggc ggcgggggtt gggttttcct
    29761 gaagggcggg cggtatgcgc cttatgcccg ttccggcgtg ccggggcgcg gtttgaatcc
    29821 gaataaccag gttttcaggg caatgcgttt tttgcgcggc gaagggaggg cgattttgta
    29881 tttgaggacg ttttgcgcgc cgtcccggtc gatttcgttc aatagctcgt aataaaccgc
    29941 cgccataacc agtccgactt tttgggcttt tttatcggca gccggcagca gcgatacggc
    30001 ttcgcggtag gtttcacggg cgcgtttgat ttggaacgcc atcaattcgg caaaattgcc
    30061 cgtcgggctg cattgcaaaa tcacgcttgc ggggacatca aaccgctgca tttcctccat
    30121 cggcaggtaa atccgtcccc tgcgcgcgtc ttcgccgaca tcgcggatga tgttggtcag
    30181 ttgcagcgca agtcccatct tgtcggcgta ttccagcgtt ttgccgtctg aaaaccccaa
    30241 aatccgcgca atcaggcagc cgactacacc tgcaacacgg cggcagtaca gtttcaattc
    30301 ttcaaaactg ccgtaacgcg cccgaaccaa atccatctgc atcccgtcga ttaaggcttc
    30361 cagttcatat ttcggcagct tgaaggtttc cttaacctgc cgcaaggcct gattgacggg
    30421 gtgttccggc atcgcgccgc cgaatacctt gtccaaatcg ccgcgccacc agttcaatgt
    30481 cgcctgtgca acatcggggt tggaacattc gtcaaccaca tcgtccaatt cgcggcaaaa
    30541 agcatataaa accgttaccg catcccgttt ttcctgagtc aggaaacgga agcccgacaa
    30601 aaaactggag cggctttctt ctgctttttg gcggcaatag tcgagtcctt tcacgattta
    30661 tattcctaat gatgggaggg aaaggcggat tttatcggca tttggcggta gagggcaatt
    30721 tcggcggcac gacccaatcc ttagcggttt gctcaactat cggcgcgaat tctgttaaaa
    30781 tgccgacttc cttcctttac acaccgcacc gacaggcaga atttatggct cttttgcaga
    30841 tttcagaacc gggtatgtcc gccgccccgc accgacaccg tttggcggca ggcatcgatt
    30901 tgggtacgac caacagcttg gtcgccaccg tccgcagcgg cagtgccgcc tgtctgcccg
    30961 atgccgaagg gcgcgttacc ctgccttccg tcgtccgcta tctggaaaac ggcggcattg
    31021 aagtcggcaa aaccgccctg tccgcccaaa aaaccgaccc gctgaacacc gtcagctccg
    31081 ccaaacgcct tatcgggcgg acgcttgccg atctgcatca aaatacgcat tacctgcctt
    31141 accgtttcgg tgacaatcaa cgcgttatcg aactgcatac gctgcagggg gtgaaaacgc
    31201 ctgtcgaagt gtcggcggaa atcctcaaaa cccttaaatc gcgcgccgaa gaaaccttgg
    31261 gcggcgattt ggtcggcgtg gtgattaccg tccctgccta tttcgatgat gcccaacgcc
    31321 aagccaccaa agatgccgcg cgtctggcgg gtttgaacgt attgcgcctg ctcaacgaac
    31381 ccaccgctgc cgcaatcgcc tacgggctgg acaacgcctc ggaaggcacg tttgtcgtgt
    31441 acgacttagg gggcggcaca ttcgacgtat ccgtattgca actgaccaaa ggactgtttg
    31501 aagtcaaagc caccggcggc aacagcgcgt tgggcggcga cgatttcgac caccgcctgt
    31561 tctgccgcct gctcgaacaa aacggactct cccaactcaa cgaacaagac agccaactcc
    31621 tgctctcgct cgtccgcgcc gccaaagaac aattaaccac gcaaaccgaa gcgcgcattc
    31681 aggcgacgct ttcagacggc atggcaatcg acacaagcat cagtcgcgcc gagttccaca
    31741 acctgacgca gcatttggtg atgaaaacgc tcgaaccggt cacacaggcg ttgaaagatg
    31801 ccggtgtcgg taaaaacgaa gtcaaaggcg tgattatggt cggcggttcg acccgtatgc
    31861 tgcacgtcca acaggcagtc gccaccttct tcggacaaac cccgctgaac aacctcaatc
    31921 ccgacgaagt cgtcgcgctc ggcgccgcca tacaggcaaa cgtcctcgca ggcaacaaaa
    31981 ccgacggcga atggctgctg ctggacgtta cacctttgtc gctcggtttg gaaacctacg
    32041 gcggcttggc ggaaaaaatc atcccgcgca attccaccat ccccaccgcg cgcgcgcagg
    32101 actttaccac cttcaaagac ggtcaaaccg cgatgacgat acacgtcgtg caaggcgagc
    32161 gcgagctcgt ttctgactgc cgcagccttg ccaaattcac cctgcgcggc attccaccga
    32221 tggcggcggg tgcggcgcgt atccgcgtaa ccttccaaat cgatgcggac gggctgctgt
    32281 ccgtttccgc ccaagaacaa agcaccggcg tacaggcgca aatcgaagtc aaaccttcct
    32341 acggcttgga cgacggcgcg attacccgaa tgcttaaaga cagtatggac aacgccgccg
    32401 aagatatggc ggcacgcgcc cgcgccgaag ccgtagtcga agccgaaagc ctgaccgatg
    32461 ccgtcaacgc cgccctcgag ttggacagcg atttactgga tgccaaagaa ttgcaacaga
    32521 tccggcaagg catcgccgat ttgcaaggcc gtctgaaaga cggaaaagcc gaagacatcc
    32581 gctccgccgt cgccaaactc agccgcagca ccgacaactt cgccgccaaa cgcatgaacc
    32641 gcaatatcca acgcgcgttg actggtcaaa gcgttgataa tatttgacgg caaatggttt
    32701 cagacggcat tggataaaga ggtcgtctga aaaaatatca tctcacagaa ataaaataaa
    32761 aaacaacaat ggaaaagtca ggcattgtct atctgatgaa aaccgtcatc aagggcgtgt
    32821 ataaaatcgg catttcggat gtaagcaatt ttgaaggcag aatgcgccat ttggaaaaca
    32881 acggttatgc gaacgttgcc gggttggaac gcatcctcgc cgtcaaaacc gacaattaca
    32941 aagaaaaaga aaacctgctc catgaaattt ttagtaaaag caggataggc gataccgaat
    33001 tgttcgccgt ggacgagaat cttgtaaaac gtttgttttt atcgcttcgc ggcgaaatcg
    33061 tgttcccgaa aaacgaaacg gcggaatcgg aatttgaaaa aagcgtccac gaacgcaggc
    33121 aggaagggaa tgccggatcc ggtcgcaaac aactgcttga tttggtacgg cgcggacacc
    33181 gggaataccc ttacgcgctg ccccggcttt tggcgggcgc ggcatcctac aagccgaaaa
    33241 aatcgaaaat ccgccttttt aaagaagcat atttacgcaa aagcggtacg aggctgaccg
    33301 acgaaattgc agacggcatc catatttaca cctgtttttc gcgggcggat ttggaaaaag
    33361 cctattccga atatttggaa cttttcaaat ccgaatcgga tgccgaaggc agaaaaccac
    33421 ggtaaggtgc aaacagatac cgtacacgtt gaggagcagt atgatgggcg attccgtcat
    33481 ttattatgta gaacaggcag acgaaccggt aaaccgtgcc ggcgaacgcg cccgtaaaac
    33541 attcaaatac ttttggcgcg agcttttttg ggaacgccgc cgcatcattc ccgccttaga
    33601 ttttgccatg gtcaaagccc cttttttcca agacggcgaa gacggcgaaa tttgcgaaca
    33661 tatgtggatc gacgatattt attttgacgg tctttacatt tacggtgtgc tgaacaataa
    33721 acccggcgaa ctgactaatg tcgaacaagg cgaaagcatt tgcgttccgg ttgacgacat
    33781 cagcgactgg atgttcgtgt gcaacggcat tccctacggc ggctttacca tacaggcaat
    33841 gcgtggacag atgacggaag aggagcgtac cgaacacgat gccgcatggg gaatcgattt
    33901 cggcgatccc gggcaggtat tgctggtgta tgaagaaaaa gaacatcccg aaaatctgga
    33961 agagcatccg atgtgccgga actgtattga cgattttcgg caacagttgt cccaaaatcc
    34021 ggattttctg catgagcagg acgaagacgg ctatacgccg cttcatcatg aagccatcgc
    34081 aggaaatgca cttatggttc aagccatgct tgaatacggc gcaaatcctg cctcaaagac
    34141 atcggaaggc tataccgccc tcgattttgc ccgcctgacg ggctggcaaa atgttgccga
    34201 cctgctcgaa ccgcgacatt aggcagacag ttttccgaaa acgaacacaa acacttttta
    34261 cagaaagaca ataaaaatgc ccaaaatcac cgtacttcca cacacgacat tatgccccga
    34321 gggtgcagtc atcgataacg cacccgaagg taaaaccgtc cttgacgtgc tgctcgacca
    34381 tgatatcgaa gtcgatcacg cctgcgaaaa atcctgcgcc tgcacaacct gccacgtgat
    34441 tatccgcaaa ggtttcgaca gtctggaaga gccgaccgaa ttggaagaag acctgctcga
    34501 tcaggcttgg ggtttggaag ccgattcgcg cctgagttgt caggcggttg tcgccggcga
    34561 agatttgatt gtggaaatcc ccaaatacac catcaaccac gcgcgcgaag aacactgaaa
    34621 acaggccgtc tgaagccggc acgcttcaga cggcattgtt gcgcggataa ggcgcaatcg
    34681 cccgaaaaca ggcgttcgta caggcggaac tttcgattct atagcgaatt aacaaaaatc
    34741 aggacaaggc ggcgagccgc agacagtaca aatagtacgg caaggcgagg caacgctgta
    34801 ccggtttaaa tttaattcac tatatattga tttttatcgg ttttctgacg gaacggattg
    34861 gtgcggcatc cgaggcggat tactcgggcg cgatgcacgg gtatttatcg gttttgcagc
    34921 cggaaaaacc gccggcgggt tactttcagg gcggcggtcg ggcggtatgc cggatagcgt
    34981 tccactttcg cttctatatt gattttgatt ggttttctga cggaatgacc cgatgcggca
    35041 tccgggacgg cttgtgtttt ttcctgcccg cctgccggat tttcccatcc ttgcgtgaaa
    35101 ccgaaagaga cggcggcgcg gcggacaagc tcgagatagc gtccttcaag ctccgggcag
    35161 gcggcggaca cgctttctgc cgtaaccgtg aagccgccgc ccgacccgga aaggcgttcc
    35221 gcaatctgcg tgtagatacg ccaacgttgg gcgcgcaaca ttgccgcatc gcaaccggca
    35281 gccgctttat ccaaggcaat caggccgcgc cggttttggt ggtgaaggca aaccgttact
    35341 ctggagagga tatgttgccc gtccaatatt tgatacagtt tgcgtatcag cagaatcagg
    35401 cggtcaaact cctccatctc cgacagggaa tcaggatggt cggaagcggt ataaaccagt
    35461 ttggacaatt ttgcggcaaa catattgttc atcaatcttc cttgtcggtt gaaaccccgc
    35521 tcttcggggc ggtagaatca gatttgtttg ggagggacaa ctcctcccag atcaggacga
    35581 cacataggct ggtgcttgat gtgttgtccg gcgagttgaa acattcagcc atcctcaagg
    35641 tgcggcagtt ttgccgaaac atattctaca cggcttcaat gccggacgat aaaaggaaat
    35701 tcatatgaaa tggaccgaca cccggcgtat cgccgaagaa ctctatgacc tgcacggcga
    35761 aaccatcgat cccagaaccg tgcgctttac ccaactgcgc gacctgatta tggcattgcc
    35821 cgaatttgac gacgaccccg cccgttgcgg cgaacgcatc ctcgaagccg tgcagcaggc
    35881 atggatagac gaggcggaat aagtttcggg aatgccgtct gaaatgcggc ggtacgcggt
    35941 tcgtgcttct gtttgcagcg ggagtggttt taccagtctc cttttttcag cctgtccagt
    36001 tggcggcggt cgcgcttggt cggtctgccg tcgggatagg cggaagtgat gcggctgaat
    36061 tggtcgagct gtttgcgctc ttccctcaat gttgccgttt tcgcgtcctc ttcatacaga
    36121 agccgcgcct cggatgccgg gcggcgttgg tggttcaaac ctttaacctt gattttatag
    36181 ggaagggaat tgagcgtcag gtcgataata tcgccgatgt ctatggtttt actgtttttg
    36241 accttcgagc cgtttacttg aaccctaccc agttcgatgt gcttttgcgc aagggaacgg
    36301 gtcttgaaaa aacgtgccgc ccaaagccat ttgtccagcc gcatggcgga agaatcgtgc
    36361 ttgtctttca tacgattttg tttgaaataa ttgaatttgt ttcaagttta gcataagata
    36421 cgccgcctat aactagtata tatgcactaa tccactgttt tccatgctgt ccgaacacaa
    36481 aaagagggtg tggaaaagcc gttttggaca ataaattaac tgcggaatat gcacaaatag
    36541 cgtatgatag cggcagaatc tgttgatgag agcttcattc tatgaaacct gtttttttgg
    36601 attttgaaca acccatagcc gaactgacca acaaaatcga tgagctgcgt ttcgtccaag
    36661 acgagtctgc cgtcgatatt tcggacgaaa tacaccgttt gcagaaaaaa agcaacgacc
    36721 tgaccaaatc gattttcagc aaacttacgc ccgcccaggt ttcacaggtt tcccggcatc
    36781 cgcagcgtcc ctatactttg gattacattg aggcactgtt tactgatttt gaagagctgc
    36841 acggcgaccg ccactttgcc gacgattatg cgattgtcgg cggattggcg cgtttcaacg
    36901 gacaaagcgt gatggtcgtc gggcatcaga aagggcgcga caccaaagaa aaaatccgcc
    36961 gcaacttcgg tatgccccgt cctgaaggct accgcaaagc cctgcgcctg atgaagacgg
    37021 cagaaaaatt cggcttgccc gtcatgacct ttatcgatac gccgggcgcg tatcccggca
    37081 tcggcgcgga agaacgcgga cagtcggaag ccatcggcaa aaacctgtac gaactgacgc
    37141 gcctgcacgt ccccgttttg tgtaccgtca tcggcgaagg gggctcgggc ggtgcgttgg
    37201 cggtcgccgt aggcgattac gtcaatatgc tgcaatattc gacctattct gttatctccc
    37261 ccgaaggctg cgcttcgatt ttgtggaaaa ccgccgaaaa ggcggcggat gcggctcagg
    37321 ctttgggcat tactgctgac cgcctgcaaa agctggactt ggtcgatacc gtcatcaaag
    37381 aaccattggg cggcgcgcat cgggatttcg ggcaaaccat gaaaaacgta aaagccgttt
    37441 tggaaaaaca actgcacgaa gcgcaaagca tcccgcttgc cgatttgctt tcgcgccgtt
    37501 tcgaccgcat tatggcttac ggcaaatttt cggaacaata attcaggtag aacaagcagc
    37561 aagcagtttg tctgaaactg cttgcttttt ctttatcggg acggaaccgt gctgacttta
    37621 gatgcgtttg agcaatgctt gaaggattgt tttcctcaag gtctgaatgg aaaaaaaaca
    37681 gcggtggcat taagcggcgg cttggattcc gtcgttttgc tgcatctgct tgtccgcgcc
    37741 gggaaaaaag gcggttttat tccggatgca ttgcatatcc atcacggctt gagtccccgt
    37801 gccgacgatt gggcggattt ctgccaaaac tattgcgata tgctcggggt ggggctggaa
    37861 acggttaaga tctgcgtgga aaaaaacggt ttgggcatcg aggcggcggc gcggcaaaag
    37921 cgttatgccg cgtttgccga aaaaggcttt gacgttttgg cgttggcgca ccacagggac
    37981 gatcaaatcg aaacctttat gctggcggtc gcgcgcggcg gcggtttgcg cgctttggcg
    38041 gctatgcccg ccgtccgccc ttttggggaa aaaggcatca tttggcggcc cttgctgcct
    38101 ttttcgcgcc aagatatatg ggattatgcc caaaaacacg gtttgccgaa tatcgaggac
    38161 gaaagcaata gcgatacggc ttatttgcga aaccgcttcc ggcaccgtat tttgcccgaa
    38221 ctttcggcgc agattcccca tttcgggcgg catctgttga acaatgtccg tgccttgcag
    38281 gaggatttgg ctttgttgga cgaggtcgtc gttcaagact gccgttgggt ttgcggagcc
    38341 ggttatttcg atacggcgcg gtggctgacg ttttccccgc gccggaaaac ccatattttg
    38401 cggcattttt tgaaggaaaa cggcattccc gtgccgaatc agaatgccct tgccgacatt
    38461 gcccgggttt tgacggaggc aaaaaccgga cgttggaacg tgcaaggctt tgaattgcat
    38521 cattatgcag gcaggctgtt tgtgttccga ccggaaaaaa cggataaact gcggtttttg
    38581 aaagacaggc agataagcgg aaatttaagg gaaatattgg cggggcaggg atttgtttta
    38641 aaacgtcatc cgttcggact tcccgggcat cttttggagc aggacggaat cttgcggacg
    38701 gttgcggcat cggacaggct ggctgtcggc ggtatccata aggatgtgaa gaaaatcctt
    38761 caggaaaagc gggttttgcc cgcgctgcgc ccgttttggc cggtcgtcgc cgacaaggac
    38821 aaccgtccgc tggcgttggc aaattacggt acggattttc aaatctcggt ttcagacggc
    38881 attttgcccg tccatccgga ttttcctgtt ttattttgat ggaatcggaa tcgccgttac
    38941 ctattatata ttcttgaaaa ggcggtaaag tgaagcatat cagttcggca aacaatgaac
    39001 acatcagaca cctgcaccgc ctgttgtctc aagggaagtt cagacggcaa tacgcccaaa
    39061 ccgttttgga gggcgtgcac ctgcttcagg ttttcctgca atccggcgga aggccagtcg
    39121 gggtatatat tcccgaagcg aaaatgccgt ctgaagaagt ccgtaaattg acggcggttt
    39181 tgccggaaga cggggttttt tccgtttcag acggcatatt gaagaaaatc agcagcctga
    39241 cttgcgcgga tgatgtgctt gcgctgattg atattccgga tgcgggtgct ttgccggccg
    39301 gcggcgactg tgtggttttg gacggcgtgc aagatccggg caatgtcggc acggtgttgc
    39361 ggagcgcggc ggcggcagga atcggcgcgg tcgttttggg caaaggttgt gcggacgcgt
    39421 ggtcgcccaa agtgctgcga gccggaatgg gcgcgcattt cttgtcggag atttatccgc
    39481 aggcggattt ggaaatatgg ttggcgcgtt atgaagaccg tgtgtttgcc accgcgttgc
    39541 gtgaggaaaa acaggcggtt ctgtataacg aagatttgcg cgaaccgaca gcctgggtgt
    39601 ttggcaacga aggcgcgggg gtcggtaaag cagttttaga cagggcggac aagtgtgtca
    39661 ggataccgat gcacgatgca accgagtctt taaatgtcgc gatggcggcg acaatctgcc
    39721 tgtttgaaca aatgcgccaa cgggcggcgt attgaggaaa agaaatgccg tctgaaaaaa
    39781 tctattacgg cgtattgatt ttcttatgta tcgcttctat gctgctgtcg ccgttttttt
    39841 atgcgggtgc tttgaagccc aagaaggcgg cattgcggaa ggacgggcag tggaaactca
    39901 tcttattgtc caataccgtg gcggcggcgg ttttggcttg ggtgtggtgg aaatggtttt
    39961 gacagatatt gctcaaaatc gtgctaatgg aatccgaaca aataaagaat ttggtaaaaa
    40021 atttgttaaa tcaacgaatt aaagttttgt ggaaaacaaa acagctctaa gcaaataggg
    40081 cgtttgtcgg taaatacgga agagttgcgg cattatcggg catctttaac aagtagtgcc
    40141 gtcttgacag gcaatcggtt tttatgggca gcttgcaaaa tcgcggatat aaaattgcaa
    40201 atcggttaaa gtgtggggac gctatgaaaa attgcgaatt tttttatgac ccgacaaggg
    40261 caatctatga tagcggtgca gattatttaa ccagagaaaa acatagattc gtcgtaattg
    40321 caaatagtgc ttgggggcta ttgcttaatt tatcttgtta ttatgacgag gttttggaaa
    40381 agcggaaaat accgttcggc aaacaggaaa ttgatgacga tatggacaaa gtgtccgccc
    40441 ataagcggaa gtttaaagat atttctgaaa tcaaagtagg ggatggttgg gaatacccgt
    40501 tcaattatga gcagggaatg aaagaattag atgaagtgct attgaaatac attccctttt
    40561 ttgaagaaga acgataaagg aggttgatat gcgcgtatct aaaataattg gaagtatgtt
    40621 gcttgttaca gcggttcaga ccgtattttc ggcaaatgtt tacgcgtgcc gccataatgg
    40681 taaaaccagt tacagccaaa ctccgggaaa acattgtacc aacgcgggtt tggggggcgg
    40741 gaccgggtgt acagttcggt tagaccggca gtaaaagaca gggcggaaga cgcaggagtt
    40801 ggcgattatt cggacacggt gagggacgaa cacgtccaaa ttccgaaagg gaatgcacag
    40861 aaagacggtt cggatgccgg catcaggccg cattgattga aaccgaatca accatgacgg
    40921 tgttgaacaa tagaatttga acgattaggg aactctgatg aaacacatcc acattatcgg
    40981 tatcggcggc acgtttatgg gcggagtggc cgccattgcc aaagaagcgg ggtttaaagt
    41041 cagcggttgc gacgcgaaga tgtatccgcc gatgagcacc cagctcgaag ccttgggcat
    41101 aggcgtacac gaaggcttcg acgcggcgca gttggaggaa tttaaagccg acgtttacgt
    41161 catcggcaat gtcgccaagc gcgggatgga tgtggttgag gcgattttga accgcgggct
    41221 gccttatatt tccggtccgc aatggctgtc ggaaaacgtg ctgcgccatc attgggtact
    41281 cggtgtggcg gggacgcacg gcaaaacgac caccgcgtcc atgctcgcat gggttttgga
    41341 atatgccgga ctcgcgccgg gcttccttat cggcggcgta ccggaaaact ttagcgtttc
    41401 cgcccgcctg ccgcaaacgc cgcgtcaaga cccgaacagc aaatcgccgt ttttcgtcat
    41461 cgaagccgac gaatacgaca ccgccttttt cgacaaacgc tccaaattcg tgcattaccg
    41521 tccacgtacc gccgtgttga acaatctgga attcgaccat gccgacatct ttgccgattt
    41581 gggcgcgata cagacccagt tccatcacct cgtgcgcacc gtgccgtctg aaggcttaat
    41641 catctgtaac ggacagcagc aaagcttgca ggatacttta gacaaaggct gctggacgcc
    41701 ggtggagaaa ttcggcacgg aacacggctg gcaggccggc gaagccaatg ccgacggctc
    41761 gttcgacgtg ttactagacg gcaaaaccgc cggacgcgtc aaatgggatt tgatgggcag
    41821 gcacaaccgc atgaacgcgc tcgccgtcat tgccgccgcg cgtcatgtcg gtgtcgatat
    41881 tcagaccgcc tgcgaagcct tgggcgcgtt taaaaacgtc aaacgccgca tggaaatcaa
    41941 aggcacggca aacggcatta ccgtttacga cgactttgcc caccatccga ccgccatcga
    42001 aaccacgatt caaggtttgc gccaacgtat cggcggcgcg cgtatcctcg ccgtcctcga
    42061 gccgcgttcc aacacgatga agctgggcac gatgaagtcc gccctgcccg aaagcctcaa
    42121 agaagccgac caagtgttct gctacgccgg tggcgtggac tggaacgtcg ccgaagccct
    42181 cgcgcctttg ggcggcaggc tgcacgtcgg caaagacttc gatgccttcg ttgccgaaat
    42241 cgtgaaaaac gccgaagcag gcgaccatat tttggtgatg agcaacggcg gtttcggcgg
    42301 aatacacacc aaactgctgg acgctttgag atagcccggg cgatgccgtc tgaaagccct
    42361 tcagacggca tc
//
LOCUS       DAWFLT010000009        40902 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWFLT010000009 DAWFLT010000000
VERSION     DAWFLT010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40902
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.009"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(171..863)
                     /locus_tag="WGR74_000433"
     CDS             complement(171..863)
                     /locus_tag="WGR74_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0904920.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGI
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(971..2164)
                     /locus_tag="WGR74_000434"
     CDS             complement(971..2164)
                     /locus_tag="WGR74_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0904921.1"
                     /translation="MSNQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2293..3030)
                     /gene="pxpA"
                     /locus_tag="WGR74_000435"
     CDS             complement(2293..3030)
                     /gene="pxpA"
                     /locus_tag="WGR74_000435"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ0904922.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGQVKAVDGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3017..3946)
                     /locus_tag="WGR74_000436"
     CDS             complement(3017..3946)
                     /locus_tag="WGR74_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ0904923.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDYLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(3943..4590)
                     /gene="pxpB"
                     /locus_tag="WGR74_000437"
     CDS             complement(3943..4590)
                     /gene="pxpB"
                     /locus_tag="WGR74_000437"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ0904924.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4774..6981
                     /gene="uvrD"
                     /locus_tag="WGR74_000438"
     CDS             4774..6981
                     /gene="uvrD"
                     /locus_tag="WGR74_000438"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ0904925.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPVDVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     NAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTIENLQTASNEQGITLWQAACNAGAK
                     ATKVAAFVRLIEALRNQVGQMPLPEIIVGILKDSGLTEHYKTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFEILPENISDDPAFPILAFLSNAALESGENQAGADEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
     gene            7216..7674
                     /locus_tag="WGR74_000439"
     CDS             7216..7674
                     /locus_tag="WGR74_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0904926.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWLEEWEYPPIGVYFANDPSGGHALFALDYRECGRDGEPKVVFVEQESDF"
     gene            complement(7945..8160)
                     /locus_tag="WGR74_000440"
     CDS             complement(7945..8160)
                     /locus_tag="WGR74_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904927.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNPQSVQELEQHLIEAYDLMDTTG"
     gene            complement(8168..8371)
                     /locus_tag="WGR74_000441"
     CDS             complement(8168..8371)
                     /locus_tag="WGR74_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904928.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(8284..8643)
                     /locus_tag="WGR74_000442"
     CDS             complement(8284..8643)
                     /locus_tag="WGR74_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0904929.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            8696..9043
                     /locus_tag="WGR74_000443"
     CDS             8696..9043
                     /locus_tag="WGR74_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0904930.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWNRAKRHLRKFNGIPKAHFELYLKECEW
                     RFNNSEIKVLVPF"
     gene            9271..9891
                     /locus_tag="WGR74_000444"
                     /pseudo
     CDS             9271..9891
                     /locus_tag="WGR74_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
     gene            9914..11365
                     /locus_tag="WGR74_000445"
     CDS             9914..11365
                     /locus_tag="WGR74_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEZ0904931.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     IGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKFGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            11827..12183
                     /locus_tag="WGR74_000446"
     CDS             11827..12183
                     /locus_tag="WGR74_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ0904932.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            12174..12656
                     /locus_tag="WGR74_000447"
     CDS             12174..12656
                     /locus_tag="WGR74_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ0904933.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            12669..13262
                     /locus_tag="WGR74_000448"
     CDS             12669..13262
                     /locus_tag="WGR74_000448"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ0904934.1"
                     /translation="MASIQNLYETVSRVLGNQAGKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            13252..14508
                     /gene="nuoD"
                     /locus_tag="WGR74_000449"
     CDS             13252..14508
                     /gene="nuoD"
                     /locus_tag="WGR74_000449"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ0904935.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYEGSKFRNAKVLKQLNESREGTMLDFIDVFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKKQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            14508..14981
                     /gene="nuoE"
                     /locus_tag="WGR74_000450"
     CDS             14508..14981
                     /gene="nuoE"
                     /locus_tag="WGR74_000450"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ0904936.1"
                     /translation="MLSAESLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFIADYIGISPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQR
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            15371..16672
                     /gene="nuoF"
                     /locus_tag="WGR74_000451"
     CDS             15371..16672
                     /gene="nuoF"
                     /locus_tag="WGR74_000451"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ0904937.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIVRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            16672..17109
                     /locus_tag="WGR74_000452"
     CDS             16672..17109
                     /locus_tag="WGR74_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904938.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICWKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            17399..19660
                     /locus_tag="WGR74_000453"
     CDS             17399..19660
                     /locus_tag="WGR74_000453"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ0904939.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAMGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMANRGEHSEIMPFIGKAVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGIKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELFMPLLSQEAAHPDEWADRLKKLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLNVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPLWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWTGEGVQTASDRLVRVGGVGIYHTDAIVRRSAPLQATVH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLSLHTENAA
                     LGALMDTIELAGA"
     gene            19663..20739
                     /gene="nuoH"
                     /locus_tag="WGR74_000454"
     CDS             19663..20739
                     /gene="nuoH"
                     /locus_tag="WGR74_000454"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ0904940.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILISALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVILGLWMISPLNLWK"
     gene            20819..21298
                     /gene="nuoI"
                     /locus_tag="WGR74_000455"
     CDS             20819..21298
                     /gene="nuoI"
                     /locus_tag="WGR74_000455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ0904941.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            21329..22036
                     /locus_tag="WGR74_000456"
     CDS             21329..22036
                     /locus_tag="WGR74_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904942.1"
                     /translation="MEGLINALKYLAEHEPIDNFEEIRTRNSPIELPSGLSNFEQNIF
                     LKENLSPKLQNDDSLKTHYWIIREWGGIKSFKQSAENSQLIRQFLSELNSGKLSSGLL
                     KISSLSKLASFIDCERFAIYDSRAIFSLNWLLFKFTNADLFFQPQGRNRELEIRNMNV
                     LFHFSDIKPNYRKPDVSFHQYCGLLQDLAKQVYGKQAKPYHIEMLLFKIATTWICADM
                     DQLIKFDCLRNQDFQTA"
     gene            22072..22743
                     /locus_tag="WGR74_000457"
     CDS             22072..22743
                     /locus_tag="WGR74_000457"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ0904943.1"
                     /translation="MTFQLILFYIFAVIILYGAIKTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTVNPKRMDPADQVKVRADQGRMRLVKMEAVKPQTESAEESEVSDDLKPK
                     EEGKA"
     gene            22740..23045
                     /gene="nuoK"
                     /locus_tag="WGR74_000458"
     CDS             22740..23045
                     /gene="nuoK"
                     /locus_tag="WGR74_000458"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ0904944.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            23079..23654
                     /locus_tag="WGR74_000459"
     CDS             23079..23654
                     /locus_tag="WGR74_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine monophosphate-protein transferase
                     NmFic"
                     /protein_id="HEZ0904945.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            23778..24119
                     /locus_tag="WGR74_000460"
     CDS             23778..24119
                     /locus_tag="WGR74_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904946.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVSPK"
     gene            24168..26192
                     /gene="nuoL"
                     /locus_tag="WGR74_000461"
     CDS             24168..26192
                     /gene="nuoL"
                     /locus_tag="WGR74_000461"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ0904947.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHSLGKNDNPHESPLVVTLPLILLAIPSVIIGYVAI
                     EPMLYGDFFKDVIFVNTDAHPTMHIMKEEFHGALAMVSHSLTSPVLYLAAAGVAAAWL
                     LYVKLPHLPAKIAQTFRPIYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            26260..26580
                     /locus_tag="WGR74_000462"
     CDS             26260..26580
                     /locus_tag="WGR74_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEZ0904948.1"
                     /translation="MSNTQTIRSADFTASRAWGALDIANMNGTTVRLHWTDQSYKWHV
                     NDGEEVFAVMNGEVDMHYRENGEEHIVRLKSGDIFYAGIGTEHVAHPCGEARILVIEK
                     EGSA"
     gene            26639..27802
                     /locus_tag="WGR74_000463"
     CDS             26639..27802
                     /locus_tag="WGR74_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ0904949.1"
                     /translation="MTQLFLHSDTPNEKELISIYEKAVSEAVELYIFSAYLTHWDVKN
                     KLNSKLQEFKFIFGQDFGLSKKEAIQEVLKWLPSHLKFTLMVAQGIQGFHPKAVFWKN
                     DKNEYYALIGSSNLTHAAFNSNYEANILTKISEQDFIKAKSWANEIVMKSIPVSEDWL
                     EEYQEAEINYKKSTVRQSVMDKLFMEMPNYNQELIAARRKQMRNHQTVCNQLKNLIKQ
                     CAAGKIDNNDFYGEFNKLWSWKSENKGEGVGNRFQDKTWKRTGKSSDFRKLCIAIQSV
                     FDAPLTERDNVVAKQIDWLKECGVSTRGSVFSEMLCQEYPDRYPVLNAPIKKFLEENK
                     FKSAKGASEGSKYIDLSMKLRALLAMQSEIKDLAELDVLVQAEYRNRTDIDWE"
     gene            27916..29412
                     /locus_tag="WGR74_000464"
     CDS             27916..29412
                     /locus_tag="WGR74_000464"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ0904950.1"
                     /translation="MFSNYLLSLAIWIPIVAGVLVLATGPDSRAPFARVLALIGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQLPLGVQQLLFAAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     QDINCREFAILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            29422..30867
                     /gene="nuoN"
                     /locus_tag="WGR74_000465"
     CDS             29422..30867
                     /gene="nuoN"
                     /locus_tag="WGR74_000465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ0904951.1"
                     /translation="MNWSDLNLMPALPEVALLSLLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYARPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSSFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGHVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARPTVGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            30979..31278
                     /locus_tag="WGR74_000466"
     CDS             30979..31278
                     /locus_tag="WGR74_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ0904952.1"
                     /translation="MTASMYILLVLALIFANVPFLTTRLFGVAALKRKHFGHHLIELA
                     AGFALTAVLAYILESRVGAVHNQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(31387..32283)
                     /locus_tag="WGR74_000467"
     CDS             complement(31387..32283)
                     /locus_tag="WGR74_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ0904953.1"
                     /translation="MNPTNDLKAWQLRAQAQTELLLERFLPSENEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHAYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEANALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTAILGKTAGKDADNDKPTYVRLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(32267..32491)
                     /locus_tag="WGR74_000468"
     CDS             complement(32267..32491)
                     /locus_tag="WGR74_000468"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ0904954.1"
                     /translation="MKKNAPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGPKELNLESDE"
     gene            complement(32489..32578)
                     /locus_tag="WGR74_000469"
                     /pseudo
     CDS             complement(32489..32578)
                     /locus_tag="WGR74_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
     gene            complement(32605..33528)
                     /gene="rsgA"
                     /locus_tag="WGR74_000470"
     CDS             complement(32605..33528)
                     /gene="rsgA"
                     /locus_tag="WGR74_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ0904955.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETHALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETRAASPERLAFLQGITDELPG"
     gene            complement(33533..35389)
                     /locus_tag="WGR74_000471"
     CDS             complement(33533..35389)
                     /locus_tag="WGR74_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0904956.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMTGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPTELFAEKGWELAAMAAMMVFSVAWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPNALPLKVPQGAIKFEHVDFSYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGITQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            complement(35415..35552)
                     /locus_tag="WGR74_000472"
     CDS             complement(35415..35552)
                     /locus_tag="WGR74_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0904957.1"
                     /translation="MPSEGGAAFALTKPCVSAPRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            complement(35552..36136)
                     /gene="ruvA"
                     /locus_tag="WGR74_000473"
     CDS             complement(35552..36136)
                     /gene="ruvA"
                     /locus_tag="WGR74_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ0904958.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVSGIGAKTALGILSAMTADELAQAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            complement(36218..36544)
                     /locus_tag="WGR74_000474"
     CDS             complement(36218..36544)
                     /locus_tag="WGR74_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0904959.1"
                     /translation="MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHNKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(36598..37317)
                     /locus_tag="WGR74_000475"
     CDS             complement(36598..37317)
                     /locus_tag="WGR74_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ0904960.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVGQGTAHVKIEQIVPGQSTPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAASTSSPNLAIEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMIQTVLTAG"
     gene            complement(37314..37436)
                     /locus_tag="WGR74_000476"
     CDS             complement(37314..37436)
                     /locus_tag="WGR74_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ0904961.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            complement(37447..37911)
                     /gene="trmL"
                     /locus_tag="WGR74_000477"
     CDS             complement(37447..37911)
                     /gene="trmL"
                     /locus_tag="WGR74_000477"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ0904962.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVYENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            complement(37976..38473)
                     /locus_tag="WGR74_000478"
     CDS             complement(37976..38473)
                     /locus_tag="WGR74_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ0904963.1"
                     /translation="MWASLHYEPPVSNMIHALKHLADLSMVQPLADLMMQNPPDRLTD
                     ECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKG
                     GERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELAKTLKKSGANRICCWTLAR
                     TPMKK"
     gene            38529..39443
                     /gene="bioH"
                     /locus_tag="WGR74_000479"
     CDS             38529..39443
                     /gene="bioH"
                     /locus_tag="WGR74_000479"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ0904964.1"
                     /translation="MCLKHRGQLFAASVRYAAKSAAQPAQMPSETPDEPWHNTQRLIV
                     GASAMRRQQERKSMPDAVKKVYLIHGWGANRHVFDDLMPRLPATWPVSAVDLPGHGDA
                     PFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARL
                     TAAEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTHDADRIIGRILPDLARC
                     GTPSALQEALDAAERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLV
                     VMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            39445..40233
                     /locus_tag="WGR74_000480"
     CDS             39445..40233
                     /locus_tag="WGR74_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ0904965.1"
                     /translation="MNPQDKCWQVHRHLAEHADQRLTLVRNAPKHILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAVRKGGFWQRFTGKGVVQHCQSPTAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALETDGLLFFTCFGRDTLAELKCRLKENGIESRSA
                     LFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMSWNDENAA
                     RSCVGTIFEREGGLSITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            40510..>40902
                     /locus_tag="WGR74_000481"
     CDS             40510..>40902
                     /locus_tag="WGR74_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HEZ0904966.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLYDSGLTKTSTAL"
BASE COUNT         9487 a        10261 c        10943 g        10211 t
ORIGIN      
        1 tttcagttac agcagcttga tccctttccc ttatccaacg ggggaaggct aggatagggt
       61 ggcttgcaaa tatacagaac aagggacaag agccaccctc tctccaaccc tctccctccg
      121 tacgggaggg ggtggattct cgcgggcgaa gcccacgcta cggttagcct ttaccccagc
      181 acaaacaatt cccgcccgtg cgccttcagc caacttttag cattgtcggt atgcggcgtc
      241 agcgtgttca ccaaatgcca aaagcgcgga ctgtggtcgg ggtggcggag gtggcagagt
      301 tcgtggatgc agacatagtc ggcgacgtat tcgggcgtgc cgatcagccg ccagttgagg
      361 cggatgccgg tgtgcgggcg gcatatgccc caaaaggttt tggcgttgct caggtctgtg
      421 gcggtgggcg tcagtcctgt ttcggctgcg tgtttttcaa ggcggggcag caggtattcg
      481 cgggcgcgtt cgttcaacag gcggcgcagg tggtcgattt gtgcggcggt ttcttttcgg
      541 ggaagcagga tttcagacga cgtgatacgg atatggcttt ggctgtgggt atccagcttg
      601 gtctttattc cccgatacca aatccactcg ggtaagtttg ggtgggaaac aggatgcacg
      661 ggcgttttgg caagcgtgtt ccgcaaaatc gtttcgtttg ccgccagcca gtttgctaac
      721 gcgtggtctt gaaaaaaggg tgggacgttg atgctgaccg tctgcatatt gacggggcgc
      781 agaatcagat ttttcttggc actgcgtttg agttcgattt cgatgcacaa accgtcggaa
      841 agagtatagg tgaagcgttt catagttgtg aatgggtttc agaccggata catcgtctga
      901 aacaggaatt ttccatatca ggcggcaaac ttcggataat atacaaaatc aaacatctgc
      961 gctacaaggt tcagccgaac aagccgccga tatatttgct gatggtgatg gcgctgagta
     1021 ctgccatcaa accgaccaca atcacgccgg aaacggtgag ccacagcggg tgtttgtagt
     1081 cgccgacaat tttggttttg taggcggcaa tcagaatcag accgagggaa atcggtaaaa
     1141 tcaggccgtt taatgcgcct acgaacacca gcacctgcgc cggtttgccg atggtggaaa
     1201 atacggcggt ggacacggcg ataaaggcaa taatccattt gtttttattg cgttcgatag
     1261 acgggctgag accggagaag aacgacaccg aagtataagc cgcaccaatc accgaagtaa
     1321 tcgaagccgc ccaaatcacc acgccgaaaa tcagcaggcc gatgtatccc gccgcatatt
     1381 caaacggtgt ggaagcaggg ttgtcgggat tgagctgtac gccttggctg accacgccca
     1441 aaaccgccaa aaacaataca atccgcataa tcgaggcaat caggatcgcc cgcaccgagc
     1501 tttggctcac ttccggcaac gccgatttgc ctttgatgcc tgcgtccagc agacggtgcg
     1561 caccggcgaa ggtgatgtag ccgccgaccg tgccgcccac cagtgtaaca atcgccattg
     1621 catcgagttt ttccggcata aaggtatgca cggcggcatc tgccagcggc ggattcgcct
     1681 gccatgccac ataaaccgtc agcgcaatca ttacgaaacc catcacttgg gcgaatttgt
     1741 ccatcacttt gcctgcttct ttaaacagaa acacaccgat ggcaatcacg ccgctgatca
     1801 cggcaccggt ttccggcgac agtccggtca gcaggttcag acccaagcct gcgccgccga
     1861 cgttgccaat attgaacgcc aaaccgccca tcacaatcag cacagccaag aaatagcctg
     1921 cgccgggcaa gacctgattg gcaatatcct gcgcctgttt ttcggaaacg gcgacaatcc
     1981 gccaaatatt gagctgcgcc ccgatgtcga gcagaatcga gagcagaatc acaaagccga
     2041 aacttgccgc cagtgcttgg gtgaaggtgg cggtttgggt cagaaagccc gggccgatgg
     2101 cggaagtcgc catcaggaat gcagcgccga ttaaggcatt tctgcggttt ttttgattag
     2161 acataatcgc ttatcctcta taaaattggt tgttgctgtg tttgggcgaa acctgcggtt
     2221 ttagctacgc agaaactcgc tttgctcgtt ttggcgaaac ctgcggtttt cagacggcct
     2281 atgaactgtt tttcaagcag aaactttgat gcctgccgcc agtagttcct gccggatttt
     2341 ttcggcaaac accacggcgt gcggcccgtc tccgtgcaga cagatgctgt cggcttgcac
     2401 ggcaaccagg ctgccgtcca ctgctttgac ctgcccgtcc cgcaccatct gcaatacttg
     2461 ggcgatggct tcttcgtcgc tgtccacctg cgcatcgggg cggctgcggg gaaccagcgt
     2521 accgtcgggc atatagcggc ggtcggcgaa tacttcggaa atcacaccca agcctgcggc
     2581 ttttccggct tccaagagca ggctgccgga aagtgccatc aatttcaatt tcgggtcgaa
     2641 atccgccaca attcgggcaa cggtatccgc cagcgcacgg tttttcgccg cttggttgta
     2701 cattgcgccg tgcggtttga cataagccat ttccaaaccc tgatcacggc acaaggcctg
     2761 caatgcgccc aactggtaat tcagacacgc ccgcaaatcg gcttcggaca gattcatttc
     2821 ggtacggccg aagttttccc gatcgggata gccggggtgt gctccgatgc gcacgccgtt
     2881 ttgttgggca tacgccaatg ccgcccgaat atcggcaatg ctgccggcgt gttgggcgca
     2941 ggcgatgttg gccgaagtaa tcagctgcaa caaggcttcg tcgctgccgc agccttcggc
     3001 gagatcggcg tttaaatcaa cctgcttcat gggtgattct ccgtatttgg ttcagatagg
     3061 cttggttttt gcgccgcagg gcggtggctt ctttcaagcc gattattttg aatttgactt
     3121 tgctgccgaa gcgcacctgt gccagcctgc ccaaatcggc ggcggcaacg gtagcgattt
     3181 tcggataacc gccggtggtt tgcgcatcgg ccagcaggat aatcggtttg ccgccgggcg
     3241 gcacctgcac ggttcctgcc tgaacagcgt gggacagcat ttccaaaggt tgcgacaggg
     3301 tcagcggctg tccgtcgaag cggtagccca tgcggttgct atcgctttgc agcgtccacg
     3361 tttcccgttc cagattcaga cgcccttttt cactgaaagc ggcatattcc gacgaaggaa
     3421 caaggtggac ggtatcggta aacggtatcg gggcaatgcc gactttggac aattcctgcg
     3481 cacctttgcc gatggggaga taatcgcctt tttgcagcat tctgccctga tggccgccga
     3541 aaccggcttt caggtcggta cttctcgaac ccatcacttc cggcacatca aatccgcccg
     3601 ccacgcacac atagccgtac atgccctgca cggcacgcac cattttcaag gtctgccctt
     3661 tgcgggcggt ataacgccaa tacgaataga ccggttcgcc gtccaattcc gcctgataca
     3721 cggcaccggt gagacaaaac ggcgtatccc gttcaaacac cagcattatc ccgcccaaag
     3781 cgatttcgat tgcggccgtg ccttcgtcgt tgcccaataa aatattgccc gccgccaaag
     3841 caaccgtgtc catcgcaccg gcatgaccga tgccgtaacg ccggtgtccg tagcgtccgg
     3901 tatcctgaat atgcgccggt gcctgcactg ccgaaacgtg aatcatggct caatcctttc
     3961 tgcaacaaag cggacttggt cacccgccgc cagcagggtc ggcggattca aatcggctcg
     4021 gaacaagggt aattcggttc tgccgataat ctgccagccg ccgggcgaag cgaacggata
     4081 cacaccggtc tgactgccgc cgataccgac cgaaccggca ggaacggacg ttctcggcac
     4141 ggcacggcgg ggcgtgtgca atgcttcggg caagccgccc agataaggga aaccgggctg
     4201 gaagcccatc ataaatacgg tataagtttg cgccgtatgg cggcggacga tttcggaaat
     4261 aaccgtctga tggaaagcag cgacttccgc caaatccggg ccgtattcgc cgccgtagca
     4321 gacgggaatt tccaccagtt tgccctgatg gtctgtaacg gcggtgtgtt cccacacata
     4381 ttgcaattca tcggcaagcg tcgccaaatc ggtatcgaaa cgggtgaaca cggtcagatt
     4441 gttcatgccg accaccactt cctcaatcct gtcgtgctgc ccgagcgcag cggcaaacgc
     4501 ccacaacttt tgctgtttgc ccagttcgga aggtgcattc agtcggcaga ccaaagcgga
     4561 ttcgctgatt ggtgtgatct ctattctcat ttgttgttca ttttggttat gttttaatga
     4621 atctatatgc aggggcggcg gtttgtcaat atcttctgtg ctgcatcatc aaaccgtcga
     4681 ttggaaaagt gctgccctgc cgctgcactt tttcagacga ccttaaaccg tttctattaa
     4741 aatagcgcat tccacttttc agacggcatc tctatgtttc ccgaccaatc cgcccccaac
     4801 ctgctgcaag gcttgaatcc cgaacaactt tccgccgtaa cctggccgcc gcaatccgcc
     4861 cttgtgctgg caggcgcggg cagcggcaaa acgcgcgtgc tgaccacgcg tatcgcatgg
     4921 cttttgcaaa ccggtcaggc aagcgtgcac agcattatgg cggtaacgtt taccaacaaa
     4981 gccgccaaag aaatgcaaac ccgactcggc gcgatgattc ccgtcgacgt ccgcgctatg
     5041 tggctcggca cgttccacgg cctctgccac cgctttctac ggctgcacca ccgcgatgcc
     5101 ggtttgccgt cttcctttca aatcctcgac ggcggcgacc agctttccct catcaaacgc
     5161 ctgctcaaaa gcctcaacat cgccgaagaa atcatcgcgc cgcgttcgct gcaaggcttt
     5221 atcaacgcgc aaaaagaatc cggtttgcgc gcttccgtct tgagtgcgcc cgatccgcac
     5281 acaagccgca tgattgaatg ctacgccgaa tacgacaaaa tctgccaacg cgaaggcgtg
     5341 gtcgattttg ccgaactcat gctccgcagc tacgaaatgc tgcaaagcaa cgaaatcctg
     5401 cgccagcatt accaaaaccg cttcaaccac attctcgtcg acgagttcca agacaccaac
     5461 aaactgcaat acgcctggct caaactcatg gcgggcggca acgcggcagt gtttgccgtc
     5521 ggcgacgacg accaaagcat ctaccgcttc cgtggcgcaa acgtcggcaa tatgaccgcg
     5581 ctgatggaag agttccatat caacgcgccc gtcaaactcg aacaaaacta ccgctctgtc
     5641 ggcaacatcc ttgccgccgc caatgccgtt atcgaaaaca acgacgaacg actcggcaaa
     5701 aacctgcgca ccgacgccga agcaggcgac aaaattcgct actactccgc ctttaccgac
     5761 ctcgaagaag cccggttcat cgtggacgaa accaaagccc tcgaacgcga aggctgggat
     5821 ttggacgaaa tcgccgtcct ctatcgcagc aacgcccaat cccgcgttat cgaacaaagc
     5881 ctgttccgca gcggcatccc ttacaaaatc tacggcggcc tgcgctttta tgaacgccaa
     5941 gaaatcaaac acgcgctcgc ctaccttcgc ctcgccgtta atcccgacga cgacaacgcc
     6001 ctcttgcgcg tcatcaactt cccgccgcgc ggcatcggcg cacgcaccat cgaaaacctt
     6061 cagacggcct caaacgaaca aggcatcacc ctttggcagg cagcctgcaa cgccggcgca
     6121 aaagccacca aagtcgccgc cttcgtccgc ctcatcgaag ccctgcgcaa ccaagtcgga
     6181 caaatgcccc tgcccgaaat catcgtcggc atcctcaaag acagcggcct gaccgaacac
     6241 tacaaaaccc aaaaaggtga caaccaagac cgcctcgaca acctcgacga actcgtcaac
     6301 gccgccatcg agttcaaacc cgaagacagc aactttgaaa tcctgcctga aaacatttca
     6361 gacgaccccg cctttcccat cctcgccttc ctaagcaacg ccgccctcga atccggcgaa
     6421 aaccaagcag gcgcggacga aaaagccgtc caactcatga ccgtccacgc cgccaaaggc
     6481 ttggaattta acgccgtctt cctcaccggc atggaagaag gccgcttccc cagcgaaatg
     6541 agccttgccg aacgcggcgg cctcgaagaa gaacgccgcc tcatgtacgt cgccatcacc
     6601 cgcgcccgca aacgcctcta catcaccatg gcacagcaac gcatgctgca cggacaaacc
     6661 caattcggca tcgtctcccg cttcgtcgaa gaaatcccac ccgaagtatt gcactacctg
     6721 tccgtcaaaa agaccgccta cgacagctac ggcagcccgc gccaaaccgc cgcatccaaa
     6781 gacaaaatca tcgacgacta caaacagccc caaacctacg caggtttccg tatcggacaa
     6841 aacgtccgcc acgccaaatt cggcaccggc gtgattatcg atgccgcaga taaaggcgaa
     6901 tccgcccgac tgaccatcaa cttcggcaaa cagggcgtga aagagttgga taccaagttt
     6961 gcgaaattgg aagcgatgta gaagggagat ggcagaggcc gtctgaaaac gggaacaaca
     7021 atttttgccc aaacctccgc cgtcattccc gcgcaggcgg gaatccagac ctttcggcac
     7081 gggaacttat cggataaaag gtttctttag attccacgtt ctagattccc gcctgcgcgg
     7141 gaatgacgga cggtaaaagc cgggtataga tacctaaccc ttgtttatag ctgaaaatca
     7201 ataaggaaga acattatgtc ccaagttttt aaagattttg atttgtcctc cgtatgggaa
     7261 tccgacagtt gggcagatga aaactacaaa gaagccccgt ttacccctga aattttggct
     7321 gccgtagaaa gtgaactggg ctataaattg ccgcaaagtt ttattgaatt gatggcggta
     7381 caaaacggcg gaatatttgt caaaaactgt tttccgacca cacagagaaa ttcgtgggcg
     7441 aaagatcatg tgcaaatttg cgaggtatcg ggaatcggtt ttgaaaaaga aggaagcctg
     7501 tgcggcgaaa cagggcaaaa actttggctg gaagaatggg agtatccgcc catcggtgtg
     7561 tattttgcca acgacccgtc aggcggtcat gccctatttg ccttagacta tcgggagtgt
     7621 ggaagagacg gagagccgaa agtggtgttt gtcgaacaag aatcggattt ttaaatcgtc
     7681 gaacttgccc ccgattttga aacctttatt cacagcttgc ggcatgaaga tgagtttatt
     7741 gacgaggaaa tataaaacgg cggttgaaaa accagaatca tcaaaggaaa acgggcggaa
     7801 taacagataa ccgattgaat ccgtaaggaa aacggtttgt aggaacgtgc catccaagac
     7861 ctttgcaaag aaaaactgtc cccgacagca ttgacattat taacagaact tatcaatttt
     7921 ggagctatct caaatataat tcggttatcc tgttgtatcc attaaatcat atgcttcaat
     7981 taaatgttgt tctagctctt gcactgattg aggattgcga attcctgata caatctcaaa
     8041 ttcttctgca tctatgcaaa cacctgcata aatttcaata acaagggaac gcaataattg
     8101 aagctcttct cttgttaaag aaataataat gtcatcacct gtgtaattga ttatattcat
     8161 aataatttta tttttgtttg tcaaagtaag ttttgcctaa ggttggtcta aatgcagttc
     8221 caccatcttt tgaatttggg tctctgatta caattgctcc agacttatca tcccaaattg
     8281 ctcttatgtg tttggattgt aatcttcgaa ttcccaagaa aaaaatcgta ataagtttga
     8341 aagtgtcaaa tcccaagttt cttttgagca atattctaat attttatcaa tttcactttt
     8401 aataatctta tgatcaaact gttctaatat taatgcatta gaccaaaaaa aaccttcttt
     8461 attacaatga tgggaaatcc atttaggaga acaaatgcaa agtgaaaaaa tagatgagcc
     8521 ttgttctcct tcgattccga tatccaaatc tatccatcta tggaaattat ctggaatttc
     8581 gggggtaaat ttttcaaaat caatatcata taaatttatg ctttttaaat ccaatttaat
     8641 cattagggct gtcctagata aatagggaaa ttcaaattaa gttagaatta tccctatgag
     8701 aaaaagtcgt ctaagccggt ataaacaaaa taaactcatt gagctatttg tcgcaggtgt
     8761 aactgcaaga acagcaacag agcctgacag cattgtttat acggattgtt atcgtagcta
     8821 tgatgtatta gatgtgagcg aatttagcca tttccgtatc aatcacagca cacattttgc
     8881 tgaacgacaa aaccatatca atggaattga gaatttttgg aaccgggcaa aacgtcattt
     8941 acgcaagttt aacggcattc ccaaagcgca ttttgagctg tatttaaagg agtgcgaatg
     9001 gcgttttaac aacagtgaga taaaagttct tgttccattt taaaacaatt agtaaaatcg
     9061 agtttatctt agttgtccag gacagcccca ttatttttat aacaccgtga agccgcacag
     9121 cagtttgaac agtgatacgc cgtttgcggg cttacgagtt tattttcccg gcctgcagtt
     9181 tgagcaatac ggtgatttcc tacggttaat acaaatgttt acacattgat acatttcatt
     9241 tatagttccg cctatttgaa aatagaaaat atgaattcga ccgcaagtaa aaccctgaaa
     9301 ggattgtcgc tggtgttttt cgcctctgga ttctgcgccc tgatttacta ggtcagctgg
     9361 cagaggcttc tattcagtca cataggtatc gatttaagtt cgattactgt cattatttct
     9421 gtatttatgg tcggcttggg tgtaggtgcg tatttcggtg gacgcattgc tgaccgtttt
     9481 ccttcaagta tcatccccct gttttgcatc gctgaagtat ccatcggtct gttcggtttg
     9541 gtaagcaggg gtctgatttc cggcttgggg catcttttag ttgaggctga tttgcccatc
     9601 atcgctgctg ccaatttcct cttattgctg cttcctacct ttatgatggg cgcgaccttg
     9661 cccttgctga cctgtttttt taaccggaaa atacataatg ttggcgagtc tatcggtacc
     9721 ttatattttt tcaacacttt gggtgcggca ctcggatcgc ttgccgccgc cgaatttttc
     9781 tacgtctttt ttaccctctc ccaaaccatt gcgctgacag cctgctttaa ccttctgatt
     9841 gctgcttcag tatggctgcg ttacagaaag gatggatata gtgaacacta aaccgaatac
     9901 tagtttgatt tatatgcttt ctttccttag cggcttattg agcttgggta tagaagtctt
     9961 gtgggtaagg atgttttcgt tcgcagcaca gtccgtgcct caggcatttt catttactct
    10021 tgcctgtttt ctgaccggta tcgccatcgg cgcgtatttt ggcaaacgga tttgccgcag
    10081 ccgctttgtt gatattccct ttatcgggca gtgcttcttg tgggcgggta ttgccgactt
    10141 tttgattttg ggtgctgcgt ggttgttgac gggtttttcc ggcttcgtcc accacgccgg
    10201 tatcttcatt accctgtctg ccgtcgtcag agggttgatt ttcccgctcg tacaccatgt
    10261 gggtacggat ggcaacaaat ccggacgaca ggtttccaat gtttatttcg ccaacgttgc
    10321 cggcagtgca ttgggtccgg tccttatcgg ctttgtgata cttgatttct tgtccaccca
    10381 acagatttac ctgctcatct gtttgatttc tgctgctgtc cctttgtttt gtacactgtt
    10441 ccaaaaaagt ctccgactga atgcagtgtc ggtagcagtt tccctaatgt tcggcatcct
    10501 catgttccta ctgccggatt ctgtctttca aaatattgct gaccgtccgg ataggctgat
    10561 tgaaaacaaa cacggcattg ttgcggttta ccatagagat ggtgataagg ttgtttatgg
    10621 ggcgaatgta tacgacggcg catacaatac cgatgtattc aatagtgtca acggcatcga
    10681 acgtgcctat ctgctaccct ccctgaagtt tggcatacgc cgcattttcg tcgttggatt
    10741 gagtacaggt tcgtgggcgc gcgtcttgtc tgccattccg gaaatgcagt cgatgattgt
    10801 tgcggaaatc aatccggcat accgtagcct tatcgcggac gagccgcaaa tcgccccgct
    10861 tttgcaggac aaacgtgttg aaattgtatt ggatgacggt aggaaatggc tgcgtcgcca
    10921 tcctgatgaa aaattcgacc tgattttgat gaatacgact tggtactggc gtgcctattc
    10981 caccaacctg ttgagtgcgg aatttttaaa acaggtgcaa agccacctta ccccggatgg
    11041 tattgtaatg tttaatacca cgcacagccc gcatgctttt gctaccgccg tacacagtat
    11101 tccctatgca taccgctatg ggcatatggt agtcggctcg gcaaccccgg tagttttccc
    11161 taataaagaa ctgctcaagc aacgtctctc ccggttgatt tggccggaaa gcggcaggca
    11221 cgtatttgac agcagcaccg tggatgctgc agcacaaaag gttgtctctc gtatgctgat
    11281 tcagatgacg gaaccttcgg ctggggcgga agtcattacc gacgataata tgattgtaga
    11341 atacaaatac ggcagaggga tttaaccgtc ttaaagggtt tcaggcaacg caggttttag
    11401 gtaacgtcct gctagttcaa aaaaaccgca tcacagcagt cgggacaaaa tggtttaaac
    11461 attttgtccc gaattcttat tcctatatat agtggattaa caaaaatcag gacaaggcga
    11521 cgaagccgca gacagtacaa atagtacgga accgattcac ttggtgcttc agcaccttag
    11581 agaatcgttc tctttgagct aaggcgaggc aacgccgtac tggtttttgt taatccacta
    11641 taccacgaat tacggtgtaa aaatttatat gaccttataa aatcaaataa gaatcgttat
    11701 cataacatga ttgtatttat tgggtttttt tgggcgtttt gccgatattt accttttaat
    11761 ggtttttgaa attcgctaaa atacgaaatt attgtagaaa ttttgttaac ggatttgggt
    11821 gtaaccatgt tgtccgctta ctttcccgtc tttgtcttta tcctcgtcgg cctcgcggcc
    11881 ggcgtgctgt ttatcctgct cggcacgatt ttgggcccga aacgccacta cgccgaaaaa
    11941 gacgcgcctt acgaatgcgg ttttgaggcc tttgaaaacg ccaggatgaa gttcgacgtg
    12001 cgctattacc tcgtcgccat cctctttatc ctgtttgatt tggaggtcgc gttcatgttg
    12061 ccgtgggcag ttgtgttcaa agatttgggt gcgtacggct tctggtctat gctggtgttt
    12121 atcgtcgttc tgacggtagg ctttgtttac gaatggaaaa aaggtgcgct ggaatgggaa
    12181 tagaaggcgt tttgaaaaaa ggtttcatca ccaccagcgc ggatacggtg ttgaactata
    12241 tgcgtaccgg ttcattgtgg ccggttactt tcggcttggc ctgctgcgcc gtggaaatga
    12301 tgcatgcggg tatggcgcgt tatgaccttg accgcttcgg catcattttc cgtccgtccc
    12361 cacgtcaggc cgacctgatg attgtggcag gtacgctgac caacaaaatg gcgcccgccc
    12421 tgcgccgcgt atacgaccag ctcgccgagc cgcgctgggt attgtctatg ggctcgtgtg
    12481 ccaacggcgg cggctattat cactattctt attccgttgt gcgcggtgcc gaccgcgtcg
    12541 tgccggtaga cgtttatgtg ccgggttgtc cgccgactgc ggaagccctg atttacggtc
    12601 tgattcagct ccaacaaaaa atcaagcgca cttccaccat cgcgcgtgac gagtaaggag
    12661 aggacgatat ggcaagcatt caaaacttat acgaaaccgt cagccgcgtt ttgggcaatc
    12721 aggcaggcaa agtcatttcc gctttgggcg agattaccgt cgagtgtctg cccgaacact
    12781 atatttcagt catgaccgca ttgcgcgacc atgaagagtt gcatttcgag cttctggtcg
    12841 acttgtgcgg tgtcgattac agcacttaca aaaacgaagt atggcagggc aaacgctttg
    12901 ccgtcgtcag tcagttgctt tccgttaaaa acaatcaacg catccgcgta cgcgtctggg
    12961 tttcagacga cgacttccct gtagtcgaat ccgtagtcga tatttacaac agcgcggatt
    13021 ggtacgaacg cgaagccttc gatatgtacg gcatcatgtt caacaaccac ccggacttgc
    13081 gccgcatcct gaccgattac ggctttgtcg gacatccgtt ccgcaaagac ttcccgattt
    13141 ccggctatgt ggaaatgcgt tacgacgaag agcaaaaacg cgtgatttac caacctgtta
    13201 ccattgagcc gcgcgagatc acgccgcgta tcgtccgtga ggagaactac ggtggccaat
    13261 aaattaagaa actacaccat caacttcggc ccgcaacacc ctgcggcgca cggcgtattg
    13321 cgtatgattt tggagctgga cggcgaacaa atcgtccgtg ccgacccgca tatcggcctc
    13381 ctgcaccgag gcacagagaa actggcggaa accaaaacct acctgcaagc cctgccttat
    13441 atggacaggc tggactacgt ttccatgatg gtcaacgagc aggcgtattg cttggcggta
    13501 gaaaaacttg ccggtatcga tgtgcctatc cgcgcccaat acatccgcgt gatgtttgcc
    13561 gaagtaacgc gcatcctcaa tcacttgatg ggcatcggtt cgcatgcctt cgacatcggc
    13621 gcgatgaccg ccattcttta cgccttccgc gaccgtgaag agctgatgga cttgtacgaa
    13681 gccgtttccg gcgcgcgtat gcacgccgcc tacttccgtc ccggcggcgt ttaccgcgac
    13741 ctgcccgact tcatgcctaa atacgagggc agcaaattcc gcaatgccaa agtattgaag
    13801 cagctcaacg aatcccgcga aggcacgatg ctcgacttta tcgatgtctt ctgcgaacgt
    13861 ttcccgaaaa atatcgacac actcgaaacc ctcctgaccg acaaccgtat ttggaaacag
    13921 cgtaccgtcg gcatcggcgt cgtctccccc gaacgtgcca tgcaaaaagg ctttaccggc
    13981 gtgatgttgc gcggttcggg tgtggaatgg gacgtgcgta agaaacagcc ttacgaagtg
    14041 tacgacaaaa tggatttcga catccctgtc ggcgtgaacg gcgactgcta cgaccgctac
    14101 ctctgccgta tggaagaaat gcgccaatcc gtacgcatca tcaaacaatg ctccgagtgg
    14161 ttgcgtgtca atccgggtcc ggtcattacc acaaaccaca aattcgctcc gcccaaacgt
    14221 accgaaatga aaacaggtat ggaagacctg attcaccatt tcaaactctt taccgagggt
    14281 atgcacgttc ccgagggcga gacttacacc gctgtcgaac atccgaaagg cgagttcggc
    14341 gtttacatca tttcagacgg cgcgaacaaa ccctaccgcc tgaaaatccg cgcacccggc
    14401 ttcgcccatc tgcaaggtat ggacgaaatg gcaaaaggcc atatgctcgc cgacgtcgtt
    14461 gccatcatcg gtacgcagga catcgtattc ggggaggttg accgataatg ttatccgcag
    14521 aatccttaaa acaaatcgac atcgagttgg cgaaatatcc cgccgaccaa cgccgctctg
    14581 cgattatggg cgcattgcgt attgcccaga ccgaaaaagg ctggctcgcc cccgaaacca
    14641 tcgcttttat cgccgactac atcggcatct cgcctgcaca agcctacgaa gtcgctacct
    14701 tctacaatat gtacgacctt gagcctgtcg gcaaatacaa actgaccgtt tgtaccaacc
    14761 tgccctgcgc cctacgcggc ggtatggcga ccggcgaata cctcaaacaa agactcggta
    14821 tcggctacgg cgaaaccacg cccgacggca aatttaccct tgtcgaaggc gaatgcatgg
    14881 gcgcgtgcgg cgacgctccg gttatgctgg tcaacaacca cagcatgtgc agctttatga
    14941 ccgaagaggc gattgagaag aaactggcgg agttggagta ggtcgtctga aacgacgatt
    15001 taaacgtagg tcggatactt gtatccgaca gagtgggtaa aaaggcaaaa tgtcggattt
    15061 aagaatccgc cctactgaaa taccgaaatg ccgtcattcc cgcgcaggcg ggaatccacc
    15121 ggtaagattc ggtttctgaa tttaataaga cattgcttac cattgaggat ggattcccgc
    15181 ctgcgcggga atgacgacag acaagcaagt ggtcgagatc caacaaaaac gattaaaggt
    15241 cgtctgaaaa tatcgatttg ataaactaga ttttatttca gacgacgtta caagccggta
    15301 cacaaagaca tcttaaggtc gtctgaaaca gcggccgcaa ccgatacgaa aacaaacagg
    15361 cacaccaaaa atggctattt accaatcagg cgtgattttt gaccaagtgg ataccgccaa
    15421 tcccgactgc tggacattgg acgaatacgt caaacgcggc ggctataccg ccctgcgtaa
    15481 aattctgtcc gaaaacatct cgcaaaccga tgtgattgac gaagtcaaaa cctccggctt
    15541 gcgcgggcgc ggcggtgcgg gcttcccgac tggtttgaaa tggagcttta tgccccgttc
    15601 cttcccgggc gaaaaatatg tggtttgtaa caccgacgaa ggcgaaccgg gtacatttaa
    15661 agaccgtgac atcatcatgt tcaatcctca tgccctgatt gaaggcatga ttatcgccgg
    15721 ttacgcgatg ggtgcgaaag ccggctacaa ctatatccac ggcgaaatct tcgaaggcta
    15781 ccaacgcttt gaagccgctt tggagcaggc gcgtgccgca ggctttttgg gtaaaaatat
    15841 tttgggttcg gattttgaat ttgaactctt cgcccaccac ggctacggcg catatatttg
    15901 cggcgaagaa accgcattgc tcgaatcgtt ggaaggcaaa aaaggccagc cgcgcttcaa
    15961 accgccattc cctgcttcgt tcggcctgta cggcaaaccg accaccatta ataatactga
    16021 aaccttttcc tccgtcccat tcattgtccg tgatggcgga caggcatttg ccgataaagg
    16081 tattccgaat gcaggcggta ccaaattgtt ctgtatttcc ggccatgtcg agcgtccggg
    16141 caactatgaa gtgccattgg gcacaccgtt tgccgaagtc ttgaaaatgg caggcggtat
    16201 gcgcggcggc aaaaaactca aggccgttat tcccggcggt tcgtccgcgc ccgtgttgcc
    16261 tgccgatatc atgatgcaga ccaatatgga ctatgactcg atttctaaag caggctcgat
    16321 gttgggttcc ggcgcgatta tcgtcatgga cgaagatgtg tgcatggtca aagcccttga
    16381 acgcctgagc tacttctact atgacgagtc ttgcggccaa tgtacgcctt gccgagaagg
    16441 tacgggctgg ctctaccgca tcgtccaccg catcgtagaa ggcaaaggca aaatggaaga
    16501 tttggatttg ctggattccg tcggcaacca aatggcaggc cgcaccatct gcgcccttgc
    16561 cgatgccgcc gttttccctg ttcgcagctt taccaagcat ttccgtgatg agtttgtgca
    16621 ttacatcgaa cacggcggac cgatgaaaga gcataagtgg ggagggtggt aatggtggaa
    16681 gctaaaattt ttattctata cggtgcagcc aacaaaggta agagtacgac actcaatacg
    16741 ctttttaatc agatttgttg gaaattttct aaatttctag tcttttttga aagacatgga
    16801 aacggcttag attttgttgc agtatttgat catgaaggtc agaggattgg tttttattca
    16861 tctggtgata atgaatacga ggttaggaga aatttataca aactttattc gcataattgt
    16921 gattttattt ttggcacgtc aaggacacgg ggtggtagtt gcgatgcagt aggatgttat
    16981 gcagagttat tgcatggcga tgtaaatata attaattggt gtgaaaagtt tgagcctaca
    17041 gatgaagaca atgagcgtgc tgttaaagag ttatttaagt catttaaaaa tataataaat
    17101 gagttatagt tttagttggt tttatattgg ttaaaagcaa aatgctaaaa atttaacttt
    17161 gccgtcattc ccgcgtaggc gggaatccat agtggaattt acagaacccg atatttgaaa
    17221 agcagttacc gaaaacccaa gaatggattc ccgcctgtgc gggaatgacg gcaaggtggc
    17281 agtaaacgtt ttaaacagta ttgattgtca atgaaactca aaaggccgtc tgaaacccat
    17341 ttttcagacg atctccataa agaattattt atcaaatacc cgtaactagg aacgaaccat
    17401 gttacaaatc gaaatcgacg gcaaacaagt atctgtggag cagggcgcga cggtgattga
    17461 agccgcgcac aagctcggta cttatatccc gcatttctgt taccacaaaa aactttccat
    17521 cgccgccaac tgccgtatgt gtttggtgga cgtggaaaaa gcccccaaac ctctgccagc
    17581 ctgtgccacg ccggtaacgg acggcatgat tgtgcgtacg cattcggcaa aagcccgaga
    17641 ggcgcaagaa ggcgtgatgg agttcctgct catcaaccat ccgcttgatt gtccgacctg
    17701 cgaccagggc ggcgaatgcc agttgcagga tttggcgatg ggctacggca aaaccaccag
    17761 ccgttatacc gaagaaaaac gttccgtcgt cggcaaagat atgggacctt tggtttccgc
    17821 cgaggaaatg agccgctgca tccattgcac ccgctgcgtg cgattcaccg aagaaattgc
    17881 cggtttgcag gaaatcgcga tggcgaatcg cggcgaacac tccgaaatca tgccttttat
    17941 cggcaaagca gtggaaaccg aattgtcagg caacgtcatc gatttgtgtc ctgtcggtgc
    18001 attgaccagc aaaccgttcc gcttcaacgc gcgtacttgg gagctgaacc gccgcaaatc
    18061 cgtttctgcc catgatgctt tgggcagcaa tttgattgtg caaaccaaag accataccgt
    18121 ccgccgcgtg ctgccgttgg aaaacgaagc gattaacgaa tgctggctgt ccgaccgcga
    18181 ccgtttcgcc tacgaaggcc tgtatcacga aagccgtctg aaaaacccga aaatcaaaca
    18241 gggcggcgag tggatggacg tggattggaa aaccgcgttg gaatatgtcc gcagcgcgat
    18301 tgaatgtatc gccaaagacg gtaaccaaaa ccaagtcggc atttgggcga acccgatgaa
    18361 tacggttgaa gagctgtatc tggcgaaaaa actcgccgac ggcttgggtg ttaaaaactt
    18421 tgccacccgt ttgcgccaac aagacaaacg tctttcagac ggcattaaag gtgcgcaatg
    18481 gttgggacaa agcattgaat ctttggctga caacgatgcc gtattggtag tcggtgcgaa
    18541 cttgcgcaaa gaacagccgc tcctgaccgc ccgcctgcgc cgcgccgcca aagaccgtat
    18601 ggctttgagc gtattggccg gcagtaaaga agaattgttt atgccgcttc tgtctcaaga
    18661 agccgcacat cccgacgagt gggcagaccg tctgaaaaaa ctgtctgcca atgcggaaca
    18721 cgccgttacc gccagcctga aaaacgctga aaaagcagcg gtgattttgg gcgcggaagt
    18781 gcaaaaccat cctgattacg ccgccattta tgccgccgcg caagagctgg cggacgcgac
    18841 cggcgcagtg ctgggtattt tgccgcaagc cgccaacagt gttggtgcgg atgtcttgaa
    18901 tgtaaactcc ggcgagagcg ttgccgaaat ggcaaacgcg ccgaaacagg cagtcttgtt
    18961 gctcaacgtc gagccggaaa ttgatacggt tgacggtgca aaagccgtag ccgcgttgaa
    19021 acaggcaaaa agcgtgatgg cgtttacgcc gtttgtcagc gaaacgctgc tggacgtgtg
    19081 cgacgtgttg ttgccgattg cgccgtttac cgaaacctca ggcagcttca tcaatatgga
    19141 aggccgtctg caatctttcc acggcgtggt acaaggcttc ggcgattcgc gtccgctgtg
    19201 gaaagtgttg cgcgtattgg gcaacctgtt tgacctgaaa ggttttgaat accacgatac
    19261 cgctgcgatc ttgaaagacg cgctggatgc agaaagcctg ccgtccaaac tggacaaccg
    19321 cagtacatgg acaggggagg gcgttcagac ggcatcagac cgcctcgtgc gcgtgggcgg
    19381 agtgggcatc tatcacaccg atgccatcgt gcgccgttcc gcaccgttgc aggcaaccgt
    19441 ccatgccgcc gtgcctgccg cgcgtgtgaa tccgaacact ttggcgcgct tgggcctgca
    19501 agacggacaa accgctgttg ccaaacaaaa cggcgcaagc gtatcggttg ccgtcaaagc
    19561 cgatgccggt ttgcctgaaa acgtggtgca tctgtcgctg cataccgaaa atgccgcgct
    19621 gggtgcgttg atggacacta ttgaactggc gggagcttga tcatgcaaga atggttccaa
    19681 aacctctttg ccgcaacgct cggtctgggc gatttgggta tcaccgtggg cttggtggta
    19741 tccgtcatcg tcaaaatcgt gattatcctg attccgctga ttctgaccgt cgcctacctg
    19801 acttatttcg aacgtaaagt catcggcttc atgcagcttc gcgtcggccc gaacgtaacc
    19861 ggcccgtggg gcctgatcca gccgtttgcc gatgtgttca aactcttgtt taaagaggta
    19921 acccgtccga agctgtcaaa caaagccctg ttctatatcg gcccgattat gtcgcttgcc
    19981 ccgtctttcg cggcgtgggc agtgattccg ttcaacgaag aatgggtgct gaccaatatc
    20041 aatatcggcc ttttgtacat cctgatgatt acctcgctgt cggtttacgg cgtgatcatc
    20101 gcgggctggg cttccaactc aaaatattcg ttcttgggcg caatgcgtgc ttccgcgcaa
    20161 agcatctcct acgaaatcgc catgagcgcc gcgctggtgt gcgtcgtgat ggtgtcgggc
    20221 agcatgaact tctccgacat cgttgccgca caagccaaag gcatcgcggg cggttccgtg
    20281 ttctcgtgga actggctgcc gctcttcccc atcttcatcg tctatctgat ttccgccgtt
    20341 gccgaaacca accgcgcgcc gtttgacgtg gcagagggtg agtctgaaat cgttgccggc
    20401 caccacgtcg aatactccgg cttcgcattc gcgctgttct tccttgccga atacattttc
    20461 atgattctga tttccgcgct gacatcgttg atgttcctcg gtggttggtt gtctccgttc
    20521 ccacaaagct ggggctttat cggcacgcct tccgcattct ggatgttcgt gaaaatggcg
    20581 gcggtgttgt actggtatct gtggatccgc gcaactttcc cacgctaccg ttacgaccaa
    20641 atcatgcgtt tgggctggaa agtgctgatt ccgatcggct tcgcctacat cgtgattttg
    20701 ggtctgtgga tgatttcacc gctgaatttg tggaaataag tttcagacga cgtatcaagg
    20761 tcgtctgaaa acgcagtttt gaatatttaa cagaatccct gttttgaggg aacataatat
    20821 ggctaactta gtaaaaacct ttctgcttgg cgaattggta aaaggtatgg gcgtaacgct
    20881 caaaaacttt tttgcccgca aagacacgat ttattttccc gaagagaaaa cgccgcaatc
    20941 cgtgcgtttc cgcggtctgc acgcgcaacg ccgttatcca aacggcgagg aacgctgcat
    21001 cgcgtgtaaa ttgtgtgaag ccgtttgccc cgcaatggcg atcaacatcg aatcggaaga
    21061 acgcgaagac ggcacgcgcc gcaccaagcg ttacgacatc gacctgacca agtgcatctt
    21121 ctgcggtttc tgcgaagaag cctgcccgac cgatgcgatt gtggaaaccc atatttttga
    21181 ataccacggc gagaaaaaag gcgacttgca catgaccaag ccgattcttt tggccattgg
    21241 cgacaaatac gaagctgaaa tcgccaaacg caaagccgct gacgcgccgt atcgttaatg
    21301 ctttggggct tcttggaagg ttttaaatat ggaaggactg attaatgcat tgaaatattt
    21361 agccgaacat gagccaatag ataattttga agaaattaga actagaaata gtccgattga
    21421 gttgccaagt ggattaagta attttgaaca aaatattttt ttaaaagaaa atttatcccc
    21481 aaaattacaa aatgatgata gcttgaagac gcattattgg attatccgtg aatggggtgg
    21541 gattaaaagt tttaaacaat ctgctgaaaa tagccagctt attcgtcaat ttttatcgga
    21601 acttaattcg ggaaaattga gtagtggttt gttgaaaatt tcatcattat ctaaattggc
    21661 ttcttttata gattgtgagc gattcgccat ttatgattca cgcgctattt tttcgttgaa
    21721 ttggttgttg tttaaattta caaatgcaga tttgtttttt cagccacaag gtagaaatag
    21781 ggaactagaa atccgaaata tgaacgtatt gtttcatttt tctgatatca aaccgaatta
    21841 tcggaaacca gacgtttcgt ttcatcaata ttgtggattg ttacaagatt tggcgaaaca
    21901 agtttatggt aaacaagcaa aaccgtatca catagaaatg ttgttattca aaattgcgac
    21961 aacgtggatt tgtgcggata tggatcaact gattaagttt gattgtttgc gtaaccagga
    22021 ttttcagact gcttgaaacc atatttttga ttaataaaga aagcatagac tatgactttc
    22081 caactgattt tattttatat ttttgcagtg ataattcttt atggcgcgat caaaaccgtc
    22141 accgctaaaa atcctgttca cgccgctttg catctggtgt tgaccttctg cgtgagcgcg
    22201 atgctttgga tgctgatgca ggctgagttt ttgggcgtga cgctggtggt ggtttacgtc
    22261 ggcgccgtga tggtgttgtt cctgttcgtc gtgatgatgt tgaacatcga cattgaagaa
    22321 atgcgtgccg gtttctggcg gcacgcgcct gttgccggtg tggtcggcac attgttggca
    22381 gttgccctga ttctgattct ggtcaatccg aaaaccgacc ttgccgcatt tgggctgatg
    22441 aaagacattc ccgccgatta caacaatatc cgcgatttgg gcagccgtat ttataccgat
    22501 tatctgctgc cgtttgaatt ggcggcggta ttgctgctgt tgggtatggt ggccgcgatt
    22561 gcgctggttc accgtaaaac ggttaatccg aaacgtatgg atcctgccga ccaagttaaa
    22621 gtacgcgccg accaaggccg tatgcgcttg gtgaaaatgg aagccgtgaa accgcaaacc
    22681 gaatctgccg aagaaagcga agtttcagac gacctcaagc cgaaagagga gggcaaagca
    22741 tgattacctt gacgcattat ctggtattgg gtgcgctcct gttcggtatc agcgcgatgg
    22801 gtatctttat gaaccgcaaa aacgtgctgg tcttgctgat gtccatcgag ctgatgcttt
    22861 tggcagtgaa cttcaacttt atcgcctttt cgcaacattt gggcgatact gccggacaaa
    22921 ttttcgtatt tttcgtactg accgttgctg ctgccgaatc tgccatcggt ttggcgatta
    22981 tggtgctggt gtaccgcaac cgacaaacga ttaacgttgc cgatttggac gagttgaaag
    23041 ggtaaaggta ggttgggtcg agacctgaca agacaccgat gccgtctgaa aacccgatag
    23101 gaaaaacgat gaaatccata gacgaacaaa gcctgcataa tgcccgccgc ctgtttgaaa
    23161 gcggcgacat cgaccgtatc gaagtcggta ccaccgcggg cctgcaacag attcaccgtt
    23221 acctgttcgg cggcttatat gattttgcgg gtcaaatcag ggaagacaac atttccaaag
    23281 gcggttttcg ttttgccaac gccatgtatt taaaagaggc tttggttaaa atcgagcaga
    23341 tgcccgagcg gacttttgaa gaaatcatcg ccaaatatgt tgaaatgaac attgcccatc
    23401 cgtttttgga gggtaatggc agaagtaccc gcatctggct ggatttggtg ctgaagaaaa
    23461 acctgaaaaa agtcgtgaac tggcaaaatg taagcaaaac cctgtatttg caggcgatgg
    23521 aacgcagccc cgtcaacgat ttagaactgc gctttttgtt aaaggacaac ctgaccgacg
    23581 atgtggacaa ccgtgaaatc atctttaaag gtatcgagca gtcgtattat tacgaagggt
    23641 atgaaaaagg ctgaaggtcg tctgaaaagc gatttcagac tgtttcagac gacctgattt
    23701 ggtaggtgat cagacgggag cggatgagaa aagaaattct aggtaagaat aatccggtct
    23761 gaaatattgg aagaagaatg atggataaaa atcagttaga acaagaattt cataaagcca
    23821 tgttaaatat ttatcaggag gctttgaatt tgccgcaacc ttacaaggcg acacgatttt
    23881 tacaaattgt aaatgaattt ggtggtaaag aggcggcgga taaattatta agtacggggg
    23941 aaaagaagac tcagaccggt tttacagagc tgattttgag tggtggcgga gtccacgcct
    24001 tgaaatacag tatggaatat ctggtgttac aaaagccgtg gtgtgattta tttactgaag
    24061 agcaattagc tgtggcacgc aaacgattgg agcgtgttgg atttgtttct ccgaagtaat
    24121 tttgtacgaa acaaacatag atttttaaat caatcggatt caatcaaatg aacgacatga
    24181 ctttatatct catcatcgcc ctcgtgcctt tggcaggctc gctgattgcg ggtttattcg
    24241 gcaacaaaat cggacgtgcc ggcgcgcata cggttacgat actcggtgtg gcggtgtccg
    24301 ccgtgctgtc ggcttatgtg ctgtggggat tcctcaatgg cagccgtgcc aagtttgacg
    24361 agaacgtcta tacctggctg acaatgggcg gtttggattt ctccgtcggc ttcttggtcg
    24421 atacgatgac ggcgatgatg atggtcgtgg taacgggcgt gtcgttgatg gtgcatatct
    24481 acaccatcgg ctatatgcac gatgaaaaag tcggctacca acgcttcttc agctatattt
    24541 ctttgtttac tttcagtatg ttgatgctga ttatgagcaa caacttcatc cagctcttct
    24601 tcggctggga ggctgtgggc ttggtatcgt atctcttgat cggtttctat ttcaaacgcc
    24661 cgagcgcaac atttgccaac ctgaaagcct ttttgatcaa ccgtgtcggt gacttcggct
    24721 ttttgctcgg tatcggcttg gtgcttgcct atttcggcgg cagcctgcgc tatcaagatg
    24781 tattcgctta tctgcccaac gtccaaaatg ccaccatcca gcttttcccc ggtgtggaat
    24841 ggtctttgat taccgtaact tgtttacttc tgtttgtcgg tgcgatgggt aaatcggcac
    24901 aattcccgct gcatgtctgg ctgcccgatt cgatggaagg cccgaccccg atttccgcat
    24961 tgattcacgc cgcaaccatg gttaccgccg gtctgttcat ggtatcgcgt atgtcgccga
    25021 tttatgagat gagtagcact gcgctgtctg tcatcatggt gattggtgcg attaccgccc
    25081 tgtttatggg tttcttgggc gtgattcaaa acgacatcaa acgtgtggtt gcgtattcca
    25141 ccctgtcgca actgggctat atgaccgtgg ctctgggcgc gtctgcctat tccgtggcga
    25201 tgttccatgt gatgacccac gctttcttta aagccttgct gttcttggcg gcgggcagcg
    25261 cgattatcgg tatgcaccac gaccaagata tgcgccacat gggtaacctg aaaaaataca
    25321 tgccgattac ttggctgact atgctgatcg gcaatttgtc gctgatcggt acgccgttct
    25381 tctccggctt ctactccaaa gattcgatta tcgaagcggc gaaatacagc accctgccgg
    25441 gcagcggctt tgcctatttc gccgtcctcg ccagcgtgtt tgttactgcg ttctacgcat
    25501 tccgccaata ctttatggtg ttccacggcg aagagaaatg gcgcagcttg cctgaacatc
    25561 atgacgatca tcatggcgaa gaacatcaca gcttgggcaa aaacgacaat ccgcacgaaa
    25621 gcccgttggt tgttaccctg cctttgattt tgcttgccat tccgtccgtc atcatcggct
    25681 acgttgccat cgagcctatg ctttacggcg atttctttaa agacgtgatt ttcgtcaata
    25741 ccgacgcgca tccgaccatg cacatcatga aggaagagtt ccacggcgca ttggcaatgg
    25801 tgtcgcacag cttgacatcg cctgttttgt atttggcggc tgccggtgtt gccgccgcat
    25861 ggcttttgta cgtcaaactg ccgcacctgc cagcgaaaat tgcacagacg ttccgtccga
    25921 tttacgtttt gtttgaaaac aaatactacc tcgacgccct gtatttcaac gttttcgcca
    25981 aaggcacacg cgcattgggt accttcttct ggaaagtcgg cgataccgcc attatcgaca
    26041 acggcatcgt caacggctct gccaaactgg tcggcgcgat tgccgcgcaa gtgcgtaaag
    26101 ctcaaaccgg ctttatctat acctacgccg ccgctatggt gttcggcgta ttggtactgc
    26161 tcggcatgac cttctggggc ttgttcagat aaaggccggc ttcagacgac ctgttcatgg
    26221 ttgtgtagag gtcgtctgaa aaaagaaact tgagaaacaa tgtcaaacac acaaaccatc
    26281 cgctctgccg acttcaccgc ttcccgcgca tggggtgcac tcgacatcgc caatatgaac
    26341 ggcaccaccg tgcgcctgca ctggacggac cagtcctaca aatggcacgt caacgacggc
    26401 gaggaagtgt ttgccgtgat gaacggcgaa gtcgatatgc actatcgaga aaacggcgaa
    26461 gaacacatcg ttcggttgaa aagcggcgac atcttctacg caggaattgg cacggaacac
    26521 gtcgcccatc cgtgcggcga agcgcggatt ttggtgattg agaaagaagg cagtgcttga
    26581 ggtcgtctga aaagagggtt tcaggctgca atggaattgt aggttgaggt aagaagagat
    26641 gacgcagtta tttttacatt cagatacacc aaatgagaag gaactgattt caatatatga
    26701 gaaagctgtt tcagaggcag tagagctgta tattttctct gcgtatctca ctcattggga
    26761 tgtaaaaaat aagcttaata gcaaattaca agagttcaaa tttatttttg ggcaggattt
    26821 cggattgtct aaaaaagagg ctattcagga agtattgaaa tggcttccgt cccatttgaa
    26881 atttacattg atggtagctc aaggcattca gggttttcat cctaaagctg tattttggaa
    26941 gaatgataag aatgaatatt atgctttaat cggttcttca aatttgactc atgctgcgtt
    27001 taatagcaat tatgaagcaa atattttgac taaaatttct gagcaggatt ttataaaagc
    27061 taaatcttgg gctaatgaaa ttgtgatgaa atctatcccg gtatcggaag attggcttga
    27121 ggagtatcag gaagctgaaa ttaactataa gaaatcaact gttagacaat cagtgatgga
    27181 taagttattt atggagatgc caaattataa tcaagaactg attgcggcac ggagaaagca
    27241 gatgagaaac catcaaactg tgtgtaatca attaaaaaat cttattaagc aatgtgcagc
    27301 aggaaaaata gacaataatg atttttatgg ggaatttaat aaattatggt cttggaaaag
    27361 cgaaaataaa ggagaagggg taggcaatcg ttttcaagat aaaacgtgga aaaggacggg
    27421 taagtcttca gattttagaa aattatgcat tgccatccaa tcagtttttg atgctccact
    27481 tactgagaga gataatgtgg ttgccaagca aattgattgg ctaaaagaat gtggggtttc
    27541 cactcgtggt tcagtttttt ctgaaatgct ttgtcaagaa tatccagata gatatcctgt
    27601 tttaaatgct cccattaaaa aatttttaga agaaaataaa tttaagtctg caaaaggagc
    27661 aagcgaaggc tccaaatata ttgatttaag tatgaagtta agagctttac tagctatgca
    27721 gtcagaaata aaagatttgg ctgaattaga tgttttagta caggcagaat atcgcaatcg
    27781 tacagatatt gattgggaat aagtaaagaa ggggggggag tattcattcc ttctttcaga
    27841 cgacttccta aaacgctgtc tgtaaagacc aacccataca actatatttt ttattttaac
    27901 cacaggttaa ccactatgtt ttccaactac ctactcagct tggcaatatg gatacccatc
    27961 gttgcgggcg tgctggtttt ggcgacgggg ccggacagcc gtgcgccgtt tgcccgcgtg
    28021 ctcgctttaa tcggtgcgct tgccggtttc ttggtaacgc tgcccctgtt taccggtttc
    28081 gaccgtttga gcggcggcta tcagtttacc gagttccacg agtggattcc gcttctgaaa
    28141 atcaactacg cattgggcgt ggacggtatt tcagtgctct ttatcatctt gaatgcgttt
    28201 attacgctgt tggtggtatt ggccggttgg gaagtcattc agaagcgtcc ggcgcagtat
    28261 atggcggcat tcctgatgat gtcgggtttg attaacggtg cgtttgccgc gcaggatgcg
    28321 attctgtttt atgtgttctt cgagggtatg ctgattccgc tgtacctgat tatcggtgta
    28381 tggggcggcc cgcgccgcgt ctatgcgtcg gtcaagctgt tcctctatac gctgatgggt
    28441 tcgctcctga tgctggtggc gatggtttac ctttactatc aaacaggcag cttctctatt
    28501 gtcgatttcc aaaacatcaa acagcttccg ttgggcgtgc aacagctttt gtttgcggcg
    28561 ttcttcctgt catttgccgt gaaagtgccg atgttccccg tgcacacttg gctgccggat
    28621 gcccacgttg aagcaccgac cggcggttcg atggtgttgg cggcaattac gctgaaactg
    28681 ggtgcgtatg gtttcttgcg ctttatcctg ccgattatgc cggatgcggc tcgctatttt
    28741 gcccccgtga tcatcgtatt gagcctgatt gccgtaattt atatcggtat ggtggccctg
    28801 gtgcaaaccg atatgaaaaa actggtggcg tattcgtcca tcagccatat gggttttgta
    28861 acgcttggga tgtttttgtt tgttgacggg cagttggacg actgggcatt gaaaggtgca
    28921 atcattcaaa tgatttccca cggtttcgtg tctgccgcga tgtttatgtg tatcggcgtg
    28981 atgtacgacc gcctgcacac gcgcaatatt gccgattatg gcggcgtggt caatgtgatg
    29041 cccaagtttg cggcgtttat gatgctgttc ggtatggcga acgccggtct gcctgcgact
    29101 tccggcttcg tgggcgagtt tatggtgatt atgggcgcgg tcaaagtgaa tttctgggtc
    29161 ggcgcgctgg cggccatgac cctgatttac ggtgcatctt ataccctgtg gatgtacaaa
    29221 cgcgttattt ttggtgcgat ccacaatccg cacgttgccg aaatgcaaga catcaattgc
    29281 cgcgaatttg cgattttggc ggtgttggcg gttgctgttt tgggtatggg cctgtatccg
    29341 aacgcattta tcgaagtggt gcatcaggcg gcaaacgatt tgattgccca tgtggcacaa
    29401 agcaagattt gaggtgtgta aatgaattgg tctgatttga atttaatgcc cgcactgccc
    29461 gaagtcgcgc tgctgtcgct gctggtgtta ttgttgctgg cggacttgtg ggtcagtgat
    29521 gacaaacgcc ggtggacgca ttacggcgcg ttggcaacgg tggcggttac ggctgtggtg
    29581 cagcttgccg tttgggaaca gggcagcacg tcttcgttca acggcatgta tattgcagac
    29641 ggtatgtcgc gtttggcaaa aatggtttta tatgccttga cgtttgccct gtttgtctat
    29701 gccagaccct acaaccaagt gcgcggtatt ttcaaaggcg agttttacac cctgtcgctg
    29761 tttgccctct tgggcatgag cgtgatggtg agcgcggggc attttttaac tgcctatatc
    29821 ggtttggaac tcttgtcgct tgccctttac gccctgattg ccctgcgccg cgattccagc
    29881 tttgccgccg aagccgcctt gaaatatttt gttttgggcg cgctggcatc cggcctgctg
    29941 ctctacggta tttctatggt ttacggcgca accggttcgc tggaatttgc cggcgtgctc
    30001 gcctcttcct tcaatgaaga agcaaacgaa tggctgttga aactgggtct ggtgtttatc
    30061 gtcgtcgccg ttgcgttcaa actcggcgcg gtgccgttcc atatgtgggt gcccgacgtg
    30121 tatcacggcg cgcccacttc tgttaccgcc ctggtcggca ctgccccgaa aatcgccgcc
    30181 gttgttttcg ctttccgcat cctcgttacc gggttgggaa ccgtgcatca tgactggtct
    30241 ctgatgtttg ccctgcttgc cgccgcctcg ctgctggtcg gcaaccttgc cgccatcatg
    30301 cagaccaata tcaaacgtat gctcgcctat tccaccgtat cgcatatggg gtttatcctg
    30361 ctggcgttta tggcgggcgc ggtcggcttt gcggcgggtc tctattacgc cattacctac
    30421 gcgctgatgg cggcggcagg gttcggtgtg ttgatggtgt tgtcggacgg ggacaacgag
    30481 tgcgaaaaca tcagcgattt ggcagggttg aaccaacacc gcgtatggct tgcctttttg
    30541 atgctgctgg ttatgttctc tatggcgggc attccgccgc tgatgggctt ttacgccaaa
    30601 ttcggcgtga ttatggcgct cttgaaacaa ggccatgttt ggttgtccgt atttgccgtc
    30661 gtgatgtcgc tgattggtgc gttctactac ctgcgcgtgg tcaaagtcat gtattttgac
    30721 gaatccggcc gcgcccgtcc caccgtcggc ggcaacaatg ccgcaaaatt ccttttgagc
    30781 gtcaacgcgc tgctgttggt gctgtggggt atcatgccgc aaaccgttat cgactggtgt
    30841 gccaaagcat tggaaaacac gctgtaagcc gccgcaacgg cagccgtgtc agaggctgcc
    30901 gtttttgtta agatatgccg ttccgcaatg cagttcagac ggcatcgctg ccgacaacgc
    30961 ccaaacagaa agcccaccat gaccgcatcc atgtacatcc ttttggtctt ggcactcatc
    31021 tttgccaacg tcccctttct cacgaccaga ctgttcggcg tggccgcgct caagcgcaaa
    31081 catttcggac accacttgat cgagctggcg gcaggtttcg cgctgaccgc cgttcttgcc
    31141 tacatcctcg aatcccgtgt gggagcggta cacaatcagg gttgggagtt ctacgccacc
    31201 gtcgtctgcc tgtacctgat ttttgcgttt ccctgtttcg tgtggcggta tttttggcac
    31261 acgcgcaaca gggaatagac aagcatagaa atgccgtctg aaaccttttc agacggcatt
    31321 tgtttcattc aagtgcaggc cggcatcgct gtgccggcac gtttcagccg gcgatatacg
    31381 ccggttttaa tatttgcggg cgactgcaaa ttctgccaac tgccgcaggc gcagggcttt
    31441 gtcgccgaag ggttcgagca gcgcgaccgc ttcggcaacc agtttgtgtg cgtatgagcg
    31501 cgccgcttcc aagcccatca gtctcacata agtcggcttg tcgttgtctg cgtctttgcc
    31561 cgccgttttg cccaaaatcg ccgtgtccgc ttcacaatcc aacacatcgt caatgacttg
    31621 gaacgccagc cccagttttg ccgcataatc gtccaatacc gccagactgt cttcagacag
    31681 attcgggcag gacatcgccc ccaataaaac cgccgcacgg attagcgcac ccgttttcag
    31741 gctgtgcatc tgttccaaat cggcttgaac catttgtttg ccgacatttg ccaaatcgat
    31801 tgcctgaccg cccgccatgc ccgcgctgcc gcccgctttc gccagcaccg acaacattgc
    31861 caactggcgt gcggcgggca gttctgtcgg acggctcaat acgtcgaagg cttgggtttg
    31921 taaagcatcg ccggtcagaa gggcgtttgc ttcgccatat ttgatgtggc aggtcggttt
    31981 gccccggcgc aggctgtcgt tgtccatcgc cggcatatcg tcgtgaacca gcgaataggc
    32041 gtgaatcatc tcgattgccg ccattgcctg ttctactgct tcatgcacgg cttcgcctaa
    32101 ttccgaagcc gccagcacca gcatcggacg cagacgcttg ccgccgtcca aagccgcata
    32161 acgcatcgct tcgtgcagcg tgtgcgggat ttcgttttca gacggcaaaa agcgttccag
    32221 cagcagctct gtctgcgcct gcgccctaag ctgccatgct ttcaaatcat tcgtcggatt
    32281 caaggttcaa ctccttcggt ccgtccgcat ccaaaacctg taatttctgt tcgacttgtg
    32341 ccagcttggt ttgacagtat ctgaccagct cgttgccttc ctgataggcg gcaagcgcgt
    32401 cttccaaggg catttcgccc tgcatagact gcgtcagcga ttcaaggcgc gacagtgcct
    32461 cttcaaacga tttcggggcg tttttcttca tagtttcggg tgttccggta aaaagcggat
    32521 tgtagcattt ttgcaaaacg gatgccgtct gaaacccgaa tccggcttca gacggcattg
    32581 ttttgtcctg caaacggcaa ggcgttaccc gggcagttcg tcggtgatgc cctgcaaaaa
    32641 ggcgaggcgt tcggggcttg ccgccctggt ttcggcggcg gctttgaagg cgcagccggg
    32701 ttcggcgcgg tgggtgcagt tgtggaagcg gcattgtccg acaaggtggc ggaaatcggg
    32761 gaaatagcgc ggcaaatcgg cggcttggag gtggtgtaaa ccaaattctt gcaaacccgg
    32821 ggagtcgatg agttgggttt cgccgttcaa atcataaagc cgggcgtggg tggtggtgtg
    32881 ttttcccgag tcgagtgcgg cggaaatgtc gccggtgcgg gcggtttggc tgcccaaaag
    32941 ggcgttggtc agggtggatt tgcccatacc gctctgcccg agcaggatgt tgctgtgccc
    33001 ttgcagggcg gggcgcaggc tgccggcgtt ttccagtgcg tgggtttcga tgacgggata
    33061 acctagcgtt tcgtagaatt tgagtttttc gcgccaaagg gcggtttcgg gcaggtcggc
    33121 tttgttcagg acgatgacgg cgcggatgcc ggcggcttcg gcggcaagca gggcgcgctg
    33181 tagcagccgc acgctcggac tcgggacggc ggcggttacg atgaggagtt gggtaacgtt
    33241 ggcggcaatg agtttggttt tccacgcgtc ttggcggtag agcaggcttt ggcgcggtaa
    33301 aaaatcttca atcacaactt gttcggcgtt gacggggctg atgcggacgc ggtcaccgca
    33361 ggcgaaatcg acgcgttttt tgcgggtgct ggcttcgtag gttgtgccgt cgggcgtgcg
    33421 gacgatgtag cggcggccgt agctggcggt aatttgggcg gtgtcgttca tggtttcttt
    33481 ggggttgggt gtggaaatgc cgtctgaaaa cgggtgttcg gacggcatcg gttcagtcgt
    33541 gctgccactc gacgtgttcg ttgaggaagc cgccgctctg gtgcgcccag agtttggcgt
    33601 aaagcccgcg tttttcgagg agttcggcgt gtgtgccttc ttcgatgatg cggcctttgt
    33661 cgaggacgac gagcctgtcc attgcggcga tggtggagag gcggtgggcg atggcgatga
    33721 cggttttgcc gtccatcatt ttgtcgaggc tttcctggat ggcggcttcg acttcggaat
    33781 cgagcgcgct ggtggcttcg tccaaaagaa gaatcggcgc gtctttgagc atcacgcggg
    33841 caatggcgat gcgctggcgt tgcccgccgg agagtttcac gccgcgttcg ccgacgtgcg
    33901 cgtcatagcc gcgccgccct ttggcatcgg aaagttcggg gatgaagccg gcggcttcgg
    33961 cgcgttcggc ggcggaaacc atttcggcat cggtcgcgtc ggggcggccg taaataatgt
    34021 tgtcgcgcac ggaacggtgc agcagcgagg tatcttgcgt gaccaaaccg atttgggcgc
    34081 gtaaagattc ttgggtgatg ccgcttatgt cctgcccgtc gatcgaaacc gtgccgcttt
    34141 gcggttcgta gaagcgcaaa agcaggttga cgatggtgga tttgcccgcg ccgctgcgtc
    34201 cgatcaagcc gaccttttcg ccgggtcgga tggtgaggtt gaagccgttg agcagcggtt
    34261 tgcccgcttc gtaggagaaa tcgacgtgtt caaatttgat tgcgccttgc ggcactttca
    34321 acggcagggc gttcggcttg tcgaggatgg tgtgcggttt ggacagggtt gccatgccgt
    34381 cgccgacggt gccgatgttt tcaaacagcc gcgcggattc ccacataatg tattgcgaca
    34441 aaccgttgac gcgcaacgcc atggcggtgg ctgtagcaac cgcgcccacg ccgacctgcc
    34501 cgttgtgcca gagccagatg cccagtgcgg cggtggagag ggtcagggag gtgttgacga
    34561 tgaagctgca cgaatgcagc agcgtcgcca gccgcatttg ggcgcgcacc gtaaccataa
    34621 attcttccat cgactgcttg gcataggcgg cttcacgcgc gccgtgggag aagagtttga
    34681 cggtggcgat attggaatag gcatcggtaa tgcggccggt catcagcgag cgggcatccg
    34741 cctgccatgc ggcggtttgc cccaatttgg gaatcagcag gcgcatcacc gaagcgaaac
    34801 cgacaatcca gccgataaag ggcagcagca gccatgagtc gagcgaggcg agaatcacgc
    34861 cggaggtaat gaaatacacc gacacataaa cgaccatatc ggcaaccgtc atcaccgcgt
    34921 cgcgcaacgc cagcgcggtc tgcatgactt tggcggacac gcgtccggca aattcgtcct
    34981 gataaaaacc gaggctttgg ttcagcatca ggcggtggaa gttccagcgc aggcgcatag
    35041 ggaacacgcc ctgaagggtt tgcaggcgca cgttggacgc ggcaaacgcc cacgcaaccg
    35101 aaaacaccat catcgccgcc attgccgcca gttcccaacc tttttcggca aacagttcgg
    35161 tgggcgcgta tttgccgagc cactccacga ttttgcccat aaattgaaaa accagggctt
    35221 ccataatgcc gatgccggcg gtcagcgcag ccagggcggc tatccatttc cgcacgccgg
    35281 tcatgctgct ccagacaaac cgccacaagc ctttttctgg cgttttcggg gcggcttcgg
    35341 gataagggtc gattcgggac tcgaaccagg aaaatatttt gttcaacatt gttttcgatt
    35401 tcggtaaaac agtttcagac ggcatcaaac acaatgccgt ctgaaaggaa ggacaataac
    35461 gccattttac gggaaaagcc gtcgggaaga cagcgcgggg cggaaacgca gggtttcgtc
    35521 agggcaaacg ccgcgccgcc ttcagacggc attatttcag caggtttttc aaagcaaggc
    35581 gcacgccttc gcccacgtcc gtcccctccg gaacgccttt gaccgccgct tttgcttcgc
    35641 gttcgctgta acccagcgca agcagcgtgc tgacgatgtc ttccgtttcg tcggcggcgg
    35701 gtgcggcggc aaacagcccg tccgttaccg tatgcgcgac cagcttgccg cgcagttcca
    35761 agaccatacg ttcggcggtt tttttgccga ttcccggcgc ggaagacagg cgtttgacat
    35821 cttcttctgc aaccgcctgc gccagctcgt ctgccgtcat cgccgacaaa atgcccaaag
    35881 cggtttttgc cccgatgccg ctgaccttga tcagttggcg gaatgttttg cgctcttccg
    35941 cagtggcaaa accaaataaa agatgtgcgt cttcccgaat gataagctgg gtaaacagtt
    36001 gtacgctttc acccacgggc ggcaggttgt agaaggtctg catcgatacg tcggcctcat
    36061 agccgacacc gttgacatcg atgacgattt gcggagggtt tttttcaacc agtttgccgg
    36121 tcagtctgct gatcatgtgt gccgaatcct gaagtgtcgg gtgcaaaatg ccgtctgaaa
    36181 ccggttcggg cttcagacgg cacggattgt atcaaattca gtcgtcgcgg cgggaggaaa
    36241 tcacgcggcc ggtacgggca tcgacaacga ctttgtattc ctgtccgttt ttgacgattt
    36301 cgacatcata gtgcggacgg ccgttgtcgt gttcgagatc gatgtcggtg attttgccgc
    36361 cgacacgcgc caacgctgct ttttcggctt gggcgcggct gatgattttg tcttgtttgt
    36421 tgtgttggtg tgcggcgtgt ccgtggtcgt catcgccgtg tccgtcgtgg tgggcgagtg
    36481 cgggggcgga aatgcccatc agtgcggttg cggcggcggt caagagaagg cgtttgaagt
    36541 tcatgttttg ctcctgcgaa gtatctggtg gtgtttgaaa gacgtaaagg cgggacatca
    36601 accggcggtc aataccgtct gaatcatacc gcgcgcctga gcttcggcct cggcggcgcg
    36661 ttcctgcgag gcaaacggac ccattttgac gacgtattcg taacggcgtt tttcaatggc
    36721 gaggttcggg cttgatgtcg aagcggcgaa gttttgggcg gcttggttca gataggcttg
    36781 tgcttcgtgt tccgtaccga aagatttcaa gtcgataaag atgtctttgt tttcggcaac
    36841 cggtgtggat tggcccggga cgatttgttc gattttgacg tgtgccgtcc cttggccgac
    36901 aaagcccaat ttttgcgcgg cggctttgga tacgtcgatg atgcggttgc cgtggaaggg
    36961 accgcggtcg ttgacgcgga cgatgacgct tttgccgttt ttggtattgg ttacgcgcac
    37021 atagctgggg atgggcaggg ttttgtgggc ggcggtaaag gcgttcatat cgtatcgttc
    37081 tccgccggaa gttttgcgcc cgtgaaacct gccgccgtac cacgaggcgt tgccggtttg
    37141 cgtgaattcg gcgacttggt ttttcggcgt gtagcgtttt ccggcgactt tgtagctgcg
    37201 gttggcggag gcgtgcagtt tttctgcctt gaccactgcg tcggcggatg ccgtctgaag
    37261 ggagtgtgtg ccgaatgcgg cggtgagaag gaaaagggtt tttcgggtta aagtcaaaac
    37321 gtgttccgtt cttgagttga agacgaatgg gcatcatgcc cgccggatac gttccgaacc
    37381 gccgtacagt gcggacggcg gttcggaatg tgtccggata ggttttcaga cggcatgaac
    37441 ttgcgttcaa acgccgcctg cgtaaccgtg ttgccgccac gcttcaaaga gaatcacggc
    37501 aacggtattg gaaaggttca tactccgact gccgggctgc atgggcaggc ggattttttg
    37561 cgcggcgggc aggctgtcga ggatgtcggc aggcagtccg cgcgtttccg gcccgaacag
    37621 taaaacgtcg cctttttgaa acgcggtttc atcggggcgc gccgtgcctt tggtggtcag
    37681 ggcgaaaatg cgcctgcctg cgagtgcctt gaggcagtcg tcgaagtttt cgtacaccgt
    37741 caggctggcg aattcgtggt agtcgagccc ggcgcgtttc attttggcgg aatccaatgg
    37801 gaagccgagc ggtttgacaa ggtgcaaatc cgcgccggta ttggcgcaca ggcggatgat
    37861 gttgcccgtg ttcggcggga tttccggctg gtataaaacg atggtaaaca taaatatcaa
    37921 tcacttatag gcgcgtaacc ttgccacaag gcggatgggg tgtcaaaaaa tttagttatt
    37981 ttttcattgg cgtgcgtgcc agcgtccagc agcagattcg gtttgcgccc gattttttca
    38041 gcgtctttgc caattcgtcc agcgtcgcgc cggtggtaaa gacatcgtcg attaacagaa
    38101 tattacagtt ttccggtatc ggtgtgcgga tttcaaaggc gtttttgatg tttcgccgcc
    38161 gttcgccgcc tttgagcgtg ctttgcggcg ggcggtggtg tcggaaaacg gtgtgtcggg
    38221 gcagtatctg ccagccgtag cgttgtgcca gcagcccgac gatgctttca ctttggttga
    38281 acccgcgttg cagcagccgc tccctgctta gcggtacggg caggacgaaa tcgaaacatt
    38341 cgtctgtaag ccggtcgggc ggattctgca tcatcaggtc tgccagcggc tgcaccatgc
    38401 tcaaatcagc caagtgcttc agcgcgtgta tcatattgct gacgggcggt tcgtaatgca
    38461 gcgaagccca catccggtcg aatgcgggcg gttttttctg acagccgccg cacaccgcgc
    38521 cgccttggat gtgtctgaaa cacagggggc agctgtttgc cgcgtcggtg cggtatgccg
    38581 ccaaatccgc cgcgcagccg gcgcagatgc cgtctgaaac gccggacgaa ccgtggcata
    38641 atacgcaacg cctgatagtg ggcgcgtctg cgatgcgccg ccaacaagag agaaaatcca
    38701 tgcctgatgc cgtcaaaaaa gtttacctga tacacggttg gggggcgaac cgccacgtgt
    38761 tcgacgattt gatgccgcgc ctgcctgcaa cgtggccggt gtccgccgtc gatttgcccg
    38821 gacacggcga cgcgcccttc gcgcaaccct tcgatattga ggcagccgcc gacgctgttg
    38881 ccgcacaaat cgatacgccc gccgacattc tcggctggtc gctcggcgga ttggtcgcgc
    38941 tgtatctggc ggcgcgccat cccgacaaag tccgttcgct ctgcctgacg gcgagtttcg
    39001 cacggctgac ggctgccgaa gattatcccg aagggcttgc cgcgcctgca ttgggcaaaa
    39061 tggtcggcgc gttccgaacg gattatgcca aacatatcaa acagtttcta caactccagc
    39121 ttttgcacac gcatgatgcg gacagaatca taggcagaat cctgcccgat ttggcgcgct
    39181 gcggcacgcc ctccgcctta caggaagctt tggacgcggc ggaaagggcg gatgcgcggc
    39241 atttgttgga caagatagat gttccggtac tgctggtgtt cggcggcaaa gacgcgatta
    39301 caccgccgcg tatgggtgag tatctgcatc ggcatttgaa aggaagcagg ctggttgtga
    39361 tggaaaaggc ggcacacgcg ccgtttttga gccatgcgga agcgtttgcc gcgctgtacc
    39421 gcgactttgt cgaaggggtt tgacatgaat ccgcaggaca aatgttggca ggttcaccgc
    39481 catcttgccg aacatgccga ccaacggctg acactcgtcc gcaacgcgcc caaacatatc
    39541 ctgcttgccg gggcggatgc ggacatcagc cgcagcctgc tggcgaaacg atatccgcag
    39601 gcggtatttg aagaatacga ttcccgtgcg gattttttgg cggctgccgc agccgtgcgc
    39661 aagggcggtt tttggcaaag gtttacgggt aagggcgtgg tgcaacactg ccaatccccg
    39721 accgcgccgc tgcccgaagc gtgtgccgat atgttgtggt cgaatctcgg actgttggcg
    39781 gcggaacaaa tccttcctgt gctgcacaac tgggcgcgcg cgctggagac ggacgggctg
    39841 ctgtttttca cctgcttcgg gcgagatacc ttggcggaac tgaaatgccg tctgaaagaa
    39901 aatggcattg aaagccgcag cgcgcttttc cccgatatgc acgacttggg cgatatgctt
    39961 gctgaaaacg ggttttacga ccccgttacc gatacggcga agctggtgtt ggattacaaa
    40021 aaggcggaaa cgttttgggc ggatatggac acgctgggcg tttggcgggc gatgtcgtgg
    40081 aacgatgaaa acgccgcgcg ttcgtgtgtc gggacaatat ttgaacggga aggcggtttg
    40141 agtattacgc tggaaacagt gtacggacac gccgttaaaa aactgatgct gccgcaaggg
    40201 gagaacgtgg tgcagttttt tccgaagaga tgatgtgcag atgccgtctg aagctgtttc
    40261 caagtttcag acggcatctg tctgtgaaaa ccgatagaaa taaaggaaat gccgatgtat
    40321 gccgatgccg gagacgtata ttgcgtctat aacttcagac tgaagcagta cactgcctgc
    40381 caggttaccc gagttgaaga acacggtggg aaaaaagcat atgcaaccct gctggctttg
    40441 gactggcagg gcaacaaacc gcttggggcg gcggagctgg cggatttgaa accgctttac
    40501 aaagacttta tgtattggga acgcggtctg cacatgtata aggcaagtgc cgtcgtgccg
    40561 acgggatatg tacgggttgg gaataccgcg ccgctggtcg gcgaagacac gcaacggtat
    40621 gcctcttttt ggggcgacgg ctacgacgtg taccgtcagt tgagatggca gcagataccc
    40681 gaaaaacaga gaaaggcatt caaaaaagcc gccaaaagca aaaagaccgt gatgtttgcc
    40741 ggacgggaat acggcatatc caaacagaat ttgagcgatg tttgggatga ttttgaagac
    40801 gcgatggaac tgaaggcgtt tccctgcctg tcttcgctgt ttctgaccaa atggcataaa
    40861 aatctatatg atagtggatt aacaaaaacc agtacagcgt tg
//
LOCUS       DAWFLT010000010        38989 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWFLT010000010 DAWFLT010000000
VERSION     DAWFLT010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38989
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.010"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..69
                     /locus_tag="WGR74_000482"
     CDS             <1..69
                     /locus_tag="WGR74_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0904967.1"
                     /translation="LRFAIREGGRTVGAGVVSSVIA"
     gene            87..398
                     /gene="rpsJ"
                     /locus_tag="WGR74_000483"
     CDS             87..398
                     /gene="rpsJ"
                     /locus_tag="WGR74_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEZ0904968.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            689..1741
                     /locus_tag="WGR74_000484"
     CDS             689..1741
                     /locus_tag="WGR74_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HEZ0904969.1"
                     /translation="MFYSQVNKGKMMSLLNVFKDILNNKELNSEERHDLERAVKILQD
                     THVNILITGATGCGKSSTINALFSIDQATGKSDGLVKEVAKVGVGVDPETMDIAKYEL
                     ENMTIWDSPGLGDGEKADQKHRRNIINKLLEKDSNGNAVIDLVLVLLDGGSRDLGTSY
                     ELINNVIINTLKKSGEGRTDRLLVAINQCDMAMKGRNWDAEAGRPNAALENFLEEKVC
                     STRKRIQEATGVTVNPIYFSAGFKSEDEEQRPYNITRLLRYIITAMPSEKRVIVAEKI
                     NVEEIERDTSRRKQEERQEIDSSIGSALEKILGAAGGVVKDVVSTIASGVGTLIGGAA
                     KAIGGAISTVASFFGW"
     gene            1750..2433
                     /locus_tag="WGR74_000485"
     CDS             1750..2433
                     /locus_tag="WGR74_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HEZ0904970.1"
                     /translation="MHRYRISEIGQKVTAAGFRHLDVLLVGATGVGKSSTLNALFGEQ
                     KAKVGTGVDPETQLVESYMLNDVLRFWDSAGLGDGKEADRAHRQKLIDVLSKTYTHSD
                     GQWGWIDLVFVILDGSSRDLGTAYDLLRDVILKMIDPDRVIVAINQADMAMKGRYWDK
                     VLHQPQPNLQQFLDEKAESVQKRILEATGLQISKPVYYSAYENYHLKEIMDAVINHIP
                     VCRRKMHTP"
     gene            2652..3296
                     /gene="rplC"
                     /locus_tag="WGR74_000486"
     CDS             2652..3296
                     /gene="rplC"
                     /locus_tag="WGR74_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ0904971.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            3296..3916
                     /gene="rplD"
                     /locus_tag="WGR74_000487"
     CDS             3296..3916
                     /gene="rplD"
                     /locus_tag="WGR74_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ0904972.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            3913..4227
                     /gene="rplW"
                     /locus_tag="WGR74_000488"
     CDS             3913..4227
                     /gene="rplW"
                     /locus_tag="WGR74_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ0904973.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            4233..5066
                     /gene="rplB"
                     /locus_tag="WGR74_000489"
     CDS             4233..5066
                     /gene="rplB"
                     /locus_tag="WGR74_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ0904974.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            5073..5351
                     /gene="rpsS"
                     /locus_tag="WGR74_000490"
     CDS             5073..5351
                     /gene="rpsS"
                     /locus_tag="WGR74_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ0904975.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            5360..5689
                     /gene="rplV"
                     /locus_tag="WGR74_000491"
     CDS             5360..5689
                     /gene="rplV"
                     /locus_tag="WGR74_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ0904976.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            5699..6391
                     /gene="rpsC"
                     /locus_tag="WGR74_000492"
     CDS             5699..6391
                     /gene="rpsC"
                     /locus_tag="WGR74_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ0904977.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            6375..6791
                     /gene="rplP"
                     /locus_tag="WGR74_000493"
     CDS             6375..6791
                     /gene="rplP"
                     /locus_tag="WGR74_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ0904978.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            6791..6982
                     /gene="rpmC"
                     /locus_tag="WGR74_000494"
     CDS             6791..6982
                     /gene="rpmC"
                     /locus_tag="WGR74_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ0904979.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            6982..7245
                     /gene="rpsQ"
                     /locus_tag="WGR74_000495"
     CDS             6982..7245
                     /gene="rpsQ"
                     /locus_tag="WGR74_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ0904980.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            7467..7835
                     /gene="rplN"
                     /locus_tag="WGR74_000496"
     CDS             7467..7835
                     /gene="rplN"
                     /locus_tag="WGR74_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ0904981.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            7847..8170
                     /gene="rplX"
                     /locus_tag="WGR74_000497"
     CDS             7847..8170
                     /gene="rplX"
                     /locus_tag="WGR74_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ0904982.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            8180..8719
                     /gene="rplE"
                     /locus_tag="WGR74_000498"
     CDS             8180..8719
                     /gene="rplE"
                     /locus_tag="WGR74_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ0904983.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAI
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            8722..9027
                     /gene="rpsN"
                     /locus_tag="WGR74_000499"
     CDS             8722..9027
                     /gene="rpsN"
                     /locus_tag="WGR74_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ0904984.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            9043..9435
                     /gene="rpsH"
                     /locus_tag="WGR74_000500"
     CDS             9043..9435
                     /gene="rpsH"
                     /locus_tag="WGR74_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ0904985.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            9449..9982
                     /gene="rplF"
                     /locus_tag="WGR74_000501"
     CDS             9449..9982
                     /gene="rplF"
                     /locus_tag="WGR74_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ0904986.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLAFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            9996..10349
                     /gene="rplR"
                     /locus_tag="WGR74_000502"
     CDS             9996..10349
                     /gene="rplR"
                     /locus_tag="WGR74_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ0904987.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            10368..10886
                     /gene="rpsE"
                     /locus_tag="WGR74_000503"
     CDS             10368..10886
                     /gene="rpsE"
                     /locus_tag="WGR74_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ0904988.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            10879..11064
                     /gene="rpmD"
                     /locus_tag="WGR74_000504"
     CDS             10879..11064
                     /gene="rpmD"
                     /locus_tag="WGR74_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ0904989.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            11066..11500
                     /gene="rplO"
                     /locus_tag="WGR74_000505"
     CDS             11066..11500
                     /gene="rplO"
                     /locus_tag="WGR74_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ0904990.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIAFGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            11512..12822
                     /gene="secY"
                     /locus_tag="WGR74_000506"
     CDS             11512..12822
                     /gene="secY"
                     /locus_tag="WGR74_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ0904991.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            12827..13045
                     /gene="infA"
                     /locus_tag="WGR74_000507"
     CDS             12827..13045
                     /gene="infA"
                     /locus_tag="WGR74_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ0904992.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            13066..13179
                     /gene="rpmJ"
                     /locus_tag="WGR74_000508"
     CDS             13066..13179
                     /gene="rpmJ"
                     /locus_tag="WGR74_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ0904993.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            13245..13607
                     /gene="rpsM"
                     /locus_tag="WGR74_000509"
     CDS             13245..13607
                     /gene="rpsM"
                     /locus_tag="WGR74_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ0904994.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            13627..14022
                     /gene="rpsK"
                     /locus_tag="WGR74_000510"
     CDS             13627..14022
                     /gene="rpsK"
                     /locus_tag="WGR74_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ0904995.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            14042..14662
                     /gene="rpsD"
                     /locus_tag="WGR74_000511"
     CDS             14042..14662
                     /gene="rpsD"
                     /locus_tag="WGR74_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ0904996.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            14688..15674
                     /gene="rpoA"
                     /locus_tag="WGR74_000512"
     CDS             14688..15674
                     /gene="rpoA"
                     /locus_tag="WGR74_000512"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ0904997.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            15698..16066
                     /gene="rplQ"
                     /locus_tag="WGR74_000513"
     CDS             15698..16066
                     /gene="rplQ"
                     /locus_tag="WGR74_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ0904998.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            16213..16926
                     /gene="minC"
                     /locus_tag="WGR74_000514"
     CDS             16213..16926
                     /gene="minC"
                     /locus_tag="WGR74_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ0904999.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            16955..17770
                     /gene="minD"
                     /locus_tag="WGR74_000515"
     CDS             16955..17770
                     /gene="minD"
                     /locus_tag="WGR74_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ0905000.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            17774..18037
                     /gene="minE"
                     /locus_tag="WGR74_000516"
     CDS             17774..18037
                     /gene="minE"
                     /locus_tag="WGR74_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ0905001.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            18041..18961
                     /gene="oxyR"
                     /locus_tag="WGR74_000517"
     CDS             18041..18961
                     /gene="oxyR"
                     /locus_tag="WGR74_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ0905002.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(18958..19149)
                     /locus_tag="WGR74_000518"
     CDS             complement(18958..19149)
                     /locus_tag="WGR74_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905003.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
     gene            complement(19291..22128)
                     /locus_tag="WGR74_000519"
     CDS             complement(19291..22128)
                     /locus_tag="WGR74_000519"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ0905004.1"
                     /translation="MLDKYNPAEIESKHYQNWESQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTVWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPSEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQQGFALPEKYAGLDRFAARK
                     QMVADLQEQGLLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDETG
                     NVYVARNQAEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLDKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATATEPRGYSFPDMWIVDRL
                     NQTIEQVTQAYETYRFDLAAETLYSFMWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADSGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQTQLSKLKD"
     gene            complement(22225..23034)
                     /gene="zupT"
                     /locus_tag="WGR74_000520"
     CDS             complement(22225..23034)
                     /gene="zupT"
                     /locus_tag="WGR74_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ0905005.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            complement(23215..24471)
                     /locus_tag="WGR74_000521"
     CDS             complement(23215..24471)
                     /locus_tag="WGR74_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ0905006.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAKGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWRMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEGRKKGTLQIFRQAKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     HFNQTISRIDHNGLRIKTVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(24550..25941)
                     /locus_tag="WGR74_000522"
     CDS             complement(24550..25941)
                     /locus_tag="WGR74_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0905007.1"
                     /translation="MQLFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(26298..27074)
                     /gene="lpxA"
                     /locus_tag="WGR74_000523"
     CDS             complement(26298..27074)
                     /gene="lpxA"
                     /locus_tag="WGR74_000523"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ0905008.1"
                     /translation="MTLIHPTAVIDPKAELDSSVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSARGIIR"
     gene            complement(27117..27797)
                     /locus_tag="WGR74_000524"
     CDS             complement(27117..27797)
                     /locus_tag="WGR74_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEZ0905009.1"
                     /translation="MERYKNAVGKDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CLQQAFKDGNWLYNSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDATGKVLMKVFPKKPKSTPSVQHNQKWIFRELS
                     NINNFRNRLAHHEPICFKGAIKDTGYARNIHQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            complement(28108..28557)
                     /gene="fabZ"
                     /locus_tag="WGR74_000525"
     CDS             complement(28108..28557)
                     /gene="fabZ"
                     /locus_tag="WGR74_000525"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ0905010.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            complement(28593..29636)
                     /gene="lpxD"
                     /locus_tag="WGR74_000526"
     CDS             complement(28593..29636)
                     /gene="lpxD"
                     /locus_tag="WGR74_000526"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ0905011.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDTSVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCRLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDGKDTQ"
     gene            complement(29669..30169)
                     /locus_tag="WGR74_000527"
     CDS             complement(29669..30169)
                     /locus_tag="WGR74_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ0905012.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLKDAKKAQAEEKWCGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQDVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(30235..32628)
                     /gene="bamA"
                     /locus_tag="WGR74_000528"
     CDS             complement(30235..32628)
                     /gene="bamA"
                     /locus_tag="WGR74_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ0905013.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(32685..34025)
                     /gene="rseP"
                     /locus_tag="WGR74_000529"
     CDS             complement(32685..34025)
                     /gene="rseP"
                     /locus_tag="WGR74_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ0905014.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLNYERAGQTHT
                     ADIRPDTVEQSDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVIRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(34060..35295)
                     /locus_tag="WGR74_000530"
     CDS             complement(34060..35295)
                     /locus_tag="WGR74_000530"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ0905015.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFQPEYAVVADAEYAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIRSIILTASGGPFLTTDLGTFDRITPA
                     QAVKHPNWRMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGVAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(35300..36097)
                     /locus_tag="WGR74_000531"
     CDS             complement(35300..36097)
                     /locus_tag="WGR74_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ0905016.1"
                     /translation="MLKQRVMTAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSKLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(36100..36846)
                     /locus_tag="WGR74_000532"
     CDS             complement(36100..36846)
                     /locus_tag="WGR74_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ0905017.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVRHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKYLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(36902..37459)
                     /gene="frr"
                     /locus_tag="WGR74_000533"
     CDS             complement(36902..37459)
                     /gene="frr"
                     /locus_tag="WGR74_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ0905018.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(37640..38767)
                     /locus_tag="WGR74_000534"
     CDS             complement(37640..38767)
                     /locus_tag="WGR74_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ0905019.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHAVRLGGAVLFATASARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEQNMVKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(38837..38912)
                     /locus_tag="WGR74_000535"
     tRNA            complement(38837..38912)
                     /locus_tag="WGR74_000535"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38877..38879),aa:Thr,seq:cgt)
BASE COUNT         9363 a         8722 c        10584 g        10320 t
ORIGIN      
        1 ttgcgctttg cgattcgcga aggcggccgt accgtgggtg ccggcgtggt ttcttctgtt
       61 atcgcttaat tgaaggatat tgataaatgg caaaccaaaa aatccgtatc cgcctgaaag
      121 cttatgatta cgccctgatt gaccgttctg cacaagaaat cgttgaaact gcaaaacgta
      181 ccggtgcagt tgtaaaaggc ccgattcctt tgccgaccaa aatcgaacgt ttcaacattc
      241 tgcgttctcc gcacgtgaac aaaacttccc gtgaacaatt ggaaatccgc acccacttgc
      301 gcctgatgga catcgtggat tggaccgaca aaactaccga tgcgctgatg aagctggatt
      361 tgccggccgg tgttgatgta gaaatcaaag tccaataatt agggctgttg aaaatcccca
      421 agcaattaat gcttggggat tttttatgtt atgccgtctg aaaatatgcg gcggcaaaca
      481 gatttaaggc aaaacaacaa aatcaaaaaa cagtgcggtc ggcgaaatcg gcttattttg
      541 tgcctcaaga attttcagac ggcatttttt agtgaacaaa acaggatatt ttttatccat
      601 agccttccga aggcttgccc ccccccgaag aaaggtagaa tcgggcttgg tttgggcaga
      661 agcatccatc tgattctaag gatggatgat gttttattct caagtaaata aaggaaagat
      721 gatgagctta cttaatgtct ttaaagatat tttaaacaac aaagaattga attcggaaga
      781 acggcatgat ttggagcggg cggttaagat cttgcaggat acgcatgtca atattttgat
      841 taccggagcc accggttgcg gaaaatcatc caccatcaat gccttgttca gcatagatca
      901 ggcaacggga aagagtgatg gactagtaaa ggaagtcgca aaagtaggcg tgggtgtaga
      961 tcctgaaact atggatattg ccaaatatga attggagaat atgacgattt gggatagtcc
     1021 gggcttgggc gacggagaga aggcagacca aaagcaccgg cgcaatatca tcaataagct
     1081 gctggagaaa gacagtaacg gcaatgccgt catcgacttg gttttggtgc tgcttgacgg
     1141 cggctccaga gacttgggga catcatacga actgattaac aatgtaatca tcaatacatt
     1201 gaagaagagt ggggaaggcc gtacagaccg gttgctggtt gcgattaatc agtgtgatat
     1261 ggcgatgaag gggcggaatt gggatgcgga agccggccgt ccgaacgccg ccttggaaaa
     1321 ctttttggag gagaaagtgt gttcgacacg caagcgcatt caggaagcta ccggcgttac
     1381 ggtcaatccg atttattttt cagcaggctt taagagtgag gatgaggagc agcgtcccta
     1441 taacataacc cgcctgttgc gttacatcat tacggctatg ccgtctgaaa aacgggtgat
     1501 tgtggcagag aagattaatg tggaagaaat cgaacgagat accagccgcc gcaaacagga
     1561 ggagcggcaa gaaatcgaca gtagtatcgg cagtgcgctg gagaaaatat tgggggctgc
     1621 gggaggcgtg gttaaagatg ttgtttcaac gatagcaagc ggtgtcggga cgctgatcgg
     1681 cggtgctgca aaagcgattg gaggggcgat tagcacggtt gcttcttttt ttggctggta
     1741 ggagcgcata tgcatcgtta ccgtatttct gaaatcgggc aaaaagtcac agcagccggt
     1801 ttccgtcatt tggatgtctt gcttgtcggt gcgacaggtg tcggcaagtc ctcaacctta
     1861 aatgccttgt ttggcgaaca aaaagccaag gtaggcacag gcgtggatcc cgaaacgcaa
     1921 ttggtcgaaa gctatatgct gaacgatgtg ttgcggtttt gggacagcgc aggtttgggc
     1981 gatgggaaag aagccgaccg cgcccatcgg caaaaactga ttgatgtcct gtctaaaacc
     2041 tatactcatt cggatgggca gtggggctgg atagatttgg tgttcgttat ccttgacggc
     2101 agctcccgcg atttgggtac ggcctatgat ttgttgaggg atgttatcct taaaatgatt
     2161 gatccggata gggttattgt tgcaatcaat caggcggata tggcgatgaa aggacgttat
     2221 tgggataagg tgctgcatca gccgcagccg aatttgcaac agtttctaga cgagaaggcc
     2281 gaatcggtac aaaaaaggat tctggaagca accggtttgc agatttccaa acccgtttac
     2341 tattcggcat acgaaaacta ccatctcaaa gaaataatgg atgcagtcat caaccatatc
     2401 ccggtttgtc ggagaaagat gcatacgccg tgatggtcgg ataccgactc ctttaaaagc
     2461 atccgaccct atgccgtctg aagattcaaa acgcttcaga cggcatattg aagatatttt
     2521 tgatattttt gttgatattt ctttgacttg tcagatataa tgtctgactt ggtacattcg
     2581 taccaagttt aactttgtct gaaagacagg ccaatcgtag cctgtccctt tactttaaaa
     2641 ggaaaataat catgacttta ggtctggttg gacgcaaagt tggtatgacc cgcgtgttcg
     2701 acgaacaggg tgtttctgtt ccggtaaccg ttttggatat gtctgccaac cgcgttacac
     2761 aagtaaaatc caaagatact gacggctata ctgccgttca agttaccttt ggtcagaaaa
     2821 aagccaatcg tgtcaacaaa gccgaagccg ggcactttgc aaaagcaggt gttgaagccg
     2881 gtcgcggttt gattgagttt gctttgactg aagaaaaact ggctgaattg aaagctggtg
     2941 acgaaatcac cgtttctatg tttgaagtcg gtcaactggt cgatgtaacc ggtacctcta
     3001 aaggtaaagg tttctccggc acgatcaaac gtcataactt cggtgcccaa cgtacttccc
     3061 acggtaactc ccgttctcac cgtgttccag gctctatcgg tatggcgcaa gacccgggtc
     3121 gcgtgttccc cggtaaacgc atggccggcc aatacggcaa caccaaagca actgttcaaa
     3181 aattggaagt tgtccgtgtt gatgcagaac gccaactgct gttggttaag ggtgctgttc
     3241 cgggtgcggt caacagcgat gttgtagttc gtcccagcgt gaaagtaggt gcgtaatgga
     3301 attgaaagta attgacgcta aaggacaagt ttcaggcagc ctgtctgttt ctgatgcttt
     3361 gttcgcccgc gaatacaatg aagcgttggt tcatcagctg gtaaatgcct acttggcaaa
     3421 cgcccgctct ggtaaccgtg ctcaaaaaac ccgtgccgaa gtaaaacact caaccaaaaa
     3481 accatggcgt caaaaaggta ccggccgcgc ccgttccggt atgacttcat ctccgctgtg
     3541 gcgtaaaggt ggtcgcgcgt tcccgaacaa acccgacgaa aacttcactc aaaaagtaaa
     3601 ccgcaaaatg taccgtgccg gtatggcgac tattctgtcc caattgactc gtgacgagcg
     3661 tttgtttgcg attgaggcgt tgactgctga aactcctaaa accaaagttt ttgctgaaca
     3721 agtgaaaaat ctgggtctgg agcaagtgtt gtttgtaacc aaacagctcg acgagaatgt
     3781 ttacttggct tcacgcaact tgccaaacgt gttggttttg gaagctcaac aagttgatcc
     3841 ttacagcttg ctgcgttaca aaaaagtaat catcactaaa gatgcagttg cacaattaga
     3901 ggagcaatgg gtatgaatca acaacgtttg actcaagtga ttttggcacc tatcgtttct
     3961 gaaaaaagca acgtattggc tgaaaaacgc aaccaaatga cgtttaaagt cttggcaaat
     4021 gcaaccaaac ctgaaatcaa agctgctgtt gagctgctgt ttggtgttca agttgcttct
     4081 gtaactaccg ttacaactaa aggcaaaact aagcgttttg gtcgtacttt gggccgccgc
     4141 agcgatgtta aaaaagctta tgtaagcttg gctgccggtc aagagttgga tttggaagcc
     4201 gctgctgcag ctgcagataa ggaataaaca aaatggcaat cgttaaaatg aagccgacct
     4261 ctgcaggccg tcgcggcatg gttcgcgtgg taacagaagg tttgcacaaa ggtgcacctt
     4321 atgcgccctt gcttgaaaag aaaaattcta ctgccggccg taacaataat ggtcatatca
     4381 ccacccgtca caaaggcggc ggtcataaac accattaccg tgttgtagac tttaaacgta
     4441 acaaagacgg cattcctgcc aaagtagagc gtatcgaata cgatcctaac cgtactgcct
     4501 tcatcgcact gttgtgctat gcagacggcg agcgtcgtta catcattgct cctcgcggta
     4561 ttcaagccgg tgctgtattg gtttccggtg ctgaagctgc catcaaagta ggcaacaccc
     4621 tgccgattcg caacattccc gttggtacga ctatccactg tatcgaaatg aaacccggta
     4681 aaggtgctca aatcgcacgt tctgccggtg cttctgcggt attgctggct aaagaaggcg
     4741 cgtacgctca agtccgtctg cgctctggcg aagtccgtaa aatcaacgta aattgccgtg
     4801 cgaccattgg tgaagttggt aacgaagagc aaagcctgaa aaaaatcggt aaagccggtg
     4861 ccaaccgttg gcgcggtatt cgtccgaccg tacgcggtgt tgtcatgaat cctgtcgatc
     4921 acccgcatgg tggtggtgaa ggccgtacgg gcgaggcccg cgaaccggtc agcccatggg
     4981 gtactcctgc taaaggctac cgcactcgta ataacaaacg cacggataac atgattgttc
     5041 gtcgccgtta ctcaaataaa ggttaattta gtatggctcg ttcattgaaa aaaggcccat
     5101 atgtagacct gcatttgctg aaaaaagtag atgctgctcg cgcaagcaac gacaaacgcc
     5161 cgattaaaac ctggtctcgt cgttctacca ttctgcctga ttttatcggt ctgaccattg
     5221 ctgtgcacaa cggccgcacc catgtgcctg tgtttatcag cgacaatatg gttggtcata
     5281 aattaggcga attctcattg acccgtacct ttaaaggcca cttggccgat aaaaaggcta
     5341 aaaagaaata aggtgaatca tgagagtaaa tgcacaacat aaaaatgccc gtatctctgc
     5401 tcaaaaggct cgtttggtag ctgatttgat tcgtggtaaa gacgttgccc aagctttgaa
     5461 tattttggct ttcagtccta aaaaaggtgc cgagctgatt aaaaaagtat tggagtcagc
     5521 tattgctaat gccgagcaca ataacggtgc ggacattgat gaactgaaag tggtaactat
     5581 ctttgttgac aaaggcccaa gcttgaaacg ttttcaagct cgcgccaaag gtcgcggtaa
     5641 ccgcatcgaa aaacaaactt gtcatatcaa tgtgacagtg ggtaactaag gaaaagctat
     5701 gggacaaaag attaacccta caggctttcg cctggcggta actaaagact gggcttcaaa
     5761 atggtttgct aaaagcaccg acttttctac tgttttgaag caggatatcg atgttcgcaa
     5821 ttatttgcgt caaaaattgg ccaatgcttc ggttggtcga gtggttattg aacgccctgc
     5881 aaaatctgca cgcattacca ttcactccgc tcgtccgggt gtggttatag gtaaaaaagg
     5941 tgaggatatc gaggttttga aacgtgactt gcaagtcttg atgggtgtac ctgttcatgt
     6001 aaatattgaa gagattcgcc gtcctgagtt ggatgctcaa attattgctg acggtattgc
     6061 ccagcagttg gaaaagcgcg ttcaattccg tcgtgctatg aaacgagcaa tgcaaaatgc
     6121 aatgcgttct ggtgctaaag gcattaagat tatgacttca ggccgtctga atggtgcgga
     6181 tattgcccgt agcgaatggt atcgtgaagg tcgcgtgcca ctgcatactt tacgtgcaaa
     6241 tgtagattat gcaaccagcg aagcgcacac cacatatggt gtattgggtc tgaaagtttg
     6301 ggtttatacg gaaggcaata ttaaatcttc caaacctgaa catgagagta aacaaagaaa
     6361 ggcaggtaga cgtaatgctg cagccaacta gactgaaata ccgtaagcaa caaaagggtc
     6421 gcaatactgg catcgctact cgcggtaata aggtaagttt cggtgagttc ggcttgaaag
     6481 ccgtaggtcg tggtcgtttg actgcccgtc aaatcgaagc tgctcgtcgt gcaatgaccc
     6541 gtcatatcaa acgtggtggt cgtatttgga ttcgtgtatt ccctgataaa ccgattactg
     6601 aaaagcctat tcaagttcgt atgggtggcg gtaaaggtaa cgtggaatat tacattgccg
     6661 aaattaaacc aggtaaagtg ttgtatgaaa tggatggcgt tccagaggaa ctggctcgtg
     6721 aagcattcga gttggctgct gccaaattgc ctattcctac aacctttgta gtaagacagg
     6781 tgggtcaata atgaaagcaa atgaattgaa agacaaatcc gttgagcagt tgaatgcaga
     6841 tttgttggac ttgttgaaag ctcagtttgg cttacgtatg caaaacgcta ccggtcaatt
     6901 aggcaaacca agtgaattga aacgtgtacg tcgcgatatt gctcgtatta aaaccgtttt
     6961 aactgaaaaa ggtgctaagt aatgagcgaa actaaaaatg ttcgtacttt gcaaggcaaa
     7021 gtagtaagcg acaaaatgga taaaaccgta acagtattgg ttgagcgtaa agtaaaacat
     7081 ccgctgtatg gtaagattat tcggttatct actaaaatcc atgcccatga tgaaaataat
     7141 caatatggaa ttggtgatgt ggttgttata tcggaatccc gtccattgtc aaaaactaaa
     7201 tcttgggttg tcagtgagct ggttgagaaa gcacgttcta tttaagaatt aaagcaacgt
     7261 gcttggaatg ggaaacgaag tattgcagca aatttaattt gcgtgtaaac ttcgtttcct
     7321 gtctttcagt ttcttctgga agtttcttcc ctttcggggt ccaagactgg tttacttgaa
     7381 ccgcaaggtt tcatttaata agcagcggct ttgctgtaag ttatctgaaa gtggtaaatt
     7441 aagttggtta atttaaaggt aataacatga ttcaaatgca gaccatctta gatgtggctg
     7501 ataactctgg tgcgcgtcgc gtaatgtgta tcaaggtatt gggcggatct aagcgtcgct
     7561 acgcttctgt tggcgatatt attaaagtgg cagttaaaga tgcggctccg cgtggccgtg
     7621 tcaaaaaagg cgatgtatat aatgcggtag ttgttcgtac tgctaagggt gtacgtcgtc
     7681 ctgatggtgc gttaattaaa ttcgataaca atgccgccgt gttactgaat aataaacttg
     7741 aacctttggg tactcgtatc tttggtccgg taacccgtga attgcgtact gagcgattta
     7801 tgaaaatcgt ttcattggca cctgaagtat tataaggaat ggcacgatga ataaaatcat
     7861 taaaggcgat agggttgtag taattgctgg taaggataaa ggtaagcagg gtcaagtagt
     7921 tcgagtgttg ggtgataaag ttgttgttga gggcgttaat gttgtaaaac gccatcaaaa
     7981 acctaatcca atgcgtggca ttgagggcgg tattattact aaagaaatgc ctttggatat
     8041 ttctaatatc gcaatcctga atccggaaac taataaagcg gaccgtgttg gtattaagct
     8101 gattgaaaat gaaggcaaag ttaaacgcgt tcgtttcttc aaatcaaatg gctctatcat
     8161 tggggcataa ggagataaca tggctcggtt gagagagttt tataaagaga cagttgttcc
     8221 tgaattggtt aaacaatttg gttacaaatc agtaatggaa gtcccgcgta ttgaaaaaat
     8281 caccttgaat atgggtgtgg gtgaggctat tgctgataaa aaagttatgg aacatgctgt
     8341 ttccgattta gagaaaattg ccggtcaaaa accggttgtt actgttgccc gtaaatctat
     8401 cgcaggtttt aaaatccgtg ataactatcc ggttggttgc aaagtaacat tgcgtcgtga
     8461 tcaaatgttt gaattcttgg atcgtttgat tactattgca ttacctcgcg tacgtgactt
     8521 ccgtggtgtg agcggtaaat catttgatgg ccgtggcaat tacaatatgg gtgttcgtga
     8581 gcaaattatt tttccggaaa ttgaatacga taaaattgat gctttgcgtg gtttgaatat
     8641 tactattact actacagcaa aaaccgatga ggaagcgaaa gctttattgt cattgtttaa
     8701 atttccgttc aaaggataat catggctaag aaagcactta ttaatcgtga tctgaaacgt
     8761 caagctttgg ctaaaaaata tgcggctaaa cgcgcggcaa ttaaagcggt aatcaatgat
     8821 tcgaatgcaa ctgaggaaga gcgttttgag gctcgtttga ggtttcaatc cattcctcgt
     8881 aatgcggcac ctgtgcgtca acgtcgtcgt tgtgctttga caggtcgccc tcgtggtact
     8941 ttccgtaaat ttggtttggg tcgtattaaa atccgtgaaa tcgccatgcg tggcgaaatt
     9001 ccgggtgttg ttaaagccag ctggtaatag gagtaattaa gaatgagtat gcatgatcct
     9061 atttccgata tgttgactcg tatccgcaat gcgcaacgtg ctaataaagc agcggttgca
     9121 atgccttctt caaaattaaa gtgtgctatt gcaaaggtat tgaaagaaga aggatatatt
     9181 gaggacttcg cagtttcatc tgacgtaaag tctatattgg aaattcaatt aaaatactat
     9241 gcaggtcgtc ctgtaattga acaaatcaag cgtgtatctc gccccggttt gcgtatttat
     9301 aaagcgtcta gtgagattcc aagtgttatg aatggcttgg gtattgctat tgttagtact
     9361 tctaaaggtg taatgactga tcgtaaagca cgttctcaag gtgttggtgg tgagttgtta
     9421 tgcattgtag cctagtggag gaaaagaaat gtcacgtgtc gcaaaaaacc cagtgactgt
     9481 tcccgctggt gtagaagtaa aatttggaac agaggcatta gttattaagg gtaagaacgg
     9541 tgaattgtct tttcctttgc attctgatgt agccattgaa tttaatgatg gcaaattggc
     9601 ttttgttgcg aataacagca gtaaacaagc aaatgcaatg tctggtactg ctcgcgcatt
     9661 agtcagcaat atggttaaag gtgtttcaga aggttttgag aaaagattgc aattgatagg
     9721 tgtgggttat cgtgctcaag cacaaggtaa aatcttgaat ctgtctttgg gtttttctca
     9781 tccgatcgta tatgaaatgc ctgaaggtgt ctccgttcaa actcctagcc aaacagagat
     9841 tgttttaacc ggctcggata aacaagttgt tggtcaagtt gctgctgaga ttcgtgcgtt
     9901 ccgtgctcct gagccttata aaggtaaagg tgttcgctat gtaggcgaag tagtggtaat
     9961 gaaagaagcc aagaaaaaat aattgaggtt cactaatgga taaacataca acccgactcc
    10021 gtcgtgcacg caaaacccgt gctcgtattg cggacttgaa aatggtaaga ttatgtgtgt
    10081 tccgaagcaa taatcatatt tatgctcaag taattagtgc tgaaggtgat aaagtattgg
    10141 ctcaagcctc tacattggaa gctgaggtgc gcggtagtct gaaatctgga agcaatgttg
    10201 aagcagctgc aatagttggt aaacgtatcg ctgagaaggc taaagcagca ggtgtagaaa
    10261 aggttgcttt tgatcgttca ggtttccaat atcacggtcg tgtgaaggct ttggctgaag
    10321 ctgctcgtga aaatggttta agcttctaaa tatttggaga ctttcagatg gcaaaacatg
    10381 aaattgaaga acgcggtgac ggtctgattg aaaagatggt cgcagttaac cgtgtaacca
    10441 aagtagttaa aggtggtcgc attatggctt tctctgcgct aactgttgtt ggtgatggag
    10501 atggtcgtat tggtatgggt aaaggtaagt caaaagaagt gcctgttgca gtccaaaagg
    10561 cgatggatca agcacgacgc tctatgatta aggtaccatt aaaaaatggt acgattcatc
    10621 atgaggttat tggtcgacat ggtgctacta aagtatttat gcagcctgct aaagagggta
    10681 gtggcgtaaa agccggtgga cctatgcgtt tggtttttga tgctatgggc attcataata
    10741 tctccgccaa agtgcacgga tctactaacc catataatat cgtacgtgca acattagatg
    10801 gtttgtctaa gttgcatact cctgctgata tcgcagccaa acgtggcttg acagtggaag
    10861 acattttggg agttaaccat ggctgaacaa aaaaagatta gggttacatt ggttaaaagc
    10921 ctgattggta caattgaatc tcatcgtgca tgtgcacgcg gtttaggttt gcgtcgtcgc
    10981 gagcatacgg tagaggtttt agatacccct gaaaaccgtg gtatgattaa taaaatcagc
    11041 tacttgttga aagtggagtc ttgatatgtt tttgaataca attcaacctg ctgttggtgc
    11101 tacgcatgct ggtcgtcgtg ttggacgcgg tattggtagt ggtcttggca aaacgggtgg
    11161 tcgtggtcat aaaggtcaaa agagccggtc tggtgggttt cataaggtgg gtttcgaagg
    11221 tggtcaaatg cccttgcaac gacgcctccc taaaagaggt tttaaatctt taacagcatc
    11281 agctaatgca cagcttcgtt taagtgaact ggaatcaatt gctgttaatg agattgatat
    11341 tttggtctta aagcaagcgg gtctgattgc atctacagtc tctaatgtta aagttattgc
    11401 ttttggtgaa atttctaagg cagttgcttt gaagggtatt aaagttacca aaggtgcgag
    11461 agctgctatc gaggctgttg gtggtaagat tgaaatgtaa ggtttaatat tgtggctaat
    11521 caacaaacgt catcaggttc atccaaattt ggagatctta agaaacgtct tttgtttcta
    11581 tttggagcat tgattgtttt tcgaattggt gcccatatac ccgtacctgg agttgatgct
    11641 gttgctttag ctaaattata cgaaagcgct ggaaacggca tcctgggaat attgaatatg
    11701 ttttccggtg ggtcgttaga gcgctttagt atatttgcaa taggaattat gccatatatt
    11761 tcagcttcta ttattgtaca gctcgcttct gaaattttgc catcattgaa ggctttaaaa
    11821 aaagaagggg aggctggtag aaaggtaatt acgaaatata ctaggtatgg tactgttttg
    11881 ttagcaattc ttcaaagtct aggtgttgca tctttcgtat ttcagcaagg aattgttgta
    11941 acaagttcat ttgagtttca tgtttccacg gtagtttctt tggtaacggg aactatgttt
    12001 cttatgtggc ttggggagca aattactgaa aggggtatcg ggaacggtat ttctttaatc
    12061 attacggcag gtattgcttc aggtattcct tcgggtattg caaagctggt tacactgacg
    12121 aaccaaggtt ctatgagcat gcttacggcg ttgtttattg tatttggtgc cttattatta
    12181 atttatttgg ttgtatactt tgaaagtgca cagcggaaga ttcctattca ttatgcaaaa
    12241 cgccagttta atggtagggc gggtagtcaa aatacgcata tgcctttcaa gttgaatatg
    12301 gctggtgtta ttcccccaat ttttgcttcc agtattattc tatttccatc tactctttta
    12361 ggttggtttg gttcggctga tacaaatagt gttttgcaca aaatagctgg attgttacaa
    12421 cacggtcaat tgctgtatat ggctttattt gcagcgacag ttattttctt ttgttatttt
    12481 tatacggctt tggtttttag ccctaaagaa atggcagaga atttaaaaaa gagtggtgct
    12541 tttgttcctg ggattagacc tggtgagcag acctctaggt atttagaaaa agttgtatta
    12601 cgtttgacat tgtttggagc tctttatatt acaactattt gtttaattcc agagttctta
    12661 actacggttt taaatgtacc tttttatttg ggtggcacgt ctttgttgat tctagttgtt
    12721 gtaacgatgg attttagtac acaaataaat tcgtataggc ttactcaaca gtatgataag
    12781 ttaatgactc gttcagaaat gaaatcattt tctcggaaat agaattatgg cgaaagaaga
    12841 tactatccaa atgcaaggtg aaattcttga aactttacct aatgcaacat ttaaagtaaa
    12901 acttgagaat gaccatattg tattgggtca tatttctggg aagatgcgga tgcattacat
    12961 tcgtatttct ccgggagata aggtcacagt agagctgaca ccttatgatc taactagggc
    13021 tcgaatcgtt ttcagagcaa gataaaccaa taaaaggaaa ataaaatgcg tgtacaacca
    13081 tctgttaaga aaatttgccg aaattgcaag attattcgtc gaaatcgtgt agttcgtgta
    13141 atttgtactg atctccgtca caaacagcgt caaggttaat ggaatatttc ttttaatgtg
    13201 attctgtgat atagtgacac actttgccct aaaaaggaaa aaatatggct cgtattgcag
    13261 gggtaaatat ccctaataac gcacacatcg taattggtct tcaggctatt tacggtattg
    13321 gtgctactcg tgctaaattg atttgtgagg ctgcaaatat tgcgcctgat actaaagcaa
    13381 aagatttgga cgagactcaa ttagatgctt tgcgtgacca agttgccaag tatgaagtag
    13441 aaggtgattt gcgtcgtgag gtaactatga gtatcaagcg attgatggac atgggctgct
    13501 atcgtggctt ccgtcatcgt cgcggcttac catgccgcgg tcaacgcact cgtacaaatg
    13561 cgcgtacccg caaaggtccg cgtaaagcga ttgctggtaa gaaataaatt ttaaggaatt
    13621 ttattaatgg ctaaagcaaa cacagcttca cgtgtacgta aaaaagtacg taaaaccgtg
    13681 agtgagggta ttgtgcacgt tcatgcatct ttcaacaata ccatcattac aatcactgac
    13741 cgtcaaggca atgcgttgtc ttgggctacc tctggcggcg ctggttttaa aggttctcgt
    13801 aaaagtacac catttgcagc acaagttgca gcagaagcag ctggtaaagt tgcccaagag
    13861 tatggcgtta aaaatttaga ggttcgtatt aaaggtccag gtccaggtcg cgaatcctct
    13921 gtacgtgctt tgaatgctct tggtttcaag attaccagca ttactgacgt taccccgttg
    13981 cctcataacg gttgccgtcc gcctaaaaaa cgtcgtattt aatattggag tgatttgaaa
    14041 catggcacgt tatattggcc ctaaatgtaa gttggcacgt cgcgaaggta cggatttgtt
    14101 tttgaagagt gcgcgccgct ctttggattc taaatgtaaa attgattccg ctcctggtca
    14161 gcatggtgca aaaaaaccgc gtttgtcaga ctatggtttg cagttgcgtg aaaaacaaaa
    14221 aatccgccgt atttatggcg tattagaacg tcagttccgt cgttatttcg cagaagctga
    14281 tcgtcgtaaa ggttctaccg gcgagttgct gttgcagttg ctggaatctc gtttggataa
    14341 tgtcgtttat cgtatgggtt tcggttctac ccgagctgaa gcaagacagc ttgtttctca
    14401 taaggcgata gttgtgaatg gacaagttgt caatattcct tctttccaag tgaaagctgg
    14461 tgatgttgtc tcagttcgtg aaaaagccaa aaaacaggta cgtattcaag aagcattggg
    14521 tttggcaact caaatcggct tgccgggttg ggtttctgta gatgcggata aacttgaggg
    14581 tgtgttcaaa aacatgccgg atcgctcgga attgaccggt gatattaatg aacagctggt
    14641 ggtagagttc tactctaaat aatgctagct cagtgaggga cagttaaatg cagaatagca
    14701 caaccgaatt tttgaaacct cgtcaaattg atgtaaatac tttttctgca actcgtgcaa
    14761 aagtatctat gcagccattt gaacgtggtt tcggtcatac cttaggtaat gctttgcgcc
    14821 gtatcttact gtcatccatg aatggttttg ctcctactga agtagctatt gccggtgtat
    14881 tacacgaata ttctactgtt gatggtattc aggaagatgt tgttgacatt ttgctgaata
    14941 ttaaaggtat tgtgtttaaa ctccatggtc gtagccaagt tcaacttgtg ttgaagaaat
    15001 caggttcagg tgtcgtatct gccggtgata ttgagttgcc gcatgatgta gaaattctga
    15061 atcctggtca tgtcatttgt catttggctg ataacggtca aattgagatg gaaattaaag
    15121 tagagcaagg tcgtggttat caatctgttt caggtcgtca ggtagttcgt gatgagaacc
    15181 gtcagattgg tgcaatccag ttggatgcga gcttttcgcc catcagccgt gttagctttg
    15241 aggttgaacc tgcacgtgta gagcagcgga cggatcttga taagttggtt ttggatatcg
    15301 aaaccgacgg ttctattgat cctgaggaag ctgtacgcag tgcggcacgt attttgattg
    15361 atcagatgtc tatttttgct gatttgcagg gtacgcctgt ggaggaggtt gaagaaaaag
    15421 cacctcctat cgaccctgtt cttttgcgtc cggtggatga tctggaattg acagtacgtt
    15481 cagctaattg tttgaaagct gaggatattt attatattgg cgatttgatt caacgcactg
    15541 aaaccgagct tcttaaaacg ccgaatttgg gacgtaaatc tttgaatgag attaaggaag
    15601 tattggcatc taaaggtttg acactgggtt ctaagttgga agcatggcca cctgtaggct
    15661 tggaaaagcc ttaatgaaga attaaaggat aattgatatg cgtcatcgta atggcaatcg
    15721 caaattaaac cgtaccagca gtcatcgtgc tgcaatgctg cgtaatatgg cgaattcatt
    15781 attgactcac gaaactattg taacaactct gcctaaggcc aaggaattgc gccgtgtagt
    15841 agagccgttg attacattgg gtaaaaagcc gtcattggca aaccgccgtt tggcatttga
    15901 ccgtactcgc gaccgtgatg ttgtagtaaa actgtttggc gatttgggtc ctcgttttac
    15961 tgctcgtaac ggtggttatg ttcgggtgtt gaaatacgga ttccgtaaag gtgataatgc
    16021 acctctggca ctggttgaat tggttgacaa accggctgct gagtaatttt agtcatataa
    16081 cgccatctgc cgaaaagcag gtggcgttat ttttgcaata tctgataggt aatagggtat
    16141 tggctatcat gtttaaaata ttaattgaat agctaaggtt tgcgcggtaa acttacatca
    16201 ttaaaaaatt ctatgatggt ttatataatg aatgctttcg atataaagtc gacaaagatg
    16261 gacgtattgt ctatatcttt gcatacgtca gacttgtttg atttggaaga tgtgctggtc
    16321 aaattgggca agaagtttca agagtctggt gttgttccat ttgtgctgga tgttcaagag
    16381 tttgattatc ccgagtcttt ggatcttgct gcattggttt cgttgttttc aaggcatggt
    16441 atgcaaattt tgggtctgaa gcattctaat gaacgttggg ctgctgcggc tatgaagtat
    16501 catttgctgt tttgtctgtc tcattcggaa aatgttaaag aactgggtca ggttgaggtg
    16561 cagaacacgg aggatggtca gaaagcaagg aaaacagtat tgattacatc ccctgtccgt
    16621 accggtcagc aggtttatgc cgaagatggc gatttgattg ttacgggggc ggtcagccag
    16681 ggggcggaat tgattgcgga tggcaatata catatttatg cgccgatgag ggggcgtgct
    16741 ttggccggtg ccaagggtga tacttctgcc cgcatattta tccactccat gcaggcagaa
    16801 ctggtttctg tggcgggtat ttaccgtaat tttgaacagg atttgccgaa ccatctgcac
    16861 aagcagccgg tacagatatt gttgcaggat aaccgattgg ttatcagtgc aattggctca
    16921 gagtaattgt ttgatatttt aaaaaggaaa tattgtggca aaaattattg tagtaacttc
    16981 aggtaagggc ggtgtcggta aaacgactac cagtgccagt attgcgacag gtttggcatt
    17041 acgcggatat aaaactgcgg taattgattt tgatgtgggt ttgcgtaacc tcgacctcat
    17101 tatgggttgc gagcgtcgtg tcgtttatga cctgatcaat gtcattcagg gggaggcgac
    17161 gctcaaccag gctttgatta aagataaaaa ttgtgaaaac ctgtttattt tgccggcttc
    17221 ccagactcgg gataaagacg ctttgacacg cgagggcgta gaaaaagtga tgcaggagct
    17281 gtccggcaag aaaatgggct ttgagtatat tatttgcgac tctcctgccg gtattgagca
    17341 gggtgcattg atggcgttgt attttgctga tgaagccatt gtaacgacca atcctgaggt
    17401 ttccagtgtg cgtgactccg acaggatttt gggaattttg caaagcaaat cccataaggc
    17461 agagcaaggc ggttcggtta aagaacatct gttgattacg cgttattctc ccgaacgtgt
    17521 ggcaaaaggc gaaatgctgt ctgtacagga tatttgcgat attctgcata ttcctttgct
    17581 gggtgtgatt cctgaatccc aaaacgtctt gcaggcatcc aattccggag aaccggtcat
    17641 ccatcaggac agcgtggcgg cttccgaggc atataaggac gttattgccc gtcttttggg
    17701 cgagaaccgt gaaatgcgtt tcttggaagc tgagaaaaaa agcttcttca aacgtctgtt
    17761 tggaggataa ggtatgtcat taatcgaatt tttattcggc agaaagcaga aaacggcaac
    17821 cgttgcccgc gaccgccttc aaatcatcat tgcccaagag cgcgcccaag aaggtcaggc
    17881 tccggattac ctgccgactt tacgtaaaga gttgatggaa gtcctgtcca aatatgtgaa
    17941 tgtttcatta gacaatatcc gtatttccca ggaaaaacag gatggtatgg atgtgcttga
    18001 gttgaacatt actttgccgg aacagaaaaa ggtataggac atgaccttaa ccgaattgcg
    18061 gtacatcgtc gcagtcgccc aagaacgtca tttcggcagg gcggcgcggc gttgttttgt
    18121 cagtcagccc actttgtcta ttgccattaa gaaattggaa gaagagcttg ccgtctcttt
    18181 gtttgaccgg agcagcaacg atattattac gaccgaggcg ggggaacgta tcgttgcaca
    18241 ggcgcgtaag gtgttggaag aggcagagct tatcaggcat ttggcaaatg aagaacaaaa
    18301 cgagctggag ggtgcgttca aactcgggct gatttttacg gttgcgccgt acctgctgcc
    18361 gaaactgatt gtctcgttgc gccgtactgc accgaaaatg cctttgatgt tggaagagaa
    18421 ttacacgcat actttgaccg agtcgctcaa acgcggggac gttgatgcga ttatcgttgc
    18481 cgaaccgttt caagagccgg gcattgttac cgaacccttg tatgacgaac cgtttttcgt
    18541 gattgtcccg aaagggcatt catttgagga actggatgcc gtttcgcccc ggatgctggg
    18601 tgaggagcag gttttgctgc tgacggaagg caactgtatg cgggatcagg tactctcaag
    18661 ctgttccgaa ttggcggcga aacaacgtat acaggggttg accaatacat tgcagggcag
    18721 ctcgattaat acaatccgcc atatggttgc cagcggtttg gcaatcagcg tgttgccggc
    18781 aaccgcactg accgaaaacg atcatatgct gttcagcatt attccgtttg agggtacgcc
    18841 gccaagccgg cgggtcgtat tggcgtaccg ccgcaatttt gtccgtccga aggcgttgtc
    18901 ggcgatgaag gcggcgatta tgcagtcgca gcttcacggg gtaagtttta tccgcgacta
    18961 ggcgcaggca ttgttttcaa aacgccattt ccctgagccg acaacacggt atgccaagat
    19021 attgccgtca tcatcgattt tgagtatagc atcgccacgg aaactgccgt cctgaagata
    19081 ttcgactttt gcatcactgt gaatgttttc atcagtgccg atgcaatgcc atgtatagtg
    19141 gattaacaaa aaccagtacg gcgttgcctc accttgccgt actatttgta ctgtctgcgg
    19201 cttcgtcgcc ttgtcctgat ttttgttaat ccactataaa agaggccgtc tgaaaaacat
    19261 ttttcagacg gccttgttga ttcaatcaaa tcagtctttc aacttcgaca actgagtttg
    19321 cacttttgcc attttgtctt ccaattccgc caaatcggct ttgtcttttt ccaccaaatg
    19381 cgccggggct ttttcggtgt agccgggttt ggagagtttg gcgttgagtt tgtccaaggc
    19441 tttttgcagc ttctcggctt ctttgctcaa gcgggcggtt tcggcagctt tgtcgatttc
    19501 gactttcaac atcaggcgcg cgccgttgca gacggcgacg ggcgcgtctt cgctttcggg
    19561 tagggcggcg acttgctgtg cttcggtcag gcgggtcatc atcggcaggt atttgaggta
    19621 gtccgccaag tcgtctgcgc tttcgacaaa cagcggggct ttcacgttgg gctggatgcc
    19681 catttcgccg cgcaggttgc ggactgcgcc gatcaaatcc tgcaacacgg tcatttgccc
    19741 gaatgccgtc tgaacaatct cgccgctgtc ggcttcgggg aagcgggcga gcatgatgct
    19801 gtcggcggtt ttcgcgtcgc acataggagc gacggtttgc cacagttctt cggtgatgaa
    19861 cgggataatc gggtgcagca ggcgcagggc ggcttcgagt acgcgcaata aggtatggcg
    19921 tgtggcgcgt tggcggctgg cgcagccggt ttgaagctgc actttggcaa gttctaaata
    19981 ccagtcgcaa tagtcgttcc acatgaagct gtacagggtt tccgctgcca aatcaaagcg
    20041 gtaggtttcg taggcttgcg ttacttgctc gatggtctga ttcagacggt ctacaatcca
    20101 catatcgggg aaggaatagc cgcgcggctc agtggcggtt gcgccgtagc cgcagtcttg
    20161 gttttcggtg ttcatcaaga cgaagttggt ggcgttccag attttgttgc agaagttgcg
    20221 gtagccttcg gcgcgtttga agtcgaagtt cacgctgcgc cccaagctgg cgtagctcgc
    20281 catggtgaaa cgcaatgcgt ccgcgcccat actcggaatg ccttcgggaa agagtttttt
    20341 ggtggcttct tccactttcg gcgcggtttc aggtttgcgc aggccggtgg tacgttttac
    20401 cagcagcttg tccaagccga tgccgtcaat caaatccaca ggatcgatga cgttgccttc
    20461 ggatttggac atttttttgc cttcgtggtc gcgcacgatg ccgtggatgt acacgtcttt
    20521 aaacggtact ttgccagtaa agtgggtggt catcataatc atgcgcgcca cccagaagaa
    20581 gatgatttcg tagccggtta ccaagacgtt ggacggcagg aaggctttga gttcgtcggt
    20641 ttcagacggc cagccgagcg tggaaaacgg cacgagcgcg gaggagaacc aagtgtccaa
    20701 tacgtcttct tcgcgggtca agcctgtttt gccggcttgt ttttcggctt cagcctgatt
    20761 gcgggcgacg tacacattgc ctgtttcgtc gtaccacgcg gggatttgat gcccccacca
    20821 cagttggcgc gaaatacacc agtcttggat gttgttcatc cattggttgt aggtattgac
    20881 ccagttttca gggataaagc gtaccgcgcc gctgtcgacg gcttttttgg ctttgtcagc
    20941 aaggcttaag cctttgaatt cgctgtcagg ctcgccgccg tttggcgtgg cggacatggc
    21001 gacaaaccat tggctggtca gcattggctc aatcactgag ccggttcggt cgcctttggg
    21061 tgtcatcagc gtgtgtgctt tgatttcgac caacaagcct tgttcctgca aatcggcaac
    21121 catttgtttg cgcgcggcaa agcggtctaa acctgcgtat ttttcaggca gggcaaagcc
    21181 ttgttgcgct tcgcctttga agttgaacac ttcggcgttt gccagcactt tggcttccaa
    21241 gtcgaacaca ttaatcaggc gcgtgtcgtg gcgtttgccg acttcgtagt cgttgaagtc
    21301 gtgcgcgggc gtgattttca cgcagccggt gccgaagtct ttttcgacgt attcgtcggc
    21361 aatcacggga atggtgcggc cggtcagcgg caggattaat tccttgccaa tcaaatgggt
    21421 gtaacgttcg tcttcaggat tgacggctac ggcaacgtcg cccagcagcg tttcgggacg
    21481 ggtggtcgcc acgatgacgg cttcggaggg attgtccgcc agcggatagc ggatgtgcca
    21541 catagagcct tgttcttcca cgctttccac ttccaaatcc gataccgccg tgccaagaac
    21601 gggatcccag ttcaccaggc gtttgccgcg ataaatcaag ccctgttcat acaggcgtac
    21661 gaacacttct gtcacggttt cggcgcgcac gtcgtccatc gtgaaatact cgcgcgtcca
    21721 gtcggcagag cagcccacgc ggcgcatctg ctgcgtaatc gtaccgccgg aaacttcttt
    21781 ccattcccac actttttcca agaatttttc gcggcccaag tcatgacggg acacgttttg
    21841 cgcggcaagc tgacgctcga ccacgatttg cgtggcgatg cccgcgtggt cggtgccggg
    21901 aatccagacg gtgttgcagc ctttcatgcg gtagtaacgg gtcagaccgt ccataatggt
    21961 ttggttgaag gcatgaccca tgtgcagcgt gccggttaca ttgggcggcg gcagttggat
    22021 ggaaaaagac ggtttggtca aatccatatc aggttggaaa tagccttggc tttcccagtt
    22081 ttgatagtgc ttggattcga tttcggctgg attgtatttg tctaacatga tggaactttg
    22141 tgaaattaag gttatttttg atgtgcggat tataacgcaa aaaggccgtc tgaatcattt
    22201 cagacggcct ttggcataca ggttttaaaa atggaacaat accaggctga cggcaatcac
    22261 cgccatgccc attgtcaggc cgtaaacggt ttcatggccg tctgaatagc gtttggcagc
    22321 cggcagcagc tcgtccaacg ccaaaaacac catcacaccg gctatcacgc cgaataccga
    22381 accaaacacg gcaggcgaca aaaacggctg caaaaccaaa tagcccaaag ccgcccccaa
    22441 cggctcggcc aagccggata gcagacacgc ccacaccgtt ttcttacggc tgcgggtggc
    22501 aaaataaacc ggcgcggcga tggaaatgcc ctccggaata ttatggatgg caatcgccaa
    22561 ggccaaaggc atcccgactg ctggattttc caatgtggca aaaaacgtcg ccaagccttc
    22621 ggggaaattg tgcgcagtaa tcgcaaacgc cgccatcatg ccgactcgcg cgatatggcg
    22681 gcgtttgctt tcttgaaacg acgggtcttg cgcgtctaaa gtttcatgcg ggttcggcac
    22741 cagacggtca atcagcgcaa tgccgcccat ccctgccaaa aatgccatgg tcgccgccgc
    22801 aaacgcgtgg tctttatcat aaatttcagc gaacgcctcg ctggacttac tgaaaatctc
    22861 cgtcagggaa acatacacca tcgcaccgcc ggcaaatgcc aaaccaaacg acaacacgcg
    22921 cggattgggc gttttggaaa acatcaccaa gccgctgcct aatacggtaa acaaaccggc
    22981 agccaacgta atggaaaagg caacggccaa attggacatc gaaaaatcgg gcatgagaaa
    23041 acctgcgcta aaagctggga caggttcaga ctaacacttt ttaatgtata tgataatagt
    23101 tattatttat tttattgatt ggatacacgg attttgaaac aaaaggccgt ctgaaaaatg
    23161 attttcagac ggcctttaaa tttgaaatgc cgctaaacct tagtgctttc cagcttaagc
    23221 ctgataacgc gacaggctca aatcgtcgct gcggatttcg gtgtctttgc cgctcacgat
    23281 atcggcggtt aattttgccg aacccagcga catggtccag cctaaagtac cgtggccggt
    23341 attcagaaac aggttgtcaa agcgggtgcg accgattaac ggcgtgctgt cgggcgtcat
    23401 cggcctgagg ccgctccaga acgatgcttg gctcaaatcg ccgccttccg ggaacaagtc
    23461 gttgacgacc aaagccaagg tttcgcggcg tttttcgggc agtttgattt cgtagcccga
    23521 caattccgcc ataccgccga cgcggattct gttgtcaaag cgcgtgatgg cgactttgta
    23581 gctttcatct aaaacggtgg acaccggcgc gccgtcggaa tttgtgaccg gcagggtcaa
    23641 ggaatagcct ttgacgggat aaatgggcag attgagatcc aactgcgcca aaaccgtcct
    23701 gctgaaacaa ccgagcgcgc agacaacggc atctgcttca aaccgccctg tttcggtttc
    23761 aacggttttg atgcgcagcc cgttgtggtc gatgcggctg atggtttggt tgaaatgaaa
    23821 ccgtacgccc ttttcctgac acaatttgta taggttttca gtgaagaggc ggcagtcgcc
    23881 ggttgcatcc gcaggcaggt gcaggccgcc ggcaattttg gcggtaacgc gtgccagcgc
    23941 aggctcgaat tctgcgcatt cttcgggctt cagacggcgg tacggcacgc cgtagcgttc
    24001 caaaacggca atgtcttgtt ctgcagcttc aacttcttta gcctgacgga aaatctgcaa
    24061 cgtccctttt ttgcgccctt caaaattcat gccggtttgc gcttcaaaac ggcggaacat
    24121 ttcacggctg tattcggaaa tcctgaccat gcgctcttta ttggtttgat agtgcgctgc
    24181 cgtgcagttt tgcagcatcc gccacagcca ttcgatttga tacaggctgc cgtcggggcg
    24241 aaacagcaaa ggcggatggc ttttaaacag ccatttcagt gctttggtcg gaataccggg
    24301 tgcagcccaa ggcgtggtat agccgtaaga aagctggcct gcattggcaa aactggtttc
    24361 catcgccaca cccttggcgc ggtcgatgac cgttacttca tgtccggcct ctgccagata
    24421 ccacgcggaa gacacgccgg caacacccgc acctaaaaca agcactttca tgtttctccc
    24481 tccgactttt tcaaaacaga cttaatatgc cgtgccgtct gaatattcgg attcagacgg
    24541 cctcggatat taatgcggca attcgccgtt tgtgattttt tgtttgaagt cgcgcgtttc
    24601 attgacgatg actttcgcca tcaataaaag tgcaatcagg ttgggcaatg ccatcaagcc
    24661 gttgaatgtg tccgaagcca gccacaccaa atcaaggctc aacacggtac ccagcataac
    24721 ggaagaaaca taacccacgc ggtacaaacc ggcaaatttc tcgccgaaaa catacaccgc
    24781 gcatttttcg ccgtaatagc accagcccaa aatggttgag taggcaaaga aaatcaggcc
    24841 gatggtaaca atccagccgc cgatgccggg cagcattttt tggaatgtga cggttgtcag
    24901 tgccgcgccg ctcacttcag gtttgacaaa ctcgccgccc gcgccgagca gtcccattac
    24961 caacacgatg ccggtaatcg agcaaacgac gatggtatcc aaaaacgtac cggtcataga
    25021 aaccaaggcc tgacggacgg gatggtcggt tttcgcggct gcggcggcaa taggcgcaga
    25081 acccataccc gcctcattgg agaacacgcc gcgcgccacg ccgtagcgga tgaccgtacc
    25141 gatagcgccg cccgccactg cctgcgcgct gaacgcatcg gagaaaatca gcttgacggc
    25201 aggcatcagt gcatcggaat taatcgcgat aatggaaaga ccgcccaaca cataaaacac
    25261 cgccatagca ggcacgatga aagaagcggc tttggcgatg cctttaatac cacctaaaac
    25321 gacaacggca gtcagaacgg tcaacgtaat gccggtatag gcaggttcga taccgaagct
    25381 ggtttgcacc gcctgtgcaa ccgagttgga ctgcaccgag ctgccgatac cgaaggaagc
    25441 gaatgtgccg aacagcgcaa atgcaacggc catccatttc cagtttttgc ccaagccttt
    25501 ttcgatgtaa tacatcgggc cgccggacat ttcgcctttg gaattgttga cgcggtattt
    25561 caccgccaac acgccttcgc cgtatttggt ggccatgccg aaaatggcgg tcatccacat
    25621 ccaaaatacc gcgcccgggc cgccggttac caccgcagtc gccacgccgg cgatgttacc
    25681 cgtaccgatg gtggcggaca gcgcggtcat caacgccgca aaatgggaaa tatcgccttc
    25741 gtggccttcg ccgccttcat acttctttga cggcacaaac gcctgtttca gcgcatagcc
    25801 caacatagtg aactgtaagc ctttcagcat aacggtcagc acaatgccgg tgccgaccag
    25861 cagcatcaac ataacagatc cccagaccca gccgctgacg gtttcgaaaa aggctttgag
    25921 attgtctaaa aacaattgca tggctttctc ctttgtctgt tttattttta aaacaccact
    25981 tttgtagtgt cctgtaattt cagcacagaa tattcaataa gacaatatgt tcttttgaaa
    26041 aatacttttg gttttttctc cgaaagcagg tcggttcaag ctgcggaaat tgtttgcaat
    26101 tctttaaaag cagcggcgga ggtcacaatg aaatgtccga atggggatgt ggcgggcggc
    26161 agaaatcatc aatgctgccg actgccatac ttctgaaatc tacaaaatga tgcatcgatc
    26221 aaacaatata ccgctttaaa aaaaccgatg ccgtctgaaa cgctttcggg gtttcagacg
    26281 gcatcaaaag ggtacggtca gcggatgatg ccgcgcgccg attgtgcgaa aaagtctcgg
    26341 aatacggcaa gctcggcttg ggtttcggcg cggcggagga tgtccgcctt ggcctcttca
    26401 aacggaatgc cgcgatggta gagggttttg tacacgtctt tgacggcgga aatctgctct
    26461 gcggtaaaac cgttgcggcg cataccttcg ctgttgaggc ccgccggttc ggcgcggtag
    26521 cccgatgcca taaagtaggg cggtacgtct ttgtgtacgc ctgcggcgaa ggcggtcatg
    26581 gcgtagtcgc cgatgcggca gaattggaaa accagcgtgt agccgcccaa aacgacgtag
    26641 tcgccgatgg taacgtgtcc ggcaagcgag gcgttgttgg cgaagatggt gtggttgccg
    26701 accacgcagt cgtgcgcgag gtggcagtac gccataatcc agttgtcgtc gccgatacgg
    26761 gtttcgccga tgccggttac cgtgcctaaa ttaaaggtgg tgaattcgcg gatggtgttg
    26821 ccgttgccga taatcagctt ggtcggctcg tcgcggtatt ttttgtcctg cgggatttcg
    26881 ccgaggctgg caaattggaa aatgcggttg ttttcgccga tgctggtgtg gccgttgatg
    26941 acggcgtgcg gaccgatttc ggtgttcgcg ccgatttgga cgttggggcc gataacagtg
    27001 tacgcgccga ctttgacgct tgagtcgagt tcggctttgg ggtcgatgac ggcggtcggg
    27061 tggatgaggg tcatgttttt aatactccgc tgcttgttaa gacaaataat agaaaatcac
    27121 agtttgtcga tttcatcgca gaccgcaatc acttgatcgc taaaatggct aaatactgaa
    27181 gcagtatcta catccatgta attaagcagt tcaaaaatag attggtggat atttctagcg
    27241 tagccggtat cttttattgc acctttaaaa caaatcggct cgtgatgtgc caaacgatta
    27301 cggaaattat taatattgga aagttctcga aaaatccatt tttggttatg ttgcacactt
    27361 ggtgtagatt tgggcttttt tggaaaaact ttcatcaata ctttccctgt agcatcaaat
    27421 tgggcatctt tccctcccgc aaacagatac cgccaaaaac cgaatcctag tcctgcgacc
    27481 aattggttgt ggtcaaactt tcctccgcta ttattttgta ttttagataa tgcaaccttg
    27541 attaattcag cagattcttt cgtaccattt cttaaaaagc aaccttgata tttcaatgca
    27601 ggattcgttt gtggctgtat gctgttatat aaccagtttc cgtctttaaa cgcctgctga
    27661 aggcaaatgt caattttgtt tcttaaaaca atctcaaata ttgaaacaac atgaaaaagt
    27721 tctcttgata aagataaatt caataaatac aattcagctg ctttgtcttt cccaacggca
    27781 tttttatacc gttccattct gaaatcagaa aaaatactac agcattcctt ataattcacg
    27841 ctaatacctg attccttatt gtatagtgga ttaacaaaaa tcaggacaag gcgacgaagc
    27901 cgcagacagt acaaatagta cggcaaggcg aggcaacgcc gtactggttt aaatttaatc
    27961 cactataaaa ttgacgtttc aaactatatt tgatacagtt aggtgaaact aatccccacc
    28021 gcgtccctgg gtttgaataa acctaaaccg gtgggttttc tttttattag gtcgtctgaa
    28081 actttcagac gacctttttc cgaacgctca aaccacgcgt ttggcgcaca taatcacggc
    28141 ttcgacggcg acttgcccgt ccactttggc aacggcgttg aatttgccga tgccgcgccg
    28201 gctggtcagc agctcgactt caaagacgag ttggtcgccg gggatgactt ggcgtttgaa
    28261 acgggcttcg tctatgccgg cgaagaagaa gaattcgttt tccttgcgcc cgccttcgct
    28321 gaggatggcg agtgtgccgc aggcttgcgc catggcttcg atgatgagta cgccgggcat
    28381 cacgggcagg tcgggaaagt ggccttggaa ctggggttcg tttatggtta cgtttttgat
    28441 ggcggttagg gttttcatcg gctcgaaggc ggtgatgcgg tcgagctgga gaaacgggta
    28501 gcggtggggg atgagttttt ggatgtcttt ggcttcgatg gggagttgaa cgtccatgtc
    28561 tgtcgtattc cttgaataaa gtcggtttgg atttattgcg tgtctttgcc gtctgaaagc
    28621 tgctgttcca gcgttttgag gcgtttgttc atttcgctta agcggtggat gtaaacggcg
    28681 ttgcgcgccc attctttatg ggtggacatc gggaagatgc cggcgaggtg tttgccgctt
    28741 tcggtaatgc tgtgggtgac ggacgtgccg ccgccgatgg tggttttgtc ggcgatttcg
    28801 atgtgtccga ccgtgccgac gccgccgccg atgatgcagt agctgcctat ggtgacgctg
    28861 cctgagatgc cggttttggc ggcgatgacg gtgtgcgagc cgattttgca gttgtgtccg
    28921 atttggactt ggttgtcgat tttggtgccg ttgccgacgg tggtgtcgct catcgcgccc
    28981 cggtcgatgt tggtgttcga gccgatttcc acgtcgtcgc ccagcgttac cgcgccagtt
    29041 tgcgggattt taaaccacga atcgccggcg aaggcgagtc cgaatccgtc cgcgccgatg
    29101 accgcgccgc tgtggatttc aacgcgtctg cccagtgtgc agccgtaata aacgacggcg
    29161 ttaggatgta aaacaacttc atcacctaaa cggcaatcgt gttggacgac ggcgtttgcc
    29221 aagatgcggc agccttcgcc gagcacggtg tttgcgccga tgtaggcgtt cgcgccgatt
    29281 tcgcagcttg cgggaacggt cgcgcccggt tcgacgacgg cggtcggatg gatgccgccg
    29341 cgcgctttga cgacgggtga aaataggcgc gcgactttgg cgaaatagag ataggggtcg
    29401 tcggcgacaa tcaggttgcg cccttcaaat ccgtctgccg ctttggcgga aacgatgacc
    29461 gcacccgcgc tgctgtcgtg gacttcggct ttgtatttcg ggttggcgag gaagctgatg
    29521 tgttccgcct gcgcgtctgc gagcgggcgc acggcggtaa cagaagtgtc ttcgccgcgc
    29581 cattcgccgc cgagccgcgc ggtgatttgg gacagggtgt aggtggccgg aatcatggtt
    29641 ttcctgttcg gtatgccgtc tgaaagggtc agcgggcgtt catttcttta atgacgctgt
    29701 cggtaacgtc gtattgggtg ttgacgtaaa tcacgtcctg caaaatgaca tcgtaacctt
    29761 cctgtttggc gattttgacg atgacgcggt tggcgttttg ctggagggag gcaaactctt
    29821 cgttgcggcg gaggttgtag tcttcttcaa actgcgcctg ttttttgcgg aacgctgcga
    29881 ccagcccgca ccatttttct tcggcttgcg ccttttttgc gtctttgagt ttgccttcgg
    29941 caagctgcct ttccaaatcc agaccttcgc gttgcagttt ttgcaattcg tcctggcggg
    30001 cggaaaattc gctgtccagc gttttttgaa tcttgcgcgc ctgcttggat tcgaggtaga
    30061 tgcgctcggt gttgataaag ccgatttttt ggaaggtgtc ggcgtgcgcg cctgcggtgc
    30121 agcacaaacc gatcagagcc gcggcaaacg cgcgggtcaa acgggtcatg gtaaaactcc
    30181 ttcgaatgtt gccgcgaaat gccgtctgaa gggcttcaga cggcatttgc gggattagaa
    30241 cgtcgtgccg agttggaatt ggaagcgttg gatttcgtct tccggttttt tcttcagcgg
    30301 gtaggcgtag ctgaatttca tcgggcctaa aggcgagagc caggtaaccg cgccgccggc
    30361 ggaatagcgc aattcgttgg taaaggtgga tttatgggta ttgccggcgc cgtaaatgtt
    30421 ttgaaccctg ccgccggtcg cggaactgct gttgtcgtcg taggttttgc cgtcccacac
    30481 gctgcctgcg tcggcaaaca ggctcaggcg gacggtgcgc gcgtctttcg cgccgggcat
    30541 cgggaagagc agctcggcgg agacgttggc ttttttgttg ccgccgtagc tgattttttc
    30601 gccgtattcg tcatacactt tcggaccgag cgtgccgctt tcgtatccgc gcaccgaacc
    30661 caggccgccg ccgtagaagt tttcaaagaa ggggatttct ttggttctgc cgtagccgcc
    30721 cgcaatgccg acttcgccgc cgagcatcag cgtgaaggtt ttgcttaagg ggaagaacca
    30781 ggtttggttg tgggtggcgg agtagtattg cagtttgctg ccgggcaggg cgatttcggc
    30841 gttcacgccc gtcaggtagc cgcgcgtcgg ccataacgcg ctgtcggttt tgttgcgccc
    30901 ccagccgacg gtacctttgt acagccagcc tttgaagctg ccgtctgtgc cgtcggtttt
    30961 gccgtatttc ttgataaagt cggcatagtg tttgggcgct ttgttgtagg tgttgacggt
    31021 caggtgttct gccaccaaac cgaaattcac gcggtcgtat tcggtaacag gcacgctcat
    31081 gcggatgcct gcgcctgccg tggtggtttt atattgtttg atgctggtcg atgctttgcg
    31141 cgggtcgaag gcttttccgt aaacatcgta gcccaggctg accccgtctg ccgtgaagta
    31201 cgggtcggta aacgacagcg agccgttgag cgtggttttg cttcgtgagg cgcgcagggc
    31261 ggccgacttg cccgtaccga acaggttgtc ttgggaaacg cctgcggaca tgaccaggcc
    31321 ggtatcctgt acccagcccg cgctcaagtc gagcgagccg gtggaacgtt cggtcaggct
    31381 catgttcaaa tcgactttgt cgggtgtgcc ggcaagcggg acggcatcaa actgtacgtt
    31441 gtcgaagtag cccaaaagct cgacgcgctc tttggagcgt tgcagcttgg aggtgtcgta
    31501 aggcgcggat tccatttggc gcaattcgcg gcgcacgact tcgtcgcggg ttttgttgtt
    31561 gccggtgatg tggatttcgt tgacgtagat tttccggccc ggttcgatgt gcaggacgaa
    31621 atcgacggtt ttggtttcgg cgtttggcag cggctgtacg ctgatttcgc tgtatgcgta
    31681 gcctgccgag cccatgcggt tctgaatctc acccaaaacg gcggtcatct gctggcgttc
    31741 gtaccatttg ccgggcttca tggtcagcag tttttccagt tcggctttgg ggacttcgtt
    31801 ggtgtcgcct tcgatggaga ctttgcccca acggaaacgt ccgccttcgt ggacggtgat
    31861 tttgatggtc tgcttggttt tgtcttcgtt ggtttggatg tcggtatcga ggatgcggaa
    31921 atcgaagtag ccgttgttct ggtagaagtc ggttactttt tccatgtctt gggcaaattt
    31981 ctgctcgttg aattggttgc ttcgtgtcag ccatgtccaa atgccgcctt cggtcagcga
    32041 catctgccgc atcagtttgc ggtcggaata gacttggttg ccttcaaatt cgatgtcggt
    32101 gattttggcg gatttgccct cgtcaatcgt gatgtcgatg tcgacgcggt tgcgggcgag
    32161 tttggttact ttgggcgtga tttggatatt gagtttgccg cgcccgaggt attcttcttt
    32221 caggccggcg actgcctgat tgagtgtcgc ctgattaaag tattgcgact gcgccagccc
    32281 gaacgattcg aggtttttct taatggcgtc gttttgcagc atttttgcgc cggtgatgtt
    32341 gagcgagccg atggtggggc gttcgatgac ggtcagcagg agctgcccgt ccgcagtctc
    32401 gacgcgtacg tcgtcaaaga aaccggtggc gtacaggctt ttgatgatgg cactgccgtg
    32461 tgtgtcgttg taggtgtcgc cgactttgac gggcaggtag ttgaatacgg tactcggctc
    32521 ggtacgctgc aagccttcga cgcggatgtc ttggatggtg aagtcggcaa gtgccaaagg
    32581 cgatatgccc aagaccatca gtgcggaagc aatctgtttc agtttcattg tcagttcctt
    32641 gtggtgcgga atgcggtttc agacggcatt ccgaaacgta aaatctaacc gagcagccgg
    32701 gtaacgtcgt tgaagaaggc gaccgccatc atcagcatca tgagggcaag cccgaagcgc
    32761 aaaccgatgt tttggacgcg ttcgcccaaa ggtttgccgc gtatccattc ggcagtataa
    32821 aacacgaggt ggccgccgtc caaaacgggg acgggcagta ggttcagcac gccgaggctg
    32881 atgctgacca gtgccaaaaa ttccaaataa ctttgcaagc cgagttcggc ggactgtccg
    32941 gcaatgtcgg caatggtcag cggcccggaa atatggctga cggaggcgtt gccgctgatt
    33001 agtttgccga aaaatttgag ggttgtccac gagtgggaaa cggttttttc ccagcccatg
    33061 ccgaatgcgc ggataacaga cggacggtag ctgcggcgga tttgcgcgtc ccacgccctg
    33121 tccggctgcg gacggaggcc gacgcgcccg atcagggtgt ggtcggactg ttcgacagta
    33181 tcggggcgga tgtcggcggt atgggtttgt ccggcgcgtt cgtagttcag ggtgattttt
    33241 ttgccggggc tttggcgggt caggtttgcc cattcttgcc atgaggcgat gggtttgccg
    33301 tcggcggcag tcagcctgtc gcccggtttc aggcctgctt tttcggcggg gctgcctttt
    33361 tccacgccgc cggcaacggt tgtgatttta aagggcatca gtccgatgta gccttggttt
    33421 tttgcgattt taccggcttc cggcgtgcct gcggcatcga tggtgcggac ggtttgcgcg
    33481 cccgatgccg tctgaacgcc gacggcgact ttgccggctt cgaggttgag gacgatttcg
    33541 gtttgcgcgc tgccccaatc tgcaacgggt gtgccgttga cggattgtat tttgtcgccg
    33601 ctttggaagc cggcgcgggc ggcaatggtg tcaggttcga ctgtgccgac gtaggggcgc
    33661 agttcggtta cgccgaagga aaagctcagt ccgtacagca aaaccgccag tgcgaggttg
    33721 gtcagtgggc cggcggcgac gatggcgatg cgcttggcgg ggtgttgttt gtcaaaagcg
    33781 tagggtaaat cggcttctga tacttcgcct tcgcgcgtgt cgaccatttt gacgtaaccg
    33841 cccaacggaa tcggggcgag gcaccattcg gtgtcgccgc gctttcgggt gaaaaacggt
    33901 ttgccgaagc cgacggaaaa gcgtacgact ttgacaccgc acaatctggc aacgatgtag
    33961 tgtccgaact cgtgcaggct gaccaaaatc aggatggcga agataaaagc tagaagggtg
    34021 tgcaaatggt tttcctttga taacggtgtt cggacggcat cagcgcagtg tgccgataaa
    34081 tgcccgcgct tgtgcgcgtg tccgggcatc ttgcgccaag agccccccta tgtcacctat
    34141 gccgtctgaa aagtcttgtg aaagacaatg ggcgacggtt ttggcaatgt cggtaaactt
    34201 aatctgtccg tccaaaaagg cggcgacggc ggcttcgttg gcggcgttca atacgcaggg
    34261 cgcgaccccg cccgcgttca tggcttcata ggcgagcttc aggcagggga agcggtcaaa
    34321 gtcgggcttt tggaaggtca gcgcggacaa tgcgtcgaaa tcaaggtcgc cgacacccga
    34381 atcgatgcgc tcgggcaagc ccaaacaata ggcgatgggc gttcgcatat cgggattgcc
    34441 cagttgtgcc agcacggagc cgtcgcggta gcgcaccatg ctgtgtatca cggattgcgg
    34501 atggatgacg acttcgagtt tgtcgggcgg acagttgaac agccaatgcg cttcaatcag
    34561 ctccaaacct ttgttcatca tggtggcgga atcgacggag attttgcgcc ccatacgcca
    34621 attggggtgt ttgaccgctt gggcgggcgt aatgcggtcg aacgtgccta aatcggtcgt
    34681 taaaaacggg ccgccggaag cggtcaggat aatcgaacgg atgccgtgtt cgttcagacg
    34741 gccggcgtaa tcgcgcggca aaacttggaa aacggcgttg tgttcgctgt cgacgggcag
    34801 cactgccgcg ccgtttgcac gggcggtttc cataaacaac gcgccggaaa ccaccagcgt
    34861 ctctttgttc gccagataaa tggttttgcc tttttgcgcc gctgcgagcg cggaaggcag
    34921 ccccaccgcc ccgacgatgg cgcacatgac accgctgact tcgtcggcag aggcaacgtc
    34981 aaccaatgcc tgcgcgccgt gtaaaacctg cgtcgccgtg ccgtcgcgtt tcaacagggc
    35041 ttcaagccgg gcggcgtatt cggcatcggc aacgacggca tattcgggtt ggaacgtttg
    35101 acattgagcc gccagtttct cgacctgctt atgccctgcc agcgcgaata cgcggaattt
    35161 ttcggggtgg cgggagacaa cgtccagcgt gctttcgcct atgctgccgg tactgcctaa
    35221 tatggtcagg acttgtggtg tcataatggg gataacttta taccggatgc cgtctgaagc
    35281 gttttcagac ggcatagaat caatttaaaa ccgacatcat cgccgcatag acgctgataa
    35341 cggcaatcag gctgtcggta cggtcgaaca cgccgccgtg tccgggcagc agcttgctgc
    35401 tgtctttgat gcctgccgcg cgcttgagcc agctttccaa aaggtcgccg catacgctga
    35461 caacggtcaa caccaaaccg attaacacgg tatcgaacca gcctgtatcg aatgccagcc
    35521 agccggcact tcgtacggcg gtcatgtaca cggccacgca aaccgcgccg ccgattgcgc
    35581 cttcccagct tttgccgggg ctgattgccg gtgcgatttt gtgtttgccg aacgccttgc
    35641 cgctgaaata cgcgcaaata tcggcaaccc acaccaaacc catcacggcg agcagcggca
    35701 gggcatcatc gggatgcggg cgcagggata cgagcgcgaa ccaaaacggc atgaccagaa
    35761 gccagccgac ggcataaacc tgccaaccgc cgttgagcct ccatttgaat ctcaaccata
    35821 aaggcataac ggcgagccaa aatgccaaaa caacatacca aaccaaatta ggcagcatcc
    35881 agccgcccgc ataggcaacc acgccgaaaa ccaaggttgc ggcgaggtaa tggttggttt
    35941 taattttgca caaaccgccc atacgggcat attcccacaa ggcaatcagg gcaatcagtc
    36001 cgcaaaatgc agcccacaac cattgcggcg cgtaaaacag catgcccagc atcagcggca
    36061 gcagccacat ggcggtcatt acccgttgtt tcagcatatt cagttccttt gctgttcgat
    36121 aggcagttgc tcggaggtgc gtccgaaccg ccgttcgcgt ttttggaacg aagcgacggc
    36181 atcgtccaaa gccttgccgt caaaatcggg ccacaaaata tcggtgaaat acagttcggc
    36241 ataagccatc tgccagagca ggaaattact gatgcgcgtt tcgccgccgg tgcggatgaa
    36301 caaatccggc tcgggcgcgt ctcccagcat caggtatttt gccagcgtgt cttccgtaat
    36361 ctcggatacg ccttcggcaa tcagtttgtt tgccgcctgc aaaatatccc aacgtccgcc
    36421 gtaatcggcg gcaatgctca gggtcaggcc ggtattgttt gccgtcaagg cttccgcctc
    36481 ttcgatgcct tgcagaatct gccgattaaa gcgttcgcgg ctgcccagta ttttcaggcg
    36541 catattgttt tcgtgcaggc ggcgtacctg tttttgcaaa gcctgtaaaa acagccccat
    36601 caggaacgaa acttcgtctt cggggcggcg ccagttttcg gtcgaaaagg caaatacggt
    36661 cagatattgc acgcccaatt tggcgcaatg cctcaccata ttttccaatg cgtccaaacc
    36721 gcgtttgtgt cccattatgc gcgggaggaa acgttttttc gcccaacggc cgttgccgtc
    36781 cataatcacg gcgatatgct tgggaatggc ggtgtgttcc aaaacggcct gcgtgctgct
    36841 tttcatgtct gcctttcgcg gttcggcatt caaatgccgt ctgaacgccg aaccgtgcag
    36901 gttaaattgc catcaaatct tcttctttgg cagtcaggag tttgtcggct tcggtaatgt
    36961 atttgtcggt cagtttttga accgcttctt cgccgcgacg tgcctcgtct tcggaaattt
    37021 ctttgtcttt gaggagtttt ttgatgtggt cgttggcatc gcggcgcacg ttgcggatgg
    37081 agacgcggcc ttcttccgct tcgccgcgta cgactttaat caggtctttg cggcgttcct
    37141 cggtcagcat gggcatcggc acgcggatca ggtcgccgac agctgccggg ttcagtccca
    37201 agtttgaatc gcggatggct ttctcgactt tggccgccat attgccctca aacggtttca
    37261 cgccgatggt gcgcgcatcc agaagcgtta cgttggcaac ttggctgacg gggaccatgc
    37321 tgccccagta ttcgacttcc acttggtcga gcaggccggt atgcgcgcgg ccggtacgca
    37381 ctttcgccag attttctttc agtacttcga ccgaacgctg catcttgcct tcggctgttt
    37441 tttgaatatc gttgatcata ttgttctttc ggtaagataa ggtaagcggg cagccgtctg
    37501 aaacgcgctt caaacctttc agacggcatg aaaactgtta actgcgaata gtaccgttat
    37561 tcgggcatga cgacaaggca ggcggattgg ggatgccgtc tgaagcggca ggcgtttcag
    37621 acggcatcgt gtccgaccgt cagccgtgtt cccgtgtttc aagcaggctt tggcgcaggt
    37681 gttggcgttc gtgggcatcc agccatttgc ggcgggtgcg ttgcagcagg atgacgaggg
    37741 cggaaatttc ctgacgcata ttggtgctga gccagaggaa gccctgccat tggtagtgga
    37801 ggtgttcggc gagggcttcc agttcggggt tgatggcggt gtcgatgcgg atgcggcggg
    37861 cgtgtctgcc gttgataagg gcgacggttt gttgcaggtc ggtttggagc agtgtgaagt
    37921 ggcggtcaag cagccggatt tcgctgccgt tgagtttggg agattgcagc ttggcggcag
    37981 tggtcaggag cagctcggtg gtgttgacaa ttttacggtg ggcgtgctgc atggcttcca
    38041 tcatggcggg gctgatgcgg ctttcgcccg atgtggcggc gaggtggctg cggcttttga
    38101 ccatgcgtgc gttgatttgg cgcattttaa ccatattctg ctccaaacgt tcgcgcgtca
    38161 tacgcctgcc gttgctgatt tcggcaatca ttttgctgca gtcggtcagg ttgtcggcaa
    38221 gcatgaaacg ccacatcagt gtggatttca gcggcagcag tttggcggcg gcgatggcga
    38281 tggccgcgcc gatgaggacg ttcatcgcgc gcatcaggcc gctgtcgaac cattcgctgc
    38341 cgttgtcgcc gatgagcatg cacatcgtca gccccgccag catagggacg tagccgtttt
    38401 tgccgaccgc cgcccagccg gccagtgcgc ttgccgtgcc gacggtgagg tagaagagga
    38461 ggttgccgtg gaaataatgc tggttcagcc ataaaacgcc caaacccgcg cccagcccga
    38521 tgaccgtgcc gagcatacgt tccaccgcct tggagtaaat cgcgccttga aactggagca
    38581 tgccgaggac gacgaagacg gtcatcccta tccactcgcc gtgttggagg tggagcagcc
    38641 gggcggaggc ggtggcgaac agaacggccc cgccgagccg gacggcgtgg atgaggcggc
    38701 ggtagcggta gcgttcgtag gagttgagcc agcggctgac gaggcggttg cgttgcgagg
    38761 tgttcatatc ggttgccgtc tgaagcggaa atgtgaaaaa gcacaggctt cccgagggag
    38821 ggtctgtgct tggtattggt gccggagaag ggaatcgaac ccccgacctt cgcgttacga
    38881 atgcgctgct ctaccgactg agctacaccg gcgtttttcg ttatgatata tatgaacggt
    38941 tgtttgtgca acttttctgg cgggcggcaa ggcagtgcgc ggtatagtg
//
LOCUS       DAWFLT010000011        36766 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWFLT010000011 DAWFLT010000000
VERSION     DAWFLT010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 36766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36766
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.011"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(177..1619)
                     /gene="aldA"
                     /locus_tag="WGR74_000536"
     CDS             complement(177..1619)
                     /gene="aldA"
                     /locus_tag="WGR74_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ0905020.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRGRADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1654..1854)
                     /locus_tag="WGR74_000537"
     CDS             complement(1654..1854)
                     /locus_tag="WGR74_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905021.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTDANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTAIYLGIN"
     gene            complement(1890..2252)
                     /locus_tag="WGR74_000538"
     CDS             complement(1890..2252)
                     /locus_tag="WGR74_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905022.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLVN
                     YSYLDNCLVTLTADRISV"
     gene            complement(2461..3378)
                     /locus_tag="WGR74_000539"
     CDS             complement(2461..3378)
                     /locus_tag="WGR74_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0905023.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWDLPPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFVGGA
                     HQVNRLTDALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSVGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3550..4350
                     /locus_tag="WGR74_000540"
     CDS             3550..4350
                     /locus_tag="WGR74_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0905024.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLSGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTQLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4398..5174
                     /gene="mlaE"
                     /locus_tag="WGR74_000541"
     CDS             4398..5174
                     /gene="mlaE"
                     /locus_tag="WGR74_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ0905025.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAIDFVLTAWMFTD"
     gene            5226..5720
                     /gene="mlaD"
                     /locus_tag="WGR74_000542"
     CDS             5226..5720
                     /gene="mlaD"
                     /locus_tag="WGR74_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ0905026.1"
                     /translation="MKKNILEFWVGLFVLLGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5757..6347
                     /locus_tag="WGR74_000543"
     CDS             5757..6347
                     /locus_tag="WGR74_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ0905027.1"
                     /translation="MKKSSFISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTTRQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            6404..6682
                     /locus_tag="WGR74_000544"
     CDS             6404..6682
                     /locus_tag="WGR74_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ0905028.1"
                     /translation="MHTELNNGTLHISGDVTVKTLTGDAFERFRQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6679..7506
                     /locus_tag="WGR74_000545"
     CDS             6679..7506
                     /locus_tag="WGR74_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ0905029.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7631..7993
                     /locus_tag="WGR74_000546"
     CDS             7631..7993
                     /locus_tag="WGR74_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ0905030.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIETRFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7990..8376
                     /locus_tag="WGR74_000547"
     CDS             7990..8376
                     /locus_tag="WGR74_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0905031.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            8373..8861
                     /locus_tag="WGR74_000548"
     CDS             8373..8861
                     /locus_tag="WGR74_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0905032.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8943..9110)
                     /locus_tag="WGR74_000549"
     CDS             complement(8943..9110)
                     /locus_tag="WGR74_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ0905033.1"
                     /translation="MEDDVWPGHRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(9077..9457)
                     /locus_tag="WGR74_000550"
     CDS             complement(9077..9457)
                     /locus_tag="WGR74_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ0905034.1"
                     /translation="MGSKFFFLLLRFAGSGLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMEPECLFYSNNHKFDRS
                     KNALRATRKSVRLRWRTMSGRGTG"
     gene            9467..9593
                     /locus_tag="WGR74_000551"
                     /pseudo
     CDS             9467..9593
                     /locus_tag="WGR74_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            9695..9910
                     /gene="rpmE"
                     /locus_tag="WGR74_000552"
     CDS             9695..9910
                     /gene="rpmE"
                     /locus_tag="WGR74_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEZ0905035.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
     gene            10225..10839
                     /locus_tag="WGR74_000553"
     CDS             10225..10839
                     /locus_tag="WGR74_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ0905036.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGIYILVENNSFDMLWTVLG"
     gene            11000..12676
                     /locus_tag="WGR74_000554"
     CDS             11000..12676
                     /locus_tag="WGR74_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ0905037.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADSYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPTVHFLNPAAAAFAAAGLV
                     LHGYSLACRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAANPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADASQGWQTVWQGARPRNKDSKFALIRKIGENILKTTD"
     gene            12778..13383
                     /locus_tag="WGR74_000555"
     CDS             12778..13383
                     /locus_tag="WGR74_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ0905038.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAQLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            13454..13846
                     /locus_tag="WGR74_000556"
     CDS             13454..13846
                     /locus_tag="WGR74_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ0905039.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFSGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(13875..14024)
                     /locus_tag="WGR74_000557"
     CDS             complement(13875..14024)
                     /locus_tag="WGR74_000557"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905040.1"
                     /translation="MPSEARIAGSDGIFNVPISNRQGQHGFDTPKRVLPINGFRLRPI
                     GQNAV"
     gene            complement(14045..14491)
                     /locus_tag="WGR74_000558"
     CDS             complement(14045..14491)
                     /locus_tag="WGR74_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ0905041.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(14623..14835)
                     /locus_tag="WGR74_000559"
     CDS             complement(14623..14835)
                     /locus_tag="WGR74_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ0905042.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(15124..16974)
                     /locus_tag="WGR74_000560"
     CDS             complement(15124..16974)
                     /locus_tag="WGR74_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0905043.1"
                     /translation="MYLPSMKHSLPLLATLVLAACSSTNTLPAGKTPADNIEAADLSA
                     SVPTRPVEPEGKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKS
                     LGARRQWTLFAQEYAKLKPEGRAQEVVCYADLKHSDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDSNNAWRRVRGLLANSQTTDARNLAAALGSPFDGSTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSPEQRSFAWGVLGHYQSQSLNVPAALDYYGKVSDRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSHAALGNLQQAAKL
                     YEQAAATGRNFYAVLAGEELGRKIDTRNNVPDADTNSVRRMAEDGAVKRALVLFQNSQ
                     SAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQFYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMTNAAYYAALFGAPHIPLKQRMGIVPAR"
     gene            17504..18241
                     /locus_tag="WGR74_000561"
                     /pseudo
     CDS             17504..18241
                     /locus_tag="WGR74_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
     gene            18243..18929
                     /locus_tag="WGR74_000562"
     CDS             18243..18929
                     /locus_tag="WGR74_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0905044.1"
                     /translation="MADLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRTIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVCKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            19089..19952
                     /locus_tag="WGR74_000563"
     CDS             19089..19952
                     /locus_tag="WGR74_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905045.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAEKKE
                     IVFGTTVGDFGDMVKEHIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(20014..20583)
                     /locus_tag="WGR74_000564"
     CDS             complement(20014..20583)
                     /locus_tag="WGR74_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ0905046.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            20742..21602
                     /locus_tag="WGR74_000565"
     CDS             20742..21602
                     /locus_tag="WGR74_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ0905047.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGKIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            21738..22076
                     /locus_tag="WGR74_000566"
     CDS             21738..22076
                     /locus_tag="WGR74_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905048.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRNNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            22444..22797
                     /locus_tag="WGR74_000567"
     CDS             22444..22797
                     /locus_tag="WGR74_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ0905049.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAVWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            22787..23653
                     /gene="atpB"
                     /locus_tag="WGR74_000568"
     CDS             22787..23653
                     /gene="atpB"
                     /locus_tag="WGR74_000568"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ0905050.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            23710..23946
                     /gene="atpE"
                     /locus_tag="WGR74_000569"
     CDS             23710..23946
                     /gene="atpE"
                     /locus_tag="WGR74_000569"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ0905051.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            24017..24487
                     /locus_tag="WGR74_000570"
     CDS             24017..24487
                     /locus_tag="WGR74_000570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ0905052.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            24492..25025
                     /locus_tag="WGR74_000571"
     CDS             24492..25025
                     /locus_tag="WGR74_000571"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ0905053.1"
                     /translation="MAEFATIARPYAKALFGLAQEKSQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            25036..26583
                     /locus_tag="WGR74_000572"
     CDS             25036..26583
                     /locus_tag="WGR74_000572"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ0905054.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIYGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            26608..27483
                     /gene="atpG"
                     /locus_tag="WGR74_000573"
     CDS             26608..27483
                     /gene="atpG"
                     /locus_tag="WGR74_000573"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ0905055.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            27521..28918
                     /gene="atpD"
                     /locus_tag="WGR74_000574"
     CDS             27521..28918
                     /gene="atpD"
                     /locus_tag="WGR74_000574"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ0905056.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            28929..29351
                     /locus_tag="WGR74_000575"
     CDS             28929..29351
                     /locus_tag="WGR74_000575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ0905057.1"
                     /translation="MSIMQVEVVSSEQNIYSGEASFVVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            29674..30579
                     /gene="glyQ"
                     /locus_tag="WGR74_000576"
     CDS             29674..30579
                     /gene="glyQ"
                     /locus_tag="WGR74_000576"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0905058.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAVEGASLALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            30656..31066
                     /locus_tag="WGR74_000577"
     CDS             30656..31066
                     /locus_tag="WGR74_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEZ0905059.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQTLIFGVQGSLHQAA
                     DDAEAFGRFMDELIAYLEANGLEMAGGGDCGDFSCLFQKSGARSLGETEKKAIISWLA
                     GREDVATLYATPLVDGFYGDDGWENYSETYKQAD"
     gene            31087..33150
                     /locus_tag="WGR74_000578"
     CDS             31087..33150
                     /locus_tag="WGR74_000578"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ0905060.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGGLLEDIINQVVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARSLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLSSVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAVEQHYQPRFAGDKLPESKIA
                     TAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGVVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            33167..34256
                     /locus_tag="WGR74_000579"
                     /pseudo
     CDS             33167..34256
                     /locus_tag="WGR74_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259429.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            34256..35095
                     /locus_tag="WGR74_000580"
     CDS             34256..35095
                     /locus_tag="WGR74_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0905061.1"
                     /translation="MQNHVISLASAAERRAHITDTFGVRGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLQERFDPDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISR
                     KAMRFFLDRFAALPPEGLHPVDWMMFSDFFDREGMPVCQLDPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQRRDSPANTFKHRLIRALTKISREREKRRQRREQLIGKIIV
                     PFQ"
     gene            35107..35202
                     /locus_tag="WGR74_000581"
     CDS             35107..35202
                     /locus_tag="WGR74_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HEZ0905062.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILHRLLKNR"
     gene            35244..36050
                     /locus_tag="WGR74_000582"
     CDS             35244..36050
                     /locus_tag="WGR74_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0905063.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHDIPFQFFDALMPSEALEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLQERFDKDSAFIVRLETMFMHVLTSPSGVADYGGRAFPLLESEHFGMAGYIISR
                     KAMRFFLDRFAVLPSERLKAVDWMLFSSFLDKGGMTVCQLTPALCVQSETLPSQLKNG
                     RQESYRNRRSPKVLLKRALGKIGREIERARERKRQKKLEKHLGRHVVPFE"
BASE COUNT         8687 a         9784 c         9484 g         8811 t
ORIGIN      
        1 agtgaatcgg ttccgtacta tctgtactgt ctgcggcttc gtttaaattt aatccgctat
       61 acaaagacaa aaacatcgac ctcggtcatg actgattgcc gatgaagcaa taaaaatgcc
      121 gtctgaacct gaaaacgggt tcagacggca ttttctatcg gggttttaaa gcggcattaa
      181 atgtcggttt ccaaataaac gacttgggtt tgcagatatt cttccaaacc gtgtttgccg
      241 tccgcgccgc cgataccgga ttttttccaa ccggcgtgga aaccctgcat cgcttcaaag
      301 ttttcgcggt tgatgtaggt ttcgccgaat tgcaggcggc gggtaacgta gaaggcttcg
      361 tttaaattag tcgtataaac agaactggtc agaccaaact cgcaatcgtt agccagtgcg
      421 atgacctggt cgagcgtgtc gaaagcggaa acgggcagca cggggccgaa ggtttcttct
      481 ttcataatgt ccatactgtt gtcggtgtcg gtcagcaggg tcggctcgaa gaaataaccg
      541 cgtccttcgg cgcgtttgcc gccgcaaacc aatttcgcac cttgtttgac tgcccgttcc
      601 actttttcgg caacggcttt gacggcgcgc tcttcaatca gcgggcccat ttccagcgcg
      661 cctgcttcgg cttcggcagg gttgccgtag cgtacgcctt tcatggcggc ggtcattttt
      721 tcaatgaatg cgtctttcag actgctgtgg acataaacgc gctcggcgca gttgcagatt
      781 tgaccagtgt tgccgacgcg cgaagccaag atggatttca ctgccaagtc caaatccgcg
      841 tctttcaaaa cgatagcagg tgctttgccg ccgagttcca gcgagacttt ggtgatgttg
      901 gcagaagcgg cttccatcac ttggcggccc gcttcaacgg agccggtcag gctgaccata
      961 tcgacttgcg gatgggcgga caaggcattg ccgatttccg cacccggacc gttcacaacg
     1021 ttgaacacgc ctgctggcag tccgaccgca tcgacgattt cggcgaagat gtggcagttg
     1081 atcggggtta cgctgctggg tttgacgacg atggtgttgc ccgttaccaa agcggggccc
     1141 attttgcggg caatcaggaa gaaggggaag ttccacggca aaatgccggc gattacgccc
     1201 agcggacgtt tgaacaataa aatattttcg cgcgggcggt cgctttggat gatttcgcct
     1261 tcgtaacgac gcgcccattc ggcttggtaa tcgagatagt cggcggtaaa catgacttcc
     1321 acgcgcgcca agtctttggt tttgccgcct tcggcaacga tggtgtcggt cagctcgtcg
     1381 gcgcgtccgc gtatgccttg ggcgattttg cgcaaatacg cgccgcgttc gaccgcaggc
     1441 aggcgttccc aagccggttg cgccgcgcgt gccgccgcca cagctcggtc aacgtctgct
     1501 tttccgcctt tgggttcgcg ggcgatggtt tcttcggtgg atgggttcaa tacgtcgcgc
     1561 cattcgccgt tgaaatcgtt ttcaaagcgt ccgttgatgt acatggccaa ttgtttcatt
     1621 tcgggttctc cagttttgta gtcagatgta gtcttagttt attcccaaat aaattgccgt
     1681 acaagtttct tttgaaatgt ttagacgaga agctactatg tctgcatatt gttctgctaa
     1741 tttatattca ctcgaatgca actgccgatt attccaatct gcattcgcat cagtaaaagc
     1801 cccagcattg ccgcctgtgg cataaccaat cgctaaactg ccaacggtat ccacaacagg
     1861 tttcaggctg cctgaatttc tctaataaat taaacactta ttctatcagc cgttaatgta
     1921 accaaacaat tatcaagata agagtagttt actaaatact catcttcttc aaattgtata
     1981 ttttgtaggg ttagatgatt attttttaat tctgccgtta tttcatactt gaaactatca
     2041 tctatttttc taaatccaat atttgaagtt gatttatcca gtatgtaatc attaaagatt
     2101 tcataggata attccacttc ataaaattga ttttcaaata ttttgaatgg gcagtatgat
     2161 ataaaacaat gtagttcata accatttatt tcaatcaata ctgcctcttc aatggtgtta
     2221 tctaactttt tgattttaca tttatataac attttatctt gcctttagat aaattgtttt
     2281 gttttttgga ttgttattca aagttctcca aaagtctttt cctgattttg tatgtatcgt
     2341 ggtaacacta ccattttcat tggttccaac aaaaataaat ttagattgac ctttggaatc
     2401 agtacccaaa aactgaacat atccagacat gcggatttcg ggttttaaat caaatgcggc
     2461 ttaccggttt tcttttttgg tttccgccgg cgtgctgccg aaatattgcc tgaatgcctg
     2521 agtaaagctg gaaacgtggc ggtagccgca taaatacgcg gtttcgccta cgcttttgcc
     2581 cccgttttgc aacagataga gcgcgtgctg catttgtttg tggtgcagcc actcgcttgc
     2641 cgtaatgccg aaatggtcgc gcatacggcg ttgcagcgtc ctttcgctga tgttcagcgc
     2701 gtctgtcagc cggttgactt ggtgtgcgcc gccgacaaac gcggcattca gggtgcggct
     2761 gaagtcttca gacggcatag cgtctgcttc cgccgtttgc cccgccgccg gcccgatgcc
     2821 gtctgaaacc gtgtcccaca agtccgacag cagccgcaaa acgtccgcct cgcggcgcaa
     2881 tgtttcgccc aaatgcccct ttgggacggc tttcaggcag gatgccgcca agccgcgcag
     2941 gttcgggggc aaatcccata tcctgaccgg ttcgcggtaa agcaggggtg cgaaacgcgc
     3001 gtattccgga cgcagcagcc attgttccat acctttaatg gtcattttga ccgttttgcc
     3061 gcctcggtaa agatagcggc tgaacaggac ttcttccccg acagcaatta ggacaatctt
     3121 gccgccgtcc gcatcgattt ggaagcggca gcggttgatg ccgaagtcca aactgccttc
     3181 cagcaagagc acgaacgaca cataaggttc ggcgaggcgg ctgctgcaaa aatcacaccg
     3241 tgccgttacc gtgccgccgt ggatggaaat gccgttggac aaagtatcga aacggtagct
     3301 gccctccaca tagctgccgc cgccgctttt gaccagattg acgaaccggc tgctgttcaa
     3361 atgttctgct ttttccatag ttttctgata aaggttatca tttgaaagat aacatttttc
     3421 gccacagcaa acaatccgcc gccacaacgg cattttcaca acacttgccg gccgtatgcc
     3481 gtctgaaaat tgcccgtctt cccgataagc cttaaaatac acgccgtcaa acctaccgtc
     3541 cgtccgtaca tgagcccatc cccctttatc gaaatgaaag acgtcgcttt tgcgtatggc
     3601 gaccgcccga ttctgaacga catcaatttc agcattccgc aaggcaattt tgccgccgtg
     3661 atgggcggtt cgggcagcgg caaaaccacg ctgatgcggc tgattacagg gcagattcgt
     3721 ccgcagtccg ggcaggtttt gattgaaggg cgggatttgt cgggcttttc ggctgacgaa
     3781 ctctacgaac accgccgccg tatgggcgta ttgttccaac acggcgcgct gtttactgat
     3841 ttgtctgtat ttgacaatat cgcttttccg atgcgcgaac tgacgcagct gccggaagcg
     3901 gtgattcgag atttggtttt gttgaaactg aacgcggtcg gtctgcgcgg tgtagaaaac
     3961 ctgatgccgt ccgagttgtc cggcgggatg tcgcgccgcg tcgcgcttgc ccgcacgatt
     4021 gcgctcgacc ccgaaattat gttgtacgac gaaccgttta ccggtctcga tccgatttcc
     4081 ttgggcgtga ttgcccactt aatcagccgc gtcaacaagg ctttgcgttc gaccagtatt
     4141 atggtaacgc acgacattga aaaatctttg gaaatcgtcg atcaggtgat tttcttggcg
     4201 cacggcgaaa ttatgttctc cggctcgccg caggaaatgc gcgaactgga ttcgccttgg
     4261 gtgcgccagt ttgtcggcgg cttggcagac ggtcccgtag cataccgcta tccggcgcaa
     4321 acgtcgttgc agcaggacct gttgggataa atgagttgat gccgtctgaa accgttgaac
     4381 tttacacata gaaatttatg aactttatcc gttccgtcgg ggcgaaaacc ctcggcctta
     4441 ttcaatccct cggcagtatc acgctgtttc tgctgaacat tctggcgaag tccggtacgg
     4501 ctttcgtccg tccgcgcctg agcgtgcgcc aagtgtattt cgccggcgtg ctgtcggtgt
     4561 tgattgttgc cgtttcgggg ctgtttgtcg gtatggtctt gggtttgcag ggctatacgc
     4621 agttgtcgaa attcaaatcc gccgatattt tgggctatat ggtcgcggct tcgctgttgc
     4681 gggaactggg tccggtgttg gcggcgatcc tgtttgccag cagcgcgggc ggtgcgatga
     4741 ccagcgaaat cggtctgatg aaaacgaccg aacagctcga agcgatgaac gtgatggcgg
     4801 taaaccctgt cgcccgcgtg gttgcgccgc gcttttgggc gggcgtgttt tccatgccgc
     4861 ttttggcttc gattttcaac gtggcgggta ttttcggcgc gtatttggtc ggtgtaacct
     4921 ggctgggctt ggacagcggc attttctggt cgcaaatgca gaacaacatc acgatacact
     4981 acgatgtaat caacggtctg atcaagtccg ccgcgttcgg cgtggcggta acgctgattg
     5041 ccgtgcatca gggcttccat tgcgtcccga cctcggaagg cattttgcgc gccagcacgc
     5101 gtacggtggt ttcgtccgcc ctgacgattt tggcgatcga ctttgtgttg accgcgtgga
     5161 tgtttacaga ttgacaggcc gtctgaaaac gaaacaaaaa acatttggat attcaaggaa
     5221 ctttaatgaa aaagaacata ttggaatttt gggtcggact gtttgtcctg ctcggcgcgg
     5281 cggcggttgc ctttctcgct ttccgcgtgg ccggcggtgc ggcgttcggc ggttcggaca
     5341 aaacttacgc cgtttatgcc gatttcggcg acatcggcgg tttgaaggtc aatgcccccg
     5401 tcaaatctgc aggcgtattg gtcgggcgcg tcggcgctat cggacttgac ccgaaatcct
     5461 atcaggcgag ggtgcgcctc gatttggacg gcaagtatca gttcagcagc gacgtttccg
     5521 cgcaaatcct gacttcggga cttttgggcg agcagtacat cgggctgcag cagggcggcg
     5581 acacggaaaa ccttgctgcc ggcgacacca tctccgtaac cagttctgca atggttctgg
     5641 aaaaccttat cggcaaattc atgacgagtt ttgccgagaa aaatgccgac ggcggcaatg
     5701 cggaaaaagc cgccgaataa acgtatgcca tcatattcaa actaggacac ttaatcatga
     5761 aaaaatcctc cttcatcagc gcattgggca tcggtatttt gagcatcggc atggcatttg
     5821 ccgcccctgc cgacgcggta aaccaaatcc gtcaaaacgc cactcaagta ttgagcatct
     5881 taaaaagcgg tgatgccaac accacccgcc aaaaagccga agcctatgcg attccctatt
     5941 tcgatttcca acgtatgacc gcattggcgg tcggcaaccc ttggcgcacc gcgtccgacg
     6001 cgcaaaaaca agcgttggcc aaagaatttc aaaccctgct gatccgcacc tattccggca
     6061 cgatgctgaa attaaaaaac gccaacgtca acgtcaaaga caatcccatc gtcaataaag
     6121 gcggcaaaga aatcatcgtc cgcgccgaag tcggcgtacc cgggcaaaaa cccgtcaaca
     6181 tggacttcac cacctaccaa agcggcggta aataccgtac ctacaacgtc gccatcgaag
     6241 gcgcgagcct ggttaccgtg taccgcaacc aattcggcga aatcatcaaa gcgaaaggcg
     6301 tggacggact gattgccgag ttgaaggcta aaaacggcag caagtaatcc ataagccatg
     6361 ccgccagtct gaaggcggca tggatttttt cctaccgaac actatgcata cagaactcaa
     6421 caacggaaca ctgcacatca gcggcgacgt aaccgtcaaa accctgaccg gggacgcatt
     6481 tgagcgtttc cggcagcaat gccgtctgaa agaaaccatt gccgtcgatt tcggcggtgt
     6541 caaacgtgcc gattccgcct gcgtgtcgct gctgctcgaa gtgctgcgcg gctgcaaagg
     6601 cagcgtcagg ctgaccggca ttcccgaatc cgtgcgcgcg ctgtccgaac tgtacgaaat
     6661 caaagactgg ctgaaatcat gaaaaaaacc gcctatgcct tcctcctgct gattgggttt
     6721 gcttccgccc ctgcatttgc cgaaacccgc cccgccgacc cttatgaagg ctacaaccgc
     6781 gccgttttca aattcaacga ccaagccgac cgctacattt tcgcccctgc cgcgcgcggc
     6841 taccgcaaag ttgcgccgaa acccgtccgc gccggcgtgt ccaatttttt taacaacctg
     6901 tgcgacgtgg tcagcttcgg cagcaatatc ttgcgcttgg acatcaaacg cgcaagcgaa
     6961 gaccttgtcc gcgtcggcat caacaccact ttcggtttgg gcgggcttat cgacatcgcc
     7021 ggcgcgggcg gcattcccga caataaaaac accttgggcg acacgtttgc ctcgtgggga
     7081 tggaaaaaca gcaattattt cgtgttgccc gtcttagggc cgtccaccgt ccgcgacgcg
     7141 ctcggcacgg gtattacctc cgtttattcg cccaagaata tcgtcttccg cacccctgtc
     7201 ggacgctggg gcacgactgc cgtatccgcc gtcagtacgc gcgaaggcct gctcgatttg
     7261 accgacagtc tggacgaagc cgccatcgac aaatacagct acacgcgcga cctctatatg
     7321 aaagtccgtg cgcggcagac cggtgcaaca cctgccgaag gtacggaaga taacatcgac
     7381 atcgacgaat tggtcgaaag tgccgaaacc ggcgcggcgg aaactgccgt tcaagaagat
     7441 tccgtatccg aaacacaggc agaagcagca ggggaagccg aaacgcaacc tggaacacaa
     7501 ccttaaagcc atgccgtctg aaaatccgtg ttcggacggc attcccttct gtcggaacgg
     7561 acagtccgaa accacagatt gggctataat cccttttttg cgcataccgc gcgacagcaa
     7621 aggaaaaacc atgtacgaag tcaaccgcag cgtattcgtc ctcattccac tcgaaccctt
     7681 ctggaactgg ctgcaaaccc tgccgggcaa ccaccttgac ggactgaccc tcgaagacat
     7741 ccaagccgac gcgaattcct accttgtccg cccgtgcgaa accgccgacg aagtatggga
     7801 cgaaatcgaa acccgttttg aagacatttt cgccgccgaa cttgccgact ggtgcgaaga
     7861 cgaacgcgag tggcctgcgc tcgatgccga cattttcaac gaatggttcg acatccagct
     7921 ttctaccgtc atcaccgacc tcgaacacga accgcttgcc cgcgaagcct tccaacccat
     7981 caacctgaac tgatgaagct gaccgtccgc aactaccatc tcgacggcta cggccatgtc
     8041 aacaatgcgc gctacctcga atttttcgaa gaagcgcgct gggcgttttt tgaagaacgc
     8101 ggactgctgc acgagttggc aggcctcata ctgattgtcg cccgcatcga catccgatac
     8161 agccgccccg ccgtcgaagg cgacgtattg cagttttcat gccgcctgaa aacccccggc
     8221 acgcgccgta ttgtgctgac ccagacgatt acactgccaa acggcaaaac cgccgcagaa
     8281 gccgacatca ccctgatgcc cgtccacgcc gccacacaac gcaccgtcag cctgcccgcc
     8341 acccttgccc gcgcattgga agccttgtcc gaatgaaaaa aatactcacc gccgccgtcg
     8401 tcgcactgat cggcatcctc cttgccatcg tcctcatccc cgacagcaaa accgcgcccg
     8461 ccttctccct gcccgacctg cacggaaaaa ccgtttccaa cgccgacctg caaggcaaag
     8521 taaccctgat taatttttgg tttccctcct gtccgggttg tgtgagcgaa atgcccaaaa
     8581 tcattaaaac ggcaaatgac tataaaaaca aaaacttcca agtccttgcc gtcgcccagc
     8641 ccatcgatcc gatagaaagc gtccgccaat atgtcaaaga ctacggtttg ccgtttaccg
     8701 tcatgtatga tgcggacaaa gctgtcggac aggcgttcgg cacacaggtt tatccgactt
     8761 ccgtccttat cggcaaaaaa ggcgaaatcc tcaaaactta tgtcggcgaa cccgatttcg
     8821 gcaaactcta ccaagaaatc gataccgcgc tggcacaata gcgatgccgt ctgaagcgca
     8881 aaaaaacaga aacgatgttc ctgttttttc agatcatccg ccccgacacg ttcagacggc
     8941 attcaacctt ccggcagatt ctttttcacc actgccggat tgcctgccgc caaagagtag
     9001 ggcggaatgt cttttgtaac caccgcgcct gcgcccacga ccgaaccgcg tccgacggtt
     9061 acgcccgcca ttacaatcac cctgtgcccc ggccagacat cgtcctccaa cgtaatcgga
     9121 cggatttccg tgtagccctc aaagcgtttt ttgaacggtc aaacttgtgg ttatttgaat
     9181 aaaacagaca ttccggctcc atcatcacat ttttgccgac caccagccca cggcagattt
     9241 cacagtttgc cccgatgccc gagccgtcgc ccaaaaccgt atccggaaac acatacgccc
     9301 cgcgttcgat attgaccccg cgtccgatat gcggagaaac ccgccgcgcc aaaaaaccgc
     9361 gcacccgtct gccgacgatg ccgatgccgc gcatatgtga cggcggcaac cccgaaccgg
     9421 caaaacgcag cagcaggaaa aagaattttg atcccatcaa acctccctca aaagaacttt
     9481 ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt acagatagta
     9541 cggcaaggcg aggcaacgct gtactggttt ttgttaatcc actatacatt ttatcgaata
     9601 tcctcttgac gaaacaacag aaaatagcga gaatgccgag cttgtctgta gcataggtac
     9661 attccctatg cccgcttttt tgaaaggaaa tcaaatgaaa caaggtattc acccgaacta
     9721 ccacgaagtc aacgttacct gctcttgcgg caacaaattc gcaaccaaat ccgcgatgga
     9781 aaaagaaaac ttcaatatcg aggtttgctc cctgtgccac ccgttctata ccggcaccca
     9841 aaaaatcgtc gataccaccg gccgcgtgga caaattcaac aacaaattcg gcaacctgtt
     9901 caaacgctga ttgccgcttt gcaagaatta agccctgcca tcatttcgat ggcagggctt
     9961 tttaatattt ggcacactga cttcttatct atatagtgga ttaacaaaaa tcaggacaag
    10021 gcgacgaagc cgcagacagt acaaatagta cggaaccgat tcacttggtg cttcagcacc
    10081 ttagagaatc gttctctttg agctaaggcg aggcaacgct gtactggttt ttgttaatcc
    10141 actatatttt gacggggttg aagttgacat ctacaaggtt taatgtttga atacatttaa
    10201 aaatatattt gaatgagatg ttttatgatt caaaatgttg ttacttcaat aatcctgtat
    10261 tctgggacag ccgtagactt acttattatc ctaatgttat tttttgccaa aagaaaaagc
    10321 agaaaagaca tcattaacat ctatttagga caatttctag gctctgttag cctgatattg
    10381 ctaagtttgc tttttgcatt tgtcttagat tatattccta gtaaagagat tttaggtttg
    10441 ctcggcttga ttccaatttt cctaggcatc aaagttttgc tgttaggaga ttctgatgga
    10501 gaggctattg ccaaagaggg tttgcgcaaa gataataaaa acctgatttt tctagtcgct
    10561 atgattactt ttgcaagttg tggtgctgac aatattggtg tctttgtccc atattttact
    10621 accttaaatt tagcgaattt gatagtggct ttacttacct ttctagtcat gatttatctc
    10681 ttggtttttt ctgcccaaaa attagcacaa gtcccttctg ttggagaaac tttggaaaaa
    10741 tatagcagat ggtttgttgc tgttgtttat ttaggattgg ggatatatat cctggttgaa
    10801 aacaacagtt ttgatatgct atggactgtg ttgggctagg aaaaaatatt atgaaaaaga
    10861 tagtatctgc aaagactgcc atgtgcagtc ttttttgttg ccggtctttt tgtgtctgat
    10921 gccgtctgaa gcagtctctg cacgaccttt gtgcgaatat ttgctacact tggcaatccg
    10981 tccatttttt ctcacgctta tgctgaccta taccccgccc gatgcccgcc cgcccgccaa
    11041 aacccacgaa aagccgtggc tgctgctgtt gatggcgttt gcctggttgt ggccgggtgt
    11101 attttcacat gatttatgga atcctgccga acctgccgtc tataccgccg tcgaagcact
    11161 ggcaggcagc cccaccccct tggttgccca tctgttcggt caaaccgatt tcggcatacc
    11221 gcccgtgtat ctttgggttg ccgccgcgtt caaacatttg ctgtcgccgt gggctgccga
    11281 ctcatacgat gccgcacgct ttgcaggcgt gtttttcgcc gttgtcggac tgacttcctg
    11341 cggctttgcc ggtttcaact ttttgggcag acaccacggg cgcagcgttg ttttaatcct
    11401 tatcggctgt atcgggctga ttccgaccgt acactttctc aatcccgctg ccgccgcctt
    11461 tgccgccgcc ggactggtgc tgcacggcta ctcgctggcg tgccgccgtg tgattgccgc
    11521 ctcttttctg ctcggtacgg gttggacgct gatgtcgttg gcagcagctt atccggcagc
    11581 atttgccctg atgctgcccc tgccagtgct gatgtttttc cgtccgtggc aaagcaggcg
    11641 tttgatgttg acggcagtcg cctcgcttgc ctttgccctg ccgctgatga ccgtttaccc
    11701 gctgctcttg gcaaaaacgc agcccgcgct gttcgcgcaa tggctcgacg atcacgtttt
    11761 cggtacgttc ggcggcgtgc ggcacgttca gacggcattc agtttgtttt actatctgaa
    11821 aaacctgctt tggtttgcac tgcccgcgct gccgctggcg gtttggacgg tttgccgcac
    11881 gcgcctgttt tcgaccgact gggggatttt gggcgttgtg tggatgcttg ccgttttggt
    11941 gctgctcgcc gccaatccgc agcgttttca ggacaacctc gtctggctgc tgccgccgct
    12001 tgccctgttc ggcgcggcgc aactggacag cctgaggcgc ggcgcggcgg cgtttgtcaa
    12061 ctggttcggc attatggcgt tcggactgtt tgccgtgttc ctgtggacgg gttttttcgc
    12121 tatgaattac ggctggcccg ccaagcttgc cgaacgcgcc gcctacttca gcccgtatta
    12181 tgttcctgat atcgatccca ttccgatggc ggttgccgta ctgttcacac ccttgtggct
    12241 gtgggcgatt acccggaaaa acatacgcgg caggcaggcg gttaccaact gggcggcagg
    12301 cgttaccctg acctgggctt tgctgatgac gctgttcctg ccgtggctgg acgcggcgaa
    12361 aagccacgcg ccggtcgtcc ggagtatgga ggcatcgctt tccccggaat taaaacggga
    12421 gctttcagac ggcatcgagt gtatcgacat aggcggcggc gacctgcaca cgcggattgt
    12481 ttggacgcag tacggcacat tgccgcaccg cgtcggcgat gtacaatgcc gctaccgtat
    12541 cgtccgcttg ccccaaaatg cggatgcgtc gcaaggctgg cagacggtct ggcagggtgc
    12601 gcgcccgcgc aacaaagaca gtaagttcgc actgatacgg aaaatcgggg aaaatatatt
    12661 aaaaacaaca gattgagccg aatttctgga ttaagtgccg gaaatcgcgt ataattgcgc
    12721 gattaaacct ttataaatca gccgttttgc aggcatcaca caggagcgac aattattatg
    12781 atgaccctct attccggcat tacctgcccc ttcagccacc gctgccgctt cgttttgtac
    12841 gaaaaaggta tggattttga aatcaaagac gtcgatattt acaacaaacc cgaagacctc
    12901 gccgtcatga atccgtataa ccaagttccc gtgctggtcg agcgcgattt ggtgctgcac
    12961 gagtccaaca tcatcaacga atacatcgac gaacgcttcc ctcatccgca gcttatgccc
    13021 ggcgatcccg ttatgcgcgg ccggggccgg ctggtgctgt accgtatgga aaaagaattg
    13081 ttcaaccacg tccaagtgtt agaaaacccc gccgccacca acaaggaaca ggcaaaagcg
    13141 cgcgaagcca tcggcaacgg tctgaccatg ctcgcccctt cgttcagcaa aagcaaatac
    13201 atcctcggcg aagatttttc tatgattgat gtcgcccttg ctccgctgct gtggcggctc
    13261 gaccactacg atgtcaaact gggcaaaagt gccgcgcagc tgctcaaata cgccgagcgc
    13321 atcttccagc gcgaagcctt tatcgaagca ctgacacccg ccgaaaaagc catgcgcaaa
    13381 taagtccgaa atgcttgcaa aacccgccgt tttgcaggca ttttcctatt ttggcgtaca
    13441 acacggaacc attatgccca cttccaccaa accctacatc ctccgcgccc tctgcgaatg
    13501 gtgcagcgac aacagcctca caccgcacat ccttgtctgg gtcaacgaac acacgcgcgt
    13561 ccccatgcag tacgtccgcg acaacgaaat tatgctcaac atcggcgcga ccgccacgca
    13621 aaaccttcaa atcgacaacg attggatcag tttttccgcc cgtttcagcg gacaggcgca
    13681 cgatatatgg atacctgtcg gacacgtcct cagccttttc gcacgggaga ccggcgaagg
    13741 tatggggttt gaattggaag cgtatcgccc cgacacgccg cctgaaaaca cctctgccga
    13801 aaccgcgccc cggcccgcca aaaaaggctt gaaattggtc aaataaatct atgccgtctg
    13861 aacggaatcg tgtttcagac ggcattttgt ccgatggggc gcaaacggaa tccgtttatc
    13921 ggcaaaaccc gtttcggtgt atcgaaaccg tgttgcccct gccgattcga tattgggaca
    13981 ttgaaaatgc cgtctgaacc tgcgatacgg gcttcagacg gcatttttcc ttatattcat
    14041 acaatcaggc ggtcagtacg gctttcagaa ttttgttgac ttcgcccata tcggctttgc
    14101 cggcgaggcg ggttttcaat acgcccatca ctttgcccat atccgccata cctgccgcgc
    14161 cggtttcggc aacggcggca tcgaccgcag tacggatttc cccggcggag agcatttggg
    14221 gaaggtagcg gtgcagtacc tcgatttcgg cgttttcttt gtctgccaag tcctgacggc
    14281 cggcttcagt gtagattttc gcgctgtctt ttcgctgttt gaccattttg gtcaggatgg
    14341 cggtgatttt ggcatcgtcg gcttcggtgc gttcgtccac ttcaaactgt ttgacggcgg
    14401 cgttgatgag gcggatggtg ccgagggaaa cttggtcttt ggcgcgcatc gcggttttca
    14461 tgtcttcggt aaggcggatt ttcaggctca taatgtcctc gctgggatgt cggatggaaa
    14521 cggcgggttt cgatgccgtc tgaaaagcaa aacacaccgc agagcaggtc ttgcggtgtg
    14581 tcctcatatc acagggctga cctgtaatct gtacttgaac gtttagtaca ttttgggcgg
    14641 cagttgttgg ctgcgcaggc gtttttgcag gcgttttacg gctgccgctt ttttgcgttt
    14701 gcgttcggta gtcggttttt cgtaggcttc gcgggcgcgc agctcggtca gcaggccggt
    14761 tttttctacg gcgcgtttga aacggcgcat agcgacttca aagggttcgt tttcttttac
    14821 gcggattgca ggcattttat ttcctttaat aaattctgtt gttgcatctg cccatcatat
    14881 cggtggaaag ggtagggcag acggtgttga aggtttaccg tatcgctgtg ccttgcggcg
    14941 gcgggatacg cgccgtgacg gaaacatcac ctcctaacgg gtcggcttgt gccgtgtcaa
    15001 gctgcttgaa aaagcaggaa aaacaatttt cggattgtct tatatttatg gtttgccgtc
    15061 aatgccgttc gggataaaaa tgccgtctga aagaccgggc gggtttcaga cggcatcggt
    15121 acgtcagcgt gcaggaacaa tgcccatacg ctgtttgagc gggatgtgcg gcgcgccgaa
    15181 gagggcggcg tagtaggcgg cattggtcat cacttttttg acatagtcgc gcgtttcgga
    15241 aaacgggatg gtttcggcat ataccgcgcc ttcgaggggc gtgtccgcct gccatcggcg
    15301 cgccctgccg ggaccggcgt tatagcctgc ggtggcgagg acttcgttgt tttgcaggcg
    15361 gcgtttggtg tccgccatat accacgtccc catacggata ttgccgtcgg cggtgtaaaa
    15421 ttgtgcggca tccataccga ttttgccggc gatttcgcgc gcggtggcag gcataacctg
    15481 catcagcccc tgcgcgccta cgcgggattg cgcgcctata acgaagcggc tttcctgacg
    15541 aatcagccca taaacccaag ccggatcgac attaacattt tgcgcgtggc ggattaccgt
    15601 gtctttaaac ggcgaaatat agcgcaaggt gtagttgagt ttgcggtcgg tgcgttccgc
    15661 gctgttgacc gccatatcgt aaaaaccgtg gtcgaacgcg gtttgcgcgg cggtcagcag
    15721 cttgtcttcg tcaaagccgc gtgtggcaaa acgccattcc gcctgagcct gacggcgcat
    15781 ttttgcatca ccggcagatt ggctgttttg gaacagtacc agtgcgcgtt tgactgcacc
    15841 gtcttccgcc atgcggcgga cgctgtttgt gtcggcatcg ggcacattgt tgcgcgtatc
    15901 gattttccga cccaactctt cccctgccag tacggcataa aaattcctgc ccgtcgctgc
    15961 cgcctgttca tagagttttg ctgcctgctg caagttgccc agcgcagcat ggctgcgtgc
    16021 cagccagtag agccaggtcg ggcttttttg cagtttttcg ggcatatgcg agataacgga
    16081 agccagctcg tcccaacgtc gggcgcgcag ggcggcgcgg gcgtaccact cgatttggtc
    16141 gtcggtcagt tggcggcggt cggaaacttt gccgtaatag tccaaggcgg caggcacatt
    16201 gaggctttgc gactgataat gccccaatac gccccacgcg aaactgcgtt gttcggggct
    16261 taaaccgctt tccatttcgg acagcagggc ggcggcattc ggcgatttac gcgcctcttt
    16321 gccgatgacg ttcaacaggg cgtattcgcg cgaaccttgt gtgctgccgt caaacgggct
    16381 gcccaatgcg gcggcaaggt tgcgtgcgtc tgtggtttgg ctgttggcca acagtccgcg
    16441 aacgcgcctc caggcattgt tgctgtccaa caagccggat gcggctgcct gttccaacag
    16501 tttggtgcag cccgaaggca gtttgcccgt atttttaacc agttcggcgg cacgcgtata
    16561 gtcgctgtgt ttcaagtcgg cataacacac gacttcttgg gcgcgccctt caggtttgag
    16621 tttggcatat tcctgtgcaa acagcgtcca ctgtctgcgc gcgcccaaag acttcagcca
    16681 ctcgttgcgg acattttccg ccatcgcact gtcgccggcg ttttctaaat aggcggcgac
    16741 ggcggcatcg tttttctgtt tcactgcatc cagtgcggac gggtagccgc cgtaatctgc
    16801 cagcgttttt ccttcgggtt cgacagggcg ggtgggaacg cttgccgaaa ggtcggcagc
    16861 ttctatattg tctgccgggg tcttgccggc tggcagtgtg tttgtcgaag aacacgcggc
    16921 aagcactagg gtcgccagca gcggcaggga atgcttcata gagggtaggt acatcggatt
    16981 tccttaagaa tcggaaccct gaacggtcag ggttggaaaa gacaaaatgc cgtctgaaca
    17041 ggcgtttgcc cgaattatat gccgaaactg caccgccttt ggaatgtttc cgacataatt
    17101 tatagtggat taacaaaaac cagtacggcg ttgcctcgcc ttagctcaaa gagaacgatt
    17161 ctctaaggtg ctgaagcacc aagtgaatcg gttccgtact atttgtactg tctgcggctt
    17221 cgtcgccttg tcctgatttt tgttaatcca ctatatattt ttcaattatt tgccgtttta
    17281 gtgcgaaccg ctgcctttgc ctgtttcaga cggcattgtc cgaaatggtt gcccgcttcc
    17341 tgccttattg acaaaaaaac gctttcccga taatatccta cgaaaattaa cctgccgatt
    17401 tgacacagct tgcgggcata acagctaaag cgttccgaca atttcagctt tatcttccgc
    17461 gcccgttgtg tccgacatcg ggctttgttg tatgggaaag acaatgatta ttttggacaa
    17521 ggtttccaag cattaccaaa cgcgcgacaa gacccgtttt gccgctgtcg agccgaccag
    17581 cctcgaaatc cgcgacggtg aaatcttcgg gctgatgggt tattcgggtg caggcaaatc
    17641 caccctgttg cgactgatta acctgttgga acgtcccgac acgggcaagg tcaacgtctg
    17701 tggacaagag ctgaccgcgc ttgatgccgc cgcattgcgt caggctcggc agaatatcgg
    17761 catggtgttt cagcagttca acctgttgaa caaccgcacc gtcgccggca acgtcgcctt
    17821 cccattggaa atcgccggat ggccgtctga aaaaatcaaa gcgcgcgttg ccgaatgtct
    17881 cgaaatcgtc ggactgaccg aacgcgccgg tcactatccc gcccagcttt ccggcggaca
    17941 aaaacagcgc gtcggcatcg cccgcgcgct cgcatccaag ccgtaagtca tcctcgccga
    18001 cgaacccact tccgccctcg accccgccac cacgcgcagc gtcttggaat gtttggaaga
    18061 catcaacaaa cgcttcaacg taaccatcgt catcgtaacc cacgaaatga gcgtcatcag
    18121 acgactgtgc gaccgagccg ccctgttgga taagggaaaa gtcgtcgaaa tcgtcgaagt
    18181 acgcggcaac caaatccacg cccgatccga catcgggcgc gaactcatca gagaggactg
    18241 aaatggcaga cctgaccttc caacaagccg tttccaacat catcggtatg aaaggcgaaa
    18301 tcgtccgcgc attgggcgaa accttcgtga tggtcggctt gtccaccaca ttcgccgtcg
    18361 ttttcggcac gttgctgggc gtgttgctct tcgtaacttc cagccgccaa ctgcattaca
    18421 acaagccggt gaacttcctg ctcgacaacc tcgtcaacct catgcgcgcc ttccccttcg
    18481 tcatcctgat gattgccatg atacccgcga cccgcaccat cgtcggcagc accatcggtc
    18541 cggttgccgc ctcgctggtg ttgagcgtgt cgggattgtt ttattttgcc cgactggtgg
    18601 aacaaaacct gcgcgaagtc cccaaaggcg tgattgaagc cgccgccgcg atgggtgcgc
    18661 ctccgattgc catcgtctgc aaagtcctct tgaacgaagc gcgcgcgggc atggtttcca
    18721 gcattaccgt gcttgccatc gggcttttgt catacagcgc ggcggcaggg atgataggcg
    18781 gcggcggctt gggcgacctc gccatccgct acggctacta ccgctaccaa accgaagtca
    18841 tcgtcttcat cgtcgccctc ctcgtgctgc tcgttatcct gattcaaagc accggcaacg
    18901 cgttggcgcg gaaactcgac aaacgttgaa cccaaaatgc cgtctgaacg ccaaaacccc
    18961 caccgctatc cgaaaaatgc tataaaatcc ccctgttcgc ggcaaatgcc gtctgaacgc
    19021 cgaatccgga cggcaggact ccctgcccgt catttttgtt tgaaactgcc acaacatcag
    19081 gagaaaatat gaaaaccttc ttcaaaaccc tttccgccgc cgcactcgcg ctcatcctcg
    19141 ccgcctgcgg cggtcaaaaa gacagcgcgc ccgccgcatc cgcttctgcc gccgccgaca
    19201 acggcgcgga gaaaaaagaa atcgtcttcg gcacgaccgt cggcgacttc ggcgatatgg
    19261 tcaaagaaca catccaaccc gagctggaga aaaaaggcta caccgtcaaa ctggtcgagt
    19321 ttaccgacta tgtacgcccg aatctggcat tggctgaggg cgagttggac atcaacgtct
    19381 tccaacacaa accctatctt gacgacttca aaaaagaaca caatctggac atcaccgaag
    19441 tcttccaagt gccgaccgcg cctttgggac tgtacccggg caagctgaaa tcgctggaag
    19501 aagtcaaaga cggcagcacc gtatccgcgc ccaacgaccc gtccaacttc gcccgcgtct
    19561 tggtgatgct cgacgaactg ggttggatca aactcaaaga cggcatcaat ccgctgaccg
    19621 catccaaagc ggacatcgcc gagaacctga aaaacatcaa aatcgtcgag cttgaagccg
    19681 cgcaactgcc gcgtagccgc gccgacgtgg attttgccgt cgtcaacggc aactacgcca
    19741 taagcagcgg catgaagctg accgaagccc tgttccaaga accgagcttt gcctatgtca
    19801 actggtctgc cgtcaaaacc gccgacaaag acagccaatg gcttaaagac gtaaccgagg
    19861 cctataactc cgacgcgttc aaagcctacg cgcacaaacg cttcgagggc tacaaatccc
    19921 ctgccgcatg gaatgaaggc gcagccaaat aaggcagtcg tataaaatga tgccgtctga
    19981 accgtatccg tgttcagacg gcatttttgt cctttaatcc gccattccct gccattccgc
    20041 cgaatccggc gtatcgattc cgaacagcga caaagcgtgt gcaacactgt gcgccactat
    20101 gtcgtccgcc gtctgcggtt tgcggtacat cgcaggaaca gggggaaaca ccacgccgcc
    20161 catttccgtt acccgcttca tattgtccaa atgggcaagg ttcagcggcg tttcgcgcac
    20221 catcagcacc agccgccgcc tttccttcaa aaccacatcc gccgcacgcg tcaagaggtt
    20281 gtcgccgaag ccgtgcgcga ccgaggcaag cgtccgcatc gaacaggggg cgaccagcat
    20341 cccgtccgtt ttaaacgtac cgctggcaat gcacgccccg atattgccga tcggatgcac
    20401 gaagtccgcc aaggcatata cctcgtctct cgcataagcc gtttccgaag cgcgcgccat
    20461 ctccgcacct ttcgatacca caaggtgcgt ttcgatatct tgcgcgcgta aaagttccaa
    20521 agccttcacg ccgtattgga aaccgctcgc cccactgatg ccgattatca aacgccgtac
    20581 catcatccgc ctttcccata aaaccgcctg caacggcaaa ccagctatta tagtgaaaaa
    20641 acagaaatcc gataaacacg gatacaaatt gtcggcaaca cccaatatcc gataaaatat
    20701 ccgatttaac atcctatctg aaataggcac gggagggcgg tatggcaaaa gtaaaaggtg
    20761 gattggggcg cggtttggat tcgctgctcg ccaacggcgc agacaacagc agcggcgacc
    20821 gattgaccac ggttgcggtt aaagatatcc ggcccggccg ctatcaggcg cgtgttcaaa
    20881 tcgatgacga agccttgcag gagctggcgg attcgattaa ggcgcaaggc gtgatacagc
    20941 ccgtcatcgt gcgcgaacac ggactgtccc gatacgaact gattgcgggg gagcggcgtt
    21001 ggcgtgccgc acagattgcc ggcctgaccg aaatccccgc cgttatcaaa accatcagcg
    21061 acgaaaccgc attggcaatg ggtttgatcg aaaacctcca gcgcgaaaac ctcaacccca
    21121 tcgaagaagc acaaggcttg aaacgccttg ccgacgagtt cggactgacc cacgaaacca
    21181 tcgcccaggc cgtcggtaaa agccgaagcg cgatttccaa cagcctgcgc cttttaagcc
    21241 tgcccgaacc cgtgcaggaa atgctttacc aacgccgcct cgaaatggga cacgcccgcg
    21301 cattgctgac cctgcccgtc gtcgaacagc tcgaattggc gcaaaaggcc gtcaaaaacg
    21361 gctggtcggt gcgcgaagtc gaacgccgca gccaagccgc ccttcaaaac aaacgtcccg
    21421 agcccaaaaa gactgccgcc gccgacatcg gccgcctgaa tgatttgctg actgaaaaac
    21481 tgggtgtcaa cgccgaaatc aaaaccgcca accataaaaa aggcaaaatc atcctgcatt
    21541 tcgacacgcc cgaaaccttc gaccatatct tgaagcaatt gggcatagat taccgacctt
    21601 aattttgcgg gatataccgt ctgaaatata gagaatagct ttccagattt taagtgggaa
    21661 atataattct attgacattt tttctgcttc acgtaagaat cgttttcctg ttttcatttt
    21721 taattttcga ggaaattatg aacacacgca tcatcgtttc ggctgcgttc gttgcgttgg
    21781 cattagcagg ttgcggctca atcaataatg taaccgtttc cgaccagaaa cttcaggaac
    21841 gtgccgcgtt tgccttgggc gtcagcccaa atgccgtaaa aatcagcaac cgcaacaatg
    21901 aaggcatacg catcaacttt accgcaactg tgggtaagcg cgtgagccaa tgctatgtta
    21961 ccagtgtaat cagcacaatc ggcgttacca cttccgatgc aatttgtttg ggaggcggaa
    22021 cgcacaaagg caaaagtcaa tgcaatgctt tgcttaaagc ggcaggccgt tgctaatcct
    22081 ttattcggaa aaggtcgtct gaaaatattt tcagacgacc ttttatttat tgagcaaatt
    22141 ccccaaactg ccgacaatcc gaccgataaa ctgctacaat ttcgcgttga ttttttatta
    22201 tttccacgta aagcaaacaa atcacgtaag aacattaaaa atgtaaagaa attgcggaca
    22261 aactccttaa tatcctgtaa ttatatgtaa tgtgtgttag aatgacacct gtcgccgtta
    22321 ttcaggcggc gagcgcggcg gattgggata cgttgtttca caaagattaa ctgtcgcttg
    22381 acgtaataag gcttctaaat tatattagcc gcgccccaaa tccgaggcgg aaacattccc
    22441 tttatgaagc agattatcat cctacaatcc gccgtattgt ccatatgcgc cgcagttgcc
    22501 tttgctgtat ggggttttgc cggattcctt tcggcggtcg gtggcggttt gtcctacctg
    22561 cttcccacgt ttgttgcagt tttactttta aaacttttca ggggaaaccc tttcctgcaa
    22621 agcaggatgt tcgtcttcgg agagatttta aaagtagtgc tgtcgctgtt gtccatgctc
    22681 gccgtatttg ctgtatggca tcaatcgttg gtgttcgccc cgtttctgat ggggctgctc
    22741 ggtgtcagcc atttggtttt tttagtattg ttgagagtga aagattatgg caggtgaaac
    22801 cattaccgct gccgactaca tcaagcacca cttgcagagc ttgaccagtt tgtcggatgt
    22861 tactcagggt cagggactga aaaacattgc tgatttttcg tttattaacc ttgatgccgt
    22921 cttttttgcc gtcctgttgg gcgtaatcgg cagcttcctg ttgtggcgcg gtgcgaaaaa
    22981 agcaacggca ggcgttcccg gacgtttcca ggctgccgtg gaaatcttgt ttgagtttgt
    23041 ggacgatatg tgtaagagca tcattcacaa cgaaaagtct agaaaagccg tcgcgccgtt
    23101 gggtctgacg ctgtttgtct ggatttttct gatgaacgcg atggatatgc tgccggtcga
    23161 tttgctgccg atggtatggc agggcattac cggcaaccat cacgccctgc tgcgcgtcgt
    23221 accgactgcc gatttgaaca ccactttggc actcgctgtc ggcgtgttgc tgatttgtat
    23281 ctattacaac atcaaaatca aaggattggg tggctggttt cacgagttgt tcagcgcgcc
    23341 gttcggtgca aaactcgcac ctgcgaactt tctgttgaat ttggtcgagt ttctttctaa
    23401 aaccgtatcc cacggtatgc ggttgttcgg caatatgtat gcaggcgagt tggtattctt
    23461 gttgatagcc ttgcttggcg gtgcttgggc ggcttccggc agcgtcgaag tcatggatcc
    23521 gattctgttt gtattccaca ttattgccgg tttggcatgg gcgattttcc atattttggt
    23581 gattaccctg caggcgttta ttttcatggc gttggcgttc gtctatatcg gacaggcaca
    23641 tgatgcgcac tgattttcct aagtagtttg tttttgtagt ttaacctttg tttctttaag
    23701 gagtttaaaa tgggtttgat tgctatcgca tgtggtttga tcgttgcatt gggcgcactg
    23761 ggtgcctcta tcggtatcgc aatggtcggt tctaaatatt tggagtcttc tgcccgtcag
    23821 cccgaactga tcggtccgct gcaaaccaaa ctgttcctga ttgccggttt gattgatgcc
    23881 gcattcttga ttggtgtcgc cattgcactg ctgttcgcct tcgttaaccc gtttgcaggt
    23941 gcataatcag gacggattcg tccgttccgt acaggcgcag cctgtttgat taaccccaat
    24001 acgaaggtta agtaacgtga atatcaatgc aacattattc gctcaaatca tcgtcttttt
    24061 cggtttggta tggtttacca tgaaattcgt gtggccgccg attgcaaaag ctttggatga
    24121 gcgtgccgca aaagtcgccg aaggcttggc agctgccgag cgtggtaaaa gcgatttcga
    24181 gcaggctgaa aaaaaggttg cagaactctt ggcagaaggg cgtaatcagg tttccgaaat
    24241 ggttgccaac gccgaaaaac gtgccgccaa aattgtcgaa gaagccaaag aacaggcttc
    24301 ttccgaggcg gcgcgcattg cagctcaggc aaaggccgat gtggagcagg aattgttccg
    24361 cgcacgcgaa tccctgcgcg agcaggttgc cgtgttggct gtcaaaggtg ccgaatctat
    24421 tttgcgcagc gaagtcgatg cttccaaaca cgcaaaactg ctcgataccc tgaaacagga
    24481 gctgtaatct tatggcagag ttcgcaacga ttgccagacc ttatgcaaag gcattgttcg
    24541 gtctggctca ggaaaaaagc caaattgagt cttggttggg cggactggaa aaacttgcgg
    24601 cggttgttca ggaagggaag gtggcttcat tgattgaccg tcctgaaacg aatgcttcag
    24661 aaaaagcaga tatcctcatc gatttggtcg gtttgaaaga caaggagttg aaaaactttg
    24721 ttatcgtctt ggccgggcag aaacgtttgt cgatattgcc ggaagtatat gctcaatatc
    24781 aagacttgac cttatcattc aaccatatca aatctgccgt catttacagt gcctatccgt
    24841 tgaccgacaa acaggtcggc gagttggcgc aaatgctgaa taagcgtttc gacagcgagc
    24901 tgaaaatctc tgtcgaaatc gaaccggagc tgattggcgg cataaaagtt gaagtgggtg
    24961 atcaggtttt ggatttgtct gtacaaggca aattgagtgc cttgtacacg actatgacga
    25021 attaggagag ttttcatgca gcttaatcct gctgaaatta gcgatttgat taaagccaag
    25081 atcgaaaatc tgtctgtaaa tgccgaagtg cgtacccgtg gtacggtgat ttcggtaact
    25141 gacggtatcg ttcgcatcta tggtttgtca gatgcaatgc aaggtgagat gctcgaattc
    25201 ccgggtaaca ctttcggctt ggcgatgaac ctggagcgcg actccgtcgg cgccgtagtg
    25261 ttgggcgagt acgaacatat taaagaaggc gacacggtta cctgtaccgg tcgtatcttg
    25321 gaagtgccgg tcggacgcga actggtcgga cgcgtcgttg atgcattggg tcggcctatc
    25381 gacggcaaag gcccgattaa tacaactttg accgccccta tcgaaaaaat cgcaccgggc
    25441 gtgattgcgc gcaaatcggt tgaccagccg atgcaaaccg gtctgaaggc gattgactct
    25501 atggttcctg tcggtcgcgg tcagcgtgag ttgattattg gcgaccgtca aaccggtaaa
    25561 acggccgtag cattggatgc catcgtcaac caaaaaggta cgggtgttat ctgtatctat
    25621 gtcgctatcg gtcaaaaagc atcttctatt gccaacgtgg tccgcaaatt ggaagagcat
    25681 ggcgcgatgg aacacacgat tgtggttgct gcaactgcat ctgaagcggc ggcattgcaa
    25741 tatatcgcac cttactccgg ttgtacgatg ggtgaattct tccgcgatcg tggcgaagat
    25801 gccttgattg tttatgacga tttgtccaaa caggctgtgg cttaccgtca aatttccctg
    25861 cttttgcgcc gtccgcccgg ccgcgaagcc tatcctggcg atgtgttcta cctgcactcc
    25921 cgtctgttgg aacgtgcggc acgtgtcaat gaacacgaag tggagaaatt gaccaacggc
    25981 gaagtaaaag gcaaaaccgg ttctctgacc gcgttgccga ttatcgaaac ccaagcgggc
    26041 gacgtatctg ctttcgttcc gactaacgtc atttcgatta ccgacggtca gattttcttg
    26101 gaaaccgacc tcttcaacgc cggtatccgt cctgcaatca atgccggtat ttccgtatcc
    26161 cgcgtaggcg gtgctgcaca aaccaaagtg attaaaaagc tgggtggcgg tatccgtttg
    26221 gcgttggcac aatatcgtga attggcggcg ttctcgcaat ttgcatccga tttggatgaa
    26281 gctacgcgca aacaattgga gcatggcgaa gtcgtaaccg aactgatgaa acagaaacag
    26341 ttcagtacgt tgaatacggc tgaaatggct ttgacccttt gggcaatcaa caacggttcg
    26401 tattctgatg ttccggttgc caaagctctg gcttttgaat ctgaattttt gagctttgtc
    26461 cgtacccaac atccggaagt tttggaagcc gtcaatgctt caggtgcaat gtccgacgag
    26521 agcgagaaaa cgcttgaagc agccatgaaa tccttcaaat cttcttacgc ctatcaggca
    26581 taaggcttga agtgaaagga gtctgaaatg gcagtaggaa aagagattct caccaaaatc
    26641 cgcagtgttc agaataccca aaagatcact aaagcgatgc agatggtgtc aacctctaaa
    26701 atgcggaaga ctcaggaacg gatgcgtttg gcgcgtccgt atgccgaaaa agtgcgtatg
    26761 gtgatgagcc atcttgcgca aaccaatacc gatcatggta ttccgttact ggaatctcat
    26821 cgggagatta gacgtgtcgg ttttattttg attacgtctg ataagggttt gtgtggcggt
    26881 ttgaacgcca acgtgctgaa aaagtttttg gcacaagttc aagagtatcg gaatcaaggt
    26941 attgaagagg aaatcgtatg ccttggcagt aaaggtctga tggcgtgtca gagcattggt
    27001 ctgaatgtgg ttgccagtgc cgtaaatttg ggcgataccc caaaaatgga aatgctgctc
    27061 ggacctttga cagaactctt ccaacggtat gagaaacatg aaattgacag aatccatctg
    27121 gtgtattcgg gttttgtcaa taccatgcgt caagaaccga gaatggaagt attgctgcct
    27181 atcggtgaga acgtgattgg cgattcagct cccaaatcac cgttcagctg ggaataccgc
    27241 tacgaaccga ctgcacttgc agtgttggaa tatctggttc gccgctattt agagtctgtg
    27301 gtttatcagg cgttgagcga caatatggca tccgaacagg cagcccgcat ggttgccatg
    27361 aaagctgcaa cagacaatgc aggcaatgcc atcaaagagt tgcgtctggt atataacaaa
    27421 tcgcgtcaag ctgcgattac cacggaattg tcagaaattg tagcaggtgc ggcggctgtc
    27481 tgatgccgtc tgaatcctgt acagaaatta ggatacgata atgagccaag gcaaaatcgt
    27541 acaaattatc ggtgcggttg ttgacgtgga atttccacgc gacatgattc cgcgcgttta
    27601 cgacgctttg aaattagacg aaaacggtct gactttggaa gtccaacagc ttttgggcga
    27661 cggcgtagtc cgtaccattg cgatgggcag ctcggacggt ttgaaacgcg gcatgactgt
    27721 gagcaatacc ggtgcgccca ttactgtgcc ggtaggtaaa ggtacgttgg gacgcattgt
    27781 cgatgtattg ggaacgcctg ttgacgaagc aggtccgatt gataccgaca agagccgtgc
    27841 catccaccaa gccgccccta agtttgacga actgtcttcc acaaccgaat tgctcgaaac
    27901 gggcattaaa gtgattgact tgctgtgtcc gtttgccaaa ggcggtaaag taggtctgtt
    27961 cggcggtgcc ggtgtgggta aaaccgtgaa catgatggaa ttgatcaaca acatcgccaa
    28021 agcgcacagc ggcttgtccg tgttcgcagg cgtgggtgag cgtacccgcg aaggtaacga
    28081 cttctaccac gagatgaaag attccaacgt attggataaa gtggcaatgg tttacggtca
    28141 gatgaacgaa cctccgggca accgtttgcg tgtcgcattg accggtctga ccatggcgga
    28201 atacttccgt gatgaaaaag acgaaaacgg caaaggccgc gacgtattgt tcttcgtgga
    28261 caatatctac cgttacaccc tggccggtac cgaagtatct gcactgttgg gccgtatgcc
    28321 gtctgcagtg ggctaccaac cgacattggc agaagaaatg ggtcgtttgc aagagcgtat
    28381 tacctctacc caaaccggtt ccattacttc catccaagcc gtatatgtac ctgcggacga
    28441 cttgaccgac ccgtctccgg caacaacgtt cgcccacttg gacgcaaccg tcgtattgag
    28501 ccgcgatatt gcctctttgg gtatttaccc ggcagtcgat ccgcttgatt ctacttcgcg
    28561 ccaactggat ccgatggtat tgggtcaaga gcactacgac gtagcgcgcg gtgtgcagtc
    28621 caccctgcaa aaatacaaag aattgcgcga catcatcgcc atcttgggta tggacgaatt
    28681 gtctgacgaa gacaaactga ccgtaatgcg tgcgcgtaaa atccaacgct tcctgtccca
    28741 accgttccac gttgccgaag tgtttacagg ttctccgggc aaatatgtcg ccctgcgcga
    28801 taccattgcc ggcttcaaag ccatcttgaa cggcgaatac gatcatctgc ccgagcaggc
    28861 attctatatg gtcggcagca ttgaagaagc agtagagaaa gcgaaaacct taaactaagg
    28921 aggtcggcat gagcatcatg caagttgagg tggtcagtag cgagcaaaac atctattcgg
    28981 gcgaagccag ctttgtagtg gttccgaccg ttcaaggtga gcttggtatt tacccgcgac
    29041 acgagccgat tatgagtttg gtgcggccgg gggctttgcg tttgaccgtt ccgggcgagg
    29101 ataaagaggt tttggttgct gtttccggcg gtgttttgga agtacagcct gataaagtaa
    29161 ctgtcttggc ggatgttgcc gtccgcagtg cggagatgga tcaggcacgt gcggaagagg
    29221 cgaaaaaagc cgcagaagcg ggcatttccc aagctaaaga cgataaggct ttggcggaag
    29281 cacataaagc attggctgct gcgattgcac agctcaaaac tttggactat atccgttcgc
    29341 acaagaaata aacttgtttg gtttggaaaa gcacgaccgt ttggtcgtgc tttttatttt
    29401 tccaagtttt gaaaatatag tggattaaca aaaatcagga caaggcgaca aagccgcaga
    29461 caatacaaat agtacggaac cgattcactt ggtgcttcag caccttagag aatcgttctc
    29521 tttgagctaa ggcgaggcaa cgccgtactg gtttttgtta atccactata tcatgtatgc
    29581 cgtgttttca aatcagtctg tctttgagat aaacctatcc aaacagcaat ggaaacagta
    29641 aaataaaccc tttattttat cgaatcataa gccatgctta ccttccaaca aatcatcttc
    29701 aaactgcaaa cattctgggc agacaaaggc tgcaccgtca tccaaccctt cgacatggaa
    29761 gtcggcgcag gtacttccca ccccgccacc tgtctgcgcg cgctcggccc cgagccttgg
    29821 tttgccgcct acgtccaacc cagccgccgc cctaaagacg gccgctacgg cgacaatccc
    29881 aaccgcctgc aacactacta tcaattccaa gtcgccctca aacccgcccc cgccaatatc
    29941 caagacctct atcttgattc tctgcgcgaa ttgggcatcg accccaaagt ccacgacatc
    30001 cgttttgtcg aagacgactg ggaaaatccg actttgggcg cgtggggttt gggctgggaa
    30061 gtctggctca acggcatgga agtaacccag tttacctact tccaacaagt cggcggcatt
    30121 gactgcaccc ccgtactcgg cgaaattacc tacggcatcg aacgcttggc gatgtacctg
    30181 caaggcgtgg aaaacgtcta tgacctcgtt tgggcaaaaa cgctggacgg caataccgta
    30241 acctacggcg acgtgtacca ccaaaacgaa gtcgaacaat ccacctacaa cttcgaatac
    30301 agcgatgccg attggctgct gcgccagttc aacgactacg aagcgcaagc caaacgcctg
    30361 cttgccgttg aaggagcaag ccttgcgctg cctgcctacg agctggttct caaagcgggg
    30421 catacgttca acctcttgga cgcgcgcggc gcgatttccg taactgagcg ggcaacttat
    30481 attggacgta ttcgtgcatt gagccgcgcc gtggcgcaaa aatatgttga aagccgcgaa
    30541 aaactgggct tccctttaat gaaggcaaat gctgcctaag ggggcgacga ttttttacgt
    30601 tcgggcggca cgataaggca gccgtccgga agccattata ttttttagga aaatcatgcc
    30661 tgtctatttc cgcaccccaa gcaggaaacg cctgcacaaa atgaaccgcc gccagcataa
    30721 aaagctgcat ttgggcgagt ttcaaacatt gattttcggc gttcaaggca gtttgcacca
    30781 agctgcggac gatgccgaag cgtttggccg ctttatggac gagctgattg cgtatcttga
    30841 ggcaaatggc ttggaaatgg cgggcggggg cgattgcggc gatttttcgt gcctgtttca
    30901 aaaatccggt gcccgaagct tgggcgaaac cgagaaaaaa gccattatct cttggctggc
    30961 aggccgcgaa gatgtcgcca ctttgtacgc caccccgctg gttgatggtt tttacgggga
    31021 tgacggctgg gaaaattaca gcgaaaccta taaacaggca gattaaccaa attcaaagaa
    31081 agccgaatga tgacccaaac ccttttaatc gaactcctta ccgaagaact cccgcccaaa
    31141 gccctgaata atctgggcaa ccattttgcc gccgccgttg ccgaaggctt ggaaaaagcg
    31201 caactggttg acggtgcagc cgaatttacg gcttatgcct cgccgcgccg tttggcggtt
    31261 caagtcaaaa acgtcaaagc cgttcaggcc gaccaaaaaa tcgtgaaaaa aggccctgcc
    31321 gtggcgaatg ccgtaaaaga cggtgcgccg accaaggctt tggaaggttt cgcacgcggt
    31381 gcaggcgcga aaatcgaaga cttgaccatc gtccacgacg gcaagcagga tgtgtacgcc
    31441 tacgaatacg tccaaaccgg caagccgctg ggcggacttt tggaagacat tatcaatcaa
    31501 gtggttaaga aactgccgat tcccaaagtg atgcgttggg gcagcagcac atttaccttc
    31561 gtgcgccctg ttcacgggct gattgtgctg cacggcggcg acatcgtaaa cgtcagcgtt
    31621 ttgggtctgc aaagcagcaa tcaaaccttg ggacaccgct tcttgtccaa cggcgaaatc
    31681 accattgaaa acgccgacag ctacgccgaa caaatgcgcg ggcaaggcaa agtcgtcgct
    31741 tcgtttgccg agcgcaaagc cgcgattcag acggcattgg aagggcaggc gcgcagtctg
    31801 aacgccgccg tcgccgccga tgaagcattg ttggacgaag taaccgcgct ggtcgaatgg
    31861 cctgttgttt tggaagccgg ttttgaggaa catttcctcg ccgtgccgca ggaatgcttg
    31921 attttaacga tgcagcaaaa ccaaaaatac tttccgctgc tcgaccaaaa cggcaagctg
    31981 atgaaccgct tcctgctggt ttccaacctg caaaccgaag acccgtcgca catcatccga
    32041 ggcaacgaac gcgttttgcg cgcgcgcctg tctgatgccg agttcttcta caaacaagac
    32101 caaaaagcga ctttggaaag ccgcctgccg aaattgtcca gcgtggtgta ccacaacaaa
    32161 atcggctcgc aagccgaacg catcgaacgc ctgcaaagca ttgccgccca catcgccaaa
    32221 gcattgggcg cggatgccgc cgctgccgaa cgcgccgcgc gtcttgccaa agccgacttg
    32281 gtaaccgaaa tggtcggcga gtttcccgaa ctgcaaggca cgatgggcaa atactatgcc
    32341 cgcttggacg gcgaaaccga agaaatcgcc gaagccgtcg agcaacatta ccaaccgcgt
    32401 tttgccggcg acaagctgcc cgaaagcaaa attgccactg ccgttgcact tgccgacaag
    32461 ctggaaaccc tggtcggcat ttggggcatc ggtctgattc cgaccggcga caaagacccc
    32521 tacgccctgc gccgcgctgc cttgggtatt ttgcgtatgc tgatgcagta tggtttggac
    32581 gtgaacgaac tgattcagac ggcattcgac agcttcccca aaggtttgct caacgaaaaa
    32641 acgccgtctg aaaccgccga ctttatgcag gcgcgtttgg ccgtgttgct gcaaaacgat
    32701 tatccgcaag acatcgttgc cgccgtattg gcgaaacagc cgcgccgttt ggacgatttg
    32761 accgccaaac tgcaagccgt tgccgcgttc aaacagctgc ccgaagccgc cgcgctcgcc
    32821 gccgccaaca aacgcgtgca aaacctgctg aaaaaagccg atgccgagtt gggcgtggtt
    32881 aacgaaagcc tgttgcaaca ggacgaagaa aaagccctct ttgccgctgc gcaaggcttg
    32941 cagccgaaaa tcgccgccgc tgttgccgaa ggcaatttcc aaaccgcctt gtccgaactg
    33001 gcttccgtca aaccgcaagt cgatgcattc tttgacggcg tgatggtaat ggcggaagat
    33061 gccgccgtaa aacaaaaccg cctgaacctg ctgaaccgct tggcagagca aatgaacgcg
    33121 gtagccgaca tcgcgctttt gggcgagtaa ccgttgtaca gtccaaatgc cgtctgaagc
    33181 cttcagacgg catcgtgcct atcgggagaa taaattgcag cctttagtca gcgtattgat
    33241 ttgcgcctac aacgtagaaa aatatttcgc ccaatcatta gccaccgtcg tgaatcagac
    33301 ttggcgcaac ttggagattt tgattgtcga tgacggctcg acagacggta cgcttgccat
    33361 tgccaaggat tttcaaaagc gggacagccg tatcaaaatc cttgcacaag ctcaaaattc
    33421 cggcctgatt ccctctttaa acatcgggct ggacgaattg gcaaagtcag gaatggggga
    33481 atatattgca cgcaccgatg ccgacgatat tgccgccccc cgactggatt gagaaaatcg
    33541 tgggcgagat ggaaaaagac cgcagcatca tcgcgatggg cgcgtggctg gaagttttgt
    33601 cggaagaaaa ggacggcaac cggctggcgc ggcatcacag gcacggcaaa atttggaaaa
    33661 agccgacccg gcacgaagat attgccgact ttttcccttt cggcaacccc atacacaaca
    33721 acacgatgat tatgcggcgc agcgtcattg acggcggatt gcgttacaac accgagcggg
    33781 attgggcgga agattaccaa ttttggtacg atgtcagcaa attgggcagg ctggcttatt
    33841 atcccgaagc cttggtcaaa taccgccttc acgccaatca ggtttcatcc aaatacagca
    33901 tccgccaaca cgaaatcgcg caaggcatcc aaaaaaccgc cagaaacgat tttttgcagt
    33961 ctatgggttt taaaacccgg ttcgacagcc ttgaataccg ccaaataaaa gcagtagcgt
    34021 atgaattgct ggagaaacat ttgccggaag aagattttga acgcgcccgc cggtttttgt
    34081 accaatgctt caaacggacg gacacgccgc ccgccggcgc gtggctggat ttcgcggcag
    34141 acggcaagat gaggcggctg tttaccatga ggcaatactt cggcattttg caccggctga
    34201 ttaaaaaccg ccggcaggcg cggtcggatt cggcagggaa agaacaggag atttaatgca
    34261 aaaccacgtt atcagcttag cttccgccgc agaacgcagg gcgcacatta ccgatacctt
    34321 cggcgtgcgc ggcatcccgt ttcagttttt cgacgcactg atgccgtctg aaaggctgga
    34381 acaggcaatg gcggaactcg tacccggttt gtcggcgcac ccctatttga gcggagtgga
    34441 aaaagcctgc tttatgagcc acgccgtatt gtggaaacag gcattggacg aaggtctgcc
    34501 gtatatcgcc gtatttgaag atgatgtctt gctcggcgaa ggcgcggaga aattccttgc
    34561 cgaagacgct tggctgcaag aacgctttga ccccgattcc gcctttatcg tccgcttgga
    34621 aacgatgttt atgcacgtcc tgacctcgcc ctccggcgtg gcggactact gcgggcgcgc
    34681 cttcccgctg ttggaaagcg aacactgggg gacggcgggc tatatcattt cccgaaaagc
    34741 gatgcggttt ttcctggaca ggtttgccgc cctgccgccc gaagggctgc accccgtcga
    34801 ttggatgatg ttcagcgatt ttttcgacag ggaaggaatg ccggtttgcc agctcgatcc
    34861 cgccttgtgc gcccaagagc tgcattatgc caagtttcac gaccaaaaca gcgcattggg
    34921 cagcctgatc gaacacgacc gcctcctgaa ccgcaaacag caaaggcgcg attcccccgc
    34981 caacacattc aaacaccgcc tgatccgcgc cttgaccaaa atcagcaggg aaagggaaaa
    35041 acgtcggcaa aggcgcgaac agttaatcgg caagatcatt gtgcctttcc aataaaagga
    35101 gaaaagatgg acatcgtatt tgcggcagac ggcaggatgc ggcggctgtt taccttgagg
    35161 caatacttcg gcattttgca ccgattgctg aaaaaccgtt gaaaaacgcc gctttatcca
    35221 acagacaaaa aacaggataa attatgcaaa accacgttat cagcttggct tccgctgcag
    35281 aacgcagggc gcacattgcc gataccttcg gcaggcacga cattccgttt cagtttttcg
    35341 acgcactgat gccgtctgaa gcactggagc aggcaatggc ggaactcgta cccggtttgt
    35401 cggcgcaccc ctatttgagc ggagtggaaa aagcctgctt tatgagccac gccgtattgt
    35461 ggaagcaggc attggatgaa ggtctgccgt atatcgccgt atttgaggac gacgttttac
    35521 tcggcgaagg tgcggagaaa ttccttgccg aagacgcttg gctgcaagag cgttttgaca
    35581 aggattccgc ctttatcgtc cgcttggaaa cgatgtttat gcacgtcctg acctcgccct
    35641 ccggcgtggc ggactacggc gggcgcgcct ttccgctgtt ggaaagcgaa cattttggta
    35701 tggcgggcta tattatttcc cgaaaggcga tgcgtttctt tttggacagg tttgccgttt
    35761 tgccgtctga acgcctgaag gcggtggatt ggatgctctt cagcagtttt ctcgacaaag
    35821 gggggatgac ggtttgccaa ctgacccccg ccttgtgcgt ccaaagcgaa accctgccca
    35881 gccaactgaa aaacggcagg caggaatcgt accgcaaccg ccgctccccg aaggttttgt
    35941 tgaaacgcgc tttgggaaaa atcggcaggg aaatcgaaag ggcgagggaa cggaaacgtc
    36001 agaaaaaatt ggaaaaacac ctcgggcggc acgttgtccc gtttgaatag gttttggcct
    36061 ttccttattt ggcgggtgtg cgctgtccct aaatttatgt cggggaatcc ggtttgttcg
    36121 gtttcacttg ttttcgggta agccctaatt cgtgattccc gagaaaacag aaagccaaaa
    36181 acagaaacct aaaatctcgt cattctcgcg caggcgggaa tctaggtctt taaacttcgg
    36241 ttttttccga taaattcttg ccgcattaaa attctagatt cctgcctgcg cgggaatgac
    36301 ggcggggggg atttctgttt ttccgacaaa ttcctaaaac ttaaaattcc gttattcccg
    36361 cgagaacaga aaaccaaaaa cagaaacctg aaaacagaaa cctaaaattc gtcattcccg
    36421 cgcaggcggg aatccagtcc gttgaggttc agtcatttcc gataaatcac cgcaacatta
    36481 aatttccaga ttcccgcttt tgcgagaata acggcggggg gatttctgtt tttccgacaa
    36541 attcctaaaa cttaaaattc cgttattccc acgagaacag aaaaccaaaa acagaaacct
    36601 aaaatctcgt cattcccgcg caggcgggaa tccagtccgt tgagtttcag tcatttccga
    36661 taaatcaccg caacgttaaa tttctagatt cccgcttttg cgggaatgac ggcggggggg
    36721 atttctgttt ttccgacaaa ttcctaaaac ttaaaattcc gttatt
//
LOCUS       DAWFLT010000012        35134 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWFLT010000012 DAWFLT010000000
VERSION     DAWFLT010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35134
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.012"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(700..1284)
                     /gene="rnhB"
                     /locus_tag="WGR74_000583"
     CDS             complement(700..1284)
                     /gene="rnhB"
                     /locus_tag="WGR74_000583"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ0905064.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAAAWHVAAATPEEIASLNILHATMLAMKRAVDGLAVRPERIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDTEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(1352..3247)
                     /gene="mnmG"
                     /locus_tag="WGR74_000584"
     CDS             complement(1352..3247)
                     /gene="mnmG"
                     /locus_tag="WGR74_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ0905065.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRIFNEKREAVEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDDIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAR"
     gene            complement(3572..4999)
                     /locus_tag="WGR74_000585"
     CDS             complement(3572..4999)
                     /locus_tag="WGR74_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0905066.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            5159..6148
                     /gene="pdxA"
                     /locus_tag="WGR74_000586"
     CDS             5159..6148
                     /gene="pdxA"
                     /locus_tag="WGR74_000586"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ0905067.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPTAEAVEAGKLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKDLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGESGHL
                     GHEETDTIIPALKNLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            complement(7032..9791)
                     /locus_tag="WGR74_000587"
     CDS             complement(7032..9791)
                     /locus_tag="WGR74_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ0905068.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFRDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAELDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENSKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAGRADEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RGQTAPETATVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSETATFTAAAE
                     QARETAQTSGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
     gene            10285..11277
                     /locus_tag="WGR74_000588"
     CDS             10285..11277
                     /locus_tag="WGR74_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0905069.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPAREFDIVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEARYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPPDLAQFAVMLENGTKM"
     gene            11325..12497
                     /gene="lpxB"
                     /locus_tag="WGR74_000589"
     CDS             11325..12497
                     /gene="lpxB"
                     /locus_tag="WGR74_000589"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ0905070.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     VGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRSGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARQTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAACFLLPAATEATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQHDAVPEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            12505..12751
                     /locus_tag="WGR74_000590"
                     /pseudo
     CDS             12505..12751
                     /locus_tag="WGR74_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12753..13163
                     /locus_tag="WGR74_000591"
     CDS             12753..13163
                     /locus_tag="WGR74_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905071.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPTPNYTDWVKIKFKQFSYLKFVYGYATKSQDINIDNTLELGELKQDDEILDYGGA
                     LELIGGRYDFPTAFRIDIVCREIELEFLDQESFN"
     gene            complement(13277..14086)
                     /gene="dapB"
                     /locus_tag="WGR74_000592"
     CDS             complement(13277..14086)
                     /gene="dapB"
                     /locus_tag="WGR74_000592"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ0905072.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKASSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(14096..14473)
                     /locus_tag="WGR74_000593"
     CDS             complement(14096..14473)
                     /locus_tag="WGR74_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0905073.1"
                     /translation="MNKTLILALSALLGLAACSAERASLFPSYKLKVIQGNEIDPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKDRSNLTVYFENGVLV
                     RTEGNALQNAAEALRVKQNADKQ"
     gene            14692..15126
                     /gene="fur"
                     /locus_tag="WGR74_000594"
     CDS             14692..15126
                     /gene="fur"
                     /locus_tag="WGR74_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ0905074.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHSEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            15196..15921
                     /locus_tag="WGR74_000595"
     CDS             15196..15921
                     /locus_tag="WGR74_000595"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ0905075.1"
                     /translation="MRIPLLAPDNYAFPDPTYALARCDGLVGVSGDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDESGETRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(16073..17104)
                     /gene="gap"
                     /locus_tag="WGR74_000596"
     CDS             complement(16073..17104)
                     /gene="gap"
                     /locus_tag="WGR74_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0905076.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSILTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKDKLDGLAIRVPTVNVSLVDLNFQAARDTTIEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(17305..18144)
                     /locus_tag="WGR74_000597"
     CDS             complement(17305..18144)
                     /locus_tag="WGR74_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ0905077.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            18293..20269
                     /locus_tag="WGR74_000598"
     CDS             18293..20269
                     /locus_tag="WGR74_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ0905078.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVAKNA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            20339..20414
                     /locus_tag="WGR74_000599"
     tRNA            20339..20414
                     /locus_tag="WGR74_000599"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20372..20374,aa:Val,seq:tac)
     gene            20447..20523
                     /locus_tag="WGR74_000600"
     tRNA            20447..20523
                     /locus_tag="WGR74_000600"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20481..20483,aa:Asp,seq:gtc)
     gene            20943..21041
                     /locus_tag="WGR74_000601"
     CDS             20943..21041
                     /locus_tag="WGR74_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ0905079.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(21112..22497)
                     /locus_tag="WGR74_000602"
     CDS             complement(21112..22497)
                     /locus_tag="WGR74_000602"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEZ0905080.1"
                     /translation="MVSIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLVGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNGLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(22596..24986)
                     /gene="gyrB"
                     /locus_tag="WGR74_000603"
     CDS             complement(22596..24986)
                     /gene="gyrB"
                     /locus_tag="WGR74_000603"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ0905081.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLNV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDSRTIEGEELANTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVVVSYLEPKFLNSKAYQTL
                     TQTAAALKGMVGKGAKLYKGDNEYDIDSFEGALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            25256..26128
                     /locus_tag="WGR74_000604"
     CDS             25256..26128
                     /locus_tag="WGR74_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0905082.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNHPADSETL"
     gene            26216..28252
                     /locus_tag="WGR74_000605"
     CDS             26216..28252
                     /locus_tag="WGR74_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ0905083.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLGLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGKTIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
     gene            complement(28528..29094)
                     /locus_tag="WGR74_000606"
     CDS             complement(28528..29094)
                     /locus_tag="WGR74_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ0905084.1"
                     /translation="MSLYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMHEGLSGEAEDARENKRES
                     WWLTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVLVAVGLTVGRALGVLF
                     GRRAEFAGGLVLIAIGTWTLLSHLGLIG"
     gene            complement(29570..30070)
                     /locus_tag="WGR74_000607"
     CDS             complement(29570..30070)
                     /locus_tag="WGR74_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0905085.1"
                     /translation="MSNYRRDFTRGAHYFFTLALQDRTKDYLIRHIDRLRLAYRETKT
                     RYPFETAAICILPDHLHFIMRLPEGDHDYPIRIAYLKTRFSQLIPKECRAPNGSKTKK
                     REAGIWQRRFWEHLIRDEQDLNNHRDYIYFNPVKHGYAASVKEWRYSSFFKDVEKGIY
                     PLDWGG"
     gene            complement(30527..30955)
                     /locus_tag="WGR74_000608"
     CDS             complement(30527..30955)
                     /locus_tag="WGR74_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0905086.1"
                     /translation="MKAIQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQMSEAL
                     TLAEGQKTAVVEYYSDKGTFPKNNASAGIASASEITGKYVAKVEVSGATITSTMKSQG
                     VNKDISGATLVLEGKQNSGSFEWTCKAGSVKAKFLPSICR"
     gene            complement(31949..32129)
                     /locus_tag="WGR74_000609"
                     /pseudo
     CDS             complement(31949..32129)
                     /locus_tag="WGR74_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220242.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32298..33812)
                     /gene="katA"
                     /locus_tag="WGR74_000610"
     CDS             complement(32298..33812)
                     /gene="katA"
                     /locus_tag="WGR74_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ0905087.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            34153..35004
                     /locus_tag="WGR74_000611"
     CDS             34153..35004
                     /locus_tag="WGR74_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEZ0905088.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QESACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALKKFDPAGVAA
                     ADLNESLILQIERSGECAAKPSALHIVRNALDSIDGNRSQTLARIKKRLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESFEIPAA
                     HKRKTAE"
BASE COUNT         7262 a         8934 c        10376 g         8562 t
ORIGIN      
        1 cattcccgca aaaacaaaaa aaatcaaaaa cacaaaactg aaatatcgtc attcccgcgc
       61 aggcgggaat ctaggtctgt cggtacggaa acttatcggg aaaaacggtt tttccaaacc
      121 tgagactccg gattcctgtt ttcgcgggaa tccggttttt tgagtttcag tcatttttga
      181 taaattcttg cagctttgag tttctagatt cccgcttttg cgggaatgac gcggaaaagt
      241 tgctgtgatt tcggataaat tttcgtcacg cttaatttct gttttatccg ataaatgcct
      301 gcaatctaaa atttcgtcat tcccgcgaag gcgggaatct aggtctgtcg gtacggaaac
      361 ttatcgggta aaacggtttt gccagccctg agactccgga ttcctgtttt cgtaggaatc
      421 cggttttttg agcttcagtc atttttgata aattcttgca gctttgagtt tctagattcc
      481 cgcttttgcg ggaatggcgg tttggaagtt acctgaaatt caaaaaaaaa aaaaacggaa
      541 accggacgga ttggattccc gcctgcgcgg gaatgacgga ttttaggttt ttttttttga
      601 ttttctattt ttcgcaggaa tgacggtttg ggttctttct ctttgaagtt gcgatgctga
      661 aaatgccgtc tgaagttcag acggcatttt tgtgccggtt taaaacaagg cctgctgcgc
      721 gagcaggttt ctgacggggg cgaagtctcg gcggtgttcg ggcagcacgc cgtattgttt
      781 gagggcttcc agatgctgct tcgtgccgta acctttgtgt ttgtcgaaac cgtattgggg
      841 atggcgttgc gccagtgcgt acatttctgt atcgcgtgcg gtctttgcca aaacggatgc
      901 ggcggagatt tcgatgattt tgctgtcgcc tttgacgacg gcttcggcag ggatgttcaa
      961 atgttcggga atgcggttgc cgtcgatgaa tattctttcg ggacgcacag ccaaaccgtc
     1021 aacggcgcgt ttcatcgcga gcatggtggc gtgcaggatg ttgaggctgg cgatttcttc
     1081 gggcgtggcg gcggcgacgt gccacgcggc cgcctgattt tttatcattt cggcaagcgc
     1141 gtcgcgtttt ttctcgctga gttttttgga gtcggtcagt ccgggcaggt cgaatgtttc
     1201 cggaaggatg acggcggcgg caaacacgct gccgactaaa ggtccgcgtc ctgcctcgtc
     1261 cacgccggcg gtcagtatgt gcatgatgtt tcctgtcggg atggtacgat gccgtctgaa
     1321 aagggtttca gacggcatcg cgccgatgtg tttatctcgc gtctttaaat ccgcgcttca
     1381 aatgcaccat cagcaatgcc actgccgcag gggttacgcc ggaaatgcgg cttgcttgtc
     1441 cgacggtttc gggtttgtgc tgattgagct tttgctgcac ttctgccgac aagcctttga
     1501 ctttgccgta atcgatatcg tcgggcagct ttaaggtttc gatgtcgcgg cggctgtcga
     1561 tttcttcgtt ttggcggtcg atatagcctt ggtatttgac ttggatttcg acttgttcga
     1621 tgacttcggc ggagaggttt tcagacggca tcgcgccttc gagcgtcatc agcgcggcgt
     1681 agtcgaggtt cgggcggcgt aacaaatcgt gcaggttggc ctcgcggctg agtttttgtc
     1741 cgaacacgcg gatttgttcg tcttcggcga gtttttgcgg tgtgtaccac gttgttttca
     1801 agcgttggat ttcgcgttcg acggcttcgc gtttttcgtt gaaaatgcgc cattgcgctt
     1861 cacccaccaa gccgattttg tagccgtctt cggtcaggcg catgtcggcg ttgtcttccc
     1921 tgagttgcag gcggtattcg gcgcggctgg tgaacatccg gtaaggttcg ttcacgcctt
     1981 tggtgatgag gtcgtccacc aatacgccga gataggcttg ttcgcggcgc agcaggagcg
     2041 gatcttgtcc gcggacgtat tgcacggcgt tcgcgcctgc gagcaggcct tgcgcggcgg
     2101 cttcttcgta gcctgtcgtg ccgttgattt gcccggcgaa aaacagtccc tcaatggttt
     2161 tggtttcgag gcttgctttg aggttgcgcg gatcgaagta gtcgtattcg atggcgtagc
     2221 cggggcgcag gatatgggcg ttttccaggc ctttcatgct gcgaacgagc gcgatttgaa
     2281 tgtcgaaggg caggctggtg gagataccgt tggggtagta ttcgtgcgtg gtcaggcctt
     2341 cgggctcgag gaaaatctgg tggctgtctt tatcggcgaa gcggttgatt ttgtcttcga
     2401 tagacggaca ataacgcggg cccacgcctt cgattttgcc ggtgaacatc gggctgcggt
     2461 cgaagcctga gcggatgatg tcgtgggttt gcgtgttggt atgcgtaatc cagcaggaca
     2521 cttggcgcgg gtgcatttcg gcgttgccgc gcacggacat gacgggaacg ggtgtgtcgc
     2581 cgggctgttc ggtcagttgg gaaaagtcaa tcgtgcgtcc gtcgatgcgc ggcggcgtgc
     2641 cggttttcag acggccttgc ggcagcttca attcgcgcaa acgtccgccc agcgatttgg
     2701 cggcggggtc gccggcgcgt ccgccctcgt agttttccaa accgatgtgg attttgccgg
     2761 acaaaaacgt gcctgcggtc aacaccacgg cgcgtgcttt aaactccacg cccatcgcgg
     2821 tgattacgcc tgaaatgcgg tcgccatcga gcgttacgtc ttcgacggct tgttggaaaa
     2881 ggtcgaggtt ttcttggttt tccaacattt cgcggatggc ggctttatat aggatacgat
     2941 ccgcctgcgc gcgcgtggcc cgtactgccg cgcctttgct ggcgttcaga cggcggaact
     3001 ggatgccgga tttgtcggtt gccaacgcca tcgcgccgcc gagcgcgtcg agttcgcgca
     3061 ccaaatgccc tttgccgatg ccgccgatgg aagggttgca cgacatttgt ccgagcgttt
     3121 caatattgtg tgtgagcaaa agcgtctgcg cgcccatacg ggcggcggcg agcgcggctt
     3181 ctgtgccggc gtgtccgccg ccgacgacga taacgtcgta ggttttgggg taaatcatgt
     3241 gggtcatgtg tgtattgcct gtcggtgttt cagacggcat ttatagtgga ttaacaaaaa
     3301 ccagtacagc gttgcctcgc cttagctcaa agagaacgat tctctaaggt gctgaagtac
     3361 caagtgaatc ggtttcgtac tgcttgtact gtctgcggct tcgtcgcctt gtcctgattt
     3421 ttgttaaacc actatattca atatgccgtc tgaaaaacga aatggattca aaagtaaagg
     3481 gttgggattg tacgcttgtt cgccctgttt ttacagtgtg cggaaaggga aaagccgctt
     3541 cgcggggaag cggctccggt aagggcggga tttaccaaac gtcggatttg atacggcgtt
     3601 tcaggcccgg atgttcggaa agtttgaact cggggtcttt gcccattttc agcttggcgg
     3661 tgtaatcgcg cagcagcata aacgccaagg gcgagagcag caggatggcg acaaggttga
     3721 tccacgccat aatgcccatc gccatatccg ccatatccca gaccaaaggc acattggcaa
     3781 ccgcgccgaa atagacccac gccaaaacca gcatacggaa aacggcggta atcagccaat
     3841 ggcttttgat gaattggacg ttggactcgg cataggcata gttgccgata acggtggaaa
     3901 aggcaaacat aaacaggatg acggcgagga agcccgcgcc ccattgcccc acttggctga
     3961 caatcgccgc ctgcgtcaac gccgcaccgc tcaaatcgcc gtaaggctgt tggtaaatca
     4021 agatgatgaa ggcggtgcaa gaacaaacga tgatggtatc gacaaacacg cccagcattt
     4081 gaatcatacc ttgcgaaaca gggtgtttca cttcggcggc ggcggcggcg ttcggcgcgg
     4141 aacccatacc cgcctcgttg gaatacaggc cgcgtttgat gcccatcatc atcgtttgcg
     4201 aaatcagacc gccgagtaag ccgcctgctg ccgcatcgaa tttgaacgcg cccgaaaaaa
     4261 tctgaccgaa cacgtccgga atcatcggaa tattggtcaa aatgatgaaa agcgcgataa
     4321 agaggtacaa aaccgccatc aggggcacga cgatttccgc cgctttagat atgcgcctga
     4381 tgccgccgaa gataatcggc gcggttaaaa tcaccagggc gacgccgaca taatgaggct
     4441 cccaacccca tgccgctttg acggtatcgg cgatggtatt ggtctgaacc gcttcaaaca
     4501 caaagccgaa acagaaaatc aggctcaggg cgaacaacac gcccagccat ttctgcccca
     4561 gcccttgagt gatgtagtag gcagggccgc cccggaaatg gtggttgtcg tagtcgcgga
     4621 ctttaaagag ctgcgccagc gaagattcga caaacgccga actcataccg attaaggcgg
     4681 ttacccacat ccaaaacacc gcgcccggcc cgccgacttt gatggcgatt gccacgcccg
     4741 cgatattgcc cacacccacg cggctggcaa ggccggttac aaatgcctga aacggcgtga
     4801 tgccgtgagg gtcgtccccc tgtttgcggc cgccgagcat ttctttgatg ctgcgcccga
     4861 acaggcggaa ttggacaaag cccgtggtta cggtgaagaa aagccccgta cccaaaagca
     4921 tataaaccaa gtatgaccac atcggatcgt tgatggcgcc gacccagccg tgcagccatt
     4981 cggtaaagtt ctcgttcata tcgcttcctt aaagttgaaa cccgcacata ttggcggtat
     5041 gcaagcaggg tttaaatttt gtaaacgccc attctagcag attgtcaaca aaatcagaaa
     5101 aatttacatc gccgcgcggc tgcggcgtta gaatcgcatt ttgtttggag caaacacgat
     5161 gaaacagcct gtttttgccg ttacttccgg cgagcctgcc ggcatcggcc ctgatatttg
     5221 tttggacttg gcgtttgcac gcctgccctg ccgctgcgcg gtattgggcg acaaacacct
     5281 gttgcgcgcg cgcgccgaag ccttgggcaa aagcgtcgtc ttgcgcgact tcgatccgga
     5341 atcaggcggc gcggcatacg gcgagctgga agtgctgcac attccgaccg ccgaagcggt
     5401 tgaggcgggc aaactcaatc ccgccaacgc cgcctatgtg ctgcaacttt tggacaccgc
     5461 gctcgcaggc atttcagacg gcattttcga cggcatcgtt accgcgccgc tgcacaaagg
     5521 catcatcaac gacgcgcgcg caagcacagg ttttttcagc gggcacaccg aatatctggc
     5581 ggaaaaaagc ggcacggggc aggttgtgat gatgcttgcc ggcaaagacc tgcgcgtcgc
     5641 cctcgtaacg acccacctgc cgctgaaaga cgttgccgcc gccatcacgc aaccgctgat
     5701 tgaaagcgtc gcacgcattt tgcatcacga cttaaaacac aaattcggca tcaaaaatcc
     5761 caaaatccta gtcgccggac tcaaccccca cgccggcgag agcggacacc tcggacacga
     5821 agaaaccgac accattatcc ccgcattgaa aaacctgcgc cgcgaaggga taaacctcgc
     5881 cggcccgtat ccggcggata ccttgttcca accgtttatg ctcgaaggtg cggatgccgt
     5941 attggcgatg taccacgacc aagggctgcc cgtgttgaaa taccacagct tcggacaggg
     6001 cgtgaacatc acactggggc tgccctttat ccgcacctcc gtcgatcacg gcaccgcgct
     6061 cgatttggcg gcaaccggca gggcggattc cggcagcctg ataactgccg tggagaccgc
     6121 cgtcgagatg gcgcgcggca gcctttaaag atgataaaag acccgtcatt tccgcgcagg
     6181 cgggaatccg gtctgttcgg tttcagttgt ttttgggttt cgggtaattt ccaaatcgtc
     6241 attcccgcgc aggcgggaat ccagaccttc gggcgacggc aatattcaaa gattatctga
     6301 aagtttgagg ttctagattc ccgttttcac gggaatgacg aaaggttgcg ggaatccggc
     6361 ttgttcggtt tcggtttttt ttgaggtttc gggcaacttc taaaccgtca ttcccgcgca
     6421 ggcgggaatc caggcctttg ggcgacggca atattcaaag attatctgaa agtttagagg
     6481 ttctagattc ccgttttcac gggaatgacg gaatgttgtg ggaatccaga cctttgggcg
     6541 gcggcaatat tcaaagatta tctgaaagtt tgaggttcta gattcccgtt ttcacgggaa
     6601 tgacggaatg ttgcgggaat ccagaccttc gggcggcggc aatattcaaa gattatctga
     6661 aagtttagag gttctagatt cccgttttca cgggaatgac ggaatgttgt gggaatccag
     6721 acctttgggc ggcggcaata ttcaaagatt atccgaaagt ttgaggttct agattcccgt
     6781 tttcacggga atgacgaaag gttgtgggaa tctagacctt tgggcggcgg caatattcaa
     6841 aaattatctg aaagtttaga ggttctagat tcccgttttc acgggaatga cggaatgttg
     6901 cgggaatcat gggaatggag gaatgtttcg gtaatcacgg gaatggcgaa gtttcagacg
     6961 gcattgcagg tatccgaacc catgtaaaaa agaggttctg cggaacagaa cctctttttg
     7021 ccgccgtcgg ttcagccttg ccgggtttcg acttggatca tttcttcggc agggacggtt
     7081 gcgacttcag acggcttggg ctgttcggaa cggcgcaaac cgcgtccggc ttggacttcg
     7141 ggttgtgccg cccatgcctt caatgcggca gggtcggttt cgatcaggac gagtccgctg
     7201 gtttgtgcgg tttcccgtgc ctgttccgcc gcagccgtaa aggttgcggt ttcagacggc
     7261 atttcctgtg cttcggcttt cggtgtcgcg ccttcgggca ggatggcggc ggtggcacgg
     7321 cggatttttt ccgccgcatc ataaaccggt gcgtcgccgt ttgaaacggc gggagatgct
     7381 gtcggaagat ccctttctgc aaccggatcg gcaatgctga cggtaatcgg cgcgtttgcg
     7441 tcggtttcgc cgaaaacgtg cgcggcggcg gaacggactt tgtcggcggt gtcgtgaata
     7501 ttcaggtact gctcgatttt ggcggcagac ggaatattgc gttttttgcc gttttgacgg
     7561 cggtcgcgct gattgttgcg ctcgcggcgt tctttggcat ctcggctgtc gcgttcgcgg
     7621 cggttgcgtt cggatttggg cttgctgcct ttgtcttctg cggtatgcgg ttcggacggc
     7681 gtgttttccg ctgtctgaac ggttgtttcg gcaacggtgg cggtttccgg cgcggtttgg
     7741 ccgcgttcgc tgcggctgcc gttgcggcgg cgttttccgg tttgcacttc ggtttcggac
     7801 ggtgcggcat ctgcaacggt tgcggcaggc tgtatgttgc ggtcttggat ttccgcttcg
     7861 tcggcgcgtc cggcggcacg gtcgccgcgt tcgttgcggc ggcggttgcc gttgttgcgc
     7921 gtttcggctt tgtcggcacg cgcttcctgt ccggcagttt tgcctgccac ttcgcggact
     7981 tctactttgc tgccttcgcg tttgctgcgg cgcgggtttt ggcggcggtt gttggcgcgg
     8041 ctgccgctgc ggtttgccgt gctgcgtttt tcggaggttt cggcagcggg cgcggcttgg
     8101 gtttcgctgc cgccgaaaat gcgtttgagc caagctttga agctgtccca ccaggaggtt
     8161 tttttctcgg gggcggcagt cggggcgggg ctggtgtggc gcacgccttt gacggcgggt
     8221 tcgggacggg cggctttggc tttttcgccg ccgaacggtt tggcggattc gtcttcttca
     8281 ggctcggcga cgcgtttgta gctcggttcg ccgtcttctt ctacgtcgtc ggtgcggatg
     8341 cggttgattt cgtagtgcgg attttcgagg tggatgttcg gaatcaggac gacgttgaca
     8401 tccaaacgct cttccatcgc aaacagctcg gcgcgttttt cgttcagcag gaaggtggcg
     8461 acatcgacgg gcacttgtgc gcgcacttct ccggtgttgt ccttcatcgc ttcttcttga
     8521 atgatgcgta aaacgtgcag ggcggtggat tcgatgcccc gaatcacgcc ggtgccggcg
     8581 cagcgcggac aggcgacgtg gctgctttca cccaaagccg gtttcaggcg ttggcggctc
     8641 aattccaaaa gtccgaaacg ggagagtttg cccatctgca cgcgggcgcg gtcttttttg
     8701 agcgcgtcgc gtaggacgtt ttccacatca cgctggtgtt tggagttttc catgtcgatg
     8761 aagtcgatga cgaccaagcc gcccaagtcg cgcaggcgca tttgtcgggc gacttcttcg
     8821 gcggcttcca tattggtttt gaaggcggtg tcttcgatgt ccgcaccgcg tgtggcgcgt
     8881 gcggagttga catcgatgga gacgagggct tcggtgtggt cgatgacgat tgcgccgccg
     8941 gaaggcaggc tgacgctgcg agaaaacgcg ctttcgattt gatgttcgat ttggaagcgg
     9001 gaaaacagcg gcgtgtggtc ttcgtagagt ttcagacggc ctatattgcc cggcatgacg
     9061 tagctcatga actcggcaac ttggtcgtaa acttcttgat tgtccaccaa aatctcgccg
     9121 atgtcggggc ggaaatagtc gcggatggcg cggatgagca gcgagctttc cataaagagc
     9181 aggtaagggt cgtgatgcgc ttttcctgct tcttcaatcg cctgccagag ttgtttgagg
     9241 tagttcaagt cccattccaa ctcttccgcg ctgcggccga tgccggcggt acgggcgatg
     9301 atgctcatgc cgtttggaat gtcgagttcc gccatggcgg ctttcaactc ttgacgctct
     9361 tcgccttcga tacggcggga tacgccgccg ccgcgcgggt tgttcggcat caataccaga
     9421 tagcgtccgg cgaggctgat gaaggtggtc agtgccgcgc ctttgttgcc gcgctcgtct
     9481 ttttcgactt ggacgatgac ttccatacct tctttgagca cgtcctgaat acgcgccctg
     9541 ccgccttcgt agtcgcggaa atacgaacgc gatacttctt taaacggcaa gaagccgtgg
     9601 cggtcggttc cgtaatccac gaaacacgct tccagcgacg gctcgatgcg ggtaatgatg
     9661 cctttgtaga tattgccttt gcgctgttct ttgcccagcg tttcgatgtc caaatccagc
     9721 aggttttgtc cgtcgacgat ggcaacgcgt agctcttcgg cctgcgttgc gttgaataac
     9781 attcttttca tgatcacctc gtggacgggc ggcattcggg cgcgccggtc cggttcggca
     9841 ttccgtaagg ctgggttttc cgatgtcttc ggataaaacc ggtaatcagt ttttgagttg
     9901 aaaatccgca gggatgcacg ttccggagaa ccgtgtgcgg aagggtcgga tacagaaggc
     9961 tataaagatc gatgcggcgg tctgtctgcc gcgttccgaa cgctgcggtc ggaaaaatgg
    10021 aggccggctt cttcttgtta tcgtgatgcc tgtgttttgg gcggtttgcg tttgggactt
    10081 ggggcccggc tgccgtctta cttccgcgcc gaaacggcaa aatcaattca aacttgatta
    10141 cgttctgcgc ctgccggctg ggaacaggcg cagggaaaat gctttgcgga gtgcgttttt
    10201 aatataaaat tccgttttaa agtaaaccgt ttcagggggg cgcggcgggc gcgctttttg
    10261 ctgaaacgga tgttcggatt atagatgaaa acgcacgaaa taagcaaaga ttcggtcagc
    10321 ttgatagggg ttgccgaaca cgaggcgggt caacgccttg ataactatct gataaaaatc
    10381 ctcaagggtg tgcccaagag ccatatccac cgcattatcc gcgccggcga ggtgcggttg
    10441 aacaaaaaac gctgcaaacc cgacagccgt attgcggagg gggatacggt gcggattccg
    10501 cctgtgcgcg tggcggagaa ggaaatgccg tctgaaaggc gtgccgccgt gccggcgcgc
    10561 gaatttgaca ttgtttacga agacgacgcg ctgctggtga tcgataagcc ttccggcgtg
    10621 gcggtgcacg gcggcagcgg cgtgagtttc ggcgttatcg aacagttgcg ccgcgcccgt
    10681 ccggaggcgc gttatctcga actcgtccac cgtttggaca aggatacgag cggcttgttg
    10741 atggtggcga agaaacgcag cgcgctcgtc aaacttcacg aagccatccg taacgaccac
    10801 cccaaaaaaa tctaccttgc gctgggggtg ggcaaactgc cggacgacaa tttccatgtc
    10861 aaactgcccc tgttcaaata taccggcgca caaggcgaaa agatggtgcg cgtcagtgcg
    10921 gacgggcagt cggcgcatac ggtgttccgt gtgttaagcc gtttttcaga cggcattttg
    10981 cacggtgtcg ggctgtcgca cctgactttg gtgcgggcga cgttgaaaac ggggcgcacg
    11041 caccaaatcc gcgtccacct gcaatctcaa ggctgtccga ttgcgggcga cgaacgctac
    11101 ggcgattatc aggcgaaccg tcgtttgcag aagttgggtt tgaagcggat gtttttgcac
    11161 gcgtccgagc tgcacttgaa ccatccgctc acgggcgagc cgctggtgtt gaaggcggag
    11221 ctgccgccgg acttggcaca gtttgcggtg atgttggaaa acgggacgaa aatgtgaacc
    11281 ccgatgccgt ctgaagcctt cagacggcat cgggacgtga aagtatgtgg ggacagacga
    11341 atatggttga taaaaaaagc cctttgattg ccgtcagtgt cggcgaagcg tcgggcgacc
    11401 tgttgggggc gcacctgata cgcgccatcc gcaagcgttg tccgcaggcg cgttttgtcg
    11461 gtatcggcgg cgaactgatg aaggcggaag gtttcgagag cctttatgat caggagcggc
    11521 tggcggtgcg cggctttgtc gaagtggtca ggcggctgcc ggaaatttta cggatacgca
    11581 gggggctggt acgggatttg ctgtcgttga aacctgatgt ctttgtcggt atcgatgcgc
    11641 ccgattttaa tttgggtgtg gcggaaaagc tgaaacggtc ggggattccg accgtgcatt
    11701 atgtcagccc gtcggtgtgg gcgtggcggc gggaacgtgt gggcaaaatc gtgcatcagg
    11761 tcaaccgcgt gttgtgcctg ttcccgatgg agccgcagct ttatctcgat gcgggcggac
    11821 gtgcggagtt tgtcggtcat ccgatggcgc agcttatgcc cttggaagac gaccgtgaaa
    11881 cggcgcggca aactttgggc gtggatgccg gcatccccgt attcgccctg ctgcccggca
    11941 gccgcgtcag cgaaatcgac tatatggcgc cggtgttttt tcagacggca ttattgttgt
    12001 tggaacgcta tcctgccgca tgcttcctgc tgcctgccgc aacggaggcg acgaagcggc
    12061 gtttggcgga agttttgcag cggccggagt ttgccggatt gccgctgacg gtaatcgaca
    12121 gacagtctga aacggtgtgc agggcggcgg atgcggtgct ggtaacgagc ggtacggcaa
    12181 ctttggaggt ggcgctgtgc aagcgtccga tggtcatcag ctacaagatt tcgccgctga
    12241 cttatgctta tgtgaaacgc aaaatcaaag tgccgcatgt cggcctgccg aatatcctgt
    12301 tgggtaagga ggctgtgccg gagctgcttc aacatgatgc cgtaccggaa aaactggcgg
    12361 cggcgttggc ggactggtac gaacaccccg ataaggttgc cgcgctgcaa caggatttca
    12421 gggcgttgca cctgctgttg aaaaaagata cggcggattt ggccgcgcgc gcggttttgg
    12481 aagaggcggg atgttgagcg gttaatggat tatcttcttg aagcagcacg tattacaaaa
    12541 aaagggggga gaaattgtga ttaatggcac atcaaacaat aagtatttaa gaggaattcc
    12601 aaatgaagga gaactggctc gagtgggatt aaggttaaaa tataatgggc cgttaaagga
    12661 agagtttaaa caactgaaat ttcataagag tagtagaacc aacttggact caaaaaatct
    12721 taaactcatt gtttttgaaa aggtaaaata atatgacaac ttataccatt ccaaaaaaag
    12781 attatcaatt tctgtatata tatgagggca ctctattaaa ctatactttg aaaaacgatg
    12841 aattccatat catcgtccag aatgtggatt atccggactt tcctcaagag attcctacac
    12901 caaattatac agactgggta aaaattaaat tcaagcagtt cagctatctg aaatttgtct
    12961 atggatacgc tacaaagagc caagatataa atatcgataa tacattggaa cttggcgaat
    13021 taaagcagga tgatgaaatc ttggattatg gaggtgcgct ggaattgata ggcggtaggt
    13081 atgattttcc gactgctttt aggatagata tagtttgccg ggaaatagag ttagaatttt
    13141 tagatcagga gagttttaat taaacgagcc gtaacttgtc atgccgcgca agcaacttta
    13201 ttgatatgcc gatacggagc ctgttggcaa aatgccgtct gaacaatatc ttttcagacg
    13261 gcattttgta tgggggttaa cggttgttca gcccgagtac gtcctgcata tcgtacaaac
    13321 ccgttttgcc gttgacccaa actgcggcgc ggacggcacc ggcggcaaag gtcatgcggc
    13381 tgctggcctt gtgggtgatt tccacgcgct cgccgtcggt ggcgaagagg gcggtgtggt
    13441 cgccgacgat gtcgcctgcg cggacggtgg caaagccgat ggtcgacgga tcgcgcggac
    13501 cggtgtggcc ttcgcggccg taaacggcgc attgtttgag gtctctgccg agcgcgccgg
    13561 cgatgacttc gcccatgcgt aacgcggtgc cgctgggggc atcgactttg tggcggtggt
    13621 ggccttcaat gatttcgatg tcgtagcctt cgtttaatac gcgtgcgacg gtgtcgagga
    13681 tgtggaaggt gaggttgacg ccgacgctga agttggcggc gaaaacgatg cctgtttgtt
    13741 cggcggcagt gtggatagcg gctttgcccg catcgtcgaa gcctgttgtg ccgatgatga
    13801 tgttgacttg tttttcaacg catttttgca ggtgtttgag ggtgggctcg gggcgggtga
    13861 agtcgatgag tacgtcgctt tgtgcgagaa cggcgtcaac gtcgtctgaa atggcgatgc
    13921 cggttttgag tccgacggcg tagcctgcgt ccagtccgag ggcttctgag cctgagtgtt
    13981 cgagcgcgcc ggaaaggacg gtgtcgggat ggttgttgac ggcttcaacc aatacgcgtc
    14041 ccatacggcc gtttgcgccg gcgatggcga ttttgagcgg tgtcatgtgt gttccttatt
    14101 gtttgtctgc gttttgtttt acgcggaggg cttcggcggc attttgcagg gcgttgcctt
    14161 cggtgcggac gagcacgccg ttttcaaaat agacggtcag attgcttcgg tctttgatga
    14221 tgccgttgcg ggaggtgttg aaggtatagt cccagcggtc ggtatggaat gcgtcgcgca
    14281 gtatggggct gccgagcagg agcaggactt ggtctttggt cataccgggg cggagggcgg
    14341 caacggcgcg ggggtcgatt tcgttgccct gtatgacttt gagcttgtat gaggggaaca
    14401 gcgaggcgcg ttcggcactg cacgcggcaa ggccaaggag ggcggaaagg gcgaggatga
    14461 gggttttgtt cacggaaatg cctttctgtg caaatcggga tgggtagtgt aacactgctt
    14521 gaatatttta taaaagcgaa cgataatcat acgattaagc ggtatccgcc ctgtccgcgc
    14581 atcggccgcc ggtgcggttt tactattgca aactgctatg gtgcgatagt gggcaaacag
    14641 gccgaaattg cgtattataa cgtctattgt tttacagggg tattgaatat tatggaaaaa
    14701 ttcaacaata ttgcacaact gaaagacagc ggtctgaagg ttaccggccc gcgtttgaag
    14761 attttggatt tgttcgagac gcattcggaa gagcatttga gtgcggaaga tgtgtaccgc
    14821 attttgttgg aagagggtgt ggaaatcggt gtggcgacga tttaccgtgt gctgacccag
    14881 tttgagcagg cgggtattct gcaacgccat cattttgaaa cgggcaaggc ggtttatgag
    14941 ttggacaaag gcgaccacca tgaccacatc gtctgcgtga agtgcggcga ggtaacggaa
    15001 ttccacaatc ccgaaatcga agccctgcaa gacaaaatcg ctgaggaaaa cggctaccgc
    15061 atcgtcgatc acgcgcttta tatgtacggc gtgtgcagcg actgtcaggc caagggcaaa
    15121 cgttaaatcc ggacggtttg ttgttcagac ggcattcatg attttggatg ccgtctgtgt
    15181 ttttggagaa ctgccatgcg tattccgctg cttgcccctg acaattatgc ctttcccgat
    15241 cctacctatg ctttggcccg gtgcgacggg ctggtcggcg tgagcggcga tttggatgcg
    15301 gggcggctgc tcgaggcgta tcggaacggc gtgtttccgt ggttttctcg ggacggttgg
    15361 tttttttggt atgcggtcgg cccgcgtgcg gtgattgttc ccgaacgtct gcacgttccg
    15421 cgctcgctgg cgaaaacgct gcgcaacggc agctatcggg ttgcggtcaa cggctgtttt
    15481 gcggaagtgg tcgcgcattg tgcggcagcg gcgcgcccga atcaggacgg aacttggatt
    15541 gcgcccgagt ttcagacggc atatttgaag ctgcacgaaa tgggacacgc gcattcgttc
    15601 gagtgccatt atcccgatga aagcggtgaa acgaggttgg cgggcggctt ttacggcgtg
    15661 caaatcggca gggtgtttta cggcgaatcg atgttcgcat tacaaccgga tgcgtcgaaa
    15721 atcgcgtttg cctgcgccgt gccgtttttg gcggatttgg gtgtggaact gatagactgc
    15781 cagcaggaca cggaacatat gcgccgtttc ggttcggagc tgctgccgtt tgcggatttt
    15841 gccgaacgtc tgcggatgtt gaacgccgtg ccgttgaaag aggaaatcgg gcggcgcgaa
    15901 gtggcgtgca aggggctttg atggcggctt atgctccggt caggttcaaa tatggtggat
    15961 taaaattgga aatggcgaca gccgggcaaa atcactgcca aaaaatgaaa aatgccgtct
    16021 ggaacttgaa aacatcgggt ttcagacggc attttgtttg acggtttatt gcttatttga
    16081 gcgggcgcac ttcaagtccg aacatacggc gtgcggtgtt cagcatttgg cagctgaagc
    16141 cccattcgtt gtcataccat gcgaagactt taatcatgtt gccgtcaacg actttggtca
    16201 gtgttgcgtc gaagtggctg gcttcggtgg tgtggttgaa gtccatggaa accaagggca
    16261 gggtgttgta gcctaaaacg cctttgagcg cgcctgcttc cgaggcggct ttcatcagtg
    16321 cgttgatttc ttcgattgtc gtgtcgcgcg cggcttggaa gttcaaatct accaatgata
    16381 cgttgacggt cggcacgcgg atggcaagcc cgtcgagttt gtctttcaat tcgggcagta
    16441 ccagtccgac ggcttttgcc gcgccagttt tggtcggaat catgttttcc acgccgctgc
    16501 gggcgcggcg caggtctttg tggcgcacgt cggtaacggt ttggtcgttg gtcagcgcgt
    16561 ggatggtggt catcgcgcct ttgacgatgc cgacgctttc gctcaacact ttggcaaccg
    16621 gcgagaggca gttggtggtg caggaagcgt tggaaacgac ggtcatgtcg gcggtcagga
    16681 tgctgtcgtt cacgccgtac acgacggttg catcgacatc gtcgccgccc ggtgcggaaa
    16741 tgaggacttt tttcgcgccg ctttccagat gaactttggc tttttctttg ctggtgaacg
    16801 cgccggtgca ttccatgacc aaatcgacac cgagttcttt ccacggcagt tcggcagggt
    16861 tgcgggtcga gaagaagggg attttgtcgc cgttgacgat gaggttgccg ccgtcgtggg
    16921 atacgtcggc ttcaaagcgt ccgtgcacgg tgtcgaattt ggtgaggtgg gcgttggttt
    16981 caaggctgcc gctggcgttg acggcgacga tttggagttg gtcttgaatc tgataatcgt
    17041 agatggcgcg caaaacctgg cggccgatgc gtccgtagcc gttgatggcg actttgatgc
    17101 ccatggtttg ttcctttgtt gaggggttgg gtagattttc ggggcggatt atagcaaatt
    17161 tgtagtggcg tgtaattaat attttattga aaacggcgcg gccggaaggg tgggcggtaa
    17221 gatgcggacg gcacgggtgc ggcggacgga gagcttgata aaatgccgtc tgaaacggtt
    17281 ttcagacggc atatcaggga agggtcagga ggcggtattc tgtgcggctt cctgtttggc
    17341 tttgtattgt ttgagatatt cgagggcggc ggctttttcg ctgtcgctgc cgtatttcat
    17401 atcgcgttgg gcgcggcgca actcggcgcg ttcgcgggct tcggctatct gtttcgcctg
    17461 atagtctttg cggttgtcgg cggcggcgag gcggtcttgt tgggtttgcg cttttgccat
    17521 ggctttggcg atgaggtcgg cagggttaaa cgccggtttt ttcggtgtgt cgggtgtttg
    17581 cggacgcgcg ttgcggacgg cggcttcgcg ttcggcaagc atggccttgc gttcgtcggc
    17641 ttcgcgctgt ttgcgttcgt tgcgtttgag gtagcgcgca cgcgcgtgtt cggcggcggc
    17701 aaaacggctg tcggcggaca ggctgaagcg gcgcgcgcgg ggcaggacgg tgtcggcaac
    17761 gggctgcata tggatgcagt cgacggggca gggggcgacg cagagtccgc agccggtgca
    17821 ttcgtcggcg atgacggtgt gcataagttt gcccgcgccc ataatggcat cggcagggca
    17881 ggcgcggatg caggcggtgc agccgataca ggcggtttcg tctatccggg cgagtgcttt
    17941 ggcttgggtt ttggcaggtg cgacaagggg tttgccgagc agggcggaaa tgtcccgaat
    18001 gacggcttct ccgcccgggg cgcagaggtt gtacgcttcg cctgttgcga ctgcctgtgc
    18061 gtagggcagg cagccgtcgt agccgcattc gcggcattgg gtttggggaa gcaggcggtc
    18121 tatggcggcg gctgtggcgg tcatgtcggt gtgcggctca aaatcgaaag ggcgtatttt
    18181 agcagaattg tatgccgcgc ccgtttcgga tggtgcgcgg tgttttgtta taatacggcg
    18241 gcgtatgccg tttcagacgg catttttctg tattttcctg ttcggacggc ctatgaacga
    18301 attttcgctt gcccctattg tggttgtttt gctggtgtcg gtcattacgg tgatcctgtg
    18361 ccgcaagttc aacattccct ccatgctggg ctacctgctg gtgggctttt tggcggggcc
    18421 cggtatgctc agcctgattc cgaaaagcca tgcgacggat tatttgggcg aaatcgggat
    18481 tgtgttcctg atgttcagca tcggtttgga gttctcgctg cccaagttga gggcgatgag
    18541 gcggctggtg tttgggctgg gcggtttgca ggtcggcgtt acgatgctgt cggtaatggg
    18601 catactgatg ctgacgggcg tcccgttcaa ttgggcgttt gccgtgtcgg gcgcgttggc
    18661 gatgtcgtcc acggcgattg taagccggat tttgtcggaa aagacggaat tggggcagcc
    18721 gcacggtcag atggcgatgg gcgtgctgct gatgcaggac atcgccgtcg tgccgctgat
    18781 gattctgatt cccgcgctgg cgggcggagg ggacggaaat atttgggcgg ccttgggttt
    18841 ggcgtttgca aaaatgctgc tgacgctggg gctgctgttt ttcgtcggca gcaaaattat
    18901 gtcgcgatgg ttcaggatgg tggcaaaacg caaatcgtcc gaactcttta tgatcaatgt
    18961 gctgctggta accttgggtg tggcttatct gactgagctg gaaggtttgt ctatggcgtt
    19021 gggcgcattc gttgccggca tgctgctttc ggaaacggaa taccgtttcc aagtcgaaga
    19081 cgacatccgc ccgttccgcg atattttgct cggctttttc tttatcacgg tcggcatgaa
    19141 gctggacatt caggcattga tcggcggctg gcggcaggta ttgatgctgt tggcaatgct
    19201 gctggtgttg aaggcactgg ttgtgtttgc cattgccttc aaaatgaaac attcggtcgg
    19261 cgacagcctc aaaacggctt tgtatctcgc gcagggcggc gagttcggct tcgtgatgct
    19321 ggccattgct gggcagcttg atatggtttc gccagaatgg gaacaggcgg cgacggcggc
    19381 ggttctgctg tcgatgatta tcgcgccctt cctcttgggc ggcagcgatg cactggtcgg
    19441 gcgtttggtc aagtcaagct gggacatgaa gtcgctcgat ctgcacagta tgctggtaga
    19501 aaccatgagc aagtccgacc atgtgctgat tgtcggcttc ggcaggggcg ggcagacggt
    19561 cggacgcgtc cttgcccaag aggatattcc gtatttcgcg ctcgacttgg acatcgcgcg
    19621 cgtgcaggtt gccaaaaatg ccggcgagcc ggtgtcgttc ggcgatgcga aaaggcgtga
    19681 agtgttggag gcggccggtt tgggacgggc gaaaatggtg gtggttacgc tcaacaatat
    19741 gcacgaaacg caacacgttt tagacaatgt gctgtccatg tatcccaata tgcccgtata
    19801 tgtgcgcgcc accaacgacg attatgtgaa aacgtttacc gatataggtg cggaagaagc
    19861 cgtgtcggac accaaagaaa ccggactcgt gctggcaggc tatgcgatgt tgggcaacgg
    19921 cgcgtcgtat cggcacgtct atcagacgat ggcaaatatc cgccacagcc gttatgccgc
    19981 gttggaggga ctgtttgtcg gtagtgatga tgaggcagga ttcggcgaaa atggcgaaac
    20041 cgtccgccac gcctttcctt tggctgcaga agcatatgcc gtcggcaaaa cagtcggcac
    20101 gcttccgatg gcggcttacg gcatcaaact cttgttcgtc cgccgccgca ccggtcggat
    20161 tgaaaacccg gatgcctcgt ttacattgga aggcggtgac gtgttggtgg tcgcaggcaa
    20221 aaaagaagaa attatctctt ttgaaaactg gagtttgcag ggaatataaa tgaaatgccg
    20281 aaataaggct tgcgccattt ccggttattt ggtttaataa cgctttcgca aatcgcaagg
    20341 gtgattagct cagttggtag agcgtctgcc ttacaagcag aatgtcggcg gttcgactcc
    20401 gtcatcaccc accaagtttt ctttcattgt tgcaaacaat ggatgcgcgg tggtagctca
    20461 gttggttaga gtaccggcct gtcacgccgg gggtcgcggg ttcgagcccc gtccgccgcg
    20521 ccaaaagtta aggaatacca accggaggtt ggtatttttt tgtttgtatg ctttgaaaaa
    20581 tgtttgtttc cggattttgc cattcccatc cggttttgcg ctgtacgatg tgttttagcg
    20641 cggacttgct caaaatcgga tgtggttccg gtatttgagg ctttgattag ggatgcggac
    20701 tttcaatata tttttctcag ctacaacaac gaaggcttga tgtctgtcgg gcaggtaagg
    20761 gagatttttg agcgtttcgg caaatatgat ttggttcaaa cggaataccg gcggtttaag
    20821 gcagataaaa cagaaaaccg taaccataag gcaaattcga cattcgaata tctgcatatt
    20881 ttagaaaaga ccttttaaaa attcttgccg gatgctgcaa acaacgccgg tttgcattcc
    20941 tgatggcggt ggttttctta gacgaacgcc cgaacacgca ggaatggata ggcttggggc
    21001 tggttacggc gggcgtgttg acgctggcac tgaaacggta aagccgcaag aaataaatga
    21061 aatgccgtct aaaaaactgt tttcagacgg cattttcgtt tctgtccatc ctcagcactc
    21121 gaccacgcgc acggatacgg ggacggcttt tttccggagc gtggcggcga gtccggcaag
    21181 cgaggtttct ttgtaggtcg ccttcatatc ccggccggtt tgcagcatgg ttcggatgac
    21241 ttcgtcgagc gagacttttt tgtccgtgcc gtcttccaaa agcgcgagcg tgccgagttt
    21301 gagggctttt tcggcggcga tgccgttgcg ctcgatgcag gggatttgca ccagtccgcc
    21361 gacggggtcg caagtcagcc ccaaatggtg ttccatcgcc atttcggcgg cgttttccac
    21421 ttgtttgggc gtgccgccga tgacttcggc gtatgcgccc gccgccatcg aacacgctac
    21481 gccgacttcg ccctgacagc cgacatccgc accggaaatg gaggcgttgg tcttgtagag
    21541 gatgccgatt gcgcctgcgg tgagcaggaa gttttcgacg cgttcctgtg tggcgtgggg
    21601 gttgaacttg cggaaatagt gcaatacggc gggaatgatg cctgccgccc cgttggtcgg
    21661 ggcggtaacg acgcgtccgc cggcggcgtt ttcttcgttg accgccatgg cgtacaccat
    21721 cggccagagc tgggtgttga cgatttcggt ttcgcgcagg actttgagct tggcggcaag
    21781 ctgcggggcg cggcggcgga cgttcaatcc gccgggcagt tcgccgtccg cacccaagcc
    21841 gcgtttgatg cagccttcca taacctcggc aacggcagcg gcgcggcggc ggatttcggc
    21901 ttcgccgcat ccggcaagcg cggcttcgtt tgccaacacg acttcggaga tgtcgagccg
    21961 gttcagacgg catcgggcaa gcagttcggc gcaactggta tagggatagg gaacggcttt
    22021 ttccgtttcc gcctgccggt caaaatcttc ttcggtaacg acaaagccgc cgccgaccga
    22081 ataataaacc tgttcattca ataccgtgcc gtctgaagca taggcggtaa aacgcaggcc
    22141 gttggggtgt ttgggcagca cttgattgcc gagtatgttc aggtcgcggt cggggatgaa
    22201 gcggatttct tgcccgttga gccggaggat gtgctgcgtg cggatgcgtt cgaggcgttc
    22261 gggaatgccg acaagcggga tgtcgtgcgg caggctgcct tccaaaccga gcatcagcgc
    22321 gtcaaatgta ccgtgtccgt atccggtcag ggcgagcgag ccgtaaatgt cgatgacgat
    22381 gcgaacagcc tgtgcatcca aacctgccgc aaaggcggcg gctgccttca tcgggccgac
    22441 cgtatgcgaa ctggaaggcc cgataccgat tttgaaaata tcaaaaatgc tgaccatggt
    22501 tttgctccga aaggcggttt cagacggcat ggcttccgtt tgacaaacca aaaggagacg
    22561 cggcacgatg cccgtctcct tttttaaaac ggcacttatg cgtcgatatt ttgggcaatc
    22621 agcgcgttgt tttcgataaa ggcgcggcgc ggttcgacct cgtcgcccat cagggtaacg
    22681 aatacttcgt cggcggcaat ggcatcttcg atgcgcactt tcaacaggcg gcgcacggcg
    22741 ggatccatcg tggtttccca cagctgttcg gggttcatct cgcccaagcc tttgtatcgt
    22801 tggatggaca tacctttttg ggcaacgctc atcaagatgt ccaaagcgcc ttcaaagctg
    22861 tcgatgtcgt attcgttgtc gcctttgtag agcttcgccc ctttgccgac catgccttta
    22921 agtgcggcgg cggtttgggt aagggtttga taggctttgc tgttgaggaa cttaggctcg
    22981 aggtagctca ccaccacatt gccgtgcagc ttgcgcgtga ttttgatgaa ccggtgtcct
    23041 tcatgacctt cgatgcgttc gagggcgact tctttttcgt caagcagacc ggaaagttcg
    23101 gcaacggctt tatcggcgtt ttcagacgac gtcaaatcaa tgggcgacgc gtgtagcatg
    23161 gcgcgcagga cgagttcgtc tacgaagcgg ctttcctgtt cgatgacggt tttggccaac
    23221 aggaattgtt tggcggtatt ggcaagctct tcgccttcga tggtgcggct gtctgaaacg
    23281 attttggctt tttccaaggc taaaccgagc agccattggt ctttttccaa ttcgtctttg
    23341 agatatcgtt cttgtttgcc gtatttggct ttgtataaag gcggctgggc aatgtagatg
    23401 tagccgcgtt cgaccagctc gggcatttgg cggtagaaga aggttaaaag cagggtgcgg
    23461 atgtgcgcgc cgtccacatc ggcatcggtc ataatgatga tgcggtggta acgcagcttc
    23521 tcggcattga attcttcctt gccgatacct gcacccaacg cagtaatcaa agcggcgact
    23581 tcctgactgg cgagcatttt ttcaaagcgc gccttttcca cgttcaaaat cttgccctta
    23641 agcggcaaaa tcgcttggaa tttgcggtcg cgcccctgca tggcggaacc gcctgcagaa
    23701 tcgccctcga ccaaataaag ttctgacaat gcggggtctt tttcttggca gtcggcgagt
    23761 ttgccgggta gccccaagcc gtccattacg cctttgcggc gggtgatttc gcgggctttg
    23821 cgggcggctt cgcgcgcgcg ggcggcatcg acgattttgc cggtgatgat tttggcttcg
    23881 gcagggtttt cttcgaggaa gtcggtcagg gcttggctga tgacttcgtt gacgacggga
    23941 ccgatttcgc cggaaaccag tttgtctttg gtttgggacg aaaatttggg gtcgggcagt
    24001 ttgacggaca acacgcaggt caaaccttcg cgcatatcgt cgccggcggt ttccactttg
    24061 gcttttttgg cgacttcgtt ggcttcgatg tagctgttga tggtgcgcgt catcacttgg
    24121 cgcaacgcgg tcaggtgcgt accgccgtcg cgctgaggga tgttgttggt gaagcactgc
    24181 acgctttcct gatagctgtc gttccattgc attgcgcatt cgacgctcat gccgtctttc
    24241 tcgccgaacg cgtagaagat tttttcgtgc aagggcgttt ttttgcggtt catgtattgc
    24301 acgaatccag ccacgccgcc ggaaagggcg aagctttcgt gtttgccgtc gcgctcgtcg
    24361 gtcaattcga tgtccacgcc gttgttcagg aacgaaagtt cgcggatgcg tttggcaagg
    24421 atgtcgaagc tgtattcgac gttgccgaag gtttccgtac tggcgaggaa gcgcacggtc
    24481 gtgccttttt tatcggaatc gccgacaacg ttcagcggct cttcggtttc gccgcgcacg
    24541 aagcggacga agtgttcttt gccgtcgcgg tagatggtca gcgttaccca gtcggacagc
    24601 gcgttgacga cggacacgcc cacgccgtgc aggccgccgg agattttgta gctgttgttg
    24661 tcgaatttac cgcccgcgtg caatacggtc atgatgactt cggcggcgga gcgtccttct
    24721 ttcgggtgga tgccggtggg cataccgcgc ccgttgtcgg cgacgctgac ggaatggtcg
    24781 gcgtgtatcg ttaccgtgat tttgtcgcaa tgtccggcga gtgcttcgtc aatggcgttg
    24841 tccaatactt cgaacaccat gtggtgcaga ccgctgccgt cctgcgtgtc gccgatgtac
    24901 atgccggggc gtttgcgtac cgcttccaag ccttcgagca cctgaatgct gtcggcgccg
    24961 tattcttcgt gtttttgttc agtcatattt tttgccggat tttgaaaaga ttttgcgatg
    25021 ccgccaaaac aagtccgcac cttgtagaaa aagcgggcgg gacgacatat ataattgtgt
    25081 attatagccg attttgccgc ctaattcagc gttatccgca tcagtgtgcc gccgggaaaa
    25141 gatgaaacgg tacgtttgcc tccggcatca ggtcggggat tgtcccgtaa agtggcaaaa
    25201 gcgttttttt gccactaaaa tctacaccct atacttttcg gacaggggcg cggaaatgga
    25261 aatatggaat atgttggaca cttggctcgg tgccgtcccg atacgtgcgg aggcggtcga
    25321 atccgtggcg gcggttgcgg ctttgctgct ggcgcgcgcc cttctgttga atatccactt
    25381 caaacggcat ccggatttcg gcatcgaaag caagcggcgg tttttggttg ccagccgcaa
    25441 tataacgctg cttttggtgc tgttttcgct ggcatttatc tggtcggcgc aaattcaaac
    25501 gctggctttg tcgatgtttg cggtggcggc ggcggtcgtc gtggcgacga aggaactgat
    25561 tatgtgtctg tcgggcagta ttttaaggtc tgccacccag caatactcgg tcggcgacta
    25621 tatcgaaatc aacggcctgc gcgggcgcgt ggtcgacatc aacctgttga acacgctgat
    25681 gatgcaggtc ggcccgaacc ccttggtcgg acagcttgcg ggaaccaccg tttctttccc
    25741 caacagcctg ttgttaagcc accccgtgcg ccgcgacaat attttgggcg actacgtcat
    25801 ccatacggtc gaaatccccg tccccatcca tttggattcg gatgaggccg tatgccgtct
    25861 gaaagccgta ctcgagccct tgtgcgcgcc ctacatcccc gccatccagc ggcatttgga
    25921 aaacgtgcag gcggaaaaac tgtttatcac gcccgccgcc agaccgcgcg ttacccgcgt
    25981 accgtatgac gacaaggcat accgcatcat cgtccgcttc gcctcccccg tttcaaagcg
    26041 gctggaaatc caacaggcgg ttatggacga atttttgcgc gtacaatacc gcctgttaaa
    26101 tcatcccgcc gactccgaaa cactttaact ttccccgacc gaccccattt ccggcttcag
    26161 acggcatatt gccgatatgc tgtctgaagc acaacacgca aaggaaaccc atcttatgac
    26221 tgacaacgca ctgctccatt tgggcgaaga accccgtttt gatcaaatca aaaccgaaga
    26281 catcaaaccc gccctgcaaa ccgccatcgc cgaagcgcgc gaacaaatcg ccgccatcaa
    26341 agcccaaacg cacaccggct gggcaaacac tgtcgaaccc ctgaccggca tcaccgaacg
    26401 cgtcggcagg atttggggcg tggtgtcgca cctcaactcc gtcgccgaca cgcccgaact
    26461 gcgcgccgtc tataacgaac tgatgcccga aatcaccgtc ttcttcaccg aaatcggaca
    26521 agacatcgag ctgtacaacc gcttcaaaac catcaaaaat tcccccgaat tcgacaccct
    26581 ctcccccgca caaaaaacca aactcaacca cgatctgcgc gatttcgtcc tcagcggcgc
    26641 ggaactgccg cccgaacagc aggcagaact ggcaaaactg caaaccgaag gcgcgcaact
    26701 ttccgccaaa ttctcccaaa acgtcctaga cgcgaccgac gcgttcggca tttactttga
    26761 cgatgccgca ccgcttgccg gcattcccga agacgcgctc gccatgtttg ccgccgccgc
    26821 gcaaagcgaa agcaaaacag gctacaaaat cggcttgcag attccacact acctcgccgt
    26881 catccaatac gccgacaacc gcgaactgcg cgaacaaatc taccgcgcct acgttacccg
    26941 cgccagcgaa ctgtcagacg acggcaaatt cgacaacacc gccaacatcg accgcacgct
    27001 cgcaaacgcc ctgcaaaccg ccaaactgct cggcttcaaa aactacgccg aattgtcgct
    27061 ggcaaccaaa atggcggaca cccccgaaca agttttaaac ttcctgcacg acctcgcccg
    27121 ccgcgccaaa ccctacgccg aaaaagacct cgccgaagtc aaagccttcg cccgcgaaag
    27181 cctcggcctc gccgatttgc aaccgtggga cttgggctac gccagcgaaa aactgcgcga
    27241 agccaaatac gcgttcagcg aaaccgaagt caaaaaatac ttccccgtcg gcaaagtatt
    27301 aaacggactg ttcgcccaaa tcaaaaaact ctacggcatc ggatttaccg aaaaaaccgt
    27361 ccccgtctgg cacaaagacg tgcgctattt tgaattgcaa caaaacggca aaaccatagg
    27421 cggcgtctat atggacctct acgcccgcga aggcaagcgc ggcggcgcgt ggatgaacga
    27481 ctacaaaggc cgccgccgtt tttcagacgg cacgctgcaa ctgcccaccg cctacctcgt
    27541 ctgcaacttc gccccacccg tcggcggcag ggaagcccgc ctgagccacg acgaaatcct
    27601 catcctcttc cacgaaaccg gacacgggct gcaccacctg cttacccaag tggacgaact
    27661 gggcgtatcc ggcatcaacg gcgtagaatg ggacgcggtc gaactgccca gccagtttat
    27721 ggaaaatttc gtttgggaat acaatgtctt ggcacaaatg tcagcccacg aagaaaccgg
    27781 cgttcccctg ccgaaagaac tcttcgacaa aatgctcgcc gccaaaaact tccaacgcgg
    27841 catgttcctc gtccggcaaa tggagttcgc cctctttgat atgatgattt acagcgaaga
    27901 cgacgaaggc cgtctgaaaa actggcaaca ggttttagac agcgtgcgca aaaaagtcgc
    27961 cgtcatccag ccgcccgaat acaaccgctt cgccttgagc ttcggccaca tcttcgcagg
    28021 cggctattcc gcaggctatt acagctacgc gtgggcggaa gtattgagcg cggacgcata
    28081 cgccgccttt gaagaaagcg acgatgtcgc cgccacaggc aaacgctttt ggcaggaaat
    28141 cctcgccgtc ggcggatcgc gcagcgcggc agaatccttc aaagccttcc gcggccgcga
    28201 accgagcata gacgcactct tgcgccacag cggtttcgac aacgcggctt gatcgatgtg
    28261 aacaaaaaat gccgtctgaa acaatcaaac agtgtttcag acggcatcag catttgcgcg
    28321 gtttcgccat tttcacaaaa acagtaagcc aaaatcccgt cattcccgca aaaacaaaaa
    28381 atcaaaaaca gtgggcgtag ggtgggctga agcccaccct acatcccacc ttacatccca
    28441 ccttacggat tcgccctacg cccaaaaaat aaaaaatgcc gtctgaaacg gtcaaacaac
    28501 gcttcagaca gcattttttg gcagtcctta cccgatcagc cccaagtgcg acaagagcgt
    28561 ccatgtaccg atggcaatca acaccaaacc tccggcaaat tccgcacgcc tgccgaacaa
    28621 tacgcccaaa gcccttccca ccgtcagccc gaccgccacc agcaccgtcg tcgccatacc
    28681 gatgattgcg gcggcaaagg cgatgtttac ctccataaac gccaagccca ccccgactat
    28741 catggaatca atactggttc caaaagcagt cagaaccgtc aaccaccagc tttcccgttt
    28801 gttttcccgc gcgtcctccg cctcgccgga caacccttcg tgcatcattt tcagacccag
    28861 cccgcccagc aggacgaaag ccgcccaatg gtcccattcg ctgataaacg gcttggcata
    28921 aaaaccgcct acccagcctg ccagcggcgt gagcgcttca accgtgccga acaccaaggc
    28981 tgttgctgca attttgcgcg gaggcatcct gaccgccgca ccctttgcca gcgcgacggc
    29041 aaacgcatcc atcgacatcc ccagcgcaat caagagcaaa gcgtaaagac tcataccgca
    29101 cccgtcctta aaaagggcgg attatagcaa aacagcaaaa cgcgaaaaac gccgcgtaaa
    29161 gcagttttcg tgcgcggggc ggttcgcagg ggaatccgga accgtagggc ggatgcgtcg
    29221 gatggaacgt aggtgggact tagggcgggt tgtagggtgg gctgtagggt gggcttcagc
    29281 ccaccaattc tgccaaacca actcatccaa tcccgccaat tccaccgcat caagggtttt
    29341 tggcaaaccg tcgggattcg gcggtttcgg tttttggcgg ggattggcgg aatggttggt
    29401 ttgggttggt gggaatggtt ggattggcgg aggtggttgg attggtgggc tgaagcccac
    29461 cctacacatc caccctacag cccaccctac gcacctacgc ctcaccctac gtcccgcccg
    29521 actcatctgc cccgcgtccg tcctgcggat tttggcggcg gcgggagctt taccctcccc
    29581 aatccaaagg gtaaatgcct ttttcaacgt ctttgaagaa ggaagaataa cgccattcct
    29641 tcacactcgc cgcatacccg tgtttcacgg ggttgaaata aatataatcc cggtggttgt
    29701 ttaaatcttg ttcgtcccgg atcaggtgtt cccaaaacct gcgctgccaa atgccggctt
    29761 cgcgtttttt tgttttactc ccgttggggg cgcggcactc tttggggatg agttgggaaa
    29821 accgggtttt caaatatgca atccgtatcg ggtaatcgtg gtcgccttcc ggcagccgca
    29881 taatgaaatg caggtgatcc ggcaaaatac aaattgccgc cgtttcaaac ggataacgtg
    29941 ttttcgtttc acgataggcg aggcgcagcc tgtcgatatg ccgtatcagg taatccttcg
    30001 tcctgtcttg caaagccaat gtgaaaaaat agtgcgcccc gcgcgtgaag tccctccggt
    30061 aatttgacat atttatcccc tttttagccc gatgggcgga aacctgtatt attccttgtt
    30121 ttcagggcgt gcgggaggat ttttagggga tttgcaggga aatgggtagg gagggcgtag
    30181 ggctgatgtg taggctgtag ggtgtttcgt agggtgtttc gtagggtggg cttcagccca
    30241 ccattccgcc aatccaacca ttcccaccca acccaaacca accattccgc caatccccgc
    30301 cgaaactcga aacccgccga atcccaacgg ttcgccaaaa atccttgatg tggtggagtt
    30361 ggttgggttg gtggaatggt gggctgaagc ccaccctaca gcccacccta cgcacctacg
    30421 cctcacccta cagccctaca gcggcaaagg ggaatagaaa atgccgcccg agcggttcgg
    30481 gcggcatttg ttttcacggc ggggtcaaac ccgaccattg tccttattag cggcagatag
    30541 atggcaagaa ttttgctttt acagaacctg ctttacaagt ccactcgaac gagcctgagt
    30601 tttgtttgcc ttccagtacc aaggttgcgc cgctgatgtc tttgtttaca ccctgacttt
    30661 tcattgtaga agtaatggta gcacccgaaa cttctacctt agccacatac ttacctgtaa
    30721 tctcagaagc actggcaata cctgcggaag cattgttttt cgggaatgtg cctttgtcgg
    30781 aataatactc gaccactgcg gttttttgac cttcggccaa agtcagggct tcggacattt
    30841 gggcgcgcgc ggtgtagtct tggtatgcgg gcagggcgac ggctgccaag ataccgacga
    30901 tggcgatgac gatcatcagc tcgatcaggg tgaaaccttt ttggattgct ttcatttgga
    30961 tgactcctgt ttgaaaatgt ttaagtttga ttggaaattt gcggcatctg ccgcaaacgt
    31021 aaacccgtaa tgtatttgac cgtggtcttt tttgtttcgg ttcgccttct ttccagcaag
    31081 cggcgtgcca aaacggggtt cagctcggca ttttacagaa tattttttta tttgtctgtt
    31141 tggtattggt atttttttat atcaacgcgg cgcggcctgc ggcgggctga cgtttttttg
    31201 tcaattgtga caatttttgt cagtttcggg ctgacgtttt ttgtcagatt ttgtcagatt
    31261 gggttttttt tggttttttt gggggtgggg tgggattggc ggaatgacga aatataagtt
    31321 tccgtgcgga cgggtccgga ttcccgcctg cgcgggaatg acggatctgt ccgcacgaaa
    31381 acccgtatcc cgtcattccc acgaaagtgg gaatctagaa actcaaagct gcaagaattt
    31441 atcagaaatg actgaaactc aacgaacctg gattcccact ttcgtgggaa tgacggagga
    31501 gaggttgctg ttttcccgat aaatgcccgc aatctcaaat cccgtcattc ccgcaaaaac
    31561 aaaaaatcaa aaactgaaat ttcgtcattc ccgcgcaggc gggaatccag aaactcaaag
    31621 ttgcaagaat ttattagaaa tggctgaaac tcaacgaacc tggattcccg ctttcgcggg
    31681 aatgacgcgg aaaagttact gtgatttcgg ataaattttc gcaacgctta atttccgttt
    31741 tacccgataa atgcccgcaa tctcaaatcc cgtcattccc caaaaacaaa aaatcaaaaa
    31801 caaaaatttc gtcattcccg cgcaggcggg aatctagagt tgtcggtacg gaaacttatc
    31861 ggaaaaaacg gtttctttag attttacgtt ctagattccc gctttcgcgg gaatgacgcg
    31921 gaaaagttgg ctgtggggcg ggaatggcgg gctgtagggt gggcttcagc ccaccgcctc
    31981 cgccaacttc agacggcata ggtttgccgg caccggtaag attgtcggat ttgtcggatt
    32041 tgtcgaggtg gcggatttgt cgaggtggcg gattggtgtg attggtgtga ttggtgtgat
    32101 tggtgggctg aagcccaccc tacgaacacc ctacgtccca ctgcgatgcg cgaccatata
    32161 aaaatgcggg gtggggtgtg gaaatgccgt ctgaagcggt caaacgacgt ttcagacggc
    32221 atttttatgc ccggttattt ccggtagcgg acggcgcggg acaggatttc ttcaatttcc
    32281 atccacataa tgccccctta cagcaaaccg ggctggccga gtgcggggtc ggtcgcgcgg
    32341 gcggcttgtg cgtcttcgac agtcagtccg agggcttttg ccacgccttc gccgtatgcc
    32401 ggatcgcaac ggtagcagtt gcggatatgg cggtatttga tgaagtcggg cgcgtcgccc
    32461 attgcggcgg cggtgttgcc gaacaatgcc tgtttctgcg cgtcgttcat caggttgaac
    32521 agggcgcgcg gttggctgaa atagtcgtca tcgtcttggc ggtagtccca gtgcgccgcg
    32581 tcgccgttga ttttcaaagg cggttcggcg aagtcgggct gctgctgcca ttggccgaag
    32641 ctgttgggtt cgtagtgcgg caggctgccg tagttgccgt cggcgcgtcc ttgtccgtcg
    32701 cgctggttgc tgtgaacagg gcaacgcgga cggttgacag ggatttggcg gaagttcacg
    32761 cccaagcggt agcgttgcgc gtcggcgtaa ttgaacaaac gcgcctgcaa cattttatct
    32821 gggctggcgc cgacaccggg aacgaggttg ctcggtgcga aggcggattg ttccacatcg
    32881 gcgaagaagt tttcgggatt gcggttcaac tcgaattcgc ccacttcaat cagcggatag
    32941 tcttttttcg gccaaacttt ggtcaagtca aacggatgat aaggtacttt ttccgcgtct
    33001 gcttcaggca tgacttggat gtacatcgtc catttcggaa actcgccgcg ctcgatggct
    33061 tcgtacaggt cgcgctgatg gctttcgcgg tcgtcggcga tgattttggc ggcttcttcg
    33121 ttggtcaggt ttttaatgcc ttgttgggtg cggaaatgga attttaccca aaaacgctcg
    33181 cctgcttcgt tccagaagct gtaggtatgc gaaccgaagc cgtgcatatg gcggtagccg
    33241 gcggggatgc cgcggtcgct catcacgatg gtaacttggt gcagtgcttc gggcagcagc
    33301 gtccagaagt cccagttgtt tgtggcagag cgcatattgg tgcgcggatc gcgtttgacg
    33361 gctttgttca ggtcagggaa tttacgcggg tcgcgcagga agaacacggg cgtgttgttg
    33421 ccgaccacat cccagttgcc ttcttcggta taaaatttca aggcaaaacc gcggatgtcg
    33481 cgttctgcat cggctgcgcc gcgttcgcct gccacggtgg tgaaacgggc gaacatctcg
    33541 gtttttttgc cgacttcgct gaagattttg gcgcgggtgt atttggtgat gtcgtgcgtt
    33601 acggtaaacg taccgaacgc gcccgaacct ttggcgtgca tacggcgttc ggggatgact
    33661 tcgcgcacga agtcggcgag tttttcattc agccacaaat cctgcgccag cagagggccg
    33721 cgaggaccgg cggtcaggct gttttgattg tcggcaacag gcgcgccgtt gttcatggtc
    33781 agatgggtta cagggcattt ggaggtagtc atcgctcttg ttccttttct caggttggtc
    33841 aaatgggggt aaacggctta cagtacgatt tggcggaaag cgtattcgta accggtttct
    33901 tgattgcaat aaatttcttg aatcgacatt ttatttccct tttgtaaaaa ctatggatgc
    33961 gactatacgc caagattttc gctattaaaa ctatgaaatc gatttaatat tgttataagc
    34021 aatcggtttt tgattttcgt ttgttttttg ttatcggcac gaatcaaaat ccctacataa
    34081 aaccatttat aataatgttt cccggcatca tctgccaccg acaggccaaa ataataattc
    34141 tctcgcatta tcatgacgtt actcggaata aagctcaaac agacccagca gctcaaccag
    34201 cggctgcaac aatctttgcg cgtattgcag atgtcgggta tcgaacttga acgcgaggtc
    34261 gaaaactggc tgtcggacaa ccccctgctc gaacgcaaag atacggatga attttccgat
    34321 gccgagttca gccattacac tgcgcctgcc cgtcaaatcg gcggagacga aggcgaagat
    34381 atgctgtcca acatcgccgg cgagcaggat ttcaagcaat acctgcacgc gcaagtatgc
    34441 gaacacccgc tttccgatca agaatccgcc tgtgtccaca tcctcatcga tttccttgac
    34501 gagcaaggtt atctgaccga cagcatcgaa gacatcctcg accatacgcc cttggagtgg
    34561 atgttggatg aagcaatgct gaaacaagcc ctgaccgcat tgaaaaaatt cgacccggca
    34621 ggcgtggccg ccgccgattt gaacgaatcg ctgatactgc agatagaaag atcgggcgaa
    34681 tgtgctgcca aaccctccgc cctgcatatc gtccgaaacg ccctcgacag cattgacggc
    34741 aaccgcagcc aaaccctcgc gcgaataaaa aaacgcctgc cccaaaccga cagcggcaca
    34801 ctcgaagccg cactcgacct cattgcttcg ctcaatccct ttcccgccgc tggttttgcc
    34861 tcgtccacac ccacgccgta ttctgacgag gcgctcgcca acctgctggc tttccgcggc
    34921 atggaggttt ctcgccgcac cattgccaaa tacagagaat cctttgagat tccggcagca
    34981 cacaaacgca aaaccgcaga ataattgctg aataatctta taaagacaac aaaccaaaag
    35041 ccggcatttc tgcgaaagcg ggaatgccga atccgtccgc gcggaaacct gcatcccgtc
    35101 attcccgcga aagagggaat ctagaaacgc aaag
//
LOCUS       DAWFLT010000013        33302 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWFLT010000013 DAWFLT010000000
VERSION     DAWFLT010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.013"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(107..1030)
                     /gene="era"
                     /locus_tag="WGR74_000612"
     CDS             complement(107..1030)
                     /gene="era"
                     /locus_tag="WGR74_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ0905089.1"
                     /translation="MDIETFLAGERAADGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELIKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="WGR74_000613"
     CDS             complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="WGR74_000613"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ0905090.1"
                     /translation="MKDDVLKQQAHAAIQKKLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFAERVRRVDFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            1963..2286
                     /locus_tag="WGR74_000614"
     CDS             1963..2286
                     /locus_tag="WGR74_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ0905091.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNQPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADITKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPKD"
     gene            2333..2809
                     /locus_tag="WGR74_000615"
     CDS             2333..2809
                     /locus_tag="WGR74_000615"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ0905092.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            2887..3312
                     /gene="nusB"
                     /locus_tag="WGR74_000616"
     CDS             2887..3312
                     /gene="nusB"
                     /locus_tag="WGR74_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ0905093.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(3678..4712)
                     /gene="pyrC"
                     /locus_tag="WGR74_000617"
     CDS             complement(3678..4712)
                     /gene="pyrC"
                     /locus_tag="WGR74_000617"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ0905094.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPALVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHISTAEAARLVLEAGDNVAASVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAVELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENTDTITLVKQSQTVPASIPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(4772..5377)
                     /locus_tag="WGR74_000618"
     CDS             complement(4772..5377)
                     /locus_tag="WGR74_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905095.1"
                     /translation="MPSEMRMSRLMLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLF
                     RSACVLTQGSFESVSKFAAVGHFVPVGKEDLSHLPPTVAEPDVQALWTLERDGGIYYL
                     SLTRDSCSVKAERADSAALLEHFDGLVRQPPKNANIELRAEQSVQASFEIRQVSYAWR
                     EAGSSEETVLTAQTADSPDLPVQAVLNLTHRSHNGKPLILP"
     gene            complement(5378..5602)
                     /locus_tag="WGR74_000619"
     CDS             complement(5378..5602)
                     /locus_tag="WGR74_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ0905096.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVQHK"
     gene            5711..6322
                     /locus_tag="WGR74_000620"
     CDS             5711..6322
                     /locus_tag="WGR74_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ0905097.1"
                     /translation="MRRAILLILALTAGTSLATSFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNDKLSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSIKPHDK"
     gene            complement(6382..7254)
                     /locus_tag="WGR74_000621"
     CDS             complement(6382..7254)
                     /locus_tag="WGR74_000621"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ0905098.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPTDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(7291..8076)
                     /locus_tag="WGR74_000622"
     CDS             complement(7291..8076)
                     /locus_tag="WGR74_000622"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ0905099.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAKAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESV
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAVVGALVKELKDAVR"
     gene            complement(8503..8595)
                     /locus_tag="WGR74_000623"
     tRNA            complement(8503..8595)
                     /locus_tag="WGR74_000623"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8559..8561),aa:Ser,seq:gct)
     gene            complement(8752..8949)
                     /locus_tag="WGR74_000624"
     CDS             complement(8752..8949)
                     /locus_tag="WGR74_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905100.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDVAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            complement(9054..9371)
                     /locus_tag="WGR74_000625"
                     /pseudo
     CDS             complement(9054..9371)
                     /locus_tag="WGR74_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            complement(9394..10155)
                     /gene="kdsB"
                     /locus_tag="WGR74_000626"
     CDS             complement(9394..10155)
                     /gene="kdsB"
                     /locus_tag="WGR74_000626"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ0905101.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAV
                     RVVVATDHPDIQTACQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPSETAVLRHIGIYAYRAGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            complement(10152..10334)
                     /locus_tag="WGR74_000627"
     CDS             complement(10152..10334)
                     /locus_tag="WGR74_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ0905102.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALSEEELKA"
     gene            complement(10403..10984)
                     /locus_tag="WGR74_000628"
     CDS             complement(10403..10984)
                     /locus_tag="WGR74_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ0905103.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAKAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            complement(11190..12224)
                     /locus_tag="WGR74_000629"
     CDS             complement(11190..12224)
                     /locus_tag="WGR74_000629"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ0905104.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNEKSRAEDAGDEPLLLFRKTVAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            complement(12264..12535)
                     /locus_tag="WGR74_000630"
                     /pseudo
     CDS             complement(12264..12535)
                     /locus_tag="WGR74_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12771..14051)
                     /locus_tag="WGR74_000631"
     CDS             complement(12771..14051)
                     /locus_tag="WGR74_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ0905105.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGQRVLADAVKGKDIFLGLSGANLLTPEIL
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            complement(14273..14893)
                     /locus_tag="WGR74_000632"
     CDS             complement(14273..14893)
                     /locus_tag="WGR74_000632"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ0905106.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAVIDSNLGLDEVRNSIEKVLDRHFGC"
     gene            complement(14953..15948)
                     /gene="mltG"
                     /locus_tag="WGR74_000633"
     CDS             complement(14953..15948)
                     /gene="mltG"
                     /locus_tag="WGR74_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ0905107.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            16032..16604
                     /gene="ampD"
                     /locus_tag="WGR74_000634"
     CDS             16032..16604
                     /gene="ampD"
                     /locus_tag="WGR74_000634"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ0905108.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPNGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDDMAYH
                     AGVSSFGGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            16798..18084
                     /locus_tag="WGR74_000635"
     CDS             16798..18084
                     /locus_tag="WGR74_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ0905109.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            18232..20757
                     /gene="ligA"
                     /locus_tag="WGR74_000636"
     CDS             18232..20757
                     /gene="ligA"
                     /locus_tag="WGR74_000636"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ0905110.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGKPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVANIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEQMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMRETAVAVSDGIGHRQDDLFAET
                     PSANQTQSVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKEAADKTVAESDQMPSEG
                     SSVGASGKHKKQPTKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPA
                     AQYAEPQRWIARLPGFKISENKAQALWELAGKSIEGLQTDKSLPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            20814..21989
                     /locus_tag="WGR74_000637"
     CDS             20814..21989
                     /locus_tag="WGR74_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ0905111.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     ATALKLFDKINIDLMYALPNQTVRTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDTVHGALENAGFIHYETSAFAKPAMRCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPTAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLNDLLQCFL"
     gene            22209..22469
                     /locus_tag="WGR74_000638"
     CDS             22209..22469
                     /locus_tag="WGR74_000638"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905112.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKNRMPAPATIRQIP
                     LFFPNRLQVSPIRRVMPPPPFKGNARVNGFAVGKAAGYRYVS"
     gene            22576..23100
                     /gene="nspA"
                     /locus_tag="WGR74_000639"
     CDS             22576..23100
                     /gene="nspA"
                     /locus_tag="WGR74_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ0905113.1"
                     /translation="MKKALATLIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSIGLGVLTGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSVGVRVKF"
     gene            23236..23625
                     /locus_tag="WGR74_000640"
     CDS             23236..23625
                     /locus_tag="WGR74_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0905114.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            23703..24533
                     /locus_tag="WGR74_000641"
     CDS             23703..24533
                     /locus_tag="WGR74_000641"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ0905115.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRITKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLKGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            24548..26005
                     /locus_tag="WGR74_000642"
     CDS             24548..26005
                     /locus_tag="WGR74_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ0905116.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(26046..26435)
                     /locus_tag="WGR74_000643"
     CDS             complement(26046..26435)
                     /locus_tag="WGR74_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905117.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLL"
     gene            complement(26583..26972)
                     /locus_tag="WGR74_000644"
     CDS             complement(26583..26972)
                     /locus_tag="WGR74_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905118.1"
                     /translation="MWKIIKEDSDDLEFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDGGIGDIDNIVDFVSSYSLPKSKKNALTGIAFLRGIDVYDPPISKEK
                     ALKALKKHPEIYQKFQHFFPFVELPPL"
     gene            complement(27094..27483)
                     /locus_tag="WGR74_000645"
     CDS             complement(27094..27483)
                     /locus_tag="WGR74_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905119.1"
                     /translation="MWKIIKEDSDDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDVEIGDIGNIVGFAPSSSLPKSKKNALTGIAFLRGIDVYDPPVSKEK
                     ALKALEKYPEIYQKFQHFFPFVELPPL"
     gene            complement(27584..27982)
                     /locus_tag="WGR74_000646"
     CDS             complement(27584..27982)
                     /locus_tag="WGR74_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905120.1"
                     /translation="MLGLTRDNCYDLGFAILCYERNAADKKEFLDWLYGIVRTVPAES
                     FPRYLIDLIDCLESDDCSINIYNIIGFVPTTGMTKKEKDAVYGITYIRNQGFYDIPIT
                     REKALKALKKHPEIYQKFRHFFPFVELPPL"
     gene            complement(27985..28176)
                     /locus_tag="WGR74_000647"
     CDS             complement(27985..28176)
                     /locus_tag="WGR74_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0905121.1"
                     /translation="MRLSDGIFDTKQVTKGVFDGKGEAKIPSYTWHHHQDTGRMQLIS
                     ERKHTQTGHIGGEAMDKGK"
     gene            complement(28237..28638)
                     /locus_tag="WGR74_000648"
     CDS             complement(28237..28638)
                     /locus_tag="WGR74_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905122.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEENNSDGNPTANYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(29055..29384)
                     /locus_tag="WGR74_000649"
     CDS             complement(29055..29384)
                     /locus_tag="WGR74_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905123.1"
                     /translation="MTDKSKTEKLISSDDKQSVIDGILDMVFNSKAYEVPWISEKLME
                     LSKNKDLDIAGLSLTCFGHLARLHSNIGDYDKVISLLHSKQDDPELQGRAEDALEDIS
                     LFLSENH"
     gene            complement(29410..30117)
                     /locus_tag="WGR74_000650"
                     /pseudo
     CDS             complement(29410..30117)
                     /locus_tag="WGR74_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253165.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
     gene            30073..30216
                     /locus_tag="WGR74_000651"
     CDS             30073..30216
                     /locus_tag="WGR74_000651"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905124.1"
                     /translation="MNMGNGFSAVCRFDQGAAVEGAGVGFLKNSVLNILLQMKERPSE
                     KQA"
     gene            complement(30252..30794)
                     /locus_tag="WGR74_000652"
     CDS             complement(30252..30794)
                     /locus_tag="WGR74_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ0905125.1"
                     /translation="MINHIEKYIGTISHGSKSDSGSQYKLNIAAIPSSPNRDLKTYIT
                     LGLSKHDLNYKSRFEILFVCSLKYDENQIFPFLRWLAETIIENKKILLRGQVVYLPRS
                     IVDSTKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFEQ
                     FLLNNEVDNLSDMKRKPFAW"
     gene            complement(30921..31148)
                     /locus_tag="WGR74_000653"
     CDS             complement(30921..31148)
                     /locus_tag="WGR74_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905126.1"
                     /translation="MDIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            complement(31207..31530)
                     /locus_tag="WGR74_000654"
     CDS             complement(31207..31530)
                     /locus_tag="WGR74_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEZ0905127.1"
                     /translation="MIVDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     QLISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            complement(31533..32240)
                     /locus_tag="WGR74_000655"
     CDS             complement(31533..32240)
                     /locus_tag="WGR74_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ0905128.1"
                     /translation="MVKTADGYKAIARIRTGDRVFAKDEASGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNNQTLISNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGNQAETEGVWVHNDCPYGEKYRTEVGSYTNTHESGRT
                     YSGKGTRQRSQISGAREARVNNDPHIATDFTPAKNNREAFKDESRRIDAHGGSKSTNN
                     YNRIESPGKKYRKQDGD"
     gene            complement(32374..32691)
                     /locus_tag="WGR74_000656"
     CDS             complement(32374..32691)
                     /locus_tag="WGR74_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905129.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            complement(32694..>33302)
                     /locus_tag="WGR74_000657"
     CDS             complement(32694..>33302)
                     /locus_tag="WGR74_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233664.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HEZ0905130.1"
                     /translation="PAEGKFAVIGGLGSVAGFEKNTREAVDRWIQENPNAAETVEAVF
                     NVAAAAKVKNLTKAAKPGKAAVSGDFSDSYKHNTASRLSQSVDGEMFQTRNVDFKAKS
                     IGTKIHDGAQGKHISGHGNYIEGKSTLNQNINPQELLNGIHSGAYPVISKGARGNPVV
                     DFGYPIGSDGKSGLSTNFGTIHSGKNGVHIVPANPKTIKKVQ"
BASE COUNT         8195 a         9378 c         7590 g         8139 t
ORIGIN      
        1 tgcacagata atatcaaccc gtttttaaca aagatattcc cgattgaaac aaaatgccgt
       61 ctgaaacctg ttcggacggc atttgcgtaa agttcagcaa gaaaaactac aaacccagct
      121 cgcgcaggaa gcggatgtcg tccgcccaac cggatttgac tttgacccag accttcaaaa
      181 atactttggt atcaaacagt ttttccatat ccaaccgcgc ttcggtggaa attttcttca
      241 aacgctcccc gcctttgccg attaaaatcg ccttttggct ttccttatcg accaaaacgg
      301 cgatatagat gcggttcaaa ccgtcttcct cttcaaactg ctccacttcg acgttcatcg
      361 cataaggcaa ttcctcgccc aaatagcgga acaatttttc acgcacgatt tccatcgcta
      421 aaaaacgcgc cgatttgtcc gtaaccatat cttcgggata catcggcacg ctttcgggca
      481 gatacggctt aatcagctcc aacaggttgg caatccgcaa tccgtgtttc gcgctgaccg
      541 cctccgccgc cgcaaattca aattcggcgc gcacctgggc aacaaacgcc tccagcgcgt
      601 aacggtcttt cgccttgtcc ttatcgattt tgttgaccac taaaatgacc ggcgtgtgct
      661 tgggcagttg tttcaacacg acgcggtcgg catcggtaaa acgcatcgct tccacgacga
      721 aaaccaccac atccacgccg ccgagtgcct cggtaacgtt ttgattcaaa cggtcgttga
      781 gcgcgttgcg gtggtcggtt tgaaaaccgg gcgtatcgac aaacacaaac tgcgcggtat
      841 cgtcggtata aatccccgtt acgcggttgc gcgtcgtctg cgcctttttg ctggtaatac
      901 tgattttctg accgatgaga tgattcatca gcgttgattt gccgacattc ggacggccga
      961 caatcgctac gaagccgcaa cggtatccgt cggcggcgcg ttcccctgca aggaaggttt
     1021 caatatccat atttgctttc gtatccatat ttcagacggc atattcacgc gccgcctcat
     1081 ttctttttct tcttcagcgg cagcttctct tccagccatt tcaaagcctc tttcgccgct
     1141 tcctgctccg ccgccttgcg gctcgtccct ttggcacggc acacgaaacc cagttcgccc
     1201 aaatcgcagg aaatgacaaa catactgtcg ttggcatggc cgatttgctc ttcgatacgg
     1261 tatttcggca aggcgaaacg gcgcgcctgc aacgcctcct gcaaagcagt tttgccgtct
     1321 tttgcctgat tttggaaatc gactcgccgg acgcgttctg caaacaaatg gcgcaccacc
     1381 ttttccgccg tgttgaaatc cgcatcgaag ctgaccgccg caaacatcgc ctccatcgcg
     1441 tccgccagta tcgaaggccg tctgaagccg ccgctcttca actcgcccgc ccccaagtac
     1501 aggccgtcgc cgacattcat ttccgccgcc atttccgcca gcacgccctc attgaccaga
     1561 cttgcccgca accgcgacaa ctcgccctcg gtcaacttcg gaaacgcgtc aaacagcatc
     1621 cgcgccaccg tataattcaa aatcgaatcg ccgacaaact cgaaccgctc gttgtgcttc
     1681 gcatgatggc tcctgtgcgt caaagcctgc cgcaaaagcg aaatatcgcg gaacgcgtag
     1741 cccagtttct tctgtatcgc cgcgtgtgcc tgctgtttca aaacatcgtc tttcattacc
     1801 gtcttccgca aaaaacagcc cgaatgcgtt tccatactcc aaaaatacag aaacacctta
     1861 aggctgcaaa cggcgcacat tgtaccacca aaccgccgcc gtctgccgac aacgcaagaa
     1921 tcgtggtaaa atccgcaagt tttcccccaa cgtattcccg caatgaacgc atttgcacaa
     1981 gcactttcct cggcactcga ccgctgcatc gccaccaaca ccgtcgccaa acaaaaccag
     2041 cccgtcggct tcctctaccg cgaagccccc gtttttgaaa acgacagcgg ctggcgtttc
     2101 ttcagcggcg acgagaccga cgaatacacc gacgacccgg acaacttcag catcgtcagc
     2161 ctcgccgata tcaccaaaac caaccctgaa accgccgcac tgctctccca acctgaaggc
     2221 agcgcgtggg aattggcaga agacggcaca ttccaaaccg tcgccgactg gcaacctaag
     2281 gactgaccct tcagacggca ttccgaaacc tccgaatacc aaaggacact caatgaacac
     2341 catcgccccc aacctcgacg gcaaacacct ccgcatcggc atcgtacagg cacgcttcac
     2401 caacgaaatc ggcagcgaaa tgctcaaagt ctgctgccgc accctccaag aattgggcgt
     2461 ggcagacgaa aacattaccg tcgccaccgt acccggcgcg cttgaaatcc ccatcgcgct
     2521 gatgaacttt gcctcttctg aaaaatttga cgcactgatt gccatcggcg tcgttatccg
     2581 tggcgaaacc taccatttcg agctggtttc caacgaatcc ggagcagggg tcagccgcgt
     2641 cgcactcgac tacaacatcc cgattgccaa tgccgtcctg accacggaaa acgacgcaca
     2701 ggcaatcgaa cggattgaag aaaaagcctc ggatgccgcc aaagtcgccg tagaatgcgc
     2761 caacctcgtc aacctcctgc tcgaagaaca gtttgaagac gaagaataac aaacagggcg
     2821 tacaccgata cgccctttcc gtttcagacg gcataccgtg ccgcccaacc gtttcaagga
     2881 aaaatcatga aaacagcccg ccgccgttcc cgcgagcttg ccgtacaagc cgtttaccaa
     2941 tcccttatca accgcaccgc cgcgcccgag attgctaaaa acatccgcga aatgcccgac
     3001 tttgccaagg cagacgaaga attgttcaac aaacttttct tcggcacgca aaccaatgcg
     3061 gcagagtaca tccgacaaat ccgccccctg ctcgaccgcg acgaaaaaga cctcaacccc
     3121 atcgaacgcg ccgtcctgct gaccgcctgc cacgagctgt ccgccatgcc cgaaacgccc
     3181 taccccgtca tcatcaacga agccatcgaa gtaaccaaaa ccttcggcgg cacggacggg
     3241 cacaaattcg tcaacggcat cctcgacaaa ctcgccgccc aaatccgtcc cgacgagccc
     3301 aaacgccgtt gatgcatcac aaatttaaag aaagttctat gtccttattt ctgctttctt
     3361 tgtttcgtca ttcccacgca ggtgggaatt tggttttttg aattttgggt atttcaaata
     3421 aattctgaaa cttcaatctc gtcattcccg cgcaggcggg aatccggttt tttgagttgc
     3481 tgccgttttt gataaactct ggtcgcatcg aatttccgga ttcctgcctt cgtgggaatg
     3541 gcgaaatttg aaaattgtgg gaatttatcg gaaataaccg aaacgtaaca aactcaaacg
     3601 ccgttgacgt atcaatcagt caacaacaca aatgccgtct gaaaaacctt tcagacggca
     3661 ttttgtttcc agccccatca atactgtacc gtccagccga tttcgccgcc cgcgcgcatc
     3721 gggacaagtt cgccgtcgcc gtagggaata cttgcgggga cggtttggct ttgtttgacg
     3781 agggtgatcg tgtcggtatt ttcaggaatg ccgtaaaacc ttgcgccatt tttcgaggca
     3841 aaggcttcga gtttgtccaa cgcgcctgct ttttcaaaca cttcggcgta aagctcgacg
     3901 gcggtcatcg cgctgaacat accggcgcaa ccgcaggcgt tttctttggc ggatttggcg
     3961 tgcggcgcgg agtcggtgcc gaggaagaat ttatgcgcct tctcgccggt aacggcggca
     4021 accaatgcct gacggtgggt ttcgcgtttg agtacgggca ggcagaaatg atgggggcgc
     4081 acgccgccga ccaagaggtc gttgcggttg agcaggaggt gttgcggggt cacggaggcg
     4141 gcaacgttgt cgcccgcttc caaaaccagg cgggcggctt cggcggtgct gatgtgttcg
     4201 aacacgactt taagattcgg cacttgcgcc aaaacgggtt tcatcacgcg cccgataaag
     4261 gcggcttcgc ggtcgaagat gtcgatttcg gggtcggtta cttcgccgtg aacgaggaac
     4321 aaaatgccct gtttcgccat ttcttccaac acggggatga gcttgaacag gtcggttacg
     4381 ccggaatcgg aattggtggt cgcgccggca gggtagagtt tgaaggcgac gatgccggcg
     4441 gctttggctt cgcgcacaag tgcaggcgtg gcttggtcgg tcaggtaaag cgtcatcaac
     4501 ggctcaaacg cgctgccttc gggcaatgcc gccatgatgc gcgctttgta ggcaagcgcg
     4561 tcggcaacgc tgacgacagg cggtttgagg ttgggcataa tgacggcacg ccccatttgg
     4621 cgggcggtat aaggagcaac ggctttgagc gcgtcgccgt cgcgcaggtg caggtgcata
     4681 tcgtcggggc ggataatggt cagggtttgc atggttttcc tttgttttat ttgaatgttc
     4741 agacggcatg ccgactttat gccgtctgaa gttaggggag aattaggggt ttcccgttgt
     4801 gggaacggtg cgtcaggttc aaaactgcct gtacgggtaa atccggggaa tcggcggttt
     4861 gtgcggtcaa tacggtttct tcggagctgc ccgcttcccg ccaggcatag ctgacttggc
     4921 ggatttcaaa tgatgcctgc accgactgtt ccgccctcaa ttctatattg gcatttttcg
     4981 gcggctgtcg gacaagtccg tcaaaatgtt ccaacagcgc ggcactgtct gcccgctcgg
     5041 ctttcacact gcaactgtcg cgcgtcaggc tcaaataata aatgccgcca tcgcgttcaa
     5101 gcgtccacaa cgcctgtaca tcgggttctg caacagtggg cggcagatgg gacaggtctt
     5161 cctttccgac agggacaaaa tgtccgacag cggcaaactt ggaaaccgat tcaaaactgc
     5221 cttgagtcag gacgcaggca ctccgaaaca aatcgacggc tacacgcgcg aaatcctgat
     5281 gccgtacacc ggattgccgg ttttgggcat tacacgcagc aagcacaaat gccgacagca
     5341 ataacatcaa tcgggacatc cgcatttcag acggcattta tttgtgttgg acgaccagcg
     5401 tgaacacgcc gtcctgttcg gaagcatcca acagcacatg accggtttgg cggcaaaaag
     5461 cctcaaaatc ccccggtgcg ccgccgtcgg ttgccagaac ggtcaacacg tcgccctgct
     5521 gcatttgcgc caaagccttt ttcgcccgca aaatcggcag gggacatttc aatccggtta
     5581 cgtctaaagt ttcgctgttc atcgtttgta ccgtttaaaa aatgggaatc ggggggagat
     5641 tataccccag ttgcttgcag ccccctttac caacccataa aataacgccg ttttcaattt
     5701 ggagagatct atgcgccgcg ccatccttct gattctggcc ctgactgccg gcacgtccct
     5761 tgccaccagc ttcagccaaa aagacacgcc gatcaacacc cgctaccgcg aaacgcccga
     5821 agaagccgcc gcccgcgaat ttaaagaaca caccgccaaa ctgccgccgc tgcccgatac
     5881 gcactcggac ggctggtttg acatttatgt ggatgaaaat tacggcaaac agccgaaaat
     5941 cctgctcgac agcctgcaaa ttatgcccgc gcccgacggc agcatccgct acattctcaa
     6001 tatccgctcc gacaaaggct acgacaacct gactgccgaa ggcatattct gcgcccgctt
     6061 ctccatcagg ttcggcaacg acaaactttc ctcctacaaa gtgttcggct acggcgacac
     6121 ggtcaaccgc cgctggatac agccccgaaa cgccgaatgg aaaccgatag gcggtacatt
     6181 aagccgcaac gacgcactgc gcaccgtcct gtatcaggca ttctgcgagg gcggtgtacc
     6241 cgccgacacg aaaggtttgg tacaacgcct gaaagaacgt gccggacgct atgccccgtc
     6301 gataaagccg cacgacaaat aaccagacac aaaaataccg tctgaaacat cggtttcaga
     6361 cggtattcga ttcatcagcc atcaggcggc agaaactttg tcctgacggc acaacagcgt
     6421 aatcaaatca ctgatgcgcc gcttcatatc gcggcggtcg acaatctggt cgattgcgcc
     6481 tttttccagc aggaactcgg cgcgttggaa gccttccggc agcgtttcgc gcaccgtctg
     6541 ctcaatcacg cgcggaccgg caaaaccgat cagcgcgttc ggttcggcaa gcacaacatc
     6601 acccaaaaat gcgaagctgg cggatacgcc gcccatagtc ggatcggtca acaccgatat
     6661 aaacggcagg cgtttttccg tcagcaaatg cagcgcggca ctggttttcg tcatctgcat
     6721 caatgagttc acaccctcct gcatacgcgc accgccggaa gccgccacac agacgaacgg
     6781 acaattgtcg gcaaccgccc ggcggacgcc ctgtacgaat cgttcgccca caaccgaacc
     6841 catcgaaccg ccgataaagc ggaactcaaa cgcagcaacc acaacgggca gaccgttcat
     6901 cttgcctttc attaccacca aagcatcatc ttccccggtc agcttgcgtg ccgcactcaa
     6961 acgatccgga tattttttgc cgtctttaaa cttcaaagga tctgtcggtt tgacatttcc
     7021 ggcaacctcc tctctgccat cctcatccaa aagcagattc aggcgttgtc gtgccgacaa
     7081 cggattgtgg tggttgcatt tcggacaaac ctgattgttc tgctgcaact cggtcgaata
     7141 aacggttgcc gaacaagacg gacatttgtg ccacaaaccc tcgggaacat tggaagaacc
     7201 gtctttgctg cggtttttga ttttgggtgg caggatttta tctaaccagc tcatggacaa
     7261 ctcctaaaat atttagaatg cgtgacagaa tcagcgcacg gcatccttca actctttgac
     7321 caaagcaccg acgaccgcag cctcgttgcc cgcattgttt tcaatctctt tcacaatccg
     7381 gctgccgaca atgaccgcgt cggcaacccg gccgattttg cgcacgcttt ccgcattgct
     7441 gatgccgaaa ccgacaccga tgggaatatc gatatactga tgcaaatact ctattttacg
     7501 cgaaacctca tccgtatcca aacttgccgc gcccgttacg cccttgagcg aaacataata
     7561 gacaaatccg cctgccagct cggcaatggt tttaatacgg tcttccgtcg tcgtcggcgc
     7621 aatcaggaaa atacagtcga ccccgttatc cttcagctcg cgatagagcg gatcgatggt
     7681 ttcgacaggg gaatccaccg tcaacacgcc gtccacaccc gcctttgcgg cttcctgagc
     7741 aaactcccga taacccatct tatgtacagg gttcaaatat cccatcaaaa caaccggcgt
     7801 ttgcgtgtcg gtttcacgga attttctgac gacatccaag acatcgcgca gcgaaatccc
     7861 gtttgccaac gcccgctccg ccgcacgctg aataaccggc ccatccgcca tcggatcgga
     7921 aaacggcacg cccaactcca aaatatccgc accgtttgca accatgccgt gcatcaatgc
     7981 caaagttgtc cgaatatcgg ggtcgcccac cgtaatatag ggaatcaatg cctttccgcc
     8041 atccaaagcg gcaaaagcct gccggattct gctcatttgt ctcttccatc ctatcggcaa
     8101 atatgccata aaacacaatc agtaaatttt tcccagtata acatttgcgg tacggatagg
     8161 cgaatacggc aaacagaaac cacgcaaatc agagccgccg tttgaacgag gcggaaataa
     8221 acggcaagca tctcggatta tagtggatta acaaaaatca ggacaaggcg acgaagccgc
     8281 agacagtaca aatagtacgg agccgattca cttggtgctc cagcacctta gagaatcgtt
     8341 ctctttgagc taaggcgagg caacgccgta ctggtttttg ttaatccact ataacaaaat
     8401 tcagaatcaa gacagggcaa taccatactg gttttgcacc aacccgctat aaaattgcat
     8461 aaaaaaaata gacgctgtaa aacacagcgt ctatttttta tctggcggag taagagggat
     8521 tcgaaccctc gatgcaggtt gaccccacat gcttccttag cagggaagtg ccttcagcct
     8581 ctcagccatt actcctaagg aagctgtaac tataatcaac ttttccgata ccgtcaagga
     8641 aaaatggaaa gcaaatatta atcttttgaa ataaaataag atttaccctg attataccca
     8701 atcaggcact tcttttcttc ctgaatggaa atattcctga ctgccacatc ctcaagcaaa
     8761 atgcttttgt gcatcctgtt tgatttcctg aaaccacgcg tcttcaggaa aacgtgcaag
     8821 cgcgacatcc aatgttgaca gcaggttttg cgacagggct ttggtcaggt attcctcctc
     8881 gttggcttct gccgctttgt cgggctgttg tgccaaaccg tcggcacggg ctttgtgtcc
     8941 gtgtgccaaa tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta
     9001 cagatagtac gacaaggcga ggcaacgccg tactggttta aatttaatcc actataattg
     9061 agcgcggtaa ttttttgcgc ccaaagctgc ggtatgtcgg gattgtcttc aatcatgacg
     9121 gaaaggatgg atacggcggt cgccagcctg ccgccgttca ttctaaggtc gagggaacgg
     9181 ttgggcggca gggtgctgag tgtttctgcc attctgaacc aaaattcgcc actggcttcg
     9241 tcgggtgcgt tcaatttaag catttcatag tgaacctcct gatcatcgtt ttgcacgaga
     9301 ccccatactt cggcaaggat tttctgttct tcccggctca agtgttgcca cttttcaata
     9361 tgttgctgca tatcttttcc ctttgaacct gttttatacg gtctgaaata cagcgcgaac
     9421 cctatccaaa tcttcctgcg tatccacacc ggcggcgggg gcttctttgg cggtttcgac
     9481 ggcaatcggg tagccgtgcc acaggacgcg cagctgttcc agcgattcga tggtttccag
     9541 cggcgaaacg ctcatttcgg catagcgttg caggaagccg gcgcggtaag cgtagatgcc
     9601 gatatggcgc aggacggcgg tttcagacgg catttcgcgt tttcctgcac gcatcgcatc
     9661 gcgcggatag ggaatcgggg cgcggctgaa gtagatggcg ttgcggtttt tgtcgaggac
     9721 gactttgacg gcattgggat tcatcaattc gtcgaaatcg tgcaattcgt gtgccgcagt
     9781 tgccatctgt acgttgtttt ccaccaagac ttcggcagtg cggtcgatga gttcgggggc
     9841 aatcagcggc tcgtcgccct gtacgttgac aaccaccaga tgcggcggca gcttcagcgc
     9901 ggcagaggct tcggcaaggc gcgtcgtgcc gctttcgtgc cggtttgaag tcatgacgac
     9961 ttcgacgccg tgcgcctgac aggccgtctg aatatcggga tggtcggtgg caacgacgac
    10021 gcgcacggct ttactttttg ccgcctgttc ggcaacgcgc acgaccatcg gtttaccgtg
    10081 gatatccgcc aaggcttttc cgggcaggcg cgatgaatcc agccgcgccg gaatcaatac
    10141 gacgaattca gtcatgcttt gagttcctct tcgctcaacg ctcgcgcttc gttttccagc
    10201 atatagggaa tgccgtcttt aatcggatag gcaagcttcg cctgacggct ccacaattcc
    10261 tgtttgtcct gatgatattc cagcctgcct ttggtaacgg ggcagacgag gatgtctaag
    10321 aatttttttt ccatagggtt gctttcgggt ttgatggttc aatcaatcgg atgccctctt
    10381 caatatttca gacggctatc atttatttct gatgcctttt cccaacctgt ccggcttgtt
    10441 tacagcccgc atcgggattt ttaccgccgc agatgatgcg ccgcaactct tccgtaaacg
    10501 cgggcagatg atgtcgcgcg attttccctg acaatgcagt ccaagatact gctatttcac
    10561 tcatcgattt cttgattaag cgcggatttt tggcaacgac ggactgaccg acaggcgaac
    10621 cgtaaaaggc aatcatgccg tcaatttctt cttgcgtgta tatctcacgg gcattcttca
    10681 ataaagtatt gcggacagcc tgtttcactt cgggcgtaat caaatctttc aaaacattct
    10741 cacgatagcg gttaaaggct tttgccgcct gatctttttt cgcttccggc atttcggcaa
    10801 gggctttgtc cgcatacggt ttaaatccgg cattaaagcc ctcaatcata tttttttcta
    10861 tatcccggtc aaaattctgc gtatccagcc aacgcgccaa cgacgcgtcg ctgggcggag
    10921 cggcaaaagc attggtgcac aatgccagcg tggcgaaggg caataacaag gttttcagtt
    10981 taatattcaa tcctttaatg aatgattaat gcagataaaa aatacgaccg gttttctcat
    11041 tcctgtccaa gtggaaatcc ggagatttgg gatttctgaa gcctcctagg cattcctgaa
    11101 aattcgagac ttagattccc gcctgcgcgg gaatgacggc gcgaggcgtt cttattcaaa
    11161 tccgtaatca ctccgcccaa accgtgcttt cagacggcct tcggtacatc ttccaaccgc
    11221 tccaacacaa acgccgccaa atcaggttcg attatcgcac aaacgggcaa cacccaaaca
    11281 ttatcggtgc aaatgccgtc tgaaaatttg accgcatctt tctccgtaat aatgaccgcg
    11341 tccgcatcgg gcaaatctgc cgccgaaatg tcggcgtggt cgggcagcgc gacggttcgc
    11401 ttcacggtaa tgcccatatt ccgcaacgaa tcaaaaaacc gcgccggctt ggcaatacct
    11461 gccacggcga cgacggtttg atttttcaga cggcctgtgt ccagtatttc ggacggattg
    11521 ttcaaacggt aaatccgtcc cgcttcgata cggctgtgaa acatattttc agacggccta
    11581 aacagcgcat ccgccttgcc gccgctgacg acgaccgcat ccaccgaatc cagccgcaac
    11641 aaaggttcgc gcaaactgcc gttgggcagt aaatccaaat ccgtgcgccc cgtatccgcc
    11701 gccggaaaca ccgcgatttc cacatctcgc cgcagggcgt aatgctgcaa accgtcgtcc
    11761 gccacaatca gtccgatgtc gggatgcgcc gccagcaacg ccctgcctgc ctctgcacgg
    11821 ctgctgccca ccgccgtcgg cgcaacggtt ttgcggaaca gcagcaaagg ctcgtcgccc
    11881 gcatcttccg cccggctctt ctcattcaat acatgaaccg ccttgctctt gcgcccgtag
    11941 ccccggctga tgatgccgac cttgacacct ttttcctgca aacccgacac cagcgcggca
    12001 acaatcggcg tttttcctgt accgccggca tggatgttgc cgaccacgac cacaggcacg
    12061 ggcagctttt cgctttgccg ttttcccgat aaaaaatccg tccgccgttt tgccgcaatt
    12121 ttggcaaaca gcccggagag cggcttaagc agaaaagaca aaaccggata aggtttttgc
    12181 caatgccgtt cgataacggt atgaaatttg aaacaattcg gcatattcga tacaaatcct
    12241 cgaagcaaaa tcagcccggg tcattaatca tgcgtacccg atacagaccc acctataaac
    12301 aacgcgaaga ccaaaccgat aagcgaacca atcaggtaaa taagcaactg cgctttacag
    12361 aaattggcgc ggttcgggtt gcctctgccg aacacccaaa caaaaattga ccaaaggaat
    12421 catgaaaaca atcatcgtca acacccactg ccctgtcgtc atgacgggag gcggtttgtt
    12481 ttgaagtctc gggcatatcg ccaatatgat aacggacagg tttcatctct ttcattttcc
    12541 agtatctttc atttatagtg gattaacaaa aaccagtacg gcgttggctc gccttagctc
    12601 aaagagaacg attctctaag gtgctgaaac accaagtgaa tcggttctgt actatttgta
    12661 ctgtctgcgg cttcgtcgct ttgtcctgat ttttgttaat ccactatatc aatctgacag
    12721 caaaatgccg cctgaaaaca gttcagacgg cattttaaac cgcaaacggc ttacagcttc
    12781 cattcgctca gcttggcagc gtaagcttcc aaatctgcaa tcggacgggt tgccacgccg
    12841 ctttccatcg ctgctttggc ggcagccgta gcaacgcgcg gcagcaggcg ggaatcgaac
    12901 ggagtcggaa tcaggtattc cgcgccgaat tcaaatttct taccgtaagc ggcaaccact
    12961 tcttcggtta cctcttccat cgccaaatcc gccaaagcat acacgcaggc gcgtttcatt
    13021 tcttcgttga tggtcgtcgc gccgacatcc aacgcaccgc ggaagatgaa cgggaagcac
    13081 aatacattgt tcacttggtt cgggaagtcg gagcggccgg taccgataac cacgtccgga
    13141 cgggtttctt tcgccagcgg cggcaggatt tccggattcg ggttggccat agcgaacacg
    13201 atgggttttt cgttcatggt gttcagtatt tcaggcgtca gcaggttcgc gccggagagg
    13261 cccaagaaga tgtctttacc tttgacggca tcggcaagta cgcgctggcc attgtcttca
    13321 acggcgtaga attttttgga ttcgtccatg cggtctttgt cttcgcgggt ttggtaaatc
    13381 acgcctttgg agtcgcaaac ggtcacgttt tcgcgtttca agcccaaatc cagcaattgg
    13441 ttcaagcagg caatcgcggc cgcacctgcg ccggaacaca ccaaagtcgc ttcttcgatt
    13501 ttacggccgg taaaacgcag ggcgttcaat acggcggcgg cggtaatgat ggccgtgccg
    13561 tgctggtcgt cgtggaatac ggggattttg cagcgtttgc gtaattcgcg ttcgatgtag
    13621 aaacactcgg gtgctttgat gtcttcgagg ttgatgccgc cgaaggtcgg ctctaaagcg
    13681 gcgatgatgt ccacgagttt ttgcgggtct ttttcatcga tttcgatgtc gaacacgtcc
    13741 acaccggcga attttttgaa caatacgcct ttgccttcca tcacgggttt gcccgccagc
    13801 gcgccgatgt cgcccaagcc caaaacggcc gtaccgttgg aaatgacagc gaccaagttg
    13861 cctttggcgg tgtatttgta ggcattttgc ggatcggcat ggatttccat acaaggagcg
    13921 gctacgcccg gagagtacgc caacgccaaa tctttgtcgg tcgccagaga tttggtcggg
    13981 gtaacggaaa ttttgcccgg aacgggtaat tcgtggaact tgagggcggc ttctttcaat
    14041 gagttttcca tgtgtcaatc ctgttgcgag aaaaagattt gaatcggact gtccgaaacg
    14101 ctttcgggtc atcatgttac gggcggttcg ttaatattgc cgacgcatcc tgccttgtaa
    14161 ccgagcgtaa ttctatcatg ccgaaagttt ggcgcatacc gccattttca cctgcggcac
    14221 tgccgtttga ttttcggcgc gggcggatgc gcttgtttca atatttgccg catcaacagc
    14281 cgaaatgtct gtccaacact ttttctatgc tgtttctgac ttcatccaag ccgaggttac
    14341 tgtcgataac ggcgtaacgt tccggacagg cggcggcacg gttcagataa acgctgcgca
    14401 cacgcatgaa gaaatctgcc tgttcctgct cgaaccggtc tttctcgcgc gtctgcccga
    14461 tacgcgccat cgacacttcc agcggcacat ccaacagcag ggtcaaatcg gggcgcaaac
    14521 cgccctgcac ccaatgttcc aaaatttcaa tgtcttcaga cggcattccc cgcccgccgc
    14581 cctgataggc gaaggtcgca tcggtaaaac ggtcggacac gacatggatg ccgtctgaaa
    14641 gtgcaggcaa tatcacgtct tcaatatgct gcattctggc ggcgaacatc atcagtgttt
    14701 ccgcacgcaa accggctttg gtttcagggt tgagcaggat ttcgcgcaag gcctcaccga
    14761 ccggcgttcc gcccggttcg cgcgtgaaca gcacgggcag ccccctccgt tcaaaccatg
    14821 ccttgatgac ggcaaggttg gtggatttgc cggcaccgtc tatgccgtcc aaagtgatga
    14881 attgcggttt catgcttgca gttccggtaa gggtatgccg tccgaaaaca caaacttttc
    14941 agacggcatg gtttattttt tcaaaatata tttgcgaacg gcggcgttgt gttcggtcaa
    15001 atcatggctg aactggctca agcccgtacc gtccattttg gacacgaaat acaggtattt
    15061 ttcaccggac ggatgggcgg cggcatcgag tgccgccttg ccgggcagcg cgatcggggt
    15121 tggcggcaga ccgccgcgcg tgtaggtgtt gtacggcgtg tcgcggcgca ggtcggcttt
    15181 acggattttg cccttgtatg ccgcacccat gccgtaaatc acggacgggt cggtttgcag
    15241 gcgcataccg attttcaggc ggttgacgaa gacggaagcg acatggtcgc ggtcggcttc
    15301 atgccctgtt tccttttcga tcaggctcgc cataatcagc atttcataag gatttttata
    15361 aggcagcccg tcctgcctgc tttcccatgc ctcattcagg cggcgttgca tcgccttgta
    15421 ggcggtttgg taaatccgta aatcgctgcc gcccgcatcg atttcgtagc tgtcggggaa
    15481 aaactgccct tcaggattgc cgctgaaggc atcaggggca acttccgcca tcagtttttc
    15541 attgctccag cctttggtgt cgtgttcgat gtcgggcgtt gcgtcgatga ctttcctcat
    15601 atgcgaaaaa cgcgaacctt cgataatctg cacggtaacg gaatccggcc tgccgccgcg
    15661 cattttctgc aagatatccc aagcagacac ttccgaaggc agtctgtacg tccccgtatg
    15721 cagcctgttg tgcacaccca aaacgtaggc cgccgccgtc aaaacatgcc tgctgaacac
    15781 gatgcggtct tcggcaagtt tcctgccgac cgacgaaata ccctgatttt tggcaatctt
    15841 gattcggtat gccctgccat tatccttagg aacaaaaagc agcgcggcga aaacggctgc
    15901 cgacacggtc aaaaaaacgg cagaccattt caacaatttt ctcaacatgg taggattccc
    15961 agcattcgga atacgtctta aaattcgggc gacagtataa cccaaattgc ccgccggaca
    16021 ggaagaacat catggacaat cacgccgaag cacactggca aaacggctgg cttcaaagca
    16081 tacgccatac cccgtcgccc aatttcagcc cgagggaaac gggggaaacg gtttccctga
    16141 tcgtgttgca caacatttca ctgccgccgt tcgaatacgg cacggatgct gtggaaaagc
    16201 tgtttgccaa ccggctcgac cccaacggac atccgttctt cagcctgata cacactttgc
    16261 gcgtatccag ccatttctta atcaaacgcg acggagaaac ggtacagttc gtttcctgcg
    16321 acgacatggc gtaccacgcc ggcgtgtctt cattcggcgg cagggaaaaa tgcaacgcat
    16381 tttccatcgg catcgaactg gaaggctgcg atttcgaacc ctttaccgaa gcgcaatacc
    16441 gttcgctcga agccttgttg gacgcaatct gccgccgcta ccccatcacg gcggtaacgg
    16501 ggcaccaaga catcgcgccc ggccgcaaaa ccgaccccgg tcattttttc gactggcggc
    16561 ggatacgggg gaaagggttt cccgtggaca gaaatgccgt ctgaaaacca cagattttag
    16621 acggcatttc ggaaaatttc cgcttatttc gacacactgc tgtcagaccc tgcaattttc
    16681 gttataattc aaacgttata accggccgtt ccgtcagact gccggaaacg caagcccgat
    16741 ttaaccgcag tacccgcact ttgcgcatcc tgcatcccga aatttaaagt gattgccatg
    16801 atttacatcg tactgttcct cgccgccgtc ctcgccgttg tcgcctacaa tatgtatcag
    16861 gaaaaccaat accgcaaaaa agtgcgcgac cagttcgggc actccgacaa agatgccctg
    16921 ctcaacagca aaaccagcca tgtccgcgac ggcaaaccgt ccggcgggcc agtcatgatg
    16981 ccgaaacccc aaccggcggt caaaaaaacg gcaaaacccc aagaccccgc catgcgcaac
    17041 ctgcaagaac aggatgccgt ctacatcgcc aagcagaaac aggcaaaagc ctccccgttc
    17101 aaaaccgaaa tcgaaaccgc cttggaagaa agcggcatta tcggcaactc cgcccacacc
    17161 gtttccgaac cccaaaccgg acattccgca ccgaaacctg ccgacgcgcc ggcaaaaccc
    17221 gttcccgttc cgcaaacgcc ggcaaaaccg ctgattacgc tcaaagagct gtcgaaggtc
    17281 gagctgccct ggtttgacgt gcgcttcgac ttcatctctt atatcgcgct gaccgaagcc
    17341 aaagaacttc acgcactgcc gcgcctttcc aaccgctgcc gctaccagat tgtcggctgc
    17401 accatggacg accatttcca gattgccgaa cccatcccgg gcatccgcta tcaggcattt
    17461 atcgtgggta ttcaggcagt cagccgcaac ggacttgcct cgcaggaaga actctccgca
    17521 ttcaaccgcc aggtggacgc attcgcacaa agcatgggcg gtcagacgct gcacaccgac
    17581 cttgccgcct ttatcgaagt ggcttccgca ctggacgcat tctgcgcgcg cgtcgaccag
    17641 accatcgcca tccatttggt ttccccgacc agcatcagcg gcgtagaact gcgttccgcc
    17701 gtaacgggcg tgggtttcgt tttggaagac gacggcgcgt tccactatac cgacacgtcg
    17761 ggctcgacca tgttctccat ctgctcgctc aacaacgagc cgtttaccaa tgcccttttg
    17821 gacaaccagt cctacaaagg cttcagtatg ctgctcgaca tcccgcactc tccggcaggc
    17881 gaaaaaacct tcgacgattt gtttatggat ttggcggtac gcctgtccgg ccagttgaac
    17941 ctgaatctgg tcaacgacaa aatggaagaa gtttcgaccc aatggctcaa agacgtgcgc
    18001 acttatgtat tggctcgtca gtccgagatg ctcaaagtcg gtatcgaacc gggcggcaaa
    18061 accgcattgc gcctgttctc ctaaaatcga atgcaatcaa agcggatatg tcgttacggc
    18121 atatccgctt tttttcgggt aaaattcagc tgttttcaac ctatcatttt tcagacgacc
    18181 tttgcccgtt tcatcccgta tcagacaaag gtcgtctgaa aaccttctct catgaatcca
    18241 aactcaaaac ataacactaa ctttaccaat cttcttaaac cgtctgatag tgatataaaa
    18301 caatttgccg cacaacacat ccgccacctc accgacctgc tcaaccgcta tgcctacgaa
    18361 tactacaccc tcgacgcgcc cagcgtaccc gatgccgaat acgacaaatt attccgcgag
    18421 ctcgaagcgt tggaattaaa ccatcccgag ctcaaactgc ccgacagtcc gacccagcgc
    18481 gtcggcggcg agcctttagc gggatttgcc gaagtgcgcc acgaagtgcc gatgctgtcg
    18541 ctgaccaacg ctttttctcc gcaagatgaa aacggcgtgt tcgaccatgc cgaaatgtac
    18601 gctttcgacc aacgcgtgcg cgacggtttg gacggcggca aacccgaata cgttatcgaa
    18661 cccaaattcg acggcctcgc catcagcctg ctctaccgcg acggcgtatt ggtacaggcg
    18721 gcaacgcgcg gcgacggcac aacgggcgaa gacgttaccc aaaacatcaa aaccgtcgcc
    18781 aacatccccc tgcggctgca cggcgaaaat acgcccgaac tcatcgaagt acgcggcgaa
    18841 gtactgatgc aaaaagccga ttttgccgcc ctcaataaaa gacaagccga aaacgggcaa
    18901 aaaccctttg ccaatccgcg caacgccgct gccggcagcc tgcgccaact cgattcgcgc
    18961 atcactgcgc aacgcaaact gcactttttc ccctactccg tcgcccgcca gcaaggcggt
    19021 ttcgtcgcgg aagaacacat ccaagaactc gcctatttcc aagcactcgg cttcagtctg
    19081 ccaaatggca atttcggctg tttcaaaaat atcgacgaag tattggcgtt ttacgaacaa
    19141 atgcagcaaa aacgccccga actgccctac gaaatcgacg gtatggtggt caaagtcaac
    19201 agcttggcgc aacagcgcga actcggcttc atctcacgcg cgccacgctg ggcgattgcc
    19261 cacaaattcc ctgccgaaga agccctgacc attgtcgaag cgattgacgt gcaaatcggc
    19321 aggacgggcg cggtaacgcc ggttgcccga ttacaacccg tattcgtcgg cggcgtaacc
    19381 gttaccaacg ccaccctgca caatcaggac gaagtatcgc gtaaagacgt gcgcgtcggc
    19441 gataccgtcg tcgtgcgccg cgccggagac gtgattcccg aagttgtgcg cgtgattttt
    19501 gaacgccgcc cgatgcggga aaccgccgtt gccgtttcag acggcatcgg gcatcggcag
    19561 gacgatttgt ttgccgaaac accgtccgct aatcaaaccc aatccgttcc gctccacaag
    19621 ccctaccgcc tgccgaccca ttgccccatc tgccgcagcg aaatcgaacg cgaagaaggc
    19681 gaagccgtcg cccgatgcag cggcggcatg ctttgtcagg cacaacgcgc gcaaggctta
    19741 atccacttcg cctcgcgcaa agcgatggac atcgacggct tgggtgaaaa acaaatcgaa
    19801 cagcttgtcg cccaagacct cgtccgccac ttcgccgacc tctaccgcct cgatattccg
    19861 accctgcaaa aaatgaagga agcggcggac aaaaccgttg ccgaatcgga tcaaatgccg
    19921 tctgaaggca gttcggtcgg tgcgtcgggc aaacataaaa aacaaccgac caagtgggcg
    19981 gaaaacatcc tcgcaggcat agaatccggc aaaacgcccg aactcgcgcg cttcctgttc
    20041 gcgctcggca tccgccacgt cggcgaacgc accgccaaaa cgctggcaca ggcattcggc
    20101 acattggaac gcgtccgccg cgcccccgaa cccgtcctcg cctgcctgcc cgacatcggt
    20161 acggtagtgg cgcgttccat tgcccacttc ttcgcccaag ccgaacagca ggcgatgata
    20221 gacgagctgc tcgccgcagg cgttgccccg caagcccaag ccgtcagcct gcctgccgca
    20281 caatatgccg aaccgcaacg ctggattgcc cgcctgcccg gtttcaaaat tagcgaaaac
    20341 aaagcccaag ccttatggga actcgccggc aaaagtatag agggtctgca aaccgacaaa
    20401 tccctccccg ccgactggca agcatggcgc agcgaaccac aaaacgccgc cctactcgaa
    20461 aacctgaaaa ccttcttcgc gcaaatgccg tctgaagacg aggcggcgca aggttcagac
    20521 ggcatcaata aagccgtagc gggcaaaacc ttcgtgttaa ctggcacact gcccaccctc
    20581 aaacgcgacc aagcccaatc cctaatcgaa gccgcaggcg gcaaagtttc cggcagcgtg
    20641 tccaaaaaaa ccgattacgt cgtcgccgga gaagccgcgg gcagcaagct ggaaaaagcc
    20701 aatgccttgg gcgtttccgt cctcagcgaa gccgaactgc ttaccctgct cggctgaacc
    20761 catgccgtct gaaccgcctt cagacggcat tgcccacttt tccgaaccac accatgcaca
    20821 ccatttcctt tccaaaccgc acccgactca ccgccctgcc gcccctgtcg ctttacatcc
    20881 acatcccgtg gtgcatcaaa aaatgcccgt attgcgactt taattcccac agcctgaaaa
    20941 acggattgcc cgaagccgcc tatatcgacg cgctgctgac cgacttgcag ctcgaattgc
    21001 ccaatatttg gggcaggccg gtggaaacga tctttttcgg cggcggtacg cccagcctgt
    21061 ttcaggcgga atcgattgac cgtttattga gcggcgtgcg ttcgctgttg cgcttgcagc
    21121 cggaagcgga aattaccttg gaagccaatc cggggacatt tgaaatcgag aagtttcaag
    21181 gatttaaaga cgcaggcatc acgcggcttt ctattggtgt acaaagcttc aacgacgata
    21241 tgctctcaag gctggggcgc gtccacaacg gtaaagaagc cttaaccgcc atagctactg
    21301 ccttgaaatt atttgataaa atcaatatcg acttgatgta cgccctgccg aaccaaaccg
    21361 ttcggacggc attggatgac gtacaaaccg ccatcgccac gggcgcaacc cacatcagcg
    21421 cgtatcacct gacgatggaa ccgaacacgc cgttcggaca tacgccgccg aaaggtttgc
    21481 cgcaagacga agccgccctc gacatcgaag acacggtaca cggcgcattg gaaaacgccg
    21541 gctttataca ttacgaaaca tcggcttttg cgaaacccgc catgcggtgc cgccacaatt
    21601 tgaactactg gcagttcggc gattatttgg gcatcggcgc gggcgcgcac ggcaaaatct
    21661 cctatcccga ccgcatcgag cgcaccgtcc gccgccgcca ccccaacgac tacctcgcct
    21721 taatgcaaag ccaaccgagt gaagccgtcg aacgcaaaac cgttgccgcc gaagatttgc
    21781 cgttcgaatt catgatgaac gccctgcgcc tgaccgacgg cgtacccacc gcgatgttgc
    21841 aggagcgcac gggcgtaccg agtgccaaaa tcatggcgca aatcgaaacg gcaaggcaaa
    21901 aaggcctgct ggaaaccgac cccgccgtat tccgcccgac cgaaaaagga cgcttgtttt
    21961 taaacgattt gctgcagtgt tttttatagt ggattaacaa aaaccagtac ggcgttgcct
    22021 cgccttagct caaagagaac gattctctaa tgtgctgaag caccaagtga atcggttccg
    22081 tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatat
    22141 aagcgcaaac aaatcggcgg ccgcccggga aaacccgccc cgaacgcgtc cggaaaatat
    22201 gcttatcgat ggaaaacgca gccgcatccc ccgccgggcg tttcagacgg cacagccgcc
    22261 gccggaaatg tccgacgctt aaggcacaga cgcacacaaa aaaccgtatg cctgcacctg
    22321 caacaatccg acagataccg ctgttttttc caaaccgttt gcaagtttca cccatccgcc
    22381 gcgtgatgcc gccaccacca tttaaaggca acgcgcgggt taacggcttt gccgtcggca
    22441 aagcagccgg ataccgctac gtatcttgaa gtattgaaaa tattacgatg caaaaagaaa
    22501 atttaagtat aatacagcag gattctttaa cggattctta acaatttttc taactgacca
    22561 taaaggaacc aaaatatgaa aaaagcactt gccacactga ttgccctcgc tctcccggcc
    22621 gccgcactgg cggaaggcgc atccggcttt tacgtccaag ccgatgccgc acacgcaaaa
    22681 gcctcaagct ctttaggttc tgccaaaggc ttcagcccgc gcatctccgc aggctaccgc
    22741 atcaacgacc tccgcttcgc cgtcgattac acgcgctaca aaaactataa agccccatcc
    22801 accgatttca aactttacag catcggcgcg tccgccattt acgacttcga cacccaatcg
    22861 cccgtcaaac cgtatctcgg cgcgcgcttg agcctcaacc gcgcctccgt cgacttgggc
    22921 ggcagcgaca gcttcagcca aacctccatc ggcctcggcg tattgacggg cgtaagctat
    22981 gccgttaccc cgaatgtcga tttggatgcc ggctaccgct acaactacat cggcaaagtc
    23041 aacactgtca aaaacgtccg ttccggcgaa ctgtccgtcg gcgtgcgcgt caaattctga
    23101 tatgcgcctt attctgcaaa ccgccgagcc ttcggcggtt ttgttttctg ccaccgcaac
    23161 tacacaagcc ggcggttttg tacgataatc ccgaatgctg cggcttctgc cgccctattt
    23221 tttgaggaat ccgaaatgtc caaaaccatc atccacaccg acaaagcccc cgccgccatc
    23281 ggcgcgtaca gccaagccgt ccgagcaggc gacaccgttt acatgagcgg tcaaatcccc
    23341 ctcgatcccg ccacgatgac cgtcgtcggc aacggcgatt tccgcgccga agcgcgccaa
    23401 gtgttccaaa acctgcaagc cgtcgccgaa gcggcaggcg gcacgctggc cgacatcgtc
    23461 aaactcaacg cctacctgac cgacttgggc aattttgccg tcttcaacga agtcatggcg
    23521 gaatttatcg ccgagccctt ccccgcccgc gccgccgtcg gcgttgcctc gctgcccaaa
    23581 ggcgtgcagg tcgaagccga agccgtcctc gttttgaacg cataattcca cgttcggact
    23641 gcatgatatt tcaaaaccat gccgtccgaa ccgcaacact ttcccacttc acaacatctg
    23701 caatggcaca ttacgcaatc ggcgacatac aaggctgttt cgacgaactg accgcgctgc
    23761 tcggcaaaat cggtttcaac cacggcacgg acaccctctg gctgacgggc gacatcgtca
    23821 accgcggccc gaaatccctc gaaacgctgc aattctgcat ccggcacgaa aacagcgtgc
    23881 aaatcgtcct cggcaaccac gacctgcacc tgctcgctgt cggctgcggc gaaggcgcgc
    23941 ccaaacgcag cgacacaatc gaacccatac tcaaacaccc cgacggaaaa aaaatgctcg
    24001 actggctgcg tgcgcaaccg ctgttgatac gcgagggcag ccgcgtgatg gtacacgccg
    24061 gcatcctgcc gcaatggcgc ataaccaaag ccgaatcgct cgctagagaa gccgaagccg
    24121 aactgcgcgg caaaaaatac gtcaaattct tctccaaaat gtacggcaac aaaccggccg
    24181 cgtgggacga aggtttgaaa ggctatgccc gcctgcgctt catcgtcaac gccttcacgc
    24241 ggatgcgcgc cctgaccttt aaaaacgaac tggatttcga ctacaaatcc acagtgaaaa
    24301 aaatgccgcc ttacctgcgc ccgtggttca aagcccccga ccggcaaaac ctcgaccgca
    24361 ccatcatctt cggacactgg tcctcgctgg gctacacgaa tgccgacaac gtcatctcgc
    24421 tggacaccgg cgcgctgtgg ggcggacagc tgaccgccgt caacctcgaa acggaagaaa
    24481 ttacccaagt ccaagccgcc ggcggcatag actggaaaag cttcgcaaaa taacggaccg
    24541 ccccactatg cacaaaatcc tgcccatcgc ccacgtcctc tcccgactgg gtatgctgtt
    24601 ttcctttatc ctgctgatac ccgccgccct ctcctacgcc ttttcggacg gcgcgtacac
    24661 cgctttcgcc accaccgcga ccgttaccct ttccggctcg tgcatcgtcc ggctcgccac
    24721 cctccggttc aggcgcgaac tgcgcccgcg cgacggcttt accctcgtcc tgatgttgtg
    24781 gctggcgttt gccgctatgg ctgcgatgcc gatgtacctg tatttcccga atatgggctt
    24841 taccgacgca ttttttgaat cgatgtcggg actgaccacc accggcgcga ccgtcatccc
    24901 ccacgtcgac gggctcgccc cctccgtcaa cttttggcgg catatgctca actggctggg
    24961 cgggatgggc atcatcgtcc ttgccgtcgc catcctgcct atgctcggcg taggcggcac
    25021 gcagttattc aaagccgaaa tccccggcat tgacaaagaa agcaaaatgt cgccgcgcat
    25081 ttcccaagtg gcgaaaaaac tttggttcgg ctacaccctg atcaccatcc tcgcggcagc
    25141 ctgcctgcat tttgccggga tgggctggtt cgatgccgtc tgtcacgcga tggctaccct
    25201 ctcgctgggc gggttttcca cccacgatgc cagcatagct tattacaact cccccctcat
    25261 cgaagcggtc atcatcgttt tcaccattgt cggcggcatc aattttgcca accatttcgc
    25321 cgccctcaac agccgcagcc tcaaaaccta ttggaaagac gaagaatgcc ggacgatgct
    25381 gctgctgctc tccggcagca tccttgccgc cgccctgtac ctgtggcaca ccggctatta
    25441 cgccggtttt accgaatccc tgcgctacac cgccttcaac ttcgtctcca tcggactggc
    25501 aaacgggctg gccaacaccg acttcgcaca atggccgctc ctgatttccc tgtggatgtt
    25561 tttcctcgcc aacatcctcg ccaactccgg ctcgaccggc ggcggcatca aaaccatacg
    25621 cgccctcgtc ctgttcaaat tcagcctgcg cgaaatgatg gtgctgctgc accccaaagc
    25681 cgtccgcacc gtcaaaatca gcggcaaggc catacccgac cgcctcgcgc tgaccgttat
    25741 gtccttcatc ttcatctact ttatgaccgt cgtcctcttc agcttcctgc tgatggcgag
    25801 cggtatggaa tttaccaccg ccttcaccgc cgtcatcgcc tgcatcacca acgccggccc
    25861 cggcctgggc gaagtcgggc ccgccggcaa ttacgccggt ttggacgtga tgcagaaatg
    25921 gatctgcgtt accgccatgc tcttgggcag gctggaaatc ttcaccgtct tcatcctctt
    25981 cacccccgcc tactggaaga aataaaaaat gccgtctgaa aacttttcag acggcatctt
    26041 gtcctttaaa gaagcggaag ctctacaaaa ggaaagaaat gctgaaacct ctgataaatt
    26101 tcaggatatt tctctaaggc ttttaatgct ttttcttttg aaataggcgg atcatagaca
    26161 tctatccccc ttaagaaggc aatgcccgtt aatgcatttt tttgatcatt tgaaatgtaa
    26221 ccataattaa cgactccaat tacatcataa atttccccta tactcttatc aaaatcaatc
    26281 aaatcaaaaa tatacaatgg aattgtatca ataggagtat ctctgacaac tatatccaac
    26341 cataatttaa attcagaaag attaatagca tcatagaaca tacagactat tgcaaatcct
    26401 aaatcttcac aattttcttt actaattttc cacataatta tttaccttta tccattgctt
    26461 cccaaccgat acatccggta ttttagcttc tccttttccg tcaaacactc ctttggttac
    26521 ctgctttgtg tcaaaaatgc cgtctgaaag ccgaatatca tttcagacgg cattttgact
    26581 gtttaaagcg ggggcagttc tacaaacgga aagaaatgct gaaacttctg ataaatttca
    26641 ggatgtttct ttaaggcttt taatgctttt tcttttgaaa taggcggatc atagacatct
    26701 atccccctta agaaggcaat gcctgtcaag gcattttttt tcgatttcgg caggctgtaa
    26761 ctcgaaacaa aatctacaat attgtcaatg tctccaattc ccccatcgaa atccgccaag
    26821 tcaaaaatat aaaaagggat gtcctcgatg ggcatatctc gtattacttg ttcaatccat
    26881 aatttgaatt catttaaatc aatagattga gagaataagc atttaattgc aaattctaaa
    26941 tcatcactat cctcttttat gattttccac ataattatct tcctttgttc atcattatct
    27001 tcctttgccg tcaaacactc ttttggttac ctgctttgta tcaaaaaatg ccgtctgaaa
    27061 gccgaatatt gtttcagacg gcattttgac tgtttaaagc ggaggcagtt ctacaaatgg
    27121 aaagaaatgc tgaaacttct gataaatttc aggatatttc tctaaggctt ttaatgcttt
    27181 ttcttttgaa acgggaggat catagacatc tatacccctt aagaaggcaa tgccggtcaa
    27241 ggcatttttt ttcgatttcg gtaggctgga actcggagca aaacctacaa tattgccaat
    27301 atcgccaatt tccacatcga aatccgccaa atcaaaaata taaaaaggga tgtcctcgat
    27361 gggcatatcg cgtattactt gttcaatcca taacttgaat tcatttaaat caatagattg
    27421 agagaataag catttaattg caaatcctaa atcatcacta tcctctttta tgattttcca
    27481 cataattatc ttcctttgcc gtcaaacgct cttttagtta cccgctttat atcaaaaata
    27541 ccatctgaaa gccgaatatc gtttcagacg gcattttgac tgtttaaagc gggggcagtt
    27601 ctacaaacgg aaagaaatgc cgaaacttct gataaatttc aggatgtttt tttaaggctt
    27661 ttaacgcctt ctccctagta atcggaatat cgtaaaaccc ttgattccgt atataggtaa
    27721 tgccatatac ggcatctttt tcctttttcg tcataccggt agttggaaca aagccaataa
    27781 tattatatat atttatggaa caatcatctg attccaaaca atctattaaa tcaatcagat
    27841 accttggaaa actttcggca gggacagtcc tgacaattcc atataaccaa tccaagaatt
    27901 cttttttgtc ggctgcattg cgctcataac acaatatagc aaatcccaaa tcataacaat
    27961 tgtctcgtgt aagccccaac ataatcattt tcctttatcc attgcttcac cacctatatg
    28021 ccctgtttgc gtatgctttc tttcggatat taactgcatt cgtcccgtat cctgatgatg
    28081 atgccaagta tagctcggta ttttagcttc tccttttccg tcaaacactc ctttggttac
    28141 ctgctttgtg tcaaaaatac cgtctgaaag ccgaatattg tttcagacgg cattttgatt
    28201 gttgggtttt taatcagagc tttgtttgat aatttgttat tttgaataaa atacaggttc
    28261 tgtcgagctg ataacttcta ttacagaatc gctaaaaaag aaaatataag ccttataata
    28321 tttcttttca aaaaaatcaa aactttcttc tttcaaccag tccatataac tgtcatctat
    28381 tatctcgaaa aaataatttg cagtaggatt cccatctgaa ttattttctt ctttattttc
    28441 aaataaatat tctgaaaatt ttactttata agattcatct gttatttgaa aatgaattat
    28501 attgtcaata aatatttcaa tatttaattc ataatttcgg tcggatgcca aaatgttcac
    28561 atcttgttcg gtaacacaaa ttttgtctac gtaaaatctg tgattattgt tagaaaattg
    28621 taatggttta atcttcacta tcgttttcca tatctgattt ttgttttctt attgccctgc
    28681 cggataaagc aggtctgcct ccgtactgtc tatcctcaca tttactttcc tgccatcggc
    28741 ttttatctcc ccggttttac cgtttttgcc aacttcgcgg ctttggcggc ggcaatgttg
    28801 aaggcggctt cgacggtttc ggcggcattg gggttgaccc gtactcatcc gcaaacggca
    28861 acacgacgga cggtgtttgc ttttcagccg tctgccgttt tgaccaaggt gctgccgtgg
    28921 aaggagcaag tgtaggattt ttgaagaact cagttctcaa tatcctattt caaacgaagg
    28981 aaatgccgtc tgaaaattaa acacggtttc agacggcatt ttgattgttg ggttttcaac
    29041 ccgacctacg gttcctaatg attttcagat aaaaataaag aaatatcttc taacgcatct
    29101 tcagccctac cttgaagctc tggatcatct tgctttgaat gtagtaaaga aataacttta
    29161 tcgtaatcac cgatatttga atgtagcctg gcgagatgtc cgaaacaggt tagcgataat
    29221 ccggcaatat ccaagtcttt atttttcgat aattccatca atttctcaga aatccacggt
    29281 acttcatatg ctttggaatt aaataccata tcaagaatgc catctataac actttgttta
    29341 tcatcagaag aaatcaactt ttctgtttta cttttatcag tcatttttta atttttcctt
    29401 ttgaattaac taatccaatc ctgctttttt taaagcattt ttctgatcgg tatgcaaatc
    29461 ttgccaactt ctgacatgcc cgtgatattc cgcaaaaccg ttattaaaag tttgagtttt
    29521 gtctaataca acgatttcat tattggcttt atcaacccca actcttcgag gagaagttga
    29581 tttaacttga acggaattat caagagctgc ttgtccgttt gttggtgctc tacttttcac
    29641 agaattatca tttttgccat gataagaagc gtctttatat cgttggttgc ctttatcata
    29701 cggacaatca ttatgaaccc aaaccccttc cgtttccgcc tgactgccct tgacgaagta
    29761 agtatgccag tcggcaacgg tcagattgta ggcttgcagc ggttccggtt tgacggtaat
    29821 gctttgaacg gtctgctccg caccgttttc ggcaaatagc ctgcttcccg ctttcaaatc
    29881 ttccgcttta atccatttgc cgtccgaata aaacgggtgg atgcggttgg aaatcagggt
    29941 ttgactgttg tcgaggccgt ctgaaacttc aatgtaaacg gtttcttgat acggattgcc
    30001 gtatcgggcg gtaacaggtt tgtatcccgt ttctccgctt gcctcgtcct tggaaaggac
    30061 gcggtcgccg gcttgaatat gggcaatggc ttttcagccg tctgccgttt tgaccaaggt
    30121 gctgccgtgg aaggagcagg tgtaggattt ttgaagaact cagttctcaa tatcctgctt
    30181 caaatgaagg aaagaccgtc tgaaaaacaa gcttaatttc agacgacctt taatcaaatt
    30241 taagaaagcc cttaccaagc aaacggtttc cgcttcatat ccgaaaggtt gtcaacttca
    30301 ttattcagca agaactgctc aaaagcattc caacctttct tttccaccaa ttcggcttcc
    30361 tgtttataca aggggacaag caaagggaaa acgatattgt agtgttcgcc ataacagacc
    30421 tgaaaatcat cgtcgaaata aaatggggcg gaaacataca gtgcatccat ttttgttgaa
    30481 tcaacaatgc ttctgggcaa ataaacaacc tgcccccgaa gaaggatttt tttgttctca
    30541 atgatggttt ctgccaacca tcttaaaaat gggaaaatct gattttcatc atattttaaa
    30601 gaacatacga aaagtatttc aaatctgctt ttataattca gatcatgttt gcttaaccct
    30661 aaagtaatat aagtttttaa atccctgtta ggggaagagg gaatagctgc gatatttaat
    30721 ttgtactgac ttcccgaatc gcttttcgag ccatgagaaa tagtgcctat atatttttca
    30781 atatgattaa tcatattttt cttagcttcc catcactgta tcgaataatc atagaccaga
    30841 ttgagacaaa acgccttgtt cccattgata gaaagtccgt ctgaaaagta aaagtgtttc
    30901 agacggacta cgtttgccga ctatccgacc cactttatta tccaatgaga ttcttgcagg
    30961 tattctttta atatttctaa gttttcgacc cagtcatcgt aactgtcaaa tgatgagaat
    31021 ttgtcatttg aagataacag tatcagaaat ccaccggtat tttcctggtc atcaataatt
    31081 tttatgaatt tttcttcagg attgacccca ttcaggataa cccccaaagt attgaatttt
    31141 atgtccatag acacacccta atctttaatg ggaatatggt tttttcaacc caacctacac
    31201 tttttattag ttcccatacg aaccaaaacc ttcataatta caatcatttt tttgtgcaat
    31261 accaaataac gtagtttcaa tagctaaaat gttttcaagg gaagggacga tattgacgga
    31321 acaatagcaa gtccacattt ctccgaattc atcttgttca atactgattt gaaaatcttg
    31381 ccgataagaa atgagttggc tttctattga ttgcagattt ttttgagaac ctcctataaa
    31441 aaactctatt atcataggtt ttgtcaaatc aaaccctagt tcttgatatt gtttcagtac
    31501 atctgaagta gatttttcgt caacaatcat ttttaatctc catcttgttt tcggtatttt
    31561 ttccccgggc tttcaattcg attgtaatta ttggtacttt tggaaccacc gtgagcgtca
    31621 attcttctag attcatcttt gaatgcctct cgattatttt tggcaggagt aaaatctgta
    31681 gctatatgag ggtcattatt aaccctagct tctcttgctc cagagatttg ggaacgttgt
    31741 cttgtgccct ttccagaata agttcgacca ctttcatgag tattagtata agaaccaact
    31801 tctgtcctat atttttctcc atacggacaa tcattatgaa cccaaactcc ttccgtttcc
    31861 gcctgattgc ccttgacaaa gtaagtatgc caatcggcaa cggtcagatt gtaggctttg
    31921 agcggctgct gtttgagggt aatgttttga accgtctgtt ttgcaccgct ttcggaaagc
    31981 agggtgtcgc cttttttcag acgacctgcc tgtatccatt ttccttgact gtaaaacggg
    32041 tggattttat tggaaatcag ggtttggttg ttgccgatgc cgtctgaaat ttcaatgtaa
    32101 acggtttctt gatacggatt gccgtatcgg gcggtaacgg gtttgtatcc cgtttttccg
    32161 cttgcctcgt ccttggcgaa gacgcggtcg ccggttcgga tacgggcaat ggctttgtag
    32221 ccgtctgccg ttttgaccaa ggtgctgccg tggaaggagc aggtgtagga tttttgaaga
    32281 actcagttct caatatccta tttcaaatga aggaaatgcc gtctgaaaat taaacacggt
    32341 ttcagaagac attgtgattg ttggtttttt aacctacgct tgcgaaatta attcctttct
    32401 cctgaatact gcaccattta tttggtttaa ctccatagaa gaatgattag aataaataca
    32461 ttcaaagtca gcttcatcaa aatattctcc acacatagac cataactctg taatcaaata
    32521 ttccatatct tcatggtctt ctgtgatggc ttctcttgat aaataatttt tgatttttaa
    32581 aaaccttctt ccttcctcgt aatctacggt aattaacctg atatctgagt gtatcatgcc
    32641 cagcattgct atattcatac cgactcgcag ccagcttggt aatatattca taactattgc
    32701 acctttttaa tggttttagg gttagccgga acaatgtgaa ctccattttt acctgaatga
    32761 atcgtaccaa aattggtact taatcctgat ttcccatcgc tgcctatagg atacccaaaa
    32821 tcaacaacag gatttcctct tgctccttta gaaataactg gataagcacc tgaatgtatt
    32881 ccgttcaaca attcttgagg attaatgttt tgatttaaag tactcttacc ttcaatgtag
    32941 tttccatgtc ctgaaatatg tttcccttga gctccatcat gaattttagt cccaatagat
    33001 tttgctttaa aatcaacatt gcgggtttga aacatttctc catctacaga ctgagataat
    33061 cttgaagcag tgttatgctt gtaggagtct gagaaatccc cgctaaccgc agccttcccc
    33121 ggttttgccg cctttgtcag gtttttgact ttggctgctg cggcaacgtt gaagacggct
    33181 tcgacggttt cggcggcatt ggggttttcc tgtatccacc ggtcaacggc ttcgcgcgta
    33241 ttcttttcaa agcccgccac gctgcccaag ccgccgatga cggcgaattt gccctcggcg
    33301 gg
//
LOCUS       DAWFLT010000014        33188 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWFLT010000014 DAWFLT010000000
VERSION     DAWFLT010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33188
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.014"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(98..844)
                     /locus_tag="WGR74_000658"
     CDS             complement(98..844)
                     /locus_tag="WGR74_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0905131.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(1023..1694)
                     /locus_tag="WGR74_000659"
     CDS             complement(1023..1694)
                     /locus_tag="WGR74_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ0905132.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1763..2434)
                     /gene="rpiA"
                     /locus_tag="WGR74_000660"
     CDS             complement(1763..2434)
                     /gene="rpiA"
                     /locus_tag="WGR74_000660"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ0905133.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2511..2993)
                     /locus_tag="WGR74_000661"
     CDS             complement(2511..2993)
                     /locus_tag="WGR74_000661"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ0905134.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(3025..3714)
                     /locus_tag="WGR74_000662"
     CDS             complement(3025..3714)
                     /locus_tag="WGR74_000662"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ0905135.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLEIGLASAD
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAISIADTLKRAEGGQISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIRPLLVQGDARNLKLTQPQD
                     AYIVRLLLNAV"
     gene            complement(3711..4445)
                     /gene="dnaQ"
                     /locus_tag="WGR74_000663"
     CDS             complement(3711..4445)
                     /gene="dnaQ"
                     /locus_tag="WGR74_000663"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ0905136.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGAAAEEKMETKPVVHTETKRSGKLKVIRADENELAAHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            complement(4601..5911)
                     /locus_tag="WGR74_000664"
     CDS             complement(4601..5911)
                     /locus_tag="WGR74_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0905137.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5901..6086)
                     /gene="ccoS"
                     /locus_tag="WGR74_000665"
     CDS             complement(5901..6086)
                     /gene="ccoS"
                     /locus_tag="WGR74_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ0905138.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6086..6385)
                     /locus_tag="WGR74_000666"
     CDS             complement(6086..6385)
                     /locus_tag="WGR74_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905139.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6736..7932
                     /locus_tag="WGR74_000667"
     CDS             6736..7932
                     /locus_tag="WGR74_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0905140.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTAGSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            complement(8568..10409)
                     /locus_tag="WGR74_000668"
     CDS             complement(8568..10409)
                     /locus_tag="WGR74_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ0905141.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFKIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGSILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            10500..10970
                     /locus_tag="WGR74_000669"
     CDS             10500..10970
                     /locus_tag="WGR74_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ0905142.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            complement(11041..11853)
                     /gene="hpnC"
                     /locus_tag="WGR74_000670"
     CDS             complement(11041..11853)
                     /gene="hpnC"
                     /locus_tag="WGR74_000670"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ0905143.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYGNFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(11940..12422)
                     /locus_tag="WGR74_000671"
     CDS             complement(11940..12422)
                     /locus_tag="WGR74_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0905144.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHYHH
                     "
     gene            12895..13101
                     /locus_tag="WGR74_000672"
     CDS             12895..13101
                     /locus_tag="WGR74_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905145.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPAAEAPAAEAPATEAPAA
                     EAPAAEAAATEAPAAEAPAAEAAK"
     gene            13211..14578
                     /locus_tag="WGR74_000673"
     CDS             13211..14578
                     /locus_tag="WGR74_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0905146.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYKHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(14759..16783)
                     /locus_tag="WGR74_000674"
     CDS             complement(14759..16783)
                     /locus_tag="WGR74_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEZ0905147.1"
                     /translation="MYYETRSKQKFKKSFIISLFFSILYTSPLLAVDYVYDNSKLTNE
                     QIDRLKKLRDKSSEYWKQETYLLKPTNGSSQNSLKVPALYSKTHQFENINNSKKISFY
                     DKEYTEDYLVGFARGFGVEKRNGEEEKTLQQYFKDCVNTGNSNNDQCEQPSFGNSGPR
                     LIKSDIFALAPQIKNSHINSEILSVGNYIEWLRPTLNKLAGWEEHLYSGLDPFHYITV
                     TDNSHVIGQTISLDQFILENSLWEPRWDSDVGELKTTNADIRFNTKSESLLVKEDYAG
                     GARFRFAYDPKEAKNTALIFEKNVTGTSNIIFENPIDDLKSLDGHQIIKVNGTADKRA
                     FRLSGKHQKGIYTLSLQQRPEGFFTKVQERDDIAIYAQQAQAANTLFNLRLNDKNSDI
                     FDRTLPRKGLWLRLISGHLSQDVQGKTAPVEGNRKGIQLGGDVFSLQNHNYQLSVGLM
                     GGQAEQRSTFRNPDTDNLTTGNVKGFGAGVYATWHQLQDKQTGAYADSWVQYQRFRHR
                     INTEDATERFTSKGITASIEAGYNALLAEHFTKKGNRVRFYLQPQAQLTYLGVNGKFS
                     DSENAHVNLLGSRQLQTRVGVQAKAQFAFTNGVTFQPFVAVNSIYQQKPFGVEMDGER
                     RVINNKTAIESQLGVAVKIKSHLTLQATFNRQTGKHHQAKQGALNLQWTF"
     gene            complement(17149..17595)
                     /gene="smpB"
                     /locus_tag="WGR74_000675"
     CDS             complement(17149..17595)
                     /gene="smpB"
                     /locus_tag="WGR74_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ0905148.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(17650..18660)
                     /gene="waaF"
                     /locus_tag="WGR74_000676"
     CDS             complement(17650..18660)
                     /gene="waaF"
                     /locus_tag="WGR74_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ0905149.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLDIGKPVLAFCPGAEFGPAKRWPTRYFAELGKHYSAAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(18791..18866)
                     /locus_tag="WGR74_000677"
     tRNA            complement(18791..18866)
                     /locus_tag="WGR74_000677"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18831..18833),aa:Asn,seq:gtt)
     gene            complement(19057..19809)
                     /locus_tag="WGR74_000678"
     CDS             complement(19057..19809)
                     /locus_tag="WGR74_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ0905150.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEVRYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            20120..20887
                     /locus_tag="WGR74_000679"
     CDS             20120..20887
                     /locus_tag="WGR74_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEZ0905151.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGGNASPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHRVVADIPADVHRRN
                     ATSLQLSRCANIADRTGLDALGGRRHSRRCPPPQRGLAAIKPLRQHRRRPVRSHIGGN
                     QFADLPHQVDAFVHGKTDAVMLQPRQHAAKRGLRRIVRHFFIRQHRRRIIKRRAHPPG
                     VRQLHRIAHCLRRPVLCGEPFYGFLFKSNPLRLIEAV"
     gene            complement(20836..21115)
                     /locus_tag="WGR74_000680"
                     /pseudo
     CDS             complement(20836..21115)
                     /locus_tag="WGR74_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(21441..21602)
                     /locus_tag="WGR74_000681"
     CDS             complement(21441..21602)
                     /locus_tag="WGR74_000681"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905152.1"
                     /translation="MPSEKKMGCRIWMSDHLIRPNVGLRYSGLNSNQYGVASPCRTIC
                     TVCGFVALS"
     gene            complement(21592..22737)
                     /gene="dapE"
                     /locus_tag="WGR74_000682"
     CDS             complement(21592..22737)
                     /gene="dapE"
                     /locus_tag="WGR74_000682"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ0905153.1"
                     /translation="MTETQSLELAKELISRPSVTPDDQNCQQLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKVPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVGKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARAAIAEICGVETELSTTGGTSDGRFIKA
                     IAKELIELGSSNATIHQINENVRLDDIPKLSAVYEGILARLLAGNAV"
     gene            complement(22851..23471)
                     /locus_tag="WGR74_000683"
     CDS             complement(22851..23471)
                     /locus_tag="WGR74_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEZ0905154.1"
                     /translation="MLTPKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPDYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     KLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(23505..24014)
                     /locus_tag="WGR74_000684"
     CDS             complement(23505..24014)
                     /locus_tag="WGR74_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0905155.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIARYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHVSLYGN
                     WDAVAAEFAKLEADNAYVPDEEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(24108..24659)
                     /gene="azu"
                     /locus_tag="WGR74_000685"
     CDS             complement(24108..24659)
                     /gene="azu"
                     /locus_tag="WGR74_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ0905156.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKTSMGH
                     NIVIGKTEDMDGIFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     DYKFACTFPGHGALMNGKITLVD"
     gene            complement(24940..25362)
                     /locus_tag="WGR74_000686"
     CDS             complement(24940..25362)
                     /locus_tag="WGR74_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ0905157.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLAPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            25493..28243
                     /gene="secA"
                     /locus_tag="WGR74_000687"
     CDS             25493..28243
                     /gene="secA"
                     /locus_tag="WGR74_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ0905158.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTNQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPIQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADEALSDEEKQAQISALEDGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMKEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            28258..28410
                     /locus_tag="WGR74_000688"
     CDS             28258..28410
                     /locus_tag="WGR74_000688"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905159.1"
                     /translation="MPSEHPAPVSDGILPEPPHPTAIPKNPDFVPSVPKTDIPSAPHH
                     DSIRLH"
     gene            28388..30160
                     /locus_tag="WGR74_000689"
     CDS             28388..30160
                     /locus_tag="WGR74_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ0905160.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMVVPKVRGQND
                     NPEVRAERKKKKQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMLTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAESIKNHAAVPETAQV
                     LEYMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIEALKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            30347..32275
                     /gene="rpoD"
                     /locus_tag="WGR74_000690"
     CDS             30347..32275
                     /gene="rpoD"
                     /locus_tag="WGR74_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ0905161.1"
                     /translation="MSRNQNHEEYQDDTRPLSIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            32422..33188
                     /locus_tag="WGR74_000691"
                     /pseudo
     CDS             32422..33188
                     /locus_tag="WGR74_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         8455 a         9406 c         7784 g         7543 t
ORIGIN      
        1 caacgccgta ccggtttttg ttaatccacg ataaatttgc cacaaaaaag ctgcctcaaa
       61 tgaatacccg ggcagctttt tgttgatatg actccaatca gcggtgttgc ggattgtaac
      121 gtttttccaa acgcaggaat atccagccta agaaagtcgt catcaacaga taaatcaggg
      181 cgacggtgta aagcggttct tcataaaccg aataccggcc cgtaatcgta ttctgaacat
      241 acgccaactc cgccacagca atgaccgaca gcagcgagct gtctttcaag agcgtgatga
      301 actcgctcgc caaaggcggc agcatgcggc gcaatgcctg cggcagaatc acatagcgca
      361 tcgcctgcgg ataggtcagc cccaaagaac gcgccgcctc catctgtcct ttgtctatag
      421 actggatgcc cgcgcggaaa atctcacaga tatacgcccc cgagttggcg atcagtgcca
      481 aagaaccggc aatcagcggc ccgtatccgc gacgcagcgc gattgccgcc tcgccgctga
      541 ccaaaatgcc gtctgaagga tggacgaaaa acggaaacca cacatacgcc caaatcacaa
      601 tctgcacaaa cagcggcgta ccccggaaca gcgtaacata cagcagcgaa actttacgca
      661 acgcccacgc cagcacgcgc atcggcgcac cggctttttc caagtgaatc aggcgcgcca
      721 acgccaacaa cagacccaat accgaaccgc ccgccgtcgc cacgaccgtc agccccaagg
      781 tcgtcagtgc gccgtaaagg aacatccagc ggtattcgta aataatgtca aaacgaaaat
      841 ccatataccg tccgtatcaa aaaccggcgg aactgccgcc gttgcaaaat aatccgccat
      901 tttaccgtaa aaaccgccgc ctgaactttt ttatcgcggc agacggcggt tgcgcgtctc
      961 cgcaaaaatg cagggcgcgc ggttttcaga cggcatttgc cgttcaaagc cgtgcgctgt
     1021 ctttaccaaa tgcccaacca ttcgcccacg gcatccatcc aatccttatt gcccccgccg
     1081 ctcctgcctg cccggcggta cgcccacggc gcttgcggat ttttagcttt ccacaatcct
     1141 ttgcgttccc tttccgcctg aatttgagcg tcggcataat cggcaaaatc cgccttatcc
     1201 tgctgttctt tagcataact tttataatgc cacgccgccc cgtcctgcac ctgcatcagg
     1261 ttcaaatcgg ttttgccgac agaaacctgc gccacttcgc gctggtagcg gtcggtgtcg
     1321 aacacgcgca cgctgacttt cctgccttcc gccgccgcac gcaggttgtc gcgcgaacgc
     1381 gtgccgtaag cctgtttcat ctccggcgcg tcgatatacg ccatccggat tttgtgtttc
     1441 gcgccgtcgc cgtcgataac gtgaagggtg tcgccgtcat agactttgga caccgtgcct
     1501 gtgtagcggt ggccggattt cgccgatgct cggcggcggg cgggcgcgtc ggaacccgcg
     1561 tcccctgccg cgccgagtac gtcgagtacg gcaaccgccg tccgcaccgc ctcgctgccg
     1621 taccccgtat aacccaacgc acccaaaagc gacaaggcga cgggaagcca tttcatgatt
     1681 tttttaatct gcatattttt caaatgccga tgccgtctga acatatcgga atcggatttc
     1741 agacggcatc ttaacgtcag gattaccctt ggcagggata gatgactttc gcaccctctt
     1801 ccgtccccaa aatcaacaca tcggcggcat cgcgggcgaa tatgccgttt tcgagcacgc
     1861 cggtgatttt gttgatttcg tcttccatcg tcagcggctg atcgatattc aagccgtgga
     1921 catcgacgat ttggttgccg taaaacgtgg tgtagccgat acgcagttcg ggctgtccgc
     1981 ccatagcgag cagtttgcgc gaaacaagag agcgcgcgct ttcgacgact tccacaggca
     2041 gagggaattt gcccaaacgt gaaacatatt tgctttcatc cgcaatgcag acgaattttt
     2101 cggatgcgct ggcgacgatt ttttcgttga ggtgtgcgcc gccgccgcct ttaatcattt
     2161 gcagggcgtg gttcacttca tccgcaccgt caatatagac cgccaacccc gatacttcgt
     2221 tcagggaaac gacgggaata tcatattggg caagcagttc actggatttt ttggaagtag
     2281 ataccgcgcc tttgattttt ttgccgctct tacccaaggc ttcgatgaaa aagttgatgg
     2341 tcgaaccggt accgatgccg atgtattcgt tttcgggtac gaattcgact gctttttcgg
     2401 cggcgatgcg cttgagttcg tcttgtgtcg tcatattttt gtcctttggg aaaccgtatc
     2461 aacaaacagc cgccatctta acattttttt gcacgtcctg cccgccgcgt tcaaatcctt
     2521 accagcaata ccgccgcctg cgcctctatg ccttccatgc gcccgagata accgagtttt
     2581 tcgttggttt tgcctttgat gttgacgcac gaaatgtcta tgcccaaatc ggcggcgatg
     2641 ttggcacgca tttgcggaat atgcggcgcg agtttgggtt tctgtgcaat cacggtcgta
     2701 tcgacattga ccgcctgcca accctgcgcc tgaacgcttt gatacgccgc acgcaaaagg
     2761 acgcggctgt ccgcatcttt gaactctgcg gcggtgtcgg ggaaatggct gccgatatcg
     2821 cccaaacctg ccgcaccgag cagcgcgtcg gtaacagcgt gcagcagcgc atcggcatcg
     2881 gagtgtccga gcagcccttt ttcaaatggg atttcaactc cgccaagtat cagctttctg
     2941 ccttcggtca gttggtggac atcgtagccc tgtccgatac ggatgttcgt catcgtttgt
     3001 gttcctgatg ttttgaattg aagttcagac ggcattgagc agcagcctga cgatgtatgc
     3061 gtcctgcggc tgcgtcagtt tcaaattgcg cgcgtcgccc tgcaccagca aagggcggat
     3121 gcccaatttt tccacggcgg acgcttcatc ggtaatgccg tccaagtttt ccgcagccaa
     3181 tgcgcggtgc agcagccccg cgcggaaaag ctgcggcgtt tgtgcctgcc aaaggctcgt
     3241 ccgctcgaca gtggcactga tttgtccgcc ttctgcgcgt ttgagcgtat cggcaatgga
     3301 aattgccaaa atcccgcctt cggcggcgtt gcccgcctgt tctatcaacc gcgtcaaagc
     3361 ttcagacggc aggcagcagc gcgcggcatc gtgtaccaga atattgtcat ccgccgatgc
     3421 caagccgatt tccaacagtt ttgccacacc gtttcggacg gtttcggcgc gggtctgtcc
     3481 gccgtttttc cacacccgaa cctgtggaaa tgccgtctga accttatcgg caaacgtgtc
     3541 ttcgggcgag acgacaacga cggtcaaatc gacggcctca tgccgttcaa aaatcccaat
     3601 cgtatgttct aaaacggttt tgcttccgat ttcgacatat tgcttgggtt tgtccgcacc
     3661 gaaacgcgcc ccgatgccgg cggcgggaat cagcgcgata tttttgcgct tcatgcgtcc
     3721 gtcccgccgt tttcagacgg cacggcttcc ttgcgccaga tacaggcttc gcccaagccg
     3781 tccaaatatt gcccgtgcgc cgccaactcg ttttcgtccg ccctgatgac tttcagtttg
     3841 ccgctgcgtt tggtttcggt atgcaccacg ggtttggttt ccattttttc ctctgcggcc
     3901 gcacccatca ggtcgaactg ccgccgcgtc atagcaagat agacttcgcc caaaagttcg
     3961 cagtcgatca atgcgccgtg caggacgcgc ttgctgcggt cgacggaaaa acggttgcac
     4021 aaggcatcca ggctggcttt ctgcccgggg aacatttcgc gcgccatcgc cagggtatcg
     4081 gtaacggtac agccgagttc ctcaacggtc ggcaacccca tccggcggaa ctccatattg
     4141 aggaagccca cgtcgaattt ggcattgtgg ataatcagtt ccgcaccgcg caggaaatcg
     4201 gcaatctgcc tgccgacctc tgcaaacggc ggcgcgtttt tcccttccaa aacctgtatc
     4261 gtcaagccgt ggacgcgtgc cgcctcttcg ggcatatcgc gctcggggtg gacatagagg
     4321 tgcaggtttt tgtcggtcat ttggcggttg accatttcca aaccggcaaa ctcgaccaag
     4381 cggtcgccgc cgtcggcata cagaccggtg gtttcggtat cgaggatgat ttggcgtgtc
     4441 gtcatatcgg tgtctttctt ctatcttcgt aaattgctta ttttttaagc aatgtatttt
     4501 tctgttttca tttcaatgca caaacccact tattcacagt gtgttcacaa cattgggcag
     4561 gcggattgtg tattttgggg acaatttttt cagacggcat tcaaggtttt ttcctgattg
     4621 ccgccgcgcc taaaaaccgc ctttcgcgct taatcaaaaa taccgacaac ggaatattgc
     4681 ccaaagcgac aatcagatac aacaaggaaa tgctgtcaaa caaaaacagc aacaccgcgc
     4741 tcaaaacggc agcggaaacc ataaaaatac cgttaacgat attgttggcg gcaacggcgc
     4801 gggcgcggaa agtctcgcta ctggcggttt gcagccaggt atagagcgga acggagaaaa
     4861 atccgccgaa aaagccgatc agcgtcatca ccgccatcac gggatatgcc catccttgcg
     4921 ataaaaacca aaaaatgccg ttcagccctt caaaacggtg tccgtgcgtc agccacacca
     4981 aaaccaggcc gcaaaccgtc aaacccaacg caccaaccgt tacccaagcc aacatcaggc
     5041 gttccctgct gaacttggca cacagtaccg aaccggcggc aataccgatg gaaaacagag
     5101 caagcatcag gttgaaaaca ttgtcgttgc cgcccagatg gatttgggta aaggtcggca
     5161 gttgcgtggt ataaaccgcg ccgacaaacc aaaaccacga aataccgata atggcggtaa
     5221 aaacgggctt gtgccgcacc gtttcacgca gcagggattt tgtgccacgg acaatattcc
     5281 actcaatttg tgtatcggca gccttggcgg gtacggacgg cataaacagg ctgccgaccg
     5341 tgcctccgac ggcgaccagc aaaaccagta tcccgacaat ataaggcggt acacctgcca
     5401 ccgccgttcc caaaatctga ccgaacagga tggcgacaaa cgtacccgat tcaatcaggc
     5461 tgttgcccat catcaactct ttgtcgtcga gataatcggg caggatggcg tatttcagcg
     5521 gcccgaacag cgtcgattgc gcgcccatgc aaaacagaca cgccaaaagc agcggggcag
     5581 accggatata aaacccgtat gccgccaccg ccataatgat catttccagc accttgaccc
     5641 aacgcgccaa aacggccttg tcgaatttgt tacccaactg ccccgacagc gaggaaaaca
     5701 ggaaatacgg caaaataaac agcaacgcgc ccaagttcaa catctgtccg gcaggcagga
     5761 agccgttttg ccccaaaccg taaaacccaa tcatcacaaa cagcgcggtt ttgaacacat
     5821 tgtcgttgaa cgcgccgaga aactgcgtag cgaaaagagg tgcgaaacgg cggcttttaa
     5881 ccagtcccaa accgcctttt ttagcgtaca tcgttttccc tctctttttc aatcagttta
     5941 cttgtcgaat catcatccat caggatgcgg tgcgccggcc cttccaagtc gtcaaactgc
     6001 ccgtttttgc ccgaccacca aaaaaaccag ccgatgacaa acgccaaaat aatgctgatg
     6061 ggcaccaata taaacatgct ttccatcaca tattccctgt caaatcgttc aaaacaaaag
     6121 tctgccccga cacggtcaga tattcgttac gcaaagttcc gacgggagct tcgtcaaaaa
     6181 acagctcgat acggtctttg accacgcgcc aatattgggg gatttccgtc tgaccgaacg
     6241 gcgacaggac atgattttcc attccgcctt caagtttgac ggcaaaacgc ccgctttgcg
     6301 gccgtgcttc cgattcgtcg tcggcaagca ggatgaaaaa gcctatatgc cgtcccgatt
     6361 ggtcatgaat actgaaataa tgcataaatt tcccacccgc cttttttcag acgacaccaa
     6421 ctaaaaacag ggcgaatgta ccagtttgga cgggaagaat gcaaagaaat tctccctccc
     6481 ccagccgaaa acaccggcaa accgcatatc cccctttttt ccgtcaaaat gcccgacttc
     6541 cgccattttc acgcaaacgc ccgattaagc caagcaattg caaagatttt ttgctagaat
     6601 agcctgcttc ttttatcaac cttttcagac ggccccacta ctttcccgcc caggaaggca
     6661 aaacggattc ggcacgaatc cggttagtat ccgtgtccga ttccaatgcc gtctgaaact
     6721 ttccggagta agaaaatgtc ccaaaaattg atcttggttt tgaactgcgg cagctcgtcc
     6781 ctcaaaggcg cggtcctgga taacggcagc ggcgaagtcc tgctcagctg ccttgccgaa
     6841 aaactcaacc tgcccgatgc ctacatcaca ttcaaagtaa acggcgaaaa acacaaagtc
     6901 gatctgtccg cacatcccga ccacaccggc gcggtcgaag ccctgatgga agaactcaaa
     6961 gcccacggcc tcgacagccg catcggcgcc atcggccacc gcgtcgtcag cggcggcgaa
     7021 ctgtacagcg aatccatcct cgttgacgac gaagtcattg ccggcatcga aaaatgcatc
     7081 ccgctcgccc ccctgcacaa ccccgcccac ctcttgggcc tgcgtgccgc gcaaagcatt
     7141 ttcaaaggcc tgcccaacgt cgtcgtattc gatacctcct tccaccaaac catgcccgaa
     7201 gtcgcctaca aatacgccgt tccgcaggag ttgtatgaaa aatacggcct gcgccgttac
     7261 ggcgcgcacg gtaccagcta ccgcttcgtc gccgacgaaa ccgcgcgctt cctcggcaaa
     7321 gacaaaaaag acctgcgtat ggtcattgcc cacttgggca acggcgcgtc catcaccgcc
     7381 gtcgccaacg gcgaatcgcg cgacaccagt atgggcctga ccccgctgga agggctggta
     7441 atgggtacgc gcagcggcga catcgatcct tccgtattcg gcttcctcgc cgaaaacgcc
     7501 aatatgacca tcgcccaaat cactgaaatg ctgaataaaa aatccggtct gctcggcatt
     7561 tccggcctgt ccaacgactg ccgcaccatt gaagaagaag ccgccaaggg gcataaaggc
     7621 gcgaaattgg ccttggatat gtttatctac cgccttgcca aatacatcgg cagtatggcg
     7681 gttgccgcag gcggtttgga cgcactggtc tttaccggcg gcatcggcga aaactccgac
     7741 atcatccgcg aacgcgtgat cggctacttg ggcttcctcg gtctgaacat cgaccaagaa
     7801 gccaacctga aggcacgctt tggcaacgcc ggcgtgatta ccactgccgg cagcaaagcc
     7861 gttgccgtgg tcattccgac caacgaggaa ttgatgattg cacacgatac cgcccgcttg
     7921 agcggtctgt aagattcaat ccccacgcga actgcctccg aaaacggagg cagttttttt
     7981 atccggcttt ccatgcttaa acagcaccgc ctcttttcag acgttgacgg ttgcagccgc
     8041 ttacctgaac cttatagtgg attaacaaaa accagtacag cgttgcctcg ccttagctca
     8101 aagagaacga ttctctaagg tgctcaagca ccaagtaaat cggttccgta ctatttgtac
     8161 tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactatatcc gactttccat
     8221 acttaaacag cactgcctct tttcagacat tgacggttgc agccgcccac ctgaacctta
     8281 atgattgact attttttaat catgttatta ttttccataa aatacatgac attaagatgt
     8341 ttttccacaa aagatacaca caccggcaaa caccggctgt gtttatcttt tcttatgcct
     8401 attttttaat catcgtattt ttatctttta atttcaatac gcaaactaac ttatacacac
     8461 ggttttcaca tctttggact gcttccgtgt gtatagcgga tattgccgtt ttcctttctg
     8521 acaaaaatgc cgtctgagaa cttcagacgg catttgaaac atcggaatca gcggttttgt
     8581 tcataccact cgataaactt gtctgctttg acaaaaccca gcagcggctc gctgcggctg
     8641 ccgtcggagc ggacgacaaa cacgcccggc ggcccgaaca gaccgtattc tttcaacaac
     8701 gcctgatgtt cgggcgtgtt ggcggttacg tcgatttgga aaaagcgttc catatcgact
     8761 gcctgatgca cttccggctg attgagcgtg taagccgcca tttctttgca ggaaatgcac
     8821 cagtcggcat aaaaatccaa aacgacgggt ttgtcgggat gttctttcaa cgccgtatcc
     8881 atcgctgcct tcagcgcggc agtatcggca aacattttgc cgtgttccga agatttgcct
     8941 gcttcggccg gcggattgag ggtcaggaaa tggtgcagcg cggtcgtttt gccgtttgcg
     9001 ccctgccagc cgaaccacgc gccgcctatc agcaatatgc tgcccaatgc gaatgccaca
     9061 gctttcggac ggcgtttctg cctgcgtccg ttgaccagca gcatacaggc aggaaccagc
     9121 atcagcagcg tgtacagcgc gacgacgaga taatagggca agtgcggcgt ggcgaggtaa
     9181 acggcgacgg caagcaggat aaagccgaaa gcgtatttga cggcattcat ccaatcgcct
     9241 gccttaggca ggatatgccc gccgaacgtg ccgatggcaa tcagcggaac gccggtgccc
     9301 aacgccaaag tgtaaagtgc caaaccgcct aaaaccgcat cgcccgtctg accgatgtag
     9361 cccaaagcaa atgccagcgg cggggcgacg cacggcccga caatcagcgc ggacaatatg
     9421 cccataataa agacggaaac gattttaccg cctgaaagcc tgctgctttg attctgaaaa
     9481 tacgactgca cggcgttggg aagctggatg ttgaacagcc cgaacataga cagtgccaag
     9541 acgaccatca aagccgatgc cgccaacacg acccaagcct gctgcaacca tacggtcagc
     9601 agtgcgcccg tcagtccggc aacaatgccg accagcgtat aagtcagagc caaaccctga
     9661 acataaacga cggacagcac aaacgcccgc gccttgcccg cctttttgtc gccgaccaca
     9721 atactggaaa caatcggcaa caggggatac atacaggcgg taaaactcag acccaaaccg
     9781 gcgagaaaaa acgccaaaag attggcgttg agcgtatccc aagacagctt gaaacggctg
     9841 tcgccgccct catccccctt cgggggcggc agcgccccgc tgccgttttg agaggaaggc
     9901 tgcaaaaagc ggtctttggc ggatgccggt tcgtcggttt gcggatggta agtgccgttg
     9961 ccggaaatat caaactcggt atccacgggc ggatagcaca cgccggcttc ggcacagccc
    10021 tgataggtca aaaccaattt atacggttcg ccgacagcct ttgcataagg aaaggcaacc
    10081 tgcgcttcgt gatggtaaac cgtctgcctg ccgaaaaact cgtcttcctt ctcttcgccc
    10141 ttgctgaaag aaggctgtcc caacaaatcc gccggatcgg tcttgccgac gattttcgcc
    10201 tgatacatat agtatccgtc ggcaatcttg aaacggacgt tcacaccgtc gtcggcaacg
    10261 gcaagctccg gcacgaatgc cttttccggc ggcagcagat cgttcgcatc cagcgcgaaa
    10321 gctcgtccgc acaacatcaa aaatacggcg aacaggcaaa tcagtttttt cataatcgaa
    10381 tccgtttcag acaaataatt tgtctgcatt ataaatggta aggttgacgg tgggatttaa
    10441 tttatgtaaa acccgccatt atccgaacct atttccataa ccatcttatc gaacccgcca
    10501 tgtacgatgt caatacccac gatgtccgcc gctttttcgc ccgcgtgtgg cagcagcggc
    10561 tcaatccgct gcaactgagc gcactggaac agaaagccct ccgcattgtc gaagcccatc
    10621 ccgaatacca ccgctatctc gaacgcatcg aagaccatct ggacaccgac tggctgcccg
    10681 aaaacggcga aagcaacccc ttcctgcata tgtcgctgca tctgtccgtc caagaacagg
    10741 cgggcataga ccagccgcac ggcatacgcg caatccacga caccctgtgc gccaaacgcg
    10801 gctggctgga agccgaacac gaaatgatgg aggcactggc ggaaacactg tggacggcgc
    10861 aacgctacgg caccggtttg gatgtcaatt tctacatgac ccgactgcgc aaactcatcg
    10921 gcttgggtgc agaagatcaa gccaggttga acccacatga aatcgcctga ccataccaac
    10981 cgcctgcaaa atgccgtctg aagcggaaca acccctttca gacggcatgc atttccccca
    11041 tcatttccac aacgcgcgtt tcagcataat caaccaatcc ttcttatcca aaacggggcg
    11101 ttgtgcaaac acatcgtatc ggcacgcgtc cagtttctgc aaaatcaact gcgcccccaa
    11161 cacaatcata cggagttcca aaccgatacg ccctttcaat tcgcgcgcta aaggcgaacc
    11221 cgccttcagc atacgaaatg cgcgccggca ctcatacgcc atcagccgct gaaacgccgc
    11281 atccgcccta ccctccgcaa tctgtccttc ggaaacaccg aatttcaaca aatcgtcctg
    11341 cggaatataa accctgccct tttgccaatc cacagccaca tcctgccaaa aattcaccag
    11401 ttgcaaagcc gtacagatgc cgtcgctttg cgccacgcac accgcatccg ttttcccgta
    11461 caaagccagc ataatgcgtc cgacaggatt ggcagaacgt cggcaataat cggccagctc
    11521 gccgaaattt ccataccttg ttttaaccac atcctgagaa aatgcagaaa gcaaatcata
    11581 aaacggctgc aaatccaaac cgaacggcac aaccgcctcg gcatccaatc gtgcaatcaa
    11641 aggatgcgcc gaccggccgc ccgatgccaa cacgtccaac tcgcgccgca aaccctccaa
    11701 ccccgccaac ctggcttcag acggcatact gccctcgtcc gccatatcgt ccgccgtccg
    11761 tgcaaacgca tacaccgcgt gaaccggctt cctcaaacgg cgcggcaaaa tcagcgaacc
    11821 gacgggaaaa ttctcataat gcccaaccga cataccttct ccatccatca aacaaaatgc
    11881 cgtctgaaac ggaacaaacc cttttcagac ggcatcagat acctccaagc tgccggcaat
    11941 cagtggtggt aatgaccgtg cgggccgtgg acatgaccgt gtgcgatttc ctcatcggat
    12001 gcatcgcgca cgctttcaac cgtagcctta aagcggattt tcatgcctgc caaaggatgg
    12061 ttgccgtcca ccaccgcctt gccgtcggca acatcggtta cacgatagac gacaacatcg
    12121 ccggtttcag gatcgtcggc ttcaaacatc atgccgactt cgacttcaac agggaacacg
    12181 cccgcatctt cgatacggac caactccgga tcctgctcgc cgaacgcatc gtcgggcgac
    12241 agcgccacat cgaccgtatc gccggcatcc ttaccgtgca acgcctcttc caccaaaggg
    12301 aaaatgccgt cgtaaccgcc gtgcagatac gcaatcggtt cttcggtttt gtccaaaagc
    12361 tgattgttgg catcatacat ctcataatgc agcgaaacca cggaattttt cacgatagcc
    12421 atatttgtcc tttcaggaac agcagattga ttacaggcgc attctaacac aaccgccgcg
    12481 ccggccgatt accgttaacc tgttcataaa ctgtacagca catatttcaa tgtaaatctt
    12541 tgttatttta ttgcggtgta acttttttac aacattctta aaaccattcc gacctgtctg
    12601 ccgactttcc caatccgcct taataaatca tacaagatac tgaaattata ttaatctcta
    12661 taatatttat ccctatcgaa tttttaacag caaaaccgtt ttacaggatt tatcaatccg
    12721 cccgccagaa aacttttcat tcaaaccttt ttcccatctg tacgacattg caatccctta
    12781 ttccatagtg cataattacg caaattcagc gatgaatttc caacctggtt tgtagtatgg
    12841 tcgataaaga cctatttgtt tcaataattt aaattggttc taaaggttac taaaatgaaa
    12901 aaatccctgt ttgccgctgc tttgttgtct ttggttctgg cagcctgcgg cggtgaaaaa
    12961 gccgctgaag ctcccgctgc tgaagcacct gccgccgaag ctcctgctac tgaagcacct
    13021 gccgccgaag ctcccgctgc tgaagctgcc gctaccgaag cacctgccgc tgaagctcct
    13081 gctgccgaag ctgcaaaata agcattttcc gcttgcaaaa aagcaggata cgttcagtat
    13141 cctgcttttt tgatttttca gacggcatca gattcccttc ctcaatcttc tccctaccct
    13201 tccgacaaac atgcctgacc ttcataccga attttcccga ctcctgccgg cagatgaaat
    13261 tgccgaactt tctccgacgc ttttaaaaga ccagcgcaac cgctttacgt ctgcaccaga
    13321 catcattttg cagccgcgca gcgttgaaag cgtgcaaacc attatgcgtt tttgttacaa
    13381 acaccgtatt ccggttacgc cgcaaggcgg caataccggt ttgtgcggcg cggcagtatc
    13441 ggaaaacggc gtattgctga acctttccaa actcaaccgc atccgcagca tcaatttgtc
    13501 agacaactgc ataaccgtcg aagcaggttc cgtactccaa accgtccaac aggcagccga
    13561 agcctcaaac aggctgttcc cactcagtct cgccagcgaa ggctcgtgcc aaatcggcgg
    13621 caacatcgcc tgcaatgccg gaggtttgaa cgtattgcgt tacggcacga tgcgcgacct
    13681 ggttatcggt ttggaagtcg tcctccccaa cggcgaactg gtttcccatc tccatcccct
    13741 gcataaaaac accaccggct acgacctgcg ccatctgttt atcggcagcg aaggcacact
    13801 gggcatcatc actgccgcca cactcaagct ttttgcccgt ccctcggaca aggcaacggc
    13861 atgggtcggc atacccgaca tcgaatccgc cgtccgcctg ctgaccgaaa cccaagctca
    13921 ctttgccgaa cgcctatgca gttttgagct gatcggccgt tttgccgccg agttgtcttc
    13981 cgaattcagc aaattccccc tgccgacaca ttcagaatgg catattttac ttgagttgac
    14041 cgactcatta cccgacagca atcttgatga tcggcttgtc gaatttcttt ataaaaaagg
    14101 atttaccgac agcgtattgg cacaaagcga acaagaacgt atccatatgt gggcattgcg
    14161 cgaaaacatc tccgcctccc aacgcaaact gggcaccagc atcaaacacg atattgccat
    14221 tcctatcgga cgcgttgccg actttgtccg ccggtgcgcc aaagatttgg aacagaattt
    14281 taaaggcata caaatcgtct gcttcggaca tcttggcgac ggcagcctgc actacaatac
    14341 tttcctgccc gaaatcctca gcaatgaagt ctatcgttac gaaaacgaca tcaacgacgc
    14401 agtttatcgc aacgttcttg cctgcaacgg cacgattgcc gccgaacacg gcataggtat
    14461 catcaaaaaa cagtggctgg acaaagtacg cacgcctgcc gaaatcgccc tgatgaaaag
    14521 catcaaacaa caccttgatc catataacat tatgaatccg ggcaaactgc ttccgtaacc
    14581 ggcatttctg atttgcatac acaacaaaga aagggacaat agatccgatt gtcggtttag
    14641 cgcgagctcg tgagtgcggt aaaaaattgg tggaaatgac acgaaaaatg accgcatttt
    14701 taaaataaaa aaatcggcag tgaatttctc tgccgatttt attttgttac aacttaactt
    14761 aaaacgtcca ctgtaaattc aatgcgcctt gtttagcttg atgatgtttg cctgtttggc
    14821 gattgaatgt ggcttgtaaa gttaagtgag atttgatttt cactgctaca cctaattggc
    14881 tttcaattgc cgtcttattg tttatcactc gacgttctcc gtccatttct accccgaaag
    14941 gtttttgttg gtagattgaa ttgacagcaa caaatggttg gaaagtgacg ccattagtga
    15001 aagcaaattg cgctttagct tgaacgccaa cccgagtttg taattggcgg gagccaagta
    15061 aattcacgtg ggcattttcg ctatcgctga attttccgtt tacccccaaa taagtcaatt
    15121 gcgcctgtgg ttgtaggtaa aaacgaacac ggttgccctt tttagtgaag tgttccgcca
    15181 ataaagcatt gtaacccgct tcaattgagg cggtaatacc ttttgaagta aaacgttctg
    15241 tagcatcttc agtgttaatg cggtggcgga agcgttgata ttgtacccag ctatccgcat
    15301 acgcacctgt ctgtttgtcc tgaagctgat gccaagtggc gtaaacgcct gcaccaaagc
    15361 ctttcacatt tcccgttgta agattgtctg tatctgggtt gcggaaagta ctgcgttgtt
    15421 ctgcttgtcc gcccattaag ccaacggaaa gttgatagtt gtgattttgc aatgagaaaa
    15481 catcgccacc aagttgtatg cctttacgat ttccttctac tggtgcggtt ttaccttgta
    15541 catcttggct taagtgacca ctaattaaac gcaaccacaa gcctttgcgt ggtaaagtgc
    15601 ggtcaaaaat atcgctgttt ttgtcgttca aacgtaggtt gaataaagta ttggcagctt
    15661 gagcctgttg tgcataaatc gcaatatcat cgcgttcttg cactttggta aaaaagccct
    15721 ctgggcgttg ttgtaaagag agtgtataaa ttcccttttg gtgtttgcca gaaagacgga
    15781 atgcgcgttt atctgctgtg ccatttactt tgataatttg atgcccgtcg aggcttttta
    15841 aatcgtctat tggattttcg aagatgatgt tggaagtgcc agtaacattt ttctcaaaaa
    15901 ttaatgcagt atttttcgct tctttaggat cgtaagcaaa acgaaaacga gctccgccag
    15961 cataatcttc tttgacgagt aaactttcac ttttagtatt aaaacggatg tctgcattcg
    16021 ttgtttttaa ttccccaaca tcagaatccc aacggggttc ccagagagaa ttttctaata
    16081 tgaattgatc caaactaatt gtttgtccga taacgtgtga attgtctgta accgtaatat
    16141 agtggaatgg atctaaacca gaatatagat gctcttccca accagcaagc ttattaagtg
    16201 ttgggcgaag ccattcgata taattaccta cagataaaat ttcgctgttg atatggctat
    16261 tttttatttg tggagctaag gcgaaaatat cgctctttat taatcttggg ccagagtttc
    16321 cgaaggatgg ttgttcacac tgatcattat tggaattccc agtatttaca cagtccttaa
    16381 aatattgctg gagtgttttt tcttcttccc catttctttt ttcaaccccg aaacctcggg
    16441 cgaagccaac aaggtaatct tcagtgtatt ccttgtcata aaaagaaatc ttttttgagt
    16501 tgttgatgtt ttcgaattgg tgtgttttag agtatagtgc gggaactttg agagagttct
    16561 gagagctacc attcgtcggt tttagaagat aagtttcttg tttccagtat tcgctgcttt
    16621 tatctctaag tttttttaag cgatcaattt gctcatttgt taatttagaa ttgtcgtaaa
    16681 cgtaatcaac agccaaaagc ggagaggtat aaagaataga aaaaaataga cttataataa
    16741 atgatttttt aaacttctgc ttgcttcgag tttcataata aattttcctt tgtcaagtaa
    16801 aaataaatgg ggcgtggatt ttagcataga attgaacaaa aaatgtcatt tagctcacat
    16861 ttttctctat ttatttctta tttattaaaa gtaaacgttt gctttttgct attttgtcaa
    16921 gccagtttga aaatgtgtat aattgccctc gttatttaca aaaatttcag gaaaaatgac
    16981 cgcactttac ccttggctaa tgccaattta tcatcaaatt gctcaaacct ttgacgaagg
    17041 tttggggcat catgccgtac tgattaaagc ggatgctggt ttaggtgtag aacgtttaca
    17101 catcaggcgg cagccttgcc cataccgtct gaagcactgt ttccacaatc agcgcgtatg
    17161 cttaatcaac cgctgtttct cgcgtttcca atccgcctct ttcatactct gacgtttgtc
    17221 gtgctgtttc ttaccttttg ccaaaccgat ttccatcttg atttttccgc gcgaaaaatg
    17281 caaatccagc ggcacgatgg tgtagccggc acgttcggtt ttgccgatta atttgttgat
    17341 ttccgactgg ttcaacaaga gcttgcgcgg acgtacggca tctggtttaa tgtgtgtcga
    17401 ggctgtgggc aaagccgtaa tatggcagcc gaccagataa aacgcgtctt ttttccaata
    17461 gatataactc tctttaagct gtacgcgcgc ggcgcggatt gctttgactt cccagccttc
    17521 caagaccaaa ccggcttcaa tccggtcttc aatgaaaaaa tcgtgaaatg cttttttatt
    17581 gttcgcaata gccataaaca tcctatcaat atccgccgtc agacggcata aacccgaaaa
    17641 cagaacccat cataccgcct cttcaaccgc ctgcacaatc ttctcgggat acagcctgtt
    17701 gaggcagtcg gtatgcccca acggacattc acgtttgaaa cagggcgaac attccaagtg
    17761 taggctgacg attttcgccc tgtcgctcaa aggcggcgta tgcgtcgggc tggaagaacc
    17821 gtaaaccgcc accaccttcc tgcccaatgc cgccgccaag tgcatcagcc cgctgtcgtt
    17881 gcacacgacc gtgtccgcca acgacagcaa atccattgcc tgcgacaaat cggttttgcc
    17941 gcacaaattg acacacatac cgtctgaaag gcagttgatt tcctcggcaa tttcatcatc
    18001 tttttgcgaa ccgaacagcc aaacctgcca acccgccgcc gaataatgtt tgcccaactc
    18061 ggcaaaatac cttgtcggcc aacgctttgc cggcccgaat tccgcacccg gacaaaaagc
    18121 cagaacaggc tttccaatat ccaagccaaa ggtttcgaca gaaatttccc gccgccgttc
    18181 atcaatggaa aactcgggga atcccgaatg cccgtcaaaa tcctcctggc tcggatgcgc
    18241 gagagccgta tatctgtcca ccatcaaagg cagacgttcc ttatccagcc tgcgtatatc
    18301 gttcaacaga aaataacggc tttcaccgac ataacccgtc cttttaccga tacctgtcgc
    18361 cagcgcgatg attgccgatt tcaaagaacc gggcaacacg ataacctgat cgtatccgcg
    18421 ccgccccaaa tccctaccga cccgccaacg gcgtttcaac tccaacgcac cgtgtccgaa
    18481 cggattttca agaatctcat tcacttccgg catacgctca aacaccgcca tcgaccactt
    18541 cggtgcgaac acatcaatcg tgcaaccggg gtgaagttcc ttcaaacggc ggaacaaggg
    18601 ctgggtcatc acgcagtcgc ctatccaact gggggaaata atcaggattt tgatggacat
    18661 aacaagaaac cgaaatcaga caggcggaat tttaccgcga aaccgttgga aaacctatct
    18721 tgtctcattc cgaacgccgt acgtgcaaat atgaaaaagc ccgaacattc aagttcgggc
    18781 ttcaaaattc tggctccccg acctgggctc gaaccaggga cctgcggatt aacagtccgt
    18841 cgctctaccg actgagctat cggggaatgg ggcgtattat agcgtccgga aaaaatgtgt
    18901 caatccttaa ttttggaaaa atgggcgaca aaacgacaag catatgaatc agaaagacat
    18961 taagaccgat gccttaaaag gattgccgtt gtatgaattt ccacagccgt catcacacca
    19021 tatttaagcc cgatgagccg ttctgccctc ccccgcttaa aacaatgccg tctgaacttc
    19081 gccgcgttcc aaagccagta aaaactgttt gcggttcaac ccgcccgcgt agccggtcag
    19141 tttgccgtcg ctgccgatga cgcggtggca gggaatcagg atagatactt tgttctgccc
    19201 gttggcggcg gcaacggcgc ggacggcttt ggggttgccc aaacgctgcg cctgctcctt
    19261 gtagctgcgc gtttcgccgt aaggaatcgc caagagcgcg tcccatgcct gcttttgaaa
    19321 ctcggtgcca atctgctcca aaggcgtggc aaaggttttc agacgaccct tgaagtataa
    19381 gtccaattcc tgccgcaaaa gttgcgtccg ctcatcctcc cgaaacacaa accgtccgcg
    19441 caaggctttt tggacggcgg caatttcctg ttccaaatgc ttctgtccga caaactccag
    19501 cagacacaat cccttatttc cgaacaccgc cagcatctcg cccaaaggcg tggcaatggc
    19561 ggcacacacc agctcgttca agctgtcggg ataacgcact tccaacagac ggatggctcg
    19621 gcggatacgg acatattctt caggcgcgca gccgatattg tcccaaaaat cccgatcgaa
    19681 ctgcccggct tcgcattccg tcagattggg atgcggcata acgccgcact caaaaacccg
    19741 ggtttcgagg caatggcgga tttcatccca ttttgacggc agattgttta aggaaggcag
    19801 ggtaatcatt cgtttgctcc gtatctctat catggatttg gcggcaaaat ccccaattct
    19861 cgtcattccc gcacgccgat gcaggaacgg gctatgacgt aaatcttgag ggtcgggttg
    19921 cggcaatgcc taaatatgcg atatttccaa agcctcaggg aaaggaatta aagacttcag
    19981 acgacctgca aaggctcgtc tgaaaccacg atttttgcat ttgcgcattc tggcacatca
    20041 tccaaccgtt tcggcacatt cctgccgccg ttgacagcct ataatgaatc cacttattca
    20101 tcaagcaaag gaatcatcta tgcaaacccg catcctctcc gccgtactgc tggctttttc
    20161 aaccgctgcc tttgccgggg gcgcattcac gctgcaattc gacaacccgt ccgaagacgg
    20221 cggcttcacg caaaaccagc ttttgagcgc gccttacgga tttggctgtt cgggcggcaa
    20281 tgcttcgccc gcgctgtcgt ggaaaaatcc gcccgccggg acaaaaagtt tcgtcctgac
    20341 cgtttacgat aaagacgcgc cgaccggact gggctggatg caccgggtgg tcgccgacat
    20401 tcccgccgat gtccaccgcc gcaacgcgac ctcgctgcaa ttaagccgct gcgccaacat
    20461 cgccgaccgg actgggctgg atgcactggg tggtcgccga cattcccgcc gatgtccgcc
    20521 gccgcaacgc ggcctcgctg caattaagcc gctgcgccaa catcgccgac gaccagtccg
    20581 cagccatatc ggcggtaatc agtttgcgga tttgccgcat caggttgacg ccttcgtaca
    20641 cggcaaaacc gatgccgtca tgctgcaacc acgccaacac gccgcaaagc gcggcctccg
    20701 ccgcattgtg cggcacttct tcatccgtca gcaccgccgc cgcataatca aacgccgcgc
    20761 ccatccgccc ggggtgcggc agcttcaccg catcgcacac tgcctgcgcc gtcccgtatt
    20821 gtgcggcgaa cctttctacg gtttcctgtt caaaagcaat ccattgcgcc tgatagaggc
    20881 cgtctgaatc gggaatatta ataacgtcaa acgtctgtcc gcctgccaac gcaaccgcct
    20941 cacccgccgc aatcctcaat tcgcgcctcg cccaccgcag cttcttgctt ccgcgccgca
    21001 cgataagcac agctggttca tataccgcca cgctgcggta caaggcggta tgatgttgca
    21061 cgatgccgcc caaagcaccc aatcgttcgc gcgtatgaaa gtaatttgac ggcactatta
    21121 tctccaacac gcagggccgc acataaggcg cagccctgtt taccctgatt tggaaggggt
    21181 tacttcccaa aaataaacca aaatcccact gccccgaatg gccgggtttc aaccatattc
    21241 cgtcggtgtg cctgcaactt gactccctcc cctgtggggg agggttgggg agagggtaaa
    21301 acggggcaga tacagacaat atttccgtta ccgccccgat gccctctccc tatccctctc
    21361 ccacgaaaga gggaatggat tgccgtttaa ataaaaaaat ctacataaaa atcaatgttt
    21421 tatagtggat taaatttaaa tcaggacaag gcgacgaagc cgcagacagt acaaatagta
    21481 cggcaaggcg aggcaacgcc gtactggttt gaatttaatc cactatatct caaacccaca
    21541 ttaggtctaa tcaaatggtc ggacatccat atccgacagc ccattttctt ttcagacggc
    21601 atttccagcc aacaagcgcg ccaatatccc ctcatacacc gccgacagct tcggaatatc
    21661 gtccagccgc acgttttcgt tgatttggtg gatggtcgca ttggacgagc ctaattcgat
    21721 gagttctttt gcaatggctt tgatgaagcg tccgtccgaa gtgccgccgg tggtggacaa
    21781 ttcggtctct acgccgcaga tttcggcaat ggcggcgcgt gccacgtcgg tcagtttgcc
    21841 cgcgtgggtc aggaagggct gccccgaaca cgaccactgc aaatcgtatt gcacaccgtg
    21901 tttgtccaaa atggcgtgga cgcgttgttt cagccctgct tcggtggact cggtggagaa
    21961 acggaaattg aatttgacat tcagctcgcc cggaatgacg ttggtcgcgc ctgtgccgcc
    22021 gttgatattg gaaatctgga aactggtcgg cgggaagtat tcgttgcctt cgtcccagac
    22081 ttcctgcgtc agctctaaca aggccggggc aaaagtatgg acgggattga ttgccaaatg
    22141 cggataggca atatgacctt gcttgccttt gacggtcagg ttgcccgaca gcgagccacg
    22201 ccggccgttt ttaatcatat cgcccaattt gcccacggcg gtcggctcgc cgacgatgca
    22261 gtagtcaatc aactcgccgc gcgctttcaa cacatcgacg actttggtcg taccgtccag
    22321 cgcgtcgccc tcttcgtcgg aagtaatcag gagcgcgatg ctgccttggt ggtcgggatg
    22381 ttcggcaaca aagcgttcgc aggcggtaac aaaacaggcg atgctggttt tcatatccgc
    22441 cgcaccgcgc ccgtataatc ttccgtcgcg ctcggtcggc tcgaacgggg gcgaatccca
    22501 tttttcaaca gggcctgtcg gcacaacgtc ggtatgccct gcaaaacaga cgacgggaac
    22561 tttcgtgccg cgtcgcaacc agatgttttt ggtgtcgccg aaatggagtt cttcagccgc
    22621 aaaaccgatt ttgtgcaggc gttcggccag cagttgttgg cagttttggt catctggcgt
    22681 cacggatggt cgggaaatca gctctttggc aagctctagg gattgagttt cggtcatatt
    22741 tgttcacttt tgaaattaga ccgtctgaaa cgttctgaat gtgattttca gacggcattt
    22801 aggttaggtt ggcatacggg gtgggtattt ttacccgtca gtcttctgaa tcatttgccg
    22861 tggcaggctt cgtaaagcgg cagcaaatct tccaccgttt ccgctatcca tttcgcgaca
    22921 tcctgcctgc ccaaatcgtc gcgttcgatg tgtttgccga tgcagaaaaa gtcttcgtcg
    22981 ttttgcaact ttcggtcgga ctcgttttgt tgcgcgacgg tgcggtagtc gtcgtattcg
    23041 ctttccgcac cgtgccacat atcgaaagaa gcgtattttt cggtatcaaa attatccaac
    23101 cagcggttgt aatcaggcag cgcgatgggc gacacgtcgg ctttgtagca gtgccaatcc
    23161 aagctgacgc tcaaacggcg gcggttgagc agtatcgaca aaatcgcggc ggaattttta
    23221 tattgttcgt atttgaagta ggcaaagaaa tgggcgcgaa cctgccagcc gttacaccag
    23281 cgttcgatgt gcggaggcgc aaacggcgca cccaattcgg cggcaacctg ctgaatcagc
    23341 tgctgccata tctgccagtt ttctttatag tcagccttga tttgcggaat gctttcaggc
    23401 tggtattttt taagctggga aaattggaaa aacgggatat tgaacaaatc gcaacttttc
    23461 ggggtcagca taatatatcc ttgagacgat tgtttcagac ggcattattt gcgccggcgc
    23521 gccgccataa tttcgccgat ttcggtcagt ttttcttttg ggataaaggt gttgcccata
    23581 tcaaacagcg gctcttcaat cgccagatgc acatcgtaac ctgccacaaa acgtttgaac
    23641 gcttcctcat cggggacata agcgttgtct gcttcgagtt tggcaaattc ggcagccacg
    23701 gcatcccagt tgccatacag gctcacatgc tgacgcaaaa gctcgtccac gctttcttgg
    23761 gcttgcggcg cgtattgcag cagcagcgga aagaagtttt cttcttcgtc ttcatggtgc
    23821 agcggcgcgg caacgttgaa ataccgggcg atttggcgga tggtttgcaa aacaatctga
    23881 ttgcagccgt tttcggcgat atagtccgac agcatggcga cttgtccgca aaaacggcgt
    23941 actttgccat ggcaggcata cagcatttca atcggttcgg caaaggtaac gcttttggtt
    24001 tcaaacggat tcatggtttt ttagatttta ttcttaaatt aacgtttcca gattataaca
    24061 aaacagcctg ctcaaggcag gctgtctgtt ttcaagcttt aggcgaatta gtcaaccaaa
    24121 gtaattttac cgttcatcaa agcaccgtga cccgggaagg tgcaggcaaa tttgtagtcg
    24181 ccgtcagcca atttggcagg atctagagtc agggaagact cttcgccgcc gccgatcagt
    24241 ttggtgtggg caacaacgcg cgcatcgtca ggtttaacgt agtcagtgtc agctgcgcca
    24301 acgccatctt tgaaaatacc gtccatgtct tcagttttac cgatgacaat gttgtgaccc
    24361 atgctggttt taggttgggt accggtgtgt ttcagggtga tggtgaattc cttacaagct
    24421 ttgcttactt ggatgtcttt agtgttgaac tgcatattgt cgttggattc gacagttgcc
    24481 gcacagttgc cggcagcagg ggcttcggca gcatctgcag gagcagcttc ggcggcaggc
    24541 gcttcggaag cgggtgcttc agcagcagga gttgcctcgg cagcaggcgc ggcaggttct
    24601 tgagagcagg cagccaaacc gataacggcg gcagaaatca gagccagata cgctttcata
    24661 acaaatctcc aatcgataaa ataatattcg gttttacaga aatcaaagtg caaccgccat
    24721 taacaaaacc ttgaaaaaga ttccgccgcg ttgcacaaac agatgtttcg gagcggcatt
    24781 ttgctacaaa tttcatttga aatcaaagcc tgtctgcaag tttacaatcg tttactttaa
    24841 aaggggcaat tttaccccga acctatttct ttagtattag acccattatc ctttacttct
    24901 taatattaac ggatgtttac acaaattccc gtatacattt tatgcgccat gccttctaac
    24961 caagtttgcc aatgcctccg ccaattcggg atgccgtttt tccaacttta ccgccgccga
    25021 accgaaactc tccagcgcag ccttactcaa atgcagggta ttggttttcg gcggtttttc
    25081 cggtttcggg gccagcctga ccgaaacaga gcgtatcgaa gcatcaagcc ctgccaactg
    25141 cggcaatacc gacggtgcaa tcattttcaa gcgcgatgcc gccatattgt ttgccgccaa
    25201 aaggacaagc ctgccgtctt cgatacatgc cgtctgaaaa tgcgggtgca ggttggcagg
    25261 cagcagtttt ttcacggcgg catccaaccg ccgccactgt cccgcctgtt tcaaaagtcc
    25321 ggaaagcagc gcgtcccgcc tgcccaactg ttccaaattc ataaaacata cacccaaaaa
    25381 gattgaaata ccgcaaacgc gcctttattt cagacggcat tagcactttg cacaaacgct
    25441 tgtgttaaaa tcgcgttttc gcccactatt atatcaggcg caggaattat tcatgctgac
    25501 aaacattgcc aagaaaatct tcggcagccg caacgaccgc ttgctgaaac aataccgtaa
    25561 atccgttgcc agaatcaacg cgctcgaaga acagatgcaa gccctaagcg atgctgatct
    25621 gcaagccaaa actgccgaat tcaaacaacg cctcgccgac ggtcagactt tggacggcat
    25681 tttgcccgaa gccttcgccg tctgccgcga agcgtcccgc cgcaccctcg gtatgcgcca
    25741 cttcgacgtg cagcttatcg gcggtatggt gctgcacgac ggcaaaatcg ccgaaatgcg
    25801 taccggcgaa ggcaaaaccc tggtcgccac cctcgccgtc tatctcaacg cgctggccgg
    25861 caaaggcgtg cacgtcgtta ccgtcaacga ctacctcgcc tcgcgcgacg caggcattat
    25921 ggagccgctc tacaatttcc tcggcctgac cgtcggcgtg attatttcag atatgcaacc
    25981 gttcgaccgt caaaacgcct atgccgccga tatcacctac ggcaccaata atgaattcgg
    26041 cttcgactac ctgcgcgaca atatggttac caaccaatac gacaaagtgc agcgcgaatt
    26101 gaattttgcc gttgtcgatg aagtggattc catcttgatt gacgaagcgc gcactccgct
    26161 gattatctcc ggccaggcag acgacaacat ccagttgtac caaatcatga acaccgttcc
    26221 gccccacctc gtccgtcaag agacagaaga aggcgaaggc gactattggg tcgacgaaaa
    26281 ggcacatcag gtcatcctga gcgaagcagg tcacgaacac gccgagcaaa tcctgaccca
    26341 aatgggattg ctggcagaaa acgactccct ctattccgcc gccaatatcg ccctgatgca
    26401 ccaccttatg gcggcattgc gcgcgcattc cctcttccac aaagaccaac attacgtcat
    26461 ccaagacggc gaaatcgtca tcgtggacga atttacaggt cggctgatgt ccggccgccg
    26521 ctggtccgaa ggcctgcatc aagccgtaga agccaaagaa ggcgtggaaa tcaaacgcga
    26581 aaaccaaacc cttgcctcca tcaccttcca aaactatttc cgcctgtaca ccaagctctc
    26641 cggtatgacc ggtacggcag ataccgaggc tttcgagttc caaagcatct acaacctcga
    26701 aaccgtcatt atcccgacca accgccccat acagcgcaaa gacttcaacg accagatttt
    26761 ccgttccgcc gaagaaaaat tcgaagccgt cgtcaaagac atcgaggaat gccacaaacg
    26821 cgggcagccc gtcctcgtcg gtacaaccag catcgaaaac tccgaactgg tctcccacct
    26881 tctgcaaaaa gccggactgc cgcacaacgt cctcaacgcc aaagaacacg aacgcgaagc
    26941 actgattgtc gcccaagccg gcaaagtcgg cgcgattacc gttgccacca atatggcggg
    27001 acgcggtacg gacatcgttt taggcggcaa cctgaagcac caaaccgatg ccatccgtgc
    27061 cgacgaagcc ttgagcgacg aagagaaaca ggcacaaatc tccgcactcg aagacggctg
    27121 gcaggcagaa cacgacaaag tgatggaagc aggcggtttg cacatcatcg gtacggaacg
    27181 ccacgaaagc cgccgtatcg acaaccagtt gcgcggacgt tccggccgtc agggcgaccc
    27241 cggatccagc cgtttctacc tctcgtttga agacccgctg ctgcgcctat tcgcactcga
    27301 ccgcgccgcc gccatcctca accgcctcgc ccccgaacgc ggcgttgcca tcgagcacaa
    27361 cctgctgacg cgccaaatcg aaggggcgca acgcaaagtc gaaggcagaa acttcgatat
    27421 gcgcaaacag gttctggaat acgacgacgt cgccaacgag caacgtaaag tcatttacag
    27481 ccaacgcaac gaaattttaa ccagcaaaga catcagcgac ctgatgaagg aaatccgttc
    27541 tgatgtcgtc agcgacctcg tggataccta tatgccgccc gacagcatgg aagaacaatg
    27601 ggacatcccg actttggaga accgtctggc tgccgaattc agactgcacg aagacatcca
    27661 atcctggctg aaggcggaca atgcgattga cggtcaagac atcaaagaac gcctgatcga
    27721 acgcatcgaa aacgaatatg ccgccaaaac cgaactggtc ggcaagcagg caatggccga
    27781 tttcgagcgc aacgtgatgt tgcaggtcat cgacaaccaa tggcgcgaac acctcgccgc
    27841 tatggactac ctgcgacaag gcatacacct gcgcagctat gcccaaaaaa atccgaagca
    27901 ggaatataaa cgtgaagcct ttaccatgtt ccaagacctg tggaacggca tcaaattcca
    27961 tattgcctcc ctgcttacct cggttcaaat cgaacaaaac cctgtcgcgg tggttgaaga
    28021 gcaacccatc ggcaacatcc agtccatcca ttccgaatcg cccgatatgg aagaactttt
    28081 gggtcagtcg caaaccgatt tggttaccga agcctttaat cccgatggga cagatttcag
    28141 ccccgaagcc ttggaagcgc gggggcaaat cgtccaccgc aacgacccct gcccctgcgg
    28201 cagcggtttg aaatacaaac aatgccacgg caaactggct taagcgtttg aacgcaaatg
    28261 ccgtctgaac atcccgctcc cgtttcagac ggcattttgc ctgaaccgcc acatccgact
    28321 gccattccga aaaatcccga tttcgtaccg tccgtaccaa aaacagacat cccgtccgcc
    28381 ccacatcatg attccatccg acttcattga cgagctttta gccaaaaccg atattgtcga
    28441 tattatcgac gagcaggttc cgctgaaaaa aggcggggcg aactatatgg cgtgttgccc
    28501 gttccacaag gaaaaaacgc cgtcgttttc ggtcagtcca accaagcagt tttaccattg
    28561 tttcagttgc ggggcgcatg gttcggcgat tggttttgtg atggagcatc aggggctgtc
    28621 gtttccggag gctgtgcagt ttcttgccga ccgtgtgggc atggttgtgc ctaaagtgcg
    28681 tgggcagaac gataatcccg aagtccgtgc cgaacgtaag aaaaaaaagc agacgctgga
    28741 agaaacgacg gcggcggcgg ctgattttta cgcgcaacag ctaaaattca atccggcggc
    28801 aaaagcttat ttggacaagc gcggcttgag tgcagaagtt atcgcgcatt atggtttggg
    28861 ctacgcgccc gacggctggc agcctttggc gcaagtgttc caaccgtatc cgaataccgc
    28921 gttggtggat acggggatgg tgattgacaa tgaggggcgg tattacgacc gcttccgcca
    28981 tcggattatg ttccctatcc gcaatccgcg cgggcaggtc atcggtttcg gcggcagggt
    29041 gctggacgac tccaaaccca aatatctaaa ctctcccgat acacctttgt ttgacaaagg
    29101 gaaaaacctt tacggcttgt atgaggggcg tgccgctgtc aaggaagcgg agcggattct
    29161 ggtggtcgaa ggctatatgg acgtggtcgc gctggcacag ttcggcgtgg gctacggtgt
    29221 ggcggccttg ggcacggcaa caacagctga acacgtcaaa atcctaatgc gtcaggcaga
    29281 cagtatttat ttctgtttcg acggcgacag cgcggggcga aaagcggctt ggcgcgcgct
    29341 ggaaaacgcg ttgccgcagt tgaaagacga caaatcgctg cattttttgt tcctgcccga
    29401 ggaacacgac cccgacagtt acatccgtgc ctacggcaaa gcgcaatttg aagacgcgct
    29461 tctgaatcaa agcaagcctt tgtcggagta tttctgggaa cacctttcag acggcattca
    29521 tctcaatacg caggaaggca aggcggaatt ggtgaaaacc agttcgccgc ttttggcgca
    29581 gattaccgcg ccggcattgg cttatttgtt aaaacaacgg cttagcgaac tggtcggcat
    29641 cgaccccgac aacctcgcgc aactgctagg acaggaagcg ccgaagcggc acgtcaaaca
    29701 aaaaaactac aaactgcctc cgatttccgt caaacagccc gtcatgctga cgctggtaca
    29761 gcggcaaatc cgcagcctct tgataaatcc ggattgggct gcatatatag acctgcccga
    29821 ttatctggcg ttggacggtg atttcgcctg ccttgccaat cttgccgaat cgattaaaaa
    29881 ccatgccgcc gtacccgaaa ccgctcaggt tttagagtat atgcgcggct cgccttacga
    29941 agaaacgata acccgaatct tccattcaac gcaccaatcg gaagaaatga acagcagcag
    30001 tgaagaagat tgcgagaatt tccaaatcgg catgaaaaaa ctgctcaatg agttaaaata
    30061 cagccaaatc gaagcattga aacaaaaaag cctgcaatcc ggcttaaatg aaagcgagaa
    30121 aaaacttttg ctgtcgttgc tgaccgcaaa acaaaattga ccggcggatt ccgccacccg
    30181 taaacccgtt atgccgtctg aaaagcactt accccggctg caacaacgac acctgcagaa
    30241 cacccatccc aaaaagcctt cagacggcat cagagtaccc gctctgccac gccttcaggt
    30301 gcgttcaaac gcaaaccgtc ggcaccttac caacagaaag cagacaatgt ccagaaacca
    30361 aaatcacgaa gaatatcaag acgacacccg tccgttaagc attgaagagc aacgcgcgcg
    30421 cctgcgtcag ctcatcatca tgggtaaaga acgcggctac atcacctact ccgaaatcaa
    30481 cgacgccctg ccagacgata tgtctgatgc cgaccaaata gacaatatcg tcagcatgat
    30541 ttccggtttg ggcatccaag ttaccgaaca cgcccccgat gcggaagaca tattgttaag
    30601 cgacaatgcc gccgttaccg acgatgatgc cgtcgaagaa gccgaggccg ccctttccag
    30661 tgcagattcc gagttcggca gaaccaccga ccccgtccgt atgtatatgc gcgaaatggg
    30721 gcaggtcgac ctgctgaccc gcgaagacga aatcatcatc gcaaaaaaaa ttgaaaacgc
    30781 cctgaaaaat atggttcagg ccatctccgc ctgcccggga tccattgcgg aaatcttaga
    30841 actcatcgaa aaaatccgca aagacgaaat ccgcgtcgac gaagtcgtag aagccattat
    30901 cgacccgaat gaagtattgc tcaacgaatt gggcttgggg cacttggaaa ccacagcgcc
    30961 cgagaaacct tccaacgaca attcggacga aaacgaagac gacgaagaat cggaagaaga
    31021 tgcggatgaa atctcggcag ccaatctcgc cgaattgaaa caaaaagtca tcggccactt
    31081 tgcccaaatc gaaaaagact acaaaaaaat gattggccgt ttggaaaaac accacagccg
    31141 gcacaaagac tatctcgcct accgcgacgc gattgccaac aaactgctgg aagtccgttt
    31201 cgccacccgg caaatcgaca gcctcagcag cagcctgcgc gggaaagtag aaaacatccg
    31261 caaactcgaa cgcgaaatcc gcgacatctg cctcgaccgc gtccatatgg aacgcgacta
    31321 cttcatccaa aacttcctgc ccgaaatcac caatctagaa tggattgaag aagaaatcgc
    31381 caaaggcagg gtttggagcg acgcgctcga ccgcttccgc cacgccatcc tcgaaaaaca
    31441 aaccgagttg gcggatatgg aaaaagaaac ccgcatttcc atcgaagagt tgaaagaaat
    31501 caacaaaaat atggtgtcaa gcgaaaaaga aaccgcagcc gccaaacagg aaatgattca
    31561 ggcaaacttg cgcctcgtga tttccatcgc caaaaaatat accaaccggg gcttacaatt
    31621 ccttgatctg attcaggaag gcaacatcgg tttgatgaaa gcggtcgata agttcgaata
    31681 ccgcagaggc tataaattct ccacctacgc aacctggtgg atccgccagg caattacacg
    31741 ctcgattgcc gatcaggcgc gtaccatccg cattccggta catatgattg aaaccatcaa
    31801 caagatgaac cgcatctcgc gccaacacct tcaagaaacc ggcgaagaac ccgattccgc
    31861 caaacttgcc gaactgatgc agatgcccga agacaaaatc cgcaaaatca tgaaaatcgc
    31921 caaagagccg atttcgatgg aaacccccat cggcgacgac gacgattcgc acttgggcga
    31981 cttcatcgaa gatgccaaca atgttgcgcc ggccgatgcg gcaatgtaca ccagcctgca
    32041 cgaagtaacc aaagaaatcc tcgaaagcct gacaccgcgt gaggcaaaag tcctgcgtat
    32101 gcgtttcggc atcgatatga acaccgacca cacgctggaa gaagtcggca gacagtttga
    32161 cgtaacgcgc gaacgcatcc gacaaatcga ggcaaaagca ctccgcaagc tgcggcatcc
    32221 gacaagaagc gaccgtttga gaagtttctt ggacagcgaa gacagcaagc tgtaaaccaa
    32281 aaaaccgcag gtttcaaata cctgcggttt tttcttacac aataaacaac gcttccacat
    32341 atcccacact cctatcccga gacctttgca aaatccccca aaatccccta aattcccacc
    32401 aagacattta ggggattttc catgagcacc ttctttcagc aaaccgcaca agccatgatt
    32461 gccaaacaca tcgaccgctt cccactattg aagctagatc aagtgattga ttggcaaccg
    32521 atcgaacaat acctgaaccg tcaaagaatc cgttacgtcc gagaccaccg cggccgtccc
    32581 gcctacccgc tgttgtccat gttcaaagcc gtcctgctcg gacaatggca cagcctctcc
    32641 gatcccgaac tcgaacacag cctcatcacc cgcatcgatt tcaacctgtt ttgccgtttt
    32701 gacgaactga gcatccccga ttacagcacc ttatgccgct accgcaaccg gctggcgcaa
    32761 gacaataccc tgtccgaatt gctggaactg attaaccgcc aactgaccga aaaaaaccta
    32821 aaaatagaga aagcatccgc cgccgtcatt gacgccacca ttattcagac cgccggcagc
    32881 aaacagcgtc aggccataga agtcgatgaa gaaggacaag tcagcggcca aaccacaccg
    32941 agtaaagaca gcgatgcccg ttggacaaag aaaaacggcc tctacagact cggttacaaa
    33001 caacataccc gtaccgatgc ggaaggctat atcgagaaac tgcacatcac cccagccaat
    33061 acccatgagt gcaaccacct gttgcctttg ctggaaggca ttaccgaagg cacgaccatc
    33121 tatgccgaca aaggctatga cagtgcggaa aaccgaacat ctggaagaac atcagttgtt
    33181 agacggca
//
LOCUS       DAWFLT010000015        32965 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWFLT010000015 DAWFLT010000000
VERSION     DAWFLT010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32965
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.015"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(338..4507)
                     /locus_tag="WGR74_000692"
     CDS             complement(338..4507)
                     /locus_tag="WGR74_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ0905162.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGSFVIRQDGTVHIGNTSVALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGTLKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNSKGK"
     gene            complement(4562..6409)
                     /locus_tag="WGR74_000693"
     CDS             complement(4562..6409)
                     /locus_tag="WGR74_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ0905163.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLHSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKAYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGAFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNANVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6974..8203
                     /gene="sstT"
                     /locus_tag="WGR74_000694"
     CDS             6974..8203
                     /gene="sstT"
                     /locus_tag="WGR74_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ0905164.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAYTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8315..9808
                     /locus_tag="WGR74_000695"
     CDS             8315..9808
                     /locus_tag="WGR74_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0905165.1"
                     /translation="MFKRSVIAMACIVALSACGGGGGGSPDVKSADTLSKPAAPVVTE
                     DVGEEVLPKEKKDEEAVSGAPQADTQDATAGKGGQDMAAVSAENTGNGGAATTDNPEN
                     KDEGPQNDMPQNAADTDSSTPNHTPAPNMPTRDMGNQAPDAGESAQPANQPDMANAAD
                     GMQGDDPSAGENAGNTADQAANQAENNQVGGSQNPASSTNPNATNGGSDFGRINVANG
                     IKLDSGSENVTLTHCKDKVCDRDFLDEEAPPKSEFEKLSDEEKINKYKKDEQRENFVG
                     LVADRVEKNGTNKYVIIYKDKSASSSSARFRRSARSRRSLPAEMPLIPVNQADTLIVD
                     GEAVSLTGHSGNIFAPEGNYRYLTYGAEKLSGGSYALSVQGKPAKGEMLAGTAVYNGE
                     VLHFHTENGRSYPTRGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGN
                     WTENGGGDVSGRFYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9884..10099)
                     /locus_tag="WGR74_000696"
     CDS             complement(9884..10099)
                     /locus_tag="WGR74_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905166.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDDGFIN"
     gene            complement(10092..10307)
                     /locus_tag="WGR74_000697"
     CDS             complement(10092..10307)
                     /locus_tag="WGR74_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ0905167.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(10382..11725)
                     /gene="argG"
                     /locus_tag="WGR74_000698"
     CDS             complement(10382..11725)
                     /gene="argG"
                     /locus_tag="WGR74_000698"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ0905168.1"
                     /translation="MSQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRTQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIKPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAITGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLALGEGSVLPQLGNKK"
     gene            12052..12192
                     /locus_tag="WGR74_000699"
     CDS             12052..12192
                     /locus_tag="WGR74_000699"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905169.1"
                     /translation="MQTAALKQTLPEKRKLDPLPNPLPWEREQITAPSTFSDGIGKET
                     HL"
     gene            12189..12989
                     /locus_tag="WGR74_000700"
     CDS             12189..12989
                     /locus_tag="WGR74_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ0905170.1"
                     /translation="MNAFNLTIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAAALDLPVVRHPEAAKFIEG
                     VTRKRGEPLDSPEHAQRLKMADFPQGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLDTYYVGRFNQTKRGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            13029..14015
                     /locus_tag="WGR74_000701"
     CDS             13029..14015
                     /locus_tag="WGR74_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ0905171.1"
                     /translation="MQYRIRRENEAPEAKNTGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            14672..14934
                     /locus_tag="WGR74_000702"
                     /pseudo
     CDS             14672..14934
                     /locus_tag="WGR74_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021111752.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(14972..15346)
                     /locus_tag="WGR74_000703"
                     /pseudo
     CDS             complement(14972..15346)
                     /locus_tag="WGR74_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15519..15875)
                     /locus_tag="WGR74_000704"
     CDS             complement(15519..15875)
                     /locus_tag="WGR74_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ0905172.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITESQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(16018..16362)
                     /locus_tag="WGR74_000705"
     CDS             complement(16018..16362)
                     /locus_tag="WGR74_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905173.1"
                     /translation="MNLENYENILIKLLFYHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDLLPD
                     NMIINNFFEIND"
     gene            complement(16521..16921)
                     /locus_tag="WGR74_000706"
                     /pseudo
     CDS             complement(16521..16921)
                     /locus_tag="WGR74_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16925..17665)
                     /locus_tag="WGR74_000707"
                     /pseudo
     CDS             complement(16925..17665)
                     /locus_tag="WGR74_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(17735..18052)
                     /locus_tag="WGR74_000708"
     CDS             complement(17735..18052)
                     /locus_tag="WGR74_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905174.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(18049..18660)
                     /locus_tag="WGR74_000709"
                     /pseudo
     CDS             complement(18049..18660)
                     /locus_tag="WGR74_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253822.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(18744..19046)
                     /locus_tag="WGR74_000710"
     CDS             complement(18744..19046)
                     /locus_tag="WGR74_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905175.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPIAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSIDKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(19225..19431)
                     /locus_tag="WGR74_000711"
     CDS             complement(19225..19431)
                     /locus_tag="WGR74_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905176.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            complement(19397..20146)
                     /gene="mafB"
                     /locus_tag="WGR74_000712"
                     /pseudo
     CDS             complement(19397..20146)
                     /gene="mafB"
                     /locus_tag="WGR74_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257312.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(20572..20916)
                     /locus_tag="WGR74_000713"
     CDS             complement(20572..20916)
                     /locus_tag="WGR74_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905177.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(20954..21298)
                     /locus_tag="WGR74_000714"
     CDS             complement(20954..21298)
                     /locus_tag="WGR74_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905178.1"
                     /translation="MFLDDVNVFLDDLNVFLDDLNTNPITDEWYMSNFADKHIKILES
                     YEAFDILKQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQ
                     EISQDILNEIFR"
     gene            complement(21301..21489)
                     /locus_tag="WGR74_000715"
     CDS             complement(21301..21489)
                     /locus_tag="WGR74_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ0905179.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENSRTGEKTDFKFKD"
     gene            complement(21559..21894)
                     /locus_tag="WGR74_000716"
                     /pseudo
     CDS             complement(21559..21894)
                     /locus_tag="WGR74_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(22000..22371)
                     /locus_tag="WGR74_000717"
     CDS             complement(22000..22371)
                     /locus_tag="WGR74_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0905180.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            complement(22368..22619)
                     /locus_tag="WGR74_000718"
     CDS             complement(22368..22619)
                     /locus_tag="WGR74_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905181.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            complement(22751..22957)
                     /locus_tag="WGR74_000719"
     CDS             complement(22751..22957)
                     /locus_tag="WGR74_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905182.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(22967..23812)
                     /gene="mafB"
                     /locus_tag="WGR74_000720"
                     /pseudo
     CDS             complement(22967..23812)
                     /gene="mafB"
                     /locus_tag="WGR74_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(23909..24121)
                     /locus_tag="WGR74_000721"
     CDS             complement(23909..24121)
                     /locus_tag="WGR74_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905183.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            complement(24197..24556)
                     /locus_tag="WGR74_000722"
     CDS             complement(24197..24556)
                     /locus_tag="WGR74_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0905184.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            complement(24561..25277)
                     /locus_tag="WGR74_000723"
                     /pseudo
     CDS             complement(24561..25277)
                     /locus_tag="WGR74_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(25494..25877)
                     /locus_tag="WGR74_000724"
     CDS             complement(25494..25877)
                     /locus_tag="WGR74_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905185.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLGRI
                     TTNECINGIKKIYQKNKLEFDNILK"
     gene            complement(25843..27288)
                     /gene="mafB"
                     /locus_tag="WGR74_000725"
     CDS             complement(25843..27288)
                     /gene="mafB"
                     /locus_tag="WGR74_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ0905186.1"
                     /translation="MKLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNARGSVKNRVYAVQTFDATAVSPVLPITHERTGFEGIIGYETHFSGHGHE
                     VHSPFDNHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WANRMDDIRGIVQGAVNPFLMGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAAATALQDSAFAVKDSINSARQWADAHPNITATAQTALSVAEAAGTVWRGKKV
                     ELNPTKWDWVKNNGYKKPAVRLMQTVDGEMAGGNKPPKPSTQQHSTHSDNNIGLPAPY
                     VKPDTSISPTGTIYVDSKGNAIPTPIGGYITGSPDARFIQVRDSNGNLTGARIDDAHH
                     ANTHQDPRALTGHAHVPGVTNDDGTPWLPIK"
     gene            complement(27328..28290)
                     /gene="mafA"
                     /locus_tag="WGR74_000726"
     CDS             complement(27328..28290)
                     /gene="mafA"
                     /locus_tag="WGR74_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0905187.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPARSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVETDNSHEGYGYSDEVVRQHRQGQP"
     gene            complement(28474..29193)
                     /locus_tag="WGR74_000727"
     CDS             complement(28474..29193)
                     /locus_tag="WGR74_000727"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ0905188.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            29289..29396
                     /locus_tag="WGR74_000728"
                     /pseudo
     CDS             29289..29396
                     /locus_tag="WGR74_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(29518..30372)
                     /locus_tag="WGR74_000729"
     CDS             complement(29518..30372)
                     /locus_tag="WGR74_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ0905189.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(30501..31229)
                     /gene="rpsB"
                     /locus_tag="WGR74_000730"
     CDS             complement(30501..31229)
                     /gene="rpsB"
                     /locus_tag="WGR74_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ0905190.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDHRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(31430..31639)
                     /locus_tag="WGR74_000731"
     CDS             complement(31430..31639)
                     /locus_tag="WGR74_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905191.1"
                     /translation="MWHIVAIGYLFVAVMFSAAQPSIARALIYLVFWAVLPTVFTVFV
                     VTIRRRNRLMKQQEQAEFEQQRAQR"
     gene            31717..32298
                     /locus_tag="WGR74_000732"
     CDS             31717..32298
                     /locus_tag="WGR74_000732"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ0905192.1"
                     /translation="MRNEEKHALRRELRGRRSQMGWDVRAAATVKINRLLKRYIKHGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     ARLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVDRLSVEAHDLPLDGFVSEAGILCF"
     gene            32359..32949
                     /locus_tag="WGR74_000733"
     CDS             32359..32949
                     /locus_tag="WGR74_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0905193.1"
                     /translation="MSERIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT         7951 a         7546 c         8048 g         9420 t
ORIGIN      
        1 gcgggaatga cggaaagtgg cgggaatgac ggttcgggca ttccttaaat tacctgtgta
       61 tcgttgtaaa tcttagagat ggcggaatat agcggattaa caaaaaccag tacagcgttg
      121 ccttgccttg gctcaaagag aacgattctc taaggtgctc aagcaccgag tgaatcggtt
      181 ccgtactatt tgtactgtct gcggcttcgt cgccttatcc tgatttttgt taattcacta
      241 tatcgattct gttcccttgc aggcggacgg cggcggcgga cggtggaaaa gcaggttttc
      301 aaatgtccgg tttggcggcg cgccgtctga aaaccgctta ttttcctttg ctgtttccgg
      361 cggagtcttt tttgtccgaa ccggagaagc ggtcgaaacg tatggtgtat gtcagctcgc
      421 cgcccgacga acggctgccg atacgggcaa ccgcctgtat ggcgcgggtc agccggtaaa
      481 tcagtttgac ggactgttcc gcgctggaga tgctgtattc gtagccgatg tagagtttgc
      541 cggtcagttg tttgccgacg gtcagcacct gttcggcggg gttgagttcg ccggtttgcg
      601 cgttgcggct gcgcttgctg gtaaagccta aatcatccac cagcccgatg cggtcgttga
      661 tttgcccggc aagcagcgcg ccggcggctg cggacagggc ggcattgtcg ccgctgctgc
      721 cactgccggc gcggttgagg atgagccagg agagcttgtc tttttcactc atcggttcgt
      781 ttgccgtcag cgtaatgcgc ggactgttga ggctgcccaa tatttccacg cccgcaccga
      841 cgggggaaag gcggcgttcg gcgcggatgt tgaggttggg gtcgttgagc gggccgacaa
      901 aggagaccgt gcctttggta atgtccaaat cctgcccgta tgccttataa cgccctttga
      961 tgacgcggac cgtgcccacg ccccgcacgc ttccgcccga ttgggcggtc agggtcagtt
     1021 tgccgcctat ggtaacgtcc gcgccgtagc cggcgaagcg gatgccgtca ttgaggtcta
     1081 aagtcaggtt catattgacg gggagcggtg ccgccgcctc ttttttgact tcgcctaata
     1141 cgacgacatc gtcgccgacg gacggcatcg aggatttttg cgaaccgaac agcccctgat
     1201 ccgttttaat catcccggta acggatatgc ctttttgcgg cgaatagcgc aggcgggtgt
     1261 tgccggaaac cgtcaggcgg cggttggggc gggacaggat gcggtatttg tcgaacaccg
     1321 cgccgatatc gacatcgggt ccgctgtttt ccataccgac cgtaccggag agttccgccg
     1381 tcccttcgtg ccggaatttc aggctgtcga ttacccattt cctgcccgcg atatgcgaac
     1441 gcagcgagcc gttgtccaag atgatgcctt gggtttggtt gcggtaatag agtttgtcgc
     1501 cgttgatgct gccgcccaag tgcggatcgg cgatgctgcc gccgagggtt acggcggcat
     1561 tcaggctgcc tttgacggtt tgtccgaccg gtaggaagtt gcggaatact tcggcatcgg
     1621 caacggtcag gttgagcctg ccgcccaaag gcgcggtatc gaaagagcgg ctttgcccga
     1681 cggtgatgtt gccgttgatt ttcccgtagt tgctgctgcc gttgacggcg gcattgacgg
     1741 acggagagcc gacccgtccg ccgatttgcg cggcggcatt caggctgccg gtaatgtttt
     1801 gcgcggcggc gggcagaaag ggcttcaatg tgcccaagtc gggaagggag gcggtaatcc
     1861 tgccgccgag cggtgcattt gccatattgc cgccgaaggc gttgccgatg tccaaatcgg
     1921 cgttaatccg cccgaaacgc gcgccgccgt caagcaggat tccgatacgg tcgttttgaa
     1981 agcgcgtttt cagggaaaat gcgttcaaac ccaaagcctg cccgccgggc aatacggcat
     2041 cgccgctttg ccggctgata ttgaggtagc cgcgcgcgtt tcgcccgtag gcgacatccc
     2101 agtcgccgtt taaaaccaga ttgtgttcga agggcggttt gaagaaattg tgcaactcgg
     2161 cgatatgcag accgtgtgcg ccgccttttg ccgatatgcc ggttttttta tcccaagaaa
     2221 agtgttgcag gttgaggctg ccgcccattg cctgccaatt tgccgcactt gccgccacgc
     2281 gttccgcacc ggcttcgagc gtcatacggt tttgcagctt gaggttgaat gcgccgccga
     2341 tgtcgaggat gccgatgctg cctttccatc gggtaagttc cctgttgatg ccgcctgaag
     2401 cgtccaaatc gaatttgaac ggtttgccat ccagcgtcat ggcggcgtgt gtgcggatgc
     2461 ggtgctgcac gcccgtgccg tccagcatca ggtcggcggt atcgacaacc gccgctccgc
     2521 ccgacagcga aaggcggctg cctttaatgt cggcgcgtat cgggcggctt gtgtcgggcg
     2581 aacctttgag cgtgaaatcg agcgaacgga tgtctgccgc cttgccgatg tgcaggttgc
     2641 gcgccgcgcc ggaaaggtcg gtttcaaagg ttcggatgcc accgtccaaa tcgccggaaa
     2701 ggtgtccgcg tacatttaaa gaccccgcga gtccgaaacc gaaacgggat aaatcgggtg
     2761 cggtgatatt gaggttaagc cggtcgcctt ttttgccgaa gccgccgtct gttttaataa
     2821 tgttccgccc cagccgcaaa tcgacggcgg cacgcggaag gtggcgggac tcgtaaacaa
     2881 tgtcggcact gccggcaatc ggtacgccgt tgaacgtgcc gggtaaaaac cgcattttgc
     2941 ctgtgaattt ttcttttgcc agttcgccgg caaggtttat tgagccgttg atattgcctg
     3001 ccggaagttg cggatcgatg cgcgaagggt cgaatgcgcg ggaacggatg tccagcttga
     3061 gcaggcggtc tttaaacagc tcgagatagc cttgcgcggt caggctgcct tgcccggcgg
     3121 cgatgttgac ggtgtcgagc accagtttcc gctgtccgtt tgcggggtcg cttgcaatgg
     3181 cgaggctgcc gtccgtgcgc gccgtgccga tgccgagttg ccaagagatt ttgggcgagg
     3241 cggtcgtgcc accgatgccg atgctgccgt ccaacctgcc tttgaacgcg gtttgcagta
     3301 cgtcttccgc gccgacggag ttgatgccta tatttaaatc gaggatgtct ttttcggtgt
     3361 cgattttgcc cgacagcctg atgccgcccc gtccgagcag ggcgacggac gtattgccga
     3421 tatgcaccgt gccgtcctgc cggatgacaa agctgcctaa aacctgacgg acggggatgc
     3481 cgttgcggtc ggcaaagccg gctttggtgt tttccaaatc gagcgagcct tccagcgcga
     3541 tgccgtctga aaacgacggg atggcggtca ggtcgaaatt cagcccggca tcgggcaggg
     3601 aaggcacgaa ggcggacgga ttgatgttga accctttgac cagtacttct tccaatgttt
     3661 tatccaatga ttcggcaaac gggtggatga cggattttcc cgagaggcgg atattgccgc
     3721 cgtcgatcgc cagttcggcg cgcacatcct tcaggctgcc gctcagccga gccgtactgt
     3781 gtatggtttt gccttcgagt ccgcctttgg tgtaaatggc ggtatcgagg gcaaacggtt
     3841 ttttcaagcc gaccgaggct gaccccgacg aactgctcca cggcgtgtcg gcagccttca
     3901 ggtcgaggcg gtgtcctttg cggtcgtaac ggtatgaagc atccagccgt tcgagataga
     3961 cggtttgttt gtcaaaggct ttgcccatgc tgattttgcc cgtctcgaag cggtcgagat
     4021 agacggcggc aggcaggtct atgctgtcgg gaaggctgag cggcgggcgt tcttctttag
     4081 gcggagtcgg tttggtaacg atggcgatgt cgccggcgga aatttcggta atgtgcaggc
     4141 tgcggcgcat cagttcggac ggtttccacg cgaagcggaa gcggctgatt ttaaggtctg
     4201 ccccctcggt ttctatcgac cagttgtcgc cgtcgaagcc gtcgagcagc gtgcctttga
     4261 ggttttggga ggaaatgttt acgccgaacc aagacgggat ttggtacagc ccgaagcgca
     4321 aacccgcttc cgtgccggcg agccagccga ggaaacatac tgccaaaatc agaacagaca
     4381 gcagtgccgc cgacagcttc agcagcgggc ggcgtttttt tgccggcggg gtagggcggt
     4441 gttcagacgg cattttgcgc gtgccgtttt cggtcggatc ggtatctgtc ggtgcggtat
     4501 cggtcataat gtctgtttca aaatggtttg ctgcaatgcc gtctgaagcg gccgtatcgg
     4561 tttagaagcg cgttcccaag ctgatgtgcc agcggatttt cttgtcgctg tgcccgtagg
     4621 cgatgtcgaa ggaaaacggc gcgagcgggc tgaaccagcg cacgcccagt cccgaaccgt
     4681 gtttcagctt catacgtttg aaattggcgg cggcgtcgcc catatcgtgg aacaccgcgc
     4741 cggaaagggt gcgcgtaaac ggcagttggt attccaggct gcccaccaag agggcgcgtt
     4801 cgggcaggac cgatccgttc gggccggcaa gcccgatgct gtcgagttcg taaccgcgca
     4861 cggaagacgc gccgccgctg cggaacatca gccctgaagg aacgttggca ttgtcgcggg
     4921 caacggtgta acccgcttgt ccgcgtatga tgaacgtgcc gagttttttg ttttcgggcg
     4981 tgaagaaata acctgcgcgg gcagaggtgc ggatcagcgc ggtggaggac aggaatgcgc
     5041 ccaaagtcgt accgattttg ccgtcgaggt aatggccgtt ttcgggatgc agcacgttgt
     5101 tgagcagctg gcgtttccaa gaggcggtca gcatcgtggc gtggctgttg cccaaatcga
     5161 tatccgagcc ggggattttc cggccttctg cgagaaactc cgcccccagc ctggcatcga
     5221 tgcccgcgcg gtcgcgcaca taccagatgc cgccggagaa ggcgcgtttt tcgaggtttt
     5281 gggtggtcga acggttgtag gaaacgttgc ttgtccagta gttgccccga tagttgcgcg
     5341 gctggctgat gccggcggca agcgtggttt cgtatttgtc catatcccag acgaccgaac
     5401 cgatatagcc tttgttgaag aggttgtaat agtcgtaggc gattttgccg cccaaaccgt
     5461 attccgaatc gaggcggatg ccggtttcga gcttgtggcg tttgacctcg gttacgctga
     5521 ctttgacggg gacgcggtcg ccttggaggc ggtcgaagtc ggcttgtacg gacgcgccgg
     5581 aataatgccc gttttgttcg agcgcctgtt ggaagtcgag cagcaggtcg aggtcgtagg
     5641 gcgtgcccgg ttggaagcgc gccaagccgg agacgatttg ttcggggtaa cgctgcgtgc
     5701 cggtaatttc aaagtcgccg aaggcgatgg ggcggccgct gtccacgacg acgttcaaat
     5761 cggcggtggc ggtatcgggg ttgacggccg cccgggtgtt gccgagcttg gcaagcgggt
     5821 aggctttgcg cgttaccgcg ccgaggacgg aagttttgct gttttcccaa ctgtcctgat
     5881 cgaaatcact gcctaccggc tgctgccagt tttccagcgc gttgcggtag tattcggcga
     5941 ggttgccgtc tgaaaggatg tcgccgagga tggcgacgcc gacgttggcg attttggtgc
     6001 gcgggcccgg tgtgatgtgt accgtataag ctccgtcttt ttccgtcagg ctgactttgc
     6061 tgctgaaata gcctttgctg tggagcattg ttttaacgtt gtccggtgct tcttcggcga
     6121 ggaagcccgt ctgttccttg tccaatactt cttcctgctg ctgcgtgatg agcggcaggt
     6181 gttcttcgac catatctttg atttcactat cctgcgtgtc gatgcggacg gggaatttgg
     6241 gttttaattt aactgattcg gtgtcggggc ttttgttttt gaacaatgcg aaacccgccg
     6301 ccttgttttc ggaaaggtcg gcggcaggcg cgtatgcgtg cggaaagaaa aataaagccg
     6361 gcaggagcag ggcggtcggt ttaatcatca tggtacgggt gtcgtgcatc ggtgtggcgg
     6421 ttttcgattc taacactgtt cggacagggt ttcgaacggc cgtttttgtg ttgttcgtac
     6481 ggggcgggac gcttcagacg gcatactgcc gccggtattt cggaggttgc ggcccgccgg
     6541 aaatgtatct gcttgtttta aggtattttg tttctaatcc gcatatggaa aacaggctgc
     6601 gtttcggaat agtgtcggaa aataaacggg attaaataaa tttattttcg ttcaataaat
     6661 taaaccgtcg cgccgaatga taaagcaaag cctgtccggc actgcatagg gcaaatcaac
     6721 gcaaccttca tagtggatta acaaaaacca gtacggcgtt ggctcgcctt agctcaaaga
     6781 gaacgattct ttaaggtgct gaagcaccaa gtgaatcggt tctgtactat ttgtactgtc
     6841 tgcggcttcg tcgccttgtc atgatttttg ttaatccact ataaaacaca gccgaaaaat
     6901 aaaaaaaaca cagagaatga gttttctttt aaaatacaac attttttaac atatcacaac
     6961 ataaggaaga attatggcgt tcggcaaatc cttatttcat gcgatcggcc gcgtcagcct
     7021 ggtcaggcag attgccgccg gtttggcgtt gggcatcgta atcggttcgg tttccccgca
     7081 actgggcttg gcggcaggct tgttcggcag cctgtttgtc ggcgcgctca aagcggtcgc
     7141 gccggttttg gtatttattt tggtggcggc cacaatcgcg cagcaccaaa aaggcaacaa
     7201 ggcgcatatc aggccgatta tcgtccttta cctcatcggt acgttttccg cagccctgac
     7261 cgccgtcatc gccggtatgg ttttcccgac gcacattgtt ttggcgggcg cgggcgatgt
     7321 gtccgccgcg ccgccttccg gcattgtgga agtattgaaa tcgctgctga tgaacctggt
     7381 cgccaacccg attaacgcga ttgccaatgc caactatatc ggcattttgg cttgggcttt
     7441 ggttttgggc gcggcgttgc ggaatcacgg ttcggacgtt acgcggcagg tcgttgccga
     7501 tttggcggaa gcggtttcca ccgtcgtgaa atggattatc cgttttgccc ctttgggcat
     7561 tttcgggctg gtgtcgtcca caatcgcgga gacgggtttc ggcgcgctgg cgggttatgc
     7621 gaagctgctt gcggtactgt tgggctgtat ggcgtttatc gcgctggtgg tcaatcccgc
     7681 catcgtgtgg tggaaaatcc gccgcaaccc ttatccgctg gtgtttacct gcctgcgcga
     7741 aagcggcgtg tatgccttct ttacccgttc ttccgccgcc aatatccccg tgaatatggc
     7801 tttggcgaaa aaactggggc tgcacgaaga cacttattcg atttccatcc cgttgggggc
     7861 gaccgtcaat atgggcggcg cggcgattac gattaccgtt ttggctatgg cggcggcgta
     7921 cacccaaggc atacaggttg attttgccac cgcgctgctg ttgagcctgg tggcgacggt
     7981 cagcgcgtgc ggcgcgtccg gtgtggcggg cggttcgctg ctgctgattc cgctggcgtg
     8041 cagcctgttc ggcatcagca acgatgtcgc tatgcaggtg gtcgccgtcg gctttatcat
     8101 cggcgtgatt caggattcgg cggaaacggc gttgaattct tcaaccgatg ttttgtttac
     8161 cgccgccgcc gatttgggca gacagagaaa ccgggcggaa taaaccaagc tatgccgtct
     8221 gaagcccttt tggcgttcag acggcatatt tttacaattt tatgggcata tggcggaaac
     8281 aataccggct gccttgaata tgaaggagat gatgatgttt aaacgcagtg tgattgcaat
     8341 ggcttgtatt gttgcccttt cagcctgtgg gggcggcggt ggcggatcgc ccgatgttaa
     8401 gtcggcggac acgctgtcaa aacctgccgc ccctgttgtt actgaagatg tcggggaaga
     8461 ggtgctgccg aaagaaaaga aagatgagga ggcggtgagt ggtgcgccgc aagccgatac
     8521 gcaggacgca accgccggaa aaggcggtca agatatggcg gcagtttcgg cagaaaatac
     8581 aggcaatggc ggtgcggcaa caacggataa tcccgaaaat aaagacgagg gaccgcaaaa
     8641 tgatatgccg caaaatgccg ccgatacaga tagttcgaca ccgaatcaca cccctgcacc
     8701 gaatatgcca accagagata tgggaaacca agcaccggat gccggggaat cggcacaacc
     8761 ggcaaaccaa ccggatatgg caaatgcggc ggacggaatg cagggggacg atccgtcggc
     8821 aggggaaaat gccggcaata cggcagatca agctgcaaat caagctgaaa acaatcaagt
     8881 cggcggctct caaaatcctg cctcttcaac caatcctaac gccacgaatg gcggcagcga
     8941 ttttggaagg ataaatgtag ctaatggcat caagcttgac agcggttcgg aaaatgtaac
     9001 gttgacacat tgtaaagaca aagtatgcga tagagatttc ttagatgaag aagcaccacc
     9061 aaaatcagaa tttgaaaaat taagtgatga agaaaaaatt aataaatata aaaaagacga
     9121 gcaacgagag aattttgtcg gtttggttgc tgacagggta gaaaagaatg gaactaacaa
     9181 atatgtcatc atttataaag acaagtccgc ttcatcttca tctgcgcgat tcaggcgttc
     9241 tgcacggtcg aggcggtcgc ttccggccga gatgccgctg attcccgtca atcaggcgga
     9301 tacgctgatt gtcgatgggg aagcggtcag cctgacgggg cattccggca atattttcgc
     9361 gcccgaaggg aattaccggt atctgactta cggggcggaa aaattgtccg gcggatcgta
     9421 tgccctcagt gtgcaaggca aaccggcaaa aggcgaaatg cttgctggca cggcagtgta
     9481 caacggcgaa gtgctgcatt tccatacgga aaacggccgt tcgtacccga ccagaggcag
     9541 gtttgccgca aaagtcgatt ttggcagcaa atctgtggac ggcattatcg acagcggcga
     9601 tgatttgcat atgggtacgc aaaaattcaa agccgccatc gatggaaacg gctttaaggg
     9661 gaattggacg gaaaatggcg gcggggatgt ttccggaagg ttttacggtc cggccggcga
     9721 ggaagtggcg gggaaataca gctatcgccc gacagatgcg gaaaagggcg gattcggcgt
     9781 gtttgccggc aaaaaagagc aggattgatg ttgatgccgt ctgaagcgag ggcgttcaga
     9841 cggcattgtg atgtttgggt cgcgggtccg gttcaagtcc gcatcagttg ataaacccgt
     9901 cgtctttcat tttcttttcg gaaaaatatt gaccgcatac cagtaaggcg gcgaggatga
     9961 tgacaaacag ccccgccata gtcagtgtga ttttgaagaa caattccgct tcgagaaaag
    10021 aaaaccaaag ttgcacaatc agcagcagca cccagccggc aaacagtccg agacaggcat
    10081 aaacgccgag cttaaacacg gtcccgctcc aaaatgacga cgtaggcctc gcgtttgaaa
    10141 aacaacagca tacggcgcaa atctttttcc atcgttacaa cccgccagcc ttcgggggtt
    10201 tgtttgttga ggaacgcgct gaaattgacg gggttgacct ttgccgcgcc gaaaaacagg
    10261 ctggacaaca ggctttcctg ataaatgacg actttgtatt ctttcatagg attcctttta
    10321 acataaggga tcatcttttc agacgacccc ttaagttgct aatgatgcaa aacagcaaac
    10381 cttatttctt attgcccaac tgcggcaata ccgaaccttc gcccaatgcc aacaaaccgc
    10441 tttgcgagta aatgcccagt ttggcgcggg tgtcggtaat gtcgaggttg cgcatagtca
    10501 gttgtccgat acggtcgagc ggtgtgaacg ctgcgtcttc gactttttcc atactcaggc
    10561 gttcgggttg gtaggtcagg ttgggcgatt cggtgttcag aatcgagtag tcgttgccgc
    10621 gacgcaattc gagggttact tcgccggtaa tggctttggc aacccagcgt tgggcggttt
    10681 cgcgcaacat cagggcttgg ctgtcgaacc agcggccttg gtagagcaaa cgtccgaggc
    10741 gcaggccgtt gatgcggtat tgttcgatgg tgtcttcgtt gtggataccg gtcaccaaac
    10801 gctcgtaggc gatgtggaac aacgccattc ccggggcttc gtagatgccg cgcgatttgg
    10861 cttcgatgat gcggttttcg atttggtcgc tcatacccag accgtggcgg ccgccgatgc
    10921 ggttggcttc gaggaagagt tcgacgggat cagcgtattc tttgccgttc agtgcaaccg
    10981 gcacgccttc ttcaaagcgc acgctgactt cttcgggttt gatttcgacg ttttcgtccc
    11041 agaaggcaac gcccataata ggtttgacga ttttgatgcc cgagttcaaa aattccaaat
    11101 ctttggcttc gtgggtcgcg ccgagcatat tggaatcggt ggaataggct ttttccaccg
    11161 acattttgta gttgaaaccg ttggcaatca aaaattcgct catttcatga cggccgccga
    11221 gttcgtcgat aaattgctgg tcgagccagg gtttgtagat tttcagcgcg ggattggtga
    11281 gcaaaccgta gcggtagaaa cgctcgatgt cgttgccttt gtaggtgctg ccgtcgcccc
    11341 aaatattcac atcgtcttct ttcatggcgg aaacaagcat agtgccggtt acggcgcgac
    11401 ccagaggcgt ggtgttgaag taggcgatgc cgccggtgga aacgtggaac gcgccgcatt
    11461 ggatggcggc gatgccttcg tgtgccagct gtgtgcggca gtcaatcagg cgggcgtttt
    11521 ccgcaccgta ttccattgct tttttgggaa tggcattgta gtcgtcttcg tcgggctggc
    11581 cgaggttggc agtgtaggca taaggtagcg cgcctttgag tttcatccac aacagcgcgg
    11641 cagaggtatc aagaccgccg gagaaggcga tgccgacttt ctgaccgacg gggaggtttt
    11701 gtaaaatagt atggttttgg ctcataaggg tatccttcgg acttggaaaa aggagttcgt
    11761 cgtgtttcct tttcgattga aaccccgcca cttggacatc tgtccttcgg ggcggtagaa
    11821 tcagacttta tttgggaggg gcgtaatccc ttccaaatca ggacggcaca tagggcggtg
    11881 ctttatgtgt cgtcccgtgt gttgaaacat tatcttataa atataaccca ttgtcagccg
    11941 ttaaggctgt ctgaaaagaa gctttgcccg tttcgctgaa aatcttttca gacggtcttt
    12001 acgccatcct caacctgact ccctcccctg cgggggaggg ttggggagag ggtgcagaca
    12061 gcagccctta agcaaacgct gcctgaaaaa cgcaagctcg accctctccc taaccctctc
    12121 ccatgggaga gggaacaaat cacagcacca tcgacatttt cagacggcat cggtaaagaa
    12181 acccatctat gaacgcgttc aacctcacca tcatcggcga cgaaatctta cacggcagcc
    12241 gccaagacaa gcatttcgcc tttttcaaat ccctgctcga atccaaaggg ctgaagctca
    12301 atcaggtgca atatctgccc gacgaacccg atttgctggt caggcaactg cgccgcagct
    12361 tttcagacgg cataccgacg tttgttaccg gcggcatcgg cgccacgccc gacgaccaca
    12421 cgcgccaagc cgcagcggct gctttggatt tgcctgtcgt ccgccatcct gaagccgcca
    12481 agtttatcga aggcgtaacc cggaaacgcg gtgagccgct cgattcgccc gaacacgccc
    12541 aacgcctgaa gatggcggat tttccccaag gcgcggaatt ggtgcctaat ccgtttaata
    12601 atatcgccgg attttccatt cgcgagcatt atttcttccc tggttttcct gtgatggcgc
    12661 acccgatggc ggaatgggtt ttggatactt attacgtcgg ccgcttcaac caaaccaaac
    12721 gcggcagccg cagcgtgtat gtgtttgagc agcccgaatc gcgcattacg ccgcttatgg
    12781 agcatatcga gcaaacttat cccggtgtgc gttcgtacag cctgcccagc gtcggttgga
    12841 cgcattcaga cggcacgcag gtcaaaccgc acattgagtt cggcatcaaa gccgaaggag
    12901 aagccgtcaa ccttttggac gcggcgtggg cggaagtgtt gcacagcttg gacggattgg
    12961 gagcagagct gaaaaatcgg gtaaactgaa aaccgtttgc ccaaatcgga cgatatttaa
    13021 ggaaaaccat gcaataccga atcagaagag aaaacgaagc acccgaagca aaaaatacgg
    13081 gggaaacctt atgggagcgc gacatcatgc gcgaagtgct gctgtccgcc tatcaggaca
    13141 ggcgcaggga gcggatgtgg aaaaacatct ggcgtgccgt cagcaccctg attctggttg
    13201 ccctgattgc aggcattttc cgaaaagacg aagccgcatt gcagttggcg ggcaacacgc
    13261 cgcataccgc agtcgtcaac ctgtacggcg aaatcggcaa cggtgtagaa gaccaggtca
    13321 aaaaactcaa agacggtatg gaggccgcct acaaaaaccc gcaggcaaaa gccatcgtca
    13381 tccgcgccaa cagccccggc ggttcgcccg tcgtctccaa caccgctttt gaagaaatac
    13441 gccgtctgaa ggcgcagcac cccggcattc ccgtttatct cgtggcggaa gatatgtgcg
    13501 cgtccggctg ctactacatc gcggcggcgg cggacaaaat ctatgccgac ccgtccagca
    13561 tcgtcggcag catcggcgtt atcggcagca gtttcgacgc gaccggcctg atggaaaaaa
    13621 tcggcgtgaa acgcagggtt aaaattgcag gcagcaacaa aggcatgggc gatccgtttt
    13681 cgcccgaaac gcccgaacag tcgaaaatct gggaagaaat gctgaccggc atacacggcg
    13741 aattcatcaa agccgtcaaa accggacggg gcggccgcct caaattccga caatatcccg
    13801 acgtgttcag cggtcgcgtg tacacaggcg cggatgcgct gaaagtcggg ctggtggacg
    13861 gactcggcaa tatttacagc gtggcgcgag acgtggtcaa agcccccgat gtggtggatt
    13921 acactccgaa ggacgatttc ggcagaatct tgggcagacg cttcggcgca gaactcaaag
    13981 caagcgtgcg cgaagccctg caggccgtcc ggtagcgttg tttacagtat ttctaaggga
    14041 ataccaaaat aaaaatatac acaaaaacac tcacctcccc agtatgctgc cactaagtca
    14101 gccgcgtcac tatttaccgt gcacttaaag ccgtaaaagg caggttgtcc ccgggaggtt
    14161 ttgtgcatcc tgataccgat cggttgttgt tgctcaaagg acagaaggcc gctgataaac
    14221 gagattacct gtttgtcgct attgacgatt tttatactct gccattttgc cagacaaaac
    14281 cgcagacagt gctgccaagt ttctgaccga acatctggcc gacccctgct tgtacctgat
    14341 tgagtacgtt tactctgaca atgataggta atataaagag ccgtccaaca tgctttcggt
    14401 gcagcttgtt ataataatgg gattggttgg aggcttgccc gatttgcttg tccgcagacc
    14461 aacggtaagg cggagcgggt tatccgtacc ttgatggaga tgtggcatga ggaacagtcg
    14521 tttgacagtc cggaacatcg gcaaaaggag ttgtgctgct ttattaattt aggggctacc
    14581 ctagatcact agggaaattc aaattaagtt agaattatcc ctatgagaga aagtcgtcta
    14641 agccggtata aacaaaataa actcattttg ttatcgtggc tatgatgtat tggatgcggg
    14701 cgaatttagc catttccgta tcaatcacag cacacatttt gccgaacgac aaaaccatat
    14761 taatggaact gagaactttt ggaatcaagc aaaacgtcat ttacgcaagt ttaacggcat
    14821 tcccaaagcg cattttgagc tgtatttaaa ggagtgcgaa cgacgtttta acaacagtga
    14881 gataaaagtt cttgttccat tttaaaacaa ttagtaaaat caagtttgtc ctagttgtcc
    14941 agggcagcct catctcttta tagatagctg cttaccaagt atccttaatt gctaattcaa
    15001 gccaatcttc aaaactatta aaaaattcat ggttttctat attatctaaa ttaatgacaa
    15061 taaccctaga atttcctgtt acatcattac catcaaaata agcttcccat tctccattac
    15121 tagcgaatgg gataagattt aaatcaggca ggatagaatt tctaaatata taagctactt
    15181 ctgccccttc tgttccataa ttctcaaacc accagtgaaa attatgtggc tgaatataag
    15241 gtattaaaga tgtatcgtta gagatacgaa catacttttc aggataacaa aacctttcag
    15301 gaagcatatt actaggatat aaattaaatt cattttaaat aatcatttcg ttaccttagg
    15361 atcataaata ctaccattag gaaactgggt agaccaattt ggtggtgcta gttcattttc
    15421 aatttgtttt ttaattctaa attgaatagc ttctccccat gtttgcggca tctaatgctg
    15481 cttgctctgc atcaatatta ctaaaaattg gattagtctc aaaaatgaaa aattactgtt
    15541 ttttcatttt ttatagcata ttcaagtaca tgaataatat ttttcactaa gggctctgta
    15601 gaaaaatctg aattttgtaa tttttctttt acagccaaaa tactttcctt atctattagc
    15661 caatccccct cacttggtcc aaataaccaa ttatcggtaa tttcagaaac aagtctccat
    15721 aatccacgat tttgatattc aataatttga ctttccgtaa tttctacaac tgtttcagat
    15781 tgaatgtttt tataagccca atcaatcatt tcagtatatt cactaattga aacattgtct
    15841 tttaaacaga aaattaacag gttgtgttca ttcatttgtc taagttctta atatgagttt
    15901 gttgttgtaa ataatctttt gcttgcttac cttgtaaatt aaaggattaa ccgcaccttg
    15961 gatgatgccg cgaatatcat ccatacggtt accccaccaa attttattgg tgtatcgtta
    16021 atcatttatt tcaaaaaagt tatttattat catattatca gggagcaggt cgttaaaaaa
    16081 aaccccctca aattccatta aattagtatt tatccagcac ataaacgaac caaagcgttc
    16141 tttccctgag tgtaaatatt taaaattaaa ctcaaaataa gtttttttgc ttagaaaatt
    16201 taatggtttt tctatagtga aaaaaaagcc cttactcgtt ttagttcgag atactatttt
    16261 aaataaattt ttattttcaa taaaataaga atattcattt actaagttgt tatgataaaa
    16321 aagtaatttt attaaaatgt tttcataatt ttctaggttc atttctagct caatttaaaa
    16381 tgatttcatt gttgccgtac caatgcgaaa tttaccatca acaaaatttc ccttcactga
    16441 tacaggtacg ccatcaactt gggtagtttt attttcaggc tgcctgaaag aaagataggc
    16501 aacttattac tcatcttacg ctagccctca ataattgaat acgttgcact agaaccaatt
    16561 atttctatga atgtttgctc aaacataaaa atatagtatt taaagaattc tctattcaaa
    16621 ggaaaataac tttctttcaa tatccattcc gcataacact catcaagaag ttcaaaaaag
    16681 attgaccacc attttcatga agcaaagctc tatctgcatc ttccgaaatt aaatccataa
    16741 aagtatctat aaaatacgat tcatcaacaa ccctaaaatg aataacatct tcaataaaaa
    16801 tttttatggt tttatccgta tttctataac caactgctat aacttctact ttatcagagt
    16861 ttattgctgc attatcaaaa tagaaatagc cagttgggaa atctaagctt tttaatttca
    16921 taatttatcg tgtatttcca tatctgattt ttatttttcg gttattacta attgttattt
    16981 caagtgttgg ttctccacca ctactatcat tacgagcatt aaccgcacgc ctatcaggta
    17041 aattgcccgt tatcgttcca ttaggacgtg tttgtacatt tcgaacattc agactattaa
    17101 aatcatttag tgcgtcattg taatccccca ctttatcata ctgaacagcg ggaccttttg
    17161 ttttactcct ttgagtagta ttgctcaaaa tgtctttgat ttgtaaatca ctgcgcacct
    17221 taccggtttc tatgcgagct gccacactac tcgggctata ggggtcgcta tcaatcgcag
    17281 gattaccacc gctggttttt tgtccgctag cctgtttccc aacctgctgt actgtatcgc
    17341 caatttcatc taccgtacct aacgccttag tctgcatatg gcgaacagca ggttttttat
    17401 agccggtatt tttaacccaa tcccatttag tcgggttaag ttctactttt ttacctctcc
    17461 aaaccgtacc tgcggcctct gctacggaaa gggcagtttg ggctgttgca gttatattcg
    17521 gatgggcatc agcccattgt ctggcagagt tgatactgtc ttttaccgca aaagcactgt
    17581 cttgtaatgc ggttgcggcc gcaagttgtg cttcgggact taaatttcct aaattattaa
    17641 taccttgtag agtctgctgc gcagagatgt agcaactgta tttttacccc gtcgggtaaa
    17701 aatacagttg ctacggctgc tgggaaagca gttactattt tcccagtggc tcaaaattaa
    17761 ttgtttcagc agatattttt ataataatcc ctacatcaat aataatttca ctataaaatt
    17821 ggtatttata tagctctatt tcgggtataa acaagggaaa caagctatct tccttatcct
    17881 cataatctac gaaattattt gtatctaatt taaaattcaa aatattactg catttgatat
    17941 atccgaaata attaccttca attgtatctg taaaagataa tgtaagggta tttccattat
    18001 cggaaaagat tatttcataa aggcttacat tgtgaaaaat attttttttc atttgacaaa
    18061 atatccagaa tgatgacgat tatttggcaa tggattgcct tcataatctt gtcgttgcgg
    18121 atattttccc tgtacaccat cggttcgcgg cactctttgg gttcttataa cctctccatt
    18181 tggacgaata tctattttca cgccatcagg acctttccat gtttcatatt gagcctcatc
    18241 tgaaccagta cgtctaaaac cttgttcttg caatgcttga cgtaattcat ctcgcgtagt
    18301 tggtctgata gatttaacaa ctggcttgtt tcctcctgcc atctcaccgt ccaacgtctg
    18361 cataggtcgg gcagcaggtt ttttatagcc ggtattttta acccaatccc atttggtcgg
    18421 gttaaggttt acttttttac cgccccaaac cgtacctgcg gcctctgcta tggcaagagc
    18481 agtttgggct gttgcagtta tattcggatg ggcatcagcc cattgtctgg cggaattgat
    18541 gctgtctttt accgcaaaag cactgtcctg taataggctc gcggcagcaa gttgtgcttc
    18601 tggacttaat tttcctaaat cattaatacc ttgtagagtc tgctgagcgg ctgtatctga
    18661 gccggtcagt gtaggttgga ttaaaaccca gcattttcaa catcttgatg ttttactata
    18721 aatctattct tgtgcttcct ttgttagata aatttcaaca caaaagatac gtatgcattc
    18781 tctaatttta tttgagataa ataatgcatt gctttgtcga tactgttaaa taaattactt
    18841 ccctcgtaat accaattatc atatgtttga taaaaataat tatctttctt gatataaata
    18901 tctcccccca atacaagtat tctctcattg ataaataaat ttaatatatc tattgcaata
    18961 gggacgggta atgcggcttc tctaattcca attcctatca aagattcccc ttgtctcgaa
    19021 taaatttcaa aaatttcatt taacatgtta atctctatct actatttttc aatcggtata
    19081 aaccctattc acattttatt tttcctaaaa ccaaatcttc gactaactgg gttgcataaa
    19141 taatatcatt tatgatttta acaggtaaat tatttgatag gtaaccatgg gattccgtca
    19201 tcattgattt ctctaggtgc ttatttatct gattggtaag ccttttgcgt aagcttcttt
    19261 cgcctccgca agtgtcattt cattaggtcc accatctata ttagtttctc ctgtattttg
    19321 ccactgacaa acgtcacaga tatcatagtc cataacttca gttccgcaaa caggacaatg
    19381 tagccatatt tctccgtcaa tttcccatgt cttttttgaa ttctccatca taatacttct
    19441 ttcctaagtt tggtttaaac attgttttga ttttgaatgt ttttggattg gccaaaacat
    19501 agatattgtt attgatatca tagcgcactc gtctatgttc cgtctgataa cctaatatct
    19561 tgtccgatgt ctgttttgat aacaaatcag acgccatttt ttggtagtct tcaatggtta
    19621 tatcgccaaa ttcttttcca tgagctttga aatgtccatt taaagatttc tcagtaggaa
    19681 acttggactt tgtccatctg atgcggtctt gaattgttcc tgtcggagaa atagatgtat
    19741 caggtttaac atatgaggca ggtaagccga tattgttatc agagtgtgta gggtgttgct
    19801 gcgtacttgg ttttggtggc ttattcccac ctgccatctc cccatctaaa gtctgcatag
    19861 ggcgggcagt aggtgtttta tagccggtat ttttaaccca atcccatttg gtcgggttaa
    19921 gttctacttt tttaccgccc caaaccgtac ctgcggcctc tgctatggca agggcggttt
    19981 gggcagtagc tgtaatgttc ggatgggcat cagcccattg tctggcggaa ttgataccgt
    20041 cttttaccgc aaaagtactg tcctataatg cgcttgcggc agcaagttgt gctttcggac
    20101 ttaaatttcc taaatcatta ataccttgta aagtttgctg cgcagagatg taggtcggat
    20161 atttgtatcc gacaaaaata tttgacgcat ctatcgtttc cgaaatgccg ctgttggaaa
    20221 atgtcggatt cgagaatccg acctacggct atttcgttta agtcataaat agccattcat
    20281 gttccttctg aatttgcttt tgctatcctt agaaccaatc atataaacat tatcatcatt
    20341 aattaatgtt ctaagtttat gaagcggtca aatctgtcaa actaccagag atgaagcata
    20401 tcctcctgga agtaattctg ttcgtgctgt ttccatatct tctttgccat catcagttac
    20461 ttctctgtct aaataaaatc ttacgataag tttttgtcta atttcatcaa attctgccgt
    20521 aattaatctt atattgatat gaatacacgg ttcatacctg ctattagcca gtcaggtgta
    20581 gttggatggg gattcatttt taataatatc ttcaagcaag tgaaatgtaa gaaaagcgta
    20641 gatacttgaa ggccggctat ctgattggca aaaagaaatt tcggagataa agaatttccg
    20701 tttatatttt tcataaatta aatcaacacc ttgttttaca taaaaactaa cttcctcagg
    20761 aaggatattt ataacattct ctccttttac ttttatagtt aatggtttga tttttatgtt
    20821 ttcatgaata tctgatagga ttatttctaa aaaattattt ttatttccag tcattctggc
    20881 tattttatgt atatttccat tgtgttcgat tatcatttta ttttttccaa ggatttcctt
    20941 ctatattgat agtttatcta aagatttcat ttaaaatatc ctgactaatt tcttgtttat
    21001 ataattctac aattttctgt ttctgtgtat ttgtataaaa tttttcgttg gtatctgctt
    21061 gatattttaa ttgtctcaat atttccatga tttcatattc tgatttttca tcatattctt
    21121 caatcatgta atcaacaaat tgttttagaa tatcaaaggc ttcgtaactt tccaaaattt
    21181 taatatgttt atcggcaaaa ttggacatat accattcgtc agtgattgga ttagtattta
    21241 aatcatctaa aaaaacattt aaatcatcta aaaaaacatt tacatcatct aaaaacatga
    21301 ctaatcctta aacttaaaat ctgttttctc accggttctt gagttttcaa tgtaatgtat
    21361 ttcaattttg tttacgtttt gaacacgctt aacccaaccg tcttttgcta accaaccatt
    21421 tttagtatcg gacataggag gtattctcgc aggagttttg ccctgtgggt ttgccttaac
    21481 ttcttccatt gctaattgtt cctttaaatt attaggtatt gtacgaccgg ttgatttttc
    21541 aacagattgt gtccctattt ttaatgattt gtttccccca gccatttccc catctaaagt
    21601 ctgcataggg cgggcagcag gttttttata accggtattt ttaacccaat cccatttggt
    21661 cgggttaagg tttacttttt taccgcccca aaccgtacct gcggcctctg ctatggcaag
    21721 agcagtttgg gctgttgcag ttatattcgg atgggcatca gcccattgtc tggcggaatt
    21781 aatgccgtct tttaccgcaa aagcactgtc ctgtaatagg ctcgcggcag caagttgtgc
    21841 ttccggactt aaatttccta attcattaat atcttgtaaa gtttgctgcg cagagatgta
    21901 ggtcggatat ttgtatccga caaaaatatt tgacgcatct atcgtttccg aaatgccgct
    21961 gttggaaaat gtcggattcg agaatccgac ctacggctgc taccaaccat cattttcagc
    22021 ttcttttagc caagtatcaa aattttcaaa agtctcgtaa tattttgtat tatctaaatt
    22081 aactactata attttaggat tacctgattt atctgaaata tcaaaacagg cagcccactc
    22141 ttggtttcta gcaaaaggta ataggtttgg aatgccagtt atttcaaaat aaatgtcaat
    22201 tacttctgat atattgcttt ctgcattttc aaaccaccaa ggaaaagaat attgttcatc
    22261 gtagttaatg gcatgagtgg attgagctaa ttttaaataa ctttgcggat atttaaatcc
    22321 tttaggaagc attgcttcag gatacggaac aaaactattt tgttttatca tttcctaagt
    22381 acctttacat cataaatact tccgttagga aatcttttgc tccacccttc tggaactcct
    22441 ttttgtgttt cttgctttct aattcgtagc tggatagctt ctccccaagt ttgaggctca
    22501 ataccatcat gcttaaacct gattctctct tttgatgata atcggtcatt aaaattatga
    22561 acattcgctt ccacttttac tcctgcttca cgtgctgcca taatacgcgt gttatccata
    22621 ctagtaggtg ctccatcagg catattaaca acatcaaccc tgtcgccaac ccatgatttt
    22681 tttctttcat ttcggctaca atagaatcat agttgtaatt tgcactaggc tctgacaaaa
    22741 atttgggttg ttaagttgtc atttttttat aaattccagc aaactgttgt tgtaaattat
    22801 ctacttcatg ttttctaaca ccagatacat tttcaaattc tcttgaatct acacatacgc
    22861 ccgcatatat ctcattcaag atagctcgta aaacaaaaac ttcatcttga gttagtgaaa
    22921 tagttgagtt ttgattattt atggatagta tattcatggc tttttcctat aaatcatcat
    22981 aatatttttt acctgatgtt ggtctaaatg ctgtacctcc atcgtcagaa tttttatctc
    23041 ggataactat tgtccctgtt ttatcatccc aatacgcagt tctaccgcga ggtaactctt
    23101 tcatatttgt tggatggcta acaatatttt caatatgccg agcaaaatct gctggtgaat
    23161 tgatatttaa atccgtaaat tcttgttgtc ttatgacatg cttgttataa gcatgcccac
    23221 tactaatttg ttctccaatt agttgcgctt gtaaagacgg ttgtgtggaa gcatctgctt
    23281 tgctgttgga cgttatagat ttaggcggtc tattcccacc ggccatttcc ccatccaaag
    23341 tatgcatggt gcgaacagca ggtgttttat agccggtatt tttaacccaa tcccatttgg
    23401 tcgggttaag ttctactttt ttaccgcccc aaaccgtagt tgcggcctct gctacggcaa
    23461 gggcagtttg ggctgttgct gttatattcg gatgggcatc agcccattgt ctggcggaat
    23521 taatgccgtc ttttaccgca aaggcactgt cctgtaatag gctcgcggca gcaagttgtg
    23581 cttccggact taattttcct aaatcattaa taccttgtag agtctgctga gcggctgtat
    23641 ctgtgacagg gcttaccgca ctgtctgtaa ttgccccaat ccctactcct tgaaaaccca
    23701 ttaaaaaagg attaaccgca ccttggacga tgccgcgaat atcatccata cggttagccc
    23761 accaattttt attggggtcg ttttcccata tcagttttcc tgcttcatca gcacagttag
    23821 gattggcatt gatgcccacc cagtttcagg cagcctgaaa aggattttta atatacggca
    23881 aaaggtgggc aacaagtgcc caccttacct agctaacatt tatcccaagt atcatatatt
    23941 tttttaagtt ctttatccaa agaaagaact ttattttttc aacaccatga attatctcaa
    24001 aatctctaca atccacgcat acgcctgaat aaatttctcc tacaattgct tgaataatat
    24061 acaactcatc tttatcgaga ctaattttaa ctgaactctc ttcaaaatta attaatttca
    24121 tgaataattc cttttatttt aattcgtcat caaaataccg tttgcctgtt tttggtctaa
    24181 atgctgtcca ccatctttag tcttggatat ttagattata atcgtcaaat tcccagtcaa
    24241 aatattttgc caaatttgat atagacttat cttcagacgt tgaattacat atttccaata
    24301 tatcatttat caattttaat atgtgttcaa tattaaaacg gtttgtaaca attgagtttt
    24361 ttaatgacat cggtctatgt atatttgaaa gtaaccattc agtgctatat atataaacat
    24421 aaaaataatc agcaccatta ctttcatcaa aacctattga taatgtcaaa agtatacaaa
    24481 aattttctaa cttgtcttgt gggaaatctt ccaaatcaaa gtcagaacaa tcataatttt
    24541 ttaattttaa tctcatataa ttacctaata tttaaatgtc catttttaat tttattcctt
    24601 tttccatttg aatcaatagt ataagtttca aaatttgctt gaatacctgt agttgcaaat
    24661 tgtgaatttt tttctgttgg tggccgatat tgtcgagtgc catctatact taaccatccg
    24721 cctccactag tttgtcttgc accctcacca acccaaattt tacctagcct atctgcctct
    24781 tcataagttg cagttcctat tggaaaattt ttttcactct tatgaagagc tagactcaat
    24841 cttggatctt gagccgcaat gttttttaag ttttgcccag ttaattgatt aactaattga
    24901 ggataagttg cagcattggc ttttcctcca gacgttatag atttaggcgg tttattccca
    24961 ccggccattt ccccatctaa agtctgcata ggtcgggcag caggtgtttt ataatcggta
    25021 tttttaaccc aatcccattt agtcgggtta agttctactt ttttaccgcc ccaaaccgta
    25081 cctgcggcct ctgctatggc aagggcagtt tgggctgttg cagttatatt cggatgggca
    25141 tcagcccatt gtctggcaga gttgatactg tcttttaccg caaaagcact gtcctgtaat
    25201 aggctcacgg cggcaagttg tgcttccgga cttaaatttc ctaaatcatt aataccttgt
    25261 agagtctgct gcgcagagat gtaggtcgga tatttgtatc cgacaaaaat atttgacgca
    25321 tctatcgttt ccgaaatgcc gctgttggaa aatgtcggat tcgagaatct gacctacggc
    25381 tgctgactat tttcttgttt atataattct acaactgcaa actactatca aatcttttta
    25441 tctctggctc tacactcagc aagaatattt ttattttaca aaacataggc tagttatttg
    25501 agtatattgt caaattctaa tttgtttttt tgatagattt tttttatacc attaatacat
    25561 tcattagttg taattctacc aagcgaatgt aatttatcaa tatcaaaaca atcggtataa
    25621 accctattca cagttttatc ttgtatttcc ttatgaatac ttaatatatt gagatagttt
    25681 agaaatttta ttttctcaga ttgaggtaac tcatcaaaac caagccaata ataaaaattg
    25741 ttatatgatt ttaaaaaatc aacaattttt atttttccta aaaccaaatc ttcaactaac
    25801 tgggttgcat aaataatatc atttatgatt ttaacaggta aattatttga tgggtaacca
    25861 tggggttccg tcatcatttg taactcctgg tacatgtgcg tgtcctgtaa gtgcgcgtgg
    25921 gtcttgatgg gtatttgcat gatgagcatc atctattctg gctccagtca agtttccatt
    25981 actatctcgg acttggataa aacgagcatc aggcgaacct gttatataac cgcctattgg
    26041 tgttggtatt gcatttcctt tgctgtctac ataaattgtt cctgtcggag aaatagatgt
    26101 atcaggttta acatatgggg caggtaagcc tatattgtta tcagagtgtg tagagtgttg
    26161 ctgcgtactt ggtttcggtg gcttatttcc cccagccatc tccccgtcta cagtctgcat
    26221 aaggcgaaca gcaggttttt tatagccgtt atttttaacc caatcccatt tggtcgggtt
    26281 aagttctact tttttacctc tccaaaccgt acctgcagcc tctgctacgg aaagggcagt
    26341 ttgggcagta gctgtaatgt tcggatgggc atcagcccat tgtctggcag agttgatact
    26401 gtcttttacc gcaaaagcac tgtcttgtaa tgcggttgcg gccgcaagtt gtgcttccgg
    26461 acttaaattt cctaaattgt gaataccctg taaagttttc cgtgctgccg cataggttac
    26521 cgggtttacc gcactgtctg taattgcccc aactcccaat ccctgaaaac ccattaaaaa
    26581 aggattaacc gcaccttgga cgatgccgcg aatatcatcc atacggttag cccaccaatt
    26641 tttattgggg tcgctttccc atatcagttt tcctgcttca tccgcacggc tgaaaaaacc
    26701 agaggcggca ccggcatttt cttttagcca acggtctgaa aatggggctt gagggacaat
    26761 attagtcttt gtttttgttg aagttccttt gacataatag ctgtatatat cccttgctcc
    26821 tccggggggc ggataatcgc tgccttgcgg cccgtcatat ccatcctccg gatgaatttc
    26881 cgaccctgtc cgatgaagtt ggtaaacagt aaaaccgccg tctacaccgc cgctgaaatc
    26941 agaagtgctt tttgaatcat gattatcgaa cggactgtgt acttcatgtc catgtcctga
    27001 aaaatgggtt tcataaccga taatgccttc aaaccctgtc cgttcgtgtg taataggcag
    27061 tacaggactg accgcagttg catcaaatgt ctggacggcg taaacccgat ttttaacact
    27121 gccgcgagca ttaccaaaca gatggtattt accacccggt tcgtagtgtt ttgcctgcat
    27181 atcatcgcgt aaacgggcat ccaaaccgtt cgcatgacta atggggattt gcagcaacga
    27241 tattgccgct gcaaacagca tcatgaattt ttgaataggc aatttcattt tgttttcctt
    27301 ggtagcaagc ggttatggtc gtgtgaatca aggttgccct tgtctatgtt gtcgcactac
    27361 ttcatcgctg tatccatacc cctcatgact gttatcagtc tctacggatg gggcggagtt
    27421 acccgtatga ttgccgtatg gtcggatatc ggagaaatcg accattaatc cttccgtcgg
    27481 tttaattcct ttgcttactt tatacggccc catccacaat gcgtaatttt ctttataggc
    27541 agcttcaaac gcattggttt tgggtttgat gagcaatttt ttattggttc tgtctactgc
    27601 gaaatattcc agttttgttt gggctttcag tgtttcggca ttgtataggt gcatttcggt
    27661 tctgttgcgt atcgttccga atacgtcgat gttaataaac acatctgtat cggcattggc
    27721 aggagaaaca acgtctatgc cgcgcaggaa aaatacggtc tgtaccaagt gggaaagaaa
    27781 ggcagtgtcg cgcgggttag tcgtcaaggt ttcatttcga taatccccca tcccgccaat
    27841 atttaagccc agactgctcc tacttccgct accgtctgat tgggtgcgcg agcgtgcagg
    27901 ggcattaagt gtagataaag aagtggttaa acctgtcaaa ccgcctgatg ttgtttcagc
    27961 ggtggtttcg taacgtggat aggtgtaatc ggtacggacg gcagggctgt ttatgtattc
    28021 gccacgaatc agtgcatcaa tggagtagcg accccctgtc aaactgcctg aaccttggtc
    28081 gcccatagtt gcaatgtaca atgcaacttt tcgtccgtgt aatgcctgta aatccatgtc
    28141 tttaacggca gctctggcag aagcggccac aagttcttgt tcgaccgcaa agcgtttacc
    28201 tccgccatgc gatggaatac ctgtcagtgt cccgcaggcg gataaaataa aaactgaaaa
    28261 aagaataggt atcagcagcc gtgcttgcat agattttctc ctttgatgaa aaacaaattg
    28321 tatcaaaatt gtaaatatag tggattaaat ttaaaccagt acggcaaggc gaggcaacgc
    28381 cgtactggtt taaatttaat ccactataat ctccatataa taaatggctg tctgaagccc
    28441 atatgcgact atatctgccg acactatggt caatcagcag tgaaccagcg taccttcgtt
    28501 ttcaccggtt actacacgtt tgagcgagcc ttctttagca atgccgaata ctacaatatt
    28561 gagcttgcgt tcgcggcaga gggcgaaagc ggtggcatcc atcactttga ggtttttcaa
    28621 caaggcttcg tcaaaagtaa tggtttcgta gcgcgtggcg gatgagtctt ttttggggtc
    28681 tgcggtgtac acgccgtcaa cgttggtggc tttgagcatc acgtcgcagt tcatttccgc
    28741 accgcgcaat gcggcggcag tgtcggtcgt gaagaacggg ttaccggtac cggcggcaaa
    28801 aatcacgact ttgccttctt ccaaatattg gatggcttta ggacgggcgt aagtttcagc
    28861 gatttgctgc atagacagtg cggattgtac gcgcgctttg atgcctaaag tttcaaatgc
    28921 gtctttgagt gccaacgcgt tcatcacggt cgccatcatg cccatgtagt cggcggtggc
    28981 gcgatccatg ctgcctgctt gggcggatac gccccggaaa atattgccgc cgccgacaac
    29041 aataccgact tgcacgccca ttttaacgac ttcggcaatt tcgccgacag tttgaacgat
    29101 ggtatcgtga ttgatgccga acggatcgga acccatcagg gattcgccgg agagtttcag
    29161 taatacgcgt ttgtatttga tttgctgtgt catgggatac cttgctttct tgaatgtcgt
    29221 cggtcggagt ttgggccgtc tgaattttca aacggtcgga attacaatga tatggtgtgt
    29281 cgttctgtta tagtggatta aatttaaatc aggacaaggc gacgaagccg cagacagtac
    29341 aaatagtacg gcaaggcgag gcaacgctgt actggtttaa atttaatcca ctatattgtt
    29401 ttgttttcag acggccttta tattcggtcg tcttaaaata ggtaccgcaa acggttttct
    29461 caaaaaaaag caccctgatt cgtttggaat ccaggtgctt tctttttcat aagtgcctta
    29521 cactttggcg gctgcggcta cttcggctgc gtagtcgaca acggctttct cgataccgtc
    29581 gcctactttg tagcggacga agctgaccac ttcagtgccg ttttctttgg cgaattgggc
    29641 aacagtttga tcggggttca tcacgaatgc ttggccgttc agagtgattt cagccaagaa
    29701 tttgcggata cggccttcaa ccattttagc ggcgatgtcg gcaggtttgc cggaagcgat
    29761 ggcttgctcg gtgtagatgt ggcgttcttt ttcaacggtt tcggcatcta cttcggcttc
    29821 gcttacgcat tgtggtttag cggcaacaat atgcataccg attttgcgtg ctacgtcttc
    29881 agagcctttg tactcaacca atacgccttc ggtcgccaat gcgccgtgga tgtaggcaac
    29941 cagttggttg gcagtgtcga tcacttggaa gcgacggaca gacatattct cgcccaattt
    30001 ggcgatgatg gctttgcgtt ctgcttcaac cagttcgctc agttcttcaa cagaagccgg
    30061 ttttttctcg gcagcagttt tcgcaacgaa gttggcaaat tctacgaagc ccgcgtcttt
    30121 agcaacgaag tcggtttcgc agtttacttc gaccaatgcg ccgacattgc cgttgatcgc
    30181 gtaagccaat acgccttcgg cagcggtacg gccggccagt ttaccggctt tcgcaccgga
    30241 tttgatacgc aggatttctt cggctttgtc gaagttgcct tcggcttcaa ccaaggcttt
    30301 tttgcattcc atcatgccca ggccggtagc ggcgcgcagg tcggcaacca tttttgcagt
    30361 aatttctgcc attttgaatc tcctagattt ttgggaaata cggaaaggat tttccgcatt
    30421 cgggaatcgt gtgccgcgaa cggacgttca gacggcatat ttgagaaaag gggcataacg
    30481 cccctcttcg gtttgccgga ttactcggca gcggcttctt gggcagcggc tacggtttct
    30541 tgcagcgctt ggtttttgcc ttccaaaact gcgtcagcga tgccgcggca gtacaggcgg
    30601 atggctttgg cggagtcgtc gttgccgggg atgacgtatt tcacgccgtc agggctgttg
    30661 ttggtatcga ctacggcgat aacggggatg cccagttttt cagcctcgac cagagtacct
    30721 ttttggtagc cggtatcgat aacgaaaatc gcgtcgggca ggcctttcat gtttttgata
    30781 ccgcccaaag aacgttccag tttttcaaca tcgcgttgca tttccagaat ttcttttttg
    30841 ctgaaaccgc tttcggcagc attttccaag gctgcggttt tttcttccag gcgtttgatg
    30901 gattgcttaa cggttttgta gttggtcagc ataccgccca accaacggtg atcgacgaaa
    30961 ggcatgccgg cgcgggtcgc ttcttcgcgg atgatgtcgc gggcttggcg tttggtacct
    31021 acgaacaata ctgtaccttt gttggcaacc agacgacgta cggcttcttg cgcgtcttgg
    31081 aacatcggca gggttttttc caggttgacg atatggattt tgttgcgcgc accgaaaatg
    31141 tattgagcca ttttcgggtt ccagaaacgg gtttggtggc cgaagtggac accggcttca
    31201 atcatctgac gcatagtaat ttgagacatt ttgatttcct tgaaagggtt aagggcacac
    31261 attcaatcgc atagaacttg atagaccgcg cctattgtca agcacccttc ggcgaaagtg
    31321 ggcataagtt ttaaaaaata aaagagtgtt tccgaaatgg aaaactgacg gattatagcg
    31381 gataagaggt cgtctgaaaa gggattcagg gttttgtgcc gctgtctttt taccgttgtg
    31441 cacgctgctg ttcgaattct gcctgttcct gttgcttcat caggcggttg cggcggcgga
    31501 tggtcacgac aaagaccgtg aacacggtgg gcagcaccgc ccaaaaaacc aaataaatca
    31561 aggcgcgcgc aatactcggc tgcgcggcgg aaaacatcac ggcaacaaaa agatagccga
    31621 tggcgacaat gtgccacatt gtgtgcgtcc tgtttgaatg ttaacaaaat cgttataatc
    31681 gcaacattct accgcaatcc tgaaaggccg tctgaaatga ggaacgagga aaaacacgcc
    31741 ctgcgccgcg aattacgcgg gcggcgttcg caaatggggt gggacgtgcg ggcggcggca
    31801 acggtaaaaa ttaaccgcct gctcaaacgt tatatcaagc acggtcggaa aatcggcgtg
    31861 tattggccga tgggcaagga attgcgtttg gacggctttg tccgcgcggc gcaaaaacgc
    31921 ggcgcaaaac tttatctgcc ttatatcgaa ccgcgttcgc ggcggatgtg gtttacgccg
    31981 tatcctgaaa gcggaatgga acgggagcgc atacggggca gggcgaggtt gaacgtgccg
    32041 cagtttgcag ggcgcaaaat ccgcgtgcac ggtttgtcgg tattgctcgt tccgcttgtc
    32101 ggcatagacc gcgaaggcta ccgtttgggg caggcaggcg gctattacga tgcgacgctt
    32161 gcagcgatga aataccgttt gcaggcaaaa accgtgggcg tgggctttgc ctgccagttg
    32221 gtggacagac tgtcggtcga ggcgcacgac ctgccgctgg acggttttgt gtcggaggcg
    32281 gggatattgt gcttttagcc cttcagacgg catttcgggc ggaaatgccg tctgaaggcg
    32341 gaatattggg agaaaaggat gtcggaaagg ataatactgc cggtcttgtc attgggcggc
    32401 gttcggctcg aaccgttgga tgtgcatcat gaaacgggtt tgcgcgaggc ggtttgcgat
    32461 ggggaggttt ggaagctggg ggtaacgtcc gcgccccatc cggatagggt ggcagactat
    32521 atcgggacag cattggcaac gcgtttggcg tttgcggttg tcgatgaaga ggcgggcagg
    32581 gtggtcggga caacggcgta ttatcatttt gaaccgcaga ttccgcgttt ggatatcgga
    32641 ttcacatggt atgcggcatc ggcacggcgg acacggataa acacctgttg caaaatcatg
    32701 ctgttggatt acgcatttga tgttttggct tgccgttgcg tgggatggcg cacggatatt
    32761 ctgaaccttg cctcgcaacg tgccatcgaa cggctgggtg cggaaaaaga cggcgtgctg
    32821 cgtatgcata tgctccgtaa agacggcagt gtgcgcgata cggtcgtgta cagtatgctg
    32881 agggaagatt ggtgcaaaaa cagggaaatc ctgaccggca ggttggcagg gtatggcgtg
    32941 caggtgtgag gcagtatttt caaat
//
LOCUS       DAWFLT010000016        32737 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWFLT010000016 DAWFLT010000000
VERSION     DAWFLT010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32737
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.016"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..332)
                     /locus_tag="WGR74_000734"
     CDS             complement(<1..332)
                     /locus_tag="WGR74_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0905194.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGVNKSTAAYYFHR
                     LRLLIYQNSPHLEMFDGEVEADESYFAERQNHINGIENFWNRAKRHLRKFNGIPKAHF
                     ELYLKGYER"
     gene            complement(638..1897)
                     /gene="rho"
                     /locus_tag="WGR74_000735"
     CDS             complement(638..1897)
                     /gene="rho"
                     /locus_tag="WGR74_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ0905195.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            complement(2117..4501)
                     /gene="ppsA"
                     /locus_tag="WGR74_000736"
     CDS             complement(2117..4501)
                     /gene="ppsA"
                     /locus_tag="WGR74_000736"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ0905196.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTAEAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFAINGDKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLENGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4912..5733
                     /locus_tag="WGR74_000737"
     CDS             4912..5733
                     /locus_tag="WGR74_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ0905197.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(5973..6635)
                     /locus_tag="WGR74_000738"
     CDS             complement(5973..6635)
                     /locus_tag="WGR74_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0905198.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLIKLGAGITPDHPDYARWRTEYLEEYDRRYAQDTELFDGVNELIAELNQRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(6648..7475)
                     /locus_tag="WGR74_000739"
     CDS             complement(6648..7475)
                     /locus_tag="WGR74_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ0905199.1"
                     /translation="MKPDIYALLELALLSDDPDEKGQLTDEAFAAVQNIDGAEANAPP
                     LDFRHAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPVALFRSLITRYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKNTV"
     gene            7752..8375
                     /gene="lolA"
                     /locus_tag="WGR74_000740"
     CDS             7752..8375
                     /gene="lolA"
                     /locus_tag="WGR74_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ0905200.1"
                     /translation="MMKPHKLFQFLAVCSLTAAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTKPYRQTIVGDGQTVWLYDVDLAQVTKT
                     AQDRAIGGSPAAILSDKAALEAGYALEEDGSSGGIDYVLATPKKNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGSLNTKPGLSRGAFKFTPPKGVDVLSN"
     gene            8648..9787
                     /locus_tag="WGR74_000741"
     CDS             8648..9787
                     /locus_tag="WGR74_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0905201.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQAPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            9919..10975
                     /locus_tag="WGR74_000742"
                     /pseudo
     CDS             9919..10975
                     /locus_tag="WGR74_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            11016..11546
                     /locus_tag="WGR74_000743"
     CDS             11016..11546
                     /locus_tag="WGR74_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ0905202.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDEVMIGAGSLVPPRKRLAGGYLYIGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(11557..11696)
                     /locus_tag="WGR74_000744"
                     /pseudo
     CDS             complement(11557..11696)
                     /locus_tag="WGR74_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor"
     gene            complement(11715..13310)
                     /locus_tag="WGR74_000745"
     CDS             complement(11715..13310)
                     /locus_tag="WGR74_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ0905203.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIENPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATVRMVEPDEAKFSGFIFKIQANMDPKHRDRIAFLRVCSGRFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLTFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(13417..13812)
                     /gene="hisI"
                     /locus_tag="WGR74_000746"
     CDS             complement(13417..13812)
                     /gene="hisI"
                     /locus_tag="WGR74_000746"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ0905204.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRILMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(13843..14610)
                     /gene="hisF"
                     /locus_tag="WGR74_000747"
     CDS             complement(13843..14610)
                     /gene="hisF"
                     /locus_tag="WGR74_000747"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ0905205.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNPGNTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGVFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(14623..15360)
                     /gene="hisA"
                     /locus_tag="WGR74_000748"
     CDS             complement(14623..15360)
                     /gene="hisA"
                     /locus_tag="WGR74_000748"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ0905206.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(15393..16031)
                     /gene="hisH"
                     /locus_tag="WGR74_000749"
     CDS             complement(15393..16031)
                     /gene="hisH"
                     /locus_tag="WGR74_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ0905207.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(16162..17664)
                     /gene="pta"
                     /locus_tag="WGR74_000750"
     CDS             complement(16162..17664)
                     /gene="pta"
                     /locus_tag="WGR74_000750"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ0905208.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRVNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVGPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(17849..18907)
                     /gene="fbpC"
                     /locus_tag="WGR74_000751"
     CDS             complement(17849..18907)
                     /gene="fbpC"
                     /locus_tag="WGR74_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ0905209.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(18928..20445)
                     /gene="fbpB"
                     /locus_tag="WGR74_000752"
     CDS             complement(18928..20445)
                     /gene="fbpB"
                     /locus_tag="WGR74_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ0905210.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLNLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAVLTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(20513..21508)
                     /gene="fbpA"
                     /locus_tag="WGR74_000753"
     CDS             complement(20513..21508)
                     /gene="fbpA"
                     /locus_tag="WGR74_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ0905211.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALITYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(21759..22228)
                     /locus_tag="WGR74_000754"
                     /pseudo
     CDS             complement(21759..22228)
                     /locus_tag="WGR74_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
     gene            complement(22285..23661)
                     /gene="argH"
                     /locus_tag="WGR74_000755"
     CDS             complement(22285..23661)
                     /gene="argH"
                     /locus_tag="WGR74_000755"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ0905212.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAELLG
                     FEQICQNSLDAVSDRDFAVEFTAAASLIMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGSAPEQVRFQV
                     KRWREMSA"
     gene            complement(23680..24549)
                     /gene="galU"
                     /locus_tag="WGR74_000756"
     CDS             complement(23680..24549)
                     /gene="galU"
                     /locus_tag="WGR74_000756"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ0905213.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(24577..25176)
                     /gene="rdgB"
                     /locus_tag="WGR74_000757"
     CDS             complement(24577..25176)
                     /gene="rdgB"
                     /locus_tag="WGR74_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ0905214.1"
                     /translation="MSEKTEKIVLASGNAGKLKEFGNLFKPYGITVLPQSAFGIPECP
                     EPYSTFVENALAKARHAAKYSGLPALADDSGICAAALNGAPGIHSARYAGDHPKSDTA
                     NNLKLAAELAGKADKSCCYVCVLVFVLHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALAELLRKLAL"
     gene            complement(25169..25402)
                     /locus_tag="WGR74_000758"
     CDS             complement(25169..25402)
                     /locus_tag="WGR74_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905215.1"
                     /translation="MNRRKIYLLSIALFTLAFMLLVLLGAYLLTAGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLRAAQSAAKENRYV"
     gene            complement(25510..26043)
                     /locus_tag="WGR74_000759"
     CDS             complement(25510..26043)
                     /locus_tag="WGR74_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ0905216.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(26145..26603)
                     /gene="nudB"
                     /locus_tag="WGR74_000760"
     CDS             complement(26145..26603)
                     /gene="nudB"
                     /locus_tag="WGR74_000760"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ0905217.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLAEGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPIALQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            26814..26890
                     /locus_tag="WGR74_000761"
     tRNA            26814..26890
                     /locus_tag="WGR74_000761"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26848..26850,aa:Pro,seq:ggg)
     gene            27048..28934
                     /gene="mafB"
                     /locus_tag="WGR74_000762"
     CDS             27048..28934
                     /gene="mafB"
                     /locus_tag="WGR74_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ0905218.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGKIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLANKRHPKTKVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAHAQREWENKTGLDFNHF
                     IGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVEIKKPDGSWEVKTKKGG
                     KVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNKWQGTSKSGIKIEGFTEPNRTAY
                     PIYE"
     gene            28965..29384
                     /locus_tag="WGR74_000763"
     CDS             28965..29384
                     /locus_tag="WGR74_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905219.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            29421..30395
                     /gene="mafB"
                     /locus_tag="WGR74_000764"
                     /pseudo
     CDS             29421..30395
                     /gene="mafB"
                     /locus_tag="WGR74_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
     gene            30406..30867
                     /locus_tag="WGR74_000765"
     CDS             30406..30867
                     /locus_tag="WGR74_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905220.1"
                     /translation="MNLETAENIQCEFEYLALDGYFPMHFASHGQGNKDWQFAVEFIY
                     RLLICQLATLEPINFETKNDILDFCHNLAKQSPFNNDNEVWYQGEIVLTKKGIDLIKE
                     YIPEAFEEWNGKKFELNIPFIKTLKNIFVNYEVAWDENNPLFPIISLNLGT"
     gene            30893..31141
                     /locus_tag="WGR74_000766"
     CDS             30893..31141
                     /locus_tag="WGR74_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ0905221.1"
                     /translation="MEIKKPDGSWEMKKGRGGKPMTNHSMFPKDWDEARIRAEVTSAW
                     EKRKELEGQKWRGTSKSGVEIEGFTEPNRTAYPLYNSK"
     gene            31168..31587
                     /locus_tag="WGR74_000767"
     CDS             31168..31587
                     /locus_tag="WGR74_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905222.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            31665..31748
                     /locus_tag="WGR74_000768"
                     /pseudo
     CDS             31665..31748
                     /locus_tag="WGR74_000768"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            31745..31987
                     /locus_tag="WGR74_000769"
     CDS             31745..31987
                     /locus_tag="WGR74_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ0905223.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            32065..32310
                     /locus_tag="WGR74_000770"
     CDS             32065..32310
                     /locus_tag="WGR74_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0905224.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PNYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            32313..32702
                     /locus_tag="WGR74_000771"
     CDS             32313..32702
                     /locus_tag="WGR74_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905225.1"
                     /translation="MWKIIKEDSNDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDGGIADIDNIVGFVSSYSLSKSKKNALTGIAFLRGIDVYDPPISKEK
                     ALKALKKHPEIYQRFQHFFPFVELPPL"
BASE COUNT         7700 a         7667 c         9262 g         8108 t
ORIGIN      
        1 cgttcgtacc cctttaaata cagctcaaaa tgcgctttgg gaatgccgtt aaacttgcgt
       61 aaatgacgtt ttgcccgatt ccaaaagttc tcaattccat taatatggtt ttgtcgttca
      121 gcaaaataac tttcatctgc ttctacttcg ccatcaaaca tttccaaatg tggactgttt
      181 tgataaataa gtaatcgtaa acgatgaaaa taataggctg cggtactttt attaacgcct
      241 actaactctg ctgctgttct tgcagttaca cctgcgacaa acagttcaat gagtttattt
      301 tgtttatacc ggcttagacg actttttctc ataggggcaa ctctaactta atttgaattt
      361 ccctagttat ctaggacagc cccaaattta tacaaaaatg agcgcggttc ggcgcaacct
      421 tgaatcaagt tcccgcatcg gttttcattg ccggtacgga tgcgttcaag ccggttttgc
      481 aaaggccgcg ctttcggcaa gcggacacgg acactgccga cggttgcgcc gttaacgggg
      541 ggagggagga gctgcgccga ccgtgtgaat gaaagtgccg tctgaaaccc gattttcagg
      601 cttcagacgg catttcgcat taatgcgggc ggcgcgttta tttgccgcgc atcagttcaa
      661 agaaatcgtc gttgttttta gaggctttga ttttcccgtt taagaactcg gtagcttcga
      721 tttcatccat cgggtgcagg aacttgcgta agagccacat acgttgcaac tggtcgttcg
      781 gcacaagcag ctcttcgcgg cgcgtgccgg atttgttgat gttgatggcg gggaagaggc
      841 gtttttccgc catacggcgg tcgaggtgca attccatatt gccggtgcct ttgaattctt
      901 cgtaaatcac atcgtccata cggctgccgg tttcaaccaa tgcggtggca atgatggtca
      961 gcgaaccgcc ttcttccacg ttgcgcgctg cgccgaagaa acgtttgggg cggtgcagcg
     1021 cgttggcatc gacaccgccg gtcaggattt tgcccgaggt ggggacgacg gtattgtagg
     1081 cgcgggcaag gcgggtaatc gaatccagca ggatgaccac gtcttttttg tgttctacca
     1141 tacgcttggc tttttcaagc accatttcgg caacttggac gtggcgttgc gccggttcgt
     1201 caaaggtgga ggaaaccact tcgccgcgga cggaacggct catttcggtt acttcttcgg
     1261 gacgctcgtc aatcaagagg acgatgagct cgacttcagg atagtttgcg gtaacggcgt
     1321 gggcaatatt ttgcaacatc acggttttac cgcttttggg cggggcaacc aagagggcgc
     1381 gctgaccttt gccgatgggg gaaatcaggt cgatagcacg tccggtcagg ttttcttcgg
     1441 actttaagtc gcgttccagc ttcaactgtt cggtcggaaa cagcggggtc aggttttcaa
     1501 acaggatttt atggcggcat acttccgggt ggtcgccgtt gatggtatca agcctgacca
     1561 gggcaaaata gcgttcgttg tcttttggga cgcgcacgct tccttcgatg gtgtcgcccg
     1621 tatgcaggtt gaagcggcgg atttgggtgg gtgagacata gatgtcgtcg ggaccggcaa
     1681 gataggacgt gtccgcgctg cggaggaagc cgaagccgtc gggcaggatt tcaagcgtgc
     1741 cggagcaggt gaaaccctcg ccttttttca tcatctggcg gacgatggca aatacgaggt
     1801 cttgtttgcg gaatcggttg gcgttttcga tgccgtgttc ttccgccaat tctaagagtt
     1861 tggaaatgtg cagggtttgt aattcggaga cgtgcataat aatgatgtat tttgaagagg
     1921 aaaaagacag gcagatgccg tctgaaagaa gaagctgacc gttgccggtt gctcggggaa
     1981 gggggaattg taggcagtcg gcgcgtgggt gtcaaatatt atcgcggacg gggcatcggc
     2041 agaaaatgcc gtctgagcgg agctgcttgg aaaaaaatac ccccgcgctt ttcaggctcg
     2101 ggggtatggg cattgattat ttgttcaatt cattcgccaa atacagccaa gtttcgatga
     2161 cggtatccgg gttcagggaa acgctttcga taccttcctc aaccagccat ttggcgaagt
     2221 ccggatggtc ggacgggcct tgaccgcaga tgccgacata tttgttctgc ttgcggcagg
     2281 cggagatggc aaggtgcagc atcactttga cggcagggtt gcgttcgtca aacgattcgg
     2341 ataccaagcc gctgtcgcgg tcgagaccga gggtcagttg ggtcatgtcg ttcgagccga
     2401 tggagaagcc gtcgaagtat tgcaggaatt gttccgccaa taccgcgttg ctcggcagct
     2461 cgcacatcat aatcaggcgc aggccgtttt tgccgcgttc caagccgttt tctttcaggg
     2521 ctttgacaac ggcttcggct tcgcccaaag tgcggacgaa cggaatcatg atttcaacgt
     2581 tggtcagacc catttcatcg cggacgcgtt tcaaggcttt gcattccaag gcgaaacagt
     2641 ctttgaagtt gtcggcgaca taacgcgccg cgccccggaa gcccaacatc gggttttctt
     2701 catgcggttc gtatacgttg ccgccgacca ggttggcgta ttcgttggat ttgaagtcgg
     2761 acatacggac gatggtttta cgcggataaa ccgatgcggc caatgtcgcc acgccttcgg
     2821 cgattttatc gacgtagaag tcgacagggg acgcgtaacc ggcgatacgg cgggtaattt
     2881 ccgcttttaa ttcgtcgtct tgtttgtcaa attccaacaa ggctttcggg tggataccga
     2941 tttggcggtt gatgataaat tccatacgcg ccaagccgat gccttcgctg ggcaggttgg
     3001 cgaagctgaa tgcgagttcg gggttgccga cgttcatcat gactttgacg ggtgctttgg
     3061 gcatattgtc caaggcgaca tcggtgatct gtacgtccaa caggccggca tagatgaagc
     3121 cggtatcgcc ttcggcgcag gatacggtca cttcctgacc gttttccaag agttcggtcg
     3181 cattgccgca gccgacgacg gcaggaatac ccagttcgcg cgcgatgatg gcggcgtggc
     3241 aggtacggcc gccgcggttg gtcacgatgg cggaagcacg tttcatcacc ggttcccaat
     3301 ccgggtcggt catatcggta acgagtacgt cgccggcttc gacggaatcc atctcggaag
     3361 catctttaat caggcgtacc ttgccctgac cgactttctg accgatggca cggccttcgc
     3421 ataatacggt tttatcgccg ttgatggcga agcggcgcag gttgcggctg ccttcttcct
     3481 gggatttgac ggtttcgggg cgggcttgca ggatgtagag tttgccgtcc aagccgtcgc
     3541 gtccccactc gatgtccatc gggcggccgt agtgtttttc gatggtcagt gcgtaatgcg
     3601 ccaactcggt gatttcttcg tcggtaatgg agaagcggtt gcggtcttct tcggggacat
     3661 cgacgttggt taccgatttg ccggcttcgg ctttgtcggt aaaaatcatt ttgatgtgtt
     3721 tcgaacccat ggttttacgc aggatggcgg gcttgcccgc tttgagcgtg ggtttgaaca
     3781 cataaaattc gtccgggttg accgcacctt gtacgacgtt ttcgcccaga ccgtaagagg
     3841 aggtaacaaa gacgacttga tcgtagccgg attcggtgtc gagggtgaac atcacacctg
     3901 atgcgccgct gtcggaacgc accatgcgtt gaacgccggc ggaaagggcg acgatatcgt
     3961 gttcgaagcc tttgtggaca cggtaggaaa tggcgcggtc gttgtacagg gaggcgaata
     4021 catggtgcat cgcttcttta acgttttcca atccgttgat gttcaagaag gtttcctgtt
     4081 ggccggcaaa tgatgcgtcg ggcaggtctt cggcggttgc ggaagagcgt acggcaacgg
     4141 aaatgtccgc gccgccggca tcggcaacca ttttgttcca tgccgcttcg atttcggcat
     4201 cgagctgttc ggggaagggt gtgtccaaaa tccattggcg gatttctttg ccgacgcgtg
     4261 ccagttcggc aacgtcttcg acatccaatt ttgccagtgc ggcggaaatg cgttcgctca
     4321 gaccgttgtg tgcgaggaat gcgcggtagg cttcggctgt ggtggcaaag ccgccgggga
     4381 cgcgaacgcc tttttcggtc agctgactga tcatttcgcc cagcgaggcg tttttaccgc
     4441 ccacgcgttc aacatctgtc atacgcaggt tttcaaacca aattacgtag ttgtcggcca
     4501 tttgtgtgtc caatccaaaa tatgttaaaa aagaaacaaa tccgcgtgct tattttaagc
     4561 gattcgttcc tctgctgtca tgtgttttat ccgttttaaa atcatgatgc cgtctgaaaa
     4621 attgcggttt cggcgtgtgt agcggtttga aacttgcagc cggtatactt ctttttttgg
     4681 gtattttctt tgtaaaacag gtggtttgaa taggttaatg ttttttctgt ttgatttttt
     4741 tgtttatttt ttaaaatttt ctgccaaaaa atactttata taaataattt tatttcaaaa
     4801 ttatattgtg tctgtttggg tgtaatccga ggtaggcgtg ctgcgggtgc tttccttgtg
     4861 tctgccgctg ctgttatgat gatattttta ttctgtattt aaggatggaa gatgagcagt
     4921 ccgcgccatg tgttttacat ttccgaccgt accggtctga ctgccgagaa tatcggcgag
     4981 gcgttgctga accagtttgg caatctgtcg ttcaaacgcc atacgcatcc gtttgtcgat
     5041 acgccggaaa aggcgcgcgc ggtggtggag aaggtcaatc ggagccggca ggaaaacggt
     5101 cagcgtccga ttgcgtttgt cagtgtggtt gatgacgaaa tccgtcggat tatcaaaggg
     5161 gcggatgctt ttcagattaa tttctttgag acttttttgg gactgttgga gaaggaactc
     5221 aataccgaag ccacggcatc cgagcagggg catcacagta tcggtaatac gaagcgttac
     5281 gatgcgcgta tggaggcggt aaatttctca ttgaaccacg atgacggggt cagcgataag
     5341 aaccttcagg aagcggatgt aatcttgatg ggtgtatcgc gttcgggcaa aacgccgacc
     5401 tgcctttacc tcgccctgca atacggcatc cgtgcggcaa actatccgct gattcccgac
     5461 gatttggaat cggccgatct gccgcgtatg gtcaagcctt atagggataa gctgttcggg
     5521 ttgaccatcc agccggaacg tttgcaggcc atccgccaag agcgccgccc gaattcaact
     5581 tatgccaaaa tcgatacatg ccgcagcgag gtggcagacg cgcagagtat gttcagacgg
     5641 catgggattc cgtttgcgaa tacgacggat aaatcagtgg aagagcttgc cgtacacatc
     5701 cttcaggcgt gcaagctcaa acgcaggttt tgacgggttt tgattcggtt tgaaggtgga
     5761 aatgccgtct gaaatcaggt ttcagacggc agttttatag tggattaaca aaaatcagga
     5821 caaggcgacg aagccgcaga cagtacaaat agtacggagc cgattcactt ggtgcttcag
     5881 caccttagag aatcgttctc tttgagctaa ggcgaggcaa cgctgtactg gtttagattt
     5941 aatccactat atgtttgtgg ggcggatatt tttcagggct gtattttgtc cagacattcg
     6001 agcagatcga gtggcgtgcg gatgtggaaa tccgcctgcc atgagccggt atcgtcttcg
     6061 ggagcgatgt agccccattc ggcgaggacg gttttcatgc cggcgttgcg gcctgcttgg
     6121 atgtcgcgtt cggcatcacc gacgtagagt gtgtgttgcg ggtcggcgtg gatttgtccg
     6181 cacgcataca gcatgggttt gatgctgggc ttgggttcgc cgcaggtgtc gccgctgacg
     6241 acgacggcgg gtgggatgat gaagccgagt ttggggacga gtttgtcggt gaagcgcatg
     6301 ggtttgttgg tgatgatgcc ccatttgatg ccgcgttggt tgagttcggc aatgagttcg
     6361 ttcacgccgt caaagagctc ggtgtcttgg gcgtagcggc ggtcgtattc ctccagatat
     6421 tcggtgcgcc atcgggcata gtcggggtgg tcgggggtga tgcctgcgcc gagtttgatc
     6481 agtcctgccg cgccgtggct ggcttgggtg cggatctcgt ccatgctttt tgcaggtagt
     6541 ccgtggcgag cgagcagggt gttgagtgcg cctccgaggt cgagggcggt gtcggcaagt
     6601 gtgccgtcga ggtcgaagag tacggcgtgt atcatgtgtg ttccttttca gacggtattt
     6661 ttgttctgtt tcaaaccctg ctcgaaatct tccagcatat ccaattcaaa gcggctgaag
     6721 cccgcttttt cgcgcgcttc gatgttcaca tagccgcgga agataaacat atcgtaacgg
     6781 gtaatcagac tgcggaacag ggcgacaggt tccaaaccgc gttcgcggca gaggtgttga
     6841 taccaatggt tgccgatggc gacatggccc acttcgtcgc ggtaaatgat gtccaatacg
     6901 ccgcaggttt ccgaatcgcc gcgctgcgcc accttcgcgc gtatccccgg cgtaacgtcc
     6961 agcccgcgcg cttccaacac gcgcggtacc aacgccatac gcaacagcgg gtcgtaggcg
     7021 gttttgtacg ccatatccca caaatggttg tgcgcctcga aactgccgta atcgaagccg
     7081 aaagcgcgca atctgtcgcg catcaggcgg aaatggtaca cctcttcctt cgccactttc
     7141 acccagtcgc ggacaaactg aaacggcagc gtgcggaaac ggtatgccgc gtccaaagcc
     7201 agattgacgg cgttgaattc gatatgcgca atcgcgtgca gcatcgccgc atagccttct
     7261 gccgtgttca ttttgcgtgg cgtcagttgc gacggcgcga ccaaaacagg cttgtccggc
     7321 cgtcctgcgt ggcggaagtc cagcggcggc gcgtttgcct ccgccccgtc aatgttttga
     7381 acggcggcaa acgcctcatc cgtcagctgt cctttttcat cggggtcgtc tgaaagcagg
     7441 gcaagttcca gcaaagcata aatatcgggt ttcatctcaa gtccgccgtg tttggaaaac
     7501 aaatattata gcgtttaaaa aaaacaagat gaggcatata atctccgcaa ttcggcattc
     7561 cgcgcccaaa ccgtcaaata acccggcaca catttaaagg gcggcgtggc acgcctgcct
     7621 tttctcgggg gcaaaaaaat cagccgtcgg aaaacgcggt ttgcaaaatg ccaaccgccc
     7681 gtcccaccgc ccgtatgatt gttttgccac gccgatactt tacgccacac ccgttccaac
     7741 aaggaaaaat aatgatgaaa ccgcacaaac tgttccaatt cctcgccgtc tgctccctga
     7801 ccgccgccgt cgcttccgca caggcgggcg cggtggacgc gctcaagcaa ttcaacaacg
     7861 atgcagacgg tatcagcgga agcttcaccc aaaccgtcca aagcaaaaag aaaacccaaa
     7921 ccgcgcacgg cacgttcaaa atcctgcgtc cgggcctttt caaatgggaa tacaccaaac
     7981 cttacaggca aaccatcgtc ggcgacggtc aaaccgtttg gctctatgat gttgatttgg
     8041 cgcaagtgac caagaccgcc caagaccgtg ccatcggcgg cagccccgcc gccatccttt
     8101 ccgacaaagc cgccctcgag gcaggttacg cccttgaaga agacggctcg tccggcggca
     8161 tcgactatgt tttggcaacg cccaagaaaa acaacgccgg ctaccaatac atccgcatcg
     8221 gcttcaaagg cggcaacctc gccgccatgc agctcaaaga cagcttcggc aaccaaacct
     8281 ccatcagctt cggcagcctg aacaccaagc ccggcctctc gcgcggcgcg ttcaagttta
     8341 ccccgcccaa aggcgtggac gtgttgagca actgatgccg tccgccccga tgccgtctga
     8401 acgccgccga ggcttcagac ggcattttta cgcaggcgga acaatgtccc gcattaccgc
     8461 ccgatcgggc atcggaacgg caaacccgtg aaaattaacg gttgcgcccc ggccgttttt
     8521 gccgtttaat gcaaaccttg ctgcactaag ggccaagaaa gccgaccggc cgcccccaca
     8581 gcttccgatg caggcggccc ggccgtccct gcaatgtttt ttatttttga acgaaaggtc
     8641 gaaaaccatg aaaaaaacac tggtggcggc ggcaatcctg agcctcgcct tgactgcgtg
     8701 cggcggcgga agcgataccg ccgcccaagc cccctccgcc aagcccgaag ccgaacaatc
     8761 gggcaaactc aatatctaca actggtcgga ttatgtcgat cccgaaaccg tcgccgcctt
     8821 tgaaaaagaa accggcatca agacgcgttc cgattattac gacagcaacg aaacactgga
     8881 ggcaaaagtc ctgaccggca aatccggcta cgacctgacc gcgccgtcca tcgccaacgt
     8941 cggccggcaa atcaaagcgg gcgcgtatca gaaaatcgac aaggcgcaaa ttccccatta
     9001 cggcaacatc gataaagatt tgttgaaaat gatggaagcc gtcgatccgg gcaacgaata
     9061 cgccgtcccc tatttctggg gcatcaacac cttggcaatc aacacgcggc aggtgcaaaa
     9121 ggcattgggt acggacaagc tgcccgaaaa cgaatgggat ttggtgttca aacccgaata
     9181 caccgccaaa ctcaaatcct gcggcatcag ctatttcgac agcgcaatcg aacagattcc
     9241 cttggcgttg cactatttgg gcaaagaccc caacagtgaa aatcccgaag acatcaaagc
     9301 cgccgtcgat atgatgaaag ccgtccgggg cgacgtgaaa cgcttcagct cttccggcta
     9361 tatcgacgat atggcggcgg gcaacctgtg tgccgccatc ggttacggcg gcgatttgaa
     9421 cattgccaaa acccgtgccg aagaagccgc aaacggcgtg gaaatcaaag tattgacccc
     9481 gaaaaccggc gtgggcgtgt gggtggattc ctttatgatt ccgcgcgacg cacaaaacgt
     9541 tgccaacgcc caccgctata tcgactacac gctccggccc gaggtggcgg cgaaaaacgg
     9601 cagcttcgtt acctacgcac ccgccagccg ccccgcgcgc gagctgatgg atgaaaaata
     9661 cacctccgat gcatcgattt tcccgaacaa agaactgatg gaaaaaagtt tcatcgtatc
     9721 gcccaaatcc gcagaatccg ccaaactggg cgtgaagctg tggcaagggc tcaaagcggg
     9781 caaataaccg gactccctgc cgtctgaaac ctttcgggcg gcaggaaacg gcgcgtccgt
     9841 tatcaaacag ggggcgtttc ccctcctgcc ggttatgatt gggttaagat taaaatgatt
     9901 tagtaaaatg agaaagatat ggatttaagt atcgtagttc ccatttataa tgtcgaaagt
     9961 tatttggaag cgtgtttaag ttccatagaa cctatattaa gtaatgaaaa tgtcgaactt
    10021 atccttgtga atgacgggtc aaaagacgga agtgaagata tatgttataa atatatagat
    10081 aaaacaacaa acaacaaaca acaaacaaca aacaacaaac aacaaacaac aaacaacaaa
    10141 caacaaacaa caaacaacaa acaacaccag ataccgaaca ccaaacatca aatatatata
    10201 tcaggataac caagggacgg cagaagctag gaataccgga ataaaaaatt caaacggaaa
    10261 atatatagtc tttattgatt cagatgattt tattaattgt caggtcttgc tggattttct
    10321 tagtaaagat gatattgata tgctggatgt ggtgttttta aatgcagtta aatatgataa
    10381 gggaagtgtt tcacattttg gcgaagatta tcagcctgaa aaaatactca atcaatccaa
    10441 agtcgaagtt ttgaaaggat tatgccgatt tagaaaattt ccgggttcgg cgtgtaataa
    10501 gattataaaa agggaattga ttatcaaaga aaaactgttt tttgaaaaag aggtttatgc
    10561 cgaagatatc gaatggtcaa tgagattatt taatgcggca acaacttttt cttatttgga
    10621 cggttgttat tactattatc ggcagggaag aaaagattct aaaacaggaa ctgtttcgga
    10681 aaaaagtata aagtcattat tatatatttt ggagaaaaat gcggaaatgg aatttaatag
    10741 ggatatatca agttatcttt attcctttct ttcctacgaa tatctcgttt tgctttttat
    10801 catgacgagt aaaaatatag agtgtgatgc tgatataaaa agaagggcgt atcatttaag
    10861 gtttatgctg ttaaagtcca ataaattgat atataagctg atattcccga taatcacatt
    10921 attcggggtc gatattacag gcagaatttt aaaagtaatc agagggaatg tttaataaat
    10981 cctttaacac tatacatctt accgaaggag gaaaaatgaa cgcaatccga actttccaaa
    11041 accgcacgcc cgaaatccac gaaagctgta tgatagacga agcgtgcgtc gtcatcggcg
    11101 aagtgtcgct tgccgaagac gtttccgtgt ggccgtgcgc cgtgttgcgc ggcgatgtga
    11161 acagcatcac cgtcggcgcg cgcagcaata tacaggacgg cagcgtcctg cacgtttccc
    11221 acaaaaccgc cgccaaaccc gaaggatcgc cgctggttat cggcgaagac gttaccgtgg
    11281 ggcacaaagt gatgctgcac ggctgccgaa tcggcaaccg cgtcttggtc ggtatgggca
    11341 gcaccgtttt ggacgatgct gtgattgagg acgaagtgat gattggcgcg ggcagcctcg
    11401 ttccgccgcg caaacgcttg gcgggcggct acctttatat cggttcgccg gtcagacaag
    11461 tgcgcgtgct gaccgatgag gaaaaagcct ttttgaaata ttccgccgcg cattatgtga
    11521 agctgtcgaa acagtacggg atgtgaaatc acatcggcgt tcttgcgtca gccccaaatt
    11581 catgcggacg ggatgcatcc gataacggta tccgatgcgc cttgattttg accgtctgcg
    11641 tttgagttgc aggcaaaaat gccgtctgaa gccctttttt cgggtttaga cggcatttta
    11701 tcgccgattg ctttttacag tttgaccgaa tgttcgcgcg tttcgtggaa cacgatgtcc
    11761 ggccaacgtt cttgcgtgag tcccagattc acgcggttag gggcgaggta ggcgaggttg
    11821 ccgcccgcgt cgatggcgag gttgcccgcg ttggcttttt caaattccgc cagttttttc
    11881 ttgtcgtcgc acgataccca gcgcgccgac cagatggatg cgctgtcgaa cacggcttct
    11941 acgccgtatt cgttggccag ccgcgaggta acgacttcaa attgcaacac gccgaccgcg
    12001 cccaaaatca aatccgcgcc gctcatcggt ttgaaaacct gtaccgcgcc ttcttcgccg
    12061 agttgttgca aacctttttg cagttgcttg attttcagcg ggtttttgat gcggacgctg
    12121 cggaacagtt cgggcgcgaa gaatgggatg ccggtaaacg tcagttgttc gccttcggag
    12181 aagctgtcgc cgatttggat gttgccgtgg tttgggatac cgataatgtc gccggcgtag
    12241 gcttcttcaa ccagctcgcg gtcgtgggac atgaaggtta ccacgctgga ggcggcgatt
    12301 tcgcggttga tacgcaggtg tttcatcttc ataccgcgct cgaatctgcc ggagcagacg
    12361 cgcaagaagg cgatacggtc gcggtgtttc ggatccatat tggcttggat tttaaagata
    12421 aatccggaaa acttagcctc gtccggctcg accatacgta cggtcgcgtc gcgcggtttc
    12481 ggcgcgggcg cccagtcaat taatgaattg aggatttcct gaataccgaa gttgttaatc
    12541 gcagagccga agaatacggg cgtgagttcg ccggcgagga attcgtcgag attaaactcg
    12601 ttcgatgctg cctgcaccaa ttcgatttcg tcgcgcaact gttggatttc caatggaaag
    12661 cgttgttcca attcgggatt ttctatgcct ttgatgatgt cgaactcgtg cggcaggcgt
    12721 tcgccgcccg cttcaaagag atagatttcg tcgttcagga tgtggtatac gcctttgaag
    12781 tttttgccca taccgatcgg ccaggtgacg ggcgcgcagc ggatttgcag gatgttttcc
    12841 acttcgtcca gcaattccag agaatcgcgt acttctcggt cgtatttgtt catgaacgta
    12901 acaatcggcg tatcgcgcag gcggcagacg tttaagagct tgatggtttg cgcttccacg
    12961 ccttttgccg cgtcgatgac catcaacgcg ctgtccacgg cggtcaaaac gcggtaggtg
    13021 tcttctgaga aatcttggtg tcccggcgtg tccaagaggt tgacggtgtg gtctttatag
    13081 tcgaactgca tcacgcttga tgccacggaa atgccgcgct gcttctcgat gtccatccag
    13141 tcggaagtgg cgaatttgcc ggtttttttg ccttttaccg tacccgcgct ttgaatcgca
    13201 cctgaaaaca gcaagagttt ttcagtcaac gtggttttac ctgcgtcagg gtgggagatg
    13261 atggcaaacg tgcggcggcg gcgcacttgg tcgaggattt cttgggacat ggttttcttt
    13321 gcaaaaaggt tcaggccgct tttcagacgg cccggacagt gtttgagacg gcgaaattgt
    13381 acaaaaaaat gcctgataat tcaatgttgg aggcggtcag tgcgtgctgc cgtaaatctc
    13441 tttttcgtct ttcaggacgg catcggcggt ttcccacgcg ctgccacgcc agactttgta
    13501 aaagcagctt tctcgcccgg tgtggcaggc gatgccgccg ttttgggcga tgagcatcac
    13561 aatggcgtcg ccgtcgcagt cgaggcgcag ttcgcggact ttttgcgtgt gtcccgactc
    13621 ttcgcccttc atccattgtt tttggcgcga acggctgtaa tagtgggcaa agccggtttc
    13681 gacggttttt tgcagggctt cggcgttcat ccacgccacc attaaaatac gtttggtttc
    13741 ggcatcttgg gcgatggcgc aaaccaaacc tttttcgtca aatttgacgg cttcaagcag
    13801 gtttttatcc atatttcctt tcagacggca tagtcgaggc ggtcagaggc gcacttcgat
    13861 gccggcttcg cgcatagcgc gtttggcttc gcggatggcg atttccccga aatggaaaac
    13921 gccggcggca agtacggcat cggctttgcc ttcggttatg ccttcaatca ggtgccggac
    13981 attgccgacc ccgccggagg cgatgacggg gatgtcgacg gcttcggcaa cggcgcgggt
    14041 cagcggcagg ttgaaaccct gtttcgtacc gtccctgtcc ataccggtaa gcaggatttc
    14101 gcccgcgccg cgtttttgca tttcgatcgc ccattccacc gcatccaaac cggtcggatt
    14161 gcgcccgccg tgggtaaaga tttcccagcg tgtgtttccg gggttgacgg ctttggcatc
    14221 gacggcggcg acgatggctt gcgaaccgaa aaatccggcg gcttcgtcaa tcaggtcggg
    14281 acgggtaacg gcggcggtgt tgatgctgac tttgtccgcg ccggcattga gcaggcggcg
    14341 gatgtcggca acggtgcgta cgccgccgcc gacggtcagg gggatgaaga cttgtccggc
    14401 aacctcttcg atgatgtgca ggatggtgtc gcggttgtcg gatgaggcgg tgatgtcgag
    14461 gaaggtcaat tcgtccgcgc cttcgccgtt gtagcgtttg gcggcttcga cggggtcgcc
    14521 cgcgtcgcgc aaaccgatga agttcacgcc tttgacgacg cgcccgtctt ttacgtcgag
    14581 acaggggatg atgcgttttg ccagtgccat aatcggatgc ctttagtcga gggaatctgc
    14641 cagttgctgc gcttgggcaa aatcgatgct gccctcgtaa atcgcgcggc cggtaatcgc
    14701 gcctgctacg ccatgttttt cggcggcaca cagggcgcgg atgtcgtcca agccggtcag
    14761 tccgccggag gcgatgacgg gaatgcggac ggcttgggcg agtttgaccg tcgcgtcgat
    14821 gttcacgccg ctcatcatac cgtcgcgccc gatgtcggtg tagatgatgc tgttgacacc
    14881 gtcgtcttca aagcgttttg ccaaatcaat tacatgatgc ccggttacgg ttgcccagcc
    14941 gtcgatggcg gccataccgt ctttggcatc cagcccgacg ataatcctgc cggggaaggc
    15001 tttgcacgcc tcgcgcacga agtcggggtt tttgactgcc gccgtgccga taatcacgtc
    15061 gtttaagccc aaatccaaat attgtccgat ggttttcaaa tcgcgtatgc cgccgccgag
    15121 ctgtacgggg atgtctttgg cgacggcggc aaggatgtct ttgatggcgg gcaggttttg
    15181 cggaacaccg gcaaacgcgc cgttcaaatc taccagatgc aggcggcgcg cgccttgttt
    15241 gaaccagtgc agcgcggttt cggcgggcga atcggaaaag acggtcgcct cttccatcag
    15301 cccttgtttc aggcggacgc agcgtccttc tttcaaatcg atggcgggta tcagcagcat
    15361 aatttttctc cttgtgcggg gccgtgtccg gcttaccagt ttaaaaagtt tttcaacatc
    15421 gtcagcccgg catcgtggct tttttcggtg tgaaattgcg tggcgaatac gttgtctttg
    15481 ccgacgatgc aggcaaacgg ggacgggtag tcgctttcgc ccaatatggt ttcgggattt
    15541 tcgggggcga aatagtagct gtggacgaag taaaaacgcg tgtcttgggg aatatcttta
    15601 aacagcgggt ggttttgggt ttggcgcacg gtgttccagc ccatatgcgg gactttcaga
    15661 cggcatccct gcgggtcgcg gaggtcgcgc tcaaagcgtc tgactttgcc gccgaaccag
    15721 cccaagccgt cggtgtttcc ttcttcactg tggtcgaata aaagttgcgc gccgacgcag
    15781 attccgaaaa agggtttgtt ttttaaggca tctttgactg cctcgtccag gccgccgcgc
    15841 gtcagtgccg ccatacagtc gggcatcgcg ccctgcccgg gaaaaatgac tttgtcggcg
    15901 cgggacacgc gttcggggtc gccgcttaaa aagatttcgg tattttttcc ggcaagctgc
    15961 cccgccgtcc ggacggattt caatacggaa tgcaggttgc ccataccgta atcgataatg
    16021 gcggtttgca tggcttcctc ctcttttttt gcaatatggc tgcgatttta acaaacaaat
    16081 gtgccgtgct gataaaaatg ccgtctgaaa acgggagtct gtcttcagac ggcatagggt
    16141 ttaaacccgg aaagccgttt gtcagccttc catttgtttt gcctgaacgg cagtcagggc
    16201 tatggtgaac acgatgtctt ccaccagcgc gccgcgcgac aggtcgttga ccggtttgcg
    16261 caggccttgc agcagcgggc cgacgcttaa gacgttggca ctgcgttgga cggctttata
    16321 ggtgcagttg ccggtgttca ggtcggggaa gaccaaaacg gttgcctgtc ctgccaccgg
    16381 gctgcccggg gctttggatt tgcccacgcc cggcacggtt gccgcatcat attgcagcgg
    16441 gccgtcgatg gcgaggtctg ggcgtttttc ccgggcaagt ttggttgctt cgatgacggt
    16501 atcgacatcg gggccgctgc cggagttgac ggtggagtag gaaatcatcg ccactttcgg
    16561 gtcgatgccg aaggcttttg cggaatcggc agactggatg gcgatgtcgg caagctgttg
    16621 cgcggtcggg ttcggattaa ccgcgcagtc gccgaagacg aggacttggt tgggcagcag
    16681 cataaagaat acgctggaca cgaggcttgc gcccggtgcg gttttaatca gttgcaaagc
    16741 ggggcggatg gtgttggcgg tggtgtgaac cgcaccggat accaaaccgt ccacatcatt
    16801 ttgcgccatc atcatcgtac cgagtaccac ggtgtcttgc agttgcttgc gcgcgtcttc
    16861 gggtgtcagg cctttggatt tgcgcagttc gcacatcggt ccgacgtatt gttcgaccaa
    16921 tgaggcggga tcgatgattt ccaaagagtc gggcaggctg ataccgcgtt ctttggcaac
    16981 ggcttcgact tcttcgcgtt tggcaagcag gacgcagcgg gcaatgcctt tttcgtggca
    17041 gatggcggcg gcttggacgg tgcggggttc tgcgccttca ggcaggacga tgcgtttgtc
    17101 ggcttggcgg gcgaagtcga tcaggttgta gcggaattgc gccggcgaca ggcgttttgc
    17161 ttcgcggcct gccaatacgg atacgtcttt cagcgcgtcg ctcgaaccga agaaggtcag
    17221 gccggttttt tcggctgccg cttcggcaac ggaggctgcc gcgccgtcca cgacaaaacc
    17281 ttccaatacg cccggcgcgg cggcgaagaa ctgtttggca aggttcaccc gatttgccag
    17341 ttcgtcggca tcggcgttgt cggaacggac ggcgaagacg gctgccgcgt caagggacaa
    17401 tgccagttcg acgtttttgc ctgcgaggta gattttgtcg gcatcgggcg cgatgccttc
    17461 gatgacgagg ttggcggcat cgagtgcggc aactttgccg accagtgcgt cgaaccagtc
    17521 gtcgcttttg ccttgcgcga gcagggtttc ggcggttgcg tcaacggctt ggaaaatttg
    17581 tgcgtccagt gcttttgcaa aggcttgtgc ggcggcggag gcgtccagtc cggcagatac
    17641 gggtacgatg agtacttttg ccatgatata tcctttcgta tgctgcggtg tgcggcatat
    17701 gtggttggaa ggggcggcat ataggcagaa acggctgcct gcgtgccgtg cgtgccgtgt
    17761 ttggcttgag gcgcgcaggt tgaatatagc aaacaaattc tgtttccaac aagataaata
    17821 tccgcaggtt tgtggatgct gccgcctttc agagggtatt tccggggaag aacagggcgg
    17881 gaccgtccaa atggaggacg gcggaaatgc cgtctgacag ggtgggggcg gaagggaggt
    17941 tgagcgtgag gacggtttgt ccgacccgga gactgatttc ggtatgccgc gctttgggcg
    18001 tggttttgag aaccacggcg tgaatggagg cggtgggtgc ggaatggggg tgaaggctga
    18061 actgttccgg acggatgagc agtgtgccgc gtgtgcctgc gggtgcgccg ctttgaacgg
    18121 gcaggcggcc caatccgcaa tcggcggtgc cgtcggcgtt gagcgcggcg gggaacacga
    18181 tgccttcgcc gataaacagg gcggcatcaa ggtcggcagg ttgtcggtac aattcgtgag
    18241 ggcttgcagt ttggaggatg cgcccctgtt tcatcacggc aatccggtcg gcgtattgca
    18301 gggcttcttc gcggtcgtgg ctgacgaaaa cggcagattt gccgttggcg cgcagggcgg
    18361 caatcatgtc ttcgcgaatc tggcggcgca actgttcgtc cagcgcgctg aagggttcgt
    18421 ccaacaaaat cagttcggga tcgggtgcga gggcgcgggc gagggcgacg cgctgttgct
    18481 gtccgcccga aagttcgtgc ggatagcgtc cggcaagttc ggaaatgccg gtcaattcca
    18541 acatagcttc gatgcgctgc cgctcttgcg ccgtcttgcc tttgccgttg cccagcccgt
    18601 aggcggtgtt gcggtaaacg gtcaggtggg ggaacagcac accttcctgt acgacataac
    18661 ccaaacggcg ttcgcggacg gggaggttgg tatttttcga gaagatggtt ctgccggaaa
    18721 gcgaaatttc gccaaaatcg ggttgttcaa aaccggcaag gcagcgtaaa agggtggttt
    18781 tgccgcagcc ggacgcgccg acgataaaga ggatttcgcc cgggtcgagg ctgagcgaaa
    18841 tgtcgtttaa aaccggggtg ttttgaaaac ttttggacag gtgtccgatg tgcagggcgg
    18901 cggtcatggc ggtacttcct caagctgtta tttgaaagcg tatttcttca gcaggaatac
    18961 ggggatgccg gaaaataata ccagcatcag cgcgtaaggg gtggcggcgg cgtattgtgc
    19021 gtccgatgtg tattcccaaa cggcggtgga gagtgtgtgg acatcgtcgg tggtcagcag
    19081 cagggtggcg gtcagctctt tcatcagttt gaggaagacg agtgcgaatg cggcggtaat
    19141 gccgggcagg atggacggca gtaccaacgt cctgaaaata aagaagtgtc cgcgccccaa
    19201 tgttgcgccg acctgttcca tcccttttgg gagttgttcc aaggaagtcc ttagggtggt
    19261 ttgcgccatc ggcaggtaaa gcatgaaata ggcaaggatg acgacgataa aggtttggta
    19321 aacggcaggg gtgtagttga tgctgaaata aaccaaggat agggcgataa ccaaaccggg
    19381 gacggcgtgc agtaaaaacg gcagcctgtc tatccaaacg gttaaaaaat tgcgatagcg
    19441 gaccgatgcc caaacaaggg gcaaggcaca taatatagtc aaaaccgcac ctaaagccga
    19501 tacgcttaag gaacggataa aggcatcaaa tacggatacg agcgcgaatg tgccggaagt
    19561 gccgaccatc atccaatgta tcaatacgcc aaaggggata ataatgccca aagtcaacaa
    19621 gctgcttaaa aaaacaatcg cgccaatctg accggggagg ttgagggttt tgacgggata
    19681 aggacgggca acgcctttgc cgctgtggta aatcttggct ttgccgcgaa atatgctttc
    19741 tccaaatacg acgatgccgc acaccgccat taaaacagcg gaaagcaggg cggcggtatt
    19801 gttgttgtag gacatttcgt attcttggaa aatggcggtg gtaaaagtgg ggtagttcaa
    19861 aatggatacc gcgccaaatt cgaccagcat atgcagggca atcagtaaca cgctgctgcc
    19921 gatggcgggt ttgagctggg ggaggatggc ggaaaaaaag gtttgcaggc ggcttttgcc
    19981 caaggacagg ctgacttctt cgtaagacag gctgatgcgt ttgagtgccg cctcgacggg
    20041 caggtaggcg agcgggaacg aggacaggct cataatcatc actgtccccc aaaagccttc
    20101 gacacggaag gtcaggctga tccaggtgaa acagctgaca aatgcgggga tgcacaaagg
    20161 cagggtgatt gccgtctgaa aaaaggtttt gccgaagaag cggtaacgtt ggaacaaaag
    20221 ggcgcaggca atgcccaaaa caatggaaat cagggtaacg cccgccatca tcgtcaaggt
    20281 gttggagagc aaatcccaca tacgcgggcg gaacaacagt tcgacggcgc ggttgatgcc
    20341 gacctgccac gaacgcatag cgacatataa aaaaggcagg gtaagcggta gggcaatcag
    20401 taggatgagg ccggtaagcc aaatgggtat ttttttagga gacatagtgt ttttcatcgg
    20461 caaaacgggc ggacagtata aatgtccacc cgtttgacaa tccgaaaacg gcttatttca
    20521 taccggcttg ctcaagcagc cgggtggcgt gttctttttc ggaaacagtg gtggcggaca
    20581 cttggggtgc ttccaacttg gcgatgggtt ccaaattgaa agtggatacc acgtgcggat
    20641 tcaaaggata ttcggcacgg acggcggtca gggcgcgctg tccttccttg ctggcgagga
    20701 aggcgacgaa ttttttcgcc tcatccttgt tttgggagga ttttaacacg gctgcgccgg
    20761 aataggtaat gagtgcgccg ggatctctgt ggcggacgaa attcaggcgg gtgtggacat
    20821 tttgtacgcc tttttcacgc gcgaaagcgt gccagtagta gttgttgatg agggcggcat
    20881 cgatttcgcc gttttcaacc gcttgaaggg cgacggagtt tttagcgtaa ggcttgccgt
    20941 attctttcag acctttgagc catttcaatg cggccgcttc gcctttcagt ttgacgatgg
    21001 cgacaacctg ttccaagaac gcgccggaag tgggggcgta accgatgcgg tttttccatt
    21061 tcggcgtggc gtaattcagg acggattttt ccaaatcttt ttcagacagt ttgcgggtgt
    21121 cgtaaacgac gacgcgcgaa cgtccgctca gtgccaccca gtcttttttg gcggcaaccg
    21181 gcacgccctt gccgcgtgtt tcgttgatgg tggaggcggg caggggctct aggaggttgg
    21241 ctgcggaaag ggtggcgagt gccgggattt gttcggaata gaatacgtcg gcggggcttc
    21301 ggctgccttc ttctttgatt tgaccggcaa gctggtcgcc tttggcgctg ttgagtttga
    21361 ctttgatgcc ggtagcccgg gtaaaggcat ctgcaacggc ttgtgccgct tctttgtgtt
    21421 ggccgttgta cacggtaatg tctgccagcg cgggggttgc ggcggtcagg gctgcggcaa
    21481 gcagtgcgta tcggatagat gttttcatat cgattttctc ctaatgaatg agagtgtaca
    21541 ccttgttaaa acataacggt gtgtagtgta ttccttcttc tttataaatg caaataatta
    21601 ttttttaaat ttgttattat ccgatccggt tatggtttgt tctgacttgt attttttccg
    21661 tgcatcgcgc ccgtaaggcg gaagcggcgg gcaatgcgtg gcggaatgtg ggtaaaggcg
    21721 gcattttgat ttgtcggaat gcttgagaac ccctctcttt aaaacagcgt ctgaacaggg
    21781 ttgccggaat agtattgcca tcccggcaga tacagtttgt cggggtcttg ccaatattgt
    21841 tcatccagac tgttcggcag tgaggcggtt ttgttgtcga gatgttttgt tatatcccac
    21901 atttctttca ggtttttacc ttccgattgg aggtggcgga tttcttttct caatgcgtct
    21961 gccgcccgat ggatcagcat tgcgctgtgt atcgcgccga ttttttcaga acggaacaag
    22021 tcttttcggc ggcgggaaaa ccccaattgc agacaaattg cagtatgccg ccgttgccga
    22081 tgtcggcttc cgcccgccaa atcaaaacca gctcctgttc acgtccgtcc atgctttcga
    22141 gtttgccgtc atgctgttca aaaagtttgt ctaccgcctg ccacatcatc tgttcgaagc
    22201 ggtcggcttg ggtatccgta tcggtcatgg tgttcttgtc ttttaaaaat gccgtctgaa
    22261 catttcttca gacggcatcg ggggttaagc tgacatttcc cgccagcgtt tgacttggaa
    22321 acggacttgt tcaggcgcgc taccgcccaa gtggttgcgc gcgtttaagc tgccttcggg
    22381 tgtcagcacg ccgtaaacgt cgtcggcaat caaatcgctg aaaccttgta agacttcgag
    22441 cggcagttcg ctcaaatcga cgccggcttc atcagcatgg cgcacggctt gggcgacgac
    22501 ttcgtgggca tcgcggaaag gcatgccttt ttttaccaag taatctgcca aatcggtggc
    22561 ggtagcgaag ccctgcatca cggcggcgcg catattgtcg ggtttgacgg ttacgccgcg
    22621 catcatatcg gcgtaaatcc gcaacgtgtc gataagcgtg tcggcggtgt cgaacaaggg
    22681 ttctttgtct tcctgattgt ctttgttgta cgccaagggt tgggatttca tcagggtaat
    22741 cagaccgata aggtgtccga tgacgcggcc ggatttgccg cgcacgagtt cgggcacgtc
    22801 gggatttttc ttctgcggca tgatggacga acctgtgcag aaacggtcgg cgatgtcgat
    22861 aaagccgaaa cgcggactca tccacaaaat caattcttcg gacaggcggc tcaggtgaac
    22921 cataatcagc gaggcggcgg cggtgaactc gacggcaaaa tcgcggtcgg atacggcatc
    22981 gagcgagttt tggcagattt gctcgaaacc cagtaattcg gcggtaattt cgcgccgaat
    23041 cgggtaggtc gtaccggcaa gggcggctgc gccgagcggc atacggttga cgcggcggcg
    23101 gcagtccgcc atccgttcgt tatcgcggcc gagcatttcg acgtaggcga gcatatggtg
    23161 tccgaagctg acgggctggg cgacttgcag gtgggtaaag ccgggcatga cggtttcggc
    23221 gttttgttcc gccaaatcca gcaatgccgt ctgaaggctt tgaatcaggc tttggataac
    23281 ggtaatctgg tcgcgcagcc aaaggcggat gtcggtggcg acttggtcgt tgcggctgcg
    23341 gccggtgtgc aggcgtttgc ctgcatcgcc gattttgtcg gtcaggcggc gttcgatgtt
    23401 catgtggaca tcttccaaat cggacgacca ttcgattttg ccgctgcgga tttcttcgag
    23461 gatttccgcc atgccccgac ggatgtccgc caaatcgcct tcgtccaaca cgccggtttc
    23521 tttcaacatt tgcgcgtgtg ccagcgagcc ttgaatgtcc cattcggcaa gccgtcggtc
    23581 gaaaccgatg gaggcggtgt attgtttgac gagttcggaa acgggttcgt tgaaacgtcc
    23641 ggaccaggtt ttgtcgtgca taaggattcc ttgatggggt tattcggtgc ggtatttttc
    23701 caaaaggcgg cggaagggtt cgccggtttc ggggtgtttc aggccgtagg cgacggtggc
    23761 ttcgaggtag cccagtttgc tgccgcagtc gtagcgcgtg ccttcaaagg ggtgcgccag
    23821 gacaaattcg tgatcgagca gcttggcgat gccgtctgta agctggattt cgttgcccgc
    23881 gccgcgcggc agtccggtca gtaagtcgaa aatgcgcggg gtaaggatgt agcgtccgac
    23941 gacggcaagg ttggagggcg cgtcttcggg cttgggtttt tcgacaatgc cggtaatgcg
    24001 ttggaactgt ttgagctgtt cggtttcgac gatgccgtat gagccggttt gcgatgcttc
    24061 aacggtttct acgcccaaaa tgctgttgcc gctgcgcccg tacacttcga ccatttgttt
    24121 gagcgcgcct ttgggggcat caatcaggtc gtcggcaagg ataacggcaa agggttcgtc
    24181 tccgatggcg gcgcgggcgc acaagacggc gtgtcccaag cccagtgctt ccgcctgacg
    24241 gatgtagagg caggtaatgt tcggcggcag gatgttgcgg acgtgttcca acaatttgtc
    24301 tttatggcgc atttccaact cggtttcgag ttcgtatgcc ttgtcgaaat ggtcttcgat
    24361 gctgcgtttg ttgcgtccgg taacaaacac catttccgtg cagccggctt ccacggcttc
    24421 ttctacggcg tattggatca gcggcttgtc gacgatgggc agcatttctt tcgggcttgc
    24481 cttggtggcg ggcaggaagc gtgttcccat ccctgcgacg gggaaaacgg ctttccgtat
    24541 gggtttcatt ctttttcctt tgtattgttt tgatgtttaa agggcgagtt tgcgtaagag
    24601 ttcggcaagt gcctgcgcgc ggtggctttc gcggtttttg acctccgaat ccaattcggc
    24661 ggcggttttg ccgtgtccgg gcagataaaa atacgggtcg taaccgaaac cgttttcacc
    24721 ggcgggaacg ccgttccatt gtccgtgcca tacgccttcg gcaataatcg gacgcgggtc
    24781 gtctttatgg aggacaaaaa ccaatacgca gacgtagcaa cagcttttgt cggctttgcc
    24841 ggcaagttcg gcggcaagtt tcaggttgtt ggcggtatcg gatttgggat ggtcgcccgc
    24901 gtaacgtgcg gaatggatgc ccggcgcgcc gtttagggcg gcggcacaga tgccgctgtc
    24961 gtcggcgagt gcgggcagcc cgctgtattt ggcggcatgg cgtgcttttg ccagcgcgtt
    25021 ttcgacaaag gtggaatagg gttcggggca ttcgggtatg ccgaatgcgg attgcggcaa
    25081 tacggtgatg ccgtaaggtt tgaataagtt gccgaattct ttgagcttgc cggcattgcc
    25141 gcttgccaaa acgatttttt ccgttttttc agacatagcg gttttccttt gcggcggatt
    25201 gggcggcgcg tagggatttg tgccgcaggt agagggcgag tgcgccgatt tgcccgaaca
    25261 gcgcgccgaa tgcgaaaagg aaggacgcga cggcgaaggc tttgctgccg gcggtcagca
    25321 gataagcacc caagaggacg agcagcataa atgccagtgt gaacagggca atagacaaca
    25381 gatagatttt tcggcggttc atggcgttcg gtcggaaacg gtatgttcgg attatatccg
    25441 attgggacgg tattccctag aggctggaaa aatgccgtct gaaacggcgt tcagacggca
    25501 tggcgggcgt tatgcctgtt tgttccaacg ttcgatggat tctttgatga cttttttcgc
    25561 ttcttccgca tcgccccacg attcgacttt ggtcgtacct gcttttttca ggtctttgta
    25621 atggttgaag tggaactcga tttgtttgat gagctgttgc ggcaaatcgg acaaagtttt
    25681 gtaggcgttg ccgttgttgc ggtcgtcggc aggaacgcag acgattttgt cgtctacttc
    25741 gccgtcgtca acgaatttca tcacgccgat aacgcgcgct tccaagaata cgccggttgc
    25801 caaaggttgt tcggtaacga gcagcacgtc caattcgtcg ccgtcttcgt ccaaagtttg
    25861 gggaatgaag ccgtagttgg tcggtttggc gaagatggcg ggttcgacgc ggtcgagttg
    25921 gaatgcggcg agtttgcggt tccactcgat tttgtggttg ctgccggcgg ggatttcgtt
    25981 gacgacgttg atgatgccgc cgtccacatc gccgggggtc aggatttggt tgaagtctgc
    26041 catttggttt cctttgtttg ggaaagtttg aagtttgaaa gtatagcaca aacgtccggc
    26101 tgaaaatgcg cccgatgccg tctgaaaggg tgtacgggcg cgtgttaccg tttgcccaaa
    26161 aacctgccca gttccagaat cgcgcgcctg ttggacgggg aaaacacttt ttccgccgct
    26221 tcttccaagc cgaaccagcc gtaggagacg tgttcctcgg gttgcagggc gatgggcgta
    26281 tcgcgcggga tttcggcaga gaagaggtgt tcgcggtttt caaacacgcc ttttggatag
    26341 cggtgccgcc agtggtggta gatttcgtaa accgtgctgt cgtgccagtc ttgaagctgt
    26401 ccttccgcca gcagtatgcc ggtttcttcc caaacttcgc gccttgccgt ttgggcgacg
    26461 gtttcgcccg gttcgaggct gccggttacc gactgccaaa atcctttcgg atgcgtgcgt
    26521 tcgatgagca ggatgccgcc gtccccgcta taaaggacga ccagtgcgga gacggggtat
    26581 ttgagcggtt ttgccatcgg catctttcgg cgggctgcgg taatgaaggg gcggattata
    26641 gcaaacgccg cacgttatgg cgtttatcct tttccgtatc cttttttctg cacggatggg
    26701 acgcgccggt gtttgccggt aaattttccg ttgtgtcaaa aagataaggg cggttgtgat
    26761 tttaatgctt gccaaagcgt cgggcggaaa ctataatccg aaacttatcg agtcggagtg
    26821 tggcgcagtc tggtagcgca cttgcatggg gtgcaagggg tcgaaggttc gaatcctttc
    26881 actccgacca aaaattccga aagccgcttt caaaagcggc ttttttgccg tccgtatgat
    26941 tatgatgtag agtacgcggc gacagacatt caaatgccgt ctgaaaaccg ttcagatggc
    27001 atctctttat cttagtttca ttccgtacca tcttaaggaa catcaaattg ggcatttccc
    27061 gcaaaatatc ccttattctg tccatactgg cagtgtgcct gccgatgcat gcacacgcct
    27121 cagatttggc aaacgattct tttatccggc aggttctcga ccgtcagcat ttcgaacccg
    27181 acgggaaata ccacctattc ggcagcaggg gggaacttgc cgagcgcagc ggccatatcg
    27241 gattgggaaa aatacaaagc catcagttgg gcaacctgat gattcaacag gcggccatta
    27301 aaggaaatat cggctacatt gtccgctttt ccgatcacgg gcacgaagtc cattccccct
    27361 tcgacaacca tgcctcacat tccgattctg atgaagccgg tagtcccgtt gacggattta
    27421 gcctttaccg catccattgg gacggatacg aacaccatcc cgctgacggc tatgacgggc
    27481 cacagggcgg cggctatccc gctcccaaag gcgcgaggga tatatacagc tacgacataa
    27541 aaggcgttgc ccaaaatatc cgcctcaacc tgaccgacaa ccgcagcacc ggacaacggc
    27601 ttgccgaccg tttccacaat gccggtagta tgctgacgca aggagtaggc gacggattca
    27661 aacgcgccac ccgatacagc cccgagctgg acagatcggg caatgccgcc gaagccttca
    27721 acggcactgc agatatcgtt aaaaacatca tcggcgcggc aggagaaatt gtcggcgcag
    27781 gcgatgccgt gcagggcata agcgaaggct caaacattgc tgtcatgcac ggcttgggtc
    27841 tgctttccac cgaaaacaag atggcgcgca tcaacgattt ggcagatatg gcgcaactca
    27901 aagactatgc cgcagcagcc atccgcgatt gggcagtcca aaaccccaat gccgcacaag
    27961 gcatagaagc cgtcagcaat atctttatgg cagccatccc catcaaaggg attggagctg
    28021 ttcggggaaa atacggcttg ggcggcatca cggcacatcc tatcaagcgg tcgcagatgg
    28081 gcgcgatcgc attgccgaaa gggaaatccg ccgtcagcga caattttgcc gatgcggcat
    28141 acgccaaata cccgtcccct taccattccc gaaatatccg ttcaaacttg gagcagcgtt
    28201 acggcaaaga aaacatcacc tcctcaaccg tgccgccgtc aaacggaaag aatgtgaaac
    28261 tggcaaacaa acgccacccg aagaccaaag tgccgtttga cggtaaaggg tttccgaatt
    28321 ttgagaagca cgtgaaatat gatacgaagc tcgatattca agaattatcg gggggcggta
    28381 tacctaaggc taagcctgtg tttgatgcga aaccgagatg ggaggttgat aggaagctta
    28441 ataaattgac aactcgtgag caggtggaga aaaatgttca ggaaacgaga agaaggagtc
    28501 agagtagtca gtttaaagcc catgcgcaac gagaatggga aaataaaaca gggttagatt
    28561 ttaatcattt tataggtggt gatatcaata agaaaggcac agtaacagga gggcatagtc
    28621 taacccgtgg tgatgtacgg gtgatacaac aaacctcggc acctgataaa catggggttt
    28681 atcaagcgac agtggaaatt aaaaagcctg atggaagttg ggaggtgaaa acgaaaaaag
    28741 gtgggaaagt gatgaccaag cacaccatgt tcccaaaaga ttgggatgag gctagaatta
    28801 gggctgaagt tacttcggct tgggaaagta gaataatgct taaggataat aaatggcagg
    28861 gtacaagtaa atcgggtatt aaaatagaag gatttaccga acctaataga acagcatatc
    28921 ccatttatga atagtaatat ttatgaaaaa ttaggagatt aatgatgaaa agaattaagt
    28981 gcttttgtga taaatttcca tcaggagata catttagaat gtgtatcatt ctggatgact
    29041 atgataatag ggttgattat tatgtaggaa tatatgatta cattacgtct accttaatga
    29101 gcgatattta ctatcgatcc acgattgatg agcatttcaa gattatagaa ttaatagaaa
    29161 ataatccaaa tgaaatttat gatgatggcg gtggtcaaca attttgccta gaatttcatc
    29221 atgataaggt cattttttat cacaatgaat ttgatgaaga agatggttat ccagtattaa
    29281 gctgttcgct gcatactttt aaaactgctt taattgcttg gaatgctttt ttgcaattgc
    29341 ctaaaagtat tcattcggtg gtggagactg tgattgagga ataagcataa ttagcttaat
    29401 gaatagaatc agcgatatag aaaggtagaa tcagatatat cccacctaaa aattactctc
    29461 cttcagcacc gctaccaaaa ggacctaata atggatatag tgccgggaat ttaagcaaaa
    29521 aagtcctgac tttagtttac ataaacggca aactcaaatt cgggccaagc agtctgccgt
    29581 cttcggcatt gaaagcacct gttcccattg tcggcaaggc tgaggctccg cccggaaaag
    29641 ctgtgcctgt cggcgaagcc cgcattcctt caaatccaaa agcggtaaaa ggaacaaccg
    29701 ttaccctgtc gcaggaacaa cgcctagcca acggagagct gataccgaaa ggctctgtgg
    29761 taacgtggtc ggacaatgcg gtgaaggttg tgcggcctga tgggaaaaag cggagattta
    29821 atagtcggga ttgggaacag aaggcattgc cgaaaccgca actaccgaaa gattacagag
    29881 atgacatcaa cataggtgga aataatccta gatttaatca gcaacggcat gaaaaaatcg
    29941 aatcccacaa cccaaacaga atagaggaga tacgccaacg taatgccgtt tcttctgcag
    30001 aaaggcaggc tgatgcatta aaaaggatgc aaacgtctgg ccgcacccgc ctgcctgggc
    30061 agccacaaaa aggaaaaccc ggcgctgcta gagaaatgtc ctcttcagac aatccgaatc
    30121 aaacagccga ggcttttgca cgtcgatatt taggcaggaa acctacacta atggaacgag
    30181 tcgaaggtga aaaattatac ggaggtaaat gtgtgggctg ttggaaagcg caaatgccgg
    30241 acggaacctt tgtggtgtat aggccagcag gtaatgcttc tatgaccaag ggaactactg
    30301 cgtctgttga tattaataac ccaacattaa ataacttaaa tattaattta aaaggcaagc
    30361 caacagagtt aaaatttaaa ttccctgcta gataaggatg ttttaatgaa tttagagaca
    30421 gcagaaaata ttcaatgcga atttgagtat ttagcattag atggttattt tccaatgcat
    30481 tttgcatcgc atggtcaagg aaacaaagat tggcaatttg cagttgaatt catatataga
    30541 ttattaatat gtcaattagc aactcttgaa cctatcaact ttgaaacgaa aaatgatatt
    30601 ttagactttt gccataattt agcaaaacaa agtcctttta ataatgataa tgaggtgtgg
    30661 tatcaggggg aaattgtatt aacaaaaaaa gggatagatt taataaagga atatattcca
    30721 gaggcgtttg aagagtggaa tggaaagaaa tttgaattaa acattccttt cattaaaaca
    30781 ttaaaaaata tatttgttaa ttatgaggta gcatgggatg agaataatcc actattcccc
    30841 attatatctt taaacctcgg cacctgataa acatggggtt tatcaagcga cagtggaaat
    30901 taaaaagcct gatggaagtt gggaaatgaa aaaaggaagg ggcggaaaac ctatgacaaa
    30961 tcattctatg ttcccaaaag attgggatga ggctagaatt agggctgaag ttacttcggc
    31021 ttgggagaaa agaaaggaat tagaaggtca aaaatggaga ggtacaagta aatctggtgt
    31081 tgaaatagaa ggatttacgg aacctaatcg aacagcttat ccactttata atagtaaatg
    31141 aagtattgat taaaaaggtg tttttaaatg tataaaataa aatgttttta tgatcagttt
    31201 agttctggtg aattatttaa ctactgccag tttttagata attcttctaa tcaaaagata
    31261 aatacacgag gaacggtgta tcaatatatt atttatgttt taacaggaga cctttatcta
    31321 caaaaagaca tagatgaaaa tttagaattt atccatcaag cagaaaataa tcccaatgaa
    31381 gtttattcag gaggtggaca aggtttttgc tgggatattt ctgctgaaaa agtagttttc
    31441 tatcacaacg aatttgatga agaagacggc tggcctgatt taagctgttc gctgcatact
    31501 tttaaaactg ctttaattgc ttggaatgct tttttgcaat tgcctaaaag tattcattcg
    31561 gtggtggaga ctgtgattga ggaataagca taattagctt aatgaataga atcagcgata
    31621 tagattggac tgcaaatcca cgcttatgcg ctgtgccatg attaacagga agagagcaac
    31681 ttggatgggc tagtagggat ggtaagcatt taaatatatc aattgatgga aagattacac
    31741 acaaatgact aatttaaaat tagatttcta ctctgaagtt attataaaag attcttgccc
    31801 taatgatttg ttagaaaatg gtgagactat taaaggaaaa aaaggagtgg tattaggtat
    31861 aagcgaagaa gatggtataa tctatggcta tactatttta ctttttgata taaaatattg
    31921 tatatatata gataagaaat atattatacc gactggaaaa aaattctctc gcgatgattt
    31981 ttattagcta atttaagtaa tttaagcaag ttttttagat actgaaactg gtaattatgt
    32041 ttatgtaagc ataaggagtt cagattgaag gattctacag ggcaaatgag gctggcaacc
    32101 aaggatttgg cggaagccat taaacgagga gaagtacgta gttctgcttt tacaacaaag
    32161 caactaaagg caatcgaaaa aggagaatct aaaataccaa attacacttg gcatcatcat
    32221 caagacactg gaaggatgca attgattaat gaagacttgc atcatgatac cggccatatc
    32281 ggttggagag cgatgagtaa aggaaagtaa ctatgtggaa aatcataaaa gaggatagta
    32341 atgatttagg atttgcaatt aaatgcttat tctctcagtc tattgattta aatgaattca
    32401 aattatggat tgaacaagta atacgagata tgcccatcga ggacatcccg ttttatattt
    32461 ttgatttggc ggattttgat gggggaattg ccgatattga caatattgta ggttttgttt
    32521 cgagttacag cctatcaaaa tcgaaaaaaa atgccttgac aggcattgcc ttcttaaggg
    32581 ggatagatgt ctatgatccg cctatttcaa aagaaaaagc attaaaagcc ttaaagaaac
    32641 atcctgaaat ttatcagagg tttcagcatt tctttccgtt tgtagagctg cccccgcttt
    32701 aaacagtcaa aataccgtct gaaagccatt tccgccg
//
LOCUS       DAWFLT010000017        31432 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWFLT010000017 DAWFLT010000000
VERSION     DAWFLT010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.017"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..968)
                     /locus_tag="WGR74_000772"
     CDS             complement(<1..968)
                     /locus_tag="WGR74_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ0905226.1"
                     /translation="MAGNEKYLDWAREVLHTEAEGLREIAADLDENFALAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAAR"
     gene            1065..2120
                     /gene="tal"
                     /locus_tag="WGR74_000773"
     CDS             1065..2120
                     /gene="tal"
                     /locus_tag="WGR74_000773"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ0905227.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQDLTPKQRYETMAVADVRAACGVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDSALDTTLPDRLK
                     GKIAIALAKAAYQDWEQYFTAPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLATRLQED
                     GLKQFEEAFEKLLAPLV"
     gene            2262..2552
                     /locus_tag="WGR74_000774"
     CDS             2262..2552
                     /locus_tag="WGR74_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905228.1"
                     /translation="MAKALEIISPNEIYSDLIFKNPVPPHTIYTELMKLIGRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALIHSLK"
     gene            2569..3456
                     /gene="gluQRS"
                     /locus_tag="WGR74_000775"
     CDS             2569..3456
                     /gene="gluQRS"
                     /locus_tag="WGR74_000775"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ0905229.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAAASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSHRYALYEETLCRLQTAGLVYPCHCSRK
                     DWQAGARRGADGFVYNGRCRHPCQRPAPQGKQPAWRIRVPDRIIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLDVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLNLPEAPEADRPAELLD
                     WAVAHWDMGKVPKHAITAP"
     gene            complement(3526..4536)
                     /gene="dusA"
                     /locus_tag="WGR74_000776"
     CDS             complement(3526..4536)
                     /gene="dusA"
                     /locus_tag="WGR74_000776"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ0905230.1"
                     /translation="MNDNTQTLPPRRLSVAPMLDWTDRHYRYMARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGCPS
                     PRVQKGAFGACLMNEVGLVADCLNAMQDAVKIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EEIAQHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKNARTWRRMLSDATLLKDNDGGLILEAWKEVERANM
                     RE"
     gene            complement(4567..5058)
                     /locus_tag="WGR74_000777"
     CDS             complement(4567..5058)
                     /locus_tag="WGR74_000777"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ0905231.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DAHYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADATSGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(5132..5890)
                     /locus_tag="WGR74_000778"
     CDS             complement(5132..5890)
                     /locus_tag="WGR74_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0905232.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAHQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            complement(5961..6827)
                     /locus_tag="WGR74_000779"
     CDS             complement(5961..6827)
                     /locus_tag="WGR74_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0905233.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(6876..7154)
                     /locus_tag="WGR74_000780"
     CDS             complement(6876..7154)
                     /locus_tag="WGR74_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0905234.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(7154..7444)
                     /locus_tag="WGR74_000781"
     CDS             complement(7154..7444)
                     /locus_tag="WGR74_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ0905235.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(7447..7977)
                     /locus_tag="WGR74_000782"
     CDS             complement(7447..7977)
                     /locus_tag="WGR74_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ0905236.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLSDAVWAKLTYMWVGFLIFMGIANWFVFTRFEAQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            8285..10810
                     /locus_tag="WGR74_000783"
     CDS             8285..10810
                     /locus_tag="WGR74_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ0905237.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     DGLFGGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEI
                     TFAEVETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLA
                     AAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESV
                     AQTAENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTV
                     AEVQTPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENVTETV
                     AETPDFNATADDLSALLQPSEVPAVEENAAEIVADDLSALLQPAEAPAVEENVTKTVA
                     EIPDFNATADDLSALLQPSEVPAVEENAAETVADDLSALLQPAEAPAVEENAAEITLE
                     TPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNNADTSFPSESVGSDAPSEAKY
                     DLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            complement(10983..11459)
                     /gene="gloA"
                     /locus_tag="WGR74_000784"
     CDS             complement(10983..11459)
                     /gene="gloA"
                     /locus_tag="WGR74_000784"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ0905238.1"
                     /translation="MADVYAKQNRSLTTTERNTKMRLLHTMLRVGNLEKSLDFYQNVL
                     GMKLLRRKDYPEGRFTLAFVGYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDA
                     YEACERVKRQGGNVVREAGPMKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(11423..12592)
                     /gene="lapB"
                     /locus_tag="WGR74_000785"
     CDS             complement(11423..12592)
                     /gene="lapB"
                     /locus_tag="WGR74_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ0905239.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(12603..12917)
                     /locus_tag="WGR74_000786"
     CDS             complement(12603..12917)
                     /locus_tag="WGR74_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ0905240.1"
                     /translation="MKLIYTVVKIIILLFFLLLAVINTDAVTFSYLPGQKVDLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            13227..14225
                     /gene="ilvE"
                     /locus_tag="WGR74_000787"
     CDS             13227..14225
                     /gene="ilvE"
                     /locus_tag="WGR74_000787"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ0905241.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALIE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(14469..15254)
                     /gene="fabI"
                     /locus_tag="WGR74_000788"
     CDS             complement(14469..15254)
                     /gene="fabI"
                     /locus_tag="WGR74_000788"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ0905242.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(15409..16230)
                     /gene="dapD"
                     /locus_tag="WGR74_000789"
     CDS             complement(15409..16230)
                     /gene="dapD"
                     /locus_tag="WGR74_000789"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ0905243.1"
                     /translation="MSLQNIIETAFENRADITPSTVAPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(16240..16470)
                     /locus_tag="WGR74_000790"
     CDS             complement(16240..16470)
                     /locus_tag="WGR74_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905244.1"
                     /translation="MPSERRRRGIKVPPPFCIDSGAEKAIPAARPIPKRQCSATARVL
                     LTNMRLRGTIAHSFQMNILLENTGKRPTKHLT"
     gene            complement(16440..18083)
                     /gene="pgi"
                     /locus_tag="WGR74_000791"
     CDS             complement(16440..18083)
                     /gene="pgi"
                     /locus_tag="WGR74_000791"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0905245.1"
                     /translation="MDAFTRAWCALERHYQDTRHILLRDRFAAEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCNLADAADLEGKMRALRTGAKVNNSEGRAALHTALRLPDGA
                     GAVYADGRDVLPEIRRELNRALKFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPVWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLERLTPYNLGMLMAAYEHKTFVQGVIWDINPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            18593..19825
                     /gene="pilG"
                     /locus_tag="WGR74_000792"
     CDS             18593..19825
                     /gene="pilG"
                     /locus_tag="WGR74_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ0905246.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            19899..20759
                     /locus_tag="WGR74_000793"
     CDS             19899..20759
                     /locus_tag="WGR74_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ0905247.1"
                     /translation="MSDLSVLSPFAVPLTAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            20761..21393
                     /locus_tag="WGR74_000794"
     CDS             20761..21393
                     /locus_tag="WGR74_000794"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ0905248.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAVYGIVEIPLLTEKRQFISLIRRVLTISAPVEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHND"
     gene            21386..21595
                     /locus_tag="WGR74_000795"
     CDS             21386..21595
                     /locus_tag="WGR74_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ0905249.1"
                     /translation="MTESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSSQTESLPEISEPDMAYR"
     gene            complement(21680..23356)
                     /gene="pilB"
                     /locus_tag="WGR74_000796"
     CDS             complement(21680..23356)
                     /gene="pilB"
                     /locus_tag="WGR74_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ0905250.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
     gene            complement(23497..23559)
                     /locus_tag="WGR74_000797"
                     /pseudo
     CDS             complement(23497..23559)
                     /locus_tag="WGR74_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23757..23828)
                     /locus_tag="WGR74_000798"
     tRNA            complement(23757..23828)
                     /locus_tag="WGR74_000798"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23793..23795),aa:Arg,seq:cct)
     gene            complement(23848..24294)
                     /locus_tag="WGR74_000799"
     CDS             complement(23848..24294)
                     /locus_tag="WGR74_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ0905251.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(24299..25273)
                     /gene="ispB"
                     /locus_tag="WGR74_000800"
     CDS             complement(24299..25273)
                     /gene="ispB"
                     /locus_tag="WGR74_000800"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ0905252.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(25289..25477)
                     /locus_tag="WGR74_000801"
     CDS             complement(25289..25477)
                     /locus_tag="WGR74_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905253.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            25497..25805
                     /gene="rplU"
                     /locus_tag="WGR74_000802"
     CDS             25497..25805
                     /gene="rplU"
                     /locus_tag="WGR74_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ0905254.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            25830..26102
                     /gene="rpmA"
                     /locus_tag="WGR74_000803"
     CDS             25830..26102
                     /gene="rpmA"
                     /locus_tag="WGR74_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ0905255.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            26441..27625
                     /gene="ubiM"
                     /locus_tag="WGR74_000804"
     CDS             26441..27625
                     /gene="ubiM"
                     /locus_tag="WGR74_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ0905256.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIECSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNITILTDTAVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAQPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(27896..28051)
                     /gene="rpmG"
                     /locus_tag="WGR74_000805"
     CDS             complement(27896..28051)
                     /gene="rpmG"
                     /locus_tag="WGR74_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ0905257.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(28083..28316)
                     /gene="rpmB"
                     /locus_tag="WGR74_000806"
     CDS             complement(28083..28316)
                     /gene="rpmB"
                     /locus_tag="WGR74_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ0905258.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(28566..30092)
                     /gene="farB"
                     /locus_tag="WGR74_000807"
     CDS             complement(28566..30092)
                     /gene="farB"
                     /locus_tag="WGR74_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ0905259.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKCRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGIIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(30116..31270)
                     /gene="farA"
                     /locus_tag="WGR74_000808"
     CDS             complement(30116..31270)
                     /gene="farA"
                     /locus_tag="WGR74_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ0905260.1"
                     /translation="MDTHTDETKLQNTQAKRKRRLTALTLLFALAAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQRTQIRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
BASE COUNT         7620 a         8910 c         7878 g         7024 t
ORIGIN      
        1 atccgcgccg ccaaaaggtc gtgcatattc agcgcgccga tcagcacgcc atctgcatcg
       61 gtaaccagaa gcccgttcac atggtttgcc tgcatgactt tcagggcttc ggtggcgaga
      121 cgttcggcgg agatggtttt agggtgtgta tgcatgactt cgtctatcga aagaccggta
      181 aaattgtcgc attcttgaaa caggcggcgc aaatcgccgt cggtgaatac gcctttcaga
      241 cggccttgcc cgtccgttac cgccaacatg cccagccctt tttcactcat gctgacgatg
      301 gcttctttca agggcgtgcc gagtcggacg gcaggcaggc cgccgccttt gtgcataatg
      361 tcggcaacgc gcaaaagtag gcgtttgccg aggctgccgg cagggtggct caaggcgaag
      421 tcgtcgggcg tgaacgcgcg ggcgcgcagc aggacaaccg ccaacgcatc gcccaaagcc
      481 ataacggcgg tggtgctggt ggtcggggca agccccagcg ggcaggcttc tttggaaacc
      541 gacgccgtga tgtggatgtc ggcatggcgc gccatggttg aatcggggcg ggcggtgatg
      601 cagacaagcg tgatatcttt gcgtttgagc gcggggatga tggcggcgat ttcgtcgctt
      661 tcaccggaat tggaaatcgc gacgaccacg tcgttgtcca caatcatgcc caaatcgccg
      721 tgtgccgctt ccgcagggtg gacgaaaaac gcgggcgtgc cggtcgaggc catggttgcc
      781 gccattttgc gcccgatatg tcccgacttg cccatgcccg tgataacgac cctgcctttg
      841 cagtgcaaca acgcgtccgc cgcaagggcg aagttttcgt ccaaatccgc cgcaatttcg
      901 cgcaagcctt ccgcttcggt gtgcaacact tcgcgtgccc aatcaagata tttttcgttt
      961 cctgccatcg ccatccgcct ttgctgtaat aaaactaccc aaaatttccg aatcggggta
     1021 gcataagcct ttgaaacata cacatccatc caaaggaatc agaaatgact attttatcgg
     1081 acgttaaagc attaggacaa caaatctggc tggacaacct ttcccgctcg ctcgtgcaaa
     1141 gcggcgaatt ggcgcaaatg ttgaaacaag gcgtgtgcgg cgtaacttcc aatcccgcca
     1201 tctttcaaaa agccttcgcc ggcgatgcgc tttacgccga cgaggttgcc gccctcaagc
     1261 agcaagacct gacccccaaa caacgctacg aaacgatggc ggttgccgat gtgcgtgccg
     1321 cctgcggcgt ctgtcttgcc gaacacgaat ccaccggcgg caaaaccggc ttcgtcagcc
     1381 tcgaagtttc gcccgaattg tccaaagacg cgcaaggcac ggttgaagaa gcgcgccgcc
     1441 tctacgccgc catcgggtgc aaaaacgcga tgattaaagt gcctgccacc gacgcaggca
     1501 tcgatgcgct cgaaaccctc gtttcggacg gcatcagcgt gaacctgacc ctgctgttct
     1561 cacgcgccca aaccctcaaa gcctacgccg cctacgcgcg cggcattgcc aaacgcttgg
     1621 cagccggaca aagcgttgcc catatccacg tcgtcgccag cttcttcatc tcgcgtgtgg
     1681 acagtgcgct ggacacaaca ctgcccgacc ggctcaaagg caaaatcgcc atcgcccttg
     1741 ccaaagccgc ctatcaagat tgggaacaat atttcaccgc ccccgaattt gccgcactgg
     1801 aagcccaagg cgcaaaccgc gtgcagcttt tatgggcatc taccggcgtg aaaaacccag
     1861 cctatcccga cacgctctac gttgacagcc tgatcggcgc gcacaccgtc aacaccgttc
     1921 ccgatgccac gctcaaagcc tttatcgacc acggcacagc caaagccacg ctgaccgaag
     1981 gcgtagaaga agcacaagcg cagctcgccg aaaccgccgc gctcggcatc gatgtcgaaa
     2041 ccttggcaac gcgtttgcag gaagacggtt tgaaacagtt tgaagaagcg tttgaaaaac
     2101 tgctcgcacc gttggtttaa acttttcatc cccgatgccg tctgaaaggc tttcagacgg
     2161 catcgccctt ttgaccggac acagtgggtt caccctaaaa atattaatca acaagaagtt
     2221 aatgcacgac attacccacc aacacaaaag gaaatagaat catggcaaaa gcactcgaaa
     2281 tcatttcacc caatgaaatt tattcagatc taatttttaa gaatccggta cctccccata
     2341 ctatatatac agaattgatg aaactgattg gtagaaatgc aagtaatgag agaatcgcag
     2401 atagagcctt cgattttttc tcgtccggcc ttatggatgg ttcagctacc gaagagcaat
     2461 ataatgctct atatgattta accttgcttg aagagcccgg gatggaactg aataaagatg
     2521 agataatggc tttgattcat agtcttaaat aaaggggctg taccggatat gtacacagga
     2581 cgtttcgccc ccagcccgac cgggctgctc cacatcggct cgctgctgac cgccgccgct
     2641 tcctatgccg atgcacgctc aaacggcggc aaatggctgg tccgcatgga agacctcgat
     2701 ccgccgcgcg aaatgccggg ggcggcaagc catatcctgc acacgcttga ggcattcgga
     2761 ttcgagtggg acggagaagt cgcctatcag agccaccgtt acgccctgta tgaagaaacc
     2821 ctatgccgtc tgcaaaccgc cggactggtc tatccctgcc attgcagccg caaagactgg
     2881 caggccgggg caaggcgggg cgcagacgga ttcgtctata acggacgttg ccgccacccc
     2941 tgccaacgcc ctgcaccgca aggcaaacag ccggcgtggc gcatccgcgt ccccgaccgc
     3001 attatcggtt tttcagacgg catcgtcggc ggttacgccc aaaacctcgc ccgcgacatc
     3061 ggcgatttcg tcctgcttcg tgcagacggt tactgggcat accaactcgc cgtcgttgcc
     3121 gacgatgccg aacagggcgt tacccacatc gtccgcggac aagacctgct tgtttctacg
     3181 ccgcgccaaa tctatttgca gcagtgtttg gacgttccga caccgcaata tgcccacctg
     3241 ccgctgctga ccaacgcaca agggcagaaa tggtcgaaac agacgctcgc acccgcattg
     3301 gatttaaacc gccgcgaaca actcctccgc caagtgttcc gttacctcaa cctgcccgaa
     3361 gcaccggaag ccgaccgccc tgccgaactg ctcgactggg cggtggcaca ctgggatatg
     3421 ggcaaagtgc cgaaacacgc cattaccgcc ccctaaacac cgcccgacaa acgcacaaat
     3481 gccgtctgaa accttcagac ggcatttcat acacacagcc ccgccctatt cgcgcatatt
     3541 tgcccgttcg acttctttcc acgcttcgag aatcaggccg ccgtcgttgt ctttcaagag
     3601 cgttgcatcg gaaagcatac gccgccaagt gcgggcattt ttcagaccgt gcatcagccc
     3661 gaggctgtgg cggacgatgt ggcgcaagat tgtgccgcgt ccggcttgga tttgggcttg
     3721 actgtatgtg tagagacgct gcaccaaatc ggcgtattca atcgggctgc gggtatcgcc
     3781 gtaaaacagc ctgtcccatt cgcgcatcac catcgggttg tggtacgcct cgcgcccgac
     3841 catcacgccg tcaacgtgtt gcaggtgttg cgcgatttcc tcattggtcg tgatgccgcc
     3901 gttgatgatg atttccagcc cgggaaactc ttgcttgagg cggtaaacat aatcgtattt
     3961 caagggcgga acgtcgcggt tttctttggg tgaaagaccg tccagccacg cgttacgtgc
     4021 gtggacgata aaggttttgc aggcggtttt gtcgcgcagc gtgccgacga aatcggcaac
     4081 ggtttggtat tcggtctgcc tgtccacacc gatgcggtgt ttgacggtaa cgggaatctt
     4141 gaccgcatcc tgcatggcgt tgaggcagtc ggcaaccagc ccgacttcgt tcatcagaca
     4201 cgcgccgaac gcgcctttct ggacgcgcgg actggggcag ccgcagttga ggttgacctc
     4261 gttgtagccg tattcttcgg cggctttggc ggctttcgcc aaatcggacg ggtcgctgcc
     4321 gcccagttgc agggcgacgg gctgctcgcc ttcgttaaac atcaaaaagc ggtctttgtc
     4381 gccgtaaaca atcgcgccgg cgttgaccat ttcgctgtac agccaagtat tgcgggtaat
     4441 ctggcgcgcc atataacggt agtgcctgtc cgtccagtcc aacatcgggg cgacggacag
     4501 gcggcgggga ggaagggttt gcgtgttgtc gttcattgtc gatatttcac cattgcggat
     4561 tcaatttcaa tccggaacaa ccttcatttt tggggaaatc cgcgtcatga aagagtccag
     4621 cagtatggtt accggcccgt cgttgcagag cgacacctgc atatgcgtgc ggaaacgccc
     4681 tgtttcgaca tgaattccgt gtccgcgcaa cagttccgcc gttcgcaggt aaagctgctg
     4741 cgcctgttct gcaggtgcgg cttgcgaaaa cgaaggccgc cgcccgcttg tcgcgtcggc
     4801 ataaagtgta aactgcgaca ccagcagcac cgcgccgccg acatctttca aagacaggtt
     4861 cagcttgccc gcttcgtctt caaacacgcg caaatgggcg attttgtcgg cgatatagtg
     4921 cgcatctttt tctgtgtcgc tatgcgttac gccgagtaac acgacaaacc cgccgtcgat
     4981 tttgccacag gtttccgtgc cggcttcgga cacgacatcc acctttgcac ctaccgtttt
     5041 ctgtatgacc gcacgcataa gccgtcctcc ttacctgaac gtgcaatgcc gtctgaaaag
     5101 gcttcagacg gcatcggtat tggaaatgcc attacggttt gacaccgttt tcttccaaaa
     5161 tggcatcgat tttcaagcgg gctttttcct gtctcaaacc ttgttccaac tggtttctga
     5221 ccaactcgaa aggctgcgcg tcggggtttt tcccgacctc gctgagtttg aacagataat
     5281 agcgttcgcc caatttgacc ggatcgcggg taacgtcgcc ccgattcatc gctgcaaact
     5341 gcgaagccag cggctcggga agctgctgcg ccataatgaa accgtcaaaa gcctgctcgt
     5401 cgttcggata acgcttcatc agcccttcaa aagacagccc tttgagcagg agctgatgcg
     5461 cctgacgcgc ctcctcttcg gttgcgaagc tgacctgctg caatttgatc atgcggattt
     5521 gccgctcata aaactgacgc agtgcgcttt cggaaaccgt ttccgaacgt tccagaaaac
     5581 ggacgtactc ctcggcataa aaagacgctt cggcgatttt aaagcggttt tggacatcct
     5641 tatccttatc caaaccttcc ttcaatgccc tgtttttcaa aacttccaaa gtttgcagcc
     5701 gacggacggc atcgtttcgg attgcctgcc cgtccggttt ttgggactgc tccgcatgcc
     5761 ggtctgcctg ctgcatgatc tgcgccacca gcgtatccac caaagccggg tcgatttcgg
     5821 gtgctttggc tgccgcaaaa cctgccaaca ttgcagcaat aactgcggca gcggtttttt
     5881 tctgtttcat ggtattccct gtcctgcgcg gcggcaaaac gcctgtctga acgtcttgcc
     5941 gccatatccc gttttcagaa ttattttgca ggtttgatgt ttgccttgcc caacagtgca
     6001 ccgacggcac ggtcaatccg ttccgcctga aggttgccgg caatctgtcc tttcatttca
     6061 tcaaaagaag gcacttttac ctcgcggctg tcgttgacat aataaacgcc gtagaaatcg
     6121 ccgtttttca gcggcgttgc cgtaaattcg ccttttttca agtccttaat tgcctgataa
     6181 agcggcggaa caccctgttc caaatctttc agcggcacat atccgaccgg cgcgccggtc
     6241 tgtttggtac ggtcgttgag ggaatattgt ttcaagacgg catcgaaacc ttttttcgcc
     6301 ttcaagtcgg caaccgcttt ttttgcattt tcttccttgt cggtcaggat ttcgcccaac
     6361 tggacttcct gcgtaccttt gtaaaaaccg ctgatattgt catatgcggc ttttacttcc
     6421 tgctcggaaa ccggttgggt tttggcgata tgcagcgcgt atgcctcgcc gttcaagcca
     6481 tattttaccg cctgccaaac ggttttgaag gacggtttct tgtcgtcgcc cgactttttc
     6541 gcttcggcac gcaatttggc aagcgcattt ttaaactctg ccgaccggtc gagtttcaga
     6601 cgtttcactt cctgtgcgac cacggtgttg accacttcgt tttccagcag ggattggcgc
     6661 agttgcggcg tgtcttcggc acggctgttt tccgcacgga atgcggcaac ctgcgcatcg
     6721 atgacggaac tgtcgatttt ctgaccgttg acggttgcca gcgtttgggc aaacaggctg
     6781 ccggaacagg caagcagtgc aacggaagtc aggatttttg ctttcataat cttctctctt
     6841 taaattttaa attgaaatgt tcgggcagac ttcattcagt ccgccgtatg gaaatactcg
     6901 tcaggggtag ccgcccggat gccgagcgcg tgaatcatgc cgcctgaaaa caaatctttg
     6961 agcagcgatt tgaccgtttt ctggcggttc aggcggctta cgccttcaaa acggccgctg
     7021 acgaccaaaa cggcataatg tccgccgccg gtattgcccg cgtgtccttt gtgcagatgg
     7081 ctctcatcgc cgatttccaa caccagcgga tcgagcgtct gcaggcgttc gcggatcaaa
     7141 tcgacggccg gcattatttg aacaccgctt taaacggctt gatcagcact tcgctgtaca
     7201 cgcctgcatg aacataggga tcgtcttccg cccacgcctc tgccgcatcc aaagactcga
     7261 actgcgccac aatcaaactg cccgaaacgc ggttggagtc ctccggcaaa ggattcgggc
     7321 ctgccgtcaa cagccggcct tccgacttca gcgtctccag ccgtttgagg tgttcgggac
     7381 gtgccgccat acgcgcttca tacacattct ccccgtctgt tgccagcaac ataaaatatt
     7441 ccacagtcaa tcctcctttt tcagacaggt actcagataa ataccctgaa taataaagaa
     7501 aacaagcatc agtgcagtcg agccgaacat tttatagttg acccattgcg cttcaaacct
     7561 agtaaacaca aaccagttgg caatacccat aaaaatcaga aaaccgaccc acatatacgt
     7621 caatttcgcc catacggcat ctgaaagctg aatctccctg ccgatactcg ccttcaagcc
     7681 gtttttaccg gcgaggtggc tgccccacag gaacagcgcg cccagccaaa acaaaacgct
     7741 cggcttccac ataatgaagc ggctgtcgcc caaaacaatg gttgcgccgc cgaataccac
     7801 aatcagcacc aatccgaccc actgcatcgt atccagcttt ttatatttcc aatacagaaa
     7861 agccgcctga accacaccgg caaccaatgc gaccgccgtt gcggcaatca tgtttttggt
     7921 aacggtatag gtggcgaaaa acaggatgac ggacaaaagg tcgctgacga atttcatatc
     7981 caaatgagaa agcaaatcaa ataaacgcta atcatacagg cagaaccctg tttttgcacc
     8041 tgcaaaacca acaaattttg ttttgattga tgcaaatatg cacagatgtt tttgaaaaaa
     8101 gatggagata tgtcataatt tgtaaaaacg gcgaatgatt gccgtttaaa atgtggcggc
     8161 ggtcggtaca ttcacatatt aaatgcccgc cggccgactg ccccgccgat gcccgatgat
     8221 gacgaaattt acagactgta cgcggtcaaa ccgtattcag ccgccaaccc acaggggata
     8281 catcttgaaa aacaacagac aaatcaaact gattgccgcc tccgtcgcag ttgccgcatc
     8341 ctttcaggca catgctggac tgggcggact gaatatccag tccaaccttg acgaaccctt
     8401 ttccggcagc attaccgtaa ccggcgaaga agccaaagcc ctgctaggcg gcggcagcgt
     8461 taccgtttcc gaaaaaggcc tgaccgccaa agtccacaag ttgggcgaca aagccgtcat
     8521 tgccgtttct tccgaacagg cagtccgcga tcccgtcctg gtgttccgca tcggcgcagg
     8581 cgcacaggta cgcgaataca ccgccatcct cgatcctgtc ggctactcgc ccaaaaccaa
     8641 atctgcactt tcagacggca agacacaccg caaaaccgct ccgacagcag agtcccaaga
     8701 aaatcaaaac gccaaagccc tccgcaaaac cgataaaaaa gacagcgcga acgcagccgt
     8761 caaaccggcg tacaacggca aaacccatac cgtccgcaaa ggcgaaacgg tcaaacagat
     8821 tgccgccgcc atccgcccga aacacctgac gctcgaacag gttgccgatg cgctgctgaa
     8881 ggcaaaccca aatgtttccg cacacggcag actgcgtgcg ggcagcgtgc ttcacattcc
     8941 gaatctgaac aggatcaaag cggaacaacc caaaccgcaa acggcgaaac ccaaagccga
     9001 aaccgcatcc atgccgtccg aaccgtccaa acaggcaacg gtagagaaac cggttgaaaa
     9061 acctgaagca aaagttgccg cgcccgaagc aaaagcggaa aaaccggccg ttcgacccga
     9121 acctgtaccc gctgcaaata ctgccgcatc ggaaaccgct gccgaatccg ccccccaaga
     9181 agccgccgct tctgccatcg acacgccgac cgacgaaacc ggtaacgccg tttccgaacc
     9241 tgtcgaacag gtttctgccg aagaagaaac cgaaagcgga ctgtttgacg gtctgttcgg
     9301 cggttcgtac accttgctgc ttgccggcgg aggcgcggca ttgatcgccc tgctgctgct
     9361 tttgcgcctt gcccaatcca aacgcgcgcg ccgtaccgaa gaatccgtcc ctgaggaaga
     9421 gcctgacctt gacgacgcgg cagacgacgg catagaaatc acctttgccg aagtcgaaac
     9481 tccggcaacg cccgaacccg ctccgaaaaa cgatgtaaac gacacacttg ccttagatgg
     9541 ggaatctgaa gaagagttat cggcaaaaca aacgttcgat gtcgaaaccg atacgccttc
     9601 caaccgcatc gacttggatt tcgacagcct ggcagccgcg caaaacggca ttttatccgg
     9661 cgcacttacg caggatgaag aaacccaaaa acgcgcggat gccgattgga acgccatcga
     9721 atccacagac agcgtgtacg agcccgagac cttcaacccg tacaaccctg tcgaaatcgt
     9781 catcgacacg cccgaaccgg aatctgtcgc ccaaactgcc gaaaacaaac cggaaaccgt
     9841 cgataccgat ttctccgaca acctgccctc aaacaaccat atcggcacag aagaaacagc
     9901 ttccgcaaaa cctgcctcac cctccggact ggcaggcttc ctgaaggctt cctcgcccga
     9961 aaccatcttg gaaaaaacag ttgccgaagt ccaaacaccg gaagagttgc acgatttcct
    10021 gaaagtgtac gaaaccgatg ccgtcgcgga aactgcgcct gaaacgcccg atttcaacgc
    10081 cgccgcagac gatttgtccg cattgcttca acctgccgaa gcaccgtccg ttgaggaaaa
    10141 tgtaacggaa accgttgccg aaacacccga tttcaacgcc accgcagacg atttgtccgc
    10201 attacttcaa ccttctgaag tacctgccgt tgaggaaaat gcagcggaaa tcgttgccga
    10261 tgatttgtcc gcactgttgc aacctgctga agcaccggcc gttgaggaaa atgtaacgaa
    10321 aaccgttgcc gaaatacctg atttcaacgc caccgcagac gatttgtccg cattacttca
    10381 accttctgaa gtacctgccg ttgaggaaaa tgcagcggaa accgttgccg atgatttgtc
    10441 cgcactgttg caacctgctg aagcaccggc cgttgaggaa aatgcagcgg aaatcacttt
    10501 ggaaacgcct gattccaaca cctctgaagc agacgctttg cccgacttcc tgaaagacgg
    10561 cgaggaggaa acggtagatt ggagcatcta cctctcggaa gaaaatatcc caaataatgc
    10621 agataccagt ttcccttcgg aatctgtagg ttctgacgcg ccttccgaag cgaaatacga
    10681 ccttgccgaa atgtatctcg aaatcggcga ccgcgatgcc gctgccgaga cagtgcagaa
    10741 attgctggaa gaagcggaag gcgacgtact caaacgtgcc caagcattgg cgcaggaatt
    10801 gggtatttga ttcccaactg ccctttcgca gatcaaggat gccgtttcag acggcatctt
    10861 tttttgcctt atcggtgtaa cggataaagt ttgaaccggc acaggctcaa acagcaggcc
    10921 gacggcaaca aaatgccgtc tgaaacccct aaaggcttca gacggcattg gcggcggcgg
    10981 tatcaggcag tttgataggc aaccgaatcg tcaccgcttt tcttttgaat gaactcgatt
    11041 ttgtatccgt cggggtcttc gacgaaggct atcacggttg tgccgtgttt catcgggccg
    11101 gcttcgcgga cgacgtttcc gccctgccgc ttcacacgtt cgcaggcttc gtaggcatcg
    11161 tccacttcaa ccgcgatgtg tccgtaggcg ttgcccaagt cgtatcgttc cgtatcccag
    11221 ttgtgcgtca gttccaaaac cgtgctgtcg gtttcgtcgc cgtaaccgac gaaggcaagg
    11281 gtaaatctgc cttcgggata atcttttcgg cggagcagtt tcatacccaa aacgttttgg
    11341 tagaaatcga gggatttttc gagattgccc acgcggagca tagtatggag taagcgcatt
    11401 tttgtgttcc tctcggtagt ggttaaactt cgattttgtt tggcgtaaac gtctgccatt
    11461 tgttgcaggc aggacaatgc cagaaaaaga cttgtgattt gaagtggcag tttcggcacc
    11521 ggtacatcac gctgcgctgt agctgccgtc cgataaccga acgcatcata tcggcatcgg
    11581 ctttccaagc cggatccaaa tcgctgagtt tcaaaccaag caggcggtac acgccgttga
    11641 ggtcgggctt gcggcggaca agctcgacgg cggtttgcgc ggcttctttc tcgcacttaa
    11701 gcagcaggga tttctcgtac acgacattga tcaggtcaag ttcgggaaac gtctgcatat
    11761 atcctgtcag acggttcaag ccttcttcag gttttccctg cgcggcatag gcttcgtaaa
    11821 gcttctcgcc gaccatgctc aagtatgcat ggttttgctg ctcgatggcg gcataggctt
    11881 cgacggcggc agggaaattg ccttgtcggt gttcgatgtc gcccaaaatc atgttggcac
    11941 gggtgcattt tttgttggct tcgagtgcct tgccgacatt gaaacgcgcg acatcgaaat
    12001 tggacttgaa cagcgcggct tgggcaagtt cgcaataaaa ctgggcgatt tcaaactgat
    12061 aggtctgatc gtcatggctg agcagccggg cggtttcaac cgctttttcc caatccctgt
    12121 cctgttggta gatattgagc aggtgctgtc tggcttcacg cgccatttta ccgtcttgca
    12181 gccccaaaaa aatctgttcg gcacgatcga ccaaccccgc actttggtag ttttgcgcca
    12241 attcaaacag gacgcgcgcg cgcttttcgc cgaccgtatc gggagaatcg agcattgtcc
    12301 ggtgtatgtt gatggctttg tcgttttcgc cacgctgacg gtaaagcttg cctagggtga
    12361 ggtttaggtc gtatgactga ggctgctgat caatgacctc tgccaattcc ctggcggcac
    12421 gtccgctgtt gcggtcaacc aaggcatcca gacttttata aaatcccgac ggtatgcttt
    12481 ttgcctgctt taatacagtc ttcatatcca cacgggcggc aaaccagccc atcgcgaaga
    12541 aaacgggcaa aaggataatc ggcagcagga taatccacaa ttcattgtcc atatcggctt
    12601 tctcaaggct gtttggcaga ttcgggcgca ttttgcgccg gtggtgcggt cagctctttc
    12661 cccgtcaaac gcgcattttt ctttacttcg gcacgcaacc tgccgttctc gccacgtaac
    12721 gacaacaacc gtccgaacaa ggcaaacatt ccgaacacga tgccgacgac aaacgcgccg
    12781 aacaatacga caatcagcgg caaatcgact ttctgtccgg gcaggtagga gaaggtaacg
    12841 gcatccgtat taatgacggc aagcagcagg aagaacagca ggataatgat tttgacgacg
    12901 gtatagataa gtttcatgtt tgctccgtaa ataaaaacgc cgcccggaca gcttcgcact
    12961 ctgaaccaag attgcgctag tttaaacgat ttgcacggtt tcggatagga tgcggcagcg
    13021 tgttcggacg acatacggag tatgcgattg ccgacaattt taccaaatac accgttcctt
    13081 tccatttgaa aaataacgga ttggacaccg catcgacaga aaaacccgcc gcgcacttgt
    13141 caaaaccctg tttgcaggcg tatctttaca atcttcaaat tcaaaccgtt cattgaaaca
    13201 tatcagaata agaaaggctt tacatcatga gcagacccgt acccgccgta ttcggcagcg
    13261 tttttcacag tcaaatgccc gtcctcgcct accgcgaagg caaatggcag ccgaccgaat
    13321 ggcaatcttc ccaagacctc tccctcgcac cgggtgcgca cgccctgcac tacggcagcg
    13381 aatgtttcga gggactgaaa gccttccgtc aggcagacgg caaaatcgta ctgttccgtc
    13441 cgactgccaa catcgcgcgt atgcggcaaa gtgcggacat tttgcacctg ccgcgccccg
    13501 aaacccaagc ctatctcgac gcgctaatcg aattggtcaa acgcgccgcc gatgaaattc
    13561 ccgatgcgcc tgccgccctg tacctgcgtc cgaccttaat cggtaccgat cccgttatcg
    13621 gcaaggccgg ttctccttcc gaaaccgccc tgctgtatat tttggcttcc cccgtcggcg
    13681 actatttcaa agtcggatcg cccgtcaaaa ttttggtgga aaccgaacac atccgctgcg
    13741 ccccgcatat gggccgcgtc aaatgcggcg gcaactacgc ttccgccatg cactgggtgc
    13801 tgaaggcgaa agccgaatac ggcgcaaacc aagtcctctt ctgcccgaac ggcgacgtgc
    13861 aggaaaccgg cgcgtccaac tttatcctga ttaacggcga tgaaatcatt accaaaccgc
    13921 tgaccgacga atttttgcac ggcgtaaccc gcgattccgt actgacggtt gccaaagatt
    13981 tgggctatac cgtcagcgaa cgcaatttca cggttgacga actcaaagct gcggtggaaa
    14041 acggtgcgga ggcgattctg accggtacgg cagccgtcat ctcgcccgtt acttccttcg
    14101 tcatcggcgg caaagaaatc gaagtgaaaa gccaagaacg cggctatgcc atccgtaagg
    14161 cgattaccga catccagtat ggtttggcgg aagacaaata cggctggctg gttgaagtgt
    14221 gctgatgctt taaataaaaa atgccgtctg aaacccgttt ggcgtttcag acggcatttt
    14281 cgcatccgaa ccgtttccgc tgcacctgca gcaagtcggc acaaaggcaa tcggttaaaa
    14341 caagcgtccg catttctcat cccgcctgcc gcaagtcggg tatttctctg gaaatacgct
    14401 tcagacggca aaacgctgcc cgaaaccgat acgcggcacg gacggcgcac agatttgaaa
    14461 acagcggatt atccctcggt gctcaaggca ttgatgctgt aaccgccgtc aacgtaagta
    14521 atttcgccgg taataccgga cgacaggtcg gacagcagga aggcggcggt attgccgact
    14581 tcttcgatgg taacgttgcg gcggagggga ttgtgggcgg cgacgtgtcc caagagtttg
    14641 ccgaaatcgg cgatgccgga ggcggcaagc gttttgatcg ggccggcgga aatgccgttg
    14701 cagcggatgc cttctttgcc caaacaggcg gcggtaaagc ggatgcctgc ctcaaggctg
    14761 gcttttgcca tacccatcac gttgtagttc ggaatcgcgc gcaccgcgcc caagtagctc
    14821 agggcgacga tggcggagtt tctgccgcgc atcatcggac gggcggcttt tgccagcgca
    14881 ggcaggctgt atgcggaaat ttcgtgtgcg gtgttgaacg cttcgcggct gatgctgtcg
    14941 aggaaatcgc cgctcaaggc ttctttcggc gcgaagccga tggaatgcac caaaccgtcc
    15001 aagccgtccc aatgcttgcc caagtcggcg aacacttggt tgatttcgtc atcgctggcg
    15061 acatcgcagc ggaatacgag ttcggaatcc aattccgccg ccattttgcg gacgcgctct
    15121 tccagtttgt ccacaacgta ggtaaacgcc agttccgcgc cttgttcgcg gcaggctttg
    15181 gcgatgccgt aagcgatgga acgctcggaa atcatgccgg taatcagaat ttttttgcct
    15241 tgcagaaaac ccattttgta tccttcaaat agtgttcggt tcgttctaaa ccgttgatta
    15301 tagcaaattg tccctgtttc tgtgttttca cgttgcagcg tgcaaacggc aatgccgtcc
    15361 gaagcggatt tcagacggca tcggacgttt caaatacggt ttaaggcatc agatgccgcg
    15421 caacaattcg ttcacgctgg ttttcgcacg ggtttgcgcg tccacacgtt tgacgatgac
    15481 ggcgcagtaa aggctgtggc tgccgtcttt ggaaggcata ctgccggaca cgacgaccga
    15541 accggccggt acgcggcctt ggtagatttc gccggtggtg cggtcgaaga ttttggtgga
    15601 ttgaccgatg aacacgccca tcgaaatcac gctgccttct tcgacaatca cgccctcaac
    15661 gatttcagaa cgcgcaccga tgaagcagtt gtcttcaata atggtcgggc tggcttgcag
    15721 gggttcgagt acgccaccga tgccgacacc gccactcaag tgcacgtttt taccgatttg
    15781 cgcgcaagag ccgacagtcg cccaagtatc aaccatcgcg ccttcgtcga cgtatgcgcc
    15841 gatgttgacg taagacggca tcagcacgac atttttcgcc acaaagctgc cgcgtcgggc
    15901 aaccgcaccc ggaacggcgc ggaagcctgc gtttttgaac tcgtcttcag accagtcggc
    15961 aaacttggtc ggcactttgt cgaagtattt gttcacgccg tcgttgagga cttcgttgtc
    16021 ttggatgcgg aaggacagca acacggcttt tttcgcccat tcgttgactt tccactcgcc
    16081 cacgcccaag cgttcggcaa cgcgcagttt gcctgaatcg agttggcgga tggtttccaa
    16141 cacggcttct ttaacttcgg gagcaacagt ggacggggtg atgtccgcgc ggttttcaaa
    16201 ggcggtttcg ataatgtttt gcaaagacat aatgtttcct tatgtgagat gtttcgtggg
    16261 acgcttgccg gtattttcaa gcagtatgtt catttgaaat gaatgcgcta ttgtgccacg
    16321 caaacgcata ttcgtcagca atacgcgggc ggttgccgaa cattggcgtt tcggaatcgg
    16381 gcgggcggca ggtattgcct tttccgcacc cgaatcaata cagaacggcg gcggtacttt
    16441 tatgccgcgc cgccgccttt cagacggcat tcacggtaaa acgccatcag cccttcggtc
    16501 gaggcatcgt gtacggacgt gccgccttcc agttcgccga tgatggtttt cgccaactgt
    16561 ttgccgtatt ccacccccca ctgatcgaag ggattgatgt cccaaattac accctgcaca
    16621 aaggttttgt gttcgtaagc cgccatcagc atacccagat tgtagggcgt gaggcgttcg
    16681 agcaggatgc tgttgctggg gcggttgccg gggaactctt tgtgcggcgc gaggcgttcg
    16741 cgttccgctt cgggcaaatc tgccagttcg gcgcgtgctt cgtccaaggt tttgcccttc
    16801 atcagggctt ccgcttgggc aaaggcgttg gcaacggtaa aacggctgcg tccgtcctcc
    16861 acgccctgcg ccgtcatcgg gacgataaag tcgcagggaa tcaggcgcgt gccttgatga
    16921 agcagttgga aataggcgtg ctggcagttg accccttcac cgccgaacac gatgccgccc
    16981 gttttgcaca cggcgggact gccgtctgaa gcgcggcttt tgcccaaact ctccatatcg
    17041 agctggttca gccacaccgg cagcaggcgc aggttgtggc tgtacggaac ggcggtctgc
    17101 ccgtccgcgt gttggaaatt gttataccac acggcaatca gtgccatcag aacgggaatg
    17161 ttgcggcgcg gcggcgtgtg gaaaaaatgg ctgtccatcg cgtgcgcccc cgccaacaac
    17221 tcgcggaaac gcgccccgcc gaccgcaacc atcacgggca aaccgacggg cgaccagacg
    17281 gaatagcgtc cgcccaccca gtcgtacatc gcaaacacgc gttccgccgc gataccaaaa
    17341 gccgcagctg ccgcagtgtc ggcagacacc gcgcaaaaat ggcacgccgt ttcggattcc
    17401 gagaaccccg caccgcgata ccacgccttg actgcctgtg cattgagcag ggtttccggt
    17461 gttttgaagg acttgctggc aacgcaaaac actgtcgttt cggggttcag acggcataaa
    17521 accgcatcca ggcaggcagg atcggcgttg gcggcaaaat ggacggcgat atgccgtctg
    17581 aacggctcaa gtgcctgcac gcacattgcc ggcccgaggt cggatccgcc tatgccgatg
    17641 tggacaaaat ccgtaatccg ttttccggtt gtcccctgat acgaaccgtc gtccaaactg
    17701 tgtgcaaact tcaacgcacg atttaactcg cggcggattt cgggcaacac gtccctgccg
    17761 tccgcataaa cggcacccgc accgtcgggc aggcgcaaag ccgtatgcag cgcggcacgc
    17821 ccctcactgt tgttgacctt cgcacccgtc cgcaaagcac gcattttccc ttccaaatcc
    17881 gccgcatcgg caagattgca gagcagttgc agcgtatctt cgcccaaacg gtttttgctg
    17941 taatcgaaca acatcccgtc caaacgctcg tgcatacgct caaaacggtc gggttcggca
    18001 gcaaaacggt cgcgcaaaag aatgtggcgc gtatcctgat aatggcgttc gagcgcacac
    18061 catgcacggg taaaagcatc catctgtttt ccttgatttt tcagaaccgg attaaaatgt
    18121 agtagaatgt agtttaacaa acggcagcgg ctttggcgaa tctccggaac accgcccgcc
    18181 gcgtccgata gcaccttatt tccatatggc gaaaatatag tgaattaaca aaaatcagga
    18241 caaggcggcg agccgcaaac agtacaaata gtacggaacc gattcacttg gtgcttcagc
    18301 accttagaga atcgttctct cttgagctaa ggcgagccaa cgctgtaccg gtttttgtta
    18361 attcactata ccctccggcc ggcatttgac ccgcaacaat atcctgcaag atttattgtg
    18421 tacgcataaa tgccggacag ccgcctaaat aaggtataat cgcatccgat tctgtcccgt
    18481 ttgccgcccg caggcgggat gtcggcggtt ttcatcttac cgggaacacg ccggtactgt
    18541 tttttaacga tgtttcttac attttattcc aattacttta cggggctaga atatggctaa
    18601 aaacggagga ttttctttgt tcgcaaagaa agaaaaacgc tttatctttg aaggcaggca
    18661 ttccgcctcc gacaaactgg tcaacggcga agtatccgcg tttaccgaag aagaggcgcg
    18721 caaaaaactg gcaaaacgcg gcatccgccc gttgcagatt acccgtgtga aaacaagctc
    18781 caagcgcaaa atcacacaag aagacatcac cgttttcacc cgccagctgt ccacgatgat
    18841 taaagcgggc ctgccgctga tgcaggcatt tgaaatcgtg gcgcgcggac acggcaaccc
    18901 gtctatgacg gaaatgctga tggaaatccg aggcgaagtg gaacagggca gctcgttgag
    18961 ccgcgcattc tcaaaccacc caaaatattt cgaccgcttc tactgcaatc tggttgcggc
    19021 gggcgaaacg ggcggcgtat tggaaagcct gctggacaaa ttggcaattt acaaagaaaa
    19081 aacccaggcc atccgcaaaa aggtaaaaac cgcactgacc tatccggtat ccgtcatcgc
    19141 cgtcgccatc ggtttggtat tcgtgatgat gattttcgta ctgcccgcct ttaaagaagt
    19201 ttacgccaat atgggcgcgg agcttcccgc actgacccaa acagtgatgg atatgtccga
    19261 ctttttcgtc tcatacggct ggatggtgct gatcgcactg ggctttgcca tatacggctt
    19321 ccttaaattg aaggcgcgtt cgattaaaat ccaacggcgt atggatgcca tactgctgcg
    19381 tatgccgatt ttcggagaca ttgtccgcaa agggacgatt gcccgctggg gcaggacgac
    19441 ggcgacgctg attgcggcag gcgtgccttt ggtcgatgta ttggactcca ctgccggcgc
    19501 ggcgggcaat ttaatctatg aagaagccac ccgggaaatc cgtacgcgcg tcatccaagg
    19561 tttgtctatg acttcgggga tgcgtgcgac ggaactgttc cccaatatga tgttgcagat
    19621 gtcttccatc ggcgaggaat cgggttcttt ggacgatatg ctcaacaaag ccgccgaatt
    19681 ttacgaagac gaggtggaca atgcggtcgg caggctgtcc gctatgatgg agccgatcat
    19741 tatcgtgatt ttaggcttgg tcatcggcac gcttctggtc gccatgtatc tgccgctgtt
    19801 taatttgggc aacgtggtcg cctgatttgc cgcacagatc cggcgcggat tcgctctgcg
    19861 ccggtttgtt tttgttttga atacattgag aacaaaatat gtctgatttg tctgtattgt
    19921 cgccgtttgc cgtgcctttg acagcggtgt tcgggctgct ggtcggaagt ttcttaaatg
    19981 tcgtcattta ccgcgtgccg gtcatgatgg aacgcggctg gacggtattt gccaaagaat
    20041 atttaaacct gccgctgacc gaagaggaaa gccgtacctt caacctgatg aaaccggatt
    20101 cctgctgtcc caaatgccgc gtgccgatac gcgcgtggca gaacatcccg attgtcagct
    20161 acctgctcct gcgcggcaaa tgcgcctcct gccaaaccaa aatcagcata cgctatccct
    20221 taatcgaact gctgaccggc gtattgttcg ggctggttgc ctggcaatac ggctggtctt
    20281 ggattacatt gggcggtttg atactgaccg cgtttctgat ttccctgacc tttatcgatg
    20341 cggacaccca atacctgccc gactcgatga cattaccctt gatttggctg gggctgatat
    20401 ttaatttgga cggcggcttc gtgcctttgc agtctgccgt tttaggtgcg gttgccggct
    20461 atggttcgat atggctttta tgcgcggtat ataaactgat taaaggcgaa atcggtatgg
    20521 gcaacggaga tttcaaactg attgccgcat tgggcgcgtg gctcggcata tccgcattgc
    20581 ccgtgctgat ttttgtttcc tcgctgatcg gtttggtcgc ggcaatcgtt atgcgcgtcg
    20641 ccaaggggca gcattttgcc ttcggccccg cactgacagt ttcgggctgg ataattttta
    20701 cggcaaacga ttccgtatgg cgggcggtca actggtggct gacccatccg gtgctgtaag
    20761 atgacggtat gggtcggact gaccggcgga atcggcagcg gcaaatcggc agccgcgcaa
    20821 tgttttgccg atttgggcgt accgcgtatc gatgcggatg cggcggcgca ctcgctgacg
    20881 gcttcagacg gcatcgccct gccggaaatc aggcggctgt tcggcgacac cgttttcgac
    20941 acacagggtt tgttgcgacg cgacatattg cgtaaagaag tctttgcctc cccatcgcga
    21001 aaagccttgc tcgaatccgt gatgttgccg ctgattttct cagaaatcaa aaaacagcaa
    21061 gaaaccttta ccgatgcggt ttacggcatt gtcgaaattc cgctgctgac ggaaaagcgt
    21121 caatttatca gcctgatacg gcgtgtcctg accataagtg cccctgtgga aaaacgtatc
    21181 ggcagggtga tggcccgcag cgggctgacg cgcggcgagg tggcggccgt catcagccat
    21241 caggcatccg aatccgaacg cctgctgctt gcagacgatg tgctgctcaa tgacggcagc
    21301 ctcaaaagcc tgcgtgagaa aacaatgcgc ctgcacgcgt tttattcagg gattttcgcc
    21361 tcaaaaccaa cacaaggaaa acacaatgac tgaatcgcgg caaacacgcc ttcaagtcaa
    21421 atgtccgacc tgtcaaacgg cagtagtatg gaaaccggaa aacgcattcc gccccttctg
    21481 ttcgcaacgc tgcaaactga tcgacttggg cggatgggca gacgggaaat atacggtttc
    21541 cagccaaacg gaaagtttgc cggaaatatc cgaacccgac atggcatacc gctgaccgcc
    21601 ccgccttccc ggcaaacacc ctgaaagtca aatgccgtct gaaacaaaca cgcttcagac
    21661 ggcattttca ttctcaaacc taatcgttgg tatttgccgt tacctcttcc aatgaagtaa
    21721 tgccctgcat aactttcaaa ataccggccc ggcgcaaatc caccataccc tccttatagg
    21781 caacgtccaa aatatccact tccgtaccgt tgttcataat cacacgctgc atttcttcgc
    21841 tgatgggcat aacctcatac acgcccgcac gccctttata accctgcccc cggcaacggt
    21901 cgcaaccgac ggcgcggtaa agtttccaat cttttgcaag gtcctcatcg gtgaagccga
    21961 cttccttcaa agcagaggca gacgggcgtt ccacttcctg tttgcagctc gaacacagcc
    22021 tgcgtaaaag acgctgcgcc ataatcaggc tgaccgaact ggcaatatta aacggcgcga
    22081 cacccatatt cagcatacgc gacaacgtcg ccggcgcatt attggtgtgc agggtggaaa
    22141 acaccatatg ccctgtttgt gccgccttaa tcgcaatatc ggcagtttcc aaatcacgaa
    22201 tctcaccgac cataatgatg tccgggtcct gacgcaggaa agacttcaaa gcagcggcaa
    22261 aagtcaggcc ctgcttatca ttgacgttaa cctgattgat gcccggcagg ttaatctcgg
    22321 cagggtcttc cgccgttgca atatttaccg actccgtatt caaaatattc aaacaggtat
    22381 agagcgacac cgtcttaccc gaacccgtcg gaccggttac cagcaccatc ccgtagggac
    22441 ggtgaatcgc ttccaacaac aattttttct gaaacggctc aaaaccgagc tggtcgatgt
    22501 tcaaagacgc ggcatcggaa ttcaaaatcc gcatcacgac cttttcgcca aacagcgtcg
    22561 gcaatgtgct gacacggaaa tcgacaggct tgccgccctt ttgaaaggtc agctgcatcc
    22621 tgccgtcctg cggtatccgt ttttcggaaa tgtccaaacg cgacattacc ttaatccggg
    22681 aagcaagctg cccccttacc gcaatgggcg gctgaaccac ctcgcggagc tgcccgtcca
    22741 cacggaaacg gatacgggca ttgtgttcgt aaaactcgaa atggatgtcc gatgccccgc
    22801 tgcgcaaggc atccgacaaa gtcttatgga taaacctcgg aacagggccg tcttctgcct
    22861 cctcgttgtc gatatacagg gtgtggcttt cctcttcctc ctgcccctcc ccaagctcct
    22921 gaagcagcga tgtcgaacgc gaacccaccc aatcgagcaa acccgccaac tggtcatcct
    22981 cgacaatgac caattcaacc tcaatccctg cggcagaaac ggttttctga atttgcggca
    23041 tctgtgtcgg atcggaaacc gcaaaaaata ctttgtcgcc ccgacggaaa accggcacac
    23101 agtggaactc caccatctgc tcctccgtca acacccccat cagcaccctg tggcgcggat
    23161 aatgacgcaa atcaagaatc gaataactga acaccctcgc aatcaatgcc gcaagcgact
    23221 tgggcgaaat gacaccgtct gaaaacagca tcggcaacac ttccttaccc gcctgcgact
    23281 cattgtagta atgctcggcc tgctcaacag taaccacctg gttttgaacc agaatcctca
    23341 gcaaacctac gctcatacga ccttatcccc aaatttattc attgttatac ctgtacagct
    23401 tttatagtgg attaaattta aaccagtacg gcgttgcctc gccttagctc aaagagaacg
    23461 attctctaag gtgctgaagc accaagtgaa tcggttccgt actatttgta ctgtctgcgg
    23521 cttcgtcgcc ttgtcctgat ttaaatttaa tccactatat cataaaacaa cagatttcca
    23581 agccggaaca tcttttacgg agcctgaaaa tcatttcatg attctaccgt cctaaaggtc
    23641 ggtttttcaa gcaggaagaa aaagttttca gatggcaaaa aagccctcca acactgaaag
    23701 gccttatatc ggaaacttcc cgcaacacgg gaaacagaca aatgcaaatc gtcaaacctc
    23761 gccaacagga atcgaacctg tattttacgc ttaggaggca tacgttctat ccgttgaact
    23821 atggcgagcc gaaatgaaaa ggagatttta accctttccg acgacaaaag acaagatgcc
    23881 ggccgcaatc agcggcccga cagggatacc gcccataaat gccaccccga taaccgtccc
    23941 gattaacagc cctgtaatta aaacaggctg ctgtcccatc aaaggcacgc cgcgtcccgc
    24001 cagccaagcc acgaaaatac cgataaaaac ggcggatatc attttaaaat tcaaaaattc
    24061 ggcaacggga ggaacctgcg cctttcctga aaccaacgga ctcaaaaccc ctatggtcaa
    24121 aagaatgata ccgagattca acccatgctt ctcgaccaac gggacaaact gtaccaatgc
    24181 cgtctgctgc atcagcagca atatggttgc cgagatggta atcgaattgt tgttgctgac
    24241 cacccccaac agaatcagcg taaccagaaa caggggaaca aaactgaaat tcatcgcctc
    24301 aagacaccct gaccaaagat tccttcgcca gctgaatcat ggcatccttc acttcgctgt
    24361 cgggcagggc atccaaggcg gtaacggcac aatcgactgc tttgcgcgcc tcgcctatcg
    24421 aatatgccaa agcatccgaa cggacgacat aatcgtggat tttctcaaaa tagctgcgat
    24481 ctgcattttc caaagcagta cgcacatcgt tcgcaacctg ttcggaaccc tgacgcatca
    24541 gataaatcaa aggcaaagtc ggttttcctt ccgccaaatc gtcgccgacg tttttgccgg
    24601 tttcttcggt ttcgccagaa tagtccagca catcgtcaat aatttggaat gccgtaccga
    24661 catacatacc gtagtctttc aacgcccgtt cgtgttcggg ggaagccttg cccaaaattg
    24721 cgccgacttg agcggcagct tcaaacaatt ttgccgtttt atattggatg acttggatat
    24781 attgttcttc ggtaatgtcc gtattgccga tgttcatcag ctgcatgact tcgccctcgg
    24841 caataatgtt ggttgcatcc gccatcactt ccaaaacgcg catactgccc gaggcaacca
    24901 tcagttgaaa ggcgcgcgtg tataaaaagt cgccaaccaa cacagccgcc gcattgccga
    24961 acagattgtt tgccgttgcc cgcccacggc gcaaatcgct ttcatcgacg acatcgtcgt
    25021 gcaggagggt ggaagtgtgg ataaactcga ccatcgccgc cagcgaatac agtttctcgt
    25081 catcataacc gaccgcctta cccgccaaaa tcgtcataat cggacgcagg cgtttgccgc
    25141 ccgcgctgat gatatatgta ccgatttgcg aaatcagtgc gacatcggat tgcaccgcac
    25201 ggttgatgac ttcattgact ttggcaaggt cttcaggcag atgtcgctgg aaatagggca
    25261 gattctcgag catggcagac tctcaggttt acgtttccgt caaacggcag ataccgcttg
    25321 aaagctgatt caaatagcgg gcaattatat cagcaaagcc atcagggtac atcttttcag
    25381 acggcatatg gttatccgaa ccgcgcaaac tttgacaaaa caggcaaata acaatacaat
    25441 gccgagttcc gcacaatggt gtgcggatat taaccataac ccttatggag ttgagtatgt
    25501 acgcggtcgt aaaaaccggc ggcaaacagt ataaagtttc cgtcggcgaa aaattgaaag
    25561 tagaacagat accagccgaa ctcgacagcc aaatcgaact gaccgaagtt ttgatgattg
    25621 ctgacggcga atctgtaaaa gttggcgcac cctttatcga aggtgcaaaa gtaacggcta
    25681 aagtagtggc acacggtcgt ggcgaaaaag tccgcatctt caaaatgcgc cgccgcaaac
    25741 actaccaaaa acgccaaggc caccgccaaa atttcaccca aatcgaaatc gtggcaatcg
    25801 cctaacctta aaacttagga gcattacaaa tggcaagtaa aaaagcaggc ggcagcaccc
    25861 gcaacggtcg cgattcagaa gccaaacgct tgggcgttaa agcctacggc aacgagctga
    25921 ttcccgcagg ttccatcatc gtacgccaac gcggtaccaa attccacgca ggcgacaacg
    25981 taggtatggg caaagaccac actttgttcg ccaaagttga cggttatgtc gaattcaaaa
    26041 ccaaaggcgc gctgaaccgt aaaactgtca gcatccgtcc ttacaccggt tctgaagaat
    26101 aatccaaccg atacctgaag ccgcatcttt tcacgatgcg gctttttata gtggattaac
    26161 aaaaatcagg acaaggcgac gaagccgcag acagtacaaa tagtacggca aggcgaggca
    26221 acgccgtact ggtttaaatt taatccacta tacatacccc acacccgatt tgatacacat
    26281 taagctgaaa gtaaaaatcc gcatacaccc tcccctgcat atttcttcaa cagtgggttt
    26341 tgatataatc gcctatctgt tacagatagt tcaaacggca ttcaaaccct tacaaatacc
    26401 gtctgaaatc atcgacatcc gtctatgcaa aggatatttt atgagtttac acagcgacat
    26461 cctcgtcgtc ggcgcaggcc cggcgggttt aagttttgca gccgaacttg ccggcagcgg
    26521 tttgaaagtt accctgatcg aatgcagccc actgactgtt ctgcaaaatc ctccatatga
    26581 cggacgcgaa atcgccctga cccatttttc ccgcgaaatc atgcagcgtt tgggtatgtg
    26641 ggacaaaatt cccgaaaacg aaatctaccc tttgcgcgat gccaaagtgc tgaacggacg
    26701 ttccgactac cagctccact ttccccaacc gaccgaggcg cgcggcgagc ctgccgactg
    26761 tttgggctat ctgatttcca accacaatat ccgccgcgct gcctacgaag tcgtatccca
    26821 actcgacaac attaccattc tgaccgatac ggccgttaaa gaagtcaaaa catctgacaa
    26881 cgaagcacaa gtcattttag aaaacggcaa aatcctgacc gcccgcctgc ttttggcggc
    26941 agacagccgt ttttcgcaaa cccgccgaca actcggcatt tcttcagata tgcacgacta
    27001 cagccgaacc atgttcgtct gccgcatgaa acatactctt tccaaccaac ataccgctta
    27061 cgaatgcttc cattacgggc gcaccatcgc actgctgccg ctggaagaac acctgaccaa
    27121 taccgtcatt accgtcgata ccgataaaat caacagcgtt caaaaccttt cgcccgaaga
    27181 attggcggca agcgtaaaag aacagctcaa gggcaggctc ggcgatatgg aattggtcag
    27241 cagcatccac cattaccccc ttgtcggtat gattgccaaa cgtttctacg gcaaacgcag
    27301 cgcgctgatc ggcgatgcgg ctgtcggtat gcaccccgtt accgcacacg gtttcaactt
    27361 gggtctgtcc agcgcagata ttttggcaaa actgatactc gaagccgagc agcgcggtca
    27421 ggacatcggc gcggcttcct tgttggaaaa atacagcagc aaacatatgc tgcatgccca
    27481 gccgctttat cacggaacca atatgatgct gaaactgttt accaatgaga ccgcgccggc
    27541 aaaactgctg cgcggtttgg tattgcgtgc cggcaacaac ttcccgccgc tgaaaaaact
    27601 gattaccaaa caactgaccg gttgaaaact gccgaaatgc cgtctgaaac gtttcagacg
    27661 gcatttttgt attatagtgg attaacaaaa atcaggacaa ggcgacgaag ctgcagacag
    27721 tacaaatagt acggaaccga ttcacttggt gcttcagcac cttagagaat cgttctctat
    27781 ccttggcaga cggtttgcag cagattcgca tacggcagat gtttcaagcg gaaaggaaac
    27841 acgataaaaa taacaaagcc tccgagcagt cggaggcttt gctttcaaac tgaaattatt
    27901 tcagtttagt ttctttatac actacgtgtt tgcgggcaac tgggtcaaat tttttgattt
    27961 ccaatttgcc gggcatagtg cgtttgtttt tggtagtggt gtagaagtga ccagtacctg
    28021 cactggattc cagtttgatt ttatcgcgca ttgcagtaat ccttaattaa atagtgttta
    28081 aattaagctt cgccgcgagc acgcaaatca gccaatacga catcaatgcc tactttgtcg
    28141 atggtacgca gtgcagcgtt ggaaacgcgc aggcgaaccc agcggttttc actttctacc
    28201 caaaaacgac gtgattgcaa gttgggcaaa aaacggcgtt tggttttgtt gttggcgtgc
    28261 gatacgttgt tgccggacat cgggcgtttg ccggtcactt tgcaaactcg tgccataatc
    28321 agtctccaaa cttctaaaat agtaaaacgc gatttatacc acgaaaaaca gtgttagttc
    28381 aagtgtttta aagaaaaacg gctgttttgc gcggataatc cttccgattg tcgcatctgt
    28441 ggaacgtttg caggtgcgaa acagatgggg cgcgattata gcggatttcg ggaactgctg
    28501 cattcaaaat gccgtccgaa catatttcca gtattttcag acggcatttc aagttttcaa
    28561 atccctcaat gtccaccgcc gccgccgttg tggaacggcg gttttgccag ccatatgacg
    28621 ggtatcatga taatgaataa caagctgccc gccataaaga tttcgttcga gccgataata
    28681 aaaccctgct gggtaatggt attgttgatg atgcctaggg tttgaatgtc ggaaacgccg
    28741 tgctgggaca aatgagcggc cgtttcgtgc aatgttgcgg aatagggcgt gatgtgttcg
    28801 gcaaagcgcg tgtggtgcaa cgcttcgcgc cgttcccaca gggtgctgac gacggatacg
    28861 ccgacaccgc ccatcagcac gcgcaggaaa ttcgacaggc tgccggcggc ggcaatctgc
    28921 ccgcccttca tgtgcgacag tgtgatggta gtcagcggca ggaagaacat ggcaacgcct
    28981 actccctgcc aaaactgcgg ccagatgacg ttgccgatat ccatgtcggc ataaaaatcg
    29041 gtgcgccaat agaaagtgaa ggcaaaggtc aggaagctgg cggttacgag caggcgcata
    29101 tcgattttat tgccgaacct gccgattaac ggagacagga agacgggcag gatgccgacg
    29161 ggcgcggcgg caagccccgc ccacgtggag gtatagccca ggttggtctg caacactaac
    29221 ggcagcaggg tcagcgtccc catatacacc ataaaaccca atgatgtggc aatgatgccg
    29281 acggtaaaat tccgatcttt aaacagcgat aaatcgacaa tcggatattt ttctcccaat
    29341 tcccaaacaa taaaatacga caagcacacc agtgcgacta cgcccaaggt aatgatttct
    29401 ccagaggcga accagtcgag ttccttaccc ctgtccaaca tcatctgtaa cgcgccgata
    29461 ccgactacca tcaatgtaag cccgacatag tcggtcggca ttttaacggt ttccgtttcc
    29521 cgacatttca aatgtttcca tgtaatccat gccgatatga taccgatagg gatattaatg
    29581 aagaaaatcc aaccccaatg ccagtttccg gaaatccagc cgccgagtat cggcccgaga
    29641 acaggggcga caacgacggt cattgcccac aatgccagtg ccagcgtccg ttttgcgggc
    29701 ggataggatg ccattaacag gctttgcgac aagggaatca gcggcccggc gataaagccc
    29761 tgcaagatgc ggaaaacaac cagcgactga aggttggggg caataccgca cagccacgat
    29821 gtgatgacga aaccgacagc ggcggcggta aacaatttga cctcgccgat acgttttgcc
    29881 aaaaagcccg ttaacggcac ggaaacggcg tttgccacag caaaagaagt aatgacccac
    29941 gtcccctgcg tggttgccgc accgaggttg ccggcgatga cgggaacggc gacattggcg
    30001 atagtcgtat ctaaaacttc cataaatacg gcaagcccca aagacagcgt aacccacgcc
    30061 aatgccgcac ccttaagcgg tggataatcc attgtgtcct ccgtttcaga cggcatcagg
    30121 gcgcggattg cccgaggatt tcatcgaccg tccgatcgac ttccgaccag tcggtacttt
    30181 ccatttccgg caatgccgca cccggcgttt ttgaaacagg cgcgcctgcg gcggaagtat
    30241 ccactttaac cgtcatcgac aaaccgatac gcaacggatg cctgtccaca tcttcgcggt
    30301 tcagcacgat acggacgggg acgcgctgca ccactttaat ccagttgccc gttgcgtttt
    30361 gcgccggaat cagcgaaaac gcgctgcccg tacctgccga aaaacctgcc accctgccgc
    30421 gataaacaat ttgtttgccg tacaaatcgg acaccagctc ggcaggctgt ccgattttca
    30481 tatgccgcaa ctgcgtctct ttaaaattag catccaccca cacatccgac agcggcacca
    30541 ccgccatcag cggcgcgcct gccgccacct gctgcccgac ctgcaccgaa cgcttcgcca
    30601 cctgaccgtc cgccggcgcg cggatttgcg tccgctgaag gttcaaccac gcatctttca
    30661 acctgccgat tgccgtctga accgccggct gttcgcgcaa agaaacctga ccgcccaaag
    30721 ccgcacgtgc cgaagattct tccgccaaag ccgctttgac cgccgcctgc gcctgagaca
    30781 ctgccgcacg ggcgtgtgcc agctcttcgg cggacaccgc gcccgattcc gccaaagcag
    30841 accggcggcg caaatcatcc tgtgcgcgtg ccaaatccgc ccggcgcaag gcaacctgcg
    30901 cccccgcctg cgaagtggcg gcattttgcc ggcggttttg ccgcaccgcc tgaaccagct
    30961 cgttttttgc ccgctcgtaa gccagcacat cattatcgtc gtccaatacc gccagcacgt
    31021 cgcctttttt cacggcatcc gtatcgtcgt gcaaaacctt ccgcaccgta ccgccctttt
    31081 gcggcgtaac ctgaaccacg cgtccggcaa cataagcgtc ttccgtttcc tcttcgtgct
    31141 gccaccataa aaaaaacgcc gacccggcgg ctgcggcggc aagcgcgaac agcagcgtca
    31201 atgccgtcag gcggcgtttg cgtttggctt gcgtgttttg aagttttgtt tcgtccgtgt
    31261 gcgtatccat aagattgggt tccgaatttc ctgatttcat ataaacaatg ttctttcaat
    31321 aggaagcaga ttctatatcc gcgcaataac gcaatcaagt cagtatttta gatgcaatat
    31381 tcaaaaaaca acgcttcagg cggcaaaact gaaaatatat ttcgtttata ta
//
LOCUS       DAWFLT010000018        30863 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWFLT010000018 DAWFLT010000000
VERSION     DAWFLT010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30863
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.018"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            80..1045
                     /locus_tag="WGR74_000809"
     CDS             80..1045
                     /locus_tag="WGR74_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0905261.1"
                     /translation="MLRLTALAVCTALALGACSPQNSDSAPQAKEQAVSAAQTEGASL
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSIDKNRLPYLDEYFKSTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKKAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGLPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            1209..2177
                     /locus_tag="WGR74_000810"
     CDS             1209..2177
                     /locus_tag="WGR74_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0905262.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMMLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSGVLLGRYELLWATGILALFAYLIADRLTILGLGETVSVNLG
                     LNRTAILWSGLIIVALITSLVIVTVGNIPFIGLVVPNIISRLIGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            2167..3141
                     /locus_tag="WGR74_000811"
     CDS             2167..3141
                     /locus_tag="WGR74_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ0905263.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCILFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELVVMMGGSLLLFYTLIRQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAAVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            3186..3812
                     /locus_tag="WGR74_000812"
     CDS             3186..3812
                     /locus_tag="WGR74_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905264.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAQDGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGTRDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLALRRRDTGKTVAVSLNAALQPFAPQ
                     MRDIMPKAVSGSASADELKQFGELWQERVRAFLIDQADNPEFVTVSEI"
     gene            3893..4651
                     /locus_tag="WGR74_000813"
     CDS             3893..4651
                     /locus_tag="WGR74_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0905265.1"
                     /translation="MITIRNVSHTIGSNPILNDVSLDIPEGGITALIGPNGAGKSTLL
                     SFMARLQPLVHGNISYAGKDIKTTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTREHKRTTVAVLHDINQAAAYADHVVAMKNGKAVLTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
     gene            complement(4710..5048)
                     /locus_tag="WGR74_000814"
     CDS             complement(4710..5048)
                     /locus_tag="WGR74_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ0905266.1"
                     /translation="MKKIEAVIKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            5143..5355
                     /locus_tag="WGR74_000815"
     CDS             5143..5355
                     /locus_tag="WGR74_000815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905267.1"
                     /translation="MPETVIPAQAGIPTSAFQQYLKVSVNPNFHIPDRAETTAFDPKT
                     YGSSCPLLCFLQLSAYPALGKNQKNR"
     gene            5513..9475
                     /gene="purL"
                     /locus_tag="WGR74_000816"
     CDS             5513..9475
                     /gene="purL"
                     /locus_tag="WGR74_000816"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ0905268.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFVG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDRQKQQWAALLHDRMTESVLTDIDAAAQLFHHIQSET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDAFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFSNNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHADDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVQDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGNAPD
                     LDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNGRSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRVHLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFTDPNTLTLGVCNGCQMVSNLAEIIPGTAGWPKFKRNLSEQFEARLSMVHVPKS
                     ASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQITQTYPLNP
                     NGSPQGIAGVTNADGRVTIMMPHPERVYRAAQMSWKPEDWTELSGWYRLFAGARKALG
                     "
     gene            9570..10322
                     /gene="gloB"
                     /locus_tag="WGR74_000817"
     CDS             9570..10322
                     /gene="gloB"
                     /locus_tag="WGR74_000817"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ0905269.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAERTPTLPVTLAHERRVNPFLRTEIP
                     AVRQRAEALVGKTLNSGLEVFAALRELKNAYC"
     gene            10556..12010
                     /gene="mgtE"
                     /locus_tag="WGR74_000818"
     CDS             10556..12010
                     /gene="mgtE"
                     /locus_tag="WGR74_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ0905270.1"
                     /translation="MSIEPTPPNLENDGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLGELHPADVAAVLESLPPRERNVVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRVTVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMVAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(12096..13004)
                     /gene="hslO"
                     /locus_tag="WGR74_000819"
     CDS             complement(12096..13004)
                     /gene="hslO"
                     /locus_tag="WGR74_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ0905271.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGRGRLKMLVAEAASDRTVRATARWDET
                     AEIADDESLGDLLGEGGVFVLTLQPKDGEPWQGVVPLEGGGIAQMLANYMKRSEQIDT
                     HIVLSASDEAAGGLLVQRLPEAVLDEEAWEHVSTLARTLTADELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            13249..14004
                     /locus_tag="WGR74_000820"
     CDS             13249..14004
                     /locus_tag="WGR74_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0905272.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            14023..14241
                     /locus_tag="WGR74_000821"
     CDS             14023..14241
                     /locus_tag="WGR74_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905273.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            14386..14730
                     /locus_tag="WGR74_000822"
     CDS             14386..14730
                     /locus_tag="WGR74_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ0905274.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            14730..15422
                     /locus_tag="WGR74_000823"
     CDS             14730..15422
                     /locus_tag="WGR74_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ0905275.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            15489..16709
                     /gene="argJ"
                     /locus_tag="WGR74_000824"
     CDS             15489..16709
                     /gene="argJ"
                     /locus_tag="WGR74_000824"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ0905276.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            16769..18178
                     /locus_tag="WGR74_000825"
     CDS             16769..18178
                     /locus_tag="WGR74_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ0905277.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGVPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            18368..19759
                     /gene="hrpA"
                     /locus_tag="WGR74_000826"
     CDS             18368..19759
                     /gene="hrpA"
                     /locus_tag="WGR74_000826"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ0905278.1"
                     /translation="MSPNFAQTLSKDRHFLQSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKTVAEHQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEENFNNRPEFTDPEIARSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            19860..20987
                     /locus_tag="WGR74_000827"
     CDS             19860..20987
                     /locus_tag="WGR74_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905279.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQCPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQGTQVLRIDGTKIVYIK"
     gene            21164..22741
                     /gene="lpt3"
                     /locus_tag="WGR74_000828"
     CDS             21164..22741
                     /gene="lpt3"
                     /locus_tag="WGR74_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HEZ0905280.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FMIAALYLFARYKASRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEITEVGS
                     AGASMLDKLWLPALWGVLEVMLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTQLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYIVPL
                     VLYSPNKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLSIRDGKAEYVYPQ"
     gene            22807..26106
                     /locus_tag="WGR74_000829"
     CDS             22807..26106
                     /locus_tag="WGR74_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase"
                     /protein_id="HEZ0905281.1"
                     /translation="MQNMKNQVRGSGMDARSNPANVSDGLQNSSGHIGTNTRYRLTKL
                     GEQMARLPIDPKIARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHER
                     FTDKQSDFLAYLNIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMCEWRELHHQLAQT
                     AIEMGLTTKETAFRRPPEIRQLTSSENAGDQDLSAKLKQKQLDKKQHRAQIRAAKEAG
                     YEQIHRALLTGLIANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVET
                     TKLYARDVAVIQPEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRP
                     VPYGKVAPEEAREIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDD
                     EALFAFYNERLPEMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSR
                     QNENHGNTVGWVENPTSAATAKTVGFDNPTYATQQPTPSPAREGRGEGKTVAAQTNFS
                     ATAANPLPQEREQSAAASAVSNDLHPANPQQTAPSPVGEGREEGKTVASQTNFSATTA
                     NPLPNPLPQEGEQSAAASAVSNDPQPQKQPAPQKDRLKPLPLADIRTFQAWLKTAERE
                     NPRLLFLSRDDLMQHAAAHITEEQFPKFWQTADGKFKLSYRFEPHHPLDGVTLTLPLT
                     VLNRLHAPSLEWLVPGMLREKIQLLIKALPKQIRRICVPVPDFITKFLESNPDRQAVI
                     IPQLAHFIAKSAGDMRIFEQIDQDAWAAQELPEHCYLNLLIIDDGGQELAGGRKLHEL
                     QQQLGQAAAVTFRDNTQEFERDNVTTWDIGTLPESIKFARGKQQLTGYLGLQKEKDGR
                     IALRLCDTTEAAEQAHRQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLR
                     DDLTQAVCDRAFIGEDELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAAYAELN
                     GKLGKHPLTHLLRLRLQTLLAAGFATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDA
                     AREADIQELEQMWQEKNDGLVKQGLPVSDDLTAFKWMIEELRVSLFAQELKTPYPVSV
                     KRLLKEWEDLN"
     gene            complement(26129..26551)
                     /locus_tag="WGR74_000830"
     CDS             complement(26129..26551)
                     /locus_tag="WGR74_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEZ0905282.1"
                     /translation="MTDIFTTSKRSFVMSKIHSKETKPEVLVRKFLFFQGFRYRKNDK
                     RYVGKPDIVLSKYKTVVFIHGCFWYGHSCNKGHIPKSNTDFWLEKITKNCERDIKNET
                     ELEKIGFKVIVVWECELKNKAICRERLNRLVREIKDAV"
     gene            complement(26555..28201)
                     /locus_tag="WGR74_000831"
     CDS             complement(26555..28201)
                     /locus_tag="WGR74_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HEZ0905283.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIGRNRREEKQASPCGRIPTGKKGLG
                     KLALFGLGNKIEISTIQGNERVTFTLDYAEIRRSKGIYQPEFRKESVESNIESGTTIT
                     LTELTKKQGYPLDNYVEHLSRLFDFPAQDFKIKVSLNGSEPKIIDGNLKYDLVTPQFE
                     WEYQDLATNISSLSSKFEQYEYSGLIQGKFITTEKPLKNNMKGITLFANGRMVNMPEF
                     FTDSESSHFYSYLTGWLNVDFIDNDDEDLIATDRTSLDWKHSKTSKLRVFLGEIVRAI
                     GQDWRKRRKKEKDDEVKGESGVDIEDWKNKLPEKEREPVEVILNRLEDSELTNKEQAE
                     VISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFEDKVQKQTG
                     LHSIDGRELIEQAFGSKNSILLLTNNKTKAEQNLEDGLEQLACGTWTGFRNPVQHELR
                     ANLSPSIFNDKDALDLISLVSYLLRKVEQTKKRSKVVSSK"
     gene            complement(28215..29270)
                     /locus_tag="WGR74_000832"
     CDS             complement(28215..29270)
                     /locus_tag="WGR74_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0905284.1"
                     /translation="MKNSKLKAVDFFCGGGGMSYGMQSAGIQVLAGIDYEPSCKETYE
                     ANINAKFIQADVFELQPETLEKELGLKKNDDDLILIGCSPCQYWSVIQTDKRKSEKSK
                     SLLLEFQRFVEYFNPGYVVVENVPGILSRMKESRLDNFIKLLEEKGFTVHFGIHNTAD
                     YGIPQSRKRFTLIANRITKEKLEPVKYSGKRLTVRDVLGMENGFPKIIAGHQDETDFM
                     HSCAGLSDINLKRLALIPKNGGNRLAFAHIPELQLECFIGKDNSFKDTFGRLWWDKPA
                     PTITTKFFSISNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIGNAVP
                     PMYAEKIGRAIVDNIEC"
     gene            29560..29772
                     /locus_tag="WGR74_000833"
     CDS             29560..29772
                     /locus_tag="WGR74_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905285.1"
                     /translation="MPRIHPPHLFPPHIAERGLLYFQQGKVRDVRKTSAGRYRAEVCG
                     LENYWVWLKLDSDLYIKDESCDCPYV"
     gene            30082..30237
                     /locus_tag="WGR74_000834"
     CDS             30082..30237
                     /locus_tag="WGR74_000834"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ0905286.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(30327..30776)
                     /locus_tag="WGR74_000835"
     CDS             complement(30327..30776)
                     /locus_tag="WGR74_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0905287.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINTVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         7558 a         9001 c         7320 g         6984 t
ORIGIN      
        1 tatcatttat cctttctaaa gccgttccgg tttgtccgac cggcggcttt gccccaatat
       61 ccccattttg gagacaccta tgttacgttt gactgcttta gccgtatgca ccgccctcgc
      121 tttgggcgcg tgttcgccgc aaaattccga ctctgcccca caagccaaag aacaggcggt
      181 ttccgccgca caaaccgaag gcgcgtccct gaccgtcaaa acggcgcgcg gcgatgttca
      241 aataccgcaa aaccccgaac gtatcgccgt ttacgatttg ggtatgctcg acaccttgag
      301 caaactgggc gtgaaaaccg gtttgtccat cgataaaaac cgcctgccct acttggacga
      361 atatttcaaa tccacgaaac ctgccggcac gctgtttgag ccggattacg aaacgctcaa
      421 cgcttacaaa ccgcagctca tcatcatcgg cagccgcgca gccaaagcgt ttgacaaatt
      481 gaacgaaatc gcgccgacca tcgaaatgac cgccgatacc gccaacctca aagaaagtgc
      541 caaagagcgt atcgacgcgc tggcgcaaat cttcggcaaa aaggcggaag ccgacaagct
      601 gaaggcggaa atcgacgcgt cttttgaagc cgcgaaaact gccgcgcaag gcaaaggcaa
      661 aggtttggtg attttggtca acggcggcaa aatgtccgcc ttcggtccgt cttcacgctt
      721 gggcggctgg ctgcacaaag acatcggttt gcccgctgtt gacgaagcca tcaaagaagg
      781 cagccacggt cagcctatca gctttgaata cctgaaagag aaaaatcccg actggctgtt
      841 tgtcctcgac cgaagcgcgg ccatcggcga agagggtcag gcggcgaaag acgtgttgaa
      901 taatccgctg gttgccgaaa caaccgcttg gaaaaaagga caggtcgtgt acctcgttcc
      961 tgaaacttat ttggcagccg gtggcgcgca agagctgctg aatgcaagca aacaggttgc
     1021 cgacgctttt aacgcggcaa aataatgaaa cggcggcatt cgatgccgtc tgaaacacgg
     1081 atgcaaaccg cctcctgtgt ttcagacggc attgcccgat acggaggctt caaacaaggc
     1141 tttccgctcc gacggttcgg actgccttgt ttgaatcttc tacgccttaa cgcttttccc
     1201 ttctgtttat gactgccaaa cctttttccc tcaacctgac caacctgctg ctgctggcgg
     1261 tgttgtttgc cgtcagcctg tcggtgggcg ttgccgattt ccgctggtct gatgtgtttt
     1321 cactgtccga cagccagcag gtcatgttca tcagccgcct gccgcgcacg tttgcgattg
     1381 tgctgacggg cgcgtcgatg gcggtggcgg ggatgattat gcagattttg atgcgtaacc
     1441 gttttgtcga gccttctatg gcgggcgcgg gtcagagtgc ggctttgggt ttgcttctga
     1501 tgtccctgct gctgcctgcc gcgccgctgc cggtcaaaat gtcggttgcc gccgttgccg
     1561 cgttaatcgg gatgttggtg tttatgatgc ttatccgccg cctgccgccg acggcgcaac
     1621 tgatggtgcc tttggtcggg attattttcg gcggcgtggt tgaggcggtg gccaccttta
     1681 ttgcgtatga aaacgaaatg ctgcaaatgc tgggcgtgtg gcaacagggc gatttttccg
     1741 gcgtgttgct cggacggtat gaactgttgt gggcaacggg gattttggct ttgtttgcct
     1801 atttgattgc cgaccggctg acgattttgg gtttgggcga aacggtaagc gtgaacttgg
     1861 ggctgaaccg gacggcgatt ctgtggtcgg ggctgattat tgtggctttg attacgtcgc
     1921 tggtcatcgt tacggtcggc aatattccgt ttatcgggct ggtcgtgccg aacatcatca
     1981 gccgcctgat aggcgacagg ctgcgccaaa gcctgcctgc ggtggctttg ttgggcgcgt
     2041 ctttggtgtt gctgtgcgac attatcggac gcgtgattgt gtttccgttt gaaattccgg
     2101 tctctacggt ttttggtgtg ttgggtacgg ctttgttttt gtggcttttg ttaaggaaac
     2161 ctgctcatgc cgtctgaaaa aaatatcggt tttatggcag gaagcagccg tccgttgtgg
     2221 gttgcctttg cgctgttgct ggtttcctgc atcctgttta tgacgctcaa cgtcaaaggc
     2281 gattgggact ttgtactgca cctgcgtctg accaaacttg ccgcgctgct gatggtcgcc
     2341 tatgcggtcg gcgtttcgac ccagcttttt caaacgctga ccaacaatcc gattctcacg
     2401 ccctcgattt tgggtttcga ttcgctgtat gtgtttttgc agaccttgct ggtgtttacg
     2461 ttcggcggcg tgggctatgc ttccctgccg ttgacgggca aattcggctt tgaactggtc
     2521 gttatgatgg gcggctcgct gctgctgttt tacacgctca tccgtcaggg cggacgcgat
     2581 ttgccgcgta tgattttaat cggcgtgatt ttcgggattt tgttccgcag cctgtcgtcg
     2641 ctgctttcgc gcatgatcga tcccgaagaa tttaccgccg cgcaggcgaa tatgtttgcc
     2701 ggattcaata ccgtccacag cgagcttttg ggcataggcg cgctgattct gctcgtcagc
     2761 gcggcagcgg tttggcgcga acgctaccgc ttggacgtac accttttggg gcgcgaccaa
     2821 gccgtcaatt tgggcatcag ctacacgcgc aacaccttgt ggatactgct ttggattgcc
     2881 gcattggtgg cgacggcgac cgccgtggtc ggcccggtaa gctttttcgg gcttctcgcc
     2941 gcctcgcttg ccaaccactt ttccccgtcc gtgcgccatt ccgtccgcct gccgatgacg
     3001 gtttgtgtcg gcggcatcct cctggtcggc ggacagaccg tattcgaaca cttcttgggc
     3061 atgaaggcgg tattaagcgt ggtggtcgaa tttgcgggcg gactcgtttt cctctatctc
     3121 gttttaaaac acaaaaaatg acggatgcca tctgaacggc agcccgcccc gaaaggacaa
     3181 accatatgac gcaagaacgt ttacccgaat ttttcgaccg cgccccgacg ctgaccgtac
     3241 aagacccgct tgccgcattc ctcggcgcag cgcaagacgg catcctcact taccgctacg
     3301 ccgatgccgt gcgcctgtgc ggacattcct gcccgaccgt cgcgggcgcg tacctgatgg
     3361 ttatcaaagg tctgaaagcc ctttacggcg aagagctgcc cgaacgcggc ggcatcgaag
     3421 ccgccatgca gggaacgcgc gacgaaggca cggtcggcgt aaccgcgtcc gtcgtccaac
     3481 tcctcaccgg cgcagccccc gaaaccggct tcggaggcat cggaatgcag ggacgcttcg
     3541 cccgccgcaa cctcttatcc tttggtgcag gcgaaatcaa cggcacactc gcgctccgcc
     3601 gccgcgatac cggcaaaacc gtcgccgtca gcctcaacgc cgcgctccaa ccctttgcac
     3661 cgcaaatgcg cgacatcatg cctaaagccg tcagcggcag cgcaagtgcc gacgaactca
     3721 aacaattcgg cgagctttgg caagaacgcg tccgcgcctt cctgattgac caagccgaca
     3781 atcccgaatt tgtcaccgtc agcgaaatct aaaacgtcat aatcaataag tttcagacgg
     3841 ccctaaaact caaggccgtc tgaaacccca atttccattt tcggacccgc ctatgattac
     3901 catccgcaac gtcagccaca ccatcggcag caaccccatc ctcaacgacg tcagcctcga
     3961 cattcccgaa ggcggcatta ccgcgctgat cggcccgaac ggcgcaggca agtccaccct
     4021 cctctccttt atggcgcgcc tacagcctct ggtacacggc aacatcagct acgcaggcaa
     4081 agacatcaaa accaccccta ccgccgaact cgcccgcacg ctctccatcc tcacccaaga
     4141 aaacagcatc atgagccgca ttaccgtgcg cgacctgctg atgttcggcc gttaccccta
     4201 ccatcaaggc agaccgactg ccgaatgccg ccgtatcgtt aacggtgcaa tcgaagaatt
     4261 ccacctgcaa gacctctccg accgctatct gaccgagctc tccggcggcc aacgccaacg
     4321 cgccatgatt gcgatggtgt tctgccaaag caccgactac gtccttttgg acgaaccgct
     4381 gaacaacctc gatatgtatc acgcccgctc gctcatgcag attctgcaaa agctcacgcg
     4441 cgaacacaaa cgcaccaccg tcgccgtatt gcacgacatc aaccaggccg ccgcctacgc
     4501 cgaccacgtc gtcgccatga aaaacggcaa agccgtcctg accggcacgc ccgaagaagt
     4561 gttcaccgta cacaacatca aagaactgtt cgacatggac gtggacgtac tcgactacga
     4621 aggcaaaaaa ctcatcgtcc accatatctg aagctcaaaa agaaaatgcc gtctgaaagg
     4681 ctttcagacg gcatttccat tccaaacgct caaaccgccg cgtccgcacg ttcgcccgtg
     4741 cggatacgga ttgcctcctc gaccggcagc acaaaaatct tgccgtcgcc gatttttccc
     4801 gaacgcgcca cctcgacaat cacgtcaatc gcgcgttcca cagcatcatc cgccaacaca
     4861 agctcgattt tgaccttggg caggaaatcg acggcgtatt ccgcaccgcg atagatttcc
     4921 gtatgcccct tctgcctgcc gaaccctttg acctcgctga cggtcatgcc cgtaatgccg
     4981 atttccgtca acgcctcgcg cacgtcgtcg agtttgaacg gtttgataac agcttcgatt
     5041 tttttcatca cattctccat taattaacgt aaacaaacga caaatatagc agaaaccgcc
     5101 ggcaaacgat aaacggtact gttcgcccgg cgatgcggcg atttgccgga aaccgtcatt
     5161 cccgcgcagg cgggaatccc gacctctgca tttcagcaat atttaaaggt ttctgtaaat
     5221 ccaaacttcc atattcccga ccgtgcggaa acgacggcat ttgatccaaa aacatacgga
     5281 agcagttgcc cgcttctctg cttcctgcaa ttatccgcat acccggcttt gggtaaaaat
     5341 cagaaaaaca gataaaaccc ttattcctta atatcttcag acggcacctg tattttcccc
     5401 aaccgtatgc cgtctgaaga ttgtcaacat tttaagccaa aatatccaag caatacaaac
     5461 ctgtccggcg tataatccct ctttttccca accgcatttg agagccgaat ccatgtctgt
     5521 cgttttgccc ttgcgcggcg ttaccgccct ttccgatttc cgtgtcgaaa aactcttgca
     5581 aaaagccgcc gcactcggtc tgcccgaagt caaattaagc agcgaatttt ggtatttcgt
     5641 cggcagcgag aaagcacttg atgccgcgac tgtcgaaaaa ctgcaagcct tgttggcggc
     5701 gcaaagcgtt gaacaaacgc caaaagcgcg cgagggcttg catttgtttt tggtcacgcc
     5761 ccgtttgggt acgatttcgc cgtgggcttc caaggcgacc aatatcgcgg aaaactgcgg
     5821 tttggcaggc attgagcgta tcgaacgcgg catggcggtg tggctggaag gtgcgcttac
     5881 cgacaggcaa aaacaacaat gggcagccct gttgcacgac cgcatgaccg aaagcgtgtt
     5941 gaccgacatc gatgcggcgg cgcaactgtt ccaccacatc caatcggaaa ctttctccac
     6001 cgttgatgtt ttgggcggcg gtaaagaagc tttggtcaaa gccaataccg aaatgggctt
     6061 ggcactctcc gccgacgaaa tcgattatct ggttgaaaac tatcaggctt tgcagcgcaa
     6121 tccgtccgat gttgaattga tgatgttcgc gcaggcaaac agcgaacact gccgccacaa
     6181 aatcttcaac gccgatttca tcctcaacgg cgaaaaacaa ccgaaatccc tcttcggtat
     6241 gatacgcgac acgcacaacg cgcatcccga aggcacggtc gtcgcttata aagacaactc
     6301 gtccgtaatc gaaggcgcga aaatcgagcg tttctatccg aatgcggcgg aaaaccaagg
     6361 ttaccgtttc cacgaagaag acacgcatat catcatgaaa gtggaaacgc acaaccaccc
     6421 gaccgccatc gcgccgtttg caggcgcggc aacaggcgcg ggcggcgaaa tccgcgacga
     6481 aggcgcgacg ggcaaaggtt cgcgtccgaa agcaggcttg accggcttta ccgtctccaa
     6541 cctcaacatc cccgacctca aacagccgtg ggaacaagac tacggcaagc cggaacacat
     6601 ttcctcgccg ttggacatca tgattgaagg ccccatcggc ggcgcggcgt tcaacaacga
     6661 attcggccgc cccaacctct tgggctactt ccgcactttt gaagagaagt ttgacggtca
     6721 ggttcgcggc taccacaaac cgattatgat tgccggcggc ttgggcagca ttcaggcgca
     6781 gcaaacacac aaagacgaaa tccccgaagg cgcgttgctg atccaactgg gcggcccggg
     6841 tatgcttatc ggcttgggcg gcggcgcggc ttcttcgatg gataccggta caaacgacgc
     6901 ttctttagac ttcaactccg tacaacgcgg caaccccgaa atcgaacgcc gcgcgcaaga
     6961 agtcatcgac cgctgctggc agcttggcga caaaaacccg attatttcca tccacgacgt
     7021 cggcgctggc ggcttgtcca acgccttccc cgaactggtc aacgatgccg gacgcggcgc
     7081 ggtattcaag ctgcgcgaag tgccgttgga agaacacggt ttaacgcctt tgcaaatctg
     7141 gtgcaacgaa tcgcaagagc gttatgtctt gtcgattttg gaaaaagatt tggatgcctt
     7201 ccgcgccatc tgcgaacgcg aacgctgccc gtttgccgtg gtcggcacgg cgaccgacga
     7261 cggtcatttg aaagtgcgcg acgatttgtt ctccaacaat cccgtcgatt tgccgctgaa
     7321 cgtcttgctc ggcaaaccgc ccaaaaccac gcgtaccgac aaaacggtta cgccgtccaa
     7381 aaaaccgttt cacgccgacg atatcgacat caccgaagcc gcctaccgcg ttttgcgcct
     7441 gcctgccgta gccgccaaaa acttcctgat taccatcggc gaccgcagcg tcggcggcat
     7501 gacccaccgc gaccaaatgg tcggcaaata ccaaaccccc gtagccgact gcgccgttac
     7561 catgatgggc ttcaatacct atcgcggcga agcaatgtct atgggcgaaa aaccgaccgt
     7621 tgccctgttt gacgcgcccg cctcgggcag aatgtgcgtc ggcgaagcca tcaccaacat
     7681 cgcggcggtc aacatcggcg acatcggcaa tatcaaactc tccgccaact ggatggcggc
     7741 gtgcggcaac gaaggcgaag acgaaaaact ctaccgcacc gtcgaagcgg tttccaaagc
     7801 ctgtcaggca ttggatttga gcattcccgt gggcaaagac agcctgtcga tgaaaaccgt
     7861 atggcaggat ggcgaagaga aaaaatccgt cgtttcgccg ttaagcctga ttatctccgc
     7921 gttcgcgccg gttcaagacg tacgcaaaac cgttacgccc gaattgaaaa acgtcgagga
     7981 cagcgtattg ctgtttgtcg atttgggctt cggcaaagcg cgtatgggcg gctctgcatt
     8041 cggtcaggta tacaacaaca tgagcggcaa cgcgcccgat ttggacgata caggccgtct
     8101 gaaagccttt tacaacgtga ttcaacagct tgtcgccgaa gacaaactct tggcgtatca
     8161 cgaccgcagc gacggcggtt tgtttgccgt tttggtagaa atggcgtttg cagggcggtg
     8221 cggcttggat atagatttaa atttattgct tgcacaaaca tttattacca accataccgc
     8281 tctgtctcaa tcattgcgga ctgaagaggt aaaagcgttg gctgaatggc aagaaaccat
     8341 tgcccgcaca ttatttaatg aagagttggg tgctgttatc caagttagaa aacaagatgt
     8401 tgccgatatt atcaatttat tctatcaaca acagctgcat cataatgtct ttgaaatcgg
     8461 tacgttaact gatgagaaca cgttaatcat ccgcgacggg caaacgcacc ttatttctga
     8521 caacctaatc aaactgcaac aaacctggca agaaaccagc catcaaatcc aacgcctgcg
     8581 cgacaaccct gcctgcgccg acagcgagtt tgccctgatt ggcgacaacg gacgcagcgc
     8641 attgtttgcc aacctgaaat tcgacgtgaa cgaagacatc gccgcgccgt ttatcaacag
     8701 cggcgcgaaa cccaaaatcg ccatcctgcg cgaacagggc gtaaacgggc aaatcgaaat
     8761 ggccgccgcc ttcacccgtg ccggtttcga tgcctacgac gtgcatatgt ccgacctgat
     8821 ggcaggccgc gtccaccttg ccgacttcaa aatgctggcg gcgtgcggcg gcttcagcta
     8881 cggcgacgta ctcggcgcgg gcgaaggctg ggcgaaatcc atcctgttcc accccgcctt
     8941 acgcgaccag ttcgccgcct tcttcaccga cccgaacacg ctgacattgg gcgtgtgcaa
     9001 cggctgccag atggtcagca acctcgccga aatcatcccc ggcacggcag gctggccgaa
     9061 gttcaagcgc aacctgagcg aacagttcga agcgcgcctg agcatggttc acgtccccaa
     9121 atcagcctcc ctgattctga acgaaatgca aggctccagc ctgcccgtcg tcgtcagcca
     9181 cggcgaaggc cgcgccgact tcgcgcttca cggcggcaac atttctgccg atttgggcat
     9241 tgcgttgcaa tatgtggacg gacaaaacca aattacccaa acctacccgc tcaaccccaa
     9301 cggctcgccg caaggcatcg ccggcgtgac caacgccgac ggccgcgtta ccatcatgat
     9361 gccgcatcct gaacgtgtat accgcgccgc acaaatgagc tggaaaccgg aagactggac
     9421 ggaattgtcc ggctggtacc gcctcttcgc cggcgcacgt aaagccttgg gctaaccgcc
     9481 ctactcaaac caatgccgcc tgaagaatat ttcagacggc gttccggcat accatccttt
     9541 aaacggtatc cgtccaccga ggaacactca tgaaaatcac acccgtcaaa gccttgaccg
     9601 acaactacat ctggatgata cagcacggca accatgccgt ctgcgtcgac ccttccgaac
     9661 cctcgcccgt cttggaattc ctcgtccgca accgcctcat gcttgcccaa acatgggtaa
     9721 cgcaccccca ccccgaccac gagggcggtg cggcggcact ctggcgcggc tacatggaat
     9781 cgcccgttta cggcgaatcc gacatcgaag cagcaaccca caccgtaacc gccggcaccc
     9841 aattcacctt cggcgacgga caggttaccg tttgggcaac acccggccac acagaccgcc
     9901 acaccagcta ccttctcgaa acttcagacg gcatacacgt cttttgcggc gacacccttt
     9961 tttccgccgg ctgcggacgc gtgtttaccg gcacaatcga acagctttac gacagcttcc
    10021 aacgcttcaa ccgcctgcct gaaaacaccc tgttctaccc ggcacacgaa tacaccgccg
    10081 ccaacctgcg tttcgccgcc catatcgagc cggacaacgc cgacattcag acggcattga
    10141 aagcggcaga acgcacgccc accctgcccg ttaccctcgc ccacgaacgc cgcgtcaacc
    10201 cgttcttacg gacagaaatc cccgccgtcc gccaacgtgc cgaagccctg gtcggcaaaa
    10261 cgctgaacag cggtttggaa gtattcgccg ccctgcgcga actgaaaaac gcctactgct
    10321 gacctgccct ccgaaaaatg ccgtctgaaa cccgcgtttc agacggcatt tgcgttaaaa
    10381 atagtaaacc gtttcaaaag ggagtagaat agtgccgttt ccaaccctgc gcctgtaccg
    10441 tcaggctttt attatggacc ttcccagttc gtttttactg aacacccctt ccgattccaa
    10501 acgacaataa ccatcccgcc ggaatgcctc cccgcgcacg gcgggcggag catttatgag
    10561 catcgaacca acccctccga accttgaaaa cgacggtatc gaaaacgatg tagaacgcgt
    10621 ttccgccgat ttcgaccgtg tccactccct ctgcgaaatc ctcgaacctg cttttgaaca
    10681 aatcgaaaac ggcacaccgc tcgaagacgc gccgctgcgc gacaagctga ccgagctgac
    10741 cgtcctcttg ggcgagctgc accctgccga cgtggcggcg gtgttggaat cgctgccgcc
    10801 gcgcgaacgc aacgtcgttt ggcttctggt caaacccgaa gacgacggcg aagtattgct
    10861 ggaagtatcc gacgcggtgc gcgaaacact gatcgagtcg atggataagg acgaattgtt
    10921 ggcggcagtt gacgatttgg acgcggacga attggcggaa ctggcagacg atttgccgca
    10981 ccaagtggtt tacgaagcct tacagacgcg tgatgaggaa gaacgcgccc aagtcaaggc
    11041 ggcaatgtcc tacgaagaca accaagtcgg tgcgattatg gacttcgagt tggtcagcat
    11101 ccgcgccgat gtcgcctgcg aagtggtgct gcgttacctg cgccgcttcg acagcctgcc
    11161 cgaccacacg gataaaatct tcgtggttga cgaaaacgat gtgttgcagg gcgtgctgcc
    11221 catccgcaaa cttttggtcg ccgatcccga agacttggtg gaaaacgtga tggcgaaaga
    11281 tgtcgtgcgt ttccgcgccg aagacgacgt ggaagaagca gcgcaggcgt ttgaacgcta
    11341 tgacttggtt accgcgcccg tcgtcgatga aaacaaaaag ctcatcggca gggtgaccgt
    11401 tgacgaaatg gtggacgtga tccgcgaaga atccgaagca gatatgttga acatggcggg
    11461 tttgcaagaa gaggaagact tattcgcccc cgtcttggat tccgtcaaaa accgctggat
    11521 gtggctcgcc gtcaacctct gcaccgcctt ccttgccagc cgcgtcatcg gcgcgtttga
    11581 aggcagcatc gaaaaaatcg tcgcactcgc cgcgctgatg cccatcgtcg ccggtatcgg
    11641 cggcaactcg ggcaaccaga cgattaccat gattgtccgc gccatggcga tggggcaact
    11701 gacggatatg caggcggggc gtttgctgaa aaaagaagtc ggcgtcgcct tggtcaacgg
    11761 catcatttgg ggaacggtca tgggcgcagt atcttggctg ctttacggca gcctcggcat
    11821 cggtttggtc atggttgccg cgatgacgct caacctgctg ctggcggcaa ccgtcggcgt
    11881 attaattccc gtggtaatgg aaaagttcgg acgcgatccc gcgctgggca gctcggtgct
    11941 gattacggcg gtaacggatt ccggcggctt cctgattttc ttgggtctcg ccaccctgtt
    12001 cctgctttaa atgccgtctg aacccgcgca aaaatgccgt ctgaagcgga agctgcttca
    12061 gacggcattt gactatttat ccttgttgca caagattatt ggacggtatg ccggggcagc
    12121 cctttggcaa cgccgaccac atcctccccg aacagcgcgt tgacatcggt ttcgtcaaac
    12181 acatatttgc tgtggcagaa atcgcaatcg acttcgatgc tgccttgttc caccaccacg
    12241 ccgccgactt cttccccgcc cagcatcagc agcatatcgc tgactttgcc gcgcgaacag
    12301 gtgcatgaaa attcaaacgt ttccggctcg aacacgcgcg gcggcgtttc gtggaacagg
    12361 cggtacaaaa catgttgcgc atccaattct gccagctcgt ccgccgtcag tgtacgcgcc
    12421 agcgtactga cgtgttccca tgcctcttca tccaacaccg cttcaggcag acgctgcacc
    12481 agcagaccgc ccgccgcttc gtcgcttgca gacaggacga tgtgcgtatc gatttgttcg
    12541 gaacgcttca tatagttcgc caacatttgc gcgataccgc cgccttccaa aggcactacg
    12601 ccctgccagg gttcgccgtc tttgggctgc agcgtcagca cgaatacgcc gccctcgccc
    12661 aaaaggtcgc cgaggctttc gtcatcggct atttctgcgg tttcgtccca acgcgcggtt
    12721 gcacggacgg tacggtcgga agccgcttcc gcaaccagca ttttcagccg cccccgcccc
    12781 tgaacctgca caatcagcgt gccttcgttt ttgaggttgc ccgacagcaa cacacccgcc
    12841 gccaacaact cacccaaagc gcggcggatg gcggcgggat agtttttctg ttttacaatg
    12901 tgctgccaca cgttttccag acggacgtgc agcccgcgca cgggcatatc gtcgaagata
    12961 aagcgggtac gcacatcggc gcggttgatg gcggtttgat tcatgatttt ctctgactga
    13021 ttgttcggat ggcggctata tggttgcggt cggcgcgaaa acaagacgga cggcggatgc
    13081 gcttcccaaa ttatcaataa attatataaa aatcaacata ttaactcaat ctaacaagcc
    13141 gttttttgcc aaacagccgt ttttttatat acaatcaaca agatattttc gactgataca
    13201 gcataacatc gcacggcggc acgatgcctc ctgcgcggaa acaccgatat ggattctttt
    13261 ttcaaaccgg cagtttgggc ggttttgtgg ctgatgtttg ccgtccgccc cgcccttgcc
    13321 gacgagttga ccaacctgct cagcagccgc gagcagattc tcagacagtt tgccgaagac
    13381 gaacagcccg ttttacccat caaccgagcc cccgcccggc gggcgggcaa tgccgacgaa
    13441 ctcatcggca gcgcgatggg gcttaacgaa cagcccgttt tacccgtcaa ccgagtcccc
    13501 gcccggcggg cgggcaatgc cgacgaactc atcggcaacg cgatggggct taacgaacag
    13561 cccgttttac ccgtcaaccg agcccccgcc cggcgggcgg gcaatgccga cgaactcatc
    13621 ggcaacgcga tgggactttt gggtattgcc taccgctacg gcggcacatc gatttctacc
    13681 ggttttgact gcagcggctt catgcagcac atcttcaaac gcgccatggg catcaacctg
    13741 ccgcgcacgt cggcagaaca ggcgcggatg ggtacgccgg ttgcccgaag cgaattgcag
    13801 cccggggata tggtgttttt ccgcacgctc ggcggcagcc gcatttccca tgtcggactt
    13861 tatatcggca acaaccgctt catccacgcg ccgcgcacgg ggaaaaatat cgaaatcacc
    13921 agcctgagcc acaaatattg gagcggcaaa tacgcgttcg cccgccgggt caagaaaaac
    13981 gacccgtccc gctttctgaa ctgatttccc aaggaatacg caatgagtat gcccgaaatg
    14041 cccaaatggt acgacgatga cggacagatc gtgtcctgta ccgaaaaggt caaagtgatg
    14101 tccgaaaata tggccgagct gtatcagacg gcacaagacg cgtttgaaga cgcgctgctg
    14161 atgggttgcg gcgaacgtca gttgcgcgat tacctgctcg cgctgattga aggtttggaa
    14221 aatccctacc gcaaagtctg aacacgcccc ggttgctgcg gcacggttta tccgtgccgt
    14281 ttttgcgttt gtgcgcggct tcggcttttc agacggcata tttgacgtta tgattaaaca
    14341 gttaacaaga tttatcacaa cgccgtcaaa agacagacac acaacatgaa catcatccgc
    14401 gcgctcctca tcatcctcgg ctgcctcgcc gccggcgaaa ccgccgtttt cctagcaggc
    14461 atcaaactgc ccggcagcat cgtcggcatg ggcgtgctgt ttgcgctttt gcaggcaggt
    14521 tgggtcaaaa cgtcttggct acaacagctt accgacgcgc tgatggcgaa tctgacgctg
    14581 tttctcgtgc cgccctgcgt ggcggtcatc agctatttgg atttgattgc cgacgattgg
    14641 ttttcgatac tggtttccgc ctccgccagc actttgtgcg tactgctggt tacaggcaag
    14701 gttcaccgct ggatacggag cattatctga tgaacgaaat cctcaggcag cccagcgttc
    14761 tgcttttcct cacgcttgcc gtgtacgcgc ttgcgattat cgtgcgcacg cgcacgggca
    14821 atatcttctg caaccccgta ctcgtcagca ctatcgtgct gattgcctac ctgaaaatcc
    14881 tcgacatcga ttatgcggtg taccacaacg ccgcgcaatt cattgatttt tggctgaaac
    14941 ccgccgtcgt cgtgcttgcc gtgccgctct accaaaaccg ccgtaaaatc ttcaaccagt
    15001 ggctgcccgt catcgtttca cagcttgcgg gcagcgttac gggcattgtt acggggatgt
    15061 attttgccaa atggctgggc gcggaacgcg aagtcgtcct ctcgctcgcg tccaaatctg
    15121 ttaccaaccc catcgccatt gaaatcaccc gctccatcgg cggcattccc gccattaccg
    15181 ccgccaccgt catcattgcc ggtctggtcg gacagattgc cggttacaaa atgctgaaga
    15241 acacagtcgt catgccctcg tccgtgggta tgtcgctcgg cacggcttcg cacgcgatgg
    15301 ggattgccgc ctcgctcgaa cgcagccgcc gcatggcggc atacgcggga ctggggctga
    15361 cgttcaacgg cgtactgacc gcgctgattg cgccgctgct catccccgtt ttgggatttt
    15421 gaacccgttt cagacggcat ttcagcccat gctgtctgaa cgccgacaca ctcgcaagga
    15481 gaaccgttat ggctgtcaac ctgaccgaaa aaaccgccga acaactgccc gacatcgacg
    15541 gcattgccct ctacaccgcc caagcaggcg tgaagaagcc cgggcatacc gacctgacac
    15601 tgattgccgt agccgccggc agcaccgtcg gtgcagtctt cacgaccaac cgtttctgtg
    15661 ccgcgcccgt ccacatcgcc aaatcgcacc ttttcgacga agacggcgtg cgcgccctcg
    15721 tcatcaacac gggcaacgcc aacgcgggta cgggcgcaca gggcagaatc gatgctttgg
    15781 cagtgtgtgc cgccgccgcc cggcaaatcg gctgcaaacc gaaccaggtg ctgcccttct
    15841 ccaccggcgt gattctcgaa ccgctgcccg cagacaaaat catcgccgcc ctgcccaaaa
    15901 tgcagcctgc cttctggaac gaagcggcac gcgccatcat gaccaccgac accgtgccca
    15961 aagccgcctc gcgcgaaggc aaggtcggcg acaaacacac cgtccgcgcc acgggcatcg
    16021 ccaaaggctc gggcatgatt catcccaata tggcgaccat gctcggtttc atcgccaccg
    16081 atgccaaagt ttcccaaccc gtcctccaac tgatgacgca ggaaatcgcc gacgaaacct
    16141 tcaacaccat caccgttgac ggcgacacca gcaccaacga cagcttcgtc atcatcgcca
    16201 ccggcaaaaa cagccaaagc gaaatcgaca acatcgccga cccgcgttac gcccaactca
    16261 aagaattgtt gtgcagcctc gcgctcgaac tcgcccaagc catcgtccgc gacggcgaag
    16321 gtgcgaccaa gttcatcacc gtccgcgtcg aaaacgccaa aacccgcgac gaagcccgcc
    16381 aagccgccta cgccgtggca cgttcgccgc tggtcaaaac cgcctttttc gcctccgacc
    16441 ccaacctcgg caggctgctc gccgccatcg gttatgccgg cgttgccgac ctcgataccg
    16501 acctcgtgga aatgtatctc gacgatattt tggttgccga acacggcgga cgcgccgcaa
    16561 gctacaccga agcacaaggg caggcggtaa tgtcgaaggc cgaaatcacc gtccgcatca
    16621 agctgcatcg cggacaagcc gccgccaccg tctatacctg cgacctgtcg cacggatacg
    16681 tttccatcaa cgccgactac cgttcctgac ccgacacggc ttcagacggc atacataaaa
    16741 ttccgtctga accgccggac aacataccat gacctccaca ttcccccgcc gcctcgcccg
    16801 caaaatccgc caaacccgcc gcctgtcgcg caaaagcatc gcctttctgt tccttttggc
    16861 aggttcggca ctcgtcgccc tgaccgcgct gttttttgcc catcttgccg attttgcgct
    16921 ggaactgaac gccaaactgg ttcaacaata cccatggttc gcgtgggtcg cgcttccttt
    16981 gggtttgccg cttattgcgt ggctcacacg caaattcgcc cccttcaccg ccggcagcgg
    17041 catcccgcag gtcatcgcct cactgtcgct gccctacggc gcacagaaaa cgcggctgat
    17101 ccgcctcggg cagacactgc tgaagattcc gctgaccttt ttgggtatgc tgttcggcgc
    17161 gtccatcgga cgcgaaggtc cgtccgtgca ggtcggcgcg gcagtgatgg gcgcgtgggg
    17221 cgcgtggtgc aagaaacacg gcttggcatt caaagggatg caggaaaacg atttgatggc
    17281 ggcgggcgcg gcgggcggtt tggcagccgc gttcaacgcg ccgctggcgg gcgtgatttt
    17341 cgccattgag gaactcgggc gcggcatcat gttgcgctgg gagaggcaaa ttcttttggg
    17401 cgtgctcgcc tccggtttca tacaggtcgc cattcagggc aacaacccgt atttttccgg
    17461 cttcaacggc ggcgtattgg aacatatctt tctgtgggtc gcactgtccg gcctggtttg
    17521 cggcgcggcg ggcgggctgt tcggacgttt gctctatcgc ggtgcggcgg cgtttgcacc
    17581 gcgcaagata cgcggcttca tccgcaaccg tccgctgctg ctggcggcac tgatggggct
    17641 gctgctcgcc ctgctcggca cgttctacca aggcaaaacc tacggcaccg gctaccacga
    17701 agccgcccaa gccctgcacg gcatctacga agcccccttc ggactcgccg ccgccaaatg
    17761 gctcgccacc gtattcagct attgggcagg cgttccgggc ggcattttca ctccctcgct
    17821 gaccataggc gcggttttgg gcgagcatat cgccgccatc gccgacatat cgcagggtgc
    17881 aaacatcatc gtcctcatct gcatggcggc atttctggcg ggcgcgacac aatccccgat
    17941 tacttccgcc gtcgtcgtca tggaaatgac gggcggacaa agcctgctgt tttggatgct
    18001 gattgcctgc attttcgcct cgcaggtttc gcgccagttt tcgccgcgtc cgttctacca
    18061 cgcatcggga atgcgcttcc gccagcgcgt gcttcaagaa accgccgccc aaaccggcaa
    18121 tgcgcccgca agaccgcaaa cagcaaacag caaaacggga atgccgtctg aaaattaaaa
    18181 cgcccccgat caaacgccgg cagccgcctt gatttgaata ccgttccgcc gccgcttgaa
    18241 atttcagcaa caatgccgtc tgaacgacag aatgcggttt tcagacggca tttccccatc
    18301 ccgatattgc ccgaacccaa ccaaagcgtt tgctataatt tcatttttac cgtacccgca
    18361 cacaaatatg tcccccaatt tcgcccaaac cctctccaaa gaccgccact tcctgcaatc
    18421 cgccttcaaa aatcccaaca aatacggcgg cttgtccaaa gtcgaagaaa aataccgaaa
    18481 atcgcacgaa atctttttga aacgtttggc agccttgccc aaacccgaat tcgacaacac
    18541 cctgcccgtt cacgaaaaac ttgacgaaat caaaaaaacc gttgccgaac atcaggtaac
    18601 gattatttgc ggcgaaaccg gttcgggcaa aaccacgcag ttgcccaaga tttgtttgga
    18661 actcgggcgc ggggcggcag gattgatcgg gcatacccag ccgcgccgtt tggcggcgcg
    18721 ttcggtggca gagcggattg ccgaagagct gaaatccgaa atcggcagcg cggtcggcta
    18781 taaagtacgc ttcaccgacc acacctcgcg cgatgcctgc gtcaagctga tgaccgacgg
    18841 catcctgctg gcggaaacgc agaccgaccg ttatctcgcc gcctacgaca cgattatcat
    18901 cgacgaagcg cacgagcgca gcctgaacat cgacttcctt ttgggctatt tgaaacaact
    18961 cctgccgcgc cgccccgatt tgaaagtcat catcacctcg gcaacgatag acgcagaacg
    19021 cttctcccga cacttcaacg gcgcgcccgt tttagaagtg agcggacgga cgtatcccgt
    19081 cgaaatcctc taccgaccgc tgaccggcaa agacgaagac gacgcagaag tggagttgac
    19141 cgacgcgatt gtcgatgcgg cggacgaatt agcgcgacac ggcgaaggcg atattttggt
    19201 attcctgccg ggcgagcgcg aaatccgcga agccgccgaa gccctgcgca aatccacgct
    19261 gcgccgcaac gacgaaatcc tgcccctgtt cgcacgcctg tcgcacgccg agcagcacaa
    19321 aatcttccac ccctcaggcg cgaaacgccg catcgtattg gcaaccaacg tcgccgaaac
    19381 ctcgcttacc gtgccgggca tcaaatacgt catcgacacc ggcctcgcgc gtgttaaacg
    19441 ctattccgca cgggcgaaag tggagcagct tcatatcgaa aaaatctccc aagccgccgc
    19501 ccgccaacga tccggccgct gcggccgcgt ctccgcaggc gtatgtatcc gattgttttc
    19561 agaagaaaat ttcaacaacc gccccgaatt taccgacccc gaaatcgccc gcagcaacct
    19621 cgccgccgtc atcctgcgca tggcatcgct gaaactcggc gacgtagcgg cattcccatt
    19681 tttagaaatg cccgattcga ggtatattaa tgatgggttt caggtgttgc tggaattagg
    19741 ggcagtggag gccgcctgaa aacaagtagg atatacactt ctacctgaca aagaccaaca
    19801 aacgataaaa ctacggcaca tctagatgcc caacctacaa cattgcacac ggtaaaaaca
    19861 tgaaacactt actcatcgat tttgaaaacg tccagccgca aaacttagac aaattactaa
    19921 ccgaaaatac ccatatttgg ctatttatag gtgtattaca caaaatgtta cctattagtc
    19981 tggtgcaatc cctactacgt ttcggcgaac gtgtccatct tgtccagtta caaaaaacag
    20041 ggaaaaacgc attggatttt tacctgtcct attacctcgg acaaattacc gccacagacc
    20101 ccaatgccca aatcggcata ctctcgcgtg atggaggata cgatgttctg gtcgaacata
    20161 ttttgaaaaa ccaccaggcg aagggtatcg tgcgcctagc caatatagat gaagtacaac
    20221 atcagaaaat tactaccgaa ccgccgtcag cattgctgga aaacactcct cagcctgaaa
    20281 ccaccctcaa accacagcaa ccattaactt cctatttcca agcagcccta actgcactgc
    20341 gccgccccga cgctttccgc ccctgccgcc tgcataacct gcgacaaaat ctgcgtaagc
    20401 atattttgag tgatttgttt aaagaaaaaa ccgatgaaga atgcgaaata accactgcta
    20461 acgttatcaa taaactcaaa gcacaaaact tcatcagcat tgatgaacag gaaaccgttt
    20521 cctaccatct cagtgataat gatttgttac aaagaatcca acgccatatt ttaagccaat
    20581 gtcccaaaac ctacgctgat tttcaagccg tcgtgcaaaa ccgagcagat gcacttcact
    20641 taacagtcgg taccaacgac attcaatcct ttgcgcgaca tttgcgcgac caaaacctga
    20701 tccgccaaaa caatgggaaa attgaatatg caccgtttac tgaacctaaa ccacagccaa
    20761 cacccaagca gcctaaaaaa accgcatggg aacctgatga aattatttgg aaaaaagtga
    20821 ttgccgcgtt atcgttaaag aaccgtccta ataaaaccaa aactttacgc aatacaatcc
    20881 aggcactcac aaaatccaat gcacaagaaa ctgacaaact gctacaacat ttacaaggta
    20941 cccaagtcct acgtattgac ggtactaaaa ttgtgtatat caagtaggat gggcatttat
    21001 gcctgaccta caaaaccaaa aacaacctaa cacccttaat caactccaca aacccttttc
    21061 agacaacctc gttttttgaa aaacaatctg taaacagata actgctgaag aataccgttg
    21121 ccgagcccca aaacctgcct gcaactttta ttgtgaactt cccattatga aaaaatccct
    21181 tttcgttctc tttctctatt catctctact taccgccagc gaaatcgcct atcgctttgt
    21241 attcggaatt gaaaccttac cggctgcaaa aatggcagaa acgtttgcgc tgacatttat
    21301 gattgctgcg ctgtatctgt ttgcgcgtta taaggcttcg cggctgctga ttgcggtgtt
    21361 tttcgcgttc agcattattg ccaacaatgt acattatgcg gtttatcaaa gttggatgac
    21421 gggcatcaat tattggctga tgctgaaaga gattaccgaa gtcggcagtg cgggcgcgtc
    21481 gatgttggat aagttgtggc tgcctgcgtt gtggggcgtg ttggaagtca tgttgttttg
    21541 cagccttgcc aagttccgcc gtaagacgca tttttctgcc gatatactgt ttgccttcct
    21601 aatgctgatg attttcgtgc gttcgttcga cacgaaacaa gagcacggta tttcgcccaa
    21661 accgacatac agccgcatca aagccaatta tttcagcttc ggttattttg tcggacgcgt
    21721 attgccgtat cagttatttg atttaagcaa aatccctgtg ttcaaacagc ctgctccaag
    21781 caaaatcggg cagggcagtg ttcaaaatat cgtcctgatt atgggcgaaa gcgaaagcgc
    21841 ggcgcatttg aaattgtttg gctacgggcg cgaaacttcg ccgtttttaa cccagctgtc
    21901 gcaagccgat tttaagccga ttgtgaaaca aagttattcc gcaggcttta tgacggcagt
    21961 atccctgccc agtttcttta acgtcatacc gcatgccaac ggcttggaac aaatcagcgg
    22021 cggcgatacc aatatgttcc gcctcgccaa agagcagggc tatgagacgt atttttacag
    22081 tgctcaggct gaaaaccaaa tggcaatttt gaacttaatc ggtaagaaat ggatagacca
    22141 tctgattcaa ccgacgcagc ttggctacgg caacggcgac aatatgcccg atgagaagct
    22201 gctgccgctg ttcgacaaaa tcaatttgca gcagggcagg cattttatcg tgttgcacca
    22261 acgcggttcg cacgccccat acggcgcatt gttgcagcct caagataaag tattcggcga
    22321 agccgatatt gtggataagt acgacaacac catccacaaa accgaccaaa tgattcaaac
    22381 agtattcgag cagctgcaaa agcagcctga cggcaactgg ctgtttgcct atacctccga
    22441 tcatggccag tatgttcgcc aagatatcta caatcaaggc acggtgcagc ccgacagcta
    22501 tattgtgccg ctggtgttgt acagcccgaa taaggccgtg caacaggctg ccaaccaggc
    22561 ttttgcacct tgcgagattg ccttccatca gcagctttca acgttcctga ttcacacgtt
    22621 gggctacgat atgccggttt caggttgtcg cgaaggctcg gtaacgggca acctgattac
    22681 gggtgatgca ggcagcttga gcattcgcga tggcaaggcg gaatatgttt atccgcaatg
    22741 agtggcgtaa aaaccaataa agacaaattt agatgatgtc ggggaagatg cccgaccgac
    22801 aagactatgc aaaatatgaa aaaccaagta cgcggatcag gcatggatgc ccgatccaac
    22861 ccggccaatg tttcagacgg cctgcaaaac agttcgggtc atatcggtac caacacgcgt
    22921 taccgcctga ccaaactcgg cgaacaaatg gcgcgcctgc ccatcgaccc gaaaatcgcg
    22981 cgcattttgt tggcggcgaa gaagcacgac tgcatggcgg aaatattggt gatcgcgtcc
    23041 gcgctgtcga ttcaagaccc gcgcgagcgg ccgctggaag cgcgtgatgc cgcggccaag
    23101 gcgcacgagc gttttaccga caagcaatct gactttcttg cctatctgaa tatttgggac
    23161 agctttcagc gcgagcgcga caaaggtttg tccaacaagc agctggtgca gtggtgccgc
    23221 caatattttc tgtcgcacct gcgtatgtgc gaatggcgcg agctgcacca ccagcttgcc
    23281 caaaccgcga ttgaaatggg tttgaccacc aaggaaaccg ctttcagacg acctcccgaa
    23341 atcaggcagc tcacgtcgtc tgaaaatgcg ggtgaccaag acttatccgc caagctcaaa
    23401 caaaaacaac tggataaaaa gcaacaccgc gcccaaatcc gcgccgccaa agaagcgggc
    23461 tacgaacaaa tccaccgcgc cctgctgacc ggccttatcg ccaacgtcgg catgaaatcg
    23521 cccgacggca acgactacac cggcgcgcgc ggcagccgct tccacctttt ccccgcctcc
    23581 gccctgttca aagccaaacc caaatgggtg atggcggcag aactcgttga aacgacaaaa
    23641 ctttatgcgc gcgacgtcgc cgttatccag cccgaatgga ttgagcagga agccccgcac
    23701 ctcgtccgct accactattt cgagccgcac tgggaacaaa aacgcggcga agtcgtcgcc
    23761 agcgaacgcg tgacgcttta cggcctgacc gtattgccgc gccgccccgt gccttacggc
    23821 aaagttgcgc ccgaagaagc acgtgaaatc tttatccgca gcgcgttggt ggcgcaggaa
    23881 tgcgatttga aagcggattt ttttgtccac aataaaaagc tgattaaaga aattaccgaa
    23941 ctcgaacaca aatcgcgcaa gcaagacgta ttggtcgacg acgaagccct gtttgcgttt
    24001 tataacgaac gcctgcccga aatggcttgg aaagacgcgc aaggcagcgt ttggggaagt
    24061 gaagattccg tacggattat tgaatctgac aaagccgaga ggtcgtctga aaatgagcgc
    24121 aacgagtttc gtaaaaacaa gcgtaatggg tctcgccaaa atgaaaatca cggcaacacc
    24181 gtaggttggg ttgaaaaccc aacatcagcc gcaactgcaa aaactgttgg gtttgacaac
    24241 ccaacctacg ctactcagca acccaccccc tcccccgcga gggagggtcg gggagagggc
    24301 aaaacagttg ccgcacaaac caacttttcc gcaaccgcag caaaccctct cccgcaggag
    24361 agggagcaga gtgccgcagc ttcagcggtt tcaaacgacc tgcatcctgc aaatccccaa
    24421 caaaccgccc cctcccccgt gggggagggt cgggaagagg gcaaaacagt tgcctctcaa
    24481 accaacttct ccgcaaccac agcaaaccct ctccctaacc ctctcccaca ggagggggaa
    24541 cagagtgccg cagcttcagc ggtttcaaac gaccctcaac cccaaaagca gccagcaccc
    24601 caaaaagacc gtctgaaacc cctccccctc gccgacatcc gcaccttcca agcctggctc
    24661 aaaaccgccg aacgcgagaa cccgcgcctg ctgttcctca gccgcgacga tttgatgcaa
    24721 cacgccgccg cacacattac cgaagagcaa ttccccaaat tctggcaaac cgcagacggc
    24781 aagttcaaac tttcctaccg cttcgagccg caccatccgc tcgacggcgt taccctcacc
    24841 ctaccgctga ccgtcctcaa ccgcctgcac gcgccgtcgc tcgaatggct ggtgcccggt
    24901 atgttgcgcg aaaaaatcca gctgctgatt aaagcactgc ccaagcaaat ccgccgcatt
    24961 tgcgtgcccg tacccgattt catcaccaaa tttctcgaaa gcaatcccga ccgccaagcg
    25021 gtcattattc cccagttggc gcactttatc gccaaaagcg caggcgatat gcggattttc
    25081 gagcaaatcg accaagacgc atgggcggcg caagaattgc ccgaacactg ctatctgaat
    25141 ctgctcatca tcgacgacgg cggacaagag cttgccggcg gccgcaaact gcacgaattg
    25201 caacaacagc tcggccaagc cgccgccgtc accttccgcg acaacaccca agaattcgag
    25261 cgcgacaacg ttaccacatg ggatatcggt accctgcccg aatccatcaa attcgcccgc
    25321 ggcaaacaac agctcaccgg ctacctcggt ctgcaaaaag aaaaagacgg ccgcatcgcc
    25381 ctgcgcctgt gtgacaccac cgaagctgcc gaacaggcac accgtcaagg cgtcatcgaa
    25441 ttgatgaagc tgcaattaaa agagcaggta aaagacctga acaaaggcat ccaaggcttt
    25501 acccaagccg ccatgctgct caaacacatc aacgccgaca ccctgcgcga cgacctcacc
    25561 caagccgttt gtgaccgcgc ctttatcggc gaagacgagc tgccgcgcaa cgaaaaagcc
    25621 ttcaaagaac aaatcaaacg cgcccgcagc cgcctgcccg ccgtcaaaga agccctcagc
    25681 cgctacctgc aggaaaccgc cgccgcctac gccgaactga acggcaaact cggcaaacac
    25741 ccattgaccc accttctaag actacgcctg caaaccctgc tcgccgccgg cttcgccacc
    25801 cgcaccccgt gggcacaatg gccgcgcctc cccatctacc tcaaagccat gaccctgcgc
    25861 ctcgaaaaat acagcagcaa ccccgcccgc gacgcagccc gcgaagccga tatacaggaa
    25921 ttggaacaaa tgtggcagga aaaaaacgac ggtttggtga aacaaggact acccgtttca
    25981 gacgacctca ccgcgtttaa atggatgatc gaagaattga gggtgtcgct gttcgcgcag
    26041 gaattgaaga caccgtatcc ggtgtcggtg aagcggctgt tgaaagagtg ggaggatctt
    26101 aattaaagga agatgccgtc taattttttc agacggcatc ttttatttcc ctgaccaacc
    26161 gatttaatcg ctccctacaa atagctttat tttttaattc gcactcccaa acgacaatca
    26221 ctttgaaacc gatcttttcc agctcggttt catttttaat atcacgttcg caattttttg
    26281 tgattttttc caaccaaaaa tccgtattgc ttttcgggat atgtccttta ttacaagaat
    26341 gcccgtacca aaaacagccg tgtataaaca cgactgtttt atatttcgac aaaacgatat
    26401 ccggtttgcc tacatagcgt ttatcgtttt tccgataacg gaaaccttgg aaaaaaagaa
    26461 atttccttac caatacttcc ggtttggttt ctttgctatg gatttttgac atcacgaaag
    26521 aacgtttgga tgtagtgaaa atatcggtca taaattactt ggaagatacc accttcgatc
    26581 gtttttttgt ctgttccact tttcttaaca aatacgaaac caagcttatt aaatccaaag
    26641 catccttatc attaaaaata ctaggagata aatttgctct taactcatgt tgtacaggat
    26701 ttctaaatcc agtccatgtt ccgcatgcaa gctgttcaag accatcttct aaattttgtt
    26761 ccgccttcgt tttgttattc gttaatagaa gtatagaatt tttactcccg aaagcttgtt
    26821 caatcaattc tcttccgtct atgctatgaa gacctgtttg cttctgaacc ttatcttcga
    26881 aaactttaac tgcctcgatt gcagcactta aataatcctt cttttcattg taaaaatcat
    26941 tacaggcagt atgcaaatcc tgatgcaaat gccgccaatg atagtaagga tattctggaa
    27001 taatactatg taaggcagag attacttccg cttgctcttt atttgtaagc tccgaatctt
    27061 ccaaacgatt caaaataact tctactggtt ccctttcctt ttctggcaat ttgtttttcc
    27121 aatcttcgat atcaactccg ctttcgcctt tcacttcgtc atctttctct ttttttcttc
    27181 tttttctcca atcttgcccg atggctcgga cgatctcccc aaggaaaaca cgtaattttg
    27241 aggtttttga atgcttccag tctaatgatg tcctgtcggt agcaattaaa tcttcatcgt
    27301 cattatctat aaagtccaca ttcagccatc cagtcaaata tgaatagaaa tggctggatt
    27361 cgctatcagt gaaaaactcg ggcatattta ccattctgcc gttggcaaac aaggtaatac
    27421 ctttcatatt attctttaaa ggtttttccg ttgtaatgaa cttaccttgt attaatccgc
    27481 tgtattcata ctgttcgaat tttgaagata acgatgaaat attggttgct aaatcctggt
    27541 attcccattc gaattgtggg gtaacaagat catattttag atttccatca atgattttag
    27601 gttcagagcc gttcaagctt actttgattt taaaatcctg agccggaaaa tcaaacaagc
    27661 gggaaagatg ctctacataa ttatctaacg gatatccttg ctttttcgtc agttcggtta
    27721 aagttatggt tgtcccgctt tcgatattgg attcaacaga ttcttttcga aactccggtt
    27781 gataaatacc cttgcttctt cgaatctctg cataatccaa agtaaaagta accctttcgt
    27841 ttccctggat agtagaaatt tcaattttgt tgccaagccc gaataatgcc aatttaccaa
    27901 ggcctttttt acccgttgga attcttccgc acggggaggc ttgtttttct tcccttctgt
    27961 tccgaccgat tctcaaataa aaatcattga tttcatcgaa gctcattcct ataccattat
    28021 ctttaataac gattttatgc tccggtttat cgaataacct aacttccact tccgtagcac
    28081 atgcatcgta ggcattcgct atcaactcgg caatcgcagg aacagtgtgc gaatacatct
    28141 ttacacccaa atgttcgatt acggttggct cgaaactcat tttcaatttt tcttcagaca
    28201 tgttttctcc ttttctaaca ttcgatatta tcaacaattg ccctgcctat tttttccgca
    28261 tacatcggag gaacggcatt accaatcaaa cgggctatct tgtccctgct gcccgcttta
    28321 aagacatagt tacgagggaa cgattgcaat gttgcacctt cgcgcagtga taacgcccta
    28381 tcttcttcag gatgggcaaa cctcccgttg gaaatactaa agaatttggt cgttatggta
    28441 ggtgcgggct tatcccacca tagcctacca aacgtgtctt taaaactatt gtcttttccg
    28501 ataaaacatt ccagctgtaa ttcgggaata tgcgcaaaag ccaaacggtt tcctccgttt
    28561 ttaggtatca aagccaatcg tttcaagttg atatcagata atcccgcaca gctatgcata
    28621 aaatccgttt cgtcttggtg tcctgcaata attttgggaa agccattttc cattcccaaa
    28681 acatcgcgta ccgtaagccg tttgcccgaa tacttgactg gttccagctt ttctttggtt
    28741 attctgtttg caattaacgt aaatctttta cggctttggg gaattccgta atcagccgta
    28801 ttgtgaatac cgaaatggac ggtaaatcct ttttcttcca ataactttat gaaattatca
    28861 agcctgcttt ctttcattct gctcagaatt cccggtacgt tctctacaac aacatatccg
    28921 ggattgaaat attctacaaa ccgctgaaac tccaaaagca aactcttgga tttttccgat
    28981 tttcttttgt ctgtttgaat aacactccaa tattgacaag gactacaacc tataagaatc
    29041 aaatcatcat cattcttttt taacccgagt tctttttcca aggtttcagg ctgcaattca
    29101 aaaacatcag cctgtataaa tttggcattt atattggctt catatgtttc tttgcagctg
    29161 ggttcgtaat ctatcccggc taaaacctga ataccggcgg attgcattcc atagctcatt
    29221 ccacctccgc cacagaaaaa atcaaccgcc tttaacttac tgtttttcat ttctatttaa
    29281 ataattagac tagtgagaac taactttata taacatataa tttattgctt atcaaaaata
    29341 atttgagatt ttattaaaat tcatgcatct aaataagcag aacttaattt cctccaaacg
    29401 agcggatgtc ttcaaatgca cacttcacag aaaggtcgtc tgaaacccat tttcagacga
    29461 cctttttcca cttcccaaaa caagaattcc caccccctcc ccaaacgtcg cccgaaccag
    29521 taaactcccc atatttcaac aacttcttgg aagccaacca tgccccgtat ccaccctcct
    29581 cacctgttcc cgccccatat cgccgaacgc ggccttttgt attttcagca gggcaaggtt
    29641 cgcgatgtcc gaaaaacttc cgccgggcgt tatcgggcgg aggtatgcgg tttggaaaac
    29701 tattgggtat ggctgaagct ggatagtgat ttgtatatta aagacgaaag ctgcgattgc
    29761 ccttatgtct aaaggtgcaa acatacctta aattactata ttacataggc aaaatacaag
    29821 cctataacga attggaaaca aaatgccgtc tgaaaacatc ttcagacggc attataaaat
    29881 ctgttcacct tttcagatga gtaatgtaca cccctataca atttttgcta ctatacccca
    29941 taaatccacg gctaaagata tccttattat gtcctatgat ttatcgaaac gacttgtaat
    30001 cggcttagca tcaagtgccc tattcgactt atccgaatcg gataatatat ttagaatgga
    30061 aggggcagaa acctataggc aatatcagag agaaaaacaa aaccatcccc taaaaaaggc
    30121 gttgtctttc catttattaa aaaacttctg tcaatcaatg aaataaaccc aaacgaccca
    30181 acgattgggt ttattctttt atccagaaac aatccagata cagattacga gtcataacta
    30241 taggcttaat attacacgat tttcattcca tcaaggcgga aaaccgcaca aatactgaaa
    30301 cactatcgat cgatttgtaa acaagcctac ttaagtaact tgcagtcctt atcatttcct
    30361 ttaaaataat ccagcccgtc actacacgaa ctggcggact tcttgcaaat aaaggttact
    30421 agattttcat tcatcttaat aataaaagga tttttatctt tatctatggc taccgccttc
    30481 aacatgaatt tactgtctaa ggccccgcgc gcgattccat tcaaacggat acaaaagcct
    30541 tctgcctctt taatcggcaa acttggccat ttggtagatg tttgtttaaa tctcccattc
    30601 tgcagataaa acttttccat aaaatgtgca ttttctaaca aggctgcccg cactgtattt
    30661 atctttgctt tctcaacata attgcgatag ctcggataaa caattaaagc aagtacagac
    30721 aatatcaaga ccactgatat taattcaacc agcgtaaacc cccgattatc agtcattact
    30781 ttacttccaa taagaacaga ttattcaaca tatttctttg aacagactta ctatcccatt
    30841 cagcagtatg catatttccc act
//
LOCUS       DAWFLT010000019        29438 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWFLT010000019 DAWFLT010000000
VERSION     DAWFLT010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29438
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.019"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..276
                     /locus_tag="WGR74_000836"
     CDS             <1..276
                     /locus_tag="WGR74_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905288.1"
                     /translation="QKANEQEPFIAAVAETLSRTMWGYVMRMDARFEVTFIVDLGHVA
                     FEHQFTAVKQALKEKFEVEADGDALTVESDRLPYGIAACRVVFGDVL"
     gene            273..554
                     /locus_tag="WGR74_000837"
     CDS             273..554
                     /locus_tag="WGR74_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905289.1"
                     /translation="MSGDTPITVEYVFGTLVSFLIALLWYWVKSISDGLKEARKDRDE
                     LLGRLHSVETSYQTKAEAKDNRNEILNLLREIKADLKEVGQKIERKADK"
     gene            554..769
                     /locus_tag="WGR74_000838"
     CDS             554..769
                     /locus_tag="WGR74_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905290.1"
                     /translation="MQNQDPILRALAEINGKQDKLLQNQERMDAEIKKIHADCRRTSA
                     TTGAAAGAISGGIVATGIAFARAKLGL"
     gene            772..1278
                     /locus_tag="WGR74_000839"
     CDS             772..1278
                     /locus_tag="WGR74_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ0905291.1"
                     /translation="MAHPKATRDKLRALYCNGEQSLETAAALCGVSLGTARRWRDEAK
                     AQGDDWNKLRAAHTLAGGSIDEIARAMMTSFLVQYQATMTMLQDAEVEDLPPSKRVQL
                     LASLADAFTKTVAANKRVLPETSQLATALELLQFLMVFVQEKHPKHLAAFVEVLEPFG
                     AEVERKFG"
     gene            complement(1275..1469)
                     /locus_tag="WGR74_000840"
     CDS             complement(1275..1469)
                     /locus_tag="WGR74_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905292.1"
                     /translation="MKSIDLEISKLLDAGKYTPSEIQDLLEEQGFKISLKKLADHLDL
                     LVAIGVAGKHSDDTFTSRLN"
     gene            1481..3100
                     /gene="terL"
                     /locus_tag="WGR74_000841"
     CDS             1481..3100
                     /gene="terL"
                     /locus_tag="WGR74_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ0905293.1"
                     /translation="MKNKDFLKSLSALAANLRQVIEAEVDGFDASPTAVAERRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSDLHRFLFRRLPEIVESPEGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGRKHYCVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFADVCGQGRV
                     WQAGTIVTANDIKVQVAGSGKKLRGLRHGPYRPDLTILDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWKGIKFKAMLKWPDRMDLW
                     DRWEELYRNEGELVADAFYQTNKAEMERGAETSWAARGVLALMKIRARDGHAAFDSEY
                     QNDPVSGEDAPFAQAMQFWAELPADLVYFGALDPSLGKAGASRDPSAIIVGGYQRSSG
                     KLFIVEAQIKKRLPDLIIEDVIRLHAQYKCKLWFVETVQFQEFLKDELVKRSAAKGIP
                     VPARAVKPIADKLLRIETLQPHMANGLILLHANQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGATANSAPVEYMAVTKHGTDGGFGSGAW"
     gene            3179..4738
                     /locus_tag="WGR74_000842"
     CDS             3179..4738
                     /locus_tag="WGR74_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ0905294.1"
                     /translation="MKNIFSSALSKILPGRYTPKSTPQTAEITQNTQTHEHPSKGLTP
                     QKLHGILEAAERGDMKAQSELFADIEEKDGHIFSEMSKRKRAVIGLDWRVMPPPNSTD
                     AERRLAEEVKGWIERLPDFEDMMFDLLDAVGHGFACVEIEWQQIGGLWLPKNFIHRPQ
                     GWFKVDGADNVRLAKQDNPDGEELWAFGWLVHKHRSRSGLLVRGGLMRTLVWPYLFKN
                     YSVRDLAEFLEIYGLPTRLGKYAVGADETDKTTLLRAVKEIGHNAAGIIPETMNIELL
                     NAANGSSEPFMAMIDWADKTSSKAILGGTLTSMADGKTSTNALGQVHNEVRHDLLVSD
                     AKQLAGTITQQLILPLLRLNKGNVDETCLPRFQFDTQLPEDMAVYAESLPRLVEMGMK
                     IPLAWAQEKLAIPLASDDEPVLALQTAESEGVKVAPLSYRRVALSRQGEILDMGQAAI
                     DNAGLGKIALPEHIEPFLRGLGQALAEGDSYEEVQERLLRAYPHLDSAEFQTALARVI
                     FISDLWGRLNG"
     gene            4731..6077
                     /locus_tag="WGR74_000843"
     CDS             4731..6077
                     /locus_tag="WGR74_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEZ0905295.1"
                     /translation="MADLGFAFGLEPEAAVKYFEGLGYHIPPDWDVKWNEAQTKARTI
                     AGIHRQDIVGEFHAAMYEAAKSGKSFEAWRDEVQGRLKAHDWHLLKDGDIVDGDTGEV
                     LGRGITKHRMETIFRTQMQSAYMAGHWQAFEEGRDDAPWLQYSAILDSRTRQSHAAAH
                     GAVYHIDDPFWDYFYPPNGFNCRCTVRALSDSDLKRRNLLPQKAQLEDTEVVVNRKGD
                     TRPAKAVKLPDGRRFYTDAGFQHNVGKSHLANLGQLQMQRAVELPPKLASVSIQTALK
                     QPDLMRAVSQQAAQMVRRVDAEKVARGKTLYVGALALPVLDALAARKIYPQSAVIAIS
                     DERVLHALRDKKVKPLPVSFWEKIPELLQEPEQILLGKAGRNDDAKQFLAFVYPLSAG
                     KGKLVVTLDYDVKTRHPLTGKKEHLTLNMVNTGMIAETDKQVDSLLYGYETIWKKP"
     gene            6181..6678
                     /locus_tag="WGR74_000844"
     CDS             6181..6678
                     /locus_tag="WGR74_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ0905296.1"
                     /translation="MIEIKINTDTLQNSLNAAARHTSHTKPLMTRLARIMRNAVLENF
                     AAGGRPAWAPRKYPTAREGSGLLQASGRLRNSITPSSTNDTAVVGTNVEYAAIHNFGG
                     KTSPHLIRPKKGKALKFGGRFAKQVNHPGSNIPARPFMTLQPEDEKALSDAVAEYLAQ
                     AIQGR"
     gene            6887..7951
                     /locus_tag="WGR74_000845"
     CDS             6887..7951
                     /locus_tag="WGR74_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ0905297.1"
                     /translation="MRAMDTKTLLAALSAAHVGGSDGLIKIVPKGQFAPVDGRTDTGV
                     PHWTMSADLAQQIIAAFDAAQTDLVVDYEHATLKAAETGQQNPAAGWISKYVWDDERG
                     LMGEVKWTQRAKDMIDSGEYRYLSPVLEYDTLGNVRGLHSVALTNSPALDGMALAALS
                     RQNSINPKQETSMNKEALIKLLGLAADADDKAIEAALAEAQEKLGGKTLAEALAAPKE
                     EPQGSEGDKGDAGKPEDKPQGGNADDGEVAELKAQVAALSKKVIAMEVGGTSDGLIRA
                     ALSDGRLLPHQEASARQLAAKDPEAFKALIDGSLALAALSKTQTGGKGGADGTSALTA
                     EEAAVAAQLGISAEDYAKAK"
     gene            7973..8875
                     /locus_tag="WGR74_000846"
     CDS             7973..8875
                     /locus_tag="WGR74_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HEZ0905298.1"
                     /translation="MIITPDTLKALFTGFKKNFQDGLKMAESQYKEIATVIPSSTASN
                     TYGWLGQWPAFREWVGDRVFQDMKAHGYAITNKHFESSVKVNRNDIEDDNVGIYAPMM
                     TEMGRASAVHPDELVFALLKNAHATLCYDGQNFFDTDHPVYEKVDGTGQASTVSNIFA
                     GSEAAWYLLDTTRALKPLIYQERKAKQFTAMTADTDEGVFMRNEYRYGVDGRCNVGLG
                     FWQMAAKSQEKLDAAGFEKAYNAMVSLKGDGGRPLAIRPNVLLVPPALENAAKELVEG
                     DRLANGAYNPNKGKAKVIVSPWLL"
     gene            8913..9386
                     /locus_tag="WGR74_000847"
     CDS             8913..9386
                     /locus_tag="WGR74_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905299.1"
                     /translation="MAKEKNGQEVGATVDVQPEEVTVTAALQAEIEALRAELDKANAE
                     IQVAQAELAAAQERNAKLERLLEAGLAPGGISDGETLTPEAEAAFYTGSGAPAADAEV
                     VAIKSKHGHAFFRAGYHVQPNWTFVRRADFEPADFERLIGDRMVEAREALPTDAS"
     gene            9383..9808
                     /locus_tag="WGR74_000848"
     CDS             9383..9808
                     /locus_tag="WGR74_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HEZ0905300.1"
                     /translation="MSYAAVADLVARFGEATITGLTDLSRKGAVDETVAQQALDDAAA
                     EIDGYLMNRYTLPLPKPLRILTVYCCDIAVYRLCTGKRQLTEDIVHRYEAAVKFLQLV
                     AAGKVGLGVTEPAGEKPAVQGNGVMFTTQEKVFGRDSVY"
     gene            9792..10460
                     /locus_tag="WGR74_000849"
     CDS             9792..10460
                     /locus_tag="WGR74_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ0905301.1"
                     /translation="MIASIEQAIRQRLSDGLGQMVTGVFTYGGEFDGEGLAQVVNQFP
                     AVWVMFAGIKDTIRHDTRGSRFKAIGQFTVLVGDRASGSEADSRFGGLHRHDVGTYRL
                     MQTCRHLLTNQSLGLQIDRLQPGAAKSLFSRQMEQDAVSVFALEFETYWFEDALQDGD
                     WPRPAVSDGQQAKVYADVSEYQGRTEPEHPDFKGVNLELRIPPKTPDQPADMAATVET
                     KVKP"
     gene            10457..10645
                     /locus_tag="WGR74_000850"
     CDS             10457..10645
                     /locus_tag="WGR74_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ0905302.1"
                     /translation="MNETIKVRAADGLQVPLAGKPHEYITDQETVTVPDAAYYRRCIE
                     YGDLVVVTEDTQPAKGSK"
     gene            10645..12072
                     /locus_tag="WGR74_000851"
     CDS             10645..12072
                     /locus_tag="WGR74_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0905303.1"
                     /translation="MASANINFEKIPASTRKPGVYAEWNTKLAVRNLPTNKQRVLIVA
                     QHNNPALGELTELENVFSAADAAAKYGAGSMAHLMVTAAIKAYAYADLSLITVADNKA
                     GVAAGGKITLSGTANTQGVLRVSIANADTLTIGIGAEDTAATVAAAVKAAIDAVPDLP
                     VTATVAEAVVTLTAKNKGTAGNAIRIKTSNTAEGITAAVTAMTGGDANPDIAAALNAV
                     VAEGHHIIACGINDETNLLKLRAHLDTVASPMEKRWAICVYGQTGTLAQATTLAGRLN
                     HGHIVSAWYRGTPSLPCELAAAFAAVMASEEDPARPLNTLALNSIGVCESKDKTMRTE
                     QENALYNGVTPIETSPAGTQAQIVRAITTYTKTANGTADESLLDVTTVRTLIYVSRAC
                     VDRIALRFPRDKLSDRTPPRVRSELIDVLMCCEELEILERVEENLPKLIVERDLQNTG
                     MLNCRIPSDVVNGLHVVGMVVDLYL"
     gene            12086..12460
                     /locus_tag="WGR74_000852"
     CDS             12086..12460
                     /locus_tag="WGR74_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0905304.1"
                     /translation="MSTEYVGSVILYLGANEVEVTKIDVKDNTGKKPVKTMNRTRRVK
                     GFTRGVGQYDITFTAVVPTDGTAVDWAKIDDAKISLVPDVEGARPTSYLGFCATEAGD
                     SYTVDNELVVDVTGFAIRKVLE"
     gene            12636..13022
                     /locus_tag="WGR74_000853"
     CDS             12636..13022
                     /locus_tag="WGR74_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0905305.1"
                     /translation="MGLTKFGAAVREARRQTKQTLQTMAATLGTSPAFLSAIETGRSK
                     VPMDFVKKVEDFFENLGQPIDGLKQKAMVSNENVSLSGLSLQQQMLVAGFASSDFSKE
                     QLDKFAELLRTIHHKDSQKEGDDAAN"
     gene            13009..13473
                     /locus_tag="WGR74_000854"
     CDS             13009..13473
                     /locus_tag="WGR74_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEZ0905306.1"
                     /translation="MQQTNYSMRGVRVGMLSEETIQFFANEAAKFLVVDKRTRKKMDA
                     FMEMLEEYGIVIDVVADSEWLVITNAMCHNGTILMPNSLYTRICNGEDEAIFIFFHEL
                     GHLLLGHKAMLHYSEVLPTKQEDSEWQADEFAKCILRNMGIKYVPEQLSLRF"
     gene            13655..13834
                     /locus_tag="WGR74_000855"
     CDS             13655..13834
                     /locus_tag="WGR74_000855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905307.1"
                     /translation="MMATIFTIGGAIMAMTKAPVTPDLVDDTGAYIFRKSFTTKSGKK
                     IVSKNGKPFKIPVKV"
     gene            13963..14352
                     /locus_tag="WGR74_000856"
     CDS             13963..14352
                     /locus_tag="WGR74_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905308.1"
                     /translation="MSEFSPELTRAIEDYELRVSPDLKTVAGRLKYGIGVIDGEMHHD
                     FSMHLLTVREDMAIDPQLEGQPRLVAAYAASLDKLGGLTAESLTPDLLLDEMAAADFD
                     ALYWAQELLQKKRLCPHPAPTVTDTPS"
     gene            14310..14558
                     /locus_tag="WGR74_000857"
     CDS             14310..14558
                     /locus_tag="WGR74_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905309.1"
                     /translation="MPAPRADRYRYAVLKLGRYGITPAQIDGMTQPELEGWLKQADLI
                     ERGRAAPVVMPWFIPANVKPSAATGGQTQTFISKRKKK"
     gene            14555..16604
                     /locus_tag="WGR74_000858"
                     /pseudo
     CDS             14555..16604
                     /locus_tag="WGR74_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            16604..17971
                     /locus_tag="WGR74_000859"
     CDS             16604..17971
                     /locus_tag="WGR74_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ0905310.1"
                     /translation="MAWKDTLLDAGFKGVTFDVIDDTLRGTHALAEHEYPFVQGADIE
                     DTGVSAMDMALTAVLWGEDYEGRLQNLLNVLCETGAGELIHPIYGSVPDCVVADFEVA
                     HNEENPDYCTVRMTFKQSVKAAPFFDRDLPIALADEVDFLADLAAWQGFEVFQTALNK
                     IQKAQSRWNAFHATVLMVVGVLYGQVNGIFTGGLNLLNSPRVLVAELKSVFGALANMH
                     NVGKSGLDGWRDMVGGVSKAAATPWQVSRGTEGSVAAVDLIQRAKPEDVAAFTALTAT
                     VGACALAEQAADILAVQLNEPTLTPVEISRLLADTRAALQRALAAQRILAMMLADETK
                     ADKLAYCLLRLYQTPVDSADDVYQRIEVAGLLPQAPYLETAAALTESLRNTAHKLQKQ
                     AFTVINMKPPLVQKTVTCDIGLHLLAFEWYGDYSRFGELLRLNPQIRHPNFIEKGTVL
                     NAYAR"
     gene            17955..19094
                     /locus_tag="WGR74_000860"
     CDS             17955..19094
                     /locus_tag="WGR74_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0905311.1"
                     /translation="MPTPDNTVTLLINGKTHGQWTNYDIVSDLLTPADDFSVMLGRPV
                     DAKPDAVRAGDKVEVRVGGDTVLSGRIDRVQTVTEKGGKTLTIQGRDDAGVLLDCSAP
                     LFNAQDMDLNQIIEKIVKPLGLAKIRIDAAKTDKTHKVQIEPGSRAWDALLEYAEANG
                     LWPWLEPDGTLVVGGPDYTAAPVAELVLRTNGQNNNIKRLEVNRDMAARYSEVTVLAQ
                     SHSGKNNIKATAKDESVKLHRPLIVTEPDIDSQAQAQRKAKKRLADSRLEGLTITATV
                     QGHRTDDGTLWQPGQRINVLSEPDGIDAVYFLMARTFIGGRGQPTETVLTLKEDGAWV
                     LDADPPKKSGKTKRPSESRKANGHAAAKPKKRRQAKQAGQELQVI"
     gene            19095..19724
                     /locus_tag="WGR74_000861"
     CDS             19095..19724
                     /locus_tag="WGR74_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEZ0905312.1"
                     /translation="MDAKTIDNRIRRAFNGIRQAFRGKIARVKAAGGVQQIQVEGLEG
                     ETVQDLEHAENFGFTSNPPAGSDCVVVPLGGKTSHGIIVTTTNGAYRITGLAEGETAV
                     YNADGAKMVLKKGRIIEIDCETLNIKAPGGVNIDAPNVGCTAQITAEGQINGNGGMAV
                     KGGSGTSFTGNVQMVGDLDTTGKLTNNGKNVGSDHKHNGDSGGTTSDPL"
     gene            19785..20138
                     /locus_tag="WGR74_000862"
     CDS             19785..20138
                     /locus_tag="WGR74_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HEZ0905313.1"
                     /translation="MDALLNPQTGGYVVNQSAQSIENELYIRLVTPLGSYWADRTLGS
                     RLHELRRQKHLKRIEVLAKQYAEQALQPVIQSKRAQSIQVTASAPQHGWLKLHIEAVD
                     AAGDTVTLNHKVAVI"
     gene            20135..20620
                     /locus_tag="WGR74_000863"
     CDS             20135..20620
                     /locus_tag="WGR74_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ0905314.1"
                     /translation="MTQALNLEQIRANYLRDLQNQNPAAHVHAGSDNHVRATAIAAVG
                     EGQYQHQEWILRQAFADTADSAYLEKHAAKYGIYRKTATFAGGKVRVRGAVGATVPVG
                     QQINVGDKVYLTAESAVISALGSAEIAVIATVAGSAQNQTAETAATLQSVPAGIDSSA
                     V"
     gene            20623..21198
                     /locus_tag="WGR74_000864"
     CDS             20623..21198
                     /locus_tag="WGR74_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ0905315.1"
                     /translation="MVGGTDAESDESLLARYEERLRRPAAGGNQYDFRNWCLEVPGVV
                     DAFIYPLRRGNGFVDAVILGENGIPSAETLAAVQAHVDAVRPVTRKNGFLALAPSIQT
                     VNVAVTITLSSGTDTDTATAAIKSAVNAYFDALKPGDPLIKSQLETLISEVYGVRDRV
                     LTTPVGNIKPQESAEDIYWLRPGSINVEYTT"
     gene            21195..21761
                     /locus_tag="WGR74_000865"
     CDS             21195..21761
                     /locus_tag="WGR74_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ0905316.1"
                     /translation="MIHQTLLAAMRPPVSYDTVGETAEIKAEAGVFDIVADHAEGVKN
                     APFPDAENDYLYRWEELLAITPPAGANTQQRTDAVLAKLNALGGLSIAYFTAIAESAG
                     YTVTIYEEDQFRAGESCAGDCLNTEDAIWRWCVDIADGKATAYIFRAGQSRAGDRISV
                     YTDPIIETMFEELKPAWTYCRFEYEEEV"
     gene            21764..22999
                     /locus_tag="WGR74_000866"
     CDS             21764..22999
                     /locus_tag="WGR74_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905317.1"
                     /translation="MDLIQTPNKQFVDGDRRTPGTPVPAWWLNQLQGELYSILNAVGI
                     EPNKADHAQVLSAIKTLAADASQVASIDALRKYSGTGYVNVNAYHANTTVGGGVFVAD
                     KADKSTADNGCTVIVSTDGTRWKRVFSGMLNLHDFGYVASKNNALSTLNAAESAALDV
                     VVDCLGLSIDTGNTYPQKNKYTNGKFVINGKTVDVQYQPIRSGIGRFISGTGAAANLK
                     SNEWTGAGLIVIGEGAMEQMEKCVSSIAIGDRAQGFSKVSRDNIAIGADSLINVQAAT
                     EWYDQSRMEGTRNIGIGGNAGRGITSGYSNVSIGRNAGQGLGEGSSNIALGAGAMAGT
                     APVGFSGDIEVFWPSSTSRTIAIGEAVLQTYQGRAAQTAIGANAARNTKKAEKVTAIG
                     SAAMENLERNRAPNGGDVV"
     gene            23162..24046
                     /locus_tag="WGR74_000867"
     CDS             23162..24046
                     /locus_tag="WGR74_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEZ0905318.1"
                     /translation="MNGNTLIIQSSKELTATGAAELKYVYSVNSTATKNEELTIIGAN
                     AMNKALTAGYSTIIGADAALLGDNYQKTTAIGASSLRTGSHISTTAIGYWVIPLASSE
                     KCVAIGDSAGYRNVQGDFLTGKITNSIAIGYGARINGDNEIQIGTTGQTLYAPTAVNI
                     RSDGRDKADVKPLTNGLDFVMKLKPMTGYYDRRDSYVDELFKDLPADERADKVREWWA
                     NPIKDGSHKEDRLRHWFIAQDIAALEDEYGRLPMVNKTNDTYTVEYETFIPVLTKAIQ
                     EMAARIETLETEMKESKK"
     gene            24043..24666
                     /locus_tag="WGR74_000868"
     CDS             24043..24666
                     /locus_tag="WGR74_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905319.1"
                     /translation="MTRCVIDQDGLFVEEQYFDDGRQSIEAEMPDLAQYQAAQWDGQG
                     WKIIPDYRGCVVFAGGQEQVWDKLGDLPDGVSLTPPESVNIDGLKSVKLVALNAAAQA
                     FINKHAGIDSVPEFEFASWAIQAAEAKAWQEDKAAPTPVLDGIATARGMSADTLKAAA
                     LRKTLAYEQLAAHVAGQRQALQSKIEAAKTQAALDKIAVVFTLPEAV"
     gene            24667..25155
                     /locus_tag="WGR74_000869"
     CDS             24667..25155
                     /locus_tag="WGR74_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905320.1"
                     /translation="MVQVYLALYKGKAAINTPRDVVKRIADSVVRLATCSPYSHCEIA
                     VKHPRDGLFDCYSSSARDGGVRIKTMPLPADKWDLIPLPQSVAISASRLFHRTHGAGY
                     DWLGAIGVVLKSPHSKSRWFCSEWCAYVIGYTNPCRYSPQTLYAAVSTKDRPSEKMEE
                     TK"
     gene            25503..26342
                     /locus_tag="WGR74_000870"
     CDS             25503..26342
                     /locus_tag="WGR74_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEZ0905321.1"
                     /translation="MMQKYHSTAPLPFVGQKRYFIKHFTKVLSQIPADGKHWTIVDVF
                     GGSGLLAHVAKRIKPQARVIYNDYDNYSDRLRHIPDYNRLREQIAQIVGGIPKGSRLD
                     PERTRSVQQTITNFQGHIDVRVLSSWLLFSAKQANSLEQLLGFEFYNKVRQSPYSIAA
                     DYLDGLEITQQDYNLLMAEHQHNPNTLLVLDPPYVSTAQGAYAADKYFNMVSFLRMIQ
                     YMRPPFILFSSTRSEALDYFQFLQECEPDKYRRFSGYNIVSLDAKMGKGIEYQDNMIY
                     KID"
     gene            26602..27135
                     /locus_tag="WGR74_000871"
     CDS             26602..27135
                     /locus_tag="WGR74_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ0905322.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(27198..28382)
                     /locus_tag="WGR74_000872"
     CDS             complement(27198..28382)
                     /locus_tag="WGR74_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ0905323.1"
                     /translation="MTEPTSRRRFLKTCTAAIGAGLLQACGTSATSVPPLPSFHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRF
                     QRFAGTDSQRAADFQDVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(28422..28826)
                     /locus_tag="WGR74_000873"
     CDS             complement(28422..28826)
                     /locus_tag="WGR74_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ0905324.1"
                     /translation="MTAQPCPICTAQNEDVLLQTPKLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFQPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         7504 a         7661 c         8232 g         6041 t
ORIGIN      
        1 caaaaggcca acgagcaaga gccgttcatc gcggcggtgg ccgaaacttt aagccgcacg
       61 atgtggggct atgtgatgcg gatggatgcg cggtttgaag taacgtttat cgtagatttg
      121 ggccatgtgg ccttcgagca tcaattcact gcggtcaaac aggcgctgaa ggaaaaattc
      181 gaggttgaag ccgatgggga tgcgctgact gtggaatccg accgcctgcc ctatggtatt
      241 gcggcctgcc gggtggtgtt cggagatgtg ttatgagcgg agatacaccg attaccgtgg
      301 aatatgtgtt cggcacgttg gtgtcgtttc tgattgcttt attgtggtat tgggtaaaaa
      361 gcatttcaga cggcctgaaa gaagcccgca aagatcgtga tgaactgctc ggcaggttgc
      421 acagcgtaga aacatcttat cagacgaaag cggaagcaaa agacaacagg aacgaaatct
      481 taaacttgtt gcgtgaaatc aaggctgatt tgaaagaagt cggccagaaa atcgaacgga
      541 aggcagacaa ataatgcaaa accaagaccc tattttaaga gcgttggcag aaatcaacgg
      601 caagcaggac aaattgttac aaaatcagga acgcatggat gcggaaatca agaaaatcca
      661 tgccgattgc cgccgcacgt cggcaaccac cggcgcggcc gcgggcgcga tttcgggcgg
      721 tattgtggct acgggcatcg catttgcccg cgccaagttg gggctgtaac gatggcgcat
      781 ccgaaggcga cccgcgacaa gctgcgggca ctctactgca acggcgagca gagccttgag
      841 acggcggcgg ctttatgcgg cgtatcgctc ggcaccgccc gccgctggcg cgatgaggcc
      901 aaggcgcagg gcgacgactg gaacaagctg cgcgcggccc atacgctggc cggcggcagc
      961 atagacgaaa tcgcgcgggc gatgatgacg tcgtttctgg tgcagtatca ggcgacgatg
     1021 acgatgttgc aggatgcgga agtggaagat ttgccgccga gcaaacgggt gcagctgctg
     1081 gcgagtttgg ccgatgcgtt taccaaaacg gtggcggcga acaagcgggt gttgccggaa
     1141 acctcgcaac tggcgacggc tttggagttg ttgcagtttt tgatggtatt cgtgcaagaa
     1201 aaacacccca aacatttggc tgcctttgtg gaagtgctgg agccgttcgg ggcggaggtg
     1261 gagaggaagt ttggttagtt taatcgtgaa gtaaacgtgt catcagaatg tttaccggca
     1321 acaccaattg caaccaatag atctagatga tcggctaatt ttttcagtga aattttaaag
     1381 ccttgctcct ccaataaatc ttgtatttca gacggcgtgt atttgcccgc atctaacagt
     1441 ttggatattt ctaaatcaat cgatttcatt ttttgccctt atgaaaaata aagatttcct
     1501 caaatccctg tccgccctcg ccgccaatct gcgccaagtc atcgaggccg aagtagacgg
     1561 tttcgatgct tcgccgacgg cggtggccga gcggcgggcg aaggtatttg acccggtagg
     1621 cggttacgag tatttcgtca acacctactt cccgcattat atccgttcgc ctgaaaaatc
     1681 cgacctgcac cgcttcttat tcaggcgttt accggaaatc gtcgaatccc cggaaggcga
     1741 gaacgaagcg gttggcgcgc cgcgcggcga gggtaagtcg acgcaggtga cgcagttgtt
     1801 tactttgtgg tgcattgtaa cgggacgcaa gcattactgc gtgattgtga tggacagcat
     1861 agaccaggca tacccgatgc ttgaggccat caaagcggaa ttggagttta atccccgcct
     1921 gaaaaccgat tttgcggatg tctgcggcca aggccgcgtg tggcaggcag gtaccatcgt
     1981 aacggccaac gacatcaagg tgcaggtagc gggcagcggc aaaaagctgc gcggattgcg
     2041 ccacggccct taccgccccg acctgacgat tttggacgat attgaaaacg acgaacaagt
     2101 ccgaaatccc gaacagcgcg acaagctcaa tgcgtggctg acgaaaaccg tgctgccgct
     2161 cggtggtgtg gggcaaaaat acgatgtgat ttatatcggc acgattttgc attacgacag
     2221 tgtgttgaac cgaacgctca ataatccgtt ttggaaaggc atcaagttta aagccatgtt
     2281 gaaatggccc gaccgcatgg atttgtggga cagatgggaa gagttgtacc gcaatgaagg
     2341 tgagttggtg gccgatgcgt tctatcaaac gaacaaagcg gaaatggaac gcggggcgga
     2401 aacttcttgg gcagcgcgcg gcgtgctggc tttgatgaaa atccgcgccc gcgacggaca
     2461 cgcggctttc gattcggaat atcagaatga tccggtgagc ggtgaagatg caccgtttgc
     2521 ccaagccatg cagttttggg cggaactgcc cgccgatttg gtgtatttcg gcgcgctcga
     2581 cccttcgctc ggcaaagcgg gggccagccg cgacccgagt gcgattatcg tgggcgggta
     2641 tcaaagaagc agcggcaagc tgtttatcgt ggaagcgcaa attaagaagc gtttgcccga
     2701 tttgattatt gaagacgtta tccgcttgca tgcgcaatat aagtgcaagc tgtggtttgt
     2761 ggaaacggtg cagtttcagg agttcctgaa agacgagctg gtaaaacgaa gtgcggcaaa
     2821 aggcattccc gtgccggcgc gggcggtcaa accgattgcc gacaagctgc tgcggattga
     2881 gactttgcag ccgcacatgg ccaacgggtt gattttactt catgccaatc agcaaacctt
     2941 aatccaacag tttcgccatt ttcccaaggc agaccatgac gacggccccg atgcggtgca
     3001 tatgttgtgg tcgggggcaa cggccaacag tgcgccggtg gaatatatgg cggtgacgaa
     3061 acatgggacg gacggcggtt tcggcagcgg cgcatggtga aactgacacc gggcagggct
     3121 tacgattaat gcaggcagca gagaattggg gcaagtttaa cttgcccttt ttttatttat
     3181 gaaaaacatt ttcagcagtg cattgagcaa aattctgccg ggccgctata caccgaaatc
     3241 taccccgcaa acggcggaaa ttacccaaaa cacccagact cacgaacacc cgagcaaagg
     3301 tttgacaccg cagaagctgc acggcatttt ggaagctgcc gagcgcggcg atatgaaggc
     3361 gcagtcggaa ctctttgccg acatcgagga gaaagacggc catatctttt ccgagatgag
     3421 caagcgcaag cgggcggtca tcgggctgga ttggcgcgtg atgccgccgc cgaacagcac
     3481 cgacgccgaa cggcggctgg ccgaagaagt caagggatgg attgagcgtc tgcccgattt
     3541 cgaagacatg atgttcgacc ttttagatgc ggtcgggcac ggctttgcct gtgtggaaat
     3601 cgaatggcag caaataggcg gcctgtggct gccgaaaaac tttatccacc gtccgcaagg
     3661 ctggtttaag gtggacggtg ccgataatgt gcggttggcc aaacaggata atccggatgg
     3721 ggaagagctg tgggcgttcg gctggctggt acacaaacac cgcagccgtt cgggtttgct
     3781 ggtacgcggc gggctgatgc gcacgctggt ttggccgtat ctgtttaaga attattcggt
     3841 gcgcgatttg gccgagtttc tggaaatcta cggcctgccg acgcgtttgg gcaaatatgc
     3901 ggtgggggcg gacgaaaccg ataaaaccac gctgctgcgg gcggtaaagg aaatcggaca
     3961 caacgccgcg ggcattatcc ccgaaaccat gaatatcgaa ttgcttaatg ccgccaacgg
     4021 cagcagcgag ccgtttatgg cgatgatcga ctgggcggat aaaacatcgt cgaaagcgat
     4081 tctgggcggt acgcttacca gcatggccga cggtaaaacc agtaccaacg cgctgggtca
     4141 ggtgcataac gaggtgcgcc atgatttgtt ggtgtcggat gccaagcagc ttgccggtac
     4201 gataacgcag caactgattc tgcccctgct gcggctgaat aaaggcaacg tggatgaaac
     4261 ctgcctgccg cgtttccagt ttgatacgca gctacccgaa gatatggcgg tttacgccga
     4321 atctttgcct aggctggtag agatgggcat gaagattccg ctggcgtggg cgcaggaaaa
     4381 actggcgatt ccgctggctt cggacgacga gccggtattg gctttgcaga cggctgaaag
     4441 cgaaggtgtc aaagttgcgc cgttaagtta ccgccgcgtg gccttgagca ggcagggcga
     4501 aatcttggat atggggcagg cggccatcga taacgcaggt ttgggcaaaa tcgccctgcc
     4561 cgagcatatt gagccgtttt tgcgcgggtt gggtcaggct ttggccgagg gcgacagcta
     4621 tgaagaggtg caggagcggc tgttgcgtgc ttatccgcat ttagacagcg ccgaatttca
     4681 gacggccttg gcgcgggtga ttttcatctc cgacctgtgg gggcggctca atggctgatt
     4741 tgggttttgc cttcggtttg gagccggaag cggcggtcaa atattttgaa ggtttgggct
     4801 accatatccc gcccgactgg gatgtgaaat ggaatgaggc gcagaccaag gcgcggacga
     4861 ttgcaggcat acacaggcag gatattgtcg gcgagtttca cgcggcaatg tatgaggcgg
     4921 cgaaatcggg caaatcgttt gaggcttggc gcgatgaagt gcaaggccgt ctgaaagcgc
     4981 atgactggca tctgctcaaa gacggcgata ttgtggacgg agacaccggc gaagtcctcg
     5041 gacgcggtat taccaaacac cgcatggaaa cgattttccg cactcagatg caatcggctt
     5101 acatggccgg tcattggcag gcgtttgaag aaggtcggga tgatgcgccg tggctgcaat
     5161 attcggccat tttggacagc cgcacccgcc aaagccatgc ggcggcgcat ggtgcggtgt
     5221 atcacatcga cgacccgttt tgggattact tctacccgcc caacggcttc aattgccgct
     5281 gtaccgtgcg ggcgctttca gacagtgatt tgaaacggcg caatctgctg ccgcaaaaag
     5341 cgcagcttga agatacggaa gtggtggtca accgcaaggg cgatacccgc cccgccaaag
     5401 cggtaaagct gccggacggc cgccgctttt ataccgatgc gggctttcag cacaatgtcg
     5461 gtaaaagcca tttggccaac ttggggcagt tgcagatgca gcgtgcagtg gaactgccgc
     5521 cgaaactggc gagcgtatcg attcagacgg ccttaaaaca gcctgatttg atgcgggcgg
     5581 tatcgcaaca ggcggcgcaa atggtacggc gggtggatgc ggaaaaagta gcgcgcggga
     5641 aaacgctgta tgtgggcgca ttggccctgc ccgtgttgga cgcattggcg gcaaggaaaa
     5701 tttacccgca atccgccgtg attgccataa gcgacgagcg ggtattgcac gctttgcgcg
     5761 acaagaaggt caagccgctg ccggtatcgt tttgggagaa gatacccgaa ctactgcaag
     5821 agccggagca gattttattg ggcaaggcgg gacgaaatga cgatgccaaa cagtttttgg
     5881 catttgtgta tccgctgtcg gcaggcaagg ggaagctggt ggtaacgctg gactatgatg
     5941 tgaaaacccg acatccgttg acaggtaaga aagaacatct gaccttgaat atggtcaaca
     6001 ccggcatgat tgcagaaacg gataaacagg tggatagctt actgtacggg tatgaaacaa
     6061 tatggaaaaa gccataaaac tctttgcctg attcgaacag gataatacgc gacacggtaa
     6121 cgtggccgta acctttccag taggaaaccc gagttttatg gctgttggaa ccaatatacc
     6181 atgattgaga taaaaatcaa cacagacacc ctgcaaaaca gcttaaacgc tgcggcgcgg
     6241 catacctccc acaccaagcc tttgatgacg cggcttgccc gcatcatgcg caacgccgta
     6301 ctggagaatt tcgccgccgg aggccgtccc gcttgggcgc cgcgcaaata tccgactgcg
     6361 cgtgaaggtt cggggctgtt gcaagccagc ggccgcctgc gcaattcgat tacgccgagc
     6421 agtaccaacg atacggcggt ggtcggcacc aatgtggaat atgcggctat ccataacttc
     6481 ggtgggaaaa cctcgccaca cttgattaga ccgaaaaaag gcaaagcgtt gaaattcggc
     6541 ggccgctttg ccaagcaggt taaccacccc ggcagcaata ttcccgcacg cccgtttatg
     6601 accctgcaac cggaggacga aaaggcccta tccgatgcgg tggcggaata tctggcacag
     6661 gccattcagg ggcggtaatc atgcctgcct gaaaaatgcc gaaaaacagc ccgaaaacgc
     6721 cctaacccat acttacccct acccatcgcc tgaaaatcaa tcctgcgcgc gtttgaacac
     6781 cttttgaaca ctatcccacg cgtatctctg caagtacatt tcccttccct agaaattcat
     6841 ctgtctgaca taggccaagc tttgacgagg gcgcggctgc cgcacaatgc gggctatgga
     6901 caccaaaacc cttcttgccg ccttatccgc cgcccatgtc ggcggttcgg acggcttaat
     6961 caaaatcgta cccaaaggcc aattcgcacc ggttgacggc cgcaccgata cgggtgtgcc
     7021 gcactggacg atgtctgccg atttggcaca gcaaatcatt gccgcctttg acgccgcgca
     7081 aaccgacctt gtggtcgact acgaacacgc cacgctgaaa gccgccgaaa cggggcagca
     7141 aaaccctgcc gccggctgga tcagcaaata tgtatgggat gacgagcgcg gcctgatggg
     7201 cgaagtgaaa tggacgcaac gcgccaaaga catgatagac agcggcgaat accgctatct
     7261 gtcgccggta ctcgaatacg acacgctggg caatgtacgc gggctgcaca gtgtggcgtt
     7321 gaccaattcg cccgcactgg acggcatggc tctggctgca ttgagccgcc aaaactctat
     7381 caaccccaaa caggaaacaa gtatgaacaa ggaagcttta atcaagctct tgggcttggc
     7441 ggcggatgcc gacgacaaag ccatcgaagc ggctttggcc gaagcacagg aaaagctggg
     7501 cggtaaaacg cttgccgaag cactggccgc acccaaagaa gaaccgcaag gtagcgaagg
     7561 cgataaaggc gatgccggca aacccgaaga caagccgcaa ggcggcaatg ccgacgacgg
     7621 cgaggtagcc gaactcaaag cgcaagtggc cgcgttgagc aaaaaagtga ttgcaatgga
     7681 agtgggcggt acttcagacg gcctgatccg tgccgcgctt tcagacggcc gcctgttgcc
     7741 gcatcaagaa gcatcggcgc gccaactggc cgccaaagac cctgaagcgt ttaaagccct
     7801 gattgacggc agcttggcac tggccgcttt gagcaaaacg cagaccggcg gcaaaggcgg
     7861 cgcagacggt acgtccgcgc tgaccgccga agaagcagcc gtcgccgcgc aattgggcat
     7921 ctctgccgaa gattatgcga aggccaagta atccgataaa ggaaaagaca acatgattat
     7981 cacaccagac accttaaaag ccctgttcac cggctttaaa aagaatttcc aagacggcct
     8041 gaaaatggcg gaaagccaat acaaggaaat cgccaccgtc attccgtctt ccactgcgtc
     8101 caatacttac ggctggctcg gccaatggcc cgctttccgc gaatgggtgg gcgaccgcgt
     8161 attccaagat atgaaggcgc acggctatgc catcaccaac aagcattttg aaagctcggt
     8221 caaggtcaac cgcaacgaca tcgaagacga caacgtcggc atttacgcgc cgatgatgac
     8281 cgagatgggc cgtgcttcgg cggtgcatcc cgacgaattg gtgtttgcct tgctgaaaaa
     8341 cgcacacgcc acgttgtgtt acgacggtca gaacttcttt gataccgacc atcccgtgta
     8401 tgaaaaagtg gacggcaccg gtcaagccag taccgtatcc aatattttcg ccggcagcga
     8461 agcggcctgg tatctgctgg acactacccg cgccctgaaa ccgttgattt accaagagcg
     8521 taaagcgaag cagtttaccg ccatgaccgc cgataccgac gaaggcgtat tcatgcgcaa
     8581 cgaataccgc tacggcgtgg acggccgttg caacgtgggc ttgggcttct ggcagatggc
     8641 ggcgaaatcg caagagaaac tggacgctgc cggtttcgag aaagcctaca acgcgatggt
     8701 gagcctgaaa ggcgacggcg gcagaccgct ggccatccgc ccgaatgtgc tgctggtacc
     8761 gcctgctttg gaaaatgcgg ccaaagagct ggtggaaggc gaccgcctgg ccaacggcgc
     8821 gtacaacccg aacaaaggca aggcgaaagt catcgtatcg ccttggttgc tgtaacgaac
     8881 aggcgggcac cgcccgccgg aagggataga aaatggcaaa agaaaaaaac ggacaggaag
     8941 ttggcgcgac cgtcgatgtc caaccggaag aggtaaccgt aaccgctgcc ttacaggccg
     9001 agattgaagc cttgagagcc gaattagaca aagccaatgc cgaaattcag gtagcgcagg
     9061 cagagttggc cgccgcgcaa gagcgcaatg ccaaattgga aagactgctt gaagccggcc
     9121 ttgcacccgg cggcatttca gacggcgaaa ctttgacacc tgaagcggaa gccgcctttt
     9181 acaccggcag cggagcaccc gccgcagatg ccgaagtggt ggcgattaag agcaaacacg
     9241 gccatgcgtt tttccgcgcc ggctaccatg tacagccgaa ctggacgttt gtgcgccgtg
     9301 ccgacttcga acccgccgac tttgagcgcc tgattggcga ccgtatggtt gaagcgcgcg
     9361 aagccctgcc gacggacgca tcatgagcta cgccgccgtt gccgatttgg tggcgcgttt
     9421 cggcgaagcc accatcaccg gcctgaccga cttgtcccgc aagggcgcgg tggacgaaac
     9481 cgtcgcacag caggctttgg atgatgccgc cgccgaaatc gacggctatc tgatgaaccg
     9541 ctataccctg cctctgccca agccgctgcg catcttaacc gtgtattgct gcgatattgc
     9601 ggtgtaccgc ctgtgtaccg gcaaacggca gcttaccgaa gacattgtgc accgctatga
     9661 agcggcagtg aaattcctgc aactggtggc ggcggggaaa gtcggcttgg gcgttaccga
     9721 acccgcaggc gaaaagcccg ccgtacaagg caacggcgtg atgtttacca cacaggaaaa
     9781 ggtgttcggt cgtgatagcg tctattgaac aagccatcag gcagcggctt tcagacggcc
     9841 tcggccaaat ggtaacgggt gtctttactt acggcggcga gttcgacggc gaaggtttgg
     9901 cgcaggtggt caaccagttc cccgccgtgt gggtcatgtt tgccggcatc aaagacacca
     9961 tccgccacga tacgcgcggc agccgtttta aagcgattgg ccaattcacg gtattggtgg
    10021 gagaccgtgc cagcggcagc gaagcagaca gccgtttcgg cggcctgcac cgccatgatg
    10081 tcggcaccta ccgcctgatg cagacctgcc gccacctgct gaccaatcaa tcgctgggct
    10141 tacagataga ccgtctgcaa ccgggggcgg ccaaaagcct gttcagccgc caaatggagc
    10201 aagacgccgt tagtgtgttt gcgctggaat ttgaaacgta ttggtttgaa gacgcgctgc
    10261 aagacggcga ttggccgcgg cctgcggttt cagacggcca acaggcgaaa gtatatgccg
    10321 acgtatccga ataccaaggc cgcaccgaac ccgaacatcc cgactttaaa ggcgtgaacc
    10381 ttgaattgcg tatcccgccg aaaacccccg accaacccgc cgatatggcg gccaccgttg
    10441 aaaccaaggt gaaaccatga acgaaaccat taaagtacgc gccgccgacg gcctgcaagt
    10501 gccgctggcc ggtaaaccac atgaatacat taccgaccaa gaaaccgtta ccgtacccga
    10561 tgccgcctat taccgccgct gtatcgaata cggcgacctt gtggtcgtaa ccgaagacac
    10621 ccaacccgcg aaaggcagca aataatggca tccgcaaaca tcaatttcga aaaaatcccc
    10681 gccagcaccc gcaagccggg cgtgtacgcc gagtggaaca ccaagctggc cgtgcgcaac
    10741 ctgcccacca acaaacagcg cgtgttgatt gtggcgcagc acaacaatcc cgctttgggt
    10801 gagcttaccg agctggaaaa cgtgttttcc gccgccgatg cggccgccaa atacggcgca
    10861 ggcagcatgg cgcacctaat ggttaccgcc gcgattaaag cctatgccta tgccgattta
    10921 agcctgatta ccgtagccga caacaaggcc ggagtggccg ccggcggtaa aatcacctta
    10981 agcggtactg ccaataccca aggcgtgttg cgtgtgagca ttgccaatgc tgacacttta
    11041 accatcggca tcggcgccga agacaccgca gccaccgttg ccgccgccgt caaagccgcc
    11101 atcgatgccg tgcccgattt gccggttacc gccaccgttg ccgaagcagt ggtgacgctt
    11161 accgccaaaa acaaaggtac ggccggcaac gccatccgca tcaaaaccag caataccgcc
    11221 gaaggcatta ccgccgccgt taccgccatg acgggcggcg atgccaaccc cgatattgcc
    11281 gccgcgctca atgccgtagt cgccgaaggc caccacatca tcgcctgcgg tatcaacgac
    11341 gaaaccaacc tgctgaaact gcgtgcccat ttggataccg tagccagccc gatggaaaaa
    11401 cgctgggcaa tatgcgtata cggccaaacc ggtacgctgg cgcaagccac caccttggca
    11461 ggccgtctga accacggcca tatcgtcagc gcgtggtatc gcggcacccc cagcctgcct
    11521 tgcgagctgg ccgccgcctt tgccgctgtg atggcgagcg aagaagaccc cgcccgcccg
    11581 ttgaacacgc tggcattaaa cagcatcggc gtgtgcgaaa gcaaagacaa aaccatgcgc
    11641 accgagcagg aaaacgccct ttataacggt gttaccccga ttgaaaccag tccggccggt
    11701 acgcaggcgc aaatcgtgcg cgccatcacc acctacacca aaaccgccaa cggcacggca
    11761 gacgaaagcc tgctggacgt aaccaccgtg cgcaccctga tttatgtaag ccgcgcctgc
    11821 gtcgaccgca tcgccctaag attcccgcgc gacaaattga gcgaccgcac cccgccgcgc
    11881 gtgcgcagcg aactgattga cgtattgatg tgctgcgaag agctggaaat tttggagcgc
    11941 gtggaagaaa acctgccgaa actgattgtg gaacgcgacc tgcaaaacac cggcatgttg
    12001 aactgccgca ttcccagcga tgtcgtcaac ggcctgcatg tagtaggtat ggttgtagat
    12061 ttatatttgt aagaaaggct gaaacatgag taccgaatac gttggcagcg tcatcctcta
    12121 tctgggcgcg aacgaagtcg aagtaaccaa aatcgacgtg aaagacaaca ccggcaaaaa
    12181 gcccgtaaaa accatgaacc gcacccgccg cgtcaaaggc tttacccgcg gcgtcggcca
    12241 atacgacatt acctttaccg ccgtggtgcc gaccgacggc acggcggtgg actgggcaaa
    12301 gatcgacgac gccaaaatca gcctagttcc cgacgtggaa ggcgcgcgcc cgacatcgta
    12361 cctcggcttc tgcgccaccg aagcgggtga cagctatacc gtagataatg agctggtggt
    12421 ggatgtgacc ggttttgcga ttcggaaggt gttggagtaa agattttctt gttaacagaa
    12481 gccgctgaag cggcttctgt tgtttttatt acactgtttt taataaaatt caatttttca
    12541 agtaaaatac ttgaatttta aaaaagcctt tgatattatt tagccacgtt gaatgtggca
    12601 gtaatgctgc aaacacatga aaaggagctt gaacaatggg actgactaaa ttcggcgcag
    12661 ccgttcgtga ggctcgtcgt caaactaaac aaactctgca aacgatggct gctacattag
    12721 gtacttctcc tgcttttttg agtgctattg aaactggtcg tagtaaagtg ccaatggatt
    12781 ttgttaaaaa ggttgaagat ttttttgaaa acttaggcca accaatagac ggtttaaagc
    12841 aaaaagcgat ggtttctaat gagaatgtat cgttaagcgg cttaagcctg caacaacaaa
    12901 tgctggtggc tggttttgcc agttcagact tcagcaaaga gcaattggac aaatttgctg
    12961 aattattaag aaccattcat cataaagatt cacagaaaga aggggatgat gcagcaaact
    13021 aattattcaa tgcgtggtgt gcgtgtggga atgttaagcg aagaaacgat tcagtttttt
    13081 gcaaacgaag ctgccaaatt tttggtagta gataagcgta ctcgtaaaaa aatggatgca
    13141 tttatggaaa tgctggaaga gtatggcatt gtaattgatg ttgttgctga ttctgaatgg
    13201 ctagtcatta ccaacgcaat gtgccataac ggaacgattc tgatgcctaa tagcctctat
    13261 actcgcattt gcaatggcga ggacgaggct atctttattt tctttcatga attggggcat
    13321 ttgcttttag ggcataaagc catgttgcat tatagtgaag ttcttcccac gaaacaggaa
    13381 gattcagaat ggcaagctga tgagtttgca aaatgtattt taagaaacat gggaattaaa
    13441 tatgtaccag aacagttaag cttacgattt taaaaagaga aatgacttgg aatgttggcg
    13501 catcccaagc cattattgtg gtgtactagc atacttccaa tatgctgtac aagtgtagtt
    13561 ctccatccgc taactgcaaa cagatttcaa agagaaatac ttgaaaggga cttgagtata
    13621 tatttttcag atttgtttgt ccacgaaaag cgaagtgatg gccaccattt ttacaattgg
    13681 aggtgccatt atggctatga ctaaggctcc cgttaccccc gacttggtgg acgatacagg
    13741 tgcatacatt tttagaaagt cttttaccac caaaagtggt aagaaaattg tttctaaaaa
    13801 tgggaagcca tttaaaattc ctgtgaaagt ttaggaatat ataagccccg ctaagcgggg
    13861 ttttttcatt actgacgcgt gtttagtcgc ccatctcatc tccttaagtg acaattaagc
    13921 cttgatttat acaaggttta accaacgttt aagggctttt ttatgtctga attttccccc
    13981 gaactcaccc gcgctatcga agattacgaa ttacgcgttt ctcccgacct gaaaaccgtt
    14041 gccggccgtc tgaaatacgg catcggtgtg attgatggcg aaatgcacca tgatttttcc
    14101 atgcaccttt taaccgtgcg cgaggacatg gcgattgatc cgcaacttga aggccagccg
    14161 cggctggttg ccgcctatgc cgcgtcactg gataaattgg gtggtttaac agccgaatct
    14221 ttgacacctg atttgctgct tgatgagatg gctgctgctg actttgacgc gctgtactgg
    14281 gcgcaagagc tgctgcaaaa aaagcggctt tgcccgcacc ccgcgccgac cgttaccgat
    14341 acgccgtctt aaaactcggc cgatacggca tcacgcccgc ccaaatcgac ggcatgaccc
    14401 aacccgaact ggaaggctgg ttaaaacagg ccgacttaat cgagcgcggc cgtgccgcgc
    14461 cggtagtgat gccgtggttt attcctgcga acgtcaaacc atctgccgcc acgggcggtc
    14521 agacccaaac ctttatcagc aagcgtaaga aaaaatgagc cagcatagtg tagaactggt
    14581 tgctaagttt aaggacaacg ccacgcccgg cctccgccgt ttggcggcgg aagcggaaaa
    14641 aacaggcagc cggcagttgc agaaagccgc agcagtcaag ctgaaacatc aagagatgta
    14701 cagcgctacc gcccgtttgg gcatccgtac tgaacaccaa atccgccgtg aaatccaaca
    14761 aacgcaggcg gcttacaacc gtttggccaa aagcggcatg gcttcgcaac gcgaactggc
    14821 gagggctgca cagataacgc gcagccgtgt acgcgagctg actgccgaaa tcaacggcgg
    14881 tgcaagccgt ttgcaacgta tcggttcggg agtccggaca atcgggcgcg gtgtggccgg
    14941 cgtggcggcg ggtgcggcgg caggagctta tgtattggcg cagccggtca accggacgat
    15001 ggactttgac acatcattgc gtcatgccgc caataccatg tatgcaggca agagcatggc
    15061 cgagaagcgc gcaggtatgg aggaaatcaa gaaatcggtc atggatgccg cctatgtcgg
    15121 cggaacgacg cgtgatgccg ctttggaagc catgaataca atggtggcca gcggagcgat
    15181 gagcgacgaa gccgtgaaaa aactgctgcc gaccgttatg aaaaccgcca ccgccgccaa
    15241 tacggaagga aacgatattg ccaacatcgt aaccaaggcg ttgcaggcgg ggtttaaaga
    15301 ggcggatatt ccgtcgctgc ttgaccgtgc gttgcaatcg ggcgcggacg gcggttttga
    15361 attgaaagac atgtcgcgct ggctgccgca gcagttggct gcaatgaaaa acgccggtat
    15421 gggcgcgacg ctggataatt tttccagcct gctcaatgcc aaccaattgt cgtttatgac
    15481 ggcgggcagt accgacgaag ccggcaacaa cttagtcaac ctgctggcaa aaatcaacag
    15541 tcaggatatt gtgaccaaag ccaaaaaaat taccatcaac ggcaaagaag gtttcgattt
    15601 caccgctagc atgaacaaac ggcaggcagc aggcatgaac tcgcttgatg cgctggtgga
    15661 cattgtcgga gaaatcacgg ccaaggataa gaagtccgcc gctctgatga aacagattaa
    15721 tgccgcgcaa ggcgatgaag ccaagctggc tttgctggaa aaccaaaagg ccttggtaga
    15781 cggcactgcc atcgggaagc tggtttccga ccgtcaggca ttgatggcat tgctggcatt
    15841 ggtcaacaac aaacaggaac tcacccgcct gcaagcaggc cagacaaatg cggcgggtgc
    15901 ggtggacggt gcatacggat tcgtcgccga aggttcgggg tttaaaaaat cccaattctc
    15961 cctcgccaaa tcagaagccg aatacggcgc atttgaaaaa ttcagcgacc gcatcggcgg
    16021 cggccggtac aagctctatg tttgggggcg gcggcctgct ttccaaactg ttcggcggaa
    16081 gcagcagaac cttacaggcg ggccgtctga tgccctcggc ctcctcgcta ggccccgtcg
    16141 cgctcggtgc gacaccgttg gccgtaatgg gcggggcaac ccatttggcg gggcagcgcg
    16201 acaaatatgc ggaatggtca aaaccgtttg ctaaattatc gtcttggctg gaaagtatcc
    16261 tgcctgattt ttcagcgggg gcgaaagccg aatatctgcg caaacgtgaa gaactgggcg
    16321 gcaataatgc cccgctcgat agtcctgttt taaaagagag tatggcgcag cttaaccaat
    16381 cggcacaaac caaccagcag gcaagccagc agtatgtgca ggcggcggcg gagaatcagg
    16441 cggcaacggc gcagattacc ggcgcggcgg cacagatgac ggctgctgcc gcacaaatgc
    16501 aggcggcggc aggcaagccg attccgatta ccgttactgt gcaaaacggc aacattatgg
    16561 cctatgtgaa tcaggcagta gaacgcaaca gtaggaaaaa ttaatggctt ggaaagacac
    16621 tttactggat gcgggcttca agggcgttac ctttgacgtc atcgacgaca cgctgcgcgg
    16681 cacccacgcg ctggcggaac acgaataccc gttcgtacag ggcgcggaca tcgaagatac
    16741 gggtgtatct gcgatggata tggccttgac cgccgtattg tggggtgaag attacgaagg
    16801 ccgcctgcaa aacctgctga acgtattgtg cgaaacgggc gcgggcgaat tgatccaccc
    16861 gatttacggc agcgtgccag attgcgtagt ggcggatttc gaggtcgcgc acaacgaaga
    16921 aaaccccgac tactgcaccg tgcggatgac ctttaagcaa agtgtcaaag ccgcgccgtt
    16981 tttcgaccgc gacttgccga tagccttggc cgacgaagtg gatttccttg ccgacttggc
    17041 cgcatggcag ggcttcgagg tgtttcagac ggccttaaac aaaattcaga aagcgcaaag
    17101 ccgctggaat gcttttcatg ccacggtatt aatggtggtg ggcgtattgt atgggcaggt
    17161 aaacggcatc ttcaccggcg gcctgaacct gctgaacagc ccgcgcgtat tggtggcgga
    17221 gctgaaatct gtgtttggtg cgctggccaa tatgcacaat gtcggtaaaa gcgggctgga
    17281 cggctggcgc gacatggtgg gcggcgtatc taaagcggcc gctacgccgt ggcaggtcag
    17341 ccgcggaacc gaaggcagtg tggcggcggt agatttaatc caacgcgcca agcccgaaga
    17401 tgtggctgcc tttaccgcgc ttaccgccac cgttggcgcg tgtgcgctgg ccgaacaagc
    17461 cgccgatatt ttggccgtac aactgaacga gccgactttg acgcctgtgg aaatctcgcg
    17521 tctgctggcc gatacccgtg ccgccttgca gcgtgctttg gccgcccagc gcatcttggc
    17581 catgatgctg gcggatgaaa ccaaagccga caagctggcc tattgcctgc tgcggctgta
    17641 tcaaacaccg gtagacagtg ccgatgatgt gtaccaacgc atcgaagtgg ccgggctgct
    17701 gccgcaagcc ccttatctgg aaactgccgc cgcgcttacc gaaagtctgc gcaatacggc
    17761 gcataaactg caaaaacagg cgtttacagt catcaatatg aagccgccgc tggtacagaa
    17821 aaccgtcacc tgcgatatcg gcctgcacct gctggccttt gagtggtacg gcgactacag
    17881 ccgctttggc gaactcttgc gcctgaatcc gcaaatccgc cacccgaatt ttattgagaa
    17941 aggcacggta ctgaatgcct acgccagata acaccgttac gctgctgatt aacggtaaaa
    18001 cacacggcca atggacgaat tacgacattg tttccgacct gctcaccccc gccgatgact
    18061 tttcagtcat gctcggccgc cccgtcgatg ccaaacccga tgcggtgcgg gcgggcgaca
    18121 aggtggaagt gcgtgtgggc ggcgatacgg tattgagcgg ccgcatagac cgcgtgcaga
    18181 ccgttaccga aaaaggcggc aaaaccttaa ccatacaagg ccgcgacgat gcgggcgtgc
    18241 tattggactg ctccgccccg ctgtttaacg cgcaagatat ggatttaaac caaatcatcg
    18301 aaaaaatcgt caagcctttg ggtttggcca aaatccgcat tgatgccgcc aaaaccgaca
    18361 aaacccacaa agtgcagatc gagccgggca gccgcgcgtg ggacgccctg ctggaatatg
    18421 ccgaggcaaa cggtttgtgg ccgtggctgg agccggacgg cacgctggta gtcggcggtc
    18481 ccgattacac cgccgcaccg gtggccgaac tggttttacg gaccaacggc caaaacaaca
    18541 acatcaagcg tctggaagta aaccgcgata tggcggcacg gtacagcgaa gttaccgtat
    18601 tggcgcaaag ccacagcggc aaaaacaaca tcaaagccac cgccaaagac gaatccgtca
    18661 aactgcaccg ccccctaatc gtcaccgagc cggacatcga cagtcaggct caagcgcagc
    18721 gcaaggcgaa aaagcggctg gccgacagca ggctggaagg cttaacaatt accgccaccg
    18781 tacaaggcca ccgcactgac gacggtacct tgtggcagcc cggtcagcgc atcaacgtat
    18841 tgagcgaacc ggacggcatc gacgcggtgt atttcctgat ggcgagaacc ttcatcggcg
    18901 gtcgcggcca acccaccgaa accgtgctga cactgaaaga ggacggcgca tgggtattgg
    18961 atgccgaccc gccgaagaaa tcgggcaaaa ccaaaaggcc gtctgaaagc cgcaaagcca
    19021 acggccacgc agccgccaaa ccgaaaaaac gccgccaagc caagcaggcc ggacaggaat
    19081 tgcaggtgat ttaaatggat gcaaaaacca tagacaaccg tatccgacgg gcatttaacg
    19141 gcatccgcca agccttccgc ggcaaaatcg cccgcgtcaa agcagccggc ggcgtgcagc
    19201 aaatccaagt ggaaggtttg gaaggcgaaa ccgtgcagga cttggaacac gccgaaaact
    19261 tcggttttac gtcgaacccg cccgcaggca gcgattgcgt cgtcgtaccg ttgggcggca
    19321 aaaccagcca cggcatcatc gtcaccacca ccaacggcgc ataccgcatt accggccttg
    19381 ccgaaggcga aacggcggtt tacaacgccg acggtgccaa gatggtgtta aagaaaggcc
    19441 ggattatcga aattgactgc gaaacgctga atatcaaagc accgggcggc gtgaatattg
    19501 acgcgcccaa tgtaggctgc accgcgcaga ttaccgccga gggtcaaatc aacggcaacg
    19561 gcggcatggc agtgaaaggc ggcagcggca catcatttac cggcaatgtg cagatggtcg
    19621 gcgatttgga cactaccggc aaactgacca acaacggcaa aaatgtcggc tccgaccaca
    19681 aacacaacgg cgacagcggc ggcacgacat ccgacccgct gtaattcaga cggcctgaca
    19741 tccatcaaac caaactccaa gcgtccttac ctgaaaataa aggtatggac gctttactta
    19801 atccccaaac gggcggctat gtggtcaacc aatccgccca atccatcgaa aacgagttgt
    19861 atatccgctt ggtcacgccc ttgggcagtt actgggcaga ccgcacgctc ggcagccgcc
    19921 tgcacgaatt gcgccgccaa aagcatttaa agcgcatcga agtactggcc aaacagtacg
    19981 ccgagcaagc cttgcagccc gtgattcagt ccaaacgcgc ccaatccatc caagtaaccg
    20041 catccgcccc gcagcacggc tggctgaagc tgcatattga agccgtcgat gccgcgggcg
    20101 atacagtaac cctgaaccac aaggtagccg tgatatgacg caggcactga atttagaaca
    20161 aatccgcgcc aactatctgc gcgacctgca aaaccaaaac cctgccgccc atgtacacgc
    20221 gggcagcgac aaccatgtac gcgccaccgc cattgccgca gtgggcgaag gccaatacca
    20281 gcatcaggag tggattttgc gccaagcctt tgccgatacc gcagacagcg cctacctaga
    20341 aaaacatgcc gctaaatacg gcatctaccg aaaaaccgcc accttcgcgg gcggcaaggt
    20401 gcgtgttcgc ggcgcggtcg gcgcaacggt gccggttggc caacagatca atgtgggcga
    20461 taaggtgtat ttaaccgccg aatccgccgt tatcagtgcg cttggaagtg ccgaaatagc
    20521 cgttatcgcc actgttgcgg gtagtgctca aaatcaaacc gccgaaaccg cggctacgct
    20581 gcaaagcgta cctgcgggga ttgacagttc cgccgtctaa cgatggtcgg cggtacagat
    20641 gccgaaagcg atgagagcct gttggcacga tatgaagaac gtctgcgccg acccgctgcg
    20701 ggcggtaatc aatacgactt ccgcaattgg tgcttggaag taccgggtgt ggttgatgcc
    20761 tttatctacc ctttgcgtcg cggcaacggc tttgtcgatg ccgtcatttt gggtgaaaac
    20821 ggtatcccca gcgcagaaac actggccgcc gtacaagctc atgtcgatgc ggtgcgacct
    20881 gttacccgta aaaacggctt tctagccctt gcgcccagta tccagaccgt gaatgtggcc
    20941 gtcaccatta ccttaagcag cggcacggat accgatacgg ccaccgctgc catcaaatca
    21001 gctgtaaacg cctattttga tgccttaaag cccggagacc ccctaattaa aagccagttg
    21061 gaaaccttaa tcagcgaagt gtacggcgtg cgtgaccgtg tcttaaccac ccctgtgggc
    21121 aatatcaagc ctcaggagag tgccgaagac atttactggc tgcgcccggg cagtattaac
    21181 gtggagtaca ccacgtgatc catcaaacct tactcgccgc catgcgtccg cccgtcagct
    21241 acgacaccgt aggagaaacg gcagaaatca aagccgaagc gggtgtgttt gatattgtgg
    21301 ccgaccatgc ggaaggagtg aaaaatgcgc cgttccctga tgcggaaaac gattacctgt
    21361 accgctggga agagctgctc gccatcaccc cgcccgcagg agccaatacc caacaacgta
    21421 ctgatgccgt gctggccaaa ctcaatgcct tgggcggttt gagtattgcc tactttaccg
    21481 ccatcgccga atcggcaggc tacaccgtca ccatttacga agaagaccaa ttccgcgcag
    21541 gtgaaagctg tgcgggtgat tgtttgaata ccgaagacgc catctggcgt tggtgcgtcg
    21601 acatcgccga cggcaaagcc accgcctata ttttccgagc cggtcaaagc cgtgcgggcg
    21661 accgcatcag tgtgtacacc gacccgatta tcgaaacgat gtttgaagaa ttaaaaccgg
    21721 catggacgta ttgccgcttt gaatatgaag aagaggtata aaaatggact taatccaaac
    21781 cccgaataag caatttgtcg acggcgaccg ccgcacgccc ggtactcccg tacccgcatg
    21841 gtggctgaac cagttacaag gcgagttgta cagcatttta aacgcggttg gcattgagcc
    21901 taacaaagcc gaccatgccc aagtcttatc ggccattaaa acgttggccg ccgatgcttc
    21961 gcaggttgcc agtatcgatg ctctgcgtaa atacagcggc acaggctatg tgaacgtcaa
    22021 cgcctatcac gccaatacaa cagtgggcgg cggcgtgttt gtggcggata aagccgataa
    22081 atctaccgct gataacggct gtaccgttat tgtttctacc gacggcacgc gctggaagcg
    22141 tgtgttttca gggatgctta acctgcatga ttttggatat gtggccagca aaaacaatgc
    22201 actatccact ttgaatgccg ctgaatctgc cgcgcttgac gtagttgttg attgcttggg
    22261 tttgtcaatt gatacgggta atacctaccc gcaaaaaaac aaatacacaa acggcaagtt
    22321 tgtgattaac ggcaaaactg tcgatgttca ataccagcct atcagaagcg gtatcggtcg
    22381 attcatctcc ggaactggtg cagcagccaa cctcaaatcg aatgaatgga ctggcgcggg
    22441 tttaatcgtt attggcgaag gcgcaatgga gcagatggag aaatgtgttt cctcaatcgc
    22501 tattggcgac cgtgcgcagg gcttttctaa agtaagcagg gacaacatcg ccattggggc
    22561 cgacagcctg attaatgtgc aggccgctac tgaatggtac gaccagtcac gcatggaagg
    22621 cacgcgcaac atcggtattg gtggtaatgc aggacgcggc atcaccagcg gttactctaa
    22681 tgtgtcaatc gggcgcaatg ccggacaggg attgggtgaa ggctcgtcaa atattgcact
    22741 tggcgcaggc gcgatggctg gtactgctcc agtcggtttt agtggcgaca ttgaagtttt
    22801 ttggccgtct tcgacctcaa gaacaatcgc aatcggcgag gctgtcttgc aaacatatca
    22861 gggccgcgcc gctcaaaccg caattggtgc caatgcggcg cgaaatacaa aaaaggccga
    22921 aaaagttacc gcaatcggtt ctgccgcgat ggagaatctt gagcgaaacc gcgccccaaa
    22981 tggcggagat gttgtctaga cgggaacgga agcaggtacc tacgcccaat ctggaaaaaa
    23041 catcacgctt acatttccca acattcgcgg tgcgcaagcg acttattggg tgggcatccg
    23101 ccttacatca ggcacggcgc aaaccttaca aaacgacgtc gtaccggctc aggtcgtatc
    23161 agtgaatggc aatacattaa tcatccaaag ctcaaaagag ctgaccgcca ccggcgcggc
    23221 cgaactgaaa tacgtttatt ctgtaaattc aaccgctact aaaaacgaag agttgaccat
    23281 catcggcgcg aacgccatga ataaggcatt gaccgcagga tactcaacta tcatcggcgc
    23341 agatgccgcg ttgttgggag acaattatca aaaaacaacc gcaatcggcg catcatcttt
    23401 acgaacaggt agtcatattt ccacaactgc tattgggtat tgggtaatcc ctttggcaag
    23461 tagtgagaaa tgtgttgcca ttggagatag tgcgggctat cggaacgttc aaggcgactt
    23521 tttgactggg aaaataacaa actccatcgc catcggatat ggcgcaagaa taaacggcga
    23581 taacgaaatc caaatcggta cgacagggca aactttatat gctccaaccg cggtgaacat
    23641 ccgttctgac ggccgcgaca aagcagatgt taagccgttg acgaacggtt tagattttgt
    23701 aatgaagctc aagccgatga ctggctacta cgaccgccgg gattcctacg ttgacgaatt
    23761 attcaaagac ttgccggcag atgaacgagc ggacaaagtc cgcgaatggt gggcgaatcc
    23821 aatcaaggac ggcagtcata aagaagatcg gttgcggcat tggtttattg cccaggacat
    23881 tgctgcgctg gaagatgaat atggtcgatt gccgatggta aataaaacaa acgataccta
    23941 caccgtcgaa tacgaaacgt tcatccccgt tttgactaaa gccattcagg aaatggccgc
    24001 aagaattgaa acattagaaa ccgaaatgaa ggaatcgaaa aaatgacaag atgtgtgatt
    24061 gatcaagacg gcttgttcgt agaggaacag tattttgatg acggccgcca aagcatcgaa
    24121 gctgaaatgc ctgatctcgc acaatatcag gccgcccagt gggatggaca aggttggaag
    24181 ataattcccg actatcgcgg atgcgtagtt tttgccggtg ggcaagagca ggtgtgggat
    24241 aagttaggtg atttgcccga tggcgtcagc ctgaccccgc ctgaatcggt aaatattgac
    24301 ggcttaaaat ccgtaaaact cgtcgcatta aatgctgccg ctcaggcttt tattaacaag
    24361 cacgccggta tcgacagcgt acctgaattt gagtttgcaa gctgggcaat tcaagccgct
    24421 gaagcgaagg cttggcagga agataaagcc gcgccaacgc cagtgcttga cggcattgcc
    24481 accgcccgcg gtatgtcagc agacacgctt aaagcagcgg ctttgcgtaa aacgctggct
    24541 tacgaacaac tcgccgcaca tgtggcaggt caacgacaag cgctgcaaag caaaatcgaa
    24601 gcagcgaaaa cgcaggccgc gcttgataag attgcagtcg tattcacact gccggaggcc
    24661 gtctgaatgg ttcaagtcta tttggcactc tataaaggca aagccgcaat caacaccccg
    24721 cgcgatgtgg ttaaacgcat tgccgacagc gttgtacgat tggcaacatg cagcccgtac
    24781 agccattgtg aaatcgctgt taagcaccca cgcgacggcc tgtttgattg ttattcgtct
    24841 agcgcgagag acggcggggt gcgcattaaa accatgccgc tgcctgctga taaatgggac
    24901 ttaatcccgc tgccgcaatc tgttgccata tcggccagcc gcttgttcca ccgtacacac
    24961 ggtgcaggtt acgactggct aggtgcgatt ggcgtggtac tcaaatcacc acacagcaaa
    25021 agccgctggt tttgcagcga atggtgcgca tatgtaatag gctacactaa cccgtgccga
    25081 tacagcccgc aaaccctgta tgccgcggta tcaactaaag ataggccgtc tgagaaaatg
    25141 gaggaaacaa agtaatttag aaagttttaa ataaagagga gcggcgacgt gtctgtgttg
    25201 cgagcaccgg cacgccagcc aagcagatgt accctgcatt gacttcaagg ccgctttgcc
    25261 tagctaggcg gcggtaattc taacctaaac ggagttaatg cgacatggtt tattatcgtg
    25321 aattacgttg tgtctactgc aaaaaaacta ttggccaaag gcagcggtaa cgtacaaatc
    25381 aagtgtaacc gttgtaaaac ggttaatact ttcagctaga caaccattac taagaatgcc
    25441 gtcgagcatc attttaaaac tgatttcaga acaccagcga gagtgtcgga gagtaagtaa
    25501 aaatgatgca aaaataccac tcaacggccc cgctgccatt cgtcggacag aagcgatatt
    25561 tcattaaaca cttcactaaa gtattgtcgc aaattcccgc tgacggcaaa cattggacaa
    25621 ttgtagacgt attcggcggc agcggcctgt tggcgcacgt tgcaaaacgt atcaaaccgc
    25681 aggcgcgggt aatttacaac gactatgaca actactcaga ccgcctgcgg cacatcccag
    25741 attacaaccg tttgcgtgaa caaatcgcgc agatagttgg cggcatcccc aaaggctcaa
    25801 ggctagaccc tgaacgtacc cgatcagtgc aacaaacaat cactaatttc caaggccaca
    25861 ttgatgtgcg cgtactttca tcatggcttt tatttagtgc caaacaggca aattcgcttg
    25921 aacaattgct ggggtttgag ttctacaata aggtacgcca atccccatac tccatcgctg
    25981 ccgactattt agacggcctc gaaatcaccc agcaagacta taatcttttg atggctgagc
    26041 atcagcataa ccccaatact ctgctggtat tagacccgcc ttatgtgtcc actgctcagg
    26101 gtgcgtatgc cgctgataaa tactttaata tggttagctt cctgcgtatg attcagtata
    26161 tgcgcccacc gtttatccta tttagctcca cccgtagcga ggcgctggac tacttccaat
    26221 ttttgcaaga gtgcgaaccg gacaaatacc ggcgcttcag cggctacaat atcgtttcac
    26281 tagatgccaa gatgggcaaa ggaatcgagt atcaggacaa tatgatttat aaaatagatt
    26341 aacccgccta aacggcgggc ggtatcaaag ttccacctta tagcgcggcc taactaggtg
    26401 tcaaagattt tccgaaaagg tgctaaagtt cgcgccgcct tataaactgc acccgttttc
    26461 gggtgcagtt ttttattgcg gattgggacg ggaatggaaa aggatgtgga ttttttattt
    26521 taatcggcta tacaattgct gctttcatgc cgtctgaagc acgtttcaga tggcatcggt
    26581 ttacatcaga taaggataaa aatgggtatt tacgattttc aaatgaaaga tgcagaaggc
    26641 aatgcggttg atttgtcagg ctatcgcggc aaggttctgc tgattgtcaa cacggcaacg
    26701 cgttgcggtc tgaccccgca atacgaggct ttgcagaagc tgtatgcaca atataccgca
    26761 gaaggcttgg agattttgga ttttccgtgc aaccagttcc gcgaacaggc tcccgaaagc
    26821 agcggagaaa ttgcccaagt gtgtatgatg aagtttggca cgaagtttaa gattttcgac
    26881 aaaatcgaag tcaatggagc aaataccgcg cccttgtatg cctacctgaa atccgtcaaa
    26941 ccgcaagata aaggcaatca tctgtttaaa gatttcgtgt tgaagctcgc agctttggga
    27001 gagaagcgcg acgaaggcga catcaaatgg aactttacca aattcctcgt caaccgcgac
    27061 ggcgaagtgg tcgagcgttt tgcgcctagt gttacgcccg aagaaatcga agccgatatt
    27121 cgggcattgc tgtaatctgc cgccgtcttg ggaaatgccg tctgaaggct tcagacggca
    27181 tttgtccgtt tgcgggttta ttccgaaata tcggcaacac cgctttcggg gaagatgggc
    27241 aaatccggcg gtgggagcag ggtatccaaa gtcagggcgg acgcatcgag tgtggggtag
    27301 ccttcaaacg cgaccgaata accgctgttt ccgttcatac ggattcgggc gtgcgcgccc
    27361 agagggaaag tgattttgtc ggcaatgtgc ccgaagggaa agcccgtcag cacggggatt
    27421 ttcgccgtgc gcgaaacatg gttggcaacg gcggaaaaat cataagacgg atcatagaca
    27481 tctcgaattt tttccatacg gaaattgccg aacacgatgg cgcgctgttt cttcaaaata
    27541 cccgaaagat acagcgtatt gagcatacgt tcgatgcggt agggctgttc gccgacatct
    27601 tcgaggaaca aaatgccgcc gtcgatgtcg ggcatataag gcgtgccggc gagcgaggcg
    27661 aggacgctta agttgccgcc ccacaatatg ccttcggttt cgacatcggc gcgttggata
    27721 taaggaacat caacggtcag gcggttttgg gttgcaccct tgataaaggc atccatcgta
    27781 aacgcaccgg gggcgggttt gccaaaatcg ctataagcca tcgggccggc aaaactcatc
    27841 atattgcctt ttgccaacaa tgccagctgg acggcgcata cgtcgctgaa tccgaaaaac
    27901 agcgtgccgt gttcgcgcat ccttgcgccg agcgaagcaa aatcgatatg cggcagaatg
    27961 cgtgccgcac cataaccgcc gcgcaagccc atcagcactt taggtgtggc aacgcgaccg
    28021 gaagcgacat cttggaaatc ggcggcacgt tgtgagtccg tgccggcaaa ccgttggaaa
    28081 cggcggctgc ccgcctgttg gttggttacg gtaaaaccgg cattgtaaag gcgggttaag
    28141 gctgtgttga cgcggttggt gtcttcggca aagcctgacg aagcgacaac gcgcagaagg
    28201 ttgccgtccg aactttgacg gcgtggcgtt tggagaggca cggttcgggc tttcacaacg
    28261 gaatggaaag agggaagggg cggaacggat gtggcggatg tgccgcaagc ctgaagcagt
    28321 cccgcgccga tggcggcggt gcaggttttc aggaaacggc ggcgggaggt cggttcggtc
    28381 ataatttccc tttcatacgg tttcagacgg catcggcggg cttaaagcag ctttttaagc
    28441 tgttccgtcc aatcttgcgg cagggtcata ccgtgtttcc ggacggggtt tgcccaaatc
    28501 ggtgcaggga aagacgcatc gttttcaaag cgggcgataa tatgccaatg caggtgcggc
    28561 acgacattgc ccaagctggc gaggttaatc ttggcaggtt gaaacacttg gcgcatagcg
    28621 gcttcgactt tgtacaccat ttccatcaat tcgccgcgtt ccgctgccga aaggtcggtc
    28681 atttcggcaa tatgcttacg ccaaatgacg cggcagaatg caggcgaacc gctatcatta
    28741 tggacggcga tgacgcgcag cttgggggtt tgcagcaaaa cgtcttcatt ttgcgccgtg
    28801 cagatggggc agggttgggc ggtcattttt ccttattcct gaaagtgttt aaaaaacggt
    28861 agggtaattt ctgatatagc ggattaacaa aaatcaggac aaggcaacga agccgcagac
    28921 agtacaaata gtacagaacc gattcacttg gtgcttcagc accttagaga atcgttctct
    28981 ttgagctaag gcgaggcaac gccgtactgg tttaaattta atccactata gtttaatttg
    29041 tctgtccgca ttgccgctaa ttgtaccact tcatcgtatt tccttttcgg ttgaaacccc
    29101 gccctttagg gcggcaggat cagactttat ttgggagggg gtgtaacccc ttccaaatca
    29161 ggacgacaca tagggaggtg ctttatgtgt cgtcctgtgt gttgaaacat atcggacgcg
    29221 tttgaacagt tgtgcggcaa actgttttag cggcaggcaa gagatgccgt ctgaagccca
    29281 ttgtccgtca ttcccacaca ggcagttgct acggttgctg aagcccctta gccgtcgcgc
    29341 aggcggttgc tacggttact aaaatcccgt cattcccgcg caggcgggaa tccagtccgt
    29401 tcggtttcag ttgtttctga taaatcttgc agcgttgg
//
LOCUS       DAWFLT010000020        26463 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWFLT010000020 DAWFLT010000000
VERSION     DAWFLT010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26463
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.020"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(242..844)
                     /locus_tag="WGR74_000874"
     CDS             complement(242..844)
                     /locus_tag="WGR74_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ0905325.1"
                     /translation="MTDVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            complement(878..2149)
                     /gene="purD"
                     /locus_tag="WGR74_000875"
     CDS             complement(878..2149)
                     /gene="purD"
                     /locus_tag="WGR74_000875"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ0905326.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVASGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARIVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPHTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            2307..2624
                     /locus_tag="WGR74_000876"
     CDS             2307..2624
                     /locus_tag="WGR74_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ0905327.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKHAVALIRDMDNFYT
                     HEYQACHRAIEDGAEVDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            complement(2676..3287)
                     /locus_tag="WGR74_000877"
     CDS             complement(2676..3287)
                     /locus_tag="WGR74_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ0905328.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIVFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     VLERDLQLDCRLTVSLSLVNYEACVAAWKSTRQIEVNTEKPFE"
     gene            complement(3350..4285)
                     /locus_tag="WGR74_000878"
     CDS             complement(3350..4285)
                     /locus_tag="WGR74_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ0905329.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKVDLLVAGSGASAVV
                     ESAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTIFVRPIYAGNAFETVQVDSEKLVLTFRATAFDAAPVQGGH
                     AEVINVEAIPAQNLSRFVNRKLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEVLKN"
     gene            complement(4296..5045)
                     /locus_tag="WGR74_000879"
     CDS             complement(4296..5045)
                     /locus_tag="WGR74_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ0905330.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVHIEGGEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLAPADLATDILPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            complement(5272..6240)
                     /gene="waaC"
                     /locus_tag="WGR74_000880"
     CDS             complement(5272..6240)
                     /gene="waaC"
                     /locus_tag="WGR74_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ0905331.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFQAETWREMGHLKQTLRQEAFNFVLDSQGLIKSACF
                     AKMAKSPIYGLDKNSAREGLAALAYAKTYAVPKGKNAVWRNRELFAQVFGYAMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKMNLLQAAYLLKHAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            complement(6277..6912)
                     /locus_tag="WGR74_000881"
     CDS             complement(6277..6912)
                     /locus_tag="WGR74_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ0905332.1"
                     /translation="MIVSIDVDAQKTFTPLCPKELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDMVIVGGLATDYCVKTTVLQL
                     LKGSNWQVIVNEAACRGIAPDTIETAWQEMRSAGAVILKNAEEIKKYINNQ"
     gene            7027..7707
                     /gene="tsaA"
                     /locus_tag="WGR74_000882"
     CDS             7027..7707
                     /gene="tsaA"
                     /locus_tag="WGR74_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ0905333.1"
                     /translation="MTYTIVPIATARSPYKQKFGIARQPGLVSAAEVCIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLNRIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEIRFQIEENRATV
                     IDLSPTPL"
     gene            7776..7895
                     /locus_tag="WGR74_000883"
                     /pseudo
     CDS             7776..7895
                     /locus_tag="WGR74_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7965..8969)
                     /gene="gap"
                     /locus_tag="WGR74_000884"
     CDS             complement(7965..8969)
                     /gene="gap"
                     /locus_tag="WGR74_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0905334.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            9411..12005
                     /gene="mutS"
                     /locus_tag="WGR74_000885"
     CDS             9411..12005
                     /gene="mutS"
                     /locus_tag="WGR74_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ0905335.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKNIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSANGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTTLPQLQKAAKAAAALDVLSTFSALAKERNFARPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPFDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNELENQAAANRP
                     QLDIFSTMPSEKGDEPNVDSFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            complement(12129..12620)
                     /locus_tag="WGR74_000886"
     CDS             complement(12129..12620)
                     /locus_tag="WGR74_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0905336.1"
                     /translation="MNSLFVDNTVFITRLKAVHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDGELPLAFAAVDDSGILLGSATVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWVVVGQHFHNGEWVTVMRLDV
                     DKV"
     gene            complement(12617..13018)
                     /locus_tag="WGR74_000887"
     CDS             complement(12617..13018)
                     /locus_tag="WGR74_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905337.1"
                     /translation="MPSDVGMRLQTAFKRKLNMKKILYFLMFVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGERRRRGIDR"
     gene            complement(13039..14433)
                     /locus_tag="WGR74_000888"
     CDS             complement(13039..14433)
                     /locus_tag="WGR74_000888"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ0905338.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMEWVGLDYDNADNVVYQTRRFDRYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGATLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNGKLAELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNTLADECLYFYVKQTPTEADVQKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            complement(14549..15205)
                     /locus_tag="WGR74_000889"
     CDS             complement(14549..15205)
                     /locus_tag="WGR74_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ0905339.1"
                     /translation="MKKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            complement(15202..15555)
                     /gene="arsC"
                     /locus_tag="WGR74_000890"
     CDS             complement(15202..15555)
                     /gene="arsC"
                     /locus_tag="WGR74_000890"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ0905340.1"
                     /translation="MSEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLESARGMMRVKDDLYKELGLDNPDLDNNALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            15679..16161
                     /locus_tag="WGR74_000891"
     CDS             15679..16161
                     /locus_tag="WGR74_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0905341.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKSYGNNVGVLPFTVVEAPKCGYKQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            16169..16360
                     /locus_tag="WGR74_000892"
     CDS             16169..16360
                     /locus_tag="WGR74_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905342.1"
                     /translation="MPSESASDGIFPARPSVSAKLIHYLKTGGIFIIGTVLPIVQTAY
                     PCGRNRPKRYAAHSLQDLL"
     gene            16357..17007
                     /gene="ftsE"
                     /locus_tag="WGR74_000893"
     CDS             16357..17007
                     /gene="ftsE"
                     /locus_tag="WGR74_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ0905343.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKVHFNNQDLGTLTDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            17004..17921
                     /gene="ftsX"
                     /locus_tag="WGR74_000894"
     CDS             17004..17921
                     /gene="ftsX"
                     /locus_tag="WGR74_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ0905344.1"
                     /translation="MSIIHYASLHIESARTALKQLLRQPFGTLLTLLMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLARDKRLDNIRFIGKEDG
                     LEELQSNLDQNLISMLDDNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESANMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPGEIV
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            18022..18270
                     /locus_tag="WGR74_000895"
     CDS             18022..18270
                     /locus_tag="WGR74_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0905345.1"
                     /translation="MLTPEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWNAKAQ"
     gene            complement(18338..19516)
                     /locus_tag="WGR74_000896"
     CDS             complement(18338..19516)
                     /locus_tag="WGR74_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ0905346.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     QYCMDNGASVIVMIHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMADKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDDMILDIGPKSAAELAELLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGE
                     "
     gene            complement(19597..20850)
                     /gene="murA"
                     /locus_tag="WGR74_000897"
     CDS             complement(19597..20850)
                     /gene="murA"
                     /locus_tag="WGR74_000897"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ0905347.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            complement(21070..21145)
                     /locus_tag="WGR74_000898"
     tRNA            complement(21070..21145)
                     /locus_tag="WGR74_000898"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21110..21112),aa:Lys,seq:ttt)
     gene            complement(21214..22188)
                     /locus_tag="WGR74_000899"
     CDS             complement(21214..22188)
                     /locus_tag="WGR74_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0905348.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNSSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFKLAGNPGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEGDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQSDK"
     gene            complement(22221..22670)
                     /locus_tag="WGR74_000900"
     CDS             complement(22221..22670)
                     /locus_tag="WGR74_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ0905349.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHTVVFTHMLLIVFLAKAMFYISW"
     gene            complement(22711..23982)
                     /gene="waaA"
                     /locus_tag="WGR74_000901"
     CDS             complement(22711..23982)
                     /gene="waaA"
                     /locus_tag="WGR74_000901"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ0905350.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHCPMFGILMETEIWPNLMKECRRAGVPLFLANARLSEKSLS
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVRVESADAWREAVEKTLSSEGGGMQMQ
                     AHVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            complement(24045..25493)
                     /gene="gnd"
                     /locus_tag="WGR74_000902"
     CDS             complement(24045..25493)
                     /gene="gnd"
                     /locus_tag="WGR74_000902"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ0905351.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLDEEDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FTAWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGTDPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            complement(25573..25994)
                     /locus_tag="WGR74_000903"
                     /pseudo
     CDS             complement(25573..25994)
                     /locus_tag="WGR74_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696392.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         6604 a         7432 c         6067 g         6360 t
ORIGIN      
        1 aatcatcttc ggcgttttca tttttatgga ttaaaacaac acgggaaaaa tctgttttca
       61 gatgcttgcc cgcttgattg ttcggattat tgtccggaac gacaaaaccg tcctcaaaat
      121 taaagcagac gttgcgtcct tctaccttta tctctgtgca ataacaatca tgtagagaaa
      181 tgctatccga aaaatttttt ctttgtgtat gcaaaaaaag ttttcattca agtacccata
      241 tctaacgcaa acgtttacct gtttccccgt caataatctg actcggcgat ttctgcctgc
      301 cgattctccc accaacaatc cacacatcgc gtccgaattg ccttctgact tccctctccg
      361 tccgacacgc gcgtttgcct gcgcggttgc acgaagtcga gaccaaaggc gtttgcaaag
      421 cctgacacaa gcggcgcgca cctacatggg cgggaaccct gaccgccaac ttgctgcgct
      481 gtttcccgcg caactcaggt aagacacagg atttggcgga taataggaac gttttagggg
      541 cgggccattc ttttctaagc atatcctgca gattttcaga cggcatttga agtaaaggct
      601 gcaattgctc aaattgattc ccgatgacaa tcataccctt gtgttgcggt ctttttttca
      661 aatgcgccaa cttaccgagt gctttggcta atgtcggaag acaccccaag ccataacaag
      721 attcggtcgg ataagcgacc aaaccgcctt ttttcaaata aacgcttaac ttacgttgcg
      781 ctgatgctgc gataattctc ggaaataaca taatataaaa taccgtctga agcacatcag
      841 tcatacttgg cttcagacgg catcatcctc tttctaatta acggttaatc gctttatcgg
      901 caatgtcttt acggtattgc atcccgtcga aactgatttt ttccaacgcg ccatatgcct
      961 tagctttcgc ttgcgccaca ttatcgccca atcccacaac acacaatacg cgtccgccgt
     1021 tggtcaatac gtcacctttc tcgtttgccg ttgtacctgc atggaaaact ttgcctactt
     1081 ggttggcagc atccagaccg gaaataacat cgcctttttt gggcgtttcg gggtaatttt
     1141 gcgccgccag taccacgccc acggcagttt gcgggctcca ttccgcggtt acgctatcga
     1201 gtttgccgtc tattgccgct tcaaccaaat ccgataagtc gctgttcagt cggctcataa
     1261 tcggctgggt ttcaggatcg ccgaaacggc agttaaactc aatcgtatgg ggtgcaccgc
     1321 tttgatcaat catcaaacct gcgtacagga aaccggtgaa ctcatgcccc tccgctttca
     1381 tccctgctac ggtcggcaaa ataatttcat tcatcgcgcg ttcgtacaca acaggcgtta
     1441 ccacaggcgc agggctgtac gcacccatac cgcccgtatt cagacctttg tcgccgtcta
     1501 aaagacgctt gtggtcttgg ctggttgcca taggcagtac attattgcca tcaaccatga
     1561 cgataaaact cgcttcttcg ccttgcagga aatcttcaat cacaatacgc gcgccggcat
     1621 tgcccatttt gttgtccagc agcatatcat caatcgcagc atgcgcttca tccaaagtca
     1681 tcgccacaat cacgccttta cctgccgcca aaccatcggc tttgataacg ataggcgcac
     1741 ctttctgatt gacgtaatca tgtgcggcat cggcgttttc aaaggtttga tattgcgcgg
     1801 tcggaatatt gtatttcgcc ataaatgctt tggcgaaatc tttggaactt tccaactgcg
     1861 ccgcatattg tgtcggaccg aatattttta gtcctgcagc acggaaatca tccacaacac
     1921 ctgccgccaa aggcgcttca gggccgacga cggtaaaaac aatattttct ttacgacaga
     1981 attcaatcaa atcctgatgc gcagtcaagt cgatgttttg cagcttgggt tcaatcgctg
     2041 taccggcatt accagacgca acaaatactg tttccacttt aggcgactgc gccaatttcc
     2101 aagccagcgc gtgttcgcga ccgccattac cgataaccag cagtttcatg ccatctcctt
     2161 gacaaatatg tacttttaac gaaaactcga tacaaaggga cttttatccc atctgaagaa
     2221 attttagtag aatcaaacaa aagaccgctt cattccactc tgcaacctat tcaacttatt
     2281 cataaattaa aaaaggacaa gcaaccatgc aaaaacgtat tgatgaaatc caaagcaaat
     2341 accgcgaatg gtgtcattta ctaccgcaat tggaagaaga catccgccgt tggaaacatg
     2401 ccgtcgcttt gattcgcgat atggacaatt tttataccca cgaatatcag gcttgtcatc
     2461 gggctattga agatggagcg gaggtggatt tgcgcacaga aggcgaatac agcattatga
     2521 gtgaagatgc actatggaac gcgctgggcg aattccatca actggcttgg ttatatttgc
     2581 gctccagcgt cgatgcctta gacaaatata cacaagaaga ttagtcagcg aagaggtcgt
     2641 ctgaaatacc atcacaaagc atttcagacg acctttcatt caaaaggctt ttccgtattt
     2701 acttcaatct gccgtgtact cttccaagcc gcaacacagg cctcataatt taccaacgac
     2761 aaactgaccg tcaatcggca atccaactgc aaatcccgct ccaatacatc cgcctgatat
     2821 tgtttagcaa tgcgtatcgc ttcattcaaa aacggatatt cacatttcag ccaaacagtt
     2881 ttttcaatat tcttttcaac tacttctgca actgccaacg cttgagccgt cgcctctttg
     2941 tacgcatgta tcagacctgg aacacctaac aaagtaccac cgaaatagcg gacgaccacg
     3001 accaaaacgt cggtaatacc caccgaatca atctgtccca aaattggtcg tccagcactt
     3061 cctgatggct ctccatcatc gttggcacga aattgcacac catccacacc caaacgatag
     3121 gcatagcacc agtgtcgtgc tttatgatgc tcttccttta acggatcgag gtattttttc
     3181 acatcagcca atgtccgaat cggataggca aatacaataa aacggctgcc tttatcttta
     3241 aactcagcct gcgtcgagga ggtaatggtt ttataagtcg taatcatgct gaaatgtttt
     3301 cagacgacct cattaataac aaggtcgtct gaacgtttca cgtgaaacat taatttttca
     3361 atacttctgt taattgtgga acgatttcaa ataaatcgcc aaccaatccg taatcggcta
     3421 cattgaaaat cggcgcatca gcatctttat tgattgcaac aatcacctta ctgtcttgca
     3481 taccggcaac gtgttgaatc gcacctgaaa taccgattgc aaaatagagt tgcggcgcaa
     3541 ctactttacc ggtttgtccg acttgagcat cgtttggcgc atattcggca tcaaccgccg
     3601 cacgggatgc accgattgcc gcacctaaaa catccgccaa cggtgtcagc acttcattga
     3661 atttttccgc actacccaac gcacgaccac cggaaacaat cacttttgcc tgagtcagtt
     3721 caggacgatc ggaatgagaa agcttgcggt tcacaaaacg actcaggttt tgagcaggga
     3781 ttgcttcaac attaattacc tcggcatgac caccttgtac aggagctgca tcaaaagccg
     3841 tcgcacggaa ggtcagcacc aatttttccg aatcaacttg cacggtttca aatgcgttcc
     3901 ctgcataaat ggggcgcaca aaaatcgtgt tatccacaat ctcggtcaaa tcagaaattt
     3961 gcggtacatc taataaggct gctacgcgag gcaaaaggtt tttaccgaat gtagttgccg
     4021 ttgctgcaac atagcggtaa tccgctgcca atttaacaac cagcggagcc aactcttcag
     4081 ccaaaccttc ggcataatgg gcagcatctg caaccaaaac tttttccaca cccgccactt
     4141 gttttgcgga ctctactacg gcagatgcac cgcttccggc aaccaataaa tcgactttac
     4201 ccagcttggc ggcagcggta acagcatgca aagtggtagg attcaactgt ttgttgtcgt
     4261 gttcgacaat aatcaatacg ctcatttcag cctcctcaaa tcactttggc ttcgtttttc
     4321 aatttttcaa ccaattcggc aacgcttgct acttttacgc ctgcctgacg cgccttaggt
     4381 tcggcaaatt tcaccgtttt cagacgaggt aaaatatcgg tagccaaatc ggcaggcgcc
     4441 agtttttcca gaggtttttt ctttgccgcc ataatattgg ggagtttgac aaagcgcggc
     4501 tcgttcaaac gcaaatcagc actgataaca gcaggcagtt ttaatgctat ggtttcttcg
     4561 ccgccatcaa tttcgcgcac aatctgtact tcaccacctt caatgtgtac ttttgaagca
     4621 aacgtacctt gcgccgcatt cagcaaagct gccagcattt gcgccacttg attggcatca
     4681 tcatcaatcg cttgtttgcc caaaaagaaa atttgcggat tttctttgtc cgcaacggct
     4741 ttcagcaact tagcaacggc cagagactcc agtttagtat cggtttcaac atgaatggca
     4801 cggtcggcac ccatcgccaa agctgtacgc aaggtttctt cgcatttttt ctcacccaaa
     4861 gaaaccgcta cgatttcgct tacttttccg gcttctttca aacggacagc ttcttccaca
     4921 gcgatttcgt caaacggatt catcgacatt ttgacattgc cgatatccac atccgaacca
     4981 tcggctttta cacgaacttt gacgttgtag tccactacgc gctttactgc gaccagtgct
     5041 ttcattgaac cctcctaaaa agaacgctgc tttcaccatc cagcgaaacc aaaccttctt
     5101 ccctataaaa ccaaatccgt tttccttaaa aacgaattca ttcaaaaatc tttcggataa
     5161 tgcttgccga ttataccatt tttaaagcat ttactcagac tagcggatat acattcctgt
     5221 atctaataaa ttggaaaata tcatgccgcc atatcagttt tagacgaccc tttagccttt
     5281 atctgctgca acacaatcca tcagcgcttg ataaaccaaa tctgcggtcg gaatctgccc
     5341 gatattgccc aaattttttg caattggcga aacctgaacg cctgttttaa tcggatcggt
     5401 atcggtataa atgccgacca caggtttttc caaggcgttt gccaaatgca gcaaaccggt
     5461 atccacgcca acaattccga ccgcgtgttt cagcagatac gctgcctgca ataaattcat
     5521 tttatcgcac acaatagcaa acggcagtcc gtctgcaatt tgtttggcac gcgttttttc
     5581 atcttcattt ccccaaggca ggtaaatatt gcattgctgt tcttcattca acttttgcag
     5641 caacgaccgc cagttttcca caggccataa cttactgtcc cgactggtcg catgcaaagc
     5701 cgcataatac ggctgcgcta aatttttcag acggcctgcc tcaggaacag tcaagccaaa
     5761 tacctgcgtt tcaggcatcg catacccaaa tacttgggcg aacagctcac gattgcgcca
     5821 aacagcattt ttccctttcg gtacagcgta tgttttcgca tacgccagcg cagccagtcc
     5881 ctcgcgcgcg ctatttttat ccaaaccata aataggggat ttcgccattt tagcgaaaca
     5941 cgcgctttta atcagacctt gactgtccaa tacaaaatta aatgcttcct gccgcaaagt
     6001 ctgtttcaga tgacccattt cccgccaagt ttcagcctga aagagatgtt tgcgccattg
     6061 ccgccatttc atcacatgga ttttttttac aaacggatgc aggcgcgcaa tatctgcaaa
     6121 tccagcctca catagccaat gcagttctac atcaggacat tgtcgcgcca aatcttcgat
     6181 tgcgggcaaa gtgtgaatta aatcgcccat actagacaag cggacaagca aaattttcat
     6241 atttaagaag tatgtttcac gtgaaacgat tttaacttat tgattattaa tatatttttt
     6301 aatttcttcg gcgtttttta agatgacagc accggcagaa cgcatttcct gccatgccgt
     6361 ttcaatggta tccggcgcaa taccccgaca agccgcttca ttgacgataa cctgccaatt
     6421 gctacctttg agcaactgca aaaccgttgt tttaacacaa taatccgtag ccaaaccgcc
     6481 gacaatgacc atatccgtat tttgacaacg cagccactca atcaatccag tgctcagttt
     6541 ttcctcaata tcgtgaaaac acgcgccgta aggatgcaat tcaggatcaa cacctttcca
     6601 aacgcaataa tcgtattctt tggcagaagg cagtccgtcc aataattcat agccgcgcgt
     6661 accgaccatc gcatgagcca cccaagtcaa atccgcatca ggcaaacctg tcggcttcaa
     6721 catatcaaca gggttatcca caagccattt cgctaccata tgatgcgcat ctttcgtcat
     6781 cacgcgcaaa tccgccaaag cggcttgggc attcaattct tcgacaatca aatgcccctc
     6841 gtttacaggc agctctttag gacacaaagg cgtaaaagtt ttttgtgcat caacatcaat
     6901 ggaaacaatc atctcatcat ttcaacgcaa ttaaaatgcc ctgtattata acaaattact
     6961 gcccaaaagc ggtaaaacag gctgtgataa gataaggttt ttccgaaaaa cttatccaca
     7021 atcctcatga cttacaccat cgtccctatc gccaccgccc gatcgcccta caaacagaaa
     7081 ttcggcatcg cccgccagcc cggtttagtc tccgccgcag aagtatgcat cgagctgaat
     7141 cccgaattta ccgcagacag cgtgcgcggg ctggaagatt tcgattatgt gtggataagt
     7201 tttatttttc acggcgtatt ggatgaaggc tgggcacaaa tggtgcgccc gccacggctc
     7261 ggcggcaaac aaaaaatggg cgtattcgcc acgcgcagcc cccaccgccc caaccatctc
     7321 ggactatcac tcttaaaact caaccgcatc gaaaccggca aacccgtccg cctttattgc
     7381 agcggcgcag acctgctcga cggcacgccg attgtggaca tcaagcctta tatccccttt
     7441 gtcgaatcca aacccgatgc cgcatctggt ttcgtcagcg gcaaacccgt agagttggaa
     7501 gtcgtttggc aggaaaacat cggcgcggaa aatttatctg caaacaccaa aaaccttatc
     7561 agccaaagca ttgcccaaga tccgcgcccc gcctatcaga atattcccga acggatttat
     7621 gtgatgaata ttgcggatta cgaaatcaga tttcaaatcg aggaaaaccg tgcaaccgtt
     7681 attgatcttt ccccaacccc gctttaaatc gggcaaaaat ccggttttgc cgcatagcag
     7741 ttgaacaaac ggctgttgtt tgttcgccat aagccgcaat atcaagttat agcggattaa
     7801 atttaaatca ggacaaggca acgaagccgc agacagtaca aatagtacgg caaggcgagg
     7861 taacgccgta ctggtttaaa tttaatccac tatacagata aacaatgccg tctgaacgca
     7921 atgtgttcag acggcattta cttatccaca ggtttgttca agccttagat tttgcctgcg
     7981 aagtattcca aagtgcggac gagttggcag gtgtaggaca tttcgttgtc gtaccaggca
     8041 acggttttca ccagttgttt gccgcccacg gtcatcacgc gggtttgggt tgcgtcaaag
     8101 agcgagccgt attcgatgcc gacaacgtcg gaagaaacga tttgatcttc gttgtagccg
     8161 taagattcgc tggcggcggc tttcatcgcg gcgttgattt cttctttggt tacagggcgt
     8221 tcgaggacgg aaaccaattc ggtcagcgag ccggtggcaa cagggacgcg ttgggcggag
     8281 ccgtcgagtt tgccgttcaa ttcggggata accagaccga tggccttggc ggcaccggtg
     8341 ctgttgggca cgatgttgag cgcggcggct cgggcgcggc gcaaatcgcc tttgcggtgc
     8401 ggcgcgtcaa gggtgttttg gtcgccggtg taggcgtgga tggtggtcat cagaccttcg
     8461 actacgccga actctttttg caggactgcc gccatcgggg caaggcagtt ggtggtgcag
     8521 gaagcggcgg aaatgacggt ttcgctgccg tccaaaatgt cttggtttac gccatatacg
     8581 acggttttca catcattgcc gccaggtgcg gaaatcacaa ctttgcgcgc gccggcacgg
     8641 atgtgggctt cggctttggt tttgttggta aagaagccgg tacattcgag gacgacatcc
     8701 acacccaact cgccccaagg caattcttcg ggattcggat tggcaaaaac tttgatttct
     8761 ttgccgttta ccacgatggc atcgtctttt aattcggcag taccttggaa acggccttgc
     8821 gtgctgtcgt atttgaaaag gtgcagcagc atttcggcag gggtcaggtc gttgacggcg
     8881 acgacttcga tgtcgtgggc tttttcaatt tgacgcaatg cgaggcggcc gatgcggccg
     8941 aaaccgttaa tcgctacttt aatgctcata ttttgtctcc aggttgtgaa ataaagtccg
     9001 atgcctgcat tgtattctga aataaagtta cattccacta ttacatctaa ctacttgacg
     9061 cttatttgat atagatgaat tttgctgttt gcacagattt ccaaaacctt taccatcaat
     9121 atttgaattt aaattttaat gatgattttg atgattgcca acctgcttgt gcgtaagtag
     9181 caaatatcca atattttcat tatctttttg tcaaataagt ttgagtttaa gacttgctgt
     9241 ataagacaga taagcgtgga tattttttga cttaataata tttctgtgga taactttgct
     9301 gttttcctag ttgtctccac aaccttattg acaggcttac ggtcagtctc attccgtcga
     9361 agacaaaacc ttttgctaca ataccgtttt cctaatgata aggcagcccc atgtccaaat
     9421 ccgccgtttc cccaatgatg cagcaatacc tcggcatcaa agcgcaacat accgacaaac
     9481 tggtgtttta ccgtatgggc gatttttacg agatgttttt cgacgatgcg gtagaagcgg
     9541 caaaactttt ggatattacc ctgaccacgc gcggacaggt ggatggcgag ccggtcaaaa
     9601 tggcaggcgt gccgtttcac gccgccgaac aatatctggc gcgcctggtc aagttgggca
     9661 aaagcgtggc gatttgcgaa caggtcggcg aagtcggcgc gggcaaaggg cctgtggagc
     9721 gcaaagtcgt gcgcatcgta acgcccggca cgctgaccga ttccgcattg ctggaagaca
     9781 aggaaaccaa ccgtatcgtt gccgtgtccc ccgacaaaaa atacatcggt ttggcgtggg
     9841 catcgctgca aagcggcgaa ttcaaaacca agctgacaac tgtggataaa ttggacgacg
     9901 aactggcgcg cctgcaggcg gcggaaattc tgttgccgga cagtaaaaac gcaccgcaac
     9961 ttcagacggc atcgggtgtt acgcgcctga acgcgtggca gtttgccgcc gacgcggggg
    10021 aaaaactgct gacggaatat ttcggctgcc aggatttgcg cggcttcggt ttggacggca
    10081 aagaacacgc cgttgcgatt ggcgcggcag gtgcgctgtt gaactatatc cgcctgacgc
    10141 aaaacctgat gccgcaacat ttggacggcc tgtcgctcga aaccgacagc caatatatcg
    10201 gcatggacgc cgccacgcgc cgcaatctcg aaatcacgca aaccctctcc ggcaaaaaat
    10261 cgccgaccct gatgtccacg ctcgaccttt gcgctaccca tatgggcagc cgcctcttgg
    10321 ctctctggct gcaccaccct ttacgcaacc gcgcccacat ccgagcgcgc caagaagccg
    10381 ttgccgcgct ggaaagccaa tacaaacccc tccaatgccg tctgaaaaac attgcggaca
    10441 ttgaacgcat tgctgcccgc atcgccgtcg gcaacgcccg cccgcgcgat ttggcatctt
    10501 tacgcgacag tctgttcgag ttggcacaaa tcgacttgtc cgcaaacggc agcagcctgt
    10561 tggaaaccct caaagccgtt ttcccggaaa acctatccac agccgaacag ctccgccaag
    10621 ccattttgcc cgaaccttcc gtctggctga aagacggcaa tgtcatcaac cacggttttc
    10681 atcccgaact ggacgaattg cgccgcattc aaaaccatgg cgacgaattt ttgctggatt
    10741 tggaagccaa ggaacgcgaa cgtaccggtt tgtccacact caaagtcgag ttcaaccgcg
    10801 ttcacggctt ttacattgaa ttgtccaaaa cccaagccga acaagcacct gccgactacc
    10861 aacgccggca aaccctcaaa aacgccgaac gcttcatcac gccggagttg aaagcctttg
    10921 aagacaaagt gctgactgct caagagcaag ccctcgcctt agaaaaacaa ctctttgacg
    10981 gcgtattgaa aaaccttcag acgacattgc cgcagcttca aaaagccgcc aaagccgccg
    11041 ccgcgctgga cgtattgtcc acattttcag ccttggcaaa agagcggaac ttcgcccgcc
    11101 ccgagtttgc cgactatccg gttatccaca tcgaaaacgg ccgccatccc gttgtcgaac
    11161 agcaggtacg ccacttcacc gccaaccaca ccgaccttga ccacaaacac cgcctcatgc
    11221 tgctcaccgg ccccaatatg ggcggcaaat ccacctacat gcgccaagtc gcgctgattg
    11281 ttttattggc acacaccggc tgttttgtgc ctgccgatgc cgccacaatc gggcccttcg
    11341 atcaaatctt cacccgcatc ggcgcatcgg acgacctcgc ctccaaccgc tccactttca
    11401 tggtcgaaat gagcgaaacc gcctacatcc tgcatcacgc caccgaacaa agccttgttt
    11461 taatggacga agtcggacgt ggtacttcca ctttcgacgg cctcgccctc gcgcacgccg
    11521 ttgctgaaca cctgctgcaa aaaaacaaat ccttcagcct gtttgccacc cactatttcg
    11581 agctgaccta cctgcccgaa gcccacgccg ccgccgtcaa tatgcacctt tccgcgctcg
    11641 aacagggaca ggacatcgtt ttcctgcacc aaatccaacc gggtcccgcc ggtaaaagct
    11701 acggcattgc cgtcgccaaa ctcgccggcc tgcctgtacg cgcattgaaa tccgcccaaa
    11761 agcatttgaa cgaactggaa aaccaagccg ccgcgaaccg tccccaactg gatattttca
    11821 gtaccatgcc gtctgaaaaa ggagatgaac cgaatgtgga cagctttgtg gacaaagcag
    11881 aggaaaaaca ttttgaaggt atattggcag cagccctgga gaaactcgat cccgacagcc
    11941 tgaccccgcg cgaagcattg tcagaactgt accgtctgaa agatttgtgc aaatccgtat
    12001 cttaatttcc gttgtcggaa cagcatcaaa ccatatggaa aaatctgtgg ataaacatta
    12061 tctgacagga aatttccaaa tataaaaaat gccgtccgaa cagctcagac ggcatctgtc
    12121 cattcggctt aaaccttatc cacatccaaa cgcataaccg taacccattc accgttatgg
    12181 aaatgttgcc cgacaaccac ccagccgaat gattcataaa atatttgcac atcaggcgta
    12241 taaagataca agaactttat ccccagcgaa cgcgctgcgc ctatgcagtg ggcgaccagc
    12301 ctcctgccaa tgccttttcc gcgatattca ggtaaaacaa agacatcacc caaccaatat
    12361 tcatactgtg gaaaactttc catatcatgc cgcttgaccg tagccgaacc caacaggatt
    12421 ccggagtcat ccacagccgc aaatgccagt ggcagttcgc catccttcaa acacctgccg
    12481 taataggcat gaatcttatc cacagaagac cacggttcaa atccgtgcca ctcctcaaac
    12541 aacgcctgaa ccaacctgcc gatatgcacg gctttcagcc gtgtaatgaa aacagtattg
    12601 tctacaaaga gggaattcat cggtcaattc cccgacgcct tcgttcccct gcgccgtaaa
    12661 ccgcattcca agcatggtcc aaacgcactc cgatttgcct cagctcttca gcctgccggg
    12721 ctttttgcgc cattgctgca ggaatttccg cttccaaacg ggcgatgtct gcctgagccg
    12781 cctgcaaacg ccggcgcgca tcttccaaat ccgactgcat cccgatgatt tttccgtcca
    12841 gattgttttg cttttgcaat aaggcacggt aaccggattg gatactgagc aaattgtctt
    12901 cagcatcccc tgcccatacg cttgtagaaa aaacaaacat cagaaaatac aatatttttt
    12961 tcatgttcaa cttccgtttg aatgccgtct gaagccgcat tccgacatca gacggcatta
    13021 cccgaacctg tggataactt aagcgcggat gcgtttcaac acttcttctt tgccgattaa
    13081 tgccaacaca gcatcgacgc tgggggtttt cgccgtaccg cagacggcaa ggcgcagggg
    13141 catgccgagt ttgcccattt taatgccttc ttcgtcgcag aagggtttga agaggtcgtg
    13201 gatggcttcg gcattccagt cttccagccc ttcgaggcgt tcggcaaagc gcagcatacg
    13261 ggcggcggct tcatcgtccc agtgtttctg cacgtccgct tcagtaggcg tttgtttgac
    13321 gtagaagtag aggcactcgt cggcaagtgt gttcaggtct tgggcgcggt ctttgaccag
    13381 tgccaacacg tcttccaatg caggtttttc agtttcatgg atatcgcgca gagcgagacg
    13441 cggtttgacg agttcggcga gtttgccgtt gggcgtgatt ttgatgtgtt cgccgttaat
    13501 ccagtagagt ttcttcaaat ccatgcggct tggagatgga gaaacgtctt tcaaatcaaa
    13561 ccattcgata aattgttcca tcgtgaagaa ctcatcgtcg ccgtgcgccc agcccaaacg
    13621 tgccagatag ttgagcatgg cttcgggcag gatgcccatc gcgccgaaat cggtaatggc
    13681 aacggtatcg ccgctgcgtt tggagatttt tttgccttgt tcgttaagaa tcatcggcag
    13741 gtggccgtat tcaggcaggg tagcgccgat ggcttttaag atgttgattt gtttcggggt
    13801 gttgttcaca tggtcgtcgc cccggataac gtgggtaacg cccatgtcgt agtcgtccac
    13861 gacaacgcag aagttgtagg tcggcgtgcc gtcggcgcgg gcgataatca ggtcgtcgag
    13921 tgcttcgttg gggatggaga tttcgccttt gaccaagtcc gtccacttgg ttacgccatc
    13981 caaaggcgtt ttgaagcgga cgacgggttg cacgccggca gggatttcgg gcagggtttt
    14041 gcctgcttcc ggccgccagc ggcggtcgta agtcgccgtg ccttcttttt cggctttctc
    14101 gcgcatcgct tccagctctt ctttgctgca atagcagtag taggcatcgc cttttgccaa
    14161 gagttcggcg atgacttctt tataacggtc gaagcggcgg gtttggtaca ccacgttgtc
    14221 ggcgttgtcg taatcgagac cgacccattc cataccgtca aggatgatat tgacagattc
    14281 ggcggtggaa cgcgccaagt cggtgtcttc gatacgcaat aaaaattcgc ctttatgatg
    14341 gcgggcaaac gcccacgaaa acaaggcggt gcgaacgccg ccgatgtgca ggtagccggt
    14401 agggctgggg gcgaaacggg ttttgacggt catgatggct ccagaatctt taaaacggct
    14461 tattttactg tttttaccgt gcttgggcat taaaaatgcc gtccgaaccc tgcctgcgga
    14521 taagtttcag acggcatttt ctaccgcttc aatcagacag ccatcccaaa aagcggctgc
    14581 cccatttttc caaacggtag agggataacg catatccctc ttgcagcata aagatttttt
    14641 tcttatttcc cgcatcaaag cgcgtggtcg gcgtggcaga catataaacg cggacaccca
    14701 aatcctccgc catttccgcc gcccgcgcca aatggtaggg atcgctgaca atcaccacac
    14761 tggcaatacc gttggcacgc aaaaccggac ggatgttgtt caggttttca taagtgttgc
    14821 gcgaagtgtt ttcaaacagg atgttgcgcg ccggaacccc ctgtttgagc gcgtaccgcc
    14881 gcccgacctc ggcttcggtc atatagcctt ttttggtccg gcctcccgta aacacgattt
    14941 tgcctaccct gcggctttgg taaagcgcga tggcgtggtt gatgcgttcc cgaaaaaccg
    15001 gggaagggcg tttgtcccat gcggcggcac ccaacaccag cgcggcatcc gcccggacat
    15061 acggcggcaa aacctgcccg cccgtccgat aaaccgccca aacggatgag gcaaacacca
    15121 gcaaaagcgg aaaaacactc aaacagaaac cgcccaacag gtaatagcgc aagccgttgc
    15181 ggctgcaaaa cagccgtttt ttcacaatac cgcttcgata ttttccaacg gcctgccgac
    15241 agccgcctta ccgtttgcca aaacaatcgg acgctccaac agggcgggat gatcggcgat
    15301 ggcacgcagc agtgcgttat tgtccaaatc ggggttgtcc aaacccaatt ccttgtacaa
    15361 atcatctttc acacgcatca tcccgcgcgc cgactccaag cccaatttgt tgaaaatatc
    15421 cttcaattcg gacaagtcgg gcggcgtatc caaatatttg accacttcgg cagcaatgcc
    15481 gcgttcttcc aatagggaca aggcggcacg cgatttgctg caacgcggat tgtggaaaat
    15541 tttgatttca gacatgacat ttccttactt ctcgacaatc cccttattat cggcttacac
    15601 agggttttac tcaatatccc gcctacaacc gtaccaaacg gtttacaata cccgaatcga
    15661 catacaaagg acaaaacgat gaaatacttg aatcttgccg caatcaccct tgccgccaca
    15721 tttgccgcac ataccgcctc ggcagacgaa ctggccggat ggaaagacaa caccccgcaa
    15781 agcctgcaat cactcaaagc ccccgtacgc atcgtcaacc tttgggcaac ctggtgcggc
    15841 ccgtgccgaa aagagatgcc tgccatgtcc aaatggtaca aagcgcagaa aaaaggcagt
    15901 gtcgatatgg tcggcatcgc gctcgacaca tccgacaata tcggcaactt cctcaagcaa
    15961 acccctgttt cctacccgat ttggcgttac accggggcga acagccgaaa ctttatgaaa
    16021 tcctacggaa acaatgtcgg cgtactgccc tttaccgtcg tcgaagcccc gaaatgcgga
    16081 tacaaacaaa ccattaccgg ggaagtgaac gaaaaaagcc tgaccgaagc cgtcaaactc
    16141 gcccattcaa aatgccgtta aacgccggat gccgtctgaa tccgcttcag acggcatttt
    16201 tcccgcccga ccttcggtat ccgccaaact tatccactat ctaaaaacag gcggaatctt
    16261 tataatcggc actgtcttac ctattgttca gacggcatat ccctgcggac gcaaccgccc
    16321 gaaacgatat gccgcccatt ccttacagga cctcctatga tccgtttcga acaagtttcc
    16381 aaaacctatc ccggcggttt tgaagccctg aaaaacgtca gcttccaaat caacaaaggc
    16441 gagatgattt ttatcgcagg acactccggt tcaggcaaat ccaccgtcct caagctgatt
    16501 tcgggcatta ccaaaccaag caggggcaaa gtccacttca acaaccaaga cctcggcaca
    16561 ctgaccgaca accaaatcgg ctttatgcgc caacacatcg gcatcgtgtt ccaagaccac
    16621 aaaatcctct acgaccgcaa cgtcctgcaa aacgtcatcc tgccgcttcg gattatcggc
    16681 tatccgccgc gcaaagccga agaacgcgcc cgcatcgcca tcgaaaaagt cggcctgaaa
    16741 ggacgagaat tggacgatcc cgtaaccctc tccggcggtg aacaacaacg cctgtgcatc
    16801 gcccgcgccg tcgttcacca gcccggcctg ctgattgccg acgaaccctc cgccaacctc
    16861 gaccgcgcct acgcgctcga catcatggaa ttgttcaaaa ccttccacga agcgggaaca
    16921 accgtcatcg ttgccgccca tgacgaaacc ctgatggcgg actacggaca ccgcatcctg
    16981 cgcctctcga aaggacgact cgcatgagca tcatccacta cgcctcgctg cacatcgaat
    17041 ccgcccgcac cgcgctcaaa cagcttctgc gccaaccctt cggcacgctg ctcaccctcc
    17101 tgatgctcgc cgtcgcgatg accctgccgc tgtttatgta tttgggcatt caaagcgggc
    17161 aaagcgtgtt gggcaaactc aatgaatcgc cgcaaatcac cgtctatatg gaaaccgccg
    17221 ccgcacaaag cgacagcgat accgtccgca gcctgctggc gcgcgacaaa cggctcgaca
    17281 acatccgctt catcggcaaa gaagacggtt tggaagaatt acagtccaat ctcgaccaaa
    17341 atctgatttc catgcttgac gacaaccccc tgccggatgt ctttatcgtt acccccgacc
    17401 cggcaaccac gcccgcccaa atgcaggcaa tctaccgaga cattaccaaa ctgcctatgg
    17461 tcgaatccgc caacatggat acggaatggg tacagacact gtaccaaatc aacgaattca
    17521 tccgtaaaat cttatggttt ctctccctga cgctgggtat ggcgttcgtc cttgtcgcgc
    17581 acaacaccat ccgcctacaa atcctcagcc gaaaagaaga aatcgaaatc accaaactct
    17641 tgggcgcgcc cgcgtcgttt atccgccgcc cattccttta tcaagccatg tggcagagca
    17701 tcctttccgc cgccgtcagc ttggggcttt gcggttggct gctctccgcc gtgcgcccat
    17761 tggtcgatgc cattttcaaa ccctacggct tgaacatcgg ctggcgtttc ttctacccgg
    17821 gagaaatcgt gctggtgttc ggcttcgtca tcgcgttggg cgtattcggc gcgtggcttg
    17881 ccaccaccca gcacctgctc ggcttcaaag ccaaaaaata aaaaaaaata aaacaccgtc
    17941 aaaaatgccg tccgaacccg ttttcagacg gcatttcaat ttgccagtat aatggcgcat
    18001 ttttccaaca aggaacctac catgctgacc ccggaacaag taaaagccat gattgaaggc
    18061 gtggcaaaat gcgaacatat cgaagtagaa ggcgacggac accatttttt cgccgtcatc
    18121 gtttcatcag aatttgaagg caaggcacgc cttgcacgcc accgcctgat taaagacggg
    18181 cttaaagccc aactggaaag caacgaactg cacgcacttt ccatttcggt agccgccaca
    18241 ccggcagaat ggaatgccaa agcacaataa ccgccacaca aaaatgccgt ctgaaacagc
    18301 acaacgtttc agacggcatt tttttatatc aaaccgttta ctcgccgcgt ttttccaaag
    18361 cggctacggc aggcaactct ttaccttcca agaactcaag gaacgcgccg ccgccggtgg
    18421 agatgtagcc gatttggtcg gtaacgccga atttggcaat cgccgccagc gtgtcgccgc
    18481 cgcccgcaat cgagaatgct ttgctttggg cgatggcttc ggcaagggct ttcgtaccgc
    18541 ctgcgaactg gtcaaactca aacacgccga ccggaccgtt ccaaacgacc gtgcctgcgg
    18601 ctttgagcag ttcggcaagc tcggcggcgg atttcggacc gatgtccaaa atcatatcgt
    18661 cttcggcaac gtcggcaatg tctttcacga ccgcttcggc atcggcggca aaggctttgg
    18721 caacgacgac atcggtcggc agcggcacag aaccgccttt gtccgccatt tttgccataa
    18781 tttttttgga ttcttccact aaatcgtgtt ccgccaaaga tttaccgatg gctttacctt
    18841 cagccaacag gaaggtgttg gcgataccgc cgccgacgat gagttggtca actttatccg
    18901 ccagcgattc gaggatggtc agcttggtgg acactttgct gccggcaacg atggcaacca
    18961 tcgggcgcgc gggctgtttc agggctttgc ccaaagcgtc gagttcgccc gccatcaata
    19021 cgccggcgca ggcaacgggc gcggcttgag cgacggcttc ggtcgaagct tgggcgcggt
    19081 gcgcggtgcc gaacgcgtca ttgacgaaca cgtcgcacaa agaagcgtag gctttaccca
    19141 gttccaaatc gtttttcttc tcgcctttgt tgatacgcac gttttgcagc atcacgacat
    19201 cgcccgcgtt caaagccggt ttgttttcgc gccagtcgtt caatactttc acgtctttgc
    19261 ccaacagacc gcccaaatgc gcggcaacgg gcgcaacgtc gtcttcgggg tggaactcgc
    19321 cttcagtcgg gcgacccaag tggatcatca cgataacgga tgcgccgttg tccatgcagt
    19381 attggatgga cgcgagcgag gcgcggatac gggtgtcgtc gctgattttg ccgtctttga
    19441 acggcacgtt catatcggcg cggatgagga cggttttgcc ctgcacgttt tgttcggtca
    19501 gttttaaaaa tgccataatc agtccttttc aatcagtgtt tgcgatacag aaacaattga
    19561 tgccgtctga aggcttcaga cggcatcgca acccgatcag ccggatacgc gctcgatttt
    19621 cgcgccgacg ctgccgagtt ttttttcaat attttcataa ccgcgatcca agtggtaaat
    19681 ctgttcgacc acggtttcgc cgcgcgccgc caaaccggcg ataacgaggc ttgcggacgc
    19741 acgcaaatcc gtcgccttga cgactgcgcc ggaaagccgt tccacaccct gcacaaatgc
    19801 cgtattgccc tcggttgtaa tgttcgcccc catccggttc aactcgggga cgtgcataaa
    19861 gcggttttca aaaatcgttt ccaccacgcg gcagcttccc tccgccacgg cattcaatgc
    19921 cataaactgc gcctgcatat ccgtggggaa gccggggtgg acgaccgtgc ggatgtccac
    19981 cgccttcgga cgctgccgca tatcgatggc gatccaatcg tcgcccgcct caatcaccgc
    20041 acctgcctca accagtttgt ccaacaccac ttccatcgtt ttcggcgcag cattccgcaa
    20101 aaccaccctg ccgccggtta tcgccaccgc acacaggaac gtccccgctt caatccggtc
    20161 ggggacaacg ctgtgttcgc agccgtgcag ctcgtccaca ccttccacaa tcattgtgga
    20221 cgtaccgatg ccgctgattt tcgcgcccat tttgaccagg cattccgcca aatcgaccac
    20281 ttcaggctcg atggcgcagt tttccaaaac cgtcgtacct tccgccagcg tcgccgccat
    20341 cagcaggttt tccgtgccgc cgacggtaac gacatccatt gccacacgcg tgcctttaag
    20401 cttacccttg gctttgacgt aaccgtgttc gataacaatc tcagcaccca tcgcttctaa
    20461 gcctttcaaa tgctggtcga cagggcgcga accgatggcg cagccgcccg gcaggctgac
    20521 ttgcgcctcg ccgaaacgcg ccagcgtcgg gccaagcacc aaaatcgagg cgcgcatcgt
    20581 ccggaccaat tcgtaagggg cgcaggtgtt gtttaccgta ccaccgttga tttcaaattc
    20641 actgatattg tcggtcagga cgcgcgcgcc catcccctga agcagctttt gtgtggtttt
    20701 cacatctgcc agcataggga cgtttttcag gcgcaacgta cccgatgtca gcaaacccgc
    20761 gcacatcagc ggcaatgccg cgtttttcgc gcccgagacc gttatttccc cgttgagcgg
    20821 gccgtttgcg gagattttca gtttgtccac gtttgttctt tcctagtggg tacttgtaat
    20881 atttcaatac tcgggacaac gcataaagca tcacccgatg aaggttacag aggcggaatt
    20941 ataagggatt ttcgggaaaa atacggaagc cgcaccaaag aatttgacga aatgccgcgc
    21001 tttccgaaca aggattgtcg gaagacaaaa aaagccgagt tttgaaaact cagctttttt
    21061 tgttttatct ggtgggtcgt gagcgattcg aacgctcgac caacggatta aaagtccgct
    21121 gctctaccga ctgagctaac gacccgataa gctcgaaatt ctatacatca agcctgatgc
    21181 tgtcaaccat tttgtcggcg ctcatacggc attttattta tcggactgtt ttttcgtata
    21241 aattaacgag gtcagtatcg atgcacccaa cgcgccgaac acgaccgaca gcgaaacgga
    21301 aatcgggata tgcacccaat gcattaccag cattttcaca ccgataaaac ccaacacgaa
    21361 tgccaagccg tatttcagga agataaagcg ttccgccaca tccgccagca ggaaatacat
    21421 cgcccgcaag cccaaaatag cgaaaatatt ggaagtcagc acgataaacg gatcggtggt
    21481 aacggcaaag acggcgggga tgctgtccac ggcaaacacg acatcgctca attcaatcat
    21541 gaccagcacc aaaaacagcg gcgtggcgat ttttttaccg ttttcgacag taaaaaattt
    21601 ctcgccgtga aattccgtgc cgaccggaac gactttcttg acggcattca gcagcctgct
    21661 gtttgccaaa tccccttcct catcgccttc gggcttcatc atgtgtatac cagtatagag
    21721 caggaacgcg ccgaacagat acagaatcca ctcgaactgc tgaaccagtg ccgcgccgac
    21781 gaaaatcatg acagtgcgca ataccaatgc gcccaatacg ccgtacagca gcacgcggtg
    21841 ctgaaactgc ggtgcgactt tgaagtagcc gaatatcatc aggaacacga aaatattgtc
    21901 gactgccaac gatttttcca aaatgtagcc ggtaaagaat tccaatactt tttcttttgc
    21961 gactgccgcg ccgtagccgg gattgccggc gagtttgaaa tacagccagc ctgcgaacag
    22021 gcaggatacg gcaacccaca agccgctcca tgccaaggct tctttgacgc cgactttatg
    22081 gctgctgttt ttcttcagcg aaaacatatc caaggcaatc atgaccagca ctgccgcaaa
    22141 aaaaacgccg taaaacaacg gcgacccgat gccgggatat tctgtcatgg ttcaatctcc
    22201 tgatttgaaa tgtagttgtg ttaccagctg atataaaaca tcgcttttgc caaaaagaca
    22261 atcagcagca tatgggtaaa gacgacggtg tgtatgtatt tcgaccagcc gacggtcagt
    22321 gtggaacgcg ccattttgac gacggcgatg gcgaagtgcg ccaacacgct gaacgccaac
    22381 aggattttca gcgtcagcat cgtaccgaag gaagtggcaa acggttcgcc caatatagaa
    22441 agatagcggt ttgccgccat cacgatgccg ctggcgaaca gcagtccgac cacaaacggc
    22501 atcaccctga cggcgcggta agacattgcc ttttccactt cgcgccgcgc ctcgcacgac
    22561 acccgtcccg tatgcaggac ggacaaaacc agcacttcaa aaaacacgcc gccgacaaag
    22621 gcgatggcgc aatacaggtg gacgatgtgc gcgacggcat aaatactcat acgatgctcc
    22681 aaccggaaaa ctcggatacg gattgtatca ctatcgtccc cgatgtccgc ataccgcttc
    22741 ccgtaccgcc tcggcgattc tcgcgcccgc tccgcgatgt tgtgcgataa agccgtccac
    22801 gtgcgcctgc atctgcattc cgcccccctc ggacgataag gttttttcaa cggcttcccg
    22861 ccacgcgtcc gccgactcaa cccgaaccgc cgcccccgat gccaaggcgt gccggcaggc
    22921 ttcggaaaaa ttgtaggttg aaaagccgaa tatcgtcgga acgccgcagg aaagcggttc
    22981 gataatgttc tgacagcccg aatcgaccag actgccgccg acaaaagcga catcggcgca
    23041 caggtaatac gcatacagct cgcccatact gtcgcctatc cacacctgcg tatcaggttc
    23101 gaccggcaaa ccgtcgctgc gccgctgaac cttaaacccg aagcgttttg ccgtttcaaa
    23161 taccgtctga aaatgctcgg gatggcgcgg cacgacgacc agcagcgcat cgccgcgata
    23221 ttgttgccac gccgccagca gtttttccgc ctcgtcttca ccccgataaa cgcgcgtgct
    23281 gccgcacacg gcaaccggcc tgcctccgat gcgtttttca aactgccccg ccagcgtttt
    23341 catctgttcc gacggcatca ggtcgtattt ggtattgccg cacacctgca cggatgccgc
    23401 gcccaatttc gccaaccgcg ccgcatccgc ctccgtctgc gccagacaac ccgtcagcga
    23461 agcggcggca ggacggatca ggcggcggac tttcagataa ccgctcaacg atttttccga
    23521 cagccgcgca ttcgccaaaa acagcggcac acccgcgcgc cggcattcct tcatcagatt
    23581 gggccagatt tcggtttcca tcaaaatgcc gaacatcggg cagtgttcgc gcaaaaactg
    23641 ccgtacccac gtttttttgt catacggaag atagcggcat tgcgcatcgg gaaacagaac
    23701 ttgcgcggtt tcccgtcccg tcggggtcat ctgcgtcatc agcagcggtg catcgggaaa
    23761 acgctgccgc aactcgcgta tcaagggctg ggcggcacgc gtttctccga ccgaaacggc
    23821 gtgtatccaa accgcgccgg taacgggatt cggatacggc ttgccgaaac gctcgtcccg
    23881 atgcgcccga tatgcagggg cacttccgga gcgtttgtcc aaataacgcc gtatccatat
    23941 cggcgcaagc agccacaata catcataaag ccattggaac atctttctat ttcctgcaaa
    24001 acaaatgccg tccgaacggt tcggacggca tttcggcaac ggaatcaaat atcgtaggtt
    24061 gtcgaagcgg tatctccgcc cttgcccgtc cagttggtat ggaaaaactc gccgcgcggt
    24121 ttgtcggtgc gttcgtaagt gtgcgcgccg aagtagtcgc gctgtgcctg caagaggttg
    24181 gcaggcagac gttcggtcgt gtagccgtcc aagaacgtaa tcgccgaagc catgcagggc
    24241 atagggatgc cgcattcgac tgccttggca accactttgc gccacgccgg caggcagttt
    24301 tccagaatat ttttgaaata cggatccgta cccaagaaca ccaaatcggg attgttttca
    24361 tacgcgtcgc ggatattgct taagaatgcg ctgcgaatga tgcacccctc gcgccacagc
    24421 agcgcagtgt tgccgtagtc caaatcccag ccgtagcttt cgcccgcttc gcggatcagc
    24481 ataaagcctt gtgcgtagga aatgattttg gatgcaagca gggcctgtct caacgcctca
    24541 acccattctt gtttgccgcc ttcgacgggc gtaacggttc gggcgaacag cttgccggtc
    24601 tgcacgcgct gttctttgaa cgacgaaacg cagcgggcga ataccgcttc ggaaatcagc
    24661 gtcaacggaa tacccaaatc caaagcattg atacccgtcc atttgcccgt gcctttttgc
    24721 cctgccgtat cgaggatttt ctcgaccagc ggttcgccgc cttcgtcttt atagcccaaa
    24781 attgccgctg tgatttcaat cagataagaa tccagctcgg ttttgttcca cgcggtaaac
    24841 acgcggtgca tttcgtcgta ggacagtccc aaaccgtctt tcataaactg gtacgcttcg
    24901 caaatcaact gcatatcgcc gtattcgata ccgttatgca ccattttgac aaaatgtccc
    24961 gcgccgtccc tgccgaccca gtcacaacac ggttcgccct gcggcgtttt ggcggcaatc
    25021 gcctgaaaaa tcggtttgac ggcttcccaa gcgcgcttat ccccgcccgg cataatggac
    25081 gggccgtgcc gcgcgccttc ttcgccgccg gatacgcccg ccccgacaaa caaaatccct
    25141 ttttcggcaa ggtaatgtgt ccgccgtgtc gtgtcggggt aattggcatt gccgccgtcg
    25201 ataatgatgt cttcctcgtc caacaggggc agcagttgtt cgataaagtc gtcgacgacc
    25261 gaacctgcgc gaaccatcat catgattttt ctcggttttg ccagcttatc gaccaagtct
    25321 tgcagggaat acgcgccgac aatatccgtt cccttagccg cgccgtttaa aaattcgtcc
    25381 accttaccga ttgttcggtt gtaggcgaca accttaaatc cgcaatcgtt catattcaaa
    25441 atcaggtttt gccccataac cgccaaaccg attacgccaa tatcgccttt cattgcagga
    25501 agctccgtta tagatttaat ttatcgaccg caactctacc tgatttacac ttgtttaaca
    25561 atccttaact ctttaatttt ttgaaaagat gcctttacgc ttttccgtgc gtttccctga
    25621 aggcttataa ataaaatata aaatttaaat aataaaacga tgattatatt gataggagga
    25681 attttctgtg ggtaactttt ttttatttta aaaatcattg ggatttccgt tttaaaaggg
    25741 tgtcggtaag gcggattccc ttttgtgcat acctgtggat tgtttttcat gaagaatagt
    25801 ttttgtggac agtttgcttg ttgtgcagat ggcatcctac ttttctttac cgaatggctg
    25861 ccggtgcctt taagaacggg aatactgtgg agatttaagc cggaagtgtg tttggaacgt
    25921 gtggaaatgg tcaggtgtcg gcacgaatgt cttatttctg catatcggca gagtgcgcat
    25981 ccgaatttgt gtataagtgg tggaaaaaat gagatttgcg ggtaaatctc acaatatttc
    26041 agtctgataa ctttggattg cttgtgtata aataaacttt cggatgggga tacgtaacgg
    26101 aaacctgtac cgcgtcattc ccacgaacct acatcccgtc attcccatga aagtgggaat
    26161 gatgaaattt tgagttttag gaatttatcg gaaaaaacag aaaccgctcc gccgtcattc
    26221 ccgcgcaggc gggaatccag cctcgtcggt acggaaactt atcgggaaag cggtttcttt
    26281 agattttgcg ttctggattc ccacttttgt gggaatgacg ggattaaagt ttcaaaattt
    26341 attctaaata actgaaactc aacgaactag attcccgctt ttgcgggaat gacgaatttt
    26401 aggtttctgt ttttggtttt ctgtttttga gggaatgatg aaattttaag tttgaggaat
    26461 tta
//
LOCUS       DAWFLT010000021        25926 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWFLT010000021 DAWFLT010000000
VERSION     DAWFLT010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25926
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.021"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(207..494)
                     /locus_tag="WGR74_000904"
     CDS             complement(207..494)
                     /locus_tag="WGR74_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905352.1"
                     /translation="MDDLIAAFLGCLSGKYSEGKISRLEISLTAGSIFFAVFFIHELI
                     IFIVDGNKENLWSFITGIMKDLFLFSCSVCLLFYFLLFIAETIGKTINKIK"
     gene            complement(801..1172)
                     /locus_tag="WGR74_000905"
     CDS             complement(801..1172)
                     /locus_tag="WGR74_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905353.1"
                     /translation="MRFYIDNKECDYIFFRKAVNISDDFIQSGFITPEGLITKNSNPK
                     LFNQYSKMVSKNKCQYEMVTFLSDEMKNIIELLSNDDAYIEFAYSEDFYVLPEIEIDK
                     RTLKSLNFYIDFGVKYIINKI"
     gene            complement(1306..1749)
                     /locus_tag="WGR74_000906"
     CDS             complement(1306..1749)
                     /locus_tag="WGR74_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905354.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGAAAPLVAIGILHAGRFLAQRWVTQRVAAQALSRGANVYARTSQQAR
                     AVANQAWGRQNVIRHGQKEIHSNYSHFQNSSQKIRGHAFYGNKHR"
     gene            complement(1792..2661)
                     /locus_tag="WGR74_000907"
     CDS             complement(1792..2661)
                     /locus_tag="WGR74_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ0905355.1"
                     /translation="MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGTQPDRIDGKKETARNTSTYAHFGTGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            2825..3748
                     /locus_tag="WGR74_000908"
     CDS             2825..3748
                     /locus_tag="WGR74_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0905356.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGATLDEHTLFEPGKVMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(3826..4467)
                     /locus_tag="WGR74_000909"
     CDS             complement(3826..4467)
                     /locus_tag="WGR74_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0905357.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(4522..5313)
                     /locus_tag="WGR74_000910"
     CDS             complement(4522..5313)
                     /locus_tag="WGR74_000910"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ0905358.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            5479..5554
                     /locus_tag="WGR74_000911"
     tRNA            5479..5554
                     /locus_tag="WGR74_000911"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5512..5514,aa:Thr,seq:tgt)
     gene            5589..5664
                     /locus_tag="WGR74_000912"
     tRNA            5589..5664
                     /locus_tag="WGR74_000912"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5622..5624,aa:Thr,seq:tgt)
     gene            5806..6597
                     /gene="panB"
                     /locus_tag="WGR74_000913"
     CDS             5806..6597
                     /gene="panB"
                     /locus_tag="WGR74_000913"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ0905359.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTESVSCPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTVKFVKNFMQGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            6587..6763
                     /locus_tag="WGR74_000914"
     CDS             6587..6763
                     /locus_tag="WGR74_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905360.1"
                     /translation="MQTEQGLLPMPSESRFRRHFVLPCAARIIGAFCRAGSPKDKDYR
                     YANHTYHSRTARVA"
     gene            6720..7556
                     /locus_tag="WGR74_000915"
     CDS             6720..7556
                     /locus_tag="WGR74_000915"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ0905361.1"
                     /translation="MQIIHTIQELRAWRKNAGTVAFVPTMGNLHEGHLALVREAKKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFLGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEIRRVDTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            7721..9559
                     /locus_tag="WGR74_000916"
     CDS             7721..9559
                     /locus_tag="WGR74_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905362.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVGKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGEAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMFALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            9699..10280
                     /locus_tag="WGR74_000917"
     CDS             9699..10280
                     /locus_tag="WGR74_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ0905363.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            10290..11135
                     /gene="ispE"
                     /locus_tag="WGR74_000918"
     CDS             10290..11135
                     /gene="ispE"
                     /locus_tag="WGR74_000918"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ0905364.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKSRDDGKIILHNPVDGMPQEADLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSDLYEAYLAEGLSKH
                     PLLSV"
     gene            11143..11218
                     /locus_tag="WGR74_000919"
     tRNA            11143..11218
                     /locus_tag="WGR74_000919"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11176..11178,aa:Gln,seq:ttg)
     gene            11231..11306
                     /locus_tag="WGR74_000920"
     tRNA            11231..11306
                     /locus_tag="WGR74_000920"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11264..11266,aa:Gln,seq:ttg)
     gene            11315..11390
                     /locus_tag="WGR74_000921"
     tRNA            11315..11390
                     /locus_tag="WGR74_000921"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11348..11350,aa:Gln,seq:ttg)
     gene            11557..12540
                     /locus_tag="WGR74_000922"
     CDS             11557..12540
                     /locus_tag="WGR74_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ0905365.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            12607..13179
                     /locus_tag="WGR74_000923"
     CDS             12607..13179
                     /locus_tag="WGR74_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ0905366.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTTVEVLALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            13510..15474
                     /locus_tag="WGR74_000924"
     CDS             13510..15474
                     /locus_tag="WGR74_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0905367.1"
                     /translation="MIDGRRNYGNRTEPNRTEIIHDLENNFKQNNILKLKQLFPEVFC
                     EDQIDFEKLKLVLGAENLAGAGERYQLDWAGKTAAYLNLQSPTSRTLVPCKEESANFD
                     GTQNVFIEAENLEALKILQKAYAGSVKMIYIDPPYNTGSDSFIYPDKFSESRDEYARR
                     VGDTDDAGYLKRDGVFQGAWRKNGKDSGHYHSNWLSMMLPRLHLAKTLLREDGVIFIS
                     IDDNEQAQLKLLCDEVFGAENFVNQIAVKMSELSGVKMKHLNQYAKLKEFLLIYAKNI
                     HFANFNIEKKRKSPETLSKYLKYYSSIIENIESECEQWKIISLDEYFKDKNIILDREK
                     LNDWKLSNAQRLVYRTNSKTVDKFLLKNPNAPDICKLINDDGKEIIKWGNKEMLFLEK
                     YIDEYLGDIWLDISTINLNKETHTLVFENGQKPLTLIKRLLKSLETENNIVMDFFSGS
                     GTTAHAVMQLNAEDGGNRRFICVQLPEETDEKSEARKAGFDTIAEIAKERIRRAGRQI
                     SDGLQDGSETDTGFKVFKLAESGFKQWRQPEQSDTEALQRELSLNIDSVLSETSSENL
                     LYELMLRMGLKLTCKVSFLDDVYFVEDEDTGGLYAFLLKRVDQGLIDAVLAKHPAKVA
                     ALDRLFEGDDALKSNTVLQMKDAGVMFECV"
     gene            15709..18360
                     /locus_tag="WGR74_000925"
     CDS             15709..18360
                     /locus_tag="WGR74_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ0905368.1"
                     /translation="MELKFEELPYQLDAVNAVANLFAGQPNHARTFDLTSQGTGRFVG
                     NGLDLDWETLGLNLNTVQKQNGQPETEIGAHGLNFSLEMETGTGKTYVYLRTIYELNR
                     SYGWKKFVIVVPSVPIREGVLKSLEITKAHFDGVFGRPVMRYAEYSSSRLSDLRTFAV
                     NDHIEILVINIQSFEKDGNVINKVNESGETPIKFIQETCPIVIIDEPQNMETEGRLNA
                     LESLNPLFTLRYSATHKQPYHKVYSLNPVQAYNLKLVKQIEVLPVLAENDVNGAFVEL
                     LEVKQAAKGRPKADLNIHYQDKKETKKKKVSVKWEDDLFEKSGGNEAYRHGFIIDGMD
                     FDKQELAFSSGKTITRGGDGDAVRDEIMKNQIRETVREHLAKEKRLNPLGIKVLSLFF
                     IDRVENYRTEDGTAGKFALWFEEIYRELAGNTDTAGVHDGYFSQDRKGRLKNTDGTTQ
                     ADNDTYRLIMRDKETLLSFDSPLRFIFSHSALKEGWDNPNVFQICTLNETRSPLKKRQ
                     EIGRGLRIAVNQEGRRVRDENVNILTVVPNESYELFAANLQKEYEDECGIKFAAENIK
                     NGNKKKKQLFRKDFPLDPEFQAIWDKLKYKTRYRVRFDTEELIKQASEAVANLPEIQK
                     PKIRIRKAQLKQSQTYGIETVEIAGRSREMDVQWEIPDILGEIQRKTGLTRRTVYEII
                     IQSGRLKDVAGNPQRFIDLTAEAVNRSLHALMAAGIEYERTGEVYDQKLLEDMAREGI
                     EFYENGFTFTVSNPQKTICENYIPLDSGTEKAFAQNCEDYDAKNEGGVAFYFKLPGWF
                     KIPTPLGNYNPDWAVVKQVRDKVYFVAETKNTGKGIQTGVDMDKLRQNERLKIKCGKR
                     HFEALGGLAYRVVEKVGDLKSEKEEQA"
     gene            complement(18522..19691)
                     /locus_tag="WGR74_000926"
     CDS             complement(18522..19691)
                     /locus_tag="WGR74_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ0905369.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQVLGKIKIRQNGYTIAEKEIVALENVEKRSRWQRLWTRLTGQ"
     gene            19839..21365
                     /gene="ilvA"
                     /locus_tag="WGR74_000927"
     CDS             19839..21365
                     /gene="ilvA"
                     /locus_tag="WGR74_000927"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ0905370.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(21422..22495)
                     /locus_tag="WGR74_000928"
     CDS             complement(21422..22495)
                     /locus_tag="WGR74_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ0905371.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGKILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(22492..23352)
                     /gene="cysW"
                     /locus_tag="WGR74_000929"
     CDS             complement(22492..23352)
                     /gene="cysW"
                     /locus_tag="WGR74_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ0905372.1"
                     /translation="MKPYSANPNLTEPRWLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            complement(23607..24443)
                     /gene="cysT"
                     /locus_tag="WGR74_000930"
     CDS             complement(23607..24443)
                     /gene="cysT"
                     /locus_tag="WGR74_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ0905373.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            24582..24689
                     /locus_tag="WGR74_000931"
                     /pseudo
     CDS             24582..24689
                     /locus_tag="WGR74_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            24734..25066
                     /locus_tag="WGR74_000932"
     CDS             24734..25066
                     /locus_tag="WGR74_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ0905374.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            25398..25907
                     /locus_tag="WGR74_000933"
     CDS             25398..25907
                     /locus_tag="WGR74_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ0905375.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT         6626 a         5887 c         6968 g         6445 t
ORIGIN      
        1 aatttactcc actatacaaa acaatcaaga aactattggc ggtatgtata atcttgaatt
       61 tctttttttg atgaaattaa aataatgaaa tatatggtaa acgttacaac ggaagcctta
      121 gcctcccgat tgcagtattt caattccaag ataaatgccg catagaagat aaaatactca
      181 aatggttttt tagcggctta aatatcttat ttaatcttat tgattgtttt cccaatggtt
      241 tccgcaataa ataataagaa gtagaataat aaacaaacag aacatgaaaa taaaaataaa
      301 tctttcatta tcccagtaat aaaactccaa agattttcct tatttccatc aacaataaaa
      361 attatcagtt catgaataaa gaatactgca aagaaaatgc ttcctgccgt tagagatatt
      421 tctaatctgc ttattttccc ttcgctatat ttacctgata aacagcctaa aaaagctgca
      481 atcaaatcat ccattttaaa aatttctgta aaaacaggta atgtctttaa ttcattacct
      541 gttttgccat tatttatagt ggattaaatt taaatcagga caaggcgacg aagccgcaga
      601 cagtacaaat agtacggcaa ggcgaggcaa cgccgtacta atccactata tatatccatt
      661 catatagaga aaaattctta tacccaatat aaaacaaggt ctttcattat attttgaaaa
      721 acctagaagc tttgtatcta ttggtttatc aaaattaata attattgcag aagacatgta
      781 ggctaataat cttgatttat ctatatttta ttaattatat atttaacccc gaagtcaata
      841 tagaaattta gtgattttaa agttctctta tcaatttcaa tctcagggag tacataaaaa
      901 tcctcagaat aggcaaactc tatatatgca tcatcattcg ataataattc aataatattt
      961 ttcatttcat ccgaaagaaa tgtaaccatc tcatattgac atttattttt agataccatt
     1021 tttgaatatt gattaaataa ttttggatta ctatttttag taattaatcc ttccggagta
     1081 ataaaaccac tttgtataaa gtcatcactt atattaacag ccttccgaaa aaaaatataa
     1141 tcacactctt tgttatcaat ataaaatctc atactcttta ataatgaaat ataagaattt
     1201 ccagaattac aagcaatttg aaaaaacatc attactccta aaactcataa atagtaagga
     1261 atatatatat atgcatattc cttactattt tttcacaaat tatgcttatc tatgcttatt
     1321 tccataaaaa gcatgccctc gaattttttg acttgagttc tggaaatgag aataatttga
     1381 atggatttcc ttctgtccat gtctaataac attttgtctt ccccaagctt ggtttgctac
     1441 cgctcttgct tgttgagatg ttcgtgcata gacattagct cctcttgata aagcttgtgc
     1501 tgcaacacgc tgcgtaaccc aacgctgcgc aaggaatctg cccgcatgca aaattcctat
     1561 tgccaccaac ggtgccgcag ccccttcagt ttctttcatc tccttttgag aaagctccgc
     1621 caactgcatc ggcgcatctg cctgcgtgtg gaacacttgg ttttcaaatg tctgattgtc
     1681 caagccgttt gccattgcgg gggcaatcat agacagcatc attacggctg cggtgatttg
     1741 ttttttcata ataactcctt tggattacaa ggttggaaaa tcaaaacctg cttaaaacgt
     1801 atgctgtacg ccaaatttca gttcggaact gctttgccct gaaacgttga aacgtgcgga
     1861 tgcgtttaaa gccgtggttt tggtgaaacc gaaacctgtg ccgaaatgag cgtaggtgga
     1921 tgtgtttctt gcggtttctt ttttgccgtc tatgcggtcg ggctgtgtgc ccagccattg
     1981 gatgcctccg gtcaggctga ttctgtcgtt ggcggcaaat gagatgttgg ggttgagcag
     2041 caggtagttg cccgatttgt agcggatgcc gtctgaaagg gttttgctgc cgttgatgcg
     2101 gtaggcggcg gtgagggaaa ggacaatcgg atctatggct ttgtaggtgg tggcgccgat
     2161 gagccaggat tttcccgacg aggctttgtt gcgcgatttt tcgtaaaccg tgctttcaag
     2221 aaagctgatt agggcggggt ttttgtcgtc tttaaggaaa gtgtggctga tgccgaggga
     2281 tacgtcggac atccgtttgt tgcgggtttt gccgttgccg tcgagtttgc gttcttcgtg
     2341 ccacagatag ctgccgctgc cgtaaatgtc ggtattcccg gtcagtccgt agcgcaaacc
     2401 gagcgtgccg acgagcatat cggtattgct gccgttttct tggatttcgg tcggaatggg
     2461 gataaacgag gttgcgccgg tttgaatgta aaccggtgcg gcaagttcgg cgcggttgtt
     2521 ttcgctgttc aggtaggtaa gggaagtttc cagtttccat tttcccttgt cggtcattat
     2581 gtcttcaatc gtcaagggca ggtcggcata agtggataaa ggcaggatgg cgggcaaggc
     2641 gggcaaaaag atgcgcttca tatttctcct attgatatta attcttattt actgataata
     2701 atgaatatcc gataatcttg tctaaataaa tttttaaatc ataaaaaaca atatgtttgt
     2761 ttttgtcaat tatgtttcga tatgccgtct gaaaagtttg tgaaaaacgg ttacaatccg
     2821 ccgcatgaaa aaacgcaata atcctcttcc tctgttgaac ggtgtcaaac ccagttattt
     2881 ggtgctgccg catgaaaagc agttttacgg gctgccgctg ctgcattttc tgtgcatccg
     2941 ctttcctttt gtgggcgcgg acgattggcg caggcggttg aacagcggtt ttgtggtcgg
     3001 ttcggatggt gcgacgttgg acgaacatac tttgttcgag ccgggtaagg tgatgtttta
     3061 ttatcgggaa accagcagtg aggatgagcc gcgtattccg tttgaagaaa agattttgca
     3121 tattgatgag catttgattg tggtggacaa gccgcatttt ctgcccgtca tccccagcgg
     3181 caggtttttg cgggaaaccc tgctcacgcg cctgcgtttg cggcctgaat tgcagcattt
     3241 gaatgttgag gacattacgc cgctgcaccg cttggacaag gatacggcag gcgtgatgct
     3301 gctgtcgcat aatcctgcca cgcgcggagc ctatcagacg atgtttcaaa acaaaacggt
     3361 atggaaaacg tatgaggcgc ttgcgccgac aaggacggat ttgccgtatc cgctcgatgt
     3421 ggtttcgcgt ttggtgaggg gtgagaaatt ttttacgacg caagaggcgg aaggcgaacc
     3481 aaacgcacat acgacggtcg aactgattga aaacaggggg gaattcagcc tttaccgcct
     3541 tacgccgcat acgggcaaga aacaccagtt gcgcgtgcat atgatgggtt tgggtatgcc
     3601 gctgttgaat gacgcgctct atcccgtgcc gtctgaagcg ggcagcgagg attatcggaa
     3661 acctttgaag ctattggcaa aaaagattgc gtttgcagat cctttgtcgg gtcgggaaag
     3721 ggtgttttgc agcggatttt gcctataatg agagggaacg caaacccgta tcggcggatg
     3781 ccgccgatac gggttttaca ttatcgatgc cgtctgaaag gggttttatc cgttcaggca
     3841 tacgggcgcg aagcggagga tggtgtcgcg caggacgatg agtttgccgt gaaagaaatg
     3901 cgtggaaccg gcgatggtaa tgacgggcaa atcttgcggt tccgcccatt tcagtgcttt
     3961 cccgatttcg acgacttcgt cttccgcgcc gtgtatcatc agcgttttgg caacattggg
     4021 aacggcggac ggctcggggc ggccggtata gtggcagact gccgcgccga tgagcagcaa
     4081 taaatcgggt atgcgcgctt gtgcggcaaa tgtggcgaca taaccgccga aggagaagcc
     4141 ggataaggca aattcggggg cttcgggatg ttgggcacgg gcatagtcga tgacggcgag
     4201 gcagtcttgc gtttcgccgc gtccgtaatc gtgtgtgcct tcgctgccgc ctacgccgcg
     4261 aaggttgggt aggtagcagt ggaagccgag tttgcttaag gctttggcgg cagtttggat
     4321 aactttgttg gtgtttgttc cgccctggag ggggttggga tgattgatga cggcaacacc
     4381 gcgtgccggt acttgttcgg acgggatatg gatggtttct aaaatgccgg caggaccgga
     4441 tatgtgtatg gtttcgggtt tcagcataat gatttcctct tgattttccg catgttccaa
     4501 tgcagccgtg aagatagttg cttaaaggtg gaaatgcttg ccgttgttgg ggttggacgc
     4561 tttcaggccg gcaagcatac ggtggaagtc caatccgtat gcgttgctca gtttgtcggc
     4621 acgttttttg agtttgtcta tgttttgctc tttggggctg ctttggaagg ccattaccgc
     4681 gacattgccg tggctttcgg caggaagttc caagacgcgc ccttcaaaaa cgctcaacaa
     4741 ccgttcgatg aagcgttggt agcgtttgtc gccgctccac cagttggtta cgaatatgcc
     4801 gtctgaagag agtgcgtttc ggcagtctcg gaaaaacggt tcttcaacca gcgcatcgat
     4861 gatttgttcg ccgtcgaacc cgtccaccaa aatcacatcg gtgttgtggc ggaagacttt
     4921 gatatattct gcaccgtctg cttcaataat ttcaaatttc tcgccctcga aaggcaactc
     4981 aaacaggttg cgggcaatgg cgatgacctg aggattgatg tccacggcgg tttggcgcgt
     5041 gtcgggcagg taggtgtcta tccagcgtgc aaacgagccg ccgcccaagc cgatttgggt
     5101 gatatgctgc ggaagtgcgt cggtaaacag cagccagccc atcatcgcgc ggctgtaaga
     5161 gagtaccagc tcggacgggt ggtcgaggtt cattgagctt tgaacggtgt cgctgcccaa
     5221 gtgaagcgaa cggatattgc cttcttcgga aatgccgact tcgggaaagc cggattttgc
     5281 aggacgcagg cggcggtagg gatgtcttgc catcggtgtg aacggtcggt ttgaaaggcg
     5341 gatattttat cagaatgccg atgcttgtcc tgcttcaaat taatttcttt tcaaataaat
     5401 tacttattcg gatttgccgg ggctttcgga taaattcctt gccaaagtgt gaagttgcct
     5461 gcataattcg ccttctttgc cggtatagct cagttggtag agcacctgac ttgtaatcag
     5521 ggggtcccga gttcgactct tggtgccggc accatttctg ctgatggtgt tgcaaaacat
     5581 ttcagtaagc cggtatagct cagttggtag agcacctgac ttgtaatcag ggggtcccga
     5641 gttcgactct tggtgccggc accagatttg acagtccgat tgtgtaaaac agtcgggctg
     5701 ttttgcattt gcggcacggt cggtggcagg gctgcgggca tttcactata atagcccgtt
     5761 tcaaactgaa aaggccgtct gaaaagggcg gggtaacaat atctgatgat tactgtgaac
     5821 acactgcaaa aaatgaaggc ggcgggcgag aaaatcgcta tgctgaccgc ttacgaatcc
     5881 agttttgccg cgctgatgga cgatgccggc gtggatgtgc tgctggttgg ggattctttg
     5941 ggaatggcgg ttcaagggcg gcagtcgacg ctgcccgtca gcctgcgcga tatgtgttat
     6001 cacaccgaat gtgtagcacg cggtgcaaaa aatgcgatga ttgtcagcga tttgccgttt
     6061 ggtgcatatc agcagagtaa ggagcaggcg tttgccgccg ccgccgaact gatggctgcc
     6121 ggcgcgcata tggtcaaact cgaaggcggt gtgtggatgg cggaaacgac cgaatttctg
     6181 caaatgcgcg gtattccggt ctgtgcccac atcggtctga ccccgcaatc cgtgtttgcc
     6241 ttcggcggat acaaggttca ggggcgcggc ggcaaggcgc aggcgttgct taacgatgcc
     6301 aaggcgcatg atgatgcggg ggcggcggtc gtgctgatgg agtgtgttcc tgcggaactg
     6361 gcaaaaaagg taactgaatc cgtttcctgt ccgaccatcg gcatcggcgc gggtgtcgat
     6421 tgcgacgggc aggttttggt gatgcacgat atgctcggca ttttcccggg taagacggtg
     6481 aaattcgtca aaaactttat gcaggggcaa agcagcattc aggcggcggt tcgggcgtat
     6541 gttgccgaag tcaaagccaa aaccttccct gctgcggaac atatttttgc agactgaaca
     6601 aggtttgctg ccgatgccgt ctgaaagccg tttcagacgg cattttgttt tgccgtgcgc
     6661 ggcgcgtata atcggcgcgt tttgtcgggc aggaagcccg aaggataagg attaccgtta
     6721 tgcaaatcat acataccatt caagaactgc gcgcgtggcg taaaaatgcg ggaacggtgg
     6781 catttgtgcc gaccatgggc aatctgcacg aagggcatct tgccctcgtt cgcgaagcca
     6841 aaaaacgcgc cgacaacgtt gttgtcagca tattcgtcaa ccgcctgcaa ttcggtcagg
     6901 gcgaggattt cgacaaatat ccgcgtactt tgcaacagga tgcggacaag ctggaagccg
     6961 agggtgttgc cgttgttttc gcgcccgatg agaaagaact ctatccgaac gtggaacagc
     7021 gttacaacgt cgaaccgccc aatctgcaaa atgagttgtg cggcaagttt cgtccgggac
     7081 atttcctcgg ggtggcgacg gtggtgtcca agctgttcaa tattgtcgcg ccggatgttg
     7141 cctgtttcgg caaaaaagac tatcagcagc ttgccgtgat taaaggtttg accgaggatt
     7201 tgaattttga tgttgaaata gtgcctgtcg atacggggcg cgcggaagac ggtttggcac
     7261 tctccagccg caaccagtat ttgagtgcgg cggaacgcga cgaagcaccg cgcctgtacc
     7321 gcgaattaaa ggctgttgcc gaatccttgg cgcagggcag tttggattat gcaggtttgg
     7381 aaaaacgtgc cgtccaatcc ctgacagaat acggctgggt ggtcgattat gtcgaaatcc
     7441 gccgcgtcga tacgctcgaa gtggcgcggg cgggagataa gaaactggtg gtcttggccg
     7501 ccgcccgtct ggggacgacg cgcctgattg acaatttgga aataaaactc ccttaaaccg
     7561 caagcgtcgg gaatgccgtc tgaagcggat ttgcgtttca gacggcattt atttttttga
     7621 acggggtttc gcaaatctac aaacgattct gcttgtgata aagttacgcc tgattatatg
     7681 ccgtctgaaa gttcggacgg ctgtcggata aaggaagatt atgttacccg cccgtttcac
     7741 cattttatct gtgctcgcgg cagccctgct tgccgggcag gcgtatgccg ccggcgcggc
     7801 ggatgcgaag ccgccgaagg aagtcggaaa ggttttcaga aagcagcagc gttacagcga
     7861 ggaagaaatc aaaaacgaac gcgcacggct tgcggcagtg ggcgagcggg ttaatcagat
     7921 atttacgttg ctgggagggg aaaccgcctt gcaaaagggg caggcgggaa cggctctggc
     7981 aacctatatg ctgatgttgg aacgcacaaa atcccccgaa gtcgccgaac gcgccttgga
     8041 aatggccgtg tcgctgaacg cgtttgaaca ggcggaaatg atttatcaga aatggcggca
     8101 gattgagcct ataccgggtg aggcgcaaaa acgggcgggg tggctgcgga acgtgctgag
     8161 ggaaagagga aatcagcatc tggacggact ggaagaagtg ctggctcagg cggacgaagg
     8221 acagaaccgc agggtgtttt tattgttggc acaagccgcc gtgcaacagg acgggttggc
     8281 gcaaaaagca tcgaaagcgg ttcgccgcgc ggcgttgaaa tatgaacatc tgcccgaagc
     8341 ggcggttgcc gatgtggtgt tcagcgtaca gggacgcgaa aaggaaaagg caatcggagc
     8401 tttgcagcgt ttggcgaagc tcgatacgga aatattgccc cccactttaa tgacgttgcg
     8461 tctgactgca cgcaaatatc ccgaaatact cgacggcttt ttcgagcaga cagacaccca
     8521 aaacctttcg gccgtctggc aggaaatgga aattatgaat ctggtttccc tgcacaggtt
     8581 ggatgatgcc tatgcgcgtt tgaacgtgct gttggaacgc aatccgaatg cagacctgta
     8641 tattcaggca gcgatattgg cggcaaaccg aaaagaaggt gcttccgtta tcgacggcta
     8701 cgccgaaaag gcatacggca gggggacggg ggaacagcgg ggcagggcgg caatgacggc
     8761 ggcgatgata tatgccgaca gaagggatta caccaaagtc aggcagtggc tgaaaaaagt
     8821 gtccgcgccg gaatacctgt tcgacaaagg tgtgctggcg gctgcggcgg ctgtcgagtt
     8881 ggacggcggc agggcggctt tgcggcagat cggcagggtg cggaaacttc ccgaacagca
     8941 ggggcggtat tttacggcag acaatttgtc caaaatacag atgttcgccc tgtcgaagct
     9001 gcccgataaa cgggaggctt tgagggggtt ggacaagatt atcgaaaaac cgcctgccgg
     9061 cagtaataca gagttacagg cagaggcatt ggtacagcgg tcagttgttt acgatcggct
     9121 tggcaagcgg aaaaaaatga tttcagatct tgaaagggcg ttcaggcttg cacccgataa
     9181 cgctcagatt atgaataatc tgggctacag cctgctttcc gattccaaac gtttggacga
     9241 aggcttcgcc ctgcttcaga cggcatacca aatcaacccg gacgataccg ctgtcaacga
     9301 cagcataggc tgggcgtatt acctgaaagg cgacgcggaa agcgcgctgc cgtatctgcg
     9361 gtattcgttt gaaaacgacc ccgagcccga agttgccgcc catttgggcg aagtgttgtg
     9421 ggcattgggc gaacgcgatc aggcggttga cgtatggacg caggcggcac accttacggg
     9481 agacaagaaa atatggcggg aaacgctcaa acgtcacggc atcgcattgc cccaaccttc
     9541 ccgcaaaccc cggaaataat gcaggtccat cctttcagac ggcataaggt ttgccgggaa
     9601 gctgcggcat tcgggcaaac ggcacgcagt tcgcacgcgt tttgcacggc acgccgaacc
     9661 cgccggccgg caggatggca cccgttaagg aaattctgat gaaacacacc gtatccgcat
     9721 cggtcatcct gcttttgacc gcttgcgcgc aattacctca aaataacgaa aacctgtggc
     9781 agccgtccga acacatcagc agttttgcag cagaagggcg gttggcagtg aaagcggaag
     9841 ggaaaggttc gtatgcaaat ttcgattgga cataccaacc gcccgtggaa accatcaata
     9901 tcaatacccc tttgggcagt acgctcgggc agttgtgtca agacagggac ggcgcattgg
     9961 cagtggacgg caaaggaaat gtctatcagg cggaaagtgc ggaagaattg agcaggcagc
    10021 tggtcggttt caaactgcca atccaatatc tgcatatctg ggcagatggc aggcgtgtgg
    10081 cgggcgcgcc ttaccgcatc ctgccggacg gcatattgga acaatacggt tggactgtcg
    10141 gcagaaccgc cgacagtggg gggcaagtcc gaacgttgca actgaataac ggaaatttga
    10201 acatcaggct ggttttcacc gaaatcggta tgccgtctga aactgaaacc ccggaacgct
    10261 gtgcggcgcg cacgagataa ggcggacaga tgaatattgc ggacggacgg caggcgtttc
    10321 ccgcacctgc aaaactgaat ctcgatttga ggattaccgg caggcgggaa gacggttatc
    10381 acaatatcga aagcatattc tgcctgatag atttgcagga taccgtatat ttgaaatcga
    10441 gggatgacgg caaaatcatc ctgcacaatc cggttgatgg aatgccgcag gaagcggatt
    10501 taagctaccg tgccgcatcg ttgctgcaaa aatatgcgcg caaccccgcc ggcgtggaaa
    10561 tatggctgga caaaaaaatc ccgacagggg cgggtttggg cggcggaagc tcggatgcgg
    10621 caaccgtttt gctggtgttg aaccgttggt ggcagtgcgg tctgacgcag cggcagctca
    10681 ttgattcggg cgcggctcta ggggcggacg taccattttt tattttcgga aaaaatgctt
    10741 ttgcaagcgg gataggtgaa aaactgatag gaatggatat tccgaaacag tggtatgtca
    10801 tcgtcaaacc gcccgtccac gtttccactg caaaaatttt cacacacgaa ggcttgacac
    10861 gaaattccgc ctcaagcata atgccgactt tccaaaacct gcaaccgttt agaaatgaca
    10921 tgcaggcagt tgtatttaaa gaataccctg aagtttggaa agcctattcc gaattgtccc
    10981 gatatggatt tgccttaatg acaggttctg gtgcgtgtgt attcacggcg tgtcaagata
    11041 ggaatagcgc atacaatata taccgacaag tttcagattt gtacgaggca tatttggcag
    11101 agggtctttc aaaacatcct ttgttgtccg tataaacatt gttggggagt cgtcaagcgg
    11161 ttaagacact ggattttgat tccagcatgc gaaggttcga atccttcctc cccagccaag
    11221 tcaaacaagt tggggagtcg tcaagcggtt aagacactgg attttgattc cagcatgcga
    11281 aggttcgaat ccttcctccc cagccatatt tttttgggga gtcgtcaagc ggttaagaca
    11341 ctggattttg attccagcat gcgaaggttc gaatccttcc tccccagcca gataaaagcg
    11401 tgtaagcctg cttacacgct tttatttcat agaaataaaa atattgaaat gcctttgttt
    11461 gtgtcggatg ttgcaggtat aatgtcgggc ttggtacaag cagagggaag cattgtgttt
    11521 tctgagcgga agttaaacat aaaatcaggt gagaatatgg ctgcgtacga cagtttgatg
    11581 gtatttacag gcaatgccaa tcccgaattg gcacaacgtg ttgtcaggca tttggacatt
    11641 tctttgggca atgcttccgt atccaagttt tcagacggcg aagttgccgt cgaactgttg
    11701 gaaaacgtac gcgggcgcga tgttttcatc cttcagccga cctgtgcgcc gaccaatgac
    11761 aacctgatgg aaatcctgac gatggcggat gcactgaagc gtgcttcggc aggtcgtatt
    11821 accacagcca ttccgtattt cggctatgcg cgccaagacc gccgtccgcg ttccgtccgc
    11881 gttccgattt ctgccaaact ggtggcaaat atgctgtatt cggcagggat cgaccgtgtt
    11941 ttgactgtcg atttgcatgc cgaccagatt caaggtttct tcgatattcc ggtggacaat
    12001 atttatgcca ccccgattct gttgaacgac atcaaacaac agcggattga aaatctgacc
    12061 gtcgtcagcc cggacatcgg cggtgtcgtc cgcgcccgcg ccgtggcaaa atccttgaat
    12121 gccgacttgg caatcatcga caaacgccgc ccgaaagcca atgtggcaga agtcatgaac
    12181 atcatcggcg atattcaagg cagaacctgt ctgattgtgg acgatatgat tgacactgca
    12241 aatacgctgt gcaaagccgc cgtcgctctg aaagagcggg gggcggaacg tgtcttggca
    12301 tatgccagcc acgccgtatt ctctggagag gcggtcagcc gtatcgcctc atccgaaatc
    12361 gaccaggtgg tcgtaaccga taccattcct ttgtctgaag cggctaaaaa ctgcgaccgt
    12421 atccgtcagg taacgattgc cggtctgttg gccgaaaccg tccgccgcat tagcaatgaa
    12481 gaatccgtct catatctttt caatgaagaa gtgatgacag gcagcatgtt gctgccataa
    12541 gcccgaagcc gtcttaagct ggtcgcggcc gatgacggcg attttaccta acttggagta
    12601 tttaacatga cttatgaaat tcaagcttct gttcgtgaag cacaaggcac tggtgcgagc
    12661 cgccgcctgc gtcgcgaagg ccaaatcccc ggcattctgt acggtgaagg tcaagagccc
    12721 gttgccattg ccgtggatca caaaaccgta ttctacgcat tggaaaaaga atctttccat
    12781 accgcgttga ttaaattgtc tctgaacggc gaaaccaaag acgttatcgt ccgtgatttc
    12841 caaatgcacc cgttccgccg cgaagtccaa cacatcgact tccaagccgt taaagccgat
    12901 cagcttgtac gcatccgtgt tcccctgcac atcgttaacg ctgaaaattc ccaagcggtc
    12961 aaactgcaag gcggtcgtgt atctctgttg aacacgacgg ttgaagtgct tgcgttgcct
    13021 gccaatatcc ctgctttctt ggatttggat tgtgccgaag tggttgccgg cgacattctg
    13081 cacttgtcag acatcaaact gcctgaaggt gtagaaagcg tttccctgaa acgtaacgaa
    13141 aatctggctg ttgctactgt taccggtaaa aaacgctaat tgattgaaaa gcaggcggta
    13201 cgtatatgat gcgtaccgcc ttattgtttt gggctgtgtt tcaatatttg gttgatttca
    13261 tccgtttata agggaaaacc gcttattttc gtttgggtag aaatctatct aggcgtattg
    13321 cgcgacaacg gatggttagc ttaaaaacgg catttcgtac cggatgaaaa tgaagaagat
    13381 atgtcgttca cattccggta gctgcccggc tggaaccggc tatcagagat agttggtcaa
    13441 ctttcaccct gaaaatcact tttcttttaa cttgcaacat attccgatag aatggacact
    13501 ttgtaagtaa tcattgatgg aagacgcaat tatggcaacc gaaccgaacc gaaccgaacc
    13561 gaaattatac acgatctaga aaataatttc aaacaaaaca acatattaaa actcaaacaa
    13621 ctctttcctg aagtcttttg cgaagaccaa atcgactttg agaagctcaa actcgttctc
    13681 ggcgcagaaa accttgccgg tgcgggcgaa cgttatcaac tcgattgggc gggtaaaacc
    13741 gccgcctatc tcaacctgca aagtcccact tcccgaaccc tcgttccctg caaagaagaa
    13801 tctgcaaatt tcgatggcac ccaaaacgtg tttatcgaag ccgaaaacct cgaagcactg
    13861 aaaatcctgc aaaaagccta cgccggcagc gtgaagatga tttacatcga cccgccctac
    13921 aataccggca gcgattcttt tatttatccc gacaaattct ccgaaagccg cgatgaatac
    13981 gcccgccgcg tgggcgatac cgacgacgcg ggctacctga agcgcgacgg cgtattccaa
    14041 ggcgcgtggc gcaaaaacgg caaagacagc ggccattacc acagcaactg gctctccatg
    14101 atgctgccgc gcctacatct ggcgaaaacc ctgctgcgcg aagacggcgt gatttttatc
    14161 agcattgacg acaacgaaca ggcgcagttg aaattgttat gtgatgaagt gtttggggcg
    14221 gagaattttg tcaatcaaat tgctgtcaaa atgagtgaac tttctggggt taaaatgaag
    14281 catttaaatc agtatgctaa attaaaagaa tttttactga tttacgctaa aaatattcat
    14341 ttcgctaatt tcaatattga aaagaaaaga aaatctcccg aaactctatc taaatattta
    14401 aaatattatt catcaataat agaaaatatt gagagtgaat gtgagcaatg gaagataata
    14461 tcactagatg aatattttaa agataaaaat atcattttag atagagagaa attgaatgac
    14521 tggaagctat caaatgcaca acgcctagtg tatcgtacaa acagtaaaac cgttgataaa
    14581 tttctactta aaaatccaaa tgctcctgat atttgtaaac tcatcaatga tgatggtaag
    14641 gaaattatca aatggggtaa caaagaaatg ttattcttgg aaaaatatat tgatgaatat
    14701 ttaggcgata tttggttgga tatatctact attaatttaa ataaagaaac tcatacctta
    14761 gtatttgaaa atggacaaaa acctttaaca ttgattaaaa gattactaaa aagtttagaa
    14821 actgaaaaca atattgttat ggacttcttc tccggcagcg gcaccaccgc ccatgccgtg
    14881 atgcaactga atgccgaaga cggcggcaac cgccgtttca tctgtgtcca gctccccgaa
    14941 gaaaccgacg aaaaatctga agcgcgcaag gcgggtttcg acacgattgc cgaaattgcc
    15001 aaagaacgca tccgccgcgc aggcaggcag atttcagacg gccttcaaga cggatcggaa
    15061 acagatacgg gctttaaagt tttcaaactt gccgaaagcg gtttcaaaca atggcggcag
    15121 cccgagcagt ccgatacgga agccctgcaa cgcgaattgt cgttgaatat cgattctgtg
    15181 ctgtccgaaa cgtcgtctga aaatctgctc tacgaattga tgctgcgtat gggcttgaag
    15241 ctgacttgca aggtttcgtt tttagacgac gtatatttcg tggaagacga ggatacgggc
    15301 gggctgtacg ctttcctgct gaaacgcgtg gatcaaggtt tgattgatgc cgtgctggcg
    15361 aagcatcccg ctaaggtggc ggcattggac aggctgtttg agggcgacga tgcgcttaaa
    15421 agcaataccg tattgcagat gaaggatgcg ggcgtgatgt tcgagtgtgt gtagtcagcc
    15481 gaaagcggga agtgccgtct gccgtcattc ccgcgcaggc gggaatctag aaactcaaag
    15541 ttgcaagaat ttatcggaaa tgactgaaac ccaacgaacc tagattccca ctttcgtggg
    15601 aatgacgata tatcagtttc tgcgccgaca ggtctggatt cccgcctgcg cgggaatgac
    15661 ggcaaagagg tagattttcc gcgcgacagc ggatagaagg aaataaaaat ggaactgaaa
    15721 tttgaagaac tgccctatca gcttgatgcg gtaaatgccg ttgcgaatct ttttgcaggg
    15781 cagccgaacc atgcccggac attcgatttg accagtcagg ggacggggcg gtttgtcggc
    15841 aacggcttgg atttggattg ggaaacgcta ggcctgaatc tgaacacggt gcaaaagcaa
    15901 aatggacagc ctgaaacgga aatcggcgcg cacgggctga atttttctct agaaatggaa
    15961 acgggtacgg gcaaaaccta tgtctatctg cgcacgattt acgaattaaa ccgcagttac
    16021 ggctggaaga aatttgttat tgtcgtaccg agcgtaccga tacgcgaagg cgtgttgaaa
    16081 agcctggaaa ttacgaaggc gcattttgac ggagtgttcg gcaggcccgt gatgcgttat
    16141 gcggaatatt caagcagccg gctatcggac ttgcgcactt ttgcggtaaa cgaccacatc
    16201 gagattttgg tgatcaatat ccagtcgttt gaaaaagatg gaaacgttat caataaggta
    16261 aacgaaagcg gggaaacgcc gattaagttt attcaggaaa cgtgtcctat cgttattatc
    16321 gacgagccgc agaatatgga aacggaaggc cgtctgaacg ctttggaatc gctcaatccg
    16381 ctctttaccc tgcgctattc ggcaacgcat aaacaaccgt accacaaggt ttacagcctg
    16441 aatcccgtgc aggcatacaa tttaaagctg gtaaagcaaa tcgaagtttt gccggtattg
    16501 gcggaaaacg atgtgaacgg cgcatttgtg gagctgctgg aagtaaagca ggcggccaaa
    16561 ggccgtccga aagccgattt gaacattcat tatcaggata aaaaggaaac caagaagaaa
    16621 aaggtatccg tcaaatggga agacgatttg tttgaaaaat cgggcggtaa cgaagcttac
    16681 cgccacggtt ttatcattga cggcatggat ttcgataagc aggaattggc gttctcaagc
    16741 ggtaaaacca ttacgcgcgg cggagacggc gatgcggtgc gcgatgaaat tatgaaaaac
    16801 caaatccgcg aaacggtaag ggagcatttg gcgaaggaga aacggctgaa tccgctgggg
    16861 attaaggtgt tgtctctgtt cttcatcgac cgtgtggaga attaccgtac agaagacgga
    16921 acggcaggca agtttgccct ttggtttgaa gaaatctatc gggaacttgc aggcaatacc
    16981 gatacggctg gcgtacacga cggctatttc tcgcaagaca ggaaaggccg tctgaaaaac
    17041 accgacggca cgacgcaggc ggacaacgat acctaccgcc tcattatgcg cgacaaggaa
    17101 accctgcttt cctttgacag cccgctgcgc tttatctttt cccattccgc actgaaagaa
    17161 ggctgggaca accccaacgt tttccaaatc tgcacgctca acgaaacccg ttcgccgctg
    17221 aaaaagcggc aggaaatcgg gcgcgggctg cgtatcgccg tcaatcagga aggacggcgc
    17281 gtgcgcgatg aaaacgtgaa catactgacc gttgtgccga acgaaagcta cgaattgttt
    17341 gccgccaatc tgcaaaagga atacgaagac gaatgcggta ttaaatttgc cgccgaaaac
    17401 atcaaaaacg gcaataagaa gaaaaaacag ttattccgca aagatttccc gctcgacccc
    17461 gaatttcagg caatttggga caagttgaaa tacaaaacgc gctatcgcgt gcgcttcgat
    17521 acggaagaat tgatcaaaca ggcttcggaa gcggtggcaa acctgccgga aatccaaaaa
    17581 ccgaaaatcc gcatccgcaa ggcgcaatta aaacaatcac aaacctacgg tatcgaaaca
    17641 gttgaaattg ccggccgcag ccgcgaaatg gacgttcaat gggagattcc ggacatactc
    17701 ggcgagattc aaagaaaaac ggggctgacg cggcgcacgg tgtacgaaat catcatacag
    17761 tcaggccgtc tgaaagatgt agccggtaat ccgcaacgct tcatcgacct taccgccgaa
    17821 gcggtaaacc gcagcctgca cgcgttgatg gcggcaggaa tcgagtatga gagaaccggc
    17881 gaagtgtacg accaaaagct gttggaagat atggcgcgcg aaggtatcga gttttacgaa
    17941 aacggtttta cattcacagt aagcaatccg caaaaaacca tttgcgaaaa ctacatccct
    18001 ttggattccg gcacggaaaa agcctttgcc caaaattgcg aggattatga tgcgaaaaat
    18061 gaaggaggcg tggcgtttta cttcaaactg cccggctggt tcaaaatccc gaccccgctg
    18121 ggcaattaca atcccgattg ggcggtggta aaacaggttc gggataaagt gtatttcgtt
    18181 gcggaaacca aaaacaccgg caaaggcatt cagaccggcg tggatatgga caagctgcgc
    18241 caaaatgaga ggctgaaaat caaatgcggc aagcggcatt ttgaagcgct gggcggtttg
    18301 gcataccgtg ttgtggaaaa agtgggggat ttaaagtcgg aaaaagagga gcaggcttaa
    18361 aacaaacagg tgtaatcgta gtatggcgat gttggattgg ggcggcggca accgaagatg
    18421 cggatatggt aatggctttg attgggtaaa ccgatcgggg actgaagatt gtttcagacg
    18481 gtctgttgat tttcttattt cggaaatggg aagatggaag gctattgccc tgtcagacgc
    18541 gtccaaagcc tttgccaccg gcttcttttt tctacatttt ccagtgcgac aatttctttt
    18601 tcggcaatgg tgtatccgtt ttgtctgatt ttgatttttc ctaggacctg cccttttttt
    18661 accggggcgg gaatcggctg tatggtttct agaatttgtt ctgccatttt cgcttcctta
    18721 tgtggcagag tgatgtaggc ttctttgagg aagcctgcgc ggacggtttt tttgctgcct
    18781 ccggaaattt ggatttgggc aacggttttg cctttcggat atattttggg cgtatcgaag
    18841 gcctgcaatg cccagttcag cagcttgctg ttgtctgatg cgcgtgtttc cgccgattcc
    18901 gaacccaatg tgatgacaag gatgtgcctg ccgttgccgg agtatgacac ggcaaggttg
    18961 tagccgccgc tttctgtgtg tccggctttc agaccgttta cattgttgtc cctatataaa
    19021 aggatattgc ggttgttttg ctctatattt ttgaatttga aagatttgat ggaaaacagc
    19081 gggtaatatt ccggaaagtc gcgcatcaat gcttcagaca gctgggcgag gtctttggcg
    19141 gtggaaacct gtccttctct actcaagcct gtcggatttt tgaatacagt gttcttcatg
    19201 cccaagcgtc gggcttcttt gttcatttgt tgcacaaaat tttcaatcga gccgttgccc
    19261 agccggccgg caagggttag ggcggcatcg tttgcggata gtgcaatcat gccttttaag
    19321 agtttgtcgg tgctgaccgt atcgccggga cgtacaaaca ttctgcttcc ttctgaagcc
    19381 catgcggatt cgggtatttt taagttttct tcagatcgga tatttcccga tttcatgttt
    19441 ttgaaaacca gatatgcggt catcagttgg gttagtgccg ccggttcgac aggggtattg
    19501 atgtttttgg cggataaaat ctgtttgctt tgaaggtcga taacgatgtg tgccgctgtg
    19561 agggtttcgg gtgtttggaa cgtgggggcg gcgtgtaccg ccggtttgtt gggcgcgggc
    19621 gatgcggccg ttgcgtgaga aacgcctaag atgatggaaa gcaggacggg caggatttta
    19681 tgtgctgtca tgaaatattc taattgtgtg cgtgtttcag tctgccgatt atacgcttag
    19741 ggtgtctgat cgggcggatt tttcttgctt tcgcgccgtc ttgggcgtat ggttttgggt
    19801 tttgcgattt taataaaccg attatcccat attgaattat gaacacgccc cttccttatt
    19861 ccaattacct catccgcatc ctgacggcat ctgtctatga tgtcgcggtc gaaacgcctt
    19921 tggaaccggc acgcagcctt tctgtacgtt tgaaaaacaa catccttttg aaacgcgaag
    19981 atttgcagcc ggttttttcg ttcaaaatac gcggcgcgta caacaaaatg tccaagttgc
    20041 cgaaagatgc gctcgcttgc ggcgtgattg cggcaagcgc gggcaatcac gcacaaggcg
    20101 tggcattgtc cgcacagcgt ttgggttgcc gtgccgttat cgtcatgccg gagactacgc
    20161 cgaaaatcaa agtggatgcg gtcaaaagcc gtggcggcga ggtggttttg cgcggtgttt
    20221 catacaacga tgcttacgat tatgcgatgg agctggcgga acaggaaggg ctgacctata
    20281 tcgcaccgtt tgacgatcct gatgtgattg caggacaagg gacggtaggg atggaaattg
    20341 tcagccagca tcccgatcca atccgcgccg tattcgtacc gataggcggt ggcggtttgg
    20401 cggcgggcgt ggcggcattt atcaagcagg tccgccccga aatcaaagtt atcggcgttc
    20461 agaccaacga ttcctgctgt atgaagcagt cggtcgaagc gggtgaaatc gtccatttga
    20521 aagatgtcgg gctgttttca gacggcactg cggtcaaagt cgtcggaaac gaaaccttcc
    20581 gcctctgcaa agaacttttg gatgaaatca ttacagtcga taccgatgcg gtttgcggcg
    20641 cggtcaagga tattttcgat gacacgcgca gcattaccga gccggcgggt gcattggcat
    20701 tggcaggtct gaaagcctat atcgcccgag aaggcgcgga aaaccaaacc ctgattgccg
    20761 ttaccagcgg cgcgaatatg aattttcacc gtttgcgcca cgtttcggaa cggagcgaat
    20821 tgggcgaggg caacgaaggt atttttgccg ttaccatccc cgaagagcgc ggcagcttcc
    20881 ttaagtttgt caatatattg ggaaatagga atattaccga gttcaactac cgctacgggg
    20941 acgatgaaaa agcgcatatc tttgtcggac ttcaagcggc aggcccgcag gatttggcgg
    21001 ttatcggcag ccggttggat gaggcgggat tgcccaatgt cgacctgacc gatgatgaga
    21061 ttgccaaaat ccatatccgc tatatggtcg gagggcggac ggacaaagta gaaaacgagc
    21121 gtctggtcag ttttgagttt ccggagcgtc cgggggcgtt ggcgcgtttt ttgaaccata
    21181 tgcagggcgg ttggaacatt acgctcttcc attaccgcaa ccacggtgcg gattacgggc
    21241 ggattttggt cggtatcgat gtgccgccgc acgatgccgc cgcatttgac ggtttcttgg
    21301 aaagtctggg atacagctat cacgaggaaa cgcaaaatgc cgcgtacaag ctgtttcttg
    21361 cctgacgctt gaaagcacaa tgccgtctga aagcctttca gacggcattg cgctttcatg
    21421 gttaaatcga atattcaatc agttcgtttt gagagaagac ataaacctgt ttcggaatca
    21481 gcttcaattc tttgccttcg gcgattgggt aacgcgcggc atcgctgcca gccagcgtga
    21541 tatgcacgtc ttgtttgccg tgtttcacca aaatatgcgt caatgccccg acggcgtgga
    21601 ctttttcgat ttcggcacaa atcatcggtg tttcgtgttc ggcggcgatc tgccattcgt
    21661 gcgggcggat atagccggtg gcggtttgtt cctgccattt gtattgtgcg tccaatttcc
    21721 acgcgaagcc gttataatgc cagaagcctt tttcgatgcg tccttcaaaa gcgtcggttt
    21781 cgccgaggaa ctcggtaacg aaggcatttt cgggtttgcg gtaaatagct tcggcgctgc
    21841 cggtttgttc gattttgccg tggttcatca cgacgatttc gtcggaaact tcgagggctt
    21901 cttcttggtc gtgcgtaacc agaatgctgg ttacacccag gttgtgatgg atgtcgcgca
    21961 gccaggtgcg taattctttg cgtactttgg catccaacgc gccgaagggt tcgtccaaaa
    22021 gcaggagttt cggttcgacc gcaagcgcac gggcgagggc gatgcgctgg cgttgcccgc
    22081 cggagagttg gtgcggatag gattttgcca aatgcgagag ttgtacgagt ttcagcaatt
    22141 cttcgacttt ggcgcggatt tgtcctttgg acgggcgttc ggacttgggc aatacggtca
    22201 aaccgaaggc aacgttgtca aacacgttca tatggcggaa aagggcgtag tgttggaaca
    22261 cgaagccgac tttgcgctcg cgcacgtgct tggcggttac gtcttgccca tcaaacagaa
    22321 tcttgccgcc gtcggcgttt tccagtccgg cgataatgcg taaaagcgtg gttttgccgc
    22381 agccggacgg gccgagcagg gaaacgagtt tgccggtggg gacgttgagg ttgatgtttt
    22441 tcagcgcgtg aaaattgccg aagtgtttgt ttaagttttg gatggtgata ctcatactgc
    22501 gttcctttcg gcggcggcga gttttttgtc ttgtaatttg gtaatgatgt tctgcaccgc
    22561 cagcgtcgcc agtgccaaaa gtgccaatac gccggagagg gcgaatgcgc cggtgaagtt
    22621 gtattcgttg tagaagattt cgaccaaaag cgggacggtg ttggtttcgc cgcgtatgtg
    22681 tcccgatacc acgctgaccg cgccgaactc gcccatcgcg cgggcgttgg tgaggatgat
    22741 gccgtagagt aacgcccatt tgatgttggg cagggtaacg cgccaaaaca tctgccagcc
    22801 gcttgcgccg agtatcaatg ccgcctgttc ttcgctgtcg ccctgagcct gcatcagcgg
    22861 gatgatttcg cgtgcgacaa aggggaaagt aacgaacagc gtcgccagaa taatgccggg
    22921 gatggcgaag ataatctgta tgccttgcgc ttcgagccag ccacccaatg ccgtgtgcgc
    22981 gccgaacaat aagacgaaca ttaaaccggc caccacgggc gatacggaaa acggcaaatc
    23041 gagcagggta gtcagcaact gcttgccgcg aaaatcaaaa cgggtcagca gccacgccat
    23101 cgccacgccc aagacggcat tgacggggac gacaatcagc gcggtaatca gcgtcaattt
    23161 gatggcagac cacgcttcgg gatcgtttaa ggatttcagg tacaaatccc aaccgccttt
    23221 taaggcttcg taaaacacgg cgacgagcgg cacgaccagc atcagcagca gaaagcccag
    23281 cgcggcggca atcagcaaca cgcgcagcca gcgcggttcg gtcaggttgg gattggcgga
    23341 atagggtttc atggcggtgg tgttccccgg ttggttgttt taaggtgttc ggatgccgtc
    23401 tgaaaaaccg ttttttgtgg ggcggattcg ttttcagaca accttttatt gattaaggga
    23461 ataaaaacgt ctagctaccg tcattcccgc gcaggcggga atccaccgca gggcaacagg
    23521 aaaacagaaa ataaataagg cagccgaaat tcaccaatgg attcccgcct gcgcgggaat
    23581 gacggtaaca ggtatttcag acgacctcaa cccttcgcgc ccgaacgcct gcccaacgcc
    23641 cactgcatca cgttcagcgc aaacagaatc acaaacgaaa ccagcagcat aaacaacgcc
    23701 accgccgacg cgccctgcac gtcgaactgt tccagcttgc ccgtaataat cagcggcagg
    23761 atttcagaaa ccatcggaat attgcccgcg ataaaaatca ccgagccgta ttcccccgtt
    23821 gcccgcgcga acatcattcc cgcgccggtc aagagtgccg gcgtgatttc cggcagcagc
    23881 acgcggcgga acgtcgtaaa gcggtttgcg cccaaagtcg ccgccgcttc ctcatattcg
    23941 cccgataatt cctccaacac cggctgcacg gcgcggacga taaagggcag gctgacgacg
    24001 accagcgcaa tccaaatgcc gacgggcgta aacgcgattt tgatgcccaa aggctcgaaa
    24061 aaacggccta tccaaccgtt gggcgcatac agggttgcca acgcgatacc cgtaaccgcc
    24121 gtcggcagcg caaacggcaa atcgaccagc gcgttcgcca gacccttgcc cgggaattca
    24181 taacgcacca atacccacgc caccagcgtg ccgaacacga cattggtcag catcgcataa
    24241 aacgacatcc gcaaactcag ccacaccgcc gccaacacgt tcggctcggc aatcgtgttc
    24301 caaaagccgc cccagccgat ttccgccgcc ttcgccgcca tcatcgcaaa cggcaagacc
    24361 acaagcagcg acaggcacaa tacggtcaga ccaaggctga gtttgaagcc gggcagtacg
    24421 ccgggcgttt tgagcggtaa cataaaacaa tgctgaaaat aaggaaaagg aaggactact
    24481 ttaacgatgc cgtccgaaaa acggaaagaa tggaaagttt ggtgcaaaga cgaatttgtt
    24541 ataaagcggt tggcagtttc tcaagcgggc gcgatgtttt aaaatatagt ggattaaatt
    24601 taaatcagga caaggcgacg aagccgcaga cagtacaaat agtacggcaa ggcgaggcaa
    24661 cgccgtactg gtttaaattt aatccactat aacgccttgt tttgacggaa aaccatcata
    24721 taaaggaaca cttatgcaga ttttatcttt tcagccggac attgcggaac gtatgctgga
    24781 aggtacggaa ggcgagtcgg tcaacgaaaa cgcacaattc gtccgtacgg acaacggcta
    24841 ttggattgcg tggcatgaag gcgtagcggc actgcttgcg cccgatacgc cgccgggcat
    24901 tccctgtttt tgggtggaag gcgcggaaag ccttgaagag ttgtgcgtca tggtggaacg
    24961 cggcgagttt gacgaagtgg aagagtttga cggcgatgac gacgaatggc tcgaaacggc
    25021 acagggttgc gggcaccacg gcgacgcttg cgcctgcgga cattaaaggc attgcaggct
    25081 tgccgcaagg ggggcaaggc tttgccgttt tttaaataat cggtggattg catctgtttg
    25141 caatgggctg aacgcttccc tttgtatttt attgaataaa aaaacttatc ttggtattat
    25201 aattaaggca gcatcaatta ttttgggatg gcaataaacg caaagcattg atttgcgccg
    25261 attgcagact tattatagca ggttgcggcg cggacttaat gggaattgtt tatagagcat
    25321 gatggtctga aacatcatta attaaaaata ccattaatcc gatttatatt tatttcaatt
    25381 tcaatggaaa aacatcaatg acaatgattt taagcatttt aagcctgttt tttatcatca
    25441 gactgttatt tttagccgtc tctattaaac atgaaaaagc cttgattgcc aaaggggcga
    25501 aacaatacgg aaaaaccaat tccacgctgc ttgcggcagt tcatacgctt tattatttgg
    25561 cgtgttttgt ttgggtatgg ctttctgaca ctgcttttaa tggcatatcc ttgattggta
    25621 cgctgacggt gatggcttcg tttgtgatat tgtcattgat tattaagcag ttgggggaga
    25681 tttggacggt taaaatctat attttaccaa atcatcaaat taatcgttcg tggttgttta
    25741 aaacattccg ccaccccaat tattttttaa acatcatacc cgaactgatt ggcatcgcct
    25801 tattatgtca agcgtggtat gttttattga ttggcctgcc catttatttg ctggtcttat
    25861 ttaagcgtat ccgacaagaa gaacaggcga tggcaacact tttttaaccc gtttcatcaa
    25921 ttatag
//
LOCUS       DAWFLT010000022        25149 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWFLT010000022 DAWFLT010000000
VERSION     DAWFLT010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25149
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.022"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(1046..2947)
                     /gene="thiC"
                     /locus_tag="WGR74_000934"
     CDS             complement(1046..2947)
                     /gene="thiC"
                     /locus_tag="WGR74_000934"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ0905376.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGAEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLNE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKSGSNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKSEYAKLLKQHAGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
     regulatory      complement(3038..3137)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3287..3363
                     /locus_tag="WGR74_000935"
     tRNA            3287..3363
                     /locus_tag="WGR74_000935"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3321..3323,aa:Met,seq:cat)
     gene            3545..4420
                     /locus_tag="WGR74_000936"
     CDS             3545..4420
                     /locus_tag="WGR74_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ0905377.1"
                     /translation="MPTVHFTESVSKQDLDALFEWAKASYGAKSCWKTLYLNRLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWSDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNILFSLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGRRSPHKAVDPDK
                     LDNIAAGGVSSGELPSEAVCRESSEEAGLDQTLFPLIRPVSQLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVACFEKMDIGSLVAAMLSENMMHDAQLVTLDAFCRYGLI
                     DVAHPLSEWLDGIRL"
     gene            4429..5367
                     /locus_tag="WGR74_000937"
     CDS             4429..5367
                     /locus_tag="WGR74_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0905378.1"
                     /translation="MLELNGLCKRFGGKTVADDICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIQLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEVHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRGTLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVARLMGLPNTDDDRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(6087..7862)
                     /gene="ptsP"
                     /locus_tag="WGR74_000938"
     CDS             complement(6087..7862)
                     /gene="ptsP"
                     /locus_tag="WGR74_000938"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ0905379.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAAH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIVKVIRCEDE
                     EKSEKLIKQMNSVSVEEEADFKGRK"
     gene            complement(7862..8131)
                     /locus_tag="WGR74_000939"
     CDS             complement(7862..8131)
                     /locus_tag="WGR74_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ0905380.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            complement(8200..8637)
                     /locus_tag="WGR74_000940"
     CDS             complement(8200..8637)
                     /locus_tag="WGR74_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ0905381.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(8718..9281)
                     /locus_tag="WGR74_000941"
     CDS             complement(8718..9281)
                     /locus_tag="WGR74_000941"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ0905382.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            complement(9350..10174)
                     /locus_tag="WGR74_000942"
     CDS             complement(9350..10174)
                     /locus_tag="WGR74_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ0905383.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            10265..10897
                     /locus_tag="WGR74_000943"
     CDS             10265..10897
                     /locus_tag="WGR74_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ0905384.1"
                     /translation="MTLRYEILSVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFALPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(11103..12887)
                     /gene="rmuC"
                     /locus_tag="WGR74_000944"
     CDS             complement(11103..12887)
                     /gene="rmuC"
                     /locus_tag="WGR74_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ0905385.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFADLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLADVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     QGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKANKQ
                     LARDLTERANETTALSESLEYVAEDEAV"
     gene            complement(13226..14014)
                     /locus_tag="WGR74_000945"
     CDS             complement(13226..14014)
                     /locus_tag="WGR74_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ0905386.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            complement(14029..15378)
                     /locus_tag="WGR74_000946"
     CDS             complement(14029..15378)
                     /locus_tag="WGR74_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ0905387.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDVLGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            complement(15397..15978)
                     /gene="petA"
                     /locus_tag="WGR74_000947"
     CDS             complement(15397..15978)
                     /gene="petA"
                     /locus_tag="WGR74_000947"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ0905388.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(16101..16850)
                     /locus_tag="WGR74_000948"
     CDS             complement(16101..16850)
                     /locus_tag="WGR74_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ0905389.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            16981..17910
                     /locus_tag="WGR74_000949"
     CDS             16981..17910
                     /locus_tag="WGR74_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0905390.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITDDGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELESV"
     gene            complement(18165..18557)
                     /gene="rpsI"
                     /locus_tag="WGR74_000950"
     CDS             complement(18165..18557)
                     /gene="rpsI"
                     /locus_tag="WGR74_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ0905391.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(18567..18998)
                     /gene="rplM"
                     /locus_tag="WGR74_000951"
     CDS             complement(18567..18998)
                     /gene="rplM"
                     /locus_tag="WGR74_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ0905392.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(19176..19355)
                     /gene="ssrS"
                     /locus_tag="WGR74_000952"
     ncRNA           complement(19176..19355)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WGR74_000952"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(19361..19666)
                     /locus_tag="WGR74_000953"
     CDS             complement(19361..19666)
                     /locus_tag="WGR74_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ0905393.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(19677..20006)
                     /locus_tag="WGR74_000954"
     CDS             complement(19677..20006)
                     /locus_tag="WGR74_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905394.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QRQETQQ"
     gene            complement(20061..21050)
                     /locus_tag="WGR74_000955"
     CDS             complement(20061..21050)
                     /locus_tag="WGR74_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ0905395.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(21173..23875)
                     /gene="ppc"
                     /locus_tag="WGR74_000956"
     CDS             complement(21173..23875)
                     /gene="ppc"
                     /locus_tag="WGR74_000956"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ0905396.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKTDGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRNMEHDFQMVYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHVDVVAELFQHAGLEDYNRLNEEQKQTALLRELSHQRPLYSP
                     FITYSDHTRRELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAAVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            23918..24811
                     /locus_tag="WGR74_000957"
     CDS             23918..24811
                     /locus_tag="WGR74_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ0905397.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDELLLRYSRHILLDEIGIEGQQKLSDAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADFDTVELHNLQRQVAFDEGDVGKLKTEALAGRLKRINHTVNVRAVNEKLDGCRL
                     TGLVQAADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPVCGTAR"
BASE COUNT         5451 a         6778 c         6701 g         6219 t
ORIGIN      
        1 ttcggtttcg cttgttttaa gtttcgggta acttccactt cgtcattccc gcgcaggcgg
       61 gaatccagtg cgttgagttt cagctattta gaataaattt tgaaacttta accccgtcat
      121 tcccacgaaa gtgggaattc agaacgtaaa atctcaagaa accgttttac ccgataagtt
      181 tctgtgccga cagacctaga ttcccgcctg cgcgggaatg acgggatttg aggttgcggc
      241 atttatcggg agcaacagaa tccgctctgc cgtcattccc acgaaagtgg gaatctagga
      301 cgcaaaatct caagaaatcg ttttgcccga taagtttccg caccgacaga cctagattcc
      361 cgcctgcgcg ggaatgacga atccatccat acggaaacct gcaccacgtc attcctacga
      421 acctacattc cgtcattcct acgaaagtgg gaattcagaa cgtaaaatct caagaaaccg
      481 ttttacccga taagtttctg tgccgacaga cctagattcc cgcctgcgcg ggaatgacgg
      541 gatttgagat tgcggcattt atcgggagca acagaatccg ctctgccgtc attcccacga
      601 aagtgggaat ccaggacgca aaatctcaag aaaccgtttt gcccgataag tttccgcacc
      661 gacagaccta gattcccgcc tgcgtgggaa tgacggcgga aagattctat ttttcccgat
      721 aatcacccac aatctcaaat tccttcattc tctcaaaaac aaaatcagaa ccctaaatcc
      781 catcatcccc atctatgtga atataaaaat tttaaaaatt atagtggatt aacaaaaacc
      841 agtacggcgt tgcctcgcct tagctcaaag agaacgattc tctaaggtgc tcaagcacca
      901 agtgaatcgg ttccgtacta tctgtactgt ctgtggcttc gtcgccttgt cctgattttt
      961 gttaatccac tatattttca caagcgaaag aatgccgtct gaagcctttt ttccgggttt
     1021 cagacggcat tttttgcttg acgtttcaac tgtaaatctt cgcgcctttt ttgacgaact
     1081 cgaccgcttt ttcctccatg ccctgccgtt gggctttttg cttgtcggcg tagtcgcgca
     1141 cttcctgcgt gattttcatc gagcagaatt tggggccgca cattgagcag aagtgggcga
     1201 ttttcgcgcc ttcggcaggc agggtttcgt cgtggaagct ttcggcacgt tcagggtcga
     1261 ggcttaagcg aaattggtcg cgccaacgga actcgaaacg cgccttgctc agggcgttgt
     1321 cacgtaattg tgcgcccggc cagcctttgg cgagatcggc ggcgtgggcg gcgagtttgt
     1381 aggtgatgat gccggtgcgc acgtcttctt tgtcgggcag ccccaaatgt tctttggggg
     1441 taacgtaaca aagcatcgcc gtgccgtacc agccgatatt ggccgcgcct atgcccgagg
     1501 tgatgtggtc gtagccgggt gcgatgtcgg taacgagcgg gccgagtgtg taaaaaggtg
     1561 cttcaaagca gtgttgcagc tcttcggtca tgttttcttt gacgcgttgc agcggcacat
     1621 ggccggggcc ttcgatcatg acttgtacgt catgtttcca cgccttatcg gtcaattcgc
     1681 ccaaggtgtg cagctcggcg aattgggatt cgtcgttggc atcggcaatg cagccggggc
     1741 gcaggccgtc gccgaggctg aacgacacat catacgcttt catgatttcg cagatttcgt
     1801 cgaaatgcgt gtagaggaag ttttcccgat gatgtgccaa acaccatttc gccataatcg
     1861 aaccgccgcg cgagacgatg ccggtgaggc ggttggcggt catcggtaca taacgcagca
     1921 acacgcccgc gtgtatggtg aaatagtcca cgccttgttc cgcctgctcg attaaggtgt
     1981 cgcggaacaa atcccaagtc aaatcttcgg caatgccgcc ggttttttcc aaagcctgat
     2041 aaataggcac ggtgccgatg gggacgggcg cgttgcggat aatccattcg cgcgtttcat
     2101 ggatgtgcgc gcctgtggat aagtccataa ttgtgtccgc gccccaacgc agcgaccaca
     2161 ccattttttc gacttcttcg gtcaggctgg aagtgacggc ggaattgccc aagttgccgt
     2221 tgattttgac gcggaagttg cggccgataa tcatcggttc gagttcggga tggttgatgt
     2281 tggcggggat aatcgcgcgt ccggcggcga tttcttggcg cacgaattcg ggcgtgattt
     2341 ggtcgggacg ggttgggatg ttcgcgccga aactttgccc tgcgtgttgt ttcaagagtt
     2401 tggcgtattc cgatttttgg gacaattcgt ctaattttaa acgttcgcgt atggcgacaa
     2461 actccatttc gggcgtgata atgccttggc gcgcgtagtg aagctgggtt acgttgctac
     2521 cgcttttcgc gcggcgcggg cgggtgattt ggttgaagcg cagatgggcg gttttcggat
     2581 cgtgtgcgcg ttcgatgccg tattcgctgg agagcttggg caggatttcg gtatcgccgc
     2641 gttcgttcag ccatgcggtg cggatgtgcg gcagaccttg tttcaggtcg atgtgtgccg
     2701 ctggatcgcc gtacacgccg ctggtgtcat agacgggaat cggcgggttg gcttccgcgc
     2761 cttgcgccgt ataggtgtcg tcctgacgga tttcacgcaa aggcacgcgg atgtcgtcgc
     2821 ggctgccttg cagatacacg cgttccgagt tcggatattt gaagcggata ccgatgtctt
     2881 cgctcaagtc ggcaagttcg cgcgcttcgt tgccggaagt tttggcggtt ttttttggcg
     2941 tagtcataaa aaatgctcct gttttctcgt ttagaataaa gaaacaggag cgttttgcgt
     3001 tttcagacgg catttgaaaa ccaatgctgt ctgaaagaca gaatccgtga aaactcccca
     3061 cgcaggtatt atcccgatcg ggtgtaaagg gtatttctca gccgcctgaa catcatgcag
     3121 cacccctgtt tcaatgttaa ccaaaattaa atcacgaaca tgaacttttg taaagaaaat
     3181 gatatttcaa atcaggcata aaccgccgga cgacaaaatt ttatgatttt tcgcggaaat
     3241 aatgtttgac aacataaaaa atctgccgta tagtttcgtt ttctgacgcg ggatggagca
     3301 gcatggtagc tcgtcgggct cataacccga aggtcgtagg ttcgaatcct gctcccgcaa
     3361 ccaaatatca aacccctcgg ttcaatgccg aggggttttg ttttgcctgt ttcctgccgc
     3421 ctccgttttt tgccggattt tccttcctgc cgcaatatcg gaacagcagc ccgccgtctg
     3481 tttgcggttg caaattcagg cggtttgatt acaatcttcc gcattatgtt caagaaagcc
     3541 gactatgccg accgtccatt ttaccgaatc cgtcagcaaa caagaccttg atgccctgtt
     3601 cgagtgggca aaagcaagtt acggtgcaaa aagttgctgg aaaacgctgt atctgaaccg
     3661 tctgcctttg ggcaatctgt cgccggaatg ggcggaacgc gtcaaaaaag actgggaggc
     3721 aggctgctcg gagtcttcag acggcatttt cctgaatgcg gacggctggt cagatatggg
     3781 cggccgcttg cagcacctcg cccgcacatg gaacaaggcg gggctgcttc acggatggcg
     3841 caacgagtgt ttcgacctga ccgacggcgg cggcaatatc ttgttctcac tcgaacgcgc
     3901 cgctttccgt ccgttcggac tgctcagccg cgccgtccat ctcaacggtc tgactgaatc
     3961 ggatggcaga tggcattttt ggataggcag gcgcagtccg cacaaagcag tcgatcccga
     4021 caaactcgac aatattgccg ccggcggtgt ttccagcggt gaattgccgt ctgaagccgt
     4081 gtgtcgcgaa agcagcgaag aagcgggttt ggatcaaacg ctgtttccgc tcatccgccc
     4141 ggtatcgcag ctgcacagcc tgcgccccgt cagccggggt gtgcacaatg aaatcctgta
     4201 tgtgttcgat gccgtcctgc ccgaaacctt cctgcctgaa aatcaggatg gcgaggttgc
     4261 gtgttttgag aaaatggaca tcggcagcct ggttgccgcc atgttgtcgg aaaacatgat
     4321 gcacgacgcg caactggtta cgctggacgc gttttgccgt tacggtctga ttgatgtcgc
     4381 ccatccgctg tccgagtggc tggacggcat acgtttatag gatgcgccat gcttgaactg
     4441 aacggactct gcaaacgctt cggcggcaaa acggttgccg acgatatctg cctgactgtc
     4501 gggcgcggca aaatactcgc cgtactgggg cggtcgggct gcggaaaatc caccctgctg
     4561 aatatgattg cgggcatcgt ccggccggac ggcggcgaaa ttcagctgaa cggggaaaac
     4621 attacccgta tgccgcccga aaaacgccgt atttcgctga tgtttcaaga ttacgcgctg
     4681 tttccccata tgagtgcgct ggaaaatgcg gcattcggtt tgaaaatgca aaaaatgccg
     4741 aaagccgaag ccgaacgcct tgccatggcg gcacttgccg aagtcggact ggaaaacgag
     4801 gtgcaccgca agcctgaaaa actttccgga ggcgagaaac aacggttggc actggcgcgc
     4861 gctttggttg tccgcccttc cctgctgctg ttggacgaat cgttttccag tttggacacg
     4921 catttgcgcg gcacgctgcg ccgcatgact gccgaacgca tccgcaaggg cggcatccct
     4981 gccgttttgg taacgcattc gcccgaagag gcctgcacgg cggcggacga aatcgccgtc
     5041 atgcacgagg ggaaaatcct tcaatgcggt acgcccgaaa ccttggttca aacgcctgcc
     5101 ggcgtgcagg tcgcccgact gatgggtttg cccaataccg acgatgaccg ccatattccg
     5161 caacatgcgg tgcgtttcga ccaagacggc atggagtgcc gcgtattatc ccgtacctgt
     5221 ttgcccgaat cgttcagcct gtccgtcctc catccggaac acggcatcct gtggctgaac
     5281 ctcgatatgc cgcacgccgg tgaaatatcg ggaaacgata cggtacgcat ccatatcgaa
     5341 gacagggaaa tcgtccgctt ccgctgatgc ttcttaaaaa caaaatgccg tctgaaaacc
     5401 tttcagacgg cattttttac caaagcagcc atatttttta tcagggctgc aaaatttatc
     5461 cgaaacaaca gaaaccaccc tgccgtcatt cccgcaaaag cgggaatcta gaacccaaca
     5521 cggcaaaaat ttatccgaaa caacaaaaat cttttcatcg tcattcccgc aaaagcggga
     5581 atctagaacg taaaatctaa agaaaccgtt tttcccgata agtttccgtg ccgacagacc
     5641 tagattcccg cctgcgcggg aatgacggga ttttaggttt ctgatttcgg ttttctgttt
     5701 taagggaatg acgagacttg agatggcggc atttatcggg agcaacagaa accaccctgc
     5761 cgtcattccc gcaaaagcgg gaatctagaa cccaacacgg caaaaattta tccgaagcga
     5821 caacaatctt ttcatcgtca ttcccgcgca ggcgggaatc tagaacgtaa aatctcaaga
     5881 aaccgtttta cccgataagt ttccgcaccg acagacctag attcccgcct gcgcgggaat
     5941 gacggatttt aggtttctga ttttggtttt ctgtttttga gggaatggcc gattttgggt
     6001 ttctgtttcg gttttctatt ttgcaagaat ggcaaaattt cagattgcgg gcattgttaa
     6061 gtatttctat tttttacctg ccgtatttat ttccgcccct tgaagtcggc ttcttcctcg
     6121 acagacacgc tgttcatctg tttgatcagc ttttccgact tctcttcgtc ttcgcagcgg
     6181 atgactttca caatatcgct ttcgagctgt ccgacattgc tgtgcagaat gatgtttttg
     6241 acgggcagga tgttgttggg gttcatggaa aaacggcgca gccccatacc caataaaacg
     6301 cgggtaaacg cggtatcgcc cgccatctcg ccgcatacgg atacgtcttt gtccatgcgg
     6361 ttggcggtac ggatgacgtg ttgcagcatt ttcagcacgg cgggatggcc gggctggtag
     6421 aggtggctga cgctgtcgtc gccgcgatcg acggacaaga tgtattgaat caggtcgttg
     6481 gtaccgacgg agatgaaatc gaccagtttc aaaatactgc cgacggtcag cgcggcagac
     6541 ggaatttcaa tcatacagcc gatgccgact ttaccgaagg catcgccgcg ttcggcaagc
     6601 tggcgttgcg cggtgtcgag gtggatgagg cactggcgca cttcggatac ggaggtaatc
     6661 atcggccaca tcatccgcac ggggccgtgg gcggcggcgc ggaggatggc gcgcatctgg
     6721 gtgcggaaca tgaccggttc ggcaaggcac aggcggatgc cggtcatgcc cagcgcgggg
     6781 ttgaggctgc cgttgggcgt gctgtttttc ccgaaccagc gcgggttttt gtccacaccc
     6841 aaatcgactg tccgtatcgt tacgcttttg cctttcattt ttttgacaat cgcgctgtac
     6901 acttcgtact gctcgtcttc agacggcatc gtatcgcggt tcaggtaaag aaactcgctg
     6961 cggaacagcc cgatgccgtc tgcgccgagg ttgtgcagcg gtttcacgtc ttcggcggat
     7021 tctatattgc ccacaagctc gatgcagacc ccgtcagcgg tggcggcggc ggtttttttg
     7081 agcttgttca aatcgcgttt gtggctgcgg tattcgcggg cacggcggcg gtattcgttc
     7141 aacaccgact catccggcgc gataatcaac acgccgttga taccgtccac aatgaccgtt
     7201 tcgccctcgg taatcagttt gcgcgcgttg tgcagcccga cgacggacgg gatgtccaag
     7261 ctcctgccca aaatcgccgt atgcccggtg gggccgccgg catcggtaac gaaggcggca
     7321 atgcgctgct ctttaaacaa aaccgtgtcg gcgggcgaaa ggtcgtttgc aatcagaacg
     7381 gtttcgtcaa acaggttgtc ggcaacttcc aactcgttgc cctgcccgat caggttgttg
     7441 tggatgcggc ggacgacttg cagcatatcc tgcttgcgtt cgcgcaaata ggcatcgtcc
     7501 atattgtcga attgggcggc gagtttgtcg ctctgctgct tcaatgccca ctcggcgttg
     7561 attttttgtt cccttaaaat atcgacgggt tcgcgcgaca aggtaacatc ggtcaagagc
     7621 atcaggtgca gcgagatgaa cgcgcccaac tcggtcgggg cgttttcggg aatcgcgctg
     7681 cggagctgtt ccaactcttt gcgcgtggct ttgacggcgg catcgaaacg ttcggcttcg
     7741 gcatcggtgt ccgcctccgc aacatcatac tgcggcactt cctccgtacc gcgcgcaatc
     7801 aggtgggcgc aaccgacggc aatgcctttg cccgccgcca cgccgtgcag cacgatactc
     7861 attattcgcc ctcgccgaag tagccgttga ttaagtcggt cagggcgcgc atcgcttccg
     7921 cctcgtccgc gccgtccgtc tccagttcga tgaccgtacc cttggcggcg gcgagcatca
     7981 tcagccccat aatgcttttg ccgttgacgc ggctgtcgtt tttcgtaacc cagacttcgc
     8041 ttttgaattg ggacgcggtt tgggtgaact tgttggacgc gcgggcgtgg agtccgagtt
     8101 tgttgatgat ttcgatggat tgtttgagca tttcgattcc cgtgttatgt atatcggcag
     8161 cagacgccgt ttaaaatgtt ttcctgccct gccgcttctt cagacggcat cgccgctgcg
     8221 ccggcacacc aaatcttcgg gcgcggacgt gatggcgaaa atgcctttta ccgccgcctc
     8281 cctgacgcat tcggtaaagg cggcaaggtc ttccgccgcc ggcgaatatt ggacggcctt
     8341 aaccatcatc ggcgcgttca gcccggtcaa aatcgccgat ttgttttcgc gcacgaggcg
     8401 gcgggctgca ttgcaggggg tcgcgccgaa aatatcggtc ataattagca cgccgtcgtt
     8461 gtcgggaaac tcctgaagcg cggcaatggc gttgttgatg atgtcgtctt ggtcttccgt
     8521 cggttgcacg ccgagtatgc ggacgttttc aggcagtccg cccggaaaaa aatgatgtgc
     8581 cagcttgcgg taggcttcgc ctatggtttc gtgtgtgatg attaaaagcc ctatcatatt
     8641 atgcgtcctg ttcctcgttg tcctaccggc gtatgggcgc gatgccgtct gaaaggcctt
     8701 cagacggcat cgcgtcctta ttttccgtcc aatgcgtaaa tctcgcccag attgcgccag
     8761 cagcccgccg catccatgcc gtaaccgaaa acataacggt tcggcacatc cagtccgaca
     8821 taatcggctc ggataggctt gggtttgtca atcagcttgt tggcgaacac cgccgcacgg
     8881 cagcttgccg cacccatttc caaaagtttg gcttgaatgg cggacatcgt atgcccttcg
     8941 tccaaaatat cgtccagcac gacgacgtgc ctgccccgga tttgttccgc atcgggcata
     9001 cgcttccagt tgaacgcgcc gccctccagc ttgtcgccgt aacgggaaac gtgaacataa
     9061 tcaaaatcta agggaaaacg caacagcggc agcaactgcc ccgtaaacac caccgcgccg
     9121 cccatcacgg gcagcagcag cggatatttg ccgcccaaat cgcgcgtaat ctcgtccgcc
     9181 actttttgca gtgcggcacg gcattggcct tggtcgaaca aaagatcagc gttttcaagc
     9241 atcgcctgtg tttcaaggcg tttggtttct aaatcggtca tatgtcggaa tcggtcggta
     9301 aaaggaaaat tataaaccaa agtatcggat gccgtctgaa ctgtcctgct cagcggtcgg
     9361 tacgcacgcg cacaaatgtg gcaaatttcg gcgtgccttt ccgcgtaaag ccacggtaac
     9421 ggtaggtaat cagcgtgccg attttgggcg ggttgtcgcg gtctttgtcc ttgaaaccgc
     9481 tgccgatgcg gaattcgccg tgtcggtttt tgcagccgac cgcgcccagc cgtccggcgt
     9541 ttcgcccttt gccctcatag tgccgcgtta ccgtgcattc gtcgtcgtat tggcttttca
     9601 gcttcaataa ttggctgctc ctgccgccgc tataacggga ttcgggctgg cgcagcatca
     9661 cgccttcgcc gccctgcgct tcgatttgtt ttaaaaagtc catcgcgtgc tgccggtcgc
     9721 gcactttgat ttgcgggatg atggtaatcg gcgcgttcgg atgcgttttc agccactgcg
     9781 ttgcgactgc caaacgttgg tagagattgc cctgcgcctt gggtacgtcg aaaacgtgca
     9841 gacggatgcc gcgccagtct gaagaaacag aacgcacggt agcggaaatc tgctcgaact
     9901 gaccgcgtcc gctatacaat tcgccgtcca aaggataagg cggaaactgc gcggtaaagc
     9961 ctttgggggg cgtaaacgcg tagccctgac ggcttatcag gtgctttccg tcccaatagg
    10021 cgcgcacgcc gtcgagtttc tcgctcattg cccagccggc aatatcctgc cctttgtatt
    10081 cctgcgccag catcaaatcc gccgcgcctg ccgatgcagg gatgaaaacc gccgtaaaaa
    10141 tcggtatgat gccgccgatt gtcttcttaa tcatctgatt ccccaaatat caaaacaggc
    10201 ggcaaaccgc cataaaacaa acggcaaacc cgatgccgtc tgaaaaaccg tttaggaaca
    10261 cgccatgacc ctacgttacg aaatcctctc cgttaccccc ttccgccaaa actgcaccct
    10321 gatttgggac gacgaaagcg gcgaagccgt cctgaccgat gtcggcggcg acgtgccgtt
    10381 cctgctgcaa gcgttggcaa accgcaaact tacgctcacg gcaatctggc tgacgcacgg
    10441 ccatctcgat cacgcgggcg gcgtggtcga aatgttgaaa acgcataaag tccctgtcct
    10501 cgggccgcat ccggacgatg aattcctgct ccaatcgctg ccgcaaacca ccgcgcaata
    10561 cggatttcct gtctcgcccg cctttgcgcc gaaccgttgg ctcgaagaag gcgaaacgct
    10621 cacggtcgga cgctatgcct ttcaagtgct gcatattccg ggccatacgc cgggacatat
    10681 cgtcttttat tgtgccgagg cggaattgct gattgcgggc gacgtgctgt tttacgaaac
    10741 cataggcaga accgattttc cgcgcggcaa ccacgccgac ttaatcaata atatccgcaa
    10801 caaattattc gccctccccg aaaccgtgca agttgtcgcc ggacacgggc gtatgacttc
    10861 catcggacac gaaaaacggc acaatccgtt tttctaaccg acttcactac ggtcttcaga
    10921 cggcgtcatc tgcactgatg ccgtctgaaa cgcaaaaggc tcagacaacc gccgccttgc
    10981 cggtgtacct cgccggacaa ggctttggta agtacttcaa acaaacccca aatcagaacc
    11041 ggtacagtta cgccgcgctt tcggcattcc cgcccctgct gaaacaagct tttccgcaca
    11101 agtcagactg cttcatcttc tgccacatat tccaaagatt ccgacaacgc cgttgtttca
    11161 ttggcacgtt cggtcaaatc gcgtgcaagc tgcttgtttg ccttcacgcc caacagacgc
    11221 agtttctcgg cgcgtccgac cagattcccg cgcccctcgg caagttgctt aaatgccgtc
    11281 tgaaagctgt tttgcgcctg atcgatgcct ttgccgacgc tttcgagcgt ctgtacaaag
    11341 ccgacaaatt tgtcgtacag cttgccgcct tcgtccgcaa tcgccagcgc gttttggttt
    11401 tgctgctcgt tgcgccaaat attcgccacc gtccgcaaag tcgccagcag cgtactgggg
    11461 ccgaccagca taatccgttt gtcgaaacac tcttggaaca agccagcgtc attctgcaac
    11521 gccaacaggt aggccggttc gacagggata aacataaaga cgaaatccaa tgtgttcaca
    11581 ccttccaaat cggtgtaatc cttcagcgac aagcctttca tatgcgcgcg tatgctggcg
    11641 acatgcgccg ccaactcgcg tgccgccgta tccgcatccg ccgcctgcgt gtagcgcaca
    11701 taagctgtca gcgagacctt ggagtcaatc acaatctgct tgttgtcggg caggttgacc
    11761 aaaacgtcgg gctggaggcg gcgcgtgccg ccgtcttcct cttttcggac ggatgctgcc
    11821 tgaaccacat attcccgccc tttctgaagg ccggaatttt ccaaaaccgt ttccagaatc
    11881 atctcgcccc aattgccctg aaccttattc tgcgtaccgg tcagcgcgtt ggtcagggcc
    11941 tttgcctcgc tgtgcagctg cgcattcaat ccttgaagcc gtttcaattc gttttccaac
    12001 gtcagccgct cgcgcgattc tttatcatag gtttgcttga ccaactcgcc gaaaccttgg
    12061 atgcgttcgt ttagcgggtt caaaacctga tggagctgct cccggttctg ctcggtaaaa
    12121 cggcggcttt tttcttccaa aatcgtgttg gcaagatttt gaaactgatc gctcaaactt
    12181 ttgcgcgcct cgcccagcaa ggacagcttc tcttcagaag caaggcgttc ctgttcgatt
    12241 tgcgttgcca aacgttcgtt ttcaaccgcc aaaccctgtg ccttttcctg caactcggta
    12301 tgcgactgcc tcaaccgctc cgcttccgcc tctttttcct gcaaatgggc aatctgtttt
    12361 tcggctgcgg caaaacggtt gccgacatcg gcaaggtcgt tttgcacgtc gcggacagtt
    12421 tggcggcttt cttccaaatc ggtttcgatt ctttggcgga tttggcgttc caaggcatat
    12481 tggtcggcaa acgccttccg ttcctgtccg agctgcgttg ccaaacgttc gttttcaacc
    12541 gccaaaccct gtgccttttc ctgcaactcg atatacgact gcttcagccg aaccgattcc
    12601 gcctcttttt cctgcaaatg ggcaatctgc ttttcggctg cggaaaaacg gttgcccaaa
    12661 gcataatttt cgtcctgcaa atgtcggtat ttcccgtcca aaacggccaa ttccgatgca
    12721 atttctgcgt gtgcctgttc gacaaaatca catcttgccg ccttttccgc cagttgcgcg
    12781 ttcaaatcgg caaactcgcc ctgaaacctg cccttcatca gcaaccatgt aaacaacacg
    12841 cccgacacca acgccgccaa aggcagcaaa acagtcatca gttccatcaa tcatcctaat
    12901 attcaaacat tttcacaccg gacaaaaccg ccgcttattc cgattctacc tgtttgttcg
    12961 acatagctcc aaaaaatata gcggattggc tttaaacctg ttcgacatcg ccttaccatg
    13021 ctgcttgcgg tttcagacct tttcctaatt caatatcaat ctgccacaaa ccctgattaa
    13081 gttcccgatg tctgacattt ttagaatgat gccgtctgaa atgttgcagc tatgttcaga
    13141 cggcatacgg attcaggctt ttcaaacggc aggcaaaatg aaaaaagggc aaaccctaaa
    13201 ggatttgccc ttttgttcca aacgcttagt gtacgtcttt ccaatattct tttttcagga
    13261 aatacgccaa aggcagcata accgcaaata ggaaaatcat cacgacatag cctatacgtt
    13321 tgcgttgcag ttgtgcaggt tcgcccatgt acacaaggta attgaccaaa tcgcgtacat
    13381 atgcgtcgta ctctttttgg atcactttgc cgttaggcag acggcggctg tgcaaaccgg
    13441 tagattccca atacagctta ggcttcatct cgccgtgttc gtcttttacc ataaccggct
    13501 gacctttggc atccaactca acggcttgaa cgccttgctg ttcccacaac gggtggggca
    13561 taccgacttt atcgaataca gtattgttcc agccgctcgg acgggtcgga tctttataga
    13621 agccgcgcat ataagcgtaa aggtagtctg cacctttgga acgcgcaatc aatgtcaaat
    13681 cgggcggagc agcaccaaac cattttgccg catctttcgg gttcatcgcc gaatgcatga
    13741 catcgccgac attatcggtg gtaaacatca ggtttttctt gatttcttcg tcagtcaaac
    13801 cgatgtcttt caaacggttg aaacgcatac cgcttgccga gtggcaggac aggcaatagt
    13861 tggtaaagat ttgtgcgccg cgctgcaggc tgacttggtc gcgcaaatcg atatcgactt
    13921 tctcatagtg tgcatgaccg cccgaagcaa atgccgcacc catgggtact gccaacagca
    13981 aggcagcaaa ccaatttttc atagcttgtt tcattttgac tgccctcatc agatgttggt
    14041 tgcaaacagg taagcaccca cggcggtaat gccgacgtaa acaaagaaca tgattttttg
    14101 tttggcggtg ctcatggtta cgcgttcggg aaccggtttg tttttatcca gcttggtata
    14161 gaacggcata cccaggaaga atgcgaaata gacgaaggac aggatgcgcg cgaccaaagt
    14221 acgcgtatcg gttgccacca tcgcgcccaa aatacccaaa ccgataaagg aaatgatgaa
    14281 caagaccaat gcggttttga aaatcgggcc gcggtaacgg acggatttca cctcgcccct
    14341 atccagccaa ggcagcaggg caatcaacac gaccgccgcg cccataccga ttacacccca
    14401 aacctgcgtt cccaagaacg agggaatcgc acgcaaaatg gcgtagaacg gtgtgaaata
    14461 ccataccggc gcaatgtgcg gaggtgtttt cagcgcattt gccgcatcga agttcggcgc
    14521 ttcgaggaag tagccgccgc cttccggtgc gaagaacaat acggcacaga agacaatcag
    14581 gaatacaacc acacccaaga cgtctttaac ggtatagtaa ggatggaatg ggataccgtc
    14641 gcgcgggata ccgttttcgt ctttgttttt cttgatttca acgccgtcag ggttgttaga
    14701 acccacttcg tgcagggcga tgatgtgcgc cacaaccaag ccgagcaata ccagaggtac
    14761 cgcgataacg tgcagggcaa agaagcggtt cagggtaacg tcggaaacgt tgaagtcgcc
    14821 gcgaatccaa gtggacaaat ccggaccgat aacagggatg gcggagaaca ggttaataat
    14881 tacctgcgca ccccaaaagg acatctgacc ccaaggcagc aggtagccca taaaggcttc
    14941 cgccatcaat gccaagaaaa tcagagaacc gaaaatccac accaactcac ggggtttttt
    15001 gtacgaaccg taaatcaaac cacggaacat gtgcagataa acaacaatga agaagaaaga
    15061 tgcgccggtg gaatgcatgt agcggataat ccaaccgccg gacacgtcgc gcatgatgta
    15121 ctcgactgca gtaaaggcag caggcagatg gtaggcgttc aggttgccgt cgggtttgta
    15181 gttcatggtc aggaaaatgc cgctgacgat ttgaatcacg aggacaagca gggccaaaga
    15241 gccgaagtag taccaaaaat taaagttttt cggtgcgtag tattgagcca aatgctcatt
    15301 ccacatttta gacaggggga aacgagcgtc catccagcct aacaatgctt ttgctttgct
    15361 attggtttgg tttgccataa ttatcgttcc ttattcttag tcttcgccca ccaagatagt
    15421 tgtgtcgctc aagtatttat atggcgggac aaccaggttg gtcggggcag gaacaccttt
    15481 atatacgcgg ccggccaagt cgaatttcga accgtggcag gggcagaaga agccgccttt
    15541 ccagtctgca cccaaatcgg caggggcaat gtcgggacgg aaggtgggcg agcagcccaa
    15601 atgggtgcag ataccgatgg cgacaaggat gttcggctta atcgaacggg tctcgttttt
    15661 agcatactcc ggctgctgtt ccgcatcgga attgggatcg gtaagttcgc cgttcaggcc
    15721 tttcaggtct ttaagctgct gatctgtacg gttgagcacc caaatcggtt tgccttgcca
    15781 ctcggcggtc agcaactgac ccgcttcgat tttactgaca tccacctcga cggcagcacc
    15841 ggcggccttg gctttttccg aagggaaaaa actggccaca aacggcgttg ccacacccaa
    15901 tgctgccact ccgcccgcgc cgcaggtcgc gagtgtcagg aaacggcggc ggccgttgtt
    15961 gatttcttga ttatccatta ttcagtcgtc ctaatatttt gggaataccg agccattaaa
    16021 cgttgcaatt ttacccagtt tgcagtgata ctcaaagcat tatttaaaat aaggtaaagt
    16081 tttatgatat ttctcaagac tcaagccgga ttgttttcgt caaaatggca cacttccaac
    16141 ccgaaaacct ctgccgccga ttctgccagc gcgcgtacgc cgtaacgttc cgtcgcgtga
    16201 tgccctgccg aaatgaaagc cgtacccgtt tcattggcaa ggtggtattg agcttcagag
    16261 atttcccccg tcaaatacag atcgacacct tcgtctattg ccgtctgaaa aaacccctgc
    16321 gccccgccgc tacaccatgc aacccgtcgg atttcgcgtt cgggtttgcc gatgacgaca
    16381 ggcttgcgtt gcaaaactgt ttcaatatgc gccgccaatg cgccgagtgt cttggcttgt
    16441 ttcaggctgc ccgagttgag caggttttgt tcgccgaacc gtttttctgt cgcaaaaccc
    16501 aatctgtcgg cgagttgggc attgttgccc agtgtgggat gtgcatccag gggcagatgg
    16561 tagcctgcca tattgatgtc gtgccgtaac agtgcggcaa tccgttcttt tttccaacca
    16621 gtaacggtcg gcaactcgtt tttccagaac ataccgtgat gtaccaaaag caaatctgcc
    16681 ttctgctcca cagcaaaatc aatcgctgcc ctgcttgccg ttaccgacgt aacgattttc
    16741 ccgatatatt ccctcccttc aacctgcaaa ccgttagggg cgtaatcttt aaacaacgct
    16801 gtctgcaatg tttcattaca ccaagtcaga aaatccctgc gcaataccat cttttttcct
    16861 aatcgcttta aacaagcggg cattctaatc gcaaaatgtc cggaattcac atttttccga
    16921 tttgcacccg catatgaatt attttaatat gcgccggttc aatatgccgt ctgaagcccc
    16981 atggattcca ttatcgaatt gcgccacctc aaaaccctgc tggcacttga agaaaccggc
    17041 agcgtctccc ttgccgccaa acgggttttc cttacccaat ccgccctttc ccaccagatc
    17101 cgtatgctcg aaaaccacta cggcacgccg ctgttcgaac gcaaatccac gcccttgcgc
    17161 tttaccccgg tgggcgaaag gctgctgcgc ctcgcccacg aactgatacc tcaagttgct
    17221 gttgcagaac gagatttggc gcgaatcaca gaaggggagg cgggagagct gcggattgcc
    17281 gtcgaatgcc atacctgttt cgactggctg atgcccgcca tgggcgaatt ccgcccgatg
    17341 tggccccaag tcgaattgga tatcgtatcg ggattccaag cggatcccgt cggactgctg
    17401 ctgcaacacc gtgccgacct tgccattgtt tccgaagcgg aaaaacaaaa cggtatttct
    17461 ttccaaccgc tgtttgccta cgaaatggtc ggcatttgcg caccagacca tccgcttgcc
    17521 gccaaaaacg tttggacggc ggaagacttt atcggggaaa ccctgattac ttatcccgtt
    17581 cccgacgaga tgctggattt gcccaaaaaa atcctgattc cgaaaaacat caacccgccg
    17641 cgccgacaca gcgaactgac catcgccatt atccaactgg ttgccagcag acgtggcatt
    17701 gccgcccttc cctattggac agtcatgccc taccttgaaa aaggctatgt cgttcaccgg
    17761 cagattaccg acgacggact gcaaagcaaa ctgtatgctg ccatccgcac tgaagatacg
    17821 gacaagagct atctgaacaa cttttgccaa atcatacgtg aacgcggttt tgcagatttg
    17881 cccggactga gcgaactgga atcggtctga ccccttattc aaccataccc ggcagttttt
    17941 ctattttttc atgtatagtg gattaacaaa aaccagtaca gcgttgcctc gccttgccat
    18001 actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactatact
    18061 gtttttgatt tttgcccaat ctgtaatctt tagattgcca atggggaacc gtctactaca
    18121 aataaaaaac cctgcgataa gcagggtttt ttgaatttcc aacattaacg tttggagaat
    18181 tgttttgcac ggcgtgcttt gcgcagaccc ggttttttac gttcgacttc gcgggcatcg
    18241 cgggtaacaa aaccagcttg agacaaggcg ggtttcaacg cggcatcgaa gtcgatcagg
    18301 gcacgggtaa tgccgtggcg gattgcgccg gactggccgg tttcgccgcc gccaacaaca
    18361 ttgactttga tgtcgaaaga ttcggcgttt tcagtcagaa ccaagggttg gcgaacaacc
    18421 attcggctgg tttcccgtgc gaagaattcg tcaacgggac gaccgtttac gatgatttga
    18481 cctgtacctt taatcaggaa tacacgagcc actgaacttt tgcggcggcc tgtgccgtag
    18541 tagtatttac cgttcatgtc gcgtccttat ttcagttcca aaactttggg ttgttgcgca
    18601 gcatgggcgt gttccgcacc cgcatacact ttcagttttt taatcatggc gtaacccaga
    18661 ggacctttgg gcagcatacc ttttacagct tgttccaaag cgcggcccgg gaattgctct
    18721 tgcatttcgc ggaaggtgcg ttcgtagata ccgcctggga aaccggaatg gcggaagtat
    18781 tttttatctt cgaatttggc accggttaca cgcagtttgt ccgcattgat aacaatgatg
    18841 taatcgccgg tatcgacgtg gggggtgtat tcaggtttgt gtttgccacg cagacggctg
    18901 gcgacttcgg ccgcaacgcg acccaagact ttgtcttggg catcgatgac gaaccattcg
    18961 cgcttcacct cgtggggttt cgctgaaaag gttttcatag tggaaatcca gatagatata
    19021 gaaagttgta aattttaaag acaggattcg attttgtcaa tcgcattacc gcgttacgga
    19081 aggattttcc ggatttcggc agactgcata ctgctttttc gggcggggcg gcggccaatg
    19141 tgaaaaaccg catcgttgcg atgcggtttt gaatgggaat ccccgcgaga gccgtttcgg
    19201 ccgaatccgc ttgaaccttg ctgacaaggc ggctgcctcg ggtagtttcg ggtgcgtccg
    19261 caaaaggacg ctcgcgccca ctactgctcc cggcaacctt aagcgaactt attggttcaa
    19321 aggaatatat gccttcgcgg acaccgcagg gaaaaagggg ttattcctgc gccaagcggg
    19381 atagtgcttt ttggcaggcg ttgtccatat cggctatttt acgcgcaaaa tcgccgattg
    19441 ccaaatcgcc gccgttcagg gaggttttca acaggtcgtg gacgacgttg agcgcggcca
    19501 taatgacgat tttttcgctg tccgcgacgc gtccgccttc gcggatggct tcggctttgc
    19561 cgttgagcat tccgactgcc tgcaacagtg tgtctttttc ttctgccggc gtgttgacgg
    19621 tcagccgggc gtgcatgact tcgatgtgga cttgttcgat gttcatcctt taatccttat
    19681 tgctgcgttt cctgccgttg ggggaggcgc gctgccagtg cgctgatttt ttccctgctc
    19741 tgttcgagca ggctgcggta tcgtgtattt tcttctgtca ggctgtcaat tttgttttgc
    19801 aggtcttctt tgagtttgcc gacttggacg agcagggctt cgctaagttc gtcgacggcg
    19861 gtttcgtgtt cgagtttttg ccgctcgtgc gcccgtttga gttcggcgac ggtttctttg
    19921 aggcggcggt tttcgctgac gagggtttcg aatttttgta ccagcgtata aacgctgctt
    19981 tcgagttttt cgatattttg tttcataacc ttacctgtcc gtatgccgtc tgaaggcttc
    20041 agacggcatc tgtctgttgt ttattcaaaa cgcgcgctac gttccatcag cctttcgacg
    20101 acctgctgag gggtcatttc cttacggatg agttgcagca gggtttgggt aatcggcata
    20161 tcgatttggt acttacaggc agtattgaag acttcttcta tcgtgctgac cccttcggaa
    20221 acgtgtccga tttcgaccag cacctgatgc agttccttgc cttctgccaa acccaagccg
    20281 acgcggcggt tgcgtgagag tgcgccagtg caggtcaaga tgaggtcgcc gatgcctgcc
    20341 agccccatca tggttttggg ctgtgcgccc attgcggagg caaggcgggt aatttcagct
    20401 aatccgcgcg taaccagtgc ggcacgggcg ttaagcccgt actctaggcc atcggacaat
    20461 ccggtggcaa tcgccataac attttttacc gcgccgccaa ccgccacgcc gataacgtcg
    20521 gtactgccgt aaagcctcat gacagtcgtg ttgagctgcg gtacgagttc ttcaatccac
    20581 tcttggtttt cggaggcgag gacgacggca cagggcagtt gtttggcgag ttcctgtgca
    20641 aaactcgggc cggagagtac gccgattttc ttattgtcgg gcaatacttc tttcaatact
    20701 tgaaaggtca gcagccctgt atcctgctcg aatcctttgc aggcggcgag gacggggagg
    20761 tgtcccgcgc cgtactgttt gagcagctct gcgctgcttc tcaatccggc aacggaggtt
    20821 acgataagga caagtccgct gtctttgagc gcgtctgcca aatccgcaca cacttccaag
    20881 gtttcgggaa aggaaaagcc gggcagtccg cgtttgtttt cacgcgcttc ctgcatttgg
    20941 cggacttggt ctgcgttgcg cgtccacagg gatacgcggt tgccgtgttg ggaaaagtgc
    21001 agggcgagcg ccgtacccca cgaacctgcg ccgataacgg taattttcat tggtcgtctt
    21061 tcaacatatc actgccgttc actttaaaac aatcggtgtt tctctgcaag tgcggtcagg
    21121 gaaatgccgt ctgaaaggtg ttcagacggc attttgcccc gatgcggcac aatcagcctg
    21181 tattgcgcaa accttgcgcc acgccgttga tggtcaggtg aaccatcagc agcgcgtgcg
    21241 gattgtcggg ttctttacgc aggcgtttga gcatggcgac ctgcaggccg ttgagcgcgt
    21301 tcaggtaggg aatcctcaag gcaagcgaac gggcgaggct gcggttgtcg cgtaaaagtt
    21361 cttcggtttg cagcaggtcg agcagggctt tgcggctgcg ttggtattct tccttaatca
    21421 tgccgaagat gacggcggct ttttccggcg attcgctcaa gccggcatag ttttccgcga
    21481 gggtgatgtc ggttttcgcc atcacttgct ccatattgga aagcatggct tggaagaacg
    21541 ggttgctttg ggcgtgttcg cgcagggcgg cgagcgtttc gggtttgtct tcgcacaagg
    21601 tttccaccgc gctgccgaaa ccgtaccaag caggcagcat gaggcggttt tgcatccagg
    21661 aaaataccca cggaatcgcg cgcaagtctt gaatccgcgc taaggtcttg cggctggcag
    21721 gacggctgcc gaggttgagg gtggcgattt cctgaatcgg gctggtttgc aggaagtagt
    21781 cgatgaagtc ggggtgggta atcagttcgc ggtagtattt gaacgatacg tccgacaatg
    21841 cctgcatcag tttggcatca gggtcttttt tatccggcag gatgctggct tccaaagtcg
    21901 cggcaaccaa ggtttccaag ttgcgttggg cattgccggg gtcggcgtat ttggcggtaa
    21961 tgacttcgcc ttgttcggta atgcggattt gtcccgccac gctgcccgcc ggttgggcga
    22021 gaatggcttg gtaagacggg ccgccgccgc gtccgacgct gccgccgcga ccgtggaaca
    22081 ggcgcatgcg gacatcgtat tttttgaaga gttcgaccaa gcccaattcc gcctgataca
    22141 gacaccatga gctggtaaca tagccgccgt ctttgttgga gtcggagtag ccgagcatga
    22201 tttcttggat gtttccgcgg ctttcgagca gtgcgtcgta ccaatcgagg cggaacatgg
    22261 tttccatgac cggacaggcg ttttcgagtg cttcgatggt ttcaaacagc ggcacgatat
    22321 tgatgcggct gtgcggtttg ccgttttcca ccgccaacag gccgctttct ttcagcagca
    22381 atgccaaggc gagcaggtcg ctgggttgtt cgcagttgga aataatgctt tgtgtaacgg
    22441 catcttcgcc gaattcgtct ttgattttgc gcgcttcgtt gaaaatcgcc agttcgcggc
    22501 gggtatggtc gctgtatgtg ataaacgggc tgtacagcgg acgttgatgg ctcaattcgc
    22561 gcaacagggc ggtttgtttt tgctcttcgt tcaggcggtt gtagtcttcc aagcctgcgt
    22621 gttggaaaag ctcggcaacc acatcgacgt gtttgcctgc gtgttggcgc aggtcgagcg
    22681 gcatcatgtg gaagccgaac acggacacgg agcggatgag gtctgccaaa cggccttcag
    22741 caagcaggcg gctgccgttg tcgataaggg aacgttgcaa ttttttcaaa tcatcgagaa
    22801 attcttgtgc cgaagcatac ggctcgagaa agccgaattt gcagcccatg cccaaaccga
    22861 gcgcgcgcgc tttgcccata gcgcgcgcca tgatgtaagc aatggcgcgg cggtagggtt
    22921 cttcggtgcg agcgatttct tcgtcaggcg agagggctgc cagtgccatt acgtcgtcgt
    22981 tgactttgac gcggcggatg gagagcggca gttcgcggta aagtttgtcg agttcgccac
    23041 ggtaaaaacg gaacacggca tcggcgtggc ggcggaaggc aaaacgcaag gtttcggcag
    23101 aaacgaacgg attgccgtcg cggtcgccgc caatccagcc accgattttg aggatattcg
    23161 gaacgcagac atcgggatag accatctgaa agtcgtgttc catattgcgg tagagcttgg
    23221 gcagggcttc gaaaaagctc atcgggaaga tggacacgcc gttgttgatt tcgtcgttga
    23281 cgctgagttt gtggcggcgc gtttcgctgg tctgccacaa gcccagcagg atagtgtcga
    23341 tttcgcggcg cagccgtgcc agcgcgtcgg cattggtgca gcgttcgcgt tgcggcaaca
    23401 gtgcgcggat gcggcggttg aagcttaaga cggtttggcg ttgcacttcg gtcgggtgcg
    23461 cggtcaaaac ggcggtaacg gacgtattgt ccaactgccg ctgcaccgat ttgccgtcgg
    23521 ttttccccgc tttgagcctg cggacggttt ccgtcaggct gccttccgcg ccgccgcgtc
    23581 cggcttcttc gtggatttgg cggcggcgtt cgtggtgcac gtcttcggcg atgttcaaaa
    23641 tctgggcgaa caggccgcag gccaaggtta aatcgtgggt ttgttgttcg tccaattgcg
    23701 gcaatacttt ttcaatcaat gccgcgctgt cgtcggaagt ggacaagagt ttgaccgttt
    23761 cgacaaccaa cggcgaggct tcttcgtgca ggaggttgaa cagggattgt ttcagaaatt
    23821 ccgcgtccgc cgccaaagcc gcgtccttcg gattgttcag aatatgcagt tgcatgattt
    23881 ttctctcatt gccgtaaata ctgtaaatgt acctcaaatg ccgcatccgt gccaaaccgt
    23941 tcacacttta accgcccgtg tcccgaaatg ccgtctgaag ttgaacgccg cccgacggca
    24001 gcgttacaat cgcccgcaac tgttttttcc gaacatcatc atgaccacga ccgaacacga
    24061 caacgacgac gaactcctgc tgcggtacag ccgccacatc ctcttggacg aaatcggcat
    24121 cgaagggcag caaaagcttt ccgacgcgca tattttggtc gtcggatgcg gcggtttggg
    24181 tgccgccgca ctgccctacc ttgccgcttc gggtgtcggc acgctgacca tagccgattt
    24241 cgacacggtc gaactgcaca acctgcaacg ccaagtcgca tttgacgagg gcgatgtcgg
    24301 caaactcaaa accgaagcct tggcaggccg cctgaaacgc atcaaccata ccgtcaacgt
    24361 ccgcgccgtc aacgaaaaac tcgacggctg ccgcctgacc ggtttggttc aagccgccga
    24421 catcgtttta gactgttgcg acaactacgc cacgcggcaa gccgtcaacc gtgcctgcgt
    24481 gcaaacgaaa acaccgctgg tttcaggggc ggcggtacgc tttgaagggc aacttgccgt
    24541 gtaccgtccc gacttgcccg actcgccgtg ttacgcctgc ctgtttgacg gcggatcggc
    24601 ttcagacggc atctgttctc tcttcggcgt gttctcgccg ctggtcggca tcatcggcag
    24661 tacccaagcg gcggaggctc tgaaaatcct gctggatgcg ggcgaaccgt cgcacggcag
    24721 gctggcggtt taccgtgcct tggaaggggg ctggcaatat ttcgacctgc cgcgcaaccc
    24781 tgaatgcccg gtttgcggca cggcgcgata aaccctgccg ccgtttcaga cggcatccaa
    24841 acggatgcgg cggaacggtt ttaaaaattt aaaaatttac atttctttgc aaaaaaaaaa
    24901 aaaaaaaaaa ataaacttac cttataattg caattgtttt agcaatgtct gtttcgcaga
    24961 ctcattgagt aaaacgtttt ccccgtaatg tgtttggccg tctgtcccct ttgggttcgg
    25021 acggcttttt tttggctgtg tttgaatacc cggttggttt tatctgtttg cagcggggga
    25081 agccgcttat ttccgttcgg gcggaaaacg gttccatcgg ataaaaggca ttttgtccga
    25141 ctgattaaa
//
LOCUS       DAWFLT010000023        23438 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWFLT010000023 DAWFLT010000000
VERSION     DAWFLT010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23438
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.023"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(429..1571)
                     /locus_tag="WGR74_000958"
     CDS             complement(429..1571)
                     /locus_tag="WGR74_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0905398.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASVANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LTPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
     gene            1875..2138
                     /gene="rpsT"
                     /locus_tag="WGR74_000959"
     CDS             1875..2138
                     /gene="rpsT"
                     /locus_tag="WGR74_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ0905399.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            2298..3446
                     /locus_tag="WGR74_000960"
     CDS             2298..3446
                     /locus_tag="WGR74_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ0905400.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            3589..4290
                     /locus_tag="WGR74_000961"
     CDS             3589..4290
                     /locus_tag="WGR74_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ0905401.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPQ
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            4348..6156
                     /gene="aspS"
                     /locus_tag="WGR74_000962"
     CDS             4348..6156
                     /gene="aspS"
                     /locus_tag="WGR74_000962"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ0905402.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSSRNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALGVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIARTGAE
                     NGDIIFFGADKAKVVNEAIGALRIKVGLEHGAENGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKVAETKEA"
     gene            6307..6591
                     /locus_tag="WGR74_000963"
     CDS             6307..6591
                     /locus_tag="WGR74_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0905403.1"
                     /translation="MKKLLLAAVVSLSAATAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            6758..8650
                     /gene="speA"
                     /locus_tag="WGR74_000964"
     CDS             6758..8650
                     /gene="speA"
                     /locus_tag="WGR74_000964"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ0905404.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPAQHNQTASLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            8749..9672
                     /gene="speB"
                     /locus_tag="WGR74_000965"
     CDS             8749..9672
                     /gene="speB"
                     /locus_tag="WGR74_000965"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ0905405.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESQADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            9870..11336
                     /locus_tag="WGR74_000966"
     CDS             9870..11336
                     /locus_tag="WGR74_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ0905406.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFLAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(11543..12640)
                     /locus_tag="WGR74_000967"
     CDS             complement(11543..12640)
                     /locus_tag="WGR74_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ0905407.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGKGNLRLGN"
     gene            12863..14005
                     /gene="bioF"
                     /locus_tag="WGR74_000968"
     CDS             12863..14005
                     /gene="bioF"
                     /locus_tag="WGR74_000968"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ0905408.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENCKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNIYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRQEVAHRTQIMPSQTCIVPYILGGNEAALAK
                     AEYLQRQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAACL"
     gene            14015..14662
                     /locus_tag="WGR74_000969"
     CDS             14015..14662
                     /locus_tag="WGR74_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ0905409.1"
                     /translation="MKTKFYDCQGEHLILYFAGWGTPPDAVNHLILPTNHDLLICYDY
                     QDLRLDFDFSSYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFDEIHQELTALFAMIGQDRR
                     TDLICWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            14650..15432
                     /gene="bioC"
                     /locus_tag="WGR74_000970"
     CDS             14650..15432
                     /gene="bioC"
                     /locus_tag="WGR74_000970"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ0905410.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMTHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCNVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFITHCKTGLKTNGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQAWLAKDFELLWCEDFTVILDFDTPSDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFGMPSGKVRLTYHPLFFIARYSAAGRQ"
     gene            15652..17088
                     /locus_tag="WGR74_000971"
     CDS             15652..17088
                     /locus_tag="WGR74_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ0905411.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQYADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            17091..18188
                     /locus_tag="WGR74_000972"
     CDS             17091..18188
                     /locus_tag="WGR74_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ0905412.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            18200..18736
                     /locus_tag="WGR74_000973"
     CDS             18200..18736
                     /locus_tag="WGR74_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ0905413.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            18738..19382
                     /locus_tag="WGR74_000974"
     CDS             18738..19382
                     /locus_tag="WGR74_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ0905414.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            19405..19764
                     /locus_tag="WGR74_000975"
     CDS             19405..19764
                     /locus_tag="WGR74_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ0905415.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            19857..19932
                     /locus_tag="WGR74_000976"
     tRNA            19857..19932
                     /locus_tag="WGR74_000976"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19890..19892,aa:Asn,seq:gtt)
     gene            complement(20431..20826)
                     /locus_tag="WGR74_000977"
     CDS             complement(20431..20826)
                     /locus_tag="WGR74_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905416.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAGGGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDPETDKFAKVSG"
     gene            complement(20810..21127)
                     /locus_tag="WGR74_000978"
     CDS             complement(20810..21127)
                     /locus_tag="WGR74_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ0905417.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMAVIAQSEFVEFAKINKS
                     ENVIDCFSFFWNRRIKHDIWLISFSDNSEMVIKESLKDGHKIYKFEFCEIVDNCNFDD
                     VFV"
     gene            complement(21255..21533)
                     /locus_tag="WGR74_000979"
     CDS             complement(21255..21533)
                     /locus_tag="WGR74_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905418.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSLKDNLQSGLETGWIVFGVMASVYFVSLLKKVLK"
     gene            complement(21540..21824)
                     /locus_tag="WGR74_000980"
     CDS             complement(21540..21824)
                     /locus_tag="WGR74_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905419.1"
                     /translation="MFGGRLKNAPSITAKHFLKENIMKFINTCRKYGAKLAVVTAAPL
                     ALAAHANATLPDTAKNALEAAKADGMEVGWIVVGIFAALFVFSIVKRVMK"
     gene            complement(21832..22029)
                     /locus_tag="WGR74_000981"
     CDS             complement(21832..22029)
                     /locus_tag="WGR74_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905420.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            complement(22034..22324)
                     /locus_tag="WGR74_000982"
     CDS             complement(22034..22324)
                     /locus_tag="WGR74_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905421.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            complement(22369..23438)
                     /locus_tag="WGR74_000983"
                     /pseudo
     CDS             complement(22369..23438)
                     /locus_tag="WGR74_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
BASE COUNT         5893 a         5454 c         5931 g         6160 t
ORIGIN      
        1 aatgctaagg caatttatcg ggaatgactg aaactcaaaa aactggattc ccactttcgt
       61 gggaatgacg ggatgcaggt ttccgtgcgg acgcgttcgg attcacgact gcgtgggaat
      121 gacgggattt cagtttgcgg attgatttga agttgcaaaa tcccaacgga tcggattttc
      181 gctttcgcgt ttcaaagtta cggcgttatc ggaaaaacag aaaaccaaag ctgcgaggat
      241 ttatttaaaa caaccgaatt tcaacgaacc ggattttcgc ctgcgccagg atgacggcgg
      301 aaggtttttt gtcttttctg acagatgtcc gcaatctgac atcccgaccg tgggagcgac
      361 ggttctattt caatccaata taaaatgccg tccgaacgat gttcggacgg catttttaat
      421 gcttcaaatc agttggtgcc gactttgatt ttctgccaca ggctgacaga cagttttttc
      481 gcatccgtgc tcatttgcgg catcacaaaa ccgtctttca tatcctgctc gttcgggaag
      541 atggaacggg tgttcaccag ctcggcaggc attttttcgc gcgccggttt gctggcgggg
      601 gcgaaggtta cggcgatgcc gtttttcgcc gcgatttcgg ggtcgagcgt gtagttgatg
      661 tatttgtggg cattggcgac gtttttcgca tcggcgggaa tcagccaaga ctcaatccag
      721 aagcccatac ctttcggtgt cagcacttcg atgccgacgt tgtttttcac ttcttcggaa
      781 cgtgctttcg ccaagttcaa atcgccgccg ttgcctgccg ccaggcagat gtcgccgcgc
      841 gccagctcgt caatgatgga cgggctgaaa cgtttgacat ccggacggat agacttcaac
      901 acttccgccg ccgccttcaa atcttcagga ttcgatcctt tgggatcttt gcccaagtag
      961 ttcagcaaaa tcgggaacat ttcgctcggg gtgtcccaca gggcgatgcc gcaggatttc
     1021 agtttgtggg tgtattcagg tttgaacagc aaatcccagc cgttttcggg cagcttgccg
     1081 cccaaaagct ctttgccttt cgccgtaatc gcaatcgtgt tcacgccgga gaaatagggg
     1141 acggcatact ggttgcccgg gtcggcggtt tccagcattt tcaagagttc gggatcgatg
     1201 tttttatagt tgggaatcag gtctttgttg actttttgat acgcgcccgc ctcgatttgg
     1261 cgcggcagga aggcgatgcc cggcacaacc aaatcgtaac cggatttgcc ggtcagcatt
     1321 ttggcttcca gcgtttcatt gttttcgtac aagtcgtaag tgagcttcag attgttggct
     1381 tttttaaagt cttcgaccgt actctcgtca acatagttcg accagttgta gatgttcaga
     1441 gtatcggtgg cagcggcttc ggcattggca acagacgcag cgtctgcttg aggttgcacg
     1501 gcgttttttt cgctgccgcc gcaggctgcc agagacagcg cggccaaaac ggctaatacg
     1561 gattttttca tacgggcaga ttcctgatga aagaggttgg aaaaaaaaga aaaccccgcg
     1621 ccccataaac accccggcgc aaggtttggg cattgtaaag taaatttgtg caaactcaaa
     1681 gtgatattgg actgatttcc ctaaaaaatt accctgtttt caaaagaggg ggaaaacgtc
     1741 cgcccgattt tgccgttttt ttgcgctgtc agggtgtccg acgggcggat agagagaaaa
     1801 ggcttgcata taatgtaaac cccctttaaa attgcgcgtt tacagaattt atttttcttc
     1861 taggagattc caatatggca aacagcgcac aagcacgcaa acgtgcccgc cagtccgtca
     1921 aacaacgcgc ccacaacgct agcctgcgta ccgcattccg cactgcagtg aaaaaagtat
     1981 tgaaagcagt cgaagcaggc gataaagctg ccgcacaagc ggtttaccaa gagtccgtca
     2041 aagtcatcga ccgcatcgcc gacaagggcg tgttccacaa aaacaaagcg gcacgccaca
     2101 aaagccgtct gtctgcaaaa gtaaaagcac tggcttgatt tttgcaaaac cgccaaggcg
     2161 gttgatacgc gataagcgga aaaccctgaa gcccgacggt ttcggggttt tctgtattgc
     2221 gggggcaaaa tcccgaaatg gcggaaaggg tgcggttttt atccgaatcc gctataaaat
     2281 gccgtttgaa aaccaatatg ccgacaatgg gggcggagat gaatacacgg aatatgcgct
     2341 atattctttt gacaggactg ttgccgatgg catccgcttt tggagagacc gcgctgcaat
     2401 gcgccgcttt gacggacaat gttacgcgtt tggcgtgtta cgacaggatt tttgcggcac
     2461 agcttccgtc ttcggcaggg caggaagggc aggagtcgaa agccgtactc aatctgacgg
     2521 aaaccgtccg cagcagcctg gataagggcg aggcggtcat tgttgttgaa aaaggcgggg
     2581 atgcgcttcc tgccgacagt gcgggcgaaa ccgccgacat ctatacgcct ttgagcctga
     2641 tgtacgactt ggacaaaaac gatttgcgcg ggctgttggg cgtacgcgaa cacaatccga
     2701 tgtaccttat gccgctctgg tacaacaatt cgcccaacta tgccccgggt tcgccgacgc
     2761 gcggtacgac tgtacaggaa aaattcggac agcagaaacg tgcggaaacc aaattgcagg
     2821 tttcgttcaa aagcaaaatt gccgaagatt tgtttaaaac ccgcgcggat ctgtggttcg
     2881 gctacaccca aagatccgat tggcagattt acaaccaagg caggaaatcc gcgccgttcc
     2941 gcaatacgga ttacaaacct gaaattttcc tgacccagcc tgtgaaggca gatttgccgt
     3001 tcggcggcag gctgcgtatg ctcggtgcgg gttttgtcca ccagtccaac ggacagagcc
     3061 gtcccgaatc gcgttcgtgg aacaggattt acgccatggc aggcatggaa tggggcaaat
     3121 tgacggtgat tccgcgcgtg tgggtgcgtg cgttcgatca gagcggcgat aaaaacgaca
     3181 atcccgatat tgccgactat atggggtatg gcgacgtgaa gctgcagtac cgcctgaacg
     3241 acaggcagaa tgtgtattcc gtattgcgct acaaccccaa aacgggctac ggcgcgattg
     3301 aagccgccta cacgtttccg attaagggca aactcaaagg cgtggtacgc ggattccacg
     3361 gttacggcga gagcctgatc gactacaacc acaagcagaa cggtatcggt atcgggttga
     3421 tgttcaacga cttggacggc atctgaaccg cgtgttcaga cggtatatca agttgccgtg
     3481 ccgtctgaag ccgccggcgg tttggcgggg cgggataaaa atttcgtttg aatccgggca
     3541 aacgcgtata atgtgcggtt tgtacggact ttgtcggaat caagcaagat gacggaacct
     3601 gcggccgaag gcggtaaagc tgccaaggcg ttaaaaaaat atctgattac gggcattttg
     3661 gtctggctgc cgattgcggt aacggtttgg gtggtttcct atatcgtttc cgcgtccgat
     3721 cagctcgtca acctgctgcc gaagcaatgg cggccgcaat atgttttggg gtttaatatc
     3781 ccggggctgg gcgttatcgt tgccattgcc gtattgtttg taaccggatt atttgccgca
     3841 aacgtattgg gccggcagat tcttgccgcg tgggacagct tgttggggcg gattccggtt
     3901 gtgaagtcca tctattcgag tgtgaaaaaa gtatccgaat cgttgctgtc cgacagcagc
     3961 cgttcgttta aaacaccagt actcgtgccg tttccccaat cgggtatttg gacaatcgca
     4021 ttcgtgtccg gtcaggtgtc gaatgcggtt aaggccgcat tgccgcagga tggcgattat
     4081 ctttccgtgt atgttccgac cacgccgaat ccgaccggcg gttactatat tatggtaaag
     4141 aaaagcgatg tgcgcgaact cgatatgagc gtggacgaag cattgaaata tgtgatttcg
     4201 ctgggtatgg tcatccctga cgacctgccc gtcaaaacat tggcaggacc tatgccgtct
     4261 gaaaaggcgg atttgcccga acaacaataa agccgccgtt cagacggcat tttctgtttt
     4321 cagtttaaat caataaaagg tgattttatg cgtaccaact attgcggtct gattagcgag
     4381 caatacttag accaaaccgt taccgtcaaa ggctgggtac accgtcgacg cgaccatggc
     4441 ggtgtgattt ttatcgacct gcgcgaccgc gaaggcatcg tccaagtcgt gatcgatccc
     4501 gacacgcccg aagcgtttgc cgctgccgat tcctcccgca acgaatacgt tttgagcatt
     4561 accggccgcg tacgcaaccg tcccgaaggt acgaccaacg acaaaatgat ttccggcaaa
     4621 atcgaaatcc ttgccaaaga aatcgaagtg ttgaacgctg ccgctacgcc gccgttccaa
     4681 atcgacgacg aaaacatcag cgaaaacgtg cgcctgacca accgcgttat cgacttgcgt
     4741 cgtccggtaa tgcagcgcaa cctgcgcctg cgttaccaag ttgctatggg cgttcgccgc
     4801 tacttggacg cgcaaggttt catcgacatt gaaaccccga tgctgacccg ctccacgccc
     4861 gaaggcgcgc gcgactacct cgtgccgagc cgcgttcatc cgggcgagtt cttcgcgctg
     4921 ccgcaatcgc cgcaattgtt caaacaactg ttgatggtgg cgggtttcga ccgttactac
     4981 caaatcacca agtgcttccg cgacgaagac ttgcgtgccg accgtcagcc tgaattcacg
     5041 caaatcgact tggaaacctc gttcttaaac gaggatgaaa tcatggacat caccgaaggt
     5101 atggcgaaac aagtcttcaa agacgcgctg ggcgtggact tgggcgactt cccacgcatg
     5161 ccttactctg aagccatgtt ctactacggc tctgacaaac cggacatgcg tatcaacttg
     5221 aaattcaccg agttgaccga cctgatgaaa acggaagaat tcaaagtctt ccgtggcgca
     5281 gccgacatga aaggcggccg cgtggttgcc ctgcgtgtgc cgaacggtgc gaaattcagc
     5341 cgcaaagaaa tcgacgaata cactaaattt gtcggcatct acggtgcgaa aggtctggca
     5401 tacatcaaag taaacgatgt gggcaacctt tccaacggcg aagacagcgg cctgcaatcc
     5461 ccgattgtga aattcctgtc cgaaaacgcc ctgaaggaaa tcattgcgcg taccggcgcg
     5521 gaaaacggcg acatcatctt cttcggtgcg gacaaagcca aagtcgtgaa cgaagccatc
     5581 ggcgcattgc gtatcaaagt cggcttggaa cacggcgcgg aaaacggcta tttcacagac
     5641 gaatggaaac ctttgtgggt tgtcgacttc ccaatgtttg aatacgacga agaagccgac
     5701 cgctacgttg ccgtacacca tccgtttacc gcaccaaaag aaggccatga agacctgatg
     5761 gtttccgacc cggcaaattg tttggcgcgc gcctacgata tggtattgaa cggctgggaa
     5821 atcggcggcg gctctatccg tatccaccgc gcagacgtgc aagagaaagt gtttgccgcg
     5881 ctgaaaatca gccctgaaga gcaacaagag aaattcggct tcctcttgga caacctgaaa
     5941 ttcggtgcgc ctcctcacgg cggccttgca ttcggcctcg accgtctggt aacgctgatg
     6001 accggagccg aatccatccg cgacgtgatt gccttcccga aaacacaacg cgcacaatgc
     6061 ctgctgacca acgcgcccaa cagcgtggac gacaagcagt tgcgtgaatt gagcctgcgc
     6121 ctgcgccaaa aggtggctga aaccaaagaa gcataaggaa aacagagccg tttgacggct
     6181 ctgttttttt cagacggcat ttacgcttct tgacttccct ctaattcaaa cctaattttg
     6241 ctgtgtttta atggcggtat tgaaaaacac atcttgttca atcaaccgat aaaaaaagga
     6301 ctgaaaatga aaaaactgtt attggccgcc gttgtttccc tgagtgccgc aaccgcattt
     6361 gccggcgatt ctgccgagcg tcagatttac ggcgatcccc attttgaaca aaaccgcaca
     6421 aaagccgtga aaatgttgga acagcgtggt tatcaggttc acgatgtcga tgccgacgac
     6481 cattggggta agcctgtgct ggaagtggaa gcctataaag acggccgcga atacgacatc
     6541 gtgttgtctt accccgacct gaaaatcatc aaagagcagc tcgatcgctg actcctttga
     6601 tggcaagctg aaccaaaatg ccgtctgaag cgttcagacg gcattttgcc tgttcctcat
     6661 caggtatgag gcaggctttt cttattaaaa aaatgacatt tcacgctgat ttgttataat
     6721 cattcctttt caacacgaca gacggagcag gtttattatg cctatcctta ccatccgtga
     6781 agtgtgcaac attaatcatt ggggcatagg ttattatgat gttgacgatt ccggcgaaat
     6841 catcgtccgc cccaatccgg cgcaacacaa tcaaactgct tcactgcaaa aactgactga
     6901 agccgtgcaa caaaaacatc aggcgcgcct gcctgttttg ttttgttttc cgcaaatcct
     6961 cgaacaccgc ctccgcgaca ttaaccgcgc ctttcagacg gcacgggaag agtgcggcta
     7021 taagggcggt tattgtttgg tttaccctat caaggtcaac caacaccgcc gcgtcatcga
     7081 atcgcttatg tcaagcggac aaccgcatgg tttggaagct ggttctaaag ccgaactgat
     7141 ggcggttttg gcacacgccg gcacccggca aacattaatc gtctgcaacg gctataaaga
     7201 ccgtgaatat atccgtttcg ccttgatggg cgaaaaactg gggcatcagg tttatttggt
     7261 gattgagaag ctgtccgaaa tacaaatggt attggaagag gcggaaaaac tcggcatcaa
     7321 gccccgtttg ggtgtgcgcg ccagactggc ttcccaaggt tcgggaaaat ggcagtcttc
     7381 gggtggggaa aaatcaaaat tcggcttgtc ggcttcccaa gttttgcaac tggtcgatat
     7441 tttgaaacaa aaaaacaggc tggattgcct gcagcttttg catttccatt tgggctcgca
     7501 gcttgggaac atccgtgatg ttgccacagg tgtacacgaa tcggctcggt tttatgttga
     7561 gttgcacaaa ctgggggtaa atatccgctg ttttgatgta ggcggcgggc ttggcgtgga
     7621 ttacgaagga aaccgcacac aatcggattg ttccgttaat tacagcctca acgaatatgc
     7681 cgccacagtc gtatggggca tcagtcaggc ttgtctcgaa cacgggctgc cgcatccgac
     7741 aatcatcacc gagagcgggc gcggcattac cgcacatcac gccgttttgg ttgctaatgt
     7801 tataggcgtt gagcgttaca aaccgcgccg gctggatgcg ccatcgcccg aagcgccgcg
     7861 tgtgttgcac agtatgtggg aaacttggac ggatatttcc gcctcgcggg aaaaacgttc
     7921 cttacgcagc tggatacacg aagggcagtt tgatcttgcc gatgtgcata atcagtataa
     7981 tgtcgggctg ttgagtttgg cgcaacgtgc gtgggcggag caactgtatt taaatatctg
     8041 tcatgaagtc ggcgaattgt ttaatgaaaa acaccggtct caccgaacca ttattgacga
     8101 attgcaagaa cgttttgccg ataagctgta tgtcaatttc tcactcttcc aatctttgcc
     8161 cgatgcttgg ggcatagatc aacttttccc tgtttgtccc attaccggtt tgaatgaacc
     8221 gattgcgcgc cgcgccgtgt tgttggacat tacctgcgat tcagacggta cgattgacca
     8281 ctacatcgac ggcgacggca tcgccggtac gatgcctatg cctgattatc ccgaagaaga
     8341 gccgccgctt ttaggctttt ttatggtggg agcatatcag gaaatactcg gcaatatgca
     8401 caatcttttc ggcgacactg ccactgccga tgttgttgta ggggaagacg gacaatttac
     8461 cgtcatcgat tacgatgaag gaaacaccgt tgccgatatg ctcgaatacg tttatcaaga
     8521 tccgaaagag ctgatgaaac gctatcgcga acaaatcgaa cattcagacc ttcctgcctc
     8581 gcaggctatg tctttcttaa aagaactcga agcggggctt aatggttata cctatttgga
     8641 agacgaatag acgcatcaag gcatcggata tgccgtctga agcccgattt tcttcctcaa
     8701 acaccaatca ccacgaccga ttgaaaccaa ttacaaggaa tcattacgat gcaatacagc
     8761 acactggcag gacaaaccga caactccctc gtttccaata atttcgggtt tttgcgcctg
     8821 ccgcttaatt ttatgccgta tgaaagccaa gcagattggg tcattaccgg cgtgccttat
     8881 gatatggcgg tttcagggcg ttccggcgcg cgtttcggtc ctgaagccat ccggcgcgcc
     8941 tccgtcaacc tcgcttggga gcaccgcagg tttccatgga catttgatgt gcgcgaacgc
     9001 ctgaacatta ttgattgcgg cgacttggtt ttttcttttg gcgacagcag ggattttgtc
     9061 gaaaaaatgg aagcgcacgc cggcaaatta ctttcttccg gcaaacgctg tttgagtttg
     9121 ggcggcgacc atttcattac cctcccgttg ttgcgcgccc acgcccgcta tttcggcaaa
     9181 ctcgcactga ttcattttga cgcgcacacc gacacctacg acaacggcag cgaatacgac
     9241 cacggtacga tgttctatac cgcccccaag gaaggcctca tcgacccgtc ccgttccgta
     9301 caaatcggca tacgcaccga acacagtaaa aaattgcctt ttactgtgtt gtccgcccct
     9361 aaagtcaatg aagacagtgt tgaagagacc gtccgtaaaa tcaaagaaac cgtcggcaat
     9421 atgcccgttt acctgacttt cgacatcgac tgcctggacc cgtcgttcgc ccctgggacc
     9481 ggtacgcccg tatgcggcgg cttgagcagc gacagggcat taaaaatcct acgtgggctg
     9541 acggatctcg acatcgtcgg tatggatgtt gtagaagttg ccccctctta cgaccaatcc
     9601 gacattaccg ctttggccgg cgccacaatt gccttggaaa tgctttacct tcaaggtgcg
     9661 aaaaaggact gaacgtccgg catcccccgg gttttcgccg tgccgttcaa acggcgtatt
     9721 cagtctaatg aaaattcaaa tactgaaaca aaagttgccc ggagccgcat atcggaaaga
     9781 cggtgaaata tcggaatata tcttataaaa caattagtta aatattattt ttccgatttt
     9841 tcgggacggt cttttttacg gaggtcaata tgatgaaatt gggtttcaaa ccgatacccc
     9901 tcgccattgc cgcagtattg tgcgccctgg ttttggcact gcccgtaccc gacggggtca
     9961 agcctcaggc ttggacgctg ctggccatgt ttgtcggtgt gattgccgcc attatcggca
    10021 aggccatgcc gttgggcgcg ctgtcgatta ttgccgtcgg gttggtcgca gtaaccggcg
    10081 taaccgccga caaaccgggc gcggcgatga gcgatgcgtt gagtgcgttc gccaatccgt
    10141 tgatttggct gattgccatc gcagttatga tttcgcgcgg tttgctcaaa acagggctgg
    10201 ggatgcgtat cggatatttg tttatcgccg tttttggaag aaaaacgctg ggcatcggtt
    10261 acagtctcgc tctttccgaa ctgctgctgg ctcccgttac cccttccaat accgcgcgcg
    10321 gcggcggcat tatacatccg attatgcagt cgattgccgg cagttacggc tccaatcccg
    10381 caaaaggcac agaaggcaag atgggtaaat atttggcttt ggtcaactat cattccaatc
    10441 ccatttcgtc ggctatgttt attactgcaa ctgcccccaa ccctttaatc gtcaacttga
    10501 ttgccgaaaa tttaggcagt agtttccgtc tttcttgggg ggcgtgggcg tgggcaatgg
    10561 ctgttcccgg cgttatcgcc tttttcgtta tgcctttgat tttatatttt ttgtatccgc
    10621 ctgaaattaa agaaacgccc aatgccgttc aatttgccaa agaccgtctg agggagatgg
    10681 gtaaaatgtc ggcagacgaa atcattatgg cggtcatttt cggtatcttg ttgctgttgt
    10741 gggcagatgt tcccgccctt attaccggca atcacgcttt tagtatcaac gccaccgcca
    10801 ccgcatttat cggattaagc ctgcttttgc tttccggtgt attgacttgg gacgatgttt
    10861 tgaaagaaaa aagtgcgtgg gatacgatta tttggtttgg cgcattgatt atgatggccg
    10921 catttttaaa taaactcgga ctgattaaat ggttctccgg agtgttggcg gaaagtgtcg
    10981 gcggtttggg cgttagcggc acggctgcgg gcgtaatcct cgtgcttgct tatatgtatg
    11041 cgcattatat gtttgccagt actactgcac atattaccgc tatgttcggc gcatttctcg
    11101 ctgctgccgt ttcactgaat gcccctgcga tgccgaccgc gctgatgatg gcggccgcat
    11161 ccaacattat gatgaccctc actcattatg cgaccggtac ttcgcctgtg attttcggtt
    11221 cgggctacac cacaatggga gaatggtgga aggcgggttt tatcatgagc gtagtcaatt
    11281 ttctgatttt tttcgttatc ggcagcattt ggtggaaagt tctggggtat tggtaaggga
    11341 aaaataaaat aaatttccaa tctgtgttta tttgattggg cgactattat cgtgaaatat
    11401 gccgtctaaa gccttcagat ggcatatttg tgcgcttgaa tgttgcagaa agcggcaggc
    11461 ggcggtgtag gaaaagccaa acaaaaacca aaccgcctat caacttctga taaacataag
    11521 cattaaataa tcagaaggtt attcaattac ctaaacgcaa atttccctta cctaaacgca
    11581 aatttccctg ccgtatcaca tctattgaaa ataatacatc aaccggctcg gaagcagcct
    11641 gatcaggtgt ttctacttgc ggcgatgaat cggcagccgg ttcggtatag gcagtcggcg
    11701 tgccgtcgga ttgggtattt aaatgcggtt gtgcctcggg cgtgtgtaca tcaggcactt
    11761 cgggcgtgcg tgtctcggat atttcggcag agttggtttc ctcagtttgt tcaatgactt
    11821 cagcttggct gtatgaggaa gaaccctgta tccacgccag cgatttgagc ggcatcttca
    11881 tcttgccgtt tttgccgcag gtcaggcaga cggccgatcc ggtgcggtct ttgagtacag
    11941 catcgacttt ctcgggcgcg atggttttac cctgtgtttt tgcccattgc gcaatacttt
    12001 ttttcagaga ggcgatgtca aggttgttct taccgtaagg gtcgcggaag gcggcaagcc
    12061 acaggttgcg atccgcatct tcattgagac ccgccatttg atagatgacg gaggcgggcg
    12121 agccgctggc gatggttttg gcaaactcga gcgcgtcggg cgatttcatg cggacgcagc
    12181 cgtgactccg aaccccgggg acgctggccg gcgcattggt cccgtgtatg cccaaaccga
    12241 gtttggggtc gcctaagcgg acaaaaaccg gccccaaagg gttgtccggg ccggcggcta
    12301 tggtttttac gccgtcgccg cgttctttct gtatggattt ggggatgtac caaacagggt
    12361 tataggcttt cgccccgatt ttatgttcgc ctagattggt ttgcgtcatc gcccgaccta
    12421 ccgcaacggg ataaaccttg gtcagtttgc cgtcggtgta gaggaacagg cgttgctgag
    12481 ggatgttaat gaagacatgt tgaccttgtg cgacggggga gacatcggga atgacggtgt
    12541 ttgcgtatga aaaaccgctt atcaatagtg cagcagtgcg gcagattgtt ttattcatat
    12601 caaaatatgg tgtgtgtccg ataggttttc ggcaaatcat acctgaaacc gtaccaattt
    12661 gtgcgaaaat atgcgcttcg gtacagtgcg gacggattga gtgatggcaa cggaaacaaa
    12721 tgtcgcggaa atttccgcct tggattatga aggcaggggt gtggcaaagg tcggcggcaa
    12781 aacggttttt attaaaaggg cattacttga ttgtttgatg ctgggttggt tcaggcttta
    12841 actcaggaat atttacatca taatgaaggt ttttaaacaa cagcttgaac aactcggcgc
    12901 gcaaaaccaa tatcgttcga ttccggattt gattcatcaa gggcggtata ttacgcggga
    12961 aaactgcaaa atgctgaata tgtcgtctaa tgattatttg ggtttggcat cagatgaaaa
    13021 cttgcgccgg tcttttttgc agcaatacgg cggtaatttt ccctctttta ccagttcttc
    13081 atcgcgttta ttaacgggca actttcctat ttataccgat ttggaagagc ttgtcgcaca
    13141 acgtttccaa cgggaaagcg cgttattgtt caacagcggc tatcacgcca atctcggtat
    13201 tttgcctgct ttgacgacga cgaaaagttt gattttggca gataaatttg ttcacgccag
    13261 tatgattgac ggcatccgtt tgagccggtg tgcgtttttc cgttatcgtc ataatgatta
    13321 tgaacatttg aaaaatctgc ttgaaaaaaa cgtcggaaaa tttgaccgca cttttatcgt
    13381 taccgaatct gttttcagta tggacggtga tgtggcggat ttgaaacagc ttgtccaatt
    13441 aaaaaaacag tttcccaata tttatcttta tgtggatgaa gcccacgcaa tcggtgttta
    13501 tgggcaaaac ggattgggga ttgccgaacg ggataatttg attgccgaga ttgatttatt
    13561 ggtcggcact ttcggtaaag ccttagcctc ggtgggggcg tatgccgtct gcaaccaagt
    13621 attgaaagaa tgtttgatta atcaaatgcg cccattgatt ttttcaaccg cattgccgcc
    13681 gtttaatgtg gcttggactt attttatttt tgaacgattg ccgcaattct caaaagaaag
    13741 aagccatctt gagcagttaa gcgcattttt acggcaggaa gtggcgcatc ggacgcaaat
    13801 aatgccgagc caaacctgta tcgtccccta tattttaggc gggaatgaag ccgcccttgc
    13861 caaagcggaa tacctgcaaa ggcagggtta ttattgcctg cccatcagac cgccgacagt
    13921 acccaaaaac acatccagaa tccgcctgtc tttaacggca gatatgacaa cggatgaagt
    13981 gcggcagttt gcggcgtgcc tgtaaggata tgatatgaaa acaaaatttt acgactgcca
    14041 aggcgaacat ttaattctgt attttgcagg ttggggaacg ccgcccgatg ctgtaaatca
    14101 tttgattttg ccgacaaatc acgatttatt gatttgctat gattaccaag atttacgttt
    14161 ggattttgat ttttcctcct atcggcacat ccgtttggtg gcgtggtcaa tgggcgtttg
    14221 ggcggcagag agggcattgc aaggaataag actaaaatcc gcaacggcag tgaatggcac
    14281 aggtttgcct tgcgatgata atttcggtat cccttgcgcc gtttttaaag gcacattgga
    14341 gaacctcacg gaaaataccc gtttaaaatt tgaacgcaga atgtgtggcg ataaagcatc
    14401 ttttgaagat taccaacaat ttcccgcacg cccgtttgac gaaattcatc aagaacttac
    14461 cgcacttttt gcgatgatcg ggcaagatag gcgtacagat cttatctgct ggacaaatgc
    14521 cttggtcgga tcgggcgata aaatttttat gcctgccaat cagcaccgat attggacacc
    14581 gcgttgcacc gttcgggaaa ttgacggcgg acattacctg ttttcaagat tcacccattg
    14641 gtcggcacta tggaatcact gactgccata aataaatcgc gcattcggca ggctttccaa
    14701 aaagcattaa acgattatga ccggcacgcc ttaatccaac aaaaaatgac gattaattta
    14761 atgacgcatt tgcaagatta tttgccggat atgccattgg aaaacgtgtt ggaattgggc
    14821 tgcggctcag gaatgttgag tgccttgctg caaaaacaga tttcagcgaa ttattggtta
    14881 tttaatgatt tgtgcaatgt gcagccccaa ctggctgaaa aactgccgca atcctttgat
    14941 ttttattgcg gcgatgcgga aaactttcct tttcaacgac aatttgactt aatcgcaagc
    15001 gcatctgccg tgcaatggtt tcatcaaccc gacgctttta tcacccattg caaaacaggc
    15061 ttgaaaacaa acggattatt ggcggttgca acctttggca aagacaattt aaaagaagtc
    15121 cgccaaatta caaatatagg cttaaattac ccgactttat cccaatggca ggcttggtta
    15181 gccaaagatt ttgagctttt atggtgtgag gattttacgg taatactaga ctttgatacg
    15241 ccgtcagatg tactcaaaca ccttaaatat acaggcgtaa cagccacgaa ccaaaaaaat
    15301 tggacaagaa aaaatctcaa tggatttatt ggcgattact tgtcggcgtt cggtatgccg
    15361 tcgggcaaag tgcgcctgac ttatcatccg ttatttttta tcgcgcgcta ctctgcggcg
    15421 ggcaggcaat aaggcagctt atgggcaaag ttatttttat atcgggtatt gatactgatg
    15481 tgggtaaaag gtaatatggc gaggcttgtg cagaaggcat attgttaaac gttaaattat
    15541 ggtatgattt aaaacttaca agtctatttc agtaaatcgt taataataaa agcggacaat
    15601 ggccgttgca ggcggtcagc tatccgctcg ttacggtatc gggagtatcc tttggaaaac
    15661 tcattaatta tcggtttggc actggcggta ttgctgatgc ttttggttat gcgtgcgaag
    15721 cagggtaaga aaacaccgaa gcgcaaatcc caaggtgtcg gcaatacgca ggggcagaca
    15781 cccggaagca atgattcgga ctgggtcgat cagattggac aatcggtttc agacggcacg
    15841 caacccgact ggtcttggaa cgaaagtgcc gagaccgcat ccgccgccgt atccgcgcaa
    15901 gaagtcgatc cgcttacgga gtatcaggtt tataagcaat tcggttatca gggcaaggct
    15961 gccgaatctt tggctgccta tctggacggc attccggatg gtgaagcgaa acctgaaaac
    16021 cttatccgcg agctgctcga tatcaatctc gaagtggggg atgtcgatgt tttggcagac
    16081 aatctgcaaa aatacggcaa actgattcct tccgaacttt tggcaaaata tatcgaacag
    16141 gcattacagc gcgattcaaa ccatttgcgt atccgcgtct tggcggaaga aggtttggga
    16201 tggggtactc aggagattga aaaacgtgcg gaaggcggtt ctgcgacggc agcttccgca
    16261 tcgcccccgc cggatgccgg cggtaaggct tatgaagccg aagaaatcaa gcgcatcccg
    16321 attgtgcggg gcaaaaaaga cgtgtccgga atcagtcaag aggaaatcgg tgcgattgcc
    16381 ggtttggtcc gtgccgatca aggtgcgaaa atccttaaag acaaagtcag ctatgaaacg
    16441 gcatcgaaac aatacgaccg tgccatccaa acttccgaaa aacctgcaaa cctgattatc
    16501 gatgcgttga aactcgatta ccaatacgcg gacatagacc gttttgccgg acatttgtgg
    16561 aaactttacc aaacgttggg caactacggc aggcaggtta aagagcggat gctggggtgg
    16621 gggtacagcc tgggttacca tgaggttttc gatgatttgg aaaaagggcc gaacgaccgg
    16681 caaatcaaag acatcggtat ggggcacggg tatctgccga aaaatataca gaaattcaaa
    16741 tcgcaacatc gggatttggt gcttcaagat tcttcgttga tcaacaccgg ttcgtctccg
    16801 gcagacgatg cggttaagga agtagagtcg ttgctgatgt atggtcagat tgaagcggca
    16861 atggatgtgt tggagcaggc ggtattgaaa tatcccgacg agtcccagct ttatattacg
    16921 ttgatcgata tttatgaacg tactgaagat tgggataggt tggggcagtt tttaagggta
    16981 ttgagggaac gtgcggacag gcttcctgaa gaggtcgtta tgctgatgag ccggctgctg
    17041 cagcgtatga atcaaaatat taaaaaaata aaacggtacg gaaaataaaa atggaagttc
    17101 aactgccgaa aattaaaaca gtacgcgtaa tgttggcggg gatgacggcg cagcaggaat
    17161 ccgttttcaa aatggcattc aaaatgcaca ataccacccg ttatgaaaca gtatcccctt
    17221 cagacggcag tgccgtgccc gatttggttt tggcggatac cgatgccgag ggcggttttg
    17281 aactttggaa agagcttgcc gagcgttata aggatatacc cgtcgccgtc tgttcggaga
    17341 aagttcccga ttctgaagtt ccctacctgc ccaaaccgat tcggtttgaa acattgtttc
    17401 ctatgctccg caaattgttg cagggcgaga atgtttatgg gaaatcgttt attgcacccg
    17461 cagaccggtc ggcgaaaaat aacgggaatg tgcagcgtac ggttacgata cgccagttta
    17521 acccgaataa aggattattg ggggcgttgc ggtttgcgga aaagaacagg caggacatcg
    17581 ctatcttgca tggaaataag ccggtcctta ttgttttccc ctcgatacaa cgggttttgc
    17641 tgacagaaag tgtgcaaaaa ctcgaagaat tgtgcaaaga cgaaaatttg caggtcagct
    17701 gcaagactgt tcccgataac ccgcaatggc gcgaaaaggc taaagtaggc attatgtcct
    17761 gtatgtggca gttttccatt tggacggcgc agggcaggtt gatttatccg atttctcccg
    17821 atactccgtt tacgttgaaa tcttggccaa acctgacccg gttggcaaat gtgccggggt
    17881 cgatacgctt gtcggcattt ctgaccaagg catccgtcaa ccttaacgtg ttgtataaag
    17941 tgatgccttt aaacctcaat gatattctga attatcttgc ggcaacctat acaaccgggt
    18001 ttttgtcggt agatttaaaa acggtttcac aacaggcata ctccgatatg gcggataaaa
    18061 taaatatcgg agccgattct gcctctgata gtgaaatgat gaaaaaagcg gaaaaaatca
    18121 caacaccatc ccaatcccag tcgcgcggtc ttctgcaaag gctgatgaaa aaactgttgg
    18181 gcagctaaga ggcggagaga tgagagaaaa taaaattatt ttcacaggac ctgtcggcgt
    18241 agggaaaacc actgccattg cggctatttc ggacgaagca ctcgttcaga ccgatgcttc
    18301 cgcatccgat atgactttgg ataggaaaag gaatacgaca gtggcgatgg actacggggc
    18361 catcagcttg gatgaggata ccaaagtcca tttatatggt acgcccggtc aggaacggtt
    18421 caactttatg tgggaaatct taagccaagg cagtatgggt ttggtcttgc ttttagataa
    18481 tgcccgaacc aatccgttga aagatttgga attcttttta cattcgtttc gagggctgct
    18541 ggagaaggca cccgtcgttg tcggtattac caagatggat atacgctctc agcccggtat
    18601 cgacgtgtat cacaaatatc ttgcaaaaca taatcttaat gttccggttt ttgaaattga
    18661 tgcccgtaag gaagatgacg taaaacaatt ggttagcgca atgttatttt ctattgatcc
    18721 gggactggag gtttaatatg gaatcaacac tttcactaca agcaaattta tatccccgcc
    18781 tgactcctgc cggtgcattt tatgccgtat ccagcgatgc ccccagtgcc ggtaaaactt
    18841 tgttgcacag cctgttgaaa gcggatgcgg acgaaatggt cagcagtgag aagctgctta
    18901 cttgggcgga caccgccgac atcgataccg ctttgaacct gttgtaccgt ttgcaaaaac
    18961 tcgaattcct ctatggcgat gaaaacggtc attcagacgg catcaatttg tcggacgagc
    19021 aattgccgtt gctgatggaa caattgtccg gcagcggtaa ggcgttattg gtcgatcgga
    19081 acggtctgta tcttgccaac gccaatttcc atcatgaggc ggcggaagag ttggggttgt
    19141 tggcggcaga agtcgcacag atggaaaaga aataccggct gctgattaag aacaacctgt
    19201 atatcaacaa taacgcttgg ggcgtttgcg atccttccgg tcagagcgaa ttgacatttt
    19261 tcccattgta tatcggttca accaaattta ttttggttat cggcggcatt cccgatttgg
    19321 gcaaagaggc atttgttact ttggtaagga ttttataccg ccgttacagc aaccgcgtgt
    19381 aaaacttggg agagaggagg ggttatgcag caattattga tttcaatcct tgaagattta
    19441 aacaatacat ctacggatat tatcgcgtct gccgttatct caaccgacgg attgccgatg
    19501 gcgacaatgc ttccttcaca tttgaattcg gacagggtag gggcgatttc tgccactttg
    19561 cttgctttgg ggagtcgctc ggtgcaggaa ctcgcctgcg gggaattgga acaagtgatg
    19621 attaaaggaa aatcaggcta tatcctttta agtcaggcgg gtaaagatgc cgtgttggtg
    19681 ctggtggcaa aagaaaccgg cagacttggt ttaatcctat tggatgccaa acgtgcggca
    19741 aggcatattg cggaagccat ataacatata aagattgcgg gcttgcagat aaagtgcaat
    19801 cgattgtcaa tttatattga cacgttcggt atttctgttt tattattcgc gcttattccc
    19861 cgatagctca gtcggtagag cgacggactg ttaatccgca ggtccctggt tcgagcccag
    19921 gtcggggagc caaatttcaa aaccctctaa gtattttctt agagggtttt gttttaccgg
    19981 cggtcagaaa cgcatttttg agatgattgt tttgagatgg aataaaatct ttgcaaaatt
    20041 cctttcgtga tggttatgaa aaaatagggg ctgtcctgga caactaggat aaactcgatt
    20101 ttatagtgga ttaacaaaaa ccagtacggc attggctcgc cttagctcaa agagaacgat
    20161 tctctaaggt gctgaagcac caagtgaatc ggtttcgtac tatttgtact gtctgcggct
    20221 tcgtcgtctt gtcctgattt ttgttaatcc actatactaa ttgagacctt tgcaaaattc
    20281 ctttccctcc cgacagccga aatcccaaca caggttttcg gctgttttcg ccccaaacac
    20341 ctcctaattc tacccaaata cccccttaat cctccccgga tacccgataa tcaggcatcc
    20401 gggttacctt ttaggcggca acaggcgcac ttagcctgag acctttgcaa atttgtcggt
    20461 ttcggggtcg tattggtagc ctcgtgcctg tatgtcttct ttgaaagttt cgtatacgtc
    20521 atgggctaaa agggctgttc cgacataggg aaccgccctt gtgctgaatt tcgcgcctaa
    20581 gcgggcaagt ttgccgcccc ccgccaatac gccggcgcgg gaaatgctgg cggttatttt
    20641 ggcgttgatt cgggcttttg cgcccgtagg gatgtgtgtt aaatctaccg tttttattaa
    20701 atcagatgaa taagttttac tatttttagg tacaaactta tgaattttcg caccttgtcc
    20761 ggtatcaact gaaacagttt cagatatttt tactgcattt gcattcgctt caaacgaata
    20821 catcatcaaa attgcaatta tcgacaattt cgcaaaattc aaatttgtat attttatgac
    20881 catctttcag ggattcttta attaccattt ctgaattatc agaaaatgag attagccaaa
    20941 tatcatgttt aattcttcta ttccagaaaa aagagaaaca atcaataaca ttttcagact
    21001 tattaatctt cgcaaattca acaaattcag attgcgctat aaccgccatc gattgcccaa
    21061 aatacttgct agacggctga tatttataaa gtgccaactg cgcctgagtg ataaacggct
    21121 tgttcatggt tctgcctttc aatgattgtt ttgaaagcct gattttgaca ccataacttc
    21181 atgcgctcaa ttcttaaaca gaaccgcccc gattaatacg ggtacggaaa cgccgagata
    21241 aaaataaaaa tccatcattt caaaaccttt ttcagcaggg aaacaaagta aacggacgcc
    21301 atcacgccga atactatcca gcctgtttca aggccgcttt gcaggttgtc tttcagactg
    21361 cattccgcca ataaaagcct tagcggctga ccgtccgaca tcttccacag gctgccgtta
    21421 tattccggcc tgacaatctg tccgttttct ttgattcttg gtactaccaa gctgaaataa
    21481 aggttttcag cctggtgctt ctcaagacat ttatttccga cttggtagta catgccgtct
    21541 tacttcatca ctctcttaac gatggaaaag acaaaaagcg cggcgaaaat gcccactaca
    21601 atccaaccga cttccatacc gtcggctttt gcggcttcca aagcgttttt tgctgtatcg
    21661 ggcaacgttg catttgcatg tgcggccaaa gccaggggag cagctgttac aacagccagt
    21721 tttgcgccgt atttacggca ggtgttaata aatttcatga tattttcctt caaaaagtgt
    21781 ttggcggtaa tggatggagc gtttttcaga cgaccgccga acatccgaaa atcagtcttt
    21841 caaaaatccg aatacgacaa attcgtattg gttgccgatt tcttccaaac ctgcgttaat
    21901 cgcttcttcg aagtcgtaga aataatcggc attggtgatt aatttggtat gtccgatgtc
    21961 gcccgtttca ggagagagat acagaaagtc ccctgttgat acggactgga caacatagac
    22021 tttctgcatt caatcagcct ttcttcacga gttgaaaacc gatgactttc agtttttggg
    22081 ttttgcccgt agtaacgatt tccacgttca ggtttgcttc gatgggaaat tgggcgtttc
    22141 ggaactgctc gaaattggca gagccgccga aatcgtattc agtagtagag ctgcccaatg
    22201 cgttgccttg ggagctgtct aagggtgtgg cgacaatcag gcggcaatag tcgaagctct
    22261 tgccttcgat ttgtccgttg aattttttaa cgccgacgat gtggccttga agttggatgt
    22321 tcattttttg gtttccttgt gtgattaaac gtctttcggg cagacacttt aagcccatga
    22381 aatcggtagt cttgcgaatt tgtcgtaaat gaagttgtta tagctttctt cattgttgac
    22441 gtgtttttgc tgttcaagct gtttttcaag attctcgtaa tattcgtaca tatagtaagg
    22501 gtctttgtac ggtttgaatg cgggctgttc atgaatggct tgagctttca aaaaggcgca
    22561 gtcgtaggct tcgggagtca aagacttggg cagcttgtga tgactcggct caatcagttc
    22621 aaacagtttg gctttgtcca attcgggaaa aatgaatttc agaccgtttg ccgcacgtcc
    22681 gaactgtttt ttacccattc aaggtaacgg tcggctgaaa tgaccttatc ttccttaacc
    22741 gcgtgtatgc gcgttgcctt ttggacgaat cgttcgcaaa tcggatatgc gccgccgaaa
    22801 tattcgcccg gattctgcaa aacttcaaaa gggataacga tgtcttttgc tttgaattca
    22861 atttcaaagc gcgtccatgt gcttgtttta tcgcccaact gcttgccttt ttcatagacg
    22921 cggacatatt tggacgattc acgggagccg ataccatagg tcttgccttt ggtcattttg
    22981 gcttcatcgt cttcttccca atctgacccc aaacattcgc cttttggttt gacgtgatga
    23041 caggtaaaca tacctttatt tcggtcttca cgggcttggt tcgggctgta ttcgccgttg
    23101 aaaaagtctt ttgcgatgtc aacgcgtgtg atttttgggc ggattgcatt agtcaggaat
    23161 gcgaaaagtc gtgattccca gccttctttt gcgacgccgc aaccggtgcc ggtcagttcg
    23221 aaaagaatgg tattgttttg tccgccaaaa tggacgcgac cgtatagggc gtcttccgaa
    23281 cccatcaacc aacagcgctc ataaaaacgc ccgcccgagc ctttggattc tttgtagata
    23341 ccgaaaccga aaacttcttc ggcgagcatt gacgcggcgc gaataaaatc ttcgtcttcc
    23401 aaaagactta cacgaacgcc gtatttatcg aaaaaggg
//
LOCUS       DAWFLT010000024        22247 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWFLT010000024 DAWFLT010000000
VERSION     DAWFLT010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22247
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.024"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(120..1034)
                     /locus_tag="WGR74_000984"
     CDS             complement(120..1034)
                     /locus_tag="WGR74_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0905422.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WGR74_000985"
     CDS             1290..2759
                     /locus_tag="WGR74_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ0905423.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="WGR74_000986"
     CDS             3011..3382
                     /locus_tag="WGR74_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905424.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVE
                     SQCRAELNKRSEWRLTALAMSAEKQAEWENKICACVAQEAPNQLTGNDVMQMLDPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /locus_tag="WGR74_000987"
     CDS             3455..4864
                     /locus_tag="WGR74_000987"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ0905425.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWTEVAAGKGFDLQFALDKVGGKYGFTSSKSTHADRLATIKQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDAAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4910..5704
                     /locus_tag="WGR74_000988"
     CDS             4910..5704
                     /locus_tag="WGR74_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905426.1"
                     /translation="MTLPMQETRFSILLDELAAKQKAAIAPHLLADGSKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRAAGIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKSDAWLAGLEAIARVHKKRQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5892..6668
                     /locus_tag="WGR74_000989"
     CDS             5892..6668
                     /locus_tag="WGR74_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0905427.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHSRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(7326..9797)
                     /locus_tag="WGR74_000990"
     CDS             complement(7326..9797)
                     /locus_tag="WGR74_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ0905428.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRAGMDTPIAVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGNPAVRRIELLGSMTEAQVLAVAQSLEQQSEHPLARAILNCRISGGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVWQLAGKNLTLHILSGDRETAVAETARALGITHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMPSEKMPSEQ"
     gene            complement(9794..10972)
                     /gene="hflX"
                     /locus_tag="WGR74_000991"
     CDS             complement(9794..10972)
                     /gene="hflX"
                     /locus_tag="WGR74_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ0905429.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSATRLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKILQCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLREIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(10977..12257)
                     /locus_tag="WGR74_000992"
     CDS             complement(10977..12257)
                     /locus_tag="WGR74_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ0905430.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHASYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGSGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGDIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            complement(12410..13129)
                     /locus_tag="WGR74_000993"
     CDS             complement(12410..13129)
                     /locus_tag="WGR74_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ0905431.1"
                     /translation="MDTTRIVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAMLAS
                     GIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAKGM
                     EFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGRRAAAP
                     YDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPIKL
                     GVLYYGDESTQYEPAPIRVDK"
     gene            complement(13200..13877)
                     /gene="radC"
                     /locus_tag="WGR74_000994"
     CDS             complement(13200..13877)
                     /gene="radC"
                     /locus_tag="WGR74_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ0905432.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSRSVCSF
                     RQLGLMP"
     gene            complement(14000..15349)
                     /gene="gshA"
                     /locus_tag="WGR74_000995"
     CDS             complement(14000..15349)
                     /gene="gshA"
                     /locus_tag="WGR74_000995"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ0905433.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15609..17018
                     /gene="leuC"
                     /locus_tag="WGR74_000996"
     CDS             15609..17018
                     /gene="leuC"
                     /locus_tag="WGR74_000996"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ0905434.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17115..17369
                     /locus_tag="WGR74_000997"
     CDS             17115..17369
                     /locus_tag="WGR74_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905435.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17431..18072
                     /gene="leuD"
                     /locus_tag="WGR74_000998"
     CDS             17431..18072
                     /gene="leuD"
                     /locus_tag="WGR74_000998"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ0905436.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPSGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18241..19512
                     /locus_tag="WGR74_000999"
     CDS             18241..19512
                     /locus_tag="WGR74_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0905437.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLETLGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPNLQNILDSLTDKGYLVLEHPKQKIGGRRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDKYDLLGNTVAVPVIKAVSERLLHTL"
     gene            19528..20259
                     /locus_tag="WGR74_001000"
     CDS             19528..20259
                     /locus_tag="WGR74_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ0905438.1"
                     /translation="MVKLTAQQIFDKLLNEEKILSVNGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNAGPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            20293..21363
                     /gene="leuB"
                     /locus_tag="WGR74_001001"
     CDS             20293..21363
                     /gene="leuB"
                     /locus_tag="WGR74_001001"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ0905439.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDISYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAILAAL"
     gene            21526..22089
                     /locus_tag="WGR74_001002"
     CDS             21526..22089
                     /locus_tag="WGR74_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ0905440.1"
                     /translation="MKKIIFAALAAAAISTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPIANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMLKTEVCGGDFSTTIDRT
                     KWGMDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5355 a         5857 c         5787 g         5248 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcagaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta tcaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc ggcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcggggacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttgtggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ccttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccggg ggcggtaaca cattcggcag cttagacggc ggcacaggta tgggcggcag
     3121 catcgtcaaa atggcggtag aaagccaatg ccgtgcggaa ttgaacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgccga aaaacaggcg gaatgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacccaacca gctgaccggc aacgatgtga tgcagatgct
     3301 ggatccgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatgc cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcccccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgcgaaacc ttggacaaat ggcgcggttt
     3601 ggcttatccc gaattggcgt ttgaaattat gcgcctgttt gttacggata ttccggagga
     3661 cgatttgcgc gacattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttgtcca acggcccgac
     3781 gctggcgttc aaagatatgg cgatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggc aaaaaactca atatcttggg cgcaaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaaaggtgt gaatgtattt atgctgtcgc ccgatggcaa
     3961 aatgagcgcg ttccaacgtg cgcagatgta cagcctgcaa gacgggaata tccacaatat
     4021 cgccgtgaag ggtatgtttg acgactgtca ggatattgtg aaggcggtgc aaaacgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac ggtcaattcg attaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgccggcta tttcaaagcg acgcaaagca atgacgagca
     4201 agtcagcttt tgcgtgccga gcggcaactt cggcaacgtt tgcgcgggac atattgccaa
     4261 acagatgggc ttgcccatcc gccgcctgat tgtcgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggcg cgtaccgccc gcgcaacagc gcgcatactt atgttacctc
     4381 cagcccatct atggacattt ccaaagcgtc caatttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctcaggaaa tcaatacgct gtggacggaa gtcgcggcgg gcaagggctt
     4501 tgacttgcag tttgccttag acaaagtcgg cggcaaatac ggctttacct ccagcaagtc
     4561 cacccacgcc gaccgcctcg ccaccatcaa acaggtttac gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgcgaag aaggggaaac
     4681 ggtggtttgt ttggaaaccg cgttggcagc gaaattcgat gcgaccatac gcgaagccgt
     4741 cggcgatgcc gccattccgc gccctgccgc gctggaaggt ttggaaaacc tgccgcagcg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatgttcc gtacaggggc actccgtccg aacttaagat tgaaagaaaa tgaccttacc
     4921 tatgcaggaa acccgttttt ccatactgct agacgaattg gcggcaaaac agaaggcagc
     4981 catcgccccc cacctgcttg ccgacgggag caaggtatgg atacgcaaag caggcaggca
     5041 caatgcgcga tggcgttacg cgctgctcgg tatggttgcc cgatatttga aactgggggt
     5101 gttgaagccg gtccccagcc tcggcggcga gcctgccatt gcaaccgaat caaaacgcct
     5161 gtacgaattg cgcgcggcgg ggatagccgt tcccgaattg ctcgccgtcc ggaaaaatgc
     5221 ccttatgttc ggcaatttgg aaggcatccc gctcgatacg caaatccgcc aagaagccga
     5281 ggctggaaag tcggacgctt ggcttgccgg tttggaagcc attgcacgcg tgcataaaaa
     5341 acggcagttc ctcagtcagg cgtttgcgcg aaatatgatg tcggacggaa aaaacatcag
     5401 ctttttggat tttgaagacg acccttccga agtcctgacc atagcgcaat gccaagcccg
     5461 cgattggctc tgctacatcc attcgaccgc gctgatattg aaaaacggcg gactgctgga
     5521 agcggctgcg gaaaaatggg gcggcgtatt gtcagatcag cctgccgaaa tacaaaagct
     5581 gattgccgcc accgtcaaac cgatcctccc gatacgcagg ctggaacacc cccgctgggg
     5641 acgggacgcg ctcaggctgg cagcctcgat ttccctgatt tcccttgccg atatgccgcc
     5701 gtaagccgcc gcccgcccca cgatgccgtc tgaagccctt cagacggcat ataaccaacg
     5761 cgcatcaagc attgtcgcaa ccatccgttt cgtttagaat tttggcttta ccccgaatcg
     5821 ggcagacatt ttcactctgc cgcacatccc gaacaagcac tatccatttc ttttactgac
     5881 aggaaaccca aatggcagca ttcaataccc aaaaagtatt gtccgtacac cactggacag
     5941 acgcatattt tacctttacc tgcacccgcg acgaatcgtt gcgctttgaa aacggacagt
     6001 tcgttatggt cggattgatg gtggacggca agccgctgat gcgtgcatac agcgtcgcct
     6061 ccgccaactg ggaagaacac ctcgaatttt tcagcattaa agtccaagac ggcccgctga
     6121 ccagccgcct gcaacacctc aaagtcggcg acgacgtgtt aatcagcaaa aaaccgaccg
     6181 gaactctagt tgccggcgac ctgaatccag gcaaacacct ttacttgttg agcaccggta
     6241 ccggcatcgc ccctttcttg agcatcacca aagaccccga gatttacgag caatttgaaa
     6301 aaatcatcct cgtacacggc gtgcgctaca aaaaagattt ggcgtactac gaccgcttta
     6361 ccaaagaatt gcccgaacac gaatacctcg gcgacttggt taaagaaaaa ctgatttact
     6421 acccgattgt ttcccgagaa gaattcgaac accacagccg cctaaccgac ctgatggtga
     6481 gcggcaaact gtttgaagac atcggcctgc ccaaaatcaa tcctcaagac gaccgcgcga
     6541 tgctgtgcgg cagccccgcg atgctgaaag acacctgcaa agttttggac gatttcggac
     6601 taaccgtttc tccgaaaaca ggcgtgcgcg gcgattacct gattgagcgc gcgtttgtgg
     6661 atcaataagc tgcttgaaaa tgccgtctga acggcatcag cgataaaaaa acccgaatcc
     6721 tgccaatcag gattcggggt ttttatagtg ggtaaaaatt agaaatgcac atttccgtca
     6781 ttccaacaca ggtgggaatc cggacttacc tgtttcatta tcgtttgaaa ataaaagaga
     6841 aaccaaacat atagattccc gcttgcgcgg gaatgacgac ggtgttttgc cgtagccaaa
     6901 gcagaaaaac atccctattg tcatttcatc atgatatgac aataggatgt taaaaattaa
     6961 ttatcattat atgattgata tataagaaaa actgtcccaa taagaaatgt tatgcagaaa
     7021 ataaaatgaa aaggatggtt ttcattgaaa atggttgttc tgccaccgtc atacacagaa
     7081 ccggtatttg gaaaaaaaat aatccatatt gacatcatta ttccatttat cgcataaatc
     7141 cactttttaa ttttttttcc tcattaatat aatctcttat ttaataaaat caaataccta
     7201 gcaattcgat ctcaaatttt aaacgccaac gtgtttgagc ggtaacaatg catactttaa
     7261 gtttgtaatc cttcatcgtc tcatttttgt tttaatcgac tatacgtcta aagcaacgcc
     7321 gtccgtcatt gttcggacgg cattttttca gacggcattt tcccccgttt gtgaaggcgc
     7381 agggcgttgc ccaaaaccgc cagcgaactg aagctcatac ccagtgcggc tatccacggt
     7441 tggacatagc ccaaaacggc aagcggtacg gcaatgatat tgtacgcgcc cgcccatatc
     7501 aggttttgcc ggataatatg gcgggtgcgc cgcgcctgat cgagcaggtg ggcgacggta
     7561 cgcaaatctt cgttcaataa cacaatgtcc gcgccgtccc tcgcaatatc cgtcccgccc
     7621 gctgcggcgg cggatacgtc tgcctgcgcc aaaacgggcg cgtcgttgat gccgtcgcct
     7681 atcatcagca cttttttccc ttctttttgc aaggctttga cgtattccag tttgtcctcg
     7741 ggcatggctt gggcgcggta gtgcgtgata cccagggcgc gggcggtttc ggcaacggcg
     7801 gtttcgcggt cgccgctgag gatgtgcagg gtcaggtttt tgcctgccaa ctgccacacc
     7861 gcctccgccg cgctgtcttt caaggggtcg gtcaggtaga acacggcttg gaaaccgctt
     7921 tgactgccga ggtaaaccgc gctgccgccg ccttctgttt gcggttcttt acctgaaatt
     7981 tcggcgacat aggatgccct gcccaatgcc caaacctgtg tttcgccgtt gacggtcagt
     8041 tgcgcgccca cgccttcgcc gatgcggttg aggcgttgtt taatagcaat gtcggggacg
     8101 ctgccgcctg aaatgcggca gttgaggatg gcgcgcgcga gcgggtgttc ggactgctgt
     8161 tccaaacttt gcgccactgc cagcacttgg gcttcggtca tgctgccgag cagttcaatc
     8221 cggcggacgg cgggattgcc ttgggtcagc gtgccggttt tgtcgaaaat gatgtcggtg
     8281 gtttgggcga gggtttcgat tgcctgcttt ccgccgatta aaataccttc gcgcgccagc
     8341 gtaccggtag aagctgccag cgcggtcggc gtggcaagcg ataaggcgca ggggcaggta
     8401 atgaccagca gggcgacggt aatccacaat gcggtgtgcg catcggcgta caacgtccag
     8461 ccgatgaaga ccgggacggc aagcagtagt tcgccgaata tgaaagacga ggcgtattgt
     8521 tccgccaact cggcggtgcg cggtttttgc gccaaggcgc ggtcgagcaa gcggacgatg
     8581 tgcgacagtc gcgtgccgcc gccggtgcgg tcggtgcgta taatcagggg gctttgcgtg
     8641 ttgagtgtgc cggcggttac tttttcagac ggcattttgg cgacgggcag gctctcgccg
     8701 gtcagcatag attcgttgac ggcactgctt ccttccagca ccgtgccgtc aacggggatg
     8761 gtttcgcccg gtttgaccag cacgatatcg cccgccttca atttgacgac agctgcctcg
     8821 caggtttcct gcgtatcggg gtaatcgggc atatgatggc aaaacgcagg aatcagcttc
     8881 accagccttt cggcagcatc gcctgcctta cggcgggcaa tgtgttccat aaagcgtccg
     8941 cccagcagga aaaacagcag catggctatg gattcgaaat acatcccctg ccccgcattg
     9001 gtcgcaaggc tgtaaacgcc ggcgataaag gtcatgatga tggcgacggc aatcggcgta
     9061 tccatgccgg cgcggcggtt tttcaagtcg cgcagcgcgc cttgataaaa cgggacggcg
     9121 caatagaata cgacgggcag caccattaaa aagccgcccc aatggaggat ttgcaggaaa
     9181 tcgggttcga tgtcgccgcc gtaaaggtag gtcggcagcg cgaacatcat cgtctgcatc
     9241 atccccagcc cggcaacggc gaggcggacg atgtattgtt tgcgttcttt ttggttggcg
     9301 gcttcgattt tttgcgcgtc atagggtgcg gcggtgtagc ctatctgcct gattttcaac
     9361 agaatgtcgg aaaggcggat tttgccgtcg tcccagacga cgcggcagcg gtgcgtgctg
     9421 taattgaggt cgatgcggac gatgccgtct gtacgcaaaa gctgctgttc gatcagccag
     9481 acgcaggcgg cgcaggtgat gccgccgagc attaaaaccg cctcgcgcgt gccgccgtgg
     9541 gtttccacaa agtcggactg gacttcgggc aggtcgtaca ggcggatttg gtcgaggatt
     9601 tcttggggcg gcagctcggt tttttgcgcg tcggcggtgc gttgtttgta ataactgccc
     9661 aagcccgcgt caataatgct ttgtgcgacc gcctgacagc cggcgcagca ggtttcgcgg
     9721 tcttcgtttt cgtaacggac agtcaggtgg aggtgttcgg gaacatccag cccgcagtgg
     9781 aaacaggttt ttttcatggc atttcggttt cgtctgtgtt tggtgcggcg gcacaagact
     9841 cggcaatggc ttcgcgcagt gcgtctatac cggtattttc agcaacggaa atgcggacgg
     9901 cggcaatttt tcccgcagcg tcgcgccata tgcccgtgtt ttgttcttca gacggcagca
     9961 ggtcggtttt gttgtacacc ttgatgcacg gaatatcgcc ggcatggatt tctcgcagta
    10021 cgttttccac gtcttcaatc tgctgtccgc tgttcggagc ggcggcatcg acgacgtgca
    10081 gcagcacatc ggcttgcgcg gtttcttcca gcgtggcgga aaaggcggaa atcagtttgt
    10141 gcggcagatc gctgacgaat ccgacggtat cggtcaggat gatgctgcat tcgggactga
    10201 tgtacagccg ccgcgccgtg gtgtcgagtg tggcgaaaag ctggtctttc gcatatatgc
    10261 ccgacttggt cagccggttg aacaggctgg atttgccgac attggtatag ccgaccagcg
    10321 caaacgtttt gattgtgccc gattcgcggg acttgcggcg cagggcgcgc tgttttttga
    10381 ggttggcaag ctgttttttc aaggcgttga ttcgatgggc gatcaatcgg cggtcggttt
    10441 ccagtttggt ttcgcccggc cctttcatgc cgataccgcc gcgctggctc tgcagatggc
    10501 cgtaaccgcg tatcaagcgt cccgccaaat ggctcaattg cgccaactcg acttgcagcc
    10561 tgccttcctg cgtgcgggcg cggcgggcga aaatcgccaa aatcaaccct accctgtcca
    10621 atacgcggca ttggaggatt ttttccaaat tgcgttcctg cgtgggcgta agttcgtggt
    10681 tgaatacgac caaatcgatg ccgtctgcgg caactgcttc cgacagctcc gccgccttgc
    10741 ccgtgccgac aaacagcgcg gtgtgcggac ggtcgcgttt ggcagtctcc acgcgcacgg
    10801 aatcgccgcc cgccgctttg accagctcga cagcttccgc caatgccgtc tgaaaaccgt
    10861 tcagacgggt ggcactgctg cccgtgccat ctttgtccaa cattacgccc accagcatga
    10921 cgcgttcggg ttgcgcttgg gtactgccgt tgcggcctgt tctgcctgtc atgtattcaa
    10981 tcctgtaaat aaagggttac gcctcgttcc gtttctcctt ccggtgcaag cgtgatgcgg
    11041 ctgcccgcca ttccgtcaac gctgaagacg atgtcgcccg cctcgtgcct tcctatggac
    11101 aggcggctgc cgggtcgctg ccggtaggta taacgggtac acgcttccaa acaccagcgt
    11161 ccgcgccagt ggatgaaata tttttcgcct tttctaaccg caagtgtctc gccgccgttt
    11221 acctcaaaat aagggtaatc gggttttcgg gttctaaact gccatttcgc ctgcgctcgc
    11281 cgtccgttgc ggacatagtc gaataccgcc gtataaagcg tgccgtattc caaaggcttg
    11341 agcggaaaaa gcgcgaattg gtacgcggtc agcctgccgt tggggtcgtt gccggcggtt
    11401 aaaaccctga cggggcggat ttcgttttta ccctgataca gcttgaaact tttcatcgta
    11461 attttgcctg ccgcctcgga aaaatcaatg ctggcaggat tgcccgtgat ttcatattcc
    11521 ggcacggggt ctggacgctc gccgtggaaa taaggcagtg cgccgctgcc gacgggatag
    11581 gctgtataga gcagctcctg tgcgggcatg gcttcgtcgg tgtacacgac cgcaccgtta
    11641 tggcaggcgt tgcggtaata tttccgtcct gcttccggct gatttctgcc ttgggcgcaa
    11701 tgccgctcaa acctgccgtt gccctgattg aatacgagaa cggttttacc gttttcgcgc
    11761 acaaatgccg ctcctgcctc atccgtatgg cggtcaagta gggaaaggcg gtggtagatt
    11821 gcgctcatta atccgtccac ttggcgttgc tgcgtgcgga tgtcgctgtc ggacgattcg
    11881 gcggcttctt cttccgtgct gatgttttca tgcacgccgt tgtagagata cccggcaagg
    11941 cgtgtgcgtt cggtcagctt ttgtgcggtg tagtgcggat tgtcgggatg gtgttcgccg
    12001 tgtccgtctt cgggattgag cgtgaggtag cttgcgtgcc tgcgggcgga gttttccaaa
    12061 accggcgcgt gtgccagctt gtgcaaaccg atttgggctc ggatgcggtt gaggtagtgc
    12121 agcgcatcgc cgccgtccct gatttgcggt gcggacggat agacaagttc gcccgcgggc
    12181 agggattggt tttgggtatg gtagaaaacg cctgccgccg aacccaacag gagcagccga
    12241 ataaaaaggg atttcataat gccgtcctcc cgacgcggca acggggtgtt ttatcaaaac
    12301 ggcaaacttg ccaatgcggt ttccgattgc agattgccaa accggcaaac ctttccccgt
    12361 gccgatgccg aacccgatgc cgtctgaacc ttcagacggc atcgggtgtt tatttgtcca
    12421 cccggatggg ggcaggttcg tattgtgtcg attcgtcgcc gtaatacagc acgccgagtt
    12481 tgatgggaat gcgtccctgc gatttgcggt gggcattgga atcgcgcaag gaataggcgc
    12541 aaccgcaata ttcctgctgg tagaagtttt cacgtttgct gatttcaatc atgcgcgcgc
    12601 tgccgccgcc tttgcgccag ttgaaatccc aatacaccac atcatcgtaa ggcgcggcgg
    12661 cacggcggcc gcagtcgttg atttgcgcca tatttttcca gcgtgaaatg cccagcgaac
    12721 tggtaaagac ggggaacccg tgttcatgcg cgtattgcgc cgccttttca aaacgcatat
    12781 cgaaacacat ggtgcagcgg atgccgcgtt ccggctcaaa ctccatgcct ttggctttgg
    12841 caaaccattc tttgcggtcg ttttcgtagt cgtcgtcttt atcgatgaaa ggaatgccga
    12901 acttttccgc aaagcgcacg ttttcctctt ttcggagcat atactctttg tgcggatgga
    12961 tattgggatt gtaaaaataa atggtgtagt cgatgccgga ggcaagcatc gcctccatca
    13021 cttcgccgct gcacggggcg caacaggaat gcagcaagac ttttttatgt ccgccgggcg
    13081 ggacgagtat ggggcggtcg atgtcggtaa cgatgcgtgt tgtgtccata gtttcaaata
    13141 gatgttctgt ttggtgcaaa cggctatttt atcagagccg ccgcatgtga aacagagtgt
    13201 cagggcatca acccgagctg cctgaacgag cagacgctcc gggaggttac gataaaatgg
    13261 tcgagcagcg acacatcgac cagcgacatg gcctgtgcca gccgccttgt gaacatgatg
    13321 tcttcctgcg aaggttcggg cgagccgccc ggatggttgt gcgcaataat caggctgtcg
    13381 gcatattcgt ccaatgccag tttgacgatt tcgcggatgt aaatcgtgtt ttccgcaacc
    13441 gtaccgcgcg acagctctct gaccgcaatc agttggtttt ggcggttcag cagcagcgcg
    13501 acgctgactt cgactttttc ctgccccaaa tgaaagcgta aataatcggc aacggtatcc
    13561 ggatcggaca ggacgatgct ctcctgcaat tcttccgcca atatccgccg cccgatttcc
    13621 ctgactacgg caaactgtgt gaaacttgcc gtccccatcc ctttgtatgc cgacagtttg
    13681 ccgacctccg cgctcatcag cctccccaaa ctgccgaact cctgcagcaa ataacgcgcc
    13741 aaatcgaccg cactcattcc gcgcgtgccg acgcgcagca ggattgccaa aagttcggca
    13801 tcactcaaag ccgccgcccc gcgttccaac agcttttccc tgggtctttc gccttccggc
    13861 cattgcttga tgctcatgtt ttcccttttt taaatagtct gagatgccgt ctgaaacaaa
    13921 tgttcccaca cgccaaatat ccgaaatgcc gtctgaaccg gatttccgct tcagacggca
    13981 tctgtatttc aaaacagtct tacgccgttt cttccaactc gattgaagcc gccagaagcg
    14041 acaggcgtgc cattacgccg taaacgtaga ggcggttgtg ttcgttgtcc acgtcgcagt
    14101 ctttttcaga gcgcggcata cccagcgagt cccattgttc gaatacgcgc ttgcaggctt
    14161 cgggcgcttc ttgggagttg tcgccgttgc cggtggggat gctgttggac agcggaacaa
    14221 acaccatacc gccggcgttg aggttttcgt ccgcaccgcg cccttcgtgt acgcggaaaa
    14281 agccgccgat gacgaagcgg tccatcatat acacgacggg ttcgcacacc gcgccgttta
    14341 aggtttcata agtataaatc ccttcttgga caatcacttc gctgacttcc aaaccttctt
    14401 tgactttcgc cattttattg cggtttttac ggttcaatcc gcgcacttcg tcggcggatt
    14461 tgacgctcat cacgcccatg ccgtaagtgc cggcatcggc tttgacgatg acgaaaggtt
    14521 tgtcggtaat gcccgattcg tcgtatttgg cttgaatttt cgccagcaca cgttctaccg
    14581 cttccgccaa cgcgtcttcg ccttcacgcc cttggaagtc caaaccgccg attttttcaa
    14641 aataagggtt aatttgccat tcgtcgatgc cgattaactt ggcaaattcg gcggtaactt
    14701 ggttgtacgc gccgaaatga tttgttttgc ggcgcgtcgt ccaaccgccg tgcaacggcg
    14761 gcaaaacggt ttggctgatg cctttgagga tgtcgggaat gccggcagac aaatcgttgt
    14821 tcaacaaaac cacgcaaggc gaaaagccgt ctgcaagatg gacgcggtcg cgggtacgca
    14881 ataaaggttc caacaggatt ttgtcgccca atgcagtttc aaattcggtc ggttcggtta
    14941 cttccggatt caggctgccc aagcgcactt cataccctgc cgaacgcaaa atctcgccga
    15001 gggcgtaaat gttttgcagg taaaacgtat tgcgcgtgtg gttttcggga ataatcaata
    15061 cggatttcgc cgtttcgcag gcgcgttgca ccgcatcttg cgcggcaacc gccgccagcg
    15121 ggataaagtt gggattcaga ttattgaagc cgccggggaa caaattcata tcgatagacg
    15181 aaattttgta accggcattg cgtatatcga ccgaaccgta aaacggcggg cggtgtacgc
    15241 tccactgcgt gcggaaccac gcctcgattt tggcgtgatt ggacaggatt tttgcctcaa
    15301 acgcctgaag ttgcgccgaa tgttcgggcg acatcaccgg taatttcatc tttcgtctgc
    15361 ctctgtgtgt tttttgacaa gatgcagatg ataatgcctt tgaaagaatg acgacaagta
    15421 ttctttcgta tcgaatcatg ctttagatat aactgtttac tgaaatggac aatacgtcta
    15481 attttttcgt taaaattgtt tgcaaaataa caccggctca aaaattagac aaaatttgtt
    15541 gcgcggtata aagaatacgt ctaaaatccg cccaatccgc attaccattt atccaaaaga
    15601 acaacatcat gacagcacaa accctctacg acaaactttg gaacagccac gtcgtccgcg
    15661 aagaagaaga cggcaccgtc ctgctctaca tcgaccgcca tttggtgcac gaagttacca
    15721 gccctcaggc atttgaaggc ttgaaaatgg cggggcgcaa gctgtggcgc atcgacagcg
    15781 tcgtctccac cgccgaccac aacaccccga ccggcgattg ggacaaaggc atccaagacc
    15841 cgatttccaa gctgcaagtc gatactttgg acaaaaacat taaagagttt ggcgcactcg
    15901 cctattttcc gtttatggac aaaggtcagg gcatcgtaca cgttatgggc cccgaacaag
    15961 gcgcgaccct gcccggtatg accgtcgtct gcggcgactc gcacacttcc acccacggcg
    16021 cattcggcgc actggcgcac ggcatcggca cttccgaagt cgagcacacg atggcgaccc
    16081 agtgcattac cgcgaaaaaa tccaaatcca tgctgattgc cgttgacggc aaattaaaag
    16141 cgggcgttac cgccaaagac gtggcacttt acatcatcgg gcaaatcggc acggcgggcg
    16201 gtacaggcta cgccgtcgag tttggcggcg aagccatccg cagcctttct atggaaggcc
    16261 gcatgacttt atgcaatatg gcgatcgaag ccggtgcgcg ctcaggcatg gttgccgtcg
    16321 accaaaccac catcgactat gtaaaagata aacccttcgc acccgaaggc gaagcgtggg
    16381 acaaagccgt cgagtactgg cgcacgctgg tgtctgacga aggtgcggta ttcgacaaag
    16441 aataccgttt caacgccgaa gacatcgaac cgcaagtcac ttggggtacc tcgcctgaaa
    16501 tggttttaga catcagcagc aaagtgccga atcctgccga agaaaccgat ccggtcaaac
    16561 gcagcggtat ggaacgcgcc cttgaataca tgggcttgga agccggtacg ccattaaacg
    16621 aaatccccgt cgacatcgta ttcatcggct cttgcaccaa cagccgcatc gaagacttgc
    16681 gcgaagccgc cgccatcgcc aaagaccgca aaaaagccgc caacgtacag cgcgtgttaa
    16741 tcgtccccgg ctccggtttg gttaaagaac aagccgaaaa agaaggcttg gacaaaattt
    16801 tcattgaagc cggttttgaa tggcgcgaac cgggctgttc aatgtgtctc gccatgaacg
    16861 ccgaccgcct cgcaccgggg caacgctgcg cctccacctc caaccgcaat tttgaaggcc
    16921 gtcaaggcaa cggcggacgt acccacctcg tcagccctgc catggcagcc gcagccgcag
    16981 tgaccggtcg ctttaccgac atccgcatga tggcgtaagt attttcaggc ggtctttaag
    17041 aatttgacgc ggccgtctga aatatgtccc gtaaggcaga aacaccttta atattcaaac
    17101 caaggagttt aaccatgaac aaacttttca ttaccgccct gtccgccctc gccttgtccg
    17161 cctgcgccgg cacttgggag ggcgcgaaac aagacaccgc ccgcaacctt gacaaaacac
    17221 aggccgccgc cgaacgcgcc gccgaacaaa caggcaacgc cgtcgaaaaa ggctgggaca
    17281 aaaccaaaga agccgtcaaa aaaggcggca atgccgtcgg acgcggcatt tcccatctcg
    17341 gcggaaaaat cgaaaacgcc accgaataac cgattcgcga tgccgtctga aaccgcttca
    17401 gacggcatcg tcagcacaag acgcaaaccc atgaaagcct ttaccaaaat taccgccatc
    17461 gtcgccccgc tcgaccgcag caacgtcgat accgacgcca tcatccccaa acaattttta
    17521 aaatccatca aacgcagcgg cttcggcccc aatgcctttg acgaatggcg ttacctcgac
    17581 cacggcgaac cgggcatgga caacagcaaa cgcccgttga accctgattt ctcgctgaac
    17641 cagccgcgtt accaaggcgc gcaaatcctg ttgacgcgta aaaacttcgg ttgcggctct
    17701 tcacgcgaac acgccccttg ggcattggac gactacggct tccgcgccgt catcgccccc
    17761 agcttcgccg acatcttctt taacaactgc tacaaaaacg gccttttacc tatcgtgttg
    17821 accgaagaac aagtcgatcg gcttttcaaa gaagttgaag ccaacgaagg ctatcagctc
    17881 tccatcgacc ttgccgaaca aaccctgacc acaccaagcg gcgaaacatt cacattcgac
    17941 attaccgaac accgcaaaca ctgcctctta aacggcttgg atgaaatcgg tctgaccctg
    18001 caacacgccg acgaaattca cgccttcgaa gaaaaacgcc gccaaagcca gccttggctg
    18061 tttaacggtt aagcctatgc cgtctgaaga ccgttcagac ggcattcctg ataacatata
    18121 attgctgcaa ctaatgtaaa cagaggggct ataaattgct ttcatctttc aagcgggctt
    18181 agcaacgctc taaattaatg caatacagct ctatttcaaa taaattttta gatttttaat
    18241 atgcaacaga ttaaatttat tgacttattt tccgggatga gcggtatcag gaaaggattt
    18301 gaacaagcct gtcggaaaca gtcggttgct tgcgaatgtg tttttacctc agaaatcaaa
    18361 ccggcagctt tggaagtatt gaagcaaaac taccctgatg aagtgccgta tggagatata
    18421 acgaaaattg aaacgggaga tattcccgat tttgacatct tgttggcagg cttcccttgc
    18481 caggcttttt ctttcgccgg aaaaagattg ggctttgaag atacgcgggg aacgcttttc
    18541 tttgatgtgg caaggatttt aaaggcaaaa aaaccaaaag gttttatttt ggaaaatgtg
    18601 gaaggattgg tgacgcacga tagaaaagat ccgacacaaa aaataggacg caccctgacc
    18661 gttattttgg aaaccttgga aacattgggc tactatgttt cttggaaagt tttaaatgca
    18721 aaagatttcg gcattcccca aaacaggaag cgcatttatc tgacaggcag tttgaaatcc
    18781 aaacccgact tgtcttttga aacaacccta agtccgaaat taaaaaatat tttggaatcg
    18841 ggactgccta ccgaaagcag tcctttcatc aaaaaattgc taaagaaatt tcccccgtcc
    18901 gaactgtacg gaaaatcagt aaaggacaaa cggggaggga aaaacaatat ccacagttgg
    18961 gatattgaat taaaaggcac agtaaccgaa gaagagaagc aattattaaa tatgcttcta
    19021 aaagaacgga ggaagaaaaa atgggcttca gaaatcggca tcgattggat ggatgggatg
    19081 cctttgacaa aagcgcaaat ttcaactttc tataaacacc ccaatcttca aaatattttg
    19141 gacagcctga cagataaagg ctatttggtt ttggagcatc cgaaacaaaa aattggcggg
    19201 cgaagaatca aagatgaatc cctgcccaaa ggctacaata ttgtttcagg aaaaaaatca
    19261 tttgaaatca ataaaatatt agacccaaat gatgttgcgc caaccttagt tgcaatggat
    19321 atggagcacc ttttcgtcgt tgacaacggc ggtttgcgca cacttaccgg aaaagaaggt
    19381 ttacgcttat tcggctatcc ggacgattat tcgtttgata ttcctaaaaa agacaaatac
    19441 gatttattgg gcaataccgt tgccgttcct gtgattaagg cggtatctga aaggcttctg
    19501 catactttat aaaccgatag ggataaaatg gtaaaactta ctgcacaaca aatatttgat
    19561 aagttgctga atgaggaaaa aatcttatcg gtcaatggtc aaattagatt tttcttgggc
    19621 gatgtggata ttatcgtcaa acaaaaagat gtcgtcggca acatcattca agaatggctc
    19681 ggcggatggt tgagaaaaag ggaaattgaa tttgatgttt caaccaatac ccaaatgccc
    19741 cccgatttct ttttaaataa aaaagaccgc agtagagaat tgttggaagt aaaggcgttc
    19801 aaccgaaatg ccggcccggg ttttgatatt gcagatttta aaatgtattc tgatgaaatc
    19861 attcataaac cctatatgct ggatgtagat tatttaatat tcggttacga tatggacgac
    19921 aacggcaatg taaccatcaa ggatttatgg cttaaaaaag tatggcaaat tacgagaagc
    19981 atggatgggt gggcaattaa tcttcaagtc aaaaaaggcg tggtgcataa aatccgcccg
    20041 ggtgtttggt acagcataaa taaaaagaat atgcctatgt ttgaatgctt ggaagatttt
    20101 gtttccgcaa ttgaagaaac cgtttatcaa aacccggcaa cacggcataa cgcatcctta
    20161 tggaaaaaga aatttgagga agcctataaa aaacactaca accgatcgat ttccatacct
    20221 cgttggcacg aaattgcaca caaatacaaa aagaaataac ccttcaacca tcaacccata
    20281 aggaaacttg aaatgaccaa acatatcgcc atcctccgag gtgacggcat cggtcccgaa
    20341 atcgtcgccg aaaccgtccg cgtactcgac aaactcatcg cccaaggctt ggacatcagc
    20401 tacgaatacg ccccattggg tggtgaagcc tacgacgaat acggccatcc ttatcccgaa
    20461 tttacgcaaa acctctgccg caaagccgat gccgtcctgc ttggtgcagt cggttctcct
    20521 caatacgaca acctcgaccg tccgttgcgc cctgagcgcg gcctgttggc aatccgtaag
    20581 gatttgaacc tgtttgccaa tttgcgtcct gctgttttat atcccgaatt agccaacgcc
    20641 tccacgctga agcctgaaat cgttgccggt ttggatattt tgattgtgcg cgaactcacc
    20701 ggcgacatct atttcggcga accgcgcggc atccgcgttc tggaaaacgg cgaacgcgaa
    20761 ggctacaaca ccatgaaata cagcgaaagc gaaatccgcc gtattgccca tgttgccttc
    20821 caatccgccc aaaaacgcag caaaaaagtc tgttccgtag gcaaagccaa cgttttggaa
    20881 accaccgaac tgtggcgcga aatctttgaa gaaatcggca aacaatatcc cgatgtcgaa
    20941 ctctcccata tgtatgtgga caacgccgct atgcagctcg tgcgcgcgcc caaacaattt
    21001 gacgtgattg ccaccggcaa catcttcggc gatattttat cggacgaggc ctctatgctg
    21061 accggctcca tcggtatgct gccttctgct tctttggacg aaaacggcaa aggcctgtac
    21121 gaaccgtctc acggctccgc tcccgacatc gccggacaaa acaaagccaa cccgctggcc
    21181 accatcctct cgctcgcaat gttgctgcgt tacagcctga acgacgaagc gcgcgcgcaa
    21241 caagttgaaa acgccgtcca aaaagtgctg caacaaggct tgcgtaccag cgacatttac
    21301 gaagaaggca caaaactcgt ttcctgctcc gaaatgggcg atgcgatact cgccgccttg
    21361 taaaaaaaca aagcgcggat cacaccgcgc tttttcatct gcccttttcc gcccgcaccg
    21421 ttcgtcaacc gcctgcaaac ccaccgttta ccggcaggaa atgccataaa tccctgccta
    21481 tgttaggata aacgccttta ccggcccaca caaaggatta tcatcatgaa aaaaatcatc
    21541 ttcgccgcac tcgcagccgc cgccatcagt actgcctccg ccgccaccta caaagtggac
    21601 gaatatcacg ccaacgcccg tttcgccatc gaccatttca acaccagcac caacgtcggc
    21661 ggtttttacg gtctgaccgg ttccgtcgag ttcgaccaag caaaacgcga cggtaaaatc
    21721 gacatcacca tccccattgc caacctgcaa agcggttcgc aacactttac cgaccacctg
    21781 aaatcagccg acatcttcga tgccgcccaa tatccggaca tccgctttgt ttccaccaaa
    21841 ttcaacttca acggcaaaaa actggtttcc gttgacggca acctgaccat gcacggcaaa
    21901 accgcccccg tcaaactcaa agccgaaaaa ttcaactgct accaaagccc gatgttgaaa
    21961 accgaagttt gcggcggcga cttcagcacc accatcgacc gcaccaaatg gggcatggac
    22021 tacctcgtta acgttggtat gaccaaaagc gtccgcatcg acatccaaat cgaggcagcc
    22081 aaacaataaa tcttgatgcg aatcagcaca aatgccgtct gaacgtcctt caaacggcat
    22141 ttgtgccgtc ttcatcaaac cgccccgcca acggtaagcc gatatggcgg agcacaatcc
    22201 gccgaaagat atttcagcgg caggcagggc ttgccggaaa gtccgga
//
LOCUS       DAWFLT010000025        21905 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWFLT010000025 DAWFLT010000000
VERSION     DAWFLT010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21905
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.025"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(131..1087)
                     /gene="gshB"
                     /locus_tag="WGR74_001003"
     CDS             complement(131..1087)
                     /gene="gshB"
                     /locus_tag="WGR74_001003"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ0905441.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
     gene            complement(1180..2868)
                     /locus_tag="WGR74_001004"
     CDS             complement(1180..2868)
                     /locus_tag="WGR74_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ0905442.1"
                     /translation="MLNKDQFADNHFIRTIIEDDLKSGKHEAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLIKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSVEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIHAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFEAGRTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTIERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            complement(2980..3753)
                     /locus_tag="WGR74_001005"
     CDS             complement(2980..3753)
                     /locus_tag="WGR74_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ0905443.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EDAGSLKRHHGGASSGGNLPEGLPASRQIQCQNEKNAIARLIAEHIPDESSLFVSIGT
                     TMEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            complement(3796..4698)
                     /locus_tag="WGR74_001006"
     CDS             complement(3796..4698)
                     /locus_tag="WGR74_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ0905444.1"
                     /translation="MDSLMTLLSVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFASGKLMRDIWMPSENAGMYCLMLLVLLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASADGVSWVKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIALLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIRGAGGLEAVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            complement(4847..5563)
                     /locus_tag="WGR74_001007"
     CDS             complement(4847..5563)
                     /locus_tag="WGR74_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ0905445.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            complement(6132..6554)
                     /locus_tag="WGR74_001008"
     CDS             complement(6132..6554)
                     /locus_tag="WGR74_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ0905446.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(6672..7343)
                     /locus_tag="WGR74_001009"
     CDS             complement(6672..7343)
                     /locus_tag="WGR74_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ0905447.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDAVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QAAKP"
     gene            complement(7359..7985)
                     /locus_tag="WGR74_001010"
     CDS             complement(7359..7985)
                     /locus_tag="WGR74_001010"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ0905448.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AGQGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSVLPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(8092..8910)
                     /locus_tag="WGR74_001011"
     CDS             complement(8092..8910)
                     /locus_tag="WGR74_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0905449.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(9169..9609)
                     /locus_tag="WGR74_001012"
     CDS             complement(9169..9609)
                     /locus_tag="WGR74_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ0905450.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSEPSVLLACGGNLPRIPEGMAVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEELADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(9672..11078)
                     /locus_tag="WGR74_001013"
     CDS             complement(9672..11078)
                     /locus_tag="WGR74_001013"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ0905451.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDVHAFEEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFAEAVFHSAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLVDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            11248..12363
                     /gene="lptF"
                     /locus_tag="WGR74_001014"
     CDS             11248..12363
                     /gene="lptF"
                     /locus_tag="WGR74_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ0905452.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLDLRNGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            12360..13430
                     /gene="lptG"
                     /locus_tag="WGR74_001015"
     CDS             12360..13430
                     /gene="lptG"
                     /locus_tag="WGR74_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ0905453.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGLIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGIWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            13740..16325
                     /gene="acnB"
                     /locus_tag="WGR74_001016"
     CDS             13740..16325
                     /gene="acnB"
                     /locus_tag="WGR74_001016"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ0905454.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFTSRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            16467..17462
                     /locus_tag="WGR74_001017"
     CDS             16467..17462
                     /locus_tag="WGR74_001017"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ0905455.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQETVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPASIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(17531..17632)
                     /locus_tag="WGR74_001018"
                     /pseudo
     CDS             complement(17531..17632)
                     /locus_tag="WGR74_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(17686..18699)
                     /gene="ilvC"
                     /locus_tag="WGR74_001019"
     CDS             complement(17686..18699)
                     /gene="ilvC"
                     /locus_tag="WGR74_001019"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ0905456.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(18779..19072)
                     /locus_tag="WGR74_001020"
     CDS             complement(18779..19072)
                     /locus_tag="WGR74_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ0905457.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAA"
     gene            complement(19139..19630)
                     /gene="ilvN"
                     /locus_tag="WGR74_001021"
     CDS             complement(19139..19630)
                     /gene="ilvN"
                     /locus_tag="WGR74_001021"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ0905458.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            complement(19641..21368)
                     /gene="ilvB"
                     /locus_tag="WGR74_001022"
     CDS             complement(19641..21368)
                     /gene="ilvB"
                     /locus_tag="WGR74_001022"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ0905459.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADGKA"
BASE COUNT         5062 a         5978 c         5657 g         5208 t
ORIGIN      
        1 ataccgtctg aaaacggggc ggcggggtgt ccgtacggca ttaaccgcat ccctgccggc
       61 tgagggaaaa ccctgcctgc ccgaacaaac caaacggttg tgaagcaaaa gcctttcaaa
      121 cggcatcggt ttaacgtacc gaccacgcgg caacggcatc ggcaaacatt gccgccacgt
      181 cgaaaccttt ttgtttcata atttcttgga atccggtcgg gctggttacg ttgacttcgg
      241 tcaggttgct gccgataacg tccaaaccgg ccagcaggat gccgcgccgt ttgagttcgg
      301 gggcgagcgt ttcggcaatt tcgcggtcgc gtccgtccaa ttcctgcgcc acgccgcgtc
      361 cgcctgccgc aaggttgccg cgtgtttcgc cgttttgcgg gatacgcgcc aaagcatagg
      421 ggacgacttc gccgccgata atcaggatgc gtttgtcacc gtgtacgatt tcgggaatgt
      481 agcgttgcgc cataatggtg cgggaatcaa gccgcatcag ggtttcgagg atgctgccga
      541 tgttggggtc tttttcggtc aggcggaaaa tacccatacc gcccataccg tccagcggtt
      601 tgatgataat gtcgccgtgt tctttcaaaa atgcgcggac atcggcggaa cgggtcgtta
      661 ccagcgtggg cgcggtaaag cggctgaagt tcaaaatcgc cagtttttca ttgaagtcac
      721 gcatcgactg tccgctgtta aagactttcg cgccctgctg ttccgccagc gtcagtaatt
      781 gggtggcgta gaggtattgc atatcgaacg gcggatcggt acgcataatc acggcatcaa
      841 atgctttcaa tgccgtctga actttgtcgg cggcggcaaa ccagtttttg tcgtggtcgt
      901 cttgtgcgcc gagaaactca aaagcagacg catcagcaac caccagaccg tttttaaccg
      961 acaattcccc gctcaaggta tgaaacagcc gccaaccgcg ttttgccatc tcgcgcatca
     1021 tggcgtaggt ggtgtctttg taggttttga aacttgccat cgggtcggcg ataaagagga
     1081 ctttcatcat atttcctttc cggtgtgccg aatgtgccgc atttcgcggg caaaggggaa
     1141 atgccgtccg aacaatattc agacggcaag gatggggttt tacttaggct gccaagaatc
     1201 tttcagcgtg accgtgcggt taaacaccgg cgtgtctttg ccgtggtctt tacggtcggt
     1261 tacgaagtag ccgatacgct cgaactgcca acggctttct gccggcaaat ctttggcggc
     1321 aggttcggcg taggcggtga tttccttgac ggattccgga ttgaggaaat cggtgaacgg
     1381 caggtattcg ccgtcttcgc cgcgcacggc atcgggacgc tcgatggtaa agaggcggtc
     1441 gtacagacgg actttgattt cggcggcgtg ttcggcggaa acccaatgaa tcacgccttt
     1501 aactttacgg ccttctggat ttttgcccaa ggtgtcgtgg tcgatgctgc atttgagttc
     1561 aaccacattg cctgcttcgt ctttgacgac ttcatcgcac ttgatgacat agccgtggcg
     1621 caagcgtact tcgccgccgg gaatcaggcg tttgaagcct ttgggcggat tttcggcaaa
     1681 gtcgtcggct tcaatataga tggtttgtga aataggtact tcgcgctcgc ccatttcctc
     1741 gtggttcgga tggaacgcgg cacggcggct ttgggttctg ccggcttcaa agttggtcag
     1801 ggtcactttg agcgggttca acaccgccat caggcgtggg gcggaatttt ccaactcttc
     1861 gcgaatcgcg ccttccaaca cgctcatatc gacgatgttt tcagatttgg aaataccggc
     1921 gcgtttggca aacagacgca ggccttcggg cgtgtagccg cgtcggcgca taccggaaat
     1981 agtcggcata cgcggatcgt cccagccgga aacgtgtttt tccacaacca actgattcaa
     2041 tttccgtttg gaggtaatgg tgtacaaaag ctccaaacgg gaaaactcgt attggcgcgg
     2101 acgggtggca tgcggcgcgt ggatgttatc caacacccag tcgtacagcg gacggtgtgc
     2161 ttcaaattcg agcgtacaca aggaatgcgt gatgccttcg atggcatcgg agatgcaatg
     2221 cgtgtagtcg tacatcgggt agatacacca tttgtcgccg gtgttgtggt gatgggcgcg
     2281 gcggatgcgg tagatgacgg ggtcgcgcat attgatgttg cccgatgcca tgtcgatttt
     2341 caggcgcagg gttttgctgc cgtcggggaa ctcgccgttt ttcatgcgtg tgaacaggtc
     2401 gaggttttct tcgacgctgc ggtcgcggta agggctgttt ttacccgctt cggtcagcgt
     2461 accgcggtat tcgcgcattt cttcgggcgt caaatcatcg acatacgctt tgccgtcttt
     2521 aatcaaaccg acggcgtagt cataaagctg gtcgaaatag ttggaagcga aacgcggctc
     2581 gcccgcccaa tggaaaccga gccactcgac atcttctttg atggcgttga cgtattcgtc
     2641 gttttctttt tcggggttgg tatcgtcaaa acgcaggttg cacaagccgt cgtaaatata
     2701 cgccaaaccg aagttcaggc agatggattt ggcgtgtccg atgtgcaggt agccgttggg
     2761 ctccggcgga aaacgggttt ggacggcttc gtgtttgccg cttttgaggt cgtcttcgat
     2821 gatggtgcgg ataaaatggt tgtccgcaaa ttggtcttta ttgagcatag ttttctttga
     2881 acagatggct tcagacggca ttggggcggt tccttatgcc gtctgaagcg gtttgatgat
     2941 gtgtttattg tacccgactt gcgcgctttg acatagcgtt cagacggcat cggcaatcag
     3001 gcattccacc cccgcctctt tcagcatctt ctgcatcgcg gtatcgggca gccggtcggt
     3061 aaatactttg tcaaacgccg taatgtcgcc gagcctgacc agcgcgttgc tgcggaattt
     3121 actgtggtcc acgccgagga agcggacgcg cgcgttggca atcatcgcct gcatcacgct
     3181 gacttctttg tagtcgtcgt ccaaaagcga accgtcgctt tccacgccgt gcgtactcat
     3241 cacggcataa tcgactttga actggttgat aaaatcgacg gttgccacgc cggtaatgcc
     3301 gccgtccaaa gggcggacga ctccggaagt gatgatgacc gtgtaatccg tccgcgccga
     3361 aacgatggag gcgacgtgga tattgttggt aataatccgc aggctgccgc gccgtctcac
     3421 cagctccgat gccacggctt ccatcgtcgt gccgatactg acaaacagcg atgactcgtc
     3481 gggaatatgt tccgcaatca gccgggcaat ggcgtttttt tcgttttgac attgaatttg
     3541 gcggctggca ggcagcccct ccggcaagtt tccgcccgag gatgcgccgc cgtggtggcg
     3601 tttcaggctg ccagcatcct ccaattcgcg gatgtcgcgg cgtatcgtct gcggggtaac
     3661 gtccaatgcg gcggcaagct cgtccaccga cataaactga tgccggcgga caaggcttaa
     3721 aatctctccg tgcctttgga ttttcggttt catcgttttc tgcctccttg catcgggatg
     3781 ccgattttac cgcgttcagc ccaaagcgga aaacaccacc atcagaaacg gagcggcgat
     3841 attgaccacc acgccgaagc tgaccgctac cggtacggct tccaagccgc ccgcaccccg
     3901 aatcacgggc aatgtgaaat ccatactggt cgcgccgccg acccccactg ccgcatcggg
     3961 aaaacgcttc atcagcagcg gaataaatgc cagcgcgaac agctctcgtg ccaaatcgtt
     4021 caaaagcgcg atactgcccc ataccgcgcc gtaagcctcg gtcatcacca aacccgagag
     4081 ggaataccaa ccgaagccgg aagccatcgc caaacctttc acccacgaca caccgtctgc
     4141 cgatgcggca aacagcagcc cgcctgaaag agatgaaagc ataaaccaga ccgacaaccg
     4201 aatacccctg cggttgacca aaacctgccg caacgatacg ccgctgcttt tgagctgtac
     4261 gccgatgagg agcaccagca gcatcagaca atacataccc gcgttttcag acggcatcca
     4321 aatatcgcgc atcagtttgc cggatgcaaa tccgagcagc acgcatccga gctgccccac
     4381 actgcccgac acaccgaccg aaacgccctt ccctttcccc tttatccgcc acgggaataa
     4441 ctttcccaac actgccaaag caagcaggtt cgccccgacc gtacaaacaa acagccacag
     4501 aaccgtcaac gccatatcgt ccaaccgcga acccaaatcc tccacgcgcg acaacgagac
     4561 gccgatcagc agcagcacag catacaccaa gaccgatagc accttatcca aagcgggcag
     4621 gtaaggcttg ggcacacgga taaaaaatcc ggcaaacatc ggtatcaata ccgaaagcaa
     4681 cgtcatcagg ctgtccatct cctgacctcc tttattgccg catgatatat gcggtttaaa
     4741 aattgccgtc tgaaaattgc agatacccgc atccatattt cagacggcat caggttcgcc
     4801 attaaaaaac cgcctgaagg ttcaggcggc ttatccgctc cggcattcaa tcttccaaag
     4861 tcttttccaa acgctccata cagttgccca aatggcggcg caggattttg accacgcggt
     4921 tgcgcctgcc cgccagcagc aggtcgagga tttcgcggtg ttcggaatgc gtatgcgtat
     4981 tgatggcgtg tttttcctcg cgatgcacgc ccgccacggc gacaatcagg gaagaccgcg
     5041 cgcacagcgt gttcataatg tcgaacagca catcgttgcc caccaggcgc gccagttcga
     5101 cgtgaaaggc attggacagg cggttccagc caacgcggtc tcccctgccg gaggcctctt
     5161 cttcgcgccg tatcatcgca taaagcggct tgaggcgcgt ttccaaatcc ggcaaatctg
     5221 cgaggatatt caaaatcatc gtctccattt cgatgcgcgc attgaacaca tcctgcattt
     5281 ctttcaaatc gggaacgtgg acgaacgcgc ccctgttggg ttgcaaatcg acaatcttgt
     5341 cgtgcgccaa aagcgacagc gcgccgcgga cggtgttgcg cgaacacacc atctggcggc
     5401 aaagttcgga ttcggtcagc tttttgccag gcagcagcac ctgatcggta atgccgtcca
     5461 aaatcagggc gtaaacacgg aacagctccg aatcgtgccg ctcttcgaga atcagggaag
     5521 acgtggtcgg cgcatggata atgtcgtcgt tttcaaagtt catgatgttt tccgtatttt
     5581 tacgctttca aatttcttaa gatgttttaa ggcggctgtg tttcaaatcg tgtcggggaa
     5641 attaaagcat tgcacaaatt tattttatag tggattaaca aaaatcagga caaggcgacg
     5701 aagccgcaga cagtacaaat agtacggaac cgattcactt ggtgcttcag caccttagag
     5761 aatcgttctc tttgagccaa ggcgaggcaa cgccgtactg gtttttgtta atccactata
     5821 attcaataaa ttaatatatg gcttaaaata acgggattct cgcctcccgc ccgcccgcag
     5881 aagcaggcgg gtatcatttt aaaacgcggc atttaaaatt tgaccgaaaa ttgttgacaa
     5941 tccggaatca agtctgcaca ataccccgac aagtccaagt attataaagg ctgaataaag
     6001 aggaaacagc aggcagatat attcgggagg tgcagtccga atatatctgc ttttttatgc
     6061 gcctccggat tgcctgccgc acctttccct tcagacggca tcagccgttt ccccataatg
     6121 ccgcccgatg cctatttatc tgccccggca atttcaaaac tgtgggtaat ctttgccgct
     6181 ttgcccaaca taatcgaagc cgaacagtat ttttcggcag acatctgaac ggcgcgctca
     6241 atggccgatt ctttcaaatc atgcccgaat actttgaaat ggatgtggat ttcggtaaac
     6301 acgcgcggcg catcgtccgc ccgtttcgcc gtaaccgtcg cacggcagtc agtcactttc
     6361 tgacgctgtt tttcggcaat catcaccaca tcgatgctcg aacagcccgc cacgcccaac
     6421 agcagcattt ccaaagggct gggcccgcgc ttggccgcac cttctgccgc cgacccctcc
     6481 ataacgacgc tgtgcccgcc ttccgttgtt ccgacaaagc acattccgtc tatccatttt
     6541 gatgtaacct gcatggtgtc attcctgaaa atagcgttaa aaccgctttg catatggcgt
     6601 tattgtaaac aatttcaagc ggcttatgca gaaatatgga caaaacggca aaaaaacgcc
     6661 tgaaaatcga tttacggttt ggctgcctgg ccgttgatct gcaccgattt gagtttcagc
     6721 gtataggttt tgccgtcgtc ggtatagccg atttgtgccg gaatattgtt cagggacggt
     6781 gcgaagaaat acattaccgc atcgtcgccg cgccgcaccc gatatttgac gacttcggtt
     6841 tccacgccgc ctatgctgta ttttcctgta cccgccttat tcaaaccgcc gacggaataa
     6901 agttttttgc cgttggtgat tttcagcccc ggggggagtt tcgcgtcatt tgccgccaac
     6961 tgccaggcaa gcgtgaacaa atccatagcc ttggggcttt gctcggtttt gctctcgccc
     7021 gctttgccgt aggttacgct gccgtcggcg aatttggctt ccgcatacag tttgcccctg
     7081 cgtatgtctc tatagtaggt agggtgcagg gtattgccga caaccgtacc gccggactcg
     7141 aaacggatat tgtatagcgg cactttaatc gtcgaaacga ttttgtaagc attgccgctg
     7201 cgttcaaatg tcatcgtggc gggaatgccg tagctgccgg aatagtgcag cacggcggat
     7261 tggggcagcc ctgccgcata cgcgcacggc agggcggcgg acaaaatggc ggcggaaaat
     7321 atatttttaa aagtcttcat catttgctcc cgcccggttt acgccgtcag gaaacgggcg
     7381 gcatcggcgt tttccgaatt tctgacgcgg tttccctcga taatcaggcg gccggcggca
     7441 aaatcggcaa cggctttcgg ataaagttta tgctcaacag ccaaaacccg ggcggcaaca
     7501 tcgtctgccg tatcgccgtc gagtatcggc acaatccctt gcgatacaat cgggccgcaa
     7561 tccagttcgg cagtaacgaa atggatggtg cagccggcaa cgcggcagcc cgcctccaaa
     7621 gcgcgttcgt gcgtatgaag tccggtaaac gagggcagga cggacgggtg aatgttcatc
     7681 agcctgcctt cgtaatgggc acagaactca ggggtcagaa tccgcataaa acctgccaaa
     7741 accaccaagt cgggttgata tgcgtcgatt ttttccatca tggcggtatc gaaggcaagt
     7801 cgtgattcaa agtttttatg attcaggctg tcggtcggaa taccctgacc ggccgcccat
     7861 tgcaaaccgg cagccgtttc gctgttgctc aacacggcgg caatacggac gttgggaatg
     7921 gcggcattga cgattgcctg catattgctg ccgcgtccgg aaatcaggat gacgatgttt
     7981 ttcataatgg tgcgcttttg aaagggatgc catctgaacc gctgtttggt ggtttcagac
     8041 ggcatttgcc gtaaaaatgc ccgaaaacct gtttcgggca tggattcgga cttaatttac
     8101 ttttttgatg tcgacttgag ccggctgctt ggcgggcgcg ttttcgggtg cgccgatttt
     8161 gaccagtttc acatcaaata ccaaagtggc gttcgggccg attttgtcgc ccgcaccctg
     8221 ttcgcggtag gcaaggttgg acgggatgta gaacgtggct tcgccgcctt ctttcagaag
     8281 ctgtacgcct tcggtccaac ccggaatcac ttggctcaaa gggaaggtga ccgggccgcc
     8341 gttggctttg ctgctgtcga ataccgtacc gtcaatcagg cggccttcgt attccacggt
     8401 aacgatgtcg tctttggtcg gctgtttgcc ttcgccctgt ttggtgattt tgtattgcag
     8461 gccggaagca gtggtcttca cgccgtcttt ggcggcattt tctttcagaa aggcttcgcc
     8521 tttttcttta ttggccttcg cgtccgcctt gtgtttttct acggctttag cctgttgttc
     8581 ctgaaggaat ttcatcatga cttcctgagc ctgctcttcg gtcattttga tttctttgcc
     8641 gtcatacact gcctgcatgg cttcggtaaa gactttcaaa tcgatttccg cgccctgttc
     8701 cttcatttgc ttcagggagc gtccgatgtc cacgcccatc gcatagcttg cctgctgcat
     8761 cgtgctgccg atcgaagagg tgtcgccctg cgcggaagaa gcggcggcag gttcggatgc
     8821 agatgcgggg gcggcttctt ttttgccgca ggcggaaagt gccaaagcgg cggaaagggt
     8881 cagtgcgctg attttgaaaa tggtgttcat gatggatctt cgctgtcgat aaggtcggaa
     8941 aaacgggatt atagccgagt ttgaatgttt caacacacag gatgacacat aaagcgtcaa
     9001 tcgtgtgttg ccctgttttg gaaaagattg aaccttccaa aataagtttt gattctaccg
     9061 ccccgaggga cagatgtcca agtggcgggg ttcaaccgat aaggaaattt taatcaaata
     9121 gaatcaagcc tgtttaaatt ttgtaaatgc ggcatttcag acggcatttt atgccttgcc
     9181 ctccatgccg tgatgttcga tggcaaaacc gcttcggcgg taggcggtaa agcgttcgcg
     9241 cgcgtcggcg agctcttcca ggctgttgcc gacgatttct aaaacgcgcg cgggcaagac
     9301 cggagcggtg ttccaaaaac cgtcggacag gttcaaaacg gccatgcctt cgggaattcg
     9361 gggcagattg ccgccgcagg caagcaggac ggatggttca gacggcatgg cttcttccgt
     9421 ttcccaaatt tcgtgcggaa taaaactctc ggcctcgtat tgccaaagca ttttgtccaa
     9481 ttcctgaagc tgcccgaacg aatcggacca caccagtatc ctgccgccgt cccgaatcgc
     9541 acgggcaatc aggcggcagg tgaaaatcgg aacttgggca acgtgcgtgt aaaaggtggc
     9601 tttcggcata ttgtttgagc atttggcagg acgatgccgt ctgaaaggct tcagacggca
     9661 ttgtggcaaa attaaagatt ccgcagatag ttcagcagca agggaacggg acggccagtc
     9721 gcaccttttt ccgctccgga tttccacgcc gtacccgcga tgtcaaggtg cgcccacggg
     9781 tagccttcgg tgaagtaaga caggaatgtt gcggcggtaa tcgtgcccgc gccgggcgtg
     9841 ccgatattgg ggatgtcggc aaagttggat ttgagctggt ctttgtaggt ttcaaagagc
     9901 ggcagttgcc acgctttgtc gtccacgttg taggaagcgg caagcaggct gtcgaccaaa
     9961 tcttgattgt tgcccatcac gccgctgaca tcgtgcccca aggcgacaat acacgcgccg
    10021 gtcagggtgg cgacgtcgat gacggctttg ggtttgaact gttcggcgta agtgagcgcg
    10081 tcgcacaaaa tcagacggcc ttcggcatcg gtgttcaaca cttcgatggt cagacctttc
    10141 atacttttca cgacatcgcc cggtttgttt gccgcgccgg aaggcatatt ttcacaagtg
    10201 gcgacgacgg caatcaggtt aatcggcagt tgcagtttga cggcggcgca gaaggtgctg
    10261 atgacggttg ccgcgccgca catatcaaac ttcatttcgt ccatgttcag gccgggtttg
    10321 agggaaatgc cgccggtgtc gaaggtaatg cctttgccga ccaataccac aggcgcggct
    10381 tctttgtccg ccgcaccgaa atagctcagt tcgaccaaat agggatcttc gacactgcct
    10441 ttggcgaccg accaaaacga acccatgttt tctttgatgt agtctttttc aatgattttg
    10501 gcgtgtgcgc ccaatttttc ggcttcggct ttggcggtgc gcgctaaaaa ttcgggcgtg
    10561 cattcgttgg gcgcggcgtt gcccaagtcg cggcagaggc tttgtccgta aacctgcgct
    10621 tcggcaacgc gcagggcttc tttgacggcg gcttcgtgtg cgctgtggaa cacggcttct
    10681 gcaaatttgg cgggcttggc ttctttttta tagcggtcga aacggtaggc ggcattgccg
    10741 aaagcaatcg caaacgcttc ggcaacggct gcggcttgcg cttcttcaaa ggcatgaacg
    10801 tccacattga ccgtttcctg attttgcgcc catttggcgg cttcggcagc ggccttgttc
    10861 aatgcggcgc ggccggtgct tttcagacag catacggcaa cagcctgcaa accgttgcct
    10921 gtcgggattt ttgtgtcggc aaaattttga ccttcttcaa gcgaagacaa aagggcaagg
    10981 gcggtcggat ggtttaattg cgcctcttcg gtgcagacaa ataactgtgc gcctgcctgc
    11041 tgttcctgca agatttcggt ttttgtgcta aattccacgt ttattctcct gattgagacg
    11101 gttgtcggta gttttcggac ggcctttcgc tcaaaagacc gtctgaagac ggctggcacg
    11161 attgtacccc atttgaagca ccgtctgaaa ccttgcgcgg acaatccgcc tgcgccgaac
    11221 cgcttaccgc cccctgaccg cgattctatg atttatcaaa gaaacctcat caaagaactc
    11281 tcttttaccg ccgtcggcat tttcgtcgtc ctcttggcgg tattggtctc cacgcaggca
    11341 atcaacctgc tcggccgtgc cgccgacggg cgtgtcgcca tcgatgccgt gttggcattg
    11401 gtcggcttct gggtcatcgg tatgacgccg cttttgctgg tgttgaccgc atttatcagt
    11461 acgttgaccg tgttgacccg ctactggcgc gacagcgaaa tgtcggtctg gctatcctgc
    11521 ggattggcat tgaaacaatg gatacgcccg gtgatgcagt ttgccgtgcc gtttgccgtt
    11581 ttggttgccg tcatgcagct ttgggtgata ccgtgggcag agctacgcag ccgcgaatac
    11641 gctgaaatcc tgaagcagaa gcaggaattg tctttggtgg aggcaggcgg gttcaacagc
    11701 ttgggcaagc gcaacggcag ggtttatttt gtcgaaacct tcgacactga atccggcatt
    11761 atgaaaaacc tgttcctgcg cgaacaggac aaaaacggcg gcgacaacat tatcttcgcc
    11821 aaagaaggca atttctcgct gaacgacaac aaacgcacgc tcgacctgcg caacggctac
    11881 cgttacagcg gtacgcctgg acgcgccgac tacaatcagg tttccttcca aaaactcaac
    11941 ctgattatca gcaccacgcc caaactcatc gaccccgttt cccaccgccg taccattccg
    12001 accgcccaac tgattggcag cagcaacccg caacatcagg cggaattgat gtggcgcatc
    12061 tcgctgaccg tcagcgtcct cctactctgc ctgcttgccg tgccgctttc ctatttcaac
    12121 ccgcgcagcg gacataccta caatatcttg attgccatcg gtttgttttt aatttaccaa
    12181 aacgggctga ccctgctttt tgaagccgtg gaagacggca aaatccattt ttggctcgga
    12241 ctgctgccta tgcacatcat catgttcgtc atcgcaatcg tacttctgcg cgtccgcagc
    12301 atgcccagcc agcccttctg gcaggctgtt ggcaaaagtc tgacattgaa aggcggaaaa
    12361 tgaacctgat ttcacgttac atcatccgtc aaatggcggt tatggcggtt tacgcgctcc
    12421 ttgccttcct cgctttgtac agcttttttg aaatcctgta cgaaaccggc aacctcggca
    12481 aaggcagtta cggcatatgg gaaatgctgg gctacaccgc cctcaaaatg cccgcccgcg
    12541 cctacgaact gatgcccctc gccgtcctta tcggcggact ggtctctctc agccagcttg
    12601 ccgccggcag cgaactgacc gtcatcaaag ccagcggcat gagcaccaaa aagctgctgt
    12661 tgattctgtc gcagttcggt ttgattttcg ccattgccac cgtcgcgctc ggcgaatggg
    12721 ttgcccccac gctgagccaa aaagccgaaa acatcaaagc cgccgctatt aacggcaaaa
    12781 tcagcaccgg caataccggc atttggctca aagaaaaaaa cagcattatc aacgtgcgcg
    12841 aaatgttgcc cgaccatacc ctgctgggca ttaaaatctg ggcccgcaac gataaaaacg
    12901 aactggcaga ggcagtggaa gccgattccg ccgttttgaa cagcgacggc agttggcagt
    12961 tgaaaaacat ccgccgcagc acgcttggcg aagacaaagt cgaggtctct attgcggctg
    13021 aagaaaactg gccgatttcc gtcaaacgca acctgatgga cgtattgctc gtcaaacccg
    13081 accaaatgtc cgtcggcgaa ctgaccacct acatccgcca cctccaaaac aacagccaaa
    13141 acacccgaat ctacgccatc gcatggtggc gcaaattggt ttaccccgcc gcagcctggg
    13201 tgatggcgct cgtcgccttt gcctttaccc cgcaaaccac ccgccacggc aatatgggct
    13261 taaaactctt cggcggcatc tgtctcggat tgctgttcca ccttgccgga cggctcttcg
    13321 ggtttaccag ccaactctac ggcatcccgc ccttcctcgc cggcgcacta cctaccatag
    13381 ccttcgcctt gctcgccgtt tggctgatac gcaaacagga aaaacgctaa accaatgccg
    13441 tctgaccccc ttcggacggc attggttttt attatcatat tcctgtagac agatagtcgt
    13501 tccctattat agtggattaa caaaaaccag tacggcgttg cctcgccttg ccgtactatt
    13561 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat acattacctg
    13621 tcataacagt tccatttttg ttaaaactag tctatgatag cggtacaaat attgtttaca
    13681 atatttaacg caaatcattt gcaacccgac aaaagaaaaa cagaaaaagg aacaaagaga
    13741 tgttagaagc ctatcgtaaa gccgccgccg agcgcgccgc cctcggcatt cccgccctcc
    13801 ctttgaacgc gcagcaaacc gccgatttgg ttgagctgct gaaaagcccg cccgcaggcg
    13861 aaggcgagtt cttggtcgaa ctgcttgccc accgtgttcc gcccggtgtg gacgatgccg
    13921 ccaaagtcaa agcctcattc ctggctgccg ttgccgaagg cagcgcgtcc agcccgctga
    13981 tctcccccga atatgcgacc gaactcttag gtacgatgct cggcggttac aatatccacg
    14041 ccttaatcga actcttggac gacgacaaac tcgcgcccat tgccgccaaa ggcttgaaac
    14101 atacgcttct gatgttcgat tccttccacg acgttcagga aaaagccgaa aaaggcaaca
    14161 aatacgcgca agaagttttg caatcttggg cagatgccga atggtttacc tcacgcgcca
    14221 aagttcccga aaaaatcacc gttaccgttt tcaaagttga cggcgaaacc aatacagacg
    14281 acctctcccc cgcgcccgac gcgtggagtc gtcccgatat tccgctgcac gcgctggcca
    14341 tgctgaaaaa cccgcgcgac ggcatcacgc ccgacaaacc gggcgaagtc ggtccgatta
    14401 aattgttgga agaactcaaa gccaaaggcc atccggttgc ttacgtcggc gacgtggtcg
    14461 gtaccggttc ttcacgcaaa tccgcgacca actccgtcat ttggcatacc ggcgaagaca
    14521 ttccgttcgt gccgaacaaa cgcttcggcg gcgtatgttt gggcggcaaa atcgcgccga
    14581 ttttcttcaa tacccaagaa gattccggcg cgctgccgat tgaagtcgat gtatctgctc
    14641 taaaaatggg tgatgtcgtc gatatcctgc cttatgaagg caaaatcgtg aaaaacggcg
    14701 agactgttgc cgagtttgaa ttgaaatcac aagtattgct ggacgaagtg caagccggcg
    14761 gccgtatcaa cctgattatc ggccgaggtc tgaccgccaa agcgcgcgaa gccctgaaac
    14821 tgcctgcctc tactgcattc cgcctgccgc aagcgcctgc cgaaagcaaa gccggtttca
    14881 ccttggcgca aaaaatggtc ggccgcgcct gcggtctgcc cgaaggacaa ggcgtgcgcc
    14941 cgggtactta ctgcgaaccg cgtatgacga cggtcggctc gcaagacacg accggcccga
    15001 tgacccgcga cgagttgaaa gacttggctt gtttgggctt ctccgccgat atggtgatgc
    15061 agtctttctg ccacaccgcc gcctatccga aacctgtcga tgtaaaaacc cataaagaac
    15121 tgcccgcctt tatttccacc cgtggcggcg tgtcactgcg tccgggcgac ggcgtcatcc
    15181 actcgtggct caaccgcctg ctgctgcccg ataccgtcgg caccggcggc gacagccata
    15241 cccgtttccc catcggtatt tccttccccg ccggctccgg cttggttgcc tttgccgccg
    15301 caacgggcgt aatgccgctc gatatgcccg agtctgtatt ggtacgcttc agcggcaagc
    15361 tgcaaccggg cgtaaccctg cgcgatttgg tgaacgccat cccgctgtac gcaatcaaac
    15421 aaggtttgct gaccgttgcc aaagccggta agaaaaacat cttctccggc cgcatcctcg
    15481 aaatcgaagg cctgcctgat ttgaaagtgg aacaagcctt tgaattgacc gacgcatccg
    15541 ccgaacgctc cgccgccggc tgtaccgtga agctcaacaa agagccgatt atcgagtaca
    15601 tgaaatccaa cgtcgtgttg atgaaaaaca tgattgccaa cggctatcaa gacccgcgca
    15661 ctttggaacg ccgcatcaaa gctatggaaa aatggctggc aaatcccgag ttgctcgaag
    15721 cggataaaga tgccgaatac gccgccgtga ttgaaatcaa catggacgac atcaaagagc
    15781 cgattatcgc ctgcccgaac gacccggacg acgtgtgctt catgtccgaa cgctccggca
    15841 ccaaaatcga cgaagtattc atcggttcgt gtatgaccaa catcggccac ttccgcgccg
    15901 cctccaaact tttggaaggc aaggcagaca cccccgtccg cctgtggatt gcgccgccga
    15961 ccaaaatgga cgcgaaacaa ttgtccgacg aaggacacta cggcgtactc ggacgtgccg
    16021 gcgcgcgtat ggaaatgccg ggttgctcct tatgtatggg taatcaggcg caagtacgcg
    16081 aaggtgcgac cgttatgtcc acctccaccc gcaacttccc gaaccgtttg ggtaaaaaca
    16141 cctttgttta cctcggttcg gcggaattgg cagcgatttg ctccaaactg ggtaaaatcc
    16201 cgaccgttga agaatatcaa gccaatatcg gcatcatcaa cgaacagggc gataaaatct
    16261 accgctatat gaacttcaac gaaatcgaca gctacaacga agtagccgag accgtgaacg
    16321 tttaatcccc gtcatccgta tgaagtaagg gattgaccgc aatgccgtct gaacaacctt
    16381 cagacggcat tgcaacattc cgctaaccct tctttccgca aacgctgcaa atacggcgtt
    16441 cacgccccca cataaaggaa acgacagtga acctgaaaaa ccgccatttt ctgaaacttt
    16501 tagacttcac gccggaagaa atcaccgcct acctcgacct tgccgccgaa ttgaaagccg
    16561 ccaaaaaagc agggcgcgag attcagcgga tgaaagggaa aaacatcgcc ctgatttttg
    16621 aaaaaacctc tactcggacg cgctgcgcgt ttgaagtcgc cgcgcgcgat caaggcgcgg
    16681 gagtgactta tttagagccg tccgccagcc aaatcgggca taaggaaagc atcaaagaca
    16741 ccgcccgcgt gttgggcagg atgtacgatg ccatcgaata tcgcggtttc ggtcaggaaa
    16801 ctgtcgaaga attggcgaaa tacgcgggcg tacccgtgtt caacgggctg accaacgagt
    16861 tccatcccac acaaatgctt gccgacgcac tgactatgcg cgaacacagc ggcaaacctt
    16921 tgaaccaaac cgcgtttgcc tacgtcggcg acgcgcgtta caacatgggc aattccctgc
    16981 tgattttagg ggcaaaattg gggatggacg tgcgtatcgg cgcaccgcaa agcctgtggc
    17041 cgtctgaagg cattattgcc gccgcacacg ccgccgccaa agaaaccggc gcaaaaatta
    17101 ccctgaccga aaacgcgcat gaagccgtga agaatgttga ttttattcat accgatgtgt
    17161 gggtcagcat gggcgagccg aaagaagtct ggcaggaacg catcgatttg ctgaaagatt
    17221 accgcgttac gcccgaactg atggcggcat cgggcaatcc gcaagtcaaa ttcatgcact
    17281 gcctgcccgc cttccacaac cgcgaaacca aagtcggcga atggatttac gaaaccttcg
    17341 ggctgaacgg tgtggaagtt acagaagaag tattcgaaag ccccgccagc atcgtgttcg
    17401 atcaggcgga aaaccgtatg cacacgatta aagcggtaat ggtcgcggct ctgggcgact
    17461 gacagaactg tgcctgttta aattcatccg caacacagat accgtctgaa cacgatgttc
    17521 agacggtatc catatatagt ggattaaatt taaaccagta cggcgttgcc tcgccttgcc
    17581 gtactatttg tactgtctgc agcttcgtcg ccttgtcctg atttttgtta atccactata
    17641 aaaaaactgc ctacacgatg tgtaggcagt cccgtttgaa aacaatcagt ttttgtcttg
    17701 gtcaaccaat ttgttggcag taatccaagg catcatggca cgcagttgtg cgccgacttt
    17761 ttcaacttgg tggtcggcat tcaaacggcg gcgggcagtc atagacgcgt agttgacgtt
    17821 accctcttgg ataaacattt ttgcgtattc gccggtttgg atgcgtttca gagcattgcg
    17881 catggcttct ttgctggaag cattgaccac ttcagggccg gtaacgtatt cgccgtactc
    17941 cgcattgttg gaaatggagt agttcatatt ggcaataccg ccttcgaaaa tcaggtcaac
    18001 aatcagtttc atttcgtgca gacattcgaa gtaagccatt tcaggcgcgt aaccggcttc
    18061 ggtcagggtt tcaaaacctg ctttgatcaa ctcgaccacg ccaccgcaca atacagcttg
    18121 ttcgccgaac agatcggttt cggtttcttc gcggaaagtg gtttcaatca caccgccttt
    18181 ggtgccgccg ttggcagccg cataagacag ggcgatgtct ttggccttgc cggaattgtc
    18241 ttggtaaacg gcaatcagag aaggcacgcc gccgccgcgt ttgtattcac tgcgtacggt
    18301 atgacccggg cctttggggg caaccataat cacgtccaag tcggcacgcg gaacgatttg
    18361 gttgtagtga acgttgaagc cgtgtgcaaa tgccagcgtt gcgccttctt tcagattggc
    18421 tgcaacttcg gcgtgataga cggcaggcat ggtttcgtca ggcagcagca gcataacgac
    18481 atcggcttct ttggtcgctt cagcaacagt tttgacgaca tggccggctg cttcggcttt
    18541 tttccaagaa gaaccttggc gcagaccaat caccacgttt acacccgaat ctttcaggtt
    18601 ggcagcatgg gcatggcctt gcgaaccgta accgatgatg gcaacggttt tgcctttgat
    18661 cagggacaga tcggcatctt tatcgtaata gacttgcatt tgatttcctt taaggtaaat
    18721 ggttgtcgaa gccttaaaat gttgagcggc ttcggacggg ttaaacagag tgtgccgctt
    18781 aagcggcaac ttcattcatc aatacgattt ccaacgcttc ggttttgccg tcgacggact
    18841 ggacgaaggc ttggaaatgc gcgctggcat tgtgttcgtc aatagctgct tgggatttcc
    18901 aattttccac gaaaacaaaa cggttcggtt tgccgatttc ctgatggaga tcatagctga
    18961 tgttgccctc ttccgcacgg ctggctttga ccagttcttt aaactgtgct gccagtgttt
    19021 ctgtgtattc cggtttaacg gtaaccagtg cgacaatttt aatgttcgac ataaatctct
    19081 cctgccgttc gtttttcaga cggcattcaa ataccgtgcc gtctgaaagg ttacggcgtt
    19141 aaattttcaa aatacgctca ccgcgaccga tgccggccgc gcctgtacgt acggtttcca
    19201 aaatttgggc gcgtccgacc gtttccaaaa aggaatccag cttgtctgtc gagccggtaa
    19261 tttcaatcgt atagctgcgg tcggttacgt cgatgatgct gccccggtag atttcggtca
    19321 agcgtaaaaa ttcgtcgcgg tctttgccgg cggcacggac ttttaccaac atcagttcgc
    19381 gttcgacaaa acggctttcg ttcaaatcga ccactttaat cacttcaatc aatttattga
    19441 gttgcttggt gatttgttcg ataacctgct cgtcgccgtg tgtaacgatg gtcatacggg
    19501 acagggtttt gtcttcggtc ggcgcaaccg ccaaagaatc gatattgtaa tcgcgtgcag
    19561 agaacaaacc gaccacgcgg ctcatcgcac ctgattcgtt ttcgatcaga acagataaga
    19621 tatgtcgcat ttgcctctcc ttacgccttt ccgtccgcac gcatatgcgg cggaagtacc
    19681 atttcgtcca aacctttgcc gttgccgacc atgggcatca cattctgttt ctggtcggtc
    19741 aggaagtcga taaacaccag cctgtctttt tggttcaatg cttccaacaa cgcaccttcc
    19801 acatcagact tcttgtccac gcggataccg atatggccgt atgcctcggc aagtttgacg
    19861 aaatcgggca aagaatcgaa ataggtttcc gactctcgtc cgccgtaata tatttcctgc
    19921 cactggcgta ccataccgag ataaccgttg ttcagcgtaa tgacgttaac cggaatccga
    19981 tattggaaac aggtggacag ctcttggatg ttcatctgga tcgagccgtc gccggtaata
    20041 cagaatacgt cttgatccgg ggcggcaagt tttgcaccaa tcgcataagg cagaccaacg
    20101 cccatcgtac ccaaaccgcc ggagttgagc cattggcgcg gacgttcgaa gggataatat
    20161 tgagccgcaa acatttgatg ctgccctaca tccgatgtga tgattgccga attgccggta
    20221 atctcggcaa gcttctgaat cacatattgt ggcttgataa tttcgctgcc gttgtcaaac
    20281 cacaagcaat cgcgggaacg ccattcctct atggttttcc accatttgcc caaagcatct
    20341 tcagacggca cggactcttg tttttgccac agcgcaacca tctcggacaa aacgtttttc
    20401 acgtcgccga caatcggaat gtccgccttc acgcgtttgg cgatgctgga aggatcgaca
    20461 tcgatatgga taaccttctt cgccttctcg aaaaatttgg acggtacgga aaccacacgg
    20521 tcgtcaaaac gcgcacctac ggcaagaacg acatccgcat tctgcatggc aaggtttgcc
    20581 tcgtaagtac cgtgcatacc gagcataccg aggaattggc ggtcgccgga aggataagcg
    20641 cccaagccca tcagcgtacc cgtgcacgga gcacccgtca ttcggacaaa tcgggtcagc
    20701 tcttcagaag cattacccaa caccacgccg ccgccaaaat agacgaccgg acgtttggca
    20761 gatgccaaca tctgcacggc ctttttaatc tgaccgatat gcccttgaac aaccggttga
    20821 tacgaacgga taaaaatgtc ttcctgagga tagctgaatt tcgccatcgc ctgcgtaaca
    20881 tctttcggga catcaaccac cacgggcccc ggtcggccgc ttgcggcaat ttggaacgcc
    20941 tttttaatgg tttccgccaa ctcattgatg tccgtaacca ggaaattgtg tttgacgcac
    21001 ggacgggtaa tacccaccgt atcaacttct tggaacgcat ccgtaccaat cagggaattg
    21061 cctacctgcc cgctgatgac caccatcgga atcgaatccg tataggcagt agcaataccg
    21121 gtcagtgcat tggtaacgcc cgggccggat gtaaccaatg ccacgcccac cttaccgctg
    21181 acgcgcgcat acgcatctgc cgcgtgtact gccgcctgct cgtgacgggt cagaatgtgc
    21241 ttgaatttat tgagttggaa aagggcatcg tagatttcga taaccgcacc gccgggataa
    21301 ccgaaaacgt actcgacacc ttcggctttg agactctgca ctatgatttg cgcgcctgat
    21361 agctgcataa cgacctcttt tatacggttt caaaccaata gggacaaacc gctttgccac
    21421 agcacctgta atgcaattcc accaagcagc gatttagggt acgcgcattg ggggaacacg
    21481 gcaacagacg gattatccaa tcaattggaa aggaacacag agtttgtgaa aaagagtaga
    21541 aacgataacg caaaccgaca gttcaatcaa gaaaaatctt tcatctttta attatttttt
    21601 gaaagcagag aaattattga ttgattttaa aagaataaaa tcaggagtac cttttttgaa
    21661 agatggaaat tgttgacagt ttgtgtagga ggggcagatg tgaaaaaccc ttcttcgata
    21721 tcaagaattg taaaatttac agggtttcat cccaataaag actcgggata ttgattgaac
    21781 ttgattttat ttttgatata tcaaaaatat tcccaaccat acttcctgaa aatggctcat
    21841 tgcaccggac tgtattggac ggcattaaca gaaccaagag ggctaacaac gacttaatat
    21901 attga
//
LOCUS       DAWFLT010000026        21171 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWFLT010000026 DAWFLT010000000
VERSION     DAWFLT010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21171
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.026"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..293)
                     /locus_tag="WGR74_001023"
     CDS             complement(<1..293)
                     /locus_tag="WGR74_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0905460.1"
                     /translation="MSTFFQQTAQAMIARHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELS"
     repeat_region   733..1758
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=799..834
                     /rpt_unit_seq="gttgtagctccctttctcatttcgcagtgctacaat"
     gene            complement(1822..2148)
                     /gene="cas2"
                     /locus_tag="WGR74_001024"
     CDS             complement(1822..2148)
                     /gene="cas2"
                     /locus_tag="WGR74_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEZ0905461.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     gene            complement(2141..3055)
                     /gene="cas1"
                     /locus_tag="WGR74_001025"
     CDS             complement(2141..3055)
                     /gene="cas1"
                     /locus_tag="WGR74_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEZ0905462.1"
                     /translation="MTWRSLLIQNGGKLSLQRRQLLIQQNGESHTVPLEDIAVIIIEN
                     RETLITAPLLSALAEHGATLLTCDEQFLPCGQWLPYAQYHRQLKILKLQLNISEPLKK
                     QLWQHIVRQKILNQAFVADETGNDLAAKRLRTLASEVRSGDTGNREAQAAALYFQALF
                     GEKFTRNDNNAVNAALNYTYAVLRAAVARALTLYGWLPALGLFHRSELNPFNLADDFI
                     EPLRPLADLTVIHLYEQGRLKTELTLGIKQHLIKILYYQTSIERQHFSTLAAIDKMIS
                     SFQAGVTDKNAKQLKLPEILPLKEYQYE"
     gene            complement(3122..6370)
                     /gene="cas9"
                     /locus_tag="WGR74_001026"
     CDS             complement(3122..6370)
                     /gene="cas9"
                     /locus_tag="WGR74_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEZ0905463.1"
                     /translation="MAAFKPNPINYILGLDIGIASVGWAMVEIDEDENPICLIDLGVR
                     VFERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGL
                     IKSLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLK
                     GVADNAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFSRKDLQAELILLFEK
                     QKEFGNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAER
                     FIWLTKLNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKG
                     LRYGKDNAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTD
                     EDITGRLKDRIQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYG
                     DHYGKKNTEEKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETARE
                     VGKSFKDRKEIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKC
                     LYSGKEINLGRLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFN
                     GKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVA
                     DRMRLTGKGKKRVFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQK
                     ITRFVRYKEMNAFDGKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFE
                     EADTPEKLRTLLAEKLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEG
                     VSVLRVPLTQLKLKDLEKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAG
                     NRTQQVKAVRVEQVQKTGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKG
                     ILPDRAVVQGKDEEDWQLIDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNI
                     NIRIHDLDHKIGKNGILEGIGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR"
     gene            complement(6648..10082)
                     /gene="dnaE"
                     /locus_tag="WGR74_001027"
     CDS             complement(6648..10082)
                     /gene="dnaE"
                     /locus_tag="WGR74_001027"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ0905464.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPVGAADVRIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNVHHAELNPEWLKNGDNSGLICLSGAHYGEVGVNLLNGNEDAARTAALKY
                     AAWFPDAFYMELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMNRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPGQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSRDDYLIKLSNKGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLEKAMETEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIVDVGKIPLDDQAAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILSPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESITSARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGTFDSIEPNRAMLLANIDLAMNNADQKAANTNQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLGR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRIMANQVMTLQTARERYARSLSLALAPHHDIGELVQLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            10366..10602
                     /locus_tag="WGR74_001028"
     CDS             10366..10602
                     /locus_tag="WGR74_001028"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905465.1"
                     /translation="MADADDQRIGQFVFEDVHQRQCGFVGEQDLQPAVGQDFFERIAA
                     YGVSDGIFTFTEQAGRDVPPVLRQFWVRRRQVLQ"
     gene            10547..10861
                     /locus_tag="WGR74_001029"
     CDS             10547..10861
                     /locus_tag="WGR74_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0905466.1"
                     /translation="MCRRFCGNFGYGGGRCYNNGLFLKGDIMEATVYLEDNEYIALCD
                     LLKLAGLAESGGQAKAFIAEGLVLRNGETETRKTAKIRGGEVIEFDGARLEIADGYDP
                     EA"
     gene            10839..11048
                     /locus_tag="WGR74_001030"
     CDS             10839..11048
                     /locus_tag="WGR74_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905467.1"
                     /translation="MDTILKHKAEALLGEPLLEEPVRPELWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWAEDENAV"
     gene            11341..12177
                     /locus_tag="WGR74_001031"
     CDS             11341..12177
                     /locus_tag="WGR74_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ0905468.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKRGISGAYDGALALAANKEMVSRTHVAEFLIRVGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKKLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RH"
     gene            complement(12229..13521)
                     /locus_tag="WGR74_001032"
     CDS             complement(12229..13521)
                     /locus_tag="WGR74_001032"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ0905469.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDQAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGLSTEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALESLPGLKEGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAQKHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVDIDTR
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(13577..15487)
                     /gene="pglD"
                     /locus_tag="WGR74_001033"
     CDS             complement(13577..15487)
                     /gene="pglD"
                     /locus_tag="WGR74_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ0905470.1"
                     /translation="MTLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSDEW
                     FDFANWQSFLLTALLTITLFIRMGLYRAVTRFVSFRILSTALAGSIVSAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATTSGVRTFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPEITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPNTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TAHPRIMTADEAMLPWHELSALLDRIRAACDRYDQQAIRTLLINAPTGFAPSDGICDL
                     LWVRETHRKNAV"
     gene            complement(15535..16710)
                     /locus_tag="WGR74_001034"
     CDS             complement(15535..16710)
                     /locus_tag="WGR74_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ0905471.1"
                     /translation="MLNTSLSPWPCFTQEEADAVSKVLLSNKVNYWTGSECRKFEKEF
                     AAFAGTRYAVALSNGTLALDVALKAIGIGAGNDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETIKAALTSNTKAIIVVHLAGMPAEMDGIMDLAKEHDLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTACRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVSELNARKVPCYQGSCSEV
                     YLEKSFDDTPWRPKERLKNAVELGDTSLMFLVHPTLTDDEIAFCKEHIEAVLAEATR"
     gene            complement(16829..16945)
                     /locus_tag="WGR74_001035"
                     /pseudo
     CDS             complement(16829..16945)
                     /locus_tag="WGR74_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(16939..17133)
                     /locus_tag="WGR74_001036"
     CDS             complement(16939..17133)
                     /locus_tag="WGR74_001036"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905472.1"
                     /translation="MTKYYSWGVNGFPPYVFDTIWKRWKLNGRGGGYRVRHPGRHDRR
                     GQSGKAAAAQKPQDRDGISD"
     gene            complement(17181..17867)
                     /locus_tag="WGR74_001037"
     CDS             complement(17181..17867)
                     /locus_tag="WGR74_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0905473.1"
                     /translation="MKTINPETTVIVFDLDDTLYSEYEYKLSGIRSVIDTVAALYPDW
                     NSDNLWRNIDPDGKDWLDKLCRHCGFNESEKQVLLWQYRLHRPTLTPYAPPDFLSELT
                     APFAARALITDGRSLTQRLKLEALGLSSLFDDILISEACSSEKPDSKRFRHLQDKYAD
                     KAGCFIYIGDNISKDFIAPNTLGWITIGLLPSGQNIHRHQPESFNTEYHPDFWVNSFQ
                     DLVPIIDSNT"
     gene            complement(17854..19431)
                     /locus_tag="WGR74_001038"
     CDS             complement(17854..19431)
                     /locus_tag="WGR74_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEZ0905474.1"
                     /translation="MSKAVKRLFDIIASASGLIVLSPVFLILAYLIRKNLGSPVFFIQ
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDSERLTPFGKKLRAASLDELPELWNVLK
                     GDMSLVGPRPLLMQYLPLYDNFQNRRHEMKPGITGWAQVNGRNALSWDEKFACDVWYI
                     DHYSFWLDMKILFLTVRKVFIKEGISAEGEATMPYFTGNQTNEKNNILILSAGRRVEL
                     VQDFQTEAARFSDGISVFATDLNPRMSSACHVSDGSFEVPPISADSYIDSIFNLAVRE
                     NIGLIIPTIDTELQKLADERERFEAASVHIVVSDSDFIAQCRDKRKTATLFARYGIRS
                     PEIYDRKKLVFPCFAKPYDGSRAIGAKRIDTPADLTPEVLADTKLMFCQFIDIENKFS
                     EFTVDMYYDRQCRLKCAIPRKRLEVRSGEISKGVTCKNSLYQTLLEKMAVLQGARGCI
                     TAQFFYNEETGEFYGVEINPRFGGGFPLTYAAGGNYPSRLMREYLGDEEIPFSDDWEN
                     NLIMLRYDAKVLVHEND"
     gene            complement(19424..20582)
                     /gene="pglH"
                     /locus_tag="WGR74_001039"
                     /pseudo
     CDS             complement(19424..20582)
                     /gene="pglH"
                     /locus_tag="WGR74_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminyl-a1,3-N,
                     N'-diacetylbacillosaminyl-a1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglH"
     gene            complement(20602..21138)
                     /locus_tag="WGR74_001040"
     CDS             complement(20602..21138)
                     /locus_tag="WGR74_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0905475.1"
                     /translation="MLGTLSRLDTVRAVHLMLDIFKKMVDRDMPVRLNVAGIGEEMDN
                     LKAQAERLGIDGKVTFLGGIRDLTAYFKDVDILVNTPHCIGDHGAGVGNNILEAGLYD
                     TPVVTYDMAGISEMVVNGETGYCIPFGDEEAFIAAVDTLVRQPELREAMGKRLHAHVA
                     ALCSDDEIYRTTMAAYTM"
BASE COUNT         4440 a         5153 c         5991 g         5587 t
ORIGIN      
        1 ctgctcagtt cgtcgaaacg gcaaaacagg ttgaaatcga tgcgggtaat gaggctgtgt
       61 tcgagttcgg gatcggagag gctgtgccat tgtccgagca gaacggcttt gaacatggac
      121 aacagcgggt aggcgggacg gccgcggtgg tctcgaaggt aacgggttct ttgacggttc
      181 aggtattgtt cgatcggttg ccaatcaatc acctggtcca acttcaatag cgggaaacgg
      241 tcgatgtgtc tggcaatcat ggcttgggcg gtttgttgaa agaaggtgct catgagaaat
      301 cccctaaatg tcttggtggg aatttagggg attttgggga attttgcaaa ggtctcattt
      361 tgcaagattt tagtttaatc atcaaacagg gaacagcaaa aatccccgct gcaacgggga
      421 tttttgttcg gctctcgcct gtgtgattaa cttcactagg agaagctaaa atgcttaaat
      481 tccttatgcg tgggaaaaag ttgctgctgt ccgttgaagt gaaccacaag ttcattttag
      541 cggttgcttt gctgttaagt caatgcagta gctaacccga ccacaatcaa aaaaacgcaa
      601 gttttccgcc tactctgtga gtgggcggtt ttttcttatt tcaaatctgt gacacaatat
      661 ctgtcgcctt tcttgccata attgcactgt gtctcaatga ctttaacgca cgttcgctta
      721 tcgcaacggc tggttgtagc gcccattctc atttcgcagt gctacaattt tttgtatcca
      781 ttcccagccg gaaattaagt tgtagctccc tttctcattt cgcagtgcta caatctttaa
      841 ctttgaccgt gtgcaatcca gttagttgta gctccctttc tcatttcgca gtgctacaat
      901 ctgaccacac ctatccgcca gtgcttatgc gttgtagctc cctttctcat ttcgcagtgc
      961 tacaataagc cggtttatgg caattctgcc gcgtccgttg tagctccctt tctcatttcg
     1021 cagtgctaca atatgccttt ttttgtactg ttgtttgaac gagttgtagc tccctttctc
     1081 atttcgcagt gctacaataa attcgtttca gatagcaaac gcagtagtgt tgtagctccc
     1141 tttctcattt cgcagtgcta caatgccgga tatgacggtg ggcaactggt acaggttgta
     1201 gctccctttc tcatttcgca gtgctacaat atttcgcttt tgtattgatt caaggtgctt
     1261 gttgtagctc cctttctcat ttcgcagtgc tacaataaat tcgtttcaga tagcaaacgc
     1321 ggtggtgttg tagctccctt tctcatttcg cagtgctaca atagtattcg tcgatgatgg
     1381 aaactcgagc atgttgtagc tccctttctc atttcgcagt gctacaattt tctgttcttc
     1441 gtggatatac tggaatttgt tgtagctccc tttctcattt cgcagtgcta caatcttccg
     1501 cgcttccggg ggttaagtcg ggacgttgta gctccctttc tcatttcgca gtgctacaat
     1561 cttcatctct gcaaccatgt tgtccagcat gttgtagctc cctttctcat ttcgcagtgc
     1621 tacaataaac gcggcttttc ggggttttta ccttgggttg tagctccctt tctcatttcg
     1681 cagtgctaca atcttccgcg cttccgggtg tgaggtcggg acgttgtagc tccctttctc
     1741 atttcgcagt gctacaatac ttcgacggga aatccttatt tcataagggt ttcccgtttt
     1801 atttgttatg aaaaaatggg cttaaaataa caataattgg tctgaattga cctttttttc
     1861 ttgggttttc agctcgccca acagcagttt catggcggca tattgctttt ctgttatctc
     1921 caaacagcgg attgagcctt cttgcggaag gtttgcacac agtctgttgt ggtgtttttg
     1981 caacgaatcg cggcctttga cgatacggct gtatacggaa agttgcagca tttggtatcc
     2041 gtcttttaat aaaaactggc ggaattgatt ggcggctttg cgctttgccg ccgtgataac
     2101 cggtaggtcg aagaagacga taatcctcat aaatttggcc tcactcatat tggtattctt
     2161 tcaatggtag gatttcaggc agtttcagtt gtttggcgtt tttatcggtt acgccggctt
     2221 gaaatgacga aatcattttg tcgatggcag ccaaggtgct gaaatgttgc cgttcgatgc
     2281 tggtttgata gtagaggatt ttgatgaggt gttgtttgat gccgagcgtc agttcagttt
     2341 tcagacggcc ttgttcgtac aaatgtatca cggtcaggtc ggccaatggg cgtaagggtt
     2401 cgataaagtc atcggcgaga ttaaatggat tgagttcact tctatgaaac aagcctaatg
     2461 caggtaacca accgtataag gtcagggcgc gtgcaactgc ggcacggagt acggcgtagg
     2521 tgtagttcaa tgcggcattg acggcgttgt tgtcattacg ggtaaatttt tcgccaaaga
     2581 gggcttgaaa gtagagggcg gcagcttggg cttcacggtt tcctgtatcg cctgaacgga
     2641 cttcagatgc caaagtgcgc agccgtttgg ctgcaagatc attgcctgtt tcgtctgcga
     2701 caaatgcttg gttcaggatt ttttggcgga cgatatgttg ccagagttgc ttttttaagg
     2761 gttcgcttat gttcagttgc agtttgagga ttttaagttg gcggtgatat tgggcataag
     2821 gcagccattg tccgcagggc aggaactgct cgtcgcaggt cagcagggtt gcaccatgct
     2881 cggctaaggc tgataggagc ggggcggtaa tcagggtttc gcgattttcg atgatgataa
     2941 cggcgatgtc ctctaatggt acggtgtggg actcgccgtt ttgttgaatc agcagttgcc
     3001 ggcgttgcaa ggatagtttg ccgccgtttt gaataagcag gctgcgccaa gtcatgtttt
     3061 tccccttatt tttatataaa aaatccctgc ttcaggccgc ctgaaacagg gatagattaa
     3121 tttaacggac aggcgggcgt tttttcagac ggcatggtct gatttctttg cccagttcgt
     3181 caatttggta tttttggaat gaaagggcgg ttttgacgcc gataccttcc agtattccat
     3241 ttttgccaat tttatgatca agatcatgaa tgcgtatatt gatattaccg gtgcctcgat
     3301 ggcagctggc aaagtaacca aacattctag ctttttttgt tataacctcg actaaatcat
     3361 tagggtgtaa tgagaattta aagttgaaac tatcatcaat aagttgccaa tcttcttcat
     3421 cttttccttg tacaacagcc ctatccggca aaatcccttt cgctacctgc caactgtaaa
     3481 tcggtaccag ataatacttg tcgcctttct caaacacatc tacgcgcacc atggttgcgt
     3541 tgtcggcaat accgttatgg ttgcgcaccc atacgccggt tttctgtact tgctctacgc
     3601 gtacggcttt tacctgttgg gtgcggttgc ctgctttatc gtatttgtaa aacggctcgg
     3661 caaaggcttt ggcaggatcg tctttatgtg cttccagccg tgctttcagt gcttcgtata
     3721 gcttaggttc gcgctcccga ttgaccattt tttccaagtc tttcagtttt aactgtgtta
     3781 acggtacgcg caacacgctg acgccttcgt ccagtcgttt ggcggatttg acggtctcca
     3841 tatgcccttg cccgctcatc ttccgattgg gcgcgcgtga aacaaacagt ggcgtaacgt
     3901 attcgtgtac ggcttcgggg cgagatgata atttttcggc aagcaacgtg cgcagttttt
     3961 ctggggtatc ggcttcttcg aattcgggtt tgccgtccgg tttgccgaag acgcgaatca
     4021 tgacttcttg tgcgaaaaat tcccaaggtt gtgggaagtg tgttttttga tgcagcactt
     4081 ctcctgtttc tttgtctatg gttttaccgt caaacgcgtt catctcttta tagcgtacaa
     4141 aacgggtaat tttctgctgc atggcaacgg tcgagcaggc aacgacgacg gcgtccaagg
     4201 cgtgatggcg gtcgttttcc gcacgcactt tgcgcaatcc ccaaaagccg cgcaacagat
     4261 tggtaatttg tccgttggat gcaaagacac gtttcttgcc tttacctgtc agccgcatac
     4321 ggtcggcaac aaattgacac aggaaacggt tgacgtagcg cgtgtcgttc agattgcgtt
     4381 ctttaaagcc gtcttcatcg aatttttgca gcagaatccg ttgtttttta ctgcgcggga
     4441 aacggctggt ttcgacacgc gctttaaatt cctgccattc gcggctgttg tctttgccgt
     4501 tgaagtattc gtaaggggtt tgattgcctt tgttttggtt ttcgctgccc aataccagta
     4561 ctttattgtt gaaactgtcg tcccatgtgc gcgagaacgg cagggcatgg tcgatttcga
     4621 catagccttt ttcgttcaga cggcctaagt tgatttcttt gcccgaatac aggcatttgc
     4681 cgtgttgttg ctcgtacaga cgcagtttca gaatatcttt ggatttgggt tctccgacaa
     4741 aattggggaa atactctcgg aatttggcgg cggctttttc cctgtctttg cggttttctt
     4801 cttggcgttt ctcaatttct ttgcggtctt taaacgattt acctacttcc cttgcagttt
     4861 caatatggat acgagctggg gagccgtaac ggcgtaccac gccgttaatg accttacgtg
     4921 cttgagataa ggcgcgcaag acgacggggt tgcggatttc gtcggcggga atcggcggca
     4981 gataaatctt ttcttccgta ttcttcttgc cgtaatggtc tccgtagatt tcggcgcagg
     5041 cttcatcgta acgtttgcct tgttccatta gaggcacaat tcggcgcaat gctttcaagg
     5101 aaatttggac gaacttatcg aagctgatgt gtttcaacag cgcttctaag atttcgggct
     5161 gtatacggtc tttcagacgg cctgtaatgt cttcatcggt tttgaacagg gagaatgccg
     5221 tgccgatttc gtcttgtaat tcgggagaaa ggtttaatgg ggattttttg tctttcaatc
     5281 cttctttttc cagtgcacgg ctgatggcat ggtaggcctt catttccatc aatgttgagg
     5341 cttcggcatt gtctttacca tagcgcaagc ctttgaaaaa ggcggtatct tctaaaccca
     5401 gcagcttacg ggcttgtgcg taagtcagtt tggattttct gtatggctcg tccataagcg
     5461 tggcgcgttc ggtatcggtc aatggccgct cgctgccttg ctctaaaata cgcaggttgt
     5521 tcagcttggt cagccagatg aaacgttcgg ctgtgtaggt gtttttagcg gctttcggct
     5581 ctgccggttc gaaggtgcaa tgccccaaca ttttttgaac ggcatcgccg gacagggcag
     5641 ggcgttgcgt catcagtagg gtttcaatac cttctttaag gccgcctgaa acatgcggat
     5701 tgccaaattc tttttgtttt tcaaacagca aaatcagctc cgcctgtaaa tctttgcggc
     5761 tgaacgtatg cgaataatcg ccgcgctgat tgcggatatg gccgctttct ttctcaaatt
     5821 tatttaaagc caattcggcc ggtgtgcgga aatcgcctgt ctgtaaggca tgggcattgt
     5881 cggctacgcc tttgagcaaa gcgccaagct ctttatcggc agtttcgccc tcgttttttc
     5941 tttgcgacaa ataaccgcgg tgtttgatta aatgcaacaa gactgccgac cactctaaag
     6001 gcgtcagttt gcggtctaat gcggctgcgc gaagttgcca tggtgtattc ggtaaggatt
     6061 tgatcaagcc gttttcgtca aaatcagcgg cttgtaatac gccttcgcgt ttcaataggc
     6121 ggcgggcccg aagcaggcga tgggcgcgac ggcgggtcag gcggcggaca ctgcgcgcca
     6181 aacgccttgc catggcaagg gagtcgcctg ttttcggtac ttcggcacgc tcaaatacgc
     6241 gcacgcccaa atcaatcagg cagattgggt tttcatcttc gtcaatttct accatcgccc
     6301 agccgacgga tgcgatgccg atatcgaggc cgaggatgta gttgattgga ttaggtttga
     6361 aggcagccat ttattactcc gtaaacaacg ttgcaaataa tgataatata tcttattgtc
     6421 ttatttatat acaaatagat tattgactta tcatctcaca acggctagaa tcccaaacat
     6481 attgtcgcac tgcgaaatga gaaccgttgc tacaataagg ccgtctgaaa agatgtgccg
     6541 caacgctctg ccccttaaag cttctgcttt aaggggcatc gtttatttcg gttaaaaaat
     6601 gccgtctgaa accgattttg ggcttcagac ggcatttata ttttgggtta ccagttgacg
     6661 cgcaccgacc ggctgccgag caatgtttcc agttcgccga acaatgcgga gctcggtgta
     6721 accgtccatt tcggcggcac ttgaagcctg cccgacgctt tttcgttggc atacgacagt
     6781 tgcagcggga tgcgcggcgt gtcgggcagt tggtgggcgg caagcagttg taccagttcg
     6841 ccgatgtcgt gatgcggggc gagggcgagg ctgaggctgc gggcgtagcg ttcgcgtgcc
     6901 gtttgcaggg tcatgacttg gtttgccatg atgcgcagtc cgtcgccgcc gccgtagtcg
     6961 tcgcggctga ctttggattc gataatcagc acttggtcgg ctttgaggca gtcggcgcag
     7021 ttttccagcg tctgaccgcc gaccatgatt tcaacctgcc cgctcaaatc ttcaaggctg
     7081 acgaaggcga ttttgccgcg cttgcccatc atcgtacgca cggcggtaac gaatccggcg
     7141 aggcgcacgc tgtcttgcgg cttcagacgg cctaatttgg tcggtgcgat ttggcggact
     7201 tcttgggcat acgggccgaa cgggtggccg gagaggtaaa agccgatgac ggttttttct
     7261 tcggcgagtt tttccgattc gctccacatc ggcgcgtcga tgagccgcac cggttcgatg
     7321 gcgtcttcca tcatgtcgaa aagtccgccc tgattggtgt tggcggcttt ttggtcggcg
     7381 ttgttcatgg cgaggtcgat gtttgccaag agcatggcgc ggttgggttc gatgctgtcg
     7441 aacgtgccgc cgcgtatcaa ggcttcgagg gtgcggcggt tcatgtgttc tttgccgacg
     7501 cgctcgcaga agtccaagag gccggtaaac ttgccgccgc tttgccgcgc ggaggtgatg
     7561 gattcgacgg cggcttcgcc cgtgccttta atcgcgccga gcgcgtagcg gattttcatg
     7621 tccggatacg gtgtgaagcg gtagtcggat tcgttgatgt cgggcggcag gaactcgata
     7681 ccgttggcgc ggcagtcgtc gtagaaatgc ttgagctggt ctgtgttgtc caattcagac
     7741 gacatggttg ctgccataaa ttcggcgggg taatgggctt tgagccatgc ggtctggtag
     7801 gaaatcaggg cgtaggcggc ggcgtgggat ttgttgaaac cgtagccggc gaatttttcc
     7861 atgtagttga agatttcgtc ggatttttcg cgtgaaatgc cttgttttgc cgcgccttcg
     7921 gcaaagattt cgcggtgttt caccatttct tcgggctttt tcttacccat ggcgcggcgc
     7981 aacaagtccg cgccgccgag cgagtagccg ccgataatct gcgccgcctg cattacttgt
     8041 tcctgataca ccataatgcc gtaggtcggt gagaggatgc cttccaacag cggatggatg
     8101 tattggaatt cctgtccctt catacgcgcg acgaagtcgg gaatattgtc catcgggcca
     8161 ggacggtaga gcgacacgaa ggcgatgagt tcttcaaact tggtcgtatg cgccgttttc
     8221 agcattttct tcatgccggt cgactcgaat tggaagacgg cggtggtgtt cgcatcgcgg
     8281 aagatttggt aggcggcctg gtcgtcgagc ggaattttgc cgacatcgac gatatcgccg
     8341 gtggtgtttt tgatgttgtt ctgcgccatt tcgataatgg tcaggttgcg cagacccaaa
     8401 aagtcgaatt tcaccaaacc cacgtcttcc acgtcgccct tgtcgtacat ggatacgggc
     8461 gaggcggatt cgtccgcctg atatacgggg ctgtaatcgg aaatcttgcc cggcgcaatc
     8521 aacacgccgc ccgcgtgcat acccaaaccg cgcgtcaggt cttccagctt tttcgccagc
     8581 gtaatcagtt cgtccgcttc ttccgcttcg attaattcct gaatctgtgg ctcggtctcc
     8641 atggcttttt ccaaactcag gggtttgttg gcttccaacg gaatcagctt ggacagtttg
     8701 tcgcacagca taaacggcag ttccagtacg cgccccacgt cgcgaatcac tgctttggac
     8761 gacatcgtgc cgaaggtaac aatctggctg actgcttccg caccgtattt ttcgcgtaca
     8821 tattcaatca cgcggccgcg gtttgcctga cagaagtcca cgtcgaagtc gggcatggaa
     8881 acgcgttcag ggttcaagaa acgttcgaac agcagcgcgt atttgagcgg gtcgaggtcg
     8941 gtaatcttca gcgaatacgc caccagcgag cctgcgcccg aaccacggcc cggccctact
     9001 gggcagccgt gcgttttcgc ccagttgata aagtcttgta cgataaggaa ataaccgggg
     9061 aatttcatct ggatgatgat gttcagctca aaatccaaac gttcctgata ttccggcatt
     9121 ttcgccgccc gctccgcctc gtcgggataa agctgaacca tgcgttcctg caaacccttg
     9181 tttgagagtt tgatgagata gtcatcgcgc gataaaccgt cgggcgtggg gaaaaggggc
     9241 aggaagtttt tgcccaatgt gatgtgcagg ttgcagcgtt tggcgatttc caccgtgttt
     9301 tccaaggctt cgggcaagtc ggcgaaacgc tccagcatcg tttccggcgg aatgaaaaac
     9361 tggcccggcg tgaaatcgcg cggacgtttt ttgtccgtca atacccagcc gcccgcgata
     9421 cacacccgcg cctcgtgcgc gttgaaatcg tcgcggttca taaactgcgt cggatgcgtc
     9481 gccaccaccg gcaaacccag ttcctccgcc agcttcacgc tgccggaaac gcaagcctcc
     9541 cattcggggc gttcgggcag gcgttgcagc tccatataga acgcatcggg gaaccacgcc
     9601 gcatacttca acgccgccgt acgcgccgcg tcttcattgc cgttcaacag attcacgccc
     9661 acttcgccgt aatgcgcgcc gctcaaacaa atcaagccgc tgttgtcgcc gtttttcagc
     9721 cattcgggat tgagttccgc gtgatggaca ttacggtctt tgccgacata ggccgccgtc
     9781 aaaagctcgc tcaagcgcag atagcccgca tcgttgcgga taatcagcat agcgcggaac
     9841 ggcttgtcgg gcgcattcgg attgcctatc cgcacgtccg ccgcgccgac gggcttaatc
     9901 cccgcgctgc ggcaggcttt atagaatttc accaaaccaa attcgttcat caaatcgctg
     9961 atgcccaaag caggcaaacc gtattcctgc gctttggcaa tcagtttttt aatccgcacc
    10021 ataccgtcgg taatcgaaaa ctcggtatgc aggcgcagcg ggatgtaggt cggctcggtc
    10081 atggcaaaat cggcgtgaac aataaaaggc gtattgtagc agggttgtct ttagatggcg
    10141 gtgtaggtaa tgctgtttca gattcagacg gcatgacctg caaatgtttt tgagctttta
    10201 cgacggcaaa aaaatgcctc ctgccgtatg gcggaggctt cccaaggagt attgatagat
    10261 ataaaggact atcaaactag ttataaggaa ctatatgcct tattcggacg gatggcaagc
    10321 agttaaatta attttacgtt caaacagatt tttgatttcg ttttgatggc tgatgccgat
    10381 gaccagcgta tcgggcagtt cgtgtttgag gatgtgcatc agcgtcaatg cggattcgtc
    10441 ggggaacagg atttgcagcc tgctgttggg caggatttct ttgaacggat tgcggcatat
    10501 ggggtttcag acggcatttt tacttttacg gaacaggcgg ggcgggatgt gccgccggtt
    10561 ttgcggcaat tttgggtacg gcggcggcag gtgctacaat aacggcctct ttctgaaagg
    10621 ggacatcatg gaagccactg tctatctcga agacaatgaa tacatcgcct tgtgcgacct
    10681 cttgaaattg gccggacttg ccgaaagcgg cgggcaggcg aaagcgttta ttgccgaagg
    10741 gctggtgttg cgcaacggcg aaaccgaaac ccgtaaaacc gccaaaatac gcggcggcga
    10801 agtcatcgag tttgacggcg cgcgcttgga aatcgccgat ggatacgatc ctgaagcata
    10861 aagccgaagc ccttttgggc gagccgcttt tagaagaacc cgtccgccct gagttgtggg
    10921 aatgctgcgg cagcgactgc ggcgaggcgt gcattcagac gatttactgg gcggataagg
    10981 cacgctacga cgcgcaacgg cggaagttga aagaagcggg ttgggcggaa gacgaaaatg
    11041 ccgtctgaaa ccagttcggc ttgataaaac cacgttttca gacagtcttt tatagtagat
    11101 taaaattgaa aacggtcata tcgccattcc ctcgaaagca ggaatccgga agtttgaaat
    11161 agcggttaac cttaaacatt tccaataaat caaagtctgg attcccgcct gcgcgggaat
    11221 gacggcatag cggcttttcc tttgcatttg ccatacactt gttttttgaa atcaccgccg
    11281 ccacaccgcc cgacaatatt gaaacaccct gcccaccatt taccacgcac ataacgcctt
    11341 atgatagacc tgcactgcca ttccaccgtt tccgacggta tgctctcccc cgccgaagtc
    11401 gtgcgcctcg cgcatcaaaa cggctgcacg ctgctggcgt tgaccgacca cgaccacacc
    11461 ggcggcatcg ccgaagcgcg tgccgaagcc gacaggctcg gtttgcgcct gattaacggc
    11521 gtggaaatct ccgtaacgtg gcgcgggcgt accatacacg ttgtcggctt agactttgac
    11581 gaacaagacg aaaacctgca aaacctgttg gcgcaagtgc ggcaaggccg tctgaaacgc
    11641 ctcgaagcca tcgccgccaa gctggaaaaa cgcggcattt cgggcgcata cgacggcgcg
    11701 ctcgcgctgg cggcaaacaa agaaatggtc agccgcacac acgtcgccga gttcctcatt
    11761 cgggtgggac acgtcaaaaa caagcagcag gcgttcacca aatacttggg cgacggcaaa
    11821 tcctgcgccg tccgccacga atgggcgacg ctggaagact gcgtctccgc cgtcaacggc
    11881 gcgggcgggt tggcggtcat cgcccatccg atgcgttatg atttgtccgc caccgccaag
    11941 cgcaatctgt ttgaagaatt taaaaaactc ggcggtgcgg gtatcgaagt ccacagcgga
    12001 aactgttgca aaaacgaccg cctcaactac gcgcttttgg cagaacgttt cggtatgctc
    12061 gccagcgcgg gcagcgactt ccaccgttta aacgacttca gcggcggcat cctcggcgcg
    12121 tgtcccgagc tgccggaaaa ctgtaggccg gtgtgggcgc atttctcccg acattgaatg
    12181 cggataaaaa tgccgtctga aaggctttca gacggcattt tttgcggttt aaacgttgtc
    12241 gtacagtttt cggacggttt taccgatggc ggcaatgcct ttttccagcg tttgagcgtc
    12301 ctgcgcgatg ctcatgcgga tgcactcgcc cgcgtgcgga taatcccgcg tgtcgatgtc
    12361 gacgaagaaa tgctcgcccg gaataatcag cgtaccttcg gctttgagca tttcgtacag
    12421 ggtttgcgaa gaaacgggta ggttttcaaa ccagagccac aggaaaatcg cgccttcggg
    12481 tttgtggatt ttcatcgggt acgcgcccag ctcgcgcttg agcagcgaga cggcggtttg
    12541 cgcctgattg cggtaaaacg gccggatgac ttggtcggca agccgtttca tctcgccgct
    12601 ttccagtaac ggggcggcga tggccgcgcc gaagcgcgtg ggggcaaggt tcacaatcgc
    12661 gttcaggctg ctgacggctt tgaccacttc cggcgcggcg acaatgatgc cggtgcgaac
    12721 gcccggcagg ccgactttgg aaaggctgaa gcagaggatg atgttttcgt gccaattcag
    12781 cgttacgtcg ctgtaaatga tgttggggaa cggcattccg taggcgttgt cgataatcag
    12841 cggaatgccg tgtttttgcg ccaaagcgtc cagccgcgcc atttcgccgt cggtcagcac
    12901 gttgccggtc gggttggtcg ggcgcgaaca gcaaatcgcg ccgattttgc cttctttcag
    12961 cccgggcagg ctttccagcg cgtcaaagtc cacgcggtat ttgaagaagc ctgcttcgcc
    13021 ttcgtgttcg acgttttcga ttttgggttt gacggaaacg aagtgctgcc cttcgacatg
    13081 cacgtcggca tagccgatgt attcgggcgc gagcggcagc aggatggctt tttcggtcga
    13141 gaggccgtct gaaagcttga atttaccgcc gaagaggttg aataaataga aaaacgcgtt
    13201 ttgcgaaccg ttggtcagcg cgatattgtc ggcggtcaga ttccagccgt attcgcggtt
    13261 gaggaaagcg gtcagcgcgt caatcagtgc ggcatcgcct tgggggttgg agtaattgcc
    13321 gatgttttcg acggcgtgtt ctgccgccag tttggagaat atgtcggcga acgcctgatc
    13381 gatttccgga atgcgcgccg ggttgccgcc gccgagcatg ttgacgggct tgtcgctttt
    13441 gagcgcgtcg ccgaggtcgt ccatcagttg gaggatgccg ctgtgttgcg tgaatttttc
    13501 gccgaatgct gagaactgca tgtcaaactc cgtgtgtgta aggtaggttg gtttgtacgt
    13561 tatgccgtct gaaggttcag acggcatttt ttctgtgtgt ttctcgtacc caaagcaggt
    13621 cgcagatgcc gtcgctcggg gcaaagccgg tcggggcgtt gatgagcagg gtgcggattg
    13681 cctgctggtc gtaacggtcg caggccgcac ggatgcggtc gagcagggcg gagagctcgt
    13741 gccacggcag catcgcctcg tcagccgtca taatgcgcgg atgggcggtt ttgcggacgt
    13801 tgtcgccgat gagcagctct tcgtagagtt tttcgccggg acgcagtccg gtaatgagga
    13861 tttcgatgtc gccgtcgggt tgttcgggtg tgttgggttt gaggccgctt agggtaatca
    13921 tttggcgggc aaggtcgatg attttgacgg attcacccat gtcgaggacg aatacgtcgc
    13981 cgcccgtacc catcgcgcct gcctgtatga cgagttgggc ggcttcgggt atggtcatga
    14041 aataacgtgt gatttcgggg tgggtcaggg taagcgggcc gccttctgca atctgttttt
    14101 caaacagcgg gacaacggag ccggacgaac ctaaaacatt gccgaaacgt accatgctga
    14161 agcgggtttt ttgtccgggt tcggcggcga gtgcctgaag gcagagttcc gccatgcgtt
    14221 tgctggcacc catggtgttg gtggggcgga cggctttgtc ggtggagatg aggacgaaag
    14281 ttcttacgcc cgatgtcgtg gcggcaagcg cgcactcgag tgtgccgaag atgttgttgc
    14341 gtatgccttc gacggtgttg aactcgacca tggggacgtg tttgtaggca gcggcgtgat
    14401 agacggtcgc aacggaaaag gcggtcatga cgtgttcgag cagcgtgcgg ttttgcaccg
    14461 aaccgagaaa gggcaggatt tcggtgtcga ggcgtttttg gatgcaggtt tcgcgcaatt
    14521 ctttttcgat ggcgtacagg gcgaattcgg ataactcgaa cagcagcagc ttttcggggc
    14581 ggcggcggat aatctggcgg cagagttccg aaccgatgga gccgcccgcg ccggttacca
    14641 tgacggtttt gccttcgatg tcggcactca tcaggcggtc gtcgggcgcg acggaatcac
    14701 gcccgagcag gtcggacaca gagatttttt tgagcgtgcc gatgctgatt tttccgtcca
    14761 tcaggtcttt cattccggga atggtcaaca cttcgcacgg ataggcttcc agtttgttga
    14821 tgattcggcg gcgttgttcc tgggtcgcgc cggggatggc gagcaggatt ttttccacgc
    14881 cgtagcgttc gatgaggaag gcgatggcat cgggctggta aacggcaagg tcgtagatga
    14941 cggtgtgcca cagtttgggg tcgtcgtcta caaaggcggc ggcggaatat tcgcgcattt
    15001 gtttgacggc ctcaagcagt tgtctgcccg accgtcccgc gccgtaaatg atgacaggga
    15061 tcatctgttt tttggggtgt tcggacaaca gtccgcgcaa aaccatacgc gagccggtca
    15121 cggaaacaaa cagcagtaag aaatagacaa tcggcagggc gaggcgcagc ctttcttcaa
    15181 aaatcaatgt gttgaggaag aacaggacgg cggaaacaat gctgcctgcc agcgcggtgg
    15241 agaggatgcg gaagctgacg aagcgtgtaa cggcgcggta aagccccatt cggataaata
    15301 atgtgatggt cagcaaggca gtcagcaaaa aagactgcca gttggcaaaa tcgaaccatt
    15361 cgtccgagta gtcggccttt aggctttggg tgaaccaaaa ggcaatgaaa atcatcagaa
    15421 aatcgtgtat gaggaaacag attttcttga tgttgcgcgg cagggcgatc agggtttcca
    15481 gagtcatgtt gtcggcggcg tgtatttttc agacggcata tgccgtctga atggttatcg
    15541 tgtggcttct gccaatacgg cttcgatgtg ttctttgcag aacgcgattt cgtcgtcggt
    15601 cagcgtcggg tgcaccaaga acatcaggct ggtgtcgccc agttcgacag catttttcaa
    15661 acgctctttc ggccgccaag gcgtgtcgtc gaaagatttt tccaaataga cttcggagca
    15721 gctgccttga tagcagggga ctttgcgcgc gttcagttcg ctgacgatgc ggtcgcgcgt
    15781 ccagccgtct ttgaggtgtt cgggtttgac gaaagcgtag aacttatatt gcgcgtgtcc
    15841 gatgtagtcg gcgacttcaa ccaagcggat acttttgaat ttgcctaggc tttccgccag
    15901 cttggcggcg tgcgcttgac ggcaggcagt ccattcgggc aggcgtttga gctggatgcg
    15961 cccgattgcc gcctgcattt ccatcatacg ccagtttgtg ccgaaacttt cgtgcagcca
    16021 gcggaaaccg ggcgcgtgtt cgcggtggta cacggcatcg tagcttttgc cgtggtcttt
    16081 gtacgaccac attttttccc acagggtttt gtcgttggtc gtaaccatgc cgccttcgcc
    16141 gcccgtggtc atgattttgt cttggcagaa cgaccatgcg ccgacgtgtc cgatgctgcc
    16201 gacggatttg cctttgtatt tcgcgccgtg cgcttgggcg cagtcttcga tgacccacaa
    16261 atcatgttct tttgccaagt ccatgatgcc gtccatttcg gcgggcatac cggcgaggtg
    16321 gacgacgatg atggctttgg tattcgacgt cagcgcggct ttgatagttt cggcgctgat
    16381 gttttggctg ttcaaatcca catcggcaaa cacgggattt gcgcccgcgt tcacaatgca
    16441 ggaagcggaa gccaagaaag tgcgcgaggt aacaatcaca tcgttgcccg cgcctatgcc
    16501 gattgctttg agcgcgacat cgagtgccag cgtgccgttg gaaagggcga cggcgtaccg
    16561 cgtgccggca aaggcggcaa attctttttc aaatttgcgg cattcgctgc ccgtccagta
    16621 gttgactttg ttggacagca ggactttgga aacggcatcg gcttcttctt gggtaaagca
    16681 gggccacggg gagagggagg tgttcagcat ggtgtttggt ccgtcgggtt cagacggcat
    16741 ttccgaccct atgccgtctg aaggggggcg tgttccgaag aatcgggcgc gcgccgcagg
    16801 tgttgtcaaa atcggtctgt acgggggtgt atagtggatt aaatttaaac cagtacggcg
    16861 ttgcctcgcc ttgccgtact atttgtactg tctgcggctt cgtcgccttg tcctgattta
    16921 aatttaatcc actatatttt aatcgcttat gccgtcccgg tcttggggtt tttgcgcggc
    16981 agcggctttg ccggattgcc cgcgacggtc atgccgtccg ggatgtcgca cacgataacc
    17041 gcccccgcgc ccattcaact tccatcgttt ccaaatggtg tcgaaaacgt agggcggaaa
    17101 gccgtttacg ccccatgaat aatattttgt catgaaatag tccttttaat attgtgagtg
    17161 catgtttgat tatgtaatgg ttaagtattt gaatcaatta taggaactaa atcttgaaag
    17221 gagttaaccc aaaagtcggg atgatattct gtgttaaaac tttcaggctg gtggcggtgg
    17281 atgttctgtc cggagggaag caatcctatg gttatccagc ccaaagtgtt cggcgcgata
    17341 aagtctttgg aaatgttgtc gccgatatag atgaagcagc ctgccttgtc ggcatatttg
    17401 tcttgaagat ggcggaagcg tttgctgtcg ggcttttccg aagagcaggc ttcagagatg
    17461 aggatgtcgt caaacaggga agacagaccc aaggcttcca gcttcaggcg ttgggtcagg
    17521 cttctgccgt cggtaatcag cgcgcgtgcg gcgaagggcg cggtcagttc ggacaggaaa
    17581 tccggcggag catacggagt cagcgtcggg cggtgcagcc ggtattgcca caaaagaacc
    17641 tgtttttccg attcattaaa accacagtgg cggcacaatt tgtccagcca atctttgccg
    17701 tcggggtcga tgttgcgcca taggttgtct gaattccaat cgggatacag tgcggcaacg
    17761 gtatcgatga ccgaacggat accggagagc ttgtattcgt attccgaata cagggtatcg
    17821 tccaagtcga agacgatgac ggtggtttca ggattaatcg ttttcatgaa ccaagacctt
    17881 ggcatcgtag cgcaacataa tgaggttgtt ttcccagtcg tctgaaaacg ggatttcttc
    17941 gtcgccgagg tattcgcgca tcaaccggct cggataattg ccgcctgcgg catacgtcag
    18001 cgggaagcca ccgccgaagc gggggttgat ttccacgccg tagaattcgc cggtttcttc
    18061 gttgtagaag aactgcgccg tgatacagcc gcgcgcgccc tgcaataccg ccattttttc
    18121 cagcagcgtt tggtagaggc tgtttttgca ggtaacgcct ttgctgattt cgccggagcg
    18181 cacttccaag cgttttcgcg ggatggcgca tttcagacgg cattgacggt cgtaatacat
    18241 atcgacggta aactcggaaa atttattttc gatgtcgata aactgacaga acattaattt
    18301 ggtgtctgcc aagacttccg gtgtcaggtc ggcaggtgtg tcaatccgtt ttgcaccgat
    18361 ggcgcggctg ccgtcataag gcttggcgaa gcaagggaaa accaacttct tgcggtcgta
    18421 aatttcgggt gagcgtatgc cgtagcgggc aaacaaggtg gcggttttcc gtttgtcgcg
    18481 gcattgtgcg atgaagtccg aatcggaaac gacgatgtgg acacttgccg cttcaaagcg
    18541 ttcgcgctcg tctgccagct tttgcagttc ggtgtctatg gttgggatga ttaaaccgat
    18601 attttcgcgt accgccagat tgaaaatgct gtcgatatat gaatcggcac taatcggcgg
    18661 cacttcaaat gaaccgtccg aaacatggca ggcagaagac atgcgggggt tgaggtcagt
    18721 ggcaaaaacg ctgatgccgt ctgaaaaacg tgcggcttcg gtttggaagt cctgcaccaa
    18781 ttcgacacgg cgacctgccg acagaatcag tatattattt ttttcattag tttgatttcc
    18841 agtgaaataa ggcatagtgg cttccccttc cgccgaaatc ccttctttga taaagacttt
    18901 tctgactgtc aaaaacagga ttttcatatc cagccaaaaa ctgtaatggt cgatatacca
    18961 aacatcgcag gcgaattttt cgtcccacga aagcgcgttg cgcccgttga cctgcgccca
    19021 gccggtaatg ccgggtttca tttcgtggcg gcggttttgg aagttgtcgt acagcggcag
    19081 atattgcatc agcagcgggc gcgggccgac caggctcatg tcgcctttta agacattcca
    19141 taattcaggc agttcgtcca aactagcggc acgcagtttt ttgccgaagg gtgtgagccg
    19201 ctcgctgtcg ggcagcggaa tgccgtctga atcaagcgcg tcgcgcatgg aacggaattt
    19261 gaccatttta aaaggttttc cgtcctttcc ggggcgttct tgaatgaaga agacgggcga
    19321 acccagattc ttgcggatga ggtacgccaa aatcaggaaa acgggtgaga ggacaatcag
    19381 ccccgatgcg gatgcgatga tgtcgaacag gcgtttgacg gctttactca tttgccaatc
    19441 tttcaatcag gttgacgatt ttccgatagg aaatgtcgcg cctgaagcgg cggacgattt
    19501 catcggactg aacggggtcg tttttgcgtt tcaaaatatc tttggcggct tgaacgaagc
    19561 tgtccacgtc gccggaacgg tagttttcgt gcggcagcag gttcaagagg ctaaggactt
    19621 cggcattggt ctggctgttc agaatcggtt tttgcaaagc catatagtcg gagagtttgt
    19681 tggtaaccga ctgcatggcg tggctatgga tggcgttgac ggcaatgtcg cagcctttgg
    19741 caatcgacat catttcagaa tagggtaaat aaccgtaaaa cttgatggcg cgattttcat
    19801 attgtttgag tttctccaaa tcgggtcctc cgcccataat gtgcaattcg acgttttcgc
    19861 cgtcgtctaa aagtttccga acacctttgc acacggtttc cacgtcgtag ctgtaactga
    19921 gcgttcccaa gtagaaaagg cgggtggttt tggagcggaa tcgcgggggg ggggcgatgg
    19981 cggcaaaatc cgtaccgata tagacggctt cgccgggtac gttcggattg gcttctttgg
    20041 cgcggtcgag ataagtctgc gatacggcaa ccagcgcgtc ggtaggcacg gttggcgcgt
    20101 gaagcaaagg gcagcagctt gtgcggtact tttttcaaaa atggtacgac cgaggagaaa
    20161 gactccggcc atacgtcctg cacatcgaca atcagtttgt agcccaaacg cgctttgtgt
    20221 ttgcccaaca gcaggttggt cgccatcagc ggataggcag aatagacgat gtcctgttcc
    20281 gccgcctgcg ggctgtgcag ccagcgtgcc aaattgcgga cgaatacgcg gtggctggcg
    20341 acgcgggcga gggagacgtt ttttcggtag ccaggttcgt ccagcagctt gacgcgcagc
    20401 ctgccgtttg aagtggcggc agcatcttca tgccgtctga aggatttgtc gtagtggcgg
    20461 aaacggctgg taatcagcaa cacgtcgtgc gattgcgaca atcgttctgc cagataccaa
    20521 aagcggttga aatacggttc ggacggcagc gagcagtagg gggcgactat ggtgatgttc
    20581 atcggcggct ttcagacggc attacatggt atacgccgcc atggtggtgc ggtagatttc
    20641 gtcgtcggaa cagagtgcgg cgacgtgtgc gtgcagcctt ttgcccattg cttcgcgcaa
    20701 ctccggttgt ctgacgagcg tgtctacggc ggcgataaag gcttcttcgt cgccgaaggg
    20761 gatgcagtaa cccgtttccc cgttgacgac catttcggaa atgcccgcca tatcgtaggt
    20821 aacgacgggc gtgtcgtaaa gtccggcttc taagatgttg ttgcctacac ctgcgccgtg
    20881 gtcgccgatg cagtggggcg tgttcaccaa aatatccacg tctttgaagt aggcggtcaa
    20941 atcgcgtatg ccgccgagga aggtaacttt gccgtctatg cccagacgtt ccgcctgcgc
    21001 tttcaaattg tccatttctt cgcctatgcc cgccacgttc aggcgtacgg gcatatcgcg
    21061 gtcgaccatt tttttgaaaa tgtccagcat cagatgcacg gcgcggacgg tgtccaggcg
    21121 cgacagcgtg ccgagcatga cgcagccttt gacggttttc cagaaaggaa a
//
LOCUS       DAWFLT010000027        20872 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWFLT010000027 DAWFLT010000000
VERSION     DAWFLT010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20872
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.027"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(25..716)
                     /locus_tag="WGR74_001041"
                     /pseudo
     CDS             complement(25..716)
                     /locus_tag="WGR74_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1022..1579)
                     /locus_tag="WGR74_001042"
     CDS             complement(1022..1579)
                     /locus_tag="WGR74_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905476.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGAATAAGIGAATGVA
                     AKQGWLLLPEADWLGIWHGDRVFMH"
     gene            complement(1823..1981)
                     /locus_tag="WGR74_001043"
     CDS             complement(1823..1981)
                     /locus_tag="WGR74_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905477.1"
                     /translation="MKTPLPICTLSALAACTLSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(1978..2820)
                     /locus_tag="WGR74_001044"
     CDS             complement(1978..2820)
                     /locus_tag="WGR74_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0905478.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPILVDSIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLTQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            2886..3029
                     /locus_tag="WGR74_001045"
     CDS             2886..3029
                     /locus_tag="WGR74_001045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905479.1"
                     /translation="MPSEKYGIRAVKLCGGKCAIIRAVLPLLRSCSANIRAAWTTYNR
                     IPA"
     gene            3059..3979
                     /gene="pyrB"
                     /locus_tag="WGR74_001046"
     CDS             3059..3979
                     /gene="pyrB"
                     /locus_tag="WGR74_001046"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ0905480.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLAHAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            3989..4447
                     /locus_tag="WGR74_001047"
     CDS             3989..4447
                     /locus_tag="WGR74_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0905481.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4512..5177
                     /locus_tag="WGR74_001048"
     CDS             4512..5177
                     /locus_tag="WGR74_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905482.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5240..6457)
                     /locus_tag="WGR74_001049"
     CDS             complement(5240..6457)
                     /locus_tag="WGR74_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0905483.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            6599..7144
                     /gene="def"
                     /locus_tag="WGR74_001050"
     CDS             6599..7144
                     /gene="def"
                     /locus_tag="WGR74_001050"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ0905484.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            7231..8157
                     /locus_tag="WGR74_001051"
     CDS             7231..8157
                     /locus_tag="WGR74_001051"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ0905485.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLRKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            8236..9495
                     /gene="rsmB"
                     /locus_tag="WGR74_001052"
     CDS             8236..9495
                     /gene="rsmB"
                     /locus_tag="WGR74_001052"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ0905486.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLREREKLASSCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNITAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            9461..10060
                     /locus_tag="WGR74_001053"
     CDS             9461..10060
                     /locus_tag="WGR74_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ0905487.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10060..12177
                     /locus_tag="WGR74_001054"
     CDS             10060..12177
                     /locus_tag="WGR74_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ0905488.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAVPVQIDLIGAASLPGDMGRVLEHY
                     ANGFTQLSLYNAASGKIEKSINPHKLDQPFPDKARWEKIQQAGSVRDLESIGGVLYAQ
                     GWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATLL
                     IASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTKL
                     FNHMTEQLSIAKEADERNRRREEAARHYLECVLDGLTTGVVVFDEQGCLKTFNKAAEQ
                     ILGMPLAPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKILL
                     GKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAERL
                     AWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALIG
                     DVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVKS
                     EAGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRIS
                     LSNQDAGGACVRIILPKTVETYA"
     gene            12170..13447
                     /locus_tag="WGR74_001055"
     CDS             12170..13447
                     /locus_tag="WGR74_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ0905489.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSTVENALKYGAAQTETGPVFDKLGNSAAIQEMNREVGAAVKRASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAERCRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPAAFSEDALAVLTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            13538..14725
                     /gene="dprA"
                     /locus_tag="WGR74_001056"
     CDS             13538..14725
                     /gene="dprA"
                     /locus_tag="WGR74_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ0905490.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRRFGSAQNALSAPAEQV
                     AALIRHKQALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVQLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGQNIPVVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            14751..15212
                     /locus_tag="WGR74_001057"
     CDS             14751..15212
                     /locus_tag="WGR74_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ0905491.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            15284..17590
                     /gene="topA"
                     /locus_tag="WGR74_001058"
     CDS             15284..17590
                     /gene="topA"
                     /locus_tag="WGR74_001058"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ0905492.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLSNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPSRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAESCPKCAWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(17663..18358)
                     /locus_tag="WGR74_001059"
     CDS             complement(17663..18358)
                     /locus_tag="WGR74_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905493.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFK"
     gene            complement(18378..18929)
                     /locus_tag="WGR74_001060"
     CDS             complement(18378..18929)
                     /locus_tag="WGR74_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905494.1"
                     /translation="MEFLSLHPWWSFFIILTIILSYIGFCAHLHIQNRAVFFYSYRDV
                     TIIFLTPVILIALSYLIKNKEILSACILCITLIAFSWAWIITYRSNTKRFFLSLLIVW
                     GKLLLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRN
                     LLNLCCLHKDFSTPSKNIEVNRS"
     gene            19048..19426
                     /locus_tag="WGR74_001061"
                     /pseudo
     CDS             19048..19426
                     /locus_tag="WGR74_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            19515..20084
                     /gene="rsmD"
                     /locus_tag="WGR74_001062"
     CDS             19515..20084
                     /gene="rsmD"
                     /locus_tag="WGR74_001062"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ0905495.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVCSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            20207..20458
                     /locus_tag="WGR74_001063"
     CDS             20207..20458
                     /locus_tag="WGR74_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ0905496.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEYPETHDELMAKYEKIIQFK"
     gene            20555..20638
                     /locus_tag="WGR74_001064"
     tRNA            20555..20638
                     /locus_tag="WGR74_001064"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20589..20591,aa:Tyr,seq:gta)
     gene            20669..20742
                     /locus_tag="WGR74_001065"
     tRNA            20669..20742
                     /locus_tag="WGR74_001065"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20701..20703,aa:Gly,seq:tcc)
     gene            20752..20826
                     /locus_tag="WGR74_001066"
     tRNA            20752..20826
                     /locus_tag="WGR74_001066"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20784..20786,aa:Thr,seq:ggt)
BASE COUNT         5242 a         5280 c         5679 g         4671 t
ORIGIN      
        1 aataaatggg caaaaatcgg tttattatcg tttttgccgc atttggattt gttctaccgt
       61 aaaacgtgtt tgacgaacgg gattcttatt aaaaaacatc tgatttctaa caaaatcagt
      121 attttttggc acgatggcta aaatttttcc ttccatttcg ccatcacgtg ttttccatgc
      181 gctcaagaat tgtgatttgc tcattgagac gtgccccagc gatggatcag ccagcaaaac
      241 agtttctccg ttaataccgt tcaataccga aaaatggttg tttttacggt attttaaata
      301 cacaattaca ggaattttta gttgtaccaa ctgttcaaat ggcaaagcat aaccttgtgc
      361 ttcaaaaccc agttcgggca ttatgcgttg catatcatca aaagaagcac gcatttgggt
      421 tttatccatt ttgtctaaga tttccgcttc agaataatgt ctgccataaa aattattcag
      481 taacgtggca atcgaagccg cgccgcaaga aaaatccaaa tcttgtttta ctatgccgga
      541 atctcgccgt gctttccaac tccgtacatg gatgttttgg taagaagcgg ggggtcaaca
      601 aacataggcc aagcaaaaac tatatttggg gcgaaaccaa tcaaagccgc ataatttatc
      661 aatttataaa gattttttat cataatatgt atacgcggaa taaacgcatg aaacataaaa
      721 aaataaaatc atatgcaatt ttattgcatg aataaatatg aataaaatag ataatgatgg
      781 gagtaaatac gccatgtatt ttggaagttt aaatttatta ataataaaat aatttatcgt
      841 agcgcaaata aatcccaaaa ttggaacgat tagaaaaaaa attaatacac ccatcatcat
      901 gcaaactcta tattaataaa tagctaacta attctattgt ggaaattagt tagctaacta
      961 aaagttatta atgattattt tcgagaattg actgcattgt tggcagcatt ggcaccaaaa
     1021 cctagtgcat gaatacccgg tctccatgcc aaattcccag ccaatccgcc tccggcagca
     1081 gcagccagcc ctgttttgcc gctactcctg ttgccgcacc gattcctgcc gcagtagccg
     1141 cgccttgcgc agttcctaat ttaccatgat tatacaaatt agcaccatga tactcccatg
     1201 cacctaatgc accgccaaaa gcagcggctg caataatggg aacaaattca ccttgtgttt
     1261 ctttcatttc agcctgtgat aattgaattg ctttcacatt ttggctgtca aaaacttggc
     1321 tgtctaaatt ttgcgccatt acaggtgtaa tcatcatagc cattacagtt gcaattttcg
     1381 ttgcgctggt ttgcacataa ataggattag caaattcgct ttgattgcgt tcagtgttga
     1441 tgtagctaat actgctttct agtttgaatt tacccttgtc agtcaataat tcttccaaac
     1501 ttaaaggtaa gtcagcataa gcaatttggc tggcaacagt taaaataaaa tctattagac
     1561 atttgtgttt ttgcatcatt tcgtttgatt ttctagtttt tgagaatgat acaaagtttt
     1621 ttacaaagta aagagtcact ctgaaaaaca ttttttcatt ataaatcaaa atattgatag
     1681 aataaatagc gagcatcgat tcacggtgca ctttagtgca aagcgtacaa cgcgagcctg
     1741 aaccaccagc cgcaacagga aaagaaagcc gatagagtgc aatgcttgcc aacgtgcaag
     1801 cgagcttgca agaacgcttg gctcaacgag agcaggcaag acagaaagca gaaagcagga
     1861 taggagcggt aacgcaaagg tctcgggctt tgatttcgcc gtaaaccctg ctgccgcctt
     1921 gtccggaaag ggtgcaggcg gcgagtgccg acagggtgca gatggggagg ggggttttca
     1981 tttggggtcg caacggaagt ggtatgcgca gatttcaaaa ccgtttttga aatacaggcg
     2041 gtgcgcgtcg gcacggtcgt ggttgacgtg gacgttgagg tggattttgg ttacccctgt
     2101 ttccgcgccg attttgcgga cttcttccaa aaggcgcgag gcgtagcctt tgcggcggct
     2161 ttgcggcagg gtaacgatgt catcgatgtg gatgtggcgg ccgctggcga gggtgcaggc
     2221 ttcgcggaag ccgcagacgg cgacggcatt gtgtttgcct tcttcaaaaa tacccagcag
     2281 gcggtagcct tgggggcgtt ggactttgtt gatctgttcg gtaaagcggt tgatgtcggt
     2341 cagggcggaa cgcaaaacgc tcaaggctgc aaaggcggtg gcggtgtcgt ccgcgccgat
     2401 ttcgcgcaaa acgtaggatg cgcccgaggc ggtctgttcc tgtgctttct cggcggcgtg
     2461 tttttcttcg attgcctgtg tcagcatgac gtgttcgtcg gcagggttgt tttgtccgcc
     2521 ctgttcgcgt tcttcgagca gggctttgca gtcgatgacg cgcaggtcgt tgtcggcggc
     2581 aaagtccatc aggaagcgga acatttgggg attgtcggtt tccagttttt tatcgacggc
     2641 gacgcagcgg atgctgtcca ccagtatggg gcgcacccat ttcgaatagg aaagcctgtg
     2701 gtctttggtg aacgaggaca gaatgccgca caggcgttcc gcccagtcgc tgggacggaa
     2761 aatcttgccg gaactcgttg tgccgtggat gacgacttcg taggggttgc agactaacat
     2821 ggcggcttcc tgaaaagaaa tgtctggctc gattatacct tatgcttatg caggcgtgtt
     2881 tggatatgcc gtctgaaaag tacgggattc gtgcggtaaa actttgcggc ggcaaatgtg
     2941 cgataatacg cgccgtattg ccgcttttgc gaagctgttc cgcaaacata cgggcggcgt
     3001 ggacgacgta taaccggata cccgcctgac gcgggttttt tacggaaggg gggcaaaaat
     3061 gcctaatccg ctttacagac agcatatcat ctccatttcg gatttgtcgc gcgaacagtt
     3121 ggaatgcctg cttcagacgg cattgaagct gaaggcgcat ccgcgcggcg atttgttgga
     3181 aggcaaactt atcggttcgt gcttctttga accgtccacg cgcacgaggc tgtcgtttga
     3241 aacggcggtg cagcggctgg gcggcaaggt catcggcttt tcggacggcg cgaataccag
     3301 tgccaaaaaa ggcgagacgc ttgccgatac cgcccgcatc atttccggct acaccgacgc
     3361 aatcatccaa cgccacccca aagacggcgc ggcgcgcgtg gcagcggagt tttcgcgcgt
     3421 ccccgtcatc aacgccggcg acggcaccaa ccagcacccc agccagacct tgttggatct
     3481 ggtaacgatt tatgaaacgc aggggcgttt ggacaagctc aaaatcgcca tggcgggcga
     3541 cttgaaatac gggcgcaccg tgcattcgct ttgtcaggcg ttgaaacgct ggggctgcga
     3601 atttgccttt gtttcgccgc ccagcctcgc gatgcccgac tacattaccg aagagttgga
     3661 cgaagccggc tgccgatacc gtatcctcgg cagtttggaa gaggcggcgg aatgggcgga
     3721 catcctgtat atgacccgcg tccagcgcga acgtttcgat gaacaggaat ttgccaaaat
     3781 ccaaggcaaa ttcaacctcg aagcgtctat gctcgcccac gccaaaccga acctgcgcgt
     3841 gctgcaccct ttgccgcgcg tggacgaaat ccatcccgat gtcgatgcca cgccgcatgc
     3901 ctattatttc gagcaggcaa ccaacggcgt ttatgcgcgt atggcgatat tgtcgctggt
     3961 gttgaacgaa gaagtgtgag gaaccgatat ggaaaccccg aaactcagtg tcgaagccat
     4021 tgaaaaaggt acggttatcg accatattcc cgccggcagg gggctgacca tcctgcgcca
     4081 gttcaaactt ttgcactacg gcaacgcagt aaccgtgggc ttcaacctgc ccagcaaaac
     4141 ccaaggcagc aaagacatca tcaaaatcaa aggcgtgtgc ttggacgaca aagccgccga
     4201 ccgccttgcc ctgtttgcac ccgaagcagt ggtcaacacc atcgacaatt tcaaggttgt
     4261 gcagaaacgg catttgaacc tgcccgacga aatcgccgaa gtgttccgct gtccgaacac
     4321 gaattgcgcc ggccacggcg agccggtcaa aagccggttt tatgttaaaa aacacaacgg
     4381 gcagacgcgg ctgaaatgcc attactgcga aaaaacctac agccgggatt cggtggcgga
     4441 agcctgacgg attcccttaa accgagcggg cggcatttcg tctgccgctt gttttgccaa
     4501 tctgaaatgg aatgatgatg cacgcttctg tccaaagccg tttcgcaccg atactttatg
     4561 ttttgatttt ctttgccggt tttttgaccg cgcaaatctg gttcaatcag aaagcctata
     4621 ctgaagagct gcctccgctt ctgtccgcat tgtccgccgt cgcgctggtg tggctggcgt
     4681 gggcgttcgt gtcggcgcgt tcaaaggcta aggcggaaaa gttctaccgc gaaaaaatga
     4741 tacagaacga aagcatacac cccgtcctgc acgcttcttt gcaacacttg gaacacaagc
     4801 cgcaaatgct cgccctgctg gtcaaaaacc acggcaaagg tatggcggag caggtcaggt
     4861 tcaaggcaga agtgctgccc gacgacgaag acgcgcgcac gattgccgcc gagttggcaa
     4921 aaatggatat gttcgcattg gggacggacg cggtcgcctc gggcgaaacc tatggacgcg
     4981 tgttcgccga tattttcgag ttgtcggcgg ctttggaagg gcgcgcgttc aaaggaatgt
     5041 tgaaactgac ggcggaatat aaaaacatct tcggcgatgc ctgccgttcg gaaacggcgt
     5101 tggagttggg cgcgctcaat caggcgttgc aggagatttc aaaaacatcg gaaaagtcca
     5161 aacggatatt ttattgaaga tggaaaaatg ccgtctgaaa cggaaggtgt ttcagacggc
     5221 atttttgtcg gatgattaat tattcggagc ggttgaagcc aaacttcacg cggctgcggc
     5281 cctgatccgg tatattgtcc aaatcgcgtc ccggattggc ggcggtgtcg cctacggaaa
     5341 tatcggagat gttttccaaa atgatggcgg acgacaggtg ttcggaggtg cggtaaacca
     5401 ttgccaagcc cacttcttcg gcaggagtgg aaatcagctc gacggtatcc ctgcttttga
     5461 aattgttgga gaggtcgacc tgcatcgttt tcttgcgttt gtagaggctc aaaaccgtgc
     5521 ctttgtccaa accgtccgcc tcgcctttgt cgatggtgat ggtttgaaac tggccggcaa
     5581 tccttgtgcc ttcaaacacg gaaacgattt tagcctgaac cgggcgggac ggttcgtgcg
     5641 gcatcatgtt gaagcggtcg gtgtcttccg gcattttcat caggtagtcg ccctgctgta
     5701 tttcggaaat ggcggtttcg accaccagcg gctgtatcga aggggtgcgc agcggggtaa
     5761 tcaaaggatg ggtgcgggta tggtattcgt tgtctttcgg ccgttctcca gcctgtttcg
     5821 agcgttgttc gaggacagag tcggtatagt cgagggagcg cacgatgccg ctgaatgcga
     5881 cttcctgccc gaggaattta cccgtatccg gatcggtgat gtttttattg attcggtagg
     5941 tcaggtagcg acccggctct ttcaggcctt tggtgtaaac cctggtgcct ttggtgtaca
     6001 gcagcctgcc ttccgggccc gagagcaggc gcggcgcggc agcggtttct ttgcgggaaa
     6061 cgatttgcgg gtgccgcata aagatgcggt agaagttgac atcgatggcg ggaataccgt
     6121 atccggacac ttccttatcc ggactcattt tgacgacggg gatgccgtct gtctgttcca
     6181 agccgaggcg cggttcgccg tcaacgtggc gcaacaccaa tacctggtcc ggataaatca
     6241 ggtcgggatt gtggatttga tcccggttcg cgtcccacag gcggccccat tgccacgggc
     6301 tgtacaggta tttgcccgaa atgccccaca gggtgtcgcc ctgtttgacc gtgtagcgtt
     6361 ccggcgcgtt cgggcgcacc tccaaatttg ccgccaaagt ttgtgttgag aatgccatac
     6421 ctgccgcgca gagcagggtt ataatacgac gttgcataac cgttcccctt atctgataaa
     6481 tttcggtttg tcttgcttga ttgggttgga aaaagcggcg gcagcccctc gggatgtgcc
     6541 gcgtgataaa aaatgttccg cattttaaca tcgaattatc cgcaccatca cggtaattat
     6601 gaaaaacagg cggcgtatcc gccgaaggaa agagaaaatt atggctttat tgaatatctt
     6661 gcaatatccc gacgagcgtc tgcacacggt ggcaaagcct gtcgaacaag tcgacgagcg
     6721 catccggaag ctgattgccg atatgtttga aacgatgtac gaatcgcgcg gcatcgggct
     6781 ggcggcgacg caggtcgatg tgcacgagcg cgtggtcgtg atggatttga ccgaagaccg
     6841 cagcgaaccg cgcgtgttca tcaaccccgt catcgttgaa aaagacggcg aaaccactta
     6901 cgaagagggc tgcctgtccg tgccgggcat ttacgacacc gtaacccgcg ccgaacgcgt
     6961 caaggtcgag gctttgaacg aaaaaggcga aaagttcacg ctggaggcgg acggcttgct
     7021 ggcgatttgc gtgcagcacg agttggacca cctgatgggc atcgtgtttg tcgaacgcct
     7081 gtcgcagctc aagcagggac gcatcaaaac caagctgaaa aaacgtcaga aacatacgat
     7141 ttgacccttt tgccgtgccg tctgaacgct gcaaagtttt cagacggcac ggtcttgtcc
     7201 gacaacctta cgcatacgca ggaatatgct atgaaagtca tctttgccgg cacgcccgat
     7261 tttgccgccg ccgccttaaa agccgttgcc gccgccggtt ttgaaatccc gctggtgctg
     7321 acccagcccg accgtccgaa agggcgcggt atgcaactga ccgccccgcc cgtcaaacaa
     7381 gccgcgctgg aactcggttt gcgcgtggca cagcccgaaa aactgcgtaa caacgccgaa
     7441 gccctgcaaa tgctcaaaga ggtcgaggca gacgtaatgg tggttgccgc ctacggtttg
     7501 attctgccgc aggaagtgtt ggatacgccg aaacacggct gcctcaacat ccacgcttcg
     7561 ctgttacccc gttggcgtgg cgcggcgccg attcaacgcg cgattgaagc cggcgatgcc
     7621 gagacaggcg tgtgtattat gcagatggac atcggtttgg acaccggcga tgtggtcagc
     7681 gaacaccgct acgccatcca accgaccgat accgccaacg aagtccacga cgcgctgatg
     7741 gaaatcggtg cggcggcggt tgttgccgat ttgcaacagc ttcaaagcaa aggccgtctg
     7801 aacgcggtca aacagcccga agaaggtgtt acttacgccc aaaaattgcg caaagaagag
     7861 gcgcgtatcg attggagcga aagcgcggcg gttatcgaac gcaaaatccg cgccttcaac
     7921 cccgtgcctg ccgcgtgggt tgagtatcag ggcaagccga tgaaaatccg gcgggcggaa
     7981 gtggtggcgc aacaaggcgc ggcaggcgaa gtgttgtcct gttcggcgga cggtttggtc
     8041 gttgcctgcg gcgaaaacgc gctgaagatt accgaattgc agcctgccgg cggcaggcgt
     8101 atgaatatcg cggcgtttgc agcaggacgg catatcgaag cagggacgaa gctgtaaacc
     8161 tttttcagac ggcatttgat ccgcaaacgg gaatgccgcc tgaaaccatc agttgaagaa
     8221 agcgaatcac ataatatgag tatggcactt gcccaaaaac ttgccgccga cagcattgcg
     8281 gcggttgccg aaggacgcaa ccttcaggac gtgttggcgc aaatccgcac cgcgcatccc
     8341 gaccttacgg cgcaggaaaa cggcgcgttg caggacatcg cctacggctg ccagcgttat
     8401 ttgggcagtt tgaaacatat gctcgcgcag atgctgaaaa agccgattga caacccgcag
     8461 ctcgaaagcc tgcttttggc ggcgttgtac caactgcatt acacgcgcaa cgcgccgcac
     8521 gccgtggtca atgaggcggt ggaaagcatc gcgaaaatcg ggcgcgggca gtaccgttcg
     8581 tttgccaacg cggttttgcg ccgttttttg cgtgaacgtg aaaaattggc gtcttcctgc
     8641 aaaaaagacg atgtggcaaa acacaacctg ccgctgtggt gggtggctta cttgaaaaac
     8701 cattatccga aacactggca caacatcacc gccgcgctgc aatcgcatcc gccgatgacg
     8761 ttgcgcgtca accgccgaca cggcaatgcc gaaagctatt tggaaaaact ggctgcggaa
     8821 ggtatcgccg caaaggcgtt ggacgaatat gcggttacgt tggaagaagc cgtgccggta
     8881 aaccgtctgc ccggtttttc agacggcatt gtttcggtac aggacttcgg tgcgcagcag
     8941 gcggcgtatc tccttaaccc gaaagacgga gaacggattt tggacgcgtg cgccgcgccg
     9001 ggcggcaaga cggggcatat tttggaactg gcggattgcc gcgttaccgc cttggacatc
     9061 gatgcaggcc gtctgaagcg tgtggaggac aatatcgccc gtttgggctt tcagacggca
     9121 tcggcggcgt gtgccgatgc gcgggatttg gcggcgtggt atgatggaaa gcctttcgac
     9181 gcaatccttg ccgacgtgcc gtgcaccgct tccggcgtgg cgcggcgcaa tcccgacgtg
     9241 aaatggctgc gccgtccgac cgacgcgcta aaaaccgccc gccagcagga agccctgctg
     9301 gacgcacttt ggcagacgct gaaacagggc ggcagaatgc tgcttgccac ctgttccgtg
     9361 ttcgtcgaag aaaacgacgg gcaattgcaa aaattcctca accgccatgc cgatgccgaa
     9421 ccgatcgaat cgcgggtgct tttaccgaac aaacaccaag atggctttta ttacgcgctt
     9481 attcaaaagc attaaacaat ggcttgtgct gctgccgatg ctttccgttt tgccggacgc
     9541 ggcggcggag gggatagatg tgagccgcgc cgaagcgagg ataaccgacg gcgggcagct
     9601 ttccatcagc agccgcttcc aaaccgagct gcccgaccag ctccaacagg cgttgcgccg
     9661 gggcgtgccg ctcaacttta ccttaagctg gcagctttcc gccccgataa tcgcttctta
     9721 tcggtttaaa ttggggcaac tgattggcga tgacgacaat attgactaca aactgagttt
     9781 ccatccgctg accaaccgct accgcgttac cgtcggcgcg ttttcgacag actacgacac
     9841 cttggatgcg gcattgcgcg cgaccggcgc ggttgccaac tggaaagtcc tgaacaaagg
     9901 cgcgctgtct ggtgcggaag caggggaaac caaggcggaa atccgcctga cgctgtccac
     9961 ttcaaaactg cccaagcctt ttcaaatcaa tgcattgact tctcaaaact ggcatttgga
    10021 ttcgggttgg aaacctctaa acatcatcgg gaacaaataa tgcgccgttt tctaccgatc
    10081 gcagccatat gcgccgtcgt cctgttgtac ggactgacgg cggcaaccgg cagcaccagt
    10141 tcgctggcgg attatttctg gtggattgtt gcgttcagcg caatgctgct gctggtgttg
    10201 tccgccgttt tggcacgtta tgtcatattg ctgttgaaag acaggcgcga cggcgtattc
    10261 ggttcgcaga ttgccaaacg cctttctggg atgtttacgc tggttgccgt actgcccggc
    10321 gtgtttctgt tcggcgtttc cgcacagttt atcaacggca cgattaattc gtggttcggc
    10381 aacgataccc acgaggcgct tgaacgcagc ctcaatttga gcaagtccgc attgaatttg
    10441 gcggcagaca acgccctcgg caacgccgtc cccgtgcaga tagacctcat cggcgcggct
    10501 tccctgcccg gggatatggg cagggtgctg gaacattacg ccaacgggtt tacacagttg
    10561 tccctttaca atgccgcaag cggcaaaatc gaaaaaagca tcaacccgca caagctcgat
    10621 cagccgtttc cggataaggc gcgttgggaa aaaatccaac aggcgggttc ggtcagggat
    10681 ttggaaagca taggcggcgt attgtacgcg cagggctggc tgtcggcggg tacgcacaac
    10741 gggcgcgatt acgccttgtt tttccgtcag ccggttccca aaggcgtggc agaggatgcc
    10801 gtcttaatcg aaaaggcaag ggcgaaatat gctgagttga gttacagcaa aaaaggtttg
    10861 cagacctttt tcctggcaac cctgctgatt gcctcgctgc tgtcgatttt tcttgcactg
    10921 gtgatggcgc tgtatttcgc ccgccgtttc gtcgaacccg tcctatcgct tgccgagggg
    10981 gcgaaggcgg tggcgcaagg cgatttcagc cagacgcgcc ccgtgttgcg caacgacgag
    11041 ttcggacgct tgaccaagtt gttcaaccac atgaccgagc agctttccat cgccaaagaa
    11101 gcagacgagc gcaaccgccg gcgcgaggaa gccgccaggc attatcttga atgcgtgttg
    11161 gatgggctga ccacgggcgt ggtggtgttt gacgaacaag gctgtctgaa aaccttcaac
    11221 aaagcggcgg aacagatttt ggggatgccg ctcgcccccc tgtggggcag cagccggcac
    11281 ggttggcacg gcgtttcggc gcagcagtcc ctgcttgccg aagtgtttgc cgccatcggc
    11341 gcggcggcag gtacggacaa accggtccat gtgaaatatg ccgcgccgga cgatgccaaa
    11401 atcctgctgg gcaaggcaac cgtcctgccc gaagacaacg gcaacggcgt ggtgatggtg
    11461 attgacgaca tcaccgtgct gatacacgcg caaaaagaag ccgcgtgggg cgaagtggcg
    11521 aagcggctgg cacacgaaat ccgcaatccg ctcacgccca tccagctttc cgccgaacgg
    11581 ctggcgtgga aattgggcgg gaagctggat gagcaggatg cgcaaatcct gacgcgttcg
    11641 accgacacca tcgtcaaaca ggtggcggca ttgaaggaaa tggtcgaagc attccgcaat
    11701 tatgcgcgtt ccccttcgct caaattggaa aatcaggatt tgaacgcctt aatcggcgat
    11761 gtgttggcat tgtatgaagc cggtccgtgc cggtttgcgg cggagcttgc cggcgaaccg
    11821 ctgatggtgg cggcggatac gaccgccatg cggcaggtgc tgcacaatat tttcaaaaat
    11881 gccgccgaag cggcggaaga agccgatgta cccgaagtca gggtaaaatc ggaagcgggg
    11941 caggacggtc ggattgtcct gacggtttgc gacaacggca aagggttcgg cagggaaatg
    12001 ctgcacaacg ccttcgagcc gtatgtaacg gacaaaccgg cgggaacggg attgggtctg
    12061 cctgtggtga aaaaaatcat tgaagaacac ggcggccgca tcagtttgag caatcaggat
    12121 gcgggtggcg cgtgtgtcag aatcatcttg ccaaaaacgg tagaaactta tgcgtagcag
    12181 cgatatttta attgtagacg acgaaatcgg catccgcgac ctgctgtcgg aaatcctgca
    12241 ggacgaaggt tattcggtcg cattggcgga aaacgccgaa gaggcgcgca agctgcgcca
    12301 tcaggcgcgc cccgcgatgg tgctgctgga tatttggatg cctgattgcg acggcatcac
    12361 ccttttgaag gagtgggcga aaaacgggca gctcaatatg ccggtggtga tgatgagcgg
    12421 gcatgccagc atcgataccg ccgtggaagc caccaaaatc ggtgcgctcg actttttgga
    12481 aaaaccgatt tccctgcaaa agctgctgtc cacggttgaa aacgcgttga agtacggtgc
    12541 ggcgcaaacc gaaacggggc ctgtattcga caagctgggc aacagtgcgg cgattcagga
    12601 aatgaaccgt gaggtagggg ctgcggtgaa acgtgcctct cccgtacttt tgacgggcga
    12661 ggcaggttcg ccgtttgaaa cggtggcacg ctatttccat aaaaacggta cgccgtgggt
    12721 cagcccggca agggtcgaat atctgatcga tatgccgatg gaactgttgc agaaggcgga
    12781 gggcggcgtt ttgtatgtcg gcgacatagc ccaatacagc cgcaacatcc aagccggcat
    12841 tgcctttatt gtcggcaagg cagaacgctg ccgcgtcagg gtggtcgcat cgggcagcag
    12901 ggcggcaggt tcagacggca ttgcctgcga ggaaaagctg gcggaactgc tgtcggaatc
    12961 ggtcgtccgt attccgccgc tgcgtatgca gcatgaagat attcccttcc tgatacaggg
    13021 gattgcctgc aatgtggcgg aaagccaaaa gattgcgccc gccgcattca gtgaagatgc
    13081 gcttgccgtg ctgacccgtt acgactggcc gggaaatttc gaccaactgc aaagcgtcgt
    13141 tgcaacgctg ttgttggagg cggacggaca ggaaatcggc gcggcggcgg tttcttccct
    13201 tttggggcag aatgtgcctg ccgagggagc agaagatatg gtgggcgggt ttaatttcaa
    13261 cctgcccctg cgcgaattga gggaggaggt ggagcggcgt tatttcgagt accacatcgc
    13321 ccaagaaggt cagaatatga gcaaagtggc gcagaaagtc ggtttggaac gcacgcacct
    13381 ttaccgcaaa ctcaaacagc tcggcatcgg cgtttcgcgc cgggcggggg aaaaaaccga
    13441 agaataggcc cggacggccg gttttccggc tgcgggcttt tgttttcaga cggcatttgg
    13501 tgcaaatgcc gtctgaaatc gtaaggggac ggattttatg acagaggacg aacgtttcgc
    13561 gtggctgcaa ttggcgttta cgccctatat cggcgcggaa agtttcctgc tgctgatgcg
    13621 ccgtttcggc agcgcgcaaa atgccctgtc cgcaccggcg gaacaggtgg cggcactgat
    13681 acggcacaaa caggcgcttg aggcttggcg caatgcggaa aaacgcgctc tggcgcggca
    13741 ggcggcagaa gcggcattgg aatgggaaat gcgggacgga tgccgtctga tgctgcttca
    13801 ggatgaagat tttcccgaaa tgctgacgca ggggctgacc gcgccaccgg ttttgttttt
    13861 gcgcggcaac gtacaactgc tgcacaaacc ttccgccgcc attgtcggca gccgtcatgc
    13921 cacgccgcag gcaatgcgga ttgccaaaga tttcggcaag tcgttgggcg ggcaaaatat
    13981 ccccgttgtg tcgggtatgg cttccggtat cgatactgcc gcccatcagg gcgcactgga
    14041 ggcagaaggc ggcaccatcg ccgtgtgggg gacgggtata gaccgcattt acccgccgtc
    14101 caataaaaac cttgcctatg aaatcgccga aaaaggattg attgtcagcg agtttcccat
    14161 cggtacgcgc ccgtatgccg gcaattttcc gcgccgcaac cgcctgattg ctgccctgtc
    14221 gcaagtaacg ctggtggttg aagccgcctt ggaatccggt tcgctgatta ccgccggctt
    14281 ggcggcagaa atggggcgcg aagtgatggc ggtacccggt tcgatagaca atccgcacag
    14341 caaaggctgc cacaaactga ttaaagacgg cgcaaaattg gtggaatgcc tggacgacat
    14401 cctgaacgaa tgcccggggc tattgcaaaa tacgggtgct tcatcatatt ctataaataa
    14461 ggatacgccc gacacgggcc gccgcgccgt tcagacggca tacgccccgc cgcctgccgc
    14521 aaaaatgccg tctgaagggg cggcaggcgg cacagccgtc ggcggcatac tggataaaat
    14581 gggtttcgac cccatccatc ccgacgtgct tgccggacag ttggctatgc ctgccgcaga
    14641 tttgtatgcc gcactgttgg aattggaatt ggacggcagc gttgccgcaa tgcccggcgg
    14701 cagataccag cgtatccgaa cttgaacgca ctttatatta aggaacacga atgaccgaag
    14761 tcatcgccta cctcatcgaa catttccaag atttcgatac ctgtccgccg cccgaagact
    14821 tgggtatgct gcttgaagaa gcgggtttcg atacgatgga aatcggcaac accctgatga
    14881 tgatggaagt attgctcaac agctccgaat tttccgccga acctgccgac agcggcgcat
    14941 tgcgcgtgta cagcaaagaa gaaaccgaca acctgccgca ggaagtgatg gggctgatgc
    15001 agtatctgat tgaagaaaaa gccgtcagct gcgaacagcg ggaaatcatc atccacgcgc
    15061 tgatgcacat ccccggcgac gaaattaccg tagataccgc caaagtgctg accctgctgc
    15121 ttttatgggc aaacaagagc gagctgcccg tgttggtcgg cgacgagctg atgagcgcgc
    15181 ttttactcga caacaaaccc acgatgaact gaagcggctt cagacggccc gcccgaggcc
    15241 gtctgaaacg ccggcatcaa aaccaccatc cagagaacga caaatggcga aaaacctatt
    15301 aatcgtcgaa tccccgtcca aagccaaaac cctgaaaaaa tatttgggcg gcgattttga
    15361 aatccttgca tcctacggac acgtccgcga cctcgtcccc aaaagcggcg cggtcgatcc
    15421 cgacaacggc tttgcgatga aataccaact catcagccgc aacggcaaac acgtcgatgc
    15481 catcgtcgcc ggtgccaaag aagctgaaaa catctacctc gccaccgacc cggataggga
    15541 aggcgaagcc atttcctggc atcttttgga aatcctcaaa tccaaacgcg gcttgaaaaa
    15601 catcaagccg cagcgtgtcg tgttccacga aatcaccaaa aacgccgtgc tcgatgccgt
    15661 tgcccatccg cgcgaaatcg aaatggactt ggtcgatgca caacaagccc gccgtgcttt
    15721 ggactatttg gtcggcttta acctttcgcc attgttatgg aaaaaaatcc gccgaggttt
    15781 gagcgcgggg cgtgtgcaaa gccctgcttt gcgcctgatt tgtgagcgcg aaaacgaaat
    15841 ccgcgcgttt gaagcgcagg aatattggac ggtgcatctc gacagccaca aaggccgcag
    15901 caagttcacc gccaaactcg cccaatacaa cggcgcgaaa ctcgaacaat tcgacctgtc
    15961 gaacgaagcc gcgcaagccg acgtattgaa agaactcgaa ggcaaagaag ccgtcgttac
    16021 cgccattgaa aagaaaaagc gcagccgcaa tcctgccgcg ccgttcacca cttctaccat
    16081 gcagcaggat gccgtgcgca aactcggctt caccaccgac cgcaccatgc gtaccgccca
    16141 acagctttat gaaggtatag acgtagggca gggcgcgatc ggtctgatta cctatatgcg
    16201 taccgacagc gtgaatttgg cggatgaagc gttaaccgaa atccgccatt acattgaaaa
    16261 caaaatcggc aaagaatatc tgccgagtgc cgccaaacaa tacaaaacca aatccaaaaa
    16321 cgcccaagaa gcccacgaag ccatccgtcc gacttccgtg taccgcacgc ccgaaagcgt
    16381 caaacccttc ctgagcgcag accagttcaa actctatcaa atgatttggc agcgtaccgt
    16441 cgcctgtcag atgacgcctg ccaaattcga ccaaaccacc gtcgatatta ccgtcggcaa
    16501 aggcgtattc cgcgtaaccg gacaagtgca aaccttcgca ggcttcttga gcgtttatga
    16561 agaaagcagc gacgatgaag aaggcgaaga cagcaaaaaa ctgcccgaaa tgagcgaagg
    16621 cgataaattg cccgtggaca aactctacgg cgaacaacac tttaccactc cgccgtcgcg
    16681 ctacaacgaa gccacgctgg ttaaagccct cgaagaatac ggcatcggcc gcccctcgac
    16741 ctacgccagc atcatctcca cgctcaaaga ccgcgaatac gttacccttg agcaaaaacg
    16801 cttcatgccc accgacacag gcgacatcgt caacaaattc ctgaccgaac acttcgccca
    16861 atacgtcgat taccacttca ccgccaaact cgaagaccag cttgacgaaa ttgccgacgg
    16921 caaacgccaa tggattccct tgatggacaa attctggaaa ccgttcatca aacaagtgga
    16981 agaaaaagaa ggcatcgaac gcgccaaatt taccacgcag gaacttgatg aaacctgccc
    17041 gaaatgcggc gaacacaaac tgcaaatcaa attcggcaaa atgggtcgtt ttgttgcgtg
    17101 tgccggttat cccgagtgca gctacacgcg caatgtcaac gaaaccgccg aagaggccgc
    17161 cgagcgcatc gccaaagccg aagccgaaca ggccgaactc gacggacgcg aatgtcctaa
    17221 atgtggtggt cgcctagtgt acaaatacag ccgaaccggc agcaaattca tcggctgcgc
    17281 caactatccg aaatgcaaac acgtcgagcc gctggaaaaa ccgaaagata ccggcgtcca
    17341 gtgtccgcaa tgcaaaaaag gcaacctcgt cgagcgcaaa tcccgctacg gcaaactgtt
    17401 ttacagttgc agcacctatc ccgactgtaa ctacgccaca tggaacccac ccgttgcaga
    17461 aagctgtcca aaatgcgcat ggccggtttt aaccatcaaa accaccaaac gctggggcgt
    17521 cgaaaaagtc tgcccgcaaa aagaatgcgg ctggaaagaa cagattgaac cgcctgcgcc
    17581 gaaagagtga gattaggttg gtttgaaaga gaaaaggtcg tctgaaaaat tttcagacga
    17641 cctttgcttt tctgtgattg gtttatttga atccgcgtgt tgttttaaag tccgataaaa
    17701 tccggttcat ttcaggcgca aacaaggcga tgtaatcgta agatagaccg cgactggcac
    17761 tgggatgggg aaagcagacg acttcgcaat cttcaaacga ttggaatttg acattgaaac
    17821 gtgtaccgtc aaattctttt tgcaccgtct ccagcggttt ggtctgctta ccgaccaact
    17881 gctcgaagcg tggcagtaca ttttggttgt tcagaaaatc cgccaacctg ctgcccatga
    17941 agaggatgac tttcggacgc agtttttcga tgtggtagag aaaattatcg atgtgctcgg
    18001 gttgtgtgaa cttgtcggga ttgtcgatag tgttgccctg tgtagcagcc cagttggttt
    18061 gaaccaggga tttttcaaat gcaccgccca atccattttc gtctaagggg tgtccccaca
    18121 tttcaaacca attttttatc gtattgtcgt aacgccactt ttttgcctgc tctccgaaat
    18181 agagggattt gtttgcaaat gtatggtcga ttttgttttc agggagtttg tattcacctg
    18241 ctacataagc agcctcatcg gctttactcc aaccccattc atagccacaa atcattaagc
    18301 catgtttgtc gttgtagcct ttgaacaggc tgttgctcaa attcaaatcc ttcatcatga
    18361 actcttcctt ttaaaattta agagcgattg acttcaatgt ttttagatgg ggtggaaaaa
    18421 tccttgtgta ggcaacataa attcaataaa tttcttgata attcgaaacc tactaatagc
    18481 gcacctataa aagctttttc attacgttca gcatgacggt cacgtcgttc atatttttta
    18541 cgcttgctgt tccctgttat tacagctaag ccaagtgata tggcgagaat tgcccaaaca
    18601 atagtactta ataacaattt tccccatact atcaataagg aaagaaaaaa ccttttagta
    18661 ttagatcgat aggttataat ccatgcccat gaaaatgcaa taagagttat acataagata
    18721 caagctgaca ggatttcttt atttttaatt aaataactta gagcgatgag gatgacaggt
    18781 gtcaagaaaa taatagttac atcccgatag ctataaaaga aaactgccct attttgaatg
    18841 tggagatgtg cacagaatcc aatatagcta aggataatag ttaatataat aaaaaaagac
    18901 caccaagggt gaagagatag gaattccatg ttttcccttt ttttgtaaaa aggaaaaaat
    18961 cttatctaat agttaattgc ctaaccagcc agaagaagtt taaaatctat cccaataatt
    19021 caaccatcta tacagaaagt tcagcttatg gaaacccacg aaaaaatccg cctgatgcgc
    19081 gaattgaata aatggtccca ggaggatatg gcggaaaagc tggcgatgtc ggcaggaggg
    19141 tatgccaaaa tcgaacgggg cgaaacgcag ttgaatatcc cgcgtttgga gcagttggct
    19201 cagattttca aaatcgatat gtgggacttg ctcaaatcgg gcggtggtgg gatggtgttt
    19261 cagattaatg aaggtgatag tggtggcgat atttgcgttg tatgcgtcgg gtgatgtttc
    19321 gatgaaaata gaatttttaa aaatggagtt gaaacactgc aaagaaatgt tggaacaaaa
    19381 agacaaagaa atcgagctgc tccgcaagct gaccgaaacc gtttaaacag atatgccgtc
    19441 tgaaaaaagt tttcagacgg catattcttt gacaggtctt gtataatacc gtttgaactt
    19501 acaggttttt gattatggcg gcaggcaaac ataccaaaca cagcaaccgg gtacgcatta
    19561 tcggcgggca atgccggggc aggaaattga gtttcacatc cgccgacgga ctgcgtccga
    19621 cacccgacag cgtgcgtgaa aagctgttta actggctggg acaggatttg acgggtaaaa
    19681 cggttttgga tctcttcgga ggcagcggcg cactcggtat agaagccgct tcgcgcaacg
    19741 ccaaacgcgt gctgatttcg gataacaacc gccaaaccgt gcagaccttg cagaaaaaca
    19801 gtcgtgaact gggtttgggg caggtgcaaa tcgtctgttc agacggcatc gcatatttga
    19861 agaccgtatc cgaacagttt gatgttgtct ttctcgaccc gccgtttgca tggcaggact
    19921 ggcaaatcct gtttgatgcc ttgaagccgt gcctgaaccc ccgggcattc gtctatctcg
    19981 aggcgggtac gctgccggat attcccgatt ggctgacgga atatagagag gggaaatcgg
    20041 ggcagagtac atttgaatta agggttttcc aagtggctga ataatatgcg ctttgataat
    20101 catttccgag ttgtaaacat tcgtttgcaa ccgtccggtt caaaaaaacc ttgtgctata
    20161 atccgcgccc gcccggtttt gataatttag tggaaaagga aaagaaatgt cgctttttat
    20221 taccgacgag tgcatcaact gcgacgtatg cgaacccgaa tgccccaatg atgccatttc
    20281 ccaaggcgag gaaatttacg aaatcaaccc caacctctgc acgcagtgcg tcggacacta
    20341 cgatgagccg cagtgccagc aggtttgccc ggtggactgc atcctgattg acgaagaata
    20401 tcccgaaacc catgacgagt tgatggcgaa atacgaaaag attatccagt ttaaataaat
    20461 tctttttaaa acatcaaatt atgtctgttt tgaaataaaa tcaaaaaaaa acttgacgga
    20521 aaagcaagcc gctaataaac taacgttctc ttttggaggg gttcccgagc ggtcaaaggg
    20581 ggcagactgt aaatctgttg cgaaagcttc gaaggttcga atccttctcc ctccaccaaa
    20641 attcttactt ggggcagtag cgagtaatgc gggtgtagct caatggtaga gcagaagcct
    20701 tccaagctta cggtgagggt tcgattccct tcacccgctc caaacaatta ggcccatgta
    20761 gctcaggggt agagcactcc cttggtaagg gagaggccgg cagttcaaat ctgcccatgg
    20821 gcaccatctc tcgattattc atttctttaa ggcttagata tataggatat tg
//
LOCUS       DAWFLT010000028        20679 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWFLT010000028 DAWFLT010000000
VERSION     DAWFLT010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20679
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.028"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(117..545)
                     /locus_tag="WGR74_001067"
     CDS             complement(117..545)
                     /locus_tag="WGR74_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ0905497.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAESPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(610..2358)
                     /locus_tag="WGR74_001068"
     CDS             complement(610..2358)
                     /locus_tag="WGR74_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ0905498.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTVMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKTLYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2685..4118
                     /gene="ccoN"
                     /locus_tag="WGR74_001069"
     CDS             2685..4118
                     /gene="ccoN"
                     /locus_tag="WGR74_001069"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ0905499.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4145..4756
                     /gene="ccoO"
                     /locus_tag="WGR74_001070"
     CDS             4145..4756
                     /gene="ccoO"
                     /locus_tag="WGR74_001070"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ0905500.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4761..4931
                     /locus_tag="WGR74_001071"
     CDS             4761..4931
                     /locus_tag="WGR74_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ0905501.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4955..6052
                     /gene="ccoP"
                     /locus_tag="WGR74_001072"
     CDS             4955..6052
                     /gene="ccoP"
                     /locus_tag="WGR74_001072"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ0905502.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAASEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6092..6286
                     /locus_tag="WGR74_001073"
     CDS             6092..6286
                     /locus_tag="WGR74_001073"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ0905503.1"
                     /translation="MPGIPHVGIKADWADRIKKGKDTLHKHAIEGFNTMPAKGGRGDL
                     SDDEVKAAVDYMVNQSGGKF"
     gene            6488..7897
                     /locus_tag="WGR74_001074"
     CDS             6488..7897
                     /locus_tag="WGR74_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0905504.1"
                     /translation="MDTQIKTEAGQQNNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYAMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPPKGKARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWILELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVVFCLAV
                     IFIGISGCVMWWKRRPSGAVGMVPPAQKIKLPVWWAMAVPLLLIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7999..11208
                     /gene="recC"
                     /locus_tag="WGR74_001075"
     CDS             7999..11208
                     /gene="recC"
                     /locus_tag="WGR74_001075"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ0905505.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSVLQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILS
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVGTDDGSIRIVS
                     AHSPLRELQILKDKLLKILHEYPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVRWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTAMFGRFAAFLETLSDIVRIWRQPATVDEWVGRCRGLLETLFQAEADDQKAVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNLIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAEIYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPLPYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILVEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLATAEAFAQKQDWEAALKKAQTAYHGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLVRLLADTLAAAEKKEEAGEA"
     gene            complement(11278..12846)
                     /gene="mtrF"
                     /locus_tag="WGR74_001076"
     CDS             complement(11278..12846)
                     /gene="mtrF"
                     /locus_tag="WGR74_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ0905506.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDFRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13180..13392
                     /locus_tag="WGR74_001077"
     CDS             13180..13392
                     /locus_tag="WGR74_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905507.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
     gene            complement(13671..14303)
                     /gene="mtrR"
                     /locus_tag="WGR74_001078"
     CDS             complement(13671..14303)
                     /gene="mtrR"
                     /locus_tag="WGR74_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ0905508.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
     gene            14711..15949
                     /gene="mtrC"
                     /locus_tag="WGR74_001079"
     CDS             14711..15949
                     /gene="mtrC"
                     /locus_tag="WGR74_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ0905509.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFIVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
     gene            15961..19164
                     /gene="mtrD"
                     /locus_tag="WGR74_001080"
     CDS             15961..19164
                     /gene="mtrD"
                     /locus_tag="WGR74_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ0905510.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LRATYPGASAQVMEDSVLSVIERNMNGVEGLDYLSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            19219..20622
                     /gene="mtrE"
                     /locus_tag="WGR74_001081"
     CDS             19219..20622
                     /gene="mtrE"
                     /locus_tag="WGR74_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ0905511.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANGSRQGSLSGGNVSSSYNVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPNDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYAAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
BASE COUNT         5018 a         5092 c         5577 g         4992 t
ORIGIN      
        1 gtctcccact gtaaaaacgg agaaggacag aggacggcag taaggtttgc cgtcctcttt
       61 ttcaagttgc ggtggaatgc cgcttgaagg tgtggtttaa acgtttcggc ttgtaatcag
      121 acgcgggcgt tgtccagccg ctctctgaag ttttccaaat ttacgcctgc cgcttgggcg
      181 cggcggatga tttcgtcaac cggcatacct tctgccgccc ggcggaagcc ccacaggagg
      241 gagcagcgcg taccggtccg gcaataggca aggacgggag attcggcttg tccgatgagt
      301 tggcggaagg tttcgacatc gtgtttttgg atgtcgcgtg cggtaacggg ttggtgatgg
      361 aatccagtaa cgcctgcttg ttccagccac tgtttgattt gggcgaagtc gggttgcgat
      421 tcttcttcgc ggtcggggcg gttgcagatg acggttttga tgcccagttg cgcgatttgt
      481 tccgcatcgg ctttggtcag ttgcggagaa atatagaggt gttcgtcaag tttcagaatg
      541 gccattttgt ttcctttact ttgcggttgt ttttcagacg gcattttgtg tgcaatgccg
      601 tctgaaagtt taaccgaaca accagttgtc caacaggttt cggtctgtga tatgtaggat
      661 ggcagtatcc gctgccgctg cctgaaccgt caagacggtt tgacgcaata tgccggcacg
      721 gaagaagtgg atattgattt ttgcattgac gtggtatcgg gcccattgtg cggtaaagtc
      781 ggtgcaggca taaccgtcta aagcaatgat tttgtcttgc gggcacagtg ccgcagattc
      841 cgcgctgccg ccgttgaaga catgggtcag gacgatgtgg tcggtgtttt gtttgaaacg
      901 tgcgccaaaa tcgcctgccg acgggacggg gcagatgtgt tctgcgtatc cgccgccgtg
      961 ttgtcgggga agcggcagga aggtcagtcc cacgcctgcg gttgccaggc attcggcaag
     1021 cggcaaatct tcggtactgt ataacgtttt ttggaaaaaa tcttccaaat ccaagccggt
     1081 aatttcctga cagcggattt gccagtgttt ttccggaata cccgagtgtg tgtccctcca
     1141 ctcccgatag agtttgtcca ttaccgtatc gagagaatgt ctgccgttgc ttcggttgcg
     1201 tattatcaga tcaaggcaca atgcggcaag cgcgcctttc tggtagtagc tgacgatggc
     1261 gttggggctg ttttcatcag gtttgtaaaa tttgttccac gcggtaaaac tcgattccgc
     1321 caaggtctgc ctcaaacggc cgcgggtttg ttgtacgcgc gtaatgcctt gtgccagcag
     1381 gtttaaataa gattcgggcg agatggtgcg gctgcgtgcc aaaaacaaat cgtcgtaata
     1441 ggatgtaatg ccttcgaatg cccatagttg ttcggtatag ttttctttgt cgaggtcata
     1501 agggacgaac gcggcaggtt tgatggattt gacgttccac gcgtgaaaat attcgtggga
     1561 gaaaagtccg agcaatgtgg tgtaggtatc gtcggcatcg gtcataccgt acggcggaag
     1621 gctgtggcgg tcggcgagca gggcggtgct gtcggtgtgt tccaaaccgc cgtaaatatg
     1681 gtcgccgacg tggagcagga acaaatattt ttgaaacggg gcaggggagg aaaacaccgc
     1741 cagttctgtt tcgcagattt ttttgatatc cgaaaccagc ctgttgcggt cgaaatcggg
     1801 atatatgccg cttaaggcaa ttgtgtgcgg aatgcctgcc gcctcaaaat ctaaaaattc
     1861 aatcaagccc atctcgacag gtcggtcaat caattcggca taagatgccg cctgaaagac
     1921 aaacctccct gtttccggca gcgttgtggc aatacgccat tcggacggaa tacccgtcaa
     1981 ttccaagcgg tgcggcagcg tttccgttcc ttcgactttc aaaaacaggc acgatccgtc
     2041 aaaaaaaccg cgttctgtcg tcaggaaaga acctcgaacc gacaaatcga atgcatatac
     2101 ggtgtagcgg atttgccact cgccgcgtac ggcggcggca tgccagcggt ttttggcaat
     2161 ttgttcgagc ggcatggacg tgccgttaca ggatgcatgg atagaagtga tgtggcggga
     2221 aaaatcccga atcagatagc tgcccggaac ccagtttggc aggcttattt ctataggaag
     2281 atcatcatct tgtgtgaatg tcagcaggat gtgccactcg tggtcgagag gggacggggt
     2341 tattttataa tgaatcataa ctatcaaata tatcgttaaa cgggatggat taaatacctt
     2401 taaaatgcag cttaacctac atcaataaag tgccacaaaa agggaaaatt ggaaacaaaa
     2461 gcaatggcgg tcgggttgta atttattata acaatatgat ttatatgtaa taaaaaacag
     2521 attaagcaaa gggctcgtcg gcggcgcgaa tgtaattccg cttggcgtaa taaggcagat
     2581 atgttaaatt tagaaaaaat tacgcttgcc cggattgcac cttaaacagg ctgcggaagc
     2641 ggcaggataa aaatctttat tcccataaat tcatggagac tctcatggac acacaaactt
     2701 acaactacaa agtggtgcgc cagttcgcca tcatgactgt agtttggggg attgtgggta
     2761 tgttggtcgg cgttatcgtc gccgcccagc tttttgctcc tgccctcgac ttgtctaata
     2821 tcggaccttg gttccacttc ggccgcctgc gtccgctgca caccaatgcg gttattttcg
     2881 catttggcgg ttgcggcctg atcggcacat catactacgt tgtccaacgt acttgtaata
     2941 cccgtctttt cggcggttgg ctgccggcat ttaccttttg gggctggcag gcggtaatcg
     3001 ttgccgccgt cgtcagcttc cctatgggtt ggacccaagg taaggaatat gccgaactgg
     3061 aatggccgat cgatattctg attactttgg tttgggtggc ttacgccatc gtattcttcg
     3121 gtacgattgc caaacgtaag attaaacata tttacgttgc caactggttc tacggcggct
     3181 ttattttggc ggtcgcactt ttacacatcg tcaacaatat cagcatcccg gccggtttga
     3241 tgaagtcata ccccgtctat tccggtgcga ttgatgctat ggttcaatgg tggtacgggc
     3301 ataatgccgt gggcttcttc ctgactgccg gcttcttggg tatgatgtac tatttcgtac
     3361 ccaaacaagc agcccgcccc gtttactcct accgcctgtc cgtcgttcac ttctgggcgt
     3421 tgattttcac ctatatgtgg gcgggtccgc accatcttca ctacactgcg ctgcctgact
     3481 ggacgcaatc tttgggtatg gttctgtctt tgattctgtt cgcaccctct tggggcggta
     3541 tgattaacgg catcatgacc ttgtccggcg cgtgggacaa actgcgtaca gacccgattc
     3601 ttaaattcct gattgtatcc ttgtccttct acggtatgtc tacctttgaa ggcccgatga
     3661 tgtcgattaa aacggtcaat gcattgagcc actatacgga ctggaccgtc gcgcacgttc
     3721 atgcgggtgc gttgggctgg gtaggctttg taaccatcgg ttccgtctat tacatgattc
     3781 cccgtctgtt cggcaaagaa cagatgcaca gcaccaagct ggtagaagca catttttgga
     3841 ttgcgaccat cggcgtggtt ctgtatatcg ctgccatgtg gattgccggt gtgatgcagg
     3901 gtttgatgtg gagttctttg aacgatgacg gtacgctgac ctactcgttt gtcgaatccg
     3961 taaaacgcac catgccttac tacgtgatcc gttttgcagg tggtttattg tatctgagtg
     4021 gtatgtgcat tatggcgtac aacgtgtacc gcacagccat cggtggtaaa gcagtcgatg
     4081 ccgaaatccc tgcggtttcc caaacacagc accactaaaa tactaagaaa gataggctac
     4141 caaaatgaaa ttacaacaat tggctgaaga aaaaatcggc gttctgattg tgttcacgct
     4201 gcttgtagtc agtgtcggtc tgttgattga agttgtgccc ttggccttta ccaaggcggc
     4261 aacacagccg gcaccgggcg tgaagcctta caatgccttg caggttgccg gacgcgatat
     4321 ttacatccgt gagggctgtt acaactgcca ctctcaaatg attcgtccgt tccgtgcgga
     4381 aaccgagcgt tacggtcatt actctgttgc cggagagtcg gtttacgacc atcctttcca
     4441 atggggttcc aaacgtaccg gtcctgattt ggcacgtgta ggcggccgct attccgacga
     4501 atggcaccgt atccacctgc tgaatccccg tgatgtcgtg cctgagtcca atatgccggc
     4561 attcccatgg cttgcacgca ataaagtcga tgtcgatgca accgttgcca acatgaaggc
     4621 tttgcgtaaa gtaggtactc cttacagtga tgaggaaatt gcgaaagcgc ctgaggcttt
     4681 ggcaaacaaa tccgagctgg atgctgtagt cgcctatctg caaggattgg gtctggcttt
     4741 gaaaaacgta aggtaacatc atggatatta acggtattcg cgctcttttc acggtatgga
     4801 tctttatctg tttcctgttg gtactctata tcgtcttcaa caggcggaat aagaaaaact
     4861 acgataatgc cgcaaacagc atttttgatg aaaaccaaga tgcgcaagat aagaaaagcg
     4921 aaaaccgtta atattgtgat aacggagcaa aacaatgaac acaacatccc aatttaccag
     4981 taatttctgg aatatatata ttgcagttat tgtcttactg agctttatcg ctttggcttg
     5041 gctgctgctg tctcaaaatg ttgtcaaacg tccgaagaag ggcgaagaag tacaaactac
     5101 gggtcatgag tgggacggca ttgccgaata cgacaatccg ctgccccgct ggtggttttg
     5161 gctgtgtgtt ttgacgtggc tgttcggtat cggttatttg gttatgtatc cgggtgtcgg
     5221 cgactacaaa ggtctgctga aatggaccag ccataaccaa tatgaaaaag aggtcaaaaa
     5281 agccgatgag caatacggca aactgtatgc caagtttgcg gatatgccga ttgaaaaagt
     5341 ggcaaaagac cctcaagcca agcaaatcgc ccaaaacctg tttaacactt attgtatcca
     5401 gtgccacggc tctgatgcta aaggctctaa aggttttccg aatctgaccg atagcgattg
     5461 gttgtggggc ggtgatcccg ataaaatcca cgagaccatc gaaaaaggcc gtgttgcgac
     5521 tatgcctgcc tggggtcctg ctttgggcga agaaggcgtg aaagacgttg cccattatgt
     5581 gatgtccctt tcaaaacccg aaggtcagta tgatgaggaa cgtgccgcgc gcggacaagc
     5641 cttgttcagc ggtccgcctg ccaactgttt cacttgccac ggcgataagg gacaaggtat
     5701 ccaaggtttg ggtccgaacc tgactgatga cgtgtggttg tggggcggta cgcaaaaatc
     5761 cattatcgaa accattacca acggtcgcag cagccaaatg cccgcttggg gacatttctt
     5821 ggataaagac aaactgcata ttatgactgc ttatgtatgg ggtctttccg ataaagacgg
     5881 taaagctccg gtgaaaaaag ccgagcctgc accgactccc gcaccggcgg cagaacccgc
     5941 agcctctgct cctgcagaag cagcacaagc cgcgtccgaa gccaaacctg ccgcagcaga
     6001 acctaaagcc gaggaaaaag ctgcacctgc tgccaaagcg gacggcaaat aggtttatga
     6061 aaccgtttgt gccgcctgcc atggcaatgc gattccgggt attccccatg taggcatcaa
     6121 agccgattgg gccgaccgca tcaaaaaagg caaggacacg ttgcacaaac acgcgatcga
     6181 aggtttcaat acgatgcccg caaaaggcgg tcgcggcgat ttgagcgatg atgaggttaa
     6241 agctgcggtt gactatatgg tcaaccagtc aggcggcaaa ttctaacttg actgagtttc
     6301 cgatattatc ggttatgaaa atccagattc agtattgaat ctggattttt tgttttaacg
     6361 ggcatccggt tttaaacgca gactgcctat ttgttgtaat gaattttgac tttagtcata
     6421 aagaattagt ctttattgtt tataatcgcg catcatcacg atagccctat tttcaaaagg
     6481 acgattaatg gatacacaaa tcaaaactga agccggtcag cagaataacc ggcgttatct
     6541 gaccgtttgg cggtggcatt tttatgccgg tctgctggtt atgccttttc ttaccctgct
     6601 tgccgttacg ggtttgggta tgctgctgtt tgccaatatt accggtaagg agggcgagcg
     6661 gattcatgtt gtgccgcagg caacggtaca acctctgtct gttcaggcgg aagcggcacg
     6721 cagtgccgtt aatccggaaa cttcgtccgt cgttcagtat attgcgccgc gtgccgatga
     6781 tatggttgcc gtgttccgtg tcaacaatga gggcaaggca acgatggtcg cggtcgatcc
     6841 ttatacggca aaagtggtca gtaccatgcc gcgcaatcag ggttggtatt acgcgatgga
     6901 tgaaatccac agcgatatga tgctcggttc gacaggtgat tatcttttgg aaacggctgc
     6961 atcgctgacg attatcatga taatcagcgg tttgtacctt tggtgggtga aacggcgcgg
     7021 catcaaggcg atgctgctgc cgccaaaagg caaggcgcgt tcttggtggc ggaatctgca
     7081 cggcgcgttt ggaacttggg tgtcgttgat tttactgttg ttctgcctgt cgggtattgc
     7141 ttgggcaggt atttggggcg gcaagttcgt gcaggcttgg agtcagttcc cggcaggcaa
     7201 atggggtgtc gaaccgaacc ctgtttcagt cgtgccgacc catggcgagg tattgaacga
     7261 cggcaaggtt aaggaagtgc cgtggatttt ggagcttacg cctatgcctg tttcagggac
     7321 gactgtgggc aaagacggca ttaaccccga cgagccgatg acattggaaa ccgtcgaccg
     7381 ttttgcgcgg gaaatcggtt tcaaagggcg ttatcagttg aatttgccca aaggcgagga
     7441 cggcgtatgg actttgtcgc aagactctat gagttacgat atgattagtc cgtttgccga
     7501 ccgcacggtg catatcgacc agtacagcgg caagattctt gccgacatcc gttttgatga
     7561 ttacaacccg ttcggcaaat ttatggcggc gagcattgcg ctgcatatgg ggactttggg
     7621 ctggtggagc gtgttggcga acgtcgtgtt ctgccttgcc gtcattttta tcggtatcag
     7681 cggctgcgtg atgtggtgga aacgccgtcc gtccggcgcg gtgggcatgg ttccgccggc
     7741 gcaaaaaatc aagctgcccg tctggtgggc aatggcggtg ccgctgctgc tgattgcatt
     7801 gcttttcccg accgcgctgc ttgccattgc cgtgatttgg ctgttggata cgctgctgtt
     7861 gtcgcggatt cctgttttga ggagatggtt taaatgaccc gatgccgtct gaagcgttca
     7921 gacggcattt tgtttgaagg cgggcggggg aaaggctata taatcccgaa tacttgacca
     7981 cagcttctgt ttgaaatcat gttttatctg tatcaatcca accgtcttga aacgctggcg
     8041 gcactgtttg cccgcattca aaaagtcaaa ccgctgaaat cggttttaca gcccgaacag
     8101 attattgtgc agagtcaggg gatgcgccgc tacctcaata cctgcctcgc ccgcgatttg
     8161 ggcgtggcgg cgaatttgtc gttcagcctg cccgccggtc tgacgtggaa gctgatgaaa
     8221 aaactgattc ccggtattcc ggaactcagc ccgtttgcgc ctgaagtgat gcgctggcgg
     8281 ctgttggatt tgttccgcag cgaggcattt cggaatacgg cagaatttga agatgtgagg
     8341 aatgtgctgc aagactatct aggcagcggc gaatcggcag attatcagct tgcgggacag
     8401 cttgcggaca tattcgacca ataccttgtc tatcgtccgc agtggataga cgcgtggcag
     8461 cagggcagga tactcagttt gggcgacgat gaaatctggc agtccaaact gtggcgttat
     8521 cttgatgacg gcaggcagag cgcgccgcac cgtgttgcgt tgtgggaaaa gctgttggaa
     8581 tctttgagca gtgataagct gcccgagcgt tatttcgtgt tcggcatttc cacgatggcg
     8641 ccgatgtatt tgcagctttt gcacaagctg tccgaacatt gcgatgtgtt cgtgtttgca
     8701 ctcaatccga gtgggatgta ttggggcaac gtcatcgaag cggcgcaaat cctcaaaggc
     8761 ggcggcgatc ccgatttaac tcaggcaggg catccgctgc tcgcctcatt gggcaagcag
     8821 gggcgcgact ttttcgactt tttgaacgaa atggaaatag aaggagaaac gccggtattt
     8881 gaagaaggcg ggcgcgatac gcttttgcac gccctgcaaa ccgatattca aaacctgaaa
     8941 atgccgtctg aaaatgtggg aagcgtcggt acggatgacg gttcgatacg tatcgtctcc
     9001 gcgcacagcc ctttgcgcga attgcagata ctcaaagaca agctgttgaa aattctgcat
     9061 gaatatcccg attggcagcc gcatgatatc gccgtattaa ccccgaatat cgaatcctat
     9121 acgcctttta tcgaggccgt gttcggacag gcgcagcccg gtgcgcaggc attgccgtat
     9181 tccgtttcgg acgtgaaaat aagccgccga caaccgctgt ttcatgcttt gtcatgcctg
     9241 ttcgacttgt tggaaagccg atttgaagtc gataaagtgc ttgtgctttt agaaaccgca
     9301 cccgtgttgc gccgtttcgg actgactgag gacgatttgc cgcttttgca cgacatggtt
     9361 gccgatttga acgtccgctg gggtttggac ggagaaatgc gcggcggcac ggatcagctg
     9421 tttacctgga agcaggcggt agaacgcatg atattgggct ggatgctgcc cgaaggcggc
     9481 aatccgatgt ggcaggatgt cagcgcgtgg tatgccgacg tgaatcaaac cgccatgttc
     9541 ggacgttttg ccgccttcct cgaaaccctg tcggatattg tacggatatg gcggcaacct
     9601 gcgacggttg atgaatgggt aggacgttgc cgaggtttgc ttgaaacatt gttccaagcc
     9661 gaagccgatg accaaaaggc agtccaaaac ctcgaaaacg aatgggtcaa atggcaggcg
     9721 gaaacagaat tggcgcaatt ttccggacag ttgccgcaac aaaccgtcat ccgccacatc
     9781 cgccgctttc tcgacagcga aagcgaggca ggctttttac gcggcggcat caccttttgc
     9841 agtatggtgc cgatgcggag cctgcccttt aaagtcatct gtctgttggg tttgaacgac
     9901 ggagattttc cccgcaatac caaagccgcc gtattcgacc tgattgccaa acatcccgcc
     9961 aaaggcgacc gcgcccgccg cgacgacgac cgctacctct tcctcgaagc cctcatcagc
    10021 gcgcgtgaaa tcctgtacct gtcctacatc gggcgcgaca tccgcaaaga cgaagaactg
    10081 gctccgtctt ctttgttggg cgaactcatc gataccgttg ccgctatggc gggcatcggt
    10141 agccgccaac ttgcacaaaa cctgatagaa cagcatccgc tgcaagcctt ctcgcgccga
    10201 tattttcaag aaggcggacg ttcagacggc atattcggca cgcgtaccga ctacgccgcc
    10261 gcgctcggac aaacgcccga accgccgcaa ccctttttcg atcaacccgt agaaaacgcc
    10321 gaacctgttg ccgaaatcgg acaggacgaa tttatccgct tctggcgcaa ccctgtcaaa
    10381 gtatggcttc agcagcagct tgcgtggagc gaaccccata tcggcgaagc ctgggagcct
    10441 gccgaaccct tcgagccgca acacgccgat caaatcgccg aaatctacat cgaagcacgg
    10501 cgcgaaggac gggattttgc ccaaaccgcc gcccgcatcg gggcggaaag cctcctgccg
    10561 tcgggagagt tgggcagact ttggcagcag gacttccaaa ccgctgccaa acaaatcgac
    10621 acggcggttt taaacagtcc caaacttcct ccgctgccct acgccatacc gtcggacggg
    10681 caaatcctga aaggcagctt gggcaatctg taccgctgcg gacaagtgtt ttacgcctac
    10741 ggcaaaccca acgcgccgca acgtatcgct tttttgttgg aacacctgat attttgcgcc
    10801 gttatgccgt ctgaagccga aacgcggcaa acctttatcg tccaatccgg agaaaccgaa
    10861 atattggtgg aaatcgcgca agacagggca ttgcagctat tgtcggaatg gatggcgttt
    10921 ttcaatatcg ggcaaaaccg cccgctgccg ttttttgcca aaacctcgct tgccaccgcc
    10981 gaagcgtttg cccaaaaaca agattgggaa gccgccctga aaaaagccca aaccgcctac
    11041 cacggcaaca aagtcagcaa agggcagaaa gactataccg aagtcgccct cgtgttcggc
    11101 aacgcaagcc aaaacccgct cgaacagccc ctgtttgaaa acctcgtccg tctgcttgcc
    11161 gacacgcttg ccgcagcgga aaaaaaggaa gaggccggag aagcctgacg ggatggaaag
    11221 gcaaatgccg tctgaaaaca gcaaaaaggt tttcagacgg cattttgttt atcgtgttta
    11281 aggtgcggga tagaatgtgg gcgcgccggg accgacgggc aggcccaaaa caaataccca
    11341 aatgcagaat aaggcaatcc acgcaatcaa gaagaaagcg gaatacggca acatcataga
    11401 aatcagcgta cccacgcccg catctttttt gtatttgatc accgtcgcca taatcagccc
    11461 gaaataactc atcatcggcg taataatatt ggtaacggaa tcaccgatgc ggtaagcggc
    11521 ttgaatgact tcgggcgcgt agccggccaa catcagcata gggacgaaaa tcggcgcagt
    11581 taccgcccat tgcgcggagg cggagcctat catcagattg ataaaagcac aaattaaaat
    11641 aaaaccgata aacaacacgc tgccgcccaa gccgacttct tttaagaacg tcgccccttt
    11701 aacggcaata tattgcccaa tattcgtcca attaaaaaat gcgacaaact gtgcggcaaa
    11761 aaagatgatg accaaataaa gccccagagt actcatcgat tcggccatcg cattaacgac
    11821 ttcctgttcg ccgcgcaaac ttcgggttac ccggccataa acaatgcccg gcagtgcaaa
    11881 caacaagaaa ataaaaacaa caatcgattt taaaaacggc gaaccggaaa ccaatcctgt
    11941 ttcaggatga cgcaaaatac cgtcggcagg gacgatgctc caagccaata gggcggataa
    12001 ggcaacaaac accacgccag cccaaattaa tcctttatat tccaaaggcg tgatttcatt
    12061 ggaatgccga aagtcttttt cttcttgtga caaatctgat tgataagggc ccaattgcgg
    12121 ttcgacgatt ttttcagtaa caaaataacc aatcaaagca atcacaaacg tactggctac
    12181 cataaaaaac cagttggctt cagggcctac gacgtagtcg ggatggatga tttgcgccgc
    12241 ctgttgggtg atgcctgcca agagcggatc gattgtgcct aagaacagat tggccgaata
    12301 accgcccgaa acgccggcga aagccgcagc cagaccggca agcggatggc ggccgaggga
    12361 atgaaagatg atggcggaca aagggattag gacgacatag cccaattcag aagcggtatt
    12421 agataaaatc cctgtaaaaa caaccataaa agtagtgagt ttgcgtggag attttgtgag
    12481 caataagcgc attaatgcgg aaatcaagcc cgatttttcc gcaatcccca cgcccaataa
    12541 agaaaccaac accgttccca acggcgcgaa accggtgaaa tttttaacgg tatgcgtcag
    12601 gattttgatc aaaccgtcag catcgagcag gctgacaacg tgaatcaaac cgtcatcggc
    12661 acgtcctttc gcaccaacag ggcgcggatc ggggacggat agtccgaaat acgcaccggc
    12721 ggcagaggca atcagcaata acacaatgaa aataataaaa agcgtaaccg ggtgcggcaa
    12781 catattgccc agccattcga ctgtgcgtaa aaatcgtccg tcccgttgcg tatcggtttg
    12841 actcatctgc ttctccttaa aaaaaatata ttgagtgcat taaatttttg aatgtaacat
    12901 gaagtgtttt gtaagcaaac taaaataatc gtatatgtat ataaccgtaa tagattaaaa
    12961 cagaaaatgc ccacatcgtt attctcataa aggcggtaat ccgaaaactt gacgttaaag
    13021 ccttatcagg aatgactgga actcaacaaa gcggtttccc gctttcgcgg aaatggcggg
    13081 attttcggtt tttgtttgca aaaatgccgg agaaaaatgc agtttacatt ttgaatggta
    13141 ttgttattaa ttgtaaataa atattggaag gtaaatgata tgaaaaatga aattcaaaaa
    13201 attatggaca aatatgatcc atggcatgaa gatgattttg agtcgtatga agacattgcc
    13261 aaagatgtat cgttgatgac agataaaacg ttcattgaac attatttgtt agaagtttat
    13321 tcagaagaaa acggacattt tgaccaagag aatgtccatg caatgataga agaaattaaa
    13381 aatgcaattt agtgaatatg agacctttgc aaaaatagtc tgttaacgaa atttgacgca
    13441 taaaaatgcg ccaaaaaatt ttcaattgcc taaaaccttc ctaatattga gcaaaaagta
    13501 ggaaaaatca gaaaagtttt gcattttgaa aatgagattg agcataaaat tttagtaacc
    13561 tatgttattg caaaggtctc aatatgtatt tttagaagaa ggctttgtgt cattttgatg
    13621 ccgtctgaaa gggtttgttc gtttcagacg gcattgttac caaggcttga ttatttccgg
    13681 cgcaggtcgg gatggttttc caagttgtcc atcattatcc cgatgatgcg cggggcggtt
    13741 ttgcccaaat cgaaacgttc gcaggaagag aaccaccgcc aaatcagccc gtccaaggtt
    13801 gatttgatga agatgaccgc tgtttccttg tccaaatcgt cagccaaatc ctgattttcc
    13861 accgcttcgg tcaaaacggc ggtaattttc tcgcgccaga ttgcctgatg cttgcgggca
    13921 atggcgataa cggcggcgtt ttgttccgtg tgttcgcatt ttaaaaacag gatgttgtgg
    13981 aatttgtagt agatgtcgtt gctttgcagc cgctcgaaaa agtgcagcag cgtgtggcgg
    14041 aataccgccc aagaccctcc ttcggcatct tcggcatctt gcgcgatgca gttttcgatg
    14101 tcgtcgcaga tacgttggaa cagcgcgtcg aacaagtctt ccttattttt gaaatgccaa
    14161 tagagcgcgc cgcgcgttac gccggcggct tgggcgattt cgttgagcga ggtgcgcgca
    14221 atccctttgc ggtaaaaggt ttccaaggcg gcaagcatca ggtgttcttt ggttttcaag
    14281 gcttcggttt tggtttttct cataatggcg ttttcgtttc gggtcggttt gatgagggcg
    14341 gattataaaa aagactttgt aaccatgcaa tcgtgtatgt ataatgaaac ccatgaaatt
    14401 gagactacat ctcaactttg aaaacaggca taacctgctt tgcaacccgt tcgaacattc
    14461 gggtatcaaa gcaggcggtt tttatatcct gaaatatagt ggattaacaa aaatcaggac
    14521 aaggcgacga agccgcagac agtacagaca gatagtacgg aaccgattca cttggtgctt
    14581 gagcacctta gagaatcgtt ctctttgagc taaggcgagg caacgccgta ctggtttttg
    14641 ttaatccact ataagaatgc ctgttgattg gcaaaaccct ttacatcaaa ccagacagat
    14701 aggataaata atggcttttt atgcttttaa ggcgatgcgt gcggccgcgt tggctgccgc
    14761 cgttgcattg gtactgtcgt cttgcggtaa aggcggagac gcggcgcagg gcgggcagcc
    14821 tgctggtcgg gaagcaccgg ctcccgtcgt cggtgtcgta accgtccatc cgcaaaccgt
    14881 cgcattgacc gtcgagttgc cggggcgttt ggaatcgctg cgtaccgccg atgtccgcgc
    14941 ccaagtcggc ggcatcatcc aaaaacgcct gttccaagaa ggcagttatg tccgtgccgg
    15001 acagccgctg tatcagatcg acagttccac ttatgaagca ggtctggaaa gcgcacgcgc
    15061 gcaactggca acggctcagg caacgcttgc caaagcggat gcggatttgg cgcggtacaa
    15121 gcctttggtt gccgccgaag ccgtcagccg gcaggaatac gatgctgcgg taacggcgaa
    15181 acgttctgcc gaggcaggtg tcaaagcagc acaggcggca atcaaatctg ccggcattaa
    15241 tctgaaccgt tcgcgcatta ccgcgccgat ttccggcttt atcggtcagt ccaaagtttc
    15301 cgaaggtacg ctgttgaatg cgggcgatac gaccgtgctg gcaaccatcc gccaaaccaa
    15361 tccgatgtat gtgaacgtta cccagtctgc atccgaagtg atgaaattgc gccgtcagat
    15421 agccgaaggc aaactgctgg cggcggatgg tgtgattgcg gtcggcatca aatttgacga
    15481 cggcacagtt taccctgaaa aaggccgcct gctgtttgcc gatccgaccg tcaacgaatc
    15541 gaccggtcag attaccctgc gcgccgccgt accgaacgat cagaatatcc tgatgcccgg
    15601 cctgtatgtg cgcgtgctga tggaccaagt ggcggtggat aacgcattca tcgtgccgca
    15661 gcaggcggta acgcgcggtg cgaaagatac cgtgatgatt gtgaacgccc aaggcggtat
    15721 ggaaccccgc gaggtaacgg tcgcgcaaca gcagggtacg aattggattg ttacgtcggg
    15781 tctgaaggac ggggacaagg tggttgtgga aggcatcagt atcgccggta taacgggtgc
    15841 gaaaaaggta acgcccaaag aatgggcgcc gtctgaaaac caagccgccg cgcctcaatc
    15901 cggcgttcag acggcatctg aagccaaaac tgcttctgaa gcggaataag gaaggcatcg
    15961 atggctaaat tttttatcga ccgccccatt ttcgcgtggg ttatttcgat tttcattatt
    16021 gcggcgggta ttttcggcat taaaagcctg cctgtttcgc aatatccgtc tgtcgccgcc
    16081 ccgaccatca ccctgagggc cacttatccg ggcgcgtccg cgcaggtaat ggaagacagc
    16141 gtgctttccg tgatcgagcg gaatatgaac ggcgtggaag gtttggatta tctgtccact
    16201 tctgccgatt cgagcggcag cggcagcgtg agcctgacct ttacgcccga taccgacgag
    16261 aatctggcgc aggtggaagt gcagaacaag ctttccgaag tattgagcac gctgccggca
    16321 accgtccagc aatacggcgt aaccgtatcc aaggcgcgtt ccaatttcct gatgattgtg
    16381 atgctttcgt cggatgtgca gtcaaccgaa gagatgaacg actacgcgca gcgtaatatc
    16441 gttcccgagt tgcagcgtat cgaaggcgtg gggcaggtac gcctgttcgg cgcgcaacgc
    16501 gcgatgcgga tttgggttga tcctaagaaa ctgcaaaact acaatttgtc gtttgccgat
    16561 atcggcagtg cgctgtccgc ccagaacgtc cagatttcag cgggttctat cggttcgctt
    16621 cccgccgttc gcggacagac ggttacggct accgtaacgg cgcaagggca gttgggtacg
    16681 gcagaagaat tcggcaacgt catcctccgc gccaataccg acggttctaa tgtttacctg
    16741 aaggatgtgg caagggtcgg actgggtatg gaagactatt cttcctcaac ccgtctgaac
    16801 ggtgtaaata ccaccggtat ggcggtgatg ctgtccaaca gcggcaatgc gatggcgacg
    16861 gcaaaggcgg ttaaagaacg catggcgacg ttggaaaaat actttcctca gggtatgagc
    16921 tggaaaaccc cttacgatac ttccaaattc gtcgaaattt cgattgaaaa agtgattcat
    16981 actttaatcg aagcgatggt gctggtgttt gtcgtaatgt atctcttcct gcaaaacatc
    17041 cgttatacgc tgattccgac catcgtcgta ccgatttcgc tgttgggcgg tttcgccttc
    17101 atctcttata tgggcatgtc gattaacgta ctgaccatgt ttgcgatggt tttggtcatc
    17161 ggcatcgtgg tcgatgacgc gattgtggtg gttgaaaacg tcgagcgcat tatggcgggt
    17221 gaaggcttgc cgcccaaaga agcgaccaaa aaagcgatgg gtcagatttc gggcgcggtc
    17281 atcggtatta ccgccgttct gatttccgtg ttcgtaccgt tggcgatgtt cagcggggcg
    17341 acgggcaata tttacaaaca gtttgccctg acgatggcgt catcaatcgc attctccgcc
    17401 ttccttgcgc tgacccttac ccctgctttg tgtgccacaa tgttgaagac aatcccgaaa
    17461 gggcatcacg aagagaaaaa aggtttcttc ggctggttta acaagaaatt caacagttgg
    17521 acgcacggtt acgaaggccg ggttgccaaa gtgctgcgta agactttccg catgatggtt
    17581 gtctatatcg gcttggcggt tgtgggcgtg ttcctgttta tgcgcctgcc gacttcattc
    17641 ctgccgaccg aagaccaagg cttcgtcatg gtcagcgtgc aactgcctgc aggagcgacc
    17701 caagagcgca ccaatgcgac tttggcgcaa gttacccaac tggcgaaaag cattcctgaa
    17761 atagaaaaca tcattaccgt ttccggcttc agcttttcgg gcagcggtca gaatatggcg
    17821 atgggttttg ccatattgaa agattggaac gagcgtaccg cgcccggcag cgatgccgtt
    17881 gcgattgccg gcaagctgac gggtatgatg atggggacgc ttaaagacgg ttttggcatc
    17941 gccgtcgtcc cgcctccgat tctggagttg ggcaatggtt cgggcctgag catcaacctg
    18001 caagaccgca ataataccgg ccataccgca ttgctggcga agcgcaacga gttgattcag
    18061 aaaatgcgtg ccagcggttt gtttgacccc agcaccgtcc gtgctggcgg tttggaagac
    18121 tcgccgcagt tgaaaatcga catcaaccgt gccgccgcgg cggcgcaagg catttcgttt
    18181 gccgacatcc gcaccgcatt ggcaagtgcg ctgagttcgt cttatgtcag cgacttcccg
    18241 aaccaaggtc gtctgcaacg cgtgatggtg caggccgacg aggatgcccg tatgcagcct
    18301 gccgatattt tgaacctgac cgtgccgaac aaatccggtg tcgccgtacc gctttccacc
    18361 attgctactg tttcttggga aaacggtacg gaacagagcg tacgcttcaa cggttatcct
    18421 tcgatgaagc tgtccgcttc gcctgcaacc ggcgtttcca ccgggcaggc tatggcggcg
    18481 gttcaaaaaa tggttgacga attgggcggc ggttacagct tcgaatgggg cggacagtcg
    18541 cgcgaagagg caaaaggcgg ctcgcaaacc ctgattttgt acggtttggc ggttgcagcc
    18601 gtatttttgg tacttgccgc gctttatgaa agctggtcga ttccgctggc ggtcatcctt
    18661 gtgattccgt tgggtttgat cggtgcggct gcgggcgtaa ccgggcgcaa cctgtttgaa
    18721 ggactgttgg gcagcgttcc ctcgttcgcc aacgacatct actttcaagt cggtttcgtt
    18781 accgtgatgg gtttgagtgc gaaaaacgcg attttgatta tcgagtttgc caaagacctt
    18841 caagcgcaag ggaaaagcgc ggtcgaagcc gctttggaag ccgcccgcct gcgtttccgt
    18901 ccgattatca tgacctcgtt cgcctttatt ttgggcgtgg ttcccctgta tattgccggc
    18961 ggtgcaagtt ctgccagcca gcgcgccatt ggtacaaccg tattctgggg gatgctcatc
    19021 ggcacgctct tgtccgtgtt ccttgttccg cttttctatg tggtggtgcg caaattcttc
    19081 aaagaaactg cacacgaaca cgaaatggca gtaaaacacg ccgccgaagc gggcattacc
    19141 ggttcggacg acagccaaca ttaagcaacc atgccgtctg aacgcccacg ggttttcaga
    19201 cggcatcagg acttttttat ggatactaca ttgaaaacta ccttgacttc tgttgcagca
    19261 gccttcgcat tatccgcctg caccatgatt ccccaatacg agcagcccaa agtcgaagtt
    19321 gccgaaacgt ttaaaaacga taccgccgac agcggcatcc gcgccgtcga tttgggttgg
    19381 cacgactatt ttgccgaccc gcgcctgcaa aagctgatcg acatcgcact cgagcgcaat
    19441 accagtttgc gtaccgccgt attgaacagc gaaatctacc gcaaacaata catgattgaa
    19501 cgcaacaacc ttttgcccac gcttgccgcc aatgcgaacg gctcgcgcca aggcagcttg
    19561 agcggcggca atgtcagcag cagctacaat gtcggactgg gtgcggcatc ttacgaactc
    19621 gacctgttcg gacgcgtccg cagcagcagc gaagcagcac tgcaaggcta tttcgccagc
    19681 accgccaacc gcgatgcggc acatttgagc ctgattgcca ccgttgccaa agcctatttc
    19741 aacgaacgtt atgccgaaga agcgatgtct ttggcgcagc gtgttttgaa aacgcgcgag
    19801 gaaacctaca agctgtccga attgcgttac aaggcaggcg tgatttccgc cgtcgcctta
    19861 cgccagcagg aagccctcat cgaatccgcc aaagccgatt atgcccatgc cgcgcgcagc
    19921 cgcgagcagg cgcgcaatgc cttggcaacc ttgattaacc aaccgatacc caacgacctg
    19981 cccgccggtt tgccgttgga caagcagttt tttgttgaaa aactgccggc cggtttgagt
    20041 tccgaagtat tgctcgaccg tcccgacata cgcgccgccg aacacgcgct caaacaggca
    20101 aacgccaata tcggtgcggc acgcgccgcc tttttcccat ccatccgcct gaccggaacc
    20161 gtcggtacgg gttctgccga attgggtggg ttgttcaaaa gcggcacggg cgtttggtcg
    20221 ttcgcgccgt ctattaccct gccgattttt acctggggta cgaacaaggc gaaccttgat
    20281 gtagccaagc tgcgccaaca ggcacaaatc gttgcctatg aatccgccgt ccaatccgca
    20341 tttcaagacg tggcaaacgc attggcggcg cgcgagcagc tggataaagc ctatgacgct
    20401 ttaagcaaac aaagccgcgc ctctaaagaa gcgttgcgct tggtcggcct gcgttacaag
    20461 cacggcgtat ccggcgcgct cgacttgctc gatgcggaac gcagcagcta tgcggcggag
    20521 ggtgcggctt tgtcggcaca actgacccgc gccgaaaacc ttgccgattt gtacaaggca
    20581 ctcggcggcg gattgaaacg ggatacccaa accgacaaat aaccggtcgg gcaataaaat
    20641 gccgtctgaa acgataaggt ttcagacggc atttctttc
//
LOCUS       DAWFLT010000029        19946 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWFLT010000029 DAWFLT010000000
VERSION     DAWFLT010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19946
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.029"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(158..358)
                     /locus_tag="WGR74_001082"
     CDS             complement(158..358)
                     /locus_tag="WGR74_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905512.1"
                     /translation="MPSEELSDGIFAFVRDIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVMPPLGFQTASYRTVKP"
     gene            complement(372..926)
                     /locus_tag="WGR74_001083"
     CDS             complement(372..926)
                     /locus_tag="WGR74_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905513.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSAHPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            complement(1011..1511)
                     /locus_tag="WGR74_001084"
     CDS             complement(1011..1511)
                     /locus_tag="WGR74_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905514.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            1915..3108
                     /locus_tag="WGR74_001085"
     CDS             1915..3108
                     /locus_tag="WGR74_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEZ0905515.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGIVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVRSQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCVL
                     DAPYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDTAEGLRRLEHVLTEFA
                     AANRP"
     gene            complement(3173..4417)
                     /gene="clpX"
                     /locus_tag="WGR74_001086"
     CDS             complement(3173..4417)
                     /gene="clpX"
                     /locus_tag="WGR74_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ0905516.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            4608..4979
                     /gene="rbfA"
                     /locus_tag="WGR74_001087"
     CDS             4608..4979
                     /gene="rbfA"
                     /locus_tag="WGR74_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ0905517.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTVLNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            4990..5514
                     /locus_tag="WGR74_001088"
     CDS             4990..5514
                     /locus_tag="WGR74_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905518.1"
                     /translation="MNNLIPEHLAAYAHSDSLQIEGVHRCFSLSCQGRDTFHIRYYGE
                     PFDGLMTDTDKAPVKIVAVEAVSGDEIVLFDGAEHGYNAMFCDKYSPNQKQNRTLTDL
                     DEYTYRVLIHLYYNIDYEDEYEDFVNSEGQVPLIDGRIISFDSLKRNGFDAISVDLID
                     EKHSVRELLNEELS"
     gene            5572..6492
                     /gene="truB"
                     /locus_tag="WGR74_001089"
     CDS             5572..6492
                     /gene="truB"
                     /locus_tag="WGR74_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ0905519.1"
                     /translation="MTNKPAKRPVNGVLLLDKPEGLSSNTALQKTRHLFRAEKAGHTG
                     VLDPLATGLLPVCFGEATKFAQYLINADKAYTATLKLGEASSTGDAEGEIVATARADI
                     SLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKARDITVYAIDIA
                     EFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEAL
                     ANLDETERDSLLLPCDVLVAHFPQTVLNDYAVHMLRCGQRPRFEEDLPSDTPVRVYTE
                     NGRFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            6812..8958
                     /locus_tag="WGR74_001090"
                     /pseudo
     CDS             6812..8958
                     /locus_tag="WGR74_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            8948..11728
                     /locus_tag="WGR74_001091"
     CDS             8948..11728
                     /locus_tag="WGR74_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ0905520.1"
                     /translation="MANNKTLFDWVEDRKSTLEEIEQTDFFALPEFVERNLKYPFFEW
                     QKSALENFVIFDRASKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIDPAHAKFLFTEKILQGDTVIPIRKVEMFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHKLNLVMLGDEAHHLNAQTKGKKQDELDLEVELKA
                     NAGNAEIERKGWEHMVLELLLNKNGNPGQNVLLEFTATLPENADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLNKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYSAFLNWAENVQADDFEFLDTFSGSLNNSDNANEQGKTRTEQALKFMQENG
                     FEFAHLANWVKQNYQKHNVIITNSETNKNKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNKDFRELLIWANKKIPNPNAKSNNADSLQANPQTLSFQVHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSKLD
                     IQNRNELQNNLLKDWQIEFLGLGQDKQVRLDDKLAGCLKILEMVEKHLNESDMPFIGT
                     KEFTPKKLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISARL
                     GDLKSKYDVHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVKDFLHYQIFI
                     EPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTES
                     FPLGAASLEKMK"
     gene            complement(11872..13044)
                     /locus_tag="WGR74_001092"
     CDS             complement(11872..13044)
                     /locus_tag="WGR74_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ0905521.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALV
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            13334..13780
                     /gene="iscR"
                     /locus_tag="WGR74_001093"
     CDS             13334..13780
                     /gene="iscR"
                     /locus_tag="WGR74_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ0905522.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            13809..15023
                     /locus_tag="WGR74_001094"
     CDS             13809..15023
                     /locus_tag="WGR74_001094"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ0905523.1"
                     /translation="MTVKTPVYLDYAATTTVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WEAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILVSVMWANNEIGVV
                     QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYINGDLEHRVPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            15284..15670
                     /gene="iscU"
                     /locus_tag="WGR74_001095"
     CDS             15284..15670
                     /gene="iscU"
                     /locus_tag="WGR74_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ0905524.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            15757..16077
                     /gene="iscA"
                     /locus_tag="WGR74_001096"
     CDS             15757..16077
                     /gene="iscA"
                     /locus_tag="WGR74_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ0905525.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(16037..16258)
                     /locus_tag="WGR74_001097"
     CDS             complement(16037..16258)
                     /locus_tag="WGR74_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ0905526.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKHGSFRRSRRSL"
     gene            16340..16840
                     /gene="hscB"
                     /locus_tag="WGR74_001098"
     CDS             16340..16840
                     /gene="hscB"
                     /locus_tag="WGR74_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ0905527.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            16938..19688
                     /gene="gyrA"
                     /locus_tag="WGR74_001099"
     CDS             16938..19688
                     /gene="gyrA"
                     /locus_tag="WGR74_001099"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ0905528.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            19845..>19946
                     /locus_tag="WGR74_001100"
     CDS             19845..>19946
                     /locus_tag="WGR74_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0905529.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGVN"
BASE COUNT         5596 a         5022 c         4663 g         4665 t
ORIGIN      
        1 aaaccctttt ccgctttggc aacgattgaa aatttccgta aattcaaata tctagattcc
       61 ttcctgcacg ggaatgacac ggaagggttt cagatgcagg gtgggcattc ctgcccacca
      121 aatcccgccc ttgcaacggt gggcaagaat gctcgcccta cggcttgact gttcgatatg
      181 atgccgtctg aaaacccaac ggcggcatga caatgccgcc ctgccaacgc acgtaaatca
      241 gaattgccat cccgacatca aacgcttgga aacaaaatgc cgtctgaaaa tcaaacggca
      301 acataacaat gtccctaaca aatgcaaaaa tgccgtctga aagctcttca gacggcattg
      361 gtgcgccggg tttaccgcct cctgccgaaa ccgcgcatag cggggcggcg gtaattggcg
      421 ggcgggcgcg ttgtcgggcg gtaacgctgc gcctgcgccg cctgttgttt tgcacggagg
      481 ctgcgcgtgt tcaaatccct gctggtgcgc gcattgggat gtgcggactg atggtaggcg
      541 gcacgcgcgc gccgggcgac gggactgtct tggttgcctg cccgtgtgaa tttgtttgcc
      601 agcgcgttgc cgataaacgc gcctgccgcc gcgccgacca ggctttgcag cagccagctt
      661 cctgtcgatt ggtcgtaaat atactgctgc ccgtctttac cggtaacggg ttgcccgttg
      721 ttgccgtttg cctgtgcttc ggcaggaatg gtgtctttga ctgcttcggg agtcagttgg
      781 taaaccgtat cgtctgcctg ctgtgcgagc tgctgttgca gggcttcaat ctgtttctgc
      841 tgctgttcga gccgcgcctg cgtgtcgtct tggcaggcgg cgagtgcgaa tgttgcgata
      901 agcgcggagg cgatgatttt tttcatgtgt gtcctgtttg ggtggaaaat cggttttatt
      961 gtatcgccgt cgggaatttt ggcaagcatt ctgccgacaa atcgtgatgt ttacaggggc
     1021 agggtgtgca gtttgcggac aaatgcgagg ctgttggcga ctgggttgcc tttgttttcg
     1081 acttcgtgtt cggtttctac ggtcagcagg cggcggtttt tgtgtttgtt caggctcaat
     1141 tcggcttgtg cgggtgtgaa aaacaggcgg tgtttttcgg caaagcggcg ggcgcggctg
     1201 ttggcggttt tcaaaatctg aagcagcgat acgtccagat ggaagcgtcg cacgcccaat
     1261 acgagaatcc gtgcggcaaa aaaatcggcg gcatcggtaa actcggcggg gctgctgcgg
     1321 ctgaagtcgt gccgttcggc ggctatcagg gcggcaacag tgcggatttg cggaatcatc
     1381 gattcgctga tgagtgtgtc gtcccgccct gcatcgagaa gcatggggga aaaatcctgt
     1441 gcatcatcga caataatgct acaagtgtgc agggtttcgt tttgtgcggc ggtttggggc
     1501 attgcattca tggtcatttt cctgattctg tcgtgtgttg ccgaatcggg cgacctgtgt
     1561 gaaggtaaca aaaaagccgc cccgttttcg agcggcctgt tttgcgtatg ggatggattt
     1621 caagcaagcg caaaaaagta ccgcacgtct gtgtggtacc aatagcaata agcggttgta
     1681 aattttttgc cttgcatgat gaaatgccgt ctgaagataa aaatattggg gagattctaa
     1741 atcaaaacgc tgccgcgcct caagcatttt atcgaaattt ttttgatttt tcacctatcc
     1801 gattgaaaat atttcggttt atttttaccg ctgcccgata ttgtcggcaa tttcccttta
     1861 tctgctttga aaaacggtgc ataatcccga gcaaaaccgc aatcaggagc aattatgcaa
     1921 aactatctga cccccaattt cgcctttgcc ccgatgattc ccgaacgcgc ttcaggcagc
     1981 cgcgtttggg atacggaagg gcgtgaatat attgattttt caggcggtat cgccgtcaat
     2041 gcgctgggac actgccaccc tgcccttgtc gatgctttaa acgcgcagat gcacaagctg
     2101 tggcacattt ccaatatcta tacgacgcgt ccggcgcagg aattggcgca aaaattggtt
     2161 gcaaacagtt ttgccgacaa ggtttttttc tgcaactcgg gcgcggaagc gaatgaggcg
     2221 gcgttgaagc tggcaaggaa atatgcgcgc gaccgtttcg gcggaggaaa aagcgaaatc
     2281 gtcgcctgta tcaacagttt ccacggacgc acgctgttta ccgtgtccgt cggcggccag
     2341 ccgaaataca gcaaggatta tgcacccctg ccgcaaggca ttacgcacgt tccgttcaac
     2401 gatattgccg cgctggaagc tgccgtcggc gaacagacct gcgcggtcat catcgagccg
     2461 atacagggcg aaagcggcat cctgcccgcc actgcggaat atttgcaaac cgcgcgccgt
     2521 ctgtgcgacc ggcacaatgc gttgttgatt ttggacgaag ttcaaaccgg gatggggcat
     2581 acgggcaggc tgtttgccta tgaacattac ggcattgttc ccgatatttt gagttcggca
     2641 aaagccttgg gctgcggctt tccgatcggc gcgatgctgg cgacagaaaa gattgccgcc
     2701 gccttccaac cgggcacgca tggctcgact ttcggcggca acccgatggc gtgcgcggtc
     2761 ggcagccgcg ccttcgacat catcaacgcg ccggaaacct tgcacaacgt ccgcagccag
     2821 gggcagaaac ttcagacggc attgctggat ttgggcagga aaacaggctt gttctcacag
     2881 gttcgcggga tgggactgct gctcggctgc gtgttggacg cgccttatcg cggacgcgca
     2941 tccgaaataa ccgccgccgc cttgaaacac ggcgtgatga ttttggttgc gggtgcggac
     3001 gtattgcgtt tcgcgccttc gctgctgttg aacgatgagg atacggcgga aggtttgcgg
     3061 cgtttggaac acgtgctgac ggaatttgcc gccgcaaacc gcccctaggc atcaaaaaca
     3121 aaaaaccgct tcggaaacgt ggttcaacgt tccgaagcgg ttttgtttgc catcaggact
     3181 cgaacaccaa ttccggttcc ctgccctctt cgatgactgc cttaccgacc accacttttt
     3241 tcaagccttg caaatcaggc aggcggtaca tcgtatcgag caggcagcgt tcgacgatgg
     3301 acctcaggcc gcgcgcgccg gttttgcgct ccattgcctg acgcgcgatg gaacgcaatg
     3361 cgccttcttc aaattccaac tcgacatttt ccataccgaa caaggcttga tactgcttga
     3421 ccaaagcatt tttcggctcg gtcaaaatat taatcagcgc gtcctcatcc agttcttcta
     3481 gtgttgcaat cacgggcaaa cgtccgatta attccggaat cagaccgaat ttaatcaagt
     3541 cttccggttc gacgatgccg aacagcttgg taatgtcggc attttcgtcc ttgctgtgaa
     3601 cggacgcacc gaaaccgata ccgccttttt cagtacgctg gcggatgact ttttccaagc
     3661 ctgcaaacgc gccgccgcag ataaacagga tgttggtggt atcgacgttg ataaattcct
     3721 gattcggatg cttgcggccg ccttggggcg gaacgctggc cactgtacct tcaatcagtt
     3781 tcaacaaggc ttgttgcaca ccttcgccag atacgtcgcg ggtaatcgac gggttgtcgc
     3841 ttttgcgtga aattttatcg atttcgtcga tatagacaat gccgcgctgg gctttttcga
     3901 catcgaaatc acatttgccc aacagtttgg taataatctg ttcgacatct tcgccgacat
     3961 aacctgcttc ggtcagcgtg gttgcatctg ccatcacgaa cggcacatcc agtttgcgcg
     4021 ccaaagattg cgccagcagg gttttacccg atccggtcgg gccgataagc aggatgttgg
     4081 atttcgacaa ttcgacatta gctccggctt tcgggtggcg caggcgtttg taatggttgt
     4141 acaccgacac cgccaaggct ttcttggctt gttcctgacc gataacgtgg tcgttgaggt
     4201 tggcgacgat ttcggcgggc gtgggcagct tgccggattc ttccggctcc cctccggcac
     4261 tttccgaagg cgtgccgtca tttccgtctt catgcaatat ttcaatacag tttgagacgc
     4321 attcgtcaca gataaaggcg ttttcgccct caattaaatg tttgacgtgt gatttggatt
     4381 ttccgcaaaa ggaacaggta cggttttcgt tggacatggc tttctttaca atgtatgcgt
     4441 tacagaaaac ggcacgtgcc gttcgggttg ccaagtataa taactatatc cgttcttatc
     4501 aatgtattac cttaaaatcc cgccgattag gctataatac gccctttcgc aaccgccccg
     4561 gcggcaaaaa tgccgtctga aaccaaatct gaaatctgag gatattcatg agaaaacccc
     4621 aacgcggcta cgcccgccaa gaccgtgtca aagaacaaat catgcgcgag cttgccgaac
     4681 tcgtccgtac cggactgaaa gacccccgcg ccggcttcat caccgtcaac gaggtcgaag
     4741 ttacccgcga ttacagccac gccaccgtgt tctacaccgt tttaaaccaa gatgcgcgcg
     4801 aaattacgga agaagtgctg gaacacgcac gcggacacct ccgcagcgaa ttggccaaac
     4861 gcatcaagct gttcaaaatc cccgagctgc atttcaaata cgacgaatcg ttggaacgcg
     4921 gtttgaacct gtccgccctt atcgatcaag tggcggcgga aaaaccggtt gaagactgat
     4981 actgctaaaa tgaacaatct gattcccgaa catttagccg cctatgcaca tagtgacagc
     5041 ctgcaaattg aaggcgtgca tcgttgcttt tcattatcct gccaaggtag agatactttc
     5101 cacatccgtt actatggaga gccttttgat ggattgatga ccgatactga taaggctccg
     5161 gtaaaaattg tggcagtaga agctgtaagc ggcgatgaaa tcgtattgtt tgatggcgcg
     5221 gaacatggct ataacgctat gttttgcgac aaatatagtc caaatcaaaa gcaaaacaga
     5281 acgttaactg atttggatga gtatacttac cgagttctga tccatcttta ttacaatata
     5341 gactatgaag atgaatatga agatttcgtc aattctgaag gacaagtccc cttaattgat
     5401 ggtcgaatca ttagttttga ctcattaaaa cgaaatggct ttgatgcaat cagcgttgat
     5461 ctaattgatg aaaaacactc tgttcgtgaa ttattgaatg aagaattaag ctgacacgca
     5521 ggccgtccga accctttcag acggtctgaa cattaatatt ccctaattaa tatgaccaat
     5581 aaacccgcca aacgcccggt caacggcgtt cttcttctcg acaaacccga aggcctttcc
     5641 agcaacaccg ccctgcaaaa aacgaggcac ttgttccgcg ccgaaaaagc cggacatacc
     5701 ggcgtgctcg atcctttggc aaccgggctt ttgcccgtct gcttcggcga agcgaccaag
     5761 ttcgcccaat atctgattaa tgccgacaaa gcctacaccg ccacactgaa actcggcgaa
     5821 gccagcagca cgggcgatgc cgaaggcgaa atcgttgcca ccgcccgcgc cgatatttcc
     5881 ttagccgaat ttcagacggc ctgccaagca ctgacaggca acatccgcca agtgccgccg
     5941 atgttttccg cgctcaagca cgaaggtaaa ccgctgtacg aatacgcccg caaaggcatc
     6001 gtcatcgaac gcaaggcgcg cgacattacc gtttacgcca tcgatattgc cgaatttgac
     6061 gcgcccaaag ccgtcatcga cgtacgttgc agcaaaggca cctatatccg caccctcagc
     6121 gaagacatcg ccaaacacat cggcacgttc gcccacctga ccgccctgcg ccgcaccgaa
     6181 accgccggct ttaccatcgc ccaaagccac acgctcgaag ccttggcaaa cttggacgaa
     6241 acagaacgcg acagcctgct gctgccctgc gacgtattgg ttgcccattt tccccaaacc
     6301 gttttaaacg attatgccgt ccatatgctc cgctgcgggc agcggccgcg tttcgaggaa
     6361 gacctacctt ccgatacgcc ggtacgcgtt tacacggaaa acggccgctt tgtcggtcta
     6421 gcggaatatc aaaaggaaat atgccgtctg aaagccttgc gcctgatgaa cacggcggca
     6481 tccgccgcct gaacgacggt taaaaatacg ggctgtgttt gaataatgtg ttgatatagt
     6541 ggattaacaa aaaccagtac agcgttgcct cgccttgccg tactatttgt actgtctgcg
     6601 gcttcgtcgc cttgtcctga tttttgttaa tccactatat ttccgcaaaa tcacgacaca
     6661 ctcggacacc cgccccgctt atcgcaactt tgcgaacgcc cccggaaaca gcaaagacat
     6721 caaataattg attttattag aatctatttg caaagccatt tgccgttaca caagaatggc
     6781 acattaaaat aactgatgag gatttataac gatgaagaca gacattcaaa ccgaattaac
     6841 ccaagcccta ctaccacacg aaaaagtatg ggcgaacgaa gaaaaaacca ttttagccaa
     6901 aaacatcctg ttggatttgg tggaaaaaac cgacccgacc attatcggtt tgttattgag
     6961 taatgatgag ttaaaacgcc atttctttgt ggaagtgaat ggtgtgctgg tgtttaaatt
     7021 gcaggatttc cgttttttct tggacaaaca cagcgtcaat aattcctaca caaaatacgc
     7081 caaccgcatt ggtttgacgg acagcaaccg ctttttgaaa gacagcagtg atattgtgtt
     7141 ggattttccg tttaaagatt gtgtgttaaa tggcggacaa agcaccgagg aaggcgaaga
     7201 aatttatttt aaacgcaata acagccagcc agccaattat acaccaaatt aacccgaaaa
     7261 agacaagaaa tcttttttaa tcaaacgctt gcttttgatg aaattgaccg actttttgac
     7321 gcaaaagcat tctcaaaatt ctctcgctat accgcagacg gcaaacaagc ggttggcgaa
     7381 atcaaacgcc attcagacgg cacaccggcc gaaaatctta ttatcaaagg caataatctg
     7441 attgccctgc attcgcttgc caagcagttt aaaggcaaag tgaagctgat ttatattgat
     7501 ccgccttatt attttgttaa aaagaaacaa caagatagtt ttggttataa cactaacttt
     7561 aaattatcta cttggcttac atttatgaaa aatcgtttat caattgccaa agaattatta
     7621 actgatgatg ggattattgt tatttccatt gatgatgacg gaaatgctta tttaaagatt
     7681 ttgttagatg aaatttttgg atttgaaaat tttatcggaa atttacccac tattatgaat
     7741 ttaaaaggta ataatgatga atatgctttt gcaggcactc acgaatacac actagtcttt
     7801 gctaaaaata aagataaatc tactttttat gaatttccaa tagatgaaga taatttttta
     7861 gagaaatggg aagaagatga aattgggttt tataaaaaag gagctccaat gcgagcaaca
     7921 ggaaccgaag ataaaagaga agatagacct gaaatgttct atcctttttt ggtaaaaaat
     7981 aacacagtta gtacaattac agatgaagaa ttttctcaaa tctataataa agatttagaa
     8041 gtattcaatg atgattttat tcaaaaacta aaagagaaat acgaaaatct tggatacaat
     8101 tttattttac ctattgctaa taaacaatgg gggcgttggc gttgggggta ttcaataaag
     8161 aataaggcaa ggctgcagac ggatattatt gttagccaat ctaaaaatgg aatttccttg
     8221 tataaaaaac aaagacctga attagatgat ttacctacaa aaaaacctaa aactattttt
     8281 tataaacctg aatacagtag tggtaatgga actgaacaga tgaaaaatct ttttggtgaa
     8341 aaggcattta aaaatcctaa accagaagaa cttatccaag attttattac catcacaacc
     8401 aacgaaaacg acatcgtcct agactaccat ttaggcagtg gcacaaccgc cgccgttgcc
     8461 cacaaaatga accgccaata tatcggtatt gaacaaatgg attatattga aacgcttgcc
     8521 gttgaacgct tgaaaaaagt gattgatggc gagcaaggcg gtatttccaa agccgtgaat
     8581 tggcaaggtg gtggcgagtt tgtttatgcc gaacttgccc catttaacga aaccgcaaaa
     8641 caacaaattt tggcttgcga agattcagac ggcatcaaaa cgctgtttga aggtttatgc
     8701 gaacgctatt tcttgaaata caacgtcagc gtaaatgaat ttagtcaaat cattcaagag
     8761 cctgaatttc aatccttggc attagacgaa caaaaacaaa tggtgcttga aatgttggat
     8821 ttaaatcaaa tgtatgtttc attatccgaa atggatgatg agcaatttgc aggttgctta
     8881 aatgatgacg ataaagcgtt aagccgtgaa ttctatcaat cagcaaaaaa tcaagcggag
     8941 aaaaaagatg gcgaataata aaacgttatt tgattgggtg gaagaccgca aatcaacgct
     9001 ggaagaaata gaacagacag attttttcgc gctgcctgaa tttgtagaac gcaatttaaa
     9061 atatccgttt tttgaatggc aaaaatcggc tttagaaaac tttgtgattt ttgaccgcgc
     9121 ttcaaaatta aaggatttcc ctgacatcaa aaaccgccct acccatttgc tgttcaatat
     9181 ggcaacaggt gctggcaaaa cgatgatgat ggcggcgttg attttgtatt attttgaaaa
     9241 aggttatcgg cattttttgt ttttcgtcaa tcaaaataat atcgtggata aaacggaaaa
     9301 taattttatt gacccggcgc acgcaaaatt tttatttacc gagaagattt tgcaaggtga
     9361 tacggtaatt cctattcgca aagtggaaat gttcagccca cattcagacg gcattgaaat
     9421 taaatttacc agcattcaaa aactgtataa cgatatccac accgagcgag aaaaccaaac
     9481 cacattggca gatttgcaca aattgaacct tgtgatgctg ggcgatgaag cacaccattt
     9541 aaacgcacaa accaaaggca aaaaacaaga cgagttggat ttggaagtgg aattaaaagc
     9601 caatgcaggc aatgccgaaa ttgaacgcaa aggctgggag catatggttt tggaattgtt
     9661 gctcaataaa aatggcaatc ccggccaaaa tgtgctgttg gaatttaccg ccacgctgcc
     9721 tgaaaatgct gacgtgcaac aaaaatatgc ggacaaaatc atcacaaaat ttggcttaaa
     9781 agagtttttg caaaaaggtt ataccaaaga aatcaatttg gtatccagta cgctgaataa
     9841 gaaagagcgg gtgttgcacg ctttattatt tgcttggtat cgccatcaaa tcgcgttgaa
     9901 atacggcatt gccaatttca agcctgtgat gttgtttaga agtaagacga ttgatgaatc
     9961 aaaagcggat tattcggcat ttttaaattg ggcagaaaat gtacaagcgg atgattttga
    10021 gtttttagat acgttttcag gcagcctgaa taatagcgac aacgccaacg aacaaggcaa
    10081 aacccgaacc gaacaagccc taaaatttat gcaggaaaat ggctttgagt ttgcacattt
    10141 ggcaaattgg gtgaaacaaa attatcaaaa acataatgtg attattacca actccgaaac
    10201 caacaaaaac aaaactgaaa aaaccgacag cgaaaccgaa aaattgctaa acaatttgga
    10261 agcggctgat aatccaatcc gtgccatttt tacggtagat agattaaccg aaggttggga
    10321 cgttctgaat ttgtttgata ttgtgcgttt gtatgaaggg caaaatggcg gtggttcaaa
    10381 taaaaaatca ggcaaaacgg ctgccgctac cgtatcggaa aagcagttga ttggtcgtgg
    10441 cgtgcgttat tttccatttg cgtttgaagg taaacagccg aataaacgca aatttgacaa
    10501 cgacatgcaa cacgaattgc gtatcttgga agaattgttt tattacacgc acgatgagca
    10561 atcccgctat atttcagaac tgaaaaatga gttgcgaaaa gacggttatt tgcctgaaaa
    10621 agacgatgat aaggtgttgg caacatttaa actcaaatct gaatttgccg ataataagga
    10681 ttttagagag ttgttaattt gggcgaataa aaaaatcccc aacccaaacg ccaaaagcaa
    10741 taatgcagac agcctgcaag ccaatccgca aacgctttca ttccaagttc acggtaatca
    10801 actgttgcag gaaacgcaat ttacagccga tgaaaatgat gaaacagccc gacaaatcgg
    10861 cacacaaaat aattttactc aaaccataaa aatgagtgaa atggagcggc acattttcaa
    10921 taaagcatta catatcaaag ggaaaaatag tcaatcttta ttccatttca accgtttgca
    10981 aagcaagctc gacattcaaa atcgcaatga attgcaaaat aacttgttga aagactggca
    11041 aattgaattt ttaggattag ggcaagacaa acaggttcgc ttggacgata aacttgcagg
    11101 ctgcctgaaa atcttggaaa tggttgaaaa acatctgaat gaaagtgata tgccgtttat
    11161 cggtacaaaa gaatttacac ctaaaaaatt atgggaaatt ttcggcacgc caaaacaaaa
    11221 atgggtcaaa aaagatgatg taaaaacgac tattgccacg caaaatgatt ggtatgtgat
    11281 gaataatttt gccggaacgg gtttggaaga agcgttaatt caatttattt cagcgcgttt
    11341 gggcgatttg aaatctaaat atgacgttca tttaatccgt aatgaagaag tgtttaaatt
    11401 gaataacttt gccgatggtg aaggatttat gccggacttt attttattgc tgaaagataa
    11461 acaaaaatct tcttccaatg gcgtgaagga ctttttgcat taccaaattt tcattgagcc
    11521 caaaggtgag catttagtgg aaactgatgg atggaaagaa gaatttttag aagccatcac
    11581 tgtggaatat gggatggata agatcctgca aaaagataca ccgcattatc gtttgatcgg
    11641 tttaccgttt tttactgatc atcagaaaaa tggacaattt acggagtcat tccctttagg
    11701 tgcggcatcg cttgaaaaaa tgaagtagtg cattgcaggc aaccccgttt gacaaaactt
    11761 cctttacaaa agggcgtttt gtcagatatt taatcaacac attattaaaa tacagccaaa
    11821 ttttaatgcc gtccgaaccc tgtgttcaga cggcatcgta tttttcagta tctaaaccgt
    11881 ttccctgccc caatctttgc ctctcaaaat cgaagcatcg acatcttgaa tatcgcggtg
    11941 tcccgtaaac gccatagata tatccatttc tttatacagg atttccagcg cacgggttac
    12001 gccttcttca ccatacgcac ccaaaccata cagaaacgcc cgacctatca ttgtaccttt
    12061 cgcacccaaa gcccacgcct tcaaaatatc ctgaccgctg cggatgccgc tgtccatcca
    12121 aacttcgatg tcgctgccga ctgcactaac gatgtcgggc aaggctttga tggcggacac
    12181 ggtatcgtcg agctggcggc cgccgtggtt ggaaacgacc aatgcgtccg caccgctttt
    12241 cgctgctttt tccgcatctt caggttccat aatgcctttg ataatcagct tgccacccca
    12301 caaatcttta atgcgtgcaa cgtcgtccca gctcaggcgc gggtcgaatt gttcggaagt
    12361 ccatgaagac agcgaggaca aatcgccgac gtttttggcg tgtccaacga tattgcggaa
    12421 cgtgcggcgt tccgtgttca gcattttcat acaccattcg ggcttggtcg ccagattgat
    12481 taaattggcg atggtcggtt tcggcggcgc ggacaggccg tttttgatgt ctttgtggcg
    12541 ttgccccaaa acctgcaaat cagcggtcaa taccaatgcc gaacatttgg catccttcgc
    12601 gcgcttaatc aggttttcca taaactcgcg gtcgcgcatc acataaagct gaaaccaaaa
    12661 tggtgcggaa gtgttctcgg caacgtcttc aatcgagcag atagacatcg tggacagcgt
    12721 aaacggaatg ccgaacttct ccgccgcccg cgccgccaaa atttcaccgt cggcgtgtgc
    12781 catacccgtg aaacccgtcg gcgcaatcgc caccggcatt ttcacatcct gcccgatcat
    12841 tttggtctcc aggcttcggc cttccatatt gaccaatacc ttttgtcgga agcggatgtc
    12901 tttgaaatcc gaagtgtttt cacggtaggt agtttctgtc cacgaacccg aatcgatgta
    12961 atcgtaaaac atacgcggca ttttgcgctt ggcaacgcgg cgcaagtctt cgatgcaggt
    13021 cattttgctc aaatcacgtt tcatttgtcg ccccctgaat acctgaataa ctttatatga
    13081 aatagataat gtatatcaat attgattata aggcaaatca tttcaacatt tactgcaccc
    13141 gccgccgctc ccacttcaag cggcataagg tttagaattc aaaaataata tattaaaatc
    13201 aaaatattaa aaatcaatca atctgccgat ttaaacagcc aatcgcgcaa tccgccccca
    13261 tacttgactg aaacactcag atattggaca attccgccca ctataaaaaa agccgacacg
    13321 ggcaaccacc accatgagac tgaccaccaa agggcgtttt gccgttaccg ctatgctgga
    13381 tttggcgatg aacgcgcaaa ccggcgccgt caaactcagt gccatcagcg aacgccaaaa
    13441 catatccctc tcctatctcg agcaattgtt cggcaaactc cgccgcgccg gacttgttga
    13501 aagcctgcgc gggcccggcg gcggctacat cctcgccgca ccggcggcac gcatcaacat
    13561 cgcccaaatc atcgccgccg ccgaagaccg gctggacgca acccaatgcg gcagcaaagc
    13621 caactgccac cacggcgcgc cctgcctgac gcacgacctt tgggagaatt taaacaaaac
    13681 catcaacgac tacctcggca gcgttaccct gcaaagcatc atcgaacaga aaaacaacgg
    13741 cgacggcagc cgcgtcgtcc aatttacaca catccattaa ataacacccg aaaaagaaag
    13801 agcaaaccat gaccgtcaaa acccccgttt acctcgacta cgccgccacc accaccgttg
    13861 acaaacgcgt tgccgaaaaa atgattccct atctgaccga aaccttcggc aaccccgcct
    13921 ccaacagcca cgcattcggc tgggaagcag aagaggccgt cgaaaaagcc cgcgccgaca
    13981 tcgctgccct gattaatgcc gaccccaaag aaatcgtctt caccagcggc gcgaccgagt
    14041 ccgacaacct cgccatcaaa ggcgcggcaa acttctacaa aaccaaaggc aaacacctca
    14101 ttaccgtcaa aaccgaacat aaagccgttt tagatacaat gcgcgaactc gaacgccaag
    14161 gttttgaagt aacctacctc ggcgttcaag aaaacggttt gattgatttg gaagaactca
    14221 aagccgccat ccgcgacgac accatcctcg tttccgtaat gtgggcaaac aacgaaatcg
    14281 gcgtggtgca agacattccc gccatcggcg aaatctgccg cgaacgcaaa atcgtcttcc
    14341 acgtcgatgc cgcacaagcc tgcggcaaag tgcctgtcga tgtcgaagcc gccaaaatcg
    14401 acctgctctc gatgtccgcg cacaaagtgt acggccccaa aggcatcggc gcgctgtacg
    14461 tccgccgcaa accccgcgtc cgcctcgaag cccaaatgca cggcggcggc cacgaacgcg
    14521 gcttccgcag cggcacattg ccgacccacc aaatcgtcgg tatgggcgaa gccttccgca
    14581 tcgccaaaga agaactcgaa caagatatgg cgcactaccg caaactgcgc gatattttcc
    14641 tcaaaggtat cgaaggcatc gaagaagtct atatcaacgg cgacctcgaa catcgcgtcc
    14701 cgaacaacct aaacgtcagc ttcaacttcg tcgaaggcga aagcctgatt atggcagtga
    14761 aagaactcgc cgtatccagc ggctccgcct gcacctccgc ctcgctcgaa cccagctacg
    14821 tcctgcgcgc attgggcaga aatgacgaat tggcgcactc gtccctgcgc atcaccttcg
    14881 gtcgcatgac cactgaagaa gaagtgcaat ttgccgccga actgattaaa tccaaaatcg
    14941 gcaaactgcg cgaactgtcg ccgttgtggg aaatgttcaa agacgggatt gatttgaact
    15001 cgattgaatg ggcggcgcat taaagcgtac caacatgcca tccgaacctt cagacggcat
    15061 tccaaaaaca aagcaatcaa gagaaaaata tgaacgaaca agatttagat ttggacaacc
    15121 tcgacaacct gcttgacgat ttcgacggcg ttaccgtgga aggcggcgtc gattcggaaa
    15181 acgacgacgg ctgtgaaggc ggagcgtgca aaatctaact caggccgtct gaaaaccggc
    15241 aaacaaacca cttaaatcat caaaacatta aggaaaccac atcatggcat acagcgataa
    15301 agtaatcgac cactacgaaa atccccgcaa cgtcggtact ttcgacaaaa acgacgaatc
    15361 tgtcggcacc ggcatggtcg gcgcaccggc ctgcggcgac gtgatgcgcc tgcaaatcaa
    15421 agtgaacgat gaaggcatca tcgaagatgc gaaattcaaa acttacggct gcggttccgc
    15481 catcgcttcg tccagcctga ttaccgagtg ggttaaaggc aaaagcctgg atgacgcgct
    15541 ggcaatcaaa aacagcgaaa tcgccgagga gttggaattg ccgccggtaa aaatccactg
    15601 ctccatcttg gctgaagatg cggtaaaagc ggccgttgcc gactaccgca aacgtcagga
    15661 aaacagataa agcccttcag acggcatcgt cccgcaatgc cgtccgaacc acccgccgct
    15721 tcaggtccgt ccggggcggt acaacaagga agaaatatga ttacccttac cgagaatgcc
    15781 gcaaaacaca tcaatgacta tctcgccaaa cgcggcaaag gcttgggcgt acgcttgggt
    15841 gtgaaaacca gcggctgctc ggggatggcg tacaaccttg aatttgtcga cgaagccgat
    15901 ggcgacgacc tgattttcga aggacacggc gcgcgcattt atatcgatcc gaaaagcctg
    15961 gtttatctgg atggcacgca agtcgattac accaaagaag gtttgcagga aggtttcaaa
    16021 tttgaaaacc ccaatgtcaa agactcctgc ggctgcggcg aaagcttcca tgtttaagac
    16081 ataaaaatgg cgggaccgta tcaaaaccgt cccgccattt tttcgcttcc tgcctgttgt
    16141 agctgccttt gccttttctt ttccgttcca ccttgtgtcg gaacaaatca gatttcacta
    16201 aggcttttaa agcattgtcg cgtattttgc ctttattgtg ctgcactttg ccgcccatat
    16261 tcagtccttt cgtttaagaa gcggcagatt ataaggcaaa aacagttttc tgccaaaatc
    16321 ttacatttat catcctacta tgtcccaata tttcaccctc ttccggattg aacccgcttt
    16381 cgatatcgac accgaaaact tggaacaaac ctaccgcgcc ttggccgccc gtttccatcc
    16441 cgataaattc gcttcagctt ccgcctttga gcaaaagcag gcagtgatga tgtcttccac
    16501 catcaacgat gcctaccgca ccttgaaaaa ccccatcgac cgcgccgcct acctgctgaa
    16561 aacatcgggc atcgatgccg acgcgccgga gcataccgct ttcgcccccg aattccttat
    16621 gcagcaaatg gaatggcgcg aaacgctgat ggaggcacgg gcaggcaacg accttgaatc
    16681 cttgaaaaat ctcgacaacg aaatccgcga cgaacaagaa aaactgttct gcggtctgaa
    16741 acagtcgttt gcccgacaag attacgacac agccgcacaa caagtccgcc aaggcaggtt
    16801 tctcgacaaa ctccgcaacg aaatttcctc ggcattataa tccgcaccgt gtttcagacg
    16861 gcgtaaccgc cgcaccgttc cgcgtcaaaa tatgctaaaa taagcaacaa ttttttgcca
    16921 tacgaaacat tgaaaccatg accgacgcaa ccatccgcca cgaccacaaa ttcgccctcg
    16981 aaaccctgcc ggtaagcctt gaagacgaaa tgcgcaaaag ctatctcgac tacgccatga
    17041 gcgtcattgt cgggcgcgcg ctgccggacg ttcgcgacgg tctcaagccg gtacaccgcc
    17101 gcgtactgta cgcgatgcac gagctgaaaa acaactggaa tgccgcctac aaaaaatcgg
    17161 cgcgcattgt cggcgacgtc atcggtaaat accaccccca cggcgatacc gccgtatacg
    17221 acaccatcgt ccgtatggcg caaaatttcg ctatgcgtta tgtgctgata gacggacagg
    17281 gcaacttcgg atcggtggac gggcttgccg ccgcagccat gcgctacacc gaaatccgca
    17341 tggcgaaaat ttcccacgaa atgctggcag acattgagga agaaaccgtc aatttcggcc
    17401 cgaactacga cggtagcgaa cacgagccgc ttgtactgcc gacccgtttc cccacactgc
    17461 tcgtcaacgg ctcgtccggc atcgccgtcg gcatggcgac caatatcccg ccgcacaacc
    17521 tttctgatac cgtcaatgcc tgcctgcgcc tgctcgatgc acccgacacc gaaatcgacg
    17581 aactgatcga cattatccaa gcccccgact tcccgaccgg ggcaaccatc tacggcttga
    17641 gcggcgtgcg cgaaggctat aaaacaggcc gcggccgcgt cgttatgcgc ggtaagaccc
    17701 atatcgaacc cataggcaga aacggcgaac gcgaagccat cgttatcgac gaaatcccct
    17761 atcaggtcaa caaagccaag ctggtcgaga aaatcggcga tttggttcga gaaaaaacac
    17821 tggaaggcat ttccgagctc cgcgacgaat ccgacaaatc cggtatgcgc gtcgttatcg
    17881 agctgaaacg caacgaaaat gccgaagtcg tcttaaacca actctacaaa ctgactccgc
    17941 tgcaagacag tttcggcatc aatatggtgg ttttggtcga cggacaaccg cgcctgttga
    18001 acctgaaaca gattctctcc gaattcctgc gccaccgccg cgaagtcgtt acccgacgta
    18061 cgcttttccg gctgaagaag gcacgccatg aagggcatat cgccgaaggc aaagcggtcg
    18121 cactgtccaa tatcgatgaa atcatcaagc tcatcaaaga atcgcccaac gcagccgagg
    18181 ccaaagacaa actgcttgcg cgtccttggc gcagcagcct cgttgaagaa atgctgacgc
    18241 gttccggtct ggatttggaa atgatgcgtc cggaaggatt ggctgcaaac atcggcttga
    18301 aagagcaagg ttattacctg agcgagattc aggcagatgc tattttacgc atgagcctgc
    18361 gaaacctgac cggcctcgat caagaagaaa ttgtcgaaag ctacaaaaac ctgatgggta
    18421 aaatcatcga ctttgtggat atcctctcca aacccgaacg cattacccaa atcatccgcg
    18481 acgaactgga agaaatcaaa accaactatg gcgacgaacg ccgcagcgaa atcaacccgt
    18541 tcggtggcga cattgccgat gaagacctga ttccgcaacg cgaaatggtc gttaccctga
    18601 cacatggcgg ctatatcaaa acccagccga ccaccgacta tcaggcgcag cgtcgcggcg
    18661 ggcgcggcaa acaggcggct gccaccaaag acgaagactt tatcgaaacc ctgtttgttg
    18721 ccaacacgca tgattatttg atgtgcttta ccaatttggg caagtgtcat tggattaagg
    18781 tttacaaact gcccgaaggc ggacgcaaca gccgcggccg tccgattaac aacgtcatcc
    18841 agttggaaga aggcgaaaaa gtcagcgcga ttctggcagt acgcgagttc cccgaagacc
    18901 aatacgtctt cttcgccacc gcgcagggaa tggtgaaaaa agtccaactt tccgccttta
    18961 aaaacgtccg cgcccaaggc attaaagcca tcgcgctcaa agaaggcgac tacctcgtcg
    19021 gcgctgcgca aacaggcggt gcggacgaca tcatgctgtt ctccaactta ggtaaagcca
    19081 tccgcttcaa cgaatactgg gaaaaatccg gcaacgacga agcggaagat gccgacatcg
    19141 aaaccgaaat ttcagacggc atcgaagatg aaaccgccga cagcgaaaac gcactgccga
    19201 gcggcaaaca cggtgttcgc ccgtccggtc gcggcagcgg cggtttgcgc ggtatgcgcc
    19261 tgcctgccga cggcaaaatc gtcagcctga ttaccttcgc ccctgaaacc gaagaaagcg
    19321 gtttgcaagt tttaaccgcc accgccaacg gatacggaaa acgcaccccg attgccgatt
    19381 acagccgcaa aaacaaaggc gggcaaggca atattgccat taacaccggc gagcgaaacg
    19441 gcgatttggt cgccgcaacc ttggtcggcg aaaccgacga tttgatgctg attaccagcg
    19501 gcggcgtact tatccgcacc aaagtcgaac aaatccgcga aaccggccgc gccgcagcag
    19561 gcgtgaaact gattaacttg gacgaaggcg aaaccttggt atcgctggaa cgtgttgccg
    19621 aagacgaatc cgaactctcc gacgcttctg taatttccaa tgtaaccgaa ccggaagtcg
    19681 agaactgaaa atcatctccc gatgccgtct gaagattcag acggcattta ttttatccct
    19741 catccgtcat ccagcttctc acaatatagc ggattatagt caattaaaaa caaggggctg
    19801 tcctagataa ctagggaaat tcaaattaag ttagagttgc ccctatgaga aaaagtcgtc
    19861 taagccggta taaacaaaat aaactcattg aactgtttgt cgcaggtgta actgcaagaa
    19921 cggcagcaga gttagtaggc gttaat
//
LOCUS       DAWFLT010000030        19601 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWFLT010000030 DAWFLT010000000
VERSION     DAWFLT010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19601
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.030"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(60..1118)
                     /gene="dinB"
                     /locus_tag="WGR74_001101"
     CDS             complement(60..1118)
                     /gene="dinB"
                     /locus_tag="WGR74_001101"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ0905530.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
     gene            complement(1262..2455)
                     /locus_tag="WGR74_001102"
     CDS             complement(1262..2455)
                     /locus_tag="WGR74_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0905531.1"
                     /translation="MYYYDEYSGSRTPVYVDVYELDEKGNKIQETNPDGTPAFTGFSG
                     TVPVWKTVKVADDHVPALYNYAKYLNTNKTHSLTAGTRFNVTGRLHLLGGLHYTRYET
                     SQTKDMPVRYGQPASDFQTASSIKADQDHYTAKMQGHKLTPYAGITYDLTPQQSIYGS
                     YTKIFKQQDNVDVSAKTVLPPLVGTNYEVGWKGAFLQGRLNASFALFYLEQKNRTVVD
                     FGYVPGAGGKQGSFQTVAKPIGKVVSRGAEFELSGELNEDWKVFAGYTYNKSRYKNAA
                     EVNAERLAKNTGADPYNFSNFTPVHIFRFGTSFHIPNTGLTVGGGVSAQSGTSSLYNI
                     RQGGYGLIDGFVRYELGKHAKLSLIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMK
                     LDWQF"
     gene            complement(2491..4350)
                     /locus_tag="WGR74_001103"
     CDS             complement(2491..4350)
                     /locus_tag="WGR74_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0905532.1"
                     /translation="MNQNHFSLKILTVMLLSAYGGSFADGVVPVSDGNTVSLDTVNVR
                     GSHALSGKTEKTRSYTIDRMSTATGMRIAGKDTPQSVSVITRSRLDDKAVHTLEEAMK
                     NTTGVNVVRDSGLQTRFLSRGFYIDQIGEDGITVNVAGRSGYTAKIDVSPSTDLAVYD
                     HIEVVRGATGLTQSNSEPGGTVNLIRKRPTASFKHTGELTADHRGSRRAVLDVSGSLN
                     KANTLRGRLVGAEEYKKSFKDRVWGRKHMVYGIAEADAGDSSVLTLGGMYQKSREVPD
                     FSGIILSCENQKTAPFSSTPACNRPLQLPRNTYLGEDWSRLSADKYNLFSGFKHVFDN
                     GWQLNAEVSYTKNESDAKVGQFFLKNEHAAGLSDEDAVGFLTEKNEVIPFEPKDKALE
                     KLKAYRDETAKEYRERKDDFVKNRFDNTAFEQYRSRRAAERKAGFDECMSAPFALDFI
                     CQGSWGDPGVDADKSEFVDKALAKEGIFNNAAQRFPNSLYDSSFNRKATANRRYSYMP
                     LRHTKDDRQWGIKLDLTGTYGLFGREHDFFVGYAYGDEKIRSEYLEIYERRHRVRPNT
                     GATHGVYAGSCQGSRTVICLLLWSGGIKNPIGRRTMKKATVPFMPKNAGMPRK"
     gene            4624..5403
                     /locus_tag="WGR74_001104"
     CDS             4624..5403
                     /locus_tag="WGR74_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0905533.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYRHSFHFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(5614..7026)
                     /locus_tag="WGR74_001105"
     CDS             complement(5614..7026)
                     /locus_tag="WGR74_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0905534.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFP
                     ALGSLHAYKIKAELVGRYHSGCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVT
                     DNPPTGLFANKKAARRALSSWAETYGLCPASAGILPDGYAEDEPCPVYVSGRCDKACG
                     RSDEQVLAFAHKLPILDWGKMHEVEITETDPLTGEKVVLHGMGGALEMGDGLWYFDKD
                     LPDAFKAKFKTDRKNIKEIG"
     gene            7117..7193
                     /locus_tag="WGR74_001106"
     tRNA            7117..7193
                     /locus_tag="WGR74_001106"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7151..7153,aa:Arg,seq:acg)
     gene            7211..7285
                     /locus_tag="WGR74_001107"
     tRNA            7211..7285
                     /locus_tag="WGR74_001107"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7244..7246,aa:Glu,seq:ttc)
     gene            7841..9016
                     /locus_tag="WGR74_001108"
     CDS             7841..9016
                     /locus_tag="WGR74_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ0905535.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(9226..9774)
                     /locus_tag="WGR74_001109"
     CDS             complement(9226..9774)
                     /locus_tag="WGR74_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ0905536.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(9777..11273)
                     /gene="ccoG"
                     /locus_tag="WGR74_001110"
     CDS             complement(9777..11273)
                     /gene="ccoG"
                     /locus_tag="WGR74_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ0905537.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            11801..13780
                     /gene="tkt"
                     /locus_tag="WGR74_001111"
     CDS             11801..13780
                     /gene="tkt"
                     /locus_tag="WGR74_001111"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ0905538.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQAALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVTWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGGYVISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVHVVSMPSTNVFDRQDTAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            13921..15309
                     /gene="fumC"
                     /locus_tag="WGR74_001112"
     CDS             13921..15309
                     /gene="fumC"
                     /locus_tag="WGR74_001112"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ0905539.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGKLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(15374..16297)
                     /locus_tag="WGR74_001113"
     CDS             complement(15374..16297)
                     /locus_tag="WGR74_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0905540.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKWRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAVGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAELAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALVVVGGAVTAAVGDRLFKRR"
     gene            16469..16609
                     /locus_tag="WGR74_001114"
     CDS             16469..16609
                     /locus_tag="WGR74_001114"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905541.1"
                     /translation="MPSENSGDVRQASLPQNARTAQSQRSAQRKGRQQAGSDAKKRNP
                     AP"
     gene            complement(16669..17193)
                     /locus_tag="WGR74_001115"
     CDS             complement(16669..17193)
                     /locus_tag="WGR74_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ0905542.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
     gene            complement(17197..18579)
                     /locus_tag="WGR74_001116"
     CDS             complement(17197..18579)
                     /locus_tag="WGR74_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0905543.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFILGIACIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGVAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQVNQEVLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            complement(18690..19313)
                     /locus_tag="WGR74_001117"
     CDS             complement(18690..19313)
                     /locus_tag="WGR74_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0905544.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
BASE COUNT         4825 a         5651 c         4694 g         4431 t
ORIGIN      
        1 ccatgccgtc tgaaaaccgt tcagacggca tattttttgg acggcgcgcg taaagcggtt
       61 tacgcccaca aatcctgctg ctggtttttc ggcacaagat gccccacgcc gataccgata
      121 aggcggaacg cgtcttccgt ctgcggcggg acgcgcgcca tcaacatttg cgcagcctgc
      181 agcagagctg cgcagtcggg caatacggag gaataagtca gtgtgcgcgt gatgatgcgg
      241 aaatcgtagg tcttcagctt gagcgttacg ctttgggctt cgacgttttt gcgcgtgatt
      301 tgccgccaca agtcttcggc aagatggggg aggtgtccgg cagcctgctc gagcggcagg
      361 tcttcgggca gggtaatttc tgtggagatt tggaggcgtt cgcgttcggc tttgacgggg
      421 cgttcgtccg taccgcgcac caaatcatag aggcggtatc cgtagcgtcc gaaatggttt
      481 aagagttcgc cgcgctcgaa acggcgcaag tcgcccgccg tccgcatacc cagcgactgc
      541 atttttttca gcgttacctt gcccacgccg gggattttgc ccaaaggcag ggtttccaaa
      601 aatgccatga ctttgtgcgg cggcaacaca aactgcccgt tcggcttgcg ccagtccgac
      661 gcgattttcg ccagaaattt gttcggcgcg atgcctgcgg atgcagtcaa acctgtttcc
      721 gcaaaaatgg cggcacggat ttctttggca acttcactgg cgtaagggat gtttttgaaa
      781 ttacgggtaa cgtcaagata ggcttcgtcc agcgacaagg gttcgattaa atcggtataa
      841 cgcctgaata cggcgtgaat ctgcgcggaa acctgacggt acaaatcgaa atgcggcggc
      901 acatacaccg cttgcggaca cagccttttc gccgttgcca ccgacatcgc ggaatgcagc
      961 ccgaactgcc gtgcctcata cgatgcggcg caaatcaccg aacgcgcgcc ctcccacgcg
     1021 acgaccaccg gccgcccttt caaatgcggc tgttcgcgca gctctaccga tgcgtagaat
     1081 gcgtccatgt cgatgtggat aattttgcgt gaagacatcg gctcttctga ggataaaagg
     1141 gatattctac tgccggcatc gggcaaattc caaatatacg ccccgataga cctgcctcca
     1201 taaaaatgcc gtctgaaaca taccctgttt cagacggcat ccgcaaaact acggttttca
     1261 attaaaactg ccaatccagt ttcatgctga cagtgcgcgg ctctccgtag aagttgtttg
     1321 cgccgcgcgt acggttgtag ttgttctcaa aataagtgcg tccgtttaag ttcgtaccga
     1381 tgaggctcaa tttggcgtgt ttgcccaatt cgtaacggac gaaaccgtct atcagcccgt
     1441 agccgccctg acggatgttg tacaggctgc ttgtgccgct ttgtgcggac acgccgccgc
     1501 cgacggtcag ccccgtattc ggtatatgga agctcgttcc gaaacggaat atgtgcacgg
     1561 gtgtgaaatt gctgaagttg tacgggtctg cgcctgtgtt tttggcgagg cgttcggcgt
     1621 tgacttcggc ggcgtttttg tagcggctct tgttgtaggt gtaacccgca aagactttcc
     1681 aatcttcgtt caactcaccc gacaactcga attccgcacc cctgctgacc actttgccta
     1741 tcggtttggc aacggtttgg aacgacccct gcttgccgcc tgctccggga acatagccaa
     1801 aatcgacgac cgtgcggttt ttctgttcga ggtaaaacaa tgcgaacgaa gcattcagcc
     1861 gtccttgcaa gaacgcgcct ttccagccta cctcatagtt tgtgccgacc aaaggcggta
     1921 aaacggtttt ggcactgaca tcgacattat cctgctgttt gaagattttg gtataacttc
     1981 cgtaaatact ctgttgcggt gtcaaatcat aggtaatgcc tgcatagggc gtcaatttat
     2041 gaccttgcat cttggccgta taatggtcct gatccgcctt aatgctcgat gccgtctgaa
     2101 aatcgcttgc cggctgccca tagcggacag gcatatcttt ggtttgcgag gtttcatagc
     2161 gcgtgtagtg cagcccgccc aaaagatgca gtcggccggt tacgttgaaa cgcgtgccgg
     2221 cagtcagcga atgggttttg ttggtgttga ggtatttggc gtagttatac agcgcaggaa
     2281 catggtcgtc ggccactttg acggttttcc aaaccggcac cgtaccggaa aaaccggtaa
     2341 aggcaggcgt gccgtcggga ttggtctcct gaatcttatt gcctttttca tccagttcat
     2401 atacatcgac atatactggc gtccggctgc cgctgtattc gtcatagtaa tacacctgct
     2461 tgccttcggc atcgagcttg ggctcggttt ttattttctt ggcattcctg cattcttcgg
     2521 cataaacggt acggttgcct ttttcatcgt acgcctgcca atcgggttct ttatgccccc
     2581 tgaccaaagg agaagacaaa tcaccgtccg gctcccctga caacttcccg catacacgcc
     2641 gtgcgttgcc cctgtattcg gacgtactct gtggcggcgt tcgtagattt ccagatattc
     2701 ggaacgtatc ttttcatcgc cgtaggcata gccgacaaag aaatcatgct cccgcccgaa
     2761 cagcccatat gtgccggtca ggtcaagttt aattccccat tggcggtcgt ctttggtatg
     2821 ccgcaacggc atataactgt atcgtcggtt ggcggtagcc ttccgattaa aggaagagtc
     2881 atacaggctg tttggaaaac gttgtgccgc attattaaag atgccttcct tcgcaagggc
     2941 tttatcgaca aattccgact tgtcggcatc aacacccgga tccccccaag aaccttgaca
     3001 gataaagtcc agcgcaaaag gggcactcat acactcgtca aaaccggctt tgcgttctgc
     3061 ggcacggcgg ctgcggtact gctcgaaagc agtattatcg aaacggtttt taacaaaatc
     3121 gtctttgcgc tcacggtatt ccttggcggt ttcgtcacga tatgctttca gtttctccaa
     3181 tgccttatct ttcggctcga acgggatgac ttcgtttttt tcggtcaaaa agcctaccgc
     3241 atcctcatct gacaaacccg ccgcatgttc gtttttcaga aaaaactgcc ccaccttcgc
     3301 atcggattca ttcttggtat aagacacttc ggcattgagc tgccaaccgt tgtcaaacac
     3361 atgtttgaaa cctgagaaaa ggttgtattt gtcagcactc aaccgcgacc aatcctcccc
     3421 caaataagtg ttgcgcggca gttgcaaagg ccggttgcag gcaggcgttg aactgaacgg
     3481 ggcagttttc tgattttcac aggacaaaat aatgcccgaa aaatcaggaa cctccctact
     3541 cttctgatac atgccgccca aagtaagcac actgctgtcg cccgcatcgg cttcggcaat
     3601 gccgtaaacc atatgtttcc tgccccaaac gcggtcttta aacgattttt tatactcttc
     3661 cgcacccacc aaccttccgc gtaaggtatt cgccttattc aggctgcctg aaacatccaa
     3721 cactgcacgc cggctgccgc gatggtcggc ggtcagctct ccggtatgtt tgaaagaagc
     3781 ggtaggtcgc ttacggatca aattgacggt tccacccggc tctgaattgg attgggtcaa
     3841 ccccgttgca ccccgtacaa cttcaatatg gtcataaacc gccaaatcgg tactcggaga
     3901 cacgtcgatt ttcgccgtat atcccgaacg gcctgcaaca ttgacggtaa taccgtcttc
     3961 accaatctga tcaatataga aaccgcgtga caaaaaccgc gtctgcaagc ctgaatcgcg
     4021 cacaacgttg acacccgtcg tgtttttcat tgcctcttca agcgtatgca ccgccttatc
     4081 gtcaaggcgg ctgcgcgtga tgacgctgac cgactgcggc gtatccttgc ccgcaatcct
     4141 catacctgtg gcggtggaca tccgatctat cgtataagaa cgggtcttct cggtcttgcc
     4201 cgacagagca tgagagccgc gtacattgac cgtatccaga ctgacggtat tgccgtctga
     4261 aacaggcaca acaccgtctg caaaagaacc accgtaagcc gataacagca taacggtcag
     4321 aattttaagt gaaaaatgat tttgattcat agagacctct gtaatatgca agtgtgcaaa
     4381 tcgtccaaag gccctcacaa ctgttttgat tttttatatt aattgaaaaa aagtaattct
     4441 caattaaatt tatagatggg attgtattcc cattttgaca aaaaacaaac tgctccttac
     4501 cgtttatttc aaaaaacgat aatattgtat tgaaaaatat ccgaatttaa atacagatcg
     4561 tcaatgcgga aaaaaacacc caaattggct ataatcccga caaacacact caaggacaac
     4621 aacatggcag cctcgcccga agcaaaattc actgaagaaa agattttgtg ggtcaaacac
     4681 cacacgccga aactcatcac tttcgccatc agccgtcccg aatcctaccg ttttaaagcc
     4741 ggacagttct cccgactcgg tttctacgaa ggggaaggct ttatttggcg tgcctactcc
     4801 atcgtttccg cagaatatgc agacacgctc gaatattttg ccgtactcat ccaagacggc
     4861 cccatgtcgg cccgtttcgc caaaatgcaa cagggcgaca ccatcctgct cgataaaaat
     4921 gccaccggct tcctcctgcc cgaacgcttc cccgacggca aggatttggt gatgctctgc
     4981 accggctccg gcatcgcccc cttcctttcc attctcgaac aacctgaaat ccgacagcgt
     5041 ttcgataccg tcaacctgat acattccgta tcttttcccg aagaattgat tttcaacgac
     5101 cggctcgccg cattgtccga acatcccctg gtaggcgaat acagacactc gttccatttc
     5161 gtccctgtta ccacccgtgc cgccaacccc tcgggcttaa gcggaaaacg cattccggaa
     5221 ctcttaaaaa acagcagcat cgaacaggcg ctgcatacca agttcacccc ggaatccaca
     5281 cggtttatga tttgcggcaa cccggaaatg gtcaaagata ctttccaaac gctgcttgac
     5341 atgggttacg ccatgcaccg caaccgcatt cccggtcaaa tcatgatgga aaacggcttc
     5401 taaaaaccac cctgcttgtc cgatgccttc ggatggacgg tcaaaccgac acggcacgaa
     5461 aaccgcgtcg gcaaaaatgc cgtctgaaaa aattcagacg gcatcttcgg acacattacc
     5521 tgcaaacggc aacacattcc aacccaaacc gattaggcaa tcaatacgaa cggctgttta
     5581 catacttacc ggctttcatc aaccggtatc gatttaaccg atttccttaa tatttttcct
     5641 gtccgtttta aacttcgcct taaacgcatc cggtaaatct ttatcgaaat accaaagccc
     5701 gtcacccatt tccaatgcgc cgcccattcc atgcagaaca actttttccc ccgttaaagg
     5761 atcagtttcg gtaatttcta cctcatgcat ttttccccaa tccaaaatag gcaacttgtg
     5821 tgcaaacgcc aaaacctgtt cgtcagaacg gccgcacgcc ttatcgcatc ggcctgaaac
     5881 atatacggga caaggctcat cctctgcata accgtccgga aggataccgg ctgaggcagg
     5941 acacaatccg tatgtttccg cccacgacga caaagcccgt ctcgccgcct ttttattggc
     6001 aaataatccg gtaggcggat tatccgtcac accgtttttc aaagccgctg ttttcgcatt
     6061 caacatgccg tctgaacctt tttcaaacct gacggtcgta aatgttttaa gcagattttt
     6121 ggcagataca taacaacccg aatgataacg tccgaccaat tccgctttaa tcttatatgc
     6181 atgcaggctg cccaatgcag gaaaaaaacg gacttcctcc gtattgcacc aatcaaacgg
     6241 ggcttttccg gaatccaata aagccgaaac ctcgctatat acccgttcaa acgtaccgat
     6301 gtaatttact ttccctccgc cgtcgaaaca agccagcacc cccataccgt caggcaaacc
     6361 gtacaactgt tccctcaacc gttcgggcag cgcggcaggc agcggtttcg gattcatcaa
     6421 acggaaacac tgcctgatcc atgcctcaac cccatgttcc gacaaactgt attccaaata
     6481 atcacacaat gccgacacat ccgccatcgc acgatgcctg tcttctacaa caatccccaa
     6541 cctttcgatg atactgtcca ggctgtgctt gtaaaattgc ggatacagac accgggacag
     6601 ttgcacactg cacaaagcag gcgatgaaaa cccgatgccc gcacgatgaa actcatgctt
     6661 taaaaacgta tagtcgaaac ggctgttatg tgcaaccagc acacaaccct tcaataccga
     6721 aaacaactcg ccggcaatct ctgcaaaaac aggcgcatcg gcaaccatgc cgtctgaaat
     6781 ccccgtcagc cccgccacaa actgcggaat cggtttttga ggattaacca accactcatg
     6841 cctcaccacc cttccctgct caaacttgac caaagccact tcggttaccc tgtcttcata
     6901 cagattgccg cccgtcgatt ccaaatcgac cacggcaaca ggcattccaa accgtaaaaa
     6961 taccttttcc agcaagggcc agcgggaagc aacaatcatt ttattctctt taaattcaaa
     7021 caacaaaccg atattttaca cttttaaggc atttcatcca acaaaacaat tgacagaatc
     7081 cgatgattac cctaaaattc aaatctttct tgcagtgcac ccgtagctca gttggataga
     7141 gtatctggct acgaaccaga gggtcgggcg ttcgaatcgc tccgggtgcg ccagtaagaa
     7201 aatacaatat gcgcccatcg tctagcggtt aggacatcgc cctttcacgg cggtaaccgg
     7261 ggttcgattc cccgtgggcg tgccaaattc ccaatccctg agattatccc tcggggattt
     7321 cttattacct cgacaactcg ttaccatatc tttacccccc ttcatcagac tctcagacgt
     7381 aatcgaatca tattcaaacc tttgccatgc aaaccgatat cacgtaaccg gatgcggtgt
     7441 ccgtccaaca ttttacccga ttgaaacgcc tgatatattg caccccatca acgtggcatt
     7501 acttttctta acaatcccct ttgacagcaa ctgactaggg cttttttatg ccatcatcaa
     7561 atttatagtg gattaacttt aaaccagtac ggcgttgcct cgcctttccg tactatttgt
     7621 actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa ttcactataa tattttctct
     7681 cccgattgaa acaggcgtaa cagaatatgc ccgaagctcc ggctgctttc ttgtttaccg
     7741 ccgcgatatt tagagtataa taccaaattt gagcaatagt tctaaaacag ttagaaccat
     7801 ttttcatgag cctgactgat tcgtacactc ggagaaactg atgcagaata tttttgaccc
     7861 tttggttatt cgtggaaaat cccttacccc catcgtgcaa ggcggtatgg gggtcggtgt
     7921 ttccgcatcg ggtttatcca gcgcggtggc acgtgaaaac ggtatcggaa cgattgccag
     7981 tgtggatttg cgccaccttc acgaagacct actcgccgaa tcacaaatca atccgagtga
     8041 agagaaatat acatctttga actgtaccgc attagacagg gaaatccaaa aagccaaaag
     8101 cgcttcagag ggaaaaggac tgattgcggt caacgtgatg aaggcggtca aagaccacgc
     8161 cgcgtatgtc cgccaggctt gcgaatcagg ggcggatgcg gttgtaatgg gtgccggtct
     8221 gcctttagac ctgccggaaa tgaccgaggg ctatcataaa gatgtcgcgc tgctgccgat
     8281 tctgtccgaa tcgcgcggta ttaatatcgt cttgaaacgt tggatgaaaa aaggcatatt
     8341 gcccgatgcg attgtagtcg aacatcctgc ccacgcggcc ggacatttgg gtgcatcaac
     8401 cgttgaaggc gtaaacgatg ccaagttcga cttcaaacgc gtgattgagg aaacgtttga
     8461 agttttcaaa agtttagggc tggaaagcga aaaaatcccg cttattcttg cgggaggcat
     8521 ggcaaatttt gaaaaagtca aaaccgccct aaagaactgg ggagcatccg ccgttcaaat
     8581 cggtacggct tttgccgtta ccgaagaagg agatgcacac cttaacttca aaaaaacgct
     8641 cgccggtgcg gaaactgaaa aagtagtcga atttatgtct gttgccggtt tgccggcgcg
     8701 cggtgtccgc accaaattcc tagacagcta catcaagcgt gaaagcaaac ttcagacaaa
     8761 cgccaaagcc gacccgcgcc gctgtaccca aggtttaaac tgcctaacca gttgcggtct
     8821 gcgcgacggg ctttccaaag ccggacaatt ctgcattgat atccagcttg ccgccgcatt
     8881 ccgtggagaa gtagataaag gcctgttctt cagaggtaaa gacccgctgc ccttcggcaa
     8941 tgccatccgc accgtccgcg agacgataca atatctgctg acggggagcg aacctgttgc
     9001 aacgctcgga cgctgacatc aggtaggatt tgatttgcaa acaaaatgcc gtctgaagcc
     9061 cttcagacgg catttttcag gctgcatttc ggaatggtgt taaaaaataa acgggataag
     9121 atacatttat tttcgtccga taaatcagac catcacgccg aataatcaaa caatgcctgc
     9181 ccgacattgc atctggcaaa ccaatgcaat gaaaacacgg cttttttatt tgcttccggc
     9241 attattgaaa agtttatcca tcggggtcag atccaccgca ttgccttggc tggtaatcca
     9301 tttgttctcg acgcgccaca cgcctgccgc gtcctccacg cgcacatacc agtggttggt
     9361 cggcgaaagg gttttgaaca ccgcctcata ttccgccctg ccgttctgcg cgctgccgac
     9421 gggcttgagg gcgacggttt gatcgtccgc cttgcgggtc gggtgcatca gcagcaggtt
     9481 caaaggctgt ttgccgtcaa actcgccgcc gacaaacact tttgccgcat tcatatcggg
     9541 ggaaatgaga acctgcaccc cgatatgccg tctgacagcc tcttcatccc gatgaagctg
     9601 gatgtcgata tgtttgccgt ctttataata atcgtccgta accaaatctg tcgcgtgctg
     9661 ctgtgcgaca aaaaacatag cgacgctggc gatgacgaca aaaatcggcc ccgccatcaa
     9721 gatccacggc cagacgtgtt tgtaccaagg tttgattgga gtgttttgag acacggttat
     9781 tctccgataa aggttgcatc ttcttccaag acgaccggct tgccgtcggg cgcgccgctt
     9841 tcgcggtatt ggaaggtaaa ttcgataggg tggctgcctt tgtccgcgta ttccggaatg
     9901 gtggacactt ggacgggaag ggttaccgtt tcgcgcgggg caaccttgat accgccttcg
     9961 ggcagcccgg tcagggcgat ttcgtcaaag cctttgacac ttgcggtaat gagctgttct
    10021 ttttcacttt tgttgatgat acgcaggctg tatgcgtttt ccagccagcc tttggcgttt
    10081 tcgcgcacca gtacgccacg gtctttcaaa atatcgacct cgaccatttt gcgcgtggac
    10141 aaaccggcca ggaaggcaat gataactaac gccaacaccg cgccgtaacc tgccacgcgc
    10201 ggtctgagca gccgtttttt aatgtctttt tcagaatatt cgtgttccag cgcgctttcg
    10261 gtcgtataac ggattaatcc gcgcggatag cccattttgt ccataatctc atcgcacgcg
    10321 tcgatacagg cggcgcagcc gatacattgg tattgcagac cgttgcggat gtcgatgccg
    10381 acggggcaga cttggacgca catcgcacag ttgatgcagt cgcccaaacc cgcctcttcc
    10441 ttattgaccg ttttcttgcg cgcgccgcgc ggttcgccgc gttccgcgtc ataagaaaca
    10501 atcagcgtgt ccttgtcgaa catcgcgctt tggaaacgtg catacggaca catatgcagg
    10561 catacttttt cacgcataat gtgggcgaag aagaaggtca taaagccgta aaacgctgcg
    10621 gcaaacatcg cgccgccacc tgctgctcca gtgaataaat cgggaacgaa ctggcggata
    10681 gggacaaacc agcctgcaaa cgtgatgccc gtccacgcgc agacaaggaa aatcagcagg
    10741 tatttggtgg ctttgatgcg gattttagtg aaattccacg gcgatttttc cagtttcagc
    10801 cgtttgtttc tatcgccttc gaccaggttg tcaatccaca gcataatttc ggtgtaaacc
    10861 gtttgcgggc aggaatagcc gcaccacagt cgccctgcaa tcgtcgtcca ccaaaacagc
    10921 ccgaaggcgc aaatcatcag cagcaaggca aggtaaatca aatcgcccac ccccaacgac
    10981 aatccgaaaa tgaagaaatg ccgttcgggg atattgaaaa cgacggcctg cctgccgctc
    11041 cagttgaacc acggaatgac gtaaaacaca aactgcgtcg ccaatacggc ggcgatacgc
    11101 agtttggcga accgtccttc cgcctttttg ggatggatgc gttcgccttc gggatggatt
    11161 tgaatcacgc tggctcgcgg atcgaatgtt ttttttgctt tcggcgcggc ttttgtttgt
    11221 tcggacgtgc cgattccgga tgccggactg ccggcttggt tttccgtggt cattctgcat
    11281 tccttagatt tttgattgat ggtttgcccg ttaccgccgc cgtttgcttt tcagacgtca
    11341 tttttcttgt tttttaaggc gttgtgtttc aagttttgag aaaatccgtt tttcccaaaa
    11401 tatatttccg ctattgtaca actttatgcg ccgtccggat gtatggggcg gatacatttc
    11461 ccatccgcat caaaacgcct ggattttacc ttaccgcccg aacaaaatcc gaatacggtt
    11521 aaaaaaaaag actaaaaaaa ccgacacccc catatcggca gaaccgacgg cgcaagctca
    11581 taaacaaacg ctatcgacaa tccggcacac aatctataac tttttatttc aaaaggaata
    11641 atggcaggct tcgcccgcaa atcgaaaatc cttccccgcc tgtcccctgc cgccgccttc
    11701 ccacgcgtcc gcccttttct tgaaagcata agcgaatcgg gcgataatca acgctttccg
    11761 attatccact tatctgaaac accagcaagg aaaatacaaa atgtctcaac tggcaaacgc
    11821 aatccgcttc ctctcggccg atgccgttca aaaagccaat tccggccacc ccggcgcgcc
    11881 tatgggtatg gcggaaatgg cggaaacatt gtggacgaaa ttcctcaatc acaaccccgc
    11941 caaccccaaa ttctacaacc gcgaccgctt cgtcctctcc aacggccacg cgtctatgct
    12001 gttgtacagc ctgctgcacc tgaccggcta caatctgagc attgaagact tgaaaaactt
    12061 ccgccagctg cacagcaaaa cccccggcca ccccgaatac ggctacaccg acggcgtgga
    12121 aaccacgacc ggcccgttgg ggcaagggat tgccaacgcg gtgggtatgg cattggcaga
    12181 aaaaatcctt gccgccgaat ttaataaaga cggtttgaac atcgtcgatc attacaccta
    12241 cgtctttatg ggcgacggct gtctgatgga aggcgtatcg cacgaagcct gttcgctcgc
    12301 cggcaccttg ggcttgggca aactgattgt tttgtatgat gacaacaata tttccattga
    12361 tggtaaagtg gacggctggt ttaccgaaaa catcccgcaa cgctttgaaa gctacggctg
    12421 gcacgtcgtt cccaatgtaa acggtcatga caccgccgcc attcaagccg ccatcgaagc
    12481 cgcacgtgcc gaaaccggca aaccgtccat catctgctgc aaaaccttaa tcggcaaagg
    12541 cagtgccaac aaagaaggca gccacaaaac ccacggcgca cctttgggcg cggacgaaat
    12601 cgaagccacg cgcaaacatt tgggctggac ttaccccgcc tttgaaatcc cgcaagaaat
    12661 ttacgatgcg tggaatgcca aagaacaagg cgcgaaactg gaagccgact ggaacgaact
    12721 gttcgcgcaa tatcaagcca aatatcctgc cgaagccgca gaatttgtgc gccgtatgga
    12781 taaaaagctg ccggacaatt tcgatgaata cgttcaagcc gcattgaaag aagtgtgcgc
    12841 caaagccgaa accatcgcca cccgcaaagc cagccaaaac agcatcgaaa tcttggcaaa
    12901 agagctgccc gaactggtgg gcggctctgc cgacctgacc ccgtccaacc tgaccgactg
    12961 gtcaaacagc gtttccgtta cccgcgacaa aggcggcaac tacatccact acggcgtgcg
    13021 cgagttcggt atgggcgcga ttatgaacgg tttggtattg cacggcggcg taaaaccctt
    13081 cggcgcgact ttcctgatgt tcagcgaata cgagcgcaac gccctgcgta tggctgcgtt
    13141 gatgaaaatc aaccctgtat ttgtgttcac ccacgattcc atcggtttgg gcgaagacgg
    13201 cccgacccac cagccgattg agcaaaccgc caccctgcgc ctgattccga atatggacgt
    13261 atggcggccg tgcgacaccg ccgaatcctt ggtcacttgg gcagaagccg tcaaagccgc
    13321 cgatcacccg tcctgcctga ttttcagccg tcaaaacctg aaattccaag cgcgcagcga
    13381 gcaacaactg aacgacatca aacgcggcgg ctacgtcatc agcgaagcac aaggcaacgc
    13441 ccaagccgtc atcattgcca ccggttccga agtcgagctg gctttggaag cgcaaaaagc
    13501 cctcgccgcg caaaacatcg ccgtgcacgt cgtttccatg ccgtccacca acgtattcga
    13561 ccgccaagac accgcctatc aagccgccgt cctgcccgaa agcctgccgc gcatcgccgt
    13621 agaagccgga cacgccgacg gctggtacaa atacgtcgga ctgaacggcg cagtcgtcgg
    13681 catcaaccgc ttcggcgaat ccgcccctgc cgatttactc ttcaaagcat tcggctttac
    13741 cgtggacaat gtggtcgata cagtgaaatc agtgctgtaa ccccactcct aaacaaatgc
    13801 cgtctgaacg cttttcagac ggcatttttt tgccctcccg ccttgatgct tcctcattcc
    13861 accaatctcc attataatat ttgcgaatca ctcttattca catttcaaaa ggagaaacgt
    13921 atgaacactc gcaccgaaca cgacaccatg ggcaatgtcg aagtcccgtc cgaagcctat
    13981 tggggcgcgc agacccagcg cagccgcaac aatttcaaaa tcggcggcga aaccctgccg
    14041 cagccgctga tttatgcttt ggcgttggtg aaaaaagccg ctgccgcgac caatgtttcc
    14101 ctcggcagga ttaagccgga acaggcggat ttgattacgc aggcggcgga tgatgtgttg
    14161 aacggcaagc tcgacggaca gtttccttta gtggtttggc agaccggctc cggcacgcag
    14221 tccaatatga acatgaacga agttctggcg aaccgcgcca acgaaatcgc cggtacgggt
    14281 ttggcggcgt atcagcccgt ccatcccaac gaccatgtga accacgcgca atcgaccaac
    14341 gacgcattcc cgaccgctat ccacgttgcc gccgcgattg aaatcaaccg ccacctcatc
    14401 cccgccgtaa aagccctgcg cgacaccttg gacaaaaaag cccaagcctt cgcccctatc
    14461 gtcaaaatcg gccgcaccca cttgcaggac gcgacgccgc tgactttagg ccaagaattt
    14521 tccggctacg tttcccagct cgaccacggc ttaggccgtc tgaacgacgc gcttaaagac
    14581 ttgtatgaac ttgctttggg cggtacggcg gtcggcacgg gtttgaacag ccatcccgaa
    14641 tacgccgaaa aagccgccgc caaactcgcc gaattgtccg gcttgccgtt tgtcagcgcg
    14701 ccgaacaaat ttgaagcctt gggcggacgc gatgccgccg ttgccgcttc gggcgcattg
    14761 aaaacgctgg cggcaagcct gaataaaatc gccaacgaca tccgctggct ggcaagcggc
    14821 ccgcgctgcg gtttgggcga aatcaaaatc cccgaaaacg agccgggttc gtccatcatg
    14881 ccgggcaaag tcaacccgac ccaatgcgaa gcgatgacca tggtgtgctg ccaagtgttc
    14941 ggcaacgacg ttaccatcgg tatggcgggc gcgtcgggca atttcgagct gaacgtctat
    15001 atgcccgtca tcgcctacaa cctcttgcaa tccatccgcc tgttgggcga cgcgtgcaac
    15061 agcttcaacg aacactgcgc cgtcggcatt gaacccgtac cggaaaaaat cgactatttc
    15121 ctgcaccatt ccctgatgct cgttaccgcg ttaaaccgca aaatcggtta cgaaaacgcc
    15181 gccaaagtcg ccaaaaccgc ctacaaaaac aacaaatcgt tgcgcgaaac cgccgttgag
    15241 ttgggcttgc tgacgggcga agaatttgac gaactggtcg ttcctgccga tatggttcat
    15301 ccgcgctaat ctttccctca aataaaatgc cgtctgaaac ctcgttcgga cggcattttc
    15361 cgttgcctgc gaactagcgg cgtttgaaca gcctgtcccc caccgccgcc gtaaccgcac
    15421 ccccgaccac gaccagtgcg ccggcataac ccaaaccgtt catatccggc gcggcaaaag
    15481 tatcaggcat cacataatgc ccgagcaaag aaaatattac ggtaaacacg gggagcaagg
    15541 ttgttaccgc gctgactttg gaagcctccc aatgtttcaa cgcctcgccg aacgagccgt
    15601 aaccgattaa cgtattcaag cagcaatacg caaaacaaac ccacgccaac gtaccgtcca
    15661 aacttccgat gtgtgccagt tcggcaaacg gcaggaacac ggcggcactt gccgcataaa
    15721 tcaacagcag aatctgttgc ggcccgaatt gcgccgacag cagcttttgc gccacggcat
    15781 aacacaccca tgccatactg cctaccgcac acagcaacac gcccttcgca tacgcgccca
    15841 aacccgacaa ctcgccgaat ttatcgttaa aaaacataag caaaccggca agcagcaaaa
    15901 ccaagccgat tttctgagcg gcagtcatcc ggtctttaaa caccaacaca ccgacaacaa
    15961 tcatcgtaaa cggcgaaatc tgccacaaaa cctgcgtcgt ggtcggcgaa atataatgca
    16021 gcccttgggc aatcagcaca aagtttgccg aaatgcccgc cacgccgagc agcagcagcc
    16081 tgaatgagca ccaagaaaaa tcccgccact tcggcagccg cccgcccaat gccagcaaaa
    16141 caaacaatac cgccgccgcc acggtaaaac gcacccacac cagcgtcggc gcatcgacaa
    16201 acttcaatac ctgccgcacg gcaatcggca gcgttcccca cgtcatcgcc gccaaaagtg
    16261 ccaacgcgaa gcctaggagc ggcctttggt tttccatcct gattttccta tttttaaaca
    16321 accgtattgc cggacgatgc cggtttgccg catcgggcaa tgatggttca agcgtttgac
    16381 gtttgattcc aaccctttga tttcaaacaa accggctgaa gcccgactat tgcttcgcgc
    16441 tatttggaaa caccgcctga attttaaaat gccgtctgaa aacagcgggg acgtgcggca
    16501 ggcatcactg cctcaaaacg cccgaacggc gcaatcgcaa cgttccgccc aacgcaaggg
    16561 gcggcaacaa gccggctcgg atgcaaaaaa gagaaaccct gccccgtaag gtttaaggtt
    16621 tctccgtcct ttatgatttc cctctgcgag gatgtccggc cgtaaaattc agaacgggat
    16681 gtcgtcgtca atatcctcga ccggggcggc ggcaggcacg ggttggcggc gcggcgcggc
    16741 tggtgcttct tggggatggg acggcgcgtc ggaggcgggc tgccggcttt gctgtgcggg
    16801 gcgttggtaa gcctcctgac tctgaccgta accttcctcg taaggcgcac cgccgctgtt
    16861 ttcattgcgc ccgcccaaca ttttcatttc gttggcgaca atatcgtaag cggtgcgttc
    16921 gatgccgtct ttgccttggt atttgcggct ttggattctg ccttccagat aaaccagccc
    16981 gccttttttg aggtattgcc cggcaatttc cgccagtttg cggtacatcg tgatgttgtg
    17041 ccactcagta cgctctacac gttggccgtt gcggtcgttc caagtttcgc tggtggcgac
    17101 gctgaaatta cacaccgcct cgccgttggg catatagcgc acttcgggat cgcgtccgag
    17161 gcggccgata aggataactt tgttcaatga cattttttaa actcctgtga tgattttttc
    17221 agcggcagcc tgatcgaaac ccttctgcaa cactttgaga tagacggtct gcccgtcgaa
    17281 actgaaaccg atgtcttcca caccctcaag ctccgacaag gcgcggtata acacttcctg
    17341 attgacctgc cacacgccgc cgacagggta actgaggttt ttgacgggct tgggcgcagg
    17401 cgataaaacg gcaatcacca gccacagcag catcaatatg ctgcaaaagg caaacacgcc
    17461 ggaaaagccg tatttttgaa acagcaaacc gcctgccacg ccgccggcaa acagtccgag
    17521 cgactgcatc gtgttgtaca cgcccatcgc cgtacccttc agatcggacg gcgcgatttt
    17581 ggaaaccata gacggcaggc tcgcttccaa cacattaaaa ccgataaagt aaacaaccaa
    17641 ataagcggta atcaagccta ccgagcgcat accggacagc aaaccgagct gtgccgccgc
    17701 gatgcaggcg atgcccaaga taaaaacctg tttgagcttg ttgcgcgtct cgccgacgat
    17761 aatcagcgga accatcacca ccaagcccgt aatggtcgag ggcagataga ctttccaatg
    17821 ctggattttt tccaaaccga gctgcgtcat cgcaaaaggc agcgcggtaa acaatgccat
    17881 ttgcgcggcg tgcagggcga aaatgccgaa atcgagcgtc agcagcctac ggtttttcaa
    17941 aacttcgcct atgcgcgaag gctgcgcctg cgtatcttcg tgcagcttgg aaacctccgg
    18001 gtcgggagtc atccacgcca ccacgccgat actggcaacg gtcaaaatac cggtcagcag
    18061 aaacaagccg cgtacgccca ccgcgtcggc aatcacaggg gcaacgacga ggctgaccga
    18121 aaacgtcaga ccgatactca aaccgatcat cgccatcgcg cgggtacgta cgccgtcgcg
    18181 cgtcaaatcc gccagcagcg cggtaaccgc cgcactgacc gctcccgcac cctgtatggc
    18241 gcgtgcggcg accagcatgg gcagcgtatc ggcggcggcg gcaaggaagc tgcccgctgc
    18301 aaacacaatc agcccgacat aaatggtttt cttgcgcccg aacttgtcgg aagcgatgcc
    18361 caaaggcagt tgcagcagag cctgtgtcag cccgtaaatg cccattgcca gcccgaccag
    18421 cgttttgttg ccttccgcgc cgggcagcga ggcggcatac accgccaata cgggcagcac
    18481 gaggaacata cccagcatac gcagcgcgta cacgccggaa agcgtcgtac tggcgcgcca
    18541 ttcgtgcgga aacatttgga tgcggttatc tcttgccatc atattttttc agacggcatc
    18601 aacagttgca atgccgtctg aacttccagt gaacagattt tcggattata caggattcgc
    18661 cgtatttcgg ttgcggcgcg ggttcaaaat caacgccact gccagcggtt gcgccacgcg
    18721 cccaaaacgg cgttcggata tttattgctg cccaagctgc cgttaaagcg ggcaagcgcg
    18781 cggacgatgt tgcctttttc aagattccgg taatggcgca ggatggtaca gccgtaacgc
    18841 aggttggtgc ggatgtcgaa caggttgtgc gccggtttgc cgatgtagtt tttccaaaac
    18901 ggcataacct gcatcaggcc gcgcgcgccg acaccgctga ttgcatactg gcggaacgcg
    18961 ctttccacct caatcagccc caacacaatc tgcgtatcca aaccggcccg gctgctttcg
    19021 tactggatat tgaccagcag cctgcgccgc tcctcctcct cggggacgaa ccttgccaaa
    19081 cgtgccgaca tggcagacaa ccaacgttcg ccctctttcg gattgtcaaa caccagcctc
    19141 ggcggattga cgctgccgac agaactcctc atcacggaag ccacatcgtc ggcaagcgtt
    19201 tcctcacgtt gcgcgccggc gtgcgccaga ggactgagca acaacgcacc ggcggcacac
    19261 aacaggcggc ggcgttgcag attaacgggt agggtatcgg tcggtttttt catagggaac
    19321 gggggcgcgt ccggacgttt cagacggcat taaatattca aacagacata attgctttca
    19381 atgcgaaaaa ccgcgcacaa aatccaagcg cggcatatcg ccctgccctt ttcgggcaaa
    19441 ccttgatttt accgccctca agaacgcttg tccaaacagg cacaggcaac accacccggg
    19501 cattttccgt tttcaccggt tatccgtcgt ccggattatg cagcagcacc atcagcgcat
    19561 cacgcttttc gggcggcagc aggcggagac ctttgcaaaa a
//
LOCUS       DAWFLT010000031        19159 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWFLT010000031 DAWFLT010000000
VERSION     DAWFLT010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19159
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.031"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..462
                     /locus_tag="WGR74_001118"
     CDS             <1..462
                     /locus_tag="WGR74_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0905545.1"
                     /translation="ESNSSTNTENSETQQNRIKIETGHQGNGSFHAASSLGLSAIYDF
                     KLNDKFKPYIGARVAYGYVKHQVHSVESKTKTVTSKPNGGPVKEGPTPKPAYHESNSI
                     SSLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGVRYHF"
     gene            complement(436..645)
                     /locus_tag="WGR74_001119"
     CDS             complement(436..645)
                     /locus_tag="WGR74_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905546.1"
                     /translation="MPSERLSDGIFPCVLNRIHETLDAFGGNVGCRAMFGGDQRTKYA
                     GKGLMPSEGSDGIGIGESEVVAHAQ"
     gene            complement(663..1220)
                     /locus_tag="WGR74_001120"
     CDS             complement(663..1220)
                     /locus_tag="WGR74_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0905547.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(1296..2930)
                     /locus_tag="WGR74_001121"
     CDS             complement(1296..2930)
                     /locus_tag="WGR74_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ0905548.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(3004..3333)
                     /locus_tag="WGR74_001122"
     CDS             complement(3004..3333)
                     /locus_tag="WGR74_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905549.1"
                     /translation="MSRCCLKRLQTASAVSAFSGGVLEVVRLCCRRLSVLFNPGVMLF
                     VPDCPVGGAAGFFQYEMLCPFFWQGRLQTGSNLAYDVFMSAGRLNGGRNPPHSRRFLA
                     LLCSVAL"
     gene            complement(3333..4439)
                     /gene="serC"
                     /locus_tag="WGR74_001123"
     CDS             complement(3333..4439)
                     /gene="serC"
                     /locus_tag="WGR74_001123"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ0905550.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSDGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4804..5235
                     /gene="rimP"
                     /locus_tag="WGR74_001124"
     CDS             4804..5235
                     /gene="rimP"
                     /locus_tag="WGR74_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ0905551.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTISFDGKTVQIELGNIDKARLRPEFKF"
     gene            5263..6765
                     /gene="nusA"
                     /locus_tag="WGR74_001125"
     CDS             5263..6765
                     /gene="nusA"
                     /locus_tag="WGR74_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ0905552.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTAIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6777..9665
                     /gene="infB"
                     /locus_tag="WGR74_001126"
     CDS             6777..9665
                     /gene="infB"
                     /locus_tag="WGR74_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ0905553.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPVEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGSKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPI
                     VVAVNKIDKDTANPERIRQELTQHEVIPDDWGGTVQFIDVSAKKGTNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMIKGYNEIMEGDQLECFDIVEVARTL"
     gene            complement(9876..11270)
                     /locus_tag="WGR74_001127"
     CDS             complement(9876..11270)
                     /locus_tag="WGR74_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HEZ0905554.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLIDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSGKYSEL
                     NLNQDKAASRRQYK"
     gene            complement(11344..12684)
                     /locus_tag="WGR74_001128"
     CDS             complement(11344..12684)
                     /locus_tag="WGR74_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ0905555.1"
                     /translation="MLNPSQKLVELVRILEEGGYIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRDTLERVRVGSFWLWVVAASMMFTAGFSGTYLLMDNQGLNFFLIL
                     AGVLGMNTLMLAVWLATLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWAVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWHEGT
                     WFTGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLAEKLEHWRNALTECGAAWLEPDRVAQEGRLKDQ"
     gene            12828..13454
                     /locus_tag="WGR74_001129"
     CDS             12828..13454
                     /locus_tag="WGR74_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ0905556.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSI
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIVIYVVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(13518..14936)
                     /locus_tag="WGR74_001130"
     CDS             complement(13518..14936)
                     /locus_tag="WGR74_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0905557.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSIFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWLSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIVALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFAFTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRIIYDDYL
                     MQKKARQDLSYQYEFYEFHDKG"
     gene            15263..15991
                     /locus_tag="WGR74_001131"
     CDS             15263..15991
                     /locus_tag="WGR74_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ0905558.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVANDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(16050..16538)
                     /locus_tag="WGR74_001132"
     CDS             complement(16050..16538)
                     /locus_tag="WGR74_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ0905559.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGIEPCVLCISQRLC
                     VLATALCAVIVLACKPKGRVGGLSGAVFISIPAVTGISVAAYQLWLQSLPPGTAPPCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGIALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(17227..17691)
                     /locus_tag="WGR74_001133"
     CDS             complement(17227..17691)
                     /locus_tag="WGR74_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0905560.1"
                     /translation="MKELDKIDFRILKILQKNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
     gene            17916..18974
                     /gene="alr"
                     /locus_tag="WGR74_001134"
     CDS             17916..18974
                     /gene="alr"
                     /locus_tag="WGR74_001134"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ0905561.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIKLRESGITHPIVLLEGVFEASEYEAVEQYSLWSAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGITEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
BASE COUNT         5039 a         5281 c         4834 g         4005 t
ORIGIN      
        1 gaaagtaatt cttctactaa tacagaaaat agcgagactc aacagaaccg cataaagatt
       61 gaaacaggac atcagggaaa cggcagcttc cacgccgctt cttctctcgg cttatccgcc
      121 atttacgatt tcaaactcaa cgataaattc aaaccctata tcggcgcgcg cgtcgcctac
      181 ggatacgtta agcatcaggt tcattcagtg gaaagcaaaa ccaagactgt tacctctaaa
      241 ccaaatggag gccctgtcaa agaaggtccg acccccaaac ctgcctatca cgaaagcaac
      301 agcatcagca gcttgggtct tggtgtcatc gccggtgtcg gtttcgacat cacgcccaag
      361 ctgaccttgg acaccggata ccgttaccac aactggggac gcttggaaaa cacccgcttc
      421 aaaacccacg aagtctcatt gggcgtgcgc taccacttct gattccccga taccgatgcc
      481 gtctgaacct tcagacggca tgagaccttt gcctgcgtac ttggtgcgct ggtcgcctcc
      541 gaacatggcg cgacacccga catttccgcc gaacgcatcg agcgtttcat gaatccggtt
      601 taaaacgcat ggaaaaatgc cgtctgaaag cctttcagac ggcatttcgc ttgagattcc
      661 gtttaccaat ggctgacaaa cgcttccaaa tcggtattct tgggcttatg cacttcctct
      721 gtcggcgtgc cgaccatcat cagcccgatg attttatcct tatccgcaca tccgaaagcc
      781 tcacgcaaca gggggctgtt gacccacatc cccgtaatcc agacattgtc gaaaccttgc
      841 gccgttgccg ccagttgcag cgcatacgcc gcacaacccg ccgtcagcat ctgctcccat
      901 tccggtttcg gcttaggcac atcgcggttc ggcgcaaacg ttacaccgat aaccatcggc
      961 gccatattgc ccactttttc cgcctttttc atcgcatcgt cgccgaaatt caattcggca
     1021 accgtttgct ttaacacatc gcgaaaacgt tgcaatccta cctcgccttg aatcacggta
     1081 aaacggaagg ggcgcatatt gccgtgatcg ggaacttggg ttgccgcctg aaatatttgt
     1141 tccaactccg ccgcatcggg ggcggggtgc ttcagctttt tggaagatcg gcggttcgtc
     1201 aataatttta aagcatccat atcgttatac tccggtcatc ggtcgggtgt tcggacacat
     1261 atgccgtccg aaggcttcag acggcatatc cggcatcagc gcggacggcg gcaggctgcc
     1321 aatatatcca tttccttccg ataggtttgg ctgttggaaa tgtccatcag ccccaatacc
     1381 gtgctgaaat agtggtcgtg cgaatattcg ttttccgccg ctttttgttt gaggcattgg
     1441 aaatctatgc ccccgtgttg gcggaaggct ttggaaaacc acataaccat cgggatatgc
     1501 gtctgcccgg aaggcgcgat ggcgtaaggc gcggcgtgca ggtacatccc gttttcgccc
     1561 aaactttcgc cgtggtcgga aacataatgc accacgcttt ccaaatcgtc gcggttttca
     1621 agtttgcgga taaccttgtc gataaactgg tccacataca aaaccgtatt gtcgtaagtg
     1681 ttgaccagcg tggcgcgggt gcatttgttg atttcgttgg tgtcgcaggt cggcgtgaat
     1741 ttgcgttcgg cttcggtata gcgttcgtaa tacgtcggcc cgtggctgcc gatggtatgc
     1801 aggattaaaa ccgcgtcttt atcgtttttg ttgaggactt cgtcgaactt ggtcagcagg
     1861 atattgtcga ggcactcgcc gttgcggcag tattcgggca ggttgagcga ggtaacgtcg
     1921 gtattcggca ctttgccgca cacgcccttg cagccggaat cgttttccaa ccaggtaact
     1981 tccacgccgg cgcgctgcac gatgtccagc aggttgtctt ggtgttcggc tttgatttcg
     2041 tcataatccg tgcggtcgaa ggttgagaac atacacggca gggagtgcgc ggtcgatgta
     2101 ccgcagcttc tgacctgagg gaaattgaca atttcatcgc cgcgcgcggc aagcagcggc
     2161 gtagtttggc ggctgtaacc gttcaaaccc cagttggcgg cacgcgtggt ctcgcccacg
     2221 accagcacca cgaaacggcg caggctgccg gccgggcggt tttgcacgac cgccatatcc
     2281 aattgcgtat aaggaatatt ggaacgcttc caatctttgt atttcgacac gcccgcgccg
     2341 atgaaattag atggcacaat cagatgggtt actgatttat tgttgcggaa aaacgaggcg
     2401 taatcctgat attgcaacat tgcgatgccc aacgcgcaca aaaaggaaac ggcggcaagc
     2461 acaaggcgcg tcaaaagctc cttataccaa acgcggtatt taaccttgac ggcgatatac
     2521 gccagcgcgg gcaatacgcc caaacataca atccacagca catagcccgg cgtaatcagg
     2581 cgcgcgcttt cggcagccgt agtttgcaag acattattca acatcgactt gttgaaatag
     2641 atattgaaaa atatttcttg gtaagacacc gccgcactga taaccaatat caacggaatc
     2701 aataccttat gcacgaaagg cagggcaatg acgtgaaaaa cgaaattact taaaaaaaac
     2761 agcaccaccg gcatcgtata gaggaagata tccgccccgg tgccgttaaa aggatgaagc
     2821 tcgacaactt tggcaaaaaa ggcgtaattc aataccagcg aggaatacag ggaaaggaag
     2881 gcaatcagcg cggaagagcc gagcttcggc ctcaggttcg gttttatcat ttggaatgtg
     2941 tcggataagg gttggaaaag gcatccggca tttggaatcc ggattattga aaaagattct
     3001 taattataag gcaacggagc aaagcagggc aagaaaacgg cggctgtgcg gggggttccg
     3061 cccgccattc aaacgtccgg cagacataaa aacatcgtaa gcaagattcg aaccggtctg
     3121 caaccgcccc tgccagaaaa acgggcaaag catttcatat tggaaaaacc cggccgcgcc
     3181 gccgacggga cagtccggca caaacagcat cacgcccgga ttgaaaagga cggatagccg
     3241 ccggcagcac aatcttacca cctccaaaac gccgccggag aacgccgaaa cagccgatgc
     3301 cgtctgaagc cgcttcagac aacatcggga catcaaccgt aacgccgttg gaaatcgcgc
     3361 ataaaatctg ccaaagcccg cacgccttca agcggcatcg cattataaat gctggcacgc
     3421 ataccgccga cggttttata gcccttaagc aggcacaagc cctgcaattc ggcttccagc
     3481 acaaaacggc ggtcaagctc ctcatccccc gtttggaaca cgacattcat tttagaacgc
     3541 gcattcggac ggatacggtt gatatagaaa ccgccgctgc catctatcgt ctcatacaag
     3601 gtttgcgcct tcagccgatt gaccgcttca atttttttca caccgccctg cgcctgcagc
     3661 cagcggaaca ccagccccga catataaatc gcgtaagttg acggcgtgtt gtacataccg
     3721 tcgcggttca aatgcgaacg gtagttgaac acatcgggaa tatcgttcgg acaacgctcg
     3781 agcaaatcct cacgcacaat caccaccgta acccctgccg gcccgatgtt tttttgcgcg
     3841 cctgcgtaaa tcagtccgta gtcggcaaca tcaaactcac gcgacagaat ctcgctggac
     3901 atatcgcaca ccaacggcgg catgccgtct gaaaggcgag gcacttcacg gtattgcagc
     3961 ccgttgaccg tttcattgac ggcaaaatgg acaaacgccg aatcgggtgc aacatcccac
     4021 gtttccacag gcggcaggtc gagatagtcg aactgctcgc cgccatgcgc cgccaaacgg
     4081 atttccgtat cggtcaaacg gctcatctgt tcataagcga tacggctcca gttgcccgtt
     4141 accaccgcgt cggcagtgcg gaaaccgtgt gccagattca tggctgccat attaaattgg
     4201 gttgttgctc caccctgcag aaacaatatc ttatagttgt caggcacttt caaaagctgc
     4261 ctcaaatcct gttccgcatg atgcaggatg ctcaaaaaca tttccgaacg gtggctcatc
     4321 gccatcacag gaaaacccgt accgttgtag tccaacattt cctgccgcgc cgtttccaac
     4381 acggcttcgg gcaatacggc agggccggcg gaaaaattgt aaatcggata aagagacata
     4441 atgcagcctt gattctgaac aataacccgc ccgattttag gcttgcagca ggctttgtgc
     4501 aaggatggga aatacctgtc ctccgcccga ttccatgccg cccgaacacg ggaaacaaca
     4561 tcaatatatt gatttacaaa tataaaaatc atgctagcga caaatagata catttgtttt
     4621 gtcaacaata ttcacgattt cccattacaa acctccctta cacccgcttt tttccgtccc
     4681 aaaaacacaa aataaatcaa cactttcatt tctccgcaaa agcggttata atcacgccga
     4741 tttttcaact ttgacgaaaa atggccacgg ggccattttt ttattatgta catagggagc
     4801 agcatggata tccaaaccat cctcgaaaaa accctgcccg gcctgggcta cgaactggtc
     4861 gatttcgaac tgaccgcgca aggaacattg cgcgtgttca tcgataaaga aggcggcatt
     4921 accgtcgaag actgcgcaac cgtcagcaac cacttgagcc gcgtcttcat ggtggaagac
     4981 atcgactaca aaaacctgga aatttccagc cccggactcg atcgcccctt gaaaaaagcc
     5041 gccgacttcg tgcgctttgc cggtcagaat gccaaaatca aaacccgcct gccgatagat
     5101 ggtcagaaaa actttatcgg taaaatcgaa ggctgcgaaa acgataccgt taccatatcc
     5161 ttcgacggca aaaccgtaca aatcgaattg ggcaacatcg acaaagcccg cctgcgcccc
     5221 gaattcaaat tctaaaacac aacaatattg gagatgttca aaatgagtcg tgaaatgtta
     5281 cagctggcag aagcactggc aagcgaaaaa aacgttgatg cggaagtcgt cttccaagca
     5341 ctggaattcg ccctgtctac cgccgccaag aaaaaggcag accgcgaaca catggatgtc
     5401 cgcgtacaaa tcaaccgcga caccggcgaa taccaaacct tccgccgttg gctgattgtc
     5461 gccgatgaag actataccta tcccgatgtc gaaaaaacca tcgaggaaat ccaagaggaa
     5521 attcccggca ccgccatcca aatcggcgaa tactatgaag agcagctgcc caacgaaggc
     5581 ttcggccgcc aagccgcgca aaccgccaaa caaatcatcc tgcaacgcat ccgcgatgcc
     5641 gagcgcgagc agaatctgaa cgagtttctc gccgtcaaag aagacatcgt gtccggcacg
     5701 gtcaaacgcg tcgaacgcca cggcatcatc gtcgaagtcg ttgccggcaa actggacgcg
     5761 ctgattccgc gcgaccaaat gattccgcgc gaaaacttcc gcagcggcga ccgcatccgc
     5821 gccctcttcc tgcgcgtcga agaaatcggc aacaccggcc gcaaacaagt catcctgagc
     5881 cgtacttccg gcgatttcct cgtcaaactg tacgccaatg aagtacctga aattgcagac
     5941 ggcatgcttg aaatccgcgc tgtcgcccgc gacccgggac aacgtgccaa agtcgccgtc
     6001 aaagccaacg accagcgcat cgatccgcaa ggcacctgta tcggcgttcg cggttcgcgt
     6061 gtcaatgccg tcagcaacga attgtccggc gagcgcatcg acgttgtcct ttggtcgccc
     6121 gagcctgccc aattcgtcat gagcgcgctc tcacccgccg aagtcagccg catcgtcatc
     6181 gacgaagaca aacacgccgt cgatgtcatc gttgccgaag accagctcgc gctcgccatc
     6241 ggacgcggcg gtcaaaacgt gcgccttgct tccgacctga ccggctggca gctcaacatc
     6301 atgacttccg ccgaggcaga cgaacgcaat gcggcagaag atgccgccat ccgccgcctg
     6361 tttatggatc acttgaacgt ggacgaagaa accgccgacg tactggttca ggaaggtttt
     6421 gcaaccttgg aagaagtcgc ctatgttcct gccgccgaac tgcttgccat tgaaggattt
     6481 gacgaagaaa tcgtcgatat gctccgcaac cgcgcccgcg atgccatcct gaccatggcg
     6541 attgccgccg aagaaaaact gggcgaagta tccgacgata tgcgcaacct cgaaggcata
     6601 gatgccgata tgctccgcag ccttgccgaa gcaggcatta ccacccgcga cgacttggca
     6661 gagcttgctg tggacgaact gattgaaatc accggtgtaa acgaagaaac cgcaaaagcc
     6721 gtcatcctga ccgcacgcga acactggttt accgaagaca aataaagggg gtacagatga
     6781 gtaacacaac cgtagaacaa tttgccgccg agctgaaacg ccccgtcgaa gacctgttga
     6841 aacagttgaa agaagccggc gtcagcaaaa acagcggcag cgattccctg acgctggacg
     6901 acaaacagct tctgaacgcc tacctgacca agaaaaacgg cagcaacggc ggcaccatca
     6961 gcatccgccg caccaaaacc gaagtcagca ccgttgacgg cgtaaaagtc gaaacacgca
     7021 aacgcggacg cactgtcaac attccttctg ccgaagaatt ggcagcacag gtaaaagccg
     7081 cccaaaccca agccgcacct gtccggccgg agcagacggc agaagacgcg gcaaaagccc
     7141 gagccgaagc tgccgcacgt gcagaagccc gtgccaaggc agaagcggaa gcggcaaaac
     7201 tgaaagcggc aaaagcaggc aacaaagcca aacctgccgc gcagaaaccc accgaagcaa
     7261 aagccgaaac cgcacccgtt gcggcggaaa ccaaacccgc cgaagaaagc aaagcggaaa
     7321 aagcccaagc cgacaaaatg ccgtctaaaa aacccgtcga gcccaaagaa aaagccgcca
     7381 agccgaaaca cgagcgaaac ggcaaaggca aagatgccaa aaaaccggcg aaacctgccg
     7441 cacctgccgt gccgcaaccc gtggtcagcg cggaagaaca ggcgcaacgc gatgaagaag
     7501 cacgccgtgc cgccgcactt cgcgcccacc aggaagccct gttgaaagag aaacaggaac
     7561 gccaggcacg ccgcgaagcc atgaaacaac aggcagaaca acaggcaaaa gccgcacagg
     7621 aagccaaaac cggcagacag cgtcccgcca aacctgccga gaaaccgcag gcagccgcgc
     7681 cggccgtcga aaacaaacct gtcaatccgg caaaagcgaa aaaagaagac cgccgcaacc
     7741 gcgatgacga aggtcaaggc cgcaacgcca aaggcaaagg cggaaaaggc gggcgcgacc
     7801 gcaacaatgc acgcaacggc gacgacgagc gcgtacgcgg cagcaaaaaa ggcaaaaaac
     7861 tcaaactcga gccgaatcaa cacgccttcc aagcaccgac cgaacccgtc gttcatgaag
     7921 ttttggttcc cgaaaccatt accgttgccg atttggcgca caaaatggcg gtcaaaggcg
     7981 tggaagtggt caaagccctg atgaagatgg gcatgatggt aaccatcaac caatccatcg
     8041 accaagacac cgccctgatt gtcgtcgaag aactcggaca catcggcaaa cccgccgccg
     8101 ccgacgatcc cgaagccttc ttggacgagg gcgcggaagc ggtggaagcc gaagcattgc
     8161 cgcgtccgcc cgtcgttacc gtcatgggac acgtcgacca cggcaaaacc tcgctgctgg
     8221 actacatccg ccgtgccaaa gtggtacagg gcgaagcggg cggcattacc caacacatcg
     8281 gtgcatacca cgttgaaacc ccacgcggcg tgattacctt cttggatact ccgggtcacg
     8341 aagcgtttac cgccatgcgc gcgcgcggcg cgaaagcgac cgacatcgtg attctcgtgg
     8401 ttgccgcaga cgacggtgta atgccgcaaa ccatcgaagc cattgcacac gccaaagcgg
     8461 caggcgttcc gattgtggtt gccgtcaaca aaatcgataa agacactgcc aaccccgaac
     8521 gcatccgcca ggaactgacc caacacgaag tgatccccga tgattggggc ggcacggttc
     8581 aattcatcga cgtttctgcg aaaaaaggaa caaacatcga cgcgctgctc gaagcagtct
     8641 tgctcgaagc cgaagtattg gaactgaccg cacctgtcga tgcgcccgcc aaaggcatca
     8701 tcgtcgaggc gcgcttggac aaaggccgcg gcgcggttgc cacattgctg gttcaaagcg
     8761 gtacgctgaa aaaaggcgac atgctgctgg ccggtacggc attcggcaaa atccgcgcga
     8821 tggtcgatga aaacggcaaa tccattaccg aagccggccc gtccatcccc gtcgaaatcc
     8881 tcggcttgtc cgacgtaccg aatgcgggtg aagacgcgat ggtattggcg gacgagaaaa
     8941 aagcgcgcga aatcgccctc ttccgccaag gcaaataccg cgacgtgcgc cttgccaaac
     9001 agcaggcggc gaagctggaa aatatgttca acaatatggg cgaaacccag gcccaatctt
     9061 tgtcggtcat catcaaggca gacgtgcagg gctcttacga ggctttggcg ggcagcctga
     9121 aaaaactgtc cacagacgaa gtgaaagtga acgtgttgca cagcggcgtg ggcggcatta
     9181 ccgaatcgga tgtcaacctt gccatcgctt cgggcgcatt cattatcggc tttaacgtgc
     9241 gtgcagatgc ctcttcgcgc aaacttgccg aaaatgaaaa cgtggaaatc cgctactaca
     9301 acatcatcta tgatgccatc gacgacgtga aagcggcgat gagcggtatg ctttctcccg
     9361 aagagaaaga acaggttacc ggtacggtcg aaatccgtca ggttatctcc gtttccaaag
     9421 tcggcaatat tgcaggctgt atggttaccg acggcgtggt caaacgcgat tcccatgtcc
     9481 gcctcatccg caacaacgtg gttatccaca cgggcgaact ggcttcgttg aaacgctata
     9541 aagacgatgt aaaagaagtc cgcatgggct tcgagtgcgg cctgatgatt aaaggctaca
     9601 acgaaattat ggaaggcgac caactcgaat gtttcgacat cgtcgaagtg gcacgcaccc
     9661 tgtgattttg atgcggaaaa atgccgtctg aagcgttcag acggcatacg aaacgggttc
     9721 tgtatcatac agaacccgtt ttttgtcgca aatcggcttc agacagccct cttgccttat
     9781 cccgatttga atctgacttg ccatacaaac aggcttcaga cggcattata gtgaattaaa
     9841 tttaaaccgg tacagcgttg gctcgccttg ccgtactatt tgtactgtct gcggctcgcc
     9901 gccttgtcct gatttaaatt taattcacta tattttcccg acaggcggat ttgaagtttc
     9961 tccacatatc cggcgcgttc ggcgcggctg gtttcggggc ggtgcgtatt gagcgacgac
    10021 catttccaat gactgcgggc tttgttgagt tcgggcggga gtctggcggc gtcccacggg
    10081 gctttgcggc tgtgcagctc gatatccgac tgtgccgcgt gtccgcgcgt ttgcaggacg
    10141 tggagcaaat cgagggcgcg ggcggcgagc agggtcaagg tttcagggtc ggtgtgcagg
    10201 gtttggcggc cagcgagttt gtcggaaatg gtgcgggtat tgggcaggat gccgcccaaa
    10261 aagccgccga ttgccgtacc caatccgagc gagccgccga gtgtggcgat gtccagcccc
    10321 aagccgatga gcgcgccagt tgccgcgccc gtgccggtgc ggatgccgta ttgtttgagc
    10381 aattcgctgt cgaacgggtc ttggcggaag gcttgcggca tccagtcgcc gccgtcgatt
    10441 tcgctgtggt agaaacggta aagggcaaac agccgctgct gcatctgccg ttcgagttgg
    10501 cgtatttccg cctgcatggt ttgcagcacg gtggcggtat cctcgttttc gtccacttcc
    10561 tgcctgaagg cggcggcatc aattaaaaag tcggcgattt cgcggcgcgc ttcgccgtcc
    10621 agccgctgcc attcgcgccg gcgcatggct gtcaggcggt caagtgtgct gcgttcgggc
    10681 aacatggtgg cgaggttttc ccacaggcgt agttcgcctt caaaatcaaa ggcgacggtg
    10741 tcgaaccctg cgaaaacgtg caggtttctc ctcgccagca tggttgtcca cgattcggga
    10801 ggctgtccgc cggtaaagtt gaacacgggc ataaccggtt tggcacacca tgaaaggatg
    10861 gtcaactcgt ccctgtattt gtcgaggacg ggttcgcgcg cgtcgatgac gtacattgcc
    10921 atatcgcttt gcaagacttg ccgtaagact ttggcttcct gattgaaatc atggtgcgcg
    10981 ccgtggctgc cgagaaactg ttgcagccgt tcgatgccgt ctgaacgatt gtccgtatgg
    11041 ttttccagcc attccagcac gccgcccgcg tcttcgagtc cgggcgtgtc gtacaggaaa
    11101 accagcgtgt ctgcgccgtc gctgatggcg gcttcttcga catgacgcgt ggtcgatggg
    11161 gcgtttctga cttcgccgaa accgctgtcg cgcaaaaggg tacgaaggag cgaggttttg
    11221 ccggtattgg tatgcccgac gacggcgagg gaaaggggtt gtttgttcat gatgtttttg
    11281 aagaatggat tttcagacgg tcttttttca gaatggcggc ttaacagaac atttcaagtg
    11341 agtttattgg tctttcaaac ggccttcctg cgccaccctg tcaggctcaa gccacgccgc
    11401 gccgcattcg gtcagcgcgt tacgccaatg ttccagcttt tccgcaaggt cgtctgaaag
    11461 cccctgttcc gccagaagct gcaccaccgc gccgccctgc gccgcttccg agagccggac
    11521 aatctgccgc aacacgcccc ggtccggcac agtttgggcg cgcacgccga taagcagttg
    11581 cgccggtttc tgcttcagct ctgtctccag cgcggcaacc tgttcccgat tggcggcaac
    11641 gcccttatcc agccattcct gcgccagcct gcccgtaaac catgtccctt cgtgccactc
    11701 ggtctccagc atgaccgccc atttcggcgc atcgttcaag acgattttcg gcgaaacggc
    11761 ggacacggtt tcccgacgcg tatccgcatc ggtaattttg ttctgccagc ggcggatgac
    11821 cgcctgataa tagggctttt ccaaatccaa tccgttttcg cttgttttca aaaggatttt
    11881 gcataccgcc caagccaaga ggcgcggcag gatgccgtag caggcgatac tgccgaccag
    11941 cagccccgac caagcccgcg catcggcaat attgccgttc agacggcctt cgatgaccgc
    12001 ccgcgcatca ggcacgggaa aacccagttt cgcaggcagc catgccaaca tttccaccgc
    12061 gcgtaccgaa gcggcattgc tcaacagcgt gctttcccag ttgaacgtat attgccgcac
    12121 caaaagcagc agcaataccg acaccagcat tccgagcagc gtgcagagcc acaggctgtg
    12181 cgacgttgcg cctattttcc aacgtaccga aggttgccgc cactcgtccg catacagccg
    12241 caacaccgcc tgattgacag ggtctttgcc ccgaaaccac gtcgccggac tgctgaaaaa
    12301 acgccccact ttcacgcgca ggaacaacgt tgccaaccat actgctaaca tcagcgtatt
    12361 catgcccaac acgcccgcca aaatcaggaa gaaattcagc ccctgattgt ccatcagaag
    12421 ataagtgcct gaaaatccgg cggtaaacat catcgatgct gccaccaccc acaaccagaa
    12481 cgaccccaca cgcacacgtt ccaacgtgtc ccgcagcata cggttcctgt cgatcatctc
    12541 cgcccgacgg atgatttttt cctccgtact gccgtccacg cggcgcaaag cctccgtcgc
    12601 ctgcacggga tcgccgctga aaatatagcc gccctcttcc aaaatgcgaa ccaattcaac
    12661 cagtttttgg gaaggattca acataaaatg ccatctgaaa ataaaaaaac agattttaac
    12721 acacgcattt tcaagaatat tcacagtgta ggcaaagagt aaatcccaca cagaggcaaa
    12781 agtatcggcg taaactgact gcctctactt tcccgaaaga ttgtgcgatg tatacaggcg
    12841 aacgcttcaa tacttacagc catttgagcg gtttgattct ggcggcggca ggtttggcgc
    12901 tgatgctgct gaaaaccata ggacacgggg acggctaccg tatcttcagc gtatcgattt
    12961 acggcatcag ccttcttctg ctctatttga gttcctcgct gtaccacgga attgcagccg
    13021 gaaaactgaa aagcattttg aaaaaaaccg accactgcat gatttatgtg ctgattgccg
    13081 gaagctacac accgtttgca ctggtttctt tgagaaacgg gccgggctgg acggtatttt
    13141 cactgtcctg gctgctggcg gctgcaggaa tcgcacaaga actcaccatc ggacggaaaa
    13201 gcgaaaaacg tctgctgtct attgtgattt atgtcgtcat gggttggatg gtcttggcgg
    13261 taatgaaatc cctgacagcc tcactcccac cggcaggact ggcttggctg gcggcaggcg
    13321 gtatgctgta cagtgtcggc atttactggt ttgtaaacga tgaaaaaatc cgacacgggc
    13381 acggaatctg gcatctgttc gtattgggcg gcagcatcac ccaatttgtc agcgtgtacg
    13441 gttatgtaat ctgaatgccg tctgaaaagc aaaacctccc gttcctgaag attgggaggt
    13501 tttctctttg ccggacatca gcccttgtcg tggaactcgt agaattcata ctgataggac
    13561 aaatcctgtc tcgccttttt ctgcatcagg tagtcatcat aaatgatgcg gatttcctta
    13621 cgcagcaaga ataaggcaat caggttgggg ataaccataa agccgttgaa catatcggac
    13681 aggctccaaa ccaaatcgac tttgccgagc gtacccaaaa caatggcaag cagaaccaat
    13741 gcgcgataga tgcccaagtg tcttcccctg aaaagaaaac ggatgttgga ctcgccgaaa
    13801 taataccaac cgataatggt cgtaaaggcg aagaaggtca gacacacggc aagcaattgc
    13861 gaaccgaagc ccggaaatgc cttgttaaag gcaaattgag taaccgccgc gccctgttcg
    13921 cccgaaaggt tggcatcggt cagcaggata atcaatgcag ttgccgtaca taccaaaatc
    13981 gtatcgataa acacaccgac aaatgccgcc ataccttgct gcacaggatg cttcacatcc
    14041 gcagtcgcgt gggcgtgcgg agtcgaaccc atacctgctt cgttggaaaa cagaccgcgc
    14101 gccacgccga aacgtatcgc ttcgcgcata ccgatacccg cagcaccgcc caaaacggct
    14161 tcgggattga aggcggcggt aaagatgtgg ttgaacatcg gcacaatatg gtcggaaaat
    14221 tcaaacagga taacgacggc gcacaaaata taaacaaccg ccataaacgg cacgacaaac
    14281 tgggcaatat tggcaatacg gttcacgccg ccaatcacaa ccatgcccgc aaggacggca
    14341 agcacaatac cgactgccaa agaaggcaca tcaaatgcaa tggtaacggc agaagcgatg
    14401 gagtttgcct gagtcgcatt gccgataaag cccaacgcga caatcagcgc gatggagaaa
    14461 aagcccgaca ggaaacgcgc cgcgccgcga ccgattttcg gagtcagacc gtgcgtaata
    14521 taaaacgccg gcccgccgat gtatttgccg tggctgacga cgcggtattt ctgcgccagc
    14581 agtgcctccg caaaaatcgt ggacatcccc aaaacggcag aaagccacat ccaaaaaatc
    14641 gcgcccggcc cgcctgcggt gatggcggtc gccacgccgg cgacgttgcc cgtaccgatt
    14701 tgcgcggata tggcaactgc caacgcctga aactgcgata aagacttgtc gtctttatcg
    14761 cctttggcaa acaagccgcc gaatatggat ttgaatcccg cgcccagctt ggtaatctgc
    14821 ggcgcaccga gatacagcgt aaaaaacagg ccgataccca aaagcgcgta aatcagcagg
    14881 tagtcccaaa ggaaccgatt gactgtaccc accagaacag acaatatatt ttccataaaa
    14941 taaaccttat cttacaatta aaatgactgc cttccaaaag acattccaat aaggaaacac
    15001 ggcgagcaga ccgtatttgc cgcaacagat gccttaaatt gtcaacaatc ggggagaagt
    15061 tgtgccatca taccgtaaaa tatcgttaat taaaatattt ctatattttt aagcggaaag
    15121 cggaggaaat cgctttcaga cggcatagac aacggcacgg cataaaacag gatattttgg
    15181 gtgcttgcaa cttatgttaa aatgccgacc gtaaaaaatc tgacaaaaac agattaatta
    15241 tttgaaataa gaaaggaaat ttatggcagg ccatagcaag tgggcgaata tccagcataa
    15301 aaaagcccgt caggatgcca aacgcggcaa aatcttcacc cgtttaatca aagaaatcac
    15361 cgttgcggcg cgtatgggcg gcggcgatcc cggtgccaac ccgcgcctgc gcctggcttt
    15421 ggaaaaagca gccgaaaaca atatgcccaa agacaatgtg caacgcgcca tcgacaaagg
    15481 cacgggcaac ttggaaggcg tggaatacat cgagttgcgc tacgaaggct acggcatcgg
    15541 cggcgcggct ttgatggtgg actgcctgac cgacaacaaa acccgcaccg ttgcggacgt
    15601 acgccacgcg tttaccaaaa acggcggcaa cttgggtacc gacggctgcg tggcgttcaa
    15661 cttcgtgcat cagggctatt tggtatttga acccggcgtt gacgaagacg cgctgatgga
    15721 agcggctttg gaagccggtg cggaagacgt ggttgccaac gacgacggtt ccatcgaagt
    15781 cattaccgcg ccaaacgatt gggcgggcgt aaaatccgct ttggaggcgg caggttacaa
    15841 atccgttgac ggcgacgtta cgatgcgcgc ccaaaacgaa accgaactct ctggcgacga
    15901 tgccgtcaaa atgcaaaaac tgattgacgc gctggaagac ttggacgacg tgcaagacgt
    15961 ttacacttcc gccgtattga atttggactg atacgcagca cagcagacat acaataaaat
    16021 gccgtctgaa cctttcagac ggcattgatt tatttagccc ttgcccacgc ccaccaaacc
    16081 gtcaggacaa ccgccagaaa atacgccgcg ctccaaacag gcaaagcaat tcccagcaga
    16141 taatccggtt cggcacaatt tccgaacccg cgcacgacag gctcgaacca atcaaacaaa
    16201 ggccagcctt tcaagcggaa cgtccacggc gcgccgcatg gaggagccgt acccggcggc
    16261 agcgactgca gccacaactg atatgccgca acagaaatac ccgtaacggc cggaatgctg
    16321 ataaacactg cgcctgacag tccgcccacc ctgcccttgg gcttacacgc cagcacaatt
    16381 accgcacata atgcggttgc caagacgcac aaccgctgac tgatacacaa aacgcaaggc
    16441 tcaataccca aaacatactg tgccgccaaa gagccggcaa atgcacagac cgaaacggca
    16501 aacagcagcc aaacggcttt tctaaataac ggggtcattt tctcaacaca ccaatcaaaa
    16561 taccgatatg ccgattttgc tggatatatc ccgagatggc aagggacaaa acggcgattt
    16621 gcccgcacaa tgcccacaaa aaatacatcc ggaggatttg aatttcagca aatttagcga
    16681 atcaaaagtt tatttcaatg aaatcatatg atttttttga ataagcggat tgatgggttt
    16741 ttgaaggaat ttgttaccgg atagccatcg ggcaagtttt ttgcaaaatt tgaatcggtc
    16801 gggtaaattt tcaaaaaata attgacagcg gataagaaac ggcggataat tcccgccgtc
    16861 gagttgcttg atgcagcttg atttttctcc tctatttctc ctttgtagac ttggcacaca
    16921 ttcaaccgga tgtgtgcatt tttttatctc cgcttttttt gaaatttaat tacttttatt
    16981 gtttgaaatt ccattcttta agaaatttaa caagaaaaag cttgcttttt atgactgata
    17041 gcggcataat ttgacccgtt agctggttcg agtagtcagt taatagtttc tcctctattt
    17101 ctcctttgta gacttggcac acattctatc gaatgtgtgc atttttttat ctaaaacgac
    17161 aacatgccat acgttttgtt gttatatttc attcaggtgt caaactgcat agccagcctg
    17221 aaatattcaa tccaaatcca aaaccggatt ttctttgacc tcctccatca caacataact
    17281 cctactctcc gaagcggcag gcagttgcaa taggatattg cctagcatat cccgataggc
    17341 agacatatcg ggcaaacgta ctttaatcag ataatcgtat tcgcccgaca ccaagtggca
    17401 ttccataatt tgcggaattt tcagcacttc ttttttgaaa tcttcgaaaa tattgcccga
    17461 tttggattgc agcttcagct cgacaaaaac caataaaggt ttgcccaaca gatggggatt
    17521 gagatgggcg tgatagccgg aaatataatg ctcccgctcc aaacggcgta cccgttcggt
    17581 aactggagta gtggacaatc ctactttctc ggcaagctcc gtcatcggaa tacgggcatt
    17641 tttctgcagg atcttaagga tacggaaatc aatcttatct aactctttca tttgtcttta
    17701 ccttattgtt tattattgat tttaatggtt gggatgctga taaggaaaga caattatatc
    17761 aggaaaacgc aagcctcctt tccctatggc aaacccaacc ccacctccga agagtaaggt
    17821 tctttaatat tttaaaagcc ttgccgttgg attataatcc cccaaccgat ttcttaattt
    17881 tgctaataaa cacttgtttg gtaaggaatg aatttatgcg tcctttgaac gtgcagatca
    17941 ggttgggcaa ccttaggcac aattatcgga ttttgaagga aatgcacgga ggcaagctgt
    18001 tggcggtagt gaaggccgac gcatacggac acggtgcggt cagatgtgct ttcgcgctgg
    18061 cagacttggc agatggtttc gccgtggcca caatcgatga gggaatcaag ctgcgggaga
    18121 gcggcattac ccatccgatt gtccttttgg aaggcgtatt tgaagcatca gagtacgaag
    18181 cggtcgaaca atactcgctc tggtcggcag tcggaaacca atggcaactt gaggctttgc
    18241 tttcccgtca ttggaaaaaa cctgtcaagg tctggttgaa aatggattcg ggaatgcacc
    18301 gtaccggttt tttccctcat gattacgctt cggcatatgc ggcattgaag cagtcggaat
    18361 atgtggacag tattgtcaaa ttctcgcatt tctcctgtgc ggacgaaccc gaaagcggta
    18421 taacggaaat acagatggaa gcattcgatt tgggtactga agggctggaa ggcgaagaaa
    18481 gccttgccaa ctccgccgct attttgaatg ttcccgaagc acgcagggac tgggggcgag
    18541 ccggcttggc gttgtacggc atttccccgt tcggaggcag cgatgacagg ctgaaacccg
    18601 tgatgaggct ttccacccgt attttcggcg aacgcgtttt acagccgcac tcccctatcg
    18661 gttatggcgc aacattttat accagtaaat ctacgcgcgt cggcctgatt gcctgcggtt
    18721 atgcggacgg ttatccgcgc cgcgccccaa gcaattcccc cgtcgccgtc gacggtaaat
    18781 tgacccgggt catcggcagg gtctctatgg atatgatgac catcgagctg gacgcttcgc
    18841 aagaaggttt gggacacgag gtcgaactgt ggggcgatac ggttaatatc aataccgttg
    18901 ccgaagcggc cggaaccatc ccttacgaat tgatgtgcaa tatcaaacgt gcaaaattca
    18961 cttatatcga gtaatcaagt ccaaacaaaa atgccgtctg aagcctttca gacggcataa
    19021 atgcgttttg acaaaatcag tatagtggat taacaaaaat caggacaagg cgacgaagcc
    19081 gcagacagta cagatagtac ggcaaggcga ggcaacgctg tactggttta aatttagggg
    19141 ctgtcctgga taactagga
//
LOCUS       DAWFLT010000032        18798 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWFLT010000032 DAWFLT010000000
VERSION     DAWFLT010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18798
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.032"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="WGR74_001135"
     CDS             complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="WGR74_001135"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0905562.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGAAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTSGLAGMAKG
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAAPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELAAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1906..2343)
                     /locus_tag="WGR74_001136"
     CDS             complement(1906..2343)
                     /locus_tag="WGR74_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905563.1"
                     /translation="MGNFLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKA
                     THGPSVKNAVYAHQIETGLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQ
                     YSIFEYEVEHPENPNEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(2437..4029)
                     /gene="aceF"
                     /locus_tag="WGR74_001137"
     CDS             complement(2437..4029)
                     /gene="aceF"
                     /locus_tag="WGR74_001137"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ0905564.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKVGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPVA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGSAIIEVETAGSAAAAPAQAAAPAPAAAPAPTAVAAPAPSAPAAA
                     KIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQGGAAK
                     PAAAGASLGGGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHE
                     EADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKN
                     YFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTI
                     SSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVIDG
                     AAGMRFTVFLAKLLKDFRRITL"
     gene            complement(4175..6838)
                     /gene="aceE"
                     /locus_tag="WGR74_001138"
     CDS             complement(4175..6838)
                     /gene="aceE"
                     /locus_tag="WGR74_001138"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ0905565.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGGGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDTQLKSSGDREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEAERFNRLNPLETAKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRSFFEVDRYNVAVAALSA
                     LADQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7532..9244
                     /locus_tag="WGR74_001139"
     CDS             7532..9244
                     /locus_tag="WGR74_001139"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ0905566.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASSAAIVEQFGANGG
                     SLGSVGFAGKVYADFVTEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDAVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     SLNA"
     gene            9307..9963
                     /locus_tag="WGR74_001140"
     CDS             9307..9963
                     /locus_tag="WGR74_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ0905567.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRRKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPEKVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9973..10428)
                     /gene="ruvX"
                     /locus_tag="WGR74_001141"
     CDS             complement(9973..10428)
                     /gene="ruvX"
                     /locus_tag="WGR74_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ0905568.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(10421..10969)
                     /locus_tag="WGR74_001142"
     CDS             complement(10421..10969)
                     /locus_tag="WGR74_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ0905569.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10971..11531)
                     /locus_tag="WGR74_001143"
     CDS             complement(10971..11531)
                     /locus_tag="WGR74_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ0905570.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11859..13664
                     /locus_tag="WGR74_001144"
     CDS             11859..13664
                     /locus_tag="WGR74_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ0905571.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13784..15268
                     /locus_tag="WGR74_001145"
     CDS             13784..15268
                     /locus_tag="WGR74_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ0905572.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSSIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLNGAVGVSAAFLPPDALVVSTKGREG
                     TEGFALKARPEDYIFKAGTDPLAGIPAELKTIPMTVLINSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
     gene            15597..17624
                     /gene="uvrB"
                     /locus_tag="WGR74_001146"
     CDS             15597..17624
                     /gene="uvrB"
                     /locus_tag="WGR74_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ0905573.1"
                     /translation="MEVIRYPDSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLHQRVGRYTVFPSSHYVTPRDTVLRACDSIKEELRER
                     IDFFTKENRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPSNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRNIKENLLFGAE"
     gene            17692..18456
                     /locus_tag="WGR74_001147"
                     /pseudo
     CDS             17692..18456
                     /locus_tag="WGR74_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245990.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
     gene            complement(18621..>18798)
                     /locus_tag="WGR74_001148"
     CDS             complement(18621..>18798)
                     /locus_tag="WGR74_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0905574.1"
                     /translation="DKLDVSGFIHYRINHSKEFADRQNHINGIENFWNQAKRVLRKYN
                     GIDCKSFPLFLKEC"
BASE COUNT         4709 a         4674 c         4983 g         4432 t
ORIGIN      
        1 ccggaggaaa tattttaatc cgctgtaaaa tagaaaatgc cgtctgaaca tttcagacgg
       61 catttgctca aataaacctt atcggctgtt tattcagtcg gatttatttt ttcttttgcg
      121 gaggaaggtc ggtacaagta cccaatgcca cttccgccgc cataccgatg gattcgccca
      181 aggtcgggtg cgggtggatg gttttgccga tgtctgccgc gtcgcagccc atttcgatgg
      241 caaggcagac ttcgccgatc atgtcgccac cgttcggacc gacaatgccg ccaccgatga
      301 tgcggccggt ttcggcatcg aaaatcagct tggtaaagcc gttgtcgcaa ccgttggcaa
      361 tcgcacggcc ggaagccgcc cacgggaagt tggctttggt gattttgcgg ccggatgctt
      421 tggcggccaa ctcggtttcg cccacccacg ccacttcggg ggaagtgtag gcaacgccag
      481 gaatcacgcg cgcgtcgaag taggctttgt ggccggcgca gttttcagca gcaacgtggc
      541 cttcgtgaac ggctttgtgt gccaacatcg gttgaccgac gatgtcgccg atggcgtaga
      601 tgtgcggcac attggtacgc atttgtttgt ccacttcgat gaagccgcga tcggtaacgg
      661 caacgcctgc tttttccgcg ctgatgagtt tgccgttggg cgcgcggccg gcggcaacca
      721 atacggcatc gtagcgttgc ggctctttag gcgcgttcgc gccttcaaag gtaacgtaaa
      781 cgccgtcttc tttcggctca actgcaacgg ttttggtgtt gaccataatg ttgtcaaaac
      841 ggtattcgtt ttgtttttgc catactttta ccaaatcgcg gtctgcgcct tgcatcaggc
      901 cgtccatcat ttcaaccaca tccaaacgcg aacccagcgt gctgtaaacc gtacccatct
      961 cgaggccgat aatgccgccg ccgataatca gcagtttgcc cggtacttct ttcagagcca
     1021 atgcgccgct ggaatcgatg atgcgcggat cttcaggaat gaaaggcagt ttggttacgc
     1081 ggctgcctgc tgcaatgata cagtttttga aggcaacgat ttttttctcg ccggtagggg
     1141 ctgcctgttc gtacgcgtcg ccggcagtca gcgacacttc caagtggtgc ggatctaaga
     1201 attgcccgtc gccttggata acgtctactt tgcggccttt agccatgcct gccaaaccgc
     1261 tggtcagacg ggaaaccacg ccgtctttgt aggcgcgaag catatcgatg tcgagttccg
     1321 gctcggggta tttgataccg ttggcagcca agtggcgcac ttcgtcgata acggcggcat
     1381 tgtgcaacaa ggctttggaa gggatacagc cgacgttcag gcaaacgccg cccaaagttt
     1441 tgtaacgttc gacgatggcg actttcaagc cttcatcggc agcggcaaat gcagcggagt
     1501 aaccgccggg gccgccgccc aatacgacca cgtcgtactc ggcatcggca gaaccgccga
     1561 attgcgcggc ttgcggggca ggagcagcag ctttaggggc ttcttgcgcc ggggcggcag
     1621 ctgcttcggc tttaggggcg gcagccgcac cttcggcttc gacgacgaca atcaaaccgc
     1681 cttcggagat tttgtcgccg actttgactt tcacttcttt gaccacgccc gcaacttcgg
     1741 caggcacgtc catcgtcgct ttgtcggttt ccaaggtaat cagggtgtcg tcaacggcga
     1801 tggtgtcgcc cacgttcact tcaactgcga taatatctac attttcgtgt ccgccaatgt
     1861 cgggcacttt caattcaact aaagccatta tattgacctt tcaacttagt taatttctat
     1921 caactcttta gcaataatca cttcttcagg aatacagcca caatcttcag ctctgattgt
     1981 tacttccttc tcatttgggt tttctggatg ttcaacctca tattcaaaaa tagaatattg
     2041 accaaacaaa tccctattta aaacatatat atagccattt tcgatgccgg aacttgttgc
     2101 aaatttcttg gcaatttcct tgtctgttgt cgtagatata taacatccgt catatagacc
     2161 tgtttcaatt tgatgggcgt aaactgcatt cttcacactt ggaccatgtg tagctttacc
     2221 atcatattta aacttaccat cataacgaat tgcaacttca gctttattac ctttaggttt
     2281 taactgtcca ttattttgct catcttgttg gcaactaatg cctctatata agaaatttcc
     2341 cataagttat taatttaaag gttgaattta acaccattac tcatcaatct caagcaatcc
     2401 aaacattatc agactgcttg agagggatgt tttattttat aaggtaatgc ggcggaagtc
     2461 tttcaacagc ttcgccaaga atacggtgaa gcgcataccg gccgcaccgt cgatgacgcg
     2521 gtggtcgaag gacaggctca acgggcacat caggcgcggg gcaaactctt tgccgttcca
     2581 aaccggtttg atttgggatt tgcacacgcc caagatggcg acttcgggag cgttcacaat
     2641 cggcgtgaaa cctgtgccgc cgatgccgcc caagctggaa atggtaaagc acgcgccttg
     2701 catttcttgc ggtttgagct tgccttcgcg ggcttttttg gacaattcgg tcaattcttg
     2761 gctgatttgt ttcaagcctt tttgatctac gtctttgatg acgggaacaa ccaagccgtt
     2821 cggcgtatcg gctgcgaaac cgatgttgaa gtagtttttc agcaccaggt tgtcgccatc
     2881 cagagaagcg ttgaattcgg ggaaggcttt cagcgcggaa acggaggctt tgatgatgaa
     2941 cgccagagga gacagtttca cgccttcgcg ttcccattct ttgttcagtt gtttgcggaa
     3001 ttcttccagt tcggtcatat ccgcttcttc gtgtacggta acgtgcggaa tcacaaccca
     3061 gttgcgggac aggttttgac cggaaatttt cttaatgcgg gacaattctt taacttcgac
     3121 attaccgaat ttggagaagt ccactttagg ccagggcagc aagtccagac cgccgcccaa
     3181 agatgcgccg gctgcggcag gttttgccgc accgccctgc attacggatt tcacaaaggc
     3241 tttgatgtcg tcgcccacga tgcggccttt caagccggta cctttgactt ggcccaaatc
     3301 cacgcccaat tcgcgcgcca gtttgcgtgc ggaaggaccg gcgtgtgctt tggcgaaagc
     3361 ggcctcgtcg attttggcag cggcaggtgc agaaggtgca ggcgcggcga cagcagtcgg
     3421 agccggggcg gcagccggtg cgggtgcggc ggcttgagca ggggctgctg cggcagagcc
     3481 ggcggtttct acttcgataa tggcagagcc ttcggacact ttgtcgccga cttttaagaa
     3541 tacggcttta acgacaccgg cagcggtaca aggtacgtcc attgtcgctt tatcggtttc
     3601 caaagtaatc agcgtgtcgt cttcggcaac ggtgtcgccc actttgattt caacggcgat
     3661 tacatccaca tcggtatggc cgccgatatc gggaacggct acttgaacgg ttgcaccgcc
     3721 tgcggcaacg ggtgcggcag caggtgcagg ttgtgcttcg gcagcagcag gagcggcttc
     3781 ggcagcggcg gcaccggttt caacggtcag aataacgccg ccttcagaga ttttatcgcc
     3841 cactttaact ttaacttctt taacaacacc ggctgcatca gcaggtacat ccatagtggc
     3901 tttgtcggtt tccaaagtaa tcagggtgtc gtcaacggca atagtgtcgc ctactttgac
     3961 ttctacggcg atgatgtcga cgttttcatg accgccgata tcagggactt tgatttctac
     4021 gatactcatt tgagttcctt taatattttc agtttgatgc cgtctgaaaa tttgtttcac
     4081 acggcatcgg tacagtttgt tcaaaccatt caagcattcg gttttcagac ggcctgatgt
     4141 ctgaatgaag caaacaggcc gtctgaaaca tcaattagcg tttccagcta ggagctgaat
     4201 cggctttgat accatatttt tcaatggctt gttgaacggt ttctttgctg actttgcctt
     4261 gatcggccaa tgcgctcaat gcagcaacgg caacgttgta gcggtcaact tcgaagaagc
     4321 tacgcaggtt ggcgcggctg tcggagcggc cgaagccgtc agtgcccaag acgtggtaat
     4381 cgttcgggat ataggcgcgg atacggtcgg catagctgcg gatatagtcg gtagcggcaa
     4441 tcactgggcc gtcatgacct tgcagttgag aagtaacaaa cggtactttt gcagtttcta
     4501 aaggattcag gcggttgaaa cgttctgctt cgacagcgtc gcgatgcaac aggttgaaag
     4561 atgggcaaga ccaaatgtct gcttccacac cgaagtcggc tttcagcagc tcggcaccgg
     4621 caatcacttc ttgcagaatc gtaccggaac ccatcagttg gactttcttg tcgcctttgc
     4681 cgccggcttt cagcaggtac atacctttga ggatgtcttg ttccgcacct tccggcatat
     4741 ccggatgggt gtagttctcg ttcatcaggg tgatgtagta gaacacgtct tcattattgg
     4801 catacatacg gcgcagaccg tcttgtacga tgacggcgac ttcgtattgg aaagtcgggt
     4861 cataagatac gcagttcgga atcaaatctg cttgaatatg gctgtggccg tcttcgtgtt
     4921 gcagaccttc gccgttcagc gtcgtacggc cggcagtacc acccaacagg aagccgcgtg
     4981 cgtgcatatc acctgccgcc caagccaagt cgccgatacg ttggaaaccg aacatagagt
     5041 agtagatgta gaaaggaatc atcgcaaagt tgctgttggc gtagctggtt gcagcagcga
     5101 tccagtcagc cattgcgccc ggttcgttaa taccttcttg caagatttga ccgtcaacgg
     5161 attctttata gaacatcagt tggtctttgt cttgaggggt atattgctga cctttcggat
     5221 tccaaatacc gtattggcgg aacatgcctt ccataccgaa agtacggctt tcgtcgggaa
     5281 cgataggtac gacgcgtttg ccgatttttt tgtctttcaa caaggttgac aaaatgcgga
     5341 caaatgccat agtggttgag aattcgcggt cgccgctgga tttcagttgg gtatcaaatg
     5401 cggacagctc cggcacttcc aaaacttctt gggtaggttt gcgttgcggc aggtagccgc
     5461 ccaaagcatc gcggcgtgcg tgcaggtatt tgtattcttc cgtatcgggg gcaaaagtca
     5521 ggtaaggcag atcgccgctt tcgatttgtt cgtcggtaac cggaatgtca aagcggtcgc
     5581 ggaattgttt cagggacgct ttgtccattt ttttggcttg gtgggcaacg ttctgacctt
     5641 cgccggatgc gcccataccg taacctttaa tggttttcgc caagatgacg gtaggtttgc
     5701 cgtcggcatg gttcgctgcg cggtcgtagg cgttgtacac tttttgcggg tcgtggccgc
     5761 cgcggttcaa tgcccagagt tgctcatcgg tcatatcggc aaccaatgct ttcagttcgg
     5821 gcgtattgaa gaagtgttcg cgcacatacg cgccgtcttt ggatttgtaa gtttggtagt
     5881 cgccgtccaa acattcttcc atacgttggc gcaggatacc gtctttgtct ttcgccaaga
     5941 ggcggtccca gcggcggccc caaatgactt tgacgacatt ccagccggcg ccggcaaagt
     6001 tgccttccaa ttcttggatg attttgccgt tgccgcgtac cggaccgtcc aagcgttgca
     6061 gattgcagtt gatgacgaaa atcaggttgt ccaagccttc gcgtgcagcc agtgcgattg
     6121 caccttgaga ttcgggttcg tccatttcgc cgtcgccgca gaaacaccat actttacggc
     6181 ctttggtttt tgccaaacca cgcgattcca agtatttcag gaaacgcgcc tgataaatcg
     6241 ccatgatggg ccccaagccc atggatacgg tcgggaactg ccaaaagtcg ggcaagaggt
     6301 gggggtgcgg ataggaaggc agaccgtgtc cgtccacttc ttggcggaag ttgttcagct
     6361 ggtcttcggt cagacggccc tcgacgaatg cgcgtgcata gatgcccggg gcgacgtgac
     6421 cttggaagaa gaccaaatcg ccttcttcgc ctccgccttt ggctttccaa aagtggttga
     6481 aaccgacttc gtacatggtg gcggcagatt ggaaagatgc gatgtgccca cccagttcca
     6541 aatctttctt gccggcgcgc aatacgatgg cggcggcgtt ccagcgcacg aatgcgcgga
     6601 tgcggtgttc gatgttttgg tcgcccggaa tgcctttttc gttttcaacc gaaacggtat
     6661 tcaaatacgg ggtggtcgtg ccgtgtggca tacgtacgcc cttgtcgcgg cagtatttga
     6721 ccaggttttc caagaggtat tgcgcgcgtt cgccgccttc atattcgagg acggagctta
     6781 acgcgtccag ccactcttgg gtttcgatag ggtcaacatc gtgtaattgg gtggacatag
     6841 tatctatcct ttatgttgag tgtttaatcg gcagggaggg tgtagccaac ccctttattt
     6901 tcgtttgtga aaaataataa gcaaaaacga aaatttctaa atcaaaccga aaaaaataat
     6961 ttaagtttaa tgttttcaaa aaattataaa agttttggaa acaagggaaa acttgaaaaa
     7021 aatgttcgat ctgctccaca cgaaatccta tcagcaatcg ggtctgctgg caaataacca
     7081 tgcctgtaaa agcaggtttt atactgaagt tggcgtttgg taaggtcaag tctttttgat
     7141 ttcgaaatat tctgtttgat tttggcgata ttttttcgga attgtggtat atcgaatcat
     7201 tatgtctctg tcggcaattt tcctattgta gaaaatctat acacaagctt tgtggataac
     7261 cgtgttgata agccacggct tgtgttaaaa aagtcttacg caacaaatgt acgcttattt
     7321 tgattaaaaa ataggcacat ttaaatctat aaaaatcatg tgcttatgtt caatatgatg
     7381 tttctggtca tgtttttgcc gtctgttatg ggatttgtcc cgcgcttggg gcagggggtg
     7441 aattttggag gttggtgcaa aacctgcttt tttcgcgtta caatctgcac ccgatttact
     7501 tttgaatctg cctgtttgga aaaatacttt tatgaaagcc agccaattct ttatctctac
     7561 tttaaaagaa gcccctgccg aagccgcgct tgccagccac aagctgatga ttcgcgccgg
     7621 cctgattaaa gccaacgcgt ccggccttta tacttggatg ccgatggggc tgcgcgtgtt
     7681 acgcaaagtc gaaaacgtcg tgcgcgagga aatggcgcgc gcgggcagcg tggagttgct
     7741 gatgccggtg gtgcagcctg ccgagctgtg gcaggaatcc ggccgctggg agttttacgg
     7801 taaagaactg ctgcgcctga aagaccgcca cgaccgcgat ttctgcatgg gcccgacctg
     7861 cgaggaagtc atcgccgaca tcgtgcgcaa agaaatcaac agctacaaac agctgccgaa
     7921 aaacttttac cacatccaaa ccaaattccg cgacgaagtg cgcccgcgtt tcggcgtgat
     7981 gcgcgcgcgc gagttcgtca tgaaagacgc ttattccttc catgccgact acgcctcgct
     8041 tcagacgacc tatgatgcca tgtatgacgc ttactgccgc atcttcactc gtttgggttt
     8101 ggcattccgc cccgtcgccg cagacaccgg cagcatcggc ggtacgggtt cgcacgagtt
     8161 tcaagtgttg gcggaaagcg gcgaagatgt tattgcgtac agcgacactt ccgattacgc
     8221 cgccaatatc gagttggcac cgaccttgcc gcttaaaggc gaacgcgccg ctgctcaggc
     8281 tgtgttgacc aaagtacata caccaaacgt caaaaccatt gagtctttgg ttgaattcct
     8341 gaatattccg gttgaacaaa ccctcaaatc catcgtggtc gaaggcgaaa acgaaggcga
     8401 aatcgtctta ttactgttgc gcggcgacca tgagttcaac gacatcaagg cagaaaaact
     8461 ggcgggcgtg aaatcgccat tgacgatggc aagttctgcc gcgattgtcg aacagttcgg
     8521 cgcaaacggc ggctcgctgg gttcggtcgg ctttgcaggc aaagtttatg ccgatttcgt
     8581 cactgaaaaa ggcgcggact gggtcatcgg tgcaaacgaa gacggctacc actacaccgg
     8641 cttcaacttc gggcgcgacg ctgccgagcc tgaatttgtc gatttgcgca atgtcgtcga
     8701 aggtgacgaa agccccgacg ggcaaggccg tctgaaactg gcgcgcggca tcgaagtcgg
     8761 acacgtcttc cagttgcgcg acaaatacac ccaagccatg aacgtgagct tcctcgacaa
     8821 caacggcaaa tcgcaaatca tggaaatggg ctgctacggt atcggcatca cccgcgtcgt
     8881 tgccgccgcc atcgagcaga acaacgacga aaaaggcatc atctggacga aggcgatggc
     8941 gccgtttgaa gtcgttatcg tgccgatgaa ctacaaaaaa tcagacgccg tgcgcgaagc
     9001 cgccgacaaa atctatgccg aattgctggc ggcaggcgcg gatgtgctgc tggatgaccg
     9061 cgacgaacgc gcaggcgtat tgctgaacga ttccgagctg ttgggtattc cgcaccgcat
     9121 cgtcatcggc gaccgcgctt tgaaagaagg caatgtcgaa tacgccgaac gccgcaacaa
     9181 cgaagcgcag gcagttgcaa tcggagaaat tgttgcgcgt gtaacagctt cattaaatgc
     9241 gtaagcttgc tcgaatgccg tctgaaaata atttcagacg gcatttttac ttagggggaa
     9301 agtgaagtgg cttcagtatt tttagaagga gaggcagtcg aggtcggcaa tattttctgt
     9361 atcggcagga actatgccgc acatatcgaa gagttaaaaa acgaaatacc ttccgaacct
     9421 gtggtgttta tgaagccgtc aggcagcatt ttgaacagcg gcggtacaat cttgctgccc
     9481 gagtttagta gggatgtgca gtttgagtgc gaactcgtgc tgctggtcgg taaggattca
     9541 gacggcacgg gcgagggtaa agatattttg ggatgtgtcg caggatatgg tgtagggctg
     9601 gaccttacgg cacgggatat ccaatgccgt cggaaggaaa aagggctgcc ctggctgaag
     9661 gcaaaagggt tcaggcattc cgcgtgcgtg tcggattttg cggcggcagg cagaatcggc
     9721 aatcccgaga aggtgttgtt ttcattaaaa caaaacggcg tattgaaaca gcgcggggat
     9781 accggtttga tgatttaccc gattcgggaa attttacaca aactggcggc agattacggt
     9841 ttggggaaag gggatttggt gtttaccgga acgccctccg gcgtgggcgc aatcggtgcg
     9901 ggggacaatt tggcattgga gctggacggc ttggtgcgcg cctctttcac ggtagggtgt
     9961 taaccgcgcc gcttaaccct cacgcccgtt gaaacattcc gccggaccgc cctcgaaaaa
    10021 accgtgcaag atggcttgcg ccgccacttg gtcgagcacc gatttgcgtt ttttgccgaa
    10081 gacctgtgct tccgaaagca ggctttcggc atagacggac gacagccgtt cgtcaaccca
    10141 atagacgggg agattgaacc tgccgttcag cctgcgtccg aacttgcgcg acaggtgcgt
    10201 catttcatgt ttcgtgccgt cggtatgcac gggcagtccg acgacaaaat aacgcggctg
    10261 ccattcttga accagcttgg cgattgccgc gaacttttca tcgttgctgc cgccggtaac
    10321 ggtgctcaaa ggatgggata gccctaattc cgcgtctcct tgtgccacgc cgatacgcgc
    10381 ttcgccgaag tcgaatgcca gtgccgttcc ttttggaatt ttatgcatgg ccggctcccg
    10441 aaaacagggc gagcgggtcg atgccgagtt tggcgaatgc ggcggcgtaa cggtgttcgt
    10501 aggggatgtc gaacaggatg tgttcgtcgg cgggaacagt cagccacgca ttgtcggcaa
    10561 gttcgcgttc gagctggcct tttccccagc ttgaatagcc tatgctgatc aaggctttgt
    10621 caaccgcacc ttcgcgtgaa atattttcaa tcacgtctcg ggaagaagtt agcgcgatat
    10681 tgtctgaaac gccgatactg ctttgccagt tgccgatcgg ggtatgcacg acataacctc
    10741 gctcgacttg caccggcccg cccatcatca cgctgtcgtg ctgcatccgc atggggatgt
    10801 ttttgccggt ggcggaaaaa atcatgtcca tcgtaatcgg agagggtttg ttgatggcga
    10861 tgccgagtgc gccgtcttca tcgtgtttgc agatatagac gaccgattgt gaaaaaaacg
    10921 cgtcttccat atcgggcatg gcgacgagaa agtggttgga gaggttcata tcagcgtttg
    10981 tcgggatttt ctatcgggtt ggaaatcatg caggcgccgg caagcaggtt gccggaagta
    11041 tcggcttgca ccccatcggt ttgcggtatg ccgccgccga aacaggaaac cgcgcttaag
    11101 gaatttttcg gcgagccttg gatgatttta tcgctgtaca ccaaataggc gaaggttttg
    11161 cgtttggggt cgtaataacg gacaatctgc cggctcttga acgcgaagct cgcaccgtgt
    11221 ttgaaaactt ctttcggttt gcgcacggcg gtttcgtcaa aagaaatcga agatgccgtc
    11281 tgaacgcacg aaaccgatgc gtcggacgcg tcctcttcca aattgaccat ttccttcaag
    11341 ccgccttttt ttgcatacga aatataacag gcaacccctt gaacgtcggg atcgtcgaat
    11401 ccttccactt cgatacggtc gtttttgccc agtatgttga aaacggtact tgcccgtccg
    11461 attttatcgg tttcgccgct gccgcaggca gtcagcagga cggcggcaga cagcagtagc
    11521 agtctgttca tcaaaatttc cttatcggtt gaatccccgt tttccgtgaa ggcggcaggg
    11581 cttgctttgt ttggaggggc gcacgttccc aaaatcgggc aacatacggt atgcggctta
    11641 atgtgctacc ctgtgtgtcg gaacacaata tccgttgcgg cattgaaaat atgacgcaca
    11701 atataaggct gtttgagccg attgcaagta tccgcgcaaa gaaggcgcaa gattgggcaa
    11761 acggcatttt cagacggcat aactgacagt ataatccaaa cgtccggctg ctttcgtgca
    11821 gccttcagta ttatccaact ttttccgaag gtcgaaccat gcgctacaaa ccccttctgc
    11881 ttgccctgat gctcgttttt tccacgcccg ccgttgccgc ccacgacgcg gcacacaacc
    11941 gttccgccga agtgaaaaaa cagacgaaga acaaaaaaga acagcccgaa gcggcggaag
    12001 gcaaaaaaga aaaaggcaaa aatggcgcag tgaaagataa aaaaacaggc ggcaaagagg
    12061 cggcaaaaga gggcaaagag tccaaaaaaa ccgccaaaaa ccgcaaagaa gcagagaagg
    12121 aggcgacatc caggcagtct gcgcgcaaag gacgcgaagg ggataagaaa tcgaaggcgg
    12181 aacacaaaaa ggcacatggc aagcccgtgt ccggatccaa agaaaaaaac gcaaaaacac
    12241 agcctgaaaa caaacaaggc aaaaaagagg caaaaggaca gggcaatccg cgtaagggcg
    12301 gcaaggcgga aaaagacact gtttctgcaa ataaaaaagt ccgttccgac aagaacggca
    12361 aagcagtgaa acaggataaa aaatacaggg aagagaaaaa tgccaaaacc gattccgacg
    12421 aattgaaagc cgccgttgcc gctgccacca atgatgtcga aaacaaaaaa gccctgctca
    12481 aacaaagcga aggaatgctg cttcatgtca gcaattccct caaacagctt caggaagagc
    12541 gtatccgcca agaacgtatc cgccaagagc gtatccgtca ggcgcgcggc aaccttgctt
    12601 ccgtcaaccg caaacagcgc gaggcttggg acaagttcca aaaactcaat accgagctga
    12661 accgtttgaa aacggaagtc gccgctacga aagcgcagat ttcccgtttc gtatcgggga
    12721 actataaaaa cagccagccg aatgcggttg ccctgttcct gaaaaacgcc gaaccgggtc
    12781 agaaaaaccg ctttttgcgt tatacgcgtt atgtaaacgc ctccaatcgg gaagttgtca
    12841 aggatttgga aaaacagcag aaggctttgg cggtacaaga gcagaaaatc aacaatgagc
    12901 ttgcccgttt gaagaaaatt caggcaaacg tgcaatccct gctgaaaaaa cagggtgtaa
    12961 ccgatgcggc ggaacagacg gaaagccgca gacagaatgc caaaatcgcc aaagatgccc
    13021 gaaaactgct ggaacagaaa gggaacgagc agcagctgaa caagctcttg agcaatttgg
    13081 agaagaaaaa ggccgaacac cgcattcagg atgcggaagc aaaaagaaaa ttggctgaag
    13141 ccaggctggc ggcagccgaa aaagccagaa aagaagcggc gcagcagaag gctgaagcac
    13201 gacgtgcgga aatgtccaac ctgaccgccg aagacaggaa catccaagcg ccttcggtta
    13261 tgggtatcgg cagtgccgac ggtttcagcc gcatgcaagg acgtttgaaa aaaccggttg
    13321 acggtgtgcc gaccggactt ttcgggcaga accggagcgg cggcgatgtt tggaaaggcg
    13381 tgttctattc cactgcaccg gcaacggttg aaagcattgc gccgggaacg gtaagctatg
    13441 cggacgagtt ggacggctac ggcaaagtgg tcgtggtcga tcacggcgag aactacatca
    13501 gcatctatgc cggtttgagc gaaatttccg tcggcaaggg ttatatggtc gcggcaggaa
    13561 gcaaaatcgg ctcgagcggg tcgctgccgg acggggaaga ggggctttac ctgcaaatac
    13621 gttatcaagg tcaggtattg aacccttcga gctggatacg ttgatcggat tgccgaaacg
    13681 gtaggcttta tgcaaatcgt gccgatatgg tgcgctgttc cgtgaagttt gccgtacctg
    13741 tattttccgg tttttggaaa tcaagagaga aagttaaaga agaatgtcga aacctgtttt
    13801 taagaaaatc gcactttata ctttgggtgc aatcagcggc gtggccgtca gtctggcggt
    13861 gcagggtttt gccgccgaga aggaccggcg ggataacgaa gtcctgccgg tgcaatccat
    13921 ccgcacaatg gcggaggttt acggtcaaat caaggcaaac tactatcagg acaaacccga
    13981 tgccgatttg tttgaaggtg cgatgaaggg tatggtggcc ggtttggatc cgcattccga
    14041 atatatggat aaaaaaggtt atgccgagat aaaggagtcc accagcggcg aatttggcgg
    14101 cttggggatg gaaatcgggc aggaagacgg atttgtcaaa gtggtttcgt cgattgagga
    14161 cacgcctgcg gaacgggcgg gggtgaaaag cggcgatttc attgtgaaaa tcgataatgt
    14221 ttcgacgcgg gggatgacag tcagcgaagc ggtgaagaaa atgcggggca agccgggtac
    14281 gaagattact ttgacgctgt cgcgcaaaaa tgccgacaag ccgatagtcg tcaacctgac
    14341 ccgtgccatt attaaagtga aaagcgtccg ccatcacctg atcgaacccg attacggcta
    14401 tatccgcgtg tcgcagttcc aagagcggac ggtcgaaagc gtcaataccg ccgcaaaaga
    14461 gctggtaaag gaaaataaag gaaaaccgct caaggggctg gtgttggatt tgcgcgacga
    14521 ccccggcggg cttttgaacg gcgcggtcgg cgtgtcggcg gcgtttttgc cgcctgatgc
    14581 gctcgttgtc agtacgaaag ggcgggaagg tacggaaggc tttgcgttaa aggcgcgtcc
    14641 cgaagattat atttttaaag ccggcacgga tcctttggcg ggtattcctg ccgagttgaa
    14701 aaccataccg atgacggttc tgattaattc gggttcggct tccgcgtcgg agattgtcgc
    14761 aggtgcattg caggatcata aacgcgcggt tatcgtcggt acgcagagct tcggcaaagg
    14821 ttcggttcag actttgattc ctttgtccaa cggcagcgcg gtcaagctga caacggcact
    14881 gtattatacg ccgaacgacc gttctattca ggcgcagggg attgttcccg atgtcgaagt
    14941 aaaagataag gaacgcattt ttgaaagccg cgaggcggat ttggtcggac acatcggcaa
    15001 tcctttgggc ggcgaggatg tgaacagtga aacccttgcc gtgccgcttg aaaaagatgc
    15061 ggataagccc gctgtaaaag aaaaaggtaa aaagaaaaag gacgaggatt tgtcttcaag
    15121 gcggattccc aaccctgcca aagacgacca gttgcggaaa gctttggatt tagtcaagtc
    15181 gcccgagcag tggcagaagt ctttggggct ggcggcgaaa aagccggttt caaataaaga
    15241 taagaaagat aaaaaagata agaagtaggt tttgacgatg ccgtctgaaa ggatttcaga
    15301 cggcatatta tgtttgaaag gacaggttat gagcgatttt ttaccggaat ttgaaccgtg
    15361 cggcatttat agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagtacaa
    15421 atagtacggc aaggcgaggc aacgccgtac tggtttttgt taatccacta tacttgggta
    15481 gcgattgggt ttcagacggc atacattcag gatttcaccg ccttatgccg tctgaaagac
    15541 gtttccgacc catccctact acacgctttg tccgcaaacg catcacaggc aagattatgg
    15601 aagtcatccg ataccccgat tcccctttca aactccacca acccttcccg cccgcaggcg
    15661 accagcctac cgctattgcc ggcttgctcg aagggctttc agacggcctt gcctaccaaa
    15721 ccctgctcgg cgtaaccggt tcgggcaaaa cctacaccat ggcgaacgtc atcgcccaaa
    15781 gcggccgacc cgccatcatc atggcgcaca acaaaaccct tgccgcccag ctttatgccg
    15841 aaatgcgcga gtttttccct gaaaacgcgg tggaatattt cgtctcctac tacgactatt
    15901 accaacccga agcctatgtg cccagccgcg acctgttcat tgaaaaagac agcgcgatca
    15961 acgaacatat cgagcagatg cgcctttccg ccaccaaaaa cctgatgacg cgcgatgacg
    16021 tgattatcgt tgccactgtg tccgccattt acggtatcgg cgacccgact gagtatcaac
    16081 aaatggtgtt gtccgtcaaa gaaggcgaca ccatcgagca gcgcgacatc atcgccacgc
    16141 tcgtttccat gcagtacgaa cgcggcgatt tggatttcaa acgcggcagc ttccgcgtgc
    16201 gcggcgacgt gattgacgtg taccccgccg aaagctccga aaacgccttg cgcatcagcc
    16261 tgtttgacga cgaaatcgac cgcctcgata tgttcgaccc gctttcaggc agcctgcacc
    16321 aacgcgtcgg ccgctacacc gtcttcccgt ccagccacta cgttaccccg cgcgacaccg
    16381 tattgcgcgc ctgtgactcc atcaaagaag aattgcgcga acgcattgat ttctttacca
    16441 aagaaaaccg tcccgtcgaa caacaacgca tcgaacagcg cacccgcttc gacctcgaaa
    16501 tgctctacga aatgggcttc tgtaaaggaa tcgaaaacta ctcccgccac ttctccggca
    16561 aaaaagaagg cgaaccgccg cccacgctga tggactacct gccaagcaac gccatcatgt
    16621 tcatcgacga aagccacgtt accgtaaccc aaatcggcgg tatgtacaaa ggcgacgcat
    16681 cgcgcaagca aaacctcgtc gattacggct tccgcctgcc ttccgcccgc gacaaccgcc
    16741 cgctcaaatt ccacgaattt gaaaaagtca tgccgcaaac cgtcttcgtt tccgccaccc
    16801 ccgccaaata cgaagaagaa cacgccggac aagtggtcga acaagtcgtc cgccccacag
    16861 ggctggtcga ccctcaaatc atcatccgcc ccgtcgccac ccaagtcgac gatttaatga
    16921 gcgaaatcaa cgaccgcatc caaaaaggcg aacgcgtact cgttaccacc cttaccaaac
    16981 gcatggcgga gcaactcacc gactattaca gcgaactcgg catcaaagtg cgctacctgc
    17041 acagcgacat cgacaccgta gagcgcgttg aaattattag agatttacgg ctcggattgt
    17101 ttgacgtact cgtcggcatc aatctcttgc gcgaaggcct cgacatccca gaagtctccc
    17161 tcgtcgccat cctcgacgca gacaaagaag gcttcctgcg ctcccaccgc agcctgattc
    17221 aaaccatagg ccgcgctgcg cgcaacgtga acggcgtcgc gattctgtac gccgacaaaa
    17281 tcaccgactc tatgaaagcc gccatcgacg aaaccgaacg ccgccgtgaa aaacaaatta
    17341 aattcaacga agaacacggc atcgtgccgc aacagattaa aaaacaggtc aaagacatca
    17401 ttgacggcgt gtaccacgaa gaagacagcg gcaaaggccg tagacaaggc aaaaacaaag
    17461 ttaaagtcgg cgaaattcac aacgaagaag acgcgattaa agaaatcgcc aaactcgaaa
    17521 aagccatgca acaggcggct agggatttgc agtttgaaga agcggcggtg ttgagggata
    17581 ggattaggaa tattaaagag aatttgttgt ttggggcaga gtaaaggagc ttgatttttt
    17641 aattagcgaa gatacaataa cgtcatctat tatttttata ggaaagtaac tatggcagag
    17701 caactacagc tgattcctcg tgttgaaagc ggtgaaatta tcccacagag aatgagtgac
    17761 ggatatatca atgcgacagc attgtgtaaa tctgtaggta aatcttacag tgattataga
    17821 caattacagt ctacaaatca tttcttaaat gaattgaaag cctagacggg attatccgaa
    17881 caacaattga ttcaacagag aattggtgga gaaccttctt tacagggctc ttgggtacac
    17941 ccttatttag caattaattt agcacagtgg ttatcccctg catttgctgt aaaggtatct
    18001 acttgggttc atgaatggat gtcaggtaaa gtcaatgctt ctccttccca ttccgcgcat
    18061 atccaaagat acttgcaaaa tagaggaaaa attccatata cgcatttttc aatgcttaat
    18121 gaaattacgc taaatttaat tgctcctttg gaggaacaag gttatgtatt acctagtcag
    18181 ttagttccgg atatttcaga aggaagatta ttttgtagat ggctgaggga aaatagaaac
    18241 gttgatccga ataacttccc aacatataaa catgaattcc ttgatgctcg gagacccgtg
    18301 gaggccaaat tatatccaat agaatactat caagatttta aatggcactt taatgaagta
    18361 tggttaaaag aaaaagctaa acaatatttt tcagaacgtg atgcagcaat tttgccttat
    18421 ttaaccccgt taatgttacc agcaagcgag tcgtagcaag ccgtagcctg cattaccaac
    18481 caccgcgtgc gtagctacgc catacaccct acctgcgggc gggacaaact ttaaggggct
    18541 gtactagatt agccctaaat cccataccaa tcccgcagga ttttaagctg ttgagacggt
    18601 gtgccgaagt taaatcgaaa ttagcattct ttcaagaaca gtggaaaaga tttgcaatcg
    18661 attccgttgt attttcgcaa gacgcgtttt gcctgattcc aaaaattctc aatgccgtta
    18721 atgtggttct gacggtctgc aaattccttg gaatggttga tgcggtaatg gataaaaccg
    18781 ctcacgtcca acttgtcg
//
LOCUS       DAWFLT010000033        17071 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWFLT010000033 DAWFLT010000000
VERSION     DAWFLT010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17071
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.033"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(27..653)
                     /locus_tag="WGR74_001149"
     CDS             complement(27..653)
                     /locus_tag="WGR74_001149"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ0905575.1"
                     /translation="MRKIRTKICGITTPEDASAAVAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDTFCRQFDRPYIK
                     AIRVQTASDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
     gene            complement(712..1203)
                     /gene="greB"
                     /locus_tag="WGR74_001150"
     CDS             complement(712..1203)
                     /gene="greB"
                     /locus_tag="WGR74_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ0905576.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            complement(1303..2847)
                     /gene="purF"
                     /locus_tag="WGR74_001151"
     CDS             complement(1303..2847)
                     /gene="purF"
                     /locus_tag="WGR74_001151"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ0905577.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLVGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALTYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANERSAAEIAAEIGADGIVFQNLSDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            complement(3056..3553)
                     /locus_tag="WGR74_001152"
     CDS             complement(3056..3553)
                     /locus_tag="WGR74_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ0905578.1"
                     /translation="MNNLPVADLLVFAIIAACIVISTMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKILRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGILIITLLVMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAE
                     TPEDD"
     gene            complement(3546..4544)
                     /gene="ftsN"
                     /locus_tag="WGR74_001153"
     CDS             complement(3546..4544)
                     /gene="ftsN"
                     /locus_tag="WGR74_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0905579.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            complement(4557..5831)
                     /gene="folC"
                     /locus_tag="WGR74_001154"
     CDS             complement(4557..5831)
                     /gene="folC"
                     /locus_tag="WGR74_001154"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ0905580.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DFDHQAFLGDTVEQVGFEKAGVFRGGKPAICGQNPAPASLLSHAEAIGAKLLMVQRDF
                     EFHALENIQWNYRFRPQHSDSPARNRNSLPFPALRGAYQLSNAACALTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            complement(5863..6309)
                     /locus_tag="WGR74_001155"
     CDS             complement(5863..6309)
                     /locus_tag="WGR74_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ0905581.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKEKTAKAAALSGSSMVTPYALF
                     LQWDTGGKTVRHCITHDMADPESYRRLKVWVLWRQPKKTAETDTPS"
     gene            complement(6314..6673)
                     /locus_tag="WGR74_001156"
     CDS             complement(6314..6673)
                     /locus_tag="WGR74_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905582.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            complement(6746..7474)
                     /locus_tag="WGR74_001157"
     CDS             complement(6746..7474)
                     /locus_tag="WGR74_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0905583.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            complement(7647..8426)
                     /gene="rsmA"
                     /locus_tag="WGR74_001158"
     CDS             complement(7647..8426)
                     /gene="rsmA"
                     /locus_tag="WGR74_001158"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ0905584.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVDRMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGRADDFEYFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            8623..9333
                     /locus_tag="WGR74_001159"
     CDS             8623..9333
                     /locus_tag="WGR74_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ0905585.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFGDADYFKVPSNGEAAHS
                     EKAFDAEKIKAFLHERGIASCPTVLKAVRQHGNASDKFLKVVETVDLAAVLAKIPECR
                     HICTTGGKATEILLDIQGGGIKMPKTGETVLFPFAGRDLTLTRLPSTSRTYPLSLAKK
                     AAAYRAFFEMAGLCEKQL"
     gene            9416..10618
                     /gene="trpB"
                     /locus_tag="WGR74_001160"
     CDS             9416..10618
                     /gene="trpB"
                     /locus_tag="WGR74_001160"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ0905586.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALQELADAYKAAKNDPE
                     FWAEFRCDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(10797..16271)
                     /gene="iga"
                     /locus_tag="WGR74_001161"
     CDS             complement(10797..16271)
                     /gene="iga"
                     /locus_tag="WGR74_001161"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ0905587.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADKIKQRDNAGTIKGNGEHHWNITFG
                     TNSKIGSTAVRLAGNEKDANNGQNVTFEDNGTLVLDQNINQGAGGLFFKGDYTVKGIN
                     NDITWLGAGIDVTDGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVI
                     LNQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFE
                     HIRNVDEGARIVNHNTSHASTITLTGKSLITNPNSLSVHSIQNDYDEDDYSYYYRPRR
                     PIPQGKDLYYKNYRYYALKSGGSVNAPMPENGVTENNDWVFMGYTQEEAKKNAMNHKN
                     NQRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLS
                     GRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKAAEIAVNQSASFSSGRNV
                     SNITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALNSFGATQINGN
                     VNLNQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNN
                     ASDAQSANQYHTLKINHLSGNGHFHYLTHLAENLGDKVLVKESASGHYQLHVQDKTGE
                     PNQEGLNLFDASSVQDRSRLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRV
                     KPAPSPATNTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKR
                     QQAEAERKSAKLAKQKAEAEREARELATRQKAEQERSSAELARRHEKEREAAELSAKQ
                     KVEAEREAQALAVRRKAEAEEAKRQAAELARRHEKEREAAELSAKQRVGEEERRQTAQ
                     SQPQRRKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELVRRQQEERKAAEL
                     LAKQRAEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELSANQKAAAEAQA
                     LAARQQKALARQQEEARKAAELAVKQKAETERKTAELAKQRAAAEAAKRQQEARQTAE
                     LARRQEAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQPQRRKRRAAPQD
                     YMAASQNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDALEDE
                     NVSESVDTSDKQPQDNTELHEKYENDYEEIPLDALEDEDVSESVDTSDKQPQDNTELH
                     EKVETVSLQPRAAQPRAQAAAQPQAQAAAQPQAQAAAQADAVSTNTNSALSDAMASTQ
                     SILLDTGASLTRHIAQKSRADAEKNSVWMSNIGYGRDYASAQYRRFSSKRTQTQIGID
                     RSLSENMQIGGVLTYSDSQHTFDQASGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLR
                     SRLQTQQKANFNRTSIQTGFTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKV
                     SSMAVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGNSFVYKADNQQQ
                     YSAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
BASE COUNT         3468 a         4187 c         4646 g         4770 t
ORIGIN      
        1 tcattccggc ggcaattttt gttgctttaa cgggataggc ggttggcggt tgcgataaag
       61 gcggcgactt tggctgggtc ttttttgcct ttagacgctt ccacgccgcc ggatacatcg
      121 accgcttccg ctccggtgat gcggacggct tcgccgacgt tttcaggggt cagcccgccg
      181 gcaagcaccc acggtttgcc cgaatattcc gccagcagcg tccagtcgaa gcggtgtccg
      241 gtgccgccgt attccgaagg gtggtaggca tcgaacagca gtgcctgagc gtcggggaag
      301 cggtcggcgg cgtttcggat gtctgatgcc gtctgaacac gaatggcctt gatatagggg
      361 cggtcgaact ggcggcagaa tgtgtcgtct tcgtcgccgt ggaattggat gatgtgtatc
      421 ggcacttcgg caaggatgcg gcggatgttt tgcgcgcttt cgttgacgaa aagggcgaca
      481 acgctgacaa acggcggcag tgcggcggtg atttttttgg cgcgggcaat atcgacggcc
      541 cggctgctgc cttggaaaaa gaccagcccg acggcatctg cacctgccgc tacggcggcc
      601 gatgcgtctt ccggtgtggt gatgccgcag attttggtgc ggattttcct cattcggtat
      661 tcctttattt gggaaacgat gcgtttgcac cgtttcagac ggcattcccg atcagtcgat
      721 tttgatgtat tcgacagaaa ggatttcaat ttcctcacgc ccttccggcg tgttcaaaac
      781 cacttcgtcg ccttcgcgcg ctttaatcag gcaacgcgcc aacggcgaaa tccaagaaat
      841 tttgttttgc gcggtatcga tttcatcgat gccgacgatt ttgacggttt gctcgcgccc
      901 gtcgccgcgc aacaggccga cggtcgcgcc gaaaaatact tggtcggtcg cttcgcgcaa
      961 ttcgggatcg acgacgacgg cggactccaa acgcttggtc aggaaacgga tgcggcggtc
     1021 gatttcgcgc atacggcgtt tgccgtaaag atagtcgccg ttttcgctgc ggtcgccgtt
     1081 gcctgccgcc cagttgacga tttggacgat ttcggggcgt tctttgttga ccagttgata
     1141 aagctcgtct ttcaatgcct gccatccggc gggcgtaatg tagtttttgg tttcggtact
     1201 catattgtgt gcggatgaaa cgggaaatgt gatgccgata tgggaaatgc cgtctgaaaa
     1261 cccggcgttc ggatttcaga cggcatcgcg gtttgggaag ccttattctt cgtcgcccgc
     1321 atcgctgatg ctgatgctgt gttccatcct gctcgggtgg attttcagac cgccgcagcc
     1381 ggatttctcg gcagacaggc ggtcgaggta ggcatcatcg atgtcgccgg tctgataaat
     1441 gccgttgaaa caggacgaat cgaaggattc gattttcgga ttgagtgctt tgacgacggc
     1501 ttccaaatcg ctcaaatttt ggaacacgat gccgtctgcg ccgatttcgg cggcgatttc
     1561 cgccgcgctg cgctcgttgg caatcaactc ttcgcgcgtg ggcatatcaa tgccgtacac
     1621 attgggatag cgcacttcgg gcgcggcgga ggcgatatag actttgcgcg cgcccgccgc
     1681 gcgtaccatt tcgacgattt cgcggctggt cgtcccgcgc acgatggagt cgtccaccag
     1741 caacacgctt ttgcctgcaa attcggtttc catcgggctg agtttttggc gcacggattt
     1801 tttgcgcgtc gcctgtccgg gcataataaa ggtgcggccg atatagcggt ttttaatcaa
     1861 accctcgcgg tagggtttgt cgagatggac ggcaagctcc atcgcgctgg ggcggctggt
     1921 gtcggggatg ggcatcacaa cgtcgatgcc gtccacgggc agctcgcgtt tgattttttc
     1981 cgccagcgac acgcccatat ccaagcgcga ttggtaaacg gatacgccgt caatcacaga
     2041 gtcggggcgg gcaaaataaa catattcaaa aaggcagggg ctgagtttgg cacggtcgct
     2101 gcattggcgg gcaatcattg tgccgtcaaa gccgacaaat accgcttcgc ccggccggat
     2161 gtcgcgttcc aaatcgtagg taagcgcgtt gaaggcgacg gattcggagg cgacggcata
     2221 ggattttctg ccttcgctgt cggtttgcga acccaatacc agcgggcgga tgccgtaagg
     2281 gtcgcggaag gcaagcatac cgtagcccgc aatcatggca accacgccgt atgcgccgcg
     2341 caccaggcgg tggacttcgg caacggcgtt gaaaatattg tcggcattga gccggtgcgg
     2401 gtcggcgttt ttagagactt cgcggcgcaa ttcgtgcgcg aatacgttga gcaagacttc
     2461 ggaatcggag ctggtgttga cgtggcgtag gtgtttgttg cacacgtttt cataaagttc
     2521 ggcagtgttg gtgaggttgc cgttgtgcgc caaaacgatg ccgaacggcg agctgacgta
     2581 gaaaggctgc gcctccgcgc tgctgcccgc gttgcccgca gtaggataac ggacgtgggc
     2641 gatgccggcg ttgccgacca aatcgcgcat attgcgtgtg cggaacactt cgcgcaccat
     2701 gcctttgcct ttgtgcatat ggaaggtacc gccttccgcc gttgcaatgc ccgccgcatc
     2761 ctgccccctg tgctgcaaca tctgcaaacc gtcgtacaga agctggttca cgggttcatg
     2821 actgaccaaa cctaatacgc cgcacatatc gtcttctccg attcgaggtt taagggtaaa
     2881 acggaattat aaagcaaacg gtggtttttt gcctgaattg ttgacaatat ttgagtgaag
     2941 gacagatagg tgggtttatg gaggataaga tttaatctgt gatatggctg aggaaaggaa
     3001 aaaaacattt cagacggcat aaaagagaat gccgtctgaa atatccgtat ggcaatcaat
     3061 cgtcttccgg agtttccgcc gtgccgccgc tatggttcaa cacggcttcg gaaagcgata
     3121 cgaaaaacgg cagtgtgtaa gattgccgcc attcttcggt atcgggcagg tcggtttttg
     3181 aagcgagcat gaccagcagg gtaataatca aaatgccttt caatgcaccg aatacgccgc
     3241 ccaaaatgcg gttggcaaag cccaaaccga ccgccgaaac tgccccggtc agcagcgagc
     3301 ggagtatttt ctggatcaga caggcaatga cgaataggga aatgaacgac agagccaatg
     3361 caaacaggcg gggttggaac gaggcaaagg cgaggtcggc gaagggtgcg gcaaagagtt
     3421 tggcaaaaaa gaaggaaacc acccatgccg ccatcgaacc tgcctccgca atcacgccgc
     3481 gcatcgtgga aatgacgatg caggcggcga tgatggcgaa gacgaggagg tcggcaacgg
     3541 ggaggttatt cattcgttac ctgaccggcg ataccgtgta cacgcaattt gttcaaatcg
     3601 cgttcggcat cccttgcgtt tttatagttg cttgatttga cgcggtaaac tttgccgttg
     3661 tcggtcataa tttcggtgat ggtcgaatcg atacccgccg ccttcatttt gcgctggagg
     3721 cttaaggcgc gttctttttc ggcataacct gcctgaatgg cggctttttt accggatttt
     3781 tccttctcgg cggttttggt cttgtccgct ttgtcggttt tttgtgccgt ttcgtgtttt
     3841 ttgccgtccg aacggtcttt ttcggctgtt tttttgcttt cagccttgtc ggcttttttc
     3901 gcttctttta ccgcgctgtc ggattttgcc gtatccggtt tggttttttc ggcggcagtt
     3961 ttcggtttgt cggcaacttt ttcggcggtt ttggtttctt tggctttggg cttggctttg
     4021 gcagtgcgtt ccgctttttg cggttttgtt tcggcagtgc gtttcggttt ttcaaccgct
     4081 accgtatccg tactgtcggc agttgccggc actttttcgg cagcgcgttg ttttgcctgc
     4141 ttcggtgcgg ttttggcggt ttctgcctgt tgcagtttct cggatgcttc caaacctttg
     4201 atgttgctgt cttcgaggcg gtcgttaatc agcaccagcg gcgcgcctac gttttcaggc
     4261 tcgctgattt cgctgtcggc ggcagaaggc ttgtcttcgc ctgccaagtc ctgcggtttg
     4321 tcggcggcgg atttcaaggc aggggtttgt gccgcacctg ccgctttgtt ttctacgccg
     4381 cttgtttcgc cggcagtctg ttcggcaggg ccggaactga gggcggctgc cagcaggatg
     4441 caggaggcgg caaccaggca acttgccgtt acgaggcggc ggcggttgcg ccgtttgagt
     4501 tgttcgtaac cgctcaggac ttcgttttgt ttgttttcgg acatagaagt ttccttttaa
     4561 agtaccgaca tgacatcggc aacggtatga aatgagccga aaacgacgat tctgtcgttc
     4621 tcgcccgctt ttgaggctgc cgcccggtaa gcttcgcgga cggcggcgaa tgtctgtatg
     4681 ttttcgatgc tgtgttcgtg cagtttgttt tgcaggctct cgagcgtcat gccgcgcggt
     4741 acgtccaacg gtgcgatata ccactcgtca aactggtctt taacgatttc caacacgccg
     4801 tctatgtctt tgtcggacag catactgaat acggcggtgc gtttttgcgc aaacgccaaa
     4861 ttaatcaggc tgcggcgcag ggcgcgggca gcgtggggat tgtgtccgac gtccaaaacg
     4921 gtcagcggcc ggccgggcag gacttggaag cgtccgggat tttcaaccag cagcaatccg
     4981 cgtttgattg caccgatgtc caccggcaat ttgtcgttca aacattccaa cacggtcagc
     5041 gcgcaggcgg cgttggaaag ttggtatgcg ccgcgcaatg cggggaaggg cagggaatta
     5101 cggttgcgag cggggctgtc tgaatgctgc ggtcggaagc ggtagttcca ttggatgttt
     5161 tccagcgcgt gaaactcgaa atcgcgctgc accatcagca gtttcgcgcc tatggcttcg
     5221 gcgtgtgaaa gcaacgatgc gggcgcggga ttttgaccgc agattgcggg tttgccgccg
     5281 cggaacacgc ctgctttttc aaagccgacc tgctcgaccg tatcgcccaa aaacgcctga
     5341 tggtcaaaat ctacactagt aaccaccgcg caatcgccgt caaatgcgtt gaccgcgtcc
     5401 aagcgtccgc ccaagccgac ttccaatatc atcacgtcaa cctgttcgcg catgaagatg
     5461 tcgacggccg ccaacgtatt gaattcaaaa taagtcagcg atatttcgcc gcgcgccgct
     5521 tcgatgcgct cgaaagaggc aataatcgta tcgtccgaaa cgggttcggc gttgatggcg
     5581 atgcgttcgt tgtaacgcaa taaatggggg ctggttaatg tgccggtttt gtagcccgcc
     5641 tgtttgtaaa tctgtgtcag gtaggcgcag accgaacctt tgccgttagt tcccgcgaca
     5701 acgacgacgg ggcattgcgg cgtgagcttc atccgttttt tcacttcgcc tacgcgttcc
     5761 aagcccatat cgatcaaacc gccgctgtgg gcggtttcca aatgggagag ccagtcttgc
     5821 agtgttttca ttgagtgttc cgtgttcaaa tgccgtctga aatcagcttg gtgtatcggt
     5881 ttcggcggtt tttttcggct gccgccaaag cacccagact ttcagccgcc ggtaggattc
     5941 cgggtccgcc atatcgtggg tgatgcagtg gcggacggtt ttgccgccgg tgtcccattg
     6001 taagaataag gcataaggcg taaccatact gctgcccgac agtgccgccg cctttgccgt
     6061 tttttctttg ccggatacga tttccgcctg tccgtcgcgg tctatggtaa tggcggttat
     6121 ggcatggcgg tgtttcagat tcgttatcct gagcgagtat gcgtaacttg ccgccaaagc
     6181 cgccaaaccg aaccacatca tccggccgta aaaccaagtc aggcagacgg caagggaagc
     6241 cgagtggagc gatacggtca ggatgttcag gatgcgggac ggcctcaatg ccgtctgaaa
     6301 ggcgcgcaca gccttacatc atgttgtcga acacgggggt aatgttcaat tccgcttctt
     6361 ccatgtccaa caccatatcg tggatttcga tgtcgaaaaa ttcccaaaac gccttcagcc
     6421 ccatatcttg cggccattta tccttatcga tgtcccaacc tgccagctcc gcctcgaaaa
     6481 tctggcggta gcgttcgtcg aaataggaaa cgacggcttc cggctcgtcg aactgcggaa
     6541 cgaggaagac ggagcagttg gcacgaagct gctctatggt caggtcgggc atattttcgt
     6601 cggtgctttt gagccattcc aaaaagcgga cggtcggctt gaggacgacg gcggtgcggt
     6661 caacaaaata catggttttc tttctcaatc atcttgcggt gtcgggatat gctgtctgaa
     6721 cgttcggttt tcagacggca tagcatcaat gggtcatgac ctgttgcagg aactgcttgg
     6781 cgcgttcgtg tttcgggttg gtgaaaaacg cttcgggcgt ttcgtcttcg aggatttgcc
     6841 ctttatcgac gaaaatcacg cggtcggcaa cttcgcgggc aaaccccatt tcgtgggtta
     6901 cgcacatcat cgtcatgccg ctttctgcca agtctttcat cactttcaac acttcgccga
     6961 ccatttcggg gtcgagtgcg gaggtcggtt cgtcaaacaa cattacgcgc ggttccatcg
     7021 ccaaaccgcg tgcaatcgcc acgcgttgct gctggccgcc ggaaagttgg gaagggaagg
     7081 cgtctttttt gtgtgccagt ccgacgcgtt ccaaaagttc catcgctttt ttctctgcct
     7141 gttccgcatt ttgaccttta accttcatcg gtgcgagggt aatgttttcc aacacggtca
     7201 ggtgcgggta gaggttgaaa ctttgaaaca caaagccgac ttcttcgcgg attttgttca
     7261 aatcggtttt tgggtcggca acgttgacac cgtccaccca aatctcgccg ctttcgatgc
     7321 tttcaagctg gttgacggtg cggatgagtg tggatttgcc gctgcccgaa ggtccgcaga
     7381 cgacgaccac ttcgcctttt ttgatttcca agttcacgcc gttgatgacg tgcaggtctt
     7441 tgaaatgttt gtgtacgttt ttgaatttga tcatgtcttt tctttcagac ggcatttgcc
     7501 gttgcaggtt gtcgttacgg gagctccata tgatgaagcg tgtagcgtct gccgtcaaaa
     7561 aaacggtcgt tcggattggt caggcaggct gcaagcggca gtatcagggg taaaagcagg
     7621 tatttcttca tcggcttact cccttttcag acggccttgc ccgccagata attgctcaac
     7681 gctacgtatt tttccggcgc gatatgttcg gcgcggtctt gcggattgat gccgactgcc
     7741 tgcaaatcat cgtcgcctgc aagctctttc agattgttgc gtatggtttt gcggcgttgg
     7801 tggaaggcga gtttcacgag tttggcgaaa tactcgaaat cgtccgccct gccgatgcgg
     7861 tgtttcaccg gaatcatgcg gacgacggcg gaatcgactt tcggcgcggg gtcgaacgat
     7921 tcgggcggca cgtcaatcag catttccata tcgaagaaat attgcagcat cacgcccaag
     7981 cggccgtagt cgttgctttt cggcgcggca accatacgat cgaccacttc tttttgcagc
     8041 ataaagtgca tatcgacgac atcgtccgcc acctccgcca gcttgaacaa aagcggcgtg
     8101 gaaatgttgt agggcaggtt gccgacgatt ttctttttgc ctgcgatgcc gttgaaatca
     8161 aactgcaata catcgccttc gtgaatcacc agtttatccg caaacggcag cgttttcaga
     8221 cggcatacga tgtcgcggtc gatttcgaca acgtgcaggc ggttcagctt tttcgccaaa
     8281 ggttcggtaa tcgccgccaa acccgggccg atttcaatca cgacatcatc cgcctgcggg
     8341 cgcacggcgt tgacaatatc gctgataatc cgcgtgtcct gcaaaaaatt ctgcccgaaa
     8401 cgcttgcggg ctttgtgttc tttcatcgtg tttccttttc ggttgaaacc ccgcccttta
     8461 gggcggtaga atcagactct atttgggagg ggcgtaaccc cttccaaatc aggatggcac
     8521 atagggcggt gctttatgtg tcgtcctgtg tgttgaaaca taaatgtgtt tacagtatcc
     8581 gtttgatgtc ggcattgtaa ccgaaaacgg cagggcgtga taatgctgtt tgaaggcttg
     8641 ccgtgtttgg cggtttggtg caaaaaccgg ctgtctgccg ttttgcctgt tggaggattg
     8701 aacgtgtctg aaaatctgct tgaaatcgaa acccatccct tcgattccgt gttgccgccg
     8761 aaggctgcgg tcatgatgat ggggacgttt ccgcccaagg aagacaaacg cgcgatgcag
     8821 tttcattatc cgaatttcca aaacgatatg tggcgcgttt acgggctggt gtttttcggc
     8881 gatgcggatt atttcaaagt gccgtccaac ggggaggctg cgcactctga aaaagcgttt
     8941 gatgccgaga aaatcaaggc gtttttgcac gaacggggga ttgcgtcctg tccgaccgtt
     9001 ttgaaggcgg tacgtcagca cggcaatgcg tccgacaagt ttttaaaggt agttgaaacc
     9061 gtcgatttgg cggcggtgtt ggcaaaaata cccgagtgcc gccatatttg tacgacaggc
     9121 ggcaaggcga cggaaatcct gctcgatatt cagggtggcg gcatcaaaat gccgaaaacg
     9181 ggcgaaaccg tgctgtttcc gtttgccgga cgggatttga cgctgacgcg cctgccttcg
     9241 acttcgcgca cctatccttt gagtttggcg aaaaaagcgg cggcgtatcg ggcgtttttc
     9301 gaaatggcgg gcttgtgtga aaagcagtta taattgccga caatttcccg ttcggacggc
     9361 atgtttgaaa aaacggaaat gccgtttgaa aacctgaagc acaaggaaga atccgatgaa
     9421 gaactaccac gcgcccgacg agaagggctt tttcggcgaa cacggcgggc tttatgtttc
     9481 cgagaccctg attcccgcct tgcaagagct ggcggatgcc tataaggcag cgaaaaacga
     9541 tcctgaattt tgggcggagt ttcgctgcga tttgaaacat tatgtcggca ggcccagccc
     9601 cgtttaccac gccgcgcggt tgtccgagca tttgggcggc gcgcaaatct ggttgaagcg
     9661 cgaagacttg aaccacaccg gcgcgcacaa agtcaacaac accatcggtc aggcactgtt
     9721 ggcaaaacgc atgggtaaaa aacgcgtcat cgccgaaacc ggcgcgggtc agcacggcgt
     9781 ggcgagtgcc accgttgccg cacgcttcgg tatgacttgc gacgtgtata tgggcgcgga
     9841 cgacatccaa cgccaaatgc ccaacgtgtt ccgtatgaaa ttattgggcg cgaacgtggt
     9901 cggtgtagaa agcggcagcc gcacgctgaa agacgcgatg aacgaagcca tgcgcgaatg
     9961 ggtcgcccgc gtggacgaca cgttctacat catcggtacc gccgccggcc ccgcgccgta
    10021 tcccgaaatg gtgcgcgatt tccaatgcgt gattggcaac gaagctaaag cgcagatgca
    10081 ggaagccatc ggcagacagc ccgacgttgc cgttgcctgc gtgggcggcg gatcgaacgc
    10141 catcggtttg ttccacccgt atatcggcga agaaaacgtg cgcctcgtcg gcgtggaggc
    10201 tggcggtttg ggcgtgaaca cccccgatca cgccgcgccg attacttcgg gcgcaccgat
    10261 tggcgtattg cacggtttcc gcagctatct gatgcaggac gaaaacggtc aggttttggg
    10321 tacgcactct gtttccgcag gcttggatta ccccggcatc ggcccggaac acagccatct
    10381 gcacgacatc aagcgcgtcg aatacactgt tgccaaagac gacgaagcac tcgaagcctt
    10441 tgacttgctc tgccgcttcg agggcatcat ccccgcgctc gaatccagcc acgccgttgc
    10501 ttgggcggtg aaaaacgcgc cgaaaatggg caaagaccaa gtgattctgg tcaacctatc
    10561 cggtcgtggc gacaaagaca tcaataccgt cgcgaagctc aaagggatta aactgtaacc
    10621 tcgtccgtct gatatagtgg attaacaaaa accagtacgg cgttgcctcg ccttgccgta
    10681 ctatctgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactataaaa
    10741 atgccgtctg aagcctgagt tcagacggca ttttattttg ctatgaattt agtattttag
    10801 aaacgaatct gtattttaat ttgtccggat ttttgttttt ccaattgttt tccttttgta
    10861 atactgccat ttacgtttaa tgtaacatta cggtacagta acgcggcacc tgctgaatat
    10921 tgctgttgat tatctgcttt atagacgaag gaattaccgc ccacattcac gccgcctttg
    10981 ccataattgg caaagtaagc tgcagataac aagggtttta cggtaaggtt gccgacttta
    11041 aaccgataag caaaatccag tccggccgtt agtgttttca ctgccataga acttacttta
    11101 acactgtcgt tacccaactt gtaatctgca gatgacaggc ggctgtaacg gattcccgca
    11161 ctggggacaa tctcgaattg attgattttc agcgtattgc ccaaagtaaa gccggtttgg
    11221 atgcttgttc ggttaaagtt tgctttttgc tgcgtttgta accggcttct caagctgcct
    11281 gcaccaatat cgccggccac ataccaagca tcatttaaat aatacttacc ataaaggttg
    11341 gcttgcacaa aagtattttt gccgctcgcc tgatcaaaag tatgctgact gtcagagtaa
    11401 gtcaatacgc cgcctatctg catattttcg gacaagctgc ggtcaatgcc gatttgtgtt
    11461 tgcgtgcgtt tcgaactaaa ccggcgatat tgtgcggaag cataatcacg gccataaccg
    11521 atgttcgaca tccaaacgct gtttttttcg gcatcagcac gtgatttttg tgcaatgtgc
    11581 cgtgttaatg aagcacctgt atccaacaag atagattgcg tgcttgccat tgcgtcagat
    11641 aaagccgagt tggtattggt gctgactgca tcggcttgcg cggcggcttg ggcttgcggc
    11701 tgagcggcgg cttgggcttg cggctgagcg gcggcttggg ctcgcggctg agcggctctt
    11761 ggttgcaaac tcactgtctc aactttttca tgaagttccg tattgtcttg aggctgtttg
    11821 tctgatgtgt caaccgattc ggatacatct tcatcttcca acgcatccaa ggggatttct
    11881 tcataatcat tttcatattt ttcatgaagt tccgtattgt cttgaggctg tttgtctgat
    11941 gtgtcaactg attcagatac attttcatct tccaacgcat ccaaggggat ttcttcatag
    12001 tcattttcat accagtccgg attatgcaag gccctgggtg ctgcgtaagc tgacgaatca
    12061 aatgatggcg agggcggtgc cggcagagta gatctgcgtc cgcgacgttt cggacgattt
    12121 tgggaagctg ccatataatc ctgaggtgcg gcacggcgtt ttcggcgttg cggctgggat
    12181 tgggctgctt gacggtgctc ttcttcctca gcttttcgtt tcgccgattc tgccgcttct
    12241 cgatctgttt cggctttttg ttttgccgac aactctgctg cttggcgttc cgcttcttga
    12301 cgacgtgcaa gttcggcggt ctggcgagct tcttgctggc gttttgctgc ttcggctgct
    12361 gccctttgct tggcaagttc ggcggttttg cgttcggttt ccgctttttg tttgacagct
    12421 aactctgcag cttttcgtgc ttcttcctgt tgacgcgcaa gggctttttg ttggcgtgcc
    12481 gccaatgctt gggcttcagc tgcggctttt tggtttgccg acaattctgc cgctttgcgt
    12541 tctgcttcct ggcgatgtgc aagttcggca gcttggcgtt ttgcctcttc ggcttctgct
    12601 tttcggcgcg cagccaatgc ttgagcctca cgttcggctt ctgccctttg ttttgccaac
    12661 aactctgccg ctttgcgttc ttcctgctgt cggcgcacaa gttctgcttg ggcttgggca
    12721 atttccacat tgttttgctg taccgaacgg tgtccgcggc gtttaggacg gtcttgggaa
    12781 gctgccatat aatcctgcgg tgcggcacgg cgtttgcggc gttgcggctg ggattgggct
    12841 gtttgacggc gttcctcttc cccgaccctt tgttttgccg acaactctgc cgcttcgcgt
    12901 tctttttcat gccggcgtgc aagctctgca gcttggcgtt ttgcctcttc ggcttctgct
    12961 tttcggcgta ccgccaatgc ttgagcctca cgttcggctt cgactttttg ttttgccgac
    13021 aactctgccg cttcgcgttc tttttcatgt cgacgtgcaa gttcggcgct gctgcgttct
    13081 tgttctgctt tttggcgggt cgccagctct cttgcttcgc gttcggcttc tgctttttgt
    13141 tttgctaact ttgccgattt acgctctgct tctgcttgct gacgcttcac ttgctccgct
    13201 tttgcttgct ggcgttttgc ttcttcggct tgatttgcct gcgggctagg cggtgcgacg
    13261 acgatatttt gaggcttggc aatttgtgca ccgtccgttt gtgttgcctt ttgtgcttga
    13321 gaagccgtgt ttgtggcagg agacggggcc ggtttgactc ggcggcggtt ctcggcataa
    13381 ggattgtaca atcgggtaat gccattttct gttttgattg tataacgcaa tgcgcctaaa
    13441 tctacatggt tattcgccaa ggaaacagaa aggcgggagc ggtcttgtac ggatgatgca
    13501 tcaaagagat tcagcccttc ctgattgggt tcgcctgttt tatcttgaac atggagctga
    13561 taatgaccgg atgcggattc cttcacaagc actttatccc caagattttc cgccaagtgc
    13621 gtcagataat gaaaatgccc gttaccggat aaatgattga ttttgagcgt gtggtattga
    13681 tttgcacttt gcgcatcgga ggcattgttc aaatgaatat ggctatccgc caatgacaga
    13741 ttgtgtactt ggctgtcgcc agtcaaatgc catttgctat gttggtttag gctgacacgg
    13801 ctgtttcctt gcccttgaat ttgcccccat aatgcagcct tgcccaagac caatgccgca
    13861 ttctggttca gattcacatt gccgttaatc tgtgtcgctc caaagctgtt taaagcctta
    13921 tcggataagt tgcctgtgtt gcaggtaaca taaccggtat agtccgagcg cacgcaaacc
    13981 tcatcgccgt ttttgtaacc caaatttact ttggcgttgt ctgtcgcggt gatgttggcg
    14041 gtaatgttgg atacatttct accggaagag aatgatgcgg attgattaac cgcaatttct
    14101 gcggctttga atgtgcggtt tatccagtcg tcttcaaata cgacttcatt gtttttggag
    14161 aaatgtgcgt cttttcgggc tgaagatttg ttcacaaaat ctcttgcgtg tggtgttgga
    14221 cgacctgata acaagacatt gccttgagtt acgcttattt ttccgtttaa attagtgccg
    14281 cctgttaaca agaaacggtt ttgcgcgctt ttgccgttga aattaaggtt taatgcaccg
    14341 ttatgtcctt ttccgttttc ttcgccaaag aaaccgctaa aaccgctaat acgctgattg
    14401 tttttgtggt tcatcgcgtt tttcttggct tcttcttgcg tatatcccat aaagacccag
    14461 tcgttatttt ctgttacacc gttctcgggc atcggtgcgt tcacgctgcc gccggatttt
    14521 agggcgtaat aacgatagtt tttgtaataa aggtctttgc cttgcggaat cgggcgcctt
    14581 ggtcggtagt aataactata atcatcttcg tcatagtcat tttgtatgct atgtaccgaa
    14641 aggctgttcg gattggtaat taaagattta cccgttagcg tgattgtgga ggcgtggctt
    14701 gtgttgtggt tgacaatgcg cgcgccttca tccacgttgc ggatgtgttc aaaagtcaag
    14761 tcattgccat tggcatccaa acgaccgcca cggaaaccga aatataggtt atcgggatta
    14821 atctgatttg aactatttaa taccaatgta ccgcgtccgc tgacaatgcc gacttgggag
    14881 aaagcctgga cttttttgtc ggcatcggct tgttgattca gaataaccgt accgtcgccg
    14941 acttttaatt gcccttggtt aacgcctgtg ccgtttattt ctaatgtgcc tttgccgatt
    15001 tttgccaatc tgtcgccatt cggatttttg acttgccaaa cgactttttt gccgtcggta
    15061 acatcaatcc ccgcacctag ccaagtgatg tcattattta tacctttgac tgtgtaatcg
    15121 cctttgaaaa acagaccgcc cgcgccttgg ttgatgtttt gatccaatac caaagtgccg
    15181 ttgtcttcaa aggtaacatt ctgtccgttg tttgcatcct tttcattgcc ggcaagcctt
    15241 accgctgtag aaccaatctt gctgtttgtg ccaaaagtaa tgttccaatg atgttctcca
    15301 ttacctttga tggtgccggc gttatcgcgt tgtttgattt tatctgcaaa ttctttttta
    15361 tagatattcc attcttgcca agagtttttt tgatagcctg cccaataatc gtaagcacct
    15421 aaaaagaccc agcgtttttc ttgtttatca taagcaaata atggtgaacc gctatcgcct
    15481 aacacagcat agttagtcaa tgcgttttga gataaaactt ctttgagttt ttctggggaa
    15541 tgatgttttg aattatcacc gaagccaatc aagccttctt gatttagatt cgatgtgaca
    15601 ttcacgtctt gataaggcgt acctgcaatg gcataacgat aagctcgtga aagctctgtc
    15661 atattgtagc ggctgttata ttcaaattgc gtacctgctc caactcgcac aaactcagaa
    15721 aaacggtttt tatctttata ggtttcaacg ccgccgcctg cactggttgg tgcaataggt
    15781 gcgacttctg ttacgaattt attaaggcgt gccatgttgt agtcttcaag acgaccttga
    15841 ttaccgtgat gccaattttt atttggttcg tagtcatttt gtgcaactga acgatattcg
    15901 ttttcatcat tgctaacatc taagtgccca ttgtgatgcc cgtaataaga gatttcgtct
    15961 ccttttacat gtttgacgct gacggcatac tggggatcga tgacggttaa tgtacgtctg
    16021 ttgacatctg caacgctaaa atcaatcatc ggtacgttgg ataatgcgtt gccgatgttt
    16081 tgaccttgtt tgtttttcac tgataaatcg gttgcgccga caaaaaattt gcctttgttt
    16141 tctgcaaagt cacggaatat ttgataatcg acatcgtctc tgaccaatgc cgcttctgag
    16201 tatggcgtaa gggcataggc aagaaagatg gataaggata tggcgttaat tttaaaacgt
    16261 ttggttttca taaggtttta ccgttttaag ggtgataatg ttttgtattt tacgccggtt
    16321 tggcggtgtt gactattttt tgttgtgtga atcattaatt ttggtgtttt attgccaatt
    16381 taaaaaaaga atcccgatgt ttttatttcc gcttcctttg ttctgttatt caagcgaagg
    16441 cgggaagccg gttttcgggg ttcggttctt ccgttaaatt tctgcggctt tttgtttttg
    16501 gattcccgct tttgcgggaa tgacgggatt ttaggtttct gattttggtt ttctgttttt
    16561 gagggaatga cgggatgtag gttttcttaa ccctgcgtcc tagattcccg ctttcgtggt
    16621 aatgacggga tgtgggttcg tgggaatgac gcggtgcagg tttccgtacg gatggattcg
    16681 tcattcccgc gcaggcggga atccagacct tagaacaaca gcaatattca aagattatct
    16741 gaaagtccga gattctggat tcccactttc gtgggaatga cgaaaagtgg cgggaatgac
    16801 gaaaagtagc gggaatgacg aaaagtggtg ggaatgatgg ttcgggcatt ccttaaatcc
    16861 cccgtgtatc gctgtaaatc ttagagatgg cggaatatag cggattaaca aaaaccagta
    16921 cagcgttgcc tcgacttagc ttaaagagaa cgattctcta aggtgctgaa gcaccaagtg
    16981 aatcggttcc gtactgtttg tattgtctgc agcttcgccg ccttgtcctg atttttgtta
    17041 atccactata aagaccgtcg ggcatctgcg g
//
LOCUS       DAWFLT010000034        16753 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWFLT010000034 DAWFLT010000000
VERSION     DAWFLT010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16753
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.034"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(56..1225)
                     /locus_tag="WGR74_001162"
     CDS             complement(56..1225)
                     /locus_tag="WGR74_001162"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ0905588.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1408..1518)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1571..2467
                     /locus_tag="WGR74_001163"
     CDS             1571..2467
                     /locus_tag="WGR74_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ0905589.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2511..3575)
                     /gene="tsaD"
                     /locus_tag="WGR74_001164"
     CDS             complement(2511..3575)
                     /gene="tsaD"
                     /locus_tag="WGR74_001164"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ0905590.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     GKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVR"
     gene            complement(3691..4878)
                     /gene="ccsB"
                     /locus_tag="WGR74_001165"
     CDS             complement(3691..4878)
                     /gene="ccsB"
                     /locus_tag="WGR74_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ0905591.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4871..6823)
                     /locus_tag="WGR74_001166"
     CDS             complement(4871..6823)
                     /locus_tag="WGR74_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ0905592.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTINRKDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGDGR
                     KRLVADATKGAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7100..7723)
                     /locus_tag="WGR74_001167"
     CDS             complement(7100..7723)
                     /locus_tag="WGR74_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ0905593.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7929..8558
                     /locus_tag="WGR74_001168"
     CDS             7929..8558
                     /locus_tag="WGR74_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ0905594.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPKEN"
     gene            complement(8679..11075)
                     /locus_tag="WGR74_001169"
     CDS             complement(8679..11075)
                     /locus_tag="WGR74_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ0905595.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11227..12342
                     /locus_tag="WGR74_001170"
     CDS             11227..12342
                     /locus_tag="WGR74_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ0905596.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12345..12944
                     /locus_tag="WGR74_001171"
     CDS             12345..12944
                     /locus_tag="WGR74_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ0905597.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            12945..13592
                     /locus_tag="WGR74_001172"
     CDS             12945..13592
                     /locus_tag="WGR74_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ0905598.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13610..14155
                     /locus_tag="WGR74_001173"
     CDS             13610..14155
                     /locus_tag="WGR74_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ0905599.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14174..16507
                     /locus_tag="WGR74_001174"
     CDS             14174..16507
                     /locus_tag="WGR74_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ0905600.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAAAPAKQQ
                     AAAPAKQQAAAPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHT
                     LPTTLQRSLDVADFKTPVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLP
                     KKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTL
                     SLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALY
                     SQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIE
                     KFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNS
                     GFGGDDKWGAETKINLPITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVL
                     TQNRKEAKIESGYEIPFTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKI
                     NKDSPAQCASGNQTILCISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDI
                     PVIGNLFKTRGKKTDRRELLIFITPRIMGTAGNSLRY"
BASE COUNT         4530 a         4763 c         3815 g         3645 t
ORIGIN      
        1 atataaaaat gccgtctgaa ctgttcagac ggcatttttg attttcaaac cggaattaca
       61 gccccgctgc cgccctcaat gcagcagctt tgtcggtgcg ctcccaagtg aactcaggtt
      121 cttcgcggcc gaaatgtccg taagcggcgg atttactgta aatcgggcgc aagagatcga
      181 gcatttggac gatgcctttg gggcgcaggt cgaaatgttc gcgaactaag gtaatcagtt
      241 tttcttcgct gattttgcct gtgccgaaag tatcgatgga aatcgaagtc ggttcggcaa
      301 cgccgatggc gtaggaaact tgaatttggc attgggttgc caagcctgcg gcgacgatgt
      361 ttttcgccac ataacggcag gcgtaggcgg cggaacgatc cactttggac gggtctttgc
      421 cagagaatgc gccgccgccg tgcggagccg cgccgccgta ggtatcgacg atgattttac
      481 ggccggtcaa accgcagtcg ccttgcgggc cgccgatgac gaaacggccg gtcgggttga
      541 tcaggtattt ggtttcgtcg gtcagcagtt cagacggcaa aacgggcttg ataatctgtt
      601 cgatgacggc gtttttcagc tcttcataac cgatttccgg gtcatgttgc gtagagagga
      661 cgactgtatc aatgcgtttt accttaccgg tttcgctgtc gtaaaccacg gtcaactgcg
      721 cttttgcgtc gggacgcagc cacggcaggc ggccgtcttt acgcaattcg ctttggcgtt
      781 gcatcaggcg gtggctgtaa tagatggcaa acggcatcag ggtcggggtt tcgtcgcagg
      841 cataaccgaa catcaaacct tggtcgcctg cgccttggtt caagtcgata ccttcgcctt
      901 cgtttacgcc ttgggcgatg tcgggggatt gttggtcgta gtacacgccg accgcgcagc
      961 cgttggcatc aaagcccagc tcggaggagt tgtagccgat gcgtttgatg gtttcgcgtg
     1021 cgactttgat gtagtccact tgggcggtgg tggtgatttc gcctgccaat acgcacaagc
     1081 ctgtgttgac caaagtttct gcggcgacac gcgctttggg gtcttgcgcc aagatggcat
     1141 ccaaaatcgc atcggatact tggtcggcaa ctttgtccgg atggccttcg gataccgatt
     1201 cggaagtaaa cagatattcg ctcattgaga ttttccttga aaaacaaacc atcttcttca
     1261 gacggcatgt tgtatgaaca taatgtcgac agcgggaaat atagcaaaat ttccctattc
     1321 ataccattca gttgagaaat attcccattt gaatggcact ttggaatctc tgcccgtacg
     1381 tttcttacag gcaaaaaaat tcccgcatca agcgggtttg gattgctctg gtgagccaca
     1441 tcggcttttc aaccgtccac cttacttttc cttttgaaaa gcaggttggc atggaattcc
     1501 caactcttaa tgcagcgcgg atggtagcag aaaagccttt ccgccgcaat acttcccttt
     1561 ttcagacggc atgtttcgtt tacaattcgg gctgtttccc cctttgcgaa ccgccatgca
     1621 catcctgttg accgccctgc tcaaatgcct ctccctgctg ccactttcct gtctgcacac
     1681 gctgggaaac cggctcggac atctggcgtt ttacctttta aaggaagacc gcgcgcgcat
     1741 cgtcgccaat atgcgtcagg caggcatgaa tcccgacccc aaaacagtca aagccgtttt
     1801 tgcggaaacg gcaaaaggcg gtttggaact tgcccccgcg tttttcagaa aaccggaaga
     1861 catagaaaca atgttcaaag cggtacacgg ctgggaacat gtgcagcagg ctttggacaa
     1921 acacgaaggg ctgctattca tcacgccgca catcggcagc tacgatttgg gcggacgcta
     1981 catcagccag cagcttccgt tcccgctgac cgccatgtac aaaccgccga aaatcaaagc
     2041 gatagacaaa atcatgcagg cgggcagggt tcgcggcaaa ggaaaaaccg cgcctaccag
     2101 catacaaggg gtcaaacaaa tcatcaaagc cctgcgttcg ggcgaagcaa ccatcgtcct
     2161 gcccgaccac gtcccctccc ctcaagaagg cggggaaggc gtatgggtgg atttcttcgg
     2221 caaacctgcc tataccatga cgctggcggc aaaattggca cacgtcaaag gcgtgaaaac
     2281 cctgtttttc tgctgcgaac gcctgcctgg cggacaaggt ttcgatttgc acatccgccc
     2341 cgtccaaggg gaattgaacg gcgacaaagc ccatgatgcc gccgtgttca accgcaatgc
     2401 cgaatattgg atacgccgtt ttccgacgca gtatctgttt atgtacaacc gctacaaaat
     2461 gccgtaacga aaataaaaat gccgtctgaa caatttcaga cggcattttg tcatctgacg
     2521 atttccgaca gcggccagcg cggacggaca ttgaacgcac cgacctccct gcccttgcct
     2581 aggtgcatcg caccggcaaa ggcaatcatg gcaccgttgt ccgtgcaata tgccgtcggc
     2641 gggaaaaaca cgctgacttt ttcggacgga tgtttcggct tgcctttggg ggtcgggatt
     2701 tgcaccgtca tattgccgaa agtttcacgg agcttgcggt ttgcaccgac cccgccggcg
     2761 accactacgg ttctgaaccc tgtctgcaac agggcttttt tcactttcgc cgccaacaca
     2821 tcgactaccg catcttgaaa cgcacggcag atgtcgttgc gtgtctgctc aggaatgtca
     2881 tccgccccgt tttccgcgcg cactttctcg acggcggtca atacggcggt tttcaaacct
     2941 gaaaaactca tctgcaaatc gtcggaatga atcatcgggc gcggaaaaac aaacgcttcg
     3001 ggcctgcccg attccgcaag ttccgacagt ttcgcaccgc ccggatacgg caagcccagc
     3061 agtttcgccg ttttgtcgaa tgcctcgccc gccgcatcat cgacgctctc gcccaaaagc
     3121 gcgtagtcgc ctatgcccct gaccgccata atctgcgtat gcccgcccga aaccaacagc
     3181 gcgacaaaag gaaagtcggg tttttcctcc gccaacagcg gcgacagcag atgtccttcc
     3241 aaatgatgga cggggatgac gggcttgccc aatgccaaag ccagtgcatt ggcgtagctc
     3301 gaacccgcca gcagcgcacc gcccaaacca ggcccctgcg taaaggcaac cgcgtcaatg
     3361 tcgccatacg atgcgcctgc ctgcgccaaa cagccttcag tcaacggaac aaggcggcgg
     3421 atatggtcgc ggcttgccaa ttccggcaca accccaccgt attcggcgtg cattgccatt
     3481 tgagtgtgca ggcagtgcgc tcgcaatcct cgttccgtat cgtaaagcgc aacgccggtt
     3541 tcgtcgcaag aagactcgat tcctaatacc aacatggtct gatgccgtta aaaactgaaa
     3601 aacgtatttt agcggatttc ggcacgactg ccgtatccca aaaacggaac atgccgtctg
     3661 aagcccttca gacggcatcg tcgcaccgta tcaaagcgtt ccgtaagaat gcagcccgct
     3721 caaaaacata ttcacgccga taaaagcaaa tgcggttacg aacaaaccga taatcgccca
     3781 ccacgccagc actttgccgc gccaaccggc aaccagccgc aagtgcagcc aaacggcgta
     3841 attgagccag acgatgaacg cccacgtctc tttcggatcc caactccaat agcgtcccca
     3901 agcatctgcc gcccacagcg cacccaaaat ggtggcaatg gtaaagaaca gaaagccgac
     3961 ggcaatcgcc ttatacatca cctcgtcgat caatgccgac ggcggcagcc acagttttcc
     4021 gccttttcct tccgcacgca gggaaaccag ttcggcaata ccgagcatcg cggaaatgca
     4081 aaacgcgccg taaccgataa agtttgccgg aacgtggatt ttcatccacc aagactggag
     4141 cgcgggaatc agcggctgga tggtatgcgc ctcgcgggac acgctgtacc acaagacaaa
     4201 tccaaccacg accgccataa agccgaacac gaagccgccc aatttctgta tggcgaactt
     4261 accttcataa taaagataca tcagcgcggt aatcaccaaa aacaggatga acacttcata
     4321 aaggttggaa accggaatat gccccgcatc gggacggagc agatagcttt cgtgccaacg
     4381 taccagcaga ccggtaaagc ctgctacggc agacacccat gcaaacacgg ttcccatacc
     4441 caacagcgtg ttggtcggca cattttttac gcttgccaaa accgcgcccg aaatataggc
     4501 gaacagggcg aaaaagacaa aggcgcactg ccacataatc gccgactggc tgctgaggaa
     4561 ataccgcaac aggaaaatct ctgccgattt gatgttgccg ccatacagac cgacggcggc
     4621 ataggcaagc aatacgctta aaggaacaaa ccagcgcatc ggtttgaaaa accaacccaa
     4681 aaacacggca ataccggcac tcgcccacaa catgaccgtt tcgtaaatgt ccatatgcat
     4741 accggaacgg gtctgtacga aaaccgtagc cgcaaaaacc agcacggcaa atacccaatc
     4801 ccaaagattc agattgctga tcaaagactt ctgaatcagc agctcgtgtt ccggaagggt
     4861 tttatagtgt tcagtcatga ttcaagtcct tgccgagccg ttgcagactc tcgacgtgtt
     4921 ttggaaattc cttctgcaaa tcccgttcgc tgcgggccga agacatggca aaacggattt
     4981 tgccgtctga aaacaatacc cacgcccgtt tttcgcgcac ataaaacatc aataccgtac
     5041 ccaataccaa cagcaccgag ccgagataga ccaaaagcgc acccggggaa cgggtcatct
     5101 gcaaacccga cgaacgcacc tcggaaaacc cgtcaagctg gagcagcatg ggcgcgggat
     5161 attcggtcaa acccgtgtac gcatccatac tgtgcagcag gaaacggttc cgcgcttcat
     5221 cctgctgcca ttccggcaag ccgtaccggc gtatggtttc atccaaagca gcgttcatca
     5281 cgccgtaaag catttcgtag aaatagccct gcatcttatc ctgctgctct ttcgggatat
     5341 tggacgtaat aaattcgtcc aatcccaaat agcctttttg tgcaaagatg ttcagcgtgt
     5401 tttccgcagc cagcatgaat tgttcgcgga tttcggcagg tgcgcctttg gttgcgtcgg
     5461 caaccagacg tttgcgcccg tccccatctt tcaaaaactc acgcaacgcc ataaaggtgt
     5521 ccgctttcaa ctgcttgtcc aaggggatac gcagccagcg gtattgctgc tgcaagccgc
     5581 tgcgcgtgcc ggtaatccaa aaataatcct gttcctgcaa aaccggcagc atatagtttt
     5641 tatattcgac cgcctgccct gccgcatcac ggatacggta aacaatggaa gggccgatat
     5701 tggtgtattt tttaccttcc tgagtaacgg cgcggacatc gttcagcgtg gatttcaggc
     5761 ttttttcccg ttccgcgccc tcgctcatgt cctccacatt catagaagtg aactgatcga
     5821 actcaagacg atatttgtgt ttgccaattt ccaacggaaa ctggtgtatg gatgttgcct
     5881 tcaacacgac aggctcgcgc gaagcatcac ccaaattcca cgccttgaat gtcaaatccg
     5941 aaccgccgtc ggcaaaactc gcctgataaa tcgtgatgcc gtgcaaggtc aaaggatggt
     6001 tcacgcggat ggtgcgctcg agtttctcac cggttgcctt gtccgtcact tcaatatcgc
     6061 tggcgaaatc gcgcggcata ccggtattgt aaaaatcgat atggaatttt ttcagtttga
     6121 cttcaaaagg caagtcctga accaatatcc cgttgtcggc attcaggaaa accacatccg
     6181 cactctgccc ctcggaaata ttgacgttgc ccctaaatga gagattggac gcacccaaaa
     6241 tactttcggg cttgaaatcc ttggcataaa ccgcctgatt gtccggaaca atccgaccgg
     6301 tcagcatacc cagtttcaac agcaggttac tgtctatcaa cccgcccagg caaatgacaa
     6361 tcaaagcaac atgggcaaag atatagcccc atttgttcat tgtgcctttt ttggcggcaa
     6421 tcagaaccga cccgtcttta cggttaatgg tttttccctg aaaaccttgt acttccagat
     6481 aacgtttggc aacctcgggc gcaattttta catccaacag cgaagaatgg cgcatcgccg
     6541 ccagagattt ttctttaacc ttttcccgaa aagacttcat ttcgcgccag aacggcggca
     6601 cattgcgaat caggcacaaa ctggtagaaa ccaccaaaaa catcatgata acgacaaacc
     6661 atgccgaagc atagacgtca tacagtccca gaaaaccaaa aatctgcgcc caaaaaggtc
     6721 cgaatttgac caaataatcc gtctgcggct ggttttgctg caacaccgta ccgataaccg
     6781 atgcaatacc cagcagactg agcaaagcga ccgcaaagcg catggagctg aaaaaagcga
     6841 accacggacg ggaaagaagt gggggagatc tacgggattt actcattgtg tgtttattcc
     6901 gccatcagga atatgggaaa gcagaattgg gcaaacagaa aacaacgtcc cgattctact
     6961 gtcttgatgc tttttatttc aagacaatga agacagcctg catcgattcc aacggttgcg
     7021 attgaaaaaa cttatcgcag aattgcctga agccgtctga aaacttttca gacggcctct
     7081 aaaacagact attgcggaat taacgcaaac cttggataaa gttggcgacc gctttcaaat
     7141 cttcttcaga catacggttt gcaatatctt ccatgatggt atttttacgc tgaccggact
     7201 tgtaggcatt catctgttca acaatatatg cctgatgctg accgcccaaa cgcggataag
     7261 cctgaatttc gcttccgcct cccggcatac ccgcaccgct cggaccgtgg caggacatac
     7321 acgccggcac ttttttatcg ctcaaaccgc cgcgatagat tttcgcaccc aattcgggat
     7381 tttccttagg attggcttca ccggatttgg gctgctgttt ggcatagaat gcggatacgt
     7441 tcaaaatatc ctgatcgctc aaattcatta ccaccggttt catcacagct gccgaaccgt
     7501 gggtgcgttt accgtcgcgg atgccgatgg tttgatgata gatgtaagca gtatgctgtg
     7561 ccgccaaacg cggatacatc gcaatgccgc tgttaccgtc tgctgcatgg caagccgcac
     7621 aaaccgttgc ggcaacctgt ttgccttttt ccacgtctgc tttgggagat gcggaaaccg
     7681 caccggcagc caaaacaaag gccaataaag tcaatcgttt catggagtgc tcctgattac
     7741 agcattggat aacgcaacaa tgctcttttt atattcaaat acgggatttt tgacccgatt
     7801 aaaaccgatg attctgcaaa cgtgttattc tatactaaat ttacattaaa ttaccactgt
     7861 gtttcacata aaaccaaccg catatttttg ctgtcggaca aacggcggcg gaaaacaagg
     7921 atatgcccat gaaccttttt caaaacgcca aattcttcac gacggtaaac catctcaaag
     7981 acctgccgga cacaccgctc gaaattgcct ttgtcggcag gagcaatgcc ggaaaatcca
     8041 gtgccatcaa taccctgacc aaccatgtcc gtcttgccta cgtttcaaaa acacccggac
     8101 ggacgcagca tatcaacttc ttcgagctgc agaacggcaa ttttatggtc gatttgcccg
     8161 gctacggtta tgcccaagtc cccgaagcag tacgcgcaca ttgggtcaat ctgctcggcg
     8221 actatctgca acagcgcaaa cagcttatcg ggctggtttt gattatggat gcccgccatc
     8281 ctttaaaaga actcgacatc cgtatgctgg actttttcca cacgaccggc agaccggttc
     8341 acatcctgct gtcaaaagcc gacaaattat ccaaaaacga acagataaaa accctgtccc
     8401 aagtcaaaaa actgctcaaa ccttattccg acaggcaaaa catcagcgta cagctgtttt
     8461 ccagcctgaa aaaacaaggt attgacgagg ccaaccgaac tgtcggaagc tggtttgacg
     8521 cagcagatgc cgccgcttcc tctccaaagg aaaactgacc ccaattatac ggaaaccgta
     8581 ttccccccac ttgaccgacc gcaaacattt aaaaaattgc cactgccaaa tctaaaatgc
     8641 cgtctgaaag acttttcaga cggcattttg cggagtcttt aaaacagaga atccaactgc
     8701 tgctgtttgg aaccagtatt actcggaagc accggcgttt cctgcatatc ttggcggact
     8761 tcgtcatccg ccgcctgccg tccgccttct gccgcgcccc cgtcatcttc ttttgcccgt
     8821 cgggaaggtt gcggcgcaat accgctgttg tccagcgtca agcccggatc ggttaccata
     8881 cgttccttca tatagtattc gccattgctg ctgaccacac cttcaggcat tttcatcccc
     8941 ttgccctgct ttcctttcaa cgcaaaacgc atatagtcca cccaaaccgg caccgcaatc
     9001 gtaccgccgt agccgacacg ccccatactc ttaggtttgt cgaagccgat atatacggca
     9061 gtaaccacat cagggttaaa accgacaaac cacgcatcct tattgtcatt ggtcgtaccc
     9121 gttttaccgg caatatccgt tcttcccaac gcagctgccc cccttgccgt accaacacgg
     9181 accacatcct gcataatctt atacataata taggcattgc gcggatcgat tgcctgaggc
     9241 gcattttgcc cggccaccaa aggttgcatt tgggcgcgca acctgccgtc tctgtcataa
     9301 atcttatcga ttacgtgcga agaaacccta tatccgccgt tcgcaaatac gctatatgcc
     9361 tccgccactt tcaacggcgt tgtctcgccc gtacctaaag ccatagacag gcttgccggc
     9421 agctcggacg acctgaagcc gaaacgccgg atatactgtt gcgcgtaacc gacaccgata
     9481 gacatcaaaa tacggatgga aaccatattc ttggaagccg tcagagcctg tctcaaagta
     9541 atgtagccgg aatatctgcc gtctgaattt ttaggtgtcc aaaccgaacc gttcggccct
     9601 ttccccggca gggaaatcgg cgcatcgtta accactgtgg acgcggtcat ccccttagat
     9661 aatgccgccg aatagacaaa cggcttaaag gtcgaacccg gctgccgcat tgcctgaacg
     9721 gcacgattga atgttttgct gtgaaaatca taaccgccga ccagcgcgcg cacagctccg
     9781 gtttttgcat ccagcgaaac caaagccccc tgcagcaacg gctcttgaac caccgcccaa
     9841 cgcccgccgt tgtttttgac acggatgacc gcgcccctgc ggatacggtc ctcccccatt
     9901 ttttcattat tgaccgcgcg ggccgcaaaa cccaaggcgc gcctgtcaag cgtaacccgc
     9961 ctgccgccgg gcagctgtat gacgacattt ttctttttag tcacatccaa cacaacggcg
    10021 ggaaccattt tatcgacggt atagagtccc gacagatact ggctgacagt ctcctcgaca
    10081 tcttcactct tactcaaatc gatatagttt tccgcaccgc ggtagctgct gccgcgatcg
    10141 aaattccgta gagccttgcg caatgcctcg gttgccacct tctgatgatc ggcgcggacc
    10201 gtggtataaa ccttaaaacc ctgcgtatag gcatcttcac cgtatttctc atacagttcc
    10261 tgacgcacca tttccgccac atataaagca ctctgatcga ttttccgaac aaaccgctcg
    10321 taatgcagtt cctcattcaa cgcctgatcg cgctgttgca cggtaatcat cttctcctcg
    10381 agcatattgt tcaaaatata cttctggcgc aacttggcac gttctggatt aacaatcgga
    10441 ttataggcag acggagcctt gggcagtccc gcaagcatgg cggcttccgc caaagtcaaa
    10501 tctcggacat tcttattgaa atagatttgc gcggcagatg caaaaccata ggcgcgctga
    10561 ccgaggtaaa tctgattgaa atacaactcg aggattttgt ctttgcttaa agactgctcg
    10621 attttatagg caagcaacac ctcattgaat ttgcgtgtga acgttttttc actgctcaaa
    10681 taaaaatttt tcgccacctg ctgcgtaatc gtactcgcac ccgactgcac gctgccggac
    10741 acgacattgc cgacggcagc gcgggcaaca ccccaaacat ccacccccca atgccggtaa
    10801 aagcgtttat cctcggcggc gataaccgca ttccgcaaca cctctgggaa atcgccgatt
    10861 tttgtaaatt cgcgccgctg ctccccatac ataccgatga cttccccatc cgccgaataa
    10921 atagtcaacg gcattttagg ctggtaatgc tgcaaagaat ccaaagacgg cagtttcgga
    10981 tacgttacca aaatagcaat ggcaaccaaa cccactccaa atacacaaaa cccaaaaacc
    11041 aaaccaaaac aagtcgttaa aatcttttta atcatagctg aataataatt taccattatt
    11101 ggtattaaat aaagtaaaat agcaaccgat ttctacaaag cacggtttca atgtgcaaag
    11161 aacaaggaat ccattacgga taccgaaacg gttactcact gtacaaataa agcaggaact
    11221 ttcatcatgc gcttgtttaa aagcttgaaa aaccctaaaa aaacagatgc caagctccct
    11281 aaaaaatctt cgggactcaa taaccgcgcg gcaatcggca tcgatatcga ccagcattcc
    11341 atcaaaatgg tccaattgtc aggacgtagt ttaaaccaaa ttcaattgga aaaatacgtc
    11401 attgccaaat taccaaagaa tatcattcaa ggcaataaag tccaaaatta cgatcaactt
    11461 gttacatatt tgcaacaagc ctatgccaaa ctgggtactt cgtgcaaaaa catcatcgcg
    11521 tccgtcccgc aaaatctggc aaccatcgaa caattgacct acacagacaa agatgcagaa
    11581 ttagacctgc aggggttcgt ggagtcctcc atctccgaag tcagctcgat atcgctcgaa
    11641 gaagccaatt acgactatca ggtcttgtcc caatcggccg ccggcgaagc tgtgttggcc
    11701 gtcgcatcga gaaaggatga aatcgaaccc ctgattgacg cattcaacgc cgccggtatg
    11761 aaattatccg cgcttgatgt ggacattttc ggacaataca acgcctacgc gctatggata
    11821 aaccatttcg cccccgagct tgcagccgaa aaagtcgcca ttttcggcgt atatgccgca
    11881 cagacctacg ccttggtcat ccaagacgga aaaatcctat acaaacagga aacctccgtc
    11941 agcgaagaac agctcaacca actcatccag cgcacctatc aggtaacaga agaaaaagcg
    12001 gaagaaatca tcaactcccc gcaaaaacct tccgattacc aagaaagcgt ggcaaactat
    12061 ttcaaccagc agattaccca agaaatacaa agggtcttgc agttttatta caccacgcag
    12121 accgcagacg atatgaccga catcaagcat atcctgctga ccggggaagc ggcgcgccag
    12181 gaaggcatcg cccaaaccgt cgcctcacaa accaatgcag atgtacaatg cgtccatccc
    12241 gcgcgttatt ttgcggacaa cctcaaaaca gacaaacaac aattcgaact tgatgcgccg
    12301 acactgacca gggcgttcgg tttggcggta cggggattat aattatgaac aatttaatca
    12361 aaatcaacct cctcccctac agggaagaga tgaacaagcg caaacagcag cagtttaaaa
    12421 cgctgatgta cggtgccgtg ctgacgggcg ttgccgccgt tgcggcaacc tacctgttta
    12481 tcgacaatat gatcaataac cagtcggaaa gaaacacgct gctggaaacc tccatcgcac
    12541 acttggatac cgagctgtcg gaaatacaaa agctcaaaca ggaaaaagat gccttcctga
    12601 ttaagaaaaa caaaatcgag gagctccagc tcaaacgcct ccaagccgca aaaatcctcg
    12661 acagcctgaa tgaggccgtc cccggaagca cctacctgac ctcgctggat gccgttaccg
    12721 ccgactctta tcggctcagc ggcaggacat ccagcgacaa ccgcgttgcc gccatgatga
    12781 gggcgatgcc caataccggc atattcaagc aacccgaatt gttaagcatc aagaaaaaca
    12841 attcgcatca agaatttacc cttcaggcaa cattacaacc catcgtaaag gcggccgaat
    12901 ccaaagagaa tccggcttcg ggaaacgcac aggaggcaaa ctgaatggct tctaaatcat
    12961 ctaaaaccaa cttggatctc aacaaccttc acctgctcaa ccttcctgcc aggcttttta
    13021 tcgccctgct ggccgttgcc gccgtgctgg ggctcggtta tgccggattg ttcaaaagcc
    13081 agatggaatc ccttgaggaa tacgaagcaa aagaaaccga actgaaaaac acctacaaac
    13141 agaaaagtat cgacgcggcc agcctgaaca acctgaggga cgaacttgcc tcaatccgct
    13201 ctgccttcga tatcatgttg aaacagctgc cgacagatgc agaaattccc aacctggttc
    13261 aagagcttca tcaggcaggt tcgagcaacg gcctgcgctt ggacagcgtt atgccccaac
    13321 ctcccgtaga tgacggcccc atcaaaagat taccctattc catttccatt accggaaatt
    13381 acgaacagat cagccaattt acccgcgatg tcggcagcct ctcccgaatc attacccttg
    13441 agtcgctgaa aatcgcccaa tctccggaaa acggcggcaa tcctgacggc aagagcagca
    13501 tcctgaacct cagtgccatt gccaccacct accaagcaaa atccgtagaa gagcttgccg
    13561 cagaagcggc acaaaatgcc gagcaaaaat aacttacgtt agggaaacca tgaaacacta
    13621 tgccttactc atcagctttc tggctctctc cgcgtgttcc caaggttctg aggacctaaa
    13681 cgaatggatg gcacaaacgc gacgcgaagc caaagcagaa atcatacctt tccaagcacc
    13741 taccctgccg gttgcgccgg tatacagccc gccgcagctt acagggccga acgcattcga
    13801 cttccgccgc atggaaaccg acaaaaaagg ggaaaatgcc cccgacacca agcgtattaa
    13861 agaaacgctg gaaaaattca gtttggaaaa tatgcgttat gtcggcattt tgaagtctgg
    13921 acagaaagtc tccggcttca tcgaggctga aggttatgtc tacactgtcg gtgtcggcaa
    13981 ctatttggga caaaactacg gtagaatcga aagcattacc gacgacagca tcgtcctgaa
    14041 cgagctgata gaagacagca cgggcaactg ggtttcccgt aaagcagaac tgctgttgaa
    14101 ttcttccgac aaaaacaccg aacaagcggc agcacctgcc gcagaacaaa attaagaaga
    14161 ggattactcc attatgaata ccaaactgac aaaaatcatt tccggtctct ttgtcgcaac
    14221 cgccgccttt cagacagcat cggcaggaaa cattacagac atcaaagttt cctccctgcc
    14281 caacaaacag aaaatcgtca aagtcagctt tgacaaagag attgtcaacc cgaccggctt
    14341 cgtaacctcc tcaccggccc gcatcgcctt ggactttgaa caaaccggca tttccatgga
    14401 tcaacaggta ctcgaatatg ccgatcctct gttgagcaaa atcagtgccg cacaaaacag
    14461 cagccgtgcg cgtctggttc tgaatctgaa caaaccgggc caatacaata ccgaagtacg
    14521 cgggaacaaa gtttggatat tcattaacga atcggacgat accgtgtccg cccccgcacg
    14581 ccccgccgta aaagccgcgc ctgccgcacc ggcaaaacaa caggctgccg caccgtctac
    14641 caagtccgca gtatccgtat ccgaaccctt taccccggca aaacaacagg ctgccgcacc
    14701 gtttaccgag tccgtagtat ccgtatccgc accgttcagc ccggcaaaac aacaggcggc
    14761 ggcatcagca aaacaacaga cggcagcacc agcaaaacaa caggcggcag caccagcaaa
    14821 acaacaggcg gcagcaccag caaaacaaca ggcggcagca ccagcaaaac aaaccaatat
    14881 cgatttccgc aaagacggca aaaatgccgg cattatcgaa ttggcggcat tgggctttgc
    14941 cgggcagccc gacatcagcc aacagcacga ccacatcatc gttacgctga aaaaccatac
    15001 cctgccgacc acgctccaac gcagtttgga tgtggcagac tttaaaacac cggttcaaaa
    15061 ggttacgctg aaacgcctca ataacgacac ccagctgatt atcacaacag ccggcaactg
    15121 ggaactcgtc aacaaatccg ccgcgcccgg atactttacc ttccaagtcc tgccgaaaaa
    15181 acaaaacctc gagtcaggcg gcgtgaacaa tgcgcccaaa actttcacag gccggaaaat
    15241 ctcccttgac ttccaagatg tcgaaatccg caccatcctg cagattttgg caaaagaatc
    15301 cgggatgaac attgttgcca gcgactccgt caacggcaaa atgaccctct ccctcaaaga
    15361 cgtaccttgg gatcaggctt tggatttggt tatgcaggca cgcaacctcg atatgcgcca
    15421 acaagggaac atcgtcaaca tcgcgccccg cgacgagctg cttgccaaag acaaagcctt
    15481 cttacaggcg gaaaaagaca ttgccgatct gggcgcgctg tattcacaaa acttccaatt
    15541 gaaatacaaa aatgtggaag aattccgcag catcctgcgt ttggacaatg ccgacacaac
    15601 cggaaaccgc aatacgcttg tcagcggcag gggcagcgtg ctgatcgatc ccgccaccaa
    15661 taccctgatt gttaccgata cccgcagcgt catcgaaaaa ttccgcaaac tgattgacga
    15721 attggacgta cccgcgcaac aagtgatgat tgaggcgcgt atcgtcgaag cggcagacgg
    15781 cttctcgcgc gatttgggcg ttaaattcgg cgcgacaggc aagaaaaagc tgaaaaatga
    15841 tacaagcgca ttcggctggg gggtaaactc cggcttcggc ggcgacgata aatggggggc
    15901 cgaaaccaaa atcaacctgc cgattaccgc tgccgcaaac agcatttcgc tggtgcgcgc
    15961 gatttcctcc ggtgccttga atttggaatt gtccgcatcc gaatcgcttt caaaaaccaa
    16021 aacgcttgcc aatccgcgcg tgctgaccca aaaccgcaaa gaggccaaaa tcgaatccgg
    16081 ttacgaaatt cctttcaccg taacctcaat cgcgaacggc ggcagcagca cgaacacgga
    16141 actcaaaaaa gccgtcttgg ggctgaccgt tacgccgaac atcacgcccg acggccaaat
    16201 cattatgacc gtcaaaatca acaaggactc gcctgcgcaa tgtgcctccg gtaatcagac
    16261 gatcctgtgt atttcgacca aaaacctgaa tacgcaggct atggttgaaa acggcggcac
    16321 attgattgtc ggcggtattt atgaagaaga caacggcaat acgctgacca aagtccccct
    16381 gttgggcgac atccccgtta tcggcaacct ctttaaaaca cgcgggaaaa aaaccgaccg
    16441 ccgcgaactg ctgattttca ttaccccgag gattatgggt acggccggca acagcctgcg
    16501 ctattgatgc gtcaaaataa gggcatatgt tttacggcat atgccctttc tttatgcttt
    16561 ttgccgcgac cgaaatgccg tcattcccgc gcaggcggga atccaacttg tccggttccg
    16621 gttgtttttc gtctcgtaac ttttgagccg tcattcccgc gcaggcagaa atccagtccg
    16681 ttcagtttcg gtcatttccg ataaattcct gttgcttttc atttctggat tcccactttt
    16741 gtgggaatga cgg
//
LOCUS       DAWFLT010000035        16615 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWFLT010000035 DAWFLT010000000
VERSION     DAWFLT010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16615
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.035"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(446..3235)
                     /gene="ileS"
                     /locus_tag="WGR74_001175"
     CDS             complement(446..3235)
                     /gene="ileS"
                     /locus_tag="WGR74_001175"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ0905601.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQVARQKKDFIRLGVLGDWDKPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVGYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGDHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMSVWEANPVILQWLEEIGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKGELLSAEDCEHYDKLSDTMDVWFDSGSTHYSVVKQREELDWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYAVVLARRLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
     gene            complement(3376..4296)
                     /gene="ribF"
                     /locus_tag="WGR74_001176"
     CDS             complement(3376..4296)
                     /gene="ribF"
                     /locus_tag="WGR74_001176"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ0905602.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVAVIFEPQPKEFFAALAGKTPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMAELKRQIEADMEAAKCW"
     gene            complement(4823..6118)
                     /gene="tyrS"
                     /locus_tag="WGR74_001177"
     CDS             complement(4823..6118)
                     /gene="tyrS"
                     /locus_tag="WGR74_001177"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ0905603.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKISLYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     SQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLYQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAVEAKDQASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(6181..7968)
                     /locus_tag="WGR74_001178"
     CDS             complement(6181..7968)
                     /locus_tag="WGR74_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0905604.1"
                     /translation="MAAGRISRCRYILCDFRLFDDGDPPSRNVGGGRFSLKAFYIRRI
                     KRILPAFFAVLAATLAGGFFLFTKDDFFLLWKSALTALGFASNLYFARGKDYFDPAQE
                     EKPLLHIWSLSVEEQFYFVFPILLLLVARKSPRVQFGFLAALCALSLAASFMPSAFDK
                     YYLPHLRACEMLVGSLTAVWMRYRQQRNLAVGRCCAAAGALFSACILSACLFAYSEQT
                     AYFPGPAALIPCLAVAALIYFNHYEHPLKKFFQWKITVAAGLISYSLYLWHWPILAFM
                     RYIGPDNLPPYSPAAAVVLILLLSLFSYHCIEKPFKKWQGSFAQSVLWIYALPMLILG
                     AGSFFAMRLPFMAQYDRLGLTRSNTSCHNNTGKQCLWGDTEKQPELLVLGDSHADHYK
                     TFFDAVGKKEKWAATMVSADACAYVEGYASRVFQNWAACRAVYRYAEEHLPRYSKVVL
                     AMRWGSQMPENSRSLAYDAGFFKKFDRMLHKLSSEKQAVYLMADNLASSYNVQRAYIL
                     SSRIPGYRQTLRPDDESTLKANARIRELAAKYPNVYIIDAAAYIPANFQIGGLPVYSD
                     KDHINPYGGTELAKRFSEKQRFLDTRHNH"
     gene            complement(7860..8087)
                     /locus_tag="WGR74_001179"
     CDS             complement(7860..8087)
                     /locus_tag="WGR74_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0905605.1"
                     /translation="MRRRDESKNIMSQALPYRPDIDTLRAAAVLSVIVFHIEKNWLPG
                     GFLGVDIFFVISGFLMTAILLREMSGGGGFP"
     gene            complement(8327..9418)
                     /gene="ychF"
                     /locus_tag="WGR74_001180"
     CDS             complement(8327..9418)
                     /gene="ychF"
                     /locus_tag="WGR74_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ0905606.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEELAMLKPLFLLTAKPAMYVGNVSEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEEDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(9532..11208)
                     /locus_tag="WGR74_001181"
     CDS             complement(9532..11208)
                     /locus_tag="WGR74_001181"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ0905607.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNVDNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDSVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLADLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLAHKVVNAIESQTNNFGFAYDVELGIKDKI
                     RVIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            11383..11814
                     /locus_tag="WGR74_001182"
     CDS             11383..11814
                     /locus_tag="WGR74_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0905608.1"
                     /translation="MSSNAWLFWALASAGFASLTAIFAKMGLQGIDSDFATFIRTLVI
                     LAALLLFLTYTGKWQGVNGFTGHNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            complement(12045..12740)
                     /locus_tag="WGR74_001183"
     CDS             complement(12045..12740)
                     /locus_tag="WGR74_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ0905609.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQTASSLPEHISAHLWLVENPPHNPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(12756..13256)
                     /gene="hpaC"
                     /locus_tag="WGR74_001184"
     CDS             complement(12756..13256)
                     /gene="hpaC"
                     /locus_tag="WGR74_001184"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ0905610.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRKARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALVHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            complement(13371..13811)
                     /gene="nadR"
                     /locus_tag="WGR74_001185"
     CDS             complement(13371..13811)
                     /gene="nadR"
                     /locus_tag="WGR74_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ0905611.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTCLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(14154..14663)
                     /locus_tag="WGR74_001186"
     CDS             complement(14154..14663)
                     /locus_tag="WGR74_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ0905612.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWVRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDRTVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSSA
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            complement(14742..14817)
                     /locus_tag="WGR74_001187"
     tRNA            complement(14742..14817)
                     /locus_tag="WGR74_001187"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14782..14784),aa:Lys,seq:ttt)
     gene            15028..16107
                     /gene="apbC"
                     /locus_tag="WGR74_001188"
     CDS             15028..16107
                     /gene="apbC"
                     /locus_tag="WGR74_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ0905613.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIAAAVADSVQEILMPETGGAHIHLSIDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGATAQLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
BASE COUNT         3805 a         4534 c         4472 g         3804 t
ORIGIN      
        1 cactatagat tcccacttcc gtgggaatga cggttcagtt gcattccgac aacaccgtaa
       61 tcttgaaatc cgtcattccc gcgcaggcgg gaatccagac ccctgacgcg gcgggaatct
      121 atcggaaatg actgaaaccc cgagattcta gattcccact ttcgtgggaa tgacggttca
      181 gttgcgttcc gacaacaccg caatctcgaa atccgtcatt cccgcacagg cgggaatcca
      241 gacccacgac gcggcgggaa tctatcggaa atgactgaaa cctcgagatt ctagattccc
      301 actttcgtgg gaatgacggt tcagttgcat tccgacaaca ccgcaatctt gaaacccctc
      361 cgccgttata aagacaaatc acggcacaaa aaatgccgtc tgaaatgctg ttcggcggtt
      421 tcagacggca tttgctcaaa ctttatcagg cgtaatggcg cgtttcgcct tctccgccga
      481 cattctctgc acagcgtttg cagacggttt catagcctgc aaccgcgccc acatcgcggg
      541 tgtagtgcca gcagcgttcg catttttcac catcactggc tttagcggca acggcaagtt
      601 cgctgcctac tttcacttct gctttagaca ccagcaaagc aaagcgcaat tcttcgccca
      661 aagcattcag atagccggcc atttcttccg gcgcggtaat ttcggcttcg gcttgcaagg
      721 acgaaccgac ggttttgtcg gcgcgcaaag gctcgatggc ggcggttacc gcttcgcggg
      781 cttcgcggat tgccgtccat tttttcacca gttcggcttc ggctttttcg ttgatggtcg
      841 ggaactcgtg ccaggtgtgg aagaggacgc tgtcttcttc gccgccgccg atgatgtccc
      901 acgcttcttc gccggtaaag cacaaaatcg gcgcaatcaa gagaaccaaa ctgcgcgtga
      961 tgtggtacag ggcggtttgc gcgctgcggc gggcgcggct gtcggctttg gtggtgtaga
     1021 ggcggtcttt caggatatcg aggtagaacg cgcccaagtc ttccgagcag aaagaaacaa
     1081 tgtctttcac ggcaaaatgg aaggcataac gcggatagta atcgcctgcc agacgctctt
     1141 gcagccgacg cgccaatacc acggcgtagc ggtcgatttc caccatgtct gcctgttgca
     1201 cggcatcttc aataggattg aagtcgctga ggttggcaaa caggaagctc aaggtgttgc
     1261 ggatacggcg gtagctttcg gttacgcgct tgaggatttc tttggaaatc gccaattcgc
     1321 cgctgtaatc ggtggatgcc gcccacaggc gcaggatgtc cgcgccgaac tcgttataca
     1381 cttcttgcgg cgcgacgacg ttgccgatgg atttcgacat tttgcgcccg ttttgatcca
     1441 ccacgaaacc atgggtcagc agctgtttgt acggcgcgcg acccattgat gaggcgcagc
     1501 cggtcagcat ggacgactga aaccagcccc ggtgttgatc gctgccttcg aggtacaagt
     1561 cagcaggcca gtccaattct tcgcgctgtt tcacaacgga ataatgggtc gaaccggagt
     1621 cgaaccatac gtccatcgtg tcggagagtt tgtcgtaatg ttcgcaatct tccgcgctca
     1681 acaattcgcc cttatcgagg gagaaccaag cctcgatgcc tttttcttcg atgcgttgcg
     1741 cgactttttc caaaagttcg gcagagttcg gatgcagctc gcccgtttct ttgtgaacaa
     1801 agaaagtcat cggcgtgccc caatagcgtt ggcgcgaaac cacccagtcg gggcggcctt
     1861 caatcatggc ttccaaacgc gcacgacccc atgatgggaa aaattcagtg tcatcaacgg
     1921 ctttgatggc tttatcgcgc agggttttac catcggcacc tgctttatcc ataccgacaa
     1981 accattggcc ggtcgcacgg taaatcaacg gcgttttgtg acgccagcag tgcgcgtagc
     2041 tgtgttcgat tttgctgctt gccaagaggt tgccgatttc ttccaaccat tgcaggataa
     2101 cgggattggc ctcccaaacg ctcatgccgg caacgcgcgg cgtttcactg atgtatttgc
     2161 cttcggcgtt gacggggttg taaagctcga tgccgtattt attgcagacg gcgtagtctt
     2221 ccaaaccatg agcaggcgca gtatgcacca aaccggtacc ggcgtcggtg gtaacgtggt
     2281 cgccgttgag catgggaata tcgcgttcga ggaacggatg attcatgtgc aggttttcca
     2341 gcttgtcgcc ggtggtttcg gcaagaatag caatgccgtc tgaaaaaccg taacgtttga
     2401 gcgcgtcttc tgccaaatct ttcgccagca ccaatttgcc tttgggcgta tcgattaatt
     2461 gatacaccac gtccgcaccg gcagacacgg cttggctcgc tggcagcgtc caaggcgtgg
     2521 tcgtccagat gacggcaaac gctttgccct cgatgcctgc caaaccgaat gcagcggcaa
     2581 gcgcagcggt gtctttaaac ggatagccca cgtcaatcgc aggcgatact ttgtctttgt
     2641 attccacttc ggcttcggcc aatgaagagc cgcagtccaa gcagaactga accggtttcg
     2701 cgccgcggta gagatagccg gatttgtaga tttcgccgag catacgcacg gtatcggctt
     2761 cggttttaaa atccatggtc aggtagggct tgtcccagtc gcccaacacg cccagacgga
     2821 taaagtcttt tttctggcgg gcaacttgtt cggcggcata ttcgcggcac aattcgcgga
     2881 aacgcgcttt gggcatgtct ttgccgtgca gcttttctac catcacttcg ataggcaaac
     2941 cgtgacagtc ccaacccggc acataaggcg cgtcaaaacc ggcttgggtt ttgctgcgga
     3001 taatgatgtc tttgagaatt ttgttgacgg cgtgaccgat gtggatgtcg ccgttggcat
     3061 acggcgggcc gtcgtgcagg atgaacttcg gacgaccttt ggcgatttcg cgcagttttt
     3121 ggtagcgttt ttgctcgtac cagcttttca gccatgcagg ctcgcgcttg gcaagattgc
     3181 cgcgcatcgg aaacgggctt tcgagcaggt ttacggtttt gctgtaatcg gtcatttttt
     3241 aatctctatt gttacaatat ttcggttcag acggcattgc gcctcaaaca gtatttcaca
     3301 acgggaaacc cctatgccgt ctgaacagtt aaaaggttga ttgtagccca atcggatggt
     3361 ttgtataagg tttttctacc aacactttgc ggcttccata tcggcttcaa tctgcctttt
     3421 cagttctgcc ataccgtcaa acttttcctc atcgcgcagt ttgtgcagga agcggacgtt
     3481 caaccgttgt ccgtacaaat cgccttgaaa gtcgaacagg tggacttcaa gcttttgaga
     3541 acagccgcta tcaacggtgg gattgaaacc gaagctcgcc acgccgcgcc gcgtgccgaa
     3601 tgcgccgtcc gcttcgacga caaacacgcc gccgagtgca taacggtggc ggggcaggcg
     3661 gatgttggca gtcggggcgt ttaaggtgcg tccgagtttt ctgccgtgca ccaccctgcc
     3721 gctcaagacg tagtcgtgtc ccaaaagttt tttcgcatag gcaaggttgc cgtctgaaag
     3781 ggcttgtcgc acggcggtac tgctggtgcg gatgtcttcg acgatgacgg aaggcgtacg
     3841 ctcggtctgc atatcgggct gttgtgccaa aagttcaaaa cagccttccc gccccgcacc
     3901 gaaacggaaa tcgtcgccga cgagcaaata acgcgtattc aaggtttgac gcagcaggcg
     3961 gtcgataaac ccttgcgcgg atatttcgga aaaattttga tcgaaacgta aaacccagac
     4021 ggcatcgacg cagcccgtcc cttccagcaa ttccagtttg gtacgcaggg ggctgatgcg
     4081 gcagggcggg gttttgcctg cgagggcggc aaaaaattct ttgggttgag gttcgaagat
     4141 gacggcaacg acgggcagtc cgcgcgtatc ggcttcgagg cggagttttt ggaggatgtg
     4201 tttgtgtccg aggtgtacgc cgtcaaaatt gcctatggtt acggcggccc cgtgcggaaa
     4261 gtcgggcgcg ttgtgccgcc ccagcctgat tctcattgtt atgttcgggt atgtggtgaa
     4321 acaggcggtc attgtaaacg gtattgcggt ttatagacag tgtgccgccc gttacgcccg
     4381 cccgacgcgg gaaaagtagg caaatttccc gcagccgaac gcgccaaacg cataaagcgc
     4441 gcagcaggcg cggtgctatg tgttgaaaca tcgccccaaa cctgatgcaa catcagtgaa
     4501 aatcgttctt ttttaaccag gtaaaccgag acaaacaacc ctccgccgtc attcccgcgc
     4561 aggcgggaat ccggacctgt ccgcacagaa acttatcgga taaaaacagt tgctcaaacc
     4621 ccgagattct agattcccac tttcgtggga atgacggttc agttgcgttc cgacaacacc
     4681 gtaatcttga aactcgtcat tcccgctcag gcgggaatct agaacgtgga atctaagaaa
     4741 ccgttttgcc cgataagttt ccgtgcggac aggtccggat tcccgcctgc gcgggaatga
     4801 cggcatttct gcggcaatcg gattatttcc aaaccaaaag cgcgtgattg cgtttgccgc
     4861 gccgaaggat ggtgtatttg ccgaaacgtt tgtgttcgtc ggtcagcagg caggcatcgt
     4921 cggggcgttc ggcggcgtgg ttggggttgt tggcttcggc aggtttgccg ttgagcaaaa
     4981 ccgctttgct gttcacaaag ccgcgcgctt ctttattgga ggatgccaaa ccggttttga
     5041 ccaaggcttc gacgacattg atgccgtctg aaacttcaaa tgcaggcagg ccgtcgaggg
     5101 cgagctgctc gaagtcgctt tcggtcaggc tgctttggtc ttcggcaaac aggctttcgg
     5161 aaatgcgctg cgcggcggca agggcttctt cgccgtgaat caggcgggtc atttcttcgg
     5221 cgaggatgcg ttgcgcttcg ggcttgctgc cgcttgcctg gtctttggct tcgacggcat
     5281 cgatttcttc gatggacagg aaggtaaagt atttcaggaa tttatacaca tcggcatcgg
     5341 cgactttcag ccagaattgg tagaactgat agggcgaggt ttttttcgcg ttcagccata
     5401 ctgcgccgcc ttcggttttg ccgaatttgg tgccgtctga tttggtaacc aaaggcagag
     5461 tcagtccgaa cacttgtttt tggtacaggc ggcgggtcag gtcgataccg gcagtgatgt
     5521 taccccattg gtcggagccg ccgatttcca aaaccgcgcc gtggcgtttg ttcaactcgg
     5581 cgaagtcgta gccttgcagc agggagtagg cgaactcggt gaaggaaatg cctgcgccgt
     5641 cgcggtcaat gcgctgtttg acggattctt tgttcagcat ggcgttgacg gagaaatgct
     5701 tgccgatgtc gcgcaggaag tcgaggcagt tcattttgcc gaaccagtcg gcgttgttcg
     5761 ccataatggc ggcgtttccg ccttcaaagc tcaagaaagg ggttaattgg ctgcggatgc
     5821 tttccaccca gccggcaacg gtttcggcgg aattcaagct gcgttcggcg gctttgaagc
     5881 tggggtcgcc gatcataccg gtcgcaccgc ccaccagtgc aatcggcgta tgccccgcct
     5941 gttggaagcg gcgcaatgcc aatacgggca gcaggtgtcc gatgtgcagg ctgtcggcgg
     6001 tcgggtcgaa gccgcaataa agggaaattt tttgttcgtt caacaaagcg tctaaggctt
     6061 cgatgtcggt ggtttgcgcg ataaggccgc gcgattgcag gtcttggatg acgctcatcg
     6121 gtctctttca aaaaaaatta gcggttttgc aaaccgccga ttgtaacaaa tttaagcgaa
     6181 tcaatggtta tggcgcgtat cgagaaaccg ttgtttttcg gaaaaacgct tcgccaattc
     6241 cgtgccgccg taagggttga tgtggtcttt gtccgagtaa accggcaatc cgccgatttg
     6301 gaaatttgcg gggatatagg cggcggcatc aataatatag acgttggggt atttggctgc
     6361 caattccctg atgcgtgcat tggctttcag agtgctttcg tcgtccgggc gcagggtttg
     6421 gcggtaaccc ggtatgcgtg aagacaagat ataggcgcgc tggacgttgt aagacgaggc
     6481 aaggttgtcc gccatcaggt aaacggcttg tttttcggac gagagtttat gcagcatacg
     6541 gtcgaatttt ttgaaaaaac cggcatcgta ggcaagggag cggctgtttt cgggcatttg
     6601 gctgccccag cgcatcgcca aaaccacttt tgaataccgg ggcaggtgtt cttcggcata
     6661 gcggtaaacg gcgcggcagg cggcccagtt ttggaatacg cgggacgcgt agccttccac
     6721 ataggcgcag gcgtcggcgg aaaccatagt ggcagcccat ttttcttttt tgcccacggc
     6781 atcgaagaat gttttgtaat ggtcggcgtg ggagtcgccc aaaaccagca gttccggctg
     6841 tttttccgta tccccccata ggcattgttt gccggtattg ttgtggcagg aggtgttgga
     6901 acgcgtcagc cccaagcggt cgtattgcgc cataaacggc agcctcatcg caaaaaatga
     6961 gcccgccccc aaaatgagca taggcaaggc ataaatccat aaaacggatt gtgcgaacga
     7021 accttgccat tttttaaacg gtttttcgat gcagtggtaa gaaaacaggg aaagcagcaa
     7081 tatcaggacg accgccgccg ccggcgaata aggcggcagg ttgtccgggc cgatatagcg
     7141 cataaaggcc aatatcggcc aatgccacag ataaagcgaa taggaaatca aaccggcggc
     7201 aacggtgatt ttccattgga aaaatttttt aagcgggtgt tcgtaatgat tgaaataaat
     7261 cagcgcggca acagccagac agggaatcaa agcggcgggc cctgggaaat aggcggtttg
     7321 ttccgaatag gcaaacaggc aggcggacag tatgcacgcg gaaaacaatg cgccggcggc
     7381 ggcacagcat ctgccgacgg cgagattccg ctgttgccgg taccgcatcc acacggcggt
     7441 cagcgatccg accagcattt cgcaggcgcg caggtggggc aggtaatatt tatcgaacgc
     7501 ggaaggcata aaggaagcgg caaggcttaa ggcgcacaat gcggcgagga agccgaactg
     7561 tacgcgcggg cttttgcggg cgacaagcag cagcaatatc ggaaagacaa agtaaaattg
     7621 ttcttcgacc gacaaagacc aaatgtgcag caagggcttt tcttcctgcg cgggatcgaa
     7681 ataatccttc ccccttgcaa aatacaggtt ggaggcgaaa cccaaggcgg tcagcgcgga
     7741 tttccacaaa agaaagaaat catctttggt gaataaaaag aagccgcctg ccagcgttgc
     7801 cgccaatacg gcgaaaaatg cgggcaaaat ccgtttgatg cggcggatat aaaatgcctt
     7861 cagggaaaac ctcccccccc cgacatttcg cgaaggagga tcgccgtcat caaaaagcct
     7921 gaaatcacaa agaatatatc gacaccgaga aatccgcccg gcagccaatt tttttcgata
     7981 tggaacacga tgacggacaa aacggcggcg gcgcgcaatg tgtcgatgtc cgggcggtag
     8041 ggtaaggctt ggctcataat atttttgctt tcatcgcggc ggcgcaaatg ccatctgaaa
     8101 ggtcattttg tatcggggct cgggatattc ggaatgccgg acggattttt gcaacagcgg
     8161 ggcaggcggt ttgttttgca ggaatcgggg agggcaaatc ggaaatgcgg acgggatttt
     8221 attttgatgc agccgcattc cggcggtatg gaaacgccga aaaccaccaa aaccggcttc
     8281 agacggcatt tctggcaaag ccgtctgaaa acctgccgca tttgggttac acgttaaaca
     8341 aaaagtgcat cacatcccca tcctgcacga cgtattcctt accttccaca cgcattttgc
     8401 cggcttcttt ggctttggct tcgccgccga gcgagacaaa gtcgtcgtaa gaaatgactt
     8461 gggcgcggat gaagccgcgt tcaaagtcgg tgtgaatcac gccggcggct tgtggtgcgg
     8521 tgtcgccttt gtggatggtc caggcgcgga cttcttttac gccggcggta aagtaggttt
     8581 gcaagcccaa gaggtcgtag ccggcgcgga tcaggcggtt caggcccggt tcttccaagc
     8641 ccatttcggc gaggaattcg gctttttcgt cctcttccaa ttcggcgatt tcgctttcca
     8701 ttgcggcgca tacagccaca accggcgcgt tttcttttgc agccaattct ttcaggcggt
     8761 cgaggtgcgg gttgttttca aaaccgtctt cggaaacgtt gccgacatac atggccggtt
     8821 tggcagtcag caggaacagc ggcttgagca tggcgagttc ttctgcgtcc aaaccgaagg
     8881 agcgtacagg cttgccttcg tccagatgag gcagcagttt tttgcacaga tcgaccagtt
     8941 tttgtgcgtc tttgtcgcct gagcgtgcgc gtttttcttc gcggacgatg gctttttcga
     9001 cgctggccaa gtcggcaagc gccaactccg taccgatggt ttcgatgtcg gcaatcggat
     9061 cgacgcggcc tgcaacgtgg acgatgttgt cgtcgtcaaa gcagcgcacg acattcacaa
     9121 tcgcatcggt ttcgcggatg ttggcgagga actggttgcc caagccttcg cctttgcttg
     9181 cacctgcaac caaaccggcg atgtcgacaa attcgacgat ggcaggctgc attttttgtg
     9241 gattgacgat ttttgccaat tcagccatac gcggatcggg gacttcaacg atgccgacgt
     9301 tgggttcgat ggtgcagaaa ggatagtttg ccgcttcgat acccgattgg gtcagcgcgt
     9361 taaagagggt ggatttgccg acgttgggca aaccgacgat gccgcatttc aaactcatgt
     9421 tttttcctga aaatagagaa atttaacggc ggattatagc ataccgccgc tcgcgttccg
     9481 aaaaaatgcc gtctgaaacg gcttcagacg gcatccggtt tcaaaaaccg ctcagaacaa
     9541 gccgtgaatc acgccgtgtt cgtccacatc gattttctcg gcggccggca ctttcggcag
     9601 acccggcatc ttcatcatat tgccgcacag cgcgacgatg aagcccgcgc cggcggaaac
     9661 ggtgatgccg cgcacggcga tgcggaagcc ttcggggcag cccaacagtt tggcgttgtc
     9721 gctcaaagaa tattgggttt tcgccatgca aatcggcatt ttgtccaagc ccagtttttc
     9781 cagcgaggcg atttcggcag acgcttccgc gctgaaatca acatcttccg cgccgtacac
     9841 tttttgggca atcacacgga ttttgtcttt gatgcccaac tcgacatcgt aggcgaaacc
     9901 gaagttattg gtttgacttt cgatggcgtt gacgactttg tgcgccaaat ccgcgccgcc
     9961 cgcaccacct ttgccccaca cttcggtcag ggaaacttca acgccgtgtt cggcacaggc
    10021 tttttcaatc atcgccaact cggcatcgga gtcggacacg aagcggttga gcgcaacgac
    10081 gacgggcagt ccgaatacgt ttttcaggtt ggaaatgtgt ttcaagagat taggcaggcc
    10141 ttttgccaag gcttcgaggt tttcttcgcc gaggttggcg cgttccacgc cgccgttgta
    10201 tttcaaagcg cggacagtgg cgacgacaac agccgcatca ggtttcaaat ccgccagacg
    10261 gcatttgatg tcgcagaatt tttccgcgcc caaatctgcg ccgaagcctg cttcggttac
    10321 ggcataatcg gcaagatgtt tcgccaaacg ggtggcggtt acggagttgc agccgtgggc
    10381 gatgttggcg aacgggcctc cgtgtacgaa ggcgggcgtg ccttcgatgg tttgcaccaa
    10441 gttgggctta atcgcatctt taagcaatgc cgccatcgcg ccgtgtgcct tcaaatcttt
    10501 ggcataaacg gggctgccgt ctttggcgta ggcgacgagg atgttgccca aacgctcttt
    10561 caaatcgctg atgtctttgg caagacagaa taccgccatc acttcggaag caacggtaat
    10621 gtcgaaaccg tcggggcgca tcacgccgtc aacaggcttg cccatgccgt cgatgatgtt
    10681 gcgcaactgg cggtcgttca tatcgaccac gcgccgccac aggacgcgtt tcggatcgat
    10741 gttcaactcg ttgccttggt agatatggtt gtcgagcatg gcggcgagca ggttattcgc
    10801 cgcaccgatg gcgtgaaagt cgcctgtgaa gtgcaggttg atgtcttcca tcggcaaaac
    10861 ttgggcatag ccgccgcctg ccgcaccgcc tttcacgccg aacaccggac ccaaagaagg
    10921 ctcgcgcaaa gcaatcacag agtctttgcc gatatggcgc aacgcgtccg ccaaaccgat
    10981 ggttacagtg gttttaccct caccggcagg agtcggatta atggcggtaa ccaaaatcag
    11041 acggccttgt ttttgcggaa gcttgaatgc ctcggcagga ttgattttgg ctttgtaatg
    11101 gccgtagggt tcgatattgt ccacgttcaa acccagcttg gcggcaattt caccaatcgg
    11161 acgcatggtg gaggattggg cgatttcggc atcggttttg aagctcatga ttttccttta
    11221 gaaatgagga gggacatgcc gtctgaaagc atcaggcgac aaacaggtgg attgaaaata
    11281 atatcaggca tattataacg ttatccgcac caaacccgca gtgaaatttt tgacgctgca
    11341 acaaaaatac cgttcatatt gttcacaatc caaggagaaa acatgagcag caacgcatgg
    11401 ctgttttggg cattggcatc ggcaggcttc gcctcattga ccgccatctt cgccaaaatg
    11461 ggtttacagg gtatagattc agatttcgct accttcatcc gcaccttggt catcctcgcc
    11521 gctttgttat tgtttttgac ctacaccggc aaatggcagg gtgtgaacgg ctttacgggg
    11581 cacaactgga cattccttat cctatccggc cttgctaccg gcgcatcttg gcttgcctac
    11641 ttcaaagccc tgcaactggg taacgcctcg caagtcgccc ccgtcgacaa attcagcctg
    11701 gtcttggtcg cgctgatggc ggtggttttc ttggacgaac gcccgaacac gcaggaatgg
    11761 ataggcttgg ggctggtaac ggcgggcgtg ttggtgctgg cgttgaaacg ttaaaccgaa
    11821 tccgccatac cgcctgaaac cgggttttga caatctgacg ggcggcggtc tgaacaaagt
    11881 tacccaataa aatccgacac cgcgccgacg gcaaaccgcc gcgcttcaga tggcatttgt
    11941 tggacggaaa gccccgaacg ccctattttc acgcctccac gaaaatatgc tggacatccg
    12001 gtcggaaccg ttcggcagcc ggctcggcaa gccaccgttt tatttcactt ccaagcccct
    12061 gcaagggctt gagcctcttt cagacggcat accgtgccga catccagcca caagcccgta
    12121 tgcttctgac cgctcacgcg gttttgccgc atttcgccac gcaatacggg cgcgagtttc
    12181 gccacactgc ccgcttcgat tccgtcaaac atttcaggac ggtaaatacc cacgccgcta
    12241 aatgtcaatc cgttgccgcc atttacttcc ggccgcacgc tgccgtcggg cagcagggaa
    12301 aaatcgccgt cggggttgtg cggcggattt tccaccagcc acagatgggc ggaaatatgt
    12361 tccggcaggg acgatgccgt ctgaaacgcg gcggtaaaat cgatgtcggt cagcacgtcg
    12421 ccgttgacca ccaaaaacgg ctgcccaccc aacagcggca atgcctgcgc gatgccgcct
    12481 gccgtttcca aaccgcctgc gggttcgggc gaataggcga tgttcacgcc ataagccgag
    12541 ccgtcgccca aagcatcttc tatctgccga cccagccaag cgtggttgat gacgatttcg
    12601 gtaaaccccg cctgcttcag acggcatagg tgccaaccga ttagaggctt acccgccaca
    12661 tcgagcagcg gcttcggagt ggtatcggtc aaagggcgca tacgctcgcc gcgtcctgcc
    12721 gccagtatca tcgctttcat atatctgtcc gaatatcagt ctaaaaatct aaactgccgt
    12781 ctgaaataca gcagcgcggg gcgtttgcac ccgcagtttt tgatttcgtc gagcctgacg
    12841 taaaacacaa aatgcgtgcc gatttcatgt ttgccgacaa tatgcccgtg caggtgcacc
    12901 aacgcgccct ctatttcaag ttgtcccgtt ttgccgcgat gccagatgtg gtaggcaaac
    12961 cgctcttcgg gcgacaggcc ggtcagcccg gcaaaatgtt cggcaacatc ctgatgttcg
    13021 tccgccagcg tattgatgca caggctgccg ttttccgaca ggatcggaat gatacgggct
    13081 ttacggttga tgcacagcat cacggtcggc ggctcgtcgg taaccggcgc gaccgccgtc
    13141 attgtaatgc cgtaacgccc tgccgcaccg tctgtcgtga tgacatgaac gcctgccgca
    13201 caggatgcca tcgcatcacg gaacgaagtt tgaaaagttt tctgcaaatc cgccattttt
    13261 cccctttaaa ctgtccccta tataagaatg ctgcacacaa ggcacccccc atgtgcagca
    13321 gttctgattc aaaaagccgt cggtcggaca tttccgcgcg ttacggcgta ttacgagttc
    13381 aacgcatcct cgattttggc aagttctgcc aacaggtctt taagcagcag cattttctcg
    13441 cggcccagca cttcctcgat agcgtcgtaa cgctcgtcca cttcttcgcc gatttcctca
    13501 tacagcttct cgccctcggc agtcagcttc agaaaaacac gtcgttggtc gttggaaggt
    13561 ttcaggcgga cgaccaaacc cgctttttca aggcaggtca ggataccggt caggctgggg
    13621 cgcagaatac acgcctgatt cgccaaatct tgaaagtcca gcgtgccgtt ttccgccaaa
    13681 agacggataa tccgccattg ctgatcggta atattcgcct gattcagaat aggcctgaat
    13741 tgggtcatca gggcttccct tgcctgtatc agactgatat tgatagacgc atgttttgat
    13801 tgggtaggca ttgttcaagt ctccaagtta tcgaaaatca aactttcaaa ccgtcgggaa
    13861 agcctgtggg cgtaaatttt gatgcaaccg ttatataaca aaacgaacat atagcaacaa
    13921 tacgctataa accgcatcgg acgactgggt ataaaagact ttaattccga taatcctatc
    13981 taaaaatatt ttaatagtta tatcttagtc tattttccct acaatctcaa caaagtatta
    14041 catttactgt cgaaagaact ttcttccaga aaaaacaaaa gccgccgcat cagacgagca
    14101 aggtatatgc cgtttgattc tctacatagc gtaaagttta acaaaaacaa aagttaaccg
    14161 aaaacatccg cctgaaaaat tcgtgcgcgc aagccccaat aactgctgat tcccgtcgta
    14221 taatggacca ttttcccatt tttgaccaaa acgacggcag gcgttgcgac aatccgccaa
    14281 gaccttgcca aacccccgtc ctcatcgttg acagtcggaa agcccaagcc gcgttttgcc
    14341 atatacgccg ccacttccgc cgaactgccg gaacgtaccg ccacgccgac gaccggcacg
    14401 ccgtccgccg ccaaatcatc gattatcggc gactgataac ggcacacgcc gcaccagctc
    14461 ccccaaaaat acaccagcac cgtcctatcc cggctaaact gtcccaaagt cagccgctgc
    14521 cccgacagca gggtcaaagg ccgccctgcc gcgccggcag gttcgtcggg cttgcgtacc
    14581 caatccaaaa acagcgacac caataaaaac accaatgccg tctgaacggc aaatttgatg
    14641 cccgaaagca gtttcttttt catacgctct ctcaaacggt acgcccgcgc aaccggcagg
    14701 caaacaaaaa gccaagtctc aaaacttggc ttccggttat ctggtgggtc gtgagcgatt
    14761 cgaacgctcg accaacggat taaaagtccg ctgctctacc gactgagcta acgacccgat
    14821 aagccgtgca ttatacagca ccatcctacc tcgtcaagca aattttacag gcttaattgc
    14881 agaccactgt ttgcacggga tattttgaca acggattttc acaatccgcc gcataccgtg
    14941 taaaagttcg cacaaggaaa agcaaaccgc ccgaaatcaa tgtacacttt ccgcccgttt
    15001 cccaaactgc acacagaaac acacattatg aacatacaaa acatccgcac cctcctcgac
    15061 accgtcgccg ttccgaatac ggcacgcacg ctcggcggcg aaaaggccgt ccgttcggtc
    15121 gaacagcgtt cagacggcat ccatatcgcc ctgcatttcg gcttccctgt cgcgcacatc
    15181 gccgccgccg tcgccgattc cgtacaagaa atcctgatgc ccgaaacagg cggcgcgcac
    15241 atccatctgt ccatagacac cgaaatcggc acacataaag tccaacctgg cgttaccacc
    15301 atcaaaggtg tgaaaaacat cattgccgtc gcctccggca aaggcggcgt gggcaaatca
    15361 acaaccaccg ccaaccttgc cgccgcaatg gcgcggatgg gcgcgcgcgt cggcgtgctc
    15421 gatgccgacc tttacggccc gagccaaccg accatgttgg gtgtggacga ccgcaaaccc
    15481 gatcagaaaa accaaaaact cattcccgtc gaatcttcag acggcataca ggtcatgtct
    15541 atcggctttc tcgtcgatac cgaccaagcc gtcgtctggc gcgggccgat ggtcagccaa
    15601 gccttgcagc aactgatgtt tcaaagcgag tgggacgaag tggactacct gtttatcgac
    15661 ctgccccccg gcacgggcga catccaactc acgctgtccc aacgcatccc cgtaaccgga
    15721 tcggtcatcg taaccacgcc gcaggacatc gccctgatag acgcgcgcaa agccgtggat
    15781 atgttccgaa aagtcaacat tcccatttta ggtgtattgg aaaatatgtc cgtccacatc
    15841 tgctccaact gcggacacag cgaagcactg ttcggcacag acggcggcaa agacttggcg
    15901 gcacgcctca acgtccccct gctgggacag cttccattaa gcctgcccgt gcgcgaagcc
    15961 atggacggag gggcgacggc gcaactgttc gacgaacacc ccgccatcgc ccgaatctac
    16021 accgatgccg cattccaaat cgccctgggc attgccgaca aaggcaaaga cttcagcagc
    16081 cggttcccta aaatcgtcgt cgaataaagc cgcgtccgaa accgcaacag caatgccgtc
    16141 ccaagccccg cgcctgccga tatgccgtca ttcccgcgca ggcgggaatc tagaacgtgg
    16201 aatctaagaa accgtgttgt aaaggcagac cgatgccgtc attcccacga aagtgggaat
    16261 ctagaacgta aaatctcaag aaaccgttat acccgataag tttctgcacc gacaggtctg
    16321 gattcccgcc tgcgcgggaa tgacggttcg ggtatttctg acgatttagg cattcccgac
    16381 aaggtggatt ttcaaggtgt tgtatagggt gtaggaggat tcgtaaaagg tgagttatag
    16441 ggtgggcttc agcccaccga ttccaacgat tccaccaatc ctacaccgtt cccatagact
    16501 caaatcaaca cagaaactta tgcgccgtca ttcccgcgca ggcgggaatc tagaccttag
    16561 aacaacagca atattcaaag attatctgaa agtccgggat tctggattcc cactt
//
LOCUS       DAWFLT010000036        16589 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWFLT010000036 DAWFLT010000000
VERSION     DAWFLT010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16589
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.036"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            209..1273
                     /locus_tag="WGR74_001189"
     CDS             209..1273
                     /locus_tag="WGR74_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ0905614.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1414..1737
                     /locus_tag="WGR74_001190"
     CDS             1414..1737
                     /locus_tag="WGR74_001190"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ0905615.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1809..2132
                     /locus_tag="WGR74_001191"
     CDS             1809..2132
                     /locus_tag="WGR74_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ0905616.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGRQEVFHLHIHI
                     MGTPV"
     gene            2179..2382
                     /gene="tatA"
                     /locus_tag="WGR74_001192"
     CDS             2179..2382
                     /gene="tatA"
                     /locus_tag="WGR74_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ0905617.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2386..3072
                     /gene="tatB"
                     /locus_tag="WGR74_001193"
     CDS             2386..3072
                     /gene="tatB"
                     /locus_tag="WGR74_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ0905618.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMEGNLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPFPDAANTLLDGISDVMPSERSYASAETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDLRPKSR
                     AKPKLRVRKS"
     gene            3086..3856
                     /gene="tatC"
                     /locus_tag="WGR74_001194"
     CDS             3086..3856
                     /gene="tatC"
                     /locus_tag="WGR74_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ0905619.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGMMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKISVVTTGQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIRPPATT"
     gene            3909..4514
                     /gene="maf"
                     /locus_tag="WGR74_001195"
     CDS             3909..4514
                     /gene="maf"
                     /locus_tag="WGR74_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ0905620.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYRVIQLPAGIDESVKAGETPFAY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGNAENRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            4530..4883
                     /locus_tag="WGR74_001196"
     CDS             4530..4883
                     /locus_tag="WGR74_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ0905621.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLAWELWIAPLRPGGSWLALKTLP
                     LCLPLSGILKKKIYTYQYSSMLILIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4873..6687
                     /locus_tag="WGR74_001197"
     CDS             4873..6687
                     /locus_tag="WGR74_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ0905622.1"
                     /translation="MPAETPVSGARPAAKLPIYILPCFLWIGIVPFTFALRQQPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPPISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     FQNIIVYSGQGVIGHIGQRNNLGHYLMWGILASAYLNGQRKIPAALGVICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLSIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIHDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKHPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKALKYRPYSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6877..7554
                     /locus_tag="WGR74_001198"
     CDS             6877..7554
                     /locus_tag="WGR74_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0905623.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7568..8974
                     /locus_tag="WGR74_001199"
     CDS             7568..8974
                     /locus_tag="WGR74_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ0905624.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9092..11518
                     /locus_tag="WGR74_001200"
     CDS             9092..11518
                     /locus_tag="WGR74_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ0905625.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLPAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSSDWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALEAADPALIAEAL
                     HLPPYRHTTHNAVQFQFGSHSLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTVIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     HPKTLSLKNDTTVTITPILPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLHTDHKIT"
     gene            11563..11808
                     /gene="rpsP"
                     /locus_tag="WGR74_001201"
     CDS             11563..11808
                     /gene="rpsP"
                     /locus_tag="WGR74_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ0905626.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11824..12333
                     /gene="rimM"
                     /locus_tag="WGR74_001202"
     CDS             11824..12333
                     /gene="rimM"
                     /locus_tag="WGR74_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ0905627.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNSELQVKFEGINDRDLAFSLHGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            12333..13082
                     /gene="trmD"
                     /locus_tag="WGR74_001203"
     CDS             12333..13082
                     /gene="trmD"
                     /locus_tag="WGR74_001203"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ0905628.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHPKAAEL
                     AELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRSLIPKESRILNKILQEQREIQS"
     gene            13098..13463
                     /gene="rplS"
                     /locus_tag="WGR74_001204"
     CDS             13098..13463
                     /gene="rplS"
                     /locus_tag="WGR74_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ0905629.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13483..13788
                     /locus_tag="WGR74_001205"
     CDS             13483..13788
                     /locus_tag="WGR74_001205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905630.1"
                     /translation="MPSEPSDGISYCRNIRNGTAPRSTPLPAHPESPSEPNKIYRPNV
                     IQYHISHIIITALPLSLSKPQIIMTPSLLLSGLTFRLVLALIAVSLLWGVYFWAVSA"
     gene            13785..14510
                     /locus_tag="WGR74_001206"
     CDS             13785..14510
                     /locus_tag="WGR74_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ0905631.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFR
                     GINAVQKQRVHESLERVGMRQFAGRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPEWCAS"
     gene            14534..15409
                     /locus_tag="WGR74_001207"
     CDS             14534..15409
                     /locus_tag="WGR74_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ0905632.1"
                     /translation="MNLYDLLLAPFAEFDFMCYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSTLTLLTLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15486..16400
                     /locus_tag="WGR74_001208"
     CDS             15486..16400
                     /locus_tag="WGR74_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ0905633.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQNLVGANQDTHAYHMTSGDIKKIRSAKLVLINGLGLEAADVQRAVKQSKVSYTEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEYDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNVKALTNAMKQ"
BASE COUNT         4334 a         5288 c         3641 g         3326 t
ORIGIN      
        1 ccgtctgaag ccgattcggt tttcagacga catcattatt tgacctgacg caaacagaaa
       61 gccttccgcg tgcttaaaca catctcttgt agtaaaatat ctaaacagat tcaactggaa
      121 acggtcaggc ttcttcacgc cattcccatt tcaaccgccg cctgccagat tcagacggta
      181 ttcctacttg aaaacaagga gcgttacaat gaaagcagca cgtttttacg ataaaggcga
      241 catccgcatc gaagacatcc ccgaaccgac cgtcgccccc ggcactgtcg gcatcaatgt
      301 cgcctggtgc ggcatctgcg gtactgacct gcacgaattc atggaaggcc cgattttcat
      361 tccgccttgc ggtcatccgc acccgatttc cggcgagtcc gcacccgtaa cgatgggaca
      421 cgagttctcc ggcgtggtct atgccgtcgg cgaaggcgtg gacgacatca aagtcggcca
      481 acacgtcgtg gtcgaaccct acatcatccg cgatgacgta ccgaccggag aaggcagcaa
      541 ctaccacctc tccaaagata tgaactttat cggcttgggc ggctgcggcg gcggtctgtc
      601 cgaaaaaatc gccgtcaaac gtcgttgggt gcatccgatt tccgacaaaa tcccgttgga
      661 tcaagccgct ttgatcgaac cgctgtctgt cggacaccac gcctatgtac gcagcggcgc
      721 gaaagcaggc gacgtcgcat tggtcggcgg cgcaggtccg atcggtttgc tgttggctgc
      781 cgtgttgaaa gccaaaggca tcaaagtcat catcaccgag ttgagcaaag cacgcaaaga
      841 caaagcgcgc gaatccggcg ttgccgacta catcctcgac ccgtccgaag tcgatgttgt
      901 cgaagaggtg aaaaaactga ccaacggcga aggcgtggac gtggcattcg aatgtaccag
      961 cgtgaacaaa gtgttggata ctttggtcga agcctgcaaa cctgccgcca atttggttat
     1021 cgtatccatc tggagccacc ctgcaaccgt caacgtccac agcgtcgtga tgaaagagct
     1081 ggatgtgcgc ggcacgattg cctactgtaa cgaccacgcc gaaaccatca aactggtcga
     1141 agaaggcaaa atcaacctcg aacccttcat cacccagcgc atcaagctgg acgaactggt
     1201 ttccgaaggc ttcgagcgtc tgatccacaa caacgaatcc gccgttaaaa ttattgtgag
     1261 tccaaacctg taagcaaatt acatttgcaa cagcacaaat gccgtctgaa acgctcagac
     1321 ggcattttcc aacgaagcgc aacaggcgta ttgcccgaga gcagccgttt agcctatcat
     1381 tccgaacaaa cagaaacaaa cagaaggaaa atcatgggag attccgtact atccgccatc
     1441 caacaaacca tcacccagcg aaaatctgcc aatccgtccg aatcttacgt cgcacagctc
     1501 ttgcataagg gcgaagacaa aatcctaaaa aaagtgattg aagaggcggg cgaagtgttg
     1561 atggcatcca aagacaaaaa cccgtcccac ctggtttacg aagttgccga cttatggttt
     1621 cacaccatga ttcttctgac acaccacgac ctgaaggcgg aagacgtatt ggacgaactt
     1681 gcgcgccgcc aaggtttgtc gggcttggcc gaaaaagccg ctcgcacaga atcttgaatt
     1741 tatattaaaa tccgcacttt cccacattca atccgtctga ccgctgttca gacggcatcg
     1801 gagccgttat ggacaactgt attttctgca aaatcgccgc caaagacatt ccggcgcaaa
     1861 ccgtctatga agacggcgaa atggtttgtt tcaaagacat caaccccgct gctccggttc
     1921 atctgctgct gattcccaaa gtccatttcg attcgttggc acacgccgcg cccgaacatc
     1981 agcccctttt gggaaaaatg atgctgaaag ttcccgaaat cgccaaagcg gcaggactgg
     2041 cagacggctt caaaaccctg atcaacaccg gaaaaggcgg cagacaagag gtcttccacc
     2101 tgcatataca catcatgggc acacccgtat aaaccgttat ttcacaatca acccctaata
     2161 cttacttaag gatacatcat gggcagtttt tctctgacgc actggattat cgtactgatt
     2221 atcgtcgttt tgatattcgg caccaaaaaa ctgcgcaacg tcggcaaaga cctcggcggt
     2281 gcggttcatg acttcaaaca ggggctgaac gaaggtacag acggcaaaga agcccaaaaa
     2341 gacgatgtaa tcgaacacaa aaaagacgaa gacaaagcgt aatttatgtt tgatttcggt
     2401 ttgggcgagc tggtttttgt cggcattatc gccctgattg tcctcggccc cgaacgcctg
     2461 cccgaggccg cccgcaccgc cggacggctc atcggcaggc tgcaacgctt tgtcggcagc
     2521 gtcaaacagg aatttgacac gcaaatcgaa ctggaagaac taaggaaggc aaagcaggaa
     2581 tttgaagctg ccgctgctca ggttcgagac agcctcaaag aaaccggtac ggatatggag
     2641 ggtaatctgc acgacatttc cgacggtctg aagccttggg aaaaactgcc cgaacagcgc
     2701 acgcctgctg atttcggtgt cgatgaaaac ggcaatccct ttcccgatgc ggcaaacacc
     2761 ctattagacg gcatttccga cgttatgccg tccgaacgtt cctacgcttc cgccgaaacc
     2821 cttggggaca gcgggcaaac cggcagtaca gccgaacccg cggaaaccga ccaagaccgt
     2881 gcatggcggg aatacctgac tgcttctgcc gccgcacccg tcgtacagac cgtcgaagtc
     2941 agctatatcg ataccgctgt tgaaacccct gttccgcata ccacttcgct gcgtaaacag
     3001 gcaataagcc gcaaacgcga tttgcgtccc aaatcccgcg ccaaacctaa attgcgcgtc
     3061 cgtaaatcat aaagagagca atccggtgtc cgaaacacaa aacgaacaac ccgtccaacc
     3121 gcttgtagag catctcatcg agctgcgccg ccgcctgatg tggacggttg tcggcatctt
     3181 agtctgcttt ttcggcatga tgccgtttgc ccaacaactc tatactttta tcgccgaccc
     3241 gctgatggca aacctgccca aagacaccag catgattgcc accgatgtca tcgcaccatt
     3301 tttcgtgccg gtcaaagtta ccctgatggc ggcattttta atttcgctgc cgcatacgct
     3361 ctaccaaatc tgggcatttg tcgcgcccgc actctaccaa aacgaaaaac gcctgattac
     3421 gccgctcgtc ctctccagcg tcagcctgtt tttcatcggc atggcatttg cctacttttt
     3481 ggttttcccc gtcattttca aattccttgc cagcgttacc cctgtcggtg tcaatatggc
     3541 gacagacatc gacaaatacc tgtcttttat cttaggcatg tttgttgcgt tcggcacaac
     3601 gtttgaagtt cccatcgtcg ttatcctgtt aaccaaaatc agcgtggtaa caaccggaca
     3661 gctcaaacgc gcccgcccct atgtgattgt cggcgcgttt gtcattgccg ccatcatcac
     3721 gccgcccgat gtgatttcac aaaccctgct tgccattccg ctgattctct tatacgaagc
     3781 aggtatttgg ttcggacgct ttttcacgcc acgttcagaa caggatggcg acatacggcc
     3841 gcctgcaaca acctgacact atgccgtccg aacctccgcc tcataccgca acagattaag
     3901 gaataccttt gaataccctc tatttaggtt caaacagccc gcgccgaatg gaaatcctga
     3961 cacagttggg ctaccgcgtc atccaactgc ctgccggcat cgacgaatcc gttaaagccg
     4021 gcgaaacacc tttcgcttac gttcaaagga tggcagaaga aaaaaaccga accgccctga
     4081 ccctcttttg cgaaaccaac ggcacaatgc ccgatttccc cctgattacc gccgacacct
     4141 gcgtcgtttc agacggcatc atattgggca aaccccgctc ccaagccgaa gcaatcgaat
     4201 ttttaaaccg attgtccggc aaacaacata ccgtcctgac tgccgtctgc attcattatc
     4261 gcggcaatgc ggaaaaccgc gtccaaacca accgcgtcgt tttcaagccc ctgagttcgg
     4321 aagaaatttc cgcctatgtg caaagcggcg aaccgatgga caaagccggc gcctacgccg
     4381 tacaaggcat aggcggcatc tttatccaat ctatcgaagg cagcttcagc ggcattatgg
     4441 ggctgcccgt ttatgaaacc gtttcgatgt tgcaggattt gggataccgt accccacctt
     4501 tcatccgagc ctaacccgta aagaccgccg tgaacagaca aaccgcttac ctccttacct
     4561 ctttcagcct gatcgcactg ataatcctgt cccttgcttg ggaactgtgg attgcaccgt
     4621 tacgcccggg cggttcgtgg cttgcgctca aaaccctgcc cctctgcctg ccgctttcag
     4681 gcatcctgaa gaaaaaaatc tacacctacc aatacagctc catgttgatt ctgatttatt
     4741 ttgccgaagc cgtcatgcgc ctgttcgacg cctatccggc agaacaaatt tgcgccgcgc
     4801 tttccgcagt attcagcatc atcttcttca tatcctgcct gtcgttcgtc aaacaataca
     4861 aggaaacaaa caatgcccgc tgaaacgccc gtatccggtg cgcgccccgc cgccaaactg
     4921 ccgatttaca tcctgccctg cttcctttgg ataggcatcg tcccctttac cttcgcgctc
     4981 aggcagcaac cgtcgcccga cttttaccac gatgctgcag ccgcagccgg cctgattgtc
     5041 ctgttgttcc tcacggcagg aaaaaagctg tttgatgtca aaatcccacc catcagcttc
     5101 cttctgtttg caatggcggc gttttggtat cttcaggcac gcctgatgaa cctgatttac
     5161 cccggtatga acgacatcgt ctcttggatt ttcatcttgc tcgccgtcag cgcgtgggcc
     5221 tgccggagct tggtcgcaca ctacggacaa gaacgcatcg ttaccctgtt tgcctggtca
     5281 ctgcttatcg gctccctgct tcaatcctgt atcgtcgtca tccagtttgc cggctgggaa
     5341 gacacccctc tgtttcaaaa catcatcgtt tacagcgggc aaggcgtaat cggacacatc
     5401 gggcagcgca acaacctcgg acactacctc atgtggggca tactcgcctc cgcctacctc
     5461 aacggacaac gaaaaatccc cgccgccctc ggcgtaatct gcctgattat gcagaccgcc
     5521 gttttaggtt tggtcaattc gcgcaccatc ttgacctaca tagccgccat cgccctcatc
     5581 cttcccttct ggtatttccg ttcggacaaa tccaacaggc ggacgatgct cagcatagcc
     5641 gcagccgtat tccttaccgc gctgttccaa ttttccatga acaccattct ggaaaccttt
     5701 accggcatcc gctacgaaac tgccgtcgaa cgcgttgcca acggcggttt cacagacttg
     5761 ccgcgccaaa tcgaatggaa taaagccctt gccgccttcc agtccgcccc gatattcggg
     5821 cacggctgga acagttttgc ccaacaaacc ttcctcatca atgccgaaca gcacaacata
     5881 cacgacaacc tcctcagcaa cttgttcacc cattcccaca acatcgtcct ccaactcctt
     5941 gcagagatgg gaatcagcgg cacgcttctg gttgccgcaa ccctgctgac gggcattgcc
     6001 gggctgctga aacaccccct gacccccgca tcgcttttcc tgatctgcac gcttgccgtc
     6061 agtatgtgcc acagtatgct cgaatatcct ttgtggtatg tctatttcct catccccttc
     6121 ggactgatgc tcttcctgtc ccccgcagag gcttcagacg gcatcgcctt caaaaaagcc
     6181 gccaatctcg gcatactgac cgcctccgcc gccatattcg caggattgct gcacttggac
     6241 tggacataca cccggctggt taacgccttt tcccccgcca ctgacgacag tgccaaaacc
     6301 ctcaaccgga aaatcaacga gttgcgctat atttccgcaa acagtccgat gctgtccttt
     6361 tatgccgact tctccctcgt aaacttcgcc ctgccggaat accccgaaac ccagacttgg
     6421 gcggaagaag caaccctcaa agcactaaaa taccgcccct actccgccac ctaccgcatc
     6481 gccctctacc tgatgcggca aggcaaagtt gcagaagcaa aacaatggat gcgggcgaca
     6541 cagtcctatt acccctacct gatgccccga tacgccgacg aaatccgcaa actgcccgta
     6601 tgggcgccgc tgttgcccga actgctcaaa gactgcaaag ccttcgccgc cgcgcccggc
     6661 catccggaag caaaaccctg caaatgaccc gcgccggcgg atacggatac cgcccgaaat
     6721 gtaaagctcc atgcaagaca ttgcaaaaaa cagcaaaccg gtagggaaat acgctatcaa
     6781 aaaacattgc agccgtgtta agataaaccg tcaaacaatc ttttcacgcc ccgcccgaaa
     6841 cagggtcggg gcataccctt acgaaaagga aacaccatga gccgcgtatt actcgtagat
     6901 gacgatgccc tgctaaccga actgctgacc gaatacctga gcgccgaagg tctgaacgtc
     6961 cgcagcgttc ccgacgggga agcaggcgta caggaaatcc tgagcgggca gtacgatgta
     7021 gtcgtattgg attccatgat gcccaaaatg aacggcttgg atgtcttgaa aaacgtacgc
     7081 gcccgaagca ccgtccccat catcatgctg accgccaaag gcgacgacat cgaccgaatc
     7141 atcggcttgg aaatgggcgc ggacgactat gtccccaaac cctgcacacc acgcgaactc
     7201 ttggcacgca tcaatgccat cctgcgccgc gcacaacaca gcggcgaaca gaacaacgca
     7261 cccaacagca tctccgtcag cgatgtcgtc ctgtatcccg ccaaacgcca ggcatccgtc
     7321 aaagacatgc cgctcgaact gaccagcacc gaattcaacc tgctcgaagt cctgatgcgc
     7381 catgccggac aggtagtcag caaagaaacc ctgtccgtcg aagcactcga ccgcaagctg
     7441 gcaaaattcg accgcagtat cgacgtacac atctccagca tccgccacaa gttgggcgat
     7501 gcctctctga ttcaaaccgt acgcggcttg ggctacctgt ttgtcaaaaa ctgaaataaa
     7561 cagataaatg aaactgttcc aacgcatctt cgccacattt tgcgcggtta tcgtctgtgc
     7621 aatctttgtg gcgagttttt ctttctggct ggtgcagaac acccttgccg aaaaccagtt
     7681 caaccaacgc cgcaccatcg aaaccacttt gatgggcagc atcatttccg cattccgggc
     7741 acgcggggac gcgggtgcgc gcgaaatcct gacggaatgg aaagacagcc ccgtctcatc
     7801 gggcgtgtac gttatacagg gcgacgagaa aaaagatatc ctgaaccggt atatcgacag
     7861 ctataccatc gaacgcgccc ggcttttcgc cgccggacac ccgcattcca acctcgtcca
     7921 tatcgaatac gaccgcttcg gcgaagaata cctgttcttc accaaagact gggacaaact
     7981 ccaagcccgc cgcctgccca gccccctgtt gatccccggc ctgccgctcg ccccgatttg
     8041 gcacgaactc atcatattgt ccttcatcat catcgtcgga ctgctgatgg catatatcct
     8101 cgccggcaac attgccaaac ccatcagaat cttaggcaac ggcatggaca gggtggcaaa
     8161 cggagaactt gaaacccgta tctcccaaca ggtcgacgac cgcgacgacg aattgtccca
     8221 tcttgccatc caattcgaca aaatggtgga aaaactcgaa aaactcgttg ccaaagaacg
     8281 ccacctgctc catcacgtct cccatgaaat gcgttctccc cttgcgcgca tgcaggcaat
     8341 tgtcggactg attcaggcgc agccccaaaa acaggagcaa tatctcaaac ggctggaagg
     8401 cgaactgacc cgcatggata cgctggccgg ggaactgtta accctgtccc gtctcgaaac
     8461 ttccaatatg gctttggaaa aagaaagcct gaaactcctg cccttcctgg gcaacctggt
     8521 agaagacaat caaagcattg cccagaaaaa cggacaaacg gttaccctgt ctgccgacgg
     8581 aaaaatcccc gaaaacacaa ccatccttgc caacgaaagc tacctgtacc gcgccttcga
     8641 caacgtcatc cgcaacgccg tcaactacag tcccgaaggc agcaccatcc tgatcaacat
     8701 cggacaagac cacaaacact ggataatcga cgttaccgac aacggccccg gcgtggacga
     8761 aatgcagctc ccgcacatct tcaccgcttt ctaccgtgca gactccagtg ccaacaaacc
     8821 cggaacagga ctggggcttg cattgaccca acatattatt gaacagcact gcggcaaaat
     8881 catcgccgaa aacatcaaac cgaacggtct gcggatgcgc tttatcctgc ccaagaaaaa
     8941 aaccggttcc aaaacagaaa aaagtgcgaa ctgaccataa taccgtctga accggcttca
     9001 gacggcattg cacaaacagt taccccaaaa caaacacaca gcgggtgcat ctccaaaaat
     9061 cacgcacata ccgtccgcaa aggaatatat catgtcggca caaaccgatc cgggctactt
     9121 tttcatgccc aaccacatta ttctgatagg cgcgagcgaa cgtccgtaca gcctgggcga
     9181 acgcgtactc agcaacctgc tgagtacgcc ctttcaagga aaaatcaccc ccgtaaaccc
     9241 gcgccaccac accatagctg ggcttcccgc ctacaccagc ctcaacaaaa tccccggcaa
     9301 tgcagacctg attattgccg ttaccctacc cgacagttac gacaccctct tcaaaacctg
     9361 ccgcaaaaag cagctccgac acatcatcct catacaggac tgggacagcc tgcctgccgc
     9421 agaactgcac accgccgaaa ctgccatccg caaacaccac ggcaacggac tcaacatcac
     9481 cgcctgcacc accgcaggca tccaactgcc ctcactcgga cttaacatca gtacccaaga
     9541 cggatacgcc gcaggccata ccgccatact gaccggcaat gccgccgtca gccgccaaat
     9601 cgacaacatc ctgaacaaac tccgtcaagg cacatcccgc cacatcagcc tgcatcccgg
     9661 catcagcccc atcacttcct cagactggct caaccgcttc ggacacagcc tgcacaccaa
     9721 aaccgccgtc ctacaccaca accctgaaga ggatcagcgc aaactgttca gcgcaatccg
     9781 ccaatttacc cgccatacgc cgctgattct ccacatcacc tgccgcacga cagaaaccga
     9841 ccgtgccgta ctgcactgcc tcgcccgaca ctgcaacttc ctcatcagtt tcaacgccga
     9901 cgaccttgaa gccgcactgc gcgcccaact gtccgacctt ccacccctgt cccgactcga
     9961 catcctgtcc gacacgcctg ccgaatggct gaacgcgcac gcgccaaaaa ccctcgccct
    10021 ccactttccc aaccttcccc accacatccg caacggacac ctgaccggca cacccacacc
    10081 ctcaatctgc cacgacatcg cctcacgtca gcttgcccgc cccgacaccc aagccgtcct
    10141 gaccatcctc agcccctccg gacacgagga ttacaaaaaa acagcacgcg cccttatccg
    10201 cctgtccgaa cagaccgcca aacccctgct cgtcagcagc cccttttcag acggcataac
    10261 ccatttcgac acccccactc aggcaatccg caccctttcc taccgcaaca ccgccgccgc
    10321 cctgaaacag gcacagctcg acattgcacc gccgcaaccg tgccgtctga aaacaccgca
    10381 accccaaaac atcaaaaaag ccctcgaagc cgccgacccc gccctaatcg ccgaagccct
    10441 gcacctcccc ccctaccggc acaccaccca taacgccgtc caattccaat tcggcagcca
    10501 ctccctctat ggcgacatcc tgaccgcacg ttgcaacgga caaaccactg ccgtactccc
    10561 gccgtttacc acgctcgaca gccgccacct tgcccgcttt gccgaactcg acggcacaca
    10621 aaccctcgac cagttcctgc acacactgac cgtcattccc gaataccgcc aacacattct
    10681 cggcatcacc ctcaacctaa acggcggaca atacagcagc gatttcatct taaagacacc
    10741 cgaaacacac gacacgccca aacgcaagaa cacaggcaaa gccgcccaaa ccctcgaaca
    10801 tgccgccgca aaaatgcaga gtgccgccgc atacctgaaa cacaaaaacc cgacagccgc
    10861 cgaatttctc cgccacacaa gcgaagccgc cgcagaactg ctcggcagca aaaccgaaac
    10921 cggagcagcc gtacccaacg tacttgcccc ctatcccgca gcacacccca aaacactgtc
    10981 cctaaaaaac gacacgaccg ttaccattac ccccattttg cccgaagacg cagaagccaa
    11041 acagcagttc gtccgcagcc tcggtcccga agcacggtac acacgcttca tgacccacac
    11101 caacgaactg cccgcagcca cgttggcacg cctgtgcaac cccgattacc actgtgaagc
    11161 cgcatggacg gcaaaggatg ccgacagcaa catcgtcgcc gtcgtccgcc acagccgcct
    11221 gaatcgcaac gaatgcgaat tcggcatcac actggcggaa catatgcgcg gcagcggact
    11281 ggcacagaaa atgatggaac tcatcatcca aaccgccgca cagcaaggct accggactat
    11341 gagtgccgac attctcaaaa ccaatacccc catgatcaaa cttgctgaaa aatcaggatt
    11401 taccctcaag gaatcggaca ccgaaaaaaa cctgtaccgc gcatatctga accttgcggc
    11461 agacaaaaca acagaaaaaa caaataaaaa cttgcacacc gaccacaaaa taacctaaaa
    11521 tcggcagttt ccatatatcc gattttctca aaaggactca aaatggtagt tatccgtttg
    11581 gcacgcggcg gctcgaaaca ccgccctttc tacaacgtca tcgttactga ctcacgcagc
    11641 cgccgcgacg gccgcttcat cgaacgcgta ggcttctaca accccgtagc caacgaaaaa
    11701 caagagcgcg tccgcctcaa cgcagaccgc ctgaaccact ggattgcaca aggcgcgcaa
    11761 gtcagcgact ccgttgcaaa actgattaaa gaacaaaaag ccgcctaatc cacatttgcc
    11821 gccatgacag acactcaaaa ccgggtagcc atgggctaca tcaaaggcgt attcggcata
    11881 aaaggctggt tgaaaattgc cgccaacacc gaatattccg acagcctttt ggactacccc
    11941 gagtggcatt tggtcaagga cggcaaaacc atcagcgtta cccttgaagc cggaaaagtc
    12001 gtcaacagcg aactccaagt caaattcgaa ggcataaacg accgcgactt ggcattctca
    12061 ttgcacggtt acaccatcga aataccccgt gaagcattcg ccccgacaga agaagacgaa
    12121 tactactgga cagacttggt cggcatgacc gtcgtcaaca aagaccatac cgttttaggc
    12181 aaggtaagca acctgatgga aaccggcgca aacgacgtat tgatgattga cggagaacac
    12241 gggcagattc tgattccgtt cgtttcccaa tatatcgaaa ccgtcgatac cggcagcaag
    12301 attattactg ccgactgggg tttggactac tgatgcttat ccaggcagtt accattttcc
    12361 ccgaaatgtt cgacagcatt actcgctacg gcgtaacggg acgcgcgaac agacagggaa
    12421 tctggcagtt tgaagcagtc aatccccgaa agtttgccga caacagattg ggctacatcg
    12481 acgaccgccc gttcggcggc ggcccgggaa tgattatgat ggctccgccg cttcatgcag
    12541 caatagaaca cgccaaagca caatcttccc aagctgcaaa agtcatttac ctcagccccc
    12601 aaggaaaacc gctgacacac ccaaaagcgg cagaactggc agaacttccg catctgattc
    12661 tgctgtgcgg acgctatgag ggaatagacg aaagactgct gcaaaccagc gtcgatgaag
    12721 aaatcagcat cggagacttc gttgtttccg gcggagagct tcccgccatg atgctgatgg
    12781 atgcggtatt gaggctcgta cccggcgtat tgggcgatat gcagtctgcc gaacaagatt
    12841 cgttctcaag cggcattttg gactgccccc actacaccaa acccttagaa tttcaaggca
    12901 tggctgttcc ggaagtatta cgttccggca atcatggctt gatagcggaa tggcggttgg
    12961 aacaatcgct gcgccgcacc ttggagcgca gacccgatct tttggaaaag cgcagtttaa
    13021 tcccaaagga atcccgcatc ttgaataaaa tcctacaaga gcaacgggaa atccaatcat
    13081 aatttaggaa aaaaacaatg aacctgattc aacagctcga gcaagaagaa attgcccgcc
    13141 tgaacaaaga aatccccgaa ttcgcaccgg gcgacaccgt agtcgtatcc gtacgcgtcg
    13201 tggaaggtac ccgcagccgt ctgcaagcct acgaaggcgt tgtcattgca cgccgcaacc
    13261 gtggtctgaa cagcaacttc atcgtccgta aaatttccag cggcgaaggt gttgaacgta
    13321 ctttccaact gtactctccg accgtcgaaa aaatcgaagt caaacgccgt ggcgacgtac
    13381 gccgtgccaa actgtactac ctgcgcggtc tgaccggcaa agctgcacgc atcaaagaaa
    13441 aactgcctgc acgcaaaggt tgattcaaac ccgttttccc caatgccgtc tgaaccttct
    13501 gacggcattt cttattgccg caacatccgc aacggaacag caccaagaag cacacccctt
    13561 ccggcacatc ccgaatcccc gtccgaaccg aacaaaattt acagaccaaa cgttatacag
    13621 tatcatatca gccatataat aataaccgct ttaccgcttt ccctttcaaa accgcaaatc
    13681 atcatgacac cttccctttt actatcagga ttgaccttcc gcctcgtcct tgccctgatt
    13741 gccgtatccc ttttatgggg cgtttacttt tgggcagtat ccgcatgagc atcattgtcg
    13801 aaaacctgac cgtcagctac cgccgccgcc ccgccgtgca ccatgtggac attacttttg
    13861 aagaacatag tatgtgggcg gttttcggtc ccaacggcgc agggaaatct acctttctta
    13921 aatccttgat gggattgcag cccatcgaca ctggcagcat ccggctggac ggattgaccc
    13981 gtcagaacat tgcctacctt ccccagcaat ccgacatcga ccgctcgcag cctatgaccg
    14041 ttttcgactt ggcggcaatg gggctgtggt atgaaatcgg ttttttcaga ggaataaatg
    14101 ccgttcaaaa acaacgcgtt cacgaatctt tggaacgcgt cgggatgcgg cagtttgccg
    14161 gccgccaaat tgcccacctt tccaacggac aattccagcg tgtccttttt gcccgaatgc
    14221 tggttcaaaa cgccaaattc ctacttctgg acgaaccttt caacgctgtt gatgcacgga
    14281 caacctacga gcttctcgac gtgttgcaaa aatgccattg cggcggacac gctatcattg
    14341 ccgttttgca cgattacgaa caagtccgtg cctactttcc caataccctg ctgctcgccc
    14401 gagaaaaaat tgcggcaggc gcaaccgaga ccattctgac agaaccctac ctcgcccaag
    14461 ccaacgccaa aatgcagcaa caggaaagcc ccgagtggtg cgcgtcataa atgccgtctg
    14521 aaaccgaaaa accatgaacc tctacgacct gctccttgcc ccctttgcag aattcgactt
    14581 catgtgctac gccctcgcat ccgtcttctg cctgtccctc agtgccgcac ccgtcggcgt
    14641 attcctcgtc atgcgccgta tgagcctgat aggcgacgca ttgagccacg ccgtcttgcc
    14701 cggtgccgcc gtcggctaca tgtttgccgg cttgagcctg cccgccatgg gtttgggcgg
    14761 cgtagccgca ggcatgctga tggcactgct tgccggactt gttagccgct tcaccaccct
    14821 gaaagaagat gccaactttg ccgcctttta tctcagcagc ctcgccatcg gtgtagtcct
    14881 cgtcagcaaa aacggcagca gcgtcgattt gctgcatctg cttttcggct ctgtacttgc
    14941 cgtcgatatt cctgccctgc aactcatcgc cgccgtatcc accctcacac tgcttaccct
    15001 tgccgtcatc taccgcccgc tcgtactcga aagcatcgac cccctgttcc tcaaatccgt
    15061 cggcggcaaa ggcgggcttt ggcacgtcct ctttctcgtc ctggtcgtca tgaacctcgt
    15121 atccggcttt caagccctcg gaacactcat gtccgtcgga ctcatgatgc tgccagccat
    15181 taccgcccgc ctgtgggcga agcacatggg cgcactcatc ctcctatccg ttctgacagc
    15241 cctgctgtgc ggcttgagcg gactgcttat ttcctaccac atcgaaattc cctccggtcc
    15301 cgccatcatc ctctgttgca gcgtccttta tctcttttcc gtcatactcg gcaaagaagg
    15361 cggcattctg accaaatggc tcaaaaacca ccgccaccac accacctgac cccgcaagcc
    15421 gcttgaacaa acaggcagct tgttttacca taacagatgt tacgttatat cacaggagaa
    15481 cagttatgaa acacctcaaa ctcaccctta ttgccgcatt gctaaccacc gccgcaactg
    15541 ccgcccccct tccggttgta accagtttca gcattttggg cgacgtagcc aaacaaatcg
    15601 gcggagagcg cgtatccata caaaatttag tcggcgccaa ccaagatacg cacgcctatc
    15661 atatgaccag cggcgacatt aaaaaaatcc gcagtgcaaa actcgtcctg attaacggct
    15721 tgggacttga agctgccgat gtgcaacgcg ccgtcaaaca aagcaaagta tcctataccg
    15781 aagcgaccaa aggcatccaa cccctcaaag ccgaagaaga aggcggacac catcacgacc
    15841 acgatcatga ccacgaagga caccaccacg accacggcga atatgacccc cacgtctgga
    15901 acgaccccgt ccttatgtcc gcctatgccc aaaacgtcgc cgaagccctg ataaaggccg
    15961 accccgaagg caaagtttat tatcaacaac gcttgggcaa ctaccaaatg cagctcaaaa
    16021 aactgcacag cgacgcacaa gccgcattta acgccgtccc tgccgccaaa cgcaaagtcc
    16081 tgaccgggca cgatgccttt tcctatatgg gcaaacgtta ccatatcgaa ttcatcgccc
    16141 cgcaaggcgt gagcagcgaa gccgagcctt cagccaaaca agtcgccgcc atcatccgac
    16201 aaatcaaacg cgaaggcatc aaagccgtat ttaccgaaaa tatcaaagac acccgcatgg
    16261 ttgaccgcat cgccaaagaa accggcgtca acgtcagcgg caaactgtat tccgacgcac
    16321 tcggcaacgc gcccgcagac acctacatcg gcatgtaccg ccacaacgtc aaagccttga
    16381 ccaacgcgat gaagcaataa aaccgtttga ttggtttgaa tttgaaaagg caaaaggccg
    16441 tctgaaaatt atagtggatt aacaaaaatc aggacaaggc gacaaagccg cagacagtac
    16501 agatagtacg gtaaggcgag gcaacgctgt actggttttt gtgaatccac tatattgcta
    16561 gaagtgcacc gattaaggtt aattaaaat
//
LOCUS       DAWFLT010000037        16208 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWFLT010000037 DAWFLT010000000
VERSION     DAWFLT010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16208
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.037"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..69
                     /locus_tag="WGR74_001209"
     CDS             <1..69
                     /locus_tag="WGR74_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0905634.1"
                     /translation="LRFAIREGGRTVGAGVVSSVIA"
     gene            76..151
                     /locus_tag="WGR74_001210"
     tRNA            76..151
                     /locus_tag="WGR74_001210"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:109..111,aa:Trp,seq:cca)
     gene            266..544
                     /gene="secE"
                     /locus_tag="WGR74_001211"
     CDS             266..544
                     /gene="secE"
                     /locus_tag="WGR74_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ0905635.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWFEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            546..1082
                     /gene="nusG"
                     /locus_tag="WGR74_001212"
     CDS             546..1082
                     /gene="nusG"
                     /locus_tag="WGR74_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ0905636.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1183..1617
                     /gene="rplK"
                     /locus_tag="WGR74_001213"
     CDS             1183..1617
                     /gene="rplK"
                     /locus_tag="WGR74_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ0905637.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1617..2312
                     /gene="rplA"
                     /locus_tag="WGR74_001214"
     CDS             1617..2312
                     /gene="rplA"
                     /locus_tag="WGR74_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ0905638.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            2542..3042
                     /gene="rplJ"
                     /locus_tag="WGR74_001215"
     CDS             2542..3042
                     /gene="rplJ"
                     /locus_tag="WGR74_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ0905639.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3100..3471
                     /gene="rplL"
                     /locus_tag="WGR74_001216"
     CDS             3100..3471
                     /gene="rplL"
                     /locus_tag="WGR74_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ0905640.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            3662..7840
                     /gene="rpoB"
                     /locus_tag="WGR74_001217"
     CDS             3662..7840
                     /gene="rpoB"
                     /locus_tag="WGR74_001217"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ0905641.1"
                     /translation="MSYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVVRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNRLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            7992..12167
                     /gene="rpoC"
                     /locus_tag="WGR74_001218"
     CDS             7992..12167
                     /gene="rpoC"
                     /locus_tag="WGR74_001218"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ0905642.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            12458..12715
                     /locus_tag="WGR74_001219"
     CDS             12458..12715
                     /locus_tag="WGR74_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905643.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRMGMPINIG"
     gene            12712..12861
                     /locus_tag="WGR74_001220"
     CDS             12712..12861
                     /locus_tag="WGR74_001220"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0905644.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSSKEWFVS
                     IEKTY"
     gene            13040..13411
                     /gene="rpsL"
                     /locus_tag="WGR74_001221"
     CDS             13040..13411
                     /gene="rpsL"
                     /locus_tag="WGR74_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ0905645.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            13529..13999
                     /gene="rpsG"
                     /locus_tag="WGR74_001222"
     CDS             13529..13999
                     /gene="rpsG"
                     /locus_tag="WGR74_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ0905646.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            14018..16123
                     /gene="fusA"
                     /locus_tag="WGR74_001223"
     CDS             14018..16123
                     /gene="fusA"
                     /locus_tag="WGR74_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ0905647.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEVDKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
BASE COUNT         4298 a         3599 c         4204 g         4107 t
ORIGIN      
        1 ctgcgctttg cgattcgcga aggcggtcgt accgtgggtg ccggcgtggt ttcttctgtt
       61 atcgcttaag tttagaggcc aatagctcaa ttggtagagt atcggtctcc aaaaccgagg
      121 gttgggggtt cgagaccctc ttggcctgcc aaataaaaaa ttaaccggcc ttgtgtcggt
      181 taattttttt gtatttgtta tttagtaaac tctcttgcca tttacatgga ttgagaatag
      241 acagatgcta tgatggataa ataatatgac agaacatacg cctgaaaaaa agaacgttaa
      301 agtggatcaa ctggttgttc aagataaaga atctgcatct aattccggta aggaagggtt
      361 ttttgcatat ttctcaaatt cttggttcga attcaaaaag gtggtttggc ctaagcgtga
      421 agatgctgtc agaatgactg tatttgttat agtgtttgtt gctgtgcttt ctatatttat
      481 ctatgcggca gatacagcaa tttcgtggtt attttttgat gtattgctga gaagggaagg
      541 ttgagatgtc gaaaaaatgg tatgttgtac aggcgtattc ggggtttgag aagaatgtcc
      601 aacgaatatt ggaagagcgc attgcccgtg aggagatggg agattatttc ggacaaattc
      661 tggtgcctgt agagaaagtt gttgatatcc gcaatggtcg taagactatt agtgaaagaa
      721 agtcatatcc tggttatgtg ctagttgaga tggaaatgac agatgactct tggcatcttg
      781 taaaaagcac cccccgtgtt tccggtttta ttggagggag ggctaataga cctacgccga
      841 ttagtcagag agaggctgaa attattttac agcaggttca gaccggcata gagaagccga
      901 aaccaaaagt tgaatttgag gtcggtcaac aggttcgtgt aaatgaaggg ccgtttgcgg
      961 attttaacgg ggtggttgag gaggtcaatt atgaacggaa taagttacgc gtgtctgttc
     1021 agatatttgg tagagaaaca cccgttgagc tggagttcag ccaggttgaa aagattaact
     1081 gatttttata cttgaaaaaa aagcaataag aggatagaat caaaaattaa cttggggagc
     1141 ggaaatggtt ccgcgtctta cccgttttta ggagttcgtt aagtggcaaa gaaaattatc
     1201 ggctatatta aactgcaaat tcctgcaggt aaagccaatc catctcctcc ggttggtcct
     1261 gctttgggtc agcgcggttt gaatattatg gaattttgta aggcatttaa tgctgcaacc
     1321 caaggtatgg agcctggctt accgattccg gttgtgatta ctgcatttgc agataaatca
     1381 ttcacatttg tgatgaaaac cccgccagct tctatcttgt tgaaaaaggc tgccggtttg
     1441 caaaaaggta gttctaatcc tctgaccaac aaagtgggta aattgacccg tgcccagttg
     1501 gaagaaattg ctaaaactaa agagcctgat ttgactgctg ctgacttgga tgcggctgtc
     1561 cgtactatag caggttctgc tcgctcaatg ggcttggatg tggagggtgt tgtataatgg
     1621 ctaaagtatc taaacgcttg aaagctcttc gctcttctgt ggaagccaat aaattatatg
     1681 caattgatga agcaattgct ttggtaaaaa aagcagcgac tgctaaattt gacgagtctg
     1741 ttgacgtatc tttcaacttg ggcgttgatc cgcgtaaatc tgaccaagtt atccgtggtt
     1801 cagtcgttct gcctaaaggc accggtaaga taactcgcgt agctgtattt actcaaggtg
     1861 caaatgcaga agctgctaaa gaagctggtg cagatatcgt cggtttcgaa gatttggctg
     1921 ctgaaatcaa agcaggcaat ctgaactttg atgtcgttat tgcttctccc gatgcaatgc
     1981 gtattgttgg tcagttgggt actattttgg gtcctcgagg cttgatgcca aaccctaaag
     2041 taggtactgt tactcctaac gttgctgaag cagtaaaaaa tgcaaaagca ggtcaagtac
     2101 aataccgtac agataaagca ggtatcgttc atgcaacgat tggtcgtgct tctttcgctg
     2161 aagctgactt gaaagagaac tttgatgcgt tgctggatgc tatcgttaaa gccaagcctg
     2221 ctgccgctaa aggtcagtat ctgaaaaaag ttgctgtgtc tagcaccatg ggtttgggta
     2281 ttcgcgttga tacatcaagc gtaaataact aatcttaagg aattttcaag cagtttggtt
     2341 ttctgggctg cttgaatttg ggctacttaa aattaagtag atgtccaaga ccgtagggat
     2401 cgtaagattt aatcgtaact gccctacgca gacggtagtc ctgaaacaca ttgcaagatt
     2461 gcttgtaaga tgtcttttta ggttaccgcg ctggtgggat atcgttttgg tatcctgttt
     2521 ataaacagtg ggaggtagac cttgagtctc aatattgaaa ccaagaaagt ggcggtcgag
     2581 gaaattagcg cggcaattgc taatgctcaa accctcgtag tcgctgaata tcgcggtatc
     2641 agtgtttcca gtatgactga gcttcgtgcg aatgcacgta aagaaggcgt ttatttgcgc
     2701 gttctgaaaa atactttggc tcgtcgtgca gtgcaaggta cttcatttgc agaattggcc
     2761 gatcaaatgg ttggtccgtt ggtttacgct gcttctgaag atgctgttgc tgctgctaaa
     2821 gtgttgcacc aattcgcgaa aaaagatgac aaaattgtcg ttaaagccgg ttcttacaat
     2881 ggcgaagtaa tgaatgctgc tcaggttgct gagttggctt ctattccgag ccgcgaagag
     2941 ctgttgtcca aactgttgtt cgttatgcaa gctcctgtat cgggctttgc gcgcggtttg
     3001 gctgctttgg cagagaaaaa agccggcgaa gaagccgctt aatcgatttt gtttctgtta
     3061 atcaattatt ttttaataca atatttggag taaaatagca tggctattac taaagaagac
     3121 attttggaag cagttggttc tttgaccgta atggaattga acgacttggt taaagctttt
     3181 gaagaaaaat tcggtgtttc tgctgctgct gttgcagttg caggtcctgc tggtgccggt
     3241 gctgccgatg ctgaagaaaa aaccgaattt gatgtcgttt tggcttctgc cggtgatcaa
     3301 aaagtcggcg tgattaaagt tgtccgtgca attaccggtt tgggtctgaa agaagctaaa
     3361 gacatcgttg acggtgcacc taaaaccatt aaagagggtg tttctaaagc tgaagccgaa
     3421 gacatccaaa aacaactgga agaagccggc gctaaagtcg aaatcaaata atttgatgct
     3481 tcttatgaag gctggcagtt ttctgccagc cttattttgc ttcttaaaat aagcattaag
     3541 tattgtttac atttatttgc atagttttta tcaaatcatt gcaaataaat gtaaacatca
     3601 gattgatgcg tgccgttgct ttcagacggc ctattattga aaattacttt tcggagtgta
     3661 tatgagctat tcgtttaccg agaaaaaacg tatccgtaag agttttgcaa agcgggaaaa
     3721 tgttttggaa gttcctttct tgctggcaac ccaaattgat tcttatgcga agtttttgca
     3781 gctggaaaat gcttttgaca aacgtaccga tgacggtctg caggcggcat ttaattctat
     3841 tttcccgatt gtgagccata acggttatgc gcgattggag tttgtgcatt acacattggg
     3901 cgagcctttg ttcgatattc ccgaatgtca gttgcgcgga atcacttatg cagccccctt
     3961 gcgcgcgcgt atccgtttgg tgattttgga taaggaagca tctaaaccga cggtaaaaga
     4021 agttcgtgaa aacgaagtgt atatgggcga aattccgttg atgaccccga gcggttcttt
     4081 tgtgattaac ggcacagagc gtgtgattgt ttcccagttg caccgttcgc ccggcgtgtt
     4141 tttcgagcat gacaaaggta agacgcactc ttccggcaaa ttgttattct ccgcccgcat
     4201 cattccctac cgtggttcat ggttggattt tgaatttgat ccgaaagatt tgctgtattt
     4261 ccgtatcgac cgccgccgta aaatgccggt aacgattttg ttgaaggctt tgggctacaa
     4321 caatgagcaa atcttggata ttttctacga caaagaaacg ttctatttgt cttcaaacgg
     4381 tgttcaaacc gatttggtcg caggccgtct gaaaggcgaa actgccaagg tcgatatctt
     4441 ggataaagaa ggcaatgtat tggttgccaa aggtaagcgc attactgcga aaaatatccg
     4501 tgatattacc aatgcaggcc tgacccgttt ggatgtagaa ccagaaagcc tgctgggcaa
     4561 agcattggct gccgacctga ttgattcgga aaccggcgag gtattggctt ctgccaatga
     4621 tgaaattaca gaagagttgt tggccaaatt tgatatcaac ggcgtaaaag aaattacgac
     4681 cctttacatc aatgagttgg atcagggtgc ttatatctcc aataccttgc gtacggatga
     4741 gactgccggc cggcaggcgg cgcgtgttgc gatttaccgt atgatgcgtc cgggcgaacc
     4801 gcccactgaa gaggcggtcg agcaattgtt taaccgcttg ttcttcagtg aagacagcta
     4861 cgatttgtcc cgcgtaggcc gtatgaaatt taatacgcgc acatacgaac aaaaactgtc
     4921 cgaagcccaa caaaactctt ggtacggccg cctgctgaac gaaacgtttg ccggtgctgc
     4981 cgacaaaggc ggttatgtcc tgagcgtcga agatattgtc gcctcgattg cgactttggt
     5041 cgagttgcgt aacggccatg gcgaagtgga cgatatcgat cacttgggca accgccgagt
     5101 acgttcggta ggcgagctga ctgaaaacca attccgtagc ggtttggccc gtgtggaacg
     5161 tgccgtaaaa gaacgtttga atcaggcgga atcagaaaac ttgatgccgc acgatttgat
     5221 taatgcaaaa cctgtttctg ctgctattaa agaattcttc ggctccagcc aattgagtca
     5281 gtttatggat cagaccaatc ccttgtctga agtaacccat aaacgccgcg tatccgcatt
     5341 gggtccgggc ggtttgaccc gcgaacgtgc aggatttgag gtgcgggacg tgcatccgac
     5401 ccactacggc cgcgtatgtc cgattgaaac gcctgaaggt ccgaacatcg gtttgatcaa
     5461 ctcactgtcc gtttatgcgc gcaccaatga ttacggtttc ttggaaacgc cttaccgccg
     5521 cgttatcgac ggcaaagtaa ccgaggaaat cgattacttg tctgccatcg aagaaggccg
     5581 ctatgtgatt gcacaggcga atgccgattt ggattcagat ggcaatctga ttggcgattt
     5641 ggttacctgt cgtgaaaaag gcgaaaccat tatggcaacg cccgaccgcg tccaatatat
     5701 ggacgtggca accggccagg tggtatccgt tgcggcatcc ctgattccgt tcttggaaca
     5761 tgatgacgcg aaccgcgcat tgatgggtgc caacatgcaa cgtcaggcag tgccttgctt
     5821 gcgtcctgaa aaaccgatgg tcggtaccgg tatcgagcgt tccgttgccg ttgactctgc
     5881 tactgcaatc gttgcccgcc gaggcggcgt ggtcgagtat gtcgatgcca accgcgtcgt
     5941 cgttcgtgtt catgacgatg aagcgactgc cggcgaagtg ggtgtcgata tttacaactt
     6001 ggttaaattt acccgttcca atcaatctac taacatcaac caacgtccgg ctgtcaaagc
     6061 aggcgatgtt ttgcaacgcg gcgatttggt ggccgatggc gcgtccaccg atttgggcga
     6121 attggctttg ggtcaaaata tgaccatcgc cttcatgccg tggaacggtt acaactacga
     6181 agactcgatt ctgatttccg aaaaagtggc tgcggacgac cgctatactt cgattcacat
     6241 tgaggaattg aatgtcgttg cccgcgatac caagctgggt gcggaagaca ttacccgcga
     6301 tattccgaac ttgtccgagc gtatgcaaaa ccgtttggac gaatccggta tcgtttacat
     6361 cggtgcggaa gtagaagccg gcgatgtgtt ggtaggcaag gtaacgccta aaggcgaaac
     6421 ccagctgacg ccggaagaaa aactgctgcg cgccatcttc ggtgaaaaag catctgacgt
     6481 aaaagatact tcattgcgta tgcctaccgg catgagcggt accgttatcg acgttcaagt
     6541 cttcactcgt gaaggtattc aacgcgacaa acgtgctcaa tccattatcg attccgagtt
     6601 gaaacgctac cgtttggatt tgaacgacca attgcgtatt ttcgacaacg acgcattcga
     6661 ccgtatcgag cgtatgattg tcggtcagaa agccaacggc ggcccgatga agctggccaa
     6721 aggcagtgaa atcacgaccg aatatctggc gggtctgccg agcaggcacg attggttcga
     6781 tatccgtctg accgatgaag atttggccaa gcagttggaa ctgattaaag tgagcctgca
     6841 acaaaaacgc gaagaagcgg acgagttata cgaaatcaag aagaaaaaac tgacccaagg
     6901 cgacgaattg caacccggcg tacaaaaaat ggtgaaagtt tttatcgcca tcaaacgccg
     6961 tctgcaagcc ggcgacaaaa tggcgggccg ccacggtaac aaaggcgtgg tatcgcgcat
     7021 tctgccagtg gaagacatgc cttacatggc ggacggccgt ccggtagaca tcgtactgaa
     7081 cccattgggc gtaccttccc gtatgaacat cggtcagatt ttggaagttc acttgggttg
     7141 ggcagcaaaa ggtatcggcg agcgtatcga ccgtatgctg aaagagcaac gcaaagcagg
     7201 cgagttgcgc gagttcttga acagactcta caacggcagc ggtaagaaag aagatttgga
     7261 tgccctgact gatgaagaaa tcatcgaact ggcctccaac ctgcgcaaag gtgcatcttt
     7321 cgcctctcct gtattcgacg gtgcgaaaga gtctgaaatc cgcgaaatgc tgaacttggc
     7381 ttatccgagc gacgatcctg aggttgaaaa actgggcttc aacgacagta aaacccaaat
     7441 cacgctgtat gacggccgtt caggcgaagc atttgaccgc aaggttacag taggtgtgat
     7501 gcactatctg aaactgcacc acttggttga cgaaaaaatg cacgcccgtt ctaccggtcc
     7561 gtacagtctg gttacccagc agcctttggg cggtaaagcc cagttcggcg gccaacgttt
     7621 cggcgagatg gaggtttggg cattggaagc atacggcgcg gcatacacgc tgcaagagat
     7681 gttgactgtg aagtctgacg acgtgaacgg tcgtaccaaa atgtacgaaa acatcgtcaa
     7741 aggcgaacac aaaatcgatg ccggtatgcc cgagtccttc aacgtattgg tcaaagagat
     7801 tcgctcactg ggcttggata tcgatttgga acgctactga tacgggtttc agacggcata
     7861 aggggagctg ttctgcaggt atgcggggca gccgacaatg tttaaaaacg aaatgccgtc
     7921 tgaaaacact gtacctctat ccatatcgaa aatccgccat gcggtaaaaa tacttccttc
     7981 aaggagcaaa aatgaatttg ttgaacttat ttaatccgtt gcaaactgcc ggcatggaag
     8041 aagagtttga tgccatcaaa atcggtattg cctctcccga aaccatccgc tcatggtctt
     8101 atggcgaagt caaaaaacct gaaaccatca actaccgtac gttcaaacct gagcgtgacg
     8161 gtttgttctg tgccaaaatc tttggcccgg tcaaagacta cgaatgcttg tgcggaaaat
     8221 acaaacgctt gaaatttaaa ggtgtaactt gtgaaaaatg cggcgtggaa gtaaccctgt
     8281 ccaaagtgcg ccgcgaacgc atgggccata tcgaattggc tgcgcccgtc gcacatattt
     8341 ggttcttaaa atccctgcct tcccgcttgg gtatggtgtt agacatgact ttgcgcgata
     8401 tcgaacgcgt attgtacttt gaagcatttg tggtaaccga tcccggtatg actccgctgc
     8461 aacgccgcca attgctgact gaagacgatt actacaacaa gctggacgaa tacggcgacg
     8521 atttcgatgc caaaatgggt gcggaaggta tccgcgaatt gctgcgtacc ctgaatgtag
     8581 cgggcgaaat cgaaatcctg cgccaagagt tggaatcgac cggttccgac accaaaatca
     8641 aaaaaatcgc caaacgcttg aaagtattgg aagctttcca tcgttccggt atgaaactgg
     8701 aatggatgat tatggatgtg ctgccggtat tgccgcctga tttgcgtccg ttggttccat
     8761 tggatggtgg tcgttttgcc acttccgatt tgaacgattt gtaccgccgc gttattaacc
     8821 gtaacaaccg tctgaaacgt ctgttggaac tgcatgctcc tgacatcatc gtccgcaacg
     8881 aaaaacgtat gttgcaagaa gcagttgact cgctgttgga taacggccgt cgcggtaaag
     8941 ccatgaccgg cgccaacaaa cgcccgctga aatcattggc agacatgatt aaaggtaagg
     9001 gtggccgctt ccgtcaaaac ctgctgggca aacgtgtgga ctactccggc cgttccgtga
     9061 ttaccgtagg cccgtacctg cgtctgcacc aatgcggttt gccgaaaaaa atggctttgg
     9121 aactgttcaa accgttcatt ttccacaaat tggaaaaaca aggtttggcc tctaccgtta
     9181 aagcagcgaa aaaattggta gagcaagaag taccggaagt atgggacatc ttggaagaag
     9241 tcatccgcga acatccgatt atgctgaacc gtgcgccgac cctgcaccgt ttgggtattc
     9301 aagcgttcga acctatcttg attgaaggta aagcgattca gttgcaccca ttggtgtgtg
     9361 ctgcgttcaa cgccgacttt gacggcgacc aaatggcggt acacgttcca ttgagcttgg
     9421 aagcacaaat ggaagcacgc acgctgatgc tggcttcaaa caacgtattg tctcctgcca
     9481 acggcgaacc gattatcgta ccttcccaag acatcgtatt gggcctgtac tacatgaccc
     9541 gcgaccgtat caatgccaaa ggcgagggca gcctgtttgc cgatgtgaaa gaagtgcatc
     9601 gcgcatacca taccaaacag gttgaactgg gtacgaaaat taccgtacgt ctgcgcgaat
     9661 gggtgaaaaa cgaagcaggt gagtttgagc ctgtcgttaa ccgttacgaa acaaccgtcg
     9721 gccgtgcatt gttgagcgaa atcctgccga aaggcctgcc gtttgaatat gtcaacaaag
     9781 cgttgaagaa aaaagaaatt tctaaactga ttaacgcatc gttccgcctg tgcggcttgc
     9841 gcgatacggt tatctttgct gaccacctga tgtacaccgg tttcggattt gcggcaaaag
     9901 gcggtatttc cattgccgtt gacgatatgg aaattccaaa agaaaaagcg gccttgctgg
     9961 ctgaagccaa tgccgaggtt aaagaaatcg aagaccaata ccgtcaaggt ttggttacca
    10021 acggcgaacg ctacaacaag gtggtcgata tttggggtcg tgccggcgat aagattgcta
    10081 aagcgatgat ggacaacttg tccaaacaaa aagttatcga ccgtgacggc aacgaagtcg
    10141 atcaagagtc attcaactcc atttatatga tggcggactc cggtgcccgt ggttctgcag
    10201 ctcagattaa acagttgtcc ggtatgcgtg gcttgatggc aaaacctgac ggctcgatta
    10261 ttgaaacgcc gattacctca aacttccgtg aaggtctgac cgtattgcaa tactttattg
    10321 cgacccacgg tgcgcgtaag ggtttggcgg ataccgcatt gaaaaccgcg aactccggtt
    10381 acctgactcg tcgtctggta gacgtaactc aagatttggt cgttgttgaa gacgattgcg
    10441 gtacttcaga cggctttgtc atgaaggcag tggtacaagg cggtgatgtg attgaagcat
    10501 tgcgcgatcg tattttgggt cgtgttaccg cgtctgacgt tgtcgatccg tcaagtggcg
    10561 aaaccttggt tgaagccggt acgttgctga ctgaaaaact ggtggatatg atcgaccaat
    10621 ccggtgtcga tgaagtcaaa gtccgtacgc cgattacttg taaaacccgt cacggcctgt
    10681 gtgcacactg ttacggtcgt gacttggcac gcggcaaact ggttaacgcc ggtgaggcag
    10741 tcggtgtgat tgctgcacaa tccattggcg aaccgggtac ccagttgacc atgcgtacgt
    10801 tccacatcgg tggtgcggca tcccgtgcgg cagcagccag ccaagtggaa gccaaatcca
    10861 acggtacggc acgattcagc agccagatgc gctacgttgc caacaacaaa ggcgagttgg
    10921 ttgtcatcgg ccgttcttgt gaagtcgtga ttcacgacga tatcggccgt gaacgcgaac
    10981 gccacaaagt accttacggt gccatcctgc tggtacaaga cggtatggcc attaaagccg
    11041 gtcaaacctt ggcaacctgg gatccgcata cccgtccgat gattaccgaa cacgcaggta
    11101 tggtgaaatt cgaaaacgtg gaagagggcg ttaccgttgc caaacaaacc gatgatgtaa
    11161 ccggtttgtc cactttggtg gtgattgacg gtaaacgtcg ttcctctagt gcttccaaac
    11221 tgctgcgtcc gactgtgaaa ctcttggacg aaaacggcgt ggaaatctgt attcccggta
    11281 cgtccactcc ggtttcgatg gcattccccg ttggtgcggt gattaccgta cgcgaaggtc
    11341 aggaaatcgg taaaggcgac gtattggcgc gtatcccgca agcctcttcc aaaacccgcg
    11401 acattaccgg tggtctgccg cgcgttgccg aattgtttga agcacgcgtg ccgaaagatg
    11461 ccggtatgtt ggcggaaatt accggtaccg tttccttcgg caaagagacc aaaggcaagc
    11521 aacgtctgat tgttactgac gtggacggtg tagcatacga gaccttgatt tccaaagaga
    11581 aacaaattct ggtacacgac ggtcaagtgg taaaccgcgg tgaaaccatc gtggacggag
    11641 ctgttgatcc gcatgatatt ctgcgtttgc aaggtatcga agcactggca cgctacattg
    11701 tccaagaggt gcaagaggtt taccgtctgc aaggtgtgaa gatttctgat aaacacatcg
    11761 aagtcatcat ccgtcaaatg ttgcgccgtg tgaacattgc ggatgccggc gaaaccgggt
    11821 tcattaccgg agagcaggtc gaacgcggcg atgtgatggc ggccaatgaa aaagctttgg
    11881 aagaaggcaa agagccggca cgttacgaaa acgtattgct gggtattacc aaagcctccc
    11941 tgtccaccga cagtttcatt tctgccgcat cgttccaaga aacgacccgt gttctgaccg
    12001 aagccgcgat tatgggcaaa caagacgaac tgcgcggttt aaaagagaac gtcatcgtcg
    12061 gccgcctgat tcctgccggt accggtttga cttatcaccg cagccgtcat cagcaatggc
    12121 aaggggtaga gcaggagact gctgaaaccc aagtaacgga tgaataatct tcggcgcgtt
    12181 tattcaatac aaaaccgcaa gctttaagct tgcggttttt ctttgtccga ttaaggcaaa
    12241 aacaagcgtt ttcgtcattt tgaggcgtgt ggattattcc ttaggtattt tcgggccgga
    12301 gaccaacgag gtggcgggtg tcgtcggtac gtccggagac caaaataact ttgccaagga
    12361 tgttggtttc ggcggtcaaa aaagtagcgt cttaatgttt tccatttaaa caaatgtcgt
    12421 ctgaaacttc agacggcatt tcctttaaaa aataaatatg aaacccagaa atctcttttt
    12481 tgcaggctgc ctgctgactt cggcgacgtt tgccgaggat atcggcgtac ctgtcgaact
    12541 gattaacgtc ggtaatcgga ttgcgatgcc gtctgaaggg gaaagcctcg ccctcctgcc
    12601 gtttgccgag gatgtaccgc cggttcgcga tgcaatgccg tctgaagttc ctaaaagcgc
    12661 ggcaggcggc gatgttcggg gtgaccggat gggaatgccg attaacatcg gatgagcgcg
    12721 gctttatggc ataaaaaact gtcgtggaaa ggatttacac cccaaataaa tttccgttac
    12781 aacaagatca acagcaatat gcccgccttt tattcgcgca gcagcaagga atggtttgtc
    12841 agtatagaaa aaacgtattg acagtatttt ctccagccgt ccgactgatt gtgagggatg
    12901 tcggtaaata tttatcggca aacaagaaaa tcatctttct tcttgtcgtt atgcttgact
    12961 gtatacttgc aataaaaata taattccact cttgccgaca tggtgtcggc aagtatttaa
    13021 ctcaacagga cgagaaaata tgccaactat caaccaattg gtacgcaaag gccgtcaaaa
    13081 gcccgtgtac gtaaacaaag tgcccgcact ggaagcctgc ccgcaaaaac gcggcgtgtg
    13141 cacccgtgta tacacgacta cccctaaaaa acctaactct gcattgcgta aagtatgtaa
    13201 agtccgcctg accaacggtt ttgaagtcat ttcatacatc ggcggcgaag gccacaacct
    13261 gcaagagcac agtgtcgtat tgattcgcgg cggtcgtgta aaagacttgc caggtgtgcg
    13321 ttaccacact gtacgcggtt ctttggatac tgcaggtgtt aaagaccgta aacaagcccg
    13381 ttccaaatac ggtgctaagc gtcctaaata attactggga cttaaatagg cacgtcggcc
    13441 gcctaagctg aacaacggcc gagtaagtga atactcaatt gggtattcat gggaatagac
    13501 ccgactgaat agattaaagg aaattaaaat gccaagacgt agagaagtcc ccaagcgcga
    13561 cgtactgcca gatcctaaat tcggcagcgt cgagttgacc aaattcatga acgtattgat
    13621 gattgacggt aaaaaatccg ttgccgagcg tatcgtttac ggtgcgttgg aacagattga
    13681 gaaaaaaacc ggcaaagtag caatcgaagt atttaacgaa gccattgcaa acgccaaacc
    13741 tatcgtggaa gtgaaaagcc gccgtgtagg tggtgcaaac taccaagttc ctgttgaagt
    13801 tcgtccttca cgccgtttgg ctttggcaat gcgctgggtt cgcgatgcgg cccgcaaacg
    13861 tggtgagaaa tccatggacc tgcgtttggc aggcgaattg attgatgcgt ccgaaggccg
    13921 tggcggtgcg ttgaaaaaac gtgaagaagt acaccgtatg gctgaagcca acaaagcatt
    13981 ctctcacttc cgtttctaat tttgaaaggc taataaaatg gctcgtaaga ccccgatcag
    14041 cctgtaccgt aacatcggta tttccgccca tatcgatgcg ggtaaaacca cgacgacaga
    14101 acgtattttg ttctataccg gtttgaccca caagctgggc gaagtgcatg acggtgcggc
    14161 tactaccgac tacatggaac aagagcaaga gcgtggtatt accattacct ctgctgccgt
    14221 tacttcctac tggtccggta tggcgaaaca attccccgag caccgcttca acatcatcga
    14281 caccccggga cacgttgact ttaccgtaga ggtagagcgt tctatgcgtg tattggacgg
    14341 cgcggtaatg gtttactgcg cggtgggcgg tgttcaaccc caatctgaaa ccgtatggcg
    14401 gcaagccaac aaataccaag tgccgcgctt ggcgtttgtc aataaaatgg accgtcaggg
    14461 tgccaacttc ttccgcgttg tcgagcaaat gaaaacccgt ttgcgcgcaa accctgtacc
    14521 tatcgtcatt ccggttggtg cggaagacaa cttcagcggt gtggttgatt tgttgaaaat
    14581 gaaatccatc atttggaatg aagtcgataa aggtacaacc tttacctatg gcgatattcc
    14641 tgccgaattg gtcgaaactg ccgaagaatg gcgtcaaaat atgattgaag ccgcagccga
    14701 agccagcgaa gaactgatgg acaaatactt aggcggcgac gagctgaccg aagaagaaat
    14761 cgtaggcgcg ttgcgtcaac gtactttggc aggcgaaatt cagcctatgc tgtgtggttc
    14821 tgcatttaaa aacaaaggtg ttcaacgtat gttggacgca gttgtagaat tgctgccagc
    14881 tcctaccgat attcctccgg ttcaaggtgt caacccgaat accgaggaag ccgacagccg
    14941 tcaagccagc gatgaagaga aattctctgc attggcgttc aaaatgttga acgacaaata
    15001 cgtcggtcag ctgaccttta tccgcgttta ctcaggcgta gtaaaatccg gcgataccgt
    15061 attgaactcc gtaaaaggca ctcgcgaacg tatcggtcgt ttggtacaaa tgactgccgc
    15121 agaccgtact gaaatcgaag aagtacgcgc cggcgacatc gcagccgcta ttggtctgaa
    15181 agacgttact accggtgaaa ccttgtgtgc ggaaagcgcg ccgattatct tggaacgtat
    15241 ggaattcccc gagccggtaa tccatattgc cgttgagccg aaaaccaaag ccgaccaaga
    15301 gaaaatgggt atcgccctga accgcttggc taaagaagac ccttctttcc gtgtccgtac
    15361 agacgaagaa tccggtcaaa ccattatttc cggtatgggt gagctgcact tggaaattat
    15421 tgttgaccgt atgaaacgcg aattcggtgt ggaagcaaat atcggtgcac ctcaagtggc
    15481 ttaccgtgaa actatccgca aagccgttaa agctgaatac aaacatgcaa aacaatccgg
    15541 tggtaaaggt caatacggtc acgttgtgat tgaaatggaa cctatggaac cgggtggtga
    15601 aggttacgag tttatcgatg aaattaaagg tggtgtgatt cctcgcgaat ttattccgtc
    15661 tgtcgataaa ggtatccgcg atacgttgcc taacggtatc gttgccggct atcctgtagt
    15721 tgacgtacgt atccgtctgg tattcggttc ttaccatgat gtcgactctt cccaattggc
    15781 atttgaattg gctgcttctc aagcgtttaa agaaggtatg cgtcaagcat ctcctgccct
    15841 gcttgagcca atcatggcag ttgaagtgga aaccccggaa gaatacatgg gcgacgtaat
    15901 gggcgacttg aaccgccgtc gcggtgttgt attgggtatg gatgatgacg gtatcggcgg
    15961 taaaaaagtc cgtgccgaag tacctctggc agaaatgttc ggttattcga ccgacctgcg
    16021 ttctgcaacc caaggccgcg ctacttactc tatggagttc aagaaatatt ctgaagctcc
    16081 tgcccacata gctgctgctg taactgaagc ccgtaaaggc taatcaggca aataggccgt
    16141 ccgaaaggct gaaatgattt ttcagacggc attgttcttt aatcgatctt taatgtaaag
    16201 gaattagc
//
LOCUS       DAWFLT010000038        15991 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWFLT010000038 DAWFLT010000000
VERSION     DAWFLT010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15991
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.038"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            275..1300
                     /locus_tag="WGR74_001224"
     CDS             275..1300
                     /locus_tag="WGR74_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein"
                     /protein_id="HEZ0905648.1"
                     /translation="MKYKALSLLPLVAALAACAGGGVAEPHVPVSIPTATPLPKGEVT
                     LSSDNGKIENINTTSTGSTSVISIQERAVTKNYFGVESQEKSFIFKTPGGAQYTLSSY
                     ADPITVSYSSPDFKIPDHHAGQRLADGSRIFICCSDSGATSYAEITKQDYMKFGAWIG
                     PNGEIDLFAGGFPVGKTPPPAFSYGSSTPETKGTGKITYQVWGIRVRNGQFVTSSYTP
                     PKNSGYTFNPTNTPVLSFITANFNSNKLAGKIIGNSDYGPDVEIKEAQIDGLSFSGDA
                     TSGGKTGKLEGKFFGKFNSSYDSDTSIGGKITFDGDRSLDTVFGGVSYKKELESTTDR
                     ETTHLTK"
     gene            1331..3763
                     /gene="hupB"
                     /locus_tag="WGR74_001225"
     CDS             1331..3763
                     /gene="hupB"
                     /locus_tag="WGR74_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HEZ0905649.1"
                     /translation="MHIPFKPVLAAAAIAQAFPAFAADPAPQSTQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGNVEIKNKGYVYDPNKPFNPSLGPSSYVATGVARSQPDPQEWVNKSTLFK
                     LGYNFNDRNRIGWIFEDSRTDRFTNELSNLWTSLTGDITGDYRHRQDVSYRRRSGVEY
                     KNELEHGPWDSLKLRYDKQRIDMNTWTWDIPKDYDTNGINGQVYHSFRHIRQNTAQWT
                     ADFEKQLDFSKAVWAAQYGLGGGKGDNANSDYSYFAKLYDPKILTTNQGKITMLIENR
                     SKYKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAH
                     AGFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPNLKAEKAK
                     NWELGLAGSGKAGNFKLSGFKTKYRDFIELTYMGVSSDNPNKPTYAPLSDGTALVSSP
                     VWQNQNRTAAWVKGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGRATQNNGKETPINAL
                     SPWTAVYSLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLF
                     DLSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSP
                     GRSYNFTIEAKF"
     gene            complement(4370..6004)
                     /gene="groL"
                     /locus_tag="WGR74_001226"
     CDS             complement(4370..6004)
                     /gene="groL"
                     /locus_tag="WGR74_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ0905650.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAMPDMGGMGGMGGMM"
     gene            complement(6097..6387)
                     /locus_tag="WGR74_001227"
     CDS             complement(6097..6387)
                     /locus_tag="WGR74_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ0905651.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
     gene            6616..7269
                     /locus_tag="WGR74_001228"
     CDS             6616..7269
                     /locus_tag="WGR74_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0905652.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEDPVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKLDVSGFIHYRINHSKEFADRQ
                     NHINGIENFWNQAKRVLRKYNGIDCKSFPLFLKECEFRFNFGTPSQQLKILRDWCGI"
     gene            7482..9017
                     /locus_tag="WGR74_001229"
     CDS             7482..9017
                     /locus_tag="WGR74_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0905653.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            9014..9106
                     /locus_tag="WGR74_001230"
     CDS             9014..9106
                     /locus_tag="WGR74_001230"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ0905654.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(9166..10386)
                     /gene="lysA"
                     /locus_tag="WGR74_001231"
     CDS             complement(9166..10386)
                     /gene="lysA"
                     /locus_tag="WGR74_001231"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ0905655.1"
                     /translation="MPLFCEQVPYPRLAEALGTPLYVYSQSALTEAFEHYQTAFAALS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IKFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLIEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQDENVPDLGAYARAVQKLMGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVEPKNIAPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGNEYRLIRRR
                     ETLEQQMANELACL"
     gene            complement(10397..10567)
                     /locus_tag="WGR74_001232"
     CDS             complement(10397..10567)
                     /locus_tag="WGR74_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0905656.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QSTPQSAPPTQK"
     gene            10638..10961
                     /gene="cyaY"
                     /locus_tag="WGR74_001233"
     CDS             10638..10961
                     /gene="cyaY"
                     /locus_tag="WGR74_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ0905657.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAEN
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDNRDFYDVLNEALSAASGEAV
                     KIAEL"
     gene            10988..12004
                     /locus_tag="WGR74_001234"
     CDS             10988..12004
                     /locus_tag="WGR74_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEZ0905658.1"
                     /translation="MNTRPFYFGLIFIAIIAIFANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGTLLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTVLLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAESVTKAESHKVSVAIAIV
                     VIFGTLAIFTYPLFYTWSQDLINAHQFGIYVGSSVHEVAQVYAIGENIDPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSDGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTHASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            12030..12449
                     /locus_tag="WGR74_001235"
     CDS             12030..12449
                     /locus_tag="WGR74_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ0905659.1"
                     /translation="MAQLPLYLTSEIKDFTVGTPKVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALSINDYIHAVYDFVEQ
                     AGYCRNGFPETGGEWVKVENRVLDDELLDKILSRKST"
     gene            12471..12977
                     /gene="luxS"
                     /locus_tag="WGR74_001236"
     CDS             12471..12977
                     /gene="luxS"
                     /locus_tag="WGR74_001236"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ0905660.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCVPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            13123..15918
                     /gene="polA"
                     /locus_tag="WGR74_001237"
     CDS             13123..15918
                     /gene="polA"
                     /locus_tag="WGR74_001237"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ0905661.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVIEHAS
                     EIKGKVGENLRAALPRLPLSYDLVTIKTDVDLHAELSDGLESLRRTAPKWAQLVVDFK
                     RWGFRTWLKEAESRMHEAADGDLFGSDTIGEQAALDMETSSERLPEKAVAPEKLDYQA
                     VTTEAQFATLLDKLSQAEKIGIDTETTSLDAMNAALVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGHLKPYLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPDMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPPGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSPEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGFVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSRWLVSDDLKSKLIMQVHDELVLEVVETELDFVKEKLPQIMAKVGGGLLD
                     VPLVAEVGVGENWEEAH"
BASE COUNT         3838 a         4337 c         4073 g         3743 t
ORIGIN      
        1 ctgcagatgc ccaacggtct ttatagtgga ttaacaaaaa tcaggacaag gcgacggagc
       61 cgcagacagt acaaatagta cggaaccgat tcacttggtg cttgagcacc ttagagaatc
      121 gttctctttg agctaaggcg aggcaacgcc gtactggttt ttgttaatcc actataccga
      181 tttttgcacc gacccacaaa accagttgac atcatataac atttaaactg ataatcaaaa
      241 ctattattaa cacatcatca agattgagta tgcgatgaaa tacaaagccc tgtccttact
      301 gccgcttgtc gctgcccttg ccgcctgtgc cggggggggg gtagccgaac cgcacgtccc
      361 cgtgtccatc cccaccgcca cgccgctgcc caaaggcgag gtaacgttat caagcgataa
      421 cggcaaaatc gaaaacatca acaccaccag caccggaagc acatccgtca tttccattca
      481 agagcgggcg gttacaaaaa actatttcgg tgttgaatca caagaaaaat cattcatctt
      541 caaaacaccc ggcggcgcgc aatacaccct ttcatcctac gccgacccca taaccgtcag
      601 ctactcctcc cccgatttca aaattcccga ccaccacgca gggcagcggc ttgccgacgg
      661 cagccgcatc tttatctgct gcagcgactc cggggctact tcgtatgcgg aaattaccaa
      721 acaagattat atgaaattcg gggcgtggat aggtcccaac ggcgaaatcg acctcttcgc
      781 cggcggcttc cccgtcggca aaacgccacc tcctgcgttc tcctacggca gcagcacacc
      841 cgaaaccaaa ggcacaggca aaatcactta tcaggtttgg ggcatccgcg tcagaaacgg
      901 gcaatttgtt acttcctctt atacgccgcc gaaaaacagt ggttatacgt tcaatcctac
      961 caacaccccc gtcctttcct ttatcaccgc caatttcaac agtaacaaac tggcaggcaa
     1021 aatcatcggc aacagcgatt acgggccgga tgtggaaatt aaagaagcac agattgacgg
     1081 attatccttt tccggcgatg ccacctccgg cggcaaaacc ggcaaattgg aaggcaagtt
     1141 ttttggcaaa ttcaacagca gctatgacag cgacaccagc ataggcggca aaatcacctt
     1201 tgacggcgac cgctccctcg ataccgtatt cggcggcgtg agttataaaa aagaacttga
     1261 aagcaccacc gatcgagaga ccacccatct cacaaaataa taattaatcc gcttttaaaa
     1321 aggttttctt atgcacatcc ccttcaagcc cgtattggct gccgccgcca tcgcccaagc
     1381 gtttcccgcc tttgcggcag accccgcgcc gcagtccacc caaacgctga acgaaatcac
     1441 cgttaccggc acgcacaaaa cccaaaaact cggcgaagaa aaaatccgcc gcaaaacctt
     1501 agacaagctc ttggtcaacg acgaacacga cctggtgcgc tacgaccccg gcatttccgt
     1561 cgtcgaaggc ggcagggcgg gttctaacgg ctttaccata cgcggcgtgg acaaagaccg
     1621 cgtcgccatc aacgttgacg ggctggcgca ggcggaaagc cgctcttccg aagccttcca
     1681 agaattgttc ggcgcgtacg gcaacttcaa cgccaaccgc aacacttccg agccggaaaa
     1741 cttttccgaa gttaccatca ccaaaggcgc ggactcgctc aaatccggca gcggcgcatt
     1801 gggcggcgca gtcaattacc aaaccaaatc cgcaagcgat tatgtttccg aagacaagcc
     1861 ctaccatttg gggataaagg gcggcagcgt cggcaaaaac agccaaaaat tcagcagcat
     1921 caccgccgcc ggcaggctct ttggtttgga tgccttattg gtttataccc gccgcttcgg
     1981 caaagaaacc aaaaaccgct cgaccgaggg caatgtcgaa attaaaaaca aagggtatgt
     2041 ttacgacccg aacaaaccat tcaacccgag tctgggcccc tcctcctacg tagccacagg
     2101 ggttgcgcgc tcccaacccg acccgcaaga atgggtaaac aaaagcaccc tgttcaagct
     2161 gggctacaat ttcaacgacc gcaaccgcat cggctggatt tttgaagact cccgcaccga
     2221 ccgttttacc aacgagctgt ctaatttgtg gacgagtctc acgggagaca taacgggcga
     2281 ctaccgccac cgccaagacg tgagctaccg ccgccgctcc ggcgtcgaat acaaaaacga
     2341 attggaacac ggcccgtggg acagcctcaa gctgcgctac gacaagcagc gcatcgatat
     2401 gaacacctgg acttgggaca tcccgaaaga ttacgataca aacggcatca acggccaggt
     2461 ttaccattcg ttccggcata tccgccaaaa caccgcgcaa tggactgccg attttgaaaa
     2521 acaactcgac ttttccaaag ccgtttgggc ggcgcaatac ggcttgggcg gcggcaaagg
     2581 ggacaatgcc aactcggatt acagctattt cgcaaaactg tacgacccca aaatcctcac
     2641 taccaaccaa ggcaaaatca caatgctgat cgaaaaccgg tcgaaatata aatttgccta
     2701 ttggaacaat gcgtttcact tgggcggcaa cgaccgcttc cgcctgaatg cgggcatacg
     2761 ctacgacaaa aacagcagca gcgcgaaaga cgatccgaaa tacaccaccg ccatccgggg
     2821 acagattccc catttgggtt cggaacgcgc gcacgccggc ttcagctacg gcacggggtt
     2881 cgactggcgg tttaccaagc atctgcactt gttggcaaaa tacagcaccg gcttccgcgc
     2941 gccgacttcg gacgaaacct ggctgctgtt cccacacccc gatttctacc tcaaagccaa
     3001 tcccaacctg aaagccgaaa aagccaaaaa ctgggaattg ggtctggcgg gcagcggcaa
     3061 agcgggcaac ttcaagctct cgggcttcaa aaccaaatac cgcgacttta tcgaattgac
     3121 gtatatgggc gtttcgtcag acaaccctaa caagcccacc tacgccccgc tttcagacgg
     3181 tacggcattg gtcagctcac ccgtttggca aaaccaaaac cgcaccgccg cctgggtgaa
     3241 aggcatagag tttaacggca cttggaacct cgacagcatc gggctgccca aagggctgca
     3301 caccggcctc aacgtcagct acatcaaggg cagggcaacg caaaacaacg gcaaagaaac
     3361 gcccatcaac gcgctttcgc cgtggacggc ggtttacagc ctgggctatg acgcgccttc
     3421 caaacgctgg ggcatcaacg cctacgccac gcgcaccgcc gccaaaaagc cgtccgacac
     3481 cgtccacagc aacgacgact tgaacaaccc gtggccttat gccaaacaca gcaaggccta
     3541 tacgctgttc gacctttccg cctacctcaa catcggcaaa caggtaacgc tccgcgccgc
     3601 cgcgtacaac attaccaaca agcagtacta cacttgggaa tctttacgca gcatccgcga
     3661 gttcggcacg gtcaaccgcg ttgacaacaa aacccacgcc ggcatccaac gctttacctc
     3721 gccgggcagg agctacaatt tcaccatcga agcgaagttc taagctcctg atgtgccaat
     3781 gccgtctgaa acccgatgat ttgggtttca gacggcattt tttacggtcg caccgccgcc
     3841 ccctttcagg cttgtaccaa gcccaatccc gcaactctcc gtcattcccg caactctccg
     3901 tcattcccgc gactctccgt cattcccacg gaagtgggaa tctagaaacg caaagctgca
     3961 agaatttatc ggaaatgact gaaactcaac gaacctggat tcccgcctgc gcgggaatga
     4021 cgaagctatc cgcacagaaa cctgcaccac gtcattccca cgaaagtggg aatccagaac
     4081 gtaaaatctg aagaaaccgt tttatccgat acgtttccgc accgacagac ctagattccc
     4141 gctttcgcgg gaatgacggc ggaaaggttg ctgtttttcc gataaattcc tgccgctctt
     4201 cgtttttggg atggcgggaa ataaaacaaa agcgcgcgta tcaaaaaaca acaatgcaaa
     4261 gaacggtgtt ggcaatttta aggtttgcgc cttagccgca cttattcgca aaaaaaccgc
     4321 acggcgttga ccgtgcggct tgttgtctga aggcttcaga cggcattgct tacatcatgc
     4381 cacccatacc gcccataccg cccatgtcag gcatagcagg tttttcttca gggatttcgg
     4441 caatcataca atcagtggtc agcatcaggc cggcgataga tgcggcgtgt tgcagcgcgg
     4501 aacgggttac tttggcgggg tcgagtacgc ccatttcgat catgtcgccg tattcgccgg
     4561 agcctgcgtt gtaaccgtag ttgcctttgc cttccaacac tttgttcacc accacgctag
     4621 gttcgccgcc tgcgttggca acgatttggc gcagcggaga ctcaacggcg cgcaagacga
     4681 tttgtacgcc tgcgtcttgg tcggcattgc cggtgtgcag gttttccaga gcggcacggg
     4741 cgcgcaacag ggcgacaccg ccgcctgcaa ccacgccttc ttcaacggct gcgcgggtag
     4801 cgtgcagcgc gtcttccacg cggtcttttt tctctttcat ttcgacttcg gtcgcggcac
     4861 cgactttgat cactgccacg cctcctgcca atttggcaac gcgctcttgc agtttttctt
     4921 tgtcgtaatc gctggtcgcg gtttcgattt gttggcggat ttcggcaaca cgcgcttcga
     4981 tttgggctgc gtcgccgaag ccgtcgatga tggtggtgtt ttctttaccg atttcgatgc
     5041 gtttggcttg acccaagtcg tccaaagtcg ctttttccag agacaggccg acttcttcgg
     5101 aaatcaccac gccgccggtc aggatggcga tgtcttgcaa catcgctttg cggcggtcgc
     5161 cgaagccagg ggctttgacg gcaacggttt tcaggatgcc tcggatgttg ttcacgacca
     5221 aagtcgccaa ggcttcgcct tctacgtctt cagcgataat caacagcgga cggctggctt
     5281 ttgccacttg ttccaaaaca ggcagcaagt cgcggatgtt gctgattttt ttgtcgaaca
     5341 acaatacaaa cggattgtcc aaagcagcga tttgtttttc agcgtcgttg atgaagtaag
     5401 gagacaagta gccgcggtcg aactgcatac cttcaaccac gtccagctcg ttttccaaag
     5461 atttgccgtc ttcaacggta atcacgcctt ctttgccgac tttttccatc gcttcggcga
     5521 taatcgcgcc gacttgttcg tcagagttgg cggaaatcga gccgacttgg gcgatttctt
     5581 tagaagtgtc gcaaggtttg gcgatgtttt tcagctcgtc aaccaaagcg gcaacggctt
     5641 tgtcgatacc gcgtttcagg tcggtcgggt tcataccggc ggtaacgtat ttcatacctt
     5701 cggcaacgat ggattgcgcc aatacggtgg cggtagtcgt accgtcgccc gccacgtcgt
     5761 tggttttgga cgcgacttct ttcaccattt gcgcgcccat attttcaaac ttgtctttca
     5821 attcgatttc tttggcgacg gttacgccgt ctttggtgat gtgcgggccg ccgaatgcgc
     5881 ggtcaaccac cacgttgcgg cctttgggac ccaaggttac gcgtacggcg tttgccagaa
     5941 tgttcacgcc gtttaccatt ttttgacgga cttcattgcc gaactgtacg tcttttgctg
     6001 ccatttcaat tctccaaaaa tcattaaaac tgtctgataa aaccgtttat gccgtctgaa
     6061 ggcggtttgc cgtttcagac ggcatcgtgt ccgtatttat ttttcaacga tgccgaaaat
     6121 atcttcttcg cgcattacca acagctcttc gccgtcggct tttacggttt ggccgctgta
     6181 tttgccgaag atgattttgt cgccgacttt gacatccagc ggacggcgcg cgccgtcttt
     6241 accgattttg cccgcgccca cggcgatgac ttcgcccatg tcgggttttt cggcggccgc
     6301 acccggcaaa acgatgcccg atgcggtttt ttcttcagct tccaagcgtt tgacgacaac
     6361 gcggtcgtgt aaaggacgga tggtcatatt tatgctccga taaaatgatt tgaaaacaat
     6421 catctgcccg aacggttcgg acagattgaa gtggaaaccg ggctgccgcc tgccggcccg
     6481 cccgtataag tcggcaaaat tagggtgtgt gcgggcaaat tcaagtgagg cggaaaaaat
     6541 ttatttccgc cgttttttat agtggactaa atttaagggg ctgtactaga ttagcagata
     6601 tgttaccctc gaaatatgaa gataacgcac tgcaaattaa agaaaaaagt acagaaagaa
     6661 ctgctccgtt tttttgtact cgaagttacc gcccgttctg ccgccgatat tttgggtatc
     6721 catcccaatt cggcagcact gttctaccgt aaaatccgca cggttatcaa ccatcatttg
     6781 gccctggctg ccgatgaggt ttttgaggat cctgtcgagc cggacgaaag cgatttcggc
     6841 ggacggcgta aaggcagacg tggtcgcggt gcggcaggaa aagtggttgt cttcggcatt
     6901 ctgaaacgca acggacgggt ctataccgtt gtggtggata atgccaagtc tgaaacgtta
     6961 ctccctgtca tcaaaaagaa aatcatgccg gacagtattg tttataccga tagtctgagc
     7021 agctgcgaca agttggacgt gagcggtttt atccattacc gcatcaacca ttccaaggaa
     7081 tttgcagacc gtcagaacca cattaacggc attgagaatt tttggaatca ggcaaaacgc
     7141 gtcttgcgaa aatacaacgg aatcgattgc aaatctttcc cgctgttctt gaaagaatgc
     7201 gaatttcgat ttaacttcgg cacaccgtct caacagctta aaatcctgcg ggattggtgt
     7261 gggatttagg gctaatctag tacagcccct aaaatttttc gttttcaagc cttcaccgct
     7321 tgccatcagc gttaaatttt tttacgataa gcacatagat tgtaaacaat cggccacaag
     7381 ccggtttgtt ttttcagaag acattatccc tgtcagacgc tgtttctata tatttcgcct
     7441 ataatggctt gtttttaata aataattcaa gaggtatcaa cgtgtctgat tccaagacga
     7501 aagaacgcgc cacattcggc acgcgccgcg cgtttatgat tgccgccatc gggtccgccg
     7561 tcggcttggg caatatttgg cgtttcccct atattgcttt tgaaaacggc ggcggcgcgt
     7621 tcatcctgcc ctatctggtc gcgcttctga cggcgggcat cccgctgctg ctgctcgatt
     7681 atgccatcgg ccaccgttac cgtggttctg cgcccttggc tttccgccgc ctcggacgat
     7741 ggtttgagcc ggtcggctgg tggaacgtga tgaccaatat cgtcatctgc atctattacg
     7801 cggtaattat cggttgggcg gcaagctata cctattattc ggtcaacgcc gcctggggtg
     7861 cggatccgca gggttttttc tttaaggact tcctgcaaat ggcgggcccg gaagccttgg
     7921 gtttggattt tgtcggcaaa gtcgccggtc ctttggcggg cgtgtgggtt tttaccgccg
     7981 ccattatggc gttgggcgtg caaaagggcg tggcgcgcgc ctcgtcgttc tttatgccgc
     8041 tgcttttggt gatgtttttg attatggtcg gcatttcact aaccctgccg ggtgcggcaa
     8101 agggcttgga cgcattgttt acgcccgact ggtcgaaact cgccgattcc aaggtctggg
     8161 tggcggcata cgggcagatt ttcttttcgc tttccatctg cttcggcatt atggttacct
     8221 attcttctta tttgaagaaa aaaaccgact tgggcggaac ggggctggtg gtcggttttg
     8281 ccaacagcag ctttgaactg ctcgcgggca tcggcgtgtt tgccgcattg ggctttatgg
     8341 cgcaggcggg cggtaaggcg gtcaacgagg ttgcctcagg cggcatcggt ttggcgttta
     8401 tcgcctttcc gaccattatc aaccaggcac cgatgggctg gctgatcggc atattgtttt
     8461 tcggttcgct ggtgttcgcc ggcgttacgt cgatgatttc catccttgaa gtgattgtgg
     8521 cggcgattca ggacaagctg aacatcgggc gcgtcaacgc cacgctgctg gtctgcattc
     8581 cgatgggcat tgtttccacg ctgctgttcg gtacggcgac ggggctgccg gttttggacg
     8641 tgatggacaa attcgtcaac acctacggca ttgttgccgc cggctttgtt tatgttgccg
     8701 ccatcatcat cagcggcagg ctgccggaat tacgcaagca cctgaacgct ttgtcctcca
     8761 tccgcatcgg cggcttgtgg acggtctgcg tcgtggttac cgtcgtgatg ctcggctata
     8821 tgctgtttaa agataccagc ggcctgatgg agaaaaatta cgaaggttat ccggatggtt
     8881 tcctcagtat tttcggctgg gggatgtcgg cggcgttggt cgtgttcggg ctgctgctgt
     8941 cgttgctgcc ttggaaacac ggtcaggatt tcaacgtcaa agacgaacac gaacatgaac
     9001 aaggagaaga aaaatgagta cttccgccat tgtgatgatg attgtctcaa tcgtgataat
     9061 ctggggaggg ctgctgcttt ccctgttaag gctgccgaac gagtaagcct ttagagcgtt
     9121 aaaaatgccg tatgaaccgc ttcagacggc attttgatgg tcggcttaca ggcaggcgag
     9181 ttcgtttgcc atttgctgtt ccaaggtttc gcgccggcgg atgagtcggt attcgttgcc
     9241 gtccaccaac acctctgccg cacggttgcg cgcgttgtaa ttgctcgcca tactggctcc
     9301 gtatgcgccc gcgctgcgga taagcagcaa atcgccttct tcgcaggcga tggtgcggtc
     9361 tttgccgagg aagtcgccgg tttcacaaat cgggccgacg atgttggcgg tcagcggcgc
     9421 gatgtttttt ggttctaccg cttcgatgtg gtggtaggca tcgtatagtg cggggcgcat
     9481 caaatcgttc atcgccgcat cgaccatcac aaagtttttc tcttcgccgt gtttgacgaa
     9541 ttcgacacgt gtcaataacg cgcctgcgtt gccgaccaag ctgcggccgg gctcaaggat
     9601 gagtttcaga cggcgtgtcc ccatcagttt ttgaaccgcc cgggcatacg cgcccaagtc
     9661 gggcacattt tcgtcttggt aaacgatgcc gacgccgccg cccaagtcca aatgttccaa
     9721 aacaatgcct tcggcggcaa gagcgtcaac caaaatcaaa atgcgttcgc aggcttcgat
     9781 gagcgggctt aagtcggtca gttgcgagcc gatgtggcag tcgatgccga tgattttcaa
     9841 attgggctgt tgtgcggcat ggcggtaggc ttcgagcgcg tcggcgtagg cgatgccgaa
     9901 tttgttggct ttcagtcctg tggagatgta gggatgggtt ttcgcatcga catcggggtt
     9961 gacgcgcagg gagacgggcg cggttttacc caaacgcgcg gcaacttttt gaatgcggtc
    10021 gatttcgggg atgctttcca tattgaagca ttttacgccg gcattcagcg cgaacttgat
    10081 ttccgcctcg cttttgccta cgcctgaaaa tatggttttt gctgcatcgc cgcctgccgc
    10141 caaaacgcgt gccaattcgc cgccggacac aatgtcaaaa ccgctgccca gcgaggcgaa
    10201 gtgtttgatg atgctcagat tgccattggc tttgacggca tagcagacga gtggggaaag
    10261 cgcggcaaag gcggtttggt agtgttcaaa cgcttcggtc agcgcggatt ggctgtacac
    10321 ataaagcggc gtgccgagtg cttcggcaag gcgggggtag gggacttgtt cgcaaaacag
    10381 gggcatattt tcgttttcat ttttgggttg gtggggcgga ttgcggtgtg ctttgaagct
    10441 gcaaaccggt ttggattacg ccgaaacgcg ccttgtcgcc ttctttgggc aggtagaggt
    10501 cgcctttgta accgcaggcg gaaagcagga gggcggttgc cgccgcaaaa aatacgccgt
    10561 atttcatcgg taaacttcct tcataagcgc gaatgtggca agattcggca tcttaaacaa
    10621 aaaacacgca aaaagccatg atgaccgaaa gcgagtttat ccgcgcgagc gaagcattat
    10681 ttgaacacat cgaagaccaa atcgacgaaa acggctggga tttcgactgc cggtttgccg
    10741 gaaacgtcct gaccatcgaa gccgaaaacg gcacgcaaat catcgtcaac cgccacacgc
    10801 ccaaccaaga attatggatt gccgccaaaa gcggcggcta ccatttcgcc gaacaaaacg
    10861 gcaaatggct ggcaacgcgc gacaaccgcg atttttacga cgttttaaac gaagccctga
    10921 gcgcggcttc gggcgaagcg gtaaagattg ccgaattgta atttgagtgt tatcacggag
    10981 agaaaaaatg aacacacgtc ccttttattt cggacttata tttatcgcga ttatcgctat
    11041 atttgctaac tatttaggaa acactgattt ttcccatcat tatcatatca gtgctttaat
    11101 tattgctatc ttgctgggaa tggcaatcgg caataccatt tatccgcaat tttcaacaca
    11161 agtggaaaaa ggcgttttgt ttgccaaagg cacgcttctt cgcactggca ttgtgctgta
    11221 tggtttccgc ctcacttttg gcgatattgc cgatgtagga ttaaatgcgg ttgtcactga
    11281 tgcaatcatg ctaatttcaa ccttcttttt taccgtactt ttaggcattc gttatctaaa
    11341 aatggataaa caattggttt atctcactgg ggcaggttgc agcatttgcg gtgcggcagc
    11401 ggttatggcg gcagagtctg ttaccaaagc agaatcccat aaagtttcag tggcgattgc
    11461 catagtggtc attttcggga cgcttgctat ttttacttac cccttgttct acacgtggtc
    11521 acaagattta attaacgccc atcaattcgg tatttatgtt ggttctagtg tacacgaagt
    11581 ggctcaagtg tatgcgattg gggaaaatat tgatcctatc gtggcgaata ctgccgtcat
    11641 ttccaaaatg atccgagtga tgatgctcgc ccccttttta ttaatgcttt cttggttatt
    11701 aacacgtagt gatggggtat cagaaaatac ctcacacaaa attacaattc cttggtttgc
    11761 tgtacttttt attggtgttg ccatttttaa ttcttttgat ttattaccaa aagaactcgt
    11821 gaaattattc gttgaaatcg attctttctt attaatttca tcaatggctg cgcttggctt
    11881 aacgacgcac gcaagcgcaa tcaaaaaggc aggattaaaa ccgtttgttt taggaatact
    11941 aacttattta tggctagtgg ttggtggatt tttagtgaac tatggaatat caaaattaat
    12001 ataaaattca ctaaagagag cgttacccaa tggcacaatt accgctatat ctgacttctg
    12061 aaatcaaaga ctttactgtc ggcacgccta aagttttgga atcattttcc aaacatatcc
    12121 cttatggtgt tgtctttgaa gacgacggcg acacgggcta cttctatgcc gcttcgcaag
    12181 acgggattct agatgccttg cacatctaca atgtcgaaga tgtatccgac aaacatatcc
    12241 ccaatcatgt cttgatttta tgggatgatg cctgcaccat agccgcattg agtatcaacg
    12301 actacattca tgccgtctat gattttgtcg aacaggcagg atattgccgc aacggcttcc
    12361 ctgaaacagg cggcgaatgg gtgaaagtcg aaaaccgcgt cttggacgat gaattgctgg
    12421 acaaaatctt atcccgaaaa tctacataac ctactcaaaa ggataacaaa atgcccctgt
    12481 tagacagttt caaagtcgac cacacccgta tgcacgcccc tgccgtacgc gtggcgaaaa
    12541 ccatgaccac gcccaaaggc gataccatta ccgtattcga cctgcgcttt tgcgttccta
    12601 acaaagaaat cctgcctgaa aaaggcatac acacgctgga gcatttgttc gcaggcttta
    12661 tgcgcgacca cttaaacggc aacggcgtcg aaatcatcga catttccccg atgggctgcc
    12721 gcaccggttt ttatatgagc cttatcggca cgccttccga acagcaggtc gccgatgcgt
    12781 ggctggcttc gatgcaggat gttttgaatg tcaaagacca aagcaaaatc cccgagttga
    12841 acgaatacca atgcggcact tatcaaatgc actcgctcgc cgaagcgcaa caaatcgcgc
    12901 aaaacgtgtt ggcgcgcaaa gtggcggtga acaaaaatga agagctgacg ctggatgaag
    12961 ggctgctgaa cgcctaatcc gccaaaaatg ccgtctgaac aagggtttca gacggcattt
    13021 gccttttccg ttataatctg gggttgtgcg ggggcgggtt ttaagccggc atcgtccttc
    13081 cctatttttt tctgtccctt atcggtttta agcgggtttt ttatgtccaa cagacctaca
    13141 ctcctcctcg ttgacggatc gtcctacctc taccgtgcgt atcacgcgat ggggcaaaac
    13201 ctgaccgcac ccgacggtgc accgacgggt gcgctgtatg gcgtattgaa tatgttgcgc
    13261 cgtttgcggt cggaatatcc gcacgattat tgcgcggtgg tttttgatgc gaaaggcaaa
    13321 aatttccgcc atcaaatgtt tgaagaatac aaggcgacgc gcccgccgat gcccgacgat
    13381 ttgcgtccgc aggcggaagc tttgccggat ttggtgcgcc tgacgggctg gccggtattg
    13441 gtgattgggc aggtggaagc ggacgatgtg atcggcacgc tggcaaaaca gggggcggaa
    13501 catggtttgc gagtcattgt ttcgaccggc gataaggaca tggcgcagtt ggtggatgag
    13561 cgcgttacgc tggtgaacac gatgagcgga gaaacgctgg atattgaagg cgtgaaggca
    13621 aaattcggcg tgcgccccga ccaaatccgc gattatctcg cgctgatggg cgacaaggtg
    13681 gacaacgtgc cgggcgtgga aaaatgtggc ccgaaaacgg cagtgaagtg gctggaagcc
    13741 tacggctcgt tgcaaggcgt gatagaacac gcttcggaaa tcaagggcaa agtgggcgag
    13801 aatctgcgtg ccgccctgcc gcgcctgccg ctgtcgtatg atttggtaac gattaaaacc
    13861 gatgtggact tgcacgccga gctttcagac ggcctcgaaa gcctgcgccg taccgcgccg
    13921 aaatgggcgc agctggttgt cgatttcaaa cgctggggct tccgcacctg gctgaaggaa
    13981 gcggaaagcc gtatgcacga agcggcggac ggcgatttgt tcggcagcga tacgataggc
    14041 gaacaggcgg cgttggatat ggaaacgtcg tctgaaaggc tacctgaaaa agccgttgcc
    14101 cccgaaaagc tggattatca ggccgttacc accgaagcgc agtttgccac tttgttagac
    14161 aaattgtcgc aggcggaaaa aatcggcatc gacacggaaa ccacttccct agacgcgatg
    14221 aacgccgcgc tggtcggcat cagcatcgcg ttccaagcgg gcgaagcagt ttacatcccc
    14281 gtaggacaca gcctgaccgc cgcgccagaa cagcttgatt tgcaagacgt attaggccat
    14341 ctgaaaccgt atttggaaaa tcctgccctg aaaaaaatcg ggcaaaacct caaatacgac
    14401 caacacgttt tcgccaatta cggcatcgcc ctgaacggca tcgccggcga cgccatgctc
    14461 gcttcctaca tcatcgagag ccatctcgga cacggcttgg acgaattgtc cgaacgctgg
    14521 ctcggcttgg aaaccattac ctacgaatcg ctgtgcggca aaggcgcgaa gcaaatcggt
    14581 tttgccgatg tcgccatcgg gcaggcgacc gaatacgccg cccaagacgc cgatttcgcc
    14641 ctgcgcctcg aagcgcacct gcgcgcgcaa atggacgaaa aacagcttga aatgtatgaa
    14701 aaaatggagc tgcccgtcgc gcaggtattg tttgaaatgg aacgcaacgg tgtgcaaatc
    14761 gaccgcgccg aactcgcccg ccaaagcgcg gaactcggcg ccgagctgat gaagctcgaa
    14821 caagaagcct acgccgccgc agggcagccg ttcaacctca actcgcccaa acagctgcaa
    14881 gaaatcctgt tcgacaaaat gggcatcccg acaaaagggc tgaaaaaaac cgccaagggc
    14941 ggcatttcca ccaacgaggc cgtgctcgaa cagctcgcgc ccgactaccc cttgcccaaa
    15001 atcatcctgc aaaaccgcag cctggcgaaa ctcaaatcca cctacaccga caaactgccc
    15061 gacatgattt ctccccggga taaccgcgtg cataccacct acgcccaagc cgtcgccatt
    15121 acaggacgct tggcaagcaa caaccccaac ctgcaaaaca tccccatccg caccgccgaa
    15181 ggccgccgcg tgcgccgcgc ctttaccgcg ccaccgggca gcgtcatcgt ttccgccgac
    15241 tattcccaaa tcgaactgcg catcatggcg cacctctctg gagacaaaac cctcattgcc
    15301 gcattccaaa acggcgaaga cgtacaccgc cgcaccgccg ccgaagtgtt cggcactgcg
    15361 cccgaaaacg tctcgcctga gcaacgccgc tacgccaaaa ccatcaactt cggcttaatc
    15421 tacggtatgg ggcaatacgg ccttgccaaa tcattgggca tcgacaacct ttccgccaaa
    15481 aactttatcg accgctactt cgcccgctac cccggcgtcg ccgaatacat gcagcgcacc
    15541 aaagaacaag ccgccgccca aggcttcgtc gaaaccctgt ttggccgccg cctctacctg
    15601 cccgacatcc gcaacaaaaa cgccaacgcc cgcgccggag ccgaacgcgc cgccatcaac
    15661 gcccccatgc aaggcaccgc ctccgacctt atcaaacgcg ccatgataga cgtgtcccgc
    15721 tggctggttt cagacgacct gaagagcaaa ctgattatgc aggtgcatga cgaactggtg
    15781 ctggaagtcg ttgaaaccga actggatttt gtcaaagaaa aactgccgca gattatggcg
    15841 aaagtgggcg gcggattatt ggatgtaccg ctggtggctg aggttggcgt aggggagaat
    15901 tgggaagagg cacattgatt gaaaggtgtt attatgctat ctttatttaa ataaaattta
    15961 atttttggta tattttttct aaatgttcct a
//
LOCUS       DAWFLT010000039        15938 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWFLT010000039 DAWFLT010000000
VERSION     DAWFLT010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15938
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.039"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..79)
                     /locus_tag="WGR74_001238"
     CDS             complement(<1..79)
                     /locus_tag="WGR74_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ0905662.1"
                     /translation="MKPVVWAALLLCACTSNFGDREHQFL"
     gene            282..1610
                     /gene="miaB"
                     /locus_tag="WGR74_001239"
     CDS             282..1610
                     /gene="miaB"
                     /locus_tag="WGR74_001239"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ0905663.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="WGR74_001240"
     CDS             complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="WGR74_001240"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ0905664.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYNTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVAKLPKEGAAQMPSEKEPKPVAKPTYTQ
                     VFGKWLCDRAAADERLIAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGGKTAFIAFGSMVAPALAVAGKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="WGR74_001241"
     CDS             complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="WGR74_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ0905665.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQDLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCVWLVERGLLHTDP
                     TADIKPPKQSERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLVGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4804..5868
                     /locus_tag="WGR74_001242"
     CDS             4804..5868
                     /locus_tag="WGR74_001242"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ0905666.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            5888..6850
                     /locus_tag="WGR74_001243"
     CDS             5888..6850
                     /locus_tag="WGR74_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905667.1"
                     /translation="MPSEPPFGRYLIFASLTCLIDAVCKKRYHNQNVYILSILRMTRS
                     KPVNRTTFCCLSLTAALILTACSSGGGGVAADIGAGLADVLTAPLDHKDKGLQSLTLD
                     QSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLITLESGE
                     FQVYKQSHSALTALQTEQEQDLEHSRKMVAKRRFKIGDIAGEHTSFDKLPKDVMATYR
                     GTAFGSDDAGGKLTYTIDFAAKQGHGKIEHLKSPELNVDLAVAYIKPDEKHHAVISGS
                     VLYNQDEKGSYSLGIFGEKAQEVAGSAEVKTANGIHHIGLAAKQ"
     gene            6990..7667
                     /gene="tsaB"
                     /locus_tag="WGR74_001244"
     CDS             6990..7667
                     /gene="tsaB"
                     /locus_tag="WGR74_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ0905668.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGETRLFHQEAGSRQSELI
                     LPEIRTLFHDAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFNTPLIGIPTLD
                     AVASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRTADIALPEGHVFSDG
                     IGNAFALENRPPFAGKPDMPTAADFLNLAEEGGFPAVAAAHAELLYVRNKIALTVKEQ
                     AERKART"
     gene            7664..8104
                     /gene="rimI"
                     /locus_tag="WGR74_001245"
     CDS             7664..8104
                     /gene="rimI"
                     /locus_tag="WGR74_001245"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ0905669.1"
                     /translation="MNIRRPSPSDCAALAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRQGIASALLEYWYAHLPEGTQRLLLE
                     VRAGNTAAQALYAAHGFSIAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            8098..8832
                     /locus_tag="WGR74_001246"
     CDS             8098..8832
                     /locus_tag="WGR74_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ0905670.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPAPSAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVHTRKPAPETETPLPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGVLLDNILKAVGLDAAYVHKTSWVKTAAVGNPMP
                     SEQAVANALGQIARELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARNWQVLKQLKHALQAGGS"
     gene            8894..9535
                     /gene="pyrE"
                     /locus_tag="WGR74_001247"
     CDS             8894..9535
                     /gene="pyrE"
                     /locus_tag="WGR74_001247"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ0905671.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9609..10043
                     /locus_tag="WGR74_001248"
     CDS             9609..10043
                     /locus_tag="WGR74_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905672.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTLHDTPPATAATP
                     AAAPQVAVPPAAPARQEGFNWTIATLFALIVLIMQLSYLVIL"
     gene            10040..11350
                     /gene="argA"
                     /locus_tag="WGR74_001249"
     CDS             10040..11350
                     /gene="argA"
                     /locus_tag="WGR74_001249"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ0905673.1"
                     /translation="MIVPDLFVAHFREAVPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFYLDMLQTAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSTVAALEGGVHRVQILNGASDGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEISCLAVSPQAQDGGYGERLLAHIIDKARSMGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            11446..13461
                     /locus_tag="WGR74_001250"
     CDS             11446..13461
                     /locus_tag="WGR74_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ0905674.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEKPNRALLTLSKSGGDTAYTLEVDLEARELVEGGFHFP
                     AGKNQVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIDKDAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSAEGEAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWNRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAAGDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGIEVRQFWVMSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGVPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPQHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQREAADELACVLLFLKEFLG"
     gene            complement(13575..14531)
                     /locus_tag="WGR74_001251"
     CDS             complement(13575..14531)
                     /locus_tag="WGR74_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0905675.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRPFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            14767..15732
                     /locus_tag="WGR74_001252"
     CDS             14767..15732
                     /locus_tag="WGR74_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0905676.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKEAFEKAEPVAAGKE"
BASE COUNT         3549 a         4844 c         4370 g         3175 t
ORIGIN      
        1 gcaggaactg atgttccctg tcgccgaaat tgctggtaca cgcacacagc agcaatgccg
       61 cccatacaac cggtttcata cacatctccc attaaagcca aacattatac agccgtcccg
      121 accgattaaa ttcatatttt aaaacaatat cctgcctcca aaacccacat cgtgctataa
      181 tccacaccga ttttcagacg gcatcgtcat gccgtctgaa attttttcat tccaacaacc
      241 atcagccccg cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc
      301 gtaccttcgg ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg
      361 aagaacacgg cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca
      421 acacctgctc cgtgcgcgaa aaagcacagg aaaaagtgtt ctccgatttg gggcgcgtgc
      481 gtccgctcaa agaaaaaaac cccggcctta tcatcggcgt tgccggctgc gtcgcctcgc
      541 aagaaggcga aaacatcatc aaacgcgcgc cttatgttga cgtggttttc ggcccgcaaa
      601 cgctgcaccg cctgcccaaa atgattgtgg acaaagaaac cagcgggctg tcgcaagtcg
      661 atatttcctt ccccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtcgaaggcg
      721 gcgcggcatt tgtatcgatt atggaaggct gttccaaata ctgctcattc tgcgtcgtcc
      781 cctacacgcg cggcgaagaa ttctcccgcc cgctcaacga cgtattgaca gaaatcgcca
      841 accttgccca gcaaggcgtg aaagaaatca acctcttggg acaaaacgtc aacgcctatc
      901 gcggcgaaat ggacgacggc gaaatctgcg acttcgccac cctgctgcgc atcgtccacg
      961 aaatccccgg catcgaacgt atgcgcttca ccaccagcca cccgcgcgag tttaccgact
     1021 cgattatcga gtgctaccgc gacctgccca aattggtttc ccacctgcac ctgccgattc
     1081 aaagcggttc cgaccgcgta ttgagcgcga tgaaacgcgg ctacaccgct ttggaataca
     1141 aatccatcat ccgcaaactg cgtgccatcc gtcctgattt atgcctgagc agcgatttca
     1201 tcgtcggctt ccccggcgag accgaacgcg agttcgagca aaccttgaaa ctggtgaaag
     1261 acattgcctt cgacttgagc ttcgtgttta tttacagtcc gcgccccggc acgcctgccg
     1321 ccaacctgca cgacgacacg ccgcacgaag aaaaagtgcg ccgcctcgaa gccttgaacg
     1381 aagtcatcga agccgaaacc gcgcgcatca accaaaccat gatcggcacg gttcaacgct
     1441 gcctggtcga aggcatctcc aaaaaagacc ccgaccaact gcaagcccgt accgccaaca
     1501 accgcgtcgt caacttcacc ggcacgcccg acatgattaa ccaaatgatc gatttggaaa
     1561 tcaccgaggc ctacaccttc tccctgcgcg gcaaagttgt cgaagcctaa accctcacgc
     1621 cgaaaaaatg ccgtctgaag cgtttcagac ggcattttgc cttgtatcgg cagacgacgg
     1681 cgcggccggg cggcttaatt tgccgcatcc cgatccgaca gccacgcgcg cacacgccgt
     1741 tccaccgctt cggcactcaa gcccaaatcg tctaaaagtt ttttcggatc gccgtgtccg
     1801 gttacggtat cggcaacgcc caaaagcaaa acgggtttgc agatgccgtg tttcgccaat
     1861 acttccagca ccgcgccgcc tgcgccgccc tgttcggcgt tttcttcaag ggtaacgatg
     1921 cggtcgtggc ttcgggcaag gcggacaatc aactcttcgt ctatcggttt gacaaagcgc
     1981 atatcggcga cggtggcgtt cagttttccg gcgaccgcca atgcgggggc gaccatactg
     2041 ccgaaggcaa tgaatgcggt tttcccacct tcgcggcgga taatgccctt gccgatttcc
     2101 acggtttcca tgccgtctga aaccggcgcg cccgtacccg tgccgcgcgg atagcggacg
     2161 gcggcgggcg catccgcctg atagcaggtc gaaagcagca ggcggcattc gttttcatcg
     2221 ctcggcgcgg cgacaatcat attcggaatg caacgcaaga agctcaaatc gtacaaaccg
     2281 gcgtgggtcg ggccgtccgc gccgacgatg cccgcgcggt cgacggcaaa caaaacgggt
     2341 aggttttgca gggcgatatc gtgcaccagt tggtcgtagg cgcgttgtaa aaaggtggaa
     2401 taaatcgcca cgacgggctt catgccttcg cacgccaaac cgccggcaaa ggtaacggcg
     2461 tgctgctcgg caataccgac atcgaaataa cggtcgggga atcgttgttc aaactccacc
     2521 agtccgctgc cctcgcgcat cgcgggcgtg atggcgatca aacgctcgtc tgccgccgcc
     2581 cggtcgcaca gccatttgcc gaacacttgg gtataggtcg gtttggcgac gggcttgggt
     2641 tctttttcag acggcatttg cgccgcgcct tctttaggca gcttggcgac ggcgtggtat
     2701 ttgacggggt cgttttcggc gagtttgtag ccgttgcctt ttttggtgat gacgtgcaga
     2761 agctgagggc ctttgcggct gcgcaagtct ttcaatacgt ccaccagatt ttcgacgttg
     2821 tgtccgtcca cggggccggt atagcggaag ccgaagtttt caaacaaaga cagcgactgt
     2881 ttggcgtgtt cggcttcttc ggcaagggtt ttgattttgt gttcgacttt ttgggcaaac
     2941 tccatcgcgc cgggtatttt gtctaatacc ttgcccgttt gcgctttgac ggtactcaac
     3001 aggccgtgca tatcgcgcac gacgttgctg gcaaggtatt tcggcagcgc gccgacgttg
     3061 ggggaaatcg acatttcgtt gtcgttgagg acgaccagca aatccacatc catatcgcct
     3121 gcgcaattca aggcttcaaa cgcctgcccc gccgtcatcg cgccgtcgcc gatgatggcg
     3181 acgctgcggc ggtcgctgcc caagagtttg tctgccgccg ccatgcccaa cgccgcgccg
     3241 atggaggtgg aggaatgccc cacgccgaac gcgtcgtact cggactcgca acgtttcgga
     3301 aaacccgcca aaccgccgta ttggcgcatg gtgtgcatct ggtttttgcg tccggtaagg
     3361 attttgtgcg gatagctttg gtgtccgaca tcccacacca gcttgtcttc gggcgtgttg
     3421 tacacatagt gcagggcgat ggtcagctcg accgcaccta agttgctggc gaaatgaccg
     3481 ccggtttgac cgacggattc cagcagaaag gcgcgcaact cgccggcaag gcgcggcagc
     3541 tgttttttgt ccagacggcg caaatcttgc gggctgtcaa tcaggtcgag tagggggctt
     3601 gggttcatgg tgtgtctttt ttatgtgtcg tccgggtgca acggccaatt atatatcaag
     3661 agcgtgcggc tgacggctga ttttgccgta tgtcattcgt cctgccgctt ggcgcgcggg
     3721 tgggcttcgt catacaggcg ggcgatgtgg tcgaaatcga gcttggtata aatctgcgtg
     3781 gtcgaaaggc tgctgtgccc gagcagctcc tgcaccgccc tgatgtcgcg cgaagcctgc
     3841 aacaggtgtc cggcgtagct gtggcgcatc atatgcggcg aaacgtgcct gccgtcgccg
     3901 ttttgcgccg cccattgcgc caaacgtttt tggatttggc gttggctcag gcgcgtgccg
     3961 ttcctgccgg taaacagggc tttgccgtcc gatgccgtct gacgcagcgg cagatagttt
     4021 ttcagggctt ccacgctttt gccgaccagc ggcacctgcc gctgcttgcg ccctttgccg
     4081 gtaacgtgta cccacgcctc gtccaaatag acatcatctg cattcaagcc gtgtatctcg
     4141 ctcacgcgca aaccgctgcc gtacatcagt tcgaacaggg cgtggtcgcg caccgccagc
     4201 gggtcgccgc cgtccacggg caaatccagc atccggttca gccattcctg cggcagtgct
     4261 ttgggtacgc gctcggactg cttcggcggt ttgatgtcgg cggtcgggtc ggtatgcagc
     4321 agaccgcgtt ccaccagcca aacacaatac tgccgccaag acgacagctt gcgcgccagc
     4381 gtccgcgcat tcaaaccgcg ctgcgacagc cgccgcaacg ccgccgtaaa atcgcgccgc
     4441 gacaagtcct gcggcacgcc gcctgcatct tcagacggca tttgtgccaa cagcgcaaac
     4501 agttcttcca aatcgcgccg gtatgccgcc accgtgtgct ccgacttgcc ctcgcgcacg
     4561 atgttttcca aataagcgtc aaaatgcgcc gcaaacccgt ccaaaaccat aaccgcccca
     4621 cacaaatatc aaaaaaacag tgaaaacatt atcataaacg acacaccaaa accccgaacc
     4681 gcccaaaatc cgacccgtcc gcccgccgtt atcgttaaca taaaatcaat gagccgttta
     4741 tggttttttg ccgcaaaaaa cattataatc cgccttattt acctattgcc caaggagaca
     4801 caaatggcac tcgtatccat gcgccaactg cttgatcatg ctgccgaaaa cagctacggc
     4861 ctgcccgcgt tcaacgtcaa caacctcgaa caaatgcgcg ccattatgga agccgccgac
     4921 caagtcaacg cgcccgtcat cgtacaggcg agtgcaggtg cgcgcaaata tgcgggcgcg
     4981 ccgtttttgc gccacctgat tctggcggca gtcgaagaat ttccgcacat ccccgtcgtg
     5041 atgcaccaag accacggcgc atcgcccgac gtgtgccaac gctccatcca actgggcttt
     5101 tcctccgtga tgatggacgg ctcgctgatg gaagacggca aaaccccttc ttcttatgaa
     5161 tacaacgtca acgccacccg taccgtggtt aatttctccc acgcttgcgg cgtatccgtc
     5221 gaaggcgaaa tcggcgtgtt gggcaacctc gaaaccggcg aagccggcga agaagacggt
     5281 gtaggcgcag tgggcaaact ttcccacgac caaatgctca ccagcgtcga agatgccgtg
     5341 cgtttcgtta aagataccgg cgttgacgca ttggcgattg ccgtcggcac cagccacggc
     5401 gcgtacaaat ttacccgtcc gcccacaggc gacgtgttgc gtatcgaccg catcaaagaa
     5461 atccaccaag ccctgcccaa tacacacatc gtgatgcacg gctccagctc cgttccgcaa
     5521 gaatggctga aagtcatcaa cgaatacggc ggcaatatcg gcgaaaccta cggcgtgccg
     5581 gttgaagaaa tcgtcgaagg catcaaacac ggcgtgcgca aagtcaacat cgacaccgac
     5641 ttgcgccttg cttccaccgg cgcggtacgc cgctaccttg ccgaaaaccc gtccgacttc
     5701 gatccgcgca aatacctgag caaaaccatt gaagcgatga agcaaatctg cctcgaccgc
     5761 tacctcgcgt tcggttgcga aggtcaggca ggcaaaatca aaccggtttc cttggaaaaa
     5821 atggcaagcc gttatgccaa gggcgaattg aaccaaatcg tcaaataaca ggttgccggt
     5881 aaacaaaatg ccgtctgaac cgccgttcgg acggtatttg atttttgctt ctttgacctg
     5941 cctcattgat gcagtatgca aaaaaagata ccataaccaa aatgtttata tattatctat
     6001 tctgcgtatg actaggagta aacctgtgaa ccgaactacc ttctgttgcc tttctttgac
     6061 cgctgccctg attctgaccg cctgcagcag cggagggggc ggtgtcgccg ccgacatcgg
     6121 tgcggggctt gccgatgtgc taaccgcgcc gctcgaccat aaagacaaag gtttgcagtc
     6181 tttgacgctg gaccagtccg tcaggaaaaa cgagaaactg aagctggcgg cacaaggtgc
     6241 ggaaaaaact tatggaaacg gcgacagcct taatacgggc aaattgaaga acgacaaggt
     6301 cagccgtttc gactttatcc gtcaaatcga agtggacggg cagctcatta ccttggagag
     6361 cggagagttc caagtgtaca aacaaagcca ttccgcctta accgcccttc agaccgagca
     6421 agaacaagat ctagagcatt ccaggaagat ggttgcgaaa cgccggttca aaatcggcga
     6481 catagcgggc gaacatacat cttttgacaa gcttcccaaa gacgtcatgg cgacatatcg
     6541 cgggacggcg ttcggttcag acgatgccgg cggaaaactg acctatacta tagattttgc
     6601 tgccaaacag ggacacggca aaatcgaaca tttgaaatcg ccggaactca atgtcgatct
     6661 ggccgtcgcc tatatcaagc cggatgaaaa acaccatgcc gtcatcagcg gttccgttct
     6721 ttacaaccaa gacgagaaag gcagttactc cctcggtatc tttggcgaaa aagcccagga
     6781 agttgccggc agcgcggaag tgaaaaccgc aaacggcata caccatatcg gccttgccgc
     6841 gaagcaataa ccattgtgga aatgccgtcc gaacacgata atttaccgtt cggacggcat
     6901 ttttgcgcca taccgcccta ggacacgacg gcacggcagg caagccggtt tcactatata
     6961 atgccgtctg aaccaacgag aaccacacca tgcaagccga tttcaaccgc cccgtcctcg
     7021 ccgtcgatac cggaacttcc tatttgtcgc tcgcgctgcg tgccgacggc gaaacccgtc
     7081 tgttccatca ggaagccggc agccgccagt ccgaactgat tctgccggaa atccgcacgc
     7141 tgtttcacga tgcgggcatt accgccgccg atttgggggc gattgtttat gcgaaaggcc
     7201 ccggcgcgtt taccggactg cgtatcggta tcggcgtggc gcaaggtttg gcaacgccgt
     7261 tcaatacgcc tttaatcggc atccccacgc tcgatgccgt cgcctcgctg ccgccgccgc
     7321 aaagctgcat actcgccgct gcggacgcac gtatgggcga agtgttttat gcgtggttcg
     7381 acacgctgaa ccgccgccgt ttgagcgatt atcaagtcgg taggacggca gacattgccc
     7441 tccccgaggg acacgtcttt tcagacggca tcggcaatgc gttcgcgctg gaaaaccgtc
     7501 cgcccttcgc aggcaaaccc gatatgccga ccgccgccga ttttctcaat ctggcggaag
     7561 aaggcggttt tcccgccgtc gctgcggcac acgccgagct gctctacgtc cgcaacaaaa
     7621 tcgccttgac tgtcaaagaa caggcggaac ggaaagcccg aacatgaaca tccgccgccc
     7681 ctcaccgtcc gactgcgccg cactcgccgc gctcgatgcc gtctgcaacc cgtccgcgtg
     7741 gacgcaacgc caatttgagt ccgcactggt ttcgccgtcc gaacaggttt tccttgcgga
     7801 aaaagacggc gggattgccg cctttatcgt ttggcagaac ctgcccgacg aatccgaact
     7861 gcacctgatt gccaccgcgc ccgaatgccg ccgccaagga attgcgtccg ccctgctcga
     7921 atattggtac gcacacctgc ccgaaggcac gcaacgcctg ctgctcgaag tccgcgcagg
     7981 caacaccgcc gcacaggcac tgtacgcggc gcacggcttc agcattgcgg gcaggcggaa
     8041 aaactattac cgtacagccg acggtaaaac cgaagatgcc gtcttgatgg agaaaatatg
     8101 ttaagcgcgc gctacctcca cctgcacgaa gctttgggtt taggcccgat gtggctgaaa
     8161 caggccgccg ccgtcctgcc gcccaaaaac acacccgcac cctcggcaca ggcacgtcct
     8221 caaaccgtcc gcgccgcccc gatccgccct tcccaacccc ataacggtca ggcacggctc
     8281 gaaacgatga aagcgttgga aaccgccgcc gtacatacgc gcaaacccgc gcctgaaacc
     8341 gaaacgcctc tgcccggcct ttcagacggc atcgcccccg ttcccgccgc ttcgggcatc
     8401 accaagcttg ccgtcgtcag cctttgccca ccgatcgagg atgcggttta cgggcaactg
     8461 ttccacggca aagcaggtgt tctgcttgac aatatactca aagccgtagg actggatgcc
     8521 gcttatgtac acaagaccag ctgggtgaaa accgccgccg tcggcaaccc gatgccgtct
     8581 gaacaggccg tcgcgaatgc gctgggtcaa atcgccaggg aactcgacgg ctgccgtgcc
     8641 cccgccgtcc ttttcctcgg gcaggctttt gtccagcctg aacggcaaac gatgattgaa
     8701 actttgtgcg gcagccgtcc cttcttcatc atcgaccatc ccgctcggct tttacgccaa
     8761 cccgaactta aagcccgaaa ctggcaggtg ttgaaacagt tgaaacacgc cttgcaggca
     8821 ggcggcagtt gaagcaggcc gcacggggcg gtagaatcgc aactgcgtcc caatatctga
     8881 cagaaagcac aaaatgaccg atttccgcca agatttcctc aaattctccc tcgcccaaaa
     8941 tgttttgaaa ttcggcgaat tcaccaccaa agccggacgg cggtcgccct atttcttcaa
     9001 tgccggcctc tttaacgacg gcttgtccac gctgcaactg gcaaaattct atgcacaatc
     9061 catcattgaa agcggcatcc gattcgatat gctgttcggt cccgcctaca aaggtattat
     9121 tttggcggcg gcaaccgcga tgatgctggc ggaaaaaggc gtgaacgtcc cgtttgccta
     9181 caaccgcaaa gaagccaaag accacggcga aggcggcgtg ttggtcggcg cgccgcttaa
     9241 agggcgcgtg ctgattatcg acgacgtgat ttccgccggc acatccgtac gcgaatcgat
     9301 caaactgatt gaagcggagg gtgcaacccc cgccggtgtc gccatcgcgc tcgaccgcat
     9361 ggaaaaaggc acgggtgaat tgagcgcggt tcaggaagtg gaaaaacaat acggtctgcc
     9421 cgtcgccccc atcgccagcc tgaacgattt gtttattctg ttgcaaaaca accccgaatt
     9481 cggacagttc ctcgaacccg tccgagccta ccgtcggcag tacggcgtag aataaaaaca
     9541 aagcatatgc cgtccgaacc gccttacgcc tcagacggca tcaaacctga cacacacgag
     9601 gaaataccat gcccgcctgt ttctgccccc actgcaaaac ccgtctctgg gtcaaagaaa
     9661 cccaactcaa tgtcgcccaa ggcttcgtcg tctgccaaaa atgcgaagga ctgtttaaag
     9721 ccaaagacca tctggcaagc acgaaagaac ccatattcaa cgatttgccc gaggctgttt
     9781 cggatgtcaa actcgttcac cgcatcggca cgcgcgccat tggcaagaaa cagatttccc
     9841 gcgacgaaat cgccggcatc ctcaacggcg gtacaaccct gcacgatacg ccgcccgcaa
     9901 ccgccgccac ccctgccgcc gcaccgcagg ttgccgtacc gcccgccgcg cctgcccgtc
     9961 aggaagggtt caactggacg attgcaaccc tgtttgccct tatcgtcctc attatgcagc
    10021 tttcctacct cgtcatccta tgatcgtgcc cgacctcttt gtcgcccact tccgcgaagc
    10081 cgtcccctac atccgccaaa tgcgcggcaa aacgctggtc gccggcatag acgaccgcct
    10141 gctcgaaggt gataccttaa acaagctcgc cgccgacatc gggctgttgt cgcaactggg
    10201 catcaggctc gtcctcatcc acggcgcgcg ccacttcctc gaccgccacg ccgccgcgca
    10261 aggccgcacg ccgcattatt gccggggctt gcgcgttacc gacgaaacct cgctcgaaca
    10321 ggcgcagcag tttgccggca ccgtccgcag ccgttttgaa gccgcattgt gcggcagcgt
    10381 ttccgggttc gcgcgcgcgc cttccgtccc gctcgtatcg ggcaacttcc tgaccgcccg
    10441 tccgataggt gtgattgacg gaaccgatat ggaatacgcg ggcgttatcc gcaaaaccga
    10501 caccgccgcc ctccgtttcc aactcgacgc gggcaatatc gtctggctgc cgccgctcgg
    10561 acattcctac agcggcaaga ccttctatct cgatatgctt caaaccgccg cctccgtcgc
    10621 cgtctcgctt caggccgaaa aactcgttta cctgaccctt tcagacggca tttcccgccc
    10681 cgacggcacg ctcgccgaaa ccctctcggc acaggaagcg caatcgctgg cggaacacgc
    10741 cggcggcgaa acgcgacggc tgatttcgtc caccgttgcc gcgctcgaag gcggcgtgca
    10801 tcgcgtccaa atcctcaacg gtgcctccga cggcagcctg ctgcaagaac tcttcacccg
    10861 caacggcatc ggcacatcca ttgccaaaga agccttcgtc tccatccggc aggcgcacag
    10921 cggcgacatc ccgcacatcg ccgccctcat ccgcccgctg gaagaacagg gcatcctgct
    10981 gcaccgcagc cgcgaatacc tcgaaaacca catttccgaa ttttccatcc tcgaacacga
    11041 cggcaacctg tacggttgcg ccgccctgaa aacctttgcc gaagccgatt gcggcgaaat
    11101 ctcctgcctt gccgtctcgc cgcaggcaca ggacggcggc tacggcgaac gcctgcttgc
    11161 ccacattatc gataaggcgc gcagtatggg cataagcagg ctgttcgccc tctccacaaa
    11221 taccggcgaa tggtttgccg aacgcggctt tcagacggca tcggaagacg agttgcccga
    11281 aacgcggcgc aaagactacc gcagcaacgg acgaaactcg catattctgg tacgtcgcct
    11341 gcaccgctga ccgcaacgga aagccgccgc agaaatgccg tctgaacccc gtttcagacg
    11401 gcatttcccc gattataaag acctgcccaa ccctcaagga ccccgatgaa atcctacccc
    11461 gacccctacc gccattttga aaacctcgat tccgccgaaa cgcaaaactt cgctgctgaa
    11521 gcgaatgccg aaacgcgcgc gcgtttttta aacaacgaca aggcgcgcgc gctttcagac
    11581 ggcattttga atcaaatgca ggacacgcgg cagattccgt tttgtcagga acaccgcgcg
    11641 cggatgtacc atttccatca ggacgcggaa tatccgaagg gcgtgtaccg cgtgtgcacg
    11701 gcggcgacct accgttcggg ctatccggag tggaaaatcc tgttttcagt ggcggacttc
    11761 gacgaattgc tcggcgacga tgtatatttg ggcggcgtgt cgcacttggt ggaaaagccc
    11821 aaccgcgcgt tgttgacact aagcaaatca ggcggcgata cggcgtacac gctggaagtg
    11881 gatttggaag caagggaatt ggtagaaggt ggttttcact ttccggcagg caaaaaccaa
    11941 gtgtcgtggc gcgatgaaaa cagcgtgtgg gtgtgtccgg cttgggacga acgccagttg
    12001 accgaatcgg gctatccgcg tgaggtgtgg ctggtggaac gcggcaagag tttcgaggaa
    12061 agcctgcctg tgtatcaaat cgataaagat gcaatgatgg tgaacgcgtg gcgttatctc
    12121 gatccgcagg gttcgccgat tgatttgatt gaagcgtcgg acggttttta caccaaaacc
    12181 tatttgcagg tgtcggccga aggcgaggcg aaaccgttaa acctgcccaa cgattgcgat
    12241 gtggtcggct atctggcggg gcatcttttg ctgacgctgc gcaaggactg gaaccgcgcg
    12301 aaccaaagct atccgagcgg tgcgctggtg gcggtgaagc tgaatcgggg tgaactcggg
    12361 gcggcgcagc ttttgtttgc gcctgatgaa acgcaggcat tggaaagcgt ggaaacgacc
    12421 aagcgttttg tggtggcgag cctgctggaa aacgtacaag gccgtctgaa agcgtggcgt
    12481 tttgccgacg gcaaatggca ggaagtcgaa ctgccgcgcc tgccttcggg cgcgttggaa
    12541 atgaccgacc agccttgggg cggcgacgtg gtttaccttg ccgccggcga tttcaccaca
    12601 ccgctgacgc tgtttgcgct ggatttgaac gtgatggaac tgaccgtcat gcgccgccag
    12661 ccgcagcagt tcgattcaga cggcatcgaa gtgcggcagt tttgggtgat gtcgtccgac
    12721 ggcgaacgca tcccttattt ccacgtcggc aaaaacgccg cgcccgacac gccgacgctg
    12781 gtttatgcct acggcggttt cggcgtaccc gagctgccgc attatctggg cagcatcggc
    12841 aaatactggc tggaagaggg caacgccttc gtgttggcga acatacgcgg cggcggcgag
    12901 ttcggcccgc gctggcatca ggcggcgcag ggaatcagca aacacaaaag cgtcgatgat
    12961 ttgcttgccg tcgtgcgcga tttgtccgag cgcggcatca gttcgcccca acacatcggt
    13021 ttgcagggcg gcagcaacgg cggcctgatt accgccgccg ccttcgtgcg cgaaccgcaa
    13081 agcataggcg cactggtgtg cgaagtgccg ctgaccgaca tgatccgtta tccgctgctc
    13141 tccgccggtt caagctggac agacgaatac ggcaatccgc aaaaatacga agtctgcaaa
    13201 cgccggttgg gcgaattgtc gccgtatcac aatctttcag acggcatcga ttatccgccc
    13261 gcgctcatca ccaccagcct cagcgacgac cgcgtccatc ccgcccacgc gctcaagttt
    13321 tacgccaaac tgcgcgaaac ctcgccgcaa tcttggctct actcgcctga cggcggcggc
    13381 cataccggca acggcacgca gcgcgaagcc gccgacgaac tcgcctgcgt gttgctgttt
    13441 ttgaaagagt ttttgggcta agggcgggga gcggcactgc cgccgcgaat gaaaaaaggt
    13501 cgtctgaaac tgctttttca gacgaccttt tttaatggtt gtttcaaatc aaaatatcta
    13561 tgccgccggc cccatcagca cttcttcaca tccgaaggca aaaatccgta atgccgtctg
    13621 aacgcttcgt tgaaccgtcc cgcgtggcgg tagccgcaaa agtgcatggc ggcttggacg
    13681 gtgctgccgg attcgatgag ggcgagcgcg tgttccagcc gcaggcggcg caggcatccg
    13741 gcgacggttt cgccggtttg cgctttgaaa tagcgtttca ggtagcattc gttcagtccg
    13801 acgcggcggg cgatttcggc gatggtcagc ggacgggcga attcgtgttg caggatgtcg
    13861 gcggcttcgt ctatgcgccg acggcggtaa ccgttgtcgt ggcggcggaa ggtgaagcgc
    13921 aataatcggg cggagagttc cagcgcggcg gcttcgtcgg caagcaggcc gaagccgtcc
    13981 gattcgaacg ggcgttgcag cagtgggcag gccgccgccg tcagtgccgc cgcgttttgc
    14041 gccagccgtt gcagggcgaa tcggcctatc gtttgcggcg aaaacaggcg ttcgtccagc
    14101 aagccttcgt cgtgccagcg gcgcagtttt tccagcgaaa aatccaaatg cagcgcgcac
    14161 atgccgctgt tgtcgggcag cagggtttcg gatacgtccg ccaactcgcc gcgtaccagc
    14221 cagatttcgc cggcagacgg gcggtattcc ctgccgccca tttgtaaccg gttctgcccc
    14281 gacaccatga cgaacaaggc gcagttgtgg ctgaaattgt ggatttcggt gggaaacgcg
    14341 cccgttccgc cgccgcgcat ccgcgacaag gtgatgcccg agtcgaagcg gttgatgcac
    14401 atttccagat gcaaaccggg ctgttttgcc tgcgcaatga gcgcgctgtc ggaacagccg
    14461 tccaacgccc agccggattt atcggagcgg acataggttt ggtactggcg gtagatggcg
    14521 gcggtgttca tgattggata ggaacgagtt gtctaacaaa tgaattaaat aggaattatt
    14581 accaataatc aagcgcaggg attggttgaa acggaaaagg tcgtctgaaa gggtgtttca
    14641 gacgaccttt tccgtatcgg gaatttgttt tgccgtatcg ggaattttgc gttttgcggc
    14701 gtggtttctg caggttgttt gcttaataat aaacattctt attcgtatgc aaaggaaccg
    14761 cacaccgtga aaccgcgttt ttattgggca gcctgcgccg tcctgctgac cgcctgttcg
    14821 cccgaacctg ccgccgaaaa aactgtatcc gccgcatccg catctgccgc cacactgacc
    14881 gtgccgaccg cgcggggcga tgccgttgtg ccgaagaatc ccgaacgcgt cgccgtgtac
    14941 gactgggcgg cgttggatac gctgaccgaa ttgggcgtga atgtgggcgc aaccaccgcg
    15001 ccggtgcgcg tggattattt gcagcctgca tttgacaagg cggcaacggt ggggacgctg
    15061 ttcgagcccg attacgaagc cctgcaccgc tacaatcctc agcttgtcat taccggcggg
    15121 ccgggcgcgg aagcatatga acagttggcg aaaaacgcga ccaccataga tctgacggtg
    15181 gacaacggca atatccgcac cagcggcgaa aagcagatgg agaccttggc gcggattttc
    15241 ggcaaggaag cgcgcgcggc ggaattgaag gcgcagattg acgcgctgtt cgcccaaacg
    15301 cgcgaagccg ccaaaggcaa aggacgcggg ctggtgctgt cggttacggg caacaaggtg
    15361 tccgccttcg gcacgcagtc gcggttggca agttggatac acggcgacat cggcctaccg
    15421 cctgtagacg aatctttacg caacgagggg cacgggcagc ctgtttcctt cgaatacatc
    15481 aaagagaaaa accccgattg gattttcatc atcgaccgta ccgccgccat cgggcaggaa
    15541 gggccggcgg ctgtcgaagt attggataac gcgctggtac gcggcacgaa cgcttggaag
    15601 cgcaagcaaa tcatcgtcat gcctgccgcg aactacattg tcgcgggcgg ctcgcggcag
    15661 ttgattcagg cggcggagca gttgaaggag gcgtttgaaa aggcagaacc cgttgcggcg
    15721 gggaaagagt agggagtcgt ctgaaaacgg agtttccgaa ggaagcgggg ggtttctgcg
    15781 aagctaaagt gcggtttcaa cgaattgaaa agcagcctgt atgttgaaaa taccgctcaa
    15841 gcaaacctac ggtttgccgc cctctcccta gccctctccc acagggagag gggattgggg
    15901 tgcaggctgc ctttaaggtt taggcaaatt tttaactt
//
LOCUS       DAWFLT010000040        15876 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWFLT010000040 DAWFLT010000000
VERSION     DAWFLT010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15876
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.040"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            101..220
                     /locus_tag="WGR74_001253"
     CDS             101..220
                     /locus_tag="WGR74_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ0905677.1"
                     /translation="MQRSHKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            233..1096
                     /locus_tag="WGR74_001254"
     CDS             233..1096
                     /locus_tag="WGR74_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HEZ0905678.1"
                     /translation="MENVETHSQNHRKRLIGTFFLVGLENLLMLVYPVFGGWAINAVI
                     AGQAWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAVT
                     ARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLPR
                     FAAISENLYFRLKNSLERDNHFIRKGDERQLDRHYGLLARLRVLISNREAFGYLCVGT
                     AMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQRI
                     EWSKRNIKAGT"
     gene            complement(1147..2199)
                     /gene="sohB"
                     /locus_tag="WGR74_001255"
     CDS             complement(1147..2199)
                     /gene="sohB"
                     /locus_tag="WGR74_001255"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ0905679.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSSETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(2303..2392)
                     /locus_tag="WGR74_001256"
     tRNA            complement(2303..2392)
                     /locus_tag="WGR74_001256"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2356..2358),aa:Leu,seq:taa)
     gene            complement(2495..2568)
                     /locus_tag="WGR74_001257"
     tRNA            complement(2495..2568)
                     /locus_tag="WGR74_001257"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2534..2536),aa:Cys,seq:gca)
     gene            complement(2594..2669)
                     /locus_tag="WGR74_001258"
     tRNA            complement(2594..2669)
                     /locus_tag="WGR74_001258"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2634..2636),aa:Gly,seq:gcc)
     gene            complement(2691..2766)
                     /locus_tag="WGR74_001259"
     tRNA            complement(2691..2766)
                     /locus_tag="WGR74_001259"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2731..2733),aa:Gly,seq:gcc)
     gene            complement(2790..2865)
                     /locus_tag="WGR74_001260"
     tRNA            complement(2790..2865)
                     /locus_tag="WGR74_001260"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2830..2832),aa:Gly,seq:gcc)
     gene            complement(2891..2966)
                     /locus_tag="WGR74_001261"
     tRNA            complement(2891..2966)
                     /locus_tag="WGR74_001261"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2931..2933),aa:Gly,seq:gcc)
     gene            complement(3046..3609)
                     /gene="pgsA"
                     /locus_tag="WGR74_001262"
     CDS             complement(3046..3609)
                     /gene="pgsA"
                     /locus_tag="WGR74_001262"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ0905680.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            complement(3715..4138)
                     /locus_tag="WGR74_001263"
                     /pseudo
     CDS             complement(3715..4138)
                     /locus_tag="WGR74_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4178..4288)
                     /locus_tag="WGR74_001264"
                     /pseudo
     CDS             complement(4178..4288)
                     /locus_tag="WGR74_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4420..4755)
                     /locus_tag="WGR74_001265"
     CDS             complement(4420..4755)
                     /locus_tag="WGR74_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0905681.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            4884..5789
                     /gene="mtrA"
                     /locus_tag="WGR74_001266"
     CDS             4884..5789
                     /gene="mtrA"
                     /locus_tag="WGR74_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ0905682.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(5851..6339)
                     /locus_tag="WGR74_001267"
     CDS             complement(5851..6339)
                     /locus_tag="WGR74_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0905683.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            6486..7388
                     /locus_tag="WGR74_001268"
     CDS             6486..7388
                     /locus_tag="WGR74_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0905684.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPCWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(7429..8559)
                     /locus_tag="WGR74_001269"
     CDS             complement(7429..8559)
                     /locus_tag="WGR74_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0905685.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKVG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPSHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            8764..11388
                     /gene="alaS"
                     /locus_tag="WGR74_001270"
     CDS             8764..11388
                     /gene="alaS"
                     /locus_tag="WGR74_001270"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ0905686.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGDAYPELKEKQVQIEEALKNEESRFAQTLETGMALLENALAKGSKKLDGEI
                     IFKLYDTYGFPYDLTADICRERNIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVDELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGRHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKPLTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPEMLVSVESWLCQKLS"
     gene            11450..12181
                     /locus_tag="WGR74_001271"
                     /pseudo
     CDS             11450..12181
                     /locus_tag="WGR74_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12890..15301
                     /locus_tag="WGR74_001272"
     CDS             12890..15301
                     /locus_tag="WGR74_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0905687.1"
                     /translation="MEELPEYITRGVFDFMFGIEAKVRQIQEEANIQAAATIGRLHDA
                     LKEEGIYEEHELRLFITRLLFLFFADDSAVFRRNYLFQDFLENCKEADTLGDKLNQLF
                     EFLNTPDQKRSKTQSEKFKGFEYVNGGLFKERLRTFDFTAKQHRALIDCGNFDWRNIS
                     PEIFGTLFQSVMDAQERREAGAHYTEAANIDKVINGLFLENLRAEFEAVKALKRDKAK
                     KLAAFYQKIQNLQFLDPACGCGNFLIVAYDRIRALEDDIIAEALKDKADGLFDSPSVQ
                     CRLKQFHGIEIDEFAVLIARTAMWLKNHQCNIRTQIRFDGEVACHTLPLEDAAEIIHA
                     NSLRTPWQAADYIFGNPPFIGSTYQTKEQKNDLESICGHIKGYGLLDYVCNWYVKAAG
                     IMAQHPQVQTAFVSTNSICQGQQVEILWGSLLNQGIEIHFAHRTFQWTSQAAGKAAVH
                     CIIVGFRQKPPMPSEKTLYDYPDIKGEPEKHAVANINPYLIDAPDLIIAKRSRPIHCE
                     PDMVNGSKPTEGGNLILSTAEKDALIAAEPLAEQYIRPFIGADEFLNGKTRWCLWFHG
                     VSDVKRNHDLKQMPQVQARIQAVKTMREASSDKQTQKDAATPWLFQKIRQPSDGNYLI
                     IPSVSSESRRFIPIGYLSFETVVSNLAFILPNATLYHFGILSSTMHNAFMRTVAGRLK
                     SDYRYSNTVVYNNFPFPESCRLPSENDRPDPLRAAVEAAAQTVLDARGQYRREAQEAG
                     LPEPTLAELYAPDAGYTALDKAHATLDKAVDKAYGYKTGKNTDDEAERVAFLFELYRK
                     AAAIA"
BASE COUNT         4035 a         4304 c         3757 g         3780 t
ORIGIN      
        1 ggaggattag gggggtattg gggtaaaatt agtggatatt tgaaacgaaa acagccgaaa
       61 acctgtgttt gggtgtcagc tgtcgggagg gaaaggaatt ttgcaaaggt ctcacaaagt
      121 atttgccaca ttcggcgaca agcgcacaac ccggctgttt gccgatttgg atgcactggc
      181 tgaagtgatg gaaaaaacaa tctcggaaaa taaaaaatag gggggcaaat atgtggaaaa
      241 tgttgaaaca catagccaaa accaccgcaa gcgattgatt ggcacatttt ttctggtcgg
      301 actggaaaac cttttgatgc tggtgtatcc ggtgtttggc ggctgggcga ttaatgccgt
      361 gattgcgggg caggcgtggc aggcgttgct gtacgctttg gttgtgcttt tgatgtggct
      421 ggtcggtgcg gcgcggcgga ttgccgatac gcgcacgttt acgcggattt ataccgaaat
      481 cgccgtgccg gttgtgttgg aacagcggca gcggcaagtc ccgcattcgg cggtaactgc
      541 gcgggttgcc ctgtcgcgtg agtttgtcag cttttttgaa gaacacctgc cgattgccgc
      601 gacatccgtc gtatccatat tcggcgcgtg catcatgctg ctggtgctgg aattttgggt
      661 cggcgtgtcg gcggtgggca tacttgcgtt gtttttatgg cttttgccac gttttgccgc
      721 catcagcgaa aacctgtatt tccgcctgaa gaacagcttg gaacgcgaca accactttat
      781 ccgaaaaggc gacgagcggc agctggaccg ccattacgga ctgcttgcgc gcctgcgtgt
      841 gctgatttcc aaccgcgaag ccttcggcta tctctgcgtc ggcacggcga tgggtatttt
      901 gttcggcttt gcttttgtga tgatgacgct caaaggctac agcagcgcgg ggcatgtcta
      961 ttcggtcggc acttatctgt ggatgtttgc catgagtttg gacgacgtgc cgcgattggt
     1021 cgaacaatat tccaatttga aagacatcgg acaacggata gagtggtcga aacggaacat
     1081 caaagccgga acttgaaaaa tgccgtctga acacgcttca gacggcattt ccatccgttc
     1141 ggcaaactac atcacatccg cccgccggtt gacaagtttg gcaaacaact tttcaacaga
     1201 agcttccgcc tgcaaaccaa tgcgctggat caggctttgc ttctcctgat atttcacttc
     1261 gataacctgt ttgttttcaa acgctttcaa caacaaatca tcactggtcg aaatctcgtc
     1321 aatcaagttc aacgccaacg cctgccgacc gaaccaatgc tcgcccgttg ccacttcctc
     1381 aatatccaat tgagggcggt tctcgctgac aaactgcttg aacaactgat gcgtttcctc
     1441 cagttcctgt cggaatttct gtttgccctt ttccgtattt tcacccataa aagtaaccgt
     1501 gcgcttaaat tcgcccgccg tcatcacatc cacatcaata tcatgttttt tcaacaggcg
     1561 gtggatattc ggtacttccg ctacaacacc caccgaaccg acaatcgcaa acggagcgga
     1621 aacaatttta tccgccacac acgccatcat ataaccaccg ctcgccgcca ccttatcgac
     1681 ggcgacggtc agcggaatat tgcgttcgcg caaacgccta agctgcgaag ccgccaaacc
     1741 gtaaccgtga accacgccgc ccggactttc caatctaagc agaacctcat cttcaggctt
     1801 ggcaatcaaa agcaccgccg taatctcatg acgcaaggat tctacggcgt gtgcatacaa
     1861 atcgccgtca aaatccaaca caaaaaggcg ggatttttgc gtttcggaag atttctcccc
     1921 accctccttc aaacgctttt tctctgcttt ggcttccgcc ttttcctttt tcttttcctc
     1981 tttttcctga tgttttgcct cttccccgct taaaaagaat gcttcaaacg attgccgctg
     2041 ttttttataa ttttccgaaa aatccgtcag tacgacactg ccgctttccg actgtttctt
     2101 actctgtacg atagccaaca caatcagcgc aattgcgccg aacacggtaa gcagttcgag
     2161 caggaaaata ccgtaattca gtaaaatttc tttccacatt gattggattt cctcttgttc
     2221 aggcatgaac atgtcaatat tgtccatcac cgtccgacag ataaaaaaat aaccgcttgg
     2281 agcggcattg tcattttcag cttggtgccc ggagccggaa tcgaaccggc acgggatgtt
     2341 tagtcccgac ggattttaag tccgttgtgt ctacctattt caccacccgg gcatttgtga
     2401 aaggtggagg cgggggcgcg gattttaacc ggcctatatg aagattgcac tcctcatagc
     2461 ataaacactc tgccaccccg ccgtagtacg ataatggagg cgagagtcgg aatcgaaccg
     2521 gcgtagacgg atttgcaatc cgctgcataa ccactttgct atctcgccct aaaactggct
     2581 tatctaaaaa acttggagcg ggaaacgagt ctcgaactcg cgacctcaac cttggcaagg
     2641 ttgcgctcta ccaactgagc tattcccgcg cgttcaaaca tatcggtttt tggagcggaa
     2701 aacgagtctc gaactcgcga cctcaacctt ggcaaggttg cgctctacca actgagctat
     2761 tcccgcgttg atatgtttga aataaaactt ggagcgggaa acgagtctcg aactcgcgac
     2821 ctcaaccttg gcaaggttgc gctctaccaa ctgagctatt cccgcaatga ttgtgggaaa
     2881 atgaaatttt tggagcggga aacgagtctc gaactcgcga cctcaacctt ggcaaggttg
     2941 cgctctacca actgagctat tcccgcccga tttcattctc caacatcgaa gagacgcaat
     3001 tattatggat tctgtttttg ccgtcaagct atttttatgt ttttttcagg cgatttcttt
     3061 ccacgccatt ttcagataat acagcatcga ccagactgtc agcaaagatg cgataaacat
     3121 caatacattg ccgataacaa caagattaaa tccgtaaaaa tcgggaatat tcagcagcag
     3181 caggaaaatt gccagcattt gcgcggcggt tttgaactta ccgacggtgg cgacggcaac
     3241 gctgttcctt ttgcccattt gcgccatcca ttcgcgcaat gcggaaatgg taatttccct
     3301 gccgataatg atcatggcaa acaaaacata ggtccggtcg agtttgacca gtaaaagtag
     3361 cgatacggca accatcagct tgtcggcgac gggatcgagg aaggcgccga aatcggaagt
     3421 ctgtttccac agtcttgcca aaaatccgtc aaaccagtcg gttaaggcgg caacggcaaa
     3481 aatgacggcg gcggtgagat taatcgtttc ctccgcgaac cacggaaaag gcaggtaaaa
     3541 aagggctgtc atgacaggaa tgagcaagac cctcaaccat gtgaggaaga tagggagatt
     3601 ccaaggcatc ggttttctct gtgcagactg taaagttgtg attataacgg ttatcctcat
     3661 aacccaaaac gtaaaattgc tgcatgggca ttcccccgcc ccgccaatct gttttcacat
     3721 tcttttcaaa cgcaggaaaa tggcgggcaa taaaagcaaa atacccagtt tcaggcagaa
     3781 aacggcaggt tgtgccaaca cttcgacaag gcggtcttcc gtgcgggcaa aatctttatt
     3841 gcttatagac actgccactg ttgcggtatt ccaacagaac gccgtttaaa aaacctttgc
     3901 cgacggtttc gcttaaaacg gctctaacct gctccgccct gatggttctg ccgatattgc
     3961 cgcctgtgca caaactgtcg aacccatagc aggaaagccg gtaatgctgc ccgtctgcat
     4021 ccagtttgat tgcccgtccg ctgcggttgg gggcggtaac ggtcaattcc gcatattcga
     4081 atgttttttc ttgttcgtga aatgccgtca ggtaaggtgc aataaaaacg gcggacaaca
     4141 gcagacagct tatggcggca aaccataccc agcgataata tagtggatta aatttaaacc
     4201 agtacagcgt tgcctcgcct tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt
     4261 cctgatttaa atttaatcca ctatatttca cgcttacccc ttgtttctca aatgccgtct
     4321 gaaataagcg gcttaatata ttgtttacag tattgggaag cataacagac aaaatgccgt
     4381 ctgaaatatt ttcagacggc atttcttatc cgaaacggat tatttttgcg tttcaaccgc
     4441 ttccaatgca cgcagggcgt aagtgtaagc ggcaccggca ttcagggcga tggcggttgc
     4501 caatgcgcct gcgatttcgc tgtcggtcgc accggctttg gcggcagcgg cggcgtgaac
     4561 gctgatgcag ctctcgcaac gagtggtaat ggcaacggcg atggcaatca gttcgcgcgt
     4621 tttggcatca agtgcttcgg cggcggcggc ctgttccaat gcgccgtagg cctgcagcat
     4681 tttaggatgc gccttaccca gctcgccgaa cgatttttta accaatgcgg tatgttcttt
     4741 ccaatcttta aacattttct tttcctttct cttgcgttta accctgatac gcgcttgcgt
     4801 atctgttttc gatgtgcgta ttattgcaat tattcagttg tgtttctcgt ttaatcatct
     4861 cattttatgg ttcaaaaaga tttatggaca ttctggacaa actggtcgat ttcgcccaat
     4921 tgacgggcag tgtggatgtg cagtgccttt tgggcggaca atggtcggta cggcatgaaa
     4981 ccttgcaacg cgaaggattg gtacacattg ttacatcggg cagcggctat ctctgcatcg
     5041 acggcgaaac ttccccgcgt ccggtcagta caggggatat tgtatttttc ccgcgcggct
     5101 tgggtcatgt gttgagccac gacggaaaat gcggagaaag tttacaaccg gatatgcggc
     5161 agcacggtgc gtttacggtc aagcagtgcg gcaacggaca ggatatgagc ctgttttgcg
     5221 cccgtttccg ctacgacacc cacgccgatt tgatgaacgg gctgcctgaa accgtttttc
     5281 tgaacattgc ccatccgagt ttacagtatg tggtttcaat gctgcaactg gaaagcaaaa
     5341 aacctttgac ggggacggtt tccatggtca acgcattgtc gtccgtcctg ctggtgctta
     5401 tcctgcgcgc ctatctcgaa caggataagg atgtcgaact ctcgggcgta ttgaaaggtt
     5461 ggcaggacaa acgtttggga catttaatcc aaaaggtgat agacaaaccg gaagacgaat
     5521 ggaatgtcga caaaatggtg gcggctgcca atatgtcgcg cgcgcaactg atgcgccgtt
     5581 tcaaaagccg ggtcggactc agcccgcacg cctttgtgaa ccatatccgc ctgcaaaaag
     5641 gcgcgttgct gctgaaaaaa aacccggatt cggttttgtc ggtcgcactg tcggtaggct
     5701 ttcagtcgga aacgcacttc ggcaaggcgt tcaaacggca atatcacgtt tcgccgggtc
     5761 aataccggaa agaaggcggg caaaaataaa tcggggcttc aaacgcaaat gccgtctgaa
     5821 aaggctttca tacagcattt gcgtaccgcg tcatttcaag ggctgcatct tcatcacttc
     5881 catcaaaaag ttggtaaatg cggggttgtt gggtttgaca tccatatttt tccaacgctg
     5941 ctgccagccg cgcaaggcat tctggatata cagcttggac tgttccgtat tgattgcgcc
     6001 ccgctggctg tctatcgccg aacgcaggta gatttcatac atactgtcat cgacggcatt
     6061 gcgtccgacc aggcgttttc tgaagttgtt cagatattgc gccgcctgaa ccttggtcat
     6121 tttaccgata cccacctgat agcccaagcg cgtcgcttca tcgctgattt tggcaacatc
     6181 cgtccaatgc gaagaggcaa ggcggaaacc ttttgcaggt gcttccgttt tgacggtatt
     6241 gataggattc acggggattt ccgtcaatgt gggcacataa atagactggc agccggaaag
     6301 aactgccaca atggaaagag ggataaggta ttttttcatg cccccattat aatcaagttt
     6361 gccttgagaa aacaaattgt tcggcaagaa aaataaaatt tcggcatcag aagcaggcaa
     6421 aaacacattc cacaagcctt gccgcaaggt ttacaatccg accgtcctta tcgcaacgac
     6481 cgtttatgga taccgcaaaa aaagacattt taggatcggg ctggatgctg gtggcggcgg
     6541 cctgctttac cattatgaac gtattgatta aagaggcatc ggcaaaattt gccctcggca
     6601 gcggcgaatt ggtcttttgg cgcatgctgt tttcaaccgt tgcgctcggg gctgccgccg
     6661 tattgcgtcg ggacaccttc cgcacgcccc attggaaaaa ccacttaaac cgcagtatgg
     6721 tcgggacggg ggcgatgctg ctgctgtttt acgcggtaac gcatctgcct ttggccaccg
     6781 gcgttaccct gagttacacc tcgtcgattt ttttggcggt attttccttc ctgattttga
     6841 aagaacggat ttccgtttac acgcaggcgg tgctgctcct tggttttgcc ggcgtggtat
     6901 tgctgcttaa tccctcgttc cgcagcggtc aggaaacggc ggcactcgcc gggctggcgg
     6961 gcggcgcgat gtccggctgg gcgtatttga aagtgcgcga actgtctttg gcgggcgaac
     7021 cctgctggcg cgtcgtgttt tacctttccg tgacaggtgt ggcgatgtcg tcggtttggg
     7081 cgacgctgac cggctggcac accctgtcct ttccatcggc agtttatctg tcatgcatcg
     7141 gcgtgtccgc gctgattgcc caactgtcga tgacgcgcgc ctacaaagtc ggcgacaaat
     7201 tcacggttgc ctcgctttcc tatatgaccg tcgttttttc cgccctgtct gccgcatttt
     7261 ttctgggcga agagcttttc tggcaggaaa tactcggtat gtgcatcatc atcctcagcg
     7321 gcattttgag cagcatccgc cccactgcct tcaaacagcg gctgcaatcc ctgttccgcc
     7381 aaagataaaa aatgccgtcc gaacatcctt cagacggcat atcgggcttt atttccccgc
     7441 cttcacatcc tgccactggc gcaccataaa cttcaatgcc gccggctgga taggcaccat
     7501 gataaagctg tttttcaaat cctcctcggt tgggaaaatc gtattgtcgt ttttaaattc
     7561 gtcttccatc agctcacgcg ccggcttgct cgaaggcgca taagtaacga aattgccgtt
     7621 tttcgccgac acttccgggt cgaggaagtc gttgatgtat ttgtgcgcgt tggcgacgtt
     7681 tttcgcatct ttcggaatca cgaaagaatc cacccaaatc cccacgccct ctttgggcat
     7741 catcacgcgg attttttcct tgccgcccgc ttcttcggca cggcgtttgg cgatgttcaa
     7801 atcgccgccg aaaccgattg ttacgcaggt atcgccgcgc gccaaatcat cgataaagcc
     7861 ggacgaagta aagcgtttga tattggggcg gtttttcttg agcagggcgg ttgcctccct
     7921 gatgtcttcc gtattgctgc tgttcgggtt tttacccaaa tagttcaaca ccataggata
     7981 gatttccgcc gcgctgtcta aataactgat accgcattgt ttgagtttgg acgtgtattc
     8041 ggggtcgaac accaaatccc actggttgtc cggcagcttg tccgtaccca aagccttttt
     8101 cacgcgttcg gtattgatgg cgaaggtatt tgtcccccaa taaaacggca cggcgtattc
     8161 gtggctggga tcgaccccgt ccatcagcct catcatttcg ggattgaggt gtttataatt
     8221 gggaatcagc gatttatcga ttttctgata cgcacctacc ttaatctgcc tgcccacaaa
     8281 cgcattggac ggcgcgacaa tgtcgtaacc ggattttccg gtcagcacct tgctttccag
     8341 cgtttcatcg ctgtcgtaca catcataagt aaccttgatg ccgtttttct tttcaaaatc
     8401 ggcaacggtt tccggatcga cgtattccga ccagttgtaa attttcaata cgtttcggtt
     8461 ttccgccggc gccggttttt cggcaggcgg tttgtccgaa ccgccgcacg ctgcaagcag
     8521 caaagcagtc aggacggcga gtggcagatg tttggccatt atcattcctt gcatatcggg
     8581 ttggagaaag cggtcattat agccgatatt ggcaacaggg cttcagacgg cattcaaaat
     8641 cccgccacac ccttccgaaa accgccgctt ccatagctag aaacagggat ttgcggtaag
     8701 ataccgccgt tcgttttccc tgcttttacc atgacaagac atttgagaga cattgaaaaa
     8761 attatgaaaa cctccgaact gcgccaaaaa ttcctaaaat tttttgaaac caaaggccac
     8821 accgtcgtcc gctcttccag cctcgtgccg cacgacgacc cgaccctgct gtttaccaac
     8881 gcgggcatga accagtttaa agacgtattc ttaggtttcg acaaacgccc gtacagccgc
     8941 gccaccaccg cgcaaaaatg cgtacgcgca ggcggcaagc acaacgactt ggaaaacgtc
     9001 ggctacaccg cccgccacca caccttcttt gaaatgatgg gcaacttctc cttcggcgac
     9061 tacttcaagc gcgacgcgat ccacttcgct tgggaatttc tgacttcccc cgaatggctc
     9121 aatatcccca aagacaaact gttggcgacc gtttacgcgg aagacgacga agcctacaac
     9181 atctggttga acgaaatcgg tatgccgtcc gagcgcatcg tccgcatcgg cgacaacaaa
     9241 ggcgcgaaat acgcgtccga caacttctgg caaatgggcg acaccggccc ttgcggcccc
     9301 tgctccgaaa ttttctacga ccacggcgaa gaaatttggg gcggcattcc cggcagcccc
     9361 gaagaagacg gcgaccgctg gatcgaaatt tggaactgcg tattcatgca gttcaaccgc
     9421 gacgaacaag gcaatatgaa tccgctgccc aagccgtccg tcgataccgg catgggcttg
     9481 gaacgcatgg ccgccgttat gcagcatgtt cacagcaact acgaaatcga cttgttccaa
     9541 gacctgctca aagccgttgc ccgcgaaacc ggcgcagctt tcagcatgga agaaccaagc
     9601 ctaaaagtca tcgccgacca catccgctcc tgctcattcc tgattgcaga cggcgtattg
     9661 ccttccaacg aaggccgcgg ctacgtattg cgccgcatta tccgccgcgc cgtgcgccac
     9721 ggttacaaac tgggtcaaag caaaccgttc ttccacaaac tggtggccga tttggtcaaa
     9781 gagatgggcg atgcctaccc tgaattgaaa gaaaaacaag ttcaaatcga agaagcgttg
     9841 aaaaacgaag aaagccgttt tgcccaaact ctggaaaccg gtatggcttt gttggaaaac
     9901 gcgcttgcca aaggcagcaa aaaactcgac ggcgaaatca tcttcaaact ctacgatacc
     9961 tacggtttcc catacgactt gactgccgac atctgccgcg aacgcaatat cgaactggac
    10021 gaagcaggct tcgagcgcga aatggaagcc caacgcgcac gcgcacgcgc cgcacaaagc
    10081 ttcaaagcca acgcccaact gccttatgac ggtcaagaca ccgagtttaa aggttatagc
    10141 gaacgccaaa ccgaatccaa agtcctcgcc ctctacaaag acggcgagca agtcgacgaa
    10201 ttgaacgaag gcgacagcgg cgcagtcgtc atcgacttta ccccgttcta tgcagaatcc
    10261 ggcggccaag tcggcgatgt cggctatatc ttctcaggcg aaaaccgctt tgaagtacgc
    10321 gatacccaaa aaatcaaagc ggccgtattc ggtcaattcg gcgtacaaac ttcaggccgt
    10381 ctgaaagtcg gcgacagcgt taccgccaaa gtggacgacg aaatccgcaa tgccaatatg
    10441 cgcaaccaca gcgcaaccca cttgatgcac aaagccctgc gcgatgtatt gggcagacac
    10501 gtcgaacaaa aaggctcttt ggttaccgcc gaatccaccc gtttcgacat ttcccatccc
    10561 caagcggtaa ctgccgaaga aatcgccgaa gtcgaacgcc gcgtcaacga agccgttttg
    10621 gcgaacgttg ccgtcaacgc cgccattatg agcatggaag acgcgcaaaa aaccggcgcc
    10681 atgatgctct tcggcgaaaa atatggcgac gaagtacgcg tattgcaaat gggcggtttc
    10741 tctaccgaat tgtgcggcgg cacgcacgtt tcacgcaccg gcgacatcgg cctcttcaaa
    10801 atcatcagcg aaggcggtat tgccgcaggc gtgcgccgta tcgaagccat caccggcctg
    10861 aacgcactca aatgggcgca agagcaagag cgtttggtga aagacatcat tgctgaaacc
    10921 aaagcccaaa ccgaaaaaga cgtactggcg aaaatccaag caggcgcggc acacgccaaa
    10981 gcattggaaa aagaattggc acgcgccaaa gccgaactcg ccgtccacgc aggcgcaaaa
    11041 ctcttggacg acgcaaaaga cttgggcgca gccaaactcg ttgccgccca aatcgaagcc
    11101 gacgcagccg ccctgcgcga aatcgtaacc gacctgaccg gaaaatccga caacgccgtg
    11161 attcttttag cggcagtaaa cgacggcaaa gtctccctgt gcgccggcgt atccaaaccg
    11221 ttgaccggca aagtgaaagc aggcgatctg gttaaatttg cagccgaaca agtcggcggc
    11281 aaaggaggcg gccgtcccga cttggcgcag gccggcggca cggatgccgg caaattgccc
    11341 gaaatgctgg taagcgtgga aagctggctt tgtcagaaat taagttaaat ttaaatctta
    11401 ataagccatt tgagtcttgt tcaaatggct tatattttgg attgttagaa tgaatactaa
    11461 aacagagtta caaaaacttt tagaagagga tatatcaact ttaacagaaa cactaatttg
    11521 tgcagatgct cttcctccaa gatatgtacg tagcattgca actcctattg tccgtcgatg
    11581 gttaattgac aaacaattaa atattctcgc aaaagaaata ggtttaacta tagaattgcc
    11641 catccttgat acaagtttgg tatttgaaaa attgtcaacc ttagagaata aagttaattt
    11701 ttatatggct ggtggagttt atttaggcgg agaatttatt agctctatat atcattcatc
    11761 acaagaattt tctggtgaac caataatcta tgctgaacca aatataattt tatgtcctgc
    11821 tgaaaaattt ttaactttaa aacgagtttt ccataatgga aatattttta atatgaatca
    11881 gataattact tttttatcca ataaacaagg aggagtacat tttgataaaa attacgataa
    11941 atataaaact tgacaagttg ctatagaaaa agcagcaaat tttttaaaac tcggtaatcc
    12001 atataatgaa gataaattat ctcttagtga agaacatgat actattttag tagttttacc
    12061 attagaaaaa ggatatgaat ggaattgtct tgagatagaa gtactatccg ctgctcaatc
    12121 tctagcaaat atctattgca acaaagttcg cctcatagat ggtcatgttt ggaaggaata
    12181 aaagaaaaag catagttggt aatttttagt tacgtagggt gggcttcagc ccaccgtttt
    12241 ttcatttttg attgttgatt ggtgggctaa agcccaccct acggttacta aaatcccgtc
    12301 attacctcgc aggtgggaat ccggtttgtt cggtttcggt tttttaggga cagtgccgca
    12361 acattaaatt tccagattcc cactttcgca caaataacag cgcaaatagg gcaaagataa
    12421 ggatatgggt ttttttcttt gaatttacta tacaattcgc ccgtccccgt tatagaaacc
    12481 caagccgatg aaaaccctgc tccaactcca aaccgccgca caaaacttcg ccgcctacta
    12541 caaagaccaa accgacgaac gccgcgagaa agacaccttc tagaacgaat ttttcgccat
    12601 tttcggcatc gaccgcaaaa acgtcgccca cttcgaatac cccgtcaaag accctgccga
    12661 caacacccaa ttcgtcgata tattttggga aggcatcttc cttgccgaac acaaatccgc
    12721 caacaaaaac ctgaccaagg ccaaagagca ggcggaacgt tatttacagg aaatcgggcg
    12781 caccaagccc tccgcgctgc ccgaatatta cgccgtcagc gattttgccc atttccacct
    12841 ttaccgccgc gtacctgaag aaggcgcaga aaaccaatgg cagttccctt tggaagaatt
    12901 gcctgaatac atcacgcgcg gcgttttcga cttcatgttc ggcatcgaag ccaaagtccg
    12961 ccaaattcaa gaagaagcca acattcaagc ggcggcgacc atcggcaggc tgcacgacgc
    13021 gctcaaagaa gaaggcattt acgaagaaca cgagctgcgc ctcttcatca cgcgcctgct
    13081 tttcctcttt tttgccgacg acagcgccgt tttccggcgc aactaccttt tccaagactt
    13141 tttagaaaac tgcaaagaag ccgacacgct cggcgacaag ctcaatcaac tctttgaatt
    13201 tctcaacaca cccgaccaaa agcgcagcaa gacccaaagc gaaaaattta aaggtttcga
    13261 atacgtcaac ggcggtcttt tcaaagaacg cctgcgcact ttcgacttca ctgccaagca
    13321 gcaccgcgcc ttaatcgact gcggcaattt cgactggcgc aacatcagtc cagaaatctt
    13381 cggcacgctc ttccaatccg tcatggacgc gcaagagcgg cgcgaagcgg gcgcgcacta
    13441 caccgaagcc gccaatatcg acaaagtcat caacggcctt tttttagaaa acctgcgtgc
    13501 cgaatttgaa gccgtcaaag ccctcaaacg cgacaaagcc aaaaaactcg ccgccttcta
    13561 ccaaaaaatc caaaacctgc aattcctcga ccctgcctgc ggctgcggca acttccttat
    13621 cgtcgcctac gaccgcatcc gcgcccttga agacgacatc atcgccgaag ccctcaaaga
    13681 caaagcagac ggcctgttcg acagcccgtc cgtccaatgc cgtctgaaac agtttcacgg
    13741 catcgaaata gacgaatttg ccgtcctcat cgcccgcacc gccatgtggc tcaaaaacca
    13801 ccaatgcaac atccgcacac aaatccgctt cgacggcgaa gtcgcctgcc atacgctgcc
    13861 gctcgaagac gccgccgaaa tcatccacgc caacagcctc cgcacacctt ggcaggcggc
    13921 ggactacatc ttcggcaatc ccccctttat cggctcgacc taccaaacca aagagcagaa
    13981 aaacgacctc gaaagcatct gcggccatat caaaggctac ggcctgttgg attacgtctg
    14041 caactggtac gtcaaagccg caggcatcat ggcgcagcat ccccaagttc agacggcatt
    14101 tgtttccacc aattccatct gccaaggcca gcaggtcgaa atcctctggg gcagcctttt
    14161 aaaccaaggc atcgaaatcc actttgccca ccgcaccttc caatggacga gccaagccgc
    14221 aggcaaagcc gccgtccact gcatcatcgt cggcttccgc caaaagccgc caatgccgtc
    14281 tgaaaaaacc ctctacgact atcccgacat caaaggcgaa cccgaaaaac acgccgtagc
    14341 caacatcaat ccttatctga tcgatgcgcc cgatttgatt atcgccaagc gcagccgtcc
    14401 catacattgc gaacctgata tggtcaacgg aagcaaaccg accgaaggcg gcaaccttat
    14461 cctttcaacc gccgaaaaag atgccctgat tgccgccgaa cccttggcgg agcaatacat
    14521 ccgccccttt atcggcgcgg atgagtttct caacggcaaa acccgttggt gcctgtggtt
    14581 tcacggcgta tccgatgtca aacgcaacca cgacctgaaa caaatgcccc aagttcaagc
    14641 ccgtattcag gcggtcaaaa ccatgcgcga agccagcagc gacaaacaaa ctcaaaaaga
    14701 tgcagcaacc ccgtggcttt ttcaaaaaat ccgccagcct tcagacggca attatctgat
    14761 tattccgagc gtgtcgtctg aaagccgccg tttcatcccc atcggttatc tgtcgtttga
    14821 aacagttgtc agcaatctgg catttatcct tccaaacgcc accctctacc acttcggcat
    14881 cctcagctcc accatgcaca acgcctttat gcgtaccgtt gcaggtcgtc tgaaaagcga
    14941 ttatcgctac tctaataccg tcgtgtacaa caacttcccc ttccccgaaa gctgccggtt
    15001 gccgtctgaa aacgaccgcc ccgacccgct ccgcgccgcc gtcgaagccg ccgcccaaac
    15061 cgtcctcgac gcgcgcggac aataccgccg agaagcgcag gaagccggtt tgcccgagcc
    15121 gaccctcgcc gaactctatg cgcccgacgc aggctatacc gccctcgaca aagcccacgc
    15181 caccctcgac aaggcagtcg ataaagccta cggctacaaa acaggcaaaa ataccgacga
    15241 cgaggcagaa cgcgtcgcct tcctgttcga gctgtaccgc aaggcggcgg caattgcgta
    15301 gtagttgcgt agtagttgcg taggatagtt gcgtagggtg ggcttcagcc caccaactcc
    15361 caccaactcc caccatcttc accctaaaac cccgtcatct ccgcaaaagc aattgcgtag
    15421 ggtgggcttc agcccaccgt ttttcttcat tttcggttgt tgattggcag gctgaagccc
    15481 accctgcggt tacaaaaatc ccgtcattcc cgcgcaggcg ggaatccaga cctttcgggg
    15541 cggaaactta tcggataaaa cgatttcccc tgcatcctgg attcccactt tcatgggaat
    15601 gacgggatat aggtttccct acggacgtgt tcagattccc gccttcgcgg gaatgacggc
    15661 ggagaagttt ctgtctttcc gataacttac cgtaacttaa aatcccgtca ttaccgcaaa
    15721 agcagttgct acggttacaa aaatcccgtc atccccacga aagcgaaaat cctgcctgtc
    15781 ggatttcggt tttttcgggc gtttcgggaa cttataaatc gtcattcccg cgcaggcggg
    15841 aatccagaac gcgggacggc ggcaatattc aaaggt
//
LOCUS       DAWFLT010000041        15655 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWFLT010000041 DAWFLT010000000
VERSION     DAWFLT010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15655
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.041"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(16..771)
                     /locus_tag="WGR74_001273"
     CDS             complement(16..771)
                     /locus_tag="WGR74_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ0905688.1"
                     /translation="MKFTRLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGFKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCASR"
     gene            complement(951..2111)
                     /gene="nirK"
                     /locus_tag="WGR74_001274"
     CDS             complement(951..2111)
                     /gene="nirK"
                     /locus_tag="WGR74_001274"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HEZ0905689.1"
                     /translation="MKRQALAAIIASMFALAACGGEPAAQTPAASAEAASSAAQTAAE
                     TPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWTFDGD
                     VPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTSTFSFK
                     ALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKGKKGA
                     QGLQPFDMDKAIAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLVSSFH
                     VIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRAFNKG
                     ALGQLKVEGAENPEIMTQKLSDTAYAGNGAAPAASAPAASAPAASAPAKSDY"
     gene            2481..4736
                     /locus_tag="WGR74_001275"
     CDS             2481..4736
                     /locus_tag="WGR74_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ0905690.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSVVISDTRAKAIESILPYYHGVY
                     GDDPALQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPGEVFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLVFIGGAICVAI
                     QATKIVFGRDK"
     gene            complement(5000..5704)
                     /locus_tag="WGR74_001276"
     CDS             complement(5000..5704)
                     /locus_tag="WGR74_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0905691.1"
                     /translation="MDFLERLKSLWPDNAKPADFYNKIDMSASGFSRVWKDGAIPTAD
                     YLIKIQEVTGCDLNWLLTGQGSPYMNKGQAVEVRTHTDGTATDTLGNPINLDEFVFIP
                     RYDVYAAAGHGYPAEDDKPLFCMAFRRYWIENYVTRQLDKLSVIAVKGDSMEGVLNHG
                     DNILVNHAETTPRDGLYVIRIDNHLFVKQIQKLPGKLLVKSANPVYEPFEIDLTDDNQ
                     NVAIIGRVEWYGRTVN"
     gene            5921..6184
                     /locus_tag="WGR74_001277"
     CDS             5921..6184
                     /locus_tag="WGR74_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ0905692.1"
                     /translation="MQKNATPKNWHRADIVAALKKKGWSLRALSIEAGLSPNTLRSAL
                     AAPYLKGERIIAAAIGVEPEEIWPERYADRNLKPVFPKRVVNG"
     gene            6262..8307
                     /locus_tag="WGR74_001278"
     CDS             6262..8307
                     /locus_tag="WGR74_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0905693.1"
                     /translation="MLISAVELAGLQLPKLPNSRQGIEYHAKKNNWPFEEVVGQARGG
                     KLKKYLVSALPSEIQAAIQEKQAATLLAKVPMLPAEVKKPLRQNKKMRQLGLIPCEEG
                     LARLDDRQTETAHARCAIVAYVLPLHELAGMPIKKAVAFVAAEAAAGRLPEDVAKLIP
                     LANARNNGERSLSEPTLYRWVRAYRAASDSMSRLLALAPVKTREKTPLLAIDWLPYFL
                     MFYQRPNKPTMMAAAKKLAQWYLEQGKIEQMPSYDQIQTVMKRLPEHMKERGRRTGSA
                     YKALLPYIDRDWTALKPNDVWVGDGHSFKAKIRHPMGYLFTPEVTMIVDGCSGAVVGW
                     SVALSETAVAVADALRHGMTHFPPPLGYYSDNGSGETGDMLDKETTGILPRIGIEHFT
                     GIPGNPQGRGKIERLWQTITIPLAKQYATYQGKDADAETLRKVSNALASAQKVQKKGK
                     ELSKAQEAALSAAPTWAQFIADLEEAVRRYNFEHEHRSLPKNPEAGRHFTPMKYYEYR
                     METDGMRVKTDVLSPLELDFMYRPEEERIPDRGAVSLHNNTYFHQELLDYSGQKVRVA
                     YDIHDADHVIVKDMQGKVICKAVFNGNKRAAFAETRMEQLAERRRKGQAKRLQDKMDL
                     IEAQRQSAAPIIEQQPDFGEFLKLETESGGLVEVENRPSESGRGKKKLRDFLWEDAG"
     gene            8349..9263
                     /locus_tag="WGR74_001279"
     CDS             8349..9263
                     /locus_tag="WGR74_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0905694.1"
                     /translation="MIEKLRQFLETSGMSQNKAADRMGVSRSALSGYLNGKYDGDIAG
                     MDKKAALFLEQEGDRAELKKLDIPYVETGTAKKMKGWLGLAAWLGQLGIVYGGAGLGK
                     TTVLKQYAATNPLALLIEPDTGYTAKVLLQEICHALDLSERGNIHELTERIINCLKRD
                     KKSRSPRDAHRILLIDEAEQLPTRALESLRRIHDKSGVAVALVGMPKLLLNLKGPNSE
                     FKQLFSRVSVKMELGEMLPETDLKQIAAAVLKTNDEAVLQKVVKTAKGNARKLSKLLL
                     IVDYLLQVNPDVDLDDDVIEHAETYLIH"
     gene            9339..9581
                     /locus_tag="WGR74_001280"
     CDS             9339..9581
                     /locus_tag="WGR74_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905695.1"
                     /translation="MENLKMRHLLAIPAIVIAVTACANRCTEPVPAWSLYQNETSQQR
                     LMREKQNAADFAVLQAEKAYERMSDKERMKGVVYEN"
     gene            9571..9870
                     /locus_tag="WGR74_001281"
     CDS             9571..9870
                     /locus_tag="WGR74_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905696.1"
                     /translation="MKTETKICKKCGEERPLFRFKKDDAYAGGYRPVCYECLNARAKE
                     LKAVRDSMAERKIISKGISINGGKTHMEFPEEPEFMKNKYWTVTKTDYRRPLKGL"
     gene            9873..10025
                     /locus_tag="WGR74_001282"
     CDS             9873..10025
                     /locus_tag="WGR74_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905697.1"
                     /translation="MSKRLARRKMPKHELKRVKAFALKQAVEKIRARWGEKAIRLAST
                     DNKQTD"
     gene            10041..10205
                     /locus_tag="WGR74_001283"
     CDS             10041..10205
                     /locus_tag="WGR74_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905698.1"
                     /translation="MMNEMNAVAALLVIAILLAAVMTVLVEILVEQRVKERLGKLLDK
                     ACRPDDGWQE"
     gene            10186..10401
                     /locus_tag="WGR74_001284"
     CDS             10186..10401
                     /locus_tag="WGR74_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905699.1"
                     /translation="MTGGRNDKEGNVMNMVLTIYFWLVGLVCFARSAKETYYAESLYG
                     LLTGLLGVMCSAFVVSVSVARVMGWLK"
     gene            10398..10712
                     /locus_tag="WGR74_001285"
     CDS             10398..10712
                     /locus_tag="WGR74_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905700.1"
                     /translation="MNQLKMTIAKPETEDFEDAWAFIRMLNLVTYDLNPLKTDTDGEY
                     EYLADEDKSDVLDAVVEKFNECSLEWMLSALQALMSPEMGIINQDSDTLELHPKLKGG
                     TE"
     gene            10831..11208
                     /locus_tag="WGR74_001286"
     CDS             10831..11208
                     /locus_tag="WGR74_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905701.1"
                     /translation="MRYLTLFRPAEKDLSFNRVSKLVGEIAPMIRDGKIVRNRKTYPA
                     PREAWIWAATRCLEARDAGKLTPPLTSHGYLLENITFWSPEKTAGTAVLPSPQPSHTR
                     EVASTKLRSGLGGLMEWSNGGKQ"
     gene            11192..11647
                     /locus_tag="WGR74_001287"
     CDS             11192..11647
                     /locus_tag="WGR74_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905702.1"
                     /translation="MEENNSWLKKAIAQGFMMLAALNLKGRPASADLTAVAELWLVIL
                     SGRSWQPEHDGIRIQAAFRAIAASSSEWPNPADLIKHLPPGEVRMVPRLEKKHCPTEY
                     GKEQAAELKKIVGRLKNAPCMNRDWIHGQRHRSVDECKRIYAERQKGNK"
     gene            11619..11813
                     /locus_tag="WGR74_001288"
     CDS             11619..11813
                     /locus_tag="WGR74_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905703.1"
                     /translation="MPKGRREINSQMSTFFDNQESDLINEFLTEYWEEFEKFSANNGF
                     DDDLLKQISMELEGHRNHYE"
     gene            11806..12417
                     /locus_tag="WGR74_001289"
     CDS             11806..12417
                     /locus_tag="WGR74_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3164 family protein"
                     /protein_id="HEZ0905704.1"
                     /translation="MSNLDMKQYKQDAKGNLVPLANIKEIDLLRDELVQEIAAKARAV
                     QDNLMAFKREAMDDIAAFVQLSADRYDVSVGGKKGNISLHSFDGAYRVNLAIQDTLVF
                     DEGLIAAKALIDECINEWTEGSRTELKTLINAAFQVDKEGNISTARVLGLRRLQITDE
                     KWQRAMDALSDSLQVHISKPFVRVYQRGEDGEYQLMNLDVAKV"
     gene            12423..13073
                     /locus_tag="WGR74_001290"
     CDS             12423..13073
                     /locus_tag="WGR74_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905705.1"
                     /translation="MNANIMIYLIEVNDGPVAKALRSFQQDKAAMRQAWLDWAREHLP
                     SDALMREWSDGQVAGFAFPSGIPDGWKKPNKNGICWPRQNNPILKTMPLNKRFKRPEE
                     YLEEVGITAPTMIFEKNSDGETWASWGIGNFFNPVQFVWAGLEEDAPKGVVTPDYAYE
                     LREGAKRIRNGCTMQPPEDFDWQNLLPGCRVIPRYEWDYLVGKWQETRNDAEEQEA"
     gene            13074..13274
                     /locus_tag="WGR74_001291"
     CDS             13074..13274
                     /locus_tag="WGR74_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905706.1"
                     /translation="MAKIIIEIEDLPEGTDINFKGDLPAADAKDKTGAQQTAVLISKM
                     IQAAQMMASPSVNIRPSETANP"
     gene            13290..13598
                     /locus_tag="WGR74_001292"
     CDS             13290..13598
                     /locus_tag="WGR74_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ0905707.1"
                     /translation="MPHLNLNRSQKVNKSELIQAIADEAELSKRAAAEFVNAFVSVVT
                     QELKDGNDVTLVGFGTFHAAQSAERQGRNPKTGEPLTIAARKTPKFRSGKALKDAVNG
                     "
     gene            13674..14108
                     /locus_tag="WGR74_001293"
     CDS             13674..14108
                     /locus_tag="WGR74_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1018 domain-containing protein"
                     /protein_id="HEZ0905708.1"
                     /translation="MMNTNEQYKRKGLIAKIKIAQSQLGMEDDVYRAMLARVCGKTSC
                     TQMNLAELQAVAAEMKRMGFKQTAPKGKGIRPHLTQDRAALLNKLEALLTVGDKSWQY
                     ADGMAKRMFGKDLVRFLTPEQLYKLVQALQIHINKAKKAAEQ"
     gene            14105..14551
                     /locus_tag="WGR74_001294"
     CDS             14105..14551
                     /locus_tag="WGR74_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905709.1"
                     /translation="MIRVDEEDFEAVRHLLPESVLALITIIGLTETVELVKNLGGTTY
                     PLRQGRTKGSESRLAYLEEIVGGAAMEKMVEALAPCDLFIPKCEQALLELRDRHIRRR
                     FDAQTGKGVPAYEAVNDLALAHALTDRHIWRILKKPDNECEQGGLF"
     gene            14599..15240
                     /locus_tag="WGR74_001295"
     CDS             14599..15240
                     /locus_tag="WGR74_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905710.1"
                     /translation="MKTFYVLMLAAMLAACGGQENKPAAGESQTDNRAQQYQNQQKQI
                     AARLADEEYFIGENNLEQIRLHANLKERAQKLLSLLAQADKESRVWVLPGDAAKIKEF
                     NAAFAAVAKSAEESFGGPFLADKAGLYQCTAAANAAYDYFFARQRKDALTENYRQQYT
                     DNIAACQGQIRTPPAAQATVYARKGIRLPLNNCLPVLAGDEEFDTYTCPMEVK"
     gene            15372..>15655
                     /locus_tag="WGR74_001296"
     CDS             15372..>15655
                     /locus_tag="WGR74_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0905711.1"
                     /translation="MSKIIVLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTV"
BASE COUNT         4040 a         3457 c         4491 g         3667 t
ORIGIN      
        1 ctataattga aggggttatc ggcttgcgca acggaagccc aagttgtgca agacatattt
       61 ggattgcagg ctggtgcgga tgccgtagcg gaggaaggcg gcatagttgg acgagtcgct
      121 cgacccaata gacgcgccgc tgcaaaacat ttgcgcattg gcatttccgg aggaaagcag
      181 gctgctgttg aaatcttccg tccattccca aataagtccg tgcatatcat aaacgcccca
      241 gtagttggga cggcccttgc cgacgtcatg caagcctttt cgtccgccgt cggcatacca
      301 atcgagaata gtgcggttgt agccgggttc gtttgagccg tttttctgcg tggcggaagc
      361 aagtccggca aattcccatt cgtcaatggt cggcaggcgt ttgccttgtg cggcgcaata
      421 ggcgttggcg gcaaaccagg aaacattggt taccggttgt tttaattcgc ccgccttcgg
      481 cgcatagctg cggctgccgt ttttcatcca atgcttcagg taagcgggtt ctgcctgttt
      541 gaaaccgatc ctgccttttt gccattgggg gtggctgttg acaaattcgg caaactcggc
      601 attggtaacg ggatatttat ccagtttgaa cggtttgact ttaatcaggc cggtatcttt
      661 tttcagataa agcgggcggt agctgccgcc ttcgatttga accatttcgg cagctgccgc
      721 ttgagtgccg gcgagtgccg cacagagaaa gagtaaccgg gtaaacttca tatcatgcct
      781 cctgacaggc agttaaaatt gatttttcag acggcatttt aaacaaaaag ccgcgaatgc
      841 cgtctgaaga atcaatcttc cgaaaggaaa gattggttgt taaaaaacca ctgccgtgcg
      901 taattaagta cggcggcagt ggttcgtttg cctaaaaatg ggtgttcaat ttagtaatcg
      961 ctttttgcag gagcagaggc tgcaggagca gaagctgcgg gagcggaagc agcaggagct
     1021 gcaccgttac cggcgtaagc ggtatcactc aatttttgag tcatgatttc agggttttct
     1081 gcaccctcta ctttcaattg acccagtgcg cctttgttga atgcgcggaa gatagagtgg
     1141 tcaaccaaag tgtagctgcc cgggatgtcg actttgaatt cgacgatggc agagccgccg
     1201 gcaggaacga tggtgctttg tacgttttcg ttaatcagtt tgccgccttc aacataaact
     1261 ttgtcgaaga tttcaccgat tacgtggaag gaagacacca agttaggacc accgttacca
     1321 acgtacatac gtacagtttc gcctgctttg gctttcaggg cgttgtcgcc ggcgatagca
     1381 cctacgtgac cgttaaatac aacgtattca ggttgttcgg cgatggcttt gtccatatcg
     1441 aacggttgca ggccttgcgc gccttttttg cctttggtgt agaagtcgcc ttggacgatg
     1501 tagaactctt tatccacttt cggcagacct tctttaggct cgaccaaaat cagaccgtac
     1561 ataccgttgg cgatgtgcat accgaccggc gcgacggcgc agtggtagat gtacagaccc
     1621 ggttgcaggg ctttgaagct gaatgtggaa gtacggcccg gagcggtaaa ggttgcggcc
     1681 gcgccgccac cttggccggt agctgcatgg aagtcaacgt tatggggaac ggtagaagaa
     1741 ggattgttag agaattctac ttctacggta tcaccttcgc gtacgcgaat catacggccc
     1801 ggaacgtcgc cgtcaaatgt ccagtagcgg tattccaccc cgtcgtccat tttcatggtt
     1861 ttttcgacgg tttccatttt tacgcgtact ttggcaggat agtcgcggtc gattgcggga
     1921 ggtacttcag gagcgtgggt ggtaactgca tcgatgacgg gcagttcgcc tgcaggcgtt
     1981 tcggcggcgg tttgtgcggc ggagcttgcg gcttcggcgg aagcggcagg ggtttgcgcg
     2041 gcaggttcgc caccgcaggc ggctaaagca aacatagaag cgataattgc ggctaaggct
     2101 tggcgtttca taatgttttc cttttgtaag aaaagtaggg agattggtta agaactggtg
     2161 tgattatgcg tttgccccat caccgacatt ttgatttaag tcaaataatt cataaaatat
     2221 gaacttaaaa tgatacttct aatactttat agctactaaa aattacaata aataggctga
     2281 tttgcttaag ggcttcggca aatcaggcaa aggcagggta gttgccgact aaagaataat
     2341 gatagttatt atcatatatg tatttgtttt atatgtgagt ttctttgaac aaatgtttat
     2401 ttgcgcggta aaccgtgcta caatctttaa cattcatatt ttgtgaattt taatccacta
     2461 tattaaaagg agctctcaaa atgggacagt acaagaagct gtggtacttg ctgtttgccg
     2521 ttctggcggt atgctttacc attcttggct atatgggcag cgaggtttat aagaaagccc
     2581 cgccttaccc cgaacaggtc gtttccgcat caggcaaagt gctgatgacg aaagacgata
     2641 ttttggcagg tcagtctgcg tggcagagta ccggcggtat ggaagtcggt tcgattctgg
     2701 gtcacggcgc atatcaggct ccggactgga cggcggactg gctgcatcgt gagttgtccg
     2761 cttggttgga tttgaccgcg caacagactt acggcaaaaa attcgatgaa gtttcccctg
     2821 aagaacaagc cgttctgaaa acccgactgg ctgacgaata ccgcaaccaa agccgtatta
     2881 aagaagacgg cagcgtcgtc atcagcgata cgcgcgcgaa agccatcgaa agcatcctgc
     2941 cttattacca cggtgtttac ggcgacgatc ccgcgctgca aaccacccgc gaacactttg
     3001 caatgaaaaa caacacattg ccaagtcagg aagcgcgtga aaaactgttc gacttcttct
     3061 tctggacttc ttggtctgct tcgaccaacc gtcccggcga ggtctttacc tacaccaaca
     3121 actggccgca cgagcctttg attaacaatg taccgactac tgaaaactac atgtggtcgt
     3181 ttaccagtgt tgtactgctc ttgatgggca tcggtttgtt gatgtggggt tattccttcc
     3241 tgaccaaaca cgaggaagtg gaagtgccgt ctgaagaccc gatttccaaa atccaactga
     3301 ccccttcgca aaaagcattg ggcaaatacg tcttcctgac ggttgccctg tttgtggtac
     3361 aagtattgct gggcggtctg actgcgcact acaccgttga aggtcagggt ttctacggca
     3421 ttgacgaggc gttgggcttt gaaatgtccg actggttccc ctacgccctg acccgtactt
     3481 ggcacatcca atccgccatc ttctggattg caaccggctt tttgacggcg ggtttgttcc
     3541 tcgcgccgat tgtcaacggc ggcaaagatc ccaagttcca acgtgcaggc gtaaacttcc
     3601 tctatatcgc cctcttcatc gtggtcggcg gttcttacgc gggcaacttc ttcgcgttga
     3661 cgcacatcct tccgccggaa tttaacttct ggttcggaca ccaaggttac gaatacctcg
     3721 atttgggacg tttctggcaa ctcctgctga tggtcggcct gctgttgtgg ctgttcctga
     3781 tgttgcgctg cacggtttcc gccttcaaag aaaaaggcgt ggacaaaaac ctgctggcaa
     3841 tctttgtcgc ttctatggtc ggtgtgggcg tattctacgc accaggcctg ttctatggcg
     3901 aaaaatcccc gattgccgta atggaatact ggcgttggtg ggtggttcac ctgtgggtgg
     3961 aaggcttctt tgaagtattt gccactgccg cttttgcctt tgtcttctac aatatgggct
     4021 ttgtccgccg cagtaccgcc acggcctcta ctctggccgc tgccgccatc tttatgttgg
     4081 gcggcgttcc gggtacgctg caccacctgt acttctccgg ctctacctcc gcctctatgg
     4141 caatcggtgc ctgcttctcc gctttggaag tcgtgccgct ggtgttgctg ggccgtgaag
     4201 catacgagca ttggtcttac caacacctgt ccgactgggc gaaacgcctg cgttggccgc
     4261 tgatgtgctt cgtggcagtc gccttttgga atatgatcgg tgccggtgta ttcggtttcc
     4321 tgattaaccc gccgatttcc ctgttctata tccaaggttt gaatacttct gctgttcatg
     4381 cgcacgccgc cttgttcggt gtgtacggtt tcttggcatt gggcttcgta ttgctggttg
     4441 cccgttattt gaaaccgaac gcgcagtttg acgacaaact gatgacttgg ggcttctggc
     4501 tgctcaacgg cggcttggtc ggtatgattg ccatcagcct gttgccggtt ggcgtgattc
     4561 aggcatacgc ctccatcacg cacggtctgt ggtatgcccg cagcgaagag ttcctgcaaa
     4621 tggaaatcct cgataccttg cgttgggtac gtacggcagc tgacttggtc tttatcggcg
     4681 gtgcaatctg cgttgccatc caagctacta aaatcgtatt cggccgcgac aaataagttt
     4741 tgcggtatga aatgagcaac ttatggggta ggtattattt tgacaccttt agaaagaaag
     4801 caaaagaagg cgaaaatttt aaaggaaaca aaaatttgaa cggaaattta ggaaaaagcg
     4861 gctttgacac ctttaggtgc aaagtaggta gaaagtgata taactttgat accttttttg
     4921 cacggttttg atgaggtttt gggttgattt tggttttatt tgaattgaac ggcgtttgga
     4981 atatccaaac gccgtttttt tagttgacgg tacggccata ccattctacg cgtccgatga
     5041 tggctacatt ttggttgtcg tcggttaaat ctatttcgaa aggctcataa accggattgg
     5101 ctgatttgac gagcagcttg ccgggtagtt tttggatttg tttgacaaac aggtgattat
     5161 cgatgcggat aacgtacagg ccgtcgcgcg gggtggtttc ggcgtgattc actaggatgt
     5221 tgtcgccgtg gttgagtacg ccctccatcg aatcgccctt tacggcgatg acggacagtt
     5281 tgtcaagctg gcgggtaacg tagttttcta tccaataacg cctgaatgcc atgcagaata
     5341 agggcttgtc gtcttctgcg ggatagccgt gccctgcggc ggcgtagacg tcgtaacgcg
     5401 ggataaagac gaactcgtct aagttgatgg ggttgccgag ggtatcggta gccgtaccgt
     5461 cggtatgggt acggacttcg accgcttggc ctttattcat gtatggcgag ccttggccgg
     5521 taagaagcca gtttaaatcg caaccggtta cttcttgaat tttaatcaga tagtctgctg
     5581 ttggaatggc accgtctttc cagacacggc taaaaccgga ggcagacata tcaatcttat
     5641 tgtagaagtc ggcgggtttg gcattgtccg gccacaaaga tttcaaacgc tctaaaaagt
     5701 ccatgattgt ccttttgctt agaatttcgc atttaagcaa aaaatacact tcttttgctt
     5761 aaaattaatt cgtcaaataa tcaaatagat ggctaatatt ttgctttttt gcgttatatt
     5821 tctaagcaaa agagttgcaa taaccttttt tgcttagtat tattcactca cttaatcaat
     5881 taacgaagta aatcacatta ctgaatggaa taagtcaatt atgcaaaaaa acgcaacgcc
     5941 gaaaaactgg caccgcgcgg atattgttgc tgctttgaaa aagaaaggct ggtcgcttcg
     6001 agcactttca atagaagcgg ggttgtcgcc gaatacgctt agaagcgcac tggccgcccc
     6061 ttatcttaag ggagaaagga ttattgccgc tgcaatcgga gtggaaccgg aagagatttg
     6121 gcccgaacgg tatgcagatc ggaatttaaa acctgttttc cccaaaagag tagttaatgg
     6181 ataagtggtt ttcgatttag ttccttaaat gctaaaggtt atacgcattt aagtaaaaca
     6241 caaaaagaaa gaaatagcaa aatgttgatt tcagcggttg agttggcggg cttgcaactt
     6301 ccaaagctgc cgaacagtag acaaggcatc gagtaccacg ccaaaaagaa taattggccg
     6361 tttgaagagg ttgtaggaca ggctcgaggc ggcaagctta agaaatattt ggtttccgcc
     6421 ctcccttcgg agattcaggc ggccatacag gaaaaacagg cggcaacatt gttggccaag
     6481 gtgccgatgt tgccggcaga agtaaaaaaa ccgcttcggc aaaataaaaa gatgcggcag
     6541 ttgggtttga taccgtgcga ggaaggtttg gcgcggttgg acgacaggca gacggaaacg
     6601 gcccacgcgc gttgtgcgat tgtcgcctat gtattgccgc tgcatgaatt ggcgggtatg
     6661 ccgattaaga aggcggtggc gtttgtggca gctgaagcgg cagcaggcag gttgccggag
     6721 gatgtggcca agctgattcc gttggcgaat gcgcggaaca acggcgaacg cagtttaagc
     6781 gaaccgactt tataccgatg ggtgcgggct taccgtgcgg catcggacag tatgagccgc
     6841 ctcttggcac ttgcgccggt gaaaacacgg gaaaagacgc cgctgctggc gattgactgg
     6901 ctgccttact tcttgatgtt ttatcagcgg ccgaacaagc cgacgatgat ggcggcggcg
     6961 aagaaactgg cgcaatggta tctggaacag ggaaaaatcg agcagatgcc gagctatgac
     7021 cagattcaaa cagtgatgaa acggttgccg gaacatatga aggagcgcgg caggcgcacg
     7081 gggtcggctt ataaggcttt gctgccttac attgatagag attggacggc gttgaaaccg
     7141 aacgatgtct gggtgggcga cggccacagc tttaaagcaa aaatccggca tccgatgggc
     7201 tatctgttta cgccggaagt gacaatgatt gtggacggtt gcagcggtgc ggtcgtgggt
     7261 tggagtgtgg cgctgtcgga aacggcggta gcggtggcgg atgctttgcg ccacgggatg
     7321 acgcatttcc cgccaccttt gggttattac tcggataacg gctcgggtga aacgggcgat
     7381 atgctggata aggaaacgac gggtattttg ccgcgtatcg gtatcgagca ttttacgggc
     7441 attccgggca atccgcaggg gcgcggcaag attgaacggc tgtggcagac cattacgatt
     7501 cctttggcca agcagtatgc gacttatcag ggcaaggatg cggatgccga aacattgaga
     7561 aaggtatcga atgcgctggc gagtgcgcag aaggtgcaga aaaagggcaa ggaactgagc
     7621 aaggcgcagg aagcggcgct gtcggcggca ccgacttggg cgcagtttat agcggatttg
     7681 gaagaagcgg tacgccgata caactttgaa cacgagcacc gcagcctgcc gaaaaatccg
     7741 gaagcgggac ggcattttac gccgatgaaa tattacgaat accggatgga aacggacggc
     7801 atgagggtga aaacggatgt attaagcccg ctggagttgg attttatgta ccgtccggag
     7861 gaagaacgca tccccgaccg cggagcggtg tctctgcaca ataatactta cttccaccaa
     7921 gaattgctgg attacagcgg tcagaaggta cgggtggctt acgacatcca cgatgccgac
     7981 catgtgattg tgaaggatat gcagggcaag gtgatttgca aagcggtatt caacggcaac
     8041 aaacgggcgg catttgcgga aacgcggatg gaacaactgg ccgaacgccg ccgcaaaggc
     8101 caggccaaac gtctgcaaga caagatggat ttaatcgagg cacaacgcca atcggccgcg
     8161 ccgattatcg agcagcagcc tgatttcggc gaatttttga agctggaaac ggaaagcggc
     8221 gggttggtag aggtggaaaa caggccgtct gaaagcggca ggggcaagaa gaagttacgc
     8281 gattttttat gggaagacgc cggttagtaa aacggcacat ctttgacact ttttaaggaa
     8341 aacgaaaaat gattgagaaa ttaagacaat ttctggaaac cagcggcatg agccaaaaca
     8401 aggcggccga ccgcatgggc gtatcgagaa gtgcattgtc gggctatttg aacggcaaat
     8461 acgacggcga tattgcgggg atggataaga aagccgcgct gtttcttgaa caggaaggcg
     8521 accgggcgga attgaaaaag ctggatattc cctatgttga aacgggcacg gccaagaaga
     8581 tgaaaggttg gctgggtttg gcggcatggc tggggcagct cggtattgtg tatggcggtg
     8641 cgggcttggg taaaaccacg gtattgaaac aatatgcggc cactaacccg ctggctttgt
     8701 tgattgagcc ggatacaggc tatacggcaa aggtgttgtt gcaggaaatc tgccatgcac
     8761 tggatttaag cgagagaggc aatatccatg agctgacgga gcggattatc aactgcctga
     8821 agcgtgataa gaagagccgc agcccgcgtg atgcacaccg tattttgctg attgacgagg
     8881 cggaacagtt accgacacgc gcactggaga gcctgcggcg gatacatgat aaatcgggcg
     8941 tggcggtggc gttggtgggt atgccaaagc tgctgctgaa tctgaagggg ccgaacagtg
     9001 aattcaagca gttgttttca agggtatcgg tcaagatgga attgggcgag atgctgccgg
     9061 aaacggattt aaaacagatt gcggcggcag tgttgaagac gaacgatgag gcggtattgc
     9121 agaaggtggt gaaaacggcc aaaggcaatg cgcggaaact gtcgaagctg ctgctgattg
     9181 tggactattt gttgcaggtt aaccctgatg ttgatttgga tgatgatgta atcgaacacg
     9241 cggaaaccta tttaatccac taaacctttg acagataagg caataatttt tttaccttgt
     9301 tattttgtta agttattgaa attaaaggaa aactaaaaat ggaaaatttg aaaatgcggc
     9361 atctgctcgc cataccggcg attgtgattg cggtaacggc gtgtgcgaac cgctgcaccg
     9421 agccggtgcc ggcgtggagc ctgtatcaga acgagaccag tcagcagcgg ctgatgcggg
     9481 aaaaacagaa tgcggctgac tttgcggtat tgcaggcaga aaaagcctat gaacggatga
     9541 gcgataaaga gcggatgaaa ggggtggttt atgaaaactg agacgaaaat ctgcaaaaaa
     9601 tgcggagaag agaggccgtt gttccgtttc aaaaaagacg atgcgtatgc gggcgggtat
     9661 aggcctgtat gttacgagtg tttaaacgcc agagcaaagg aactgaaggc agtccgagac
     9721 agtatggcgg aaagaaagat tatctctaaa ggcatcagca taaatggagg gaaaacgcat
     9781 atggaatttc ctgaagaacc tgaatttatg aaaaacaaat actggaccgt tacaaaaacg
     9841 gattatagaa gaccattaaa aggactttaa ccatgagtaa acggttggca cgacggaaga
     9901 tgccgaaaca cgaattgaaa cgggttaaag cgttcgccct gaaacaggcg gtcgagaaga
     9961 ttcgggcacg gtggggcgag aaagcgatac ggcttgcctc aacagacaac aaacagactg
    10021 attaaacaag gaataagaaa atgatgaatg aaatgaatgc ggtagcggct ttgttggtta
    10081 ttgccatatt gctggcggcg gtaatgactg tgttggtcga gatattggtc gagcaacggg
    10141 ttaaggaacg gctggggaaa ctgctggata aggcgtgccg accggatgac gggtggcagg
    10201 aatgataagg aaggaaatgt gatgaatatg gtactgacaa tttatttttg gttggtgggg
    10261 ctggtctgtt ttgcccgttc cgccaaagag acttattacg cggaatcttt atacggtctg
    10321 ctgactgggc tgttgggggt gatgtgttcg gcttttgtgg tgtcagtgtc ggtggcaagg
    10381 gtgatggggt ggttgaaatg aatcaattga aaatgacgat cgccaaacct gagacggaag
    10441 attttgaaga tgcctgggca tttatcagaa tgcttaattt ggtgacttat gatttgaacc
    10501 ctctgaaaac tgataccgac ggcgaatacg aatatttggc ggatgaagac aaatctgatg
    10561 tattagatgc cgttgtcgaa aaatttaacg aatgtagtct tgagtggatg ttgtcggcgc
    10621 tgcaagcact gatgtcgccg gagatgggga ttatcaacca agattcggat acgttggagt
    10681 tgcatcccaa actgaaaggc ggtacggaat gaaagtgcgc tgccctacct gcggtgcggt
    10741 gatgagctta gatgtattaa tcgcccatga tgatgcagcg aagctctgat tgccctgaca
    10801 ggcatttcag acgacctttt taaggcggta ttgcggtatc tgacgctgtt tcgccccgcc
    10861 gaaaaggatt taagttttaa ccgagtttca aagctcgtcg gcgagattgc gccgatgata
    10921 cgggacggaa agattgtccg caaccgcaaa acttatccgg ccccgcgcga ggcttggatt
    10981 tgggcggcaa cgcgatgcct tgaggcacgg gacgcgggaa aactgacacc gccactgacc
    11041 agccacggtt atttgttgga aaacattacg ttttggtcgc ctgaaaagac ggcgggaacg
    11101 gcggttttgc cctctcccca accatctcac acgagagagg tggcaagcac caaattgagg
    11161 agcgggttgg gcggcttgat ggagtggtca aatggaggaa aacaatagct ggctgaaaaa
    11221 agcaatcgcg cagggtttca tgatgctcgc cgccctaaac ctcaaaggcc gccctgcctc
    11281 ggcggatttg acggcagtcg ccgaactttg gttggtcata ctaagcggcc ggtcgtggca
    11341 gccggagcat gacgggatca ggatacaggc agcctttagg gctatcgcgg cgtcctcgtc
    11401 agagtggcca aaccctgccg accttatcaa gcacctgcca ccgggcgaag tcaggatggt
    11461 gccgaggctg gaaaagaagc actgcccgac ggaatacggc aaagagcagg ccgccgaact
    11521 gaaaaagatt gtcggcagat tgaaaaacgc accctgcatg aatagagatt ggatacacgg
    11581 gcaacgccac cggtcggtgg acgaatgtaa aaggatttat gccgaaaggc agaagggaaa
    11641 taaatagcca aatgagtacg ttttttgaca atcaggaatc cgacttaata aacgaatttc
    11701 tgacggaata ctgggaagag tttgaaaaat tctccgccaa caacggattt gatgacgatt
    11761 tattgaagca aatatcaatg gaattggaag gacataggaa ccattatgag taacttagac
    11821 atgaaacaat acaaacagga tgccaaaggc aatctcgtgc cgctggccaa tattaaagaa
    11881 atcgacctgc tgcgcgacga gctggtgcag gaaattgccg ccaaagcccg cgcggtacag
    11941 gataacttga tggcgttcaa acgcgaggcg atggacgata tcgcagcgtt tgtacagttg
    12001 agtgccgacc gctatgacgt atctgtcggc ggtaagaaag gcaatatcag cctgcacagc
    12061 tttgatggag cgtaccgcgt caaccttgcc atacaggaca cgttggtatt tgacgaaggt
    12121 ttgattgccg ccaaagccct gattgacgag tgtatcaacg aatggacgga aggcagtcgc
    12181 acggaattga aaacactgat taacgcggcc ttccaagtgg acaaagaagg caatatcagc
    12241 actgcccgcg tcctcggtct gcgccgcctg caaatcacgg atgaaaaatg gcaacgggcg
    12301 atggatgcgc tctccgacag tttgcaggtg catatcagca aaccgtttgt gcgggtatat
    12361 cagcgcggcg aggatgggga gtatcagtta atgaatttgg atgtggcgaa ggtgtaggaa
    12421 aaatgaacgc aaatataatg atttatttga ttgaagttaa tgatgggccg gttgcaaaag
    12481 cactgcgaag ttttcagcag gacaaggccg caatgcggca ggcgtggctt gattgggcgc
    12541 gagaacactt gccgtcagac gcgctaatgc gggaatggag cgatgggcaa gtggcagggt
    12601 ttgcgttccc ttcaggcata cccgatggat ggaaaaagcc caataagaac ggaatttgtt
    12661 ggccacggca aaacaaccca atcctcaaaa ccatgccact taacaaaaga tttaaacgcc
    12721 ccgaagagta tttggaagaa gttggcatca ccgcaccaac aatgattttt gaaaagaata
    12781 gcgacggaga gacttgggca tcttggggta tcggtaattt ctttaatcct gttcagttcg
    12841 tttgggcggg attggaggag gacgcgccaa agggcgtggt aactcctgat tatgcctacg
    12901 agcttagaga aggggctaag agaatcagaa acggctgtac gatgcagccg cccgaagatt
    12961 ttgattggca gaatctgtta ccgggctgcc gtgtcattcc tcggtatgag tgggattact
    13021 tggttgggaa atggcaggaa acacggaatg atgccgaaga acaggaggct taaatggcaa
    13081 aaatcattat tgaaatcgaa gacctgcccg agggcaccga cattaatttt aagggcgacc
    13141 ttccggcggc ggatgcaaaa gacaaaacag gtgcgcagca aacggctgtg ttaatcagca
    13201 agatgattca ggcggcgcag atgatggcat ccccatccgt aaacattagg ccgtctgaga
    13261 ctgcaaatcc ataacccgcg cggcacggtc tgccgcattt aaatctaaat aggagtcaaa
    13321 aagtgaataa atccgaatta atccaagcca tcgccgacga ggcggaattg agcaaacgcg
    13381 ctgcggcgga gtttgtcaat gcgtttgtca gcgtggtaac gcaagaactg aaagacggaa
    13441 acgacgttac gctggtcggc ttcggcacgt tccacgccgc ccaatccgcc gagcgtcagg
    13501 gacgcaatcc gaaaacgggc gaaccgctga ccatcgcggc gcgcaaaaca cccaagttcc
    13561 gctcgggcaa ggcgttaaaa gatgcggtaa acggttaaac gtctgaaacc ccaaatccaa
    13621 gcggattttt gaaaagtccg cttcagtttg aagttttagc gacgaggaaa accatgatga
    13681 acacaaacga acagtacaaa cgcaaggggc tgattgcgaa aatcaaaatc gcgcaaagcc
    13741 agcttgggat ggaagacgat gtttaccgtg cgatgctggc gcgtgtgtgc ggcaaaacat
    13801 cctgcacgca gatgaatcta gccgaattgc aggcggtggc ggcggaaatg aagcggatgg
    13861 gctttaagca gaccgcaccg aagggcaaag gtatccgccc gcatttgaca caggaccgtg
    13921 ccgcgctgtt gaacaagctg gaagcgctgt tgacggtggg cgataaaagc tggcagtacg
    13981 ccgacgggat ggccaaaagg atgttcggca aggatttggt acgatttttg acacccgagc
    14041 agctatacaa actggtgcag gcgctgcaaa tccatatcaa caaagcgaaa aaggcggcag
    14101 agcaatgatc agggtggatg aagaagattt tgaggcggtg cgccacctgc tgcccgaaag
    14161 tgtgctggca ctgattacga tcatcgggct gacagaaacg gtggagctgg tgaaaaactt
    14221 aggcggcacg acctacccgt tgcggcaggg acgcaccaaa ggcagcgaat cgcggctggc
    14281 ttatttggag gaaatcgtcg gcggtgcggc gatggaaaaa atggtggaag cactggcccc
    14341 ctgcgatttg tttattccga aatgcgagca ggctttgctg gaattgcgtg accgccatat
    14401 ccgccgccgc ttcgatgcgc agacgggtaa aggcgtaccg gcttatgagg cggtgaacga
    14461 tttggctttg gcgcacgcgc tgaccgaccg ccatatctgg cggattctga agaagccgga
    14521 taatgagtgt gagcaaggcg ggttgtttta aattataatg ccgtggggat aacacggcat
    14581 tttttatggg aaatcaagat gaagacgttt tatgttctga tgttggccgc aatgcttgcg
    14641 gcttgcggcg gacaagaaaa caagcctgct gccggcgaaa gtcaaaccga taatcgggcg
    14701 cagcaatatc agaatcagca aaaacagatt gccgcacgtt tggctgatga agaatatttc
    14761 atcggtgaaa acaatttgga acaaatccgt ctgcacgcca atttgaaaga gcgcgctcaa
    14821 aagctgttgt ctttattggc gcaggccgac aaggaaagcc gtgtgtgggt tttgcccggc
    14881 gatgcagcca aaatcaaaga attcaatgcg gctttcgcgg cggtggcaaa atctgccgag
    14941 gaatctttcg gcggcccttt tcttgcggat aaggcggggc tttatcaatg taccgctgcg
    15001 gccaatgcgg cttacgatta ttttttcgcc cggcagagaa aggatgcgct gaccgaaaat
    15061 taccgtcaac aatacacgga caacatagct gcctgccaag ggcaaatccg caccccgcca
    15121 gccgcccaag cgacagtgta tgcgcgtaaa ggtatcaggc tgccgttgaa taattgtttg
    15181 ccggtattgg ccggagatga agaatttgat acctacacct gcccgatgga agtaaaatag
    15241 gccgtctgaa acccagcccg agagcaaaag ccctcgggct ttttgctgcc tgtctgacat
    15301 ggggcaggcg cggtgccaaa taagcggaca ggataatcaa ggctcgataa cagactaagc
    15361 aggacgaatc tatgagcaag attattgtgc tgaccgcagg ccacagcaac accgacccgg
    15421 gtgcggtcaa cggaagcgac cgtgaggcgg acttggcgca ggatatgcgc aatatcgtgg
    15481 ctgctatttt gcgcgatgac tacggtttga ctgttaaaac cgacggcacg ggcaaaggca
    15541 atatgccgct gcgcgaagcg gtcaagctga ttcgcggctc ggatgtggcg attgagtttc
    15601 acaccaacgc tgccgtcagc aaagcggcga caggcatcga agccttgagt accgt
//
LOCUS       DAWFLT010000042        15573 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWFLT010000042 DAWFLT010000000
VERSION     DAWFLT010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15573
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.042"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(181..2109)
                     /gene="dnaK"
                     /locus_tag="WGR74_001297"
     CDS             complement(181..2109)
                     /gene="dnaK"
                     /locus_tag="WGR74_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ0905712.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDHIIAEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKAEALGTASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2317..2589)
                     /locus_tag="WGR74_001298"
     CDS             complement(2317..2589)
                     /locus_tag="WGR74_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905713.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEKFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2775..3461
                     /locus_tag="WGR74_001299"
     CDS             2775..3461
                     /locus_tag="WGR74_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0905714.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3593..3931
                     /gene="erpA"
                     /locus_tag="WGR74_001300"
     CDS             3593..3931
                     /gene="erpA"
                     /locus_tag="WGR74_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ0905715.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            3948..4094
                     /locus_tag="WGR74_001301"
     CDS             3948..4094
                     /locus_tag="WGR74_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905716.1"
                     /translation="MPSERSDGIFTFRKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            4091..4555
                     /locus_tag="WGR74_001302"
     CDS             4091..4555
                     /locus_tag="WGR74_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ0905717.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLKENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     ADGEMPEYLELHGGREDDISAPSQPELPTRNIKPLLFGLLIAFLGYLLGKIF"
     gene            4590..6101
                     /gene="ubiB"
                     /locus_tag="WGR74_001303"
     CDS             4590..6101
                     /gene="ubiB"
                     /locus_tag="WGR74_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ0905718.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGRRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFHNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
     gene            complement(6458..7276)
                     /gene="cysE"
                     /locus_tag="WGR74_001304"
     CDS             complement(6458..7276)
                     /gene="cysE"
                     /locus_tag="WGR74_001304"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ0905719.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
     gene            7460..8038
                     /gene="grpE"
                     /locus_tag="WGR74_001305"
     CDS             7460..8038
                     /gene="grpE"
                     /locus_tag="WGR74_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ0905720.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            complement(8062..8202)
                     /locus_tag="WGR74_001306"
     CDS             complement(8062..8202)
                     /locus_tag="WGR74_001306"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905721.1"
                     /translation="MKKNRQAAYGLTVNAETKCRLKPDRRVQTALPLYFDRFIRKTRF
                     RK"
     gene            complement(8162..8377)
                     /gene="nqrM"
                     /locus_tag="WGR74_001307"
     CDS             complement(8162..8377)
                     /gene="nqrM"
                     /locus_tag="WGR74_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ0905722.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(8403..9458)
                     /locus_tag="WGR74_001308"
     CDS             complement(8403..9458)
                     /locus_tag="WGR74_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ0905723.1"
                     /translation="MPSETRLPNFIRTLIFALSFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVHLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            complement(9611..10828)
                     /gene="nqrF"
                     /locus_tag="WGR74_001309"
     CDS             complement(9611..10828)
                     /gene="nqrF"
                     /locus_tag="WGR74_001309"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ0905724.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPEEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            complement(10842..11435)
                     /gene="nqrE"
                     /locus_tag="WGR74_001310"
     CDS             complement(10842..11435)
                     /gene="nqrE"
                     /locus_tag="WGR74_001310"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ0905725.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPANQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYGFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            complement(11439..12065)
                     /locus_tag="WGR74_001311"
     CDS             complement(11439..12065)
                     /locus_tag="WGR74_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ0905726.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            complement(12065..12841)
                     /locus_tag="WGR74_001312"
     CDS             complement(12065..12841)
                     /locus_tag="WGR74_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ0905727.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVKDAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSVGAQ"
     gene            complement(12834..14066)
                     /locus_tag="WGR74_001313"
     CDS             complement(12834..14066)
                     /locus_tag="WGR74_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ0905728.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYTLAD
                     ALGINMSSEAGVSGKMLFGAIYFLPIYATVFVVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGITFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            complement(14069..15412)
                     /locus_tag="WGR74_001314"
     CDS             complement(14069..15412)
                     /locus_tag="WGR74_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ0905729.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPVITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFKRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTGRVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFNTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
BASE COUNT         3558 a         3705 c         4273 g         4037 t
ORIGIN      
        1 gtactggttt aaatttaatc cactatataa aaaatttcca gagaaccgat acaacagttg
       61 gaacttgggt ttgggaatat tacggtagat gaacttggaa cctctgttat gctatggtct
      121 tttatctcaa ttgaaaaaag cgcgaatcaa acggttcgcg cttttttcag acggcatcaa
      181 ttattttttg tcgtctttta cttcttcaaa gtcggcatct acgacatcat cgtctttctt
      241 tgcagaagca ttggcttgtt cgctttcgcc tgcttgggct tcagcttgtg cttgagcgta
      301 aaccatttcg cccagttttt ggctggctgt gcccagcgcc tcggctttgg catcgatggc
      361 ggctttgtcg tcgcctttca cggcttcttc ggcttctttc agcgcggctt cgattttttc
      421 tttttcggca gcgtcgagtt tgtcgccgta gtccgccaaa gattttttca cggagtgaat
      481 cagggcttcg gcttggttgc gggaagcgac caattcagtc agctttttat cttcctcagc
      541 attggcttcg gcatctttca ccatgcgttc gatttcttct tcgctcaaac ctgaagaacc
      601 ttggatggta atgttggctg ctttaccggt gcctttgtct ttggcggaaa cgtgcaggat
      661 gccgttggcg tcgatgtcga aggttacttc gatttgcggc ataccgcgcg gtgcaggtgc
      721 gatgtcgccc aagttgaact gacccaaaga tttgttggca gaagcgcgtt cgcgttcgcc
      781 ttgcagtacg tggatggtta ctgcgctttg gttgtcttcg gcggtagaga acacttgcga
      841 cgctttagtc gggatagttg tgttcttttg aatcagcttg gtcatcacgc cgcccatggt
      901 ttcgataccc aaggacagag gggttacgtc cagcagcaat acgtcgctgc ggccgccgct
      961 taacacttcg ccttgaatcg ctgcgcctac ggcgacagct tcgtcagggt tcacgtcttt
     1021 gcgcggctct ttgccgaaga agtctttaac ggcttcttgt actttcggca tacggctttg
     1081 accaccgacc aaaatcacgt cgtcgatgtc gccagtgctc agaccggcat ctttcaatgc
     1141 ggtgcggcaa ggctcgatag agcgggcaat caggtcttca accaggcttt cgaatttggc
     1201 gcgggtaatt ttcatcgcca agtgtttcgg gccggttgcg tccatggtga tgtacggcag
     1261 gttaatttcg gtttgctggc cgctggacaa ttcgattttg gctttttcgg cagcttcttt
     1321 caggcgttgt agagccatca cgtcttgttt caaatcaatg ccttgttctt ttttgaactc
     1381 ggcgatgatg tggtcgatga ggcgttggtc gaagtcttca ccgcccaaga aggtatcgcc
     1441 gttggttgcc aatacttcga attgtttgtc gccgtcgagg ttggcgattt cgatgatgga
     1501 aatatcaaaa gtaccgccgc ccaagtcata tacggctact ttgcggtctt tgttgtcgcc
     1561 tttgtccata ccgaatgcca aagcagctgc agtcggctcg ttgatgatgc gtttcacgtc
     1621 caaaccggcg atacggcctg cgtctttggt agcttgacgt tggctgtcgt tgaagtaggc
     1681 agggacggta atcacggctt cggttacttt ttcgcccaag taagcttcgg cggcttcttt
     1741 cattttacgc aggacttctg cggaaatttg aggaggagac agctctttgc cttgtgcttt
     1801 tacccatgcg tcgccgttgt tggctttgat gatttcgaaa ggcatagatt cgatgtcgcg
     1861 ttggacttct ttgtcttcaa atttgtggcc gatcaggcgt ttggcggcgt aaatggtgtt
     1921 tttagcgttg gttacggctt ggcgtttggc aggcgcaccg acgaggattt cgccgccgtc
     1981 caaataagcg ataacggacg gcgtggtgcg tgcgccttct gcgttttcga tcactttggt
     2041 ttgaccgttt tcggaaatgg ccaaacaaga gttggttgta cctaagtcga taccgattac
     2101 ttttgccatg tggataatcc tatttgattt tgcttatttt gagaaatatg ttggaacatt
     2161 ttgtcccgat gggctgtaaa tagggcgggc ggcgggctgt ttcaagctac agcatggcta
     2221 taagtatata actttatgaa tatattggtt ttatatttga tttaatacat ttggctccaa
     2281 tgcatttaag cataatattt caaatggcag gcaggtttat tcatagacga tgccggcgag
     2341 catttcctgt tcgttcaagt tgccgtactc tttttcccag tcgtgtgaag actcgatgat
     2401 gtcgcattct ttggaaaggg agacttgttc tgcatccata tctttggcgt tcagtatgtt
     2461 gaatttttcg cacagggatg cggataaagt gatgtcaggc tgtttggctt cagaacggtt
     2521 ttcttggaag gcaaagcaga atgcggtaaa tgccgcagca tagataagat atttgccagt
     2581 tttcttcatt ttctatcctt tttctgtcaa ctcaggatta aacctatgga aaatcttaaa
     2641 aattatgtat tgagtaagaa aaatcataat ttaaatttag tttatcatag ttattccgtt
     2701 tttgggatag ctaaggtaaa atatatttca tgtttacttt agatgattga atgaagggga
     2761 gcggaaggat atttatggaa acctttaaag acagactggt ttttttatgg aaaagcgaag
     2821 cgaagcaggc aaaaatcgca tccgatattg aaatgacgat tgcgggcttc agcaggatat
     2881 ggaatgaagg cggtctgccg aagtctgaaa cattaaaaaa aatcaagcag ttgaaggggt
     2941 gtagtatcga ttggctgctg accggggagg gtaatccgtt tccggatgaa gccccaaaaa
     3001 aatcccttgc ttacgatacc ttgggaaatg aagtcgatac ggacgagttt gtctttgtgc
     3061 cgagatatga tattcgggcg gctgcgggat acgggcagtt tgtcgatcat gaggaaccgg
     3121 tatttacaat ggcgttcaga cggcattgga ttgagaatta tgttacccgc gatacgaaaa
     3181 acctgtctgt aatttccgtc aagggggatt cgatggaggg ggttttgaat gacggcgatt
     3241 cgattttggt caatcatggt gaaaatacgc cgagggacgg tctgtatgtg ttgcggatta
     3301 atgaaaatct gctggttaaa cgtttacaga ttgtaccggg cgggattatc aatgtgattt
     3361 ctgcaaacga ggcttatcct gcttttgaaa tcaatttgaa cgatttgacc gatgatgtgg
     3421 agattatcgg gcgtgtcgag tggttcggca ggacgatttg agtttggggc ttgaaattgc
     3481 aggcggtcaa acttatctat tggaacaatt cctttttcaa aggcgaagcc tgcttgcctt
     3541 tgaagggggt ttgagagaga atgcagaaaa tattatatta aggaataaca ccatgtcgga
     3601 tgaaagccct attattttta ctgacagctg ctgtgccaaa gttgccgatt tgattgccga
     3661 agaaaacaat cccgatttga aattgcgggt ttttgtcaat ggcggcggct gttcgggttt
     3721 ccagtacgga tttacttttg acgaaatcaa aaacgacgac gattttgaaa ttgagaaaaa
     3781 cggtttggtc tttttggtcg atccgatgag ctatcaatat ctggtcggtg cggaaatcga
     3841 ctatacggaa agtttgcagg gttcgcaatt cgtcatccgc aatccgaatg cggaaacaac
     3901 ctgcggttgc ggatcgtcgt tttccgtatg accgcttggt ttgtgtgatg ccgtctgaac
     3961 gttcagacgg catttttact tttagaaaat atattatcgg gatgaattca catataatcc
     4021 gattgtttga agatgaatcg ggtttcccga aaggaacggg cggaacggta tcaggcgtat
     4081 ttgttccctt atgattgaga tgagtaaaga ttaccgaaac gatttgtacg atgtatatgt
     4141 ttcttacccg ccccaagtgg atcgcgggct tatccgggag tgccttaagg agaatctcgg
     4201 cgaggaaaag gcggaaggat tgatcgaatc gctcgattcc aaacctcaag tgctggttga
     4261 ggaaaaatgc acttgggcga aacgggaaga gttgcatgat tatttcagct atttgggttt
     4321 ggatattatt acccggagat atatggagtt agaaacggtc gtgccgccgg aggaaggtga
     4381 gggcgaagga gagggcgcgg atggggaaat gcccgaatat cttgaacttc atggcgggcg
     4441 ggaagatgat atttccgcac cttcgcaacc cgaactgccg acccgcaata tcaaaccgct
     4501 gcttttcggg ctgctgattg cctttttggg ctatctgctc ggtaagattt tttgattgtc
     4561 cgataaatgc tgtattcggg attttatata tgaaatggtt gaaacgcctg acggtcattg
     4621 tcgggacttt ttaccgctat cggctggcag gtctgtgtgc ttcgctgatg ggtagcggtt
     4681 ggatatgcgc tctgctgaaa atgatgccgc agtcgtccaa attgaaaaac gaaccgcctg
     4741 ccgtccgtct gcgccttgcc ttggaaagcc tggggccgat tttcatcaag ttcgggcagg
     4801 ttttgtccac acgccccgat ttgattccgc acgattacgc cgtcgaactg gcaaagctgc
     4861 aagacaaagt gccgcctttt gacgcgcggc tttcgcgtga acaaatcgaa aaatcgttgg
     4921 gtcagtccat cgaaaagctg tatgcggaat ttgaaaccga gcccatcgcc agcgcgtcca
     4981 tcgcccaagt acacaaagcc cgcctgcatt cgggcgaacg agtggcggtt aaagttttgc
     5041 gccccaacct tttgcccctt atcgaacagg atttgtcgct gatgcgtttc ggcgcggcct
     5101 gggtcgaacg cttgttttca gacggcaggc gtctgaagcc gctcgaagtg gtggcggagt
     5161 tcgacaaata cctgcacgac gagttggatt tgatgcgcga agccgccaat gccggacagc
     5221 tcgggcgcaa tttccacaac agcaatatgc tgattgtgcc caaggtgttt tacgactact
     5281 gcaccagcga cgtgctgacg attgaatgga tggacggtac gccggttgcc gacatcgcca
     5341 aactcaaagc agacggcatc gatttgcaca aactcgccga ttacggcgtg gaaatctttt
     5401 tcacgcaggt cttccgcgac ggctttttcc acgcggatat gcaccccggc aatattttgg
     5461 ttgccgccga caaccgctac atcgccctcg atttcggcat cgtcggcacg ctgaccgatt
     5521 acgacaaacg ttatctcgcc atcaatttcc tcgccttttt caaccgcgat taccggcgcg
     5581 tcgccaccgc ccacatcgaa tcgggctggg tgcctgccga cacgcgtgcg gaagaattgg
     5641 aagccgccgt ccgcgccgtg tgcgaaccgg tgttcaacaa accgatttcg cagatttcct
     5701 tcggcttggt gctgatgcgc ctgtttgaag tcagccgccg cttcaatgtc gaaatccaac
     5761 cgcagcttgt gctgctgcaa aaaacgctgc tcaacatcga aggcttgggc cgccagctcg
     5821 atcccgattt ggacttgtgg aaaaccgcca aaccgttttt ggtgaaatgg atgaacgggc
     5881 aggtcggccc taaagccctt tggcgcaacc tcaaaaacga agcccccgac tgggcgcaaa
     5941 tcattccttc attgccgcgc aaaatcagtg cgttgattga tgaaaaccgc cagcaggaaa
     6001 tgcgtgatgc ctatatccat ttggtcaaag tgcagcagcg gcaaagcctg tggctggctg
     6061 tgattgcggt tgttttgctg ctgattttgc ttttgaaata ggctttgtcc gaatcatcgc
     6121 ccgactccgc ccgtttataa ggaaatcggt tatagtggat taacaaaaac cagtacggcg
     6181 ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc aagtgaatcg
     6241 attccgtact atccgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca
     6301 ctatatttcc ggttgcgtgg gaatcgggtg tatcgaataa aaggcatttt gtccgactgg
     6361 caagtgccga catcggcggc atatcaaggc gcaggcttga agcgggcaat gtcgtctgaa
     6421 gcccgtttgg cgtttcagac ggcattggtg cggatattca aatcataaag tcgatttcgg
     6481 taaactggat attttgatcc atatccgccg acggtgtttt gagcgatcgc gccacgggtt
     6541 tggcgggtac gccgacaacc gtgatggacg gcggcacgtc tgaaaccacg acgctgcccg
     6601 ccccgatttt ggcattgctg ccgatgcgga tattgcccaa tatcgaggcg tttgcgccga
     6661 tcatcacgcc gtcgccgatt ttagggtggc ggtcgccgcc ttctttgccc gaaccgccga
     6721 gcgttacgcc gtgcaaaatc gaaatattgt tgcccaacac ggcggtttcg ccggcaacaa
     6781 agccggtggc gtggtcgagc atcagcccgt atccgaaacg ggcggcggga tggatgtcca
     6841 cgccgaatac ttcggacata cggttttgca ggaaatacgc cagcgttttg cgcccgtcga
     6901 gatacagccg atggttgatg cggtgtgcct gaatcgcgtg gaagcctttg aaatataaaa
     6961 gcggcagcga atattcgtcg caggcgggat cgcgttcgta gatggctttt aagtctgctt
     7021 cgacgcattt gccgatttgg gtgtcgctgc ccaacgcctg ctggtagatt tcaaacagcg
     7081 cgcgcacgtc cataatcggg ctgccgagtt tgctggaaag gtggtaggca aggacggagc
     7141 cgagggactc gtggcgcaac acggtttggt gcaaaaaact tgccagcatc ggttcggcgg
     7201 agaccgcggc cgcggtttct tcgcggatgg tgtgccagag gtcgaaaccg gttgtgttta
     7261 aatggtcttt tttcatgagt gatgacgttt gaaaatcgat atggtcggca gtatcttacc
     7321 gtctatatta ttttttcggt aggggatttg aaaatgaatt tgaaattctc tgcctttgct
     7381 tgaagtttct tgaaaatgtc cttatcttgc gcgggtaata actggatttt gatttccaat
     7441 ttgttttaag ggatacgata tgagcgaaca gacacagcag caaaacagtg aagaagcggt
     7501 tgaaaatgtg gaggcggtgg aaaccgtcga gacagtagga aatgcggacg gtgtgcagga
     7561 acaggctgcc gcagagccgg cttatgagga tttgcaggcg cggattgccg agctggaagc
     7621 gcagttgaaa gacgagcagc tgcgcgcttt ggcaaacgag caaaacctgc gccgccgcca
     7681 ccagcaggaa attgcggata cgcacaagtt cgccggacag aagtttgccg tggaaatgct
     7741 gccggtcaag gattatctgg aaatggcgct tttggatcag agcggcaatt tcgatgcgct
     7801 gaaaatgggc gtgcagatga ctttgaacga gttgcagaaa gcatttgatg ctacgcaaat
     7861 caaggaaatc aatcctaaag cgggcgataa gctcgatccg aatatccatc aggcgatgca
     7921 ggcggtggca agcgaacagg aacccaatac cgtggtgggc gtgatgaaga agggttatac
     7981 gctgtccgac cgcgtgttgc gcccggctat ggttacggtg gcacagaagg aagcctgaag
     8041 gcgtctgggg aataatctga tttatttcct gaagcgcgtt ttgcgtataa accgatcgaa
     8101 gtaaagcggc aatgccgtct gaacccgcct gtcgggcttc agacggcatt ttgtttcggc
     8161 gttaacggtc aacccgtatg ccgcctgcct gtttttcttc atccagtttc ttttgcaggg
     8221 tgtcgcaagg tgtgtcgcag tcgcacattt ttttcatacc caaggcagta atgccgccgc
     8281 aactgccttt gatgctgcgt ttggagaaaa tatagccgac cgccataccg atgatgacgg
     8341 tcaggaagat gccgaaggta aggagcaggg ttttcatggt gtttcctaat cggtttgtat
     8401 gtttagcgga gcagtttttc aaattcggaa gacatggcgg tgcggtagcc gcctttatcc
     8461 ctgacaatca ggaaaacagc gagtttttcg cgctctgcca gctttaaggc ttcggtttcg
     8521 cccaatacga ataatcctgt ggacaagccg tccgccgtca tcgcactgtc tgcgaccacg
     8581 ctgatggagg cgaggttgtg gctgatgggt cgtttgttgt tcggattaat gatatgggag
     8641 aggcgtttgc cgcttttatc gacgtggaaa atacggtaat cgccggaagt ggcaagcgaa
     8701 cggttgttca gcgggacgat aatctgcgta ttgccgcctt ggacgatgtt gggctgttcg
     8761 atgccgatgc gccaaggttc gccgcgcgcg tttttgcctt tgccgtgcaa ctcgccgccg
     8821 atttcgacca gataattttg aatgccgtat ttttccagtt cgcccgcaac tttatcaacg
     8881 ccgaagcctt tggcaatcga agataaatcc aaataggcct tggggtgggt tttgctcaag
     8941 gaagcgtaat ctttgccttg tttcaaaatg attttgtcta tgcccgtata agatgctgct
     9001 tgtttgattt gttccggcga cggttcacgg gtaacggatt tgtcggggcc gaatccccaa
     9061 aggttgacca aggggccgac ggttacgtcc agcgcgccgt gtgtcaggcg gttcaggtgg
     9121 acggcttcgg cagtaacgtg tgcgaagtcg cttgaaatgc ggaggggctt gccggctgtg
     9181 tgttggttga accggctgat ttcggagtcg ggctgatagg tggacatctg ccggttgact
     9241 tctttaagcg cgtcatcgat gcgcttttgt atttcggcag gtgaggggag tttgtcccga
     9301 ttatttgaaa ggtatttgac ggtataggtc gtgcccatcg tttcaccttg cagggtaacg
     9361 gtttgcgcgg tttgttccga acaggcgttc aggaagataa aactcagggc aaatatcaag
     9421 gtgcggataa agttcggcag gcgtgtttca gacggcatag tgtttgacgg ttttggcaaa
     9481 tggtttgaat tatatcgcaa aacggccggt atgtttctat gccgatgccg tctgaagggt
     9541 gttcggatgg catcggcata gaaaaaggaa gaaaccgagg tttcttcctt ttgtatttga
     9601 agccgaatac tcaaccgccg aaatcatcca agaggatgtt ttcgtcttcc acgcccaagt
     9661 ctttgagcat tttgatcacg gactggttca tgattggcgg accgcacata taaaattcgc
     9721 agtcttccgg tgcttcgtgg tttttcagat ggttttcgta aaccacgttg tggatgaagc
     9781 ccgtgtagcc gtcccagttg tcttccggca gcgggtcgga cagggcgacg tgccacgtga
     9841 agttcgggaa ttctgctgcg agttggtcga agtcttcgac atagaacatc tcgcgtttgg
     9901 aacgtgcgcc gtaccaaaag gtaatcttac gtttggagtt caaacgtttc aactggtcga
     9961 aaatgtggga acgcatcgga gccatacccg caccgccgcc gataaatacc atttcggcat
    10021 cggtgtcttt ggcgaaaaat tcgccgaacg ggccggaaat cgtaactttg tcgccgggtt
    10081 tgagcgacca gatgtaggac gacatttgtc ccggaggcgc atcaggtacg cgcgggggcg
    10141 gcgtggcgat acgcacgttc agcataatga tgcctttttc ttcaggatac gaagccatag
    10201 agtaggcacg caaaatcggc tcgtccactt tggaaacgta ttgccacaaa ttgtatttgt
    10261 cccagtcttc gtggtattcc tcgggaatgt cgaagtcttt gtaggcaacg gtgtgcggcg
    10321 gggcttcaat ttgaatgtag ccgccggcgc ggaaggggac ttcttcgcct tcgggaatgg
    10381 caagcttgag ttctttaatg aacgtggctt tgttatcgtt ggagatgacg gtgcattccc
    10441 attttttcac gccgaacact tcttcgggga cttcgatgtc catgtcggtt ttgacgttga
    10501 cttggcacga cagacggcag ccttcgcgtg cttcgcgttt gctgatgtgg gacagctcgg
    10561 tcggcaggat gtcgccgccg ccgcttttta cgacgacgcg gcattgtccg cacgaaccgc
    10621 ccccgccgca ggcggagggg ataaagatgc cttcgttggc aagcgcgccc aagagtttgc
    10681 cgccggcggg catcgtcagc tctttttcgc cgttgacttt gatggtgatg tcgccttcgc
    10741 tgaccagttt ggatttggca aacagaatca tcagtgccaa aaccaaaacg atgacggtaa
    10801 acatcacgat acctaaaata atctccatac cgatcccttt cttataactg gatgccagag
    10861 aacgacataa acgccatcgc catcaggccg gcggcgataa aggtaatgcc caagcctttg
    10921 aggcctttgg gagcgtccga atatttcatt ttttcggtaa tgcccgccaa agcgacaatc
    10981 gccaacatcc agcccaagcc cgcaccgaag ccgtacacga cggattcgcc gaagccgtat
    11041 tcgcgctgcg ccataaacga tacggcaccg aaaatcgcgc agtttacggt aatcagcggc
    11101 aggtagatgc ccagcgcgtt atagagggcg gggacgaatt tgtccaagaa catttccaaa
    11161 atctgcacca aagcggcaat cacgccgatg aaggtaatga atttcaaaaa ggtcaaatcc
    11221 acgccttcga caatcgcgcc gtctttgagc agcgagtaaa cgagttggtt ggcagggacg
    11281 gacagcccga gtacgaaaat gaccgccaca cccaaaccga atgcggtgga tacttttttg
    11341 gataccgcca aaaacgtgca catacccaaa aagaaggata gcgccatatt ttcaatgaag
    11401 acggatttga tgaagaggct caaatagtgt tccatagctt attcctccgc ctgttcgggt
    11461 ttccaggtac gcagtcccca aatcaaaaag ccgatgatga agaacgcgct gggggcgagc
    11521 aggaacaagc cgttggtctg ataccagccg ccgtcctgca cggtttggaa aacggtgtag
    11581 cccaagagtt tgcccgagcc aatcagttcg cggacggtgg cgacgacaag cagcatcatc
    11641 ccgtagcccg cgccgttgcc gatgccgtcg atcaggcttt ccagcggcgg ctctttcatc
    11701 gcaaatgctt cggcgcggcc catcacgata cagttggtaa taatcagacc gacgaatacg
    11761 gaaagctgtt tggacaattc gtaggcaaag gcctgtaaga gttggtcgac cagcgtaacc
    11821 agcgacgcga taatcgccat ttgcacgata atacggatgc tgttggggat gtagttgcgt
    11881 accagcgaaa tgaagaagct ggaaaaaccg gttaccaaag ctacggaaat acccatcacg
    11941 atggccgtct gaagtttggt ggtaaccgcc aaagccgaac aaatacccaa aacctgcaag
    12001 gcaatcgggt tgttgtcgat aaagggtgaa aacatcaagt gtttcaagcg tttcatatca
    12061 gccattattg tgctcctact gatttcaatt tgttcaggta ggggatatag ccgttttcgc
    12121 caaaccagta ggcgaacgaa ccttgcacgc ctttggaagt cagcgacgcg ccggagaggg
    12181 catctacgcc gtgttctttg tccgaacccg cgcctttgcc gacgtgcagg gcgagtttgc
    12241 cttgtccgtc aaacagtttt ttgccgacga atttttgctg ccacaacgga ttgccgattt
    12301 cgccgcccaa gcccggggtt tcgccttgtt cgtagtaggt aatgccgttg atggtgttgc
    12361 cgtccggttg aatggcgaca aagccgtaca tgaccgacca caaaccgtta ccgtgcatcg
    12421 gcaggatgat ttggccgatt ttgccgtctt cgccttttac caaataaacc tcagtgtatt
    12481 tggcacggct tttgatgcct gccaaatcgt cttcgggttt gattcggatg ctttgcgcgg
    12541 ggtctttgcc tgcgatgcgc gcgctgaagt cttttggcgc gtctttcaca tattcgccgg
    12601 tcgccaaatc gaccacgcgt tgctcgatac gctcggcaaa ggttttgccg atgtcggtgt
    12661 ccttatccat caaaccggcc acgctcaaaa tatagccttg tttgtcttgg agtttttgtt
    12721 tttcttggat gggtttcaag ccgacgaccg caccggcaac gatgaccgag caaatcaggc
    12781 tgaccgccaa cacgacaatc agcgtgccgc tgaagctgtc tttatcgaat ttcttagcca
    12841 ttgctgcgcg cctttctgcg tttgatgttc gcttgtgcga cgaaatagtc gaaaatcggg
    12901 gcaaacaggt tggcaaacag aatcgccaac atcatgcctt cggggtaagc ggggttgacc
    12961 acacggatca atacgcacat cacgccgatc agcgcgccgt accaccattt gccgacattg
    13021 gtaaaggagg cggaaacagg gtcggtcgcc ataaacagca taccgatggc gaagccgccg
    13081 acgaccagat gccagtacca aggcatagca aacatagcgt tggtgtccga accgatgaag
    13141 ttgaacagcg aagacatggc aatcataccg atcatcacgc cggcaataat gcgccaagaa
    13201 gcgatgcggg caaacacgat aaacgcgccg ccgattaaga gcgccaaagt ggagacttcg
    13261 ccgatggagc cgggcagttt gccgataaac gcgtccatcc aagtgatggt ttgaccggtt
    13321 acggcgtttt tcaggccgtc tgcaccgtgt gccgcccatt gcgccagcgc ggttgcaccg
    13381 gaatagccgt caaccgccgt ccaaaccgca tcgccgctca agttggctgg gtaggcgaag
    13441 aacaggaagg cgcggcctgc cagcgcaggg ttcataaagt ttttacccgt accgccgaat
    13501 acttcttttg caaccaccac gccgaaggta atacccaagg cagcctgcca cagcggcagc
    13561 gtgggcggaa cgattaaggc aaacagaatc gaagtcacga agaaaccttc gttgatttcg
    13621 tgtttgcgca cggtggcgaa caagacttcc caaaaaccgc ccacgacaaa tacggtcgcg
    13681 taaatcggca gaaagtaaat cgcgccgaac agcattttgc ccgacacacc cgcttcagac
    13741 gacatattga tgcccaaagc gtcggcaagg gtgtaatgcc agtcgttggc gatgctttgt
    13801 tgcagcaaat cgggcgttaa ggcaccgaat gcctgtgcgc caacgttgta catgccgtaa
    13861 aacatggcgg ggaacaaagc cagccacacc aaaatcatca tgcgcttgga gtcgagcgcg
    13921 tcgcggacgt gtgccgcttt gcgcgttacc gcaccggatg tgtagaaaat cgtcgccgcc
    13981 gcttcgtaga gggcatacca tttttcatgt ttgccgcccg gcaggaagtg cggttcgatt
    14041 ttttccagaa aatgtttcaa gcccataatc agccttcctt ctcaatggtt tccagcactt
    14101 tgcgcaacag cgggccgtat tcgtatttgc ccgggcagac gaagctgcac aaagcgaggt
    14161 cttcttcgtc caattccaag caacccaatg cctgcgcgct gtcggtatcg ccgacgatta
    14221 aatcgcgcaa aagcagggtg ggcaggatat ccaagggcat cacgcgctcg taagtaccaa
    14281 tcggcaccat ggcgcggtcg ccgccgttga cggctgtgtt gaacttgaag agtttgtttt
    14341 tcaggaaatg gccgagggtt gtacgcgtga tggagtattt gtccggctgc ggcgcaaccc
    14401 agccgaacag ctctttgctg cggccttctt cgataacgga aatctgattg tggtagcgtc
    14461 ccaaataatc gtgcgcgcct tgtgcaatcg cgccgttcaa taccgaaccg gaaatcacgc
    14521 ggttgtctgc gtcaaccaat tcgcccgcag taatttgcga tactttcgca cccaaaacgg
    14581 tacgcaagag gcgtggtttg ttgacttgag aaccacccaa agcaatcacg cgcccggtgt
    14641 tcagacggcc tgttgcaaac aaacgtccga tggcaatcac gtcttgataa ttgatggtcc
    14701 aaacggtttt gttcgcaccg accggctcaa tgaaatgaat gtgcgtgcca ctcaaaccgg
    14761 ccggatgcgg gccgccgaat tcatgtgttt cgatgttggc agcattttca gacggcacgt
    14821 ctgcgccagc tgccttacaa acatggattt tgcgttcggt caaacggctc aataccaaca
    14881 ggccgcgttt gaaatcctcg gcggcttctt tgataatgac cgtagggtcg gcagccagcg
    14941 gattggtgtc catcgcattg acgaagatgg cgaacggctc ggcatcgacg gcaggaattt
    15001 tgctgaacgg acgggtgcgc agcgcagtcc acaaaccgga ttggatcagg ttgcggcgca
    15061 cttcttcgcc gcttaagttt gccagcgctt caggtgcgta gcgttcaaac tcgatttcgt
    15121 cgttgccttc aacggcaatc acgactgact gaagtacgcg cttttcgcca cggtgaatcg
    15181 cggcgatttt gcctgaagcc ggcgcagtaa acaccacgcc cggattcttt ttgtcttcaa
    15241 acagcacttg gccttttttg acggcatcgc cttccttgac tttcatcgag gggcgcatac
    15301 cggcatattc ttcgccaagc aacgcgactt cggtaatgac gggcccgtca taaatgactt
    15361 gctccggtct gcccgcgatg ggcaggttta gacctttttt gattttaatc atatatttgc
    15421 attacttgtg atggttaagg taaaaacggc gtgttttgat accgtgtcgc gtggcatcaa
    15481 aagcattgaa taaattaatg tagcaaagtg ttagattcta tcaggaattg tacctgtttg
    15541 tcagatttgc tgcttttttc cttgcggaag ctg
//
LOCUS       DAWFLT010000043        15492 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWFLT010000043 DAWFLT010000000
VERSION     DAWFLT010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15492
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.043"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            86..1093
                     /locus_tag="WGR74_001315"
     CDS             86..1093
                     /locus_tag="WGR74_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ0905730.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPAGDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYVPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNMLSIGQIVKVKPAGYAAPKTSAVE
                     SRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAENKAVPAPAPQSPAASPSGTRSVGGI
                     VWQRPTQGKVVADFGGNNKGVDIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQHN
                     SSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSYIA
                     F"
     gene            complement(1300..1782)
                     /locus_tag="WGR74_001316"
     CDS             complement(1300..1782)
                     /locus_tag="WGR74_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0905731.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(1935..2096)
                     /locus_tag="WGR74_001317"
     CDS             complement(1935..2096)
                     /locus_tag="WGR74_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905732.1"
                     /translation="MSGQPEKHHTSPIEDERKNPVYRMGRAVAGFMLVVWAGVLALVF
                     FLVFRFWLS"
     gene            complement(2170..2631)
                     /gene="recX"
                     /locus_tag="WGR74_001318"
     CDS             complement(2170..2631)
                     /gene="recX"
                     /locus_tag="WGR74_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ0905733.1"
                     /translation="MKPQKSLRARAMDILSRQELSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKLAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(2699..3406)
                     /locus_tag="WGR74_001319"
     CDS             complement(2699..3406)
                     /locus_tag="WGR74_001319"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ0905734.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETA
                     TGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKEE"
     gene            3813..5078
                     /locus_tag="WGR74_001320"
     CDS             3813..5078
                     /locus_tag="WGR74_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ0905735.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDGTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGITLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITNE
                     AGYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(5136..5942)
                     /locus_tag="WGR74_001321"
     CDS             complement(5136..5942)
                     /locus_tag="WGR74_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ0905736.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            6287..6496
                     /locus_tag="WGR74_001322"
     CDS             6287..6496
                     /locus_tag="WGR74_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ0905737.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            6585..7799
                     /locus_tag="WGR74_001323"
     CDS             6585..7799
                     /locus_tag="WGR74_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ0905738.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKSYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVSQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(7886..10465)
                     /gene="clpB"
                     /locus_tag="WGR74_001324"
     CDS             complement(7886..10465)
                     /gene="clpB"
                     /locus_tag="WGR74_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ0905739.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHSGVNVPQVKQRLQQHLSSLPKVSGQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALIQQNDATGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQDNIRNIAKIQLKGLEKRLEKQNLRLAV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            10726..11736
                     /gene="trpS"
                     /locus_tag="WGR74_001325"
     CDS             10726..11736
                     /gene="trpS"
                     /locus_tag="WGR74_001325"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ0905740.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFRELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETVEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            11742..12083
                     /locus_tag="WGR74_001326"
     CDS             11742..12083
                     /locus_tag="WGR74_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905741.1"
                     /translation="MPSEPLKGLPEQNVVRLIGKHTNDLEAVVGKCMETDGKGAPSGW
                     AANGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            12404..12748
                     /locus_tag="WGR74_001327"
     CDS             12404..12748
                     /locus_tag="WGR74_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905742.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAKEAVTEAAKDTLNKAADAT
                     QEAADKMKDAAK"
     gene            complement(12957..14465)
                     /gene="ppx"
                     /locus_tag="WGR74_001328"
     CDS             complement(12957..14465)
                     /gene="ppx"
                     /locus_tag="WGR74_001328"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ0905743.1"
                     /translation="MTTTPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQAAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALSERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMGGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(14581..15313)
                     /locus_tag="WGR74_001329"
                     /pseudo
     CDS             complement(14581..15313)
                     /locus_tag="WGR74_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
BASE COUNT         3499 a         3884 c         4229 g         3880 t
ORIGIN      
        1 gtcgggcttt gcgcttgaga agaacgcgca tttttattat gatataccgc attcaggcta
       61 tatatcggaa catgaaggtt atcttatgtt gaaacaaacg acacttttgg cggcttgcgc
      121 ctcctttgcc gcactgttgg gcggttgcgc cacccaacag cctgctcctg tcattgcagg
      181 caattcaggt atgcaggacg cgccgtcttc ggcagtttac aacaacccct atggagcaac
      241 gccgtacagc ccggctcctg ccggcgatgc gccttatgtg ccgccggtgc aaagcgcgcc
      301 ggtttatacg cctcctgctt atgttccgcc gtctgcacct gccgtttcgg gtacatacgt
      361 tccttcttac gcacccgtcg acatcaacgc ggcgacgcat actattgtgc gcggcgacac
      421 ggtgtacaac atttccaaac gctaccatat ctctcaagac gatttccgtg cgtggaacgg
      481 catgaccgac aatatgttga gcatcggtca gattgttaaa gtcaaaccgg caggatatgc
      541 cgcaccgaaa acctcagccg tagaaagcag gcccgccgta ccggctgccg tgcaaacccc
      601 tgtgaaaccc gccgcgcaac cgcctgtgca gtccgcgccg caacctgccg cgcccgctgc
      661 ggaaaataaa gcggttcccg cgcccgcccc gcaatctcct gccgcttcgc cttccggaac
      721 gcgttcggtc ggcggcattg tttggcagcg tccgacgcaa ggtaaagtgg ttgccgattt
      781 cggcggcaac aacaagggtg tcgatattgc aggaaatgcg ggacaacccg ttttggcggc
      841 ggctgacggc aaagtggttt atgccggttc cggtttgagg ggatacggaa acttggtcat
      901 catccagcat aattcttcct tcctgaccgc atacgggcac aaccaaaaat tactggtcgg
      961 cgaaggtcag caggtcaaac gcggtcagca ggttgccttg atgggcaata ccgacgcttc
     1021 tagaacgcag cttcatttcg aggtgcggca aaacggcaaa ccagttaatc cgaacagcta
     1081 tatcgcgttc tgactttctg cctgaaatca agttggaaat gccgtctgaa aggttttcag
     1141 acggcatttt tgtttttggc ggctgtgttt tcaaatcatt cggaagggac ggggcatata
     1201 aatttgtttg actgtgtttg ggtatttgct tgatttcatc cgtttgcagt agaaacggat
     1261 ggcgcgatgt gcctgtcttt cagacggcat agtccgccgt cagcagccgc aggacatgtc
     1321 ttcagaggct tgcaggctga tgtctctgcg ttttttggct ttttcgtagc ggcagcgttg
     1381 gcggtcggtc aggatttcca gcggagggac agggacgggt ttgccgtctt tgaccgcaac
     1441 catcgtgaag tagcagctgt tggtatggcg gatttcgccg gtgcggatgt tttgtgcttc
     1501 gacacggatg ccgatttcca tcgaggtgcg tcccgtgtag ttgacagcgg cgtaaaaggt
     1561 aaccaaatcg ccgatgtgga tgggttcttt aaacaggact ttgtcaaccg acagggtaac
     1621 gcaataattg ccgctgtaac ggctggcgca ggaataggcg acttggtcga gcaggagcag
     1681 gagttcgccg ccgtgtacgt tgccgctgaa attggcggtg tccggcatca tcagttcgga
     1741 cataatgagt tcgtgggaag gcagttggcg ttgttgtgtc atagtgtacc tgttttaaga
     1801 aagggtgttt tgaaatgggt gcggggaaat tttagggaat ggggaagtgg aattcaagct
     1861 aaaaaaagct atgatgggat agtctgccga aatggaccgc cgatgccgtc tgaagccttc
     1921 agacggcatt ttgtttagga aagccaaaaa cggaagacca ggaaaaacac gagtgccaat
     1981 acgcccgccc aaacgacgag catgaatcca gcaactgccc gtcccatacg gtaaaccggg
     2041 tttttgcgtt cgtcttcgat gggggaggtg tggtgttttt cgggttgtcc ggacattttg
     2101 ttctcaatcg gaaaagttgg aaacgggcgt aaggttatgt tgccgtgcaa aaagattcaa
     2161 gaaggaaggt cagcagccgt cttcccaatt ttcgtcccaa gcgtgtttca atgccgtctg
     2221 aacggtatcg gcatcgaatc cgcgataggc gaggaaacgc gcctgttttt gtttttcttt
     2281 gaggtcggcg gcggggtgtt tgaatttttt acgcaacacg gctatggcgg ccagtttttc
     2341 gcttgagcgg tcgggaagca ggttgcggct ggtttcttca tctatgccct gttgcgccaa
     2401 agcctgtttc agcctcaatg aaccgtgttt gcggcttttg ctgcggatat aggcttcggc
     2461 atagcgcaaa tccgactgcc agttgcgttc ggcaaattcg tttaacacgt tttccaactc
     2521 ctcttcgctt tcggcgtgcg gtgcaagttt gcgtttcaga ccgatgcggc tgagttcttg
     2581 gcgcgagagg atgtccatcg cgcgggcgcg tagggatttt tgcggtttca tggcggcggt
     2641 ttcggcgtgg ggcaggtttc agacggcata gcggcgcaaa ccggagccgt ccgaatgcct
     2701 actcttcttt gtttttctga ggttgcaggt tttcgtaaat ttcgggcagc gagccgataa
     2761 tccagtcggg gcgggtcgca tcgtcttgcg agagcagcgt catatcgccg taaccgaagg
     2821 taacgccgac gctcaggcag ccggcggctt tggcggcgat gatgtcgttg cgcgagtcgc
     2881 cgaccataac catgtttgca acatcgatac ccaaaacttc ggcggcgtgc cgcagcggca
     2941 gggggctggg ttttttctcg ggcaggctgt cgccgccgag tatcaggctg aagtagtcgg
     3001 caagtccgag ctgtttcaac agttcgacgg caaggatttc gtttttattg gtaatgactg
     3061 ccagcgggat gcccaaagat ttgagcaatg ccagcccggt tgcggtttcg ggataggggc
     3121 gggtgaagac gctcaaatgg tcgcggtagt atttcatata ggatacgaaa cctttttccc
     3181 acagttcgga atcggcttcg cggtcgcggt cgttggtgag gacgcggtga accagtttgc
     3241 cgatgccgtc gcccacatag ctttcgacca ctttggcggg cagcggtttc ataccgagtt
     3301 gttccaacat cgcttctgcg gcggcggcaa ggtcggggac ggaatcgcac agtgtgccgt
     3361 ccaaatcgaa ggcgacggct tggacgtgtt cgatggctgc attcataatc tgatttcctg
     3421 tcgggttgaa agggtttatt ttaacatcgt ttgcacaggg cttctaatgt atagattgtt
     3481 aacaatcgca acaggttttg caggcggtgt tttcggattt cggggcggaa tcgggcaagg
     3541 gtttgaaatc ccagcgatgt tccaattaaa tttttaaaaa ataaaaggat gcctatatct
     3601 ttatttaatt tgacatttat ttttcggatg cgcgcgctgc tttttcaggc ggagtccggg
     3661 ctgattccgt gcggcttgaa aaataaaatc atatatagat taatcaaatc gggcttatgc
     3721 cgactacacg ctattacatt ttgtcgggaa attggttata gtaatgagcg aaatgcacgc
     3781 ccctgaaaat ctttttgtga aaaggaagca aaatgtctga agcccttgca aaagaaaccc
     3841 tcaatccgtt tgaaatcgcg cgcaaacagg tcaaaaccgc ctgcgaccgg ctgaaaaccg
     3901 atccggccgt ttatgaaatc ctgaagagtc cgacccgcgt gttggaagtc aatttccccg
     3961 tcaaactcga cgacggcacg gtcaaaacct tcacgggcta ccgttcgcaa cacaacaacg
     4021 ccgtcggccc ctataaaggc ggcgtgcgct tccatcccgg tgtgaatctg gacgaagtca
     4081 aagccttgtc aatttggatg accatcaaat gctgcgttgc gggtattcct tatggcggcg
     4141 gcaaaggcgg cattaccttg gatccgcgcg attattccga ggcggaactg gaacgcatcg
     4201 cccgcgctta ttccgaagcc atttctccgc tgatcggcga gaaaatcgat attcccgccc
     4261 ccgatgtgaa caccaacggc aaaatcatgt cttggatggt tgatgcctat gaaaacgtgg
     4321 tgaaacattc tgcaccgggc gtatttaccg gtaaaccggt tgagttcggc ggctctttgg
     4381 cgcgtaccga agccacgggt tacggcgtga acctcgccgc cgtccaagcc ttggaaaaac
     4441 tgggcaaaga cgtgaagggt gccacttacg ccattcaagg tttcggtaat gtgggttacc
     4501 acacaggtta ttacgcgcat caatccggcg cgaaagttgt tgcggtttct actgtcgatg
     4561 ttgccattta caacgaaaac ggtttggata tggaagccct gttcaaagag ttccaagaaa
     4621 aaggcttcat caccaacgaa gccggttacg gcaaggaaat taccaacgcc gaacttttgg
     4681 ctttggatgt ggacgtactc gccccttgcg cgctggaaaa tcaactgact tctgaaaacg
     4741 ccggtaaagt ccgcgcgaaa atcgtggtcg aaggcgcgaa cggcccgact acgcccgaag
     4801 ccgacgtgat cctgcgtcaa aacggcgtat tggtcgtgcc cgatattttg gcgaactgcg
     4861 gcggcgtggt ggtatcttat tttgaatggg tacaaaatct gcaaggctat tattgggagt
     4921 ttgacgaagt tcaggaaaaa gaaaccgtcg tcctgcgccg tgcgttccgc gatatatgga
     4981 acttggcgca agaatacgat gtcgatttgc gaaccgcgtc ttacatgatg agtatccgcc
     5041 gtgttgaaaa agcgatgaag ctgcgcggtt ggtattgatg tttgaacaaa aaatgccgtc
     5101 tgaagccttc agacggcatt tttgtgcagg cgggattaat ggtgcgaatg gccgatttgg
     5161 aaggttaaag tcgagtagtt cgcctgtttt tggcacacgc tttgatcggg gaagtcggct
     5221 ttgtgttcga cattggcttt ccagaagcct tggcgcaagg ggatgatgtc cacttcgcct
     5281 ttgtcgtctg tgctgtcgga gaaagcctgt gcttcggttt tgtgcgtttt gctgcggtcg
     5341 ctggtgtcga agccgtcaaa ggtggcggta acggtggcat tgggcagcgg ttcgccacgg
     5401 aacagaacgc ggactttgaa gcgttcgcct acgtgaatgt tggcgggatt gtccagcggg
     5461 acgatttcca agttttgtcc gaccggtttg gtgatgatgg cggtgtccgc gctttcgtgt
     5521 ccgacgttga cgatgttttt gccgaacatt cgggtttgtt cgcaatagct tgcgtcaggc
     5581 atttctttga tgcccgcctg tttccagcct gctttgtttt ttgaccagaa agtaggctga
     5641 tattcggcga tgacgaggta actgccgtcc ttaacgggac ggttgcttcg gtactggtag
     5701 ttgtatgtgc cgcgttgaat catgttttcc ttgccttttt cggtaaccag ctgcatcggt
     5761 ttgctgaaga tgtgcaggcg gtctttggcg atgggttcga gttcgggaaa ttcgccgtag
     5821 cccaagtcgg ctttaaggta ttcgccgccg tgcgtgtggg cggtttcgac ccagacgcgg
     5881 tgggcgtggg cggatgtggc aaatagggcg gaaacggcga gcagtgtcaa tgttttcttc
     5941 atggtgcagc ctttgtctcg tgtgggtcaa agattttgtt atgttataac aaaataggta
     6001 tcgtgtgaag acggatttgc tgtctgaaaa ctgtcttttt gtatagtgga ttaacaaaaa
     6061 ccagtacggc gttgcctcgc cttagctcaa agagaacgat tctctaaggt gctgaagcac
     6121 caagtgaatc ggttccgtac tatttgtact gtatgcggct tcgtcgcctt gtcctgattt
     6181 ttgttaatcc gctatatcga tttgaatttt tcagaaaatg aagcggacgt ttgggcggag
     6241 gcaacttgtt tgataagata gcaatatttc aaaacggaga aagatcatgc cttatgtcaa
     6301 tattaaagta accggcggca aggaagcacc gactgccgcg caaaaagcgg aactgatcgg
     6361 cggcgtaacc gaattgctgg cacgcgtgct gggcaaaaat cccgaaacaa cggttgtcgt
     6421 gattgatgaa gtggataccg ataactgggg aataggcggc aaaagcgtca gcgaacggcg
     6481 caaagagggc aggtaaaaag cctgaaaatc tcggtttgat gctttaaatt ccgcgtgaaa
     6541 aagagtacat tcccacccat tgcccaaaat ttacggaaca catcatggat aaatttccca
     6601 agtctgcaaa gctcgatcac gtctgttacg acatacgcgg gccggttcac aaaaaagccc
     6661 ttcagttgga agaggagggc aataaaatcc ttaaactcaa tatcggcaac cctgcgccgt
     6721 tcggctttga agcccctgat gaaatcttgg tcgatgtcat ccgcaacctg ccgacttcgc
     6781 aaggctattg cgattctaaa gggctgtatt ccgcccgcaa agccattgtt cactactatc
     6841 agaccaaagg tttgcgcgat attacggttg atgatgtcta tatcggcaac ggcgtgtccg
     6901 agctgattac gatgtctatg caggcattgc tcaacgacgg cgacgaaatc ctgattcccg
     6961 cgcccgacta ccctttgtgg acggcggcgg caacgcttgc gggcggtacg gtacgccatt
     7021 atctgtgcga cgaagaaaac ggctggttcc ccaaccttgc cgatatggaa gccaaaatca
     7081 cgcccaaaac caaagccatc gtcgtcatca atcccaataa tccgacaggc gcggtgtaca
     7141 gcagggaaat cctgttggaa atcgccgaac tggcgcgcaa gcacggtttg attattttcg
     7201 ccgacgagat ttacgataaa atcctttatg acggcgcggt tcaccaccac atcgccgcgc
     7261 ttgcccccga ccttttgacg gtaacgttca acggtttgtc caaatcctac cgcgtcgccg
     7321 gcttccgcca aggctggatg gtgctcaacg ggccgaaaca tcacgcaaaa ggttacatcg
     7381 agggtttgga tatgctctcg tctatgcgcc tgtgtgccaa tacgccgatg cagcacgcga
     7441 ttcagacggc attgggcggc tatcagagca tcaacgaatt tattttgccc ggcgggcggc
     7501 ttttggaaca gcgcaacagg gcgtgggaac tggtcagcca aatccccggc gtgtcctgcg
     7561 tcaaaccgat gggtgcgatg tatatgttcc cgaaaatcga taccgaaatg taccgcatcc
     7621 gcgatgacat gaaattcgtt tacgatttgc tggtgcgcga aaaagtcttg ctggtacagg
     7681 gaacggggtt taattggatc aagcccgacc atttccgcat tgttacgctg ccttacgtcc
     7741 atcagattga agaggcgatg ggcaggctgg caagattcct gcaaacctac cgccaataag
     7801 gggacgggct gtctgccgag gataaaaaat gccgtctgaa acggagattc ccgtttcaga
     7861 cggcattttt aacagcagga acgaatcagg caaatttcag tctgtcgccg tcggcttcca
     7921 ccctgatttc gctttcgggc gcatagtttc cggcaagcag ggcttttgcc agcgggtttt
     7981 cgatttccga ctggatggcg cgtttgagcg gacgtgcgcc gtaaatcggg tcgaaaccgg
     8041 ctttggcgat gatgtccagt gcggcatcgg aaacagccag gcgcaggttt tgtttttcca
     8101 aacgtttttc caagcctttg agctggattt tcgcaatgtt gcggatatta tcctgatcca
     8161 gtccgtggaa cacgaccact tcgtcgatgc ggttgatcat ttcggggcgg aaatgttctt
     8221 tcacatcttc catcacaact tcttttaccg cttcatagtc ttgagtaccc atttgttgga
     8281 tatgttggct accgatgttg gaagtcatca caatcacggt gtttttaaaa tctaccgttc
     8341 tgccctgtcc gtcggtcaaa cgaccgtcat ccaatacttg cagcaggatg ttgaacacat
     8401 cgggatgggc tttttccact tcgtccaaaa gaattacgct gtacggtttg cggcggactt
     8461 gttcggtcag gtagccgcct tcttcgtagc cgacatagcc tggaggcgcg ccgatgaggc
     8521 gggcaacggc gtgtttttcc atatattcgg acatatcgat acgaatcaga tgatcttcac
     8581 tgtcaaacag gaagcctgcc aatgctttgc acaactcggt tttacccacg ccggtcgggc
     8641 ccaagaacag gaagctgccg taaggtttat tcgggtcggc aagaccggag cggctgcggc
     8701 ggatggcgtc ggacacggca cgcacggctt cgtcttgacc gactacgcgg cggtgcaaca
     8761 cttcttccat tttcagcaac ttgtcgcgtt cgccttccat catttttgac acaggaatgc
     8821 cggtcatacg ggaaaccact tctgcgattt cctctgcgcc gacattatta cgcaagagtt
     8881 tgtttgccgg ttttgtgctg tccgtatctg cccgttcggc ggctgcacgc tgtttttcca
     8941 aatgctccaa atcttcatac atcaattttg aagccagtgc caaatcgcct tgccgttttg
     9001 cctgttcgat tttaattttg acttcgtcaa tttgtttctt aatattagca gcaccgtctg
     9061 aaattgcttt ttcggctttc cagatttcgt ctaaatcggc gtattctttt tgcagaccgt
     9121 tgatttcctc gtctatcagt tccaaacgtt ttttgctggc atcgtctttt tctttttcaa
     9181 cgtgcgcctt ttccatccga agctgaatta gacggcggtc gattttgtcc attgcttccg
     9241 gcttggtttc tttttccatc ttgacacggc tggcggcttc gtcaatcaaa tcaatcgctt
     9301 tatcaggcag gaagcggtcg gtaatataac ggtcgctcaa ctccgctgcg gcaacgatag
     9361 cagggtcggt aatatcgata ccgtggtgga tttcataacg ctcctgtaaa ccgcgcaaga
     9421 tagcgatggt gtcttccaca ctgggttcgc cgaccaatac tttttggaaa cggcgttcga
     9481 gtgccgcatc tttttcgatg tattggcggt attcgtccaa ggtggtcgcg ccgatgcagt
     9541 gcaattcgcc gcgtgccaaa gccggtttca acatattgcc cgcgtccatt gcgccgtcag
     9601 ttttgcctgc gccgaccaaa gtatggattt catcgatgaa aatcagggtg ttgccgtcgt
     9661 ctttcgccaa atcgttcaat acgcctttca agcgttcttc aaattcgccg cggtatttcg
     9721 cgccggcaat caaagccgcc aaatccaaaa ccagcaggcg tttgttacgc agggattcag
     9781 gtacttcgcc gttgacgata cgttgcgcca agccttcaac aatagcggtt ttacccacac
     9841 ccggctcacc aatcagcaca gggttgtttt tggtacggcg ttgcaatacc tgaatcgcgc
     9901 ggcggatttc gtcgtcgcgg ccgataacgg ggtcaagttt gccgtcgcgg gcgcgttgag
     9961 tcaggtcaag tgtgtatttt ttcaaagcat cgcgttggtc ttcggcattc gcatcattca
    10021 cgttttgtcc tcctcgtacc gcgtcaatcg cggcattgat attttgttcg gtcgcgccgg
    10081 cttctttcaa aattttgccg gtcgcatcgt tctgctgaat caaggcaagc aggaaaagtt
    10141 cgctggcgat ataggcatcg ccacgtttgg ttgctgcttt gtccatcagg ttcaacaccg
    10201 cctgcaattc gcggctgggc agaatatcgc cgccctgacc ggacactttc ggcaggctgc
    10261 tcaaatgctg ctgcaaacgc tgtttcacct gcggcacgtt cacgcccgaa tgcgccagca
    10321 acgcggcggc tccgctgttt tggtcgtcaa gcagggcttt taacacaaag cctgcttcta
    10381 gatagctgcc gtccgcagcc aacgccaaac tctgagcttc tgcaagggct tgttggaatt
    10441 tggcggttaa tttgtcgtat cgcatttttg tttccttttc aaaatgtccg ctgtcgaagc
    10501 ctatatgtgc ataattgtgg ataactcaag ttctgttttc tgtttttcta tatttaattc
    10561 gatatatcat tgaatttaaa gtatataaaa atgtataata atgtgtataa ctatatcttc
    10621 ttaatatgga aaagtctgtt gtcggctgga tgtaggtggc aaatcgggta taatcggcgc
    10681 atctttttcc ctttcagacg gcattgatgc cgcaaggaca tttttatgag caaaaaacga
    10741 gttctgaccg gcgtaaccac caccggcatc ccgcatctgg gcaactacgt cggtgctatc
    10801 cgccccgccg tccgcgcggc gcaaaaccct gataccgaat ccttcctctt cctcgccgat
    10861 taccacggta tcatcaaatg ccacgagccg gagatgattc accaatccac ccaagccgtt
    10921 gccgccactt ggcttgcctg cggactcgac cccgagcgca ccaccttcta ccgccaaagc
    10981 gacattcccg aagtgatgga attgaactgg attctgacct gcatcaccgc caagggtttg
    11041 atgaaccgcg cccacgccta caaagccgcc gtgcaggcaa atgcggaaaa cgggcaggaa
    11101 gaccccgatt tcggcgtgga aatgggtttg ttcagttatc cgattctgat gactgccgat
    11161 attctgatgt tcaacgccaa cgaagtcccc gtcgggcgcg accaaatcca acacgtcgaa
    11221 atggcgcgcg acatcgccgg ccgcttcaat caccgcttcc gagaactctt caccctgccc
    11281 gaagtgaaaa tcgacgaaaa cgtcgaactc ttggtcggtt tggacggacg caaaatgtcc
    11341 aaatcctacg gcaacaccat tccgctttgg gaaaacgaca aaaaaaccca aaaatcggtc
    11401 aacaaaatca tcaccaatat gaaagagccg ggcgagccga aacagcccga cgaaagccca
    11461 ttgtttgaaa tctacaaagc cttctccacg ccgtctgaaa cggtggaatt tacgaaaatg
    11521 cttgccgacg gcctggcttg gggcgaggca aaaaaacttt tggcggcgaa aatcaacgcc
    11581 gaactcgccg aaccgcgcga acgctacaac gagctgaccg ccgacccttc gcaaatcgaa
    11641 gagattttac aggcaggtgc gcaaaaagcg cgtaaagaag cacgcgaatt gttggacaaa
    11701 gtacgcgatg cggtcggcat ccgcccgttg aaataaaccc gatgccgtcc gaacctctta
    11761 aaggcttgcc ggaacaaaac gtcgtccggc tgatcggcaa gcataccaac gacttggaag
    11821 ccgtcgtcgg caaatgtatg gaaaccgacg gaaagggcgc gccttcgggc tgggcggcaa
    11881 acggcgtgtg ccataccttg tttgccaaac tggtaggcaa tatcgccgaa gacggcggca
    11941 aactgacgga ttacctgatt tcgcattccg ccctgcaacc ctatcaggca ggcaaaagcg
    12001 gctatgccgc agtgcagaac ggacgctatg tgctggaaat cgacagcgag ggggcgtttt
    12061 atttccgccg ccgccattat tgaggtattc ggacatcccg gaatatattt gggtttttca
    12121 aaccctgcag gaaaaagtcc gcaccgacga gaaacgcaaa aggaggggga tatgtgttac
    12181 aattttccga actgttttca taaaatagtt ttcggacgtg tttcaatatg gcattgatgc
    12241 cgccttattg ttcgagaaaa aaaaccttta tatttaaata taatgggttt taactaaacg
    12301 ggaaaccgtt ttctctccgg ccgatgggca aaatcagccg attgatggaa cacggtccga
    12361 tttttaagca aaaccttaat caacaactca agaaggataa gacatgaaaa aattattgat
    12421 tgccgcaatg atggcggctg ccttggcagc ttgttcgcaa gaagccaaac aggaggttaa
    12481 ggaagcggtt caagccgttg agtccgatgt taaagacact gcggcttctg ccgccgagtc
    12541 tgccgcttct gccgtcgaag aagcgaaaga ccaagtcaaa gatgctgcgg ctgatgcaaa
    12601 ggcaagtgcc gaggaagctg taactgaagc caaagaagct gtaactgaag ccaaagaagc
    12661 tgtaactgaa gcagctaaag atactttgaa caaagctgcc gacgcgactc aggaagcggc
    12721 agacaaaatg aaagacgctg ccaaataatt tgttgctttg gcaaaaatga cgagatgccg
    12781 cctgccggca acccaaacga aaccgcctga agattttcag gcggtttttg agttgtatgc
    12841 cccttgtttt ttacgcctgc ataaccgttc cgagcagatg tgccgcgcat atcgggatgt
    12901 tgcggacagg gaaatgccgt ctgaacatca ttcagacggc atttgccccg caaggttcag
    12961 acggcctcga ctttgaacgg catattgatt ttttgccatt ggacgctttc atattccaat
    13021 gcgtcggcaa tcaggggatg gcgttccagc cattccctgt caatacgcag gatgaagccg
    13081 caactttccg tgtccgtacg caactgcata tttttcggga aagacaggtc ttggcgcgaa
    13141 cggcagaaca gggcggcaag gcgcagcgac aaaacggcat accacaacat ttcgttggtg
    13201 ccgataatgc cgcccatttt tttcatatcg ccgcgatgac cgatgacgag ctgggcgagt
    13261 atggtctgtt ctttgcgtga gaaacccggc atatcggcgt tttcgaggat gtaggcggaa
    13321 tgcttgtgat agccggtgtg ggcgatgtcc aaaccgattt cgtgcagcgc ggcggcgcgt
    13381 ccgagatact gttgccacaa ggcaagctct tgaactgtaa cgtttttagc gtggcagagg
    13441 ctgtccataa aggtttgcgc ggtctcggcg gtgcgtttcg cctgattgag gctgacgtgg
    13501 tagcggtgtt ggaactcggc aaccgtttgt ccgcgcatat cttcgtttaa accgcgcccg
    13561 atcaaatcgt aaaacacgcc gtcgcgcagg gcggcttcgg ttacggtcat cctgtcgagt
    13621 ttcatttcct caaacgccgc catcatcacg gcaagtccgc cggcaaaaac ttcgatgcgt
    13681 tccggtttca ggttttcaaa tttggctttt ttgaccgaac cggcttcgat gatgcgttcg
    13741 gagagggcgc gcataccttt gtaggtaatg tccgcctctt ggggcatttc ggcggcaagc
    13801 acgtcgcgga tggattttgc cgaacccgat gtgccgacgg cgaaatccca gccttcgcgc
    13861 ctcatatttt tgctgatgcg ctggatttcg ttgcgggcgg cggaaatggc ggcttggaag
    13921 tctttggcgg tgattttgtt ttggaagaag cgcaggctgt aggttacgca gcccaagggc
    13981 aggctttcgg taatgtcggg attcagcgtc gaaccgatga caaattctgt cgaaccgccc
    14041 ccgatgtcga taaccagcat tttgccgccg ccggggggga gggtgtggat cacgccggta
    14101 taaatcagcc gtgcctcttc gcgcccggcg atgatttcga tggggaaacc cagtgccgct
    14161 tcggctttgg gaaggaaatc tgcgatgttt ttggcgacac ggaatgtatt ggttgccacg
    14221 gcgcgtacct gttcagggcg gaagccgcgc aggcgttcgc cgaattttgc cagacagtcc
    14281 aaagcctgtt cttgggaagc ggcactcagg tttttctgtt cgtccagtcc ggcggcgaag
    14341 cgcaccatct gtttgaacga atcgatgact ttcaactgtc cgttgttgtt ttcgcaaatc
    14401 tggaggcgga aactgttaga acccaaatcg acggaggcaa ggacgtttgc gggggtggtg
    14461 gtcatggcgg ataccggtgg gggaaaaacg caatgttact ctgacggcgc aggcgttgac
    14521 aataaatgat gcggcggttt ttgattctgc ccacggatgt tgccgacggc atttttgcgc
    14581 ttatttgaaa tccttttcca cgctcatgaa aatctgcgtg tttttgcgtg tgtaaaaact
    14641 tttcatattg ctgtcgattt tcagatagcg gaaattgagt tgcggcgtaa agcccttcca
    14701 agagattttg tcgtgccaca acgacaggtt tgcctgatat tcgtggtctt tgcgcgggaa
    14761 gcggtacaca atggtcccgg gtgcgtcaaa catcctgcgg gtatagcgca ggtttgcccg
    14821 cagacccaag cccgccgtcg aacgttttga ctgcgccgac acgcaaaccc ttgcggacgg
    14881 aagcctgttc cgcctctttc gtcatgttgt gcgaccagtc cgcaccgccg taaagcagcc
    14941 agtctttcgg cgcggaatac atcagcgtcg cgcccgccag cggcatatgg ctgtcgtatc
    15001 gggcggcggt gcggtcttcc tgataacgct tccacatatt gcccgcgttt agtgtcaacc
    15061 gccagcgttc gcccaagcgt tgggagaaat cggcattgaa gccgccgacg aaattgtatc
    15121 ggctgccgcc taagaggttt tgcccgacaa acggcacgat gccgaacgag cgcgttaccg
    15181 aacgattttt atagccgagc gacaggtgca ggcactgttc gctgaaatct ttgttatccc
    15241 aataatgcac gccgccgccg ctgatgccgc cgtagaggaa atgatgcccg cccgcattga
    15301 tttcgcgcga cacgcctaag ccctagcgca aaccgtgtgc cttttgcggc aggctgtcgg
    15361 cggttttcgt ccagcttctg cccgcaaatt caatcgtttt tttggacgaa gcgttgttta
    15421 tagcggatta acaaaaatca ggacaaggcg acgaagcctc agacagtaca gatagtacgg
    15481 aaccgattca ct
//
LOCUS       DAWFLT010000044        15315 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWFLT010000044 DAWFLT010000000
VERSION     DAWFLT010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15315
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.044"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            1..149
                     /locus_tag="WGR74_001330"
                     /pseudo
     CDS             1..149
                     /locus_tag="WGR74_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            441..1595
                     /gene="obgE"
                     /locus_tag="WGR74_001331"
     CDS             441..1595
                     /gene="obgE"
                     /locus_tag="WGR74_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ0905744.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTG
                     ENVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            1678..2727
                     /locus_tag="WGR74_001332"
     CDS             1678..2727
                     /locus_tag="WGR74_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0905745.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3415..4836
                     /locus_tag="WGR74_001333"
     CDS             3415..4836
                     /locus_tag="WGR74_001333"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ0905746.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPPADPMPSEAGDDYTRRFYVAMNDDFDTVKAVAVLFELAGEVNKTNNAQL
                     AGRLKALGGIIGLLQRDPTEFLQGGVASDGLSNEEIEDLIARRKQARSDKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4919..5698
                     /gene="xth"
                     /locus_tag="WGR74_001334"
     CDS             4919..5698
                     /gene="xth"
                     /locus_tag="WGR74_001334"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0905747.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIATSGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE"
     gene            5685..6029
                     /locus_tag="WGR74_001335"
     CDS             5685..6029
                     /locus_tag="WGR74_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905748.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPTWALVWLLICLPCLLIASKCLGWKGWRLLANVLACLAVCALLSVPAA
                     LLIAFTLQDLLK"
     gene            6039..6569
                     /locus_tag="WGR74_001336"
     CDS             6039..6569
                     /locus_tag="WGR74_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0905749.1"
                     /translation="MESFELEDLTLWLVRDADEAEMWIDRWAISYPVVQMSEASAGQS
                     IGEWRAGLQTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVPEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6662..7777)
                     /gene="asd"
                     /locus_tag="WGR74_001337"
     CDS             complement(6662..7777)
                     /gene="asd"
                     /locus_tag="WGR74_001337"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ0905750.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQPLRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAIHAKVADELADPSSAILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRIGSMRCHS
                     QALTLKLKKDLPVSEIEAILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLEMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
     gene            7953..8534
                     /locus_tag="WGR74_001338"
     CDS             7953..8534
                     /locus_tag="WGR74_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0905751.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(8596..9450)
                     /gene="folD"
                     /locus_tag="WGR74_001339"
     CDS             complement(8596..9450)
                     /gene="folD"
                     /locus_tag="WGR74_001339"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ0905752.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            9673..9750
                     /locus_tag="WGR74_001340"
     tRNA            9673..9750
                     /locus_tag="WGR74_001340"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9708..9710,aa:Pro,seq:tgg)
     gene            9775..9851
                     /locus_tag="WGR74_001341"
     tRNA            9775..9851
                     /locus_tag="WGR74_001341"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9809..9811,aa:Arg,seq:tct)
     gene            9879..9954
                     /locus_tag="WGR74_001342"
     tRNA            9879..9954
                     /locus_tag="WGR74_001342"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9912..9914,aa:His,seq:gtg)
     gene            10089..10595
                     /locus_tag="WGR74_001343"
     CDS             10089..10595
                     /locus_tag="WGR74_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HEZ0905753.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGGALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            10592..12370
                     /locus_tag="WGR74_001344"
     CDS             10592..12370
                     /locus_tag="WGR74_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ0905754.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLKTQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDEQGVLHLETAEGKQTVVSGEISLRPDNRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            12483..13307
                     /locus_tag="WGR74_001345"
     CDS             12483..13307
                     /locus_tag="WGR74_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ0905755.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(14070..14858)
                     /locus_tag="WGR74_001346"
     CDS             complement(14070..14858)
                     /locus_tag="WGR74_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ0905756.1"
                     /translation="MLTLYSETFPSRLLLGTAAYPTPEILKQSIQTAQPAMITVSLRR
                     AGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAEPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(15072..15266)
                     /gene="thiS"
                     /locus_tag="WGR74_001347"
     CDS             complement(15072..15266)
                     /gene="thiS"
                     /locus_tag="WGR74_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ0905757.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQKPFAVAVNTVFVPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT         3694 a         3864 c         4216 g         3541 t
ORIGIN      
        1 caagcaaaac gtcatttacg caagtttaac ggcattccca aagcgcattt tgagctgtat
       61 ttaaaggagt gcgaatggcg ttttaacaac agtgagataa aagttcttgt tccattttaa
      121 aacaattagt aaaaccgagt ttatcctagt tatctaggac agccccaaaa cttattatat
      181 gtaacggttt gaacggacat tccgtcgggt cggaatgtca aaaaggcgcg attgtaccaa
      241 agaaggtgga cataattttg tttgcaggct gaagatttgc ttaaaaattc attaagatgg
      301 gcggaacaaa tagtttgggt acggcgtgtt gaaataccgc ttatccctta taatagcgtc
      361 cgaaattctg ttcggacggc atcaaaacac acgccatgcc gtctgaaccc ccatttgaaa
      421 ccaacaaaca agagcattga atgaaattca tcgacgaagc aaaaatcgaa gtcgccgcag
      481 gcaaaggcgg taatggcgca accagtttcc gccgcgaaaa attcgtaccg cgcggcggcc
      541 cggacggcgg cgacggcggc aaaggcggca gcgtttgggc agaagccgac gaaaacacca
      601 acaccctcgt cgaataccgc ttcgtcaaac gctaccaagc caaaaacggc gaaaaaggcc
      661 acggttccga ccgttacggc gcaggtgcgg acgacatcgt cctcaaaatg cccgtcggca
      721 cccttatccg cgacctcgac accggcgaaa acgttgccga cctcacttat cacggacagc
      781 gcgtctgcct tgccaaaggc ggcaaaggcg gcttgggcaa catccacttc aaatcgtccg
      841 tcaaccgcgc cccgaaacaa tccacccccg gcgaagaagg cgaagcccgt tccctgcaac
      901 tcgaactcaa agtcctcgcc gatgtcggct tattgggtat gcccaacgcc ggcaaatcca
      961 ccctgattac cgccgtatcc gccgcacgcc ccaaaatcgc caactacccc ttcaccaccc
     1021 tgcatccaaa cttaggcgtg gtacgcatcg acgaaaacca cagcttcgtg atggccgaca
     1081 tccccggcct gattgaaggc gcggcagaag gcgcaggcct cggccatcgt ttcctcaaac
     1141 acttatcacg taccggcctg ctgctgcacg tcgtcgattt ggcgcccttc gacgaaaccg
     1201 tcaaccctgc cgaagaagca cttgctatca tcaacgaatt gcgcaaatac gatgaagaac
     1261 tttacggcaa accgcgctgg ctggtgctga ataaactcga catgcttgac gaagaagaag
     1321 cccaaacgcg cactgccgcc ttcctcgaag ccgtcggctg ggactacccc aaacccgacg
     1381 accgtttcca atttgacatg gaaaccccgc gcctcttcca aatcagcgcg ctgacccacc
     1441 aaggcacgca ggaattggta caccaaatca accaatacct gaccgagaaa aaacgtatcg
     1501 aagctgaaaa agccgaagcg gagaaagcag cggcaaatgt tgaaattatc gaacagcagc
     1561 ctaaaacgga tacgggcgtg tttaagccgg agtgaaaaaa gaaagggttt cagacgatat
     1621 ttgttctaca aaaggttgtt tgaaacaatt ttaaagcaag attgaaatgg attaaaaatg
     1681 tcatctaacc aatatatcca gtcgcttgag ttgacaaact ttaccgttct tccaaacgat
     1741 acttttgaat tttctgaaaa tctcaatgtt attgttgccg aaaacggttg tggcaaaaca
     1801 catttgttaa aaattttata cagcctgcta gaagtaacat ctaacaccaa aaacagatta
     1861 ctgaaaacag aattgcaaaa aagttttgcc gataaattac tgaatgtttt ccgtcccgat
     1921 tccttgggca ggttgagcaa acgtttgcag gggcgcgggc gtacggaaat cgttttaaaa
     1981 ctgcaaaacg gcactacgca tagtagtctg aatttttcca gtaactcatc atcgcaagtc
     2041 aatgttcaaa gcatcggatt gaaagaaaat gaatatactc cgacacctat cttcttaccc
     2101 agccgcgaat taattacact ttgcccttgg ttcacttctt tgtatcaaaa ccaaagcatc
     2161 cctttcgaag aaacttggtt tgatacctgt atgcaattaa atcacccgct tgccaaaggc
     2221 cccagagaaa caaaaattcg agaattgctc gaacctattg agaacgcgat gggaggaaaa
     2281 gtatctgaag aacaaggaag gttttatcta tcgctgacaa atacgggcgg taagattgaa
     2341 gcacctttgg tagcagaggg tttgcggaaa tttgtcatga ttgcccggtt aatcgcaaca
     2401 ggggcattgc tggataaagg ctatctattt tgggatgaac cggaagctaa tctgaatcct
     2461 aaattgatca aagtggctgc ccgtattatt tggtctttat ctcaacaggg tattcaggtt
     2521 tttattgcaa cacactctct attcttattg agagaattgg aacttttgaa gcttgaaaat
     2581 aacacggatt tacctgtacg ttttttctcg ttgattgctt ccgatgatgg aacaaaagtg
     2641 gagcaaggga acagtattaa tgatttgaac acattgatat tgttagatga aaacattatg
     2701 caggcagaca ggtatctgaa tatttgaggt taagctatga ctacaatcac agaaaagaga
     2761 ttaacctttg cttttcctga agattattac gttaccaagt atgacgaatg ggaacattat
     2821 aaaatttttc agaacagttg taatttacgt aataaaattg atacaaatga aaagggaaag
     2881 aacgggataa atcaaagtgt agatgacgat aatggcagct caggtgtaga tataatagcc
     2941 cttcatgagt cgacactttg gctaatcgaa attaaagatt attaccgact tgggcttgag
     3001 ccaaatgccc aatcaataga tgaaaaactt tctgacctac catatttaat cgcaagaaaa
     3061 attcgagatt cattggcagg attggtttct gctaaattta aagcagaaaa acaggaagaa
     3121 aaagattttt ctcgcttggc tttagattgt aacgaaataa aaatagtcct tcatattgaa
     3181 atgcctagta taagaagtaa actttatccc tcgtcatcag atttagcaaa cttattaaaa
     3241 gataagttta aattatcaga atttacaaaa aattttgcaa actgttatgc agaaccaatt
     3301 ttcacaaata tcagccatat taataatccg caactacgta atgttccttg gtccgtatct
     3361 acaggaacag aacagaaatt atcatccgag caacaaagat taatccataa tcccatgacc
     3421 accatctaca acacccttac ccgccaaaaa gaaccctttg cccccatcga ccctgaaaac
     3481 gtgcgtatgt acgtttgcgg catgactgtt tacgattatt gccacttagg ccatgcccgt
     3541 gtgatggttg tgttcgacat gattgcccgt tggttgcgtg agtgcggcta tccgctcact
     3601 tatgtacgca acatcaccga catcgacgac aaaatcattg cccgtgcggc tgaaaacggc
     3661 gagaccatcg gcgagctgac tgcgcgtttc attcaggcta tgcacgaaga tgccgatgct
     3721 ttgggtgtgt tgcgtcccga tatcgagccg aaggcgacgg aaaacattcc gcaaatgatt
     3781 gccatgattg aaaccctgat tcaaaacggc aaggcatatc ctgccgcaaa tggcgacgtt
     3841 tactacgccg tgcgtgagtt tgccgcttac ggacaactgt cgggcaaatc gttggacgac
     3901 ctgcgcgcgg gcgaacgcgt ggaagtggac ggtttcaaac gcgatccgct tgattttgta
     3961 ttgtggaaag cggcgaaagc aggcgaaccg gcgtgggaaa gcccgtgggg caacggccgt
     4021 ccgggttggc acattgaatg ctctgccatg agtgaaaacc tgttcggcga taccttcgac
     4081 atccacggcg gcggcgcgga tttgcaattc ccgcatcatg aaaacgaaat cgcccaaagc
     4141 gtcggtgcca gcggtcatac ctgcggccac gaccacgcac aaacccacca cggtcaaagc
     4201 attgccagcc acgtcaaata ttggctgcac aacggcttta tccgcgtgga cggcgaaaaa
     4261 atgtccaaat cactgggcaa cttcttcacc atccgcgaag tgttgaaaca atacgacccc
     4321 gaagtcgtgc gcttcttcat cctgcgcgcc cactaccgca gcccgttgaa ctattccgac
     4381 gcgcatttgg acgacgcaaa aggcgcgttg acgcgtttgt atacgacttt gaaaaatacg
     4441 ccgcccgccg atccgatgcc gtctgaagcc ggcgatgact acacgcgccg cttttatgtt
     4501 gcgatgaatg atgattttga tacggtgaaa gcggtggcgg tgttgttcga acttgccggc
     4561 gaagtgaaca aaaccaataa cgcgcaactg gcaggccgtc tgaaggcttt gggcggcatc
     4621 atcggactgc tgcaacgcga tccgaccgag ttcctgcaag gcggcgtggc ttcagacggt
     4681 ctctccaacg aagaaatcga agacttgatc gcccgccgca aacaggcgcg ctccgataaa
     4741 aactgggcgg aatccgaccg catccgcgac cttctgaacg aacacaaaat cattttggaa
     4801 gacaacgccg gcggcacgac ttggcggcgc ggttaaaatc catcgaaaag ggtaaatgtg
     4861 ccgcattttg cccttttttg ttcagacggc atttttattg tgagaactta tttttattat
     4921 gcttaaaatc atttccgcca acgtcaacgg catccgctcc gcctacaaaa aaggttttta
     4981 cgaatacatc gccacatcgg gcgcggacat tgtctgcgtg caggaactca aagcgcagga
     5041 ggctgatttg tctgccgata tgaaaaatcc gcacgggatg cacggccatt ggcattgcgc
     5101 cgagaagcgc ggttacagcg gcgtggcggt gtacagcaaa cgcaaacccg acaatgtgca
     5161 aatcggtatg ggcattgaag aattcgacag ggaagggcgg tttgtgcgtt gcgatttcga
     5221 caggttgagc gttatttcgc tttacttgcc cagcggcagc agcgcggaag aacgccagca
     5281 ggtgaaatac cgtttccttg atgcgtttta ccctatgctc gaagcgatga aaaacgaagg
     5341 acgcgacatt gtcgtttgcg gcgactggaa catcgcccac caaaacatcg acctgaaaaa
     5401 ctggaaaggc aatcagaaaa attcgggctt cctaccggaa gaacgcgagt ggataggcaa
     5461 ggttatccac aagctcggtt ggacggatat gtggcgcacg ctttatcccg atgtgccggg
     5521 ctacacgtgg tggagcaacc gcgggcaggc gtatgcgaaa gatgtcgggt ggcgcatcga
     5581 ttatcagatg gttacgcccg aacttgccgc caaagccgtg tccgcacacg tttataaaga
     5641 tgaaaaattt tccgaccatg cgccgctggt catggagtat gactatgctg ccgaataaag
     5701 ttttgggtaa atatgattgg aatgtggacg gaaaaacagg aataggcgca gcatgggttg
     5761 tcgctgcgtt tatcctgccc atattggtgt gggcgatatt tatgttgtcg cggatgcaag
     5821 gctggcttgc gccgaccaag gcaaacccga catgggcgtt ggtgtggctg ctgatctgcc
     5881 tgccctgcct gctgattgcg tccaaatgtt tgggttggaa aggctggcgg ctgttggcaa
     5941 acgttcttgc ctgcctcgcc gtgtgcgctc tgttgagtgt gcctgccgcg ctgctgattg
     6001 cgttcacgct gcaagacctg ctcaaatagg cgcggacaat ggaaagtttc gagcttgagg
     6061 atttgacgct gtggctggta cgcgatgcgg atgaagcgga aatgtggata gaccgctggg
     6121 cgatcagtta tcccgtcgtg cagatgtccg aagcgtcggc cggtcaaagc ataggggaat
     6181 ggcgggcagg gcttcagacg gcatttgaac gcatacgcgg caaatacgtc gccgttgtcg
     6241 cacacggtgc gggcgcggcc gcatttttgg cgtggctgta tcaggcagac atcctgacac
     6301 ggaagaaaat tgccaacatc atccttgtac cgcagcgtcc cgatattttt cccgacgatg
     6361 cggaacacac tttccaacgc gtccgctgtc cctgccgtgc cgcattggtt gtacccgaac
     6421 acggcggcgt gccgcacggt tgggcgcaaa aacaggcgga tttgtggaac gcccgcctgt
     6481 tggtttcccc gcattcgggc agtttgaacg gcatgctcgg cggctggcag tggggtatga
     6541 agctgatgca ggaaatgttg ctggcgtgaa cgcttgcctt atcggaattt cggttaactg
     6601 gggttgcaag gaaaatgccg tctgaaacag gcttcagacg gcatttccat tcaaacaggg
     6661 cttacaggct gcccaacacg atacgcagca cgcggcgcaa cggctcggca gccccccaca
     6721 agagttggtc gccgacagtg aacgcgctga tgtattcgcc gcccatctcc agtttgcgga
     6781 tacggccgac aggaacagac agtgtgccgg taactttggc aggagtcagc tcgtgtatgc
     6841 tggcttcttt ttcgttaggg atgactttca cccagtcgtt tgcacctgcc aaaatcgctt
     6901 cgatttcgga aacaggcagg tcttttttca gcttcagggt gagggcttgg ctgtggcagc
     6961 gcatagagcc gatgcgtacg cataagccgt cgattacggt cggattgtcg ctgcggccga
     7021 ggattttgtt ggtttccaca ccgcctttcc attcttcttt agattgaccg ttgcccaaat
     7081 ccacgtcaat ccacggaatc aggctgccgg cgagcggtac gccgaagttg gctttcggat
     7141 agtcttcgct gcgcaggaaa tcggacactt tgcggtcgat gtcgagaatc gcgctggaag
     7201 gatcggcaag ctcgtccgct actttggcgt gaatcgcgcc catgccgctg atgagttcgc
     7261 gcatgttttt cgcacccgca ccggaagcgg cttggtaggt catgctggtt gcccattcga
     7321 ccaaatcgtt ttggaacaga ccgcccaaag ccatcagcat cagggaaacg gtacagttgc
     7381 cgccgatgta gtttttcacg ccgtttttga ggccgttgtc gatgacgttg cggttgaccg
     7441 ggtcgaggac gataatcgca tcgtctttca tgcgcaggga tgacgccgcg tcaacccagt
     7501 agccgttcca gccgctgtcg cgcaggggtt ggaagacgga tttggtgtaa tcgccgccct
     7561 gacaggtaac gatgatgtcc atttttgcca attcggcaac atcgttggca tccaacaatg
     7621 ttttggctgc ctgaccgaaa tcaggggctg cgccgccgac gttggaagtg gtaaagaaaa
     7681 acgcttcagg aatgtgggcg aagtcgtttt cttctttcat acgctgcatc aaaaccgaac
     7741 cgaccatacc gcgccagccg acgaaaccta ctttcattgt gttgctcctt gaggaaaaat
     7801 cgttaataaa attatgtatg gcgtaaagga agcgttttaa cgccgtattg ggaaaatgta
     7861 aagttcttta tcgggctttc tttaagtatt ggcgttttcg gtgtcgtggc ggtagaatcg
     7921 gggtcagtta agaacaatta tcaggaacgg atatggaaga aaaaaacgac tacaccgacg
     7981 cggcatccga caaccgcaac gggcaggaaa ttgaagtcgg aattgccggc gcaggcgacc
     8041 ggatacttgc cgcgctgctg aaccaattgt ttaccttttt ggttttgttg gtgccgtttg
     8101 ccggactgat tgctttcgcc atcaagaatg aaggcaggat tggcggcagt gaggaaatat
     8161 tcgggctgct tttgggcatg acttcctttt gggtaggact ggcgggcatt ttggtttata
     8221 ccgtcgttca aatctactat atgagtcggg acgggcagtc attgggtaag aagatcatga
     8281 gaatcagggt gttgaaaacc gacggccgca atccgggttt tgtcggcacg gttttggtac
     8341 gcgaaatcgc atggtcggtt ttggttgcca ttattgccgc cgttatcggt cttgcagtag
     8401 gtgacaacgg agaaaacgcc atcaacctgc tggcattcct tgccaacttt gtcctgctct
     8461 ttatggtcaa acgcgaccgc cgcacgcttt acgacatact ggcggatacg gttgtcgtca
     8521 agctgcccaa ataaacggac acggcaaaat gccgtctgaa agcctttcag acggtatttt
     8581 tatcttcaga accgctcaag catcgtgcag tgaagccgcg tgcagggtgt tttccatcaa
     8641 tgtggcaatc gtcatcggac ccacgccgcc gggaacgggc gtaatcatcg ccgcccgttc
     8701 ttttgccgtt tcaaattcca cgtcgccgca caggctgcca tcgtccaaac ggttgatgcc
     8761 cacatcaata acgaccgcgc caggtttgat ccattcgcct ttgacaaagt tcggaatgcc
     8821 tacgccgacc accaaaatat cggctccggc aacctcgtct gtcagatttt cggttgcgct
     8881 gtggcagacc gttaccgttg cgcgcgccag cagcagttcc aaagcctgcg ggcggccgac
     8941 gatattcgac gcgccgacca cgaccgcttt tttccccttc ggatcaatgc cgtaagcttc
     9001 caaaagcgtc atcacgccct tgggcgtaca cgggcgcatc agcggcattt tgaccgccag
     9061 cctgccgaca ttgtaaggat ggaagccgtc cacgtcctta tccggcgaaa tacgttccaa
     9121 aaccgcctgg ctgtcgaggt gcttcggcag cggtagctga accagaatac cgtccacttc
     9181 ggaatcggca ttcaggcggt cgaccagtgc cagcagttct tcctgcgatg ttgattcggg
     9241 cagctcgtaa gacagtgatt tgatgccgca tttttggcag gcggttttct tgttgcggac
     9301 ataaaccgcg ctggcagggt cgcctccgac caaaaccacg gccaggcaag gcatgtgcag
     9361 attgtcctgt tggcgttgcg ccaccgcttc ggcaaccgcc tgcaggcgtt tttgcgaaac
     9421 ttctttacca ttgatcagtt gtgccgacat atccgtcctt ttgttaaaca ggtagaaaat
     9481 gctgaaaatt atcgcatttt ccgtttgttt ttacagcagg atgccgaaaa taaatttgcc
     9541 ctgaaataaa atacggctgt ttggacggat taaatatttt ttcaaatcaa aaaactatat
     9601 ttttcatctt ggtgtcttga aaataaatat tgacgcatct ttccctgagt tgtatagttc
     9661 gcttcttcaa gtcggggcgt agcgcagccc ggttagcgca tctgctttgg gagcagaggg
     9721 tcgtgagttc gaatcccacc gccccgacca atcaatcagc acgaatcggc atgagcgccc
     9781 gtagctcaac cggatagagc accgaccttc taagtcgggg gttacaggtt cgattcctgt
     9841 cgggcgtgcc aagaatttga ttgaaaaccg tttttatggt ggctgtagct cagttggtag
     9901 agccccggat tgtgattccg gttgtcgtgg gttcgagccc catcagccac cccagataaa
     9961 ggcccgtact tttcggtacg ggtcttttct cgtttgcgcg tactgtccgc gccgccggct
    10021 ttgcctttcg catatgcttc tgtatcatgt aggttttctg tcttttatgg tttttgtcgg
    10081 agactttgat ggttgacgct tggtctgttc ctgattttga atcgaaaatc tgtccgcccg
    10141 aggcgttggc ggcgcgtttg gcgttgttgc cgcgcccgct ggtgtttacc aacggctgtt
    10201 tcgacatcct ccacaggggg cacgttactt atctggcgca ggcgcgttcg atggggggcg
    10261 cgttggtgct ggcgttgaat accgatgctt cggtgcggcg tttgggcaag ggcggcgacc
    10321 gcccgattaa tcctttggag aaccgtgccg ccgttgccgc cgcgttggaa agtgtggatt
    10381 tggtaacgtg gtttgacggg gatacgccgg cggcgttgat tgaggcggtc aaacctgaga
    10441 ttttggtcaa gggcggcgat tgggctgcgg ataagattgt cggtgcggca gaaacgttgg
    10501 cgcgcggcgg tcaggtgttt tcaattccgt ttctgcacca gacttcgaca acgaagactt
    10561 tggcaaaaat ccgtgcggca gagggcggaa aatgacggtt ttgaagcctt cgcactggcg
    10621 ggtgttggcg gagcttgccg acggtttgcc gcaacacgtc tcgcaactgg cgcgtatggc
    10681 ggatatgaag ccgcagcagc tcaacggttt ttggcagcag atgccggcgc acatacgcgg
    10741 gctgttgcgc caacacgacg gctattggcg gctggtgcgc ccattggcgg ttttcgatgc
    10801 cgaaggtttg cgcgagctgg gggaaaggtc gggttttcag acggcattga agcacgagtg
    10861 cgcgtccagc aacgacgaga tactggaatt ggcgcggatt gcgccggaca aggcgcacaa
    10921 aaccatatgt gtgacccacc tgcaaagtaa gggcaggggg cggcaggggc ggaagtggtc
    10981 gcaccgtttg ggcgagtgtc tgatgttcag ttttggctgg gtgtttgacc ggccgcagta
    11041 tgagttgggt tcgctgtcgc ctgttgcggc agtggcgtgc cggcgcgcct tgtcgcgttt
    11101 gggtttgaaa acgcaaatca agtggccaaa cgatttggtc gtcggacgcg acaaattggg
    11161 cggcattctg attgaaacgg tcaggacggg cggcaaaacg gttgccgtgg tcggtatcgg
    11221 catcaatttc gtgctgccca aggaagtgga aaacgccgct tccgtgcaat cgctgtttca
    11281 gacggcatcg cggcggggaa atgccgatgc cgccgtgttg ctggaaacgc tgttggcgga
    11341 acttgatgcg gtgttgttgc aatatgcgcg ggacggattt gcgccttttg tggcggaata
    11401 tcaggctgcc aaccgcgacc acggcaaggc ggtattgctg ttgcgcgacg gcgaaaccgt
    11461 gttcgaaggc acggttaaag gcgtggacga acaaggcgtt ctgcacttgg aaacggcaga
    11521 gggcaaacag acggtcgtca gcggcgaaat cagcctgcgg cccgacaaca ggccggtttc
    11581 cgtgccgaag cggcgggatt cggaacgttt tctgctgttg gacggcggca acagccggct
    11641 caagtgggcg tgggtggaaa acggcacgtt cgcaaccgtc ggtagcgcgc cgtaccgcga
    11701 tttgtcgcct ttgggcgcgg agtgggcgga aaaggcggat ggaaatgtcc gcatcgtcgg
    11761 ttgcgccgtg tgcggagaat tcaaaaaggc acaagtgcag gaacagctcg cccgaaaaat
    11821 cgagtggctg ccgtcttccg cacaggcttt gggcatacgc aaccactacc gccaccccga
    11881 agaacacggt tccgaccgct ggttcaacgc cttgggcagc cgccgcttca gccgcaacgc
    11941 ctgcgtcgtc gtcagttgcg gcacggcggt aacggttgac gcgctcaccg atgacggaca
    12001 ttatctcggg ggaaccatca tgcccggttt ccacctgatg aaagaatcgc tcgccgtccg
    12061 aaccgccaac ctcaaccggc acgccggtaa gcgttatcct ttcccgacca caacgggcaa
    12121 tgccgtcgcc agcggcatga tggatgcggt ttgcggctcg gttatgatga tgcacgggcg
    12181 tttgaaagaa aaaaccgggg cgggcaagcc tgtcgatgtc atcattaccg gcggcggcgc
    12241 ggcaaaagtt gccgaagccc tgccgcctgc atttttggcg gaaaataccg tgcgcgtggc
    12301 ggacaacctc gtcattcacg ggctgctgaa cctgattgcc gccgaaggcg gggaatcgga
    12361 acatacttaa aaaatacacc ctttttcaac acggggcggt atccgtccga atccgtccgg
    12421 attctgccgc ccgacagact gccgcccagg cgggcggggt ttcaaacatt gaggaaattc
    12481 taatgaaatg gctcttcacc cttttggcgg tactcaatat tgccgtattc ggcggcacgg
    12541 tgggctataa attggccgtt aaagcgacgg gcggcgtgcc ggaaaaccgg gccgtcgaaa
    12601 acacgccccc cgcaacgccc gccgccgaca atgcggcggc atccgtggag gatacggcgg
    12661 cactgctcaa accgggcgac atcctgaccg aagagcaggc ggagcagttg cgcttgaaga
    12721 aagaagcgga acagaaaaaa ctgagggaga aaaaacagcg tgaagaaaaa gcccgccgcg
    12781 aaaaactcgc cgccgaaaag gcgcaggcgg aacgcgaaaa cggcgcggcg gatgccttat
    12841 gcgccgcgca ggcaagcctc acgatggacg aagatgacta ccaccgcatc aaaggacttt
    12901 tgggcaaatg gtcgcacgtt gccagcagga gcgtcgaaaa acgcaccgcc aaagccaaac
    12961 ctgccgacaa aacctaccgc gtcgtcctgc ccgtttccgc cgatgccgaa aatcaggcgg
    13021 cggagctgtc tgccaaaggt ttcaacccca taccgtttga cggcgcattg agtttgggtg
    13081 tcggcaacag ccgggaaaac gcccaagccc tgcaaaaccg gcttgccggc gccggattcg
    13141 gcggggcgca tattgtcgaa cactttgccg aagccgacag gcaggacgat tctttgagcg
    13201 tgtcgcgtat gacggttttg tttaccggcg tgaatgccgc cgatgcggac gaaatccgta
    13261 aaatcacgtc cctatacggc aaactgaacc tcaagtcttg caaataggcg cgaaagccgg
    13321 acgataaacg ccaaacccac cgcgaaggtg ggtttggcgt ttcggttttg agtatctgat
    13381 atataaaaag ggaatgtatg tctcgtatat gccgactcca agtgtgaaag tgatgatggg
    13441 gaaatatggt gtttaatggt tcggaacgga cggcttccga gtggttttga ttatacgcta
    13501 ataataataa ttatcaataa aaatttaaaa ataaatgcag attttttgta atacgatgaa
    13561 aaataaacaa attaacctgt ccgccgatgt ttgtgattcg ggtcggcaaa ctttcattgt
    13621 gtcggacaaa tcggggtcgg cagtcatagc tgagggcttc gtgcctgacg gaaataaggt
    13681 tgcccgtgtt tgaacatccg tccgattggg cgcggacagg gttgtcattc gggggtgttt
    13741 gagcgtggat tcggggatgc gaaagcggta aaaccgttgc ggatgaaggc ttacggacgg
    13801 ctttaggtat ttttaagtgt attcaaacaa caacaaaacg cttcttctat tgccgtattt
    13861 agggcttgcg cggcagcctg cacaccgtgg aggccgagtg tttcgttggt tgcggcatcg
    13921 ggacgggcgg caaggttttc cggccggttt ttccattttt tccgacaaag ccgtgatgcc
    13981 ggggaatggc ggtttgtccg tttcggaccg ccgccgtttt gagccgcgat gccgtctgaa
    14041 ccgttcagac ggcatttttg ccgccttttt caatattccg ccgaatgcca aaacggctgt
    14101 ccgactgtcg gcgtgctggc ttgcgctttg tcgcgtgctt cgaccggttc ggcttcaaat
    14161 gccagccgtc cggattcgac ggcgagtgcg aaggcgcgtg ccatattgac cggatcgccg
    14221 ctgcgggaaa cggcagtatt caaaagcacg ccgtcaaaac cccattccat cacttgtgcc
    14281 gcctgtgagg gcaaacccaa gcccgcgtcg ataatcagcg gcgtgtcggg caggcgttcg
    14341 cgcaggacgt tcaacgcgta ggcgtgaacc gcgcccaaac ccgtgccgat cggagccgcc
    14401 cacggcatta acgcctgaca gcccgcgtcg agcaggcggc ggcaggcaat caggtcttcg
    14461 gtgcaataag gcagcacttt gaagccgtct ttaatcagga tttccgccgc ttcgacaagt
    14521 tggaacacat ccggctgcaa ggtgtcgtca tctccgatga gttccaattt tatccaatcg
    14581 gtttcaaaca cttcgcgcgc catttgcgcc gtcgttaccg cttcctgcac gctttggcag
    14641 cctgccgtgt tcggcaggac gggaacgccg gtttcttgaa gcagcgacca aaacccctga
    14701 ccgtgcgcct cgccgccgct tcccgcgcgg cgcagcgaga cggtaatcat cgcaggctgg
    14761 gcggtttgga tggattgttt gaggatttcg ggggtcgggt aggcggctgt gccgagcagc
    14821 agccgcgaag ggaaagtttc gctgtacagg gtgagcataa tgggttcctt tgtatagtgg
    14881 attaacaaaa accagtacgg cgttgcctcg tcttagctca aagaaaacga ttctctaagg
    14941 tgctgaagca ctaagtgaat cggttccgta ctatttgtac tgtctgcggc ttcgtcgcct
    15001 tgtcctgatt taaagttaat ccaccataac gttgttttgg ggacaggggt cgtctgaaaa
    15061 ggcaaaaccg cctagccgcc gaccaccggc cgcacgatat cgattttgtc gttttcgttt
    15121 aaaaccgttt ccgcatacgc gcctttgggg acgaaaacgg tgttgaccgc cacggcaaag
    15181 ggcttttgcg gcgcggtttg ggcgatgagg tcggcaacgc ttgtgccgtg aagttcggcg
    15241 ggtccgccgt ttaagatgat gttcatggtt tctcgatgtt tctgtattta tagtggatta
    15301 acaaaaacca gtacg
//
LOCUS       DAWFLT010000045        15263 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWFLT010000045 DAWFLT010000000
VERSION     DAWFLT010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15263
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.045"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(79..315)
                     /locus_tag="WGR74_001348"
                     /pseudo
     CDS             complement(79..315)
                     /locus_tag="WGR74_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
     gene            509..967
                     /locus_tag="WGR74_001349"
                     /pseudo
     CDS             509..967
                     /locus_tag="WGR74_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            867..1235
                     /locus_tag="WGR74_001350"
     CDS             867..1235
                     /locus_tag="WGR74_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ0905758.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            1304..2854
                     /gene="lnt"
                     /locus_tag="WGR74_001351"
     CDS             1304..2854
                     /gene="lnt"
                     /locus_tag="WGR74_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ0905759.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTTRSGKRLLPIILIAALIAALLAAGYT
                     ARQTDFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPET
                     AIPVMRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQD
                     GIPYYAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNI
                     CYEDGFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATN
                     TGATAIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALIL
                     FIFRNKEH"
     gene            3118..3981
                     /gene="rpoH"
                     /locus_tag="WGR74_001352"
     CDS             3118..3981
                     /gene="rpoH"
                     /locus_tag="WGR74_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ0905760.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            4465..5349
                     /locus_tag="WGR74_001353"
     CDS             4465..5349
                     /locus_tag="WGR74_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ0905761.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDLRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDTENSRQSLELNSDLVNQLAEINKDLR
                     KHHDLAKLGVADILRFPGVLASQRENTEELAKSITELTEKALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIDALSELFPTLLETHKEKIRARLVEAVGDINNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(5433..5948)
                     /locus_tag="WGR74_001354"
     CDS             complement(5433..5948)
                     /locus_tag="WGR74_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0905762.1"
                     /translation="MMTLKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(6024..6443)
                     /locus_tag="WGR74_001355"
     CDS             complement(6024..6443)
                     /locus_tag="WGR74_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905763.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(6564..7562)
                     /locus_tag="WGR74_001356"
     CDS             complement(6564..7562)
                     /locus_tag="WGR74_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ0905764.1"
                     /translation="MAAYIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNTFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHTVNIDEAQTAVANVARFDVFQLAGA
                     WMKGDVPRVCRLLDGLQEEGEEPVLLLWAVAEDIRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAKIDRIIKGAEEGDAWAAFRQLVISLAE"
     gene            complement(7564..8043)
                     /locus_tag="WGR74_001357"
     CDS             complement(7564..8043)
                     /locus_tag="WGR74_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905765.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQALGRVDDAAGAQMTLRIDSVSQNKETYTITRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEETLWAEMRQDAAEQIVRRLTFLKAE"
     gene            complement(8095..8874)
                     /gene="pgeF"
                     /locus_tag="WGR74_001358"
     CDS             complement(8095..8874)
                     /gene="pgeF"
                     /locus_tag="WGR74_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ0905766.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPTNVKTLITTRNGGVSQGAY
                     QSLNLGSHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTIVVNAAETLGGTPDADAS
                     VDDTSKAVCAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEI
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAATAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            9481..10428
                     /locus_tag="WGR74_001359"
     CDS             9481..10428
                     /locus_tag="WGR74_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ0905767.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFEHPKVVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAG
                     SMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLTIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(10581..11705)
                     /gene="rluD"
                     /locus_tag="WGR74_001360"
     CDS             complement(10581..11705)
                     /gene="rluD"
                     /locus_tag="WGR74_001360"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ0905768.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQIPRAGIVHRLD
                     KETSGLMVVAKTLSAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLAHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPKTGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            11704..12507
                     /locus_tag="WGR74_001361"
     CDS             11704..12507
                     /locus_tag="WGR74_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ0905769.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(12565..14784)
                     /locus_tag="WGR74_001362"
     CDS             complement(12565..14784)
                     /locus_tag="WGR74_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ0905770.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWWAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLATFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLVSPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         3688 a         4591 c         3723 g         3261 t
ORIGIN      
        1 cgaccttgcc gtcgggcagg atgtaccaca ccaaatcccc gcctgaagca cggtgtcgga
       61 ctgcatttcg attcggctat acgccttttc acgcaggctg taattgaatt tccacaaacc
      121 gccgaacact gccggcggac gcgcgaccag catcaggaac gccgcaatcg ccaaggcttc
      181 cgccgccccg tccaacacgc cggcgggaga aaccagcagc aaaggcattc ccaaacgtgc
      241 gggtaaagtg gtcgaaacca cgctcagaaa caacagtgcg ccacccggca ggaacagact
      301 gcccatccaa tccacgctaa cgattctccc ttaaaattca ttaaattaaa atcgaaagac
      361 tgtaacacgg catttttttc acgaaacttt acttcacgtc cgccaccgcc ttcagacggc
      421 attttcctgt ttcatgccgc ctgaaaccgc ccaaatccgc gttctcctat ataatgtcgg
      481 actttgcgcc gcatttcagg attctttaat ggcatttgcc tcactattta ccctgccgga
      541 cgacatcacc gccgttttgg acgatgttgc cctgatgacc aaagccgccg caaagaaaac
      601 cgccggcatg gtcggcgacg atttggcacc caatgccaac cgggttacgg gcgtatcggc
      661 agaacgcgag ttgccgattg tttggtcgat ggcgaaaggt tctttttcca acaagccggt
      721 tttggtacct gcggctctgc tgctttccgc cttcctgccg caactcatta cgccgctttt
      781 gacggcaggc ggcatttatc tgtgctttga cggcgtagaa aagctgctgc acaagtttct
      841 gcaccgacat gacgcgcatg ctcgtaatgg cggcaatcgg catcggcatg actgttttgg
      901 tgtacggaat cgttgccgtc atcgtcaaac tcgacgactt aggcatgctg ctgatgcgcc
      961 gcccacaaac cttttcgcgc agtttggggc aaatgctgac tgcctttatg ccttgcttta
     1021 tgcgcggttt gagcgtggtc ggcacgcttg ccatgtttct tatcggcggc gtactggtcg
     1081 cccacaacct cggccttctg cacgatttcc tgcacgcgca acactgggac gctggctggg
     1141 cggagtactt cgccaacctc gttgtcgggc tgctttccgg ttcgattgcc tgcgcccccg
     1201 ccttgccgct gatgaatcgt ttcggcaggc attgatttct ttttcaaata ccgatgccgt
     1261 ttgaaagatg ttcagacggt atcttccgaa cagacagatg aatatggttt ccaaactgga
     1321 caaatactgg cagcaccccg ccctctactg gcctttgctc atcctttttg ccgccgccac
     1381 cccctttacc ttcgcaccct actaccactt ttggctgatg cccttgattt tcggtgcctt
     1441 cgtccgcctc atcgaactgc gtccgcgttt tgctgtctct tccgcctacc tgttcggcct
     1501 gaccgcatac acgacacagt tctactggat acacaccgcc ctgcacgacg tttccggcct
     1561 gcccgacctc tatgccgtac cgctgacctt cctactcccc gcctaccttg ccctttatcc
     1621 ggcactgtgt ttctggctgt ggaaaaaatt taccctgcct cggggcataa aaatcggttt
     1681 ggtactgccc atcctgtgga cgctgaccga gtttgcccgc gaacgtttcc tgaccggatt
     1741 cggctggggc gcaatcggct actcccaaat caccccggac agcccgctcg ccggctttgc
     1801 cccattgggc ggcatccaca tggttacact ggcaaccgcc tttctcggtg tctggctggt
     1861 tttggcgagt gataacacca cacgttcggg caaacgcctg cttccaatta tcctgattgc
     1921 cgccctgatt gccgccctgc ttgccgcagg ctacaccgcc cgacaaaccg acttcacccg
     1981 ccccgacggc agccgcagca ccgtcgccct gcttcaaggc aacatcgacc aaaccctcaa
     2041 atggcgtgaa gaccaagtca tcccgaccat acagaaatat tacgaacagg tcggcaaaac
     2101 cactgccgac atcgtcatcc tgcccgaaac cgccatcccc gttatgcgcc aaaacctgcc
     2161 ggaaaacata ctggcaaaat ttgccgaaca ggcgcaaaac aacggcagcg cgctcgccgt
     2221 cggcatcagc caatacactt cggacggcaa cggttacgaa aacgccgtca tcaacctgac
     2281 cggttatcag gaaaacaatc aggacggcat cccctactac gccaaaaacc acctcgtccc
     2341 cttcggcgaa tacaaaccgc tgcccttcct gaccacgccg ctttacaaaa tgatgaatat
     2401 gcccctttcc gacttccgca aaggcggcgg taagcaatcc gccctgctga tgaaaaacca
     2461 aaaaatcgcc ttcaacatct gttacgaaga cggattcggc gacgaactga ttgccgccgc
     2521 caaagatgcc accctgcttg ccaatgccag caatatggcg tggtacggaa aatccaacgc
     2581 catgtaccag cacctccaac aatcgcaggc acgggctatg gaactcggac gctatatggt
     2641 ccgtgccacc aacaccggcg caaccgccat catctccccc aaaggcaaca tcatcgccca
     2701 agcccaaccc gatacggaaa ccgtattgga aggacacatc aaaggctatg tcggcgaaac
     2761 gccctatatg aaaaccggca gttcatggtg gttgatgggc atattgaccc tagccgcact
     2821 gattcttttc atcttccgaa acaaagaaca ctgatacctg cacagcccgt ccagacgggc
     2881 tgtctttttt caaaccggct gtttacatac aactacgtat gtaatataat tccgaaatcg
     2941 gattgagcca tcaggtttac aaaatcaatc aaacctgcct tacaaaattt tcctgtcttt
     3001 caaaccttag cgcatatctc ctgccaaaca tacaaaatac aagccaagca gtgatatact
     3061 gcatcccaac acagcaaacc gccgatacgc agtttcagcc gaaaggaaat gccccaaatg
     3121 aataacgctt tcgcattacc cgccatccaa agcggcaacg gcagcctcga acaatacatc
     3181 cacaccgtca acagcatccc gatgctgtcc caggaagaag aaacccgcct cgccgaacgc
     3241 cggataaagg gcgacctcaa cgccgccaaa caactcatcc tgtcccacct gcgcgtcgtc
     3301 gtttccatcg cgcgcggcta tgacggctac gggctgaatc aggcagacct gattcaggaa
     3361 ggcaatatcg gactgatgaa ggcggtcaaa cgctacgagc caggtcgagg tgcacgcctg
     3421 ttttcatttg ccgtgcattg gattaaagcg gaaattcacg aattcatcct gaggaactgg
     3481 cgccttgtcc gcgttgccac caccaaaccg caacgcaagc tgtttttcaa tttacgcagt
     3541 atgcgtaaaa acctcaacgc cctgtctccc aaagaggcac aagacatcgc cgacgatttg
     3601 ggtgtcaaac tttccgaagt tctggaaatg gaacagcgca tgacgggaca cgacatcgcc
     3661 atcatggcag acaacagcga cgacgaggac agtttcgccc ccatcgactg gcttgccgac
     3721 cacgattccg aaccgagccg acaactatcg aaacaggcgc attacgccct gcaaacagaa
     3781 ggtttacaaa acgctttggc gcaactggat gacaggagcc gccgtattgt ggaaagccgc
     3841 tggcttcaag acgacggcgg gctgacgctg caccagcttg ccgccgaata cggcgtctct
     3901 gccgaacgca tccgccaaat cgaagcaaaa gccatgcaga aactgcgtgg tttcctgacc
     3961 gaagaagccg aagcggtttg aaatatacgc aataaaatac ccgacaaatt gttttgtcgg
     4021 gtattttatt tggtaaacat caatgcggtt tgtgcgtcgt gccgtacacg attttatccg
     4081 tccacgccgt tgcaattgcc gaaatcaaaa agcagacatg caccagacac tgccgcatca
     4141 gctgcttttc cgacagattg gcggaattga tatagtggat taacaaaaat caggacaagg
     4201 cggcgaagcc gcagacagta caaatcgtac ggaaccgatt cacttggtgc ttcagcacct
     4261 tagagaatcg ttctctttga gctaaggcga ggcaacgccg tactggtttt ggttaatcca
     4321 ctatacattt cccttccttg cagcaacaaa agagcgtgat tccaatctgc cgctgcaaaa
     4381 ccttcaattt ttcacacccg aaaacgcttg ccgccataca gatgtgatat attgattcca
     4441 ttttaacggg cttcggagaa cgacatgagc agcggaaata tccacatcca cagcatgacc
     4501 ggctttgcca acgcggcggc agagtgcggc agcaaacgca tcaacctcga cctgcgtgcg
     4561 gtcaaccacc gttttctaga tattcaaatc aggatgcccg acgatttgcg ctatttggaa
     4621 agcggtatcc gcgagaaaat ttcatcccat atcgcgcgcg gcaaagtcga atgcaaaatc
     4681 caaattcaag atacggaaaa cagcagacag tccttggaac tcaactcgga tttggtgaac
     4741 cagcttgccg aaatcaacaa agacctccgc aagcatcacg atttggcaaa actgggcgtt
     4801 gccgacatcc tgcgtttccc cggcgtattg gcaagccaaa gggaaaatac ggaggaattg
     4861 gcaaaaagca ttaccgaact gaccgaaaaa gcgttaaaag actttactgc cgcacgccgg
     4921 cgcgaaggga aaaaattggg cgagcacctg ttacaacgcc tcgaaagcat ggaagaaatc
     4981 atagatgcat tgagcgaact ctttcctacc ctgctggaaa cacataaaga aaaaatccgc
     5041 gcccgcctcg tcgaagcagt cggcgacatc aataatgaca ggctgcaaca ggaatttgcc
     5101 ctctttattc aaaaatccga catcgacgaa gaattcagcc gcctgcgcac ccacattgcc
     5161 gaagtgcgcc gtatcgttac cgaacacaaa ggcagcagcg gcaaacggct ggacttcctg
     5221 atgcaggaat taaaccgcga agccaacact ttgggcagca aatccattgc cgccgaatgc
     5281 acccaagcct cggtcgaact gaaagtctta atcgagcaga tgcgggaaca agtgcagaat
     5341 atcgaataat cggtttcccc gataaccaaa tgccgtctga aagccctttc agacggcatt
     5401 ttttcaggtt gaaacctttg cggtataaag gcttaaggct ttcctttgac cgccaccaat
     5461 cttgttcctg caagaaaatc atacagaaac tgccgatcgg gattcagcag tgcgaacccc
     5521 cacggtaaaa tcagccaaat caatgccgcg ccggccgcgc ccttgggcgg aatgccgagg
     5581 aagtggcgta atccggcata ggctaacata gggataaata cgataaatat gcacgcccaa
     5641 ataaagcgca ggcgcagcag gtgcaaagac ggctgtatgc cgttaaggtc gcacaagccg
     5701 attttccatg tcctcatcgc caaggtctgc ccctgaccgt gccagttggc gcgaaaataa
     5761 agccaccatg atcccattat caggatactc gttaccaatg cagaaaccgc gatagaaacg
     5821 ggattcagaa aaatggcggc aataccggca aacaatgctg ccaaacaggt tgccgcaccg
     5881 accagcagca tttcgtacag catggcggca aagcggcgtt tgagcggggc ggttttcagt
     5941 gtcatcatct tttcaatcag aatatgccgt ctgaaacgcc aacaggcttc agacggcatt
     6001 tatgcaaaga tgccttgcgg cggttaacgg tgcggacgtt tctgaccgcg cgactgtttt
     6061 gccgccattt gattgatttc ctgacgtgtc gggcgttttt tgaacatagc gtgcgcctcg
     6121 tccaccgaac atttttgcga caggcagatc agggtaatcg catctttctg gtgttgtccg
     6181 ccgctgtggt acatacggcg caggtttgcc tcgttgattc tgcctttttc gggatcgatg
     6241 tcgaattttt tcaggcgttc gataaactgg cgttgctgtc ggcgggatgt ccaaacaaaa
     6301 ccgcccgcag cggcggcgac gatgacggcg gcaagcagga tggcaagcgg ccaggagtcg
     6361 acaatcaata cgataatgag gcttaaaacg gcggtagtca gtaaaatcag gccgcccaag
     6421 cgcagtttgg tcttattgtc catagggtta atgttccttt attgaggtgg tcgtgccgtc
     6481 tgaaatgttt tcggacggat tggtgaatac ttttcaggat taacggtatt ttacagtatt
     6541 ttcggatttt agggattgcc gctttattct gccagtgaaa tgacgagttg tctgaacgcc
     6601 gcccacgcat cgccttcctc cgcgcctttg ataatcctgt cgattttggc acaggttttg
     6661 agcgcgtcaa gcaggcggac gacggaaatc cgcttgaccg caagcggtgc gagcgtctgt
     6721 ttgtcgcccc aaagcctgag gctgttgcgg acggattgga tgctctgccc ctgtttcagg
     6781 gcggcggcaa gccggatcag cgtcctgatg tcttcggcta ccgcccacag caacagtacc
     6841 ggttcttcgc cttcctcctg caatccgtcc aacaacctgc atacgcgcgg cacatcaccc
     6901 ttcatccatg cgcctgccag ttggaacacg tcaaagcggg caacattggc gacggcggtt
     6961 tgcgcctcat cgatattgac ggtatgccct ttcggataca gcagcgcgag cttgtcgatt
     7021 tcctgacgcg ccgccaacag attgccttcc acgcgctcgg caaacagtgc caatgcgtct
     7081 gcctcgatac ccaaaccgat tttgtccagc cgtccgcgta tccattgggg caaggccgcc
     7141 gcgccgaccg gtttggcttc ccacacttcc ccctttgccg ccaatgcggc aaaccatttg
     7201 gactggagct gggttttctc cagtttgggc agtaaaacca gcgttaccgt atcttccggc
     7261 aggcgggcgg caaaatcctg caacgcctcg ccgccggttt tgccgggctt gccgttaggg
     7321 atatgcagtt ccaacagctt caaatcggca aacagacccg cactgcctgc ggtttgcagc
     7381 agttcgttcc aatcgaaggt attgtctgcc gtataaactt cccgattgag gtaaccttgt
     7441 ttcttcgccg ccgccctcaa tgcgtccaat gcctcgatac gcaacagttc ttcctcgccg
     7501 tgaatgacgt acagaggttt caaaggcgcg tccgtgtcga tgcgtccgat atatgccgcc
     7561 acgtcattcc gccttcagaa aggtcaggcg gcggacaatt tgttcggcgg catcctgccg
     7621 catttccgcc cacagggttt cttcttcttc ctgtttgccc aaaatttcgt tgtcggcata
     7681 agcaaggacg cggcggacgg acacggtcat cggtttaccg accggctcgc cgcgtttcaa
     7741 tacctgcgct tcaaccgtca atatcaaaag atattcgttg atgactgccg cacgggtaat
     7801 ggtataggtt tccttgtttt gggaaacgct gtctatacgc agggtcatct gcgcgccggc
     7861 agcatcgtcc accctgccca aagcctgata cagcgcggtt tccaaaggaa actgcaatgc
     7921 ctgtccgcct tcgatgtgcc agctccggta ggtcagcggc ggagaaatgc cgtctgcacc
     7981 tttcaggtgg aaaccgcacg cgcccagcat cagcactgcg gcagtaagaa acagtttgtt
     8041 catgacttgc ctttctcaaa agcacaaaat cggtagatta tatcagcggc gtgttcagac
     8101 ggcattgccg tccagccaaa tcaggctcgc catacgccct gtcgctccgt cgcgtcggta
     8161 ggaaaagaaa gtgtcccgtt ccaaaaccgt acaatgcgtg ccgccgtaca cgccgcccac
     8221 gccttcgcgc ttcagaatca ggcgggcgag cgcgtaaagg tcggcaagga atttgccgcc
     8281 gcctatgcct tcaaatgcgg tggcggcttc gggcatgggc gtgcagaacg catcaaacac
     8341 atcctgtccg acttcaaacg catccgcact gatggcgggg ccgagatacg ccataatttc
     8401 gacgggcgga accttcatcg cggcaatggt gttttgcaat acgccgcccg ccaaaccgcg
     8461 ccagcccgcg tgtgcggcgg caaccgccgt accagccctg tcgcaaaata gaacgggcag
     8521 acagtcggca gtcatcacgg cacagacggc tttactcgtg tcgtccacgg aagcgtcggc
     8581 atcgggtgta ccgcctaacg tttcggcagc attgacgaca atggtgctgt ggatttgatt
     8641 gaggtaggca acgggcaatc cgacctgctg ttgcacgatt tcgcggttgc ggcgcacggc
     8701 ttcgggattg tcgccgacgt gcgaacctaa gttcaaactt tgatacgcgc cctggctcac
     8761 gccgccgttg cgcgtggtaa tcagggtttt cacattggtg ggcgcgggcc aatcggcggt
     8821 caggaagttt ttgcctttcg gggcaagatt tagggtttct gtgatggttt tcattggata
     8881 ttggaatagg tgtgtggcgg aaagggttga ttaactgcct tcggggcaaa tggatgcttg
     8941 ttttttcgtc attcccgcac aggcgggaat tcggagattc gggattgttt tcaaacgttc
     9001 gggttcggat tcttgccggt gtgggaatga tgtccttata taccttctcc gagctttata
     9061 tagtggatta acaaaaacca gtacggcgtt gcctcgcctt agctcaaaga gaacgattct
     9121 ctaaggtgct gaagcaccaa gtgaatcggt tccgtactat ttgcactgtc tacggcttcg
     9181 tcgccttgtc ctgatttttg ttaatccact atatgtttca acaggggatg gcacatcaag
     9241 caccgcactg cgtgttgccc gaacgagact accctccgat tagattctat cgccataaac
     9301 agacgggttt caaccgaaaa ggaatgggaa taaagtccgt ttccgacacc tctcgggcga
     9361 taccgtctga aaaccaatct ccactttcag acggcattgt ttaggcacgg ccattttgca
     9421 aattaaggtt aaggcgtgta tgcttgcctt cccttaaaaa caaacacgca acgcattatt
     9481 atgaatatcc tcagtaaaat cagcagcttt atcggaaaaa cattttccct ctgggccgcg
     9541 ctctttgccg ccgccgcttt tttcgcgccc gacaccttca aatgggcggg gccttatatt
     9601 ccttggctgt tgggcattat tatgttcggt atgggtttga cgctcaaacc ttccgacttc
     9661 gatattttgt tcgaacatcc caaagtcgtc atcatcggcg taatcgcaca attcgccatt
     9721 atgccggcaa ccgcctggct gctgtccaaa ctgttgaacc tgcctgccga aatcgcggtc
     9781 ggcgtgattt tggtcggctg ctgcccgggc ggtacggctt ccaatgtgat gacctatctg
     9841 gcgcgcggca atgtggcttt gtcggttgcc gttacgtctg tttccaccct gatttcccca
     9901 ttgctgactc ccgccatctt cctgatgctt gccggtgaaa tgctggaaat ccaagcgggc
     9961 agtatgttga tgtccatcgt caaaatggtt ttgctcccca ttgttttggg tttgattgtc
    10021 cataaggttt tgggcagtaa aaccgaaaag ctgaccgatg cgctgccgct ggtttccgtt
    10081 gccgccatcg tgctgattat cggcgcggtt gttggggcaa gcaaaggcaa gattatggaa
    10141 agcggcctgc tgatttttgc ggttgtcgta ctccacaacg gcatcggcta cctgctcggc
    10201 ttctttgccg ccaaatggac cggcctgcct tatgatgcac aaaaaacgct gaccatcgaa
    10261 gtcggtatgc aaaactcggg cctggccgcc gcgcttgccg ccgcacactt tgccgccgcg
    10321 ccggtcgttg ccgttccggg cgcattgttc agcgtgtggc acaatatctc cggctcgctg
    10381 ctggcaactt attgggcggc caaagccggt aaacataaaa aaccctaaac gcctttcatc
    10441 ataatgccgt ctgaacgggg caaacaaaac tggcggtttc tgcccggccc cttcagacgg
    10501 cctttgtttg aaatcggcgg ggacgattgc ccggctgctc cgattcggct gcctgcgttc
    10561 gccccgcctt tcaagccgcc tcactccctt acataaacca cttcgacatc gtagtcgtct
    10621 tcgttccaat cgtcatcgtc gtccgcgccg aatttgtcct gccattcctc ttcattgctc
    10681 aaagacgaat ccaaaccggc ttcaagacgg aggacagaca acaaatgata tatgtcgtcc
    10741 ggaatcggtg cttcaaacga aacggtttcg ccggttttcg gatgagtgaa acttaagcgg
    10801 taggcgtgca atgcctgacg cgcacccaaa cttttaacgg cttctttcac cgtgtcgccg
    10861 cacggatggc gcgggttgcc gtaaaccggg tcggcggcaa gcggatggtt ggcctcacgc
    10921 atatggacgc ggatttggtg cgtcctgcct gtttcgagcg agcattcgat atagctgtgc
    10981 gccagatagc gttccaacac tttgacgtgg gtaacggctg gtttgccgcc gaatttgacg
    11041 actgccattt tcaggcggtt gtgcggatcg cgtccgattt gggtttcgat tttaccgtcg
    11101 aaggggacga tgccattggc gacggcgcgg tagatgcgtt tgaccgtgcg ctcttgaagc
    11161 tgcctcacga gggaattttg cgccgacagg gttttggcaa ccaccatcag cccgctggtt
    11221 tctttgtcca aacggtgtac gatgcccgcg cgcggaattt gggacagctc ggggcaatgc
    11281 gccaacaggc cgttgagcag cgtccccgtc cagttgcccg ccgccggatg caccaccagt
    11341 ccggccggtt tgttgacgac gatgacggta tcgtcttcgt aaacgatatc caaagccata
    11401 ggctctggaa caaacgccag attttcctca ctcggacgga cggttacaca aatttgctcg
    11461 ccgcctatca ttttgtcttt gggttgcgaa ggtttatcgt ttacaataac cgcgccttct
    11521 ttaatccatg atgtcaggcg gctgcgcgag tagtcgggca gaagtttcgc caacaccgca
    11581 tccaaccgcc cgccggcaag ctcgagcgga acggtcagac cgatacggtt ttcagtttcg
    11641 gaagctgacg caaagtctga atcatcgcta taatcggatt cattatcaaa ggaagtattc
    11701 tgcatgaaaa aaattctttt aacggtttca ttaggtttgg cactgagtgc ctgtgccact
    11761 caaggtacgg tcgataaaga tgctcagatt acccaagatt ggagtgtgga gaagctctat
    11821 gccgaagccc aggacgagct gaacagcagc aattatacgc gagctgtcaa attatacgaa
    11881 atcttggaat cgcgcttccc taccagccgc catgcccggc aatcccaact ggataccgca
    11941 tacgcctatt ataaagacga tgaaaaagac aaagctctgg cggcaatcga acgcttccgc
    12001 cgccttcacc cgcaacaccc gaatatggat tacgcgctgt atctgcgcgg cttggtgctg
    12061 ttcaacgaag accagtcctt cttgaacaaa ctggcctcgc aagactggtc cgaccgcgac
    12121 ccgaaagcca accgcgaagc gtaccaggcg tttgcggaac tcgtccaacg cttccccaac
    12181 agcaaatacg ccgccgatgc aaccgcacgc atggtcaaac tggtcgatgc actgggcggc
    12241 aatgaaatgt cggtggcgcg ctactacatg aaacgcggtg catacatcgc cgccgccaac
    12301 cgcgcccaaa aaatcatcgg cagctaccag aatacgcgtt atgtcgaaga atcgctcgcc
    12361 atattggaac ttgcctacca aaaactcggc aaaccacagc ttgccgccga tacgcgccgc
    12421 gtgttggaaa ccaacttccc gaaaagcccg tttttgacgc acgcttggca gcccgacgat
    12481 atgccctggt ggcgttactg gcattaagcc catcttcgta atgccgtctg aagccgtttc
    12541 agacggcatt ttatgtttca aacctcattc aaacggtttt ttctgccaat agaatttata
    12601 ccctctcaaa cgttgagcct tcacgccgcc gcgtcccaag ccgaattgca gtgcgcccga
    12661 caaatcggta cgcagcagtt tgatgccgtg tgcgcggaca cggttctgca ccgcttcggt
    12721 cggatgtttg taggcgttgg cataaccgct tgaagcaacg gcatattcgg gcgagacggc
    12781 attgaggaac acgcccgacg aggacgtatt gctgccgtga tgtcccaaca ccaaaacctg
    12841 gctgtacagg ttgcctccat acttgccgac caggctttcc tcgcccttcg tatccaaatc
    12901 gcccgttacc agcagtgccg caccgcccgc cacaacacgc aaaacacaac ttttcccatt
    12961 atcatcgatg tttttgcgtt cagacggcat caaaaactca aaatccacgc cgtcccattg
    13021 ccaacgctgt tccgcacaat gccgcgcccc ctcataaaat tccggctgtc cggcataaat
    13081 cccgccgttc ggtatcttgc cgactgcctg aaaaccgccg tcgtggtcgc tgtcgtgatg
    13141 cgacagaacc agcttgtcca aacggcggac acccgccgca ttcaaactcg gcacaatccc
    13201 catctgcgcc gccgctgccg ttccggtgtc aaacaaaaga tgatgatttg ccgtctgaac
    13261 cgacaccgac aaaccctgcc ccgcatccca aaccgtaacc gcaacctcat tttccggcac
    13321 gccgggtgaa cggtaaaaca caaaccctgc caacagcaac accgcccacg gacgcaaacc
    13381 caagccgcgc ggcagcagca acagcaaagc ggcacacacc gccaacacca acagcggcaa
    13441 aggtgcggag gcaacggcaa actcgggcga cacatcggca agccacacca aaaaccgcaa
    13501 agtatattcc gccaaaaatg cccccaactg ttgcaaaggc gcaaacggca cgaccgaacc
    13561 cagcaacgcc agcggcgtca atacccaaga aaaccacgga attgccaccg cattgaccaa
    13621 agggctgacc aaaggcagcg aagcaaacag ataaccgagc aaaaccagcg acaacaccga
    13681 agccgcccac tgcccgcgca aagcagtttg ccgttttccc tcgtgcaaac gccccgagca
    13741 cgcccatatc agggccgcca ccaaaccgaa agacagccaa gtccccacac ccaagaccgc
    13801 cgacgggtcg aacagcagca ccgccgccaa cgcctgccac cacgtcgccc acgccgacaa
    13861 tcttcccctg cgccaagccc acgcaaacgt cgccaacatc aaaacgctgc gctgcgtcgg
    13921 cacggaaaaa ccggcaagca gcgcgtaaaa cagcgcgcct gcacaccccg ccgccaaaac
    13981 ccacgcgcgc ggccgggcag gaaggcgcgg ggaacaggca agcagccgct tcgccagcca
    14041 cgcaaacatt accgccacca tcgtaacgtg caaacccgaa atactgacca aatgcgtcag
    14101 ccccaacggc cggaacgcct gccacaattc ggggcgcaat gccgactgtt cgcccacgct
    14161 caacgcgcgc atcagcccga tgccgtctga aagcccgccg tccgcatccg cctgctgcca
    14221 attacggctg atgcggctgc gccaaaccgc aatcccccaa ccgcttccgc catgcagcaa
    14281 aaccctgtcc gcaccgaccg tccccgcgcc gcctattccg ttggataatg cccacgcctc
    14341 acggttcaaa ccgcgcaggt tcaactcccc gacgacaggg tgcacacgtg ccgttatccg
    14401 ccatctgctc ccgaccgccc attcgcgccg tttgtagtcc gacagcagca aatcaaacgt
    14461 ccgaccaccg ctgtccacag cctttgccgc aaactgcaca cgccgcccgt ccgacctcgg
    14521 catatccgcc acttccaccg tcaaaggcac gcctgaaacg gcttctgccc accattggga
    14581 agacaatgcc gcctccgtcc tgaatacgcc gtaagccgcg cccgccaaca cgcacagcat
    14641 cagaccggca aacgcaaaac gccttgcaaa cacagcaaaa accgcaaaag ccgccagcca
    14701 aaacagccaa tgcggcacga ccggcagcgc gaacgaagcc gccgcaccga caacccaaca
    14761 cggcaaaaac caacgacgga acacgtctcc ccaccccaga tatttcaaaa ataaacggat
    14821 aataaagcaa atccgaaaaa ccgtcataac aagatttgaa aatgatatag tggtttaaat
    14881 ttaaatcagg acaaggcgac gaagccgcag acagtacaag cagtacgaca aggcgaggca
    14941 acgctgtact ggtttttgtt aaaccactat aagaatgcaa acgaaaagcc tcagtattat
    15001 ccgagacaac ggcaaacttt ccatcgtcat tcccgcacaa gcgggaatcc aaaaacaaaa
    15061 agccgcagaa atttaacgga agaaccaaac cccgaaaacc ggctccccgc cttcgcttga
    15121 ataacagaac aaaggaatca gtattatccg agacaaccgc aaactttcca tcgtcattcc
    15181 cgcacaagcg ggaatccaaa cttgtccgca cggaaactta tcggataaaa cggtttctta
    15241 gattccacgt tctagattcc cgc
//
LOCUS       DAWFLT010000046        15080 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWFLT010000046 DAWFLT010000000
VERSION     DAWFLT010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15080
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.046"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(664..948)
                     /locus_tag="WGR74_001363"
     CDS             complement(664..948)
                     /locus_tag="WGR74_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0905771.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
     gene            complement(1537..2802)
                     /gene="ftsY"
                     /locus_tag="WGR74_001364"
     CDS             complement(1537..2802)
                     /gene="ftsY"
                     /locus_tag="WGR74_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ0905772.1"
                     /translation="MFSFFRRKKKQETPAPEEAQIQETAAKAESETAQVIENIKEDVN
                     SLAESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAVAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
     gene            2948..4516
                     /gene="msrAB"
                     /locus_tag="WGR74_001365"
     CDS             2948..4516
                     /gene="msrAB"
                     /locus_tag="WGR74_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ0905773.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSAIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            complement(4618..5103)
                     /locus_tag="WGR74_001366"
     CDS             complement(4618..5103)
                     /locus_tag="WGR74_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ0905774.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(5129..5977)
                     /locus_tag="WGR74_001367"
     CDS             complement(5129..5977)
                     /locus_tag="WGR74_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0905775.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            complement(6071..7072)
                     /locus_tag="WGR74_001368"
     CDS             complement(6071..7072)
                     /locus_tag="WGR74_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0905776.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPGSAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            7131..7844
                     /locus_tag="WGR74_001369"
     CDS             7131..7844
                     /locus_tag="WGR74_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ0905777.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGIFTLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMADTENTLCR
                     LNGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKVHDLPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTRTTPENTKECYA"
     gene            7837..8109
                     /locus_tag="WGR74_001370"
     CDS             7837..8109
                     /locus_tag="WGR74_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HEZ0905778.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            8164..9534
                     /gene="glmU"
                     /locus_tag="WGR74_001371"
     CDS             8164..9534
                     /gene="glmU"
                     /locus_tag="WGR74_001371"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ0905779.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHCIGGKPMVERVIDTAA
                     ALNPQNICVVIGHGKEQVLNTVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPTGLGRIIRDGNGSVTAIVEEKDADAA
                     QKAVKEINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRA
                     SYLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDAN
                     CIFEGEIELGDNVEIGANCVIKNAKIGANSKIAPFSHLEDCEVGENNRIGPYARLRPQ
                     AKLANDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNIEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            9610..9938
                     /locus_tag="WGR74_001372"
                     /pseudo
     CDS             9610..9938
                     /locus_tag="WGR74_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
     gene            complement(10010..11275)
                     /gene="efeB"
                     /locus_tag="WGR74_001373"
     CDS             complement(10010..11275)
                     /gene="efeB"
                     /locus_tag="WGR74_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ0905780.1"
                     /translation="MSTNNPTQPTRRTLFKTALAAGVGGAIGGYLGGKGLGETAERTA
                     ESQHSPQAYPCYGKNQAGIVTPQQAFSIMCAFDVTAQNAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(11419..12585)
                     /locus_tag="WGR74_001374"
     CDS             complement(11419..12585)
                     /locus_tag="WGR74_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ0905781.1"
                     /translation="MRKFNLTTLSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLSEADRKALQAPINALAEDLAQLRGILGLK"
     gene            complement(12582..13421)
                     /locus_tag="WGR74_001375"
     CDS             complement(12582..13421)
                     /locus_tag="WGR74_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ0905782.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGIGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARLMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVITWFLCGSRPSETLTRKE
                     ELK"
     gene            13634..14959
                     /locus_tag="WGR74_001376"
     CDS             13634..14959
                     /locus_tag="WGR74_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ0905783.1"
                     /translation="MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGKYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFF
                     RELTGSGNDGPVGALGTPLMGEYAGAVDQHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
BASE COUNT         3542 a         4454 c         3771 g         3313 t
ORIGIN      
        1 aagtgggaat ccagaatctc taaagcttca gctaaccttt gaatattact gttgttctaa
       61 ggtctagatt cccgcctgcg cgggaatgac gggtctttta taacctttga atattgctgt
      121 tatcccaagg tctggattcc cgcctgcgcg ggaatgacga atccatccgc acggaaacct
      181 gcaccgcgtc attcccgcga acctacattc cgtcattccc acgaaagtgg gaatctagaa
      241 tcccggactt tcagataatc tttgaatatt gctgttattc taaggtctgg attcccgcct
      301 gcgcgggaat gacggtagag cggtttctgt tttttccaat aaattcctaa aactcaaaat
      361 ttcatcattc ctacaaaaac agaaaaccaa aaacagaaac ctaaaatccc gtcattccca
      421 cgaaagtggg aatccaggac gcggaatctc aagaaaccgt ttacccgata agtttccgtg
      481 ccgacagacc tagattcccg cctgcgcggg aatgacgggt cttttataat ctttgaatat
      541 tactgttgtt ctaaggtcta gattcccgcc tgcgcgggaa tgacggttta gaagttgccc
      601 gaaacctcaa aaaaaaaacc gaaaccgaac aagccggatt cccgcctgcg cgggaatgac
      661 gggctaaata atatcaaacc ataaatcctg ccaagaaaca ttattttctt caatcagttg
      721 caatttccaa gccctgttcc atttcttcaa ctgtttttcc cgagtaattg cactctccat
      781 cgtaggatgc agttcatacc aaaccagcat agtaacgttg taccgtgatg taaatccctc
      841 aatcaaatgc tccctatgtt ggtaaatacg ttgcaccaaa tcagatgtaa cgccaatgta
      901 taacgtgcca ttacgttggc ttgctaaaat ataaaccgca ggctgcatat aatacccttt
      961 tgaattattt caatttatat tcccgcgaac accatcccgt gattacttta acccttcgtt
     1021 attcccatag ctttccatca ttcccgcaac tcttcgtcat tcccgcgaaa gtgggaatct
     1081 agaacgcaaa atctaaagaa accgttttac ccgataagtt tccgcaccga caaacctaga
     1141 ttcccgcctg cgcgggaatg acggcggagc ggtttctgtt ttttccgata aattcctaaa
     1201 actcaaaatt tcatcattcc tacaaaaaca gaaaaacaaa atcagaaacc taaaatcccg
     1261 tcattcccac gaaagtggga atctaggacg cggaatctca agaaaccgtt tacccgataa
     1321 gtttccgtgc cgatggttct agattcccgc ctgcgcggga atgacaggtc ttttataaac
     1381 tttgaatatt gctgttatcc caaggtctgg attcccgcct acgcgggaat gacgggtctt
     1441 ttataaactt tgaatattgc cgttatccca aggtctggat ttccgcctgc gcgggaatga
     1501 cggcatcggt ctgctgtttt cggacggcat ttcggctcaa tccagcagtg cgtccacaaa
     1561 cgcgcgcgcg tcaaacgggc gcaaatcgtc tatgccttcg cccacgccga tatagcggac
     1621 ggggacgggg cggtcggaag caagcgcggc gaggatgccg ccttttgccg tgccgtcgag
     1681 tttggtaacg ataagccccg tcagccctaa tgcgtcgtca aaggctttga cttggttgac
     1741 ggcgttttgc ccgatgttgg catcaagcac gacgatgatt tcgtgcggcg cgtcgggcat
     1801 ggctttttgc agcacgcgtt tcactttttt gatttcttcc atcaagtgaa gctgcgtggg
     1861 caggcggccg gcggtgtcgg caagcacgat gtcgatgccg cgcgctttgg cggcttggac
     1921 ggcatcgaag cacacggctg cggaatcgcc cgtggtttgt gaaatcacgg ttacgttgtt
     1981 gcgttcgccc caagcttgaa gctgctcacg ggcggcggcg cggaaggtgt cgccggcggc
     2041 aagcagcaca gatttgccct gcgcttggaa atatttggcg agtttgccga tagacgtggt
     2101 tttgcccgcg ccgttgatgc cggcaagcat gatgacaaac ggctctttgg tttcgggcaa
     2161 gaccaatggt ttttccagag gcttaatcag gtcatacaag gcttctttca acgcgccgcg
     2221 caattcgttg ccgtctttca gccctttgag gctgacgcgg tcgcgcacgt ctttcatcag
     2281 gtattcggtg gcttccatgc ccatgtcgct ggtaatcagc acggtttcca gctcttcgta
     2341 caaatcttcg tcgatttgtc cgccgccgaa cacgcccgcc agcgatttcg ccattttgtc
     2401 gcgcgatttg gtcaaacctt gtttcaaacg cgccgcccaa ccgagcttgt gttcttcagt
     2461 tgtcgcaacg gcttcttgaa cttgcccgac agcctcgccg acggtttcgg caacggcttc
     2521 ttttgccgct tcgacttgtt ccgctgcttt ttccgccgct tcctctgctt cagacagcat
     2581 ctcggcaacg gtttccttta cctgttcaac cgcaccgctg acggtttcaa cggcagattc
     2641 gacctgccct ttgacgcttt ccgccaaaga attaacatct tctttaatat tttcaattac
     2701 ttgagcagtt tcggattctg cttttgctgc ggtttcctga atttgagcct cctctggagc
     2761 cggcgtttcc tgttttttct tgcgacggaa gaagctgaac attggatttt ccttttaatt
     2821 ttagaaactt gaaatagggc gtattgtagc gtattttacg cggcagggtt gtctgaaaat
     2881 ccgggctgta aggtttcggc atctcaaacg tctaatcatg caaaccgtcc acacaggaaa
     2941 catcaaaatg aaacaccgta ctttcttttc cctttgcgcc aagttcggct gcctgcttgc
     3001 gctgggtgct tgttcgccca aaatcgtcga tgccggagcc gcgaccgtgc cgcacacttt
     3061 atccactttg aaaaccgcgg acaaccgccc cgccagtgtt tacttgaaaa aagacaaacc
     3121 gacgctgatt aaattttggg cgagctggtg tcctttgtgt ctgtccgaat tgggacagac
     3181 cgaaaaatgg gcgcaagatg caaaattcag ctccgccaac ctgattaccg tcgcctcccc
     3241 gggctttttg cacgagaaaa aagacggcga ctttcaaaaa tggtatgccg gtttgaatta
     3301 tcccaagctg cccgtcgtga ccgacaacgg cggcacgatt gcccaaagcc tgaatatcag
     3361 cgtttacccc tcatgggcgt taatcggtaa agacggcgac gtgcagcgca tcgtcaaagg
     3421 cagcatcaac gaagcgcagg cgttggcgtt aatccgcgac ccgaatgccg atttgggcag
     3481 tttgaaacat tcgttctaca aacccgacac tcagaaaaag gattcagcaa tcatgaatac
     3541 acgcaccatc tacctcgccg gcggctgctt ctggggcttg gaagcctatt tccaacgcat
     3601 cgacggcgtg gttgacgcgg tatccggcta cgccaacggc aacacgaaaa atccgagcta
     3661 tgaagacgtg tcctaccgcc atacgggcca cgccgaaacc gtcaaagtga cctacgatgc
     3721 cgacaaactc agtctagacg acatcctgca atatttcttc cgtgtcgttg atccgaccag
     3781 cctcaacaaa cagggcaacg acaccggtac gcaataccgc agcggcgtgt actacaccga
     3841 ccccgccgaa aaagccgtca tcgccgccgc cctcaaacgc gagcagcaaa aataccaact
     3901 gcccctcgtt gttgaaaacg agccgctgaa aaacttctac gatgccgagg aataccatca
     3961 ggactacctg attaaaaacc ccaacggcta ctgccacatc gatatccgca aagccgacga
     4021 accgctgccg ggcaaaacca agaccgcccc gcaaggcaaa ggcttcgatg cggcaacgta
     4081 taaaaaaccg agtgacgccg aactcaaacg caccctgacc gaagagcaat accaagttac
     4141 ccaaaacagc gcgaccgaat atgccttcag ccacgaatac gaccatttgt tcaaacccgg
     4201 catttatgtg gacgttgtca gcggcgaacc tttgttcagc tccgccgaca aatatgattc
     4261 cggctgcggc tggccgagct tcacgcgccc gattgatgca aaatccgtta ccgaacacga
     4321 tgatttcagc tacaacatgc gccgcaccga agtgcgcagc cacgccgccg attcgcactt
     4381 ggggcacgtc ttccccgacg gcccgcgcga caaaggcgga ctgcgctact gcatcaacgg
     4441 cgcgagcttg aaattcatcc cgctggaaca aatggacgcg gcaggctacg gcgcgttgaa
     4501 gagtaaagtg aaataagccg caccgccgcc taacccgaca aaataccgtc tgaaacctgc
     4561 aacgtttcag acggtatttt ttatccggcg gggattctgt ccagacggag attttgttta
     4621 gacggcatcg ccgccgtttt caatcagccc cgccaaccgt tccaatgcga aggcgaccgc
     4681 ctgcgcacgg acggattcgc ggttgccgtc aaaacggcgc attgcttcgc aacttccgcc
     4741 tggaaaggca aacccgaacc aaaccgtgcc gacgggtttg ctttcgctgc cgccgcccgg
     4801 gccggcgatg ccggaaatac cgacggcgta atccgcctgc gccacggctt tcgcgccgcg
     4861 cgccatctca tagacggttt ggcggctgac cgcgccgtgt tcgagcaggg tttcgggcaa
     4921 cacgcccaag cggtcttctt tggctttgtt gctgtatgtt acaaaactct ggtcgaacca
     4981 ttgcgaactg cctgcaacgc ttgtgaatgc ggcggcaagc attccgcccg tacaggactc
     5041 ggcacagctt acggtttgac gttttttcgt caggtttcgg gcgatggtgt gcagcgcgtc
     5101 catttcccac tcccctttca gacggcgttt aagaattgat gatgtgtatg tcgcgttgcg
     5161 ggaacgggat gttgatattg actttgcgga ggttttcgac cacttgttcg ttcaagtcgc
     5221 attgcagcgt ccagcggtct gcttcgtttg cccaagccca taatgtgatt tcgatggcat
     5281 tgtcgcccaa ggcggtgatg taggcggcgg cctgccgctc ttcgttttga acgctcaagg
     5341 ggtgttcgac ggcggctttc aacaccgcct ctttcgccac tttcaaatcg cagttgtaat
     5401 cgacgccgac tatcacttgg gcgcggcaca gcggcagtgt ggaacggttg acgatgctgt
     5461 tgcccatcac cacgctgttg ggcagcacga cttcttcgtt gtcggtcgtc cgcaaagaag
     5521 tctgcaccat tttaatctct cggacatatc cttcaaaacc gccgacgcgg ataaaatcgc
     5581 cgactttgaa cgggcggaac aggataatca gcgcgccggc ggcaaaattg gacagctggt
     5641 ctttcaggga caacgccacc gccaaacccg cgccgccgat taaggcggtt acggatgttg
     5701 tggaaacgcc caatctgccc aatgcggcaa taatcaccaa aatcaataag ccgatattgg
     5761 caacattaca caaaaaacta atcagcgtgg catcgacctg cgcgcgcgtc atcgccgccc
     5821 tcatcacagc gacaatgcgt ttcgccgccc attttccgac caaaaaaata agcagcgcgg
     5881 cggcaaggtt cagcccgaac gcccacgcct tttcagccag atgctcccaa ccggaaacac
     5941 tgatcaggtg taaaaaatca aattgtttga agtccattgt tttcctcttg atcgaacatc
     6001 cgccccgtgc ggcgtaatcg gcacaggtgt aaaaatgccg tatgaagccc tgcggggcgg
     6061 tatgtttgtt ttatttcaaa accgttctaa tccaacggga aacccatccg cgctggttcg
     6121 catcaatatc gcgctgcgcg ggggcggtgg tgtgcgtcgg ggcttgggcg aagcggaaca
     6181 cgtcgggcag gcgcgtgttt ttgacggcgg ggtaaaccca catttcggac ggaaccgcct
     6241 gctgcacttc ccgactttgc agccattgca ccagttttgc cgccagcttc ggctgtttcg
     6301 cgcccttcaa gactgccgcg ccttcgacct ggcggaatac gccgcctttt aaaaacaggt
     6361 tgcccgtcgg cggctcgctg tatttgcctt tggaaaaata cacttccgcc gccgggctgg
     6421 cggcataacc gaccaccagc ggatacgcgc cgccgttgtg cgaaaagtcg gtgtaatacg
     6481 cctcgctcca gcctttggcg accttcacgc cgttctgccg catctgtgcc caccatttga
     6541 acgcgccttc ttcgcccaaa ccgctgatgt tcgccatcag gaagcccagc cccggggacg
     6601 acgtggcggg agacggcacg accaataggt ttttatattc ggggcgggtc aaatcctgca
     6661 gggtttgcgg cagaggaagc tttttgcctt caaaccattt tttgtcgtaa ttgatggaca
     6721 catagccgta atcgaccgcc aaagccgaag gcagcccgac cgcgacgggg gcggatccgg
     6781 gttgcgccgc cgccaaaatg cccatttccc gcgccttgcc gatattggcg ttgtccaagc
     6841 cgtacaccgc gtcggcgatc gggttggcgc ggctcaaaat cagtttgttg agcatttcgt
     6901 tcgcgccgcc cgcctgaata atcgacacct tcgcatcgtt tgcccgctcg aagcgcgcaa
     6961 tcagcccttt gggcaggctg aacgacttat gcaccgccag cctgacttcc gtctgcgcct
     7021 gcaggtatgc cgaaaccgcc agcagcggca gcagccaaat tttccgtttc attccgagtc
     7081 ctcctctatt cgctgtaaaa taacattcta acaaattttc acggttcaaa atgcaagaaa
     7141 acccgaccgt gtggctgttc gacctcgaca acacgctgca caatgccgat gccggcatct
     7201 tcacactcat caaccgcgcc atgacacgct atatggcacg ccgcctcaaa ctctccgaat
     7261 ctgccgcgtc cgacctgcgt caagactatt ggcaccgcta cggcgcaacg ctcgccggac
     7321 tgcaaatcca ccatcccgaa atcgacatcg ccgaattttt gcgcgaaagc catccgatcg
     7381 atgcaatcct gaccaggctg cacggcatgg cggatacgga aaacacccta tgccgtctga
     7441 acgggcgcaa ggcggttttt tccaacggcc cgtcgtttta cgtccgtgcc gttgtcggcg
     7501 cactcggttt ggaaaaccgt ttcgacgcac tgttcggcac ggatgatttc gggctgctgt
     7561 acaaacccaa tccgcaagcg tatctcaatg tctgccgcct tttggacgta ccgcccgaat
     7621 gctgcattat ggtggacgac agcgcggaca acctgcatca ggcaaaagca ttgggcatga
     7681 aaaccgtccg gttcggtgca aaagtgcacg acctgccctt tatcgatgct tccgtaagcg
     7741 atatggcgca actggctcgg tatgcagaaa ctttgtcaga acaccgccaa aatcattaca
     7801 atactcgcac aacccccgaa aatacgaaag aatgttatgc gtaaaacctt ccttatcctg
     7861 atgactgccg ccgccctttt gtcgggctgc gcgtgggaaa cttatcaaga cggcaacggc
     7921 aagaccgccg tccgtcaaaa atatcccgcc ggcacgcccg tttattacca agacggcagc
     7981 tactcgaaaa atatgaacta caaccaatac cgtcccgaac gccatgccgt gttacccaac
     8041 caaaccggca acaacgccga cgaagagcat cgccaacact ggcaaaagcc caaatttcaa
     8101 aaccgataaa cccaccctat gccgtctgaa gccctttcag acggcattgc acaggaaaac
     8161 gctatgccgc aaaacacttt aaacatcgtc atcctcgccg ccggcaaagg cacgcgcatg
     8221 tattccaaaa tgcccaaagt gctgcactgc atcggcggca agcccatggt tgagcgcgtt
     8281 atcgacaccg ccgccgcact gaatccgcaa aacatctgcg tcgtcatcgg acacggcaaa
     8341 gagcaagttt tgaatactgt caaacgcgat gtcgtttggg ttgaacaaac cgaacagctc
     8401 ggcaccggcc acgccgtcaa aaccgccctg ccccaccttg ccgccgaagg ccgcacgctg
     8461 gtgctgtacg gcgacgttcc tttaattgac gttgaaaccc tcgaaaccct gctcgaagcc
     8521 gcaggcaacg aagtcgggct gttgaccgac gtgcccgccg acccgacagg cttggggcgc
     8581 atcatccgcg acggcaacgg cagcgtaacc gccatcgtcg aagaaaaaga cgccgatgcc
     8641 gcccaaaaag ccgtgaaaga aatcaacaca ggcatcctcg tcctgcccaa cgccaaactg
     8701 gaaaactggc tgaacagcct ctccagcaac aacgcacaag gcgaatacta tctgaccgac
     8761 ctcatcgcca aagccgttgc cgatggtatt aaagtccatc ccgtccaagt gcgcgcctcc
     8821 tacctcgccg ccggcgtgaa caacaaacgc cagctcgccg aactcgaacg catcttccaa
     8881 accgaacagg cgcaagaatt gctcaaagca ggcgtaaccc tgcgcgatcc ggcacgtttc
     8941 gatttacgag gccgtctgaa acacggacag gatgtcgtga ttgatgcaaa ctgtatcttt
     9001 gaaggcgaaa tcgagctcgg cgacaacgtc gaaatcggcg caaactgcgt catcaaaaac
     9061 gccaaaatcg gtgcaaacag caaaatcgcc cccttctccc acctcgaaga ctgcgaagtc
     9121 ggcgaaaaca accgaatcgg cccatacgcc cgtttgcgtc cgcaagccaa actagctaac
     9181 gacgtacacg tcggcaactt cgtcgaaatc aaaaacgccg ccatcggcaa aggcaccaaa
     9241 gccaaccacc tcacctacat cggcgatgcc gaagtcggca gcaaaaccaa cttcggcgcc
     9301 ggtacgatta ttgccaacta cgacggcgtg cacaaacaca aaaccgttat cggcgacgaa
     9361 gtccgcatcg gttcaaactg cgtcctagtc gcccccgtta ccctcggcaa caaagtaacc
     9421 acaggcgcag gcagcacgat tacccgcaat atcgaagaca acaaactcgc cctcgcccgc
     9481 gcccgccaaa ccgtcatcga aggatgggtg aggccggaaa aagacaaaca ataaagccat
     9541 gccgtctgaa gccggtttca ggtttcagac ggcatcccaa aaacaaacat ccgataagga
     9601 cggaaaacca tgtcattacc cccatgcccg caatgcgcct ccgaatacac ctatgaagac
     9661 ggcggacaat acatctgccc cgaatgcgcc cacgaatgga atgaaaccga atccgccgcc
     9721 gaccttgcgg ctcaagtgcg cgatgccaac ggcgcggcac tgcaaaacgg cgataccgtc
     9781 atcctcatca aagacctcaa ggtaaaaggc agctcgatgg tgatcaaaca aggtacaaaa
     9841 gtcaaaggca tacgcctgca ggaaggcgat cacacatcgg ctgcaaaatc gacggcagcg
     9901 cgatgaattt aaaatccgaa ttcgtcaaaa aagcctgacc gcccaaaaac aagaaacgcc
     9961 gtccgaaccc gtttggcaag gttcggacgg cgttttttat atggcggatt tatacgccca
    10021 gcagcccttg ccccaaaaag ccgccttttt ccacgccggg caagacgaag aaatagccgc
    10081 cgccgaaggg gctgatgtat tcttccagcg gttcgccgtt gaggaggttt tgcacgaaga
    10141 tgaatccgtc ggcaaggttt gcctgatagc agacgaacac cagtccgaca tcaagctgtc
    10201 cgcttgaggc gagtccgcgc gaatagctgt aggcgcggcg gaagaggcgg tgttttttga
    10261 ggaattcggg atcgcgcgga ttcgccaggc gtatatggct gtctttgggc gtggtattcc
    10321 cctcggggtc tttggcaaaa tccggttggt cggcttcttt tttgccgtcc atcggcgcac
    10381 cgctgtattt gcgccgcccg aaaatgtcgg tttgctcttg aagcggcgtc ctgtcccaaa
    10441 actcgacaaa gtggcggata aggcggactg cctgatagct gccgtttttc gcccactccg
    10501 gttcgtcgag gctgttggcg gccacacccg tccacaaaac ctcgtcggcg gttttggggt
    10561 cggaaacttt ggggttgccc gtgccgtccc tgaagcccaa caggttgcgc gccgccatcg
    10621 cgccgggttc ggatttgggc tgccacccgt cgatactcca acggataacg gcggtttgga
    10681 cggtgtgttt gatgatgtcg cgcagggcgg cttggcaggt ttcgggggta aaggcacaga
    10741 tttgtaggct caaatcgccg tcgcaccagc ttttttgcag cttatcgttg gagaagtcgc
    10801 gcatttcctg caaatgaatc ggttttttgt ctttgagtcc gaatcgtccg tcaaacaggc
    10861 tgctgcccac ccccacggta acggtcaatc cgtcggggtt gaaggctttg cccaaaatgc
    10921 cgctgccggc cggcggaagt ttgtcgtcgc cgtcttggta ttcaccgcct tgagtgagaa
    10981 actcgatacg ggcagtcagc gtgcggaaca ggttttccag ctgcttggcg ttttgcgcgg
    11041 taacgtcaaa ggcgcacatg atggaaaatg cctgctgcgg cgtaacgata cctgcctgat
    11101 tctttccgta acagggatag gcttggggcg agtgttggct ttcggcggtg cgttcggcag
    11161 tttcacccag ccccttccca ccgagataac ctccgattgc gccgcccacg ccggctgcga
    11221 gtgcggtttt aaagagggtg cgcctggtcg gttgtgtcgg gttgttcgta ctcatgtttt
    11281 tatcctttat ttaaactcgg tatccgatac ggcggaacgc gggaaacgga aacctccgcc
    11341 tttttcaata cctttccggt tttgcagata aaatttcaga cggcatctgt cgctaaatgc
    11401 cgtctgaacg cttaaggctt atttcaagcc gagtatgccg cgaagttggg caaggtcttc
    11461 ggcaagcgcg ttaatagggg cctgtaacgc tttgcggtcg gcttcgctca gcttgtcgta
    11521 ggtttcaaaa ccgtctttgg tgcggtattt cgccagaatt tcgttgacct gtttgaagtt
    11581 ggtatcggtt ttttccaaca aggctttgtt tttggcctca atcagcggac ggaacaaatc
    11641 gacgattttt ttcgaaccgt ccacattggc ttggaagtcg ctcaaatcgg tgtggctgta
    11701 ccggtcttct tcgccgctga ttttactgcc cgccacttct tcaatcagtt cggacgcgcc
    11761 gccgaccacc ttgcccggag gaaacgccaa tgcgtcgatt tctttttgca gggcttcgac
    11821 atcggtcatc agtttcgctg caatttcctt cacgccggac acgtcttttt ccacccaaag
    11881 ggcgtattcg atacggtgaa agccggtaaa tccggcatct ttcgcgccgt ctttgaagtc
    11941 gtcttcacgc gcatcgatga cggggtcgag ttcgctgaaa agctcggcaa tcggttcgat
    12001 gcgttcgtaa tggacgcggg tgtcggcaaa cagggatttc gccttttcaa tgtcgcctgc
    12061 tttgacggct tcggtaaagg ttttggtttt cgccaccagc tctttaacct cgccttgaac
    12121 gtaggcttta tagtcggcga gcggttggga cagtttttcc aaatccgctt cgttggcggt
    12181 gtctttaaag ccgctgtcgg ttaccaccag cttgccgcgc ggattggtca aaagaccgca
    12241 agtcatttcg tattcgcccg gcaacagggt gacggtcatt ttatcggaaa gtccgggggc
    12301 gatgttttcg cgctcgtcca ccaccatcac gcctttcagg atttcccatt cgagcttgcg
    12361 gccgctgttg tttttaatat tgaacacaac ctgtccgctc ggcacggtca gttccatcgg
    12421 ttcgcaggca ttgtcgttga cggcgatact gaccgaaccg ccctcgttgg cggtttgcgc
    12481 ctcaccggac gcggccggcg cagctttctc cgcctccggc ggctggcacg cggtcaaacc
    12541 taaggcaagc atcacggaca atgtggtcaa attgaatttt ctcatttcag ctcctcttta
    12601 cgggttaaag tttcagacgg cctgctgccg cacaaaaacc aagttatgac gggaataagg
    12661 tacagcagcc aaaccaaggc ctcgccctgc gtcggatggt cggtatagcc gaaaaatccg
    12721 ccgagcagca cacctaacgg actgtcttcg tgcaaatatt ttgatgagtc gaacacaatg
    12781 tcctgaagcg cgttccaaat gcctgcttcg tgcagcgcgc gcagcgagcc ggcaagcagg
    12841 ccggcggcaa cgacaatcaa aaacgccccc gtccaacgga aaaacttcgc cagattcagg
    12901 cgcatcccac cctgataaat caacgcgcca atcacggcgg cagccaaaac ccccgctacc
    12961 gcaccggccg gcatctgcca cgtcgggctc tgtttgaata cggcaagcag gaaaaaaacg
    13021 ctctccaaac cttcgcgcgc cacggcaaga aacgccatac cgaccaaggc ccatccttga
    13081 ccgctgccac ggttcaaagc cgcctgtaca gaatcctgaa gctgccgctt catcaaacgc
    13141 gccgcctttt tcatccataa aatcatataa gtcagcatcg cgacagcaac caaaccgata
    13201 atgccgacga cgaactcctg ctgcttctgg ggaatctcgc ccgttgccga atggatgccg
    13261 tatccgatgc ccaaacacat caaagaagca aggacgaccc cgaaccagac cttaggcatc
    13321 agtttggaat gtccggactg tttcagaaaa ccggcaacga tgccgacgat gagcgcggct
    13381 tcgataccct cgcgcaacat aattaaaaaa gcgaccagca taaacgcgaa cgaacaagga
    13441 tgatgaataa tatattatcg gaatattttc attgcttgta aatacaaatg caagttattt
    13501 ttatctgcag taccgcgcgg cggaaagttc cgcaggctgc agctgcgccc tgtgttaaaa
    13561 tcccctctcc acggctgccg caacgccgcc cgaaaccatc ttttttttat tactgccggc
    13621 aacattgtcc attatgaaaa aatacctgtt ccgcgccgcc ctgtgcggca tcgccgccgc
    13681 catcctcgcc gcctgccaaa gcaagagcat ccaaaccttt ccgcaacccg acacatccgt
    13741 catcaacggc ccggaccggc cggtcggcat ccccgacccc gccggaacga cggtcggcgg
    13801 cggcggggcc gtttataccg ttgtgccgca cctgtccctg ccccactggg cggcgcagga
    13861 tttcgccaaa agcctgcaat ccttccgcct cggctgcgcc aatttgaaaa accgccaagg
    13921 ctggcaggat gtgtgcgccc aagcctttca aacccccgtc cattcctttc aggcaaaaca
    13981 gttttttgaa cgctatttca cgccgtggca ggttgcaggc aacggaagcc ttgccggtac
    14041 ggttaccggc tattacgagc cggtgctgaa gggcgacgac aggcggacgg cacaagcccg
    14101 cttcccgatt tacggtattc ccgacgattt tatctccgtc cccctgcctg ccggtttgcg
    14161 gagcggaaaa gcccttgtcc gcatcaggca gacgggaaaa aacagcggca caatcgacaa
    14221 taccggcggc acacataccg ccgacctctc ccgattcccc atcaccgcac gcacaacggc
    14281 aatcaagggc aggtttgaag gaagccgctt cctcccttac cacacgcgaa accaaatcaa
    14341 cggcggcgcg cttgacggca aagcaccgat actcggttac gccgaagacc ccgtcgaact
    14401 ctttttcatg cacatccaag gctcgggccg tctgaaaacc ccgtccggca aatacatccg
    14461 catcggctat gccgacaaaa acgagcatcc ctatgtttcc atcggaaaat acatggcgga
    14521 taagggctac ctcaaactcg gacaaacctc catgcagggc attaagtctt atatgcggca
    14581 aaatccgcaa cgcctcgccg aagttttggg tcaaaacccc agctatatct ttttccgcga
    14641 gcttaccgga agcggcaatg acggccctgt cggcgcactg ggcacgccgc tgatggggga
    14701 atatgccggc gcagtcgacc agcactacat taccttgggt gcgcccttat ttgtcgccac
    14761 cgcccatccg gttacccgca aagccctcaa ccgcctgatt atggcgcagg ataccggcag
    14821 cgcgattaaa ggcgcggtgc gcgtggatta tttttgggga tacggcgacg aagccggcga
    14881 acttgccggc aaacagaaaa ccacgggtta cgtctggcag ctcctaccca acggtatgaa
    14941 gcccgaatac cgcccgtaaa ccggaaaaca atgccatctg aagcccatcc ctaggcaatc
    15001 gggcgcatta aggctaagtt ttgcgatacc gcaggcacaa aattcctttc ttatttgacg
    15061 gaattatagt ggatcaaatt
//
LOCUS       DAWFLT010000047        14950 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWFLT010000047 DAWFLT010000000
VERSION     DAWFLT010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14950
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.047"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            136..2016
                     /locus_tag="WGR74_001377"
     CDS             136..2016
                     /locus_tag="WGR74_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEZ0905784.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTPHGKRTKVKANNVFAE
                     FDGDMAAFLENAQVQAADIDTDLLWEVCGGEEFAAEAIAEEYYGHTPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAETLKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGTKRVGIHIAAPS
                     LAVKPGDKMEQVIMQRLSTVYFPNGKITMLPENWIAAFSLDAGAHRPAVSIYFDVDGE
                     FNVGAPTCKIEAVNISENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDKEDNVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVRHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
     gene            complement(3250..4275)
                     /locus_tag="WGR74_001378"
     CDS             complement(3250..4275)
                     /locus_tag="WGR74_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0905785.1"
                     /translation="MKIKALMIAAALLAAADVHAAPQKAKTASAKAAKAAKAAKVAKV
                     AATAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKANLPKDAQISESE
                     LSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNSALKNLSPAQRRDFADNIIA
                     EKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKADSENAAV
                     GAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVMVPAFEEAVHALK
                     PGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAEQATVNLLRDLHS
                     GAYVDIR"
     gene            complement(4380..6788)
                     /locus_tag="WGR74_001379"
     CDS             complement(4380..6788)
                     /locus_tag="WGR74_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ0905786.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVQGSGEASIPEDYTRIVADRMEGQSQ
                     VQVRAEGNVVVERNRTTLNADWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GMTFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKNDAVMLDGSVGKKPRSRS
                     DWVAFASSGIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            6858..7862
                     /locus_tag="WGR74_001380"
     CDS             6858..7862
                     /locus_tag="WGR74_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ0905787.1"
                     /translation="MQRQTELKNWLQTIYPERDFDLSFAAADADFRRYFRATFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQILHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASRKGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARATGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            complement(7919..8617)
                     /locus_tag="WGR74_001381"
     CDS             complement(7919..8617)
                     /locus_tag="WGR74_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0905788.1"
                     /translation="MKSKHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAH"
     gene            complement(8911..9018)
                     /locus_tag="WGR74_001382"
                     /pseudo
     CDS             complement(8911..9018)
                     /locus_tag="WGR74_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            9076..10614
                     /gene="murJ"
                     /locus_tag="WGR74_001383"
     CDS             9076..10614
                     /gene="murJ"
                     /locus_tag="WGR74_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ0905789.1"
                     /translation="MNMLGALVKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LSFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGMAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHAGLSLAIGLGACINAGLLFFLLRRHGIYRPGKGWAAFLAKMLL
                     SLAVMGGGLWAAQACLPFEWVRAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVEN"
     gene            10746..11702
                     /locus_tag="WGR74_001384"
     CDS             10746..11702
                     /locus_tag="WGR74_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HEZ0905790.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRSAPAYRRELLPDSTGKT
                     KTAYDFSDGISPDAPLVVLFHGLEGGSGSHYAVELMLAVRDRGWNGVVVHFRSCGGVA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGEQGENALPQAAA
                     VISAPVDAEAAGNRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAVNDPFLPPEALPRADEVSEAV
                     TLFQPTHGGHVGFVGSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            11753..12535
                     /gene="trpC"
                     /locus_tag="WGR74_001385"
     CDS             11753..12535
                     /gene="trpC"
                     /locus_tag="WGR74_001385"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ0905791.1"
                     /translation="MTDILNKILTAKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIGS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPPEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEREQLERFEAVA
                     HELGMTVLLELHDESELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMQSRGVHTFLIGETFMRADNIEAEVGKLF"
     gene            12601..14901
                     /gene="recQ"
                     /locus_tag="WGR74_001386"
     CDS             12601..14901
                     /gene="recQ"
                     /locus_tag="WGR74_001386"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ0905792.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLEFIRKEMTGQSGIVYCLSRKKVEDVARFLRENGLNAIPYHAGLGMDVREENQRRF
                     TREDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSGEVQKQIEMQKLDAMLAVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGAELSDKEWRSVIRQCISLGYLTVNIARYQALQLTEAAKKVLKGKTGVMLRPLK
                     RNKPAVRTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QNLNGLHDIYGLGEAKTERFGHGILEVCRNAAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLAATPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDEAAKRKRRLMRALIQWCGETAKHEQSEPYRILSKAALRAIAAKQPKGLAELA
                     AVYGVGEEKTARYGAAVLAVLGQNAV"
BASE COUNT         3149 a         4218 c         4178 g         3405 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataacgc aagcaccgca aagacgcgcc aaccctcccc
       61 caaccttttt tcagacggca ttttcggtaa tctgctaaaa tcgcccgctt gagtttccac
      121 agaaaaatcc gaaaaatgaa tattttttac gaagagtccg gccaattcaa aatcgccgcc
      181 atcatccaaa aaaacgatgc cacctaccaa gtcgataccc cacacggcaa acgcaccaaa
      241 gtgaaggcga acaacgtctt tgccgagttt gacggcgata tggcggcgtt tttggaaaac
      301 gcgcaggtgc aggcggcgga catcgacacc gatttattgt gggaagtatg cggcggagag
      361 gaatttgccg ccgaagccat cgccgaagaa tattacggcc atacgccgac caaaaccgag
      421 ctggcggcga ctttgattgc gctttacgcc gcaccgatgt atttctacaa aaaagccaaa
      481 ggcgtgttca aagccgcgcc cgaagaaacc ttaaaacaag cgcttgccgc catcgaacgc
      541 aaaaaacaac aggacgcgca aatcgacgct tgggcggaaa ccttgaaacg cggcgagatg
      601 ccgtctgaaa tcgcggcgga tttgaaaacc atcttacacg cgcccgacaa gcagtcgctg
      661 acctacaaag cctttaccaa agccgccgac gcgctgaaaa cctccgccta cgaactggcg
      721 aaaaaaacgg gcggcattac gtccattccc caatacctgc aagacgggtt tgaaatcaaa
      781 tacttcccca aaggaacagg ttttcccgac cttccgcttc ctgaaatgcc cgacctgccc
      841 aaagccgacg ttaccgcctt ttccattgac gacgaatcga ccaccgaagt ggacgacgct
      901 ttaagcctga ccgacttggg caacggcacg aagcgcgtcg gcatccacat tgccgcgccg
      961 tcgcttgccg ttaaaccggg cgacaaaatg gaacaagtca ttatgcagcg gttgagtacg
     1021 gtttattttc ccaacggcaa aatcactatg ctgcctgaaa actggatagc cgcgttcagc
     1081 cttgatgcag gcgcacaccg ccctgccgtc agcatttatt tcgatgtgga cggcgagttc
     1141 aacgtcggcg cgccgacctg caaaatcgaa gcggtcaaca tctccgaaaa cctgcgtatc
     1201 caagccatcg aaccgcattt caacgccgaa gcgggcttgg acgaagcggg cgaaatgatg
     1261 ttcgcccacc atcaagacct gatttggttt tatcaattcg ccaccgccct gcaaaaagcg
     1321 cgcggcaaat acgaacccga ccgcgcgccg caatacgatt acagcatcga attggataaa
     1381 gaagacaacg tatccgtcgt ccgccgcgaa cgcggttcgc ccatcgatac gctggtcagc
     1441 gagatgatga ttcttgccaa cagcacttgg gcgcaaatgc tccatgacaa cgacctgccc
     1501 ggcctcttcc gcgtccaacc caccggaaaa gtacgcatga gcaccaaatc cgagccgcat
     1561 atcggcatgg gcgtgcggca ttacggctgg tttacctcgc cgctgcgccg cgccgccgac
     1621 tacatcaacc aaaagcagct gatcagcctg atagacgaca ctgccgagcc gctgtatcaa
     1681 aacagcgatg ccgagctttt cgccgcactg cgcgactttg atgccgccta taccgcctac
     1741 gccgacttcc aacgccaaat ggaagcctac tggagcctgg tttacctgca acagcaaggc
     1801 acgagcgagc tgacggcgac gattctgaaa gaagacctcg tccgcatcga aggtctgccg
     1861 ctagtaacac gcgcgaccgg tattccgttt gacgcgctgc ccaaatcgca ggcattgttc
     1921 aaaatcaccg aattagatgc cgagaagcag tttgtctcgc tcaactacat caaggcagtc
     1981 gcacccgcgg gtacaacggc aggcaatgcc gtctgaagcc cgatacggca aactccggca
     2041 aaaacagaaa cctgaatctc atcattccca cagtcgggaa tccggttttt tgggtttccg
     2101 ttgtttttcc gtttcaatga acttccaagc cgccgttccc gataaatacc cgcaatctaa
     2161 aatcccgtca ttcccacgca gatgggaatc taggtttatc cgcacagaaa ctgatatgcc
     2221 gtcattcccg cgaaagcgga aatccagatt cttcggtaca gaaacttatc ggataaaacg
     2281 gtttctttag attctacgtc ctagattccc gcctgcgcgg gaatgacggc ataggggttt
     2341 ccgttttccc gataaattac cacaacccaa aatcccgtca ttcccgtaaa agcgggaatc
     2401 cggttcgttc ggtttcgttt tttttgagtt tcgggcaact tctgaatcgt cattcccacg
     2461 caggtgggaa tctaggtctg tccgcacggg aacttatgcg ccgtcattcc cgcgaaagcg
     2521 ggaatccaga ccgttcggct tcagtttttt ggtttcgggc aacttctgca tcgtcattcc
     2581 cacacaggtg ggaatctagg tctgtccgca cgggaactta tgcgccgtca ttcccgcgaa
     2641 agcgggaatc cggttcgttc ggtttcgttt tttttgagtt tcgggcaact tctgaatcgt
     2701 cattcccaca caggtgggaa tctaggtctg tccgcacggg aacttatgcg ccgtcattcc
     2761 cgcgaaagcg ggaatccaga cccttcggca cagaaactta tcggataaaa cggttgcttt
     2821 agattctacg tcctagattc ccgctttcgc gggaatgacg gcatagaggt ttctgttttc
     2881 ccgataaatt cctgttactt ttcgttgcta gattcccgcc tacgcgggaa tgacgatgga
     2941 aagattgttg ttgcttcgga taaatttctg cagttttaaa taaccggatt cccgcctgct
     3001 cgggaatgac ggcataggtt tttttgtttt tccgataaat taccacaacc caaaatcccg
     3061 tcattcccgc gacggcggga atccagtcct ttaaactcca gccattcccg ataaattcct
     3121 gttacttttc gtttctagat tcccgctttc gcgggaatga tgaatgacgg tgtaaaagta
     3181 actcgaaatc caaaaagcca taccgcccgg atttttgccg atcggtatgg ctttttgctt
     3241 caaaccgcct tagcggatgt cgacatacgc gccggaatgc aggtcacgca acaggttgac
     3301 ggttgcctgt tcggcttttt gttggaagat gtattgccgc acggaattgc ggatacgttc
     3361 ctgaggtgtg ccggcatcgc gcacttcgtt caatttgatg atatgccagc cgaattgggt
     3421 gcggacgggc gcgccgacct gtccgggttt gagcgcgtgg acggcttctt caaaggcggg
     3481 aaccatcacg ccgtcggcaa accagcccaa atctccgccg ttgcccgcgc tcgcgtcttg
     3541 cgaatattgg cgcgctaggc tggaaaagtc tgtgccgctg cgggcctctc cgtagatttt
     3601 gcggatggtg ctttccgcgc cgacggcggc gttttcgctg tcggctttaa tcaggatgtg
     3661 ttgggcgcgg tattggcgca acggtgcgcc ttcgggcagg gtgatgcctt gtttttgcgc
     3721 ctgctcgagg aaggcatcga tttcagcttc gctcacgcgg ctgttctgca tcactgcctg
     3781 ctggcggact ttttcggcaa tgatgttgtc ggcaaaatcg cggcgttggg cggggctgag
     3841 gtttttgagg gcggaatttt ttgcgacgac ggcatcgatt tccgcttcgc ttgcttgaat
     3901 gttgcggcgt ttgcccgcct gtacaatcag ggattggttg acaagctgca tcagcacctg
     3961 tcgggacagc tcggattcgc ttatctgcgc gtctttgggc aggttggctt tggcttcggc
     4021 aacggcttcg gcaagccggc ggcgcgtgat gacttcgttg tcggcaacgg cggcaatgcc
     4081 gtctgaaaag cggataccgc cctgctgttg tgcgggtgcg gcttcttttt gcgccgtggc
     4141 ggcaactttg gcaactttgg cagctttggc agctttggca gctttggcgg atgcggtttt
     4201 tgccttttgc ggtgcggcgt ggacatcggc tgctgccagc aatgcggcgg caatcatcag
     4261 ggctttgatt ttcatcattc tttccttatc gtttgaatcc tgcctttgat ggcgggtttg
     4321 gggttgtcgg tggaatgccg tcttgtgcgg gaacggtgct cctgccgggt ttccgacggt
     4381 cagggccgtt tgttgcgtcc ggcggaaaga gagtgggcgg ggatatagcc gggaacggcg
     4441 acatccatcc tgtctgcggg gtttctgccg acactgctga ggtctttcaa ctgaagtgag
     4501 aaaaagacag cgtttttgta ggtgttttcg ccggtaacgt agcgttgggc gtacacgccc
     4561 gcgccccagc agccgcaact gcttttgtat tccgcacccg ccagcacctc tatcggtttt
     4621 ttggcttcaa aaccgtagtt gtaacggacg acggccgaca ggttgcgcgt cagcggccat
     4681 tgtgcggaca ggtcgagctg gctgagtttg tcgtaaaaat aggaaccgtc ggacttcagg
     4741 tagatttttt cgttgcgccc gtatttgtag cgggcgttca gcactttgcc ctgtgcggga
     4801 cggtagcttg caccgacggc gtagttctcg gcgcgtttgt cgttttggtt gtagtggatg
     4861 ctgctgtcga ggatgaagcg gctgccgatg ccgctggagg cgaatgccac ccagtcggaa
     4921 cggctgcgcg gttttttgcc gacactgccg tcaagcatga ctgcgtcgtt tttgaagtag
     4981 aatttctgcc cgatgccggc gcggaaacgc tcttcccccg tcgcgccgtc caaaatacgg
     5041 ctttgcacgg cggcggaaag gctgtttgcg gtgttaatcc tgtcgttgcc gtaatagagg
     5101 ttttcacgaa aaagctgccc gtagccgaag ctgctttccg acgaatcgaa attgggcagg
     5161 tcgttttggg atttggcagg aatatagttg tagaacaggc gcggctcgag ggtttgcagg
     5221 actccgccgc cgaacatccg cgtattgcgt tcgaaggtca tgccgctgtc gatgttgacg
     5281 atgggcagag tgcggctgac gcgtcgggct tcttggctgc cgaagcggtt gaggctgtaa
     5341 taggtggcgt gcagtccgag tttgggacgg acgtaacccc agctgttgct gaaatcccat
     5401 ttgatgtcgg gatagacgac gaggcggctg ccgtcttggc ggctgtcgtg gctgaagcgg
     5461 gtaaattggg cggacacgcc gatttgcgcc ctgccggtgt ttttgcgcca atcggcggaa
     5521 aggcgcggca tcagggcata cggtttgtct ttgtagccgc tttggtttgc cagcgtctgg
     5581 tatttcagaa ccgaaaggcc ggcattcagg ctgccgcccg ccgccctgcc gccataatcc
     5641 agccatacac ggcggttgag gttgacgttg ccggcgattt ctttgttgcc gtaaaagtcg
     5701 cggtagtagc cgctgtcgga gacttggttg aaatcgacac ccgcctgaag cgtgtcggaa
     5761 atgtcgtgcc ggtgctgcca tttcgcctga tagcggttat tcctgccgct tttcttgtcg
     5821 tgcggcagcc aggtcaggtc ggactggccg gcataatccg gccgcaggta gcgtacctgc
     5881 ccgtcaaaga ccgcgccgcg ttcgccgatc acgccgggcg cgaacgtggc atcgagattg
     5941 ggggcaaggt tgaaataata gggaacggaa agggaaacgc cgtccgaacc ggcggacagt
     6001 gagggaacga gcaggccgct tttgcggttg ccgtcaagcg ggaagtccgc ccaaggggtg
     6061 tagaaaatgg gaacgccgcc gaacacgaag gcggcgtgtt tggcaacgcc tatgcctttt
     6121 tcccgatcgg cttcgacgga agcggccttg acataccagc cggcatcgcc ggcggaacag
     6181 gtgttgaatt gggtttccgt cagtttgtaa tgcccttcgc ccaacatttc ggcggtgcgg
     6241 ctgacgcttt gcagccgccg tccgccgtgt tcggtttcca tacggacgtt gtgcgcttcc
     6301 ccggtctgct gctcgagatt gtaggtcagg gtttcgcccc gaatcagcgt accgtcctgt
     6361 tggagggcga accggtcgcc tgcggtaacg gtgtcgcccg actggtcgta atccgcccaa
     6421 tcggcattga gggtcgtccg attgcgttcg acgacgacgt tgccttcggc acgcacctgc
     6481 acctgcgact gtccttccat cctgtcggca acaatgcgcg tatagtcttc ggggatggat
     6541 gcttcgccgc tgccttggac ggcggcttcg gttctctcgg ggctgccgct ttcgttactg
     6601 caaaacaggc aggtcgaacc gaggctcagg ctggtaggct gtatgggttc ggacacgctc
     6661 cgaacgcttc ctccggcggt cggattgtcc gtttcctccg ccgcaacggc atcggcggcg
     6721 gcgcaatgcg tgccgaaaca gaagcccaat gccagcacca gtggtttgag tgaaaataaa
     6781 cgagccaaaa tcgcccctca agtcggtttg ccagttagaa tagcgtttat tgtaacctga
     6841 aatgctttag tactgttatg caacggcaaa ccgaactgaa aaattggctt cagaccatct
     6901 atcccgaacg ggacttcgat ctgtccttcg cggcggcgga tgctgatttc cgccgctatt
     6961 tccgtgcaac gttttcagac ggcggcagcg tcgtctgtat ggacgcgccg cccgacaaga
     7021 tgagtgtcgc gccttattta aaagtgcaga aactgtttga catggtcaat gtgccgcaga
     7081 tattgcacgc ggacacggat ttggggtttg tggtattgaa cgacttgggc aatacgacgt
     7141 ttttgaccgc gatgcttcag gaacagggcg aaacggcgca caaagccctg cttttggagg
     7201 ccatcggcga gttggtcgaa ttgcagaagg cgagccgtaa aggggttttg cccgaatatg
     7261 accgtgaaac gatgttgcgc gaaatcaacc tgttcccgga atggtttgtc gcaaaagaat
     7321 tggggcgcga attaacattc aaacaacgcc aactttggca gcaaaccgtc gatacgctgc
     7381 tgccgcccct gttggcgcag cccaaagtct atgtgcaccg cgactttatc gtccgcaacc
     7441 tgatgctgac gcgcggcaga ccgggcgttt tagacttcca agacgcgctt tacggcccga
     7501 tttcctacga tttggtgtcg ctgttgcgcg atgcctttat cgaatgggaa gaagaatttg
     7561 tcttggactt ggttatccgc tactgggaaa aggcgcgggc taccggcttg cccgtccccg
     7621 aggcgtttga cgagttttac cgctggttcg aatggatggg cgtgcagcgg cacttgaagg
     7681 ttgcaggcat cttcgcacgc ctgtactacc gcgacggcaa agacaaatac cgtcccgaaa
     7741 tcccgcgttt cctcaattac ctgcgccgcg tatcgcgccg ttatgccgaa ctcgccccgc
     7801 tctacgcgct cttggtcgaa ctggtcggcg atgaagaact ggaaacgggc tttacgtttt
     7861 aaacccaatc aaaatgccgt ctgaaaacca agtttcagac ggcattttcc aaacgggctt
     7921 agtgcgcggc tttttgttct tcgcgtactt tgtccgccaa aaggtcgatg gtgttcatac
     7981 cggactccca gtcggcaaat tcgactttat atttgccgcc gacgataacc gtgggcgtac
     8041 cgtcgatttg gaaggtttcg gtcagctcct gcattttgtc ggcgcgcgcc tggctttcgg
     8101 gagattcgta agcggcaagg acttttttgc cgtcaaaggc ggtttgttcg cccagccatt
     8161 ttttgaggac ttccggctct tgcagcttga ttttttggtt gaccatcgca tcgaaaatat
     8221 ggctgttcgc cacatctttg ctgtcggcgg cagccatatc gacggcggcg gcgaggcgtg
     8281 cgagcgtcag catttctttc tgccagacga cgtgttcggt acgcaggtac atatcgtctt
     8341 taaaagactt ggcgtgtttg cttaaaacag gttcgaggtg ggcgcagtgc ggacagaaat
     8401 agccgaaaaa ctcaaggact tcgactttgc ctgcctgctg ttggggaatc gggttggcaa
     8461 ggacagtata gttttgccct tcgaccagcc ctgccggggc ggcggctgcc gaagcggcag
     8521 gcgcgctgtc ggcggggacg ctggtttgga ctttgctgtc gcacgcggca agtgcgaaca
     8581 gggcggcaac gccgagggcg aggtgtttgg atttcatacg gctctccgtg atgttggaaa
     8641 cagatgttgg aaatcaatcg gaatccggct attttattgc atttccgcgt gttgatacag
     8701 tttgcctccg gaaaaggacg ttttcgtttg gggaaaccgc ttcggacggc atcaaacccg
     8761 atgccgtctg aagcggtttc tgtcgtacaa tacgcgccgt cgccccagac ggatacggct
     8821 gttggggaac taatgatgtt ccaacacaca ggacggcaca taaagcaccg ccctatgcgt
     8881 tgccctggtt cggaagggtt acaccccttt caaatatagt ggattaaatt taaaccagta
     8941 cagcgttgcc tcgccttgcc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg
     9001 atttaaattt aatccactat caaagtctga tcctgccgcc ctaaagggcg gggtttcaac
     9061 cgaaaaggaa atacgatgaa tatgctggga gctttggtaa aagtcggcag cctgacgatg
     9121 gtgtcgcgcg ttttgggatt tgtgcgcgat acggtcattg cgcgcgcatt cggcgcaggc
     9181 atggcgacgg atgcgttctt tgtcgcgttc aaactgccca acctgcttcg ccgcgtgttt
     9241 gcggaggggg cgtttgccca agcgtttgtg ccgattttgg cggaatataa ggaaacgcgt
     9301 tctaaagagg cgacggaggc ttttatccgc catgtggcgg ggatgctgtc gtttgtactg
     9361 gtcatcgtta ccgcgctggg catacttgcc gcgccttggg tgatttatgt ttccgcaccc
     9421 ggttttgcca aagatgccga caaatttcag ctctctatcg atttgctgcg gattacgttt
     9481 ccttatatct tattgatttc actttcctct tttgtcggct cggtactcaa ttcctatcat
     9541 aaattcagca ttcctgcgtt tacgcccacg ttcctgaacg tgtcgtttat cgtattcgcg
     9601 ctgtttttcg tgccgtattt cgatcctccc gttaccgcgc tggcttgggc ggtttttgtc
     9661 ggcggcattt tgcaactcgg cttccaactg ccctggctgg cgaaactggg ttttttgaaa
     9721 ctgcccaaac tgagtttcaa agatgcggcg gtcaaccgcg tgatgaaaca gatggcgcct
     9781 gcgattttgg gcgtgagcgt ggcgcagatt tctttggtga tcaacacgat tttcgcgtct
     9841 tatctgcaat cgggcagcgt ttcatggatg tattacgccg accgcatgat ggaactgccc
     9901 ggcggcgtgc tgggggcggc actcggtacg attttgctgc cgactttgtc caaacactcg
     9961 gcaaaccaag atacggaaca gttttccgcc ctgctcgact ggggtttgcg cctgtgcatg
    10021 ctgctgacgc tgccggcggc ggtcggaatg gcggtgttgt cgttcccgct ggtggcaacc
    10081 ttgtttatgt accgtgaatt cacgctgttt gacgcgcaga tgacgcaaca cgcgctgatt
    10141 gcctattctt tcggtttaat cggtttgatc atgattaaag tgttggcgcc cggcttctat
    10201 gcgcggcaaa acatcaaaac gcccgtcaaa atcgccatct tcacgctcat ctgcacgcag
    10261 ttgatgaacc ttgcctttat cggcccgttg aaacacgccg ggctttcgct cgccatcggt
    10321 ttgggcgcgt gcatcaatgc cggattgttg ttcttcctgt tacgcagaca cggcatttac
    10381 cggcccggca agggttgggc ggcgttcttg gcgaaaatgc tgctctcgct cgccgtgatg
    10441 ggcggcggac tgtgggcggc gcaggcttgc ctgccgtttg aatgggtacg cgccggcgga
    10501 atgcggaaag cggggcagct ctgcatcctg attgccgtcg gcggcggact gtatttcgca
    10561 tcactggcgg ctttgggctt ccgtccgcgc catttcaaac gcgtggaaaa ctgaccgatg
    10621 ccgatatcct ttttcggcaa acgcttttcc acgcctatat tcgggcgcgc atcccccacg
    10681 tccgcaccag ccggacgggc tgccgcatca tcaatgccgt ctgaaaacaa gaaaaccgat
    10741 accttatgat tttgacaccg ccggacacac cctttttcct ccgcaacggc aatgccgaca
    10801 cgattgccgc caaattcctg caacgctccg cacctgcata ccgccgcgag ctgcttcccg
    10861 acagcacggg taaaaccaaa accgcctacg acttttcaga cggcatttcg cccgatgcgc
    10921 cgctggtcgt gctgtttcac ggtttggagg gcggcagtgg cagccattac gcggtcgaac
    10981 tgatgctcgc ggtacgcgat cggggttgga acggcgtagt cgtccatttc cgcagctgcg
    11041 gcggcgtagc gaacaccgcc ccggtgttct accacttggg cgataccgcc gaaattgcct
    11101 ttactttgga cacgctcgcc gcgcgttacc gtgaaatata cgccgtcggc gtatcgctgg
    11161 gcggcaacgc gctggcaaaa tatttgggcg aacagggcga aaacgcgctg ccgcaagccg
    11221 ccgccgtcat ctccgcaccc gtcgatgcag aggcggcagg caaccgcttc gacagcggca
    11281 tcacacggct gctctacacg cgctacttcc tccgcacact gatacccaaa gcacggtcgc
    11341 tccaaggttt tcagacggca tttgccgcag ggtgcaaaac actgggcgag tttgacgacc
    11401 gtttcaccgc accgctgcac ggctttgccg atcggcacga ctactaccgc caaacttcct
    11461 gcaaaccgct gctcaaacac gttgccaaac cgctgctcct gctcaatgcc gtcaacgacc
    11521 ccttcctgcc gcccgaagcg ctgccccgcg cagacgaagt gtccgaagcc gttaccctgt
    11581 tccagccgac acacggtggt catgtcggct ttgtcggcag caccggcggc aggctgcacc
    11641 tgcaatggtt gccgcagacc gtcctgtcct atttcgacag cttccgcaca aacaggcgtt
    11701 aacggtttga tgctaatatt ccccctttcc ccagacgaat acggaacacg acatgaccga
    11761 catcctcaat aaaatcctta ccgccaaggc acaggaagtt gccgcccaaa aagccgccgt
    11821 caacgccgaa cacatccgcg cacttgccgc agaagccgcg cccgtccgca gctttatcgg
    11881 ttcgatacgc ggcaaacacc gcctcaacct gcccgccgtc attgccgaaa tcaaaaaggc
    11941 aagcccgagc aaagggttaa tccgtccgga cttccgaccg cccgagattg cacgcgccta
    12001 tgaaaacgcc ggggcggcgt gtttgtccgt actgaccgac gaaccctatt tccaaggttc
    12061 gcccgaatac ctcaaacagg cgcgcgaagc cgtatcgctg cccgtgctgc gcaaagactt
    12121 catcatcgac gaataccagg tttatcaggc gcgcgcctgg ggggcggatg ccgtcctgct
    12181 gattgccgcc gcactggaac gggaacaatt ggaacgcttt gaagcggtgg cgcacgaatt
    12241 gggcatgacc gtcctgctcg agctgcacga tgaaagcgaa ttggaaaaat gccgcaacct
    12301 gaccacgccg ttgtggggtg taaacaaccg caacctgagg acttttgaag tctccctcga
    12361 ccaaaccctg tcgctgctgc ccgcgctgga aggcaaaacc gtcgttaccg aaagcggcat
    12421 tacaggcaag gcggatgtgg aatttatgca atcgcgcggc gtgcatacct tcctgatcgg
    12481 cgaaacgttt atgcgtgccg acaatattga ggcggaagta ggcaaactct tctaaatccc
    12541 gatttcagac ggcattccct ttctttaacc gacacaccct ttaccggaca cggaaccatc
    12601 atgcaccgac cgaccgccaa acagattctg cacgaagtat ttggttatcc cgaatttcgc
    12661 ggcaggcagg aggctgtcat caatactttg gcaggcggcg ggagtttgac ggtgctgatg
    12721 ccgacgggcg ggggcaagtc tttgtgttac cagattcccg cgctgatgcg cgaaggcgtg
    12781 gcggttgtcg tatcgccgct gattgcgctg atgaacgacc aagtggccag cctgcatgtg
    12841 gccgggattg aagcagcggc agtcaacagt ggtacatcgg cagatgaggc gcgcgagatt
    12901 gccgacaagc ttgcccaagg ccgtctgaag ctgctttatg tcgcaccgga acgcctagtt
    12961 accgaccgct ttttacgttt tctcgaccaa caaaccgtca gcctgtttgc cattgatgag
    13021 gcgcattgcg tcagccagtg gggacacgat ttccgccccg aataccaaca gctcggcatg
    13081 cttgccgaac gctatccgaa cgtcccgcgc atcgccctga ccgctaccgc cgatgccgcc
    13141 acgcgcgccg acatcaagca ttatctgcac ttggacgacg cgcccgaatt tgtctccagc
    13201 tttgaccgcc cgaatattta ttatcaggtt atcgaaaaaa acaacggcaa aaaacaactg
    13261 cttgaattca tccgcaaaga aatgacgggg caaagcggca ttgtgtattg cttaagccgc
    13321 aaaaaggttg aagatgtggc gcggtttttg cgtgaaaacg gattaaacgc gattccgtat
    13381 cacgccggtt tgggtatgga cgtgcgtgag gaaaaccaac gccgctttac gcgtgaagac
    13441 aatattatcg tggtggcgac cgtggcgttc ggtatgggca tagacaaacc cgacgtgcgc
    13501 tttgtcgccc atctcgatat gccccagagt gtcgaacatt tctatcagga atcggggcgc
    13561 gccggccggg acgggctgcc tgccgcgagc tggctgtgtt acggcttgaa cgattgggtg
    13621 ttgctgcgcg aacggattgc cgaaggcaac agcggcgaag tgcaaaagca aatcgaaatg
    13681 caaaaactcg atgccatgct tgccgtctgc gaaaccgccg cctgccgccg cgtgctgctg
    13741 ctcaaacatt tcggcgaagc atccgaaccc tgcggccatt gcgacaactg cctgcatccg
    13801 cccgtacggt ttgacggcac ggtgttggtg caaaaattac tcagctgcgt gtaccgcgcc
    13861 ggacaacgtt ttgccgccgg ttacatcacc aaccttttgc gcggtaaaag cgacgattgg
    13921 atacgcggca accggcacga acaactgtcc acattcggca tcggtgcaga attgtccgac
    13981 aaagaatggc gcagcgtcat ccgccagtgt atcagcctcg gctacctcac cgtcaacatt
    14041 gcccgatatc aggcattgca actgaccgaa gccgccaaaa aagtcctcaa aggcaaaacc
    14101 ggagtgatgc tgcgtccgct caaacgcaac aagcccgccg tccgcaccct caaagacaac
    14161 tggctgcgta ccgaacgcga agaacgcctg tggcaggcat tgcgcgtttg gcgtatgaaa
    14221 caggcagaag ccgaaggcat ccccgcctat atgattttcg gcgacaaaac cctgcgcgac
    14281 cttgtcgaaa aaatgccgca aaacctcaac gggctgcacg acatctacgg cttgggcgaa
    14341 gccaaaaccg aacgtttcgg acacggcata ctcgaagtct gccgcaacgc cgccggcttc
    14401 agtcgcgacg cggtcatccg tccgcaaacc gaacgcgaac aacaactgcg tcaaaaactc
    14461 gaggcctggc ggtatgaaca ggcaagggcg gaaaactgcg ccctgcatgc cgtcctctcc
    14521 gacgaaagcc ttgccgatat gcttgccgcc acgcccgaaa ccgaaaccga cctcgaaggc
    14581 gtacacggct tgggcagcgt acgcgccgcc aaatacggac gggacatcct cgccgtctgc
    14641 cgtccgtttt cagacggcat cgatgaagcc gccaaacgca aacgccgcct gatgcgcgcc
    14701 ctgatccaat ggtgcggcga aacggcaaaa cacgaacagt ccgaacccta ccgcattctc
    14761 agcaaagccg ccctgcgcgc cattgccgcc aaacagccga agggtttggc ggagcttgcc
    14821 gccgtatacg gcgtgggcga agaaaaaacc gcacgttacg gtgcggcggt gttggcggtg
    14881 ttgggacaaa atgccgtctg aaaaccgtta ccaagtttca gacggcattg cctctattta
    14941 aaaattcctg
//
LOCUS       DAWFLT010000048        14839 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWFLT010000048 DAWFLT010000000
VERSION     DAWFLT010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14839
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.048"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(148..843)
                     /locus_tag="WGR74_001387"
     CDS             complement(148..843)
                     /locus_tag="WGR74_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0905793.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSAQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(1014..1673)
                     /locus_tag="WGR74_001388"
     CDS             complement(1014..1673)
                     /locus_tag="WGR74_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEZ0905794.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRCNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            complement(1871..3938)
                     /locus_tag="WGR74_001389"
                     /pseudo
     CDS             complement(1871..3938)
                     /locus_tag="WGR74_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(4124..4729)
                     /locus_tag="WGR74_001390"
     CDS             complement(4124..4729)
                     /locus_tag="WGR74_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0905795.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAAVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLATVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            complement(4778..5176)
                     /locus_tag="WGR74_001391"
     CDS             complement(4778..5176)
                     /locus_tag="WGR74_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0905796.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            5284..6195
                     /locus_tag="WGR74_001392"
     CDS             5284..6195
                     /locus_tag="WGR74_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0905797.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            6185..7486
                     /locus_tag="WGR74_001393"
     CDS             6185..7486
                     /locus_tag="WGR74_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ0905798.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCTAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAAAHKTDPDGT
                     FVRRHIPELAHLSADTVHTPWRFACSVDTHGYPAHPVAGIP"
     gene            7549..7845
                     /locus_tag="WGR74_001394"
     CDS             7549..7845
                     /locus_tag="WGR74_001394"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905799.1"
                     /translation="MTQYLPFFSLSIIRYAQITATQAATVPIVGKKCCQSVIGGTGQR
                     PHSISAPPNNRYSSISAIQSNNGYFKRCNQKTNHVRTIYLPPFRQRYCTASVQC"
     gene            7879..10029
                     /gene="dinG"
                     /locus_tag="WGR74_001395"
     CDS             7879..10029
                     /gene="dinG"
                     /locus_tag="WGR74_001395"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ0905800.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LTREEGEEAPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQNNAQQNLLGFEAPEVLWD
                     SKPKPEELNLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKAACPNRA
                     ECPFYLARDMLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAASSLLDSLHEWQFHLAE
                     EPSLSLGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVIGLNDALS
                     AARREKEQDSALIDRLTGEFGLFISRIEQISAVWDLLSTVPIEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAIIEWLPKLISPGEAIGTLVLFSSRK
                     QMQDVALQLPDDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRVKTQRYGQQLLAGLPPFKRIG"
     gene            10065..10679
                     /locus_tag="WGR74_001396"
     CDS             10065..10679
                     /locus_tag="WGR74_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ0905801.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIEDIAAAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALELDDNLVENLERQLGF"
     gene            complement(10774..12642)
                     /locus_tag="WGR74_001397"
     CDS             complement(10774..12642)
                     /locus_tag="WGR74_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0905802.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVMIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFPARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLIKDIPNV
                     HWVDAQKYLPKNTVEIYGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRDGALQ"
     gene            complement(12723..14093)
                     /gene="purB"
                     /locus_tag="WGR74_001398"
     CDS             complement(12723..14093)
                     /gene="purB"
                     /locus_tag="WGR74_001398"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ0905803.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKSLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLAEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIGLLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            complement(14165..14797)
                     /locus_tag="WGR74_001399"
     CDS             complement(14165..14797)
                     /locus_tag="WGR74_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ0905804.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFSCRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAASLFAISDDEDEIVAYLSEHGLQTA"
BASE COUNT         3542 a         4105 c         3822 g         3370 t
ORIGIN      
        1 caacgccgta ctggtttttg ttaatccgct atatattctg atttaaaacc ataaggcttt
       61 aagcaatcat ctcttctata aagcctaaat acaaaaggcc gtctgaaatc ctattttcag
      121 atggccttta ctcattcaat cctcaactta ttgcggcttt ttctgctctt cgcgtacttt
      181 atccaccaac tggtcaatcg tggtcatacc ggactgccag tctttaaatt caacttggta
      241 tttgccgccg acaatcacag tcggtgtgcc gctgatttgg aatttattgg tcaactcttc
      301 catttgagcc gcacgcgctt ggctttcagg agcctcaaat gcagccaata cttttttgcc
      361 gtcaaacgct gtttgctcgg acagccattt tttcagggta tcggtatcgg ccagattgat
      421 tttttgatta accatcgcat cgaaaatatg gctgttggct ttatctgatt caccggccat
      481 ttccactgcg gccgccaaac gtgccaaagg tttcatttca tcaccccaca cgacatgctc
      541 ccggcgcata taggtatcgt ctttaaacgt tttgatgtgc tcgctcaaga ccggctcaag
      601 atgggcgcaa tgcgggcaga agtagccgaa aaattccaat acttcgattt taccggcctg
      661 ctgttgcgga ataggcgtag acaatacagt gtagttcaca ccttcgttca actcagcagg
      721 ggctgccgga gcagatgagc tgctttgggc gctgtctgcc ggaacactgg tttcagcctg
      781 tttgctacaa gcggccaatg ccaacagggt caatgaagtc aaagctaagg ttttcagttt
      841 cataggtatc tcttgtgtgt cagattaatg tgcggatttt atggcatttt attgaaggcg
      901 tgttcgattt gagtgaaaaa atgtgtttag ttttatttca gcctttataa aggccgtctg
      961 aaatatggct tagataatta gccttatttt cagacggcct ttgttctagt aatttacggt
     1021 ttcggacatt ttgtcttcaa tccgtcgatg tgttccatta agtcaggtgg gacatgattc
     1081 cacataggat caagaataaa atcgatacct tcccgtctgg ctaatttggc tgctgggaca
     1141 aaatcggaat caccagaaaa caagatgatt ttatcggctt gtttttttaa ggtaatagat
     1201 gaaatatcca agcctatacg catatctaca cctttttgtt taacatcgag cacgacatcg
     1261 ttttccgtaa tttgagttga cgggtttttc agaagatttt tcatgacttg gggttttaat
     1321 atccatgctg tattgtccga cagcttgcct aaacgtaacg ctaatttacg cttcttcttc
     1381 aattcggtat gcagattgca acgaaatatg gcttccggag tctttgaaaa atctatggca
     1441 cgatgagata cgggattgtg cattttcttt tcaaacggca cgcagtcgta aaagaaaatt
     1501 ctatataaat catccatttg atctgtgccg ttgattttat ctttcagatg cagcatcgaa
     1561 tatcggaaag ccaaatcagc aattcttttt gcatcgtgtg gattttgaga caggcctaat
     1621 gaccgtaccc gcttaataaa aaatgcaccg tcaatcaaaa tggcggtttt catattgctt
     1681 cccctatatt tgtcaaagat ataaaaaagc ccttgggatc ggcatacccg ctatgattta
     1741 atgggtaatg cgtactgcca agggcactat ttctttttgg attatactga tgtagataaa
     1801 gcctgtcaaa aagttcgtat gctgaattta cggattatca ggattttcag acggcctttg
     1861 cctgatttca tcagaaacgg taatttaccc gggcagtata gccgcgcgga ttacccggca
     1921 tagagtccga acgccaatat ttttgattga acagattggc tgcggcaaag gtaacgttaa
     1981 catttttatg gttccagcca agcatggcat caactcgggc aaagcctgga agcgtagtca
     2041 cttctttatt tcttgagtcg taaccgtagc gtttgcctgt accggttacg ccgatttcgc
     2101 cgtagaggtt ttcggtcggg gtataacgga aaaacaggtt gccggtaacg ttgctggtgt
     2161 tattcaaatg gatgcccact cggtcgggat tttctttgtc ttcaacgact ttcgcctgca
     2221 tcacgcccaa cgaaccgcgc agatagagtt tttttgggga tgatttgccc gatggcggac
     2281 aattccacgc cgcgcgaacg gtgtttgccg ctaaccgcat aaatataagg gttgtttttt
     2341 ggatcggggc ggtagcggat attgaagcgt tcgatttggt aggcagacaa cgtagtgctg
     2401 aggcggtcgt ccagccaact gcttttcacg ccggtttcgt attggcgggt gtactcgggg
     2461 tcggcgttga acacggcgga agacaacgta tcgatgctca aatagccgcc gcgtccgcca
     2521 taaggcgcga agcctttgtt atacgaggcg taaagtgtgt ggacgggatt gatgttccac
     2581 actgcgccga tgttggggct gaacgagtgt ccgctgtatt ggcggctgct gccggtgagt
     2641 ttgttttcgg aattaaaggt gtatttgtcg taacggccgc cgaggacgaa tttcaaatcg
     2701 ggcgtggcgg agaagatgtt ttgcacaaag atgccgtagg agtcggcttt gtggcggttt
     2761 tgggtcagaa taggctgcaa tctgcccgaa gccggccagc ttgcgcggtc gtaggggttg
     2821 atggaggcgg aaaaggcgct gctgaaaccc aatgtcgggt tgcggtgttc gcggctgtaa
     2881 tccatgccta cggtcaggtg gttttcaaaa cggccgatgg tgtagtcgcc gttgagcgtt
     2941 aagttggacg acagggtttt gttgtcggtc tgctgccagg cgtagttacg tttgattaag
     3001 ttgccatttt cgctgcctgc atagaaatga tcaaaatcct gcgccgccgt gcggtgggcg
     3061 agctgccatt gggcacgcca tttgtcgttg aaggcgtatt caaggtcgga acgccaaact
     3121 tgcagcttgt ctttgacaaa atcgttccgg tgggcgaacc ccatgcggta aggcagtccg
     3181 aagcggtcgt acacggactt ggtcggactg cggtcgggcg tgcgctccac attgtcgtag
     3241 gtgtattgcc ccgtccactt caagccgttg tcgagtttga cggtaatgct gggcgaaacc
     3301 atgacatttt tgctgtctat gccgctgcgg aacgaattgg cgcgcccgac ttcgccggtg
     3361 agacggatgg cgacgttttt gttcagcact tcgttaatgt ccatattcag gctgcggttt
     3421 gcccacgaac cgtaaaccgc accgatgttg cggctttgtt tgaagttggc gtatttgctg
     3481 accatgttga tgacgccgcc gccgttggtg cggccgtaaa gcacggaaga cgggcctttc
     3541 aggatttcca cgcgctcgat gttggcagta ctgcggcgca cttgtccgct ttcgcgcacg
     3601 ccgtcgcggt aaatatcgga tgcgtcggct tgaaaaccgc gcaggaaaat gctttcaccg
     3661 cgcatatcgt aggcagcgtc gatgccgaca ttgccttcga ggatggaact caaatcgttc
     3721 gtgccgtaat ttttgttttt ctggatattg cgggtaacgg cggcttcgtc gtagttgatg
     3781 tagcctttga gtacgctggt gtcggactgt ccgaccacgg aaacggtagg tagcgtggcg
     3841 gtgtaatgtt caccattgtc ctgcgtatcg gcggcagcga cagggaagga agcaataatc
     3901 agcgtgggta ataaagctaa atgaaatgat attttcattt ttatattcaa tttaacaaaa
     3961 caaccgaatt atattgcctc acggaggaaa tgagaataat ttcttttaac tatattgaac
     4021 atgatatttg taaacaaagg tctcagaatg cggaaaactc gccgcctgat actgaaaaat
     4081 gccgtctgaa cagggttcag acggcatttt tttgaccgcg aaattatgcg ccgaacactt
     4141 tcaaacgttc tgcaactggt tcaaaggtct tttcacctgc cgccccttca ataccgccga
     4201 taaccatttg tgcgcgcaac agccagtttt cgggaatatt ccacgctttg gcaatcgccg
     4261 catcgggcaa ggggttgtaa tgttgcaggt ttgcacctac gccgaccgtg gcaagtgtcg
     4321 tccagacggc atactgtacc atcgcgttcg cctggtccgc ccaaacggga aagttggcgg
     4381 cataagcagg gaactgctcc tgcaaacctt tgacgacatt ttgatcttca taaaacaaaa
     4441 tggttgccgc acccgcctta aacaggttca atttttgcgc ggtcggttca aaactgtcgg
     4501 caggcacggc ggcacgcagc gcgtcttcga caaattgcca caccttatca tgctcttcgc
     4561 caaacagcac gaccacgcgg gcagattggg aattgaacga agaaggtgtg tgcaaaacgg
     4621 cgtgttcgac gatttggaca acttcatctt tgccgacggg cagattttta tttaacgaat
     4681 aaatggaacg gcggctttcg gcagcctgtt gcagagattg acgggtcatt tcttttcctc
     4741 aaaaacattc aacaaaatac actttaggca gtccgattca ggctttacgc ttgaaaaaca
     4801 gcctgtccag cgaccatgct ccgccgcccg ccgccgcgat atagaggaat acgaagcaga
     4861 acagcactgc ggactcgccg ccgttggcaa tcgggaacaa agcatttccg gaagcgtgcg
     4921 ccataaaata ggcaaccgcc atctggccgg acaaaacaaa cgcggcaggg cgcgcaaaca
     4981 ggcccaacac cagcaaaatg ccgccgacaa tttctaaaat accggcaagc agcaacagcc
     5041 cgccgggcga accgctgccc atttcaatgg ggaaggcgaa gattttcgac gtaccgtgca
     5101 acaaaaacag gtaagcggtt acgatacgca aaacagaaag caaaaccggt tggatacggt
     5161 tgcagcaatc ggacataaaa gctcctttga tatttgtctt catttcaggc ggtagagtat
     5221 agatgtttcc ccttttcaaa tatatactgc ttttattgat acaacttttc cacacaagaa
     5281 aatatggaca ccctgttcag cctcaaggtt ttccgccaag tcgtccaaag cggcggcttc
     5341 acccgcgccg ccgacgcgct cggcatctcc accgcaatgg caagcaaaca cgtcagccac
     5401 ttggaaaaca ccgtccaagc caaactcctg caccgcaaca gccgcaacct cagcctgacc
     5461 gaagccgggg aagaatacta ccggcaatgc agttacgcgc tcgacacgct cgacgatgcc
     5521 gcgcaaaaag ccgccggggg gacggaaaaa ccgcaggggc tgctgcgcgt aacgatgcca
     5581 ctgtggtttg ccggcagcca gatatgcaac tggctggcgg aataccgcga acgttatccc
     5641 gaagtgacat tagaactgat tttggacaac cgccacgtcg atttgattgc cgaaggcgtg
     5701 gatttggcgt tgcgcgtttc ccaaaccctg tccccgtcgc tgattgcgcg cccgctggcg
     5761 gaaatcgaat ttgccctgct cgcctcgccc gatttcctga ggcgcaacgg cgtgccggaa
     5821 acgccggaag aagtggcggg gctgcccgcc gtcctgccga cctacaccaa ccagcagaaa
     5881 ctcgacctca cccgcaaatc ggacggcaaa aaataccggc ttgaactgac ccccgtcatc
     5941 cgtaccgaca acacgctgat gatgcgcgaa atgattaagg cgggcgcgtg catcggttat
     6001 cagccgcttt gggcggcgga acacgatttg cgctgcggca cgctggtgag gctgctgccc
     6061 ggatacgccg tcccgaccga ccggctgaat gccgtttatg cagacagggc attcttaagc
     6121 gcgaaagtcc gcagcttcat cgattttctg aacgaaaaaa tcgccagcag gaaaggctgc
     6181 cgaaatgccg tctgaaaccg cccgccccct ttatgcggac acgtgcgccg cacacacgct
     6241 cgtctggttc cgccaaaacc tccgcatccg cgacaacgcc gccttatgca ccgccgttgc
     6301 cgaaggtttg cccgttatcg gtatttggat tgacgatgcc gaaacagaca accctcgccg
     6361 cgccgcgttc taccgccaat ccgccgccga actcgcccaa gggcttgcag ggcgcggcat
     6421 cccgctctac acggcggcat cgcctgccga gctcgtccgg ctcgccgtcc gcctcaatat
     6481 ccgcgccgtc atcgccgacg aatcccatac ttttgccgac aaactcgccg acaacgccct
     6541 ttggcatgaa ttggacaaac acggcatcgc gttaaccttc gtcaatgacc gcgccgtttt
     6601 cggcaaaacc gacctgatac ccgacggcgg cacggcacac accgatttcg accgctaccg
     6661 cgaagtatgg ctcgaccgct tttccaagca gccccccgcc ggtccggacc tattcgcggc
     6721 ataccgccaa cccttccccg aaaacctttc cgccccgccg cctgccgccc tttcagacgg
     6781 catcttcctg ccgcaaaaca gcggcgaaac ggcggcttgg cggcagtggc ggcggtttct
     6841 cgaacaggcg gattcctact ccgttttaaa ggatttcccc tcgcgcaaac acacttcgct
     6901 gatgggcgcg tatttgagtg ccggctgcat ctcgccgcgc ctgctcgcgc gggaaagcct
     6961 cgaacgccgt ctgaacgcgt gggcggacaa catcatccgc cgcgattttt tccttcaact
     7021 tgccttgcag cacacggatg acgacccttc agacggcaat cctgaacaca ccctgcgcct
     7081 gacgctttgg cagcagggcc ggaccggcat tccgattatc gatgccgcga tgcgctgttt
     7141 gcacaaaacc ggcagcctcc accccgccct gagacgcttg agcgcggatt ttttctgcca
     7201 cgttttaaac ctcccccgcc gcgaaggcga gatatggttt gcccgacagc tgaccgattt
     7261 cgatgcagca atcaaccaag gcaactggcg gcttgccgcc tcacggcaca cctgccccga
     7321 cattgccgcc gccgcacaca agaccgaccc cgacggcacc tttgtcagac ggcacattcc
     7381 cgagcttgcc cacctgtccg ccgacaccgt ccacacgcct tggcggtttg cctgttcggt
     7441 cgatacccac ggctatcccg cccatcctgt cgccggtatt ccctaagcgg tacggcaacc
     7501 taaaacaaat gccgtctgaa cttccgtttc agacggcatt gcgtccgaat gactcaatat
     7561 cttccgtttt tttcactttc aatcatcagg tatgcccaaa tcaccgccac ccaagccgcc
     7621 accgttccca tcgtcggcaa aaaatgctgc caatccgtca tcggcggtac agggcaacgg
     7681 ccgcattcga tttccgcgcc gcccaacaac cggtacagca gcatatccgc aatacaaagc
     7741 aacaacgggt atttcaaacg ctgcaaccaa aaaaccaacc acgtccgcac gatttacctc
     7801 cccccattcc gacaacgata ttgtaccgct tccgtgcaat gttaaaataa ccccacttct
     7861 accgccgtac aaaacgccat gctcaccgat ttagaaaaaa acgccatccg cgaccattac
     7921 caaaacatcg gcaaaaacct gcccggtttc cgtccgcgtg cttcgcagcg ggaaatgatt
     7981 gcggcggttg ccaacgcttt ttcgcggacg ttgacgcgtg aagaaggcga agaagcaccc
     8041 aaacgtgagg gcgagagcat tgccgtgatc gaaggcccga ccggcgtggg caaatcgttg
     8101 gcctaccttt tggccggcgg catcatggcg caaacgcgcg gcaaacggct gattgtcagc
     8161 agcgcgacgg tggctttgca ggagcagttg gttgaccgag atttgccgtt tctggtcgaa
     8221 aaaagcggtt tggagctgac ctttgcgctt gccaaagggc gcggccgtta tctttgtccg
     8281 tacaaactct atcaactgac gcaaaacaac gcccagcaaa acctgcttgg ttttgaagcg
     8341 ccggaagtgt tgtgggacag caaacccaag cccgaagaat tgaacctgct gcgcgacatc
     8401 gccgacgaat tttccgcccg acggttcaac ggcgaccgcg acacctggcc ggaaaaaatc
     8461 gatgacgcga tttggctcaa agttaccaac gaccgccacg gctgcctgaa agccgcctgt
     8521 cccaaccgcg ccgaatgccc gttttacctg gcacgcgata tgttggaaac cgtcgatgtc
     8581 gtcgttgcca accacgatct tctgcttgcc gacatcagta tgggcggcgg cgtgattctg
     8641 cctgcgcccg aaaacagttt ctattgcata gacgaagcgc accacctgcc caaaaaagcc
     8701 ctcagccgtt ttgccgccga acattcatgg aatattgccg tttggacgct ggaaaaactg
     8761 ccgcaactga ccggcaagat tgccgcgctg accgataaag ccgaacttgc caaccttgcc
     8821 gacgaagccg cctcatcctt actcgacagc ctgcacgaat ggcagttcca tttggcggaa
     8881 gagccgtctt tgagcttggg gccgtctgaa aacgacaggc gtaccaacag cgaaccgact
     8941 tggctgtggg aagacggcaa aatccccgaa ggcctcgaaa ccaccgtttc caacaccgcc
     9001 attgccgcac gcagcctgct caaacacgtt atcgggctga acgatgcgct ttccgccgcg
     9061 cgccgcgaaa aagaacaaga cagcgcgctc atcgaccgcc tgaccggcga gttcggcctt
     9121 tttatctccc gtatcgaaca aattagtgcg gtttgggatt tgctctccac cgttcccatt
     9181 gaaggcgaag agccgttggc gaaatggata acccgccgtg ccgacgataa aaacgactat
     9241 attttcaacg ccagccccat cagcagcgcg tcccaccttg ccaacagcct gtggcggcgg
     9301 gcggcaggcg cagtgttgac ctccgccacc ctgcaatcct tgggcaactt taacctgatg
     9361 ctgcgccaaa ccgggctgca atggctgcct gaaaccacca ccctcgccct caaaagcccc
     9421 tttgactttg aaaaacaggg tgaactctac atccctccca tatacgccag ccccaaagac
     9481 cccgaagccc acaccgccgc catcatcgag tggctgccca agctcatttc gcccggcgaa
     9541 gccatcggca cgctcgtcct gttttcctcg cgcaaacaaa tgcaggatgt cgccttacaa
     9601 ctgcctgacg actatctgcc gctcttgctc gtacaaggcg aattgcccaa agccgttctc
     9661 ctgcaaaaac accaccgggc catagaagaa ggcaaagcca gcatcatctt cggactcgac
     9721 agctttgccg aaggactcga cctgcccggc accgcctgcg tgcaagtcat catcgccaaa
     9781 cttcccttcg ccatgcccga caaccccatc gaaaaaaccc aaaaccgctg gatagaacag
     9841 cgcggcggca accctttcat cgaaatcacc gtccccgaag ccggcatcaa actcatccag
     9901 gccgtcggcc gcctcatccg caccgaacaa gactacggcc gcgtaaccat cctcgacaac
     9961 cgcgtcaaaa cacagcggta cggccaacaa ttattagccg gcctgccacc gtttaaaagg
    10021 atagggtaaa cctaccgccc tgcccgcaac agaaaggaaa aatcatgaac ttcacccgac
    10081 tgctcaacca agtcttaagc acggttcaaa aaaaaggcaa caccttctcc gacagcccgc
    10141 tcaattcatt cggcggaggc gcgctggttg ccggtgtcgc ctccatgctg ctgaacggta
    10201 aaaaccgcaa aaccatcacc aaaatcggtt cgaccgccgc tttgggctac ctcgcctacc
    10261 ggggctatca gatgtggcag caaaacaaag ggcgggcaac cgtaacacaa agcgatttcc
    10321 aacctgccgg agaaaccgaa gaaacataca gccgcaccgt attgcgcacc atgatagccg
    10381 ccgccgcttc agacggcatg atagacgaag ccgaacgccg gactatcgaa caggaaagcg
    10441 gcacagaccc cgaaactgct gcatggctcg ccgccgaata ccgcctgccc gcaagcatcg
    10501 aagacatcgc cgccgccgtc ggcaacgatg aggcgttggc ggcggaggcc tatctggcgg
    10561 caagactggt atgtgccgat ttgtcgcgga aagaaaccgt cttcctcgcc cgcctgtcgc
    10621 aggctttgga actggatgac aatctggtgg aaaacttgga aaggcaattg gggttttgat
    10681 tcaaaattcc ctcccgcagg ggaaggaaaa taaaaaccgc cgccgccccg aaaggcaaac
    10741 ggcggcacaa acgatatcgg acggcaaggc aggctactgc aatgcgccgt cgcgagaaga
    10801 tttaagcagg cgttcgtgtt tgtgaaattc ccgccccata taataagaac cgaaataggt
    10861 caggtggtct tggtcgccgt aaagatagcg gccgtatatt tcgaccgtgt ttttgggcag
    10921 gtatttttgt gcgtccaccc aatgcacatt gggaatatct ttaatcaaat caaagaccgc
    10981 ctgattgctc ttgccgatgt cgcccatagc ctgaatgggg cggagatatt ggtttgcggc
    11041 aaatcttttc aatttttcct ccctcagggg cgaacggctg attgatgtgt tgtttgcaaa
    11101 aacatagacg ggtttgacgg cggctatcct tttgacggtt tccctgaatc gggctgggaa
    11161 cccgggtatt aggaaggatt gcgcttcaaa tctcggcacg ggctggccgc ccatcctcaa
    11221 atcatagaat tgggcaatga aaacggcttc ggctttttca acttcatccc ggtattttcg
    11281 acataacggg ttgtctgcca gcttctcatc tacccaaacc aaacactccg aatcgaggga
    11341 caggattttg gctttccacc cttcccggct gccgacataa tccagaaacc cccgcaggtg
    11401 tccggcgtgc gagtcgccga gggtcaggac ggtttccgga aaatgatttt ccgcagcaag
    11461 gggcgcgccg ggcaacgggc ggaggtgttc ctgtttcaat atcccccttg cgtacaggtt
    11521 gtaaccgaca agtatcaggg acggggcgag atagaggcag aaaaatgcct ttttgaaggt
    11581 catcttccgt tttctaagcg gctgttcaat caaataataa ctcaacaggg aaaatccggc
    11641 cgtcaacgcg gcaaccgccg atacggcagg cagtccgagc tgtttgtcgc ctgtaatgta
    11701 atgggcgaaa gcaataaaaa tccaatggta caggtatagg gaataagaga ttttgccgac
    11761 aaatacgatg gggcttgccg acaggatgcg ggtcggaagt gtcccgtatt gcatactccg
    11821 gataagcagt gccgtcagca ggcagggaag gagcagggtc attcccggga taaacggatt
    11881 gtgtttgtca atcacgaaca ggcaggcaag caatgcgccg aagcagagtg atgaaagcaa
    11941 ctgccgtttt ccatttgctg tttgccgtct gccgttttgc gtttgcccgt aaaccgccag
    12001 cagcgaacct gccaacagct cgggaaacct cagtgtcgaa aggtaataag tattgggttg
    12061 gttgagaata tcggtataaa acccgcttgg caaaaacgat gtggcagtca aaatcagaaa
    12121 taggatgatg ctgatgttac gcagcacccg tagcgatttt gtttttttgc agcaaaatat
    12181 cagcaaaaga ggatacagga ggtaatactg ttcctctact gccaaagacc agatatgcag
    12241 tacggggttc tcgtcggcac tcaaatcgaa atacccctgc tgaaacccca gataaatatt
    12301 ggacaagaaa accgcagaaa gctccacggt tttccgcatt tggttgaaat cttcgtaaag
    12361 gaagatttga gaggcaatca ccgaagccag cgacacggcc gcaataaaag caggataaat
    12421 ccgcttaatc ctgcgggtat aaaaatcccg gaaagaaaaa gaaccgttct gtatttcaga
    12481 aagaatgatg ccggtaatga ggaatcctga gatgacaaag aaaatgtcca cccccaggaa
    12541 tcctccgggc agccagcggt tattcaggtg gaaaatcatg acggatagca cggcgacggc
    12601 ccgcaatccg tcaatttccg gtctgtatcg gacagcttgc ataaatatcg cccctgtatg
    12661 tcccgttatc ttaaaaatgc cgtctgaacg cgcgttcaga cggcatcggt ttcagtaaac
    12721 gctcaaatcc gtttcgccaa cgcttcagcc ttgcccacat acagcgcggg ggtcagctca
    12781 agcaatttgg ctttggcttc ggcggggatt tccagcaatc cgataaagcc tttcagcact
    12841 tcgggcgtga tgccgccttt gccgcgcgtc aggtctttca gcttctcgta aggattggcc
    12901 acgccgtaac ggcgcattac ggtttgaatc ggctcggcga gcagctccca agtggcatcc
    12961 aaatcggcgg caagcgcggc ggggttaggt tcgagcttgt tcagaccgcg caggtgggcg
    13021 gcgaaaccca acacggcata gcccacgccc acgcccatat tgcgtaagac ggtgctgtcg
    13081 gtcaggtcgc gctgccaacg ggaaatcggc agtttttccg ccaaaaaacc caatacggcg
    13141 tttgccatac caaggttgcc ctcggagttt tcaaagtcga tggggttgac tttgtgcggc
    13201 atggtggaag aaccaacttc gcccgctttg actttttgtt tgaagtaccc caaagaaatg
    13261 tagccccaaa cgtcgcggtt aaagtcgatc aggatggtgt tgatgcggct gagggtttgg
    13321 aagaattcag ccatgtagtc gtgcggctcg atttggatgg tgtaggggtt gaaggtcagg
    13381 ccaaggctga tttcgacgaa gttgcggcag tgggtttccc aatcgacgtc gggataggcg
    13441 accatgtggg cgttgtagtt gccgaccgcg ccgttgattt tgccgaggaa ctcttgcgcc
    13501 tgaaggcttt taaactggcg ttgcaggcgg tacacgacat tggcggtttc tttgcccaaa
    13561 gtggtcggcg tggcgggctg gccgtgggtg cggctcatca tcgggacggc ggcaaggtcg
    13621 tgcgccatag cggtcagttt ttcgatgatt tctgccagtt tgggcagcaa aacagcctca
    13681 cgcgcttctt gcagcattaa agcgtgggac aggttgttga tgtcttcgct ggtgcaggcg
    13741 aagtggatga actcactcac ggcggcgact tccggcactt cggcaaaacg ttttttcagc
    13801 caatattcaa tggctttgac atcatgattg gtcgtggctt cgatggcttt gacggcggcc
    13861 gcgtcttcca atgaaaagtt ttcaatcacc gtgtcgattt cggcaagcgt ttcggcactg
    13921 aagggcggca cttcggcaat cttcggctcg gcggcgaggg ctttgagcca gtttaattcg
    13981 actttgacgc gcgctttcat caggccgtac tctgagaaaa tcgggcgcaa tgcttcaacg
    14041 gattgggcat aacggccgtc taaaggggaa agcgaggcga tggggttgat catttcggca
    14101 tcctgttcgg gaaagacatc aaaaattgtt aaattgtttg caattataca tcactctcag
    14161 gacgctatgc cgtctgaagc ccgtgttccg acagatacgc aacgatttcg tcttcatcgt
    14221 ccgatatggc gaacaaagag gctgcccctt cggaaatcag accgcgcgcc aaaagctgcg
    14281 cgtttatcca ctccgccaag cccgaccaaa acgcctttcc gaccaaaaca atcggacgcg
    14341 gcggcacttt gcccgtctgc accaaggtca ggatttcaaa caattcgtcc agcgtcccga
    14401 agccgcccgg catcacgaca tatgcttggg aatagcggaa aaacaccgcc ttgcgttcgg
    14461 caaaacggga aaaccgcaag gcgatgtcct gatacggatt cggtttctgc tcgtgcggca
    14521 aaacgatgtt cagccccacc gaaaccgact tcccggcaaa cgcgcccttg tttgccgcct
    14581 ccataatccc cggcccgccg cccgaaatga cggcaatgcc cgaatccgac agccgccgcg
    14641 ccagacggca ggagaacgca taatccgcat gattctgcgg cgtgcgcgcg ctgccgaaga
    14701 tactgactgc cggaaacacg cccgccaatg cttcgtctgc ctgcctgcgt tcggcatcat
    14761 aacgtgcctg ctccggcaca cggtttgtat tctccattcc atcctccgtt caaaaacagc
    14821 gattgtacac cgtcaaaaa
//
LOCUS       DAWFLT010000049        14822 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWFLT010000049 DAWFLT010000000
VERSION     DAWFLT010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14822
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.049"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(210..1622)
                     /gene="trkA"
                     /locus_tag="WGR74_001400"
     CDS             complement(210..1622)
                     /gene="trkA"
                     /locus_tag="WGR74_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ0905805.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(1720..3243)
                     /locus_tag="WGR74_001401"
     CDS             complement(1720..3243)
                     /locus_tag="WGR74_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ0905806.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPPQWQAKIGIIPVKS"
     gene            complement(3550..4356)
                     /locus_tag="WGR74_001402"
     CDS             complement(3550..4356)
                     /locus_tag="WGR74_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ0905807.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            complement(4408..4659)
                     /locus_tag="WGR74_001403"
     CDS             complement(4408..4659)
                     /locus_tag="WGR74_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905808.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            4759..5520
                     /locus_tag="WGR74_001404"
     CDS             4759..5520
                     /locus_tag="WGR74_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905809.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDASVP
                     KLDDASRNRVLKAYLKKWQEEHNAAVADCGEEIETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRIRFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDIRKILNDIKYELKYKR"
     gene            5818..6987
                     /gene="metZ"
                     /locus_tag="WGR74_001405"
     CDS             5818..6987
                     /gene="metZ"
                     /locus_tag="WGR74_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ0905810.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALESAERAVATSTGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGAILVVDNSLLSPIGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQAAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAALIRKQQNGGGIVIGFEVADQEAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            7073..7279
                     /locus_tag="WGR74_001406"
     CDS             7073..7279
                     /locus_tag="WGR74_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ0905811.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            complement(7378..7490)
                     /locus_tag="WGR74_001407"
                     /pseudo
     CDS             complement(7378..7490)
                     /locus_tag="WGR74_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(8412..9164)
                     /locus_tag="WGR74_001408"
     CDS             complement(8412..9164)
                     /locus_tag="WGR74_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0905812.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQNTAAHINLHFGNAAEQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQDPAYQYTGKSTRFDVYLPYGTDKG"
     gene            complement(9268..9735)
                     /locus_tag="WGR74_001409"
     CDS             complement(9268..9735)
                     /locus_tag="WGR74_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ0905813.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            complement(9787..11304)
                     /locus_tag="WGR74_001410"
     CDS             complement(9787..11304)
                     /locus_tag="WGR74_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEZ0905814.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYVWLAIACWLIFFSIIHPHWQWQSLKMPRFSAVADITMIGVLTYLF
                     GGIDSGFGILILPFVVCSCLLSYGRYPLLYSSYAAILLIFNAIADGDIGKYPLISDAR
                     TASATFILVAASYLSAIFTSLSVKYIDRAGKLAYDSHIAYHRIKGLSQTVLERVQEAV
                     VVINAEGLAVLFNRKAKDLFPALEIGRRAGLSDSAAELWDQASPHTFEYVLGTPGLNA
                     GIRAVPVNKGSDKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIRHA
                     NDLLRENMEAGAADPFNAKLCKIIDGNICRIDKMLEDISSLNKRNKTERETIGLIPFW
                     EEFKQEFLLGHPDAADCIRPDIQGGSPTAYFDPAHLRQIMWNLANNAWRHSRKQPGSI
                     SVTIRPAQKNTVCILFADRPKCRNTCSNPFTPRRKTAPASGCMSPANWRTPISAI"
     gene            complement(11325..13628)
                     /gene="parC"
                     /locus_tag="WGR74_001411"
     CDS             complement(11325..13628)
                     /gene="parC"
                     /locus_tag="WGR74_001411"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ0905815.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSLGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATSQNR
                     AIAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEAEILIESEGRRGAAHKDRIPI
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            13924..14607
                     /locus_tag="WGR74_001412"
     CDS             13924..14607
                     /locus_tag="WGR74_001412"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ0905816.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVIPDGENLQVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
BASE COUNT         3117 a         3682 c         4382 g         3641 t
ORIGIN      
        1 tgaatattgc cgctgtccaa tggtttggat tcccgcctgt gcgggaatga cgatttggaa
       61 attacccgaa acccaaaaac aactgaaacc gaacagaccg gattcccgcc tgcgcgggaa
      121 taacggtggg ggacgatgcc gtctgaagcc cacctaccat tattccgctt ccataccgct
      181 tggcggcaat gtgtccggaa tggggcggtt tatccgaaaa agcccatttt gacttggatg
      241 agtttttcca gttcgttcag gatgcgccgg cgcgagacga aaaagatgat gtggtcgccg
      301 tcttggatga cggtttcggt atggtgtccc ataatggttt cgccggttcc ggcgcggacg
      361 acggcggcaa tgtggcagcc ttcgggccat ttgatgccgc tgatgcgcct gccgatgatg
      421 gcggaagttt ttttgtcgcc gtgtgcgacg acttcgatgg cttccgccgt gccgcgccgg
      481 atggggtgga cggcaacgat gtcgccgcgc cggatgtggg cgagtatcga gccgatggtg
      541 atgaggtggg gggagacgac gatgtcgatt ttgttgcctt cgagcaaatc gacgtagctt
      601 gagcggttga cgatgccgat gacgcgcttc gcgccgaggt ttttcgccaa aagggcggac
      661 ataatgttgc tttcgtcgtc gttggtcagg gcgcagaata cgtcgatttc gtcgatgtat
      721 tcgttgtcga gcagggtttc gtcggttgcc gaaccttgca ggacgagggt gttgtcgagg
      781 ttttcggcta tccattcggc acggcgcggc cggcattcga tgattttgac gttgtatgcg
      841 tgttcgagct gcttggcgag acggtagccg atgttgccgc cgccggcaat catgatgcgg
      901 cgggtgctgg tttctttggg gcgcaattcg ggtatgaccg cgccgatgtt ttcggcggcg
      961 gcggcaaata ggatttcgtc gccttcgatg atgacggttt gcggcgcggg gacgatgagg
     1021 cggttgttgc ggtaaacggc gcagatttgg cagtcggccc cgtcgggcaa atcttgggcg
     1081 atgtcggcaa tgctgcgtcc gacaagcagt ccgccgcgcc gcgcctgtat gatgaccatc
     1141 cgcacgcggt cgtctgcaaa acgtaaaacc tgcaatgcgc ccgggcagtc tatcaggccg
     1201 gcaagctgtt cggtaacgag ctgttcgggg ctgatggttt cggttatgcc gaatatggaa
     1261 aggctgccgt tttcgttgtt ttcgagcttg gggctgaggt attcgaggta ttcgctggaa
     1321 cggacgcgcg cgatgcggcc ggggatgttg aacaggtcgg cggcaacttt gcaggcgacg
     1381 atgttggttt cgtcgctgcg ggagagcgcg agcagcaagt cggcatcttc cgcgccggcg
     1441 cgttctaatg tgaaggggga tgcgccgttg ccgaaaacgg tttggacatc gaggcggctg
     1501 cctgtttcct gcaatgcttt ttcgtcgatg tcgataacgg ttacgtcgtt gttgggtatg
     1561 gcggcaaggt tttgtgcgac ggtagaacct acctgtccgt tgcctaaaat gaggattttc
     1621 acggtgtggg tcgccgagtg atgaaatggt ggattgtacc gcaaccaggg gcggaaatgc
     1681 cgtctgaaag cagattttgt gttcagacgg catgacgcct caagatttga cggggatgat
     1741 gccgattttc gcctgccatt ggggcggggc ggtggcgtgg atggattcgc ctttgctgtc
     1801 tacggcgacg gttacgggca tgtctttgac ttcaaattcg taaatggctt ccatgcccaa
     1861 ttcggggaac gccaagactt tggaagattt gatggctttt gccacgagat acgccgcgcc
     1921 gccgactgcc atgaggtaca cggctttgtt gtcggcgatg gcttcgcagg tggccacgcc
     1981 gcgctcggat ttgccgatca tgcccaagag gtcggtttgt tcgagcattt ggcgggtgaa
     2041 tttgtccatg cgggtggctg tggtcggacc tgccggaccg acgacttcat cgccgaccgg
     2101 atcgacgggg ccgacgtagt aaatcaggcg gttggtgaaa tcgacgggca attcttcgcc
     2161 tttgttgagc atatcgacga ggcgtttgtg tgcggcatcg cggccggtga ggattttgcc
     2221 gttcaacagc aatacgtcgc cggttttcca gcttgccact tcttctttgg tcagcttgtc
     2281 gacatcgacg cgtttgccgt tgtcggggct gtaagtcaaa tcgggccagt cttcgacgcg
     2341 cggcggcgtg agttcgacag ggcctgagcc gtccaattca aattcgacgt ggcgggtggc
     2401 ggcgcagttc ggaatcatgg caatcggttt ggaggcggcg tgggtcgggt aatcgaggat
     2461 tttcacgtcc aacacggtgg tcagaccgcc caagccttgc gcgccgatgc ctagcgcgtt
     2521 gactttttca aagagttcga ggcgcagggc ttcggtggtg gacaattccg cgccggacgc
     2581 ggctttttcc tgcaattctt ggatgtcgat gtggctcatc agggattctt tcgccatcaa
     2641 cacggctttt tcgggcgtac cgccgatgcc gatgcccaag atgccgggag gacaccagcc
     2701 cgcgcccatg gtcgggatgg ttttcaatac ccaatcgacg atgttgtcgg aaggattgag
     2761 catggcgagt ttggatttgt tttcagagcc gccgcctttt gccgcgcagg ttacttcgac
     2821 tttgtcgccc ggcacgatgc tcatatggat gacggcgggc gtgttgtctt tggtattttg
     2881 gcgtttgccg gcggggtcgg cgagaacgga agcgcgcagc gtattgcctt cccaagtgta
     2941 ggcgcggcgc acgccttcgt taaccatttc ttccacgctc atatccgcat cccactgcac
     3001 atccataccg actttcaaaa acacggttgc gataccggta tcttggcaga tggggcggtt
     3061 gttttccgca cacatacggc tgttgaccaa aatctgcgtc atcgcgtctt tggcggcggg
     3121 attttcttcc ttctgccacg ccttataaag cgcgtcgatg tagtctttcg gatggtagta
     3181 gctgatgaat tggaaggcat cgcagatact ttggataaag tcttcttgct tgatgacggt
     3241 catgattgtg ttccttttca aggttgtggc ggtaagtgtc tgctgctgtt ttaagcaacc
     3301 tttttgaagg ctgtttttta tcaggttgaa atagtgccag cgtttgtacg gtaaagacaa
     3361 tacataattc taatctcttg attatcaatt tgttttaata aaatactgtt ttactcggat
     3421 tttgatattt gtaatctttg cctgttgctt tgcggtaggg agaaatcagc aggcagttca
     3481 ttgtaggccg attgatgaac aatgccgtct gaaagggctt cagacggcat tgtgttcgga
     3541 cgggcgggct tattttgcgg cctgtccgtc ttcttttgca aaatatttgg cgtaaatctt
     3601 gtcgtattcg ccgctttcgc gtactttttc caacgcatcg ttcagcattt tgacggttgc
     3661 ttcgtcgcct ttgcgtaccg cgatgccgta gtgttcggtg gtgaagtcgg gcagggtaac
     3721 gaagtccatc cctttggtcg gattgttttt cacataattg gcgatgaccg cgctgtcgct
     3781 gactacggaa tccaagccgc cgttttccag ttctttgata atcaggggaa cgttttcaaa
     3841 gcgcgcgatt ttcgggttgt cgttgcccaa gagtttggat acggagaaat cgcccgtgta
     3901 gccggttacc acgccgactt tgttcatgtt tttcaaatcg tcggaagaag atattttttt
     3961 gcctttcgga acgaggacga cttgggtgat ttcaaaatac gggtcgctga agtccataga
     4021 ctgtttgcgg tcgtcggtaa tggttacgcc cgacatcaca acgtccgcat cgccgttgtt
     4081 caaggcgggg aaaaggctgt cccacggctg gtgtttgaat tcgattttaa aattgcccgc
     4141 cttcgccatc gcgttcatca aatccacatc gaaaccttcg acattgcctt tcgagtctaa
     4201 agattcaaag ggggcaaact cggcgttgga agccacgcgg tacactttgt cggggtttgc
     4261 ggcgggcgcg gcggcatctt tgccctgacc gccgcaggca gacagcgcga gtgcggaaca
     4321 ggcaagggcg gcggcaatcc attttttcat attcatcaaa attcctttcg gtaatcaaaa
     4381 agcctaaaaa atcagtttaa cacaaaatta acgttctttt tcacggaaaa acgcataagt
     4441 aacgtcgtgg aacaaatcgg tatgggggct gtcctcatag ctcaattctg ccaaggcgtc
     4501 ctcaaaagca agctgaatcg actcgaccgc gatttcggcg gtttcggcgg gaagggcgcg
     4561 gattaaggct ttgaatgcgg tggacaggac gtggttctgc atacgcagca cgtcgttcgc
     4621 ctcttccaga tattccaaac gctgttcgat gctgcccatg atgtatcctc gcgaatctgt
     4681 taaaatgcgc cattatatca aaccgcgctt tgggcgcggg tttgtctgcc tgcaaagatt
     4741 aagaaccgga agccgaaaat ggatgcgaat aaagagccga attgggaaaa acgtttggaa
     4801 aacctgcgtc agacgcgccg tatgaaggca gtgccgactt acgcgccgct gtcccgccct
     4861 tctgaaaccg gaacggacgc gtctgtgccg aaactggacg atgcaagccg aaaccgtgtg
     4921 ttaaaggcgt atctgaagaa atggcaggag gagcacaatg ccgccgttgc cgattgcggg
     4981 gaagaaatcg aaaccgaccc gatgatcgtc cttcaggaaa actggctcaa cgcgcaggct
     5041 tccctgcaaa tgtcggtttc ggaaaaacac atcgaatcgc ggcggagaat ccggtttgat
     5101 gccaaaaaag acagtgccga atttgaaacc ggacagattg aaggcgggga aaacgccgat
     5161 gcggcagaat cgggtacggc ggcagacggg gttgcggaaa acggcgaagg cggtgcggaa
     5221 gaagcgttga tgcccgtcca aatcaatatc ctgaacccga aagccgtcaa ccggcgcgaa
     5281 gtgttctgcc tgtccgaaca ggagctgacc gagcggctga tcaaacgcct gcgcccgcat
     5341 ctgaccgata ccgtcaacgg catcatccgt acggcggtgc aaaagcagat ggcggttttt
     5401 acctaccagc tccagcagat gctgcacgag caggcgggtg cggtagtcga agacgtgttg
     5461 gaacacgata tccgcaaaat cctcaacgac atcaagtacg agttaaaata caaacgctga
     5521 ttgccatgcc gtctgaaagc cgtttcagac ggcattttcc tgcccgtccg acttgaaagc
     5581 ctcatggaac aggcgcaaaa tattaatata gtggattaac aaaaatcagg acaaggcgac
     5641 gaagccgaag acagtacaaa tagtacggaa ccgattcact tggtgcttca gcaccttaga
     5701 gaatcgttct ctttgagcta aggcgaggca acgctgtact ggtttttgtt aatccactat
     5761 aaattaaaca acgggcgcac gctccgttgc cgaacggata aaaccgagga atcaccgatg
     5821 agcaaaaaac tccatccgca aacgctcgcc atacgcggcg gcaaagaaca aaccggatac
     5881 cgcgaacaca atcaggcatt gttcctgacc agcagtttca tgtgggacaa cgcccaacac
     5941 gctgccgatt tgttttcaaa aaaaatcaaa ggcttcactt atacccgtac cgccaacccg
     6001 accaccgccg cctttgaaaa acgcatcgcc gctttggaaa gtgcggaacg cgcggtcgcc
     6061 acttcgaccg gcatgtccgc gattcaggcg gcgtttttta cctttttgca ggcgggcgac
     6121 catgtgattt ccagccgcag cctgttcggc acgaccgtcg gctttatcaa taacatcgtt
     6181 accaaattcg gcatcgaagt gagccacgtg tcgccgactg atataaacga atggaaagcc
     6241 gccgtcaaag ccaacaccaa actgctgttt ttggaaacgc cgtccaatcc cttgggagaa
     6301 gtggccgact tggaagcctt ggcggaattg gcgcacggca tcggcgcgat tttggtggtg
     6361 gacaacagcc tgctgtcgcc catcggctct cagcctttga agcacggcgc agatatttcc
     6421 gtttcttccg caaccaaagc catagacgga cacggacgcg tgatgggcgg cgtgttggcg
     6481 ggttcggaag aactgatggc gcaggccgcc gtttactgca actcttgcgg gctggcgatg
     6541 tcgccgttta acgcgtggca gttgttgagc ggcgtggaaa ccctgtcgct gcgtatggaa
     6601 aaacagttcg acaacgccct gaaaatcgcg caatggctgc aagcgcagcc gcaggttcaa
     6661 gccgtgtatt acaccggatt gcccgaccat ccgcaggcgg cgcttatccg caaacagcaa
     6721 aacggcggtg gcatcgtcat cggctttgaa gtagccgacc aagaagccgc gtggaaagtt
     6781 gtggacggcg tggaactctt ttcccgaacc gccaacctcg gcgacgtgcg ctcgaccatc
     6841 acccatccgt ggacaaccac gcacggcagg atgcagcccg aagaaaaact cgccgccaac
     6901 atccgcccgg gactggtgcg cctgtcggtc ggtttggaat atgttggcga tttgattgac
     6961 gatttgaaac aggcactcgc acgctgattg cgtgtttcag acggcattgg acagcagatg
     7021 ccgtctgaaa cccttatcgc gtatggcata cacatcaaca ataggaaaaa atatggatga
     7081 agcagttttt gccgattggg cgttgaaaat ctgcctgacc ggcttgatta tttttttggg
     7141 ttttatcgtt tggaatttgg gcaaggagtc caaagcgggc aaattcggca tcgccatttt
     7201 gtttttggtt ttgggcttgg gcgtgttcgg ttttgtgttc aaagagttat tgattaagtt
     7261 tttggtattg ccgaaatagg tttgcggcct gtcaaaatgc cgtctgaaat cataaaaagt
     7321 aatctgcacc ctaaaagttt gatttaaccg ccaactgatt aaggtgcgga tttttatgac
     7381 aaaatatagt ggattaacaa aaaccagtac ggcgttgcct cgccttgccg tactatttgt
     7441 actgtctgcg gcttcgtcgc cttgtcttga tttaaattta atccactata aaaatcatct
     7501 ttccgcagtc attcccacgg acgcgaaagt ctgtcttttt gaatcccgga taattttaca
     7561 tcgtcattcc cgcgcaggcg ggaatccaaa tcattgggta gcggcaatgt tcaaaagtcg
     7621 tctgaaaaat cagaagttct agattcccgt tttcacggga atgacggaat ttcagacggc
     7681 atcctcccgc cccgtcattc ccgcgcaggc gggaatctag tccgttcggt ttcggtcatt
     7741 tacgataaat tcctgttgct tttcatttct ggattcccac ttccgtggga atgacggagg
     7801 agcggataaa ttctcgcaat ctaaaacctt gtcatttcca taaaaaacag caacccgaaa
     7861 cagcaactta aaaacagcaa cctgaaacct cgtcattccc gcgcaggtgg gaatccagat
     7921 cattgggtgg cggcaatgtt caaaagtcgt ctgaaaaatc agaagttcta gattcccgtt
     7981 ttcacgggaa tgacggaatt tcagacggca tctgcccgcc ccgtcattcc cgcgtaggcg
     8041 ggaatctagt ccgttcggtt tcggtcattt acgataaatt cctgttgcct ttcatttcta
     8101 gattcccact tccgtgggaa tgacggcgga gcggataaat tctcgcaatc taaaacctcg
     8161 tcatttccat aaaaaacagc aacccgaaac agcaacctga aaccttgtca ttctcgcgca
     8221 ggcgggaatc tagtccgttc ggtttcggtc atttacgata aattcctgtt gcttttcatt
     8281 tctggattcc cacttccgtg ggaatgacaa actttaaggt gtgatgattt atccaaaaca
     8341 gtcgaaacgc aaaaaccggt ttctcgtttg cataagaacg gcaaattttt cggtgtcttg
     8401 ttttatgggc gttatccctt gtccgtcccg tagggcaggt aaacgtcgaa gcgggtgctt
     8461 ttgcctgtgt attggtaggc agggtcttgt ccggcaaggt gttcgccgag gcgggggcgt
     8521 ttgacgacga cgcgtttttt tgctgtttgg cgtgcggtat gcaggagtgc cgcttcatct
     8581 tgcgcttcgc cgacgagccg gtggaagtag gtcatttctt ttttaacggc ggcacttttg
     8641 cggcgttcgg gatacatggg gtcgagatag acgatgtcgg gtttgccttg tgtttggaca
     8701 agtgcgggca tttgttcggc ggcgttgccg aaatggaggt tgatgtgcgc ggcggtgttt
     8761 tgcgtttcgg gattgaggag ggcgcggcgg atgccgtctg aaagcaggca ggcgacggcg
     8821 ggatgttgct cgaaggcggt aacggccagc ccgagcgagg cgaggacgaa gctgtcgcgc
     8881 cccaatcctg cggttgcgtc ccaaacggtg gggtgcgcgg tgtggttgac ggctttggcg
     8941 atgagttcgc ccccgccttt tgtgcgccgg tattgtgccg cgccggaggc aaaatcgacg
     9001 atgacgctgc ttttttcccc gacaagcctg aggctgacgg tatcgtgttc ggcaaggagg
     9061 taactgcctt gttcgggcgg ttgggaaacg ggcacaaggg ggaatgcccg tatcagggtg
     9121 cggacggttt cggtggcggt gttgtcaata aggatgtcgg tcatggtgtt ttgtgtccgt
     9181 tcgggcgtgg ggcgaacccg ctttcccgtg ccgtttcaga cggcattttt tcgggtgggc
     9241 ggagatggtg cggatgccgt tttgtcgtca gccgatgttg aggcgttcca tacggtagcg
     9301 catggaacgg aagctgatgc ccaagcgttt ggcggcctgc gtgcggttgc cttcggtttg
     9361 tttgagggtt tgttcgatga tgtcgcgttc gatttggtcg agatagtcct gtatctgcat
     9421 ggtatcgggg tcgaacgcgc cgcaacgcaa cgcttcctgt gcctgatcgg cgttgccgaa
     9481 ggcggtgatg acggcaaccg gcgtatcgag caggcggctg ttgatgtgtt ggacgacttc
     9541 cagccccgag ccgtccggca tacgcatatc ggtcaggacg agcgaatagc gttggctgtc
     9601 gagcttgttt ttggcttcgg caacgcctga cgcggtatgg acgcgcaagc ccattttcat
     9661 cagggtcatt tccatcaggt cgagaatgtc ggtttcgtca tcgacgacga ggacggggtg
     9721 ttgcagttca gtcattggtt ttttccggta atgtgagttc gaaacatttg gcttccggta
     9781 ggtaggtcaa atcgccgaaa ttggcgtgcg ccagttcgcg ggcgacatac agcccgaggc
     9841 cggtgccgtt ttccgccgtg gtgtaaaagg gttcgaacag gtgttcctgc acttcgggcg
     9901 gtcggcaaag aggatacaga cggtgttttt ttgcgcgggg cggatggtga cggaaatcga
     9961 gccgggctgt ttgcggctgt gccgccacgc gttgttggcg aggttccaca taatttgccg
    10021 caggtgggcg ggatcgaaat aggcggtcgg gctgccgcct tgaatgtccg gacggatgca
    10081 gtcggcggca tcgggatggc cgagcaggaa ctcttgtttg aattcttccc aaaacggtat
    10141 caggccgatg gtttcgcgtt cggttttgtt gcgcttgttg agcgaggaaa tgtcttcgag
    10201 cattttgtcg atgcggcaga tgttgccgtc gatgattttg cacaatttgg cgttgaacgg
    10261 atctgccgcc cccgcttcca tattttcgcg cagcaggtcg ttggcgtggc ggatggcgga
    10321 catcgggttg cggatttcgt gggcgaggtt ggcggtcagt tgtccgagcg cggcaagttt
    10381 gacggacagg gcttctgcct gaatttcgct ttgcgggcgg atgtagagga tgagcagctt
    10441 gtccgaccct ttgttgaccg gaacggcgcg gatgccggcg ttcaggccgg gtgtgccgag
    10501 gacgtattcg aaagtgtgcg gagaggcttg atcccacagt tcggcggcag aatcggacag
    10561 accggcgcgc cgtccgattt cgagcgcggg gaaaaggtct ttcgccttcc ggttgaacag
    10621 caccgccagc ccttcggcat tgatgacgac gacagcttcc tgaacgcgtt cgagtacggt
    10681 ttggctcaag cctttgatgc ggtggtaggc gatatggctg tcgtaggcga gttttccggc
    10741 acggtcgatg tatttgaccg acagtgaggt gaagatggcg gaaagatagg aggcggcgac
    10801 aaggatgaag gttgccgagg cggttcgggc atccgatatg agcgggtatt tgccgatatc
    10861 gccgtcggca atggcgttga atatcagcag gatggcggcg tagctggaat agagcagggg
    10921 gtagcgcccg tagctgagca ggcaggagca gacgacgaag ggcaggatca ggatgccgaa
    10981 gccggaatcg atgccgccga acaggtaggt cagcacgccg atcatcgtga tgtccgccac
    11041 tgcgctgaaa cgcggcattt tcagcgactg ccattgccaa tgcggatgga taatggaaaa
    11101 gaaaatcagc cagcaggcaa tggcgagcca aacataaaac gcccacgccg tccacgaagc
    11161 gtgcaggttc gcacggctgc cggtttccag cccgaggatg tgcataatca tcagcgggaa
    11221 aacgatggcg acgcggatga tgttgatcag attgggaatc cggtctttga gtttttcaag
    11281 ttcgcggggg ttggaaatta ccataattat gtgcggaatc gggtttattt cggagaagaa
    11341 agctgtttca ggctgcccga tatgggcaat agtttgcctt ttttgccgcg ttttgcctca
    11401 agcagggaga tggggatgcg gtctttgtgc gctgcgccgc gcctgccttc gctttcaatc
    11461 aggatttctg cctcggaaga aacggcggta tgcgtcatcg attcgccggc gtttaatccg
    11521 atgatttgca gtcctttgcc ttttgccata attttcaatt cgccgatggg gaaggcgatg
    11581 gcgcggtttt gactggtggc ggcaatgatt ttgcaatcgg ggttgatgaa cgaggaggca
    11641 tagacggcaa cgggcggcag gacggtttcg ccactgtctg cggtcatgac cactttgccc
    11701 gctttcacgc gcccgaccat atcgcccagt ttggcaatga agccgtagcc gccgctgctt
    11761 gataataaat aatgttgttc cggcaatcct gtcaacatcg caacgggttt cgcgccgttt
    11821 tgcaactcga tcaaggatga aaccggcacg ccgtcgccgc gtccgccggg aatttcggcg
    11881 gcatcgagtg tataggttct gcccaatgaa tcgaggatga cgacgggtaa aaccgttctg
    11941 ccttcaaggg tttgtttgag gcggtcgcct tctttgaacg cggtttggct caaatcgaga
    12001 ttgtgtccgg cacggctgcg tatccagcct ttttccgaca gaatcagtgt gatgggttcg
    12061 tcggcggtgg tttgtgtcag cacggcgcgt ccggcctctt ccaccagcgt gcggcgtgca
    12121 tcgccgtatt gtttcatatc cgcctgcatc tctttgataa tcagcttgcg cttttcgttt
    12181 tcatcgccca aaaggatgtt cagacggcct tgttcttcgc gcaattcgtt caattctttt
    12241 tcgagtttga aaccttccaa acgtgccaac tggcgcaggc ggatttccaa aatgtcttcg
    12301 gcttggattt cggtcagccc gaacgccgcc atcaaatcgg ctttcgggtc atctgattcg
    12361 cggatgactt taatcacttc gtcgatgtgc agaaagactt tcagacggcc ttcgaggatg
    12421 tgcagccgtt tttccacttg gttcaaacgg aatttcagac ggcgtgttac ggttacggtg
    12481 cggaaatcca gccattcctg caaaatcgtt ttcaggtttt tctgcgcggg gcggttgtcc
    12541 agccccatca tcaccaagtt catggacaca ttgccttcca gcgaagtttg cgccatcagc
    12601 gtgttgatga aggtatcggt atcgatgcgg ctggatttcg gttcgaacac cagtcgcacg
    12661 ggatgttcgc cgtcggactc gtcgcgcacg cggtcgatta aatccagcat cagctttttg
    12721 gtgttgagct ggtcttggtt gagctgtttc ttacccgctt tcggtttcgg gttggtttgc
    12781 tcttcgattt cggcaaggat tttggcggaa ttggcgttcg gcggcagctc ggttacgatg
    12841 acgcgccact gtccgcgcgc caatttttcg atttcataac gcgcacgcac gcgcacgctg
    12901 cctttgccgg tttcgtaaat acggcgcaat tcgtccgccg gcgtgatgat ttgaccgccg
    12961 ccggcaaaat caggtgcggg aatgtattgc atcaggtcgg cggtttccag cgtcggcttt
    13021 ttcaacaacg caatcgccgc ctgcgtcact tcgttcaaat tgtgcgacgg aatctcggtc
    13081 gccatgccca ccgcaatgcc tgacgcgccg ttgagcaaca ccataggcag gcgggcgggc
    13141 aggtgcagcg gttcgtcaaa cgcgccgtcg tagttcggca caaaatccac cgtcccctga
    13201 ttgatttcgg acaacagcaa ttccgcaatc ggcgtcagcc gcgcttcggt gtaacgcatc
    13261 gccgccgccc cgtcgccgtc gcgcgagccg aagttgccga tgccgtcgat taagggatag
    13321 cgcaaggtaa aatcctgcgc catccgcacc atcgcctcat aggcggaact gtcgccgtgc
    13381 gggtggtatt tacccaaaat ctcgccgacc acgcgcgccg atttcaccgg cttcgccccc
    13441 gccgtcaaac ccatatcgcg catggcaaac aaaatgcgcc gctgaacggg cttttggccg
    13501 tctgaaactt caggcagcgc gcggcctttg accacgctca tggcgtattc gagataggcg
    13561 cgttcggcgt atcggccgag catcagcgta ttggaatcgg tatgtggagc gtgcggttgc
    13621 atatttatcg tatttccttt tcggttgaac ccccgccact tggacatctg tccttcgggg
    13681 cggtagaatc agactttatt tgggaggggg caaccccttc caaatcaggg caacacatag
    13741 ggcggtgctt tatgtgtcgt cctgtgtgtt gaaacattgt gtatgcggaa tgtaaagcaa
    13801 agggcattat tgtaaatcaa aacggcaaac catagtgggc gcgtgtctga atctgaatca
    13861 aatacgggaa tgtgaaaata tgttataaat aaggctttcc cactttcaca cattggagac
    13921 gatatggaat tggtatttat ccgccacgga caaagcgaat ggaacgcgaa aaacctgttt
    13981 acaggctggc gcgacgtgaa gctgagcgag caggggcttg ccgaggctgc tgccgccggt
    14041 aaaaaactga aagaaaacgg ctatgagttc gacatcgcct tcacatccgt cctgacccgc
    14101 gcgattaaga cctgcaacat cgttttggaa gaatccgacc aactgttcgt accgcaaatc
    14161 aaaacatggc ggctgaacga acgccactac ggccgactgc aaggcctgga caaaaaacaa
    14221 accgccgaac aatacggcga cgagcaagtc cgcatctggc ggcgcagcta cgacaccctg
    14281 ccgccgcttt tggacaaaga cgatgagttt tccgcacaca aagaccgccg ctatgcccat
    14341 ctgcctgccg atgttatccc cgacggcgaa aacctgcaag taacgctgga gcgcgtattg
    14401 ccgttttggg aagaccaaat cgcccccgcg attttgagcg gcaaacgcgt cttggtggcg
    14461 gcgcacggca actccctgcg cgcgctggca aaacacatcg agggcatttc cgacgaagac
    14521 atcatgggct tggaaatccc gaccggtcag ccgttggtgt acaaattgga cgacaatttg
    14581 aaagtcatcg aaaaattcta cctgtaaggg tgtgaaataa aaaatgccgt ctgaaggctt
    14641 gagcgttcag acggcatttt tgacggtaaa cgtagcaact gctttcgcga gaacgacggg
    14701 attttaggtt tttgtttttc tgttttcgtg ggaaggatgg gttttaggtt gcggtcattt
    14761 atcggaaaag cagaaaccgc tccgccgtca ttcccgcgca ggcgggaatc tagaacctct
    14821 ga
//
LOCUS       DAWFLT010000050        14755 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWFLT010000050 DAWFLT010000000
VERSION     DAWFLT010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14755
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.050"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(74..1495)
                     /locus_tag="WGR74_001413"
     CDS             complement(74..1495)
                     /locus_tag="WGR74_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ0905817.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(1853..2212)
                     /locus_tag="WGR74_001414"
     CDS             complement(1853..2212)
                     /locus_tag="WGR74_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ0905818.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2209..2814)
                     /locus_tag="WGR74_001415"
     CDS             complement(2209..2814)
                     /locus_tag="WGR74_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ0905819.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDTSAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2861..3343)
                     /locus_tag="WGR74_001416"
     CDS             complement(2861..3343)
                     /locus_tag="WGR74_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ0905820.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3547..3644
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3855..4955
                     /gene="gcvT"
                     /locus_tag="WGR74_001417"
     CDS             3855..4955
                     /gene="gcvT"
                     /locus_tag="WGR74_001417"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ0905821.1"
                     /translation="MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLGKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEADVRVLKLPFVRNGQKQFD"
     gene            5118..5504
                     /gene="gcvH"
                     /locus_tag="WGR74_001418"
     CDS             5118..5504
                     /gene="gcvH"
                     /locus_tag="WGR74_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ0905822.1"
                     /translation="MSSNIPAELKYVASHEWLRLEEDGIITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDPYGAGW
                     FFKLKPANVADYDVLLTAEQYAGEVD"
     gene            5656..6903
                     /locus_tag="WGR74_001419"
     CDS             5656..6903
                     /locus_tag="WGR74_001419"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ0905823.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAACLPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEISPYLYTLGMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHEYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIRALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSIQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            7179..7604
                     /locus_tag="WGR74_001420"
                     /pseudo
     CDS             7179..7604
                     /locus_tag="WGR74_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            7610..8840
                     /locus_tag="WGR74_001421"
                     /pseudo
     CDS             7610..8840
                     /locus_tag="WGR74_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777561.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            8898..9518
                     /locus_tag="WGR74_001422"
     CDS             8898..9518
                     /locus_tag="WGR74_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0905824.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVNQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            9529..9660
                     /locus_tag="WGR74_001423"
                     /pseudo
     CDS             9529..9660
                     /locus_tag="WGR74_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            9657..10151
                     /locus_tag="WGR74_001424"
     CDS             9657..10151
                     /locus_tag="WGR74_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ0905825.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTKWIDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKDGKVVSFDDMPDT
                     YIFK"
     gene            complement(10285..11946)
                     /locus_tag="WGR74_001425"
     CDS             complement(10285..11946)
                     /locus_tag="WGR74_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ0905826.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEVGAHSLAGAVIDPIALNELISDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GIGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGILVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(12076..12619)
                     /locus_tag="WGR74_001426"
                     /pseudo
     CDS             complement(12076..12619)
                     /locus_tag="WGR74_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(13553..14206)
                     /locus_tag="WGR74_001427"
     CDS             complement(13553..14206)
                     /locus_tag="WGR74_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0905827.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLVLAADEVFEGPVEQDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTDSLSSYDKLDVSGFIHYRINHSKECADRQ
                     NHINGIENFWNQAKRVLRKYNGIDRKSFPLFLKECEFRFNFGTPSQQLKILWDLGLI"
BASE COUNT         3731 a         3679 c         3871 g         3474 t
ORIGIN      
        1 gtcggaaggg gggatattga tttgattatg ccggaattta aaatgccgtc tgaatgttca
       61 gacggcatag cgtttacagc agtttgaaaa cgaaaaagat aagggtatgt acgatgaaga
      121 cgggtgtcag gaaggcgacc gaccacatca tatagccgaa gaaagtcggc atcggtacgc
      181 cgcgctgttc ggcaatggcc ttgaccatga agttcggtgc gttgccgatg taggtcagtg
      241 cgcccatgaa taccgaaccc atagaaaccg ccagcagcga atgaaacagg gtacccgtca
      301 tcaaggcttg ggcatcgccg cccgccatat tgaaaaaaac gagataagtg ggcgcgttat
      361 ccaagaatgc cgacaatatg ccgctcatcc aaaaatacat cacattaatc ggatgacctg
      421 ccgtatcgtg aaccagcgat accaccccgc ccagcgcgcc tgcctcgcct gctttcagaa
      481 tgctcaggac gggaaagatg gtgatgaaga tgccgaggaa gagtttgccc acttcggcga
      541 tgggttcaaa gttgaattcg ttgcctgcgc ggacttgttt gggcgtgatt gccatagata
      601 cggcggtcaa tgcaatcagg atgacatcgc ggacgaggtt ttgcagggcg taacggctgc
      661 cgaggatttc aaatcccggg tgttcgggtt tccaaaggcc ggacattaga accgcgccga
      721 ccacgcccga aagcaggagg aagttccatt tgccgaagat ggcgattttt tcgggttttt
      781 cctgttgtgc cggcgtatct tgtgcaatgc tttcctgttt gaagaaacgg ttgtcgatga
      841 aatagaaggc ggtcaacagg acagcggtgc tgatcaggac gggggcgaac atatgtttga
      901 ccgtccacat gaaatctacg cctttgagga agccgaggaa gagtgggggg tcgcccaaag
      961 gggtcagacc gccgccgatg tttgcaacca ggaaaatgaa gaagatgacg atgtgcacgc
     1021 ggcgggtacg gttttggttg gctttcagca gcggacgaat catcagcatt gctgcgccgg
     1081 tcgttcccat gatagaggca agtgccgtac cgacggcaag cagggcggtg ttgagcttgg
     1141 gtgtgccgtt caagtcgccc caaaccaaaa tgccgcctga aatggtgtac agggcaagca
     1201 gcagcaggat gaaggggatg tattcttcaa cgagtgcgtg tgcgacggta tggataccgg
     1261 cggacgcgcc aaaaaccaaa ctgaacggga tgaggaagag caatgtccaa aaggcggtga
     1321 ttttgccgta atggtgatgc caggtatgcg aaaaaaacaa gggacccaat gcgatagaca
     1381 gcaaaatcag ggcaaagggc aggctccaca gcaggtttag gtttgcgccg tccaaatctg
     1441 cggcgtaaac cgatgcgggg aaaagcatta gtgaaaacag gggtaggtgg cgcatcgtgt
     1501 ttcctcgatt caagcactgc cttgcgcggc gcgtgggagt gatacaggca ccgtgccgcc
     1561 cggacatagg cggactgtgc tgcctgtttg tttttgaaaa aagttttccg attgattgta
     1621 aagtaaataa tcaggataaa ccagtttccc aaaccggaag gcggcgggaa ggcggattgc
     1681 tgtgcttggg aatatatgtt ctttttgata aataatttta tttaaaacaa ataaatatat
     1741 gtctttaata aataatctat cgaaaacgaa aaatgaattt attttaacat atatttgcaa
     1801 tgaaacaggt ttgccccccg tttgtttgcc cttatccctt tcagtacggc attcaagatt
     1861 cgggcctgcg ccacatccat atggcgacaa gggaacaaaa aaccgatgaa accgccccga
     1921 cccaccagcg ttggggaaac tgccaaaaca ttatcaggca ggatgcggtc atcatactga
     1981 tggcgaatat tttggctttg cgcggcactg cgccgttttg ttcccagtta tgaaccatcg
     2041 gaccgaaata gcggtgccgg tgcagccagc ggtgaaagcg cggggatgcc tttgcccagc
     2101 aggcggcgga gagcagtacg aacggcgtgg tcggcaacag cggcaaaaaa atgccgatga
     2161 tacccaacag cagggaaatg cagccgcagg caattaaaag ataacgtatc attttgaaat
     2221 atttttctta ttgtgcggat aagggcagga tgtgataccg agttttgccc agccttcatg
     2281 tcccattttt tccagcaggg cgatattgcg ttcgaatatt gccgaagtgt cgggaaaggc
     2341 ttgtgcggct ttggcaatgc tgtcttcgcg gatgaggtgc agcgtcggat agggagaacg
     2401 gttggtgtag ttgccgatgt cgtcgggttc ggtattctga aagcggaatt ggggatggaa
     2461 cggtgcgatt tggacgatgc cttccaagcc gttttcgata acggcggcat cggcaatatc
     2521 gagcatatcg ttgaatacgt cgaaatcggg gaatagggtc gggtgaacca gcagggtggt
     2581 ttccagttcg gtggcgggtg tattgcccag tcgctgcagt tcttcgtcca agtcttccaa
     2641 aaaactgtca aggtgtttgg cttcgctgat tgcgatgcgg acaaggtttt taacgtgggg
     2701 ggctttggca aagggacaca ggttcagacc gatgacggct ttttccaacc attgtccggt
     2761 gtgttcggca acagcatctt tattttcgga agtattgata ttcattattg tcatgtaaat
     2821 gtgtttgcag attgcacgtg cgggaaaatc gggaagggca ctattccttc agcaggtggt
     2881 tgagcggcag ggaggtggtg tgtttgattt cttttaaaac aaagctcgat tgcgcatctt
     2941 gtacgccgtg gtgggacagg agcgtatcca aaacaaaatg ggaaaacgcg ttcatatcgg
     3001 taaaaaacgc ctgaagcagg tagtcggttt cccctgtcag ggcgaagcag ctcaagactt
     3061 caggccattt tcggaccgat gcggcaaagt cttcccgcgc gtcttttgct ttgcggatgg
     3121 aaacgcggat aaatgcctga agccccaggt tgacggattc cggagacagc agcgcggcat
     3181 attggcggac gatgccggca tcttccaact gctttagacg gcgcaggcac ggagaaggcg
     3241 aaagtgcgac acgttcggac agttcgacat tggtcagcct gccgttttcc tggagaacct
     3301 gtaagatttt aatatcggtt ttgtctaaag tgagttgggg catatttgcg ttccgtttta
     3361 aggaattcgg attgtctgtc cgtatgtttg cggcaatccg cacagatgga gaccatatta
     3421 acatataaaa agttataccg tcatccggga caaattttgt tttcggaaaa tcatgtgaaa
     3481 acagaggcgg tcggtttgca tctcttttaa gacggcttgc ccaaaccgcc gattcaagac
     3541 ataatcggga aatgtgcagg agagtgttac acccaactac aatgtaacca ccgaaggcgc
     3601 agacaccctt aaatcgctca ggtatcaggg actgcacatt gaaacaaaca atctggagag
     3661 cggcgttgga ataacgtcca ccgaagggga gaaggccgtc tgaaccacca ttcagacaac
     3721 cgcgcaaagc agtgagcaga ctggtttgcc atcatgcgga tacggccgaa aatctcaggt
     3781 tcaaggacag atagggtcat ccgcgcacag gtgcgcgggt ggcatctgaa caaaaaatcc
     3841 ggagaaactt gagaatgatt actctgaaaa ccaccccatt tcatcaagcc catcaagatg
     3901 caggcgcgaa gccggtagat tttgccggct gggagctgcc catccattat ggttcgcaaa
     3961 tcgccgaaca cgaagccgtg cgcaccgacg ccggtatgtt tgacgtatcc catatgcttg
     4021 ttaccgacgt agcaggcgca aatgccaaag cctttttccg caaattgatt gccaacgatg
     4081 tcgccaagct cgcttttgtc ggcaaagccc tttattccgc tttgctcaac gacaacggcg
     4141 gcgtgattga cgacttgatc gtttaccgca ccaatgaagc cgaaacccaa taccgcatcg
     4201 tgtccaacgg cgcgactcgc gaaaaagata cggcacaatt ccacaaagtc ggacaagagt
     4261 tcggtgtttc cttcaatccg cgctacgacc tcggcatgct cgccgtacaa ggccctaaag
     4321 ccattgaaaa actcctgacc gtcaaacccg aatgggcaga tgtcgtccat aacctcaaac
     4381 cgttccaagg cgcggatttg ggcaacgact ggtttgtcgc ccgcaccggc tacaccggcg
     4441 aagacggcgt cgaagtcatc ctgcccggca ccgaagccgt cgcattcttc aaagccctgc
     4501 aagcagcagg cgtacaacct tgcggtctcg gcgcgcgcga caccctgcgc atggaagccg
     4561 gcatgaacct ctacggcaac gatatggacg acgataccag cccgctcgaa gcaggcatgg
     4621 gttggaccgt tgatttgaaa gacgaaagcc gcgattttgt cggcaaagct gccttgttgg
     4681 cattgaaaga aaaaggcgtt gccgtcaaac aggtcggttt gctgctcggc aaaggcggca
     4741 tcctgcgcgc gcatatggaa gtgttgaccg acaaaggcaa aggcgaaacc accagcggcg
     4801 tattctctcc aagtctgaaa caatccatcg ccatcgcccg cgtaccgaaa gattttgacg
     4861 gcgataccgc caaagtgctg attcgcggca aggaagcgga cgtgcgcgta ctgaagctgc
     4921 cgttcgtccg caacggtcaa aaacagtttg attaatatcg gttcagacgg cattttcatt
     4981 tcatatgccg tctgaaagca ggttttaatt gtcgtccgat acggacgttt gtagaaagca
     5041 ttgaacaagg catctgtgga tattgattca tgcagatgcc gtctgaaaat aaccccctat
     5101 caatggagta tcaaaccatg agcagcaaca tcccggcaga actgaaatac gttgccagcc
     5161 acgaatggct gcgccttgaa gaagacggta tcatcaccgt cggtattacc caccacgcgc
     5221 aagagctgtt gggcgacatc gtgtttgtcg agctgcctga agtcggcgca aaccttgccg
     5281 ccgaagaaca agccggtgtg gttgagtctg taaaagccgc gtccgacgtg tacgcaccga
     5341 ttgcaggtga agtcgttgcc gtcaacgaag atttgccaag cgctccggaa actgccaaca
     5401 gcgatcctta cggtgcaggc tggttcttca aactcaaacc ggcaaacgtt gccgattacg
     5461 acgttctgct gactgccgaa caatacgcgg gcgaagtgga ttaaaccgcc cggctgcccg
     5521 atggcaaccg ccggacaaac ggaaactgca ccttcagacg gcatttttgc ggtcggaggt
     5581 gcagtttttt gtccgtgttt taaggaagca gttaggctat aataacggtc tatattcatc
     5641 tttaccgatt ttttcatgca acttaccgct gtcggactca atcatcaaac cgcaccttta
     5701 agcatacggg aaaagctggc gtttgccgcg gcctgcctgc ccgaagccgt ccgcaatctt
     5761 gcccgaagca atgcggcaac ggaggcggta atcctttcta cctgcaaccg taccgagctt
     5821 tactgtgtag gtgattcgga agaaatcatc cgttggctcg cagactatca cagccttccc
     5881 atagaagaaa tcagccccta cctttatact ttggggatgc aggagactgt gcgccatgct
     5941 ttccgcgtcg cctgcggctt ggattcgatg gtgttgggcg agccgcagat tttaggacag
     6001 attaaggatg cggtcagggt tgctcaagag caggaaagta tgggtaagaa actcaatgcc
     6061 ctgttccaaa aaaccttttc tgttgctaaa gaggtccgta ccgatactgc cgtcggcgaa
     6121 aactcggttt ccatggcttc cgcttccgtc aagttggcag agcagatttt ccccgacatc
     6181 ggcgatttga atgtcttgtt tatcggtgcg ggtgagatga ttgagctggt tgccacttat
     6241 tttgccgcca aaagtccccg gctgatgacg gttgccaacc ggacgctggc gcgtgcacag
     6301 gagttgtgcg acaagctcgg tgtcaacgcc gaaccgtgcc tgctgtccga tctgcctgcc
     6361 attttgcatg agtacgacgt ggtggtttct tcaacggcaa gccagttgcc cattgtcggc
     6421 aaaggtatgg tggagcgcgc attgaaacaa aggcagagta tgccgttgtt tatgcttgac
     6481 ttggccgtgc cgcgagacat tgaggcggaa gtcggagatt tgaacgatgc ctatctttat
     6541 acggtggacg atatggtcaa tatcgtccaa agcggcaagg aggcaaggca gaaggccgcc
     6601 gccgccgccg aaacgctggt gtccgagaag gttgccgaat ttgtcaggca gcagcagggc
     6661 aggcagagtg tcccgttaat cagggcattg agggatgagg gagagaaagc gcgcaaacag
     6721 gtcttggaaa atgcgatgaa acagcttgcc aaaggcgcaa cggcagaaga ggttttggaa
     6781 aggctgtcga tccaactgac caacaagctg ctgcattcgc cgacccaaac cttgaataag
     6841 gcgggggaag aagataaaga tttggttcac gccgtcgcgc agatttatca tttggacaaa
     6901 taacggtgcg ccggaaaagg atgccgtctg aaggggtttc agacggcatt ttttgtgcag
     6961 gccggcaaca tcgtgaaatc ccacattata tcgatgtaat cacaaagtat agtggattaa
     7021 caaaaatcag gacaaggcga cgaagccgca gacagtacag atagtacggc aaggcgaggc
     7081 aacgctgtac tggtttaaat ttaatccact atattatccc gtatgcggat tggttttaag
     7141 atttgtaaat ttaatttgca tcaaaaaatc gccgatagat gattcatata atatcaatat
     7201 taaagagtat cggtatatcg gggatagtca tgtcctgttt ttcaatcaaa cgtatgtccg
     7261 cgtttcgggc gcggataaca gtgtttttta ccgcctttgt ctttttgacg gcggcactgc
     7321 ccgcttatgc ggagcgtctg cctgattttc tggcgaaaat acagccttcg gaaatttttc
     7381 cgggtgcgga ccgttacggc aagccggaag gcaagcctat ggttgcccgc gtttacaaag
     7441 gcgatgagca gttgggcttg gtctatatca cgaccgatgc ggtcaatacg cgcggttatt
     7501 cgaacaaacc gatcgatacg ctgatggctt tggcaaatga cggcacgata gccggagcga
     7561 aactggtcga tcatcacgaa ccgattatgc tgatcggtat cccgcatttg cccgcgccca
     7621 tgcgggcgat acgctcaaac tggcttccgg cgtatatcaa accaaacttt atatcgacaa
     7681 accgattacg attgaagggc ctgctgaccg ttctgcaacc atcgaaggag acaggagcgg
     7741 acgtacgata gctgtgcatg cgccggacgt aacgctccgc aacctgaccg ttacccgttc
     7801 cggtatgagc ctgcctgcaa tggatgccgg tatttatctc gaagaaaccg ccccgcgcgc
     7861 cctgattgaa cacaacaata ttttggataa ttcggtcggc gtatatctgc atggttctgc
     7921 cgatgcgatg gtgcgcgaga ataaaattgt cggcgacgcg actttgcgcg tgaacgagcg
     7981 cggcaacggc gttaccgttt ggaacgcacc cggcgcgcag gtcgtcggca acgacatttc
     8041 caaagggcgg gacggcattt tttccaatac cagcacgcac aacacctaca aaaacaaccg
     8101 cttcagcgat ttgcgtttcg ccgtccacta tatgtacacc aacgacagcg aaatcagcgg
     8161 caatatttcc gtgggcaaca atataggcta tgtgctgatg ttttccgagc ggctcaaagt
     8221 attcgacaat atcgccgtcg gcagccgcga tcagggcatt atgctcaact atgtcaacta
     8281 ttccgatatt cacgacaaca ttatcaacaa ggcaggcaag tgcgtatttg cctataatgc
     8341 caactacgat aaacttttcg ccaatcattt tgaaaactgc caaatcggca tacactttac
     8401 cgccgccatc gaaggcacgt ccctgcacga caattccttc atcaacaacg aaagccaggt
     8461 caaatacgtc agcacgcgct ttctcgactg gagcgagggc ggacacggca actattggag
     8521 cgacaacagc gcgttcgatt tgaacggcga cggcttcggc gacagcgcgt accggcccaa
     8581 cggcatcatc gaccaaatca tctggcgcgc gcccgtattg cgcctcttga tgaacagtct
     8641 cgcaatcagc atcgtcaaat gggcgcaggc gcagtttccc gccgttctgc ccggcggcgt
     8701 ggtggacagc aaaccgctga tgaagcctta tgccccccaa aattcaaacc cgttatcagg
     8761 cgatgaagga cgagttgctc aaagaagccg aaacgcggca gtcggaatgg ggcagggcgg
     8821 aaaacggttc tttgaactag tctgtttcag acggcatccg gattcaaatg ccgtctgaaa
     8881 acacaaaagg aaaaaccatg accacacatc atgtcgaatt gaggaaggta accaaacggt
     8941 tcggggcgca aaaagccgtc aaccaagtcg atttggtttt gaaggcagga gaaagcgtcg
     9001 ggcttgccgg acacaacggc gcgggcaagt ccaccattat gaagctgata ctcgggctga
     9061 ttaccccgac cgaaggcgaa gtgatgcttt tgggcgaacg taccggcagc aaagcggggg
     9121 cgcggcttcg cagccaaatc ggctacctgc ccgaaaccgt tgcgctgcac ccttcgctga
     9181 ccggcatcga aacgctggat ttttatgcca aacttaaaaa acagccgctc acgcagaacc
     9241 gggggctgct tgagcgcgtc ggcatttcac aggcggcaca ccgccgcgtc ggcacttatt
     9301 ctaaagggat gcgccaacgc cttgccttgg cacaagccct gctgggcgag cccaaagtcc
     9361 tgctgtttga cgaaccgaca accggcctcg accctgcatc aaggcaaatg ttttacgaag
     9421 tcgtgcgcga actcaacggg cgcggcgcga ccgtattgct cagcacccac gcccttgccg
     9481 agttggacgg acacgccgac cgcattatcg tggattaaat ttaatccact atatgcgggt
     9541 atggcgggtt tgagcggaca aatcagcctg accgtccccg ttttgctgac cgctcaggtt
     9601 ttatgggtta tcattccgct tgttttggca gccggaattt ttagaaagcg acaaatatga
     9661 aaaaaaccct gttggcaatt gttgccgttt ccgccttaag tgcctgccgg caggcggaag
     9721 aggggccgcc gcctttaccc cggcagatta gcgaccgttc ggtcggacac tattgcagta
     9781 tgaacctgac cgaacacaac ggccccaaag cccagatttt cttgaacggc aaacccgatc
     9841 agcccgtttg gttctccacc atcaagcaga tgttcggcta taccaagctg cccgaagagc
     9901 ctaaaggcat ccgcgtgatt tacgttaccg atatgggtaa tgttaccgat tggacaaatc
     9961 ccaatgccga cacgaagtgg atagatgcga aaaaagcctt ttatgtcatt gacagcggtt
    10021 ttatcggcgg tatgggtgcg gaagacgcac tgccgttcgg caacaaagag caggctgaga
    10081 aatttgcaaa ggataaagac ggtaaggttg tcagtttcga cgatatgcct gatacctata
    10141 ttttcaaata aatccaaaca aaaatgccgt ctgaacgttg aagccaaggc tcagacggca
    10201 ttttttgtca taggttcaaa cggggcaggt ttgaacaact gccccattcc ttattcctga
    10261 ccgggcgtaa cggcggaata aggttcacat cccgccgtaa ttcggcccgc ttgcgccttc
    10321 gggacaaatc caagtgatgt tttgcgtcgg gtctttgatg tcgcaggttt tgcagtgcac
    10381 gcagtttgcc gcgttgattt gcaggcgcgg attgccgttt tcttcaacaa tttcgtacac
    10441 gccggccgga caatagcgcg tttcgggcga ggcgtattct ttgtagttca cgtctatcat
    10501 cgtttgcgga ttgttcagca ccaaatggtc gggctggttt tcttcgtgcg cgagattggc
    10561 aaggaagacg ctgctcaagc ggtcgaaggt caacacgccg tcgggtttcg gataatcaat
    10621 cggcttacac gcggcggctt ttttaagctg ctcgttgtct ttgccgtgat gtttcaaggt
    10681 ccacggggct ttgcctctga aaatcatctg atcgatgccg gtgtagattg agccgaggta
    10741 aacgccccat ttgaatgacg gacggacatt gcgcgcggcg taaagctctt gatacagcca
    10801 gctttgttca aaacgttgct gataatccgc cgcctctttg ccgctgtcga aaccctccac
    10861 ttcttcaagg ttttccaaca aggggaacac ggcttcggcg gcgagcatgg cggatttcat
    10921 cgcggtatga atgcctttga tgcgcggcat attgaggaaa cccgccgcat cgccgaccaa
    10981 aatgccgcct ttgaacgaga gcttcggcaa actttgcaaa ccgccttcaa tcagcgaacg
    11041 cgcgccgtaa gcaatgcggc ggccgccttc aaaggttttg cggatttcgg gatgggtttt
    11101 gaaacgttgg aactcttcaa acggcgacag ataaggattt tgatagtcca aaccgaccac
    11161 gaagccgacg gcgactttgt tgtcgtcgaa atggtaaaca aacgcgccgc cgtaggtttt
    11221 gctgtccagc ggccagcctg cgctgtgcac caccaaaccg ggctgatgct gttcggacgg
    11281 cacttcccaa acttctttaa tgcccaagcc gtaagtttgc ggctggctgt tttggtcgag
    11341 ttggaaacgt tcgatgattt gtttggaaag cgaaccgcga caaccttcgg caaacagggt
    11401 ttgctgcgcc caaagctcca tgccgggttg gaatgaatcg gtcggctcgc cgtctttgcc
    11461 aatgcccata ttgccggttg caatgccttt gaccgaaccg tcttcgtgat acagcacttc
    11521 ggcggcggca aagcccggat agatttccac gcccatattt tccgcctgct ccgccaacca
    11581 gcgcacgact tcgcccaagc tgacgatgta gttgccgtga ttgtcgaaat tcggggtaat
    11641 cggcaggttg aacgcttttt tctcggtcag gaacaacact ttgtcctgcg ttactgtgcg
    11701 tgtcagcggt gcgccttttt ctttccagtc ggaaatcaac tcattcagcg caatcggatc
    11761 gataactgcg ccagccagcg aatgcgcccc cacctccgaa cctttctcca ccacgcaaac
    11821 gctgatttcg cgcccgtttt gttcggcaag ctgcttgagt ttgatggcgg cagacaaacc
    11881 cgacgggcct gcgccgacaa tcacgacatc gtattgcata ctgtcgcggg tgatggattc
    11941 tgtcatggcg gttcctgtgt atttattatt gaattgcaaa tccgtaatta tacaacggga
    12001 acatatagtt accaaataca acaaaggtcg tctgaaaacc atattttcgg ttttcagacg
    12061 acctttgtcg aaatttcaat aagcacgcca ccattttacc tgtccgaccg caaactccgt
    12121 ctgacgtttc ggactgcgtg tgaaaaacgc cttatccccg ccggcatccc tccctttcgg
    12181 cacaaccgcc aaaatcttac ctgccaaatt tccctcacgg gtttgccaag catccaaaaa
    12241 ctgcgccctg ctcatcgaaa catgacccag cgacgggtcg gcaagcaaaa ccgtattgcc
    12301 gtctataccg cgcaataccg agaaatgatc atccttgcgg tatttcagat acacgatgac
    12361 ggggatttgc aactgtgcaa gctgctcgaa agacagggca tagcctttcg cctcaaaacc
    12421 caaatcaggc atgatgcgcc gcatatcctc aaacgacgcg cgcatctgct ccttatccag
    12481 ctttttcaac acgtcctctt ccgtcagctt ttgcccgtga aaattgttca aaagccgtcg
    12541 ccaccgaagc cgccccgcag gaaaaatcca aatcctgctt tacaatattg aaatcgcgcc
    12601 tttctttcca actctgcact ttgatttttc cataagcaac aggattatag tggattaaat
    12661 ttaaaccagt acggcgttgc ctcgccttgc cgtactatct gtactgtctg cggtttcgtc
    12721 gccttgtcct gatttttgtt aatccactat aggtttccgt gcggacgtgt tcagattccc
    12781 gccttcgctg gaatgacggc ggagcgattt ctacttttcc gataaatgac cgtaacttaa
    12841 aatcccgtca tccccacgaa agcaaaaatc ccgcctgtcg gatttcggtt tttttgggcg
    12901 tttcgggaaa cttataaatc gtcattcccg cgcaggcggg aatccggttt gctcggtttc
    12961 ggtttttcgg gcgtttcggg aaactgatga atcgtcattc ccgcgcaggc gggaatctag
    13021 aacgcgggac ggcggcaata ttcaaaggtt gtctgaaaat tcagaggttc tagattccca
    13081 ctttcgtggg aatgacggga tataggtttc cctacggacg tgttcagatt cccgctttcg
    13141 cgggaatgac ggcggagcga tttctgcttt tccgataaat gaccgcaacc taaaacccat
    13201 ccttcccgca aaaacagaaa aacaaaaacc taaaatcccg tcatccccac gataacagtt
    13261 gcgtaattgc gtagagtggg cttcagccca acgttttttc tttttcggtc gttgattggt
    13321 gggctgaagc ccacccttgt atatcggaac tcccgtatca tagcaacaaa ccgcccggcc
    13381 gccacccgcg cccacccaag gcacacaacc gttgcgcagc acagggagcg gcagggcaac
    13441 ccatcgacac aaccggacag ttgccggaca acacaaccga atgcaaggca ggttgatgat
    13501 gagtacccga taccattacg caggtatagt gaattaaatc taaggggctg tactagatta
    13561 gccctaaatc ccacaggatt ttaagctgtt gagacggtgt gccgaagtta aatcgaaatt
    13621 cgcattcttt caagaacagc gggaaagatt tgcgatcgat tccattgtat tttcgcaaga
    13681 cgcgttttgc ctgattccaa aagttctcaa tgccgttaat gtggttctga cggtctgcac
    13741 actccttgga atggttgatg cggtaatgga taaaaccgct cacgtccaac ttgtcgtagc
    13801 tgctcagact atcggtataa acaatactat ccggcatgat tttctttttg atgacaggga
    13861 gtaacgtttc agacttggca ttatccacca caacggtata gacccgtccg ttgcgtttca
    13921 gaatgccgaa gacaaccact tttcctgccg caccgcgacc acgtctgcct ttacgccgtc
    13981 cgccgaaatc gctttcgtcc tgctcgacag ggccctcaaa aacctcatcg gcagccaaga
    14041 ccaaatgatg gttgataacc gtgcggattt tacggtagaa cagtgctgcc gaattgggat
    14101 ggatacccaa aatatcggcg gcagaacggg cggtaacttc gagtacaaaa aaacggagca
    14161 gttctttctg tacttttttc tttaatttgc agtgcgttat cttcatattt cgagggtaac
    14221 atatctgcta atctagtaca gccccaaaaa tataccaaaa acagcaaaac aaattgtaag
    14281 gatacgtata ggctttgtaa aggtaaattg tgaaaaaagc agttttttaa acgaatgaaa
    14341 cggcttcggg ctgaaatata tgctgatgcc ctgtccttcc cgtatatctt gtgtgttgtc
    14401 aaagtgcagg ctgctttgaa attggcatta cagtcgatga accaccattt tgctttattt
    14461 cagcgggctt gagatgtata taagaatatt gttttgaata aatttaaaga aaatgataat
    14521 cgttattgac gatttttaaa ggaaagcgta gagtgccaat tctatgaagc aatacggtaa
    14581 gtaacaatga aaatatctac tgcttgggta tagagcatat ttcacaaccc gtaactattc
    14641 ttgcggaaac agagaaaaaa gtttctcttc tatcttggat aaatatattt accctcagtt
    14701 tagttaagta ttggaattta tacctaagta gtaaaagtta gtaaattatt tttaa
//
LOCUS       DAWFLT010000051        14632 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWFLT010000051 DAWFLT010000000
VERSION     DAWFLT010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14632
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.051"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(32..922)
                     /locus_tag="WGR74_001428"
     CDS             complement(32..922)
                     /locus_tag="WGR74_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ0905828.1"
                     /translation="MFPVFHLSGESRRRMLQAALRFPHVFKARAEDSHKGTFGTLAVV
                     GGAVGMSGAPVLAASAAMYLGCGKVWAGFNQETLPFAVIAGFPEIMLDTADGLTKRQG
                     INAWTAGCGLGTDKAAVGTLAGILTEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITESM"
     gene            987..1643
                     /locus_tag="WGR74_001429"
     CDS             987..1643
                     /locus_tag="WGR74_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ0905829.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKTGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIREYMKNGIPEHVQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAVH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKAALAAALSVAALAAAAILLLS
                     "
     gene            1940..3400
                     /gene="cobA"
                     /locus_tag="WGR74_001430"
     CDS             1940..3400
                     /gene="cobA"
                     /locus_tag="WGR74_001430"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0905830.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKGKLKSVTERRRFWEKQFNGRFAALVKNRQNTLAERELAGQLEQSRQNDQGGS
                     VSLVGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGG
                     RTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAA
                     VGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALI
                     AERLQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLH
                     EKLAWFGENAKKESNPAEHAYFALDGLGTGQEQQAA"
     gene            3416..4150
                     /locus_tag="WGR74_001431"
     CDS             3416..4150
                     /locus_tag="WGR74_001431"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ0905831.1"
                     /translation="MKIFKPALWKIPPIENGSETALAEKTETLKQRLHRIAGSHRDAR
                     FASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHAETLNLLDRIERVYPHMQIKRFQP
                     IREDALHYVESKGRFAFYDSVEARRECCRIRKTEPLDRAIAGADAWLTGQRREQSATR
                     TELPFAEYDAGRGIDKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCTRP
                     VKAGEDIRAGRWWWEDKNSKECGLHK"
     gene            4188..5111
                     /gene="cysD"
                     /locus_tag="WGR74_001432"
     CDS             4188..5111
                     /gene="cysD"
                     /locus_tag="WGR74_001432"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ0905832.1"
                     /translation="MTKTEPNNVQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            5150..6436
                     /locus_tag="WGR74_001433"
     CDS             5150..6436
                     /locus_tag="WGR74_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ0905833.1"
                     /translation="MTAQHQTPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLDCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAGRLGLKDVHFVPVSALLGDNLVYPGGNMPWYRGEPLLAIL
                     ENLPGADETGRSAADFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            6433..8247
                     /locus_tag="WGR74_001434"
     CDS             6433..8247
                     /locus_tag="WGR74_001434"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ0905834.1"
                     /translation="MSEHDMQNTNPPLPPMPPEITQLLSGLNAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPTAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIQVSRAELKD
                     YKAKNIAGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDKRFEELGAKRLLERVDADLDFAAAANAWTDNIAALLKEEAAKNRATP
                     APQATPPAGLQTASEGRYCKAAPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARAGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFAADFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            8274..10043
                     /gene="cysI"
                     /locus_tag="WGR74_001435"
     CDS             8274..10043
                     /gene="cysI"
                     /locus_tag="WGR74_001435"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ0905835.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKTKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESALHQQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAVSDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPVRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVPEGGKARIEQLARSYGLIHDGVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            10216..12075
                     /gene="ilvD"
                     /locus_tag="WGR74_001436"
     CDS             10216..12075
                     /gene="ilvD"
                     /locus_tag="WGR74_001436"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ0905836.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNISNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHVGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDVTNPENTHAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIRLVISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(12138..12398)
                     /locus_tag="WGR74_001437"
     CDS             complement(12138..12398)
                     /locus_tag="WGR74_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ0905837.1"
                     /translation="MTKAVRRKNVQPPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            12721..13356
                     /locus_tag="WGR74_001438"
     CDS             12721..13356
                     /locus_tag="WGR74_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ0905838.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     KKLIAKTKQKAEIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGKFIS
                     GTIIPITQADDKIPKIDPEKTVIGRIIYLNRSDFPKGNGLDVSPDGSITRR"
     gene            complement(13434..14486)
                     /gene="bioB"
                     /locus_tag="WGR74_001439"
     CDS             complement(13434..14486)
                     /gene="bioB"
                     /locus_tag="WGR74_001439"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ0905839.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRQHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKVDY"
BASE COUNT         3449 a         4373 c         3946 g         2864 t
ORIGIN      
        1 gtactggttt ttgttaatcc actatatctt cttacatact ttcagttatc cggttgcgca
       61 gccaccttgc cgccggggcg atttcgccag ccaacagccc tgccgcaatg cctgccgatt
      121 cttttatgac atccgccgcc gcgccgtgca gccacgcgcc cgcgcaggcg gcttcaaaaa
      181 ccggcacgcc ctgtgcgagc agactgccga tgatgccgcc caatacgtcg ccactgcccg
      241 ccgttgccaa tcccgcgttg ccgctttcgt tgacatagat ttccgtatcg ggtgaggcaa
      301 ccaatgtttt gtgccccttt aaaaccacgg ttgcgccgaa aattgcccct atcttcctca
      361 ctgccgccgt ccgatccgcc tgaacctgtg caaccgtcgt tccaagcagg cgcgcggctt
      421 cggcggggtg tggcgttaaa atcaggtttt tacacccgcg tgccagattt cgggtttcgg
      481 catcggttga taatatgttc agcgcgtccg catccaaaag gacgggcttg tccgtgtgtt
      541 ccgtcaaaat tccggcaagc gttccgaccg ccgccttatc cgtacccaag ccgcaacccg
      601 ccgtccaagc gtttatgcct tgacgcttgg tcaaaccgtc tgccgtgtcc aacataattt
      661 cgggaaaacc ggcaatgacg gcaaacggaa gcgtttcctg attgaagccc gcccacactt
      721 tgccgcagcc gagatacatt gccgccgatg ccgccaatac gggcgcgccg ctcatgccga
      781 ccgcgccgcc gactacggcg agcgtgccga aagtcccttt gtgcgaatct tccgcacggg
      841 ctttgaaaac atgaggaaaa cgcaatgccg cctgaagcat ccggcggcgg ctttcgcctg
      901 aaagatggaa aacgggaaac atttcccctc caatcggtta aaattaaggc tcgaaaacaa
      961 accgattcta ccgccggaaa ccgattatga aaaacaaaac caaagtctgg gacttcccca
     1021 cccgcctttt ccactggctg cttgccgcat ccctaccctt tatgtggtat agcgcgaaaa
     1081 ccggcggcga tatgctgcaa tggcacacgc gcgtcgggct gtttatcctt ttcctgctcg
     1141 tattccgcct ctgctggggc atttggggca gcgataccgc ccgtttctcc cgtttcgtcc
     1201 gcggatggtc gggtatcaga gagtatatga aaaacggtat tcccgaacac gtccaacccg
     1261 gacacaaccc cttgggcgca ctgatggtcg ttgcgctttt ggccgccgtg tcgttccaag
     1321 tcggcacggg gctttttgcc gccgatgaaa acaccttcag caccaacggc tacctcaacc
     1381 atttggtttc cgaacatacg ggcagcctta tgcggaaaat ccatctcaac tttttcaaac
     1441 tgctcgccgt tttttccgca gtccacatcg ccgccgtcgc cgcataccgc gtgttcaaaa
     1501 agaaaaacct cgtcctcccg atgataaccg gcttcaaata catcgaaggc aaaacctcaa
     1561 tccgctttgc cggcaaagcc gcgcttgccg ccgcattatc ggttgccgcg cttgccgcag
     1621 ccgccatcct gctcctgtcc tgaaacccga catcaatgcc gtctgaagcc cttttcgccc
     1681 ttcggacggc attccccctc cgcgcaaacg gtctgaatgc ccgaaacggc ttcaggccgt
     1741 ttttccgtcc caaaccgtta taaaacggaa aaccgccgca tccgcagccg cccccatata
     1801 gctttcagct tctaacaaat gcctttttat tatttaaccg ggaaaatcct tttgtctaaa
     1861 atgacccgca ttccgacaaa aatgccgcga cgcaccgtat cggcaacaag gtgcgggcaa
     1921 acagcaaagg agtcagccag tgaattattt ccccattttc gccaacctcg ccggccgccc
     1981 cgtattggtc gtaggcggcg gcgcagtcgc cgcacgcaaa atcagcctgc tgctgaaggc
     2041 gggcgcagag gtcagggttg ccgcaaaaca cctgaatgcc gaactctccg cgttggcggc
     2101 ggaaaacaaa atcctgtggc ttgccgaaga atttcgtgcc gaacacatcc gcacggtttt
     2161 cctcatcatt gcggcaagca gcgaccaagc cctcaaccgg cgcgttttcc atcttgccga
     2221 aagctgccaa aagccggtca acgtggtgga tgaccgcgac cattgcagct tcatcttccc
     2281 atcggttatc gaccgcaacc cggttcagat tgccgtttcc agctccggca gcgcgcccgt
     2341 cctcgcccga ctgctgcgtg aaaggctgga agccctgctg ccgccgtctt tgggcgatat
     2401 ggcggaaatt tcaggaaggt ggcgcgatgc cgtcaagggc aaactgaaat ccgttaccga
     2461 acgccgccgc ttttgggaaa agcagtttaa cggacgtttc gccgccctcg tcaaaaaccg
     2521 gcaaaacacc cttgccgaac gggaattggc aggccaattg gaacaaagtc gtcaaaatga
     2581 tcaaggcggc tccgtctcgc tggtcggcgc gggtcccggc gatgcggggc tgctgacgct
     2641 caaagggctg caggaaatcc agcaggcaga cgtggtgctt tacgacgcgc tggtttcaga
     2701 cggcatactg tccctcgtcc gccgcgatgc ggaacggatt tttgtcggca agcgcgcccg
     2761 gggcggccgc accccgcaag aggacaccaa tgcgcttatg gtcaggctgg cccgcgaagg
     2821 caggcgcgtg gtgcgtttga aaggcggaga ccctttcgtg ttcggacgcg gcggcgaaga
     2881 attggaaacc ttggcacgcc atcagattcc gttttcggtc gtccccggca ttaccgccgc
     2941 cgtcggcgcg accgcctatg ccggcatccc gctcacgcac cgcgattatg cccaaagtgc
     3001 ggttttcgta accggccacc gcaaggcgga tgcgcccgac atcgaatggc agacccttgc
     3061 ccgcagccgc cagaccttgg tgatttatat gggtgcgctc aaagccgccc tgattgccga
     3121 acggctgcaa cagcacggac gctcgcccga cacgccggca gccgtcatca gccaaggcac
     3181 gctgccggct caaaaaaccg caaccggcac gctcgccaac cttgccgaac tcgccgaaac
     3241 cgcgccgaat ccggcattga ttgtcatagg cgaagtggtg gggctgcacg aaaaactggc
     3301 ttggttcggc gaaaacgcca aaaaagaaag caatccggcc gaacacgcgt attttgcatt
     3361 ggacggactg ggcaccggac aagaacaaca agccgcataa agcaaggcgg acaaaatgaa
     3421 aattttcaaa cctgctttat ggaaaattcc gccgattgaa aatggcagtg aaacggcttt
     3481 ggcggaaaaa acggaaaccc tcaaacaacg cctgcaccgg attgccggca gccaccggga
     3541 tgcacggttt gccagcagcc ttgctgcgga agatatggta attaccgacc ttatcgccgg
     3601 agaaaacctc aatatcggta tttttacttt ggatacgggg ctgctgcacg cggaaacttt
     3661 aaacctgttg gataggattg aacgtgttta tccgcatatg caaatcaagc gtttccaacc
     3721 gatacgggaa gatgccctgc attatgtgga aagcaaaggc aggtttgcct tttacgacag
     3781 cgttgaagcg cgccgcgaat gctgccgcat ccgaaaaacc gaaccgctcg accgcgccat
     3841 tgccggggcg gatgcttggc ttaccgggca acgccgcgaa cagtccgcca cacgcacgga
     3901 actgccgttt gccgaatatg atgccgggcg cggcatcgat aaatacaacc cgattttcga
     3961 ctggtcggaa cacgacgtgt gggcatacat ccttgccaac aatgtgcctt acaacgattt
     4021 gtaccggcaa ggctttccca gtataggctg cgacccctgc acccgccccg tcaaggccgg
     4081 cgaagacatc cgcgccgggc ggtggtggtg ggaagataaa aacagcaaag aatgcgggct
     4141 gcacaaataa ttcataagga tcaaacagaa gaaataagga atatggtatg acgaaaaccg
     4201 aaccgaacaa cgtccaactc gactggttgg aatccgaatc catccacatt atccgcgaag
     4261 tggcggcaga atgcgaaaac cccgccctgc tcttttccgg cggcaaagat tccgtggtcc
     4321 tgctcgccct cgcctgcaaa gccttccggc tgggcagccg gccggtgaaa ctgcccttcc
     4381 cgctggtgca tatcgacacg ggacacaact accccgaagt gattgctttc cgcgacgcgc
     4441 aggcggcaaa actcaatgcc cgcctgatag tggggcgcgt ggaagactcc attgccaaag
     4501 gcacggtggt cttacgcaaa gaaaccgatt cgcgcaatgc ggcacaggcg gttaccttgt
     4561 tggaaaccat cgaggcaaac ggcttcgacg cgcttatggg cggcgcgcgg cgcgacgaag
     4621 aaaaagcccg cgccaaagaa cggattttct cgttccgcga cgagttcgga caatgggatc
     4681 cgaaggcgca acgccccgag ctgtggtcgc tgtacaacac caggctgcac aaaggcgaaa
     4741 atatgcgcgt cttcccgatt tccaactgga cggaactcga catctggcaa tacatcgccc
     4801 gcgaaaacct cgagctgccg ccgatttatt acagccacag gcgcgaagtg gtcagacgca
     4861 gggggctgct ggtccccgta acgccgctca ccccgaaaat gccgtctgaa acctccgaaa
     4921 tccttgatgt ccgcttccgc accgtcggcg acatcagctg cacctgcccg gtagaaagca
     4981 ccgcgtccac gccgacggag attatcagag aaacagcggt tgccgacatt tccgaacgca
     5041 gcgcgacccg gctggacgat caggcaagcg aggcggcaat ggaaaaacgc aaaaaagaag
     5101 gctatttcta aatttcaaaa accctttaag cagatatgag gaagaaaata tgacggcaca
     5161 acaccaaacc ccgctccgct tcattaccgc cggcagcgtc gatgacggca aaagcaccct
     5221 aatcggacga ctgctctacg acagcaaagc cctgctgtcc gaccaaatca aaaccttgga
     5281 atccggcaaa agcaaaggtt tgaaagaaac catagacttc tccatcctga ccgacggact
     5341 ggaagccgaa cgcgaacagg gcattacgat cgatgtggca taccgctatt tttccacggc
     5401 gaaacggaaa ttcatcatcg ccgacacgcc ggggcacgaa caatacacgc gcaatatggt
     5461 tacgggcgca agcaccgctt cggcggcagt cgtgctggtt gacgcatccc aactggattt
     5521 cggcgcgcag cccttgcagc ttctgccgca gaccaaacgc cattccgcca tcctccgcca
     5581 actcgactgc ccgcatattg tggtggcggt caacaaaatg gacctgctgg attacagcga
     5641 agacaaattc aacgccattg tggaggcata ccgccggctc gccggacggc tcggtttgaa
     5701 agacgtgcat ttcgtaccgg tgtccgcact cttgggcgac aacctcgttt accccggcgg
     5761 caatatgccg tggtatcggg gcgaaccgct gctggccatc ttggaaaacc tgccgggggc
     5821 ggacgaaacc ggtcgttccg ctgccgattt ttatttcccc gtgcaactgg tcgtccgcca
     5881 agatgccgac aaggccgatg atttccgagg ctatcagggg cgcatcgaac gcggttcggt
     5941 cgccgtcggg caaaccgtcc gcatcgaacc gaacgggctg accgccgaag tgtccgaaat
     6001 catcacgccg aaaggggaag tggcgcaggc ttttgccggc gaggcggcaa ccctccggct
     6061 ggatcgcgac atcgatgttt cgcgcggcga cctttttgtc gataaaaatt ccccgctcgc
     6121 cccgcaaaaa catctggaag ccacgctttg ctggtttgac gaacgtccgc tcaacaccgc
     6181 gcgcaaatac ctgctcaagc acggcacgca aaccgtgccg gcaaaagtcg gggaaattga
     6241 aagcgttttg gatgtccgca cgctggaaca agaggccggc gcggaatcct tgaagatgaa
     6301 cgacatcgcc aaagtccgca tcaaccttca aaaacccgtt acggcaacgc cttatgcgga
     6361 aaacaccgcc gccggttcgt ttatcctgat agacgaagcg acatacggca ctgttgcggc
     6421 aggtatgatt ttatgagtga acacgatatg cagaacacaa atccgccatt accgcctatg
     6481 ccgcccgaaa tcacgcagct cctgtcgggg ctgaacgcgg cacaatgggc gtggctgtcc
     6541 ggctacgctt gggcaaaagc aggaaacggg gcatctgcag gactgcccgc gcttcagacg
     6601 gcattgccga cggcagaacc tttttccgta accgtccttt ccgcctcgca aaccggcaat
     6661 gcgaaatccg ttgccgacaa agcggcggac agcctggaag ccgccggcat ccaagtcagt
     6721 cgcgccgaac tgaaagacta taaggcgaaa aacatcgccg gcgaacgccg cctgctgctg
     6781 gttacgtcca cccaaggcga aggcgaaccg ccggaagaag ccgtcgtgct gcacaaactg
     6841 ctgaacggca aaaaagcccc gaaattggac aaactccaat ttgccgtact gggtttgggc
     6901 gacagttcct atccgaattt ctgccgggcg ggcaaagatt tcgacaaacg ttttgaagaa
     6961 ttgggggcga aacgcctgct cgaacgcgtt gatgcggatt tggactttgc cgccgccgca
     7021 aacgcctgga cagacaatat cgccgcactc ttaaaagaag aagccgcaaa aaaccgggca
     7081 acgcccgcgc cgcaggcaac gccccccgcc ggccttcaga cggcatcgga gggcaggtac
     7141 tgcaaggcag ccccctttcc cgccgccctg ctggccaatc agaaaattac cgcccgccaa
     7201 tccgataaag acgtgcgcca catcgaaatc gatttgagcg gttcggattt gcactaccta
     7261 ccgggcgacg cgctcggcgt ttggtttgac aacgatccgg cactggtcag ggaaatccta
     7321 gacctgctcg gcatcgatcc ggcaacggaa atacaggcgg gcggaaaaac cctgccggtt
     7381 gcctccgcac tgttatccca ttttgaactc acgcaaaaca cccccgcctt tgtcaaaggc
     7441 tatgccccgt tcgccgatga tgacgaactc gaccgtattg ctgccgacaa cgccgttttg
     7501 caaggctttg tgcaaagcac gccgattgcc gatgtgctgc accgcttccc ggcaaaactg
     7561 acggcggaac aattcgccgg cctactgcgc ccgcttgcgc cgcgcctgta ttcgatttcc
     7621 tcgtcgcagg cggaagtggg ggacgaagtg cacctgaccg tcggcgcggt gcgtttcgaa
     7681 cacgaagggc gcgccagggc gggcggcgca tcgggtttcc ttgccgaccg gctggaagag
     7741 gacggcacgg tgcgcgtgtt tgtggaacgc aacgacggct tcaggctgcc cgaagacagc
     7801 cgcaagccga ttgtgatgat cggctcgggc accggcgtcg caccgttccg cgccttcgtc
     7861 caacaacgtg ccgcagaaaa tgcggaaggc aaaaactggc tgattttcgg caatccgcat
     7921 tttgccgccg acttcctcta tcagaccgaa tggcagcagt ttgccaaaga cggcttcctg
     7981 cacaggtacg atttcgcctg gtcccgcgat caggaagaaa aaatctatgt gcaggacaaa
     8041 atccgcgaac aggcggaagg actttggcaa tggctgcagg aaggcgcgca tatctatgtg
     8101 tgcggcgatg cggcaaaaat ggcaaaagac gtggaagccg ccttgctgga tgtgattatc
     8161 ggggcaggac atttggacga agagggcgca gaagaatatt tggatatgct gcgcgaagaa
     8221 aaacgctatc agcgtgatgt ttattgatta aatataatcg ggaggaacac aaaatgaccg
     8281 tacagaccaa gacaaaaggt ttggcgtggc aagaaaaacc gctatccgac aacgaacgtc
     8341 tgaaaaccga aagcaatttt ttacgcggca cgattttgga cgatttgaaa gacccgctca
     8401 cgggcggctt caaaggcgac aacttccaac tcatccgctt ccacggtatg tatgagcagg
     8461 acgaccgcga catccgcgcc gaacgcgccg aggcaaaact cgagcccttg aaattcatgc
     8521 ttttgcgctg ccgactgccg ggcgggatca tcaaaccgtc ccaatggata gaactggaca
     8581 aatttgcccg ggaaaacagt cattaccgct ccatccggct gaccaaccgg caaaccttcc
     8641 aatttcacgg cgtgccgaaa accaagttgc agacgatgca ccgcctcttg cacaaactgg
     8701 gtttggattc catcgccacg gcggcggata tgaaccgcaa cgtgctttgc acctccaatc
     8761 ccatcgagtc ggcactccac caacaggctt acgaatacgc aaaaaaaatt tccgaacacc
     8821 tattgccacg cacgcgcggt tatctggatg tgtgggtgga cggcaaaaaa gttcaaagtt
     8881 ccgacgactt ccttcaagaa gacgagccga ttttgggcaa aacctatctg ccgcgaaaat
     8941 tcaaaaccgc agtcgtcatc ccgcccttga acgatgtgga ctgctacggc aacgatttgg
     9001 atttcgtcgc cgtttcagac ggtaacggac agcttgccgg cttcaatgtt ttggcaggcg
     9061 gcgggctttc gatggaacac ggcaacacca aaacctatcc gaacatttca ctggaactgg
     9121 gtttcgtgcc tccggaacac gcgctgaagg ccgccgaagc ggtggtaacc acgcagcgcg
     9181 acttcggcaa ccgcagcgac cgcaaaaacg cccgcacccg ctacaccatt caaaatatgg
     9241 gcttggacaa cttccgcgcc gaagtcgaac gccgtatggg tatgccgttc gaacccgtac
     9301 gcccgttcaa atttaccggg cgcggcgacc gtatcggctg ggtgaaaggc atagacggca
     9361 actggcattt gacccttttc atcgaaagcg ggcgtttggt tgacgaaggc gggaaacagc
     9421 ttctgaccgg cgtgttggaa atcgccaaaa tccacaaagg cgacttccgc atcaccgcca
     9481 accaaaacct catcgtggca aatgtacccg aaggaggcaa agcccggatc gaacagctcg
     9541 cccgctcata cgggctgata cacgacggtg tcagcaaact gcgcgaaaat gcgatgtcgt
     9601 gcgtttcctt cccgacctgc ccgctggcaa tggcggaagc cgaacgcgtg ctgccggact
     9661 tcatcggcga gctggataag attatggcga aacacggcac gtcggacgac tacatcgtta
     9721 cccgcattac cggctgcccg aacggctgcg gacgggcgat gttggcggaa atcggactgg
     9781 tcggcaaagc cgtcggacgc tacaacctcc atatcggcgg cgaccgtgaa ggcgtacgca
     9841 tcccccgtct ttacaaagaa aacatcaccc tgcccgaaat ccttgccgaa ttggacgacc
     9901 tgatcggcaa atgggcggca gaacgcaata tcggcgaagg tttcggcgat ttcgccatac
     9961 ggacgggcat cgtcaaaccc gtattaaatg cacccgttga tttttgggac gcatccaaag
    10021 ccgtcgcgat tgcccgcgcc tgaacccgtc cccgcattcc tgcggggatt ttcttttttg
    10081 ccgcacgccg tttcaatccc gcaaaattcc gatacttggt ataaaatacc ctcttttccc
    10141 actctaaaaa accgtacccg ataccgtctg aaacagccct gtctttcaga cggtataggc
    10201 acaaggaaac acactatgcc agaataccgc tccaaaacct ccacccacgg tcgcaatatg
    10261 gcgggcgcgc gcgcgttgtg gcgcgccacc ggcgtgatgg aaaccgactt cggcaaaccg
    10321 attatcgccg ttgccaactc gttcacccaa ttcgtgccag gtcatgtcca cctgcacaat
    10381 atgggccagc tggttgcccg cgaaatcgaa aaagccggcg ccatcgccaa agaattcaac
    10441 accatcgcca tcgacgacgg catcgctatg ggacacagcg gtatgctgta ttccctgccc
    10501 agccgcgatt tgattgccga ctccatcgaa tatatggtca atgcccactg cgccgacgcg
    10561 ctggtgtgca tttccaactg cgacaaaatc accccgggca tgctgattgc cgcgatgcgc
    10621 ctgaacatcc cgaccatctt cgtttccggc ggtccgatgg aagcaggtaa agttatcggc
    10681 gtggcaaaca tccagcccga acgccgtttg gacttgattg acgcgatgat tgaatcggcg
    10741 gacgacaata tcagcaaccg gcaagttgaa gaagtcgaac aaaacgcctg cccgacctgc
    10801 ggctcgtgtt cgggtatgtt tacggcaaac tcgatgaact gcctgaccga agcactcggg
    10861 ctgtccctgc cgggcaacgg ttcttacctc gccacccacg tcggccgcaa agaactgttc
    10921 ctcgaagccg gccgtatgat tgtcgaaatc accaaacgct attacgagca aaacgatgaa
    10981 accgtgttac cgcgcagcat tgccaccaaa aaagcgtttg aaaacgctat gaccatggac
    11041 attgccatgg gcggcagcac caacactatt ttgcatttac tcgccgttgc caacgaagcg
    11101 ggcgtggatt tcaaaatggc agacatcgac cgcttaagcc gcgttgtgcc ctgcatctgc
    11161 aaaaccgcac ccaacaacca cgactactat atggaagacg tgcaccgagc cggcggcatc
    11221 ttcgccatcc tgaaagaatt ggacaaagcg ggcaagctgc acaccgacgt gcacactatc
    11281 cacgcgccga cgctgaaaga cgcgattgaa aaatgggacg taaccaaccc tgaaaacacc
    11341 cacgccatcg aacgcttcaa agccgcgccc ggcggcgtac gcaccaccca agcgttctcg
    11401 caaaaccgta tgtggaaaac cctcgacctc gaccgcgaaa aaggctgtat ccgcgacgtg
    11461 gcacacgcct actcgcaaga cggcggtttg gcggtcttgt tcggcaacat cgccgagcgc
    11521 ggctgcgtgg taaaaaccgc aggcgtggac gagagcatcc tcaaattcac cggccgcgcc
    11581 cgcgtgtttg aaagccaaga agacgcagta gaaggcattt tgggcaacca aatcgtcgcc
    11641 ggcgacatcg tcatcatccg ctacgaaggc ccgaaaggcg gcccgggcat gcaggaaatg
    11701 ctgtatccga cttcctacct gaaatctaaa ggcctcggca aagcctgcgc tctcttaacc
    11761 gacggacgct tctccggcgg cacatcaggt ttgtccatcg gacacgcctc gcccgaagcg
    11821 gcggaaggtg gcgcgatcgg tttggtacac gaaggcgata ccgtcgaaat cgacatcccc
    11881 aaccgcagca tccgccttgt catttccgac gaagaactcg ccgcccgccg tgccgaaatg
    11941 gaagcacgcg gcagcaaagc gtggaaaccg gaaaaccgcg accgctacgt ctccgccgca
    12001 ttaagagctt acggcgcgat ggcgacttcc gccgacaaag gcgcggtgcg cgacgtagcg
    12061 caaatcgaaa gataagcccc aaaacgtgcg gcaaatgctg tctcgtctga acagtaaagg
    12121 tctgatgatg gacaaattta tctgggcgat ggcggtattt tccgcaattt tggcctcgtt
    12181 atcggcagcg gctttagcgc gatcgagtat gtaaaagagc tgccgctcgc cgccgttgcc
    12241 gtgatgcctt gcgcgccgaa gtcgttgctg tggttgttgg cgaagctgag gtagtcgaat
    12301 cccgcgtcgg caaggtattg cccgtatgcg gagggcgttc ggaatgcata gcatattttg
    12361 gggggttgca cattttttcg gcgtaccgcc ttcgtcaaac agcgtgcctt cgaggttgcc
    12421 gacggtaatg tccgcgtctt gcaaggcaga ttcgacgttt ttcagaatat tggtatcggg
    12481 caggtaatcg accggataat tgctgccgag cataatgtcg cccacgccga taatggaaac
    12541 ggtatcggct gtcttttcgt caggcgcatc ctgtccgtcc acatcgccgg tatttgaaac
    12601 gggcgcgggg tttgaaccgt caatcggcag cacggggatt ccgttccggc aggatgcgcc
    12661 gagggcttga ccccggcggt ttgcggtgcg gaaacggcat aaagggcgaa ccttgacgaa
    12721 ttgaacatca aatattcggg catcgaaaac aggtttgaaa ccgccatcct gaagaaaaac
    12781 ggcgtaagat ccggcttcgt ctccttcgcc cccaaccttg ccgccgtcaa actgaacgac
    12841 tatgccaaag tcaaaaaact gattgccaaa accaaacaaa aagccgagat cgttattgtg
    12901 atgttccacg gcggcgcgga agggaaacag gcggaacacc tgccgttcga taccgaaatc
    12961 ttctatgggg aaaacagggg caacgtcgtc gagtttgcac ggcttgccgt cgattccggc
    13021 gcggatgtcg tattcgggca ggggccgcac gttacacgcg ccgtcgaact ttaccgcgac
    13081 cgcttcatct cctacagcgg cggcaacttt gccacctacg gcgcaatcaa cacctccggc
    13141 atcagcggca ttgcgccgat tttcaaaatt atcaccgaca aacagggtaa atttatttcc
    13201 ggaactatca tccctattac ccaagccgac gataaaatcc ccaaaatcga ccccgagaaa
    13261 accgttatcg ggcggattat ttatctgaac cgcagcgatt tccccaaggg gaacgggctg
    13321 gatgtctcgc ccgacggcag catcacgcgc cggtaaaaag atattggaaa tgccgtccgg
    13381 acaaatgccg tctgaaagcc tttttccggg tttcagacgg catttttcaa tcgtcaataa
    13441 tccactttaa tcccagaggc tttttccact tcggcgacct cgccttccgg ttcaaactgc
    13501 aagggataca ggttgagctt ttccatcagg attctgtcgc cgtcctcatc aggattgccc
    13561 gtggtcagca gcttgtcgcc gtaaaaaatc gagttcgcgc ccgccataaa gcacatcgcc
    13621 tgcattgctt caggcatatt gctgcgccct gccgacagcc ggacataact ttgcggcatc
    13681 gtaatccgcg ccacggcgat ggtgcggaca aattccgtcc agtccaaatc ttcggcatcg
    13741 gcaagcggcg tgccttccac tttgaccaac cggttaatcg gcacgctttc gggctgcggg
    13801 tcgagattgg cgagactggc aatcagcccg gcacgttcgg cgcgggtttc gttcatcccg
    13861 acgatgccgc cgcagcagac tttcaaaccg gcgttgcgga ctttgcccaa ggtgtccatt
    13921 cggtcttcgt gttggcgggt gtggatgatg tcgttgtagc ggtcggggtc ggtgtcgagg
    13981 ttgtggttgt aataatccaa gcccgcctct ttcaagtctt ccgccatacc ttcttcgagc
    14041 ataccgaacg tgccgcaggt ttccataccc aagcccttga cggctttgat gattgcggaa
    14101 accgtctcca cgtctttggg tttagggccg cgccacgccg cgcccataca aaaccggctt
    14161 gcgccgcgcg atttggcgat tttggctttt tcgacgattt catccacatc catcatctgc
    14221 tctttgccca gattggtatt gtggtgcgcc gattgcggac aataggcgca gtcttcggga
    14281 caaccgccgg ttttgatgga caacagcgtg gaaagctgga tttcgcgcgg gttgaaattt
    14341 tggcggtgga tttcggcggc ttggtaaatg aggtcgagga agggaagtcc gaacagggct
    14401 tcgacatcgc attttttcca atagcgcgcg gtgggatgag gcttgcactc ggtcttacgg
    14461 cgcaaggcga cgggggatac ggtcataatg tgttctttcg tatttacagc ggcgcaatga
    14521 cgctgacagg caaaccgccg gcgcaatgac ggcggttgcg ggtaatcaaa tggctgaaaa
    14581 cagcggcggc aagtgtaacg cgtttgaaaa tacggggcaa acggtttttc cg
//
LOCUS       DAWFLT010000052        14177 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWFLT010000052 DAWFLT010000000
VERSION     DAWFLT010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14177
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.052"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..82)
                     /locus_tag="WGR74_001440"
     CDS             complement(<1..82)
                     /locus_tag="WGR74_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ0905840.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHII"
     gene            complement(83..1126)
                     /gene="galE"
                     /locus_tag="WGR74_001441"
                     /pseudo
     CDS             complement(83..1126)
                     /gene="galE"
                     /locus_tag="WGR74_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223275.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            complement(1119..2121)
                     /gene="dcm"
                     /locus_tag="WGR74_001442"
                     /pseudo
     CDS             complement(1119..2121)
                     /gene="dcm"
                     /locus_tag="WGR74_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(2152..4425)
                     /locus_tag="WGR74_001443"
     CDS             complement(2152..4425)
                     /locus_tag="WGR74_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ0905841.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSRLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKVSDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(4489..5139)
                     /locus_tag="WGR74_001444"
     CDS             complement(4489..5139)
                     /locus_tag="WGR74_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0905842.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(5136..5933)
                     /locus_tag="WGR74_001445"
     CDS             complement(5136..5933)
                     /locus_tag="WGR74_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0905843.1"
                     /translation="MKELHKTSFLESLLIQKRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLLTLVMVLMWKFFRMHNVSALNIVAFTLTGYPMMMMWRNASNHAIGSISANTSLL
                     YHRNVRVLDTIFARMLLEIAGATIAQVVIMFALVIIGWIDVPADIFYMLLAWLLMAMF
                     AVGLGLVICSVAFHFEPFGKVWSTISFVMMPLSGVFFFVHNLPQQLQHYVLMIPMVHG
                     TEMFRAGYFGDSVTTYENPWYILLCNLVLLLLGLAVVARFSKGVEPQ"
     gene            complement(5933..7096)
                     /locus_tag="WGR74_001446"
     CDS             complement(5933..7096)
                     /locus_tag="WGR74_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export inner-membrane
                     protein CtrB"
                     /protein_id="HEZ0905844.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVQEYMQSR
                     SALDALRKKMPIRDFYEKEGDIFSRFNGFGLRGEDEAFYQYYRDKVSIHFDSVSGISN
                     LSVTSFNAGESQKINDALLKQGEVLINQLNERARQDTIRYAQEVVNSAEEQVKEASVQ
                     LTKFRVSNGIFDLKAQSDVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLIARE
                     KSLRKEISQQMKAISGGGEGSLSNQAAEYQRVYLENELAEKQLAAAMTSLESAKAEAD
                     RQQLYLEVISQPNKPDLAYEPNRLYNIVATFVIGLIVYGIVVLLSASIREHKN"
     gene            complement(7111..8274)
                     /gene="ctrA"
                     /locus_tag="WGR74_001447"
     CDS             complement(7111..8274)
                     /gene="ctrA"
                     /locus_tag="WGR74_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ0905845.1"
                     /translation="MLKLKFCIVISFLILGSACSAIPSSGPSAKKVVSLGQQSEAQIP
                     EVELIDVNHAVAQSLYKAQVNQSFTQFGDGYASTGTLNIGDVLDIMIWEAPPAVLFGG
                     GLSSMGSGSAQQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKKMANQ
                     PQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNVQLTR
                     GNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSARGLSL
                     SEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVYRVNM
                     ADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            8493..9611
                     /gene="wecB"
                     /locus_tag="WGR74_001448"
     CDS             8493..9611
                     /gene="wecB"
                     /locus_tag="WGR74_001448"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEZ0905846.1"
                     /translation="MKVLTVFGTRPEAIKMAPVILELQKHNTITSKVCITAQHREMLD
                     QVLSLFEIKADYDLNIMKPNQSLQEITTNIISSLTDVLEDFKPDCVLVHGDTTTTFAA
                     SLAAFYQKIPVGHIEAGLRTYNLYSPWPEEANRRLTSVLSQWHFAPTEDSKNNLLSES
                     IPSDKVIVTGNTVIDALMVSLEKLKITTIKKQMEQAFPFIQDNSKVILITAHRRENHG
                     EGIKNIGLSILELAKKYPTFSFVIPLHLNPNVRKPIQDLLSSVHNVHLIEPQEYLPFV
                     YLMSKSHIILSDSGGIQEEAPSLGKPVLVLRDTTERPEAVAAGTVKLVGSETQNIIES
                     FTQLIEYPEYYEKMANIENPYGIGNASKIIVETLLKNR"
     gene            9613..11250
                     /locus_tag="WGR74_001449"
     CDS             9613..11250
                     /locus_tag="WGR74_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide phosphotransferase SacB"
                     /protein_id="HEZ0905847.1"
                     /translation="MFILNNRKWRKLKRDPSAFFRDSKFNFLRYFSAKKFAKNFKNSS
                     HIHKTNISKAQSNISSTLKQNRKQDMLIPINFFNFEYIVKKLNNQNAIGVYILPSNLT
                     LKPALCILESHKEDFLNKFLLTISSENLKLQYKFNGQIKNPKSVNEIWTDLFSIAHVD
                     MKLSTDRTLSSSISQFWFRLEFCKEDKDFILFPTANRYSRKLWKHSIKNNQLFKEGIR
                     NYSEISSLPYEEDHNFDIDLVFTWVNSEDKNWQELYKKYKPDFNSDATSTSRFLSRDE
                     LKFALRSWEMNGSFIRKIFIVSNCAPPAWLDLNNPKIQWVYHEEIMPQSALPTFSSHA
                     IETSLHHIPGISNYFIYSNDDFLLTKPLNKDNFFYSNGIAKLRLEAWGNVNGECTEGE
                     PDYLNGARNANTLLEKEFKKFTTKLHTHSPQSMRTDILFEMEKKYPEEFNRTLHNKFR
                     SLDDIAVTGYLYHHYALLSGRALQSSDKTELVQQNHDFKKKLNNVVTLTKERNFDKLP
                     LSVCINDGADSHLNEEWNVQVIKFLETLFPLPSSFEK"
     gene            11323..12066
                     /locus_tag="WGR74_001450"
     CDS             11323..12066
                     /locus_tag="WGR74_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEZ0905848.1"
                     /translation="MLSNLKTGNNILGLPEFELNGCRFLYKKGIEKTIITFSAFPPKD
                     IAQKYNYIKDFLSSNYTFLAFLDTKYPEDDARGTYYITNELDNGYLQTIHCIIQLLSN
                     TNQEDTYLLGSSKGGVGALLLGLTYNYPNIIINAPQAKLADYIKTRSKTILSYMLGTS
                     KRFQDINYDYINDFLLSKIKTCDSSLKWNIHITCGKDDSYHLNELEILKNEFNIKAIT
                     IKTKLISGGHDNEAIAHYREYFKTIIQNI"
     gene            12068..12931
                     /locus_tag="WGR74_001451"
     CDS             12068..12931
                     /locus_tag="WGR74_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEZ0905849.1"
                     /translation="MRKITFIIPIKQSLIKPDCFIRLFFNLFLLKKFSSKYGFSILVA
                     DNSNFLWKNIIKLITKFYKCNYISIKSHNTFYTPAKIKNAAAIYSFNTLNSNYILFLD
                     VDVLLSENFIQHLIKKTKTNIAFDWYPVSFLNKQFGIINFILFSYKGNLNIEESFIIQ
                     TGFVTGLQLFNSDFFYKTAGYNESFLGYGCEDIEMIHRATLLLNIRPAFNENHQYFTD
                     DRGYMPSKLTGFRNYFYYLKRDEFSNLQITPKHFWHKRKNKSKYLKNRYQNDVKMIQI
                     MKDFDRKFLKN"
     gene            12976..13995
                     /gene="galE"
                     /locus_tag="WGR74_001452"
     CDS             12976..13995
                     /gene="galE"
                     /locus_tag="WGR74_001452"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ0905850.1"
                     /translation="MKNILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPLGDTTSPYGTSKSMVERI
                     LTDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAV
                     FGDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRA
                     FEAASGLTIPYEVKPRRAGDLACFYADPSYTKAQIGWQTQRDLTQMMEDSWRWVSNNP
                     NGYDD"
     gene            14111..>14177
                     /locus_tag="WGR74_001453"
     CDS             14111..>14177
                     /locus_tag="WGR74_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEZ0905851.1"
                     /translation="MRKILVTGGAGFIGSAVVRHII"
BASE COUNT         4184 a         3205 c         2846 g         3942 t
ORIGIN      
        1 gaatgatatg gcggacaacg gcagagccga taaagcccgc gccgccggta acgaggatgg
       61 tttttttgtt tgcggtttgc atttaatccc catatccgtt ggggttgcgg ctgacccaac
      121 gccacgaatc ctccatcatt tgcgcaaggc tgcgtttggt ttcccaacct gtctcttgtt
      181 tggtatggga cgggtcggca aaggaacacg ccaagtctcc ggcgcgtcgg ggttggattt
      241 gatagggaat atttaagccg gatgcggctt caaaggcacg gatgatttcc aaaaccgaat
      301 aggcgcgccc cgaacccaag ttgaacaaat gtacgccggc aacaccgcct ttcgccttca
      361 ttgccgcgat atgcccttct gccaaatcca tcacatgaat atagtcgcgc attcccgtac
      421 cgtccggggt cggataatcg ccgccgaata ccgacagttg cggcagcctg cccgaagcca
      481 cttggcagat atagggcaaa aggttgttgg gaataccgtt gggctgttca ccgatcaagc
      541 cgctttcgtg cgcgccgatc gggttgaaat agcgcaacaa aatcacgctc caacgcggat
      601 cggccttttg gatgtcggtt aacatccgtt ccaccatcgc tttggatgcg ccataaggat
      661 tagcggtatc gcccgggcgc atatcttccg tatagggcat tttacgtatc atggcgcgac
      721 gcattaaagt aaacgccaaa gcgcgtttat actggttatc cagtttccct atatttttac
      781 gttacaaatc aagttgttgg tattcatcgt gaaaatagaa tttttcggca taacgctgac
      841 tcataaaacc ggcatgcggg ctgcctgaaa aaatcattgc gacatcatcg tcatttaatg
      901 tttcatggtt gttttctatt aatgctaaag caatttggta attaattgcc aaaatatcgt
      961 tagtaatcac acgatagccg cgttttttgg cttcgtaggc aaaggaacag cctccactaa
     1021 atacatctgc aactgtatct acatcagacg gaagctggtc acaaatccag gaagctattt
     1081 tctctttatt accgatatag ttaattttcg gatattgctt attcattttc actcattttt
     1141 aaaatacttt cagcaatagc ttttgccaat aaaggcggta cggcgttacc aacctgctgc
     1201 tgctgggcaa ttttgctgcc gcaaaaaata aaattatcag ggaaagattg taaggcagct
     1261 aattcacgaa cggttaacgc ccgattctgt tcatagtgaa aaactttgcg catatctcct
     1321 gtaatacaaa cggctggttt tgttgctgtt gtaacggatg tatttacgga tatcacctgt
     1381 tttcggacgt aatggttcag gaatatcgtt acggttacct ccatttttaa caaatgccat
     1441 tttttctaac atttgtgccg aatgattcat agcttcatga tttgcaacgt gtggattgct
     1501 ttcgccagca gccagttttg gaaaatgtcc tattgctgat ccaacagtct gatgggaaat
     1561 ctgcaaaggt tcgggaaagg aaattttgcc tttatccctc ctcccgataa atatcactcg
     1621 gctacgtatc tgaggaacac cgaaatcggc tgcactcagt atcttacatt ccaccgaata
     1681 accgatattc tgaaatgctt gaataatctc aatacgtgtt ttacctgaat tgtgtgtata
     1741 gagtcgcgct acattttcca taacaaaaaa atatggttgg acaattttaa ctattcggac
     1801 aaactcttta aataaatggt tgcgtgggtc atctgtaaat gtccgtccaa tctttcctgc
     1861 catactaaaa ccttgacaag gtggtcctcc aataatcaaa tcaactgctt gtccgttaag
     1921 acaattgatt aaatcttgtt cggttagtgt ggttaaatct ttttgcagta attgatgatg
     1981 ggggaagttg gtacggtaag tctgacaata atcagactcc atttcaacag aaagcaattg
     2041 ttggaatccg gcttgttcaa aacccaagga taggcctcct gctcctgaga aaaggtcaat
     2101 ataggtaagt ggtgtgtgca taagtcaaaa tccataaact cttcgattat tttaccgctt
     2161 cagcttcgca aacgcatccg ccatcgccga attggctggg gcgcggtcgt tgcgttgggg
     2221 tttgcggccg gctgtccgtt cgcggctgcg gttttcagac ggcattttat gttttgcgcc
     2281 gcccggttcg tcatccaagc gcatggtcag cgcgatgcgt ttgcgtgcag catcgacttc
     2341 cagcactttc actttcacca cgtcgccagc tttcaccact tcgcgcgggt cttggacgaa
     2401 cttgttggac agggcggaga tgtgcaccaa gccgtcctga tggacgccga tgtccacgaa
     2461 cgcgccgaag ttggcgacgt tggaaaccac gccttcgagt atcataccga cttgcaagtc
     2521 gctgatttcg tggatacctt cggcaaacga tgccgtctga aactcgccgc gcggatcacg
     2581 gccgggtttt tccagttcgg acaggatgtc caaaatggtc ggcaggccga agcgttcgtc
     2641 ggtgaagtcg gacactttga tttgcttcac gcgctcgcgg ttgccgatga gttcggcggc
     2701 gctaatgcct tgttgcgcca gcattttggc gacgacggga taggcttcgg ggtggacggc
     2761 gctcgcgtcc aacggctctt taccgccgtt aatccgcaaa aagcctgccg cctgctcgaa
     2821 ggttttttcg cccaaacgcg gcactttcag caattttttg cggctgtcga acgcgccgtt
     2881 ttcatcgcgg taggcgacga tgttttgggc aagagtttga ttcaaaccag aaatccgcgc
     2941 caagagcggg gcggaggcgg tattcacgtc cacgccgacg gcgttcacgc agtcttcgac
     3001 caccgcgtcc agcgatttgg cgagccggct ttggttcacg tcgtgctgat actggcccac
     3061 gccgatggat ttggggtcga ttttgaccag ctcggcaagc gggtcttgca gcctgcgggc
     3121 gatggacacc gcgccgcgca gggaaacgtc caagtcgggg aactcgcgcg ctgccagttc
     3181 ggacgcggaa taaatcgacg cgccggcttc ggacacgacg attttatgca gccccatttt
     3241 cgacatttca cgcaccagtt cgcccgcgat tttgtcggtt tcacggctgg cggtgccgtt
     3301 accgatggcg atgagcttca cgccgtgctg tttaatcagg cgcgacagcg ttgccaacat
     3361 attgttttct tgatgcaaat agacgatgac ggtatccagc agcttgccgg tgtcgtccac
     3421 cacggcgcat ttcacgccgt tgcggtagcc ggggtcgaga cccagcgtgg tcagccgtcc
     3481 ggcgggcgca gcaagcagca agtctttgag attgcgggcg aacacggtaa tcgcgtcggt
     3541 gtcggcggct tctttcaaac gattgagagc ttcaagttcc aacgacaaaa agattttcgc
     3601 gcgccaagtc aggcgcacgg tgtcgcgcag ccatttgtgg ccgtctgaaa ctttgaagcg
     3661 gcgggcgatg atttgctcgt attcgctttg ctgcgtaatc ggcgtgtcgt cgggctgata
     3721 tttgagcgcg atgttcaaca cgccttcgtt gcgtccgcgc aaaaccgcta gcgcacgatg
     3781 gctgggcatc gcacgaatag gttcgcggtg gtcaaaataa tcgctgaatt tttcgccttc
     3841 ggtttctttg cctccaacaa cttgcgcgtg gatttcggct tcgttccaca gcttgtcgcg
     3901 cagcgtgccg atgagttccg cgtcttcggc aaactgctcc atcagaatcg cacgcgcgcc
     3961 gtccaacgcg gctttggcat cggggacgtt ttcgttcagg tagccttgtg cggcggcttc
     4021 cacgtcctgc gactgctcgg caagcaacac gtccgccagc ggctgcaaac cgtgttcgcg
     4081 cgcgatttgc gctttggtgc ggcgtttggg tttgtagggc agatacaggt cttccagcgc
     4141 ggttttgtta tcggcggctt cgatttgcgc cctgaggtcg tctgaaagct tgccttgctc
     4201 ttcaatgctt tttaaaacaa cggctttgcg ctcttccaac tcgcgcagat attgcagccg
     4261 ctcggcaagc cggcgcaact gcgtatcgtc caacccgccc gtcgcttcct tgcggtagcg
     4321 ggcgataaac ggcacggtcg cgccgtcgtc caaaagctcg acggcggcgg tgatttgcgc
     4381 ggcagtcgcg gagagttctt gggaaagaat ttgagtaatg ttcatcaata aaattccaac
     4441 ggacaggccg tctgaaattt cagacggcct gatttaaaaa caatcgcttt aaggcagcga
     4501 attataatat tcgtaggctt tgtccatatc ttcaaactgg tacatatgcc ctttttccag
     4561 caccatcgca ttatcgcaat attgcttcat ggcgctgtgg ctgtgcgaca ccaagatgat
     4621 ggaacggtct ttgcgctttt caaacaactc gtacttacat ttatcggcaa aacgcgagtc
     4681 accaactgca atcacttcgt caatcaaata gcaatcaaac tctaccgcca acgacaaagc
     4741 aaatgccaaa cgcgccttca tacctgaaga ataacgcttg accggctcgt aaagatattg
     4801 ccccaactcg gaaaactctt cggtaaagtt cttcacataa tcgatatcga cattgtaaat
     4861 ccggcagatg aaacgcaaat tgtccatacc ggtcagactg ccttgaaacg caccggagaa
     4921 tgccaaaggc caagaaatac tcattgtccg cttgatttca cccgtggtcg gcggctcaac
     4981 gccactgatc aaacggatga gcgtcgattt acctgcaccg ttgcggccga gaataccgat
     5041 tttctcgccc ttctccattt tgaagctaat atcgtgcaag actgtccgcc aaccttggcg
     5101 ggtcagatag cgtttggaaa cgtgttcaac tgaaatcatt gcggctcaac ccctttgctg
     5161 aatctcgcca ccaccgccaa accaagcagc agcaaaacca agttacacaa caaaatatac
     5221 caaggatttt cgtaagtcgt gacgctgtcg ccaaaatatc cggcacggaa catttctgta
     5281 ccatgcacca tcggaatcat cagcacatag tgttgcaact gctgaggcag gttgtgtacg
     5341 aagaaaaaca cgccggacaa aggcatcatc acaaaactga tggtactcca aactttgcca
     5401 aacggttcaa aatgaaacgc caccgaacag atgaccagcc ccaatccaac ggcaaacata
     5461 gccatcaaaa gccaagccaa caacatatag aaaatatctg ccggtacatc aatccaaccg
     5521 atgataacca atgcgaacat aatcacgacc tgcgcaatgg tggcgccggc aatctccaac
     5581 aacatacgcg caaaaatggt atccaatacg cgtacatttc ggtgatacag caggcttgtg
     5641 ttggcagaaa tcgaaccgat agcatgatta gaagcattac gccacatcat catcatcgga
     5701 tagcctgtca gcgtgaatgc cacgatattc aaagctgaca cgttgtgcat acggaaaaac
     5761 ttccacatca acaccataac caaagtcagc agcaaaggtt cgacaaacaa ccacaaaaaa
     5821 ccaatgttat tacgaccgta gcgagtaata atttcacgca ttaagagtgc gccgattacc
     5881 cttttttgga taagcagcga ctccaaaaac gatgttttat gtagctcttt catcagtttt
     5941 tatgctcacg gatacttgca gacagcaaca cgacaatgcc atagacaatc aaaccgatga
     6001 caaacgttgc cacgatgttg tacaaacggt ttggctcata tgccaagtca ggtttgttcg
     6061 gctgggaaat cacttccaaa tacaattgct gacgatctgc ttccgctttt gcactttcca
     6121 aagaagtcat cgccgcagcc aattgttttt cggccaattc gttttccaaa tacacgcgtt
     6181 gatattccgc cgcctgattg gacaacgaac cctcgccacc gccggaaatc gctttcattt
     6241 gttgcgagat ttctttacgc aaactcttct cacgcgcaat caaacctgga atttgcgggt
     6301 tttcaggcgt aacggctttt acctggtcca gctgggtctg aatgacaatc aactcgtcct
     6361 gtaatttgga taccaaaccc atttgcacat cggattgcgc cttcaagtcg aaaataccgt
     6421 tagacacacg gaattttgtc aattggacag aagcctcttt aacttgctct tcagcagaat
     6481 tgaccacttc ctgagcataa cgaatcgtat cctgacgcgc acgttcgttc agctggttaa
     6541 tcaatacctc accctgttta agcaaagcat cattgatctt ttgagattca ccggcattaa
     6601 acgatgtaac gctcaaattg gaaatgcctg agacagagtc aaaatggatg gataccttat
     6661 cacggtagta ttgataaaac gcctcatcct cgccacgcag gccaaaacca ttaaaacggc
     6721 tgaaaatatc gccttctttt tcataaaaat cgcgaatggg cattttctta cgcaacgcat
     6781 ccaaagccga acgcgactgc atatattcct gcacggtata aatatcgtct tgcgcacggg
     6841 caaaacctgt gccctgcaaa atggcaccca ggccattgag agaagattgg cttttaggcg
     6901 agcgcaccac aaagctcgat tgcgacgtaa aacgatcgga agcgaagaag ccgaaataca
     6961 ccaacgaaat taccgtaggg ataatcaccg ttacccaaaa taaagggctt agctttttaa
     7021 tccaactttt ctttttcggc tttttacgct cggctttggt ttcagtggca actgccacag
     7081 gaagttgctc agacatctta attactcaca ttaattagtt aaattattaa tactgttcgc
     7141 gccactggta accggcgaga acacaaacga caagaatttc tgcacttcag ccaacggcgc
     7201 attcgacaca tacaatacat ctttattctt cacaggaaag cgctgcatag aaaatagcga
     7261 atgcgcatca gccatattca cacgatatac cgttggaatc tctgcctcac tgccataacc
     7321 ttgagcaatc catttatcct gacgttctgc cggcaattcc accaatggcg tatagcggaa
     7381 cacaaacaca ccacgcgcat cagaacggcg atcttgcaaa ccgcccatac ggccaatggc
     7441 ttcagaaagc gataagcctc tggctgaaaa accgatttct tgtgttctcc ccaccgcacc
     7501 catagacgta aaggtatagg gattggtaat catggtaacc acatcaccgc gacgcagcaa
     7561 aatattttgt cgcggatttg caactaaatc ttccaaggca acagttcgta ctacattgcc
     7621 acgtgtcagc tgcacattcg tatcctgcac atttgccgtt gaaccaccta ccgcagccac
     7681 cgcatccaac acacgctcac cggctgccgt caacggcata cgcacactat tgcctgcgcg
     7741 aatcaccgat acatttgccg cattattctg caccaagcgc accatcactt gcggctgatt
     7801 ggccattttt ttcaggcggc ctttaataat ttcctgaacc tgaccaggcg ttttaccgac
     7861 caccgaaata tcgccaacaa acggcacaga aaccgtacca cgtgccgtca ccagttgctc
     7921 cggcaacttg gtttgttgcg cactacccga gcccatcgaa gaaaggccac caccaaacaa
     7981 taccgctggc ggcgcttccc aaatcataat atccaataca tcaccaatat ttagcgtacc
     8041 ggtagaagca taaccatcgc caaactgggt aaaagactga ttcacctgag ctttatacaa
     8101 agactgcgct acagcatgat tgacgtcaat caactctact tcaggaattt gagcttcaga
     8161 ctgttgcccc aatgagacaa ctttttttgc acttgggcca gaggagggaa tagcactaca
     8221 tgcacttccc aaaattaaaa aagaaattac aatacaaaac tttaacttaa gcataaaata
     8281 aaaaatctca ttaagtatga ttgtttttaa ataaatttaa aacctaccag agatacaata
     8341 ccactttatt ttgtagaaca caaacgtgta taatatataa cataaacatc atcttcgaaa
     8401 taatattggg gcttaggaag caaaatcatc aaaaaacgtg ataagctcct aatattttta
     8461 acacattact atattacaca taggatattc caatgaaagt cttaaccgtc tttggcactc
     8521 gccctgaagc tattaaaatg gcgcctgtaa ttctagagtt acaaaaacat aacacaatta
     8581 cttcaaaagt ttgcattact gcacagcatc gtgaaatgct agatcaggtt ttgagcctat
     8641 tcgaaatcaa agctgattat gatttaaata tcatgaaacc caaccagagc ctacaagaaa
     8701 tcacaacaaa tatcatctca agccttaccg atgttcttga agatttcaaa cctgactgcg
     8761 tccttgttca cggagacacc acaacaactt ttgcagctag ccttgctgca ttctatcaaa
     8821 aaatacctgt tggccacatt gaagcaggcc tgagaactta taatttatac tctccttggc
     8881 cagaggaagc aaataggcgt ttaacaagcg ttctaagcca gtggcatttt gcacctactg
     8941 aagattctaa aaataactta ctatctgaat caataccttc tgacaaagtt attgttactg
     9001 gaaatactgt catagatgca ctaatggtat ctctagaaaa actaaaaata actacaatta
     9061 aaaaacaaat ggaacaagct tttccattta ttcaggacaa ctctaaagta attttaatta
     9121 ccgctcatag aagagaaaat catggggaag gtattaaaaa tattggactt tctatcttag
     9181 aattagctaa aaaataccca acattctctt ttgtgattcc gctccattta aatcctaacg
     9241 ttagaaaacc aattcaagat ttattatcct ctgtgcacaa tgttcatctt attgagccac
     9301 aagaatactt accattcgta tatttaatgt ctaaaagcca tataatatta agtgattcag
     9361 gcggcataca agaagaagct ccatccctag gaaaaccagt tcttgtatta agagatacta
     9421 cagaacgtcc tgaagctgta gctgcaggaa ctgtaaaatt agtaggttct gaaactcaaa
     9481 atattattga gagctttaca caactaattg aataccctga atattatgaa aaaatggcta
     9541 atattgaaaa cccttacggg ataggtaatg cctcaaaaat cattgtagaa actttattaa
     9601 agaatagata aaatgtttat acttaataac agaaaatggc gtaaacttaa aagagaccct
     9661 agcgctttct ttcgagatag taaatttaac tttttaagat atttttctgc taaaaaattt
     9721 gcaaagaatt ttaaaaattc atcacatatc cataaaacta atataagtaa agctcaatca
     9781 aatatttctt caaccttaaa acaaaatcgg aaacaagata tgttaattcc tattaatttt
     9841 tttaattttg aatatatagt taaaaaactt aacaatcaaa acgcaatagg tgtatatatt
     9901 cttccttcta atcttactct taagcctgca ttatgtattc tagaatcaca taaagaagac
     9961 tttttaaata aatttcttct tactatttcc tctgaaaatt taaagcttca atacaaattt
    10021 aatggacaaa taaaaaatcc taagtccgta aatgaaattt ggacagattt atttagcatt
    10081 gctcatgttg acatgaaact cagcacagat agaactttaa gttcatctat atctcaattt
    10141 tggttcagat tagagttctg taaagaagat aaggatttta tcttatttcc tacagctaac
    10201 agatattcta gaaaactttg gaagcactct attaaaaata atcaattatt taaagaaggc
    10261 atacgaaact attcagaaat atcttcatta ccctatgaag aagatcataa ttttgatatt
    10321 gatttagtat ttacttgggt caactcagaa gataagaatt ggcaagagtt atataaaaaa
    10381 tataagcccg actttaatag cgatgcaacc agtacatcga gattccttag tagagatgaa
    10441 ttaaaattcg cattacgctc ttgggaaatg aatggatcct tcattcgaaa aatttttatt
    10501 gtctctaatt gtgctccccc agcatggcta gatttaaata accctaaaat tcaatgggta
    10561 tatcacgaag aaattatgcc acaaagtgcc cttcctactt ttagctcaca tgctattgaa
    10621 accagcttgc accatatacc aggaattagt aactatttta tttacagcaa tgacgacttc
    10681 ctattaacta aaccattgaa taaagacaat ttcttctatt cgaatggtat tgcaaagtta
    10741 agattagaag catggggaaa tgttaatggt gaatgtactg aaggagaacc tgactactta
    10801 aatggtgctc gcaatgcgaa cactctctta gaaaaggaat ttaaaaaatt tactactaaa
    10861 ctacatactc actcccctca atccatgaga actgatattt tatttgagat ggaaaaaaaa
    10921 tatccagaag agtttaatag aacactacat aataaattcc gatctttaga tgatattgca
    10981 gtaacgggct atctctatca tcattatgcc ctactctctg gacgagcact acaaagttct
    11041 gacaagacgg aacttgtaca gcaaaatcat gatttcaaaa agaaactaaa taatgtagtg
    11101 accttaacta aagaaaggaa ttttgacaaa cttcctttga gcgtatgtat caacgatggt
    11161 gctgatagtc acttgaatga agaatggaat gttcaagtta ttaagttctt agaaactctt
    11221 ttcccattac catcatcatt tgagaaataa gttaaattat gaagaacctt tgagtgcaat
    11281 tcgaaggttc ttcattcata ttattcatat tttggagaaa ttatgttatc taatttaaaa
    11341 acaggaaata atatcttagg attacctgaa tttgagttga atggctgccg attcttatat
    11401 aaaaaaggta tagaaaaaac aattattact ttttcagcat ttcctcctaa agatattgct
    11461 caaaaatata attatataaa agatttttta agttctaatt atactttttt agcattctta
    11521 gataccaaat atccagaaga tgatgctaga ggcacttatt acattactaa tgagttagat
    11581 aatggatatt tacaaaccat acattgtatt attcaattat tatcgaatac aaatcaagaa
    11641 gatacctacc ttttgggttc aagtaaaggt ggcgttggcg cacttctact cggtcttaca
    11701 tataattatc ctaatataat tattaatgct cctcaagcca aattagcaga ttatatcaaa
    11761 acacgctcga aaaccattct ttcatatatg cttggaacct ctaaaagatt tcaagatatt
    11821 aattacgatt atatcaatga cttcttacta tctaaaatta agacttgcga ctcctcactt
    11881 aaatggaata ttcatataac ttgcggaaaa gatgattcat atcatttaaa tgaattagaa
    11941 attctaaaaa atgaatttaa tataaaagct attacgatta aaaccaaact aatttctggc
    12001 gggcatgata atgaagcaat tgcccactat agagaatact ttaaaaccat aatccaaaat
    12061 atataaaatg cgtaagatta cttttattat ccctataaaa cagtctttaa taaaacctga
    12121 ttgctttata cgcctctttt ttaatttatt tttgctaaaa aaattctcaa gtaaatacgg
    12181 attttctata ttagttgcag acaacagtaa cttcctttgg aaaaatatta ttaaattaat
    12241 tacaaaattt tacaaatgta attatattag tattaaatct cataatactt tttatacgcc
    12301 tgctaaaatt aaaaatgcag ctgccatcta tagttttaat accttgaatt caaattacat
    12361 tttattctta gatgttgacg ttttattatc ggaaaatttt attcaacatt taataaaaaa
    12421 aacaaaaacc aatatcgcct ttgattggta ccctgtttca ttcttaaaca aacaatttgg
    12481 gattataaat tttatattat tctcatataa aggtaatcta aatatagaag aatcattcat
    12541 tatacaaaca gggtttgtaa ctggcttaca attatttaat tctgattttt tctacaaaac
    12601 agctggatac aatgaaagct ttcttggcta tggctgtgaa gatattgaaa tgattcacag
    12661 agcaacatta ttattaaata ttagacctgc ctttaatgaa aatcatcaat attttacaga
    12721 tgatagagga tatatgcctt ctaaattaac cggatttcga aattattttt attatttgaa
    12781 aagagatgaa ttttcaaact tacagataac tcctaaacat ttctggcata agcgaaaaaa
    12841 taaatcaaaa tatctaaaaa atagatatca aaatgatgta aaaatgattc agattatgaa
    12901 agattttgat cgaaaatttc taaaaaatta acgagctgtc ttgcccatat gaatcctgat
    12961 tactttaatt taattatgaa aaatattctc gttaccggcg gcaccggttt tatcggctcg
    13021 cacaccgttg tttctttgct gaaaagcggc catcaagtcg tgattttgga taacctatgc
    13081 aattccagca tcaatatcct gccacgcttg aaaacgatta ccggccaaga gattccgttt
    13141 tatcaaggcg atatccgcga tcgtgagatt ttgcgccgta tttttgcgga aaaccgcatt
    13201 gattcggtga ttcattttgc cggcttgaaa gcggtgggtg aaagtgtggc cgagccgatg
    13261 aaatattatg ataataatgt ttccggcagc ttggtgttgg cggaagaaat ggcgcgtgcg
    13321 ggcgtgttta gcattgtgtt cagttcttcg gcgacggttt atggcgatcc gggcaaagtg
    13381 ccttataccg aggatatgcc actgggcgat accaccagcc cttacggcac atcgaaatca
    13441 atggttgagc gcattctcac tgatattcaa aaagccgatc cgcgctggag cgtgattttg
    13501 ttgcgttatt tcaatccgat tggcgcgcac gaaagtggtt tgattggcga gcagccaaac
    13561 ggcatcccga ataatttgtt gccttatatc tgccaagtgg cggcaggcaa actgccgcaa
    13621 ttggcggtat ttggcgatga ctaccctacc cccgacggca cggggatgcg tgactatatt
    13681 catgtgatgg atttggcaga aggccatgtc gcggctatgc aggcaaaaag taatgtagca
    13741 ggcacgcatt tgctgaactt aggctccggc cgcgcttctt cggtgttgga aatcatccgc
    13801 gcatttgaag cagcttcggg tttgacgatt ccgtatgaag tcaaaccgcg ccgtgccggt
    13861 gatttggcgt gcttctatgc cgacccttcc tatacaaagg cgcaaatcgg ctggcaaacc
    13921 cagcgtgatt taacccaaat gatggaagac tcatggcgct gggtgagtaa taatccgaat
    13981 ggctacgacg attaagttga cctgatacag gccgtctgaa agagatgttt tcagacggcc
    14041 tctttatctg aaaaacacac attctgtctg ctataatctg tttatatttt ttggctatcc
    14101 tctgaaattt atgagaaaaa tccttgttac cggcggtgcg ggctttatcg gttctgccgt
    14161 tgtccgtcat attatcc
//
LOCUS       DAWFLT010000053        13899 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWFLT010000053 DAWFLT010000000
VERSION     DAWFLT010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13899
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.053"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(570..905)
                     /locus_tag="WGR74_001454"
     CDS             complement(570..905)
                     /locus_tag="WGR74_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ0905852.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFAQGL
                     PPGVGDFFR"
     gene            complement(986..3115)
                     /gene="dnaX"
                     /locus_tag="WGR74_001455"
     CDS             complement(986..3115)
                     /gene="dnaX"
                     /locus_tag="WGR74_001455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ0905853.1"
                     /translation="MAYQVLARKWRPKTFADLVGQEHVVKALRNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYEPAALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAREMAACAV
                     GFDNALGELAILLQQLALIQAVPNALAHDDPDSDILHRLAQTIGGEQIQLYYQIAVHG
                     KRDLGLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSVQTAEKEAAAK
                     KPQPRPEAETAQTSVQTASAAAMPSEGKTAEPVSNQENNDVPPWEDAPDEAQAAAGTA
                     QTSAKSIQTASEAETPPENQVSKNKAADNETNAPLSEVPSENPIQTTPNNEALETEAF
                     AHEAPAKPFNGYSFPDDDCPVEDNAEIPPPDWEHAAPADTAGGGADEEAEADGIGGNN
                     TPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARAT
                     ADKKRLDKIRDTLAQAYGLQLTLQTQDWQDESGRETPAMQDKRVQAEDRQKAQALLEA
                     DPAAQKILQAFGAQWQPESLELAANRP"
     misc_feature    complement(1887..1926)
                     /gene="dnaX"
                     /locus_tag="WGR74_001455"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            complement(3210..5186)
                     /gene="mutL"
                     /locus_tag="WGR74_001456"
     CDS             complement(3210..5186)
                     /gene="mutL"
                     /locus_tag="WGR74_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ0905854.1"
                     /translation="MSRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAIE
                     VELAGGGIRLIRVSDNGSGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQKDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRADLTE
                     SVGNAGEVLHEITGVTPAPMPSENDSENLFNRASDYPTGNKPDTRNAFGASGKTAPMP
                     YQAARAPQQRSLSLRESRAAMNTYAELYKKTDDIDLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGRLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAAPAML
                     GKSDVVSLARDVLGELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNVLLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            5330..5515
                     /locus_tag="WGR74_001457"
     CDS             5330..5515
                     /locus_tag="WGR74_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ0905855.1"
                     /translation="MYHYQSDATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            5526..6194
                     /locus_tag="WGR74_001458"
     CDS             5526..6194
                     /locus_tag="WGR74_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ0905856.1"
                     /translation="MTTHLSNVAPDLQNYLNSIGEPEHPVLTRLREKTEHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIVQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDPRIVPITLPVGDGLTL
                     LLKK"
     gene            6194..6910
                     /locus_tag="WGR74_001459"
     CDS             6194..6910
                     /locus_tag="WGR74_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ0905857.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRLEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGTLVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWLSL
                     IQTYPGSPAAKRAAAAIRKR"
     gene            6958..7443
                     /gene="purE"
                     /locus_tag="WGR74_001460"
     CDS             6958..7443
                     /gene="purE"
                     /locus_tag="WGR74_001460"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ0905858.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANENPELAQKLADFRAKQEQTVLNMELEQ
                     I"
     gene            complement(7626..9080)
                     /locus_tag="WGR74_001461"
     CDS             complement(7626..9080)
                     /locus_tag="WGR74_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ0905859.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPTEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(9077..9778)
                     /locus_tag="WGR74_001462"
     CDS             complement(9077..9778)
                     /locus_tag="WGR74_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ0905860.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEVERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            complement(9775..10554)
                     /locus_tag="WGR74_001463"
     CDS             complement(9775..10554)
                     /locus_tag="WGR74_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ0905861.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            10839..12245
                     /locus_tag="WGR74_001464"
     CDS             10839..12245
                     /locus_tag="WGR74_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0905862.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADL
                     NESPLDMQLAVISYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQIAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWVFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVAGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGTNLTRNTPS"
     gene            12357..13883
                     /locus_tag="WGR74_001465"
     CDS             12357..13883
                     /locus_tag="WGR74_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ0905863.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
BASE COUNT         2828 a         3760 c         3887 g         3424 t
ORIGIN      
        1 ttttgcccga cggggtgaaa aatacagttg ctacagcccg acctacgccc gctttgcctc
       61 tatcctgcgc ccttttatgt gcaacacttt gcacttgctg aacaaaattc aaacgaccct
      121 ttatatcaaa tgcaaaaaat atgccgtcat tcccgcgaag gcgggaatcc agacccctcg
      181 gcacggaaat ttatcgagta aaacggtttc tcagattcta cgttctagat tcccgcgttc
      241 gcgggaatga cggggtgtaa gtttctgctc cgacggggtt ggattcccgc tttcacggga
      301 atgacgaagt ttcagacggc atttaagcgg tacggatgtg taaataatgg tagggtgggc
      361 ttcagcctgc cgattcccgc tattcttgcc gtttttgcgt tcttatcatt ctcactgttt
      421 ttaccgttca cgccgaatcc tgcaaactga tgccgtcatt cccgcgaagg cgggaatcca
      481 gacccgtcgg cacggaaatt tatcgagtaa aacggtttct tagattctac gttctagatt
      541 cccgcctgcg tgggaatgac ggtcggggat cagcggaaga agtcgcccac tccggggggg
      601 agtccttggg cgaatgcgcc cattgttttg tttgcggttt cttcggcttt gccacgggcg
      661 gatttgaggg cggcgaggat gaggtcttca agcatttctt tgtcgtcggc ggcttcttga
      721 atcaaatcgg ggctgatgtc gattttgcgt acttcgtgcg cgcaggtcat tgtgattttg
      781 accaagccgt tgcctgcttc gccttcgatt tcggtttcgg caagtttggc ttgcgctttt
      841 ttcatatttt cctgcatttg ctgcgcctgt ttcatcaggc cgcctaatcc ggcttttccg
      901 aacatactga atactcctgt ttgtgtttaa atgaatgagg gaaacggcaa tgccgtccga
      961 agggttcggg cggcattata tctgtttatg gccggtttgc cgccaattcc agtgattcgg
     1021 gctgccattg cgcgccgaat gcttggagga ttttttgtgc ggcggggtcg gcttcgagca
     1081 atgcttgtgc tttttgcctg tcctcggctt ggacgcgctt gtcctgcatc gcgggggttt
     1141 ctctgccgga ttcgtcctgc cagtcttggg tttgcagggt gagttgcagc ccgtaggctt
     1201 gggcaagggt gtcgcggatt ttgtcgagac gttttttgtc ggcggtggcg cgtgcttcgg
     1261 cggtcattgc caaaaccatc aggccggtgt cgggatggta ttccgtccac gcggagtgtt
     1321 gcgccggcat ttgcgccgcg ccgagtttgc gggcgaaatg ccggacgatg gctgcccagt
     1381 tttcggtgga aaactcgggc ggtggggcgg acggtgtgtt gtttccgccg atgccgtctg
     1441 cttctgcttc ttcgtccgcg cctccgcctg cggtatcggc aggggcggcg tgttcccaat
     1501 cgggcggcgg gatttccgcg ttgtcttcta cggggcagtc atcatccgga aagctgtaac
     1561 cgttgaaagg ttttgcagga gcttcgtgcg caaatgcttc cgtttcaagg gcttcattat
     1621 tcggtgttgt ctgaatgggg ttttcagacg gcacttcgga caagggggca ttggtttcgt
     1681 tgtcggctgc cttgttcttg gaaacttggt tttcgggcgg cgtttcggct tcggatgccg
     1741 tctgaatgct ttttgccgac gtttgcgccg tgccggctgc ggcttgggct tcgtctggcg
     1801 cgtcttccca aggcggaaca tcgttgtttt cttgattgga aacgggttcg gcggttttgc
     1861 cttcagacgg cattgccgct gcggatgccg tctgaacgga tgtttgggcg gtttccgctt
     1921 cagggcgcgg ttggggcttt tttgcggcgg cttccttttc ggcggtttgt accgatgggg
     1981 attgaagttc ggtattttca atcacggcgt ttgcatcaca cgaagctgcc gccaagggcg
     2041 caaacgccag catacgcagc agggtcatca taaagccggc gtattcgtcg ggggcgaggc
     2101 ccaagtcgcg tttgccgtgg acggcgattt ggtagtaaag ctggatttgt tcgccaccta
     2161 tggtttgggc gaggcggtgc aaaatatcgg aatcggggtc gtcgtgcgcc aaggcattcg
     2221 gcactgcctg tatcagggcg agttgttgca gcagtatggc gagttcgccc aaggcgttgt
     2281 caaagccgac ggcacacgcc gccatttccc gcgctttggc ggtcagggct gcgccgtctt
     2341 ggttgatgat gcctgtcagc agttcgtaaa ggtattgttt gtcaaccgcg ccgatcattt
     2401 ggcggacatc gttttcggca actttgcccg aacccagggc gatggcttgg tcgagcaggc
     2461 ttaaggcatc gcgcatcgat ccggcggcgg cgcgtcccaa aagttgcagg gcggcgggtt
     2521 cgtaggcgat tttttcgctg tcgaggacgt gggcgaggtg gtcggcaacc tgctgcgcgg
     2581 tcatattgcg caagacgaat tgcaggcagc ggcttaatac ggtaacgggg actttgtgcg
     2641 gatcggtcgt ggcaaggatg aatttgacgt gttcgggcgg ctcttccagc gttttgagca
     2701 tagcgttgaa cgcgcttttg gaaagcatat gcacttcgtc gatgatatag actttgtatt
     2761 tgccggcagt cggcgcgtat tgggcgtttt ccaagacttc gcggatgttg tcgatgcctg
     2821 tgttggaggc ggcgtcgatt tcgagcaggt cgacatagcg tccggcatcg atttgggtgc
     2881 agctttggca cacgccgcaa ggttcgccgt gttgcgcgtt ttcgcagttg aggcttttgg
     2941 caaggatgcg ggcgatggtg gttttaccta cgccgcgcgt accggtcagc aggtaggcgt
     3001 ggtgcagcct gccttcgtcc agcgcgttgc gcaaggcttt gacgacgtgt tcctgaccga
     3061 ctaagtcggc aaaggttttc ggacgccatt ttcgggcgag aacttgatag gccatgtttt
     3121 tctcttggtt tcggtcgtga tgtttctgtc ggtgcttcgg aatgccgtct gaacggcggt
     3181 ctcgggcggc gtattctagc actttcggct tattgtccgc gcaagaacag tgcgtccaat
     3241 tctttcaaag tcagtttgac ccacgtcggc ctgccgtggt tgcactggtt gctgcgcggc
     3301 gtgttttcca tatcgcgcag aaggacgttc atttcgggca gggtaagccg gcggccggcg
     3361 cggatcgagc cgtggcagga catggtggcg aggatgcggt tttcgtgttc ctcgatggtt
     3421 tggctgctgc cgacttgggc gagttcgccc aatacgtcgc gggcgaggga gacgacatcg
     3481 gatttgccca acattgcggg tgcggcgcgg acggcgaggg tgttgccgcc catatcggat
     3541 aattccagcc cgaagcctgc cagcgtttcg gcataatcgg caagggcggc gcattcttcg
     3601 tgggacgcgg caaaggttac gggaataagc aggcgttggc tttgcagcct gccgttttcc
     3661 tgacgctggc gtttcatttt ctcgtagttg acgcgttcgg cagcggcgtg catatcgatg
     3721 agcagcaggc tgtcttcggc ttgggcaaga atgtagatgc caagtaattg ggcaatggcg
     3781 aaaccgagcg gcggcagttc ggattgggag gggatgccgt ctgaaagcgg cgtatctgtt
     3841 tggggagcag gcgtttcaga cggcatattg ccgaaacgtg cctgctcgaa tcggcttaac
     3901 tcaaggtcga tgtcgtcggt ttttttgtaa agctcggcat aggtgttcat tgccgcgcgg
     3961 ctttcgcgca gggacaggct gcgttgttgc ggcgcacgcg cggcttggta gggcataggc
     4021 gcggttttgc ctgatgcacc aaaggcattg cgcgtatcgg gtttgttgcc tgtcgggtag
     4081 tcggatgcgc gattaaacag attttcgctg tcgttttcag acggcattgg tgcaggcgta
     4141 actcccgtga tttcgtgcaa cacttcgccc gcgttgccga cgctttcggt caggtcggcg
     4201 cgcgtgtcgg caagggcttt gttgagcgtg tggaacacga gttggtgtac ctgctgactg
     4261 tcgcggaagc ggatttcggt tttggtcggg tggacgttga catctacggc ttcgggcggc
     4321 aggtcgagaa agaggacgaa agcaggtgtc agcgcgttgt gcaatacgtc gcggtatgcc
     4381 tgcttgacag catggagcat gactttgtcg cggacaaaac gatggttgac aaagcagtat
     4441 tgtttgtcgg ttttgccttt ggcgaaagtc ggcttggcaa tcgcgccata gagccgcaat
     4501 gcgccattgc cgctgtcgat ttccaaggat gccgtctgaa agtcatcgcc gacaatggcg
     4561 gcaatccgtt catgcaggct ttgtgcaggg agtttgaaca cttgtttgcc gtcgcgtttg
     4621 agtgagaagg cgatgtgcgg atgcgccaga gcaagtcgtt cgagcatggt ggcgcagtgg
     4681 gcgtattcgg tgttttccga tttgaggaac ttgcgccgcg cgggggtatt gaagaagagt
     4741 tcggcagctt cgatggtggt gccgacgggg tgggcggctg ccgtagggct gctgagtttg
     4801 ccgtcttcgg ctttgacttg ggtcgcgtgc gaactgtctt tttgacggct ggtcagggtc
     4861 aggcggctga cggaggcgat gcttgccaag ccttcgccgc gaaagcccat gctggcaacg
     4921 tgttccaaat cgtttaaggt tttgattttg ctggtggcgt ggcggtggag cgcgagttcg
     4981 atgtcgtccg gatggatacc gctgccgttg tcgctgacgc gaatcaggcg gatgccgccg
     5041 cccgccagtt cgacttcaat tgctgttgcg cctgcatcga tactgttttc aacgatttct
     5101 ttcagggcgt tggcagggcg ttcgaccact tcgccggcag cgatttggtt gacaagatgg
     5161 tcgggcaggg cggcgattcg ggacataagg cgggcttccg ttgcaaaaaa cgtcttattc
     5221 tataataaaa ccccttatct ttctgcccgt attcttgata aaagccgttt atcccgtttg
     5281 gaaaatacca gtataatcac gccatattcc gtaaaattgg agcacaaaga tgtatcacta
     5341 ccaatccgat gccacacaat tcctcaaccg cctgattgaa gaaaaacctg agttggaaca
     5401 gcagcgtttg gaaaacaggg ggcttttgtg ggatgtcgaa ctcaatcccg aagaacagga
     5461 aaacttcgag gcggcaaaag tagcgaaaaa accttatacc tattaccaag actgaacccg
     5521 ccgaaatgac gacccatctg tccaatgtcg caccggacct gcaaaactat ctgaacagca
     5581 tcggcgaacc cgaacatccc gttttgacgc ggctgcgtga gaagaccgag catcaccgta
     5641 tgggcaaaat ggcgattgcg cgcgaacagg cggcggtttt ggtttggctg gcaaagctga
     5701 ttcgcgcgga aaaatatctg gaagtcggcg tgtttaccgg atacagcagc accgcgcttg
     5761 cattggcgtt gccagaacac gggcggatta ccgcctgcga catcaatgta acctttaccg
     5821 atacggcgcg tcaggtttgg aacgaggcag gtgtggcaca taaaatcagc ctgcacctgc
     5881 aacccgcatt gctgacattg gatgatttga ttgtacaggg tgaagccgga agctacgatt
     5941 tggcactgat agacgcagac aaaccgccca cgccgcaata tttcgagcgt tgcctcaaac
     6001 tcgtccggca aggcggcatc attgccattg acaatatttt gttgaacgga agggtgatgc
     6061 gcgaagcggc ttccgacgcg ccgcccagtg tcggcatcct caaagatttc aatcaaaacc
     6121 tgccgaacga cccgcgcatc gtccccatca ccctgcccgt cggcgacggt ttgaccctgc
     6181 ttctgaaaaa ataatgaaga ccaaattacc gctttttatc atttggctgt ccgtatccgc
     6241 cgcctgttct tcccctgttt cccgcaatat tcaggatatg cggctcgaac cgcaggcaga
     6301 ggcaggtagt tcggacgcta ttccctatcc cgttcccact ctgcaagacc gtttggatta
     6361 tctggaaggc acactcgtcc gcctgtcgaa cgaagtggaa accttaaacg gcaaagtcaa
     6421 agcactggag catgcgaaaa cacacccttc cagcagggca tacgtccaaa aactcgacga
     6481 ccgcaagttg aaagagcatt acctcaatac cgaaggcggc agcgcatccg cacataccgt
     6541 cgaaaccgca caaaacctct acaatcaggc actcaaacac tataaaagcg gcaggttttc
     6601 tgccgctgcc tccctgttga aaggcgcgga cggaggcgac ggcggcagca tcgcgcaacg
     6661 cagtatgtac ctgttgctgc aaagcagggc gcgtatgggc aactgcgaat ccgtcatcga
     6721 aatcggaggg cgttacgcca accgtttcaa agacagccca accgcgcccg aagccatgtt
     6781 caaaatcggc gaatgccaat acaggcttca gcaaaaagac attgcaaggg cgacttggct
     6841 cagcctgata cagacctatc ccggcagccc ggcggcaaaa cgcgccgccg cagccatacg
     6901 caaacgatag tttttccatt tttccgcttt ttaccccatc cgcaccaagg aggatttatg
     6961 atacaaatcg gcatcatcat gggcagcaac agcgattggc ccgttatgcg gcaggcagcg
     7021 cagtttcttg aagagttcgg cgtagaatat gaggcgcgcg ttgtttccgc acaccgcacc
     7081 ccggatttga tgttccaata cgccgaaacc gcacgggcgc gcggcatcaa agccatcatc
     7141 gcgggcgcgg gcggtgcggc gcatttaccc ggtatggttg ccgccaagac caccgtcccc
     7201 gttttgggcg tacccgtccc cagcaaatac ctgcgcggcg aagattcgct tttatcgatt
     7261 gtacaaatgc ccaaaggcgt acccgtcgcc acattcgcca tcggcgaggc aggcgcggca
     7321 aatgccgcat tgttcgccgt ttccatgctg gccaacgaaa accccgaact ggcgcaaaaa
     7381 ctggcagact tccgagccaa acaagaacag actgttttga atatggaatt ggaacagatt
     7441 taaaacccgc cgtccgcgaa tatatagtgg attaaattta aaccagtacg gcgttacctc
     7501 gccttgccgt actatttgta ctgtctgcgg cttcgtctcc ttgtcctgat ttttgttaat
     7561 ccactataca aatgccgtct gaagccctgt ttcgggtttc agacggcatt tttgcgaaca
     7621 actttttatg cctgttcttt ttggcgcatt ttttctgcca tcagttcgtg cagggttttc
     7681 ttcgccggtt tcatcggcac gcggttttgc gtccaaccca actgtttgcg cggggtcagg
     7741 ttgcggaact tggtggctgc ccaaccgaag gcgcggtagg ttttgctgcc gctgaaaata
     7801 ccgttgaatg tgcgccacgc catttgttcg ccgaaggtat gcgatgcgcc ttgccctcgg
     7861 atggggtgcg gcacggtttc ggtcggcgaa cgttgcgctt caacgcgcaa acgctgcatt
     7921 tgttcggtaa tcgggatgcg taccggacag acttccacac acgcaccgca catggtacag
     7981 gcggtcggca gatcgcgggt ggcatcaaga cccaacaggt gaggggaaat aatctcgcca
     8041 atcgggccgg gataggttgt gccgtatgcc gcgccgccga tgcgggtata aaccgggcaa
     8101 tggttcatac acgcgccgca acggatacat tgcagggtgc ggcgcatttg gtcttcggca
     8161 taagcctggc tgcggccgtt gtcgagcaga accaagtgca tttcttgcgg accgtctaat
     8221 tcctcactgc ggcgcggacc ggtaatcatg ttgaaataag tggtaatgtt ctgaccgatg
     8281 gcagaacgcg gcagcagact gtacaagggt ggaatatccg acagcttggc aactactttt
     8341 tcaatgccgg taatggcgat atgcacgggc ggaacggtgg tactcaagcg accgttgcct
     8401 tcgttttcca ccagacacag cgtacctgtt tcagcaacgg cgaagttcac gccactcaaa
     8461 ccgacatcgg cagtgctgta aatatcgcgc agggctttgc gggcgaagcc ggtcagttgg
     8521 tccacatcgt ctgtaagcgg tgtgccgagg ttttggtgga acagttcgct cacctgttct
     8581 ttggttttat ggattgcagg catcacgata tgggtcggtt tttcgcctgc catttggacg
     8641 atgaactcgc ccaagtcgct ttctaccgct ttaatgcctt ttgcttcaag ataatggttc
     8701 agctcgattt cctcgctgac catcgatttg cctttgacca tcagcttgcc gtttttggct
     8761 gtgatgatgt cgtggataat ttggcaggct tcggtcgggg tttctgccca gtgcactttc
     8821 acgcccaact tagtcaggtt ttcttccagc tgctccagca gggctggcaa tttagacaat
     8881 gagcgctgac ggacgtgttc gcacaaatcg cgcaggcttt gcagctcttc ttcgtcggtc
     8941 aaaacggctt tgcgtttggt catcagcata tccatcgcgg tacgcaggct tttgcgcaaa
     9001 ggcttgtctt gaagggaaat tgcggcgttt tgcttgaaag tttccggctt catgtgaaat
     9061 ttgatggttt gcgtagtcat gcgttttcct ccaaatcggc aggggaaatg tggtcgggca
     9121 ggatggcgag gatgaccaaa tcgcgcgggc cgtgcgcgcc gtaagcaagc gtcagttgga
     9181 tgtctgcggt tttggacggg ccggaaatca ggaatacatt ggtcggcata ccgttttcca
     9241 ccagtttttc gccttcgacg gcattatgaa actcgttgta catcttggac gtatcgaaca
     9301 ggcagaaatg cacgggcgga acgaggctta aagtacgcgg ttcttcgggg ctggaaaaca
     9361 gcatcagcgt gccggtgcgg gcgatgccgc attgcgcgcc gctgaagccc gcatcgatgt
     9421 tcgtgaaaaa ctcggttttc caagtatcga tttcgcgctc gaaggcaatc ggttcgatat
     9481 tgctgtccgc caatgcggca cgggcaattt gtccgtgttc ggtcgccaag ggcagcagga
     9541 tgtttttcaa acccttgcct tctgccgctt cgcggaaaac ctgcatccaa ttgcttttcg
     9601 tcacccaata aatttcggtt ttgaccgcac gcatagcggc agcccaatgt ttcagacgct
     9661 caacttcgct gccccaagaa acacccattt cacggtaata atcaaaaacc gcaggttctt
     9721 ccatcggcaa tgcgtcggct tttttcagtt ttgccaaaat attttcacgc gcgctcatgc
     9781 tttgcctccg gtgcgttcca acaagaagga tgcgatatgt ttcggacgcg gcatatccgg
     9841 ctcgtccttg gcgattttgc cgccgatgtt catcatacag ccgcagtccg cgctgatgat
     9901 ttcggttgcg ccggtttctt tcagcgcggc gactttgtct gttaccattg cgccggaaat
     9961 atcggcttgt ttgacggaga atgtgccgcc gaagccgcaa cattcgcttt cgtggtcgtg
    10021 gacgatgcgt tcgacgtttt ccataccgtc aatcagttgc cagcctgaaa gatggacatt
    10081 catttcgcgg cgggcggcgc aggaagtgtg aacggcgact ttgagcggtt cgcccttgtc
    10141 ttcgggtttg aaaccgatgg caagcaggaa atgggtaaac tcgatgatgc ggccggcgca
    10201 atccacagcc ctttcctcgt actcgctgcc tttaaacagc gtcggccagt ggtgtttcat
    10261 catgccgccg cacgagccgg acggcacgac gatcggccag ttttcaggga aaaggtcgag
    10321 ttgtgctttg gcgacatcga aggcttcggt cggatggccg gatgaatagg caggctggcc
    10381 gcagcagctt tgcgccatcg ggaaatggac gcgtatgccc tgctgctcga ttagggtaat
    10441 ggcatccatg cctgcttcgg gcataaaaag atcaaggacg caagtgccga aaaaatagac
    10501 atcggtcgga ttgctgtcgt atcggatgat ttttggggga atggttgcgc tcatgttttt
    10561 tgataacggg aaacccgttt ttctctgtag aaaggtaagc gtttacttta agtaattgac
    10621 tgttgcgggt caagtctaat tttaaaaaat aatccggttt ttcttacaaa ctgcccccat
    10681 aacgcttact gtaccttaat ctgatggttt tcgataataa ttatcattac aatgcaatgc
    10741 cggttcgttt gcttgtgaac attcaagatg ccgactctga cggcattcag acagcatctg
    10801 aaaacaataa cggcacaagg atggggtaca atcgccctat ggaaaaaaac accctatccg
    10861 cccgcgcacc atccccttgg ctgccgcttc tgctggcaat tgccattttt atgcagatgt
    10921 tggatgcgac cattttaaat accgcactgc ctgaaattgc cgccgacctg aatgagtcgc
    10981 ctctggatat gcaactggca gttatttcct acacgctgac ggttgccctg ctgattcctt
    11041 tgagcggtta tttggcggac aggttcggaa cgaaaaaagt ctttttcggt tcgattgccg
    11101 tttttatgct cggatcggca ttgtgcgccg catcgggttc gctgtttgaa ttgacgcttt
    11161 cccgtgtcgt tcagggcatc ggcggttcga tgctggttcc gataccgcgt ctgaccatct
    11221 tgcgtgtgta cgacaagtcc aagctgctca atgccatcaa ttatgcggtt atgcccgcat
    11281 taatcgggcc ggttttaggg cctttggcgg gcggttattt ggtcgaatac gcttcgtggc
    11341 actggatttt cctgctcaac ctgcccatcg gtctgctggg tttcatattg ggacgcaaca
    11401 tcatgcccga tattaaaggc agtaatatct ctttagactt caaaggttat ctgatttttt
    11461 ctgccgccgc gtgcctcttg ttactttcgg cagaaagcct gtcgcacgcg ctgcctccgt
    11521 attttgcact gttgccgctg tgcggcggac tgctgtttgc acgccgttat ttccgacata
    11581 tgaaaaccgc gtccaaaccg atttattccg ccgacctgtt tctgatacgc actttccgtc
    11641 tgggactggc gggcaatctg ttcagccgtc tcggcatcag ctcgattcct tttctgatgc
    11701 ccctgatgtt tcaaatcgct ttcggcttcg gcgcaagcct gtcgggttgg ctggtcgcac
    11761 ccgtcgccct gtcttcgctg ctggtcaaac cgctgattgc accgctcatg aaacgtttcg
    11821 gctaccgcac ggtactgctt tggaacacca agctgcttgc cgccttcatc atgctgctcg
    11881 ccctgcctga cggaaactcg ccgctgtgga tttgggtttt cctctcgctg gcgatcggcg
    11941 cgtgcaactc cctacagttt tctgccatga acacactgac cctcgccgat ttgcgcccgc
    12001 aacaaacagg cagcggcaac agcctgatgg cggtcaacca acagcttgcc atcagcatgg
    12061 gcattgttgc cggcgcatta atccttaaaa actggacatt tctgataccg gcttcttcag
    12121 gtctgcattc cgccttccgt atgaccctgc tcagcatcgg cggcatcacc cttgcatcat
    12181 cgctggtttt caaacggctg cacgtttcag acggcaccaa cctgacacgg aacacaccgt
    12241 cctgaagcgg tccacacgca aaacttttac ccgtttcaac gtttggatta tgataccgca
    12301 cttccatgcg cgccaacccc aaaacacagg caatgccgtc tgaaaccata tccctgatga
    12361 aaacacgcag cctaatttcc cttttatgcc tccttctctg ttcatgttct tcatggttgc
    12421 ccccactgga agaacggacg gaaagccgtc atttcaatac ttccaaaccc gtccgcctgg
    12481 acaacatcct gcaaatccgg cacacccctc ataccaacgg gctatccgat atctatctgt
    12541 tgaacgaccc ccacgaagcc tttgccgccc gcgccgccct tatcgaatct gccgaacaca
    12601 gcctcgattt gcaatactac atctggcgca acgacatttc cggcaggctg ctgttcaacc
    12661 tcgtgtacct tgccgcagaa cgcggtgtgc gcgtacgcct gctgttggac gacaacaaca
    12721 cgcgcggatt ggacgacctc ctgctcgccc tcgacagcca tcccaatatc gaagtgcgcc
    12781 tgttcaaccc cttcgtctta cgaaaatggc gcgcactcgg ctacctgacc gacttccccc
    12841 gcctcaaccg ccgcatgcac aacaaatcct ttaccgccga caaccgcgcc accatactcg
    12901 gcggacgcaa tatcggcgac gaatacttca aagtcggtga ggacaccgtt ttcgccgacc
    12961 tagacatcct cgccaccggc agcgtcgtcg gcgaagtatc gcacgacttc gaccgctact
    13021 gggcaagcca ttccgcccac aacgccacgc gcatcatccg cagcggcaac atcggcaagg
    13081 gtcttcaagc actcggatac aacgacgaaa cgtccagaca cgcgctcctg cgctaccgcg
    13141 aaaccgtcga acagtcgccc ctctaccaaa aaatacaggc aggacgcatc gactggcaga
    13201 gcgtccaaac ccgcctcatc agcgacgacc ctgcaaaagg actcgaccgc gaccgccgca
    13261 aaccgccgat tgccgggcgg ctgcaagacg cgctcaaaca gcccgaaaaa agcgtctatc
    13321 tggtttcacc ctatttcgtc cccacaaaat ccggcacaga cgcactggca aaactggtgc
    13381 aggacggcat agacgttacc gtcctgacca actcgctaca ggcgaccgac gttgccgccg
    13441 tccattccgg ctatgtcaaa taccgaaaac cgctgctcaa agccggcatc aaactctacg
    13501 agctgcaacc caaccatgcc gtccctgcca caaaagacaa aggcctgacc ggcagctccg
    13561 taaccagcct gcatgccaaa accttcattg tggacggcaa acgcatcttc atcggctcat
    13621 tcaacctcga cccccgttcc gcacggctca atactgaaat gggcgttgtt atcgaaagcc
    13681 ccaaaatcgc agaacagatg gagcgcaccc ttgccgatac cacacccgaa tacgcctacc
    13741 gcgttaccct cgacaggcac aaccgcctgc aatggcacga tcccgccacc cgaaaaacct
    13801 acccgaacga acccgaagcc aaactttgga aacgcatcgc cgcaaaaatc ctatccctgc
    13861 tgcccataga aagtttatta tagaaatata gtggattaa
//
LOCUS       DAWFLT010000054        13677 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWFLT010000054 DAWFLT010000000
VERSION     DAWFLT010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13677
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.054"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(51..536)
                     /gene="fxsA"
                     /locus_tag="WGR74_001466"
     CDS             complement(51..536)
                     /gene="fxsA"
                     /locus_tag="WGR74_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ0905864.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
     gene            complement(866..1174)
                     /locus_tag="WGR74_001467"
     CDS             complement(866..1174)
                     /locus_tag="WGR74_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905865.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHDYGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKELLADLDKELC
                     "
     gene            complement(1158..1460)
                     /locus_tag="WGR74_001468"
     CDS             complement(1158..1460)
                     /locus_tag="WGR74_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ0905866.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(1534..3897)
                     /locus_tag="WGR74_001469"
     CDS             complement(1534..3897)
                     /locus_tag="WGR74_001469"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ0905867.1"
                     /translation="MQFSYSWLKTQADTELSADKLEHLLTMSGLEVEEAGTAAPAFAG
                     VVVAEVKSVEKHPDADRLNVTRVDAGTGELVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSNGMLCSTDELGLPDDGVNGLHILPQDAPVGVNIREYLDLDDTVFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQSVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISVLVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSAAQFVQNERIGGLWYGAAMPEQWGEKTRNADFYDIKADVENLLKN
                     KAVEFVKTEHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAVNKLVQEIGVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3933..4451)
                     /locus_tag="WGR74_001470"
     CDS             complement(3933..4451)
                     /locus_tag="WGR74_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905868.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIVP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(4458..5522)
                     /locus_tag="WGR74_001471"
     CDS             complement(4458..5522)
                     /locus_tag="WGR74_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0905869.1"
                     /translation="MIKSFWLENFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(5699..6691)
                     /gene="pheS"
                     /locus_tag="WGR74_001472"
     CDS             complement(5699..6691)
                     /gene="pheS"
                     /locus_tag="WGR74_001472"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0905870.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKREALNEVKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLRNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
     gene            complement(7037..7396)
                     /gene="rplT"
                     /locus_tag="WGR74_001473"
     CDS             complement(7037..7396)
                     /gene="rplT"
                     /locus_tag="WGR74_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ0905871.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(7409..7606)
                     /gene="rpmI"
                     /locus_tag="WGR74_001474"
     CDS             complement(7409..7606)
                     /gene="rpmI"
                     /locus_tag="WGR74_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ0905872.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(7753..8274)
                     /gene="infC"
                     /locus_tag="WGR74_001475"
     CDS             complement(7753..8274)
                     /gene="infC"
                     /locus_tag="WGR74_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ0905873.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(8292..10205)
                     /gene="thrS"
                     /locus_tag="WGR74_001476"
     CDS             complement(8292..10205)
                     /gene="thrS"
                     /locus_tag="WGR74_001476"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ0905874.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKAAVAGKVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLNAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQLVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(10258..10334)
                     /locus_tag="WGR74_001477"
     tRNA            complement(10258..10334)
                     /locus_tag="WGR74_001477"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10298..10300),aa:Val,seq:gac)
     gene            10513..11628
                     /gene="tgt"
                     /locus_tag="WGR74_001478"
     CDS             10513..11628
                     /gene="tgt"
                     /locus_tag="WGR74_001478"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ0905875.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLKGLEELDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGVDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQAQFHENRARGTD"
     gene            11638..12720
                     /locus_tag="WGR74_001479"
     CDS             11638..12720
                     /locus_tag="WGR74_001479"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ0905876.1"
                     /translation="MPSEAASDGILPRYPTRTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAKRMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            12911..13379
                     /locus_tag="WGR74_001480"
                     /pseudo
     CDS             12911..13379
                     /locus_tag="WGR74_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692723.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
     gene            complement(13376..13666)
                     /locus_tag="WGR74_001481"
     CDS             complement(13376..13666)
                     /locus_tag="WGR74_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905877.1"
                     /translation="MAKYFTETGISVREYFDFFGEFVVLPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGDGNIGSMGLKVPR"
BASE COUNT         3516 a         3654 c         2991 g         3516 t
ORIGIN      
        1 tcaaaccgga aaaatgccgt ctgaatcttc agacggcatt ccattcatat ttattcgtct
       61 tttttgtgtt cgatggcgtt tcgggaacga ttgccgccgt aaggctcttc aaccgtatat
      121 tccccctcga taatatcgtc atcgcgggaa aagccctctt ttctgcccga ttggttcatg
      181 ttgaaaaaat tttccgcacc tcctgcctgc aacactgcac ctcccttaaa cggcagcagc
      241 agcaataccg ccaacaccga ggatacgaat cccggactca tcagacacac cgccgccacc
      301 gtataacgga taggccacaa catctgataa acggataccc tcccgccgct tctcattgcc
      361 gcgcccgcca ataaaagacc ggacagcccc gtatgcctga gcatcagcac accggcggca
      421 aaacctgccg ccatcaaaaa caacgtccag ccgccgccca accaatcggc aacccacaca
      481 atcgacataa tctccaaaaa cagcagcacc aaaaaaccga taccgaaaaa tctcattgac
      541 cgtcatcctt atatttaagt aaacagcaaa ccgcccgaac aagactccaa gcgacctgcc
      601 tgtaaatgat tacaaaacca tgtgcttcaa gccgaaacaa tgtgaaatca cgcaatatag
      661 tggattaaca aaaaccagta cagcgttgcc tcgccttagc tcaaagagaa cgattctcta
      721 aggtgctgaa gcaccaagtg aatcggttcc gtactatttg tactgtctgc agcttcgtcg
      781 ccttgtcctg atttttgtta atccactata attatttttt agcgtgtaaa acaaaaccgg
      841 ctgcatacct gcaaccggcc tcaaatcagc acaattcctt atccaaatcc gccaacagct
      901 ctttcagacc gtctccgatt tcctgaagcg taaccggacg gttgccgtcc gaacccgact
      961 ccgcaccttc tgcatacggt gcaaacgtgc tctgcaattt caacagtttc accacatcca
     1021 aacgggtgta gcgttcgccg ccgtaaccga caaccacacc gtaatcatgc tgccattgcg
     1081 caaaaccata ggggctgatt tgcaacagtc cgcacaactc gtccagcgtg aaatagcgtt
     1141 ttgcgggaac cttcaaatca acgttgtttg tcatagtagt gttccaccat gcttttgagt
     1201 ttctggctgg catggaaagt taccacgcga cgggcggtaa tcggcacttc ctcgccggtt
     1261 ttcggattgc ggcccgggcg ttgcggcttg tcgcgcaact ggaaatttcc gaaaccggaa
     1321 attttgattt cttcgccgct tgccaaagtg ctgcggattt cttcaaaaaa gagttcgacg
     1381 atttctttgg catcgttttt ggtgacgttg ctgactttat ccaccaaaat atcggccagt
     1441 tctgctttag ttagtgtcat atgattacct tctgttgtaa tagttgtgga ttattaccaa
     1501 tttttattta aaattcaagc gaatatttat tttttaactg cgaagccgcg cccctgccgc
     1561 cgttgccgcg ccaatcagtt ttccgataag cggctcgacc gcctcatccg tcagcgtgtt
     1621 ttccatatcc tgcaaaatca ctttgaccgc cacgctcttc atcccttcgg gcagacccgt
     1681 gccgcgatac acgtcaaaca cgccgatttc ctgtaccaac ttgtttaccg cgcctttcaa
     1741 gacaagcagc aaatcatcat ggctcatagc ttccggcatc acaaacgcca aatcgcggcg
     1801 caccggctgg aatttcgata cggcccgata gcgcgttttc ccgcattcca acacggccgc
     1861 catatcgatt tcaaatacca gcggcgcttg cggcaggtcg tatttttgca gccatttcgg
     1921 atgcagttcg ccgacaaagc cgatgacttt gccgtctgaa acgatattgg cggcgcgtcc
     1981 ggggtgcagg gcgggatgtt cggttttgac aaactcgact gctttgtttt tcaacagatt
     2041 ttccacatcc gccttgatgt cgtaaaaatc cgcattacgc gttttctcgc cccattgctc
     2101 gggcatcgcc gcaccgtacc acaaaccgcc gatacgttcg ttttgaacaa attgagcagc
     2161 cgaacccttg ccgaacacgc gggcgatttc aaacacgcgc acgcggtttt gcttgcggtt
     2221 caagttgttt tgcagaattt ccaccaagcc gccgatgagc gtggaacgca tcacggcata
     2281 ctgcgccgcc agcgggtttt gcaggcggat ggggtcggcg ttggcggcaa aatcttgttc
     2341 ccactgctcg tcaacgaagg catagctgac cacttcgcgg tagccgcgcg ccgccatttc
     2401 gttgtaaacg gcaaaacgcg gacggcgtgt ttcgggcagt tccagcattt tcagacggcc
     2461 tgacgtgtaa tcgtcgggga tgttttcata gccgtaaacg cgtccgattt cttcaatcaa
     2521 atcagcctca atttcgatgt cgaaacggaa gctcggcgcg gtaacgcgga agccttccgc
     2581 cgttttctcg ggctgcaaac ccaagtgttg caaaatggtt tccacctgtt cggcagggat
     2641 gtccacaccc aacacggttt tcagacggcc caaacgcaat ccgacctgct tcgcttcagg
     2701 caattcgcct tgcgcttcca ccatttctcc tgccgcaccg ccgcagattt gcaacaccaa
     2761 ttcggtggcg cgttcaatgg catccgcctg caaacggtaa tccacgccgc gctcaaagcg
     2821 gaacgaagaa tccgaaccga aaccgtactg gcgcgatttg ccggcgatga tttcgggcgc
     2881 aaaccaagcc gcttccagca cgatattttg cgtgccgtct gaaaccgcgc tcgccgcgcc
     2941 gcccattaag cccgccaaac tcaacacgcc tttttcgtcc gccacgacca gcgtattttc
     3001 agacagggaa acggttttct cgttcaggca ttccagcgtt tccccttcgc gcgcgcggcg
     3061 gatgtgcagg ctgccggaaa gtttgtcggc atcgaaaacg tgcatcggct ggccgatttc
     3121 cagcattaca taattgccga tgtccaccag cacggaaata ctgcggatgc cgctgcgctc
     3181 caaacgttgt ttcatccaat cgggagtagc cgctttcgca ttgacatttt caataacgcg
     3241 gctgataaaa cggccgcaat cggcaggcgc gttaatctgc acggactgtt ttcgactgcc
     3301 cgtgatcggc gcggtatgga tttcgggctg cctgaacgcg caccccgtca atgcggacac
     3361 ttcgcgcgca atgcctttga tgctcaggca gtcggcacgg ttcggcgtaa ttttcagcgt
     3421 aaacaccgtg tcgtccaaat ccaaatattc gcgaatgttt acaccgacag gagcatcttg
     3481 cggcagaatg tgcaggccgt tcacaccgtc gtcgggcaga ccgagttcgt cggtggaaca
     3541 cagcatcccg ttcgacacca cgccgcgcat cttggtcggc ttgattttga aattgcccgg
     3601 caacaccgcg cccggcaacg aacacggcac tttgatgccc gctttcacat tcggcgcacc
     3661 gcacacaatc tgaaccaact cacccgtacc cgcatcgact cgggtaacgt tcaaacggtc
     3721 tgcatcagga tgtttttcaa cagattttac ttcggcaaca accacgcccg caaaggcagg
     3781 cgcggcagtc ccggcttctt ccacttccaa gccggacatc gttaacaggt gttccagctt
     3841 atcggcggaa agttcggtat cggcttgggt tttcagccat gagtaggaga attgcatgtt
     3901 atgttctcaa ttaactaatt aactttagat tttcaagagc tagctaaacc tttttgaatg
     3961 gctaagtccc tattaattac ttctacagaa atgttccata aagaaatcaa ccgcaaagta
     4021 tctttcttat taaattcttg ctttattaat tcattcaatt gcccaagata attaagcggc
     4081 acatctggga taaccagtaa taatttgatt tctatgctac cccattgcaa atttgaaaaa
     4141 gcactaggca caattgtttc tttgatatct tctctgccta atattcccaa aatttcaaca
     4201 tttaatgcat tacaccattt ttgataaatc tctgaaatat attcatcaaa acgctcagga
     4261 gatctttctt gatttggaat actttgtttt gcctcaacaa ataacaaatt atcttcttgc
     4321 agatagataa actctactgt cttaatacct tcttgccttc tcttccggat atatggttct
     4381 atataaaaca ggttattttt tactagatta aaagttatac ctgattcagt gagatttatt
     4441 atttcactca taaaaaccta accaatccat gctgtttccg gagataaaac acctatttct
     4501 tctctgaaca atcgaataga ctcaccaata atttcattat ccggcatgct attttgtaaa
     4561 ttttcctgca cccaagtagt ctgttccttt tctttgccag gcataaaaac aggaatactt
     4621 tcactatgct ttctagctaa taaacacagc ttcttgataa caaaatatga atgcgtactc
     4681 ataaaaatct gtattcccaa tttggattta gataaggaaa acagaatttc tagaaattga
     4741 ttaattgctt tcggatgtag attagcttct ggctcatcaa taaataagat tgatccagga
     4801 ctcaaaaacc gatttcctaa tagagtgtct aatatcccaa tcttttttac cccctctgct
     4861 gtagaattga tagagaaagt ttgtctatct ttttcatata cccatgcctt ctttttatca
     4921 ttgtagctaa cataaccgcc aaacaatgct tctagatcct ttctagcttc cgctgcctca
     4981 gggtaattcc tcccttttgt tggtttaata tccaatgctt ttaccaaatc aatgtaagta
     5041 gcatcaaaac caaatctctt ttcttcctct gattttttaa ttacatcaaa tagtgataga
     5101 acttctttgg gtggtaaaaa aatagagtta gcgtttattc tgggagaaag ttcgtatagc
     5161 tccggtttaa ttagcttttt ggtagttcta ccaaaagaaa acaaaactga agaactatct
     5221 tctaaagtca tacttaactt aagaggccgt tcgtttggat tttcttttcc tcttgtaact
     5281 aaatcaccaa tttcctctac ttggaacgtc caatatagct tatctcgcaa ttcctcagaa
     5341 aaatcacgaa tattctttcc tttatgagct tcctcatgac tttttaaaac actatataat
     5401 gcctttaaaa taaaagtttt ccctttgcta ttttctgcaa taattaaatt tattttccct
     5461 aaattatcag ccttcaataa gctaatcggc ccgaaatttt caagccaaaa tgatttaatc
     5521 atataatccc cctaatatat atcaacaata tttgtacaag acacattgtg aaaagacagc
     5581 aaccacagca ccatagtaaa cgcatagcag gcggataatg gagcgacata gcgtttcatg
     5641 atttagcttt ctgaggtagg cacaggctac ttttttcatt tttaggcagc ctacacaatc
     5701 attttacaaa ctgcttcaaa aaattcaaat cgttatcaaa aaacaagcgc aaatcgttca
     5761 cgttgtaacg cagcatcgcg aaacggtcga gaccgatacc gaaagcgaaa ccggtgtatt
     5821 tttcgggatc gatattcaca tttctcaaca cgttgggatg caccatgccg cagccgccga
     5881 cttccagcca tttgccgttt tcgcccataa tgtcgatttc ggcagacggt tcggtgaacg
     5941 ggaagaaaga cgggcggaaa cgtacttgca aatcatcgcg ttcaaagaag cgacggataa
     6001 aatccgtgaa cactgctttc aaatcggcaa aagttacgcc ctcttctacc cacaaacctt
     6061 ccgcctgatg gaacataggc gagtgcgtgg catcgctgtc cacacggtaa acgcggccgg
     6121 gggcgataat gcggatgggc ggctcttttt tatcgagcat atagcggatt tgaatcgggg
     6181 aagtgtgcgt acgcaaaaca tcgccgtttt caacgtaaaa cgtatcctgc atcgcacggg
     6241 caggatggtt tgcagggatg ttcagggctt ggaaattgtg aaaatcgtct tcgatttcag
     6301 gcccgtccgc cacttcgaaa cccattccgt gaaagagttc gaccacacgt tgcaaggtca
     6361 gggttacggg atgcaggctg ccgccttcct gagcgcgtcc gggcagggta atatcgaggg
     6421 cttcggcggc aagtcgggct tgcagcttga cttcgttgag ggcttcgcgt ttggcattaa
     6481 aagccgtctg aaaccggttt ttgcattcat tgatatgcgc acctatggtt ttgcgctctt
     6541 caggcgacat ttgccccaaa gttttcagaa gtccggtcaa ctcgccggtt ttaccaagat
     6601 aacgggcttt gatttgttct agagcgttga agtcttgcgc agcttctact gcggcaatgc
     6661 cttctgcaac gatgcggttt acattttcca taatatcaag tctgtcaatt aatgatatac
     6721 cacctgaaac aacgaggccg cctgtatagc ctgacatatc agactcttca gacgacctta
     6781 ttttcttcac ggtcaatcca ttggataaat tttctgtata gtggattaaa tttaaaccag
     6841 tacggcgttg cctcgccttg ccgtactatc tgtactgtct gcggcttcgt cgccttgtcc
     6901 tgatttaaat ttaatccact atattttaac gcaaatttat ttaaaaaaat agactttaaa
     6961 gaaagaaaat caatcacata gaatttctgc aaacaaagaa aaaaggaagc cgcagcttcc
     7021 ttttcaattt tttggattaa gcagccaaag cagctttggc tttttcaacc aattgtgcaa
     7081 aagcggcttt atcgaacacg gccaaatcag ccaatacttt gcggtcgatt tcaatagagg
     7141 cgcgtttcag accgttcata aatttgctgt aagacaaccc gttttcacgc gcacctgcat
     7201 tgatacggac aatccacaat tgacggaatt ggcgtttgcg ttggcggcgg tcacggtacg
     7261 cgtattgacc ggctttcatt accgcctgct tggcaacgcg gtaaacgttt ttacgacggc
     7321 cgcggtagcc tttggctaac gcgaagattt tttggtgacg ggcacgagcg gtaacaccgc
     7381 gttttacgcg tggcatattc taaactcctt aagcgtaggg taacatttta gcaacagaag
     7441 ccaaatcgcg atcatttacc atagaggtac cgcgcagttg gcgtttgttt ttggtggtct
     7501 ttttagtcaa gatgtggcgt ttgaacgcat gagcgcgttt cacaccgccg ttacccagta
     7561 ctttaaagcg ttttttcgcg ctagacttgg ttttcatttt aggcatggga aaactccatt
     7621 cgttatcgga taaggcatta gggggtttta aaacatcagt ttcaaacact tgaacccaca
     7681 ataccacggt tttttgccgc aattgaaaac ttactccgaa gcggcaatcg gagtaagcgg
     7741 agaattatag ctttattttt tcttcggcgc aatcatcatc accatttggc gaccttccat
     7801 tttgggaaag gactcgattt gcgccacttc agccaaatct tcttttacac gttccaaaag
     7861 ttgcgcgccg agttgctggt gagccatttc acggccgcgg aaacgcaatg tcactttgac
     7921 tttatcgccg tcggcaagga agcggttaat gttgcgcatc ttgatttgat aatcgccctc
     7981 atccgtaccc ggacggaatt tgatttcctt gatttgcacc tgcttttggt ttttcttggc
     8041 ttcgtcgcgt ttcttggcct gctggtattt gtatttaccg taatccatca gtttgcacac
     8101 aggcggttta gcagttgggg aaatctctac caaatcgaca tcctgccctt cggccatagc
     8161 caaagcttca cgaactgaaa cgacaccaag ctgttcgcct gactcactga ttaaacgcac
     8221 ttctttggcg gtaatttcgc cgttgattcg tgcttcgcgt tcttgagcga tgatgaatac
     8281 tcctataaaa attaatgatt gacgagggca tcagtgattt cttgctgcaa ttgcgcaatg
     8341 aaatcatcca aatccaaaga acccaaatct tctgctttgc ggcgtaccgc cactttgttt
     8401 tcctgcttct ccttatcgcc gacaacgatt tgataaggga aacggtattg gctgttgtcg
     8461 cggattttgt aaccgatttt ttcgttacgc aaatccaact cggcgcggaa tcctgccgcc
     8521 tgcaatttgg cagccacttc ccgacaataa tctgcctgat tttcggtaat attcataatt
     8581 accaattgaa ccggagccaa ccataacggg aatgagcctg catggttctc aatcagaatg
     8641 ccgataaacc gctccaaaga acctaaaatg gcgcgatgca acataacagg acgcgcacgg
     8701 tcgttgtttt cagttacata ttcggcattc aaacgctccg gcaatacgaa atccagttgt
     8761 aatgtaccgc attgccaaga acgacccaag gcatctttga catgatattc gattttaggc
     8821 ccgtaaaacg caccctcgcc cggcaattcg ccccattcca cgccgcaggc agtcaatgcc
     8881 tcgcgcaaac cctgctctgc cttatcccac acgtcatctg aacctgcacg tttttcaggg
     8941 cgaagagaaa gcttgacgga tacatcatgg aaaccgaact gtttgtagat gcgaaccaac
     9001 aattcattga acgcacgagc ctcgctgacg atttgatctt cggtacagaa aatatgcgca
     9061 tcatcctgca caaaaccgcg aacccgcatc agaccgtgca gcgcaccgct cggctcattg
     9121 cggtggcaag aaccgaattc cgccaaacgc atcggcaaat ctcgatacga acgcaaaccg
     9181 ttgttaaaaa tttgaacatg acccggacag ttcatcggtt taaccgcata ttcgcgtttt
     9241 tccgaactgg ttacgaacat attgtctttg tagttgtccc aatggccgga tttttcccaa
     9301 aaggttttat ccatgatttg aggcgttttg acctctttat aaccggcggc attcagctct
     9361 ttccgcatat gctgctcaat cacttgccac aaagcccagc ctttgggatg ccaaaacacc
     9421 atacccggtg cttcgtcttg caagtggaac aaatccagtt gtttgccaag cttgcggtgg
     9481 tcgcgctttt cggcttcttc gatacgttga atataggctt tcaactcgtc ttttgtcgcc
     9541 caagccgtac cgtagatgcg ttgcagcatt tcattgttgc tgtcgccgcg ccagtatgcg
     9601 cccgccaact tggtcagttt gaagtttttc aggaaacggg tattcggaac gtgcgggccg
     9661 cggcacatat cgacatattc ctggtgatga tacatcccca tcgcttccac ttcgggcata
     9721 tcgtcaatca ggcgcagttt gtattcttcg ccgcgctctt gaaaaatttt aatcgcctcc
     9781 gcacgcggag tcatgatttt gaccacatca tagtcttggg caatcaattc tttcatacgc
     9841 gcttcaatgg cggcaacatc ttccggtgta aacggttttt ccgtggcgat gtcgtaataa
     9901 aagccctctt caatgacggg gccgataacc atttttgcat taggatagag ttgcttgacg
     9961 gcatgcccga caagatgcgc gcaggaatgg cggatgattt cgatgccttc ctgatctttc
    10021 ggagtaatga tttgaacagc agaatcttca acaatcgggt cgcacgcatc gaccaatttg
    10081 ccgtttacct tgcctgccac cgccgccttc gccaaaccgg caccgataga cgcagcaatt
    10141 tgagccacgg taacggggga ttcgtattgg cggactgagc cgtccggcaa ggtaatattc
    10201 aacatcaaac gctccgaaag attaaaaaat aacggcataa atgccgttat ggggatttgg
    10261 taggcacgat tggattcgaa ccaacgaccc ccaccatgtc aaggtggtgc tctaaccaac
    10321 tgagctacgt gccttcaaag aattttcgca ttttatcgag tcgttttaat ttttgcaaga
    10381 ggtatatgca tttctttatg caacattgtg gcaagatttg gggatgtcgt ctgaaaaacc
    10441 gtgggaattc atttataatg ccgtctttta aaacatattt cgacccctgt cgcccaactg
    10501 acattcaatc ttatgcttaa atttacttta cacaaaaaag acggatacgc ccgacgcggc
    10561 acgctggagt tgaaccacgg caaaattgaa acgccggtat ttatgcctgt cggtacttac
    10621 ggttcggtta aggcgatgaa cccgcaaaac ctgcacgaca tcaaggcgca aattattttg
    10681 ggcaacactt atcatttgtg gctgcgtccg ggcttggagg tcgtcgaaca gtttggaggg
    10741 cttcacggct ttatcggctg ggacaaaccg attttgaccg actcgggcgg ttttcaggtt
    10801 ttttctttgt cggatatgcg caagctgacg gaagaaggct gtacgttcaa aagcccgatc
    10861 aacggcgaca agctgttttt atcgcctgaa atttccatga aaatccaaac ggttttgaac
    10921 tcggacatcg cgatgcagct ggacgaatgc acgccgggcg aagcaacgcg cgaacaggcg
    10981 cgaaaatcgc tgcaaatgag cctgcgctgg gcggaacgga gcaaaaaagc ctttgaagat
    11041 ttgaaaaacc cgaacgcact gttcggcatc gtgcaaggcg cgatgtatga agatttgcgc
    11101 gaagagtcgc tgaaaggctt ggaagagctc gatttccccg gcttggccgt cggcggcttg
    11161 tccgttggcg aacctaagcc cgaaatgtac cgcatgttgc gcgccgtcgg ccctatcctg
    11221 ccggaacaca aacctcatta tttaatgggc gtcggtacgc ctgaagactt agtgtacggc
    11281 gtggcacacg gcgtggatat gttcgactgc gtcatgccta ctcgcaatgc gcgtaacggc
    11341 tggctgttta cccgttttgg cgatttgaaa atcaaaaacg ccaaacacaa gctcgacaag
    11401 cgtccgatag acgaaagctg cacctgctac gcctgccaaa acttcagccg cgcctacctg
    11461 caccatctgc accgcgccgg cgaaatcttg ggcgcacagt tgaacaccat ccacaatctg
    11521 catttctacc aagtcattat ggcggaaatg cgcgaagccg tcgaacaagg caaatttgcc
    11581 gactggcaag cacaattcca cgaaaaccgc gcacgcggca cagactgact ccctgcaatg
    11641 ccgtctgaag ccgcttcaga cggcattttg ccgcgttacc ctacccgcac tatttttcag
    11701 aaaccgatca tgctcccatt ccttccagaa ccctcccttt cctatacgca acaaaaccgt
    11761 accgccgttt tgctgttgaa cctcggcacg cccgacgcgc cgaccgcgca ggcggtacga
    11821 ccttatctga agtcctttct gaccgaccgg cgcgtcgtcg aactgcccaa atggctgtgg
    11881 tatcccattc tgcatggtct ggtactgacg ctccgcccca aaaaaagcgc gcacgcctat
    11941 gaaaaaatct ggtttaagga aggttcgccc ttagaagttt acacagcacg ccaagctgcc
    12001 gcgcttgcca aacgtatgcc cgacctcatc gtccgccacg ccatgaccta cggcaatcct
    12061 tcggtggcag acgtattgtc ggaactgaag gcgcaaggcg cgggcagact gctggtcatc
    12121 ccgatgtacc cgcaatatgc cgcttccagc agcggagcgg cagtagataa ggtgtgcgaa
    12181 caattactgc tgcaacggaa ccaaatgagc gtccgcaccg tttcgcgctt ttatgacgat
    12241 accggctaca ttgatgcgat gaaaaaccac atcctccgat attgggcgga acacggacgt
    12301 gggaaaaaac tgatgttgag ttttcacggc gtaccgcaga agcactacga cctcggcgac
    12361 ccctatcccg acgagtgccg ccacaccgcc aaactgcttg ccgaagcact ggaactgacc
    12421 gaagaccaat atgtcgtatc gttccaaagc caattcggca gggcaaaatg ggttacaccg
    12481 agcacgcagg atttgttcgg caaactgccc aaacaggggg taaccgagct ggacgtattc
    12541 tgcccgggct ttttggcaga ctgtttggaa accatggaag aaatcgccct gatggggcgg
    12601 gaacagtttt atgaagcagg cggcaaaagc taccgctaca tcccctgcct caacgacaat
    12661 cccgactgga tagacgcact cgtcgcactt gccgaagaaa accttggcgg ctggcgttga
    12721 aactgtttga cccatgtcta aaatgccgtc tgaacgccgt gttcagacgg cattttcccg
    12781 ctactttagc gtatgatggc atctatgctg gacaacacat caagtgcaac cttactaccg
    12841 attcggcaag aaaatctttc acccgctacg caaaatcaac aacaaggttt caaacattaa
    12901 ggaaacacca atgaacacaa cccgactgcc gaccgccctc gtcttgggct gcctctgcgc
    12961 cgccgcttct gccgccgaca acagcatcat gacaaaaggg caaaaagtgt acgaatccaa
    13021 ctgcgtcgcc tgccacggca aaaagggcga aggccgcgga accatgtttc cgccgctcta
    13081 ccgctccgac ttcatcatga aaaaaccgca ggtgctgctg cacagcatgg tcaaaggcat
    13141 caacggtaca atcaaagtca acggcaaaac ctacaacgga ttcatgcccg ccactgccat
    13201 cagcgatgcg gacattgccg ccgtcgccac ttatatcatg aacgcctttg acaacggcgg
    13261 cggaagcgtt accgaaaaag acgtaaaaca ggcaaaaaac aaaaaaaaac taaacagaca
    13321 aaatgccgtc tgaaaccggc aatccggctt cagacggcat tcaaatcaaa cctttctaac
    13381 gcggtacttt cagccccata gacccgatat tgccgtcccc gtcaacctcc ttcgcggtca
    13441 gcataaagcc gccgatgtca atacggccgc actcgaccgg ctcctgactg tcggaacgct
    13501 tatcgaaaag ctcggcaagg ctcaaaccct cttcgcccgc ttccagcctc aaaccgtaag
    13561 caagtgccaa atcacccgaa cgtgctgccg gcaaaacgac aaactcaccg aagaaatcaa
    13621 aatactcacg gacgctaata cccgtctcgg taaagtattt cgccatttta tagtgaa
//
LOCUS       DAWFLT010000055        13105 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWFLT010000055 DAWFLT010000000
VERSION     DAWFLT010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13105
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.055"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..440)
                     /locus_tag="WGR74_001482"
     CDS             complement(<1..440)
                     /locus_tag="WGR74_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905878.1"
                     /translation="MKKIIDSKVRETNFNQAWRVIRAMQIDNISDEQVVIVRMSCYLK
                     KDEIFKNIERYGYQSSDIKKISPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKSGETLYSNFKSIKNQCKNYFF"
     gene            complement(608..808)
                     /locus_tag="WGR74_001483"
     CDS             complement(608..808)
                     /locus_tag="WGR74_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905879.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
     gene            complement(923..1342)
                     /locus_tag="WGR74_001484"
     CDS             complement(923..1342)
                     /locus_tag="WGR74_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905880.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGEWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMYSGLNLNQYGVASP"
     gene            complement(1400..1765)
                     /locus_tag="WGR74_001485"
     CDS             complement(1400..1765)
                     /locus_tag="WGR74_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905881.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(1747..2292)
                     /locus_tag="WGR74_001486"
     CDS             complement(1747..2292)
                     /locus_tag="WGR74_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905882.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETERAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQ
                     GIGRHRPWESKSTDRSWKNRF"
     gene            complement(2455..2726)
                     /locus_tag="WGR74_001487"
                     /pseudo
     CDS             complement(2455..2726)
                     /locus_tag="WGR74_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3288..3760)
                     /locus_tag="WGR74_001488"
                     /pseudo
     CDS             complement(3288..3760)
                     /locus_tag="WGR74_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581921.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3849..4073)
                     /locus_tag="WGR74_001489"
     CDS             complement(3849..4073)
                     /locus_tag="WGR74_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905883.1"
                     /translation="MMKNKYILTKAAIGITAISIFYLLNQESSGKTEEPSYFLMFMFL
                     NLLWFEENKTVMAAVTAIIAAHFIFVALSD"
     gene            complement(4250..4858)
                     /locus_tag="WGR74_001490"
     CDS             complement(4250..4858)
                     /locus_tag="WGR74_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0905884.1"
                     /translation="MFCTVSHLRRLKGHDVLLTAFARALAQCPQLRLNIGGSGQEEQR
                     LKQQAADLGITHAVTFLGALQPEAVLDLMRNSDAFVLASRTETFGVVYIEALSQGLPV
                     IATRCGGAESIVSDGNGYLVPVDDDDALADALIKMYEHHSDFEPARLRENCLNEFGEN
                     AVIGRLIGIFRQAIAEYGKKIPVKYSGLTKIRTRRRSRRQYK"
     gene            complement(4890..5273)
                     /locus_tag="WGR74_001491"
     CDS             complement(4890..5273)
                     /locus_tag="WGR74_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0905885.1"
                     /translation="MYFFPRFPFIDIDCIRWVCAGLKAFKHYIRENGLPDLIHAHRMN
                     YAGILAQKISQKYGIPYVVTEHSSTITRGLIRPHQWQPMEKAAAHASALLAVSRHFAH
                     VLQHKYGCEWQYLPQHTGRNIQPTV"
     gene            5642..6346
                     /locus_tag="WGR74_001492"
     CDS             5642..6346
                     /locus_tag="WGR74_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ0905886.1"
                     /translation="MLRSILAASLLAVSFPTAAEALNYNIVEFSESAGVEAVQDTMSA
                     RFQVTAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQADAALEYTDFHVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     PGVEEISISVNGTVQF"
     gene            complement(6410..6976)
                     /locus_tag="WGR74_001493"
     CDS             complement(6410..6976)
                     /locus_tag="WGR74_001493"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ0905887.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(7042..7458)
                     /locus_tag="WGR74_001494"
     CDS             complement(7042..7458)
                     /locus_tag="WGR74_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905888.1"
                     /translation="MLKHLAFLLPAMMFALPAASAVLTSYQEPGCTYEGDVGKDGKPA
                     GKGTWRCQDGRNYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            complement(7510..8409)
                     /locus_tag="WGR74_001495"
     CDS             complement(7510..8409)
                     /locus_tag="WGR74_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ0905889.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSIMLEELVSYLGGWQD"
     gene            complement(8440..9897)
                     /gene="der"
                     /locus_tag="WGR74_001496"
     CDS             complement(8440..9897)
                     /gene="der"
                     /locus_tag="WGR74_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ0905890.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEKEEEEAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQTAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENADDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(10051..10680)
                     /locus_tag="WGR74_001497"
     CDS             complement(10051..10680)
                     /locus_tag="WGR74_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ0905891.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFAVLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELAKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(10681..11976)
                     /gene="hisS"
                     /locus_tag="WGR74_001498"
     CDS             complement(10681..11976)
                     /gene="hisS"
                     /locus_tag="WGR74_001498"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ0905892.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVGRWTRTYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNFLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAADLTNTLQQWKNA"
     gene            complement(12070..12612)
                     /locus_tag="WGR74_001499"
     CDS             complement(12070..12612)
                     /locus_tag="WGR74_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ0905893.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVL
                     KKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFT
                     HHPKRQTEFAVGQIRQARAE"
BASE COUNT         3323 a         3102 c         3006 g         3674 t
ORIGIN      
        1 aaaaaaaaat aatttttgca ttgattttta atagatttaa aattcgaata tagtgtttct
       61 ccagatttaa aaagtttatc tttatctata tatttgatgc tttccctatc caaaataaat
      121 atttcaaaca ttaaaaaatc attacatgac caagccaaat cataaatttg attcaaatgg
      181 gtatcataaa gataaaaata ataaggtttg ggaacaggta aaatatttaa tggaaaagga
      241 ggactaattt tcttaatatc tgatgactga tatccatatc tttctatatt cttaaaaatt
      301 tcatctttct taagataaca agacattctg acaatcacaa cctgttcatc ggatatatta
      361 tctatttgca tggcgcgtat aacacgccat gcctgattaa aattagtctc ccttacctta
      421 gaatctatta tctttttcaa agatgaaaat gccccttatc ttgaactctc cagttagaac
      481 ctggaagttt cgtacctctt aaatgtgtat taatatttct tatagtttca ttaaaatgcc
      541 acgcgctgcc gatttcaacg gtaattttcg taccgctttc ttcaaattcc aggtattccc
      601 ccattagcta acgcaaagaa gcagacgcca tttcggcttc gttatgataa acccgccttc
      661 cgttgatata gacttccgcc cctgtccgct ccaaattcca aaaattccgt actgaaattt
      721 ccatatcccg atattgtgca gaccatgttt tttcgaaggt tttcataaaa tttcctatac
      781 ctgtccaatc ggcacatatc aattgcatta ttatagtgga ttaacaaaaa tcaggacaag
      841 gcaacgaagc cgcagacagt acaaatagta cagaaccgat tcacttggtg tttcagcacc
      901 ttagagaatc gttctctttg agctaaggtg aggcaacgcc gtactggttt aaatttaatc
      961 cgctatacat ctcaatacga taaatattcc ttaagtcaaa atgcaagcct gaccgtacct
     1021 taactgtcaa aattttatta ttttttattg attttaaaac aatttctgca aaattctctt
     1081 cgctttctct cttttttaga agcacataag aaaaaataaa acttccccga ttaaattcat
     1141 aaatatgttt caaccattcg cctcctcttt ctgtaagaca agattcagtt tcattcttcc
     1201 ttattgtata aatatttcct tcacaaaatc tgaaataaat ccataaatcc atcttatcca
     1261 taattaaaga aaaagtttca cctcgagatt ttgtcaacaa ttcgcaaggt tgcgatgttg
     1321 caatcaaata gccgaaagac attttttacc tcatacatgg tcgaaatcag tttctgttag
     1381 ttcagaatcc attttttcgt caacaactga atccgcattt ttgaattaac gttttcatca
     1441 gctgccgttt atctaaaccg gcaggttcag tttcaaaata agccttatat gaagactgta
     1501 agcatttcag aaaaagatca tcagaagaca tatctgccga atcaaataca actgttttga
     1561 ttttggtact tacccaaaac cctttttgct ctttttctac tatacggaaa ttcagaatat
     1621 ttccaaccga atcaaaagca cggtaaacat catccatcaa atcctgcggc tctattttct
     1681 tttccaattc cgacaatcct tgaaatatat ccaaagacac atcttcaaat agaaaaaaag
     1741 gaggagttag aagcggtttt tccatgatct gtccgtagat tttgattccc aagggcgatg
     1801 acgaccaatt ccctgtccag gcaaagtctt gcccgtatta tccgtaactc gacgatgata
     1861 atggggaaat tttccaatag gatgacctgt tctattaccg aaaggggcta tccgcatatt
     1921 attgccgatt ttaatctcac gtccatattt agcaaaggaa acaacctttc ctgcggcgcc
     1981 tacaccacca ggaattgcgc ctaatccgcc agcaatagca acatctctaa cagaagctgg
     2041 tctgcctgtc gttgcataac taaaaccatg ctgtgtccac ataccaatgg cagcaccacc
     2101 caagatagcc aatggaagaa acgccctctc agtctccttc atctcctttt gagaaagttc
     2161 cgccaactgc atcggcgcat ctgcccgcgt gtggaacact tggtcttcaa atgcctgatt
     2221 gtccaagccg tttgccattg cgggggcaat catagacagc atcattacgg ctgcggtgat
     2281 ttgttttttc ataataactc ctttggatta caaggttgga aaatcaaagc cctgcttaga
     2341 acgtatgttg cacacccaat ttcaataggt aaatcagatt gcaaatccag caatttgaat
     2401 attgtcattg ttccgtgcaa aagggatttt tattgatgag ttgtgtactg ggtttcagct
     2461 tggcttttta gataatcttt tcacaagaac atacatcaat actgtccaaa aaaaagattt
     2521 gataaatata ttgcaacata ttttattcca cctccatccg aaacagaaac caatatattt
     2581 ttaattaata catagctgat tattacattt attattccta taatatataa ggatcttgcc
     2641 aaaatttttt ttgattttat tttaggaata tctcttatcc atgctaaaat acagcccaat
     2701 gtcgaaaaga aaataacagc aaacactacc ttacctcctc tcacttccaa ccaaaccgat
     2761 agcaggtttg gttggaagtt ggtttattta ttatttaacg gcgaatgtca gtgttcttac
     2821 ccgtagaacc tgcataacca tttatgccac cagcaacagt ccccactgct acacctcgca
     2881 taccattaat cagaattctt cctatttttt cgaatccact atttcttcca aagatgcagc
     2941 aaacgcaggt tgagcctaga cccaatctac gtttgctgcc gcatctgatc cctattgttt
     3001 cttatcctta catcttcctg ccttgtcaat caaataaaga cagaagacta tacaaaaact
     3061 gaccgacata ctgaaaatac ctatcccctt ccatgcaatc tccgcaccat tgacccaata
     3121 gattagactg agaaacaaca aagccacagt ataaatcaac gtaaaaaata ttgcgtaaag
     3181 cacaataaag ggaactttta tctcacggtt gttttttata atatattcag caacttgatt
     3241 tccgaatata cctgataaaa aatatagtat tagatcatcc atttcgttta tcttctatgt
     3301 tttcccattg cggcgctaga agactgattt aatgctgcac catttgcacg aataactgca
     3361 ttaggaatag catttccccc tttccaagca gaacgggcaa aaacactagt agtaatccct
     3421 gcaccgcgca acatcgcact gctatatccg ccgcctatca taccaccagc ggttgcagcc
     3481 aaggtacttc ttgttgaagc aaattgccct gttttaattt tagaaatgcc gtgattagcc
     3541 caagcactaa ttgcccctcc catcaaagca cctgccacaa taggaagaaa cgccccctca
     3601 gtctccttca tctccttttg agaaagctcc gccaactgca tcggcgcatc tgcccgcgtg
     3661 tggaacactt ggtcttcaaa tgcctgattg tccaagccgt ttgccattgc ggggcgatta
     3721 tagacagcat catcgtggct tcagtgattt gttttttcat aacaaatttt tagggaggag
     3781 ataatgttcc atcaaaatcc tcctgaaagg ctgcctgaaa tttccaagca gcctttttga
     3841 ttgcttaatt aatcggatag tgcgacaaat ataaagtggg cggcaattat tgccgtaacg
     3901 gcagccataa ctgttttatt ttcttcaaac caaagcaagt tgagaaacat aaacatcaag
     3961 aaatatgatg gttcttcagt tttgccgctg ctttcttgat ttagaagata aaatatagat
     4021 atggcggtta tgccgattgc tgcttttgtt aaaatatatt tgtttttcat catcttgtat
     4081 ggtttttcat gcaagtttca acaataaaat tcggaataag atagagagat gttgatatat
     4141 cagataaata tatagtggat taaatttaaa ccagtacagc gttgcctcgc cttagctcaa
     4201 agagaacgat tctctaaggt gctgaagcac caagtgaatc ggttccgtac tatttgtact
     4261 gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc actatatttc accggtattt
     4321 tcttaccgta ttctgcgatt gcctgtcgga aaatgccgat caacctgcct ataacggcat
     4381 tttcgccaaa ttcgttcaga cagttttccc taagtcgggc aggttcaaaa tcagagtggt
     4441 gttcatacat tttgatgagt gcgtcggcaa gggcatcgtc gtcgtcaaca ggaaccaaat
     4501 atccgttgcc gtctgaaaca atagattccg caccgccgca gcgtgtcgca atgacgggca
     4561 atccttggga cagtgcttcg atatagacca cgccgaaggt ttcggtgcgg ctggcaagga
     4621 cgaatgcgtc gctgttcctc atcaaatcca agactgcttc gggctgcaat gcgcccaaaa
     4681 atgtaacggc atgggtaatg cccaagtctg ccgcctgctg tttcagccgc tgttcttcct
     4741 gtccgctgcc gccgatgttc aggcgcagtt gcgggcattg tgccaacgcc cgggcaaagg
     4801 cagtgagtag gacatcgtgt cctttgagac ggcgaaggtg cgagacggtg cagaacacga
     4861 aatgcggttt gttgtttttt tctctttgtt caaacggtcg gttgaatatt ccgcccagta
     4921 tgttggggga ggtactgcca ttcgcagccg tatttgtgtt gcaggacgtg tgcgaaatgg
     4981 cggctgacag cgagcagtgc ggaggcgtgc gccgctgctt tttccatcgg ctgccattgg
     5041 tggggacgga tcagaccgcg cgtgatggtg ctgctgtgtt ccgtgacgac ataggggatg
     5101 ccgtattttt gggaaatttt ttgggcgagt atgccggcat agttcatgcg gtgggcgtga
     5161 atcaggtcgg gcagcccgtt ttcgcggatg tagtgtttga aagctttcaa acccgcacac
     5221 acccagcgga tgcagtcgat gtcgatgaac ggaaagcggg ggaagaaata tatgccgtgc
     5281 catgcataga tgtccaaacc gctttgccga tattttgcaa aaccgtaagg ccggtcagga
     5341 tgcttgctgt ttctttccgc aagtagcgga acatcggtgc aagcacggcg gttttgatgc
     5401 ctttcctctg caatgccagt gcctgatttt gaaaaaaatc ccgtccacat cctgttcgga
     5461 ttgcggatac catgagggga tgacgaggac gtgcaagggt tcgggcatag tgggattccg
     5521 tatcggaaag gcggttatta taagacagac gcagaccgaa tatttaaatt gttgccttac
     5581 gctaatgcaa tttagcgcgc cgatatgcta gattggcggt tttataagta aggatacgga
     5641 tatgttgcgt tctattttgg cggcttccct gctggcggta tcttttccga cggcggctga
     5701 agcattgaat tacaatattg tcgaattttc tgaatcggcg ggtgtcgagg cggttcagga
     5761 tacaatgtcc gcacgtttcc aagtgacggc ggaaggacgg gacaaaaatg ccgtcaatgc
     5821 cgagtttgtt aaaaaattca acaatttcac cagaaaatca aaaaatggta gctttaaaac
     5881 cgaattggta tcgcgcagtg cgatgccgcg ctatcaatat accaacggca gacgcattca
     5941 aacaggttgg gaggagcgtg cggaatttaa ggtcgagggt agggattttg atgcgttgaa
     6001 ccgttttatt gccgatgttc aggcagatgc cgcgttggaa tatacggatt tccatgtgtc
     6061 gcgcgaacgc cgcaacgagg tcatcgatca ggtcagcaag gatgccgttt tgcgtttcaa
     6121 ggcgcgtgcc gaaaagttgg cgggcgtttt gggtgcgtcc ggttataaaa tcgtcaaatt
     6181 gaatttggga cacatcggca gccatatcgc gggaggggga gctgctcagg caaaaatgct
     6241 tcgtgccatg ccgatggcgg caagcgtcaa tatggagggt gcggattccg ccgcgcctgg
     6301 tgtggaggaa atcagcatca gcgtcaatgg gacggttcag ttctgatttg aggtgaacgg
     6361 caaatgccgt ctgaaacccg acgataaggg ttcagacggc atttatattt caggctttgg
     6421 gcagggtaac gccggtttgc cccatatatt tgccgttgcg gtctttgtat gaggtttcgc
     6481 agatttcgtc gctctcgaag aagaggactt gcgccacgcc ttcgcctgcg tagattttgg
     6541 cgggcagggg ggtggtgttg gaaaattcga gggtaacgta gccttcccat tccggttcga
     6601 acggggtaac gttgacgata atgccgcagc gggcgtaggt ggatttgccc aagcagacgg
     6661 tcaggacgtt gcgcgggatg cggaaatatt cgaccgtgcg cgccagtgcg aaggaattgg
     6721 gcgggatgat gcagcagtcg tcttcaacgg taacgaagtt tttcgggtcg aagtttttgg
     6781 gatcgacgat ggtgctgttg atgttggtga agattttaaa ttcgtttgcg cagcggatgt
     6841 cgtagccgta gctggacgta ccgtaggaga tgatgcgttt gccgtcggct tctttgattt
     6901 ggttcggctc gaaagggtcg atcatgccga attcttcgct cattcggcgt atccatttgt
     6961 cggacttgat gctcataatg ttttccttgt ttcttgcagt gttcggacaa agcattgggg
     7021 gatgccgtct gaaaacgggg cttatttgtt tttgggcagc ttcacttctt taatcatgcc
     7081 gttttcgcat ttcataatga agagggtttc gccgttttgc gagacggtaa atctgccgtg
     7141 tgccaagcct tttttgaatg tgcccgagag taccatgttg cggaatttgg tactgtcgga
     7201 attgaacggt tcgataaata tttcgcggtt ggcggcaacg gtataaacgc cttgtccgtc
     7261 gaatttgcca tttttaaacg aaccggtata gttgcgcccg tcttggcagc gccatgtgcc
     7321 tttgccggcg ggtttaccgt ctttgccgac atcgccttcg taggtgcagc cgggttcttg
     7381 ataggaagtc agaacggcgg acgcggcggg gagggcgaac atcatggcgg gcagtaggaa
     7441 tgcgagatgt ttaagcataa gggttattcc attggatttt ggctgacggt ataaaagccg
     7501 tctgaaaaat caatcttgcc agccgcccaa ataggaaacc agctcttcca acatgatgct
     7561 gacggattcc gccatcagga tttgcgaggc gaaggcgaga ccggcggcat cgtcgccgtt
     7621 gctttcggct tcttcctgca atacgtcgag gtattggatg cgcttgagtg tgaagtcttg
     7681 cgtgaggata aaggcaattt gttcgcgcca caccaagccc agttgggtaa cggttttacc
     7741 gtttttgacg tgttgaacca cttcgtcggc ggttaaatct tgtttggata ctttgacgac
     7801 gggaacaata tcgcccgtgc ctttgagttc gcaatcgctg tccaattcaa aaccgccttc
     7861 gcaatgccct tgcaacagcc agccggtcat cagggaagag ggcgattgtt tggtattcgg
     7921 cagcgaggct tccaaaccgc ccaaagcttc gcgcagcttg gtcaggatgt tttccgcttt
     7981 ggcggaagcc gcgttattga cgagcaggta gccgtggcgg gtgttaaaca ccgcttctgt
     8041 acggctgctg cgggtaaacg ctcggggcag caggtcgtct gtaatttgct ctttaagctc
     8101 ttgtttttct ttgcgaccga cattgcgggc ttcattgttt tggatttccg ctaccttctc
     8161 ttccaaaata tcgcggatga cgccggcagg caggactttt tcctcttttt tcagggcgac
     8221 gcgcaaggta aagtcggcag ggaaaacgag ttccggggag aatgaaaccg gtgcggtaaa
     8281 gccttcgctg aaccagtcta agccttggca atgggtaaat tcagcttcag caagtttgtc
     8341 ggcaagtacg tccgcctcag gcagcttttc tttgttgagc ggataaaaac taatctgctt
     8401 gaaccacata atgtttcctg ttgtttgaaa tgtcgggaat tatttgctga attgtttttt
     8461 cacactgact ttggttttct tcttgaagcg gtttttctct tctttgcggc cttcgcgttt
     8521 ctcaatacgg ttgctcaggc tgacgcggcg cagcggtttt ttcttcggtt tgtcgtcggc
     8581 gttttcatac gggttttccg aaacattgta ttgaatccgc aacggcgtgc cttgcaggtt
     8641 gaaggctttg cggaacgttt gggtcagata gcgcgtatag ctgtcggaaa tcgcgtgcag
     8701 cgaattgccg tgtaccacaa ttacgggagg gttcatgccg ccttggtggg cataacgcat
     8761 tttcggacgc accaagccgg cacgcggcgg ttgctgacgc tcgattgcgc tttgcaggac
     8821 gcgcgtgatt ttcggcgtcg gcatcttaat catcgccgcg ttgtaggcag tctgaatgct
     8881 gtcaaacaaa ccgtctatac cgcgctcttt caatgcggaa ataaagtgga acttggcaaa
     8941 atcgaggaaa tacagtttgc ggttgatatc gcgtttcact tgctcgcgac gttcttcgct
     9001 gatgccgtcc catttattga ccgccaccac caaagcacgg cctgcctcca aagcgaaacc
     9061 tgcaatcgtc gcatcttggt cggcgatgtc ctgctgcgcg tccaatacca aaacagcgac
     9121 gtttgccgct tcaaccgcct gcatcgcttt gataacggag aatttttcca ctgcttcatc
     9181 cactttgccg cgacggcgca cacctgcggt atcgatgatg gtaaacggtt tgccttcgcg
     9241 ctcgaaatcg atatggatac tgtcgcgcgt cgtacctgcc atatcgaagg cgatgacgcg
     9301 ctcttcgccg agaatggcgt ttaccaaggt tgatttaccg acatttggac gaccgataac
     9361 ggcaaaaaca ggatgtttcg cctcttcctc ttctttttca ggctcaggga aggtttccaa
     9421 aatatcttca atcagataat acacgccgtc accgtgcgca cctgaaataa cataagggtc
     9481 gcccaaagca agttcgtaga actcggcggc aagcacggcc ctgttgccgc cttctccttt
     9541 attcacggcc aaataaacgg ggcgcgggct ttggcgcaaa cggtcggcaa tgattttatc
     9601 ttgcggcgtc agaccggtac ggccgtccac caaaaacaca accgcatcgg cttcatcgac
     9661 agcctgtaag gtttgttttg ccatttcgtg caaaatgccg ctgtccacaa ccggctcgaa
     9721 accgccggta tcgatgacca aataaggttt gctgccgact ttgccgtgtc cgtaatggcg
     9781 gtcgcgggtc aggccgggca ggtcgtgtac gagtgcgtct ttggtgcgcg tcaaacggtt
     9841 gaataaagta gatttgccga cgttggggcg gccgacaagc gcgatggttg gtttcatgat
     9901 tgagtctttc tgtgtcaagt gccgttcggg agaactgaac acgagcaggt gtccgttgga
     9961 cacggtaaat aggttcttca cggcaagagg ccataaagag ggagggtaaa atgccgtctg
    10021 ataaataatg gacggcattt cagacggcat ttatttcagc gaatcaagtt tcatttgaac
    10081 caattcgcga ccgacagaat cttgaggcat tttttctaaa gcctgtccgt agttttttaa
    10141 ggcttcctgg ctttttccct gtgcggcata gacatcgcct ttggtttcca tcagcagggg
    10201 ggcgaagtcc gcttcaaccg gcgtgtcgag tgcggcaagc gcggcatcgt attttttttg
    10261 ttgcaacaac acaacgccca gacgctgcgc cgccaacgcc tggatcaggc tgtctttttg
    10321 gttggacaat acccatttca aatggccttc ggcaacatcg taacgctgcg cgtcaaattc
    10381 ggttgctgcc gccatcagcg tggcttgggc ggcggaaatg gaatgggggt agctttgttg
    10441 gagcttggcc aattcggcat tgatttcgct ttgcggggct ttgttttgcg ccttttccac
    10501 gatgtttgcc agcaccgccg ccgcttcctg attttgggaa gccgcacggt tttggtaaac
    10561 cgtgtatccc aagtagccga gtgccgccaa aatcagcacg gcaaacagcc atttgcccgt
    10621 ggttttccaa aaatatttaa agttgtccaa ctcttgttgt tcttccaaat gggctgccat
    10681 ttatgcgttc ttccattgtt gtaaagtgtt tgtcaaatca gcggcggcga cggtttgctg
    10741 accgtgtgcg ccgttcatat ctttgagcgt aaccgtaccg ttcgccagtt cgtcttgcgc
    10801 gacaatcagg gcaaagcgtg cgccgctgtt gtcggctttt ttcatttgcg ctttcaggct
    10861 ctgatagccg gaatgctgca tcacattgaa accttgcgcg cgcaaggctt gtgcgtattt
    10921 catcacctgc aagtccgccc cttcgccttg gtgcattgca tagacatcag gcgcggcgtt
    10981 tacttccagc gagccgtatt cgctcaccaa aagcagcagc cgctcgatgc ccattgcaaa
    11041 gccgatagac ggcgcaggct taccaccaag ttcctcaatc aaaccgtcgt aacggccgcc
    11101 gccgcacaca gttgcctgcg cgccgagttt gtcggtcgtc cactcaaaaa ccgtttgatt
    11161 gtaataatcc aaaccgcgaa ccaagcgcgg attttcaata tattggatac ccaaaccgtc
    11221 caacatcgct ttgaagcgtg catagtggtt ttgcgaagcc tcgcccaaat aatccaccag
    11281 acgcggtgcc gcattgcaga tttcctgcaa atcagggttt ttcgtatcca aaacgcgcaa
    11341 aggattggtt ttcagacggc gtttgctgtc ttcatccaat ttatcttcat aacgggtcag
    11401 atattcaacc aatgccgcac ggtgtgccgc gcgttcctca cggttgccca agctgttgat
    11461 ttccaaagtc aggtattcgc ggatacccaa tttttcccac aagtcggcag acatcgcgat
    11521 aatttccgca tcgatatccg gcccttcaaa acccaaagcc tcgataccga cctgatggaa
    11581 ctgacgataa cggccttttt gcggacgctc gcggcggaac atcggcccca tataccacag
    11641 cttttgcggg ctgttgtaca gaaagttgtg ttcgaccacc gcacgcaggc aggaagccgt
    11701 accttcagga cgcaagctca aactcaaaga atcgtttgaa tcggagaagg tgtacatttc
    11761 cttgccgacc acatcggttt cctcgccgat ggagcggaca aacaaaccgg tttgctcgac
    11821 aatcggcgta cggatttgct gataaccgta agtgcgtgtc cagcggccga ccgtatcttc
    11881 aaacgcctgc caaaacgcag ccgtcagttt gaaatctttt tgctcgacag gcagaaggtc
    11941 gttcatgcct ttgacggatt ggattttttg tgccatttca agtaagaatg cttaaatcaa
    12001 attgcgggcg attatagcgg atttttaagg gtttgtgagg ttgggggtgg tttgcggacg
    12061 gcatttgact tactctgcac gtgcttgcct gatttgtccg actgcaaact ccgtctgccg
    12121 cttgggatga tgtgtgaaaa acaatttatt tgaaattgtc tcggcttttt tcggcacgac
    12181 cgccaaaatc ttacctgcca aatttccctc acgggtttgc caagcatcca aaaactgcgc
    12241 cctgctcatt gaaacatgac ccagcgacgg gtcggcaagc aaaaccgtat tgccgtctat
    12301 cccgcgcaat accgagaaat gatcatcctt gcggtatttc agatacacga tgacggggat
    12361 tttcaactgt gcgagctgct cgaaagacag ggcatagcct ttcgcttcaa aacccaaatc
    12421 tggcataatg cgccgcatat cctcaaacga cgcgcgcatc tgctccttat ccagcttttt
    12481 caacacttct tcttccgtca gcgtttgccc gtaaaaattg ttcaaaagcg tcgccaccga
    12541 agccgccccg caggaaaaat ccaaatcctg ctttacaata ttgaaatccc gcctttcttt
    12601 ccaactctgc actttgattt ttccgtaaac aacaggatta tcgttaaaca tcggtgcagc
    12661 attcaaacga taagataaag aaacgacaac acacgccaac agaaaaacat atttgaactt
    12721 catcatattg tccacataaa gggcagcctg aaaatctttc aggctgccct tgtcaaatta
    12781 ttcctagctt tcggcttttt tggcaaacca aacaatccga ttacccgcat aatactttcc
    12841 atttattgaa atccgacaag ccgcgcccaa aaaatgccat gcactgtcga tttccgcagt
    12901 aatctttgta tcgttttctt caaattccaa atattcaccc aataataaac ttgaaacaga
    12961 acgcgtgggc agcaagtgcc caccctacgc ctattcaaat aatttgatta aataaagaat
    13021 aaagaaaatg catcaggaac aaaattataa tctgccacct gactcacacc gcttttaaag
    13081 taaggggcat caaaatcaaa accgc
//
LOCUS       DAWFLT010000056        12920 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWFLT010000056 DAWFLT010000000
VERSION     DAWFLT010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12920
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.056"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            733..858
                     /locus_tag="WGR74_001500"
                     /pseudo
     CDS             733..858
                     /locus_tag="WGR74_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1009..2052)
                     /locus_tag="WGR74_001501"
     CDS             complement(1009..2052)
                     /locus_tag="WGR74_001501"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ0905894.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPDLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLGAIPLLP"
     gene            complement(2049..2342)
                     /locus_tag="WGR74_001502"
     CDS             complement(2049..2342)
                     /locus_tag="WGR74_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905895.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQAVGQRSDGIPANHTKEKP"
     gene            2596..3750
                     /locus_tag="WGR74_001503"
     CDS             2596..3750
                     /locus_tag="WGR74_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ0905896.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLESASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDTRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWQRPIPLGCETFHLA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQEKHEEVKKLIYQLIELDFLELL"
     gene            3759..3965
                     /locus_tag="WGR74_001504"
     CDS             3759..3965
                     /locus_tag="WGR74_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905897.1"
                     /translation="MKNWKQFTFFVILVIACYQLLYFLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQ"
     gene            4170..4529
                     /locus_tag="WGR74_001505"
     CDS             4170..4529
                     /locus_tag="WGR74_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ0905898.1"
                     /translation="MKRKNNIIELSIENLELIYGGGLGERILEGGANGAQIGGSIGAG
                     IGLGVGIVSGGIPAGPAIILGGTVGAAVGAVTGMGVAAYEYYQETQPKGTVTVGPISA
                     FAVDKSGNGYTDSTNYC"
     gene            4590..5306
                     /locus_tag="WGR74_001506"
     CDS             4590..5306
                     /locus_tag="WGR74_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HEZ0905899.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTAEGQILPASGVIRVYALDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHGNETRSLKATVERLENQELH
                     ISQQIDGQKRRIRLAEEMLQKYRFLSANDAVPKQEMMNVKAELLEQKAKLDAYRREEV
                     GLLQEIRTQNLTLASLPQAA"
     gene            5371..5826
                     /locus_tag="WGR74_001507"
     CDS             5371..5826
                     /locus_tag="WGR74_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ0905900.1"
                     /translation="MRSEQIIRAGRSGYIAIPNVEVGQQVDPSKLLLSIVPERTELYA
                     HLYIPSSAAGFIKPKDKVVLRYQAYPYQKFGLASGSVVSVAKTALGRQELSGLGMVSS
                     DLAKSNEPVYLVKIKPDKPTITAYGEEKPLQIGMTLEADILHEKRRLYE"
     gene            6549..6851
                     /locus_tag="WGR74_001508"
     CDS             6549..6851
                     /locus_tag="WGR74_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905901.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            7135..7374
                     /locus_tag="WGR74_001509"
     CDS             7135..7374
                     /locus_tag="WGR74_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0905902.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTNL
                     RALRSKYCPRPLQNSPKSPKCLGGNFGEFCKGLIL"
     gene            7435..9180
                     /gene="hrpB"
                     /locus_tag="WGR74_001510"
     CDS             7435..9180
                     /gene="hrpB"
                     /locus_tag="WGR74_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ0905903.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTVRKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISAFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEEGK
                     SDLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLVH
                     KTDLTDATGTETESGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQ
                     YQSSLAAERMLWRNRFHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAY
                     LNRWQLDGKLSYKRGTGMRQSMPAPEENGGDILPGTSRMKIITAGLDAAAPFILGKQQ
                     FFYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLSGERGFYWQNTLGWHFHPR
                     HQFYLGADYGRVSGESAQYASGKRLAGAVAGFRGSHKVGGIFAYDLFAGKPLHKPEGF
                     QTANTVYGFNLNYSF"
     gene            9289..12240
                     /locus_tag="WGR74_001511"
                     /pseudo
     CDS             9289..12240
                     /locus_tag="WGR74_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
BASE COUNT         3671 a         2835 c         3132 g         3282 t
ORIGIN      
        1 tttttgacac attttcatga tttcaaatcc cgttattccc aagtggacgg aaacccgata
       61 atgggaagcc gcagagactt atcgggaaaa cggcaagccc tccgccgtca ttcccgcgca
      121 ggcgggaatc tagaaataaa aaaccacggt gttatcggca atgactgaaa ccggagaaat
      181 gacgaaattg aagcgtgcgg aaatgtgtcg gaaatcgctg aagccggata aactagattc
      241 ccgcttttgc gggaatgacg ggattttagg tttttggttt gttttcacgg caatgatggg
      301 attttaaatt gcaggtattt accgggaaaa acggcaagct ctccgccgtc attcccgcgc
      361 aggcgggaat ctagaaatga aaaaccacgg tgttatcgga aatgattgaa accggagaaa
      421 tgacggaatc gaagcgtgtg gaaatgtgtc ggaaatcgct gaagccggat aaactagatt
      481 cccgcttttg cgggaatgac gggattttag gtttttggtt tgttttcgcg gcaatgacgg
      541 gattttaggt ttttggtttg ttttcacggc aatgatggga ttttaaattg caggtattta
      601 ccgggaaaaa cggcaagccc tccgccgtca ttcccgcgca ggcgggaatc tagaaatgaa
      661 aaaccacggt gttatcggca atgactgaaa ccggagaaat gacgaaattg aagcgtgtgg
      721 aaatgtgtcg gaaatcgctg aagccggata aactatagtg gattaaattt aaatcaggac
      781 aaggcgacga agccgcagac agtacagata gtacggcaag gcgaggcaac gctgtactgg
      841 tttaaattta atccactata gattcccgct tttgcgggaa tgacggtatt ttagattttt
      901 gttttcgcgg aataatgagc caaaagccgt ccgaacccaa aaaaaccgtc gctttcagac
      961 ggcatcaaaa aaccgcaggc acgatgcctg cggtttgccg tttgcgcttc aggggagcag
     1021 ggggattgcg cccaagccgt gtgtttcctt cagtccgaac atgatgttca tattttgcac
     1081 cgcctgaccc gccgcgcctt tgacgaggtt gtcgatgacg gaaaggacga cccacacatc
     1141 ggattgcgcc gcctgttgga tgctgatgcg gcagaggttt gcgccgcgca cgctgcgggt
     1201 ttcgggtgtc gaaccggcgg gcaggatgtc cacgaacagg ctgtcgcggt aatagtcgcg
     1261 caggatggtt tcggggcaga tgccgtctga aaggtggagg taaacggtgg cgtgcatacc
     1321 gcgtatcatc ggcgcgaggt gcggcgtgaa cacgaatcct tcggcgatgc cgtcctgaag
     1381 cccggcgatg gtctgcctga tttcgggcag gtggcggtgt ccagctatgc cgtatgcttt
     1441 gaagttgtcg ccgacttcgc acaacagcga accgacattg cctttcctgc ccgcgccgga
     1501 cacgccggat ttgcagtcgg caatcagcgg cataccgggc ttcagacggc attgccgcaa
     1561 cagcggcacg agcggcaggg atacgcaggt cgggtagcag ccggggttgg cgacgaggcg
     1621 tgcctgtgcg acggcttcgc ggttgagttc gctcaatccg tacacggctt gggaaacgag
     1681 gtcgggggcg gcgtgggtca tgccgtacca gtgttcccag gtcggaatgt cccgtatgcg
     1741 gaagtcggcg gaaaggtcga tgacgcgcac gccctgttca atcaggcgcg gcgcgtcttt
     1801 catggcgatg ccgttgggcg tggcgaagaa gacgatgtcg cattgttcca aacctgcctc
     1861 gtcgggcgtt tggaaggcga ggccgtacac gccgcgcaaa ctcggaaagt aatcggcaac
     1921 tgcagttccc gcttcgctgc ggctggttac ggcggcgact tcgacatcgg gatgggcggc
     1981 aagcaggcgc agcagttcca cgcccgtgta gcccgtcgcg ccgacaatgc cgactttgat
     2041 ttttttactc atggtttttc ctttgtgtgg ttggcgggta tgccgtccga acgctgtccg
     2101 acggcttgtc cgacggggtt ttgattggcg gcggaaggac ggtacacaaa gcgtttgaaa
     2161 agcgtgtcta atttaatggc atccgcctct gtttttaaaa cccagccctg ccgtccggaa
     2221 taaacataac cgtaggcgcg aaagtcggca ggatatttcg gtgcctgccc caaacccaaa
     2281 ccgtatgcgg cgaaagcggt ggtcgtggag aaggaaagca ggaggaggac gagggttttc
     2341 ataaggttcc aaaccgaacg gggcgatgaa gcccgattat agcaaaaggt cgtctgaaaa
     2401 ccgttttcag acgacctttt atttaattac aataaaaatg ttaacaacaa aaaacaaacc
     2461 gcttttttcc gtttggagac aaatttttaa ctaaaattgt caacaattcc ttgacgcaca
     2521 cacaaaaaaa acttcaatat ttgtaaatat atgtaacatt ataaatttta aatattttct
     2581 gagtaagggg aagtaatgag catccatctc gacttcggca ttagtcctaa aacgttccga
     2641 cagacttatc tgtatcaaaa gcccaagctc tttaaaggag cggttcggaa tctcgaatcc
     2701 gcatcttgga aagatatcaa cgagatatac caacgagcag acccaaccgc accgctgttt
     2761 catctgcgta aaaaaggcgc aatcgttcct aaagaagaat acgtcgaaag tttcgacgat
     2821 ttgggcaaaa ctcgctaccg ttttattaaa tccgttatct acgaacatat gaagaatggt
     2881 gcgtcgttag tctataacca tattaacaac gagccgtttt cagaccatat cgcccgtcaa
     2941 gtcgcccgct ttgccggcgc acatactatt gttagtggat atcttgcttt tggcagcgac
     3001 gaatcttata aaaaccattg ggacacccgc gatgtgtatg ccatccagct tttcggcaag
     3061 aaacgttggc aacttactgc ccctgatttc cctatgccat tgtatatgca acagactaaa
     3121 gatactgata tttccattcc tgaacatatc gatatggata ttatccttga agcaggtgat
     3181 gtcctctaca tcccgcgcgg ttggtggcaa agacctatcc cgctcggctg tgaaaccttc
     3241 cacctcgctg tcggtacctt tccgcccaac ggctataatt acctcgagtg gctaatgaag
     3301 aaattcccca ccatagaaag tctgcgccac agtttctcag actgggagca agataggacg
     3361 cgtatcaacg atactgccgc acaaattgct gccatgattg ccgaccccgt caattacgaa
     3421 gccttcagtg aagacttcct cggcaaagaa cgtaccgata ccgcttttca tctcgaacag
     3481 ttcgcgaatc ccaacgctac tccgctttca gacgacgtca ggttgagact aaatgccaat
     3541 aatttggata cgttggaaaa gggatatttg attgggaatg ggatgaagat aagcgtagat
     3601 gagttgggga aaaaagtgtt agaacatatc ggtaagaatg aaccgttatt gttgaaaaat
     3661 ctactggtta acttcaatca ggaaaaacat gaagaagtta agaagttgat ttatcagttg
     3721 atagagttag attttctgga acttttgtga gggattctat gaaaaactgg aagcaattta
     3781 catttttcgt aatattagta atagcttgtt atcaattgct atatttttta tcagatatgt
     3841 ttcttctcga ctacattaac aaatatagtt ggaatttgaa ttttattcag ggtactttga
     3901 attttttttc aatatatctg ccatatgttt ttgttagccg aatttttagg aataccaacc
     3961 aatgaaagac taggctatga aatcaaaatt tgtgtataag ttactggtgc ttgcaaagta
     4021 cctgtatatt ttaatttgta tatcaatacc aatcgttttt atttatgaaa gctaccaagg
     4081 agatgatgtt aattttatga tttatattcg tgcttttatt attttttcca tattgggtgt
     4141 attgactcaa tattctatgt ctaatttttt tgaaaaggaa aaataacatc attgaactat
     4201 ccattgagaa tctggagcta atttatggcg gaggcttagg ggagagaatc cttgaaggag
     4261 gagcaaatgg tgcacaaata ggaggatcaa ttggtgcagg tattggtctg ggtgtcggta
     4321 tagtttctgg tggtattcct gccggaccgg ctattattct cggtggcaca gttggtgctg
     4381 ctgttggtgc tgtaactggt atgggtgttg cagcatatga gtattaccaa gaaactcagc
     4441 ccaaagggac agtaacggtc ggtccgattt ctgcttttgc tgtggacaaa tcaggaaacg
     4501 gctacactga cagcacaaat tactgctgat atttctcatc gtccagacaa tctctagggg
     4561 tcgtctgaaa ctttcttaac ttcaatttta tgaatagacc caagcaaccc ttcttccgtc
     4621 ccgaagtcgc cgttgcccgc caaaccagcc tgacgggtaa agtgattctg acacgaccgt
     4681 tgtcattttc cctatggacg acatttgcat cgatatctgc gttattgatt atcctgtttt
     4741 tgatatttgg taactatacg cgaaagacaa cagcggaggg acaaatttta cctgcatcgg
     4801 gcgtaatcag ggtgtatgca ctggatacgg ggacaattac agcgaaattc gtggaagatg
     4861 gagaaaaggt taaggctggc gacaagctat ttgcgctttc gacctcacgt ttcggcgcag
     4921 gaggtagcgt gcagcagcag ttgaaaacgg aggcagtttt gaagaaaacg ttggcagaac
     4981 aggaactggg tcgtctgaag ctgatacacg ggaatgaaac gcgcagcctt aaagcaactg
     5041 tcgaacgttt ggaaaaccag gaactccata tttcgcaaca gatagacggt cagaaaaggc
     5101 gcattagact tgcggaagaa atgttgcaga aatatcgttt cctatccgcc aatgatgcag
     5161 tgccaaaaca agaaatgatg aatgtcaagg cagagctttt agagcagaaa gccaaacttg
     5221 atgcctaccg ccgagaagaa gtcgggctgc ttcaggaaat ccgcacgcag aatctgacat
     5281 tggccagcct cccccaagcg gcatgagaca gaacaaagct ggcttgaacg caccattgcc
     5341 gatatttctc aagaagtttt ggattttgaa atgcgctctg aacaaatcat ccgtgcagga
     5401 cggtcgggtt atatagcaat accgaacgtc gaagtcggac agcaggttga tccttccaaa
     5461 ctgctcttga gcattgttcc cgaacgtacc gagctatatg cccatctata tatccccagc
     5521 agtgcagcag gctttatcaa gccgaaagac aaggttgtcc tacgttatca ggcatatccc
     5581 tatcaaaaat tcgggcttgc ttccggcagt gtcgtatcag tagcaaaaac ggcactgggc
     5641 agacaggaat tgtcgggatt gggcatggta tcctccgatt tggcgaagag caacgaacct
     5701 gtttatctcg tgaaaataaa acccgacaaa ccaaccatca ctgcatacgg tgaggaaaaa
     5761 ccgctgcaaa tcggcatgac gttggaagca gacatcctac acgagaaacg gcgattgtac
     5821 gaatgagacc tttgcaataa cataggttac taaaatttta tgctcaatct cattttcaaa
     5881 atgcaaaact tttctgattt ttcctacttt ttgctcaata ttaggaaggt tttaggcaat
     5941 tgaaaatttt ttggcgcatt tttatgcgtc aaatttcgtt aacagactat ttttgcaaag
     6001 gtctcgaatg ggtattggag ccgatttata gtatatcggg caaactgtaa gacttgaggc
     6061 tgaaagataa agaagttgtc tgaaaccttt tcagacgacc agaagacaat atataaaaac
     6121 acaaacttca aaactgcctg aaaccatccc cttttacagg cactccgttt gagacctttg
     6181 caataacata ggttactaaa attttatgct caatctcatt ttcaaaatgc aaaacttttc
     6241 tgatttttcc tactttttgc tcaatattag gaaggtttta ggcaattgaa aattttttgg
     6301 cgcattttta tgcgtcaaat ttcgttaaca gactattttt gcaaaggtct ccgtctgtaa
     6361 aagcaaggat agggcatctg cccttttgat tgtttgatta acgatacaag gagtttcaaa
     6421 atgagagttt tatagtggat taacaaaaac cagtacagcg ttgcctcgcc ttgccgtact
     6481 atttgtactg tctgcggctt cgtcgccttg tcctgattta aatttaatcc actatatgtg
     6541 ttcatgaaat gacttgggtc ggaggctcag gtaatgcgca acaaagttca tattattgcg
     6601 aaatttgcga atctgcaggg cttaacgata cgggaaatcc tgataaatct ttaggattgc
     6661 caaacaatac gttcagtaat ccgcctggtt ggggagctac aatcggagct ttagcaggta
     6721 gccgcatagg gatgcctgaa tttggtacgt ttgcgagcca tgccattgaa aatttcgact
     6781 ggtcatggta tcgacgttat agggaaattg ccgaaacgat tgaacgagaa tattcaggcg
     6841 gtttgcctta atagttgagg aggtcatgat gtttgccaaa cattatcaat tcatcgcact
     6901 cggcatcatg ctgcttcttt atatgttgat tctctatacg accgattttt ccaatctgac
     6961 gtattggatg ctgtttttta tctgttttat tacaggaaaa atattagctc gtttgttaga
     7021 gaaaagcttt aaataaaata gcagctagtc gcaaaaggtc gtctgaaacc ttttcaggcg
     7081 gcctttctaa aatacatcca acttcctaat ccctattttt caaaaaggaa atctatgccc
     7141 catctgcaaa acctgtcttt gggcttaaag aaaaagctgc ctgttatcct gcaaacagaa
     7201 atatcagaat gcggcttggc atgtctggcg gctgtggcgg gatttcatgg tttccatacg
     7261 aatttacgcg cactgcgttc aaaatactgt ccgagacctt tgcaaaattc cccaaaatcc
     7321 cctaaatgtc ttggtgggaa ttttggggaa ttttgcaaag gtctcattct ataactgtaa
     7381 atacttttaa atttatgaca aaatagtaaa tattgctaaa ataatattga tgtcatgaaa
     7441 ttttttcctg ctccatgtct gttggttatc ctggctgtca taccccttaa aaccttagct
     7501 gccgatgaaa acgatgcaga acttatccgt tccatgcagc gtcagcagca catagatgct
     7561 gaattgttaa ctgatgcaaa tgtccgtttc gagcaaccat tggagaagaa caattatgtc
     7621 ctgagtgaag atgaaacacc gtgtactcgg gtaaattaca ttagtttaga tgataagacg
     7681 gtgcgcaaat tttcttttct tccttctgtg ctcatgaaag aaacagcttt taaaactggg
     7741 atgtgtttag gttccaataa tttgagcagg ctacaaaaag ctgcgcaaca gatactgatc
     7801 gtgcgtggct acctcacttc ccaagctatt atccaaccac agaatatgga ttcgggaatt
     7861 ctgaaattac gggtatcagc aggcgaaata ggggatatcc gctatgaaga aaaacgggat
     7921 gggaagtctg ccgagggcag tattagtgca ttcaataaca aatttccctt atataggaac
     7981 aaaattctca atcttcgcga tgtagagcag ggcttggaaa acctgcgtcg tttgtcgagt
     8041 gttaaaacag atattcagat tataccgtcc gaagaagaag aaggcaaaag cgatttacag
     8101 atcaaatggc agcagaataa acccatacgg ttcagtatcg gtatagatga tgcgggcggc
     8161 aaaacgaccg gcaaatatca aggaaatgtc gctttatcgt tcgataaccc tttgggctta
     8221 agcgatttgt tttatgtttc atatggacgc ggtttggtgc acaaaacgga cttgactgat
     8281 gccaccggta cggaaactga aagcggatcc agaagttaca gcgtgcatta ttcggtgccc
     8341 gtaaaaaaat ggctgttttc ttttaatcac aatggacatc gttaccacga agcaaccgaa
     8401 ggctattccg tcaattacga ttacaacggc aaacaatatc agagcagcct ggccgccgag
     8461 cgcatgcttt ggcgtaacag gtttcataaa acttcagtcg gaatgaaatt atggacacgc
     8521 caaacctata aatacatcga cgatgccgaa atcgaagtgc aacgccgccg ctctgcaggc
     8581 tgggaagccg aattgcgcca ccgtgcttac ctcaaccgtt ggcagcttga cggcaagttg
     8641 tcttacaaac gcgggaccgg catgcgccaa agtatgcccg cacctgaaga aaacggcggc
     8701 gatattcttc caggtacatc tcgtatgaaa atcattactg ccggtttgga cgcagccgcc
     8761 ccatttattt taggcaaaca gcagtttttc tacgcaaccg ccattcaagc tcaatggaac
     8821 aaaacgcctt tggttgccca agacaagttg tctatcggca gccgctacac cgttcgagga
     8881 tttgacggtg aacagagcct ttccggagag cgaggtttct actggcagaa cactttgggc
     8941 tggcatttcc atccgcgcca tcaattctat ctcggcgcgg actatggtcg ggtatcgggc
     9001 gaaagtgccc aatatgcctc gggcaagcgg ctggcgggtg cagtggccgg cttcagaggc
     9061 agccataaag taggcggtat atttgcttat gacttatttg ccggcaagcc gctgcacaag
     9121 cccgaaggtt ttcagacggc caacaccgtt tacggcttca atttgaatta cagtttctaa
     9181 cctctgaatt ttttactgat atttagacgg tctttcctta tcctcagacc gtcaaacttt
     9241 acctacgtac ttggcgcgca gtacgttcat cttcaaaatg gaatagacat gaataaaggt
     9301 ttacatcgca ttatctttag taaaaagcac agcaccatgg ttgcagtagc cgaaactgcc
     9361 aacagccagg gcaaaggtaa acaggcaggc agttcggttt ctgtttcact gaaaacttca
     9421 ggcgaccttt gcggcaaact caaaaccacc cttaaaacct tggtctgctc tttggtttcc
     9481 ctgagtatgg tattgcctgc ccatgcccaa atcaccaccg acaaatcagc acctaaaaac
     9541 cagcaggtcg ttatccttaa aaccaacact ggtgccccct tggtgaatat tcaaactccg
     9601 aatggacgcg gattgagcca caaccgctat acgcagtttg atgttgacaa caaaggggca
     9661 gtgttaaaca acgaccgtaa caataatccg tttctggtca aaggcagtgc gcaattgatt
     9721 ttgaacgagg tacgcggtac ggctagcaaa ctcaacggca tcgttaccgt aggcggtcaa
     9781 aaggccgacg tgattattgc caaccccaac ggcattaccg ttaatggcgg cggctttaaa
     9841 aatgtcggtc ggggcatctt aaccaccggt gcgccccaaa tcggcaaaga cggtgcactg
     9901 acaggatttg atgtgcgtca aggcacattg accgtaggag cagcaggttg gaatgataaa
     9961 ggcggagccg actacaccgg ggtacttgct cgtgcagttg ctttgcaggg gaaattacag
    10021 ggtaaaaaac tggcggtttc taccggttct cagaaagtag attacgccag cggcgaaatc
    10081 agtgcaggta cggcagcggg tacgaaaccg actattgccc ttgatactgc cgcactgggc
    10141 ggtatgtacg cagacagcat cacactgatt gccaatgaaa aaggcgtagg cgtcaaaaat
    10201 gccggcacac tcgaagcggc caagcaattg attgtgactt cgtcaggccg cattgaaaac
    10261 agcggccgca tcgccaccac tgccgacagc actgaagcat caccgactta tctctccatc
    10321 gaaaccaccg aaacaggcgc agcaggcgca tttatctcca atggtggtcg gatcgagagc
    10381 aaaggcttat tggttattga gacgggagaa aatatcagct tgcgtaacgg agccgtggtg
    10441 cagaataacg gcagtcgccc agctaccacg gtattaaatg ctggtcataa tttggtgatt
    10501 gagagtaaaa ctaatgtgaa caatgccaaa ggctcggcta ctctgtcggc cggcggtcgt
    10561 actacgatca atgatgctac tattcaagcg ggcagttccg tgtacagctc caccaaaggc
    10621 gatactgaat tgggtgaaaa tacccgtatt attgctgaaa acgtaaccgt attgtccaac
    10681 ggcagtattg gcagtgctgc tgtaattgag gctaaagaca ctgcacacat tgaagcgggt
    10741 aaaccgcttt ctttagagac ttcaaacgtt gcttctaaca tccgtttgaa caacggtagc
    10801 attaaaggcg gaaagcaggt ggtattgatg gctgatgacg acattcaggc aaaagcctcc
    10861 cacctgaatg cgtccggcaa tctgtatatt catgcaggta aggatctgga tttgaatgcc
    10921 gataaagact tatcaacaca aagtatcagc ctgagggcag acaacaccgc cctcatcagc
    10981 agcaacggta acaccttgac tgcagaaaag aatctggata ttcaggcagg tagcttgagt
    11041 gtgcgtcaaa gtaatctgca atccagtggc ggtaatgtgc agatgagcgc gactaaaggc
    11101 aacatcagct tgaatcagag ttggataaat gccagtcaga atattgatac ggcagcactt
    11161 caaggcaata ttatttcaga cggtctgact gctgttgccg aagttggacg cgtatctctt
    11221 cttgccaacg gcaatgttga tttcaacggt ctaaatacct tgattgcgga aggagacatt
    11281 aatgctggtt cagtcggtaa aggccgtctg aaaatggaca ataccgatat ttatgcttct
    11341 gcaggcgatg tgaaactggt tgccggaggt caattagatt taggcaacgg caccgttaac
    11401 ggcggtcata tcagcttaga cagcaataaa ggcagcatgg tggtgcagaa tgtacacctg
    11461 aatgcccgcg catcactgaa agtggatgcg gatcaaactt taaccattaa taacagcaag
    11521 ctcaattccg atcacaatac ccagattaat acaaatcatg gtcatatgac gcttaatcag
    11581 cttgatgctc attcacgtcg tcatatgagt atcagtgcac agggtaaagg caaaggaaaa
    11641 gacagcggtc aaattttaca aaacgaccag caaaacagta aaagtacttt ggcggcagat
    11701 ggtgtattgt cattgaacag cagcgcatta caggttttgg acaatactac cctgcgcggt
    11761 ggcgcgataa acatcaaagc tggcggaggc atcatcaaac gggggcatat cgattgggaa
    11821 acccaagata cggcaactat gcgttcagcg gaactgaaac cactgtccgg tatgatgtcg
    11881 atagaatcgg gcggtgataa ccctttaacc gttgaacccg gtaaccgtat cgtttctgca
    11941 ggtgatttgg ctgtgaaaca caacggcaca ttccaaatca gtgccagagc aggaaataac
    12001 ggcaatccga gtgcgcaaac agccagcgtt tcagctaaag gcaatatcgg gattgtggcg
    12061 ggagaggtgg atattgatgc agcaaatatt gccgcaggga aagatttggc tttagtggca
    12121 actaaaggga atatttcact aaacagtatt aggaatacgt ttagtaacta tcaattgaaa
    12181 acagataaac acaatatcac acagcaactt actgatgtag agcaggagct tagtaagtta
    12241 acgagcgatc ctaaatatcg taaagcacag gatcttccac aaatgttgag gcgtaaatat
    12301 aagaggcgtg acaaagtatt cggtgatagt gaggccagat tacgtggttt acgagcaaag
    12361 ataaatgctg ctgatgaggc ttgggcagaa cgccaatccc cagtaaaagc gttacttgaa
    12421 cgtaaacaat tgttacaaca agccctttta acagtttccc aaccgggtag tggtcacgaa
    12481 aaccaaggta gcacccttag cggtcaaaat atcaaactgc tcgctgcagg tggtatccgt
    12541 atccagggct ccaaagttgc tgccacccaa caggcaaata tccaagctgc aggattttta
    12601 cctgcaccag ccgcagagga actgcaagaa ggtcgacttc aatcagccat tgacatcagc
    12661 ggtgtacttg atacttttga atatggtcag caaggcagcg ataaatacgg ctatgccatt
    12721 ttcagcaggc catctgaaat ttcaggaaaa acaggtgtta ccctctctgc accaaatgcc
    12781 aatgagaaca gccgcatcag tctgagtgcg gcaaacatcg aagctgaaaa cggcaaaatc
    12841 aaaattcaat cttacggtga ccaatattac tatgcgagac agagcgaact ctataccttt
    12901 gaacgccgca gctacaaaac
//
LOCUS       DAWFLT010000057        12834 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWFLT010000057 DAWFLT010000000
VERSION     DAWFLT010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12834
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.057"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(963..1631)
                     /locus_tag="WGR74_001512"
     CDS             complement(963..1631)
                     /locus_tag="WGR74_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0905904.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCHLFGITNIIGTQLETGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVDEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            complement(1628..2296)
                     /gene="hda"
                     /locus_tag="WGR74_001513"
     CDS             complement(1628..2296)
                     /gene="hda"
                     /locus_tag="WGR74_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ0905905.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            complement(2401..2979)
                     /locus_tag="WGR74_001514"
     CDS             complement(2401..2979)
                     /locus_tag="WGR74_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0905906.1"
                     /translation="MFRHNGEILLSGINIKLKSGDSLLIRGPSGCGKTSLLRALAGLW
                     PFGSSGKVSRPPHQDILFLPQRPYTAQGSLRDAVCYPDIDKQHPELIEAMNTCRLGYL
                     IDKLDKTDDWQHKLSLGELQRVAFVRALLSKPKIVLLDEATAALDEPAEAALYRALKQ
                     KLPDSIIISIGHRSTLNAFHNMRLDVGNVACG"
     gene            complement(3201..3413)
                     /locus_tag="WGR74_001515"
     CDS             complement(3201..3413)
                     /locus_tag="WGR74_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0905907.1"
                     /translation="MNHAETEPRDGLYVLRIGNDLFVKRVQRIPGKLLVTSENPRYAP
                     FEIDLSNTQDDIAIIGRVEWYGRSID"
     gene            complement(3580..4293)
                     /locus_tag="WGR74_001516"
     CDS             complement(3580..4293)
                     /locus_tag="WGR74_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEZ0905908.1"
                     /translation="MRLYESGQVNIPADFLLAAVQLGVDIQFVFTGIHSANLNAVSDD
                     LNKKLDDAIGKSNVENQINGNVSNAVIASSGSVVHQINTQNHVIRTRVDSKPGKEHIT
                     LEQASKLQQLVKQVAAAEEIAKRSPKSIRAIWASLNAHCKVPSYKLIALSDYDKAETY
                     LRKWLGRLSNTATSKNNDPDWRKKKYAYIKLNVKQLELEDWLKSYLEKNFAVESLTEL
                     SNDDLQKTYAAVSTKKRKK"
     gene            4526..4777
                     /locus_tag="WGR74_001517"
     CDS             4526..4777
                     /locus_tag="WGR74_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0905909.1"
                     /translation="MQANEIYKSLEEKNISARMLASALGVTNQSVSEVIRNGRGSKRI
                     AEAIAKVIQKDLVEVFPHYKKTDCRDKKIAELKEMLGVG"
     gene            4779..6756
                     /locus_tag="WGR74_001518"
                     /pseudo
     CDS             4779..6756
                     /locus_tag="WGR74_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6823..6900)
                     /locus_tag="WGR74_001519"
                     /pseudo
     CDS             complement(6823..6900)
                     /locus_tag="WGR74_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            6971..7885
                     /locus_tag="WGR74_001520"
     CDS             6971..7885
                     /locus_tag="WGR74_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEZ0905910.1"
                     /translation="MKQINQALQQKLVEFKEKSGMNQTQLARGIDTSPASISMYLNGT
                     YADKGGNYETIETKIEAFLEMQDSKARREELVLGFVSTKTTRRIAEAMRDAHEGGEIV
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSLPLNLGESLPDEELFEIAKAALPDADEETLLELVKHSNGNTRRMSKLM
                     RGAVRTANKNGIKMQAGIVKKYSSLIIR"
     gene            7976..8242
                     /locus_tag="WGR74_001521"
     CDS             7976..8242
                     /locus_tag="WGR74_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905911.1"
                     /translation="MQVLKKVDWKMFVARSFWRCVPVGLTAGVWCFVAGMALHSCAKE
                     PEPVAKELTKVEKMERQADLEVKVWEEQYESMSVEEKMKGIVYE"
     gene            8351..8524
                     /locus_tag="WGR74_001522"
     CDS             8351..8524
                     /locus_tag="WGR74_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905912.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATARMAQKSREREIEEYQAQFEDKD
                     GKGRLKTGGNKDE"
     gene            8517..8684
                     /locus_tag="WGR74_001523"
     CDS             8517..8684
                     /locus_tag="WGR74_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905913.1"
                     /translation="MNEKDLIEWLEDRGELMVMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            8748..8975
                     /locus_tag="WGR74_001524"
     CDS             8748..8975
                     /locus_tag="WGR74_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905914.1"
                     /translation="MKVYVFKISNENGKLKIELPEIPMGKQIDEVDLIAGLTTEFIAS
                     MLRDAQKDRRKFVIDASNQLAAIQTYQKIFN"
     gene            8990..9508
                     /locus_tag="WGR74_001525"
     CDS             8990..9508
                     /locus_tag="WGR74_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEZ0905915.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAAIEEEYAAKAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDAILNQKERFSDGQVPGIKIVSGV
                     EDFVIEPSEQIL"
     gene            complement(9668..9883)
                     /locus_tag="WGR74_001526"
     CDS             complement(9668..9883)
                     /locus_tag="WGR74_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905916.1"
                     /translation="MNTTYTLTFDQDSLKALNLLVFTLNHLKVLDMDMEGIEDGLSAV
                     IETASEKADKLSAAFYNAVYEQEEKAA"
     gene            complement(9887..10485)
                     /locus_tag="WGR74_001527"
                     /pseudo
     CDS             complement(9887..10485)
                     /locus_tag="WGR74_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
     gene            complement(10617..10817)
                     /locus_tag="WGR74_001528"
     CDS             complement(10617..10817)
                     /locus_tag="WGR74_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905917.1"
                     /translation="MDSIKKAQEKYALKRVVKTVSFNSEKELDLIKFSENLDFSNWVK
                     SKIRGDMESEKFKKKAKKNIDK"
     gene            complement(11018..11203)
                     /locus_tag="WGR74_001529"
     CDS             complement(11018..11203)
                     /locus_tag="WGR74_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905918.1"
                     /translation="MPDEKTLKAKQAEYQRKYDQKRLMKTVSFHLEREKELVDFMQKE
                     IPDFSNWVKSVMKEKMK"
     gene            complement(11476..11643)
                     /locus_tag="WGR74_001530"
     CDS             complement(11476..11643)
                     /locus_tag="WGR74_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905919.1"
                     /translation="MVDEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNIDFSQW
                     VKSIIKEKIKK"
     gene            11880..12122
                     /locus_tag="WGR74_001531"
     CDS             11880..12122
                     /locus_tag="WGR74_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905920.1"
                     /translation="MDTLKVNPFPNKIKEKCRLKTARMLFQTAFFVVGHQCPAICAAL
                     RLSRYGILVCIFRRNLPYIVLKYYTVVLYGFDFLEK"
     gene            12166..12357
                     /locus_tag="WGR74_001532"
     CDS             12166..12357
                     /locus_tag="WGR74_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905921.1"
                     /translation="MNTVKEYTAVRERLLNAADYLEEVRKDRKTGNIASVEFVPPKIG
                     ARGYGKFKVRYKTLVAVDL"
     gene            12354..12770
                     /locus_tag="WGR74_001533"
     CDS             12354..12770
                     /locus_tag="WGR74_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905922.1"
                     /translation="MSGEKSENLPADEQQKMLQEFLELQKQDIAVKQKELESRKDEIQ
                     SHERIALATIEAQKQGEQQHGEIFKEVHKRRLNAVIAISFLIAAVLITALWRDKDSVA
                     IEIVKIGGAVALGYFAGINRGKAQTLEKQRREKDSD"
BASE COUNT         3428 a         2575 c         3377 g         3454 t
ORIGIN      
        1 gtcgccttgt cctgattttt gttaatccac tatacgtcct agattcccac tttcgtggga
       61 atgacgggat gtgggttttt gtgcggattt gaaccggtaa gggtggtgtg ggattggtgg
      121 tttgcttagg atcttttgga ttgtattttg tatatacatt tacttgttga taaaagataa
      181 aataaaatta gaaactaaaa gtgagaaaaa attaataata atagggatgt ataaatgtaa
      241 aggctccgtt tcatagctaa ggttatctga atatatggaa aaaaagtaaa agtccataaa
      301 actaaaatat atagataatg ctaatgataa tagaattatt actttcctag agtaagggta
      361 atattttttt atcgtaaaaa aatataaaat aggataaata attgccatat agatagattt
      421 tgtaacaaaa ttaaaaataa ataataaaaa taatgtaata aagtatattt tttggctatg
      481 aaataaaatt gtacataatt gaacgagcag atcaaaaaat gaactacata taacaataaa
      541 taataacgta tttaccatac taaatttaat aggtctcatt atcatattta ataaccactt
      601 catagtaaat gcagagtggg tggaaacact cactttatgg tttgctacgc tctgttcaat
      661 tagcaacccg ataacccaat atagataata gggtaattaa tctaatctaa tttgtcagca
      721 tccgttaatt tattgcaaaa taaagtattg aattatgtcg ggtgcaaatg acgaaatata
      781 agtttccgtg cggacggatc aagattccca ctttcgtggg aatgacggtg gaaagattgt
      841 tgtttttccc gatgaattcc tgtgtttttt gtttttccgg ataaattcct gtggctttga
      901 gttttttgga tttcagcctc aatgccgtct gaacgccgaa tcgggcttca gacggcattg
      961 cgtcatttga aattcaaaac cggccagcct ttttctttgg cttctttttc cagctcggca
     1021 tcggggttga cggcgacggg ttcgtcgacg aggcgcagca gcggcaggtc gtttttggag
     1081 tcgctgtaaa aataggtttt gccgtagctt tcgagcgttt cgccgcgttc ggcgagccat
     1141 tggttcaggc gggtgatttt gccttctttg aggctgggcg tgccgatgta attgccggtg
     1201 tagcggccgt cagaaccggt ttcgagttgt gtgccgatga tgttggtgat gccgaaaagg
     1261 tggcagacgg gggtgatgat gaactcgttg gttgaggaaa tcacaagggt ttcgtcgcct
     1321 gccatttggt ggctctgcac cagcatacgc tgcataggcg agatgtgggg gatgatgtat
     1381 tccgccataa attcgcggtg aaactctgcc agctcttctt tgctgtaacg agcgagcggg
     1441 gcaaggtgga atttgaggaa tgcgtcgatg tcgaggcaac cgttttggta gtcgcggtag
     1501 aatttttcgt tttgcgcttc ggtttcggca gcgtcaacca agcctttttt gatgaggtat
     1561 tgcggccagg cgtggtcgga atcggtgttg atgagggtgt tgtcgaggtc gaagatggcg
     1621 aggtttttca ttgggtttcc tgttgtttca aaagctggcg caaaagcggc agggtgatgc
     1681 gtttgcccat tgtgacggcg tagttgtcca gcgtgtcgag catcatcatc aggctgtcca
     1741 tatcgcgccg ccagtgtttg agcaggtatt cgaaaatttc ggaatcgacg gttacttggc
     1801 gtgccgccgc catactggcg agcgcgtcga ttttttcttg gtcggttaag ggcttgactt
     1861 cgtaaacgag gcagtacgcc atacgcgtcc gcaaatcttc gcggatgacg agctgctggg
     1921 gcgtgtattc cgaaccgagc agcaaaaagc ctttgccgct gttgcggaag cggttgaaga
     1981 tggaaaaaag cagggcttgt tcttcgttgc ccagtttttc gacttgatcg acggcgaggt
     2041 attccgcctc gaacgcggca tcggtcagcg gcatggaggc ggcatcgata taggcggcgt
     2101 ttttgccggc ttcgagcgcc tgtgcgaccc acgcctgcaa aagatggctt ttgcccgcac
     2161 cttcttcacc ccagacatag ataaactgtc cgtgtttgtg tcggaggaca tagaccagtt
     2221 ccgcgttttc cgtgccgagg aatttgtcga aactcggata gtcgtgtgcg gcaaagtcga
     2281 aaataagctg gttcacggtt cggcattccg aggggtggta aacgggttta ttgtacgttg
     2341 ttttcgcgtg ctttatcaat ttgaacgatg ccgtctgaaa acggcttcag acggcatcgt
     2401 tcaaccgcag gcaacgttgc cgacatcgag gcgcatattg tggaacgcgt tgagcgtgct
     2461 tcggtgtccg atgctgatga tgatgctgtc gggcagtttt tgctttaagg cgcggtagag
     2521 cgcggcttcg gccggttcgt ccaaggcggc ggtggcttcg tcgagcagga cgattttggg
     2581 cttggaaagc agggcgcgga cgaaggcgac gcgttgcagt tcgcccaggg agagtttgtg
     2641 ctgccagtcg tctgttttgt ccaatttgtc aatcagataa cccaagcggc aggtgttcat
     2701 ggcttcgatt agctccggat gctgtttgtc gatgtcggga taacaaaccg cgtcgcgcag
     2761 gctgccctgt gccgtgtacg ggcgttgcgg caggaagagg atgtcttgat gcggcggacg
     2821 gctgactttg ccgctgctgc cgaacggcca aagcccagcc agcgcgcgca gcaacgaagt
     2881 tttgccgcaa ccgctcgggc cgcgtatcag cagggaatcg ccgcttttga gtttgatgtt
     2941 gatgccgctc aacaggattt cgccgttgtg gcggaacagg gcgacgtttt cctgtggggg
     3001 actgttagtt tttgcacaag gaacaaatag agtaaaaaaa cgctgaaatc ttcggaagac
     3061 gtggatttcg gcgttttttt gtatccggaa aagttacgcc agctttttca caaaaccgcg
     3121 ccggaatgcg cggttttctg tttaaagctg acgagattag ggaattttta aaactgtttt
     3181 aagaggtttt taaaatggat ttaatcaata ctccggccat accactcaac acggcctatg
     3241 atggcgatgt cgtcttgggt attactcaaa tctatttcaa acggtgcgta acgtggattt
     3301 tcagacgtta caagcagttt gcccggtata cgttgcacac gtttgacaaa gaggtcattg
     3361 cctatacgca agacatatag gccgtcgcgc ggctcggttt cggcgtggtt gattagaatg
     3421 tttataaaag gctttaacta gaaactgaac atggaacttg tataatagtt ccatgtttga
     3481 aaaaatcttt taatatcata catctaaaaa aacaaaacat ctgaagacca taagctaaac
     3541 atgaaacttg aatttaaagg cggagtttca tgtttaagcc tattttttcc gttttttagt
     3601 cgatactgct gcatatgttt tttgcaaatc gtcattactt aattcagtca ggctttctac
     3661 tgcgaaattt ttttcgaggt aacttttcaa ccaatcttca agctcaagtt gttttacatt
     3721 taatttgata taagcgtatt tcttttttcg ccagtcggga tcgttgttct ttgatgttgc
     3781 tgtattgctc aacctaccca gccattttct gagataagtt tctgctttgt cgtaatcgct
     3841 caatgcgatt agtttgtaac ttggaacttt acagtgagca ttcagactag cccaaattgc
     3901 acggattgat tttggcgacc gtttggcaat ctcttctgct gcggcaactt gtttaacaag
     3961 ttgttgcagc tttgaagctt gctcaagcgt tatatgttcc ttgccgggtt ttgaatcgac
     4021 acgggttctg atgacgtgat tttgggtatt aatttgatga acaaccgaac cagatgaggc
     4081 gataacagcg ttactgacgt tgccgttaat ctgattctca acgtttgatt tgccaatagc
     4141 gtcgtccaat tttttattaa ggtcgtctga aacagcattt aaatttgccg aatgaatgcc
     4201 tgtaaagaca aattgaatat ccacccctaa ttggacagca gcaagtaaaa agtccgcagg
     4261 aatgttgacc tgcccgcttt catacagtct caaactttct gcactgcatc cagtttgctc
     4321 agcaaaattc tttcgagagt atccaagtcg gcttctctct ttaactaaat tcaatgctaa
     4381 atcaatgcgt tccataaaac accgcaaaat agtacccaat atatttggta taccaaatat
     4441 attgggtata tcatccaggt aacagttaag gcaggcatta aagcctgtaa ggtaagtaag
     4501 taatggattc tatcatggag gcattatgca agcgaacgaa atctataaat ccttagaaga
     4561 aaaaaatata tcggcaagaa tgttggcttc ggctttaggg gtaactaacc aatctgtttc
     4621 tgaggtcata agaaatggac gaggaagcaa aagaattgcg gaggcaatcg cgaaagtgat
     4681 tcaaaaagat ttagttgaag tttttccgca ttacaagaaa acagattgtc gagacaaaaa
     4741 gatagctgaa ttgaaagaga tgctcggagt gggttgaaat ggaatatatg gatctcaaaa
     4801 gcctgcttaa ttttgggttg ccaaatatgc caagaacagt aatagggctg agaaaaaaaa
     4861 gcgcaaaggg atggttggca aacgagaagg cgtcaaggta agggtggtgg tgttgaatat
     4921 gccctaccta ttgaaatccg agcagccatc atgaaacggc agtcggacga gctggcggaa
     4981 aagatgccga aaatgttgcc ccaagtcaga ccggggacgg cgatgtcggc tcaggcactg
     5041 gctgaagcgg ccaagctgtt gaacgagaaa caacggtcgg tggcggatgc gcgatgtgcg
     5101 gtggtggcgg cggtattggg gattaaatac caatacggtt gctctgcaaa ggctgcggtg
     5161 gctcagtttt tgggcttgct ggcagaaagt aaattggacg cggtcacgct tgggaacttg
     5221 gaaaaggcca atgaccgcag ccggacggca aaggttggcg aacgtacttt agacggctgg
     5281 atttctgctt atttgaaagc ggaaaacgcg acggagcggt tggttgcttt ggctccgaag
     5341 acgacgaagg cggtcaagcc gattgagagc tacggttggt tgccgatgtt tatgcagttt
     5401 cacaatatcc cttcggctcc gaaactggcg cacagctacc gccggtttgt gcagtgggcg
     5461 gaagcggaaa atatgccggt caatgatgtg cctaacttga gtatggtgcg gcgcgtttgg
     5521 gacaagctcc cgctgattat gcaggagcgc ggcaggaaaa cgggggcggc ttataaatcg
     5581 ctgttgcctt atgtgaaacg tgattggggg gctttgaagc cgaacgatgt ttggatcggc
     5641 gacggccata gctttaaggc aaaggtggcg catccggtac acggcaggcc gtttaagccg
     5701 gaagtgacgg tgattattga tggttgtacg cggtttgtgg tcggtttttc ggtgtcgttg
     5761 gctgaaagtt gtgtggcggt atcggacgct ctgcgtatcg gggtcaagca ctttggtttg
     5821 ccgattatct actactcgga taacggcggc ggccaaaccg gcaagacgat agaccatgaa
     5881 atcacgggta ttacgtcccg attgggtatc cgccatgaaa cgggtatcgc gggcaacccg
     5941 caagggcgcg gcatcatcga acgatggtgg aaagacaatc tgattgagat ggcgcgacag
     6001 tatgagacgt ttgcgggcgc ggggatggac agcagcacga agaacctgat gtaccgcaag
     6061 atggaaagtg cttttaatgc tttggaaaaa ggcaaggagt tgacggtaga acagcaaaaa
     6121 tatttgaaaa aactgccgag ctggtcgcgt tttatcgcgg atgtggccaa gtgtatcgac
     6181 gaatacaaca accgcccgca cggcgagctg ccccgacatc ctgacggcgg gcattattcg
     6241 ccgaaggctt atcgggaaat gaggctggaa caggacggta tcgcgccgga tatgttgtcg
     6301 gcggaagagc tggcgacgat gtttatgccg caggaggtgc gaaaagtaca gcgcggttgg
     6361 ctggatttgt tcaacaactc ttatttctca accgagctgg cggagtatca caaggacgag
     6421 gtacgggtca gctacgattt gagcgatgcg tcggcggtca atgtgtttga tatggacggc
     6481 aagtttatca ctaaggcgca ggccaacggc aatacccgcg aggctttccc gacggctcgt
     6541 atcgaccaac tggcggaaaa ccgccgaaaa ggcaaaatca agcgggcgga aaatgcaatc
     6601 aagctcgcaa atgcggaagt caatccggca ctggaacagg ctgcggtttg ggacgagctg
     6661 ggacatttgg gcggaaacgt catcgaggcg gagtatgcgg tattgccgaa aacgggcacg
     6721 gacgacggga ttttcttgtt tgaggcggat atgtaaagga aaacatgatg gaaaaacaga
     6781 aaaatgcggc gttttcggcc gagcttgttg aaaaattgaa actcaagcga gcccttgggc
     6841 ggattcaacg atttcaagca aagattcaag gtgttcccgc tgaacggaat caggctcaaa
     6901 tttctaaatc gggaaactct ttcatttcag acggccttta aaggttgttt aaaactcaag
     6961 gatattaaaa atgaaacaaa ttaatcaagc attgcaacaa aaactggttg aatttaaaga
     7021 aaaatcgggt atgaaccaaa cccaactggc acgcggtatc gatacttcac cggcctccat
     7081 cagtatgtat ctgaacggca cttatgccga taaaggcggc aattatgaaa ccatcgagac
     7141 gaaaatcgag gcgtttttgg agatgcagga cagtaaagcg cgacgtgaag agctggtgtt
     7201 gggatttgta tcgactaaga cgacacgccg tattgcggaa gcgatgcgcg atgcgcacga
     7261 aggcggcgaa atagtggtga tctacggtca ggccggtttg ggcaagactc aggcggtcaa
     7321 aaactactgc gagaaaaacc ctgcggccat cttgattgag gctaatccga gctttacggc
     7381 tttggtcctg atgcgcaagt tggcgacggc ggcgaaggta tcggcgatgg gcagcctgaa
     7441 tgatttgttt gaatcggtat cggacagatt gcgcgattcg ggccgtctga ttgtggtcga
     7501 tgaagcggaa aacctgccgt tacgcgccct tgagattatc cgccgactgc atgatgaaac
     7561 gggctgcggt ttggtgttga gcggtatgcc ccgattggtg gcgaatttgc gcggtaagca
     7621 tggcgagctg gtacagcttt acagccgcgt gtcgcttccg ctgaatttgg gcgaatcttt
     7681 gccggatgaa gaactctttg agattgcgaa agcggctttg cctgatgcgg acgaggagac
     7741 gctcttggaa ctggttaaac atagtaacgg caatacgcgc cggatgagca aattgatgcg
     7801 cggcgcggta cgcacggcga acaagaacgg tatcaagatg caggccggta tcgttaaaaa
     7861 atacagctcc ctgattatcc gatagggccg tctgaaacgg taagtctctg acagggctat
     7921 atattttttt accctatgat tttaataagt tgttgtttta aaaggaaaac gcaaaatgca
     7981 agttttgaag aaagttgatt ggaagatgtt tgtggcacgc tctttttggc ggtgtgtgcc
     8041 ggttggtttg acggcgggcg tgtggtgttt tgtggcgggg atggcgttgc attcctgcgc
     8101 gaaagaacct gaaccggttg cgaaagagct gacgaaggtc gagaagatgg aaagacaggc
     8161 ggatttggag gtcaaggtat gggaagagca atacgagtca atgagcgtcg aggaaaaaat
     8221 gaaagggatt gtttatgagt gataagccaa ttttattgag tcctgcggcg aaaaaagagg
     8281 ctttggatcg ggcggtaaag gaaatccgtg caaaatatgg cgataaggcg attgtgaaag
     8341 gatgtgtgaa atgagtttcg gacgacgtaa tacggattgg caggcttggg gacaacaccg
     8401 caggcgtgcg acggcgcgaa tggcgcaaaa aagccgggag cgcgaaatcg aggaatatca
     8461 ggcgcagttt gaagataagg acggcaaagg tcgtctgaaa acgggaggaa acaaagatga
     8521 atgaaaaaga tttgatcgaa tggctcgaag accgtggcga gctgatggtc atgaagaagg
     8581 acggcgaagg ctttgtgatt gcagcgcgtg cgccggacgg gatgtggaag actgccgagg
     8641 cggaaacttt ggctcaggcg ataactttat gggaggaagt gtgatgttta agtttttgag
     8701 aaaccgcgcg aaagttgaag caattttaaa caagaaagga aagtgaaatg aaagtgtatg
     8761 tttttaaaat tagtaatgaa aacggtaagt taaagataga actccccgaa atcccaatgg
     8821 gtaagcaaat tgacgaagtt gatttgattg ctgggctaac cacggaattt attgcaagta
     8881 tgttacgtga tgcccaaaaa gatcgtcgca aattcgtaat cgacgcatca aatcaattgg
     8941 ctgcaatcca gacatatcaa aaaatcttca attaaggaag gaagagaaaa tggctaaaac
     9001 ccgaatcaaa cagcccgcca tcgaagcggc acaagacaaa acggaagtta ctgcatttat
     9061 ccgccaaatc ggcgacttgc agcgcgaagt caaacgcctg gaaaccgaag ccggagacaa
     9121 aaaagcggct atcgaagaag aatatgccgc caaagccgcg ccgatgtgtg ccgaaatcat
     9181 gagcctgacc gaacgtgtgg ccgcctactg cgaagcgcat aaggacgagc tgacggaaaa
     9241 tggtaaaacc aaaaccgtgg actttactac cggcctgatt aaatggcgca ttcgtccgcc
     9301 atccgtcaag gtaacgggcg tggccgccgt cttggcgtgg ctctcggaaa aatccgcctt
     9361 tgccgagttt gtccgcacta aaaaggaaat cgacaaagac gccatcctga atcaaaaaga
     9421 gcgtttttca gacggccaag tgccggggat taagattgtg agcggcgtgg aggattttgt
     9481 gattgagccg agcgaacaaa ttctataaaa tcaacagcct gatttcaatt cagtgtgttg
     9541 aaacgtgatt gagcctacgg agcaggagct tgcctagccg gttcaaatag gctttaaccc
     9601 atgattaaag gccgtctgaa atggggttta aaacctgttt cagacggcct tttttcatac
     9661 cctagattca ggctgctttc tcttcctgct cgtacacggc gttgtagaaa gcggcggaca
     9721 gcttgtcggc tttctcggag gcggtttcaa tcacggcaga caaaccgtct tcgattcctt
     9781 ccatgtccat atccaaaact ttcagatggt tgagcgtgaa caccagcaga ttcagggctt
     9841 tgaggctgtc ttggtcgaaa gtcagagtat aggtggtatt catggcttaa ccctccaatc
     9901 ccaaagactg ttgttgcacc accattttgg gcttggggac atattccaag aaacccaaat
     9961 cgttgagttt tttgagacgg taggaaaccg cgccggggtt catgtccaag agtttgccta
    10021 tttcggtcag gttcaagccc atactgcggt agcgcagcag cgcgagcatt tcgggcgcgg
    10081 cttggaaata ggcgtcttcc aatgcatcga tgcggtatag cacggcatca ggcagggctt
    10141 ttgcctgttt ctccatttcg ataaaatatc ggcgggcttg gcgtcctttg tcgttgcgct
    10201 ccaccatgca cagctctttt gccatgtcga gggataaata atacccctga acagttttat
    10261 cacgctgtcc aaagaaacct gcttcggtgc ggacaatttt gtccacgccg ataaaatcaa
    10321 gggcttgtgt aaatccgtat tcttcaatgc gacgctgaat ccatttagtt ccacacctaa
    10381 gaacttatgc aggtcatgag catcaaccag tgcttgtgtt tgattgtcca attgacctga
    10441 aacagttggg atgagttggg tattcatggt aaaattatct ttcatttcaa ggttgagatg
    10501 acaaggaaaa gaacgctcta acgttgcttt tccactatcc ccgataagct gtcacttatc
    10561 ggggatttcc atatcttgtg ttgcacaaga tatggaaatt ttaaattatg gtaattctat
    10621 ttgtcaatat tcttttttgc tttctttttg aatttttccg attccatatc tcctctaatt
    10681 ttcgatttta cccagtttga aaaatctaaa ttttcagaaa atttaattaa atcaagttct
    10741 ttttcggaat tgaacgatac tgttttcact actcgcttga gtgcgtattt ttcttgagcc
    10801 tttttaatcg agtccataat cttcctttaa ataggtttta aaacacgatt atggtaatac
    10861 taaaaaccat tttaatcaag atgtattaca acaaaaagcc gtctgaaatg gggtttaaaa
    10921 cctgtttcag acggcctttt ttcatgccct acataaagag agttgctgct ctgctttcca
    10981 cctgttccgc cgatgttttc ccatcggcgg aattttctta tttcattttt tccttcatga
    11041 ctgatttaac ccagtttgaa aagtcgggaa tttctttttg cataaaatcc accaactctt
    11101 tctctctttc caaatggaaa gaaactgttt tcatcagccg cttttggtca tactttcgct
    11161 gatattcagc ttgttttgct tttagcgttt tttcatcagg cataggcaat ccttgatttt
    11221 taaaatcgtt gtagtaaaat ggcttgcaga aaggttggcg aaaggggcgc ggtgttctgc
    11281 caccccttgt gtttaatcaa cttgcttagt aagccggact acagcttaaa agcagaatga
    11341 tgaaaaaaac aactttcgcc agttttgtca tttccacaag cctcctttct atccgataag
    11401 cccccgaagt tgccgcttcg gggctttgct ttatcagata ggcgtattat ctagtatttc
    11461 tagaatttag tcaagctatt ttttgatttt ttctttgatg attgatttaa cccattggga
    11521 aaaatcgata ttttgggcat attccaaaag ttctttttcc gtttcggcat tgaacgagac
    11581 tttcttaatc acccgtttct gctcgtaacg ctttcggttt tctgccagtt tttcatcaac
    11641 cataggcaaa ctccttgatt ttttaaaccg tattttgtaa gattgggact ggggacagcg
    11701 gcgaccgccc ccagctggtc agtctttagt aggcattagc acttgccaac atgaaaactg
    11761 cgaccaagat gagaatttga atagtgcgtt tattcatctt ctttctccca gttgagcccc
    11821 gcttcggtgg ggcttttccc gtatcggccc tcactacgcc gatgaattga attataaacg
    11881 tggacacgtt aaaagtcaac cctttcccca acaaaatcaa agaaaaatgc cgtctgaaaa
    11941 cagctcgaat gctgtttcag acggcatttt ttgttgtcgg gcatcaatgc cctgccattt
    12001 gtgcggcgtt gcggttatca agatatggta ttttagtgtg tatttttaga agaaatttac
    12061 catatatagt attaaaatat tatacggttg ttttatatgg atttgatttt cttgaaaaat
    12121 aaatcccata tgctaatatt tgcactattt gggcgaggtt gaaaaatgaa taccgtcaaa
    12181 gaatatacgg cagtccgcga acggctactc aatgctgccg attatttgga agaagtccgt
    12241 aaagaccgta agacgggcaa tattgcgtcc gtggaatttg tgccgccgaa aataggggct
    12301 cggggttacg gcaaattcaa agtccgttac aaaaccttag tggcggttga tttatgagcg
    12361 gggaaaaatc cgagaatctg ccggccgatg agcagcagaa aatgcttcag gagtttttag
    12421 aacttcaaaa acaggatatt gcagtcaaac agaaggagtt ggaaagccgt aaggacgaaa
    12481 tccaatctca cgaacgcatt gcactggcta ccatcgaagc gcaaaaacag ggtgaacagc
    12541 aacatggtga gattttcaaa gaggtacaca aacgccgtct gaatgccgtg attgctattt
    12601 cttttctgat tgccgccgtg ctgattactg cgttatggcg cgataaggac agcgtcgcta
    12661 ttgaaattgt caaaatcggc ggggcggtag ctttgggata ttttgcagga atcaacagag
    12721 gtaaagcgca aaccttggaa aagcaacggc gcgaaaaaga cagtgattag attgtagtaa
    12781 aaaaggtcgt ctgaaacgtt ttggcgaaac tcgcaaagct cgttttcaga cggc
//
LOCUS       DAWFLT010000058        11969 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWFLT010000058 DAWFLT010000000
VERSION     DAWFLT010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11969
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.058"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            141..575
                     /locus_tag="WGR74_001534"
     CDS             141..575
                     /locus_tag="WGR74_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ0905923.1"
                     /translation="MNFALSVVMLTLASFLPVPPSGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNDVNKYCR"
     gene            complement(624..1967)
                     /locus_tag="WGR74_001535"
     CDS             complement(624..1967)
                     /locus_tag="WGR74_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ0905924.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1972..2625)
                     /locus_tag="WGR74_001536"
     CDS             complement(1972..2625)
                     /locus_tag="WGR74_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ0905925.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2776..3066
                     /gene="gatC"
                     /locus_tag="WGR74_001537"
     CDS             2776..3066
                     /gene="gatC"
                     /locus_tag="WGR74_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ0905926.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAGAPEVRNRLYIVPQVIEE"
     gene            3129..4574
                     /gene="gatA"
                     /locus_tag="WGR74_001538"
     CDS             3129..4574
                     /gene="gatA"
                     /locus_tag="WGR74_001538"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ0905927.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGVKIGLPKEYFGEGNSADVQTA
                     LQNTINLLKTQGAELVEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4571..5503
                     /locus_tag="WGR74_001539"
     CDS             4571..5503
                     /locus_tag="WGR74_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEZ0905928.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLTDIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5546..6976
                     /gene="gatB"
                     /locus_tag="WGR74_001540"
     CDS             5546..6976
                     /gene="gatB"
                     /locus_tag="WGR74_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ0905929.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLNICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAHDYRYFPDPD
                     LLPVIISDDRLQKAKAEMPELPKEMAARFVADYGVSDYDARLLTASRVQAAYFEEAAK
                     ESGQGKLTANWMNGELAATLNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEASIAEIIEKHGLQQMTDTGAIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7068..8078
                     /locus_tag="WGR74_001541"
     CDS             7068..8078
                     /locus_tag="WGR74_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ0905930.1"
                     /translation="MNHTITLPDQTTFAAGDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMGGHSEQALSEAEKAQGKILMCCTTAQSDISINIPGYKADALPVRTLP
                     ARIENIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANPPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQDSGRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPRECW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8208..8840)
                     /gene="pdxH"
                     /locus_tag="WGR74_001542"
     CDS             complement(8208..8840)
                     /gene="pdxH"
                     /locus_tag="WGR74_001542"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ0905931.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            9061..10110
                     /locus_tag="WGR74_001543"
     CDS             9061..10110
                     /locus_tag="WGR74_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0905932.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETDKTASQPY
                     GQKASDGIKPQNLPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPNDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANILK
                     WAEMLLPGEYRRKKA"
     gene            complement(10179..11720)
                     /locus_tag="WGR74_001544"
     CDS             complement(10179..11720)
                     /locus_tag="WGR74_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ0905933.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNTFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSIGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMIFVKA
                     LDLFS"
BASE COUNT         3073 a         3622 c         2970 g         2304 t
ORIGIN      
        1 tttgggaatc tgttttaccc caatatataa agcaccatat taaggcggag tgtcttcccc
       61 actttgaccc gaacccggaa aagacaccgc ccaagccaat cctgatgctg ccccgacggc
      121 caaccattaa ggaaatccta atgaactttg ctttatccgt cgttatgttg accctcgcct
      181 ctttcctgcc cgtcccgcct tccggagccg ccgtctttac ttggaaggac ggcggcggca
      241 acagctattc ggatgtaccg aaacagcttc atcccgacca aagccaaatc ttaaacctgc
      301 ggacgcgcca aaccaaaccg gcggtcaaac ccgcccaagc cgacgcaggg aagcgcacag
      361 acggcgcggc acaggaaaac aatcccgaca ctgccgagaa aaaccggcag cttgaggaag
      421 aaaagaaaag aattgccgaa accgaacggc agaacaaaga agaaaactgc cggatttcaa
      481 aaatgaacct gaaggcggtg ggaaactcaa atgcgaaaaa caaggatgat ttgattcgga
      541 aatacaataa cgatgtgaac aaatactgcc gttgagcagc ctcatcgaaa accccatgcc
      601 gtctgaaacg gcacggggtt tgtttatttc tgccagtagg ttttgacgtt gacgaactcg
      661 tacagcccga attcggacaa ttcgcgcccg taaccggaat ctttgactcc gccgaaaggc
      721 aggcgcaaat cgctgctggt atggcggttg ataaacaccg atcccgcctg tattttttcg
      781 gcaaaccgcc aagcgcgttc ggtatcggcg gtataaatgc aggcacctag cccgaacggg
      841 gaatcattgg caagggcgat ggcatgttct tcgttttcgg cgcgcaaaat cagggcggcc
      901 ggcccgaata cttcttctct ccagacgcgg caggcaggat ttaccctgtc taaaaccgtc
      961 gcgggataaa accagcctcg cccttgtggg atttttccgc cggtcaggca taccgcaccg
     1021 tttgaaacgg catcttcaac ctgcccgtga accctgtccc gcaaatcttc gcggtgcagc
     1081 ggtgcaagcg tagtatcggg atgtttgggg tcgcccattt tcaatttggc gcattcggca
     1141 agaaacagcg tgataaaacg atcggctgcg gcttcggtta cgatgatgcg tttggcggcg
     1201 ttgcacgatt gccccgcatc gcggaaacgg gaataacagg cttctgcggc ggcgcgttcc
     1261 aaatcggcat cgggcatcac gataaaggcg ttgctgccgc cgagttccaa gacggttttc
     1321 ttgaggtttg cgcccgcgtg tgccgcaagg atgcgccccg tatgcgtcga gccggtaaac
     1381 gccattgcat cggtatcttc aaccgccttg agcgtgcccg cctcatccag ccacacgcct
     1441 gccaaaggaa tgccgtctga agccaaatcg aacagtgcct gactgacgcg tgccacgctg
     1501 ggcgcgggtt tgacggcgca cgcgttgccc gcgcacatag cgggaacggc gaaacgcaat
     1561 acctgccaga cgggatagtt ccaaggcatg acggcaaaca ccacgcccaa aggctcgaag
     1621 cgcacctgac tcaaactcgc ctgcgtcgcg atggttttgt gggcaagcag ttcgggggca
     1681 aggcgggcgt aatagcgtat cagttcgata gacttgccga tttccgcacg gcattcgtgc
     1741 aagcagcgtc cgacttcctc acacaccatt tccgcaaaac gctctttctc cgcctccaaa
     1801 cggtcggcaa atttttgcag gcgcgcggca cgttcggtta cgcccagttg cgcgaacgcc
     1861 ccgccgcgca ttttcaaatc cgccagccgc cgttcaaact ccgcataatc ttgagctgga
     1921 cggcggtaaa gcgtttcgcc cgtaaataca ttgacactgt gaaacatcga atcaacctgc
     1981 cagttgcggg aatatcgttt tcagtcccga cacaataatc tccaccgata ccgccgccag
     2041 catcataccc ataatgcggt ttaaaatcgt cagccccgtc gcgcccagca ggcggctgac
     2101 cttcccggca acgattaaaa tggcataaca aatcgcactg accaccaaac cggccgcgat
     2161 aatcaacgcg atgtcgccgt atgttttagc cgccgaagcg taaataatca cggtcgaaat
     2221 accgcccggg ccgatggtga tcggtatggc gatgggcacg acggcaatcg ctccggcatt
     2281 gcgggcgggg cgcgcctgcc ccgtttccgg ctgcgcgccg agattctgct tggcgggatt
     2341 gtcgttgccg ttcatcatag aaatggcaat cagcaacacc aaaattccgc cgccgacctg
     2401 aaacgaaccg acgctgatgc ccaaaacctt cagcagcgta ccgccgatca gcgcaaatac
     2461 cgcaatcacg gcaaacacgg caacggcggc cgtccgcgcg accttcctgc gctccttcgt
     2521 gctgtgcccg ttggtcaggt caaggtaaag cgacaacgcg ctaaacggat taatcagcac
     2581 caaaaaagcc acaatcagct tgccgatttc catgcccaat cccattattt ccccctcctt
     2641 caaacccgtg cggcaggcat ccgatgctgc aaattgccgc cgcaacggat ttttccgtta
     2701 taattaaaaa ttcaagcaat acgccccatc atacccgaac gacggtatct ttaccatcag
     2761 acaaggatgc ttttcatggc actgacactt gccgacgtag acaaaatcgc ccgactctcc
     2821 cgactgcacc tgactgcgga agaaaaagaa aaatcgcttc aagaattaaa cgacattttc
     2881 actatggtcg aacagatgca aaccatcaac acagacggca tcgaaccgat ggcgcacccg
     2941 cacgaggccg ccctgcgcct gcgcgaagac gaagtaaccg aaaccgaccg cgccgccgaa
     3001 tatcaggcgg gtgctccgga agtacgcaac cgtctgtaca tcgtaccgca agttatcgaa
     3061 gaataatccg aatatgcttc agacggcatc agcaataccg cccgaagccc tttaaggatg
     3121 gaagatttat gacccaatac acattgaaac aggcaagcgt cctgttgcag tccaaacaga
     3181 tttccgccgt cgaactggca agcgcatacc ttgccgccat cgccgaaaaa aatcccgccc
     3241 tcaacggcta tatcaccatc gaccaagata aaacccttgc agaagcccgt gccgccgacg
     3301 aacgtatcgc gcagggcaac gcctccgcgc ttaccggcgt acccgtcgcc tacaaggata
     3361 ttttctgcca aaccggctgg cgcagcgcgt gcgcttccaa aatgctcgac aacttcatct
     3421 ccccctacac cgccaccgtc gtccaaaacc tgctcgacga aggtatggta acgctcggcc
     3481 gcaccaatat ggatgagttc gctatgggtt cgaccaatga aaactcattc tacggtgcag
     3541 ccaaaaaccc atggaatctt gagcacgtcc ccggcggttc gtcaggcggt tccgccgccg
     3601 tcgttgccgc gcgcctcgcc cctgccgcgc tcggttcgga caccggcggc tctatccgcc
     3661 agcctgcatc gcactgcggc attaccggca tcaaacccac atacggcacg gtttcccgct
     3721 tcggtatggt cgcctacgcc tccagcttcg accaggccgg tccgatggcg caaaccgccg
     3781 aagactgcgc gattctgttg aacgcgatgg caggtttcga ccccaaagac tccaccagcc
     3841 tcgaacgcga aaaagaagac tacacccgcg atttgaacca accgctcaaa ggcgtgaaaa
     3901 tcggcctgcc caaagaatat ttcggcgaag gcaacagcgc cgatgttcag acggcattgc
     3961 aaaatactat caacctcctc aaaacccaag gcgcggaatt ggtcgaagtt tccctgccgc
     4021 aaaccaagct gtccatcccc gcctactacg tcctcgcctc cgcagaagcc agtaccaacc
     4081 tttcacgtta cgacggcgta cgttacggac accgtgccgc ccaattcggc gatttggaag
     4141 aaatgtacgg caaaacccgc gccgaaggtt tcggcagcga agttaaacgc cgcatcatga
     4201 tcggcactta tgtactgtca cacggctact acgatgccta ctatctcaaa gcccaaaaac
     4261 tgcgccgcct cgttgccgat gattttcaga cggcatttgc acggtgcgac ctcatcctcg
     4321 cgccgaccgc acccactgca gccccaaaaa tcggagcgga tgcttcgccg gttgaaacct
     4381 acttgagcga tatctacacc atcgccgtca acctcgccgg actgcccgca ttgaccctgc
     4441 ccgcaggctt cagcggcggc ggactgcccg tcggcgttca gcttgtcggc aactacttcg
     4501 ccgaagccaa aatcctcggt gcggcgcatc aaatccaact caacagcgat tggcacggca
     4561 aacgacccga atgaagcaga accgcacctt taccttcccc gattttcgca ccgtttacag
     4621 ctatgcgcct ttatatcggc tgcaacattt aaaatacaca ttgcgaaaat ttttcggaaa
     4681 aaaagaaatt tacgccttcg agcagtttgt caacgcatcc cctatccgtc aggggctgtt
     4741 cctccactgc ccgcaaaatg cctatccgct gctgcgcgaa tttgttgaca ggcgttttaa
     4801 ctgcaaacgc cgtttagatg cgatgacggc agattttctt atggcggaaa aactgttcgg
     4861 cacagacatt ctgcaccaaa tggaagacta ccgcttccat ttggtcttgg cgcacctttc
     4921 agacggcatc agcttgtggc tcaaccgcaa cgacaactgc gtcgaagaag gcgcgtggtc
     4981 tttatctttg cgcgacgaag caggcaaccg gctgtatatg gcgaccttcg cctttgtcgg
     5041 cacacacctg ctgacagcct ccgtacaagg gccggcgggt gaagaagcca aagacaccgt
     5101 ccgccgcata accaaacaac tccacggctt gcgtccccaa caactgatgg taaccgccct
     5161 gcaatatttc gccgccgtac tcggcttgga cggcgcaatg ggcattgcac aaaaacatca
     5221 ggtcaaactg cgctggaaac ttaaaaagcg tgtcaaaatg aattacgacg cattctggca
     5281 ggaatacggc gcaagtttgg aacgggacgg ctactggcat ctcccccaaa cccccgcccg
     5341 caaagacctt accgacatcg aaagcaaaaa acgctcgatg taccgcaagc gttatgaaat
     5401 gctggacaac atggttgcag agatgaagga cggtctgaaa acagaagcac gcggcatttc
     5461 agacggcatc caaacggaaa aaccgctccg ccggacagcc tgacgcgaag actatcgaat
     5521 tgatatttta gagaaagaag ctcttatgac ctgggaaacc gtaatcggct tggaaatcca
     5581 cgtccaattg aacaccaaat ccaaaatctt cagcggcgca tcgaccgcat tcggcgcaga
     5641 acccaacgcg cacgccggcg tggtggaatg tgctttgccg ggcgtactgc cggtaatgaa
     5701 ccgagaagtc gttgaaaaag ccatcaaatt gggtttggct ttggatgcga aaatcaaccg
     5761 gaaaaacgtg ttcgaccgta aaaactactt ctatcccgac ttaccaaaag gctatcaaat
     5821 cagccagttg gacttaccga ttgtcgaaca cggcaaattg gaaatcgtag tcggcgacga
     5881 tgtgaaaacc atcaacgtaa cccgcgccca tatggaagaa gacgcgggca agtccgtaca
     5941 tgaaggcttg aacggcgcga ccggtatcga tttgaaccgc gccggcacgc ctttattgga
     6001 agtcgtatcc gaacccgaaa tgcgctccgc cgccgaagcc gttgcctacg ccaaagcctt
     6061 gcacagcttg gtaacctggc tgaacatttg cgacggcaat atggcggaag gttcgttccg
     6121 cgtcgatgcc aacgtatccg ttcgcccgaa aggtcaagcg gaattcggca cgcgccgcga
     6181 gattaaaaac ctcaattcct tccgtttctt ggagcaggcg attaattacg aagcagaagc
     6241 gcaaatcgag attttggaag acggcggcaa agtacagcag gcaacgatgc tgtttgatcc
     6301 cgaaaaaggc gaaacccgcg taatgcgcct gaaagaagac gcgcacgact accgctactt
     6361 ccctgacccc gatttgctgc ccgtcatcat ttcagacgac cggttgcaaa aagcaaaagc
     6421 agaaatgccc gagctgccaa aagaaatggc ggcgcgtttc gtggcggatt atggcgtgag
     6481 cgactacgac gcgcgcctgc tcactgccag ccgcgtgcag gctgcctatt ttgaagaagc
     6541 cgctaaagag agcggacaag gcaagctgac tgccaactgg atgaacggcg aacttgccgc
     6601 cacgctgaac aaagaaggca tggaacttgc cgacagcccg attaccgccc cacgcctcgc
     6661 cgctctggta ggcaaaatcg ccgacggcac attaagcagc aaattggcga aaaaagcctt
     6721 tgaagccatg tgggctgaac ccgaagcctc cattgccgaa atcattgaaa aacacggttt
     6781 gcaacagatg accgataccg gcgcgattga agcgatggta gacgaagtgc tggcaaacaa
     6841 tgcaaaagcc gtggaacagt tcaaatccgg caacgaaaaa gccctgaatg cgattgtggg
     6901 gcaagtgatg aaggccagca aaggcaaagc caatcccgcg caggttcaag agctgattaa
     6961 agccaaactt gcctgataag caagatgccg tctgaaaccc aaatccgacg ttcagacggc
     7021 attttcacag tctgaccaca ttaaaacagc ataaaaaaca atattccatg aaccacacca
     7081 ttacactgcc cgaccaaacc acctttgccg ccggcgacgg tgaaaccgtt ttagccgccg
     7141 ccgcccgtca aaacctcaat ctgccccatt cctgcaaaag cggtgtctgc ggacaatgca
     7201 aagccgaact ggtcagcggc gatattcaaa tgggcggaca ctcggaacag gctttatccg
     7261 aagcagaaaa agcgcaaggc aagattttga tgtgctgcac cactgcgcaa agcgatatca
     7321 gcatcaacat ccccggctac aaagccgatg ccctacccgt ccgcactctg cccgcacgca
     7381 tcgaaaatat tattttcaaa cacgatgtcg ccctcctgaa acttgccctg cccaaagccc
     7441 cgccgtttgc cttctacgct gggcaataca ttgatttact gctgccaggc aacgtcagcc
     7501 gcagctactc catcgccaat ccgcccgacc aagaaggcat tttggaactg cacatccgca
     7561 ggcgtgaaaa cggtgtctgc tcggaaatga ttttcggcag cgaacccaaa gtcaaagaaa
     7621 aaggcattgt ccgcgttaaa ggcccgctcg gttcatttac cctgcaggaa gacagcggca
     7681 aacccgtcat cctgctggca accggcacag gctacgcccc catccgcagc atcctgctcg
     7741 accttatccg ccaagacagc ggccgcgccg tccatttcta ctggggcgcg cgtcatcaag
     7801 atgatttgta tgccctcgaa gaagcacaag ggttggcatg ccgtctgaaa aacgcctgct
     7861 tcacccccgt attgtcccgc cccagagagt gctggcaagg aagaaaaggc cacgtacaag
     7921 acatcgcggc acaagaccac cccgacctgt cggaatacga agtatttgcc tgcggctctc
     7981 cggctatgac cgaacaggca aaaaatctgt ttgtgcaaca gcataagctg ccggaaaact
     8041 tgtttttctc cgacgcattc acgccgtccg catcataatt ccccggtata aaaaagattc
     8101 aggctttccg ttcggaacac aaaaaacttc ccgtccgtat tttccccgta aaaaaatgcc
     8161 gtctgaaacc cgattcctgt tttcagacgg catatgtttt ttcctgttca aggcgacagc
     8221 cgctcgcgta tccagccgcc atccagcaaa cggtattgga tgcggtcgtg cagcctgctc
     8281 ggcctgccct gccaaaactc aatcaaatcg ggaatcacaa tatagccgcc ccaatgcggc
     8341 ggacgcggca cgtgcaaagg atgtttgagt ccgaccgccg ccgcctttgc caccaatacc
     8401 gccttgttcg gaatcacttc gctctgcgcg cttgcccacg cacccaaacg gctctgatac
     8461 gggcggctct caaaatattc gtccgacaat ttctccgcca gcctttcaac acgcccttcc
     8521 acgcgcacct gacgctccag ctccggccaa aaaaacgtca tcgccgcaaa tggatgagca
     8581 tccagcgaac gccccttgcg gctgtgataa ttcgtaaaga aaacaaaacc ttcagaatta
     8641 acttccttca gcagcaccat acggctgttg ggtctgccgc gtccgtcgac cgccgccaca
     8701 ttgaccgccg tcggctcgtt aacctgtgcg cgtaccgcct cgtccaacca ccgctcgaac
     8761 tgctcgatcg gattgtcggc gcaatcgcct tccgacaatt cccgtttgct gtaatcttcc
     8821 cgaatattgt gcaaatccat ttaccgctcc tcttgttatt tgaaagattc tacccccgca
     8881 cgcaaaccga tttcaaccgt tgcacaaact ttgtccccaa agccgcagcg acgatttcat
     8941 ccgcaaaacc gccgcatcag gtacaatatc gaaccgtccg actgaggacg gcattttatc
     9001 aacccgtcct gccgcatacg ccgcagaaga accgctttat caagcgagtt aggaaaaatg
     9061 atgtccaaac agcccaccag caaacgccaa tggcgcgacg gcgcagcccc gtctgccaag
     9121 aaaaccgcca aaccgttcaa aagcaaagcc cgtcccaaag acgaaacgga caaaaccgca
     9181 tcccaacctt acggacaaaa agcttcagac ggcatcaaac ctcaaaacct ccccaaacag
     9241 cgcgccgcca aagccaaaaa actcgtcgtc cgcaatccca accaaaaaat tatggaacac
     9301 gcgcgcgatt taaaagaacg ccgcagcgac ctatcgcgca tggaacccga acgcctgcaa
     9361 aaagtgcttg ccgcgtccgg cgtcggctcg cgccgcgaaa tggaagaatg gatcaacaac
     9421 ggctgggtaa cggtcaacgg caaaaccgct caactgggcg acaaagtcac ccccaacgac
     9481 cacgttaccg tcaaaggcag catcatcaaa ctcaaatggg ccgaccgcct gccgcgcatc
     9541 atcctatatt acaaacaaga aggcgaaatc gtttcccgcg acgacccgca aggccgcgtc
     9601 agcatattcg accgcctgcc gcaggccgcc agcagccgct gggtcgccat cggacgcttg
     9661 gacatcaaca ccagcggact tctgattctc accacctccg gcgaactcgt ccaacgtttc
     9721 gcccacccca gcttcgaagt cgaacgcgaa tacgccgtgc gcgtactggg cgggctgacc
     9781 ggcgaacaaa tgcgcgtcct caccgaagaa ggcgtgatgc tcgaagacgg cttggcaaaa
     9841 gtcgaacgca tccgcgaaca aggcggcgaa ggcgcgaaca aatggtacaa cgtcgtgatt
     9901 aaagaaggcc gcaaccgcga agtgcgccgc atttttgaaa gccaaggact caccgtcagc
     9961 cgcctcgtgc gcgtcggctt cggccccatc ggactgccca accgcctcaa acgcgggcag
    10021 ttctacgaac tcaaccccgc cgaagtcgcc aacatcctca aatgggcgga aatgctgctg
    10081 ccgggcgaat accgccgcaa aaaagcctaa acccgccaaa acacaaaaat gccgtctgaa
    10141 acatctgctg tttcagacgg cattttgttc gggcgttttc aggagaaaag gtcgagtgct
    10201 ttgacaaaga tcatcaccac gccgtaggcg agcggtatga cggccaatgc ccaacgccac
    10261 cagaccgata tgccgccgcc ggaaactttt tcgcccacaa ggtaagcgtc ggatatggcg
    10321 gtttcgtcat cggggttgcc gctgtgtgcg gcggttttga tgtctttttc gtggtgtttt
    10381 tcgtgtacgg atttgacggc gaggttgcac aacaaaccga taatcagcag acccgccatg
    10441 atgtacatgg ttacgctgta tgcctgtgcc gccggtatgc cgctgtcgat ttggctttgg
    10501 cgtatgtaat tgaccagtac cgggccgatg acggcggcgg ttgaccaggc cagcaggatg
    10561 cgtccgtgaa tcgcgccgac ctgataggtg ccgaacaggt ctttcaggta ggcgggaatg
    10621 gcggcaaatc cgccgccgta catggaaata atcacgcaaa agccgatgat gaacagggct
    10681 ttgctgccgc cctcgccgat ggagggaacg gcgaaataca gcagcgaacc gagtacgaag
    10741 aagatggtgt aggtgttttt gcgtccgatt ttgtcggaaa cgctcgacca caaaaagcgt
    10801 ccgcccatgt taaacaggct caggaggctg acgaagcctg ccgccgcacc tgcgccgact
    10861 gccgcctgcc tgcctatgga ggtttcggaa aagagttcct gaatcatcac ggatgcctga
    10921 cccaatacgc cgatgccggc agttacgttc aggcacaata cccagaacaa cagccaaaac
    10981 tgcggcgttt tcatggcttg ggacacgttg acatgattgc tgctgaccag cttgttttgc
    11041 gttttcggcg cggtatagcc ttcaggtttc cagccgtcgg caggtacgcg gatggtaaac
    11101 gcgccgaaca tcatcagtgc gaggtaaagc agacccaata cggcgaaggt ttcggcaacc
    11161 ccaaccgaag cagcgtttga aaaggtgttc atcagtgata cggaaagcgg cgaggccagc
    11221 attgcgccgc caccgaaacc cataatcgcc aaaccggtcg ccatacccgg cttgtcggga
    11281 aaccatttca tcagtgtgga aaccggcccg atgtagccca aacccaagcc tacgccgccg
    11341 atgacgccgt tgcccaaata gagcaggaag aggttgtgcg tacgcacgcc gaatgcggat
    11401 acgaagaagc ccaggctgaa acagcaggcg gcggcaaata tggctttgcg cggccctacc
    11461 cgttccatcc acgtaccgaa cagggcggcc gacgcgccca gcatcgcgag tgcgatactg
    11521 aaaatccaac ctacggtcgt cagcttccaa tctccggccg ccgattcggt tatgccgata
    11581 agtttggtca gcggcgcgtt gaatacggaa taggcgtaaa tctgcccgat ggcaaggtgt
    11641 accgccaatg ctgcgggcgg tacgagccaa cggttgaaac ccggcttggc aatgcttgcc
    11701 tcacggtcta aaaacttcat aacatcctct ttctgtcagt tgaaaaataa aatttcattt
    11761 gctcaatgga aacttattga aaattataaa aaaatatcgg gtcgggtttt tatccgcccc
    11821 aagatgcgcc gtctgaaaca tttcgggtgt acggaaaggt ttctgttttt tccgacaaat
    11881 tcctgcggct tttcgcttcc ggattcccgc ttttgcggga atgacgaatt aaagattatc
    11941 ttaaggtcaa gggactggat tcccgcttt
//
LOCUS       DAWFLT010000059        11961 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWFLT010000059 DAWFLT010000000
VERSION     DAWFLT010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11961
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.059"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(224..1561)
                     /gene="ribB"
                     /locus_tag="WGR74_001545"
     CDS             complement(224..1561)
                     /gene="ribB"
                     /locus_tag="WGR74_001545"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ0905934.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPASVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERIQQAESGVVILLHRAEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILVGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(1783..2796)
                     /locus_tag="WGR74_001546"
     CDS             complement(1783..2796)
                     /locus_tag="WGR74_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0905935.1"
                     /translation="MIFSIIVPIYNVEKYLRCCVDSVLAENFADYEMILVDDGSPDGC
                     GKICDEYAGKYPHIKVIHQENGGLSDARNAGIRAAKGDYLIFLDSDDYWADTNRSKNA
                     GGGILFDLQQLADKKVDLILHPSSFNYRDIPKGADFSDNDFVRHFETLVEGRYYIANA
                     WTKIVRREIIIKNNLFFPKGYIHEDFPYSLQLARFIKTFAFYDNPFYQYRVLGGSISH
                     NIKYKNFSDVLTHLDRGVDFLVENKNSPIYGGLQKFVFDNIGYLRSILVRLYFSKNII
                     LIYRKYFSFKEKCRKIFGAKTIRPVFIGKTAFIIGLPILRLLVPPMLYPAIKAVYQKF
                     FSE"
     gene            complement(2952..3545)
                     /gene="ribA"
                     /locus_tag="WGR74_001547"
     CDS             complement(2952..3545)
                     /gene="ribA"
                     /locus_tag="WGR74_001547"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ0905936.1"
                     /translation="MSELLNYVASCRLPTEWGVFTMHGFEEANGQEHVALTVGDFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(3538..4212)
                     /locus_tag="WGR74_001548"
     CDS             complement(3538..4212)
                     /locus_tag="WGR74_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905937.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRTAYLTAQTVMDMGTTSIERSV
                     LWLEHEDWRLADYLEQNSGNEEGLKRLFMALDSVFSRCKGVRAAAVYALMPSENAAFR
                     LVCLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            4366..4450
                     /locus_tag="WGR74_001549"
     tRNA            4366..4450
                     /locus_tag="WGR74_001549"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4400..4402,aa:Leu,seq:tag)
     gene            4971..6005
                     /gene="purM"
                     /locus_tag="WGR74_001550"
     CDS             4971..6005
                     /gene="purM"
                     /locus_tag="WGR74_001550"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ0905938.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
     gene            complement(6479..7135)
                     /locus_tag="WGR74_001551"
     CDS             complement(6479..7135)
                     /locus_tag="WGR74_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0905939.1"
                     /translation="MTIKIILIDDHTLFRSGIKSLLSRQHDFEVIGEAADGLSGIKMI
                     NRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDNDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGRSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
     gene            complement(7132..8904)
                     /locus_tag="WGR74_001552"
     CDS             complement(7132..8904)
                     /locus_tag="WGR74_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ0905940.1"
                     /translation="MILPARFSDGISLSLRLKLLTGVWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRSQIDNQVDEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGESDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGTKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIRAV
                     LKIYSQPQQGTTVSLAGVSEESLK"
     gene            9110..9724
                     /locus_tag="WGR74_001553"
     CDS             9110..9724
                     /locus_tag="WGR74_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEZ0905941.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPDDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQTDLQEG"
     gene            9887..10504
                     /locus_tag="WGR74_001554"
     CDS             9887..10504
                     /locus_tag="WGR74_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ0905942.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGYNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            10512..11375
                     /locus_tag="WGR74_001555"
     CDS             10512..11375
                     /locus_tag="WGR74_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0905943.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLARLAGVGRQTELLVRNTEQFIAGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(11388..11822)
                     /locus_tag="WGR74_001556"
     CDS             complement(11388..11822)
                     /locus_tag="WGR74_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ0905944.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPLNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
BASE COUNT         2891 a         2861 c         3255 g         2954 t
ORIGIN      
        1 tgccgttctt tccctgaaag agagaatcca aaaaaccaaa gccacaggaa tttatcggaa
       61 atgacaaaaa cccgacgaac cggattcccg cctgcgcggg aatgacgaat tagaagttac
      121 ccgaaatttg caaaaacaaa atccaaccca acagaccggg ttttcgtttg cacggaaata
      181 atgcaataaa taaagcaaat ataaagtatt tgaatttact atattatttt tccgcttctt
      241 caaagccgac gacttccaaa ccgaagccgg tcaggccggt gaaagatgag ggctgcccga
      301 ggacgcgcag ttttttgacg ttgaggccga cgaggatttg tgcgccgatg ccgtagcttt
      361 tgctgtccca tttgtaggct tggtttgcac ctttgggcag ggtgcggtcg agcagggatg
      421 cgccgtcttc ggcgcggtgt aagaggatga cgacgccgct ttcggcttgt tggatgcgct
      481 caagggcttt gggcagcgac catgaatgac gcgggttagc ttggatgaag tccatgacgc
      541 tgaagggttc gtggacgcgg acgagggttt cggtgtcggc ggagggcgtg cctttgacga
      601 gggcgaggtg ggtttcgccg gagagtttgt cgacgtaaac gtgttgttgg aactcgcccc
      661 acggggtttg tacaggcgca ttgcccatgt cttcaagcag gctttcggta cggctgcggt
      721 attcgatgag gtcggcaatc gtgccgattt tgagcttgtg ttcttcggcg aatttcatca
      781 gttcgggcat acgcgccatc gtgccgtcgt cgttgatgat ttcgcaaata acggaggcag
      841 gaatcagccc gttcatttgc gccaggtcga cgccggcttc ggtgtgtccg gcgcggacga
      901 gtacgccgcc tttttgggcg cgaagcggaa agatatgacc gggttggacg atgtcttcgg
      961 gtttagcggt cggggaaacg gcggtttgaa tagtcagggc gcggtcggcg gcggaaatgc
     1021 cggtggtaat gccgtgtgcg gcttcgatgg agacagtaaa gttggtgccg tattgcgcgc
     1081 cgtttttttg ggtcatcatc ggcagcccga gtttttcgac catttcgccg tccatcggca
     1141 ggcagaccaa gccgcgcgcg tgtttgatca tgaagttgat ggcttcgggc gtgacgaatt
     1201 gcgccgccat cagcaggtcg ccttcgtttt ctcggtcttc ggcatcggtg atgatgacca
     1261 ttttgccggc tttgatgtcg gctaggattt cggggatggg ggaaatatgg gacatggtgt
     1321 gttttcctgt gtgttcgggc gggcgggcgg tgtgttcggt atcgagccag aaggcgggca
     1381 tttttgccgc ctgttcgatt ttacgggctt ttttctcgcc gaaggatttg tttttcaaaa
     1441 gggcggagag ttcgcctgtg ttgagggcaa cggcttcggc aaaacgggtt tgcaaaccgc
     1501 cgtagtgttc tgctatccat tgccgcaggt tgcggcggcg cagaccggct gtgtcggtca
     1561 tcgtttcatc ctttgtagaa atcggatgta gcggattgta atcgcgtatt taccaaaaag
     1621 caaatctgtt tttttcgcca aaccgaatta tttgcttttt ggtaaacaga tgccgtctga
     1681 agccggattc gtggcttcag acggcattgc cgcaatcctt gccggccggg ggctgtttgt
     1741 cccaacttcc cgcgcacttc tgatttgggg tttgccggaa gcttattccg aaaaaaattt
     1801 ctgataaacg gccttgattg ccgggtacag cataggcggt acgagcaggc gcaatatcgg
     1861 caatcctatg atgaatgcgg tcttcccgat aaaaaccgga cggattgtct tcgcgccgaa
     1921 tatctttctg catttttctt taaatgaaaa atatttccgg tagatgagga taatgttttt
     1981 ggaaaaataa agccttacca atatagacct cagatagccg atattgtcga agacaaattt
     2041 ttgcaaaccg ccgtagatgg gggaattttt gttttcgact aaaaaatcca caccccggtc
     2101 gagatgcgtc agcacatcgc tgaaattttt gtatttgatg ttgtggctga tagagccgcc
     2161 gagaacgcgg tactggtaaa aagggttatc gtaaaaggca aaagtcttga taaaacgcgc
     2221 caattgcaaa ctgtacggga aatcctcgtg aatgtatcct tttgggaaaa acagattgtt
     2281 tttgatgatt atttcccgcc tgacgatttt tgtccacgcg ttggcgatat agtaccgccc
     2341 ctccaccagc gtttcaaaat ggcggacaaa atcattatcc gaaaagtccg cccctttggg
     2401 gatgtcgcgg taattgaagg acgagggatg caggatcaaa tcaacctttt tgtctgcaag
     2461 ttgttgtaaa tcaaagagaa tccccccccc cgcgtttttt gaacggttgg tatcggccca
     2521 ataatcgtcg ctgtccaaaa agattaggta atcgcctttt gccgcccgga taccggcgtt
     2581 gcgggcatcc gacagcccgc cgttttcttg atgaatcact tttatatgcg gatatttgcc
     2641 tgcatattcg tcgcaaatct tcccgcagcc gtccggcgaa ccgtcatcga ccaaaatcat
     2701 ttcataatcg gcaaaatttt cggcaagcac ggaatccacg cagcagcgga ggtatttttc
     2761 cacattgtaa atagggacga tgatggagaa aatcataaat atcaatccat tatattaaga
     2821 tgtttgcgcg tatgcctcaa acccgcgctc gcaatgcgtt tgcatccact ataaaactta
     2881 ttgctttatc aaggtatgga aacctgtttc ccgaaaggcg gcgcaggatg cccgttccct
     2941 gcaactttgc cttattccga catcagatgt cccagcttgt ctgctttggt ttggaggtag
     3001 cgtttgtttt caaggttttc cccgacgtgc agggggatgc gttcgaccac gttaatcccc
     3061 gcatctttca gggtttggat tttttcgggg ttgttggtca gcagtttgac cgagcggatg
     3121 cccagatatt cgtagataga ttgcgccaaa cggaaatcgc gggcatcgac gggcagcccg
     3181 agtgccaaat tggcttcaac cgtatccata ccttggtctt gcagatgata ggcgcggatt
     3241 ttgttaatca gcccgatgcc gcgtccttcc tgacgcagat agacgatgat gccgcgcccc
     3301 tctgtctgta cggccctcat ggccgcttca agttgaggtc cgcagtcgca ttttctcgag
     3361 aacagcgcat cgcccgtcag gcattcggag tggatgcgcg tcagaaccgg attgccgtct
     3421 gaaaaatcac cgacggtcag cgcgacgtgt tcctgcccat ttgcctcttc aaagccgtgc
     3481 atcgtaaata cgccccattc ggtcggcagg cggcaggaag caacatagtt taataattca
     3541 gacattttca tctccttctg cggcagtgat gcccaaaagc tgcttcaaag gttcggacaa
     3601 agccaaggca agagccaccc attccacctg tgctgccgta cccgcacact ctgcgcattc
     3661 aaattccaca tgaaccacgc ccaacacacc gccactttcc gtgcagaccg gaatggaaat
     3721 ttgcgccgcc gaagcatgat tgcgttctcc cgaaagctct cccaaatcca accaacggcg
     3781 tacatccgag gcaacattca tccagccgct ttgcgccgaa cggcaagcca gcgaaacctt
     3841 gcctgccgct tcatccaaat cccacaggtt ttccaaaacc tcgccctgcc gggacaggca
     3901 aaccagccga aaggcggcat tttcagacgg catcagggca taaacggctg cggcgcgcac
     3961 gcctttacag cgtgaaaaca ccgaatccaa agccatgaaa agccgtttca gaccttcctc
     4021 attcccgctg ttttgctcca aataatcggc aagcctccaa tcctcatgct caagccacaa
     4081 aaccgaacgc tcaatcgaag tcgtccccat atccattacc gtctgcgccg tcagatacgc
     4141 cgtccgaacc tcgtcaagcg gcagcttcaa accctgagtc agcagaaaat ccttaatcaa
     4201 agcagcaggc atacccaaat tccgtcaata aaaacaaaaa accgcccgat tccgatgtca
     4261 ggcggacgaa aaacagattt taccgcccca aaccccaagc catcaacagc cccgccgaaa
     4321 accttgcaca aaaaaacaaa atctgtataa tcgtccaatt cccatgcgga cgtggcgaaa
     4381 ttggtagacg caccagattt aggttctggc gccgagaggt gtgagagttc gagtctctcc
     4441 gtccgcacca ataatgataa ataaattata tagttaatgc ttatcgggac aaattagtgc
     4501 cacaaaacat aagtcgtctg cttttcagcg ggcggctttt ttatctgcct atatgtttca
     4561 acacgcagaa cggtacataa agtgccgcca tttgttccgc accgaatcgg aaacgggtgc
     4621 ttccgcccgc aatcagattc actgccccta aatgccgggg tttcaaccgg aaagaagaaa
     4681 atattgattt tgatgatggc atcaaaaaac cccttccgcc cgtctgccaa aaggaatggt
     4741 taagaaaagc aatgcgtctt ttgatggaac gcgatatata aggaattgag gggaagggca
     4801 ggcgggtttc tgcccgttaa tcctgcggac ggtcccgcca aacccgacac gcgcgggcat
     4861 atggcggatt gtttacaata ttgtctaaaa atctgaacct gcaaacgtga aataaggtag
     4921 aatacgcccc gttattttac cgtccaatct ccatttacca aggaaaaacg atgagtactt
     4981 cattgagtta ccgcgatgca ggtgtcgata tcgacgcagg cgaccaactg gtcgaaaaca
     5041 tcaaaccgtt tgccaaacgc acgatgcgcc cggaagtatt gggggatttg ggcggcttcg
     5101 gcgcgttggt cgaaatcggc aagaaatata aaaatcccgt attggtcagc ggtacggacg
     5161 gcgtgggtac caagctcaaa cttgcctttg attgggataa acacgatacg gtgggcatcg
     5221 accttgttgc aatgagtgtc aacgatattt tggttcaagg agcagaacct ctgtttttct
     5281 tggattattt cgcctgcggc aaattggatg ttccgcgcgc gaccgatgtg atcaaaggca
     5341 ttgcacaagg ttgcgaagaa tccggctgcg ccctgattgg cggggaaact gccgaaatgc
     5401 cgggtatgta tcccgtcgga gaatacgatt tggcgggttt tgctgtcggc gtggtggaaa
     5461 aagagaatgt cattaccggc cgcagcatcg gcgcgggcga tgtggtattg ggtttggctt
     5521 ccaacggcgc acattcaaac ggctattccc ttatccgtaa aatcatcgaa cgcgacaatc
     5581 ccgatttgga tgccgagttt gataatggca aaaccttgcg tgaggctgtt attgcgccga
     5641 cccgtctgta tgtgaaaccg attcttgccg ctttggaaaa atttaccatt aaaggtatgg
     5701 cacacattac cggcggcggc attaccgaaa acgtgccgcg cgtgttgcct gaaaacacgg
     5761 ttgcacaaat cgatgctaaa tcgtgggaat tgcccaagct cttccaatgg cttcaaaagg
     5821 cgggcaatgt ggaaacccaa gaaatgtacc ggacctttaa ctgcggcatc ggcatggtcg
     5881 ttattgttgc tgccgaagat gccgatgcgg ttcagggtct cttgggtgaa caaggtgaaa
     5941 cagtttaccg tttaggtttg atccgtgagc gtcagggaga cgagcatcaa acccaggttg
     6001 cctgattgct tctatagtga aatgccgtct caaacatggg tttggatggc atttttatag
     6061 tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacagat agtacggcaa
     6121 ggcgaggcaa cgccgtactg gtttttgtta atccactata tattgattga tatgagggtg
     6181 ggttacacat gcttaaacga tataagccat tgtatattta tgggattcgg aatggaacat
     6241 tttaccctgc attagtattt ccgcttttta ttatgccgtc tgaaaatatc atgatgatgc
     6301 gttttcagac ggcatagctg atattgcagt atcagatagg ggcaaacgga aaacactaaa
     6361 ccgaacaata ttactgctat gccaagcgta cttatggggg cataagctta catgcttggt
     6421 ctgttatccg aatgaccgta cggggattaa accccctaat atgccgtctg aacccgcctt
     6481 attccagcac gggttggggg acgttgtgcc ggatggcgta aacagcggcc tgcacccggc
     6541 tgctgaggtt gagtttgcgg agcaggtttt gaacgtggac tttgacggtg gattcggcaa
     6601 gatcgaggtg gcgggcgatg attttgttgc tgcgtcctgc ggcgagatag cccaagattt
     6661 ccagttcgcg aggggtaagt gaggagagtg cctgtgtccc ttgggcaggt tggggggaaa
     6721 tcaggctttt aacgagtttg gcggtcatct cgggcgagaa tacattatcg ccttcggcgg
     6781 ctttgcgtat gctttcgagc agaaagtcgg cgttgatgtt tttcagcagg tagccgcgcg
     6841 cgccgatgcg catacattcg gtcaagtcgt cgttgtcttc ggaaacggtc agcataatca
     6901 ctgcctgctg cggattgatg ctgatgattt gggagagtgc ttcgcgtccg ttcataccgg
     6961 gcatatcaag gtcaagcagg acgacatcgg gttggagccg gttgatcatt ttaatgcccg
     7021 agaggccgtc tgcggcttcg ccgatgacct caaaatcatg ttggcgcgac aaaagggatt
     7081 taatgccgct gcggaagagg gtatggtcgt ctatcaggat aattttaatg gtcatttcaa
     7141 gctttcttca gatacacccg ccaatgagac ggtggttccc tgttggggtt gggaatagat
     7201 tttcaatacg gcgcggatac gtttggcccg ctcctgcatg atgtgcagtc cgacatggct
     7261 gcccgtgggt tctcctattt tctccgtgtc gaaaccttgt ccgttgtctt ggatggtcat
     7321 ggtaaagcgt ccgccgtgtt cggaaagggt gaattttaca tgggtggcgc gggcgtgttt
     7381 gcggatgttg gacaggcttt cctgcaggat aaaaatcatt tggagctgcg cttcctgagg
     7441 cggcaggaac gaaccgtttt cccaggcggt ttcgaccgtt atcccggttt gttgcgtaaa
     7501 gcgggcgaat aggtcggcaa cggcttcggg aaattcttta ttgctgattt tggtacggaa
     7561 gttgagcagc agttcgcgga catcttcata acattcctgc acgcctgttt tgatgaagcc
     7621 gatgttttct gcggcttcct cccgtttgtt ttcggcaaag gcggtttcca gcatctgtac
     7681 ctgtaggttt aggaacgtta atgcttgtgc gatgctgtca tgtaatcctt gcgcaatcag
     7741 gttgcgttcc tgcaatactg caagcaggcg tttttcttcc tcctgttttg tgccggcaag
     7801 cgatacgccc aattgcctgc ctagtgtttg aagcaggatg cggtcgtctt catcaagaga
     7861 aatgccgttt ggaaagctga gcaacagcct gcccaatgtt tcgttctggt actcaatggg
     7921 gaagatttcc tcatggtact tccccaaatc cgaagctgct gtgccgcaat ccgcatgatg
     7981 aatggaaaca taaacatcgg attcgccgtc caaacaaact ctgccggaat ctgctcctac
     8041 ggcgggcagg atacggttta gaaaatgttc tgcagcctgt tgcggtatgt aggattggtg
     8101 caggtcccgt gtagtttggt acagcagggt caggttttga ttttgttttt cgagactgcg
     8161 tgtctgctcg gcgacttgtc cttccaaatc atcatataaa attttcaacc tgccgcccat
     8221 ttgattgaaa caacgcccga cctgtttgaa ttccggcgta ccgccttcgg gaaccggaat
     8281 atcgaaacac ctccgtccga tgcgttccgc accttccctt aacgcctgca gcggccggat
     8341 aacccaaatc tggtgccaaa acagcatcag tacagacgac accagcgtca tcaacataat
     8401 tgcccattga aaacgcctga gccaccatgt gtttttttcg ttggcatttt ccaatgcctg
     8461 caaaaacagt tcgatgtttc cggcaaagcg gtagagatcg acctgagtcg gtcgccggta
     8521 ggactggagc ggggggagga tgtgtgcctg ccaatctata atcagcatgg attgtatcaa
     8581 atcataagca agaggggtgt ccgaaggaat cagcggatgg atggcatcac tttgtgaaat
     8641 acgattcagg cttttttcaa attcgtcaac ctgattgtct atttgggagc ggggagatcc
     8701 ttcacccgcc atgtatgcca gacggtatgc ctgcattctc aagttgcccg cctcttcgat
     8761 gacggaggcc gcgttttcca gacgcaaaga gagcagcagt gtcaaaacga cagacaatgc
     8821 cgccaacccg acccacactc cggtcaggag tttcaggcgt agggaaaggc tgatgccgtc
     8881 tgaaaaacgg gctggcagta tcatgctcgg cgaaaaattg ttccaaataa tgcaaacaat
     8941 atcattcttt ggaattagat acaactgctc agaaagaatt ggttaaggaa aacttaatcc
     9001 gcaccgcttc agtgttaaat catgttatga taaaattcag ataatatgaa tttatttgcc
     9061 gtctgcttca gacggcattt tgagtcattg ggaacggcag actgcaatca tgaaaacctc
     9121 gaacttttcc aagagaacat ccgcaatgaa aacttttgcc ctgatactgg ccgacggtca
     9181 ggcgagccgt atgggaggcg aggacaaggg gcttgctctt ttggggggta aggcactgat
     9241 agatcatgtc atcgacaggg tcaggccgca ggtcagccat atcgccatca gcaccaaccg
     9301 gaatttggaa gaatatgctc gaagaagtcc gcatattttt ccggacgcac ggcagtggca
     9361 gcatttcggc ccgctttcgg cattgtgtac cgcagccaac gatttgcagt tggcgactgc
     9421 cgactggctt ttggttgtgc cgtgcgatat gccgtatctg ccggacgatt tggtggcgag
     9481 gtttgaaacc gtgtcgaaac gcacaccgtt gtgcaatgcg tattatgtgg aaacgccgat
     9541 aacgatgcac tacaacatta tgtatatccg cccgcagatt ctgcaaagcg cgattcccta
     9601 tctgttttcg ggtatgaaaa cattaagaag ctggttgcag cagcaaaggg cgcgaccggt
     9661 cagattcgag tttgacgggc attttgccga cttgaacacg caaactgatt tgcaggaggg
     9721 ataagggtaa gaccgccgac cggcgtggaa aggaaaggtc aagccgtacc gggcggtttt
     9781 tgcccgaatc ggaggcataa tgctgtctga aggcatttca gacggcattt ttcgcgggga
     9841 gatgctacaa tttgcaccat ttttaccgac acagggaaac aggattatgt ttacaggcat
     9901 tgttcaagga ttgggaaaac tgacggcaat ccaccgcccg tcggaggcat ttcagactta
     9961 tgtcgtcgag cttccgcaag aggcggcgga caatctgcaa cgcggcgcat cggtcgccaa
    10021 caacggctgc tgcctgacga ttaccgaaat cgaaggaaac cgcgtcagtt tcgatttaat
    10081 ggcggaaact ttggcaaaaa ccaatttagg gctgctgaag gaaggcgatt gcgtcaacat
    10141 cgaacgggcg gcgcgtttcg gagacgaaat cggcggacac gtcatgagcg gacacattat
    10201 ggcaaccgtg cctattgtcg aaatcgaacg ggacgggtac aaccgcacgg tttggttttc
    10261 gctcccgcac gaactcaaac cctatatcct gaccaaaggg ttcgtcggct tggacggttg
    10321 cagcctgacc ataggcaaag tcgaagacag ccgtttcaat gtccatttga ttcccgaaac
    10381 tttggaacgg acgctgttcg gcagcagaaa ggtcggcgac agaatcaaca tcgaaatcga
    10441 tccgaatacg caggcaatcg tcgataccgt cgaacggctg atggcgcaaa gatatgcaaa
    10501 gtgaggtaga gatggaattg aacgaatttc tcgataaagc ctatgccgtt ttgcggcgtt
    10561 tggatgccgt gcttccgccc gaacccgggc atacggattg gaacgcgctt gccttccgct
    10621 ggcagagtgc gggcaaaaaa ggttttttgg aacacttgcc cgatccgcac accttcccct
    10681 tggcaaggct ggcgggagtt ggcaggcaaa ccgaattgct ggtgcgtaat accgaacaat
    10741 tcattgccgg cagacccgcg aataacgtat tgatgagcgg cgcgcgcgga acaggcaaat
    10801 cctcgctggt caaagccctg ctgcacgaat atgcggataa gggattgcgc ctgatcgaag
    10861 tcgataaaag cgatttaatc ggcctgcctt acctgttgac gcttttgaaa gaacgtccgg
    10921 aaaaatttat cgtattttgc gacgatttgt cgtttgaaag cggcgatgaa acctataagg
    10981 cattgaaaac cgcgttagac ggcggtctgt ctcaacgttg cgccaacgtt ttggtttacg
    11041 cgacttccaa cagacggcac ctgatgcccg aatattttga cgagaatgcc ggtacgacgg
    11101 ggatgcgggg ggaaatccat cagaaagaag cggtggaaga aaaaatatcc ctgtccgacc
    11161 gattcggatt gtggctcagt ttttatccgt tcgatcaaaa cgattatctg gcggcggtgc
    11221 aaaattggct ggaagatttc ggcgtgccgt atgatgaaac cgcacagatg gcggcattgc
    11281 agtgggcgca gacgcggggc agccgttcgg gacgttccgc gtggcagttt gcctgcgact
    11341 gggcgggcag gctgccgaaa caacgggcgt tgtgagcgtt tcggggatta tttgaggcag
    11401 gaagaaagcc acatttgctg ctgttgcggt gtgaggatat ggaagaaccg gtgttggatt
    11461 tccaattcgt ccaccgcaaa atccataccg gacaaatagc ggctttcgac ataatcgcgc
    11521 gcctcgttcc gattaaaaac atccgaggaa atgatttcga cgacagaccg gcggcggctg
    11581 tgttcggaat gcataacctt caaacgcgcc ctgtcgcccg ccattttgaa ggcggcgcgg
    11641 attttacgca gttcattgtg ctgaccctgt gtcagtccga gccggcgtat gtcgcagttc
    11701 ggttgaaaat cgttcagggg gatataagcc gtggcggcgt gggaaagctg gcaggaaagc
    11761 aaagccatac ttaaaaaaga ggcggcaggt ttggcaaaac ggcagggagc aggcagtggc
    11821 acggcttttc ctcttgttgg aaattttagg aaatatacta gattgacgtg tgaaagatgg
    11881 tattatcgcg gttaactttt gttaataatg cgtacgtatg tccggcaggc agacataacg
    11941 gcggaatcgg aacgtatagt g
//
LOCUS       DAWFLT010000060        11039 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWFLT010000060 DAWFLT010000000
VERSION     DAWFLT010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11039
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.060"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            143..1540
                     /gene="aspA"
                     /locus_tag="WGR74_001557"
     CDS             143..1540
                     /gene="aspA"
                     /locus_tag="WGR74_001557"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ0905945.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGKLGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1612..2487)
                     /locus_tag="WGR74_001558"
     CDS             complement(1612..2487)
                     /locus_tag="WGR74_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0905946.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWWLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAVLLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFLLAGVLMFACLFAKSVRWRVESVVGGILLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWAEVKALFGI"
     gene            complement(2489..3211)
                     /locus_tag="WGR74_001559"
     CDS             complement(2489..3211)
                     /locus_tag="WGR74_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ0905947.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPKAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            complement(3234..3713)
                     /gene="ybaK"
                     /locus_tag="WGR74_001560"
     CDS             complement(3234..3713)
                     /gene="ybaK"
                     /locus_tag="WGR74_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ0905948.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPVQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPRDLNILNPKTIQAAV"
     gene            3788..4150
                     /locus_tag="WGR74_001561"
     CDS             3788..4150
                     /locus_tag="WGR74_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0905949.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWCVEIKITAVKKDAATA"
     gene            4185..5048
                     /locus_tag="WGR74_001562"
     CDS             4185..5048
                     /locus_tag="WGR74_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ0905950.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATIKRLRMFVLRAKVVFEILEDYAVDAEL
                     EASAEPLAAQEPSLVFTAECVSDGICTVVLPHRGILHIAPKNALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEETGIVLDSVQDSENFTALAVIKFSAAQKELTAPNGSIFKA
                     VHPFFKTENAE"
     gene            5146..6105
                     /gene="ttcA"
                     /locus_tag="WGR74_001563"
     CDS             5146..6105
                     /gene="ttcA"
                     /locus_tag="WGR74_001563"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ0905951.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIAPQKAERKVINILANKPKTCGA"
     gene            complement(6199..9138)
                     /locus_tag="WGR74_001564"
     CDS             complement(6199..9138)
                     /locus_tag="WGR74_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HEZ0905952.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRAADENPELLFAA
                     KQYSDNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFV
                     IVDEPHKFPTRESAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSGGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIHAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFTK
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGIRTAVNEAYINNGLMLSRRIDSIEDEDFIR
                     FNTMTYREFLEKLAQTAKIRMQTLHQAFYRIRNELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADLGRFEDTEHRPAAGYLFDEIF
                     YDSDIEHENVANNQIEGVTVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(9151..10842)
                     /locus_tag="WGR74_001565"
     CDS             complement(9151..10842)
                     /locus_tag="WGR74_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0905953.1"
                     /translation="MNWLGKSYARLLRNLPPETLISEDKTHNAKPENAGSQNLLIRGD
                     NLEVLKHLKNAYTNSVKMIYIDPPYNTGSDGFVYQDDRKFTPAELACLANIDEDEAAR
                     ILDFTDKGSNSHSAWLTFMYPRLYIARELLKDDGVIFISIDDNEAAQLKLLCDEVFGE
                     GNFIKDLIVNTSEGGGNAKYVVNGHETVLVYAKDITHFDNLKRPKDIRGKKIVINDEL
                     YWIQEDAVREQFGKYGNLHYEDIIEIKGIEFKNKIDKDIENNEYILIPKSYGKTIIGK
                     LRKVSDDFSKFHSILNIGNISKNLTKDGIRELEELFETGKGYSPFETPKPLDLLKRLV
                     LSATFKGNQTDLILDFFAGSGTTAHAVMQLNAEGQNGNRRYICVQLPEKTAEKSEARK
                     AGYPTIFDITKARIEKAAAKIRVEHPDYTGDSGFKIFQTADNFRQHPDKDFSPEQPDL
                     PLNDELSEEQLQTLLTTWTLYDGAALTTPVEPVRLGAYTAYLCEKRLYLMNAGFTSAD
                     LLAFIRKLDDDADFNPNRVIVFGSNMASAMQHELDQAVRGYANKKEIELNVVIRV"
BASE COUNT         2654 a         2955 c         2599 g         2831 t
ORIGIN      
        1 acccgaaaac aacgtttccc gttttcagac ggcatacgat atggacaatt tcatccggcc
       61 ggtatagtgt tcgtcctgcg gcggcatctt tccgatggcg cattttttca atattaatta
      121 tccgacttac aaaggtaaaa acatgactgt ccgtatcgaa cacgatttat tgggcgaccg
      181 cgagattccc gccgaagtgt attggggcat ccacactctg cgcgccattg aaaactttaa
      241 aatctccaca caaaaaattt ctgacgtgcc gcagtttgtc cgcagtatgg tgatggtgaa
      301 aaaggcgacg gcacaggcaa acggtaagtt gggcgcggtc aagcccgaaa tcgccgccgc
      361 cattgaaaag gcttgcgacg aagttctgct gaacaaccgc tgcctcgacc aattcccgtc
      421 cgatgtgtat cagggcggtg cgggtacttc ggtcaatatg aacaccaacg aagtcatcgc
      481 caacctcgca ttggaagcct tgggatacga aaaaggccgc tacgacatcg tcaatccgat
      541 ggatcacgtc aacgccagcc aatccaccaa cgatgcctac cctaccggct tccgccttgc
      601 cgtgtattac agtatcggcg aattgctcga caaactgact gtcctgaaaa acgcctttgc
      661 cgccaaagcc gaagaattta aagacgtttt gaaaatgggc cgcacccagc ttcaagatgc
      721 cgtgccgatg actgccggcc aagaattcca atctttccaa gtgttgttgg aagaagaaat
      781 cctcaacctc gaccgcaccc gccaactgct gttggaagtc aatttgggcg caacggcaat
      841 cggcacgggc gtgaacacgc ccaaaggcta cgccgaactg gtggtcaaaa aactctccga
      901 agtcagcggc ttgccttgca aactgaccga aaacctgatt gaggcgacct ccgactgcgg
      961 cgcatatgtg atggtacacg gcgcattgaa gcgcacggcg gtcaaactct ccaaaatctg
     1021 caacgacttg cgcctgctct cttccggccc gcgtgccggc ttgaaagaaa tcaacctgcc
     1081 cgaattacag gccggttctt ccattatgcc tgccaaagtc aatcccgtga ttcccgaagt
     1141 ggtcaaccaa gtctgcttca aagtcatcgg caacgacacg acgattacct tcgccgccga
     1201 agccgggcaa ctgcaattaa acgtgatgga gccggtcatc gcccaatgta tgtttgaaac
     1261 catttccctc ttgggcaatg ccgcagtcaa cctgtccgac aaatgcgtca aaggcattac
     1321 ggtcaaccgc gaaatctgcg aacgttacgt tttcaactcc atcggtttgg tgacttatct
     1381 gaatccgtat atcggacacc acaacggcga tttggtcggc aaaatctgcg cccaaaccgg
     1441 caaaggcgtg cgcgaggttg tcttggaacg cgggctgttg agcgaagaag aactcaaccg
     1501 catcctctct cctgaaaacc tgatgaaccc ccatctgtaa cgttgcagac aaatgccgtc
     1561 tgaagcaggg atgctgcttc agacggcatt ttgttatatc aatcagccgg attatatgcc
     1621 gaacagtgcc ttgacctccg cccaataaaa caggcaggcg atggaacaca gtgccaacac
     1681 gattcccgcc gtattgattg tgttgatttt ttccttaaaa aacagtgcgc ccgaaagcgt
     1741 ccccaaaaca atcacgccga tattcatacc ggcaaaaacc agcgtgggat tgtccttcat
     1801 catttggtgc gcggtgatgt aggttacgat gttcatgaag ttcaaaccgc ccaagagtat
     1861 gccgccgacc acactctcaa cgcgccatct gaccgatttg gcaaacaggc aggcaaacat
     1921 cagcacaccc gccagcaaaa atgcaaccag caggttgccc gcaaatgccg ttccgctttt
     1981 ggcaagctgt ttgaacagga tatcgataat gccgtaacct gcccacacgc ccagcagcaa
     2041 taccgcctgc cgccacgcgc ttcctgattt tttgccaccg ctgtgtttcc aaagcaggca
     2101 gaacagtgcg gcaaatgcga ggcacagccc gattagtctg ccttcgctga gtttttcgcc
     2161 aaacagcgtc aaggcggcaa caatcggcaa aaacagcgac aaacgctgcg ccgcgtcgga
     2221 tttgacgata ccggcggatt ctacggattt gcccataatg acaaatacgg acggcagcag
     2281 cacgcccaat gcggcaaaca gccaccatgt cggcaaaaat gcgccgatat tgccgacatc
     2341 cggcttcaat accagcaggg tcagtatgac cgcgaccaca taattgacgg cgaccgcctg
     2401 cgcgatgtcg atttttttct tccttgccat tttcagcaaa atggaaaccg acacgctgaa
     2461 cacgatgctg attaacaggt aagccatact atatccattt ctcgggtgca accgccctgc
     2521 cgccgttgaa gcggataaag ccggcgatga tgcggtagaa ataccagacg ttcgttgcaa
     2581 taataatcag cacgccgata cccaaaaggg cagtcagcgc acccaaaata taaagccaaa
     2641 atgtgcgcca aaaggtttta atcaggtatt cggtatgtgc ggcatagaca atgctgtcca
     2701 aactgttccg tttcatatag gcgagaatca cgccgactat gggcatatag gcgaaaatca
     2761 cgccgactat gggcatgaac agcatgattg cacccaaacc gaacaggatg taggcggcga
     2821 ggacatagtt gcgcccgctt ccgccgccgg acggttcgta agtatggcac gaacgtgatg
     2881 cctgccgttc aaacgcctgt ctgaacgcct gttcgcggcg catctgttcg cggtaaaact
     2941 gctccctgcg cgcctgttcg cggcggaatg cctcttcctg ccgcccgcgt tcctcatgcc
     3001 gtctgaagga cgcgtcgtat tgcatccgtt tcgacaggtc ggaaagcgta tcgtaagccc
     3061 gttggatttc tttgaacttt tcttccgcct tcggattgcc cggattgcgg tcgggatgat
     3121 atttcatcgc cagcttgcgg taggcgcgtt tgatttcgtc cgctcccgcc tgcggcgaca
     3181 cgcccaatac ggcatacaaa tctttgtcca tattgttcct ttatactttc ccgtcaaacc
     3241 gccgcctgta ttgttttcgg gttcaaaata tttaaatctc tgggacggat gccgataatg
     3301 aacccgcgtt ttccgccgtt gatatagatg gtttccaaat ccatcaccga ctgttcgacg
     3361 taaatatcca actttgtccg gatgccgaac ggcgttgtgc cgccgaccag atagcccgtc
     3421 cacttgtttg cctgtacggg cgtggcgggt tcaatgtgtt tcgcacccaa atgccgcgcc
     3481 agattgcggg ttgaaatctg cttgtcgccg tgcatcaaga caatcagccc cttaccgttt
     3541 tcatcttgca aaacaatggt tttaatgacc aagtgttcgt ctttgccgaa tagtcgggca
     3601 aactgcgccg tgccgccgtg ttcctcatag gcataggtaa aaggttcaaa ttcgatgccg
     3661 ttttcacgca aaacgcgcac ggcaggtgtt atcggataag tgtgtttact catttttcgt
     3721 catgcaaaac atggaaatcg gtcaagcgca atgttaccat atatcccctt tatcgaagga
     3781 aaccgatatg gatatccgat atttcggcac aacgccccgc tattccgaag ccgtcggcgc
     3841 aaacggcttg attttcctct ccggcatggt tcccgaaaac ggtgaaacgg ctgccgaaca
     3901 gactgccgac gtgcttgccc aaatcgaccg ctggctggcg gaatgcggct cggacaaagc
     3961 gcacgttttg gatgcggtca tctatctgcg cgatatgggc gactatgcgg aaatgaacgg
     4021 cgtgtgggac gcttgggttg ccgccggcag aacgcccgcc cgcgcctgcg tggaagctcg
     4081 gctcgcccgt ccggagtggt gcgtcgaaat caaaataacc gccgtgaaga aagatgcggc
     4141 aaccgcataa acagatttcc tttatatttc agagtacgca aatgatgaaa accttactgc
     4201 cctttttcgg cgtggtacgc gtcagcggcg aagaccgcca aaccttcctg cacggacagc
     4261 tttccaacga catcaataat cttcagaccg ggcaggcgtg ttatgcgact tacaacacgc
     4321 ccaaaggccg cgtcatcgcc aatatgattg tcgtcaaccg aggcggcgat ttgctgctga
     4381 ttatggcgca agacttgctt gaagcaacga tcaaacgcct gcggatgttt gtcctgcgcg
     4441 ctaaagtcgt ttttgaaatc cttgaagatt acgccgtcga cgcggaattg gaagcatccg
     4501 ccgaaccgct tgccgcgcaa gaacccagtc tcgtctttac cgccgaatgc gtttcagacg
     4561 gcatctgcac cgtcgtcctg ccccatcggg gcattttgca catcgccccc aaaaacgcct
     4621 tgccgcctta tgatgccgcc gccgaaaacg cgtggcggct gcacgaaatc cgcagcggtt
     4681 acccgtggat atgcgcggct accaaagaaa ccgccgtcgc ccaaatgctc aaccagcaca
     4741 tcatcggcgg cgtacacttc aaaaaaggct gctatccggg acaggaaatc attgcccgtg
     4801 cccagtatcg cggtcaggtc aaacgcggct tggcggtatt gtcgggaaat tcggccgtcg
     4861 aagcaggaac cctgctgacg gcagacggcg aagagaccgg catcgtactc gacagcgttc
     4921 aagattcgga aaactttacc gcccttgccg tgattaaatt ctccgccgcg caaaaagaac
     4981 tgaccgcccc aaacggcagt atcttcaaag cggttcatcc cttctttaaa accgaaaatg
     5041 cagaataatc cctctaatgc cgtctgaacc tttttcagac ggcattttaa aaccgcccct
     5101 gttttcggtt acaatgccgc atccccaatt tttcaaacca ccgccatgtc caaaaaaacc
     5161 aaacaagaac tggaaaacaa caaactcagc aaacgcctgc gccacgccgt cggcgacgcg
     5221 attaacgatt tcaacatgat cgagccgggc gacaaaatca tggtctgcct ctccggcgga
     5281 aaagacagct atgccctctt ggacatcctg cgccagctcc aagccagcgc gccgattgat
     5341 ttccaactgg ttgccgtcaa tctcgaccaa aagcagccgg gcttccccga agaagtatta
     5401 ccgacctatc tcgaaagcat cggcgttccc tacaaaattg ttgaagaaga cacctactcc
     5461 accgtcaaac gcgtattaga cgaaggcaaa acgacttgtt cgctgtgcag ccgcctgcgc
     5521 cgcggcatcc tctaccgcac tgcaaaagaa ttgggctgca ccaaaatcgc cttgggacac
     5581 caccgcgacg acatcctcgc caccttgttt ttaaatatgt tttacggcgg caaactcaaa
     5641 gccatgccgc ccaagctggt gagcgacaac ggcgaacaca tcgtcatccg tccgctggcg
     5701 tatgtaaaag aaaaagactt aatcaaatac gccgaattaa aacaattccc catcatcccg
     5761 tgcaacctct gcggctcgca acccaacctg caacgccaag tcatcggcga tatgctgcgc
     5821 gattgggaca aacgctttcc cggccgcatc gaatcgatgt tctccgccct gcaaaacgtc
     5881 gtcccttccc accttgccga taccgaactc ttcgactttg ccggattgga acgcggtcaa
     5941 accctgaaac acggcggcga tttggcgttt gacagcgaaa aaatgcccga acgtttttca
     6001 gacggcagcg aagaagacga aagcgaaatc aaaatcgcgc cgcaaaaagc cgaacgcaaa
     6061 gtcatcaaca ttctggcgaa caagccgaaa acctgcggcg cgtaaccccc cgccgtcaaa
     6121 aatgccgtct gaagcccgtt tggcgtttca gacggcattt taaaatcccg aagcagacgg
     6181 cttccaaaca tttgtttctc agtgtccgtt ttcagaatgc ccgcctgcgg gcatattttg
     6241 cccgatcagt tcggctatcc tctcgccgtc aaactgcgtt ttgaacacca ccttgatttc
     6301 tttggaaatc tcggcaaaca gcttttcggc atgtttgatt ttccgctctt cgctttttcg
     6361 caaatcttcc gccccgtcag tccctttggc ttcaatcaca aagttcagga tctcgccgct
     6421 tttggttttc acgatatagg caaaatcggg cgaatacgtg ccgccgccgg caacagggat
     6481 tttgatggag tttctcggta ttttggtaaa tacggttacg ccttcaattt ggttgttggc
     6541 gacattttca tgttctatat ccgaatcgta gaaaatctcg tcgaagagat agccggcggc
     6601 aggccggtgc tccgtatctt caaatctgcc caaatctgct tttttcaccg cgcgcggttt
     6661 gccgtcttta ttggtaaatt tggtcggatg gattttgctg ccgacaagcc ggtaatccag
     6721 ttcgaattta tggaaggaat gatgaagcaa aaaccggttg aagccgtttt tgatttgggc
     6781 gatggtttgc atattcaaaa aatcgccaat gttcagttcg ttgcggatgc ggtaaaacgc
     6841 ctgatgcaaa gtctgcatac ggatttttgc cgtttgtgcc agtttttcca gaaactctcg
     6901 gtaagtcatt gtgttgaaac ggataaaatc ttcatcttca atactgtcta tgcggcggga
     6961 aagcataagc ccgttgttga tataagcttc gtttaccgcc gtgcgtatgc ctgcctgcgg
     7021 gaatttggcg gcgttttcac gcaaataggc ggtaaataaa tcgacaaatt cggcttcatc
     7081 cttgattttg tactgcaaaa cggctttatg gtgaatcagc tcccacaagg ccttgagttc
     7141 ttcatatttg ccttcgcgca tgatgatggt gtctttgccc tcgtctttgg cgttgctgac
     7201 tttgcctttg tccaaacctt tggggaaggc ttcgggatag gcggctttta atttgtcata
     7261 gccgtctttg gtaaagtttt cattgtcgtc aatgatgccg tctgaaaaca gttggtttac
     7321 caataccagc ggtttgatat cggggtattt ttgcaatatt ttttgtttca gctcttcggt
     7381 aaactttttg gagatttctt cctgaaaaga attatcgttg atttcgccga caagctgctt
     7441 cacaaagtct ttttcgctgc tatcgacaaa ataattcagt ttgtacggca catcgcgcac
     7501 ccgcgccatc agctcgttta ccggcaggcg caggccgcgt ccgacttctt gcagcttgga
     7561 agtcgtgctg ccgctggaac gcagtttgca aatctggaaa acgttgggat tgtcccagcc
     7621 ttcgcgcagc gtccatttgg aaaaaataaa gcggcgcggg ttgtccaaag acaacagttt
     7681 ttccttatcg tgcaggattt cattgatttc ctgctcgatt ctatcgtcgc tgtctgtatt
     7741 gtctttggaa aaataaccgc cgtggcaggc ggatacatcg tccaacgtct tttgcaggta
     7801 atcgcggtaa aacgggtcgc tttccgtttt caggcggcgt gccgcttccg cgtgaatcca
     7861 gctttcaaat ttatctttca ggctgcctga aagctcgttg ccgctgcggt agcccgcaat
     7921 atcatcaata aaaaacagcg tcagcggctt gattttgggc tgtggcgcgc gttctgccaa
     7981 aagcgcgcgt tccagcttga aatgttcggc aaccgcccgc tgcatcatcg catcctgcac
     8041 cgtttgcgaa taggaataag ggttgatgac ggcacccgtt ttcaactcca agccgttgct
     8101 taacaccacc acggttttat tcattttgtc gattttcaaa tccgaaatag ccggatggat
     8161 ttgcgccaaa tcttcgcctt ttgccagttt gaacgtctgc tttttgtcct tttcgtttaa
     8221 ttcaaatttc gcttctttgc cgcccgacga caccagtttt accgccgcat ccatgccgcc
     8281 ctgcatttct tcctgaaaca cgcgcacgcc tttgaccagc ccgtcgttaa acgcgtctac
     8341 tgccgtcaaa cggtaaagca agttgtaata ttcatcgtta aatgtcgcac cgtagcgcaa
     8401 aatatattgc ggttttaagc gtttgatatt gccccacgtt ttcgcgctct cccgggtcgg
     8461 gaatttatgc ggttcgtcca caatcacaaa cgggcgcacg gcggccaatg catcaacggg
     8521 attgtcaaac aaatctttca atgccttgtc gcccgtatcg ttcatggacg acgaattaac
     8581 catgcccgcg ttaatcagca gcacatggat ttcctttttg ttttccgctt tgacaaattg
     8641 ctcaatcgtt atgggcgcat tggacttttt gcccttattc tttttcgcgc tttccaccac
     8701 ataggttttc aggcgcacgc cttcataatc gccgccgaaa tcctgttcaa aatgctctgc
     8761 caaagccttg ctttgcaaaa actgctgtgt tcccgcctta atggacaaag tcggcacgac
     8821 cacgataaat ttgaacacgc ccagccaacg gtgcagctcg aacatggttt gtgtgtaggt
     8881 ataggttttg cccgtgcccg tttccatgga aatatcaagg atattttggt cgtccgaacg
     8941 gtcggggaat cggccgtcta taccgttttg gctttgcact ttcaggatat tgtctgagta
     9001 ttgttttgca gcaaacaaaa gttcgggatt ttcgtctgcc gcccgatatt tgggcgttgc
     9061 cccgtcaaac acgcccaaaa ccgccgaaac cgcccgcatt tggtgcggct ggtttttctc
     9121 gtaattaaaa ccgctcatga attgcctccg tcaaaccctg ataaccacat tcaactcaat
     9181 ctctttttta ttggcataac cgcgaaccgc ctggtcaagt tcgtgctgca tggcgcttgc
     9241 catattgctg ccgaatacaa tcacgcggtt gggattgaaa tccgcatcgt cgtccagctt
     9301 gcggataaac gccaacaaat cggcggaagt aaaaccggca ttcatcagat acagccgttt
     9361 ttcgcacaga tacgccgtgt aagcccctaa ccgcacaggc tcaaccggcg tggtcagtgc
     9421 cgccccgtca tacagcgtcc aggtggtcag aagcgtttgc agctgttctt cgcttaattc
     9481 atcgttaagc ggcaaatccg gttgttcggg cgaaaaatcc ttgtccggat gctgcctgaa
     9541 attgtctgcc gtttgaaaga ttttgaagcc cgaatcgccc gtgtaatcgg gatgttcgac
     9601 gcggattttg gcggcggctt tttctatgcg ggctttggtg atgtcgaaga tggtcgggta
     9661 gcctgcttta cgggcttcgg atttttcagc ggttttttcg ggaagctgta cacagatata
     9721 gcggcggtta ccgttttgtc cttcggcgtt aagctgcatc acggcgtggg cggttgtgcc
     9781 gctgcctgcg aagaagtcta ggattaggtc agtttgattt cccttgaaag ttgcagaaag
     9841 aactaatctt ttcaataaat ctaatggttt tggtgtttca aaaggagaat aaccttttcc
     9901 agtttcaaac aactcttcta attcacgaat accatcttta gtaagatttt tacttatatt
     9961 tccaatattt aagatgctat gaaatttgga aaaatcatcg gatacttttc taagttttcc
    10021 aataattgtt ttcccataag acttcggaat taagatatat tcattatttt caatatcttt
    10081 atcaattttg tttttaaact caattccttt tatttcaata atatcttcgt aatgcaagtt
    10141 cccatatttg ccaaactgtt cgcgaacggc atcttcctgt atccaatata attcatcatt
    10201 gattacaatt tttttgccgc gtatatcttt tggtcttttt agattgtcaa aatgagtaat
    10261 atctttcgca taaactaaaa cagtttcatg cccatttaca acatattttg cattaccacc
    10321 accttctgat gtattaacaa ttaaatcttt aataaaattc ccttccccaa acacttcatc
    10381 acacaacaac ttcaactgcg ccgcttcgtt atcgtcaatc gagataaaaa tcacgccgtc
    10441 gtcctttaac agttcgcggg cgatatacag gcgcggatac ataaaggtca accatgcact
    10501 gtgcgagttt gagcctttgt cggtgaaatc taaaatccgc gcggcttcgt cttcgtcaat
    10561 attggctaag caggcaagtt cggctggtgt gaatttgcgg tcgtcctgat agacaaagcc
    10621 gtctgatccg gtgttgtagg gcgggtcgat gtaaatcatc ttcacgctgt ttgtgtaggc
    10681 gttttttaag tgtttcaaca cttctagatt gtcgccacga atcagtaggt tttggctgcc
    10741 tgcattttcg ggtttggcgt tgtgcgtctt gtcttcactt atcagggttt cgggcggcag
    10801 gttgcgaagc aggcgagcat atgatttgcc cagccagttc atttcgtaaa attcgcgtcc
    10861 gatgtcggtc tgtggcgcaa tttcggcttg taatctgtcg ataaggaaat ttccgtctgc
    10921 gtcaaaacag gcgggaaaca gttttttgag ctgttcgagt tgggtagagt tggcggtaat
    10981 gccgtctgaa gtgtagattg cctcggtgtt caccccggct gtgtcgatta gatcagggg
//
LOCUS       DAWFLT010000061        11002 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWFLT010000061 DAWFLT010000000
VERSION     DAWFLT010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11002
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.061"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..126)
                     /locus_tag="WGR74_001566"
     CDS             complement(<1..126)
                     /locus_tag="WGR74_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0905954.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDRVIDWQPIEQYLNRQR"
     gene            complement(189..697)
                     /locus_tag="WGR74_001567"
                     /pseudo
     CDS             complement(189..697)
                     /locus_tag="WGR74_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(807..1094)
                     /locus_tag="WGR74_001568"
                     /pseudo
     CDS             complement(807..1094)
                     /locus_tag="WGR74_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1202..1411)
                     /locus_tag="WGR74_001569"
                     /pseudo
     CDS             complement(1202..1411)
                     /locus_tag="WGR74_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1408..2463)
                     /locus_tag="WGR74_001570"
     CDS             complement(1408..2463)
                     /locus_tag="WGR74_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0905955.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            complement(2569..3051)
                     /locus_tag="WGR74_001571"
     CDS             complement(2569..3051)
                     /locus_tag="WGR74_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0905956.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            complement(3098..4234)
                     /locus_tag="WGR74_001572"
     CDS             complement(3098..4234)
                     /locus_tag="WGR74_001572"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ0905957.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPNPNA
                     PAAEFADRHLCAPFDNQTALEELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLSTDSDTAFQEAKKLHQSL"
     gene            complement(4231..4776)
                     /locus_tag="WGR74_001573"
     CDS             complement(4231..4776)
                     /locus_tag="WGR74_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ0905958.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKSREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRSAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            complement(4773..6248)
                     /locus_tag="WGR74_001574"
     CDS             complement(4773..6248)
                     /locus_tag="WGR74_001574"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ0905959.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIRAAQMVQEGLDK"
     gene            complement(6359..6724)
                     /locus_tag="WGR74_001575"
     CDS             complement(6359..6724)
                     /locus_tag="WGR74_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905960.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            complement(6745..8655)
                     /locus_tag="WGR74_001576"
     CDS             complement(6745..8655)
                     /locus_tag="WGR74_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0905961.1"
                     /translation="MNILSVENASFAVGHVALLDKTSFQLDRGEKIGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIVQNNLKIVYVPQESFFDKEATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFTYGDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     TLNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFHPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILILDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     IFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            complement(8674..9318)
                     /locus_tag="WGR74_001577"
     CDS             complement(8674..9318)
                     /locus_tag="WGR74_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0905962.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            complement(9482..10300)
                     /gene="opc"
                     /locus_tag="WGR74_001578"
                     /gene_synonym="opcA"
     CDS             complement(9482..10300)
                     /gene="opc"
                     /locus_tag="WGR74_001578"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HEZ0905963.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWLAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTNLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
     gene            complement(10889..>11002)
                     /locus_tag="WGR74_001579"
     CDS             complement(10889..>11002)
                     /locus_tag="WGR74_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0905964.1"
                     /translation="ARAAYFGLIKVSAQSHLKAMCLNLLKAANKLSAPAAA"
BASE COUNT         2367 a         2421 c         3125 g         3089 t
ORIGIN      
        1 tctttgacgg ttcaggtatt gttcgatcgg ctgccaatca atcacccggt ccaacttcaa
       61 tagcgggaaa cggtcgatgt gtttggcaat catggcttgt gcggtttgcc ggaagaaggt
      121 gctcatggaa aatcccctaa atggcttggt gggaatttag gggattttgg ggagttttgc
      181 aaaggtctcc ggttgcgttt ggtttgcgct tccgtcagcg gacggttgcg gcaggctttg
      241 cgcataatgc cgtcctgcaa ctgatgttct tccagatgtt gccggttttc cgcactgtcg
      301 tagcctttgt cgacatagac ggtcgtacct ttgggcagtc cttccagcaa aggcgacagg
      361 tgtttgcact catgggcatt ggcgggggtg atgtgcagtt tctcgatata gccttctgca
      421 tcggtacgtg tatgttgttt gtaaccgagt ttgtagaggc cgtttttctt tgtccaacgg
      481 gcatctttat ccttactcgg tgtggtttgg ccgttgattt gtccttcttc atcgacttct
      541 atagcctgac gctgtttgct gccggcggtc tgaataatgg tggcgtcaat gctttctcta
      601 tttttaaacc tttttcggtc agttggcggt taatcagttc cagcaattcg gacagggtgt
      661 cgtcttgcgc cagccagttg cggtagcggc ataaagtata gtggattaac aaaaatcagg
      721 acaaggcgac gaagccgcag acagtacaaa tagtacggca aggcgaggca acgccgtact
      781 ggtttttgtt aatccactat actgctcagt tcgtcgaaac ggcaaaacag gttgaaatcg
      841 atgcgggtaa tgaggctgtg ttcgagttcg ggatcggaga ggctgtgcca ttgtccgagc
      901 aggacggctt tgaacatgga caacagcggg taggcgggac ggccgcggtg gtctcgaagg
      961 taacgggttt tttgacggtt caggtactgc tcgatcggct gccaatcaat cacctggtcc
     1021 aacttcaata gcgggaaacg gtcgatgtgt ttggcaatca tggcttgggc ggtttgttga
     1081 aagaaggtgc tcatgagaaa tcccctaaat gtcttggtgg gaatttaggg gattttgggg
     1141 aattttgcaa aggtctctgg ttttgtttct gacaggcaaa agcactgttt atttggctgt
     1201 caaaaggatg gttaaggaaa gttatgcgcc cctgaagcgg gccccagata aggatggttg
     1261 cgccgacggc ttcagacggc attttggcgg cggtgttggg ttttgtatcc ggtttgccgt
     1321 tgtgtcctgt gatgatgatt ttgggcgcgg tttttctgtt ttgatgtgtg aaatgccgtc
     1381 tgaaaggcgg ttcagacggc atagcggtca tttttgtgcg gtcaggcggt cgaatatgcc
     1441 gccgtcggcg aagtaggttt tcatgatgtt gtcccatccg ccgaattttt cttcgggaga
     1501 gaaggtgtct aagtctggga agtcggcttt gtgtcttgcc aatacttcgg ggttgcgggg
     1561 gcgcaggtag agtgaggcgg cgagttcttg cgccggttcg ctccaaaggt attcgagata
     1621 ggcgcgggcg gttttttgcg tgcctttttt cgcgacgacg ctgttgacga cggcgacggg
     1681 gctttcggcg gaaatggtgt agctcggata gacgatttca aactgtcctt gggtcagttt
     1741 tttgctgacg tagttggctt cgttttcaaa agtgatgagt acgtcgccga tgttgcgttg
     1801 tgtgaaggtg gtggtggcgg cgcgtccgcc gttttcaaaa acgggggtgt ttttgaggat
     1861 ggatgcgacg agtttttggg cttcctgttc gttgccgttg gtggttttca gaccgtaacc
     1921 gtatgcgccg aggaaggcgt agcgtccgtt gcccgaggtt ttgggattgg cgatgacgat
     1981 gttaacgccg tctttggcaa ggtcgttcca atcgcggatc tgtttggggt tgttttttcg
     2041 gacaaggaaa accatagtgc tggtgtaggg cgcggcgtgg tcggggaggg cttgttgcca
     2101 gcctttttct accagtcctt ttttttcgag caggtcgatg tcggaggatt ggttcatggt
     2161 tacgacatcg gcttgaaggc cgttggctac ggataatgcc tgtttgctgg agccgccgtg
     2221 ggactgttgg atgctgacgg atgtgccggg gtgttcggat tggtatgttt tgataaataa
     2281 ggggttgtat tctttgtaaa aatcccgtgc cacatcgtat gaggcgttga gcagggtaat
     2341 gttttttccg tcggattcgg tattggccgg ggcattttgt ccggacggat ggtttgaatc
     2401 ggctgcgggg ctgcaggcgg tgagcagggc tgcggtatag agtgccggtg cgtaggtttt
     2461 catatgcttg tcctgtcggt tggtagatgg ggcaacttta tacggctgtc tgcgcttgtg
     2521 gaaataatgt ttgatttgaa gattatcagt tttggttata aggacggatc agaggtgttt
     2581 ccgcatcagt tcgcatttga ttttgatgct ggggtcaagc tgcaatactg ccgaaccgag
     2641 cgattcgtag cggttgagaa ggtaaaacgg gacggagttg agcgatgcgt agagtttcag
     2701 aaagctcaaa ccggatttgt gggcgatggt ttccgcctga tgaagcaggg cagtgcccag
     2761 tccgaggttg tggaacaggg ggtggacgta aagtgcatcg agttgtgctt cttggcagtc
     2821 gatttggaaa aatccctgta tgttgccttt gtattcggca acccaaagtg ctttgtcggg
     2881 atcggaaatg gtcggcaggt agctttctgt gtttaacaag ccttcccata cttttagggc
     2941 gtgttcgttg tagctgagga tgcaggtgta ttggacggag tgcaggtgga ctttgaagat
     3001 gtctttgcag tcttgcacgg tggcggggcg taacagtgtc agcaggttca tggcagtatg
     3061 tcggcggctt cagacggcat ctgtgccgtt ggtcggatta tagggactga tgcagttttt
     3121 ttgcttcttg aaatgcggtg tccgaatcgg tgcttaaaac ggtaaagtgt cccattttcc
     3181 gccctttgtg cgcggttttt ttgccgtaaa ggtgcaggtg cgcgtccggc cggctttgca
     3241 ggggaaacca atccggttcg ccgccgtctt cctgccaaac gtcgcccaaa atattcgcca
     3301 tacagcaaga actcagcaat ttggtgtcag caggtggcag gttgcacatc aggcggacct
     3361 gttgctggaa ttggtctgcc gcgcaggcgt cgacggtatg gtggccggaa ttgtgcggac
     3421 gcggcgcgat ttcgttgacg accaattcat gcgtgtcgcc gacaacaaac atttctaccg
     3481 ccaatacgcc gacgtagttc aattcatcgg ccaaacgctg cgccatttgt cgcgcctgtt
     3541 gctgaatgtc ggcactcagt cgggctggga cgatggagta ggcgaggata ccgttttcgt
     3601 gaatgttttc ggcaggatcg aaagtttgca cgttgtcatt gttcagacgg catacgataa
     3661 cggaaatttc gccgcgcaag tccaccattt tttccaaaac gcaatccacg ccgcggtgtt
     3721 cggcaaacgc ggctttgagt tcatccaccg ttttgacgcg gatttggcct ttgccgtcat
     3781 agcccaatgt agcggttttc aggatgccgg gcagaaattg tatgctttct tcagtgatgt
     3841 cttcggcttt gcaaattgct tgatacggcg cggtttgcag gcctgctttg cgtatccatg
     3901 ccttttcctg aatgcggttt tgcgcgatgg caacgcagtc gccgctgggg gaaacattgg
     3961 tatgtttggc gagaaaacgc atcgcatcgg cattgacgtt ttcgaactcg gtcgtaacag
     4021 ccgcacattt tgccaattct tccaaagcgg tttggttgtc aaacggcgca cacaaatggc
     4081 ggtcggcaaa ttccgctgcc ggcgcattcg ggttgggatc gagtacggtt actttgtagc
     4141 ccatggtttt ggcagcaaca gtaaacattc tgcctaattg tccgccgcca agaatgccga
     4201 gcatggcggg cggagaaaga gatatgtttt tcatgctgac tcttcaaatt gtacaagttg
     4261 atagctataa ctaattcttg acggatgtct tgtatcgctg gaattaccag tttcagaaat
     4321 acagaatact ttttccataa atttttctgc ttttagaaat tccagtattc tgtttttttc
     4381 atccttataa gcactgcggt ctgtacccca tgcaagaata atcatatcag catcttccaa
     4441 acatccttta aatttggaaa aatcggtttg ggtgtttgcc ctaattcctg tttgctgtgt
     4501 agagtaactg gaaaaaatat ttaacatttt gaagttggta aaaccgtaca tatccaagaa
     4561 acgtgcaagt tgggtaaggg ttttgtcgct tctttcatca tttgctttac ttggattaat
     4621 tcctatagca acagctgaga aatttttagg attttctgtg ttgccgctcc atctaaccgt
     4681 taggatttct cgactttttt cattatctgt atagagaccg tctttggtag tcggtctgag
     4741 tgtttggcga agctcataaa gtttttcata agtcatttat ccaacccttc ctgcaccatc
     4801 tgcgcggcgc gtataacggc tcgggctttg ttttgtgttt cctgccattc ggaggccgga
     4861 tcggagtcgg caaccacgcc cgcgccgctt tggacgtata gcgtgttgtt ttttactacg
     4921 gcggtgcgga tggcgattgc caaatccatg tcgttgttga aaccccatac gccgacggca
     4981 ccgccgtaga tgccgcgttt gctcggttcg acttcttcga tgatttccat ggcgcggact
     5041 ttgggtgcgc cggagagtgt gccggcaggg aaggtagcgg cgaggatgtc catgttggtc
     5101 atgccgtctt tcagacggcc ttcgacgttg gaaacgatgt gcattacatg ggagtatttt
     5161 tcaatcacca ttttgtcggt aactttgact tcgccggttt tactgatgcg gccgacgtcg
     5221 ttgcgtccta agtcaatcaa catgacgtgt tcggcgattt ctttggcatc gcttaacaaa
     5281 tcttgttcgt tggcaaggtc ttcggcgggg gttttgccgc gcaggcgcgt gccggcgatg
     5341 gggcggacga tgacatcgtc gcgttcgcgg cggacgagga tttcgggcga ggagccgacg
     5401 atgtggaaat cgccgaaatc gtagtaaaag agataaggcg aagggttgag cgtacgcagg
     5461 gcgcggtaga gggcgagcgg gctgtcggtg aattccatgc tcatgcgctg gctggggaca
     5521 acctgcatgc agtcgcctgc gaagatgtag tctttgattt tgttaacgca ggctttgaac
     5581 ggctcttcgc cgaattcgct gacggcttcg gtgtgtttgc tgccgagcga gagcgggatg
     5641 gcgcagcttt ggcgcaactg ggtgcggatg tcttcgaggc gttcgcgggc gcgttcgtag
     5701 ccgtcgggct gcgacggatc ggcgtaaacg acgagatgga ttttgccgct caaattgtcg
     5761 atcaccgcca attcttgcga cagcatcagc aagatgtcgg gcgtgccgag cgggtcggct
     5821 ttggtggtgt ttttcaggcg gtgggcgaag tgttcaaaat tgtagatggt ttcgtaaccg
     5881 aagtagccga ccagtccgcc ggtaaagcgc ggcaggcttg ggatttcggg tgttttgaag
     5941 cggttgtgga aggcttcgat aaagggcagg gggttgccgt cgtgttgctc gacaatttcg
     6001 ccgttttgat aaacatcgac gtgtttgccg ctggctttga gatagtggct gcaaggcagg
     6061 ccgataaaag aatagcggcc gaaacgttcg ccaccgacaa cggattcgag caggtaggta
     6121 taggggcggt tggcgagttt gagatagagg gaaagcggcg tatccaaatc ggcaaggagt
     6181 tcttgcacga gcgggatgcg gttgtagcct tgggcggctt gggcttggta ttcttgtttg
     6241 ctgatcattt ctgctttccc aaagggcggt ttcggacggc gcggcaacgg gcgcgagtat
     6301 agcattttat cggaattgtt gacagtctga ccggagatgc cgtctgaaaa ggacgggctt
     6361 acggctttgt tttgcgctgc cggtattgtt tccatttaaa cagataacct gcggttacgg
     6421 gaatcagggc taagatggtt ttgaacgtcc atgatgcata ccaaggtaaa gaaccgggtg
     6481 cgaccgattg attcagatac ggccaccaac aggcagtcag gcagccgagc agcagcggcc
     6541 agatatgccg gctgaggttt ttttgccaaa ggaggaatga tgcggcagac caaagcaggg
     6601 taagggcagt gatttttaac acggtataag catccgctcc aaacagggcg gcgagaaaat
     6661 acgtaccggc aatccagagg acggcaagta tcaggacaat gatttcttca ggtcgtttga
     6721 acattttttt cttcctgttt gatttcagac ggcattgccg ttctgtttgg tttccagcag
     6781 ctcccagcgt tccagctttt ccaaaagcag catttcgatt tcttcggcgc ggctttgcag
     6841 tgcgccggct ttttcgtaat ctttgaagat ttcgggatcg gaaagctgcg cgttgatttc
     6901 tgcctgttcg gcttccaagg cggcgatttc gtcgggcagg gcgtcgagtt cgcgctgttc
     6961 tttgtaggac agtttgaccg tgcggttggc tttgggtttt tctttcaccg gttcggcatc
     7021 ggctgttttg ggtgcggatt ccgtctgaat cttcgcttcg cgtgattttg cgtcgatgta
     7081 gtcctgatag ccgccgatat attcttttaa ccttccattt ccttcaaaaa taatgctttg
     7141 ggtaatcacg ttatcgagga acatacggtc gtgtgagaca aggaataccg tgccttgata
     7201 atcgcgcagc aaatcttcaa gcagctcttg ggtatcgata tccaagtcgt tggtcggttc
     7261 gtccaaaatc aaaatattag caggacgggt aaagagtttt gccagcaaaa ggcggttgcg
     7321 ttcgccgccg gagagcgatg aaacgggact ttgtgcgcgg gctggatgga ataagaaatc
     7381 ttccagatag ctcattacgt gttttttctt gccgccgact tcgacgtaat cgttgccttg
     7441 accgagcgtg taaaacacgg tgtcgttttc gttcaacgtg ctgcggaatt ggtcgaaata
     7501 ggcaacttcc tgcttactgc cgatacggat tctgccgtag gtcggctgca attcgcccaa
     7561 aatcagcttg aggaaggtgg ttttgccgat accgtttggg ccgattaagc cgattttgtc
     7621 gccacgctgc aagatggcgg agaatttgtc catgatgact ttgtcgccat aggtaaacga
     7681 ggcatgttcc agttcggcaa tgattttgcc gcttttctcg ccgctgtcga gcttgaaatt
     7741 gacctgccct tgtacgttgc ggcgttcggc acgctggcgg cgcagttctt ccaaacggcg
     7801 cacgcggcct tcgttgcggg tacggcgcgc ttcgatgcct ttgcgtatcc acgcttcttc
     7861 ctgcgcgtgg aatttgtcga agaggcggtt gtgttccgct tcgactgcca actcttgcgc
     7921 ttttttctcg ctatatttgg agaacgagcc gggataggaa cgcaaaatac cgcgatcgag
     7981 ttcgacaatc cgcgtggcga tattgtccaa aaaacggcgg tcgtgggtaa tcacaaccag
     8041 gctgccttca aacgctttga gcaggttttc caaccaaata atcgcgtcga tgtccaaatg
     8101 gttggtcggt tcatcgagca gcaatacgtc gggcttctgc acccaagcct gcgccaaggc
     8161 gacgcgcttt ttctgaccgc cggagaggtt gccgattttt tcgttttccg gcaaaccgag
     8221 ttcgcccaaa gtctgcttca ccgccgcatc caacttccag ccgtccttcg cttcgatctc
     8281 aagttgcaat tcgttgagct ctttcaataa ggcctcactc gaaccatttt ccaactcatg
     8341 gctgacatga tgataacggc gcaataaatc gcgaatttcg cccaaacctt cggcaacagt
     8401 atcaaatacg gttgcttcct tatcaaaaaa ggattcctgc ggtacataaa cgattttgag
     8461 gttgttttgg acaataatct gcccatcatc aagcttttgc acgccggcga ggatttttaa
     8521 aaacgaagac ttacccgcac cattacgacc gattaagccg attttttcgc ctctgtcgag
     8581 ttggaaggaa gttttgtcga gcagggcgac gtggccgacg gcaaaggaag cgttttctac
     8641 agataatata ttcatgatac aaattctcaa cagttaccgt ttggatttta ccgcaagttt
     8701 ggcgcgggca atttcaaccg cacccggcag gacggaaaca ataatgatgc cgcccatcac
     8761 caagcccaga ttgtttttta cgacggggaa gttggcaaag aaatagcccg cgtaagaaaa
     8821 caggataacc cacaacaagc caccgatgat gttgtagcgg ataaatttgg catagtgcat
     8881 tttccccata ccggcgacga agggggcgaa ggtgcggacg atgggcataa aacgggcaat
     8941 gatgatggtt ttgccgccgt gtttttcgta aaaacggtgg gttttatcga gatattcacg
     9001 tcggaagatt ttagaatcgg gattggcgaa cagcctgccg ccgaaatatt tgccgacggt
     9061 aaaattgagc gcgtcgccga gtatggcggc aaggcttaat aatgcaacca tcaaatgaat
     9121 atccataccg cccagcgcgg caataccgcc ggcggcaaac agcagcgaat cgccgggcag
     9181 taagggcgta acaatcaggc cggtttcgca aaaaacaatc aaaaacagaa tcgcataaat
     9241 ccacacaccg tattgcgccg acagcgcgag caggtgttgg tcgatatgga ggatgaagtc
     9301 gatggcggag gcaagcacgg cgcgttccta aaaaaaacaa accgcgtatt ttaaccgatt
     9361 ggaaaaatgc cgtctgaaat tttcagacgg catcggctat tcaaattcat ttcacgtaaa
     9421 aaccgcaaac caaaatagtt tgcggtttgg catttaaagt gacaatgatg atttcaaatc
     9481 atcagaattt tatgccgacg cgcaagccgt attcacgaat actggttttc gggatggtga
     9541 gcgatacgtc gccactcttg gttgttacac taaactcgcc ggattctttg taagtgcgtt
     9601 gtttgtagaa cggccccgcc tcgatgctgg cggattcgcc cagtttttta ccaatatttg
     9661 cacccaatcc aaagccccag ccgttggttt tttgattgat gtcttttttg agattggtaa
     9721 cgccggtgtt taatttatag cgggaattga ggtcaaattt cacttcagac caagggttga
     9781 tataccagcc gttacccagt tgggaaagca aatccgcgtg aactttggct aaccacgact
     9841 gacggctgct gtgaagcgta tgcttggtgg ttttaatgct gtcttttgaa gattcaaaac
     9901 ccaagccggc acccacacgg aaatttaaag aatcacttaa cgtttgggtg taggtgtagc
     9961 ctgtgtaaag atcgatacgg ttttcaggaa cgccggtggg cagttttaca tttttagtct
    10021 tacccagctt gttctcatct gtttccaaat taataatatt ttttttgctg cgcccgaaac
    10081 cggcttccaa gcggatgcct tggttggcat caaaaggaat atcagcacgt acgccgatgt
    10141 gtttggcagc tttgtgtttt tctttcagga aagcacgagt tgaagaaatg gaagagaggt
    10201 cggtgtggac ggtaaactca ttagcggttt gaagctcttg tgcagcggcg gcagtaccgg
    10261 tcagggcaat catggcacat gtaaaaactg tttttttcat agttaaaatc tccaaaattt
    10321 ggattgtagt cggatatggt aacataacgt aaataatcgt tacgcttaca attatattct
    10381 taagctttcg gggggggggg attttacgta tattaataaa aattaacaaa tagttatttg
    10441 tttacaacga attgttattc tcacttggtt ttctgttttt tatgggaatg acgaaatttt
    10501 aggtttctga tttcggtttt ctgttttaag ggaatgacga gacttgagat ggcggcattt
    10561 atcgggagca actgaaacca ctctgccgtc attcccgcga aagcgggaat ctaggttcgt
    10621 ccggtttcgg ttatttccga tagattcctg ccgcgttggg ggtctggatt cccgcctgcg
    10681 cgggaatgac gaatttcagg ttgctgtttt tggttttctg tttttgtgaa aataacagga
    10741 tgagatcttt gcaaaattcc tttccctccc gacagccgaa acccaaacac aggttttcgt
    10801 ctattttcgc cccaaatacc tcctaatttt acccaaatac ccccttaacc ctccccggat
    10861 acccgataat caggcatccg gtctcctttt aggcggcagc gggcgcactt agcttgttgg
    10921 cggctttcaa caggttcaaa cacatcgcct tcagatggct ttgcgcactc actttaatca
    10981 gtccgaaata ggctgcccgc gc
//
LOCUS       DAWFLT010000062        10949 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWFLT010000062 DAWFLT010000000
VERSION     DAWFLT010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10949
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.062"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..104)
                     /locus_tag="WGR74_001580"
     CDS             complement(<1..104)
                     /locus_tag="WGR74_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0905965.1"
                     /translation="MSTFFRQTAQAMIAKHINRFPLLKLDQVIDWQPI"
     gene            291..473
                     /locus_tag="WGR74_001581"
     CDS             291..473
                     /locus_tag="WGR74_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905966.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNVGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
     gene            complement(535..1536)
                     /locus_tag="WGR74_001582"
     CDS             complement(535..1536)
                     /locus_tag="WGR74_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ0905967.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGGVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(1613..5446)
                     /locus_tag="WGR74_001583"
     CDS             complement(1613..5446)
                     /locus_tag="WGR74_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ0905968.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETIQKRHQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAVPLDANSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSNGLNIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKHTRTVCMEFFGTVATATPSIVEIRDFLLAHESVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDETAVQTAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKERWDWLLANLNAPLADYKARYGAS
                     VHAAPEAKDNESCFTAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSNLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     AMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLQIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVQGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYTDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5707..5803
                     /gene="ffs"
                     /locus_tag="WGR74_001584"
     ncRNA           5707..5803
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WGR74_001584"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5970..7661
                     /gene="dld"
                     /locus_tag="WGR74_001585"
     CDS             5970..7661
                     /gene="dld"
                     /locus_tag="WGR74_001585"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ0905969.1"
                     /translation="MSASQLLSRLTQTVGEKYIITAPAKTEQYRQGYRFGEGRALAVV
                     RPGTILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKIGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPSEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            7793..7900
                     /locus_tag="WGR74_001586"
                     /pseudo
     CDS             7793..7900
                     /locus_tag="WGR74_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7956..8327
                     /locus_tag="WGR74_001587"
     CDS             7956..8327
                     /locus_tag="WGR74_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ0905970.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            8482..9030
                     /locus_tag="WGR74_001588"
     CDS             8482..9030
                     /locus_tag="WGR74_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0905971.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            9165..10256
                     /locus_tag="WGR74_001589"
     CDS             9165..10256
                     /locus_tag="WGR74_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0905972.1"
                     /translation="MNAQTLIANVAEFVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRAVKDK
                     YLADILQGKVLAGTGMSNTVKHLADIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNRYLDDGYDSLKTRLD
                     GAWAETERLAGLAWSGTPDNLTTLKLREAAAVLTLAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            10714..10884
                     /locus_tag="WGR74_001590"
     CDS             10714..10884
                     /locus_tag="WGR74_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ0905973.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIAPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT         2527 a         2986 c         2980 g         2456 t
ORIGIN      
        1 tcgatcggct gccaatcaat cacctggtcc aacttcaata gcgggaagcg gttgatgtgt
       61 ttggcaatca tggcttgggc ggtttgccgg aagaaggtgc tcatgagaaa tctcctaaat
      121 gtcttggtgg gaatttaggg gattttgggg gattttgcaa aggtctctat ttgtactgtc
      181 ttcggctcgc cgccttgtcc ttatttaaat ttaattcact atatttgccc gtgcgtttgt
      241 gataaattcg gcactgtatt tgatatgatt ttaacaaatc gggaggggca atgttttggt
      301 atgtaatcgg cttttgctcc tttgtcgtcg cgctgctgtc gctgtgggtc aatgtcggcg
      361 cattcggtat gcaggaagac gataccccgc aatcggatta tgaaaaacgt ttgggcttgg
      421 gtgcgaagct gaaaaacaaa aatacgccgg atacggaaga aaagcagcaa taaagatatg
      481 aaatgccgtc tgaaagattt tcagacggca tttgctttga agcatgggag acagtcaaac
      541 atttatctca tgctcaaatg cgttcaggat tcggcggact tcatccgcat ccttaaccgt
      601 tcggacgcgg tcaaacagcg tttgcgccgg gtcgaacgtt tttttcatca tgcccaacca
      661 ttgcttaaga cgggcgatcg ggtatttatt gtttgcctct tttgtcaggc acagctcgaa
      721 aaactgccgt atccatttag aaacttcggc aaaatccgtg tctttgaccg gcccgccgtt
      781 ctcgtattgc ttgatttgcc gcgccaaatc ggggcggatg accccgccgc gaccgagcat
      841 cacgctgttg cagccgctga ttgttttgat gccgatatag tcttgcaggc tgaaaacgtc
      901 gccgttggcg gtaacgggaa tattgacgct gtctcggatt ttccttatcc attcccaatg
      961 cgccggcggt tcgtaaccct cggctttggt acgcgcgtgt acggtcagtc cgcatgcgcc
     1021 cccttcggca atcgcacagg cgcattccaa agcccggctt ttgtcttcgt aaccgagccg
     1081 catttttgcg gtgagcggaa tatgtgcggg caaacgtccg cgcagcgttt tgacgatgtg
     1141 gaatatcagt tccggctctt ttaaaaggat tgcgccgcct ttgtgtttgt tgacggtggg
     1201 cgcggggcag ccgaagttca aatcgatttt gttcgcaccg aagcggacgg cttccagcgc
     1261 attcgccgcc atattgtccg catcgctgcc caaaagttgg acggtgcagg gcgtgccgga
     1321 aaacgttttg tttccgttgg cgatttcggg gacatatttt aaccatatgg atcgggaatg
     1381 cacggtatgg gtaatgcgta caaattcgct gacgcattcg tcgtagccgc caatacgcgt
     1441 cagcaggtcg cgcatcacgt catcgaccag accctgcata ggggcaagga tgattcgggt
     1501 tttttgtttg ggttcggttg tttgtccgtc aatcatgatt cgggtaggta tttgattcaa
     1561 acaatgccgt ctgaaggctt tcggcgttca gacggcattt ccgtgtccgt tgttacagca
     1621 atactttttc cacgccgccg ttgttggctt ttttcacaaa ctcgtctagc cagttttcgc
     1681 cgaggatgtg tttcgccatt tcgatgacga tgtagtcggc aggcatattg ttgtcgtcgg
     1741 tgtagcggct caagccttgc aggcaggctg ggcaggaagt aagcattttg acgggttcac
     1801 cctgcggcag ctctttgagg tttttctcga tttcctcttg tttgcggaat ttgacttggg
     1861 tggcgatatc gggtcgtttg acggcgaaca taccggactc gccgcagcag cggtcgctta
     1921 agacgacttt ctgccccatc aggctgctgg ccatttgggt ggcgttcatg gttttgatgg
     1981 gggtgtggca ggggtcgtgg tagaggtatt gctgaccttg cacgccgtcg agtttcacgc
     2041 ctttttcgag caggtattcg tggatgtcga tgatgcggca gccggggaag atttcttcaa
     2101 agcggtattt ttcgagctgg tcgtaacaag tgccgcaact gacaacgacg gtcttaatgt
     2161 cgaggtagtt gagggtgttt gccatacggt ggaaagccac gcggttgttg gtgctcattt
     2221 cttcggcttt agccttgttg ccgcccgcgt cttgcggata gccgcaacac atatagccgg
     2281 gcggcaggac ggtttgtacg ccgacgtgcc agagcatggc ttgaacggca agtccgattt
     2341 ggctgaacag gcgttccgaa ccgcaacccg ggaagtagaa cacggcttcg gcatcttcgg
     2401 gcgcggcggg gttgcggatg atggggatgc ttttgccgtc ttcgatgccc aataaggagc
     2461 gcggtgtttt ggcgggtacg cttttcggca aaggacggtt gatgaaatgg ataacctgtt
     2521 ctttaattgg ggctttgccg acggttgcct tgggttcggc tttttgcttt ttcgtgccga
     2581 tctgaagaag cttgccgatt ttgtaggcaa agttttgcgc tgggaagccg gtctgtatca
     2641 tggcggcgcg aagggctttg atggttttcg ggccggtggc gttcaaaaac gccataccca
     2701 tagctgcggc aggcgcaaat cgtttgtggc cggaatcggc aaggtagttg cggacggcta
     2761 cggttacgtc gccgaagtcg atgttgacgg ggcagggttt gacgcagcgg tggcacacgg
     2821 tgcagtggtc gccgatgtcc atgagttctt caaagtgttt gacggaaacg ccgcggcggg
     2881 tttgttcttc gtataagaag gcttcggtca gcaagcccac gccgaggatt ttgttgcgcg
     2941 gactgtacag caggttggca cgcggaacgt gagtagagca gacgggtttg catttgccgc
     3001 agcgcaggca gtctttgacg gaatcggcaa tcgtgccgag gtttgatttt tccataatca
     3061 gcgattccgc gcccaacagc tcgaaggacg gcgtgtaggc gttgcgtaag tccgagcctt
     3121 tcatcagttt gtggcggttg aaggtgtgtt tggggtcgac ttggtttttg tagtcccaaa
     3181 acggctgcat ttcttcgtcg cttaaaaatt cgagcttggt gatgccgatg ccgtgttcgc
     3241 ccgaaatcac gccgccgagc gaacgggcga ttttcataat gcgttcgact gagcggtatg
     3301 ccgtctgaag catttcggca tcgtctgagt taaccggaat attggtgtga acgttaccgt
     3361 cgccggcgtg catatgcagg gcgacaaaga cgcggctgcg tacggttttg gcgtggattt
     3421 ttcccaagcc ttggataatt ttggtgtcgg ttttgccgct gaagatttca gaaagcggct
     3481 tcattacgtc cgctttgaca gacacgcgca ggcggaaatc gcggaatgcg gtaaagcagc
     3541 tttcattgtc tttggcttcg ggcgcggcgt ggacggatgc gccatagcgg gctttgtagt
     3601 cggcaagcgg cgcgttcaaa ttggcgagca accagtccca acgttctttg acggcggaaa
     3661 cgtgggcaag ggcgtgtttg ccgcgttctc ccaaaagttc ggcggtcggc aggtccgtac
     3721 ccattttgtc gatggggagt ttgcccgaca gatattgctc caaggcggcg cagagtttga
     3781 gtttgttttg gatagaaagc tcgatgttga tgcgttcgat gccgtctgaa tactcgccca
     3841 gccgttccag cggaatcacc acgtcttcgt tgattttaaa ggcgttggtg tgtttggcga
     3901 tggcggcggt gcggctgcgg tcgagccaga aggttttacg cgcttcgggc gacacggcga
     3961 taaagccttc gccgtcgcgt gcgcgggcga gttcgcagat gtgttcggcg gctgtctgta
     4021 cggcggtttc gtcgtcggaa accacgtccg ccagcaagac cattttcggc cgccccttgc
     4081 ctgccgcttt ggtggcgtag ccgacagcgc ggacataacg ccagtccaaa tgctccaaac
     4141 cggccagtcg tacgctttca tgggcaagca aaaagtcgcg gatttcgaca atagatggcg
     4201 tggcggtggc aaccgtgccg aaaaactcca tacacacggt gcgcgtgtgt ttcggcattt
     4261 tgtgcaatac gaaggcaacg ctggtgatga tgccgtccgt gccttctttc tgcacgccgg
     4321 gcagtccgct caagaatttg tcggtaacgt ctttgcccaa accgactttg cggaacttgt
     4381 ggccggggat ttccaagcgt tcggttttaa cgatattgag gccgtttgaa tccagcgtgt
     4441 gaacgtcgaa cacggcggtt tcttcatcgt ggattttgcc gaaattgtgg cgcacgcgct
     4501 cgatgcgcag ccattcgcct tgagggttga ccatctgcca gtaggcgagg ttgtccaacg
     4561 ccgtccccca cagcacggct tttttgccgc ccgcgttcat cgccacattg ccgcccacgc
     4621 aggacgcgtc ggcggaagtc ggatcgacgg cgaacaccaa acctgcctga tgcgcggttt
     4681 cttccactcg tcgggttact acgcccgctc cgcaccggat aatcggatgc ttgccgtcca
     4741 agcccgccag ctcaacgtat tcaacaccac gatgcttgtc gagtttttcg gtattgatga
     4801 ccgcgctgtt cgcgtccaaa ggtaccgcgc cgccggtata acccgtgccg ccgccgcgcg
     4861 ggataataac caagtccaat tcgattaagg cgcgcaccaa aggcgcgatt tccgcctccg
     4921 tgtcgggatt gacgacgaca aaaggatatt cgacgcgcca gtcggtcgca tcggtaacgt
     4981 gcgttacgcg cgccaatccg tcaaacatga tgttgtgcgg cttggtaatt ttgcttaagc
     5041 gttccaaaat ttgatggcgt ttttggatgg tttcatcaaa actgctatca aaacgctcca
     5101 ctgctttttc tgctgcggca accaacacat cgacttgccg attatcgtcg cggcgtttgc
     5161 ggatttcgtt caacctgtgg cgcatttcgc gtaccagcgc ggcgcggcgt ttcgggtgct
     5221 ccagcaaatc atcgaccaga tacggattgc gcacgaccac ccaaatatcg cccaatactt
     5281 caaacagcat tcgtgccgaa cgcccggttt tacgctgtcc gcgcaggtct tgcaggattt
     5341 gccacgcttc gtcgcccaat aatcggatga cgatttcgcg gtcggtgtag gatgtatagt
     5401 tgtaggggat ttcccgaata cgctgcgggg cggtagtcgt ggtcatggtg tgtccgtgtt
     5461 tggcagtttt tatttgaatg ccgtctgaaa agtcggtttc aggcgggttt gaaaaggcga
     5521 taatgtagtt caatttttga tatttttcaa tgccgaaaca ggaaataccc ttatagaaac
     5581 aaaataacgc tttgagaaat tgttgacaat attatcaaat ataaatttta ggtggtgttt
     5641 ccggcgtatg accgtatcaa aacggttgag tttttcagaa aacccgttat aatgccgttt
     5701 ctctgaggcg ggtctccccg catggcaaat cggaacaccg ggtcaggggc ggaagccagc
     5761 agcccactcc ggtgcgccag tgccgggggt ttggcccgcc gccctatttg aaacgccgga
     5821 gcttcgatgt tccggcgttc ccgtatcgca ccgatgccgt ctgaaagccg ttcagacggc
     5881 attttcttta ctttgccata acgtataact acaatgtgaa aacaattcca atgtgcaatg
     5941 cacctgccga caattttaag gaaactgcca tgagcgcatc acaattactg tcaagactga
     6001 cccaaaccgt aggcgaaaaa tacatcatca ccgcccccgc gaagaccgaa caataccgcc
     6061 aaggctaccg cttcggcgag ggcagggcgt tggcggtggt gcgccccgga accattctgg
     6121 aaatgtggaa aattctgcag gcctgcgtcg aagcggacgt gattgtgatt acgcaggcgg
     6181 cgaataccgg tttgacgggc ggctcgaccc ccgacggcaa cgattacgac cgcgacatcg
     6241 tgattgtgaa caccatgcgg atgaacatca tccaaaccat caacaacaac gaacaagtcg
     6301 tctgcctgcc cggctcgacc ctgaaccagc ttgaattgct gctgaaacct ttggggcgcg
     6361 aaccgcattc ggttatcggc tcttcctgca tcggtgcgtc cgtcttgggc ggcgtgtgca
     6421 acaactccgg cggcgcattg gtgcagcgcg gccctgccta caccgaaatg gcactgttcg
     6481 cccaaatcaa cgaagaaggc aggttggaac tggtcaacca cttgggcata gacttgggcg
     6541 acacgcccga agaaatcctg accaacctgc aaggtcatca ttatcaaaac aaagacatca
     6601 aacaagacgc gggcaaaggg cacgaccacg cctattgcga acacgtccgc caagtggacg
     6661 aaccgaccgc cgcgcgtttc aatgccgacc ccgcccgcca ttacgaagcc tcgggctgcg
     6721 cgggcaaact gatggttttt gccgtccgtt tggacacctt cccgcaagaa aaacaaaccg
     6781 ccgtgttcta catcggcacg aacgacatca acgagctgac cgacatccgc cgcgccgcct
     6841 tgggcgaatt tgaaagcctg cctgtttccg gcgaatacat ccaccgccac gctttcgaca
     6901 ttgccgacgt gtacggcaaa gacacgtttt acgtcatcaa aaaattcggt acgcaccaac
     6961 tgccgaaatt atttgacttg aaggcgcgcg tggacaggtt cggcaaaaaa gccagcttcc
     7021 tgcccaaaca tttttccgac aaggccatgc agttcgtcag caaatacctg cccgaccacc
     7081 tgcccaaatc catgcgcgat taccgcgaca aatacgaaca ccacctgatt ctgaaaatag
     7141 gcgggaaagg cgtggatgag gcgcgcgcgt tcttaaaaga atattttgca caccacagcg
     7201 gcgcgttttt cgagtgcaac gccgaagaaa cccaagccgc gatgctgcac cgtttcgccg
     7261 tcgcctccgc cgccatccgc taccgcgccg tgcatgatga cgaagtggaa gacttggtcg
     7321 cgctcgacat cgccctgcgc cgcgacgatc gcgactggtt tgaaaaactg ccgtcggaaa
     7381 tcgacaataa aatcatccat aaactctact atggacattt catgtgccac gttttccatc
     7441 aggattacat catcaaaaaa ggcaacgact gcatggcgtt ggaacacgaa atgctgcatc
     7501 ttttagacca acgcggcgcg caatatcccg ccgaacacaa cgtcggtcat ttgtatgaag
     7561 ccaaacccgc gctcaaacag ttttaccgca aacttgaccc gaccaacagc ttcaacccgg
     7621 gcgtaggcaa aaccagcaaa aagaaaaact gggcggaata agcgcgtccg ctttgaaggc
     7681 aggcaatgcc gtctgaaggc aaaacgccgt tcagacggca ttgtcgggtt tgaagttccc
     7741 cgtcgggtaa tcgctttcaa accgtatgcc gacacacggg cgcgcccctc ccaaatatag
     7801 tggattaaat ttaaatcagg acaaggcaac gaagccgcag acagtacaga tagtacggca
     7861 aggcgaggta acgccgtact ggtttaaatt taatccacta taaagtctga tcctaccttc
     7921 ccaaagggca gggtttcaac cgaaaaggaa acacgatgaa accatacaaa atctacaccc
     7981 atcccgccct gccgccccaa gccgtcaaac aaggctggtc atggccgggg ctcttgttcg
     8041 gcacgctgtg ggcgtgtttc aaaagaatgt ggggtttggg gctggggctg acgggcgcga
     8101 tattcgtgct ggctgtgttt gcccaactgg tttacggcga cacgcccgcc acagactcgg
     8161 cgtttaacgt attgggcttg gcggtttccg tctggttcgg cgcaaagggc aacagccttt
     8221 atgcacgcca ccttttatcg cgcggctata ccgaattgcc cgaaacggtt gaggcagcca
     8281 atccccaagc cgcattggcg caatatttcg ggcgcggggg ccgataagcg cgttttcttt
     8341 tccatcgtgc cgtctgaaaa atgttcagac ggcatttctt ataacctttc gtttgcagca
     8401 cataaccaat cagtctttcc tattccgttc cgaaatgcat accatgcgcc cgtacccaat
     8461 atttgcgaac aaggaaagaa aatggcacgt ttaaccgtac acaccctcga aaccgccccc
     8521 gaagccgcca aagcgcgcgt cgaggcggta cttcaaaaca acggctttat ccccaacctt
     8581 atcggcgtat tatcaaacgc ccccgaagcc ttggcgtttt accaagaagt cggcaagctc
     8641 aacgccgcca acagcctgac cgccggcgaa gtcgaagtaa tccagattat tgccgcccgc
     8701 accaaccaat gcggcttctg cgtggcaggg cacaccaaac tcgcaaccct gaaaaaactc
     8761 ctttccgaac aatccgtcaa agccgcgcgc gctttggcgg caggcgaatt tgacgatgct
     8821 aaactcggcg cgctcgccgc ctttacccaa gccgtaatgg caaaaaaagg cgcggtatcc
     8881 gacgaggaac tcaaagcatt ttttgatgcg ggctacaacc agcagcaggc agtcgaagtc
     8941 gtgatgggcg tagccttggc aactttgtgc aactacgtca acaacctcgg acaaaccgaa
     9001 atcaaccccg aattgcaggc ttacgcctga tacggcaaac cgcccgaata tcgggcggtt
     9061 tttcaaaaac accccctcaa acaaaaacaa gccgcctaag cggcagggaa gcagccgcgc
     9121 atcaggcgcg cccccgattt ccccgcaaac cttaaggaac aaagatgaac gcccaaaccc
     9181 tgattgccaa cgttgccgaa ttcgtcaaaa ccaagctcaa acccatagtg gacgacatcg
     9241 accgcaaagg atactacccc gaagcattta tgcgcgaact cggcgcaatc ggtggattcg
     9301 gcgcagtcgg tacagaagcc gaaggcggca acggcttggg tttggcaacg caaatcgccg
     9361 tattgcgcga aatcggcaaa gaatgcggcg caacctcttt cagtgcgtgg tgtcaggcgg
     9421 cttgcgcgtg gtatctgcac caaacgccca accgggccgt caaagacaaa taccttgccg
     9481 acatcctgca aggcaaagta ttggcgggta cgggcatgtc caataccgtc aaacaccttg
     9541 ccgacatcga aaaacacaac ctccaagccg agcgcgtgga gggcggctac acagtcaacg
     9601 gcgcgctgcc gtgggtgtcc aacatcggcg aagaccacat ttgggcgaat accgcccaaa
     9661 tcggcggcgg ctacgtcatg ttcatcacag gcggacaatg ggaaggcgta agcctgcaaa
     9721 actgccccga attttgcgcc ctcgaaggca cacgcacctt cagcctgaac ttcaaagacg
     9781 tatttatccc cgacgaagac atcatcgccg cgcccgaaca gtttgccgac tacatccaaa
     9841 gcatcaaagc aggctttatc ctcctgcaaa tcggcatcgg cgcgggcgtg atagacggca
     9901 gcctcggcat catccgcctc gccaacgtcg tcaacgccga agtcaaccgc tatctcgacg
     9961 acggctacga cagcctcaaa acaaggctgg acggcgcgtg ggcagaaacc gaacggctcg
    10021 ccggattggc ttggagcggc acgcccgaca accttaccac cctcaagctg cgcgaagccg
    10081 ccgccgtact gacacttgcc gccgcccaat ccgccgccct gcattccggc gcaaaaggct
    10141 acctgatgcg gagtcccgcc caaaggcgcg tccgcgaagc aatgtttgtc gccatcgtaa
    10201 ctcccgcgat caaacacctc cgcaaagaaa tagcggcaat cgaagccgca aaataaacag
    10261 gcggaaaaaa ccgaaacccc catcgcgtca ttcccgcgaa agcgggaatc cagcccccaa
    10321 cgtaacagga atccatcgga aaaaacagaa accctcaaac cgtcattccc gcaaaagcgg
    10381 gaatccagcc ccccctaacg caacaggaat tcatcgaaaa aaccgaaacc tccaaaccgt
    10441 cattcccacg gaagtgggaa tccagtaatc gaaaaaccac aggaatctat cgaaaaaaaa
    10501 ccaaccccaa aaaaaccggg cggataccgc accattctcc caaaccctga tttatagcgg
    10561 atgaacaaaa atcagggcaa ggcggcgaag ccgcagacag tacaaatagt acgggtgctt
    10621 cagtacttta gagaatcgtt ctcttcgagc taaggcgagg caacgccgta ccggtttttg
    10681 ttaacccact atacaaaaaa caaggaaaca caaatggcgc aatatatgtg cggcccctgc
    10741 ggctggattt acgatgaaga acacggcgac cccgaacacg gcatcgcccc cgggacaaag
    10801 tttgaagaca tccccgacga ctggaaatgc cccgaatgcg gcgtgggcaa agaagatttc
    10861 tacctgttgg atttcgtgat atagcggcag ccgcaccctt agggaaaacc ccgtcatccg
    10921 gcgttccgcc tgcggcggca gggaacacc
//
LOCUS       DAWFLT010000063        10727 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWFLT010000063 DAWFLT010000000
VERSION     DAWFLT010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.063"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(293..505)
                     /locus_tag="WGR74_001591"
     CDS             complement(293..505)
                     /locus_tag="WGR74_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0905974.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAKPD
                     TETRRTDMSLEKWRQFLDWTEKTNAV"
     gene            complement(517..798)
                     /locus_tag="WGR74_001592"
     CDS             complement(517..798)
                     /locus_tag="WGR74_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905975.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDSEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(836..1099)
                     /locus_tag="WGR74_001593"
     CDS             complement(836..1099)
                     /locus_tag="WGR74_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905976.1"
                     /translation="MADILPSDKRRQPPKTKGSTMNIIEQEMKHSSLRKVDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            complement(1127..1700)
                     /locus_tag="WGR74_001594"
                     /pseudo
     CDS             complement(1127..1700)
                     /locus_tag="WGR74_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1675..2019)
                     /locus_tag="WGR74_001595"
                     /pseudo
     CDS             complement(1675..2019)
                     /locus_tag="WGR74_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2012..2887)
                     /locus_tag="WGR74_001596"
     CDS             complement(2012..2887)
                     /locus_tag="WGR74_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ0905977.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELAEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHGISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2932..3222)
                     /locus_tag="WGR74_001597"
     CDS             complement(2932..3222)
                     /locus_tag="WGR74_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0905978.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3234..3413)
                     /locus_tag="WGR74_001598"
     CDS             complement(3234..3413)
                     /locus_tag="WGR74_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0905979.1"
                     /translation="MYGYTVEKLKENFAQNGQTLAQWARENGFKPRDVYLVVGGQRKG
                     NYGKGHEIAKKLGLK"
     gene            3527..3904
                     /locus_tag="WGR74_001599"
     CDS             3527..3904
                     /locus_tag="WGR74_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0905980.1"
                     /translation="MISKSLFGNRLKEKRKFLGLTQAQAAEKAGIERETWGKYERGVF
                     MPSGDVLLSFLNMGIDVSSLFAAEQGIRPSENSEISKEESELLGYYRQASDNGKFVIL
                     SVSKGAEKKAAENTQVSATHKTA"
     gene            3933..4178
                     /locus_tag="WGR74_001600"
     CDS             3933..4178
                     /locus_tag="WGR74_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905981.1"
                     /translation="MFDFEQQIKWGERAEEIVKEAATQNNIEIPEPLASALAKAVKVH
                     YLSQAGVFSLVEAYADTVNPTEKEVDYQAIGKELFEK"
     gene            4412..5380
                     /locus_tag="WGR74_001601"
     CDS             4412..5380
                     /locus_tag="WGR74_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905982.1"
                     /translation="MAKKTTKTIHYLRAQQNHSLFDLEVALRRVLSAAPTVQDTQIEQ
                     YNQLTQIMHRTLDPKDVKGNSIGGLLIHIGSGTKDEHIRTMSNRPVQQDDHGGTQAPP
                     SGYSFLRKEAFLYIVGHHVIFCGHGFLSASTVASYLSLLSNKLRENNPDIVQFNIEFK
                     AVGNCDKLSLIQQHGVKSILLDASAYQLSRNRLYQDSRSTIAKALGKVGSVFTSELSD
                     EELEAQSEIHINLEVLLNGNSRASIEAQSLMQEQAEEIIDDETVNQGFSITTQQGEVI
                     QPSDVKLSKSVRIGRYDEANSLLPDSAFTAISEYFLELQSRNLTEQ"
     gene            5377..5859
                     /locus_tag="WGR74_001602"
     CDS             5377..5859
                     /locus_tag="WGR74_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905983.1"
                     /translation="MKKYLPLLRFLLIACISAFLAWKGQPFVHGNEKAVDLIINVFAI
                     LAGFLIAIMTLFSDMRFDEDANWRQIQIREGVQEQRYIKHSLLFYTYLAVLVCVFIVI
                     LLAHKEEYKNGPAIFWLERSYLFLACISIFYSVFLPGNLIKSRKEEFKKLMEKKKPKI
                     "
     gene            5949..6452
                     /locus_tag="WGR74_001603"
     CDS             5949..6452
                     /locus_tag="WGR74_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ0905984.1"
                     /translation="MSDEFNRFIERVLSHEGGYVNHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAIGIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     AAADNAD"
     gene            6561..6893
                     /locus_tag="WGR74_001604"
     CDS             6561..6893
                     /locus_tag="WGR74_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905985.1"
                     /translation="MLDASPPQERGSRRETRSQKKASQITAFFRWLGGLVSNPATGKI
                     SHTKLWANVAAAAMTWKFVQAADAPEWLWWAYGAMVGGYALIKRGIAVVPQLAEVKKS
                     ANQEGGRQ"
     gene            6890..7300
                     /locus_tag="WGR74_001605"
     CDS             6890..7300
                     /locus_tag="WGR74_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ0905986.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMVALKKPDALIHGKEVRQKERNSSQG"
     gene            7315..8037
                     /locus_tag="WGR74_001606"
     CDS             7315..8037
                     /locus_tag="WGR74_001606"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ0905987.1"
                     /translation="MNCCWGLSFYYQLAVQSAEDKYADLKRHIHDIYRRHKGRYGYRR
                     IAAAIRHAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNILRRCFHAEK
                     PNEKWVTDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQIRPTFDLAGEILKGAPEKPR
                     SSEKPILHSDQGWQYQMFFYSGLNLNQDKAMKPQTVQIVRQGEATPYWFKFNPLYNND
                     RIKLKLKGLSLVQYRIQSLKAA"
     gene            complement(8358..8720)
                     /gene="ssrA"
                     /locus_tag="WGR74_001607"
     tmRNA           complement(8358..8720)
                     /gene="ssrA"
                     /locus_tag="WGR74_001607"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8783..9388)
                     /locus_tag="WGR74_001608"
     CDS             complement(8783..9388)
                     /locus_tag="WGR74_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ0905988.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
     gene            complement(9866..10051)
                     /locus_tag="WGR74_001609"
                     /pseudo
     CDS             complement(9866..10051)
                     /locus_tag="WGR74_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(10139..10727)
                     /locus_tag="WGR74_001610"
                     /pseudo
     CDS             complement(10139..10727)
                     /locus_tag="WGR74_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216327.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2558 a         2494 c         2728 g         2947 t
ORIGIN      
        1 gcgcccgctg ccgcctaaaa ggcgaccgga tgcctgatta tcgggtatcc ggggaggatt
       61 aagggggtat ttgggtaaaa ttaggcggta tttggggcga aaatagacga aaacctgtgt
      121 tggggtttcg gctgtcggga gggaaaggaa ttttgcaaag gtctcagagt gagtttattt
      181 tggggcggcg gcgggtcggg ggcaagcggc gtggggcttg gttgtggttt ttaggtttgg
      241 gggtgggtaa aaaatgccgt acccggcggg caggcttcgc actctgaagg tttcagacgg
      301 cgtttgtttt ttctgtccaa tcgaggaact gccgccattt ttccagcgac atatcggtcc
      361 gccgcgtttc ggtatcgggt ttggcttccc agcggcctac ctgtatgtag tgcttcaccc
      421 cgacgatttg cgccaactcg gcctgtgtca gtttgcagcg gttgcgcagg gtgcgaaggt
      481 tgtaaggcgt gtagccgagt tccatgtcgt tgcggtttat tttgtttcgc atattttttt
      541 gactgcccgg cggcaggttt cggtaaggac ggcggcaaat cgggcttcgt ctgccggttt
      601 tccgtcgaaa aaaataaaat cgtaaagggt gatgccgttt tcggaatggc ggtaatgccc
      661 gcccgggcat tcgctgtctg tctgcctgtg gattttggca atcaggcggg gatagcggca
      721 atgggtaatg aaacggcttg cgccgtcttg cccgataatc cattcggggt agcggttgaa
      781 tagtgcggct ctgttcattt tgttcggggg ataaagcccc tcgcggggct tgtggtcagg
      841 caaatttgaa tatcagtgcc aacacggcgg cgatggcggt aaccagcccg gtggccgcca
      901 tcatgggata ccatcgggat tcttgggcta tttttacgga ttcggcgttg attttgtgcg
      961 cgtctgcaat gattttggcg atttcggcat ctacttttct cagactggag tgtttcattt
     1021 cttgttcgat tatgttcatc gtacttcctt tcgtttttgg cggttgccgc cgcttgtcgg
     1081 atggtaggat gtctgccatg tgtatatatt tatatattga taccttttag gttttattgc
     1141 aagtgttttg ggcggcggct tcgtatgctt ggcggtggcg gcggctgtac cattcggcga
     1201 gttcgcgccg ctcttggagg cggtagctgt cggcggtcag gtaatggtgc aggcttgaga
     1261 agccggcgcg ttggagggcg gcgcggctga tgtggccgag ggcgaggtct gttgtgaggg
     1321 tgtcttttcc ggcggtcagg ctgatgttgg tgaagaggtg gcggaatccg tgcattgtgt
     1381 gtttggattt gccgggggtg ctgccgtcat agcccagtcg tcggatggcg ttgtgggcga
     1441 atttgatgct gatgtggtcg ggatgggggg gcgggtttgc gccgtgggcg gatgcagggg
     1501 aaaaggtgga tgttgtcgcc ggtctgtgtg tgcagctctc ggagtatttc taccgcccag
     1561 tccgacagtg ggacggtaaa ggggtgtttg gtcttcatgt cggcggcggg gatgtgccat
     1621 aaccgggcgg tgaggtcgat gtcttgccag cgggcggaaa gcagtgcgga cggacgggat
     1681 gacttccccg gtttccccat caatccgcgc cgcctcgatt tttccgttgg cgcgcagcat
     1741 ttccaccgcc tgccgcaccg acatcaaccg cttgccgttg cgcctcatcg cctccaccaa
     1801 aaccgccgaa atcaatttgg cttcttccgg tttaagctcc gtcttgcccg catcgctgcg
     1861 ccgtttgcgc gtcggcttga cgctgaccgc ctccagcttg cggtatagcg tggcaaggct
     1921 gatgcccaat tcctgcgcct gctgcttaag atatgcagag cgtgcgccgc gttccattgc
     1981 ttccgcctga ttctcgactg ccttaagacg ctcaatcatt gccggattca tcgccttctc
     2041 ccgtttcacc gcccaaccat tccggcacat tgtctgtcgg tgcttcagtc ggcagggcat
     2101 agctttcgcg cagttgctcg cagtccaaaa taatttgatt gagcgtgccg accatctttg
     2161 cctgatggct gatcccgtgt gcctcactgt gcgcattaag ttggtcgaac aaatctttca
     2221 gacggctcac ttgactgcgg ataccgacct caaggcttgt taactgcatc gccaactcgc
     2281 tgcctacgtc ttccgccttc ggctctctga caacggtttg cttcttagcc agcttctcgg
     2341 ccagctcatc aattttggct gttttggttt tcatcacttc gtctttggcg gcgaggtttt
     2401 cgcggctttc gcgcagggcg acgcgcagct cgcgcaccgt cattcggtcc acatcgtcaa
     2461 aggtcatgcc gttgacttct tccccttcgg ccaaacccac cagcgtaacg tcttcttcga
     2521 ccagcagctc aagcagcttc gacttgccca aatccatcag cttcggcgcg gctttctgca
     2581 tttgcggggt cgcaaagcgg cgagtggctg acatcagacg tgatgtttct gcgataccca
     2641 gcccaaattg gcttttcaca atttccataa accgtccgtg ttccgtatgc tcttttaaaa
     2701 taatcagcgc acgtcccagt tcgaacatgc cttccatcgt ctgccgtact gcttgacgac
     2761 cgcgttcaac ccaacgctct tcgctataag tctcgccgtt accccactgc tccatcacca
     2821 gcacactgcg catcgctgcc tgattgctta ctttgtcggt tgcgataatt tcaatttctg
     2881 tattcatttt ttatctccaa agtttccgac gtcggaaact ttcaaaatcc gttaatccac
     2941 atcgacccgc ttgccgattt cggcaatctt gctttgcagc cgttcatgct gctgcctgaa
     3001 ccgctctgcg atttgcaggg ttttgatgcc gtaggcgtag ttgccgtttt caagtttgat
     3061 gaccaatccc gaggcaacca aatcatcaat atccctgctg acttgcgatg gcgtcagccc
     3121 cagtccgacc gataaatcct tattgctcag accgataatc ggatgctcgt caagcgcgat
     3181 aaagaccctc aatagccgtt gtaccctttt actttctgcc atccgcatcc tccttatttc
     3241 agtcccagct tcttggcaat ttcatgcccc ttgccgtaat tgcctttgcg ctgtccgccg
     3301 accaccagat acacatcgcg cggcttaaag ccgttctctc ttgcccactg cgccagcgtt
     3361 tggccgtttt gggcaaaatt ttcttttagt ttttcaacag tatagccata gatagccctt
     3421 tcccattgtg ataaaatagt gaactattta tgcactagaa attaattagt gcattaccaa
     3481 tgcgtgaatt atgagatttt aaaatctctt ttgcaaggat tattttgtga tttctaaatc
     3541 tctttttggt aatcgtttga aagaaaaaag aaagttttta gggcttaccc aagcacaagc
     3601 tgctgaaaaa gcaggtattg agcgcgaaac atggggcaaa tacgaacgtg gcgtatttat
     3661 gcctagtgga gatgtattgc tctctttttt gaatatggga atagatgtga gcagcttatt
     3721 tgctgctgaa cagggaatta ggccgtctga aaattctgaa attagtaagg aagagtcaga
     3781 gttattaggg tattaccgcc aagccagcga taacggcaaa tttgtcattc tcagtgtgtc
     3841 aaaaggcgca gagaaaaaag cagccgaaaa tactcaagtt tcagccacgc ataaaacagc
     3901 gtgattttta aatccaaaat ggagacagtc ttatgtttga ctttgaacaa caaatcaaat
     3961 ggggtgagcg agcggaagaa atcgttaaag aggcagccac ccagaacaat atcgaaattc
     4021 ccgaaccttt ggcttcggca ttagctaagg cggtaaaagt gcattatcta agccaagctg
     4081 gtgtgttttc tttggtagaa gcctatgcag acacggtaaa tccaactgaa aaagaagtag
     4141 attaccaagc aatcggcaaa gagctatttg aaaaataaaa aatgccgtct gaaattttca
     4201 gacggcattt aggacactcc gacagtcggg ctgctccggt gttctgaaac ggatattaag
     4261 caattgaagc ggccgtgcat tttaaaacgc attaaaaact accccgacat acaggtcatc
     4321 gggcaggtgg ttcaggtgtt gaaagatttg aattgatgcc gtctgaattt attttttcga
     4381 cacagatatt aaaagagaaa ggactgtaca aatggcaaaa aaaactacta aaactattca
     4441 ttatttacgt gcgcagcaaa accatagctt gttcgattta gaggtagcat taagaagggt
     4501 tttgtcagct gctcctactg ttcaagatac acaaattgaa caatataatc agcttaccca
     4561 aatcatgcat agaactttgg atcctaaaga tgtaaaaggc aattcaattg gtggattact
     4621 gatacacatt ggcagcggta caaaagatga acatatccgc actatgagca atagacctgt
     4681 acagcaagat gatcatggtg gaacacaagc tcctccaagt ggatattcct ttttgcgtaa
     4741 agaggctttt ttgtatattg ttggacacca tgtaattttt tgcggacatg gttttttgtc
     4801 agcatcaacg gttgcttctt atttaagttt acttagcaat aaattgagag agaacaatcc
     4861 ggatattgtt caatttaata ttgaattcaa agcagtaggc aattgcgaca agttgtcatt
     4921 gatacaacaa catggggtaa aaagtattct tttagatgct tcggcatatc aattatccag
     4981 aaacagactg tatcaagata gccgttcaac cattgctaaa gcattaggga aagttggtag
     5041 tgtatttaca agcgaattga gtgatgagga attggaagca caatccgaaa ttcatatcaa
     5101 tttagaagtc ttgctaaacg gtaattcgag agccagtatt gaagcgcaat cgctgatgca
     5161 ggagcaggca gaggaaatta ttgatgatga aacggttaat caaggatttt ccattaccac
     5221 gcaacaaggt gaagtgattc agccctctga tgtaaaactg tctaaatctg ttagaatagg
     5281 acgatacgat gaagccaact cgttactccc tgactcggca tttacagcaa tcagtgaata
     5341 tttcctagaa ctccaaagca gaaacttaac tgaacaatga aaaaatatct gcccttacta
     5401 cgttttttgt tgattgcttg tatatcagca tttttagcat ggaaagggca accttttgtt
     5461 catgggaacg agaaggccgt tgatttaatt attaacgtat ttgcaatctt agccggtttt
     5521 ttaattgcca ttatgacatt gtttagtgat atgaggtttg atgaagatgc aaattggcga
     5581 caaattcaaa ttcgtgaagg cgtacaagaa caacggtata taaaacattc cttacttttt
     5641 tacacatatc ttgccgtatt agtttgtgtt tttattgtca tcttgttggc tcataaagaa
     5701 gaatacaaaa atggaccagc cattttttgg cttgaacgga gctatttatt tttagcctgt
     5761 atttccattt tttattctgt attcttacct gggaatttaa ttaaaagccg aaaagaagag
     5821 tttaaaaaat taatggaaaa gaagaaaccc aaaatctaga acgagtttta acccccttta
     5881 aaatcccatt cagacgacct tttctaaaat ccctgtattg atttttaatt caatacaggg
     5941 atttttccat gtcagacgaa ttcaaccgat ttatcgaacg cgtcctctcc cacgagggcg
     6001 gttacgtcaa tcatcccaaa gaccccggcg gcgaaaccaa ttggggcatc actaagcgca
     6061 ccgcacaggc aaacggctac aacggctcca tgcgcgccat gacgcgtgaa caggcaatcg
     6121 gcatttaccg taaagcgttt tgggagcgtt accgcgccga ccaaatgccc gaagcggtcg
     6181 cttttcaatt ttttgatgcc tgcgtcaacc acggttacgg caatgccgcc cgtatgctcc
     6241 aacgcgccgc aggcgtgccg gacgatggaa tcatcggcga aatcagcctc aaagccatca
     6301 attcccttcc cgaaaacgac cttttattgc ggttcaacgc cgagcgtctg gtcttttata
     6361 ccaagctggg tacgttcacc tctttcggca agggctgggt acgccgtgtg gcgcaaaacc
     6421 tgattcacgc cgccgcagac aatgccgatt gaggagcaaa ccatgtcaaa aaagtccatt
     6481 ctcgccctga tggccgcagc catgcagccc gatttcagcc acagcgacct aggcattcgc
     6541 tacgccatgc cgactcaggg atgttggacg caagcccacc gcaagagcgg ggtagccgcc
     6601 gcgaaacgcg cagccaaaaa aaagcgtcac aaataaccgc ctttttccga tggctgggcg
     6661 gcttggtctc taatccggcc acagggaaaa tcagccatac caaactttgg gcaaacgtcg
     6721 ccgcagccgc catgacttgg aagttcgtgc aggcggcgga cgcgcccgaa tggctttggt
     6781 gggcatacgg cgcgatggtc ggcgggtatg cattaatcaa acgcggcatc gccgtcgtac
     6841 cgcagttggc ggaagtcaaa aaatccgcaa atcaggaagg ggggcggcaa tgattgaatt
     6901 tgtccgagcc aaaaaacggc tgctttgggc atttgtgctt ttgcttgtgt ggacgtgcgg
     6961 ttaccgatac gccgccgaca aggccgaagc ggaacaaacc gccctgattg ccacctatcg
     7021 gcattcttct atggttgcgg cggaacaata tgccttgcag cttaaaaaag cgcaggacga
     7081 aaggcagcgg tggtacgact tttcccaaaa acaaggaaga aagcccgtga aaaaacagta
     7141 tccgccgcaa acgaaaaaag ccggctatct gaaaaccaag gaagaactgc ttgcggaatt
     7201 ggcttgcctt aaagcggaaa tggttgccct aaaaaagccc gatgccttaa tccatgggaa
     7261 agaagtgcgg cagaaagaac gcaactcgtc gcagggttaa ggcaatgcca tccgttgaac
     7321 tgctgttggg gattgtcctt ctattaccaa ttggccgtcc aatcggcaga agacaaatat
     7381 gccgatttga aacggcatat ccatgatatt tatcgacgac ataagggaag atacggctac
     7441 cggaggattg cggcagccat ccgtcacgca ggaacaccgg tcaatcacaa gaaagtcagc
     7501 cgtctgatgg cgaagacggg gctgaaggca gtgatacggc ggcgcaaata ccgctcgttc
     7561 aaaggagaag tcggcaaaat tgcgccgaat atcctgcgac gctgtttcca tgcagaaaag
     7621 ccgaatgaga aatgggtaac ggacgttacc gagttcaatg taggcggaga aaagatatac
     7681 ctttctccga ttatggattt gtttaacggg gaaatcgtca gttaccgtat tcaaatccgc
     7741 ccgactttcg atttggccgg cgagatactg aaaggtgcgc cggagaaacc gagatcgtct
     7801 gaaaagccga tactgcattc ggatcaaggt tggcaatatc agatgttttt ttatagtgga
     7861 ttaaatttaa atcaggacaa ggcgatgaag ccgcagacag tacaaatagt acggcaaggc
     7921 gaggcaacgc cgtactggtt taaatttaat ccactataca acaacgatag aatcaagttg
     7981 aaattaaaag ggctgagcct tgttcagtac agaattcagt ccctgaaagc cgcttgatta
     8041 aactgtccga ctttttgggg tcagttcggc ttcggcattt ttttatccgt ttgggggtaa
     8101 cttgtttgga aagctgcaag cttataaata aaggattaca tttaagtttt gggtaacctt
     8161 tttaaaaaaa tgcgtgatga cttttgcatt cttaaggcgt tttttggggt aattcgtgaa
     8221 aagagttacc ccaaaagtta ccccataaat ggcgaaaact caagcatacg ccagcatatt
     8281 caaggcaaca aaaaagcctt gaaactgttg aagttcaagg cttttttgtg tttcaggata
     8341 ctgctgaaaa tagggtatgg tggaggcggg gggaatcgaa cccccgtccg aaagtcctct
     8401 acaaagcgtt ctacatactt agttgtgtct atttgaaaat cttatttcca tcatgccgac
     8461 caacaggcct tttggaaacc agttacctta aatctcattt cctgccaagt aacccggcag
     8521 gaaaccagtc aatgtaagat gacgttgcgg tggctttcgc cacacagccc attgaccgac
     8581 tgctgcaacg gctagcctta agcggctaaa gcgtaagttt cgtcgtttgc gactatttga
     8641 attcagtgtt ttacgggaat ctgagacccc ggtatgcccg catctgcttc gcaacccccg
     8701 tcgaaaccaa ggtcgccccc aaaaatggtt tgcaaattat acggatattg tgcggtgctg
     8761 ccaagtctgt cggagaaatt tgtcagtctt gctgccttaa tttgcgtttg agcaggatgc
     8821 ggacgcagcc gtcgttgcct tcccggggtt cggcgtaggc gagtacatcg gggtgttgca
     8881 tcagccagtt tcgggtcata tttttcagaa cgggtttgta gcctttggaa cctaatccgc
     8941 tgccgtggat gatttcgccg catacgccgc gtttttgggt gaatgcgatg aattcgttga
     9001 ggactttttg ggcttcttcc tgtgtgtagc cgtgcaggtc gacatcggta acgacgggat
     9061 agtatccgtt tttcaggcgt tggatgtcgt tttttccctg tccgtttttg ctgaagctgg
     9121 cgggcgggtc gttgtatgtg ctgccgatgt agaagtagtt ttcttcgtcg gcgcggttgt
     9181 ctttgggacg gactttgatg ggggttttgt cgggcggcgc ataatattgc tgccggtttt
     9241 ttaatgggga gacttgtccg actgcttgtg aaaaatcgaa atcctgttct tgtttttgct
     9301 tgtttttttc ggctgcctgt ttttctgccg cttctttttg ggcttgtttg cccagttgtt
     9361 tgaggatgtt ttggaagtcg gtattcatat tttttcctgt tatttgtccg atggctgttt
     9421 cgggcggggt tttaatttgc cggaatgttt gccaatcggg ggaggatgat tttgttgccg
     9481 tcgtatgttt tttgaaagtg tgattgtata tcaaaaagaa atgcggcaac cgtcggcagt
     9541 gttgattgcc ggaaatgcgg gccggtcgaa ccgatatgcc cgaacgcctg ataaagtttt
     9601 aaaaacctgc cttgcgaagc aggttaatgg ttttgccaat tttgaattgc cggaaacgcg
     9661 aaacacggaa atctgatgtt ttataaatgt tccgatacga actgcaaaat attggttgag
     9721 acctttgcaa aattcctttc cctcacgaca accgaaaccc caacacaggt tttcggctgt
     9781 tttcgcccca aatatctcct aattttaccc aaataccccc ttaatcctcc ccggataccc
     9841 tataatcagg catccgggct gccttttagg cggcagcggg cgcacttaac ctgttggcgg
     9901 ctttcaacag gttcaaacac atcgccttca gatggctttg tgcactcact ttaatcagtc
     9961 cgaaataggc tgcccgcgca tagcggaatt tacggtgcag cgtaccgaag ctctgttcga
    10021 ccacataacg ggtcttcgat aaataccggt tgagaccttt gcaaaattcc tttccctccc
    10081 gacagccgaa acccaaacac aggttttcgg ctgttttcgc ctcaaatacc tcctgatttt
    10141 acccaaatac ccccttaatc ctccctggat acccgataat caggcatccg gtcgcctttt
    10201 gggcggcagc gggcgcactt agcctgttgg cggctttcaa caggttcaaa cacatcgcca
    10261 cgggtcttcg acaaataccg gtcgcgtttg gtttgcgttt ccgtcagcgg acggttgcgg
    10321 caggctttgc gcataatgcc gtcctgcaac tgatgttctt ccagatgttg ccggttttcc
    10381 gcactgtcat agcctttgtc ggcatagacg gtcgtacctt tgggcagtcc ttccaacaaa
    10441 ggcggcaggt gtttgcactc atgggcattg gcgggggtga tgtgcagttt ctcgatatag
    10501 ccttccacat cggtacgggt atgttgtttg taaccgagtt tgtagaggcc gtttttcttg
    10561 atccaacggg catcgctgtc cttactcggt gtggtttggc cgctgatttg tccttcctca
    10621 tcgacttcta tagcctgacg ctgtttgctg ccggcggtct gaataatggt ggcgtcaacg
    10681 acggcggcgg atgctttctc tacttttagg cctttttcgg tcagttg
//
LOCUS       DAWFLT010000064        10674 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWFLT010000064 DAWFLT010000000
VERSION     DAWFLT010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10674
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.064"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(63..1205)
                     /locus_tag="WGR74_001611"
     CDS             complement(63..1205)
                     /locus_tag="WGR74_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ0905989.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQAFDGGVSCRGGL
                     EQVYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKIEAKR
                     ANVKSLQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLNERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIATGRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQTRPVRAPIAGSDSDRRIVQTALDNARSA
                     EVDDIVSFGVADAQSVRPNGENGIMVSNPPYGVRLEEVHALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1254..1676)
                     /locus_tag="WGR74_001612"
     CDS             complement(1254..1676)
                     /locus_tag="WGR74_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0905990.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPVGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1669..2478)
                     /locus_tag="WGR74_001613"
     CDS             complement(1669..2478)
                     /locus_tag="WGR74_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ0905991.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWTGKPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMVVQTREQWRRAQEKGADAVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2560..2937)
                     /locus_tag="WGR74_001614"
     CDS             complement(2560..2937)
                     /locus_tag="WGR74_001614"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ0905992.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(3137..3904)
                     /locus_tag="WGR74_001615"
     CDS             complement(3137..3904)
                     /locus_tag="WGR74_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0905993.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFNMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSIFGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3914..4693)
                     /locus_tag="WGR74_001616"
     CDS             complement(3914..4693)
                     /locus_tag="WGR74_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ0905994.1"
                     /translation="MNQKYILSANNNSLIEEIHNTVQRIGYCIVRGLNLNHLDDSRRN
                     KKLFDFLSQLGMLTNHKDDGFKSIFWDIKYRGDDYVINNDITFSEDVGECPLHSDSSF
                     SENPESYLVMYVVKSANDGGNSLFLSSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPT
                     SFDKKQGVRWGNILSVNTQMIRFRSDCIYKGIEENRNKVSKEMVLALDYLVKIIKNAS
                     DIQEFSAQDDGLIIIDNVNGLHAGTDYTDKNRHYIRARITV"
     gene            complement(4692..4799)
                     /locus_tag="WGR74_001617"
                     /pseudo
     CDS             complement(4692..4799)
                     /locus_tag="WGR74_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4845..5573)
                     /gene="pdxJ"
                     /locus_tag="WGR74_001618"
     CDS             complement(4845..5573)
                     /gene="pdxJ"
                     /locus_tag="WGR74_001618"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ0905995.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(5597..6388)
                     /gene="recO"
                     /locus_tag="WGR74_001619"
     CDS             complement(5597..6388)
                     /gene="recO"
                     /locus_tag="WGR74_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ0905996.1"
                     /translation="MSEHRINHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWVGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMPELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(6422..7549)
                     /gene="pheA"
                     /locus_tag="WGR74_001620"
     CDS             complement(6422..7549)
                     /gene="pheA"
                     /locus_tag="WGR74_001620"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ0905997.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACLTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASSSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(7588..8820)
                     /locus_tag="WGR74_001621"
     CDS             complement(7588..8820)
                     /locus_tag="WGR74_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ0905998.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLAVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRITAWRL
                     KTGAHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHNGSATVMAVTMTASTSCGIAL
                     LWLCSHRAWKENEQSEYL"
     gene            complement(9340..10194)
                     /locus_tag="WGR74_001622"
     CDS             complement(9340..10194)
                     /locus_tag="WGR74_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0905999.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT         2543 a         2870 c         2780 g         2481 t
ORIGIN      
        1 tcggccggat attaaaaaaa gccgtctgaa catttttcag acggcatttt tgtacacttt
       61 cctcaacggt tcgatccctg caccatatca atcaggaaca ggcggcaatc gaggttgccg
      121 ttataaagcg gaattttgcg ttttggcgac aggcgcatga atttgggcat ttccctgtcg
      181 ccggtaaaca tgccgacccg ccagccggca tagtgttttt tcaaccacgt acccaactgc
      241 ggataaagcg cctgcaaagc gtggacttcc tcaaggcgca cgccgtaggg cggattggac
      301 accataatgc cgttttcgcc gttcggtcgg acggactgcg cgtcggcaac gccgaaggaa
      361 acgatgtcgt ccacttcggc actgcgtgcg ttgtccaatg ccgtctgaac gatgcggcgg
      421 tcgctgtcgc tgcctgcaat cggggcgcgg acggggcggg tttgcgcttc ggcgcggcgg
      481 cgcaaatccg cccacagcgt tttatcgaaa ttttgcagtt tttcaaaacc gaagcggcgc
      541 atcatgcccg gtgcgcggcc tgtggcaatc caagcggctt cgatagcaat cgtgccgctg
      601 ccgcaaaacg ggtcttgaaa cggctgcgtg ccgtcgtagc ctgccgagag cagcagtccg
      661 gcggcaaggt tttcgcgcag cggggcttcg ccggtatcca ggcggtagcc gcgtttgaac
      721 agggcttcgc ccgaagtgtc gataaagatt tcgacattgc gttcgttcaa aaaggcgtgg
      781 atgcggacat cgggcgcggc tttgtccacg ctcggacgtg cgtcgtaaat gtcgcggaaa
      841 gcgtcgcaga cggcatcttt gacggtcagt ccgacaaatt ggaggctttt gacgttggca
      901 cgctttgcct cgattttgac tttgaacgtc tgctgcaagg taaaccagtt gaaccagttg
      961 atgtttttgg caagtttgta aatgtcgcgc tcggttcggt atgtgccttt ggtcaggcgt
     1021 agcaggatgc ggccggcggt acgcgagtgc aggttggcgg cgtaaacctg ttccaatccg
     1081 ccccggcagg aaacgccgcc gtcaaacgct tgaacatcgg tacagccgag gctttcgagt
     1141 tcttgcgata aaacggtttc caagccgcgc gggcaggtgg cgaaaagtgt atataaagta
     1201 ttcatagcgt gtcctttccg gacggatgtt ccggcaatgg cggattgaaa ggtttattcg
     1261 gcggtaaatg ccgtctgaaa tgtccggctg ccgattgtaa tgtccgccgt aaaatcgcgc
     1321 ctgccttcga cacagatggg caggcggatg cgtactgcct gccaagtccc cgacggttgc
     1381 cgctcgaaca tatagcggtt gaaacccata tccatatttt tcatactgaa gctgatgctg
     1441 acctgttccg tgccgacggg cgcgtgttcg atataaatat caaacggttt tttggttgaa
     1501 acggcggcgg cgcggacgcg gcttccgtcc ggcagcgtgc aaccctcggt caaatcgcat
     1561 tgcgccgcca cggcttgcgg ctgctgcgcc tgccaccatt gcaacaaaac gagcttgacg
     1621 gcggcaaagg caatcagcag cagtgcggca agcagtaatt tacggttttt attcatgatt
     1681 cttccctatt gtttcatccc ttaccaccac gtgcgccccc aatccgagcc atagttttcc
     1741 ataacgtgca accgtctgtc cgtttgctgc aagtacgagc ggcacggata cgccctgccg
     1801 cagggcttct gccgcctctt gtgcctgaac atcatcgcac acgcgccaaa cgaccgcatc
     1861 cgcgcccttt tcctgcgccc gccgccattg ttcgcgggtc tgcaccacca tccacacgct
     1921 gtcggcgtgt tcgggtctgt cctgccattg cgccgcctcc atcaaaacgt tcgcaccgct
     1981 tccctctgcc gaacccaaag gcaggacgcg gtacgcgccc atactgtttt ctccgtgcaa
     2041 acccgttttc aggctgccgt acaaacggcg cgggacggac agcgaacgca aaagcgcgcc
     2101 gttggcgggc agcatggggg caacggtaaa atcacccgcc ttctgccaag accattcctg
     2161 cccttcgcgg gattgcggtt tgcccgtcca ttggtcgggg ttgacccata ggaatttcag
     2221 gcagacgcgg gcgtgttcgt aggaatggat tttggtcagc caaggcgtgg cggcgaggat
     2281 gcggatgccg agttcttctt caaactcgcg ttgcaaggct tggaagtcgg tttcgcccgc
     2341 ttcgaccttg ccgccggcaa attcccaata tccggcatag ggtttgcctt cggggcgcga
     2401 gctgagcagg tagttgccgt ctgaatcgag caggatgccg gcaacgacgc ggataagggg
     2461 tcgggtgtct tgaatcatgg cgggtacgcg gttggtcggt cggagtgcaa atgagggacg
     2521 ggcaatgccg tcaacgggtt tgccgcattt cagacggcat tatttttcgg caacgacaaa
     2581 cgccaatacg gtgtcttctt cgtcgctcat gctgaggctg acgcggctga tgccttgttc
     2641 ctccagccat ttggacaggg cggggccgta gaaaaattcg ggcttgccca atgcgtcatg
     2701 cccgatgccg atgttgcgga aggaaaccgc gccgcgtatg cccgtgccga cggctttggc
     2761 aaaggcttct ttggcggcaa agcgtttggc gaggtagttg acgggtttgc ccgcttgcgg
     2821 aaattcaagc agctcttccg gagtgaggat gcgcccggca aacgcctgtc cgaatttttt
     2881 gtttaagcgg atgatgcgct tgagggaaac aatgtctgtg ccgatgccgt aaatcatatt
     2941 tgcgctcctt cgcccttggt tgcgggtagt gatgaatgtg atgatgaatg ccgtcaattt
     3001 cttggggcgt taaaatggcg tgaatatcca cttttggggt tttgtcggta acaattttca
     3061 ctgtaacctt ttgcatttga aattctaaaa catcgtgcaa attatttaat aaggagttaa
     3121 ggcatttaat aatgaattaa agtttgatta aacccgaatg cccgattttc agagccaata
     3181 ccaataaaac aataaaaatt aacattttaa aaaaatttgg gctaatctta gtccttaacc
     3241 gaattccaac atacaatcca ataacagaca atacggacag taaaaatatt aaaccgtatt
     3301 cactcttatt taataaccaa tactggtctc ttagcatata tatttgaaca attttcgcca
     3361 aaagatagca tagattgctt gattttacga tacgattttt attttctgtt tcgctaagca
     3421 aaaatattaa caatatggga gacatggcat tggttgaacc gccgatgatg cctgccaaaa
     3481 acccaaaaag aaccatattc ttattattgg caactacttg aatatttttt gcttttgcac
     3541 atacatttaa aataccattg acagaataat acaatgtaat gattgccatc agtaaaagca
     3601 gccaagacac tggaagtatc aaaagcaact tcaccccaaa aatgctgcca acgacgctgc
     3661 cgatagcaag caatttatag gtttttaaat aataaacaat ctcttgccaa aaaccttttt
     3721 tgttattgct gcatagaacc aacaagctca ttaacaggct tggtaatgcc accaaggcaa
     3781 caaccttaga caatggcatg ttaaaagcca atgcggttgt accgagcatc ggaaatccca
     3841 tgcctgtaat tccgtgcagt attgcggcag caacaaaaac gatagattgc attatttctt
     3901 gcataaccgt cctttataca gtaattcttg ctctaatata atgcctgttt ttatccgtat
     3961 aatcagttcc ggcatgcaag ccattgacat tgtcaataat aatcaaacca tcatcttgtg
     4021 cagaaaattc ttgaatatca ctcgcatttt ttataatctt tacaagataa tcaagtgcaa
     4081 gtaccatttc ctttgatact ttatttctat tttcttcaat acctttatag atacaatcac
     4141 ttctaaatct aatcatttga gtattgaccg ataagatatt accccatctc acaccttgtt
     4201 ttttatcaaa tgatgttggt gttttaaatg gatataaatt gcccgttaat gtttttaagt
     4261 gttttttacc agtttctgtt ttagataact gattgacaat atctgatgaa cttaaaaata
     4321 gggaattacc tccatcattg gctgatttta ctacatacat aaccaaataa ctttccgggt
     4381 tttcactaaa agatgaatca ctatgaagtg gacattctcc aacatcttcc gagaaagtta
     4441 tatcattatt tattacatag tcatcgccgc gatatttaat atcccaaaat atagatttaa
     4501 aaccatcgtc tttgtggttt gtcagcattc ctaattgaga tagaaagtca aataatttct
     4561 tgtttctccg gctgtcatca agatggttta gattaagacc gcgaacaata caatacccaa
     4621 tacgctgtac tgtattgtga atttcttcta tcaaactatt attgtttgca gataaaatat
     4681 acttttgatt catatagtga attaaattta aaccagtacg gcgttggctt gccttgccgt
     4741 actatttgta ctgtctgcgg cttcgccgcc ttgtcctgat ttaaatttaa tccactataa
     4801 catatccctg tcgtgaaatg ctgtctgaaa gggtttgcct gcccttacgg cagcagcctt
     4861 gccctgaaca tcgcctcctt catctggcgc acggcttcgg gcagtccgag gaagagggct
     4921 tgggcaatca gcgaatgccc gatgttcagt tcgcggatgg cgaggatttg ggcgatgggg
     4981 gtaacgttgt gtatggtcag tccgtgtccg gcgttgacga ccaagcccaa atcgccggca
     5041 aaatgcgcgc cgttttgcag acgctcgaat tgcttgagtt gttcggcgtg gctgcgcgcg
     5101 tcggcatacg cgcctgtgtg cagctcgaca acgggcgcgc cgacatcata ggcggcttgg
     5161 atttgcctgt cgtcggcatc gataaagaga gagactcgga tgcctgcgtc ggtcaggatt
     5221 ttggtgaacc cggcgatttt ttcctgttgc gccaatacgt ccaagccgcc ttcggtcgtg
     5281 atttcctgac gtttttcagg cacgatgcac acgtcttccg gcatcacttt aagcgcgttt
     5341 tcgagcattt cttccgtcaa cgccatttca aggttcaggc gcgtgcggat ggcgtttttg
     5401 acggcaaaca catccgcgtc tttgatgtgg cggcggtctt cgcgcaggtg catggtaatc
     5461 aaatccgcac cgtgcgtttc ggcaaccagt gccgcctcca cggggctggg ataagtcgtg
     5521 ccgcgcgcat tgcggacggt agcgatgtgg tcgatgttga cacctaaaag cataatcttt
     5581 ccttttattt ctgccttcag acggcatttg aagccgtgcc gtccgaagtc gggacggttt
     5641 cccgggcggt ttctttgcgg tcaaactgcc gtatctgttc caacacctgc cgcgatttca
     5701 gcccctcggg caacaggtgg cggataaaaa gccgtgtgat tttcaatgcc tgttgcaggc
     5761 tttcggcagt gcggaaactg ccttcgcgca aatcgatcag gctctgccct tcgacaattg
     5821 cgccggcttc gtgcgaaagc gcgtctgcgt cctcgcatac gggcatgacc gcctcttcgg
     5881 gcatcagccg gtaggttttg tccgccaaaa tatcgccgcc cgtgccgtcg gcgtgcaaat
     5941 cgggggcaac gcccaaggca ttgagcagct tccactcgaa gcggcgcaaa tcggcaatat
     6001 ggttggcttc acggcacacc gcctccatca cttttgccaa cgcgtcgtac agttcgggca
     6061 tcggatcttc gcgcgcggtc agtttcaaca ccagttcgtt cacatacaga ccgctgaaca
     6121 acgccctgcc ctgcggctgc cgccatccgc cgacccattc ggcacggtgc agggttttga
     6181 gttcctgact gccgtaccac gacgcactga cagggacgaa cggaaccaat acgccgcgca
     6241 gctcgctctg ccgtttgcgc gcgcttcttg ccaaaagtgc cacacgcccg taacggcggc
     6301 tgaaggcttc gacccgcagg ctgctttcgc gccagggcga tgatgccaac aggaagacgg
     6361 gttcgtggtt gatgcggtgt tcggacatca tgcctgaaat gccgtctgaa cgctgccgcc
     6421 gctacaaaac ggcggtcggg tacgaaccga tgactttgac gaacgaagcg cgttcgccca
     6481 agcgttccaa tgccgtctga atctgcgcgt cccggcggtg tccttcgatg tcgatgaaga
     6541 acaggtattc ccacaaaacg gatttgctcg gacggctctc aaacttggtc atggaaatac
     6601 ccgactcggt cagcggttgc agcagcgagg caaccgcacc ggcgcggttg ggcgcggaaa
     6661 cggccagcga agtcttgtcg ctgctgcttg caccggtttc gtgatgtccc atcaccaaga
     6721 agcgcgtggt gttgttcggt tcgtcttcga tgcactcggc aaccatatcg agtccgtaga
     6781 tttccgccgc cgtgcgtccg gcgatggcgg caaccgtacc gtcgtccgat tcggcaacca
     6841 gccttgcggc ttcggcattg ctggacacgg caatccgttc ggcgttgggc aggtgtctgc
     6901 ccaaccagtc gttgcactgc gccaacgcct gcgcgtggga aaagactttg gcaatgcctt
     6961 cggtgctgcc gttgttttta cgcaaaaggt tgtggtggat gcgcaaaacg acttcgccgc
     7021 acgcctgcaa cgcggtaacg gcaagcaaat ccaacgtgcg cccgaccgag ccttcggtcg
     7081 agttttccac gggggcgacc agatagtctg cctgacgggt ttcaacctgc ttgaagcagt
     7141 tgtctatggt cagacacgcc atggtgtgcg cggcgtgtcc gaaatgctta atcgccgcct
     7201 gctgggtaaa cgtgccctgc ggccccagat aggcgatggt cagcgggcgt tcgacggcga
     7261 ggcactcgct catcacttcc cgaaacaggc gtgctaccga ttcgtcgggc agcgggcctt
     7321 tgttcaaatc ctgaatgcgg cgcaacacgg cgacctcgcg ctccggacgg tatactgcgc
     7381 ccgtgccttt cagctcgccg atggcgtggg cgtgttgcgc gcgttcgttg agcaggcgca
     7441 ggatttcggc atcgatggtg tcgatggcgt tgcggtgggg aaggaggagt tcgtcgatag
     7501 tttgggacat aatttcgcct gaacagtaat ttatcgtgca ttctaacatc aaatgccatc
     7561 tgaaacggtt tcagacggca tttgatgtta aaggtattcg ctctgttcgt tttctttcca
     7621 cgcacgatgt gagcagagcc acagaagcgc aatgccgcaa gaggtggacg cggtcatggt
     7681 taccgccatc acggttgccg aaccgttgtg caagaaggtc gccgccatac ccacgcccgc
     7741 gccgattaag gactggaaca cgcccaacac ggcgttcgcg ctgccgccct cttctttgaa
     7801 ataagacata aagcatgcct gcgtgttcgc accgaccaag ccctgcgtgc cgacggaaaa
     7861 catcacgcac gcaaccagca gccaaaacgg gggcaacccg aaaaacagca cggcggcgag
     7921 ttgggacagg ttggcggcaa attggacgac aatcccccat agcaggatgc tttgcggatg
     7981 cgcgccggtt ttgagccgcc acgcggtaat gcggttgaaa aacatcatcg tgatgatgtt
     8041 gagtgcaaac gcccaagcgt attgatgagg cgtaacacgg taaagctgct ggtacacgaa
     8101 ggaagattcg gtcagaaagg cgaacatcga accgaagctg aatgcctgaa aaaacagata
     8161 acccatcgcg gcacgggttt tcaatacgcg tttgaaccgc ccagccacca gcccgaacac
     8221 atcccgtccg attttgcctc cgacggcagg cttgggcagg aaatactgca ccaaaccgag
     8281 cagcaccagc gaatacgccg ccagaaaaac aaaaatcgcc tgccagccac ccaagccctg
     8341 caacagtgcg ccgaccatgg gtgcgaccag cggcacaacc atcaaaatga tgccgataag
     8401 ggcaaacatc tgggcggctt tgcgtccgga ataataatcg cgcaccattg cgccgacgat
     8461 gaccacagtc atgcccgcac cgaatgcctg cacgacgcgc aggttgagga gctgttcggc
     8521 actcgaagca aatacgatgg cggcaacggc aaggcagtag acggccagtc cggtcagcgc
     8581 gacgggtttg cgccctttga tgtcggacac cgaaccgccg accacctgtc cgaacgccgt
     8641 gccgaacata aacaaactca ggctctgttc gatgcggtgg acatccgcgt tcagcgactg
     8701 cgccatttcg ggaatcgcgg gcaggtaggc atcgatggaa aacggcatca gcgtaaccag
     8761 catcgccatc aaaaccgcca tcagtttttc gctcatttca ggataatggg cagaaggcat
     8821 aaattctcgt ccgattcgca aacaaaccgc ccggcggcgg aaaacacaat acgcggaaac
     8881 ggaaaatttt aacacaccgg cacaaacagg cggcaacaaa agccccggca aaaccggacg
     8941 aaccggaacg atatagtgga ttaacaaaaa ccagtacagc gttgccttgc cttagctcaa
     9001 agagaacgat tctctaaggt gcttaagcac ccagtgaatc ggttccgtac tatctgtact
     9061 gtctgcggct tcgtcgtctt gtcctgattt ttgttaatcc gctataaaat cccgtcattc
     9121 ctgcgcaggc gggaatccgg acatccaacg ctgcagcaat ttgtcggaaa taaccgaaac
     9181 tcaaaaaacc ggatgcccgc ctgcgcggga atgacgatgc aaagatggtc gttatttcgg
     9241 ataaatatcc gtcttttcgt tccggatacc cgctttcgca ggaatggaga tggaaagatt
     9301 tcagcctatt cggcaatcat caaataccgc ttcccaactt tatttttgat gcgacagccg
     9361 gccggcaacc aaggtggcgg cgatgacgac aaacacgccc aaggcggaca cgggcgacag
     9421 gtgttcgccc aaaatcaaaa ccgccagcag cacgccgacg acgggttcga gcgaaatcaa
     9481 cagtcccgaa acgttggcag gaacacggct catccccttg ttccacagcc aataggcgta
     9541 ccagccgcac cccaaaccca aatacagcag cgacaatacc atcccgacgc tccagtccac
     9601 ggtataactt tgcgccaaag caagcgaaaa cggcaggcac atcaacgatg cggcggcaat
     9661 ggaaacagat gtgaatgccg gtgcgccgat gcgtgcaatc agcctttgcg tcggacgcat
     9721 agcggcacaa aagcccgcgc ccgccaacaa caccagcagg cagccgaacc agccgacttc
     9781 gccgccctct tccgcaccgc ccgccatcag cagcgcgaca ccggcaaatg ccgccgcgcc
     9841 gcatatccag tggtaggcac gcgctttgtc gttgaagaaa aagtgtccga caaacaccat
     9901 cagcagtggc tcgagtccga caatgaccga tgcgctggcg gcggaagtgt atttcaaccc
     9961 gacaaactga agcagcaggg tcagcacata gttgacgaac gacacaatca gcaacggctt
    10021 ccattcctca cgcggaatct tgccgacatg acggcggcag gcgggcagtg caggcagcgc
    10081 ggcaattagc aggcgcacgc cgaccatcaa tgcgggatcg atgccgccat agacatattt
    10141 ggcggcaata aacgagctgc tccagataat cagggcaagg atttggtaaa acatagtgag
    10201 tgataaagtc agatggtcat acaaaagtat agcggatgaa caaaaatcag gacaaggcga
    10261 cgaagccgca gacagtacag atagtacgaa accgattcac ttggtgcttc agcaccttag
    10321 agaatcgttc tctttgagct aaggcgaggt aacgccgtac tggtttttgt tcatccacta
    10381 taacagcaac cctgtcgccg tcattcccgc aaaagcggga atccagtccg ttcagtttcg
    10441 gtcatttccg ataaatgcct gctgcttttc atttctagat tcccactttc gtgggaatga
    10501 cggcggaagg gttttggttt tttccgataa attcttgagg cattgaaatt ccaaattccc
    10561 gcctgcgcgg gaatgacgat tcataagttt cccgaaattc caacataacc gaaacctgac
    10621 aataaccgta gcaactgaac cgtcattccc gcaaaagcgg gaatctagaa tctc
//
LOCUS       DAWFLT010000065        10425 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWFLT010000065 DAWFLT010000000
VERSION     DAWFLT010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10425
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.065"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..297)
                     /locus_tag="WGR74_001623"
     CDS             complement(<1..297)
                     /locus_tag="WGR74_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0906000.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRH"
     gene            536..1435
                     /gene="crgA"
                     /locus_tag="WGR74_001624"
     CDS             536..1435
                     /gene="crgA"
                     /locus_tag="WGR74_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ0906001.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1660..4875)
                     /gene="carB"
                     /locus_tag="WGR74_001625"
     CDS             complement(1660..4875)
                     /gene="carB"
                     /locus_tag="WGR74_001625"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ0906002.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VNTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4882..5430)
                     /locus_tag="WGR74_001626"
     CDS             complement(4882..5430)
                     /locus_tag="WGR74_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906003.1"
                     /translation="MRTIFFSGSRSISRLNPQIRERINNILSNNFDIVIGDANGADKA
                     IQKFLQEQDYANVHIYFSGKIYRNNVGNWQFVQVDSKGTGRVFYTAKDKKMAEIADYG
                     FILWDGKSIGSLNNIAELLQLNKPSLVYHSQTKEFFKIKSSADLENILSNIEDDVLAS
                     ILEKGNTFLKSYVTKQPSLIQE"
     gene            complement(5433..5996)
                     /locus_tag="WGR74_001627"
     CDS             complement(5433..5996)
                     /locus_tag="WGR74_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ0906004.1"
                     /translation="MAIELNGNWTKGFALDHHMQKSILLGYDELGHPHFDSKRSVIGE
                     WVYQLKYRNQTQNVSLLVDYILQKFSGLESINLIVPAPFTTERINQPVQLIAKELSDR
                     LNIPYSPILRKSSSHTPLKNIEEKSEKLEILNNSITIDNIDLSNKNILVIDDLFDSGA
                     TLEISTEKLFSKNARSVIVLAMTKTRG"
     gene            complement(6848..7237)
                     /locus_tag="WGR74_001628"
     CDS             complement(6848..7237)
                     /locus_tag="WGR74_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ0906005.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSGAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(7810..7935)
                     /locus_tag="WGR74_001629"
                     /pseudo
     CDS             complement(7810..7935)
                     /locus_tag="WGR74_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7981..9114)
                     /gene="carA"
                     /locus_tag="WGR74_001630"
     CDS             complement(7981..9114)
                     /gene="carA"
                     /locus_tag="WGR74_001630"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ0906006.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYASGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         2384 a         2677 c         2653 g         2711 t
ORIGIN      
        1 gtgtctgccg tcgctttggt agagtttgcc gtgtccagcg gttaatactt cgtctatgta
       61 ttttttcact gtccaaggct cgtgcatcca ccagcccggc atctgccaaa tcacggcatc
      121 catccaaacg aatttttcga tttctgcctc aacatcatag ccggcatcaa tcacggtttc
      181 ttgaacattg tgtccgagcg cggtcaaaac ttctttcgct tttttgtgaa gcgtgcggtt
      241 taacccgccg tgagaatgtc cgaacgcctt gccgccgtct aataataaaa tattcatttg
      301 ttacctcgtt tgtgaattga tgaatgtatt gtgccgtttt actttataat ttaaaagtgc
      361 aaaaataaga aaacactttt gcgtcaaatg aaataatcag atgtttcaac acacgggatg
      421 gcacataaag caccgccctg tgtgccgtcc tgattcggaa ggggttacac ccctcccaaa
      481 taaagtctga tcctgccgcc ctaaagggcg gggttccaac cgaaaaggaa acacgatgaa
      541 aaccaattca gaagaactga ccgtatttgt tcaagtggtg gaaagcggca gcttcagccg
      601 tgcggcggag cagttggcga tggcaaattc tgccgtaagc cgcatcgtca aacggctgga
      661 ggaaaagttg ggtgtgaacc tgctcaaccg caccacgcgg caactcagtc tgacggaaga
      721 aggcgcgcaa tatttccgcc gcgcgcagag aatcctgcaa gaaatggcag cggcggaaac
      781 cgaaatgctg gcagtgcacg aaataccgca aggcgtgttg cgcgtggatt ccgcgatgcc
      841 gatggtgctg catctgctgg cgccgctggc aacaaaattc aacgaacgct atccgcatat
      901 ccgactttcg ctcgtttctt ccgaaggcta tatcaatctg attgaacgca aagtcgatat
      961 tgccttacgg gccggagaat tggacgattc cgggctgcgt gcacgccatc tgtttgacag
     1021 ccgcttccgc gtaatcgcca gtcctgaata cctggcaaaa cacggcacgc cgcaatctac
     1081 agaagagctt gccggtcacc aatgtttagg cttcaccgaa cccggttctc taaatacatg
     1141 ggcggtttta gatgcgcagg gaaatcccta taagatttca ccgcacttta ccgccagcag
     1201 cggtgaaatc ttacgctcgt tgtgcctttc aggttgcggt attgcttgct tatcagattt
     1261 tttggttgac aacgacatcg ctgaaggaaa gttaattccc ctgctcgccg aacaaacctc
     1321 cgataaaaca caccccttta atgctgttta ttacagcgat aaagccgtca atctccgctt
     1381 acgcgtattt ttggattttt tagtggagga actgggaaac aatctctgtg gataattcta
     1441 taaaaacctg tcataaaact gtttttactc acttaataac tttgagaaaa aataaataag
     1501 atgatgtata gccggcggca atggcagtct atatggggag cgtgataggg ctgttcctta
     1561 tgtacggctt gtgtgtggca acatcggctt tccaaatgac aaaatgccgt ctgaaaccgc
     1621 aaaacggttt cagacggcat tttcaacctg attcaggcat cagttgcggt ttttcaaacg
     1681 cccgtgcagc tcttgaacgc tgtacacgcc cagatggtct cggcttttcg cgccttcgct
     1741 catcgcttcg ccgccggcag tagtggtgta ttgcggcaca cgttgctgca atgcgctttg
     1801 gcggatgatg tggctgtcgg acacggattg cggatcgctg gaaacggtat tcacgaccag
     1861 tgcgatttcg ccgtttttca gcgcgtcgcc gatgtgcggg cggccttcgg ggactttgtt
     1921 gatggtctgc acaattagcc cgtgttcggt caggtattgc gccgtgccgc gcgtggcgca
     1981 gatgccgtag cctaaggctt ggaagttttt ggcggttttg atgacgcgtt ctttgtcttc
     2041 ttcgcgcacg gagaggaaga ttttgccggt cgggttgagg cgttcgcctg cgccgagttg
     2101 ggctttgtag taggcttcgc cgaagcttgc gcccacgccc atgacttcgc cggtggagcg
     2161 catttccggt cccaaaatcg tgtccacgcc cgggaatttg atgaatggga acacggcttc
     2221 tttaacggca tagaaatcgg ggacaacttc tttttccacg ccttgttctt tcagggaaat
     2281 acctgccatg cagcgtgcgc cgactttggc gagcggcacg ccggtggctt tggagacgaa
     2341 gggcacggta cggctggcgc gcgggttcac ttccaacacg aacaccacgc cgtcctgcac
     2401 ggcaaactgc acgttcatca gaccgaccac gcccagcgcg tacgccatcg ctttggtttg
     2461 gcggcggatt tcgtcttgga tttcttcgct taaggagtag ggcggcagcg agcagccaga
     2521 gtcgccggag tgaatgcctg cctgttcgac gtgctgcata atgccgccga taaccacgtc
     2581 tttgccgtct gaaacgcagt ccacatccac ttcaatcgcg ttgttgagga agaaatcgag
     2641 caacacgggg ctgtcttcgg aaacctgcac ggcttcgcgc atgtattttt gcaaggcttc
     2701 ggcagagtgg acaatctgca tcgcgcgtcc gcccaaaaca taagacgggc gcacgaccag
     2761 cggatagccg atttcttcgg ctttgacgag tgcttcttct tcgttgtggg cgatgcggtt
     2821 gggcggttgg cgcagaccta agtcgttcaa cactttttgg aagcgttcgc ggtcttcggc
     2881 ggcgtcgatg ctgtcggcgg acgtgccgat gatgtttacg ccgttttcaa ccaacgcgtt
     2941 ggcgagtttc agcggggttt gaccgccgta atgcacaatc acgccccacg ggttttcggt
     3001 gcggacgatt tccaacacgt cttccaatgt cagcggctcg aaatagaggc ggtcgctggt
     3061 gtcgaagtcg gtggacacgg tttctgggtt gcagttgacc atgatggttt caaagcccga
     3121 ttcgcgcagg gcgagtgcgg cgtgaacgca gcagtagtca aactcgatgc cctgaccgat
     3181 gcggttcggg ccgccgccga gaatcatcac ttttttacgg tcggaaggac gggattcgca
     3241 ttcctcttcg taagtggagt agaggtaggc ggtttcggtg gcaaactcgg cggcacaggt
     3301 gtcgacgcgt ttgtaaaccg gatgcagctt cagcgcgtag cggtgttcgc gaacttcttt
     3361 ttcgcttacg ttcaacaatt gtgccaaacg tttgtcggag aagcctttgc gtttcagacg
     3421 gcgtagggcg gcgaaatcca aatcactcaa aatgccgtct gaaaccgctt tttcttcctt
     3481 catcaagtct tcgatttgcg ccaggaacca agggtcgatg gcgcagattt cgtggatttc
     3541 ttccagcgtg aagcccgcgc ggaacgcgtc tgccacaaac agcatacgtt cggggccggg
     3601 gtttgccagt tcgcggcgga tttccgcttt gtcggagctt ctcggattga agccgcacaa
     3661 gcctgtttcc aagccgcgca gggctttttg gaagctttcc tgaatcgtgc ggcccatcgc
     3721 catcacttcg cccacggatt tcatctgcgt ggtcaggcgg tcgtctgcgg cagggaattt
     3781 ttcaaatgcg aaacgcggga ttttggttac cacatagtcg atggaaggct cgaacgacgc
     3841 gggggttttg ccgccggtga tgtcgttgcg caactcgtcc agcgtaaagc cgaccgccag
     3901 ttttgccgcc accttcgcaa tcgggaagcc cgtcgctttg gaagccagcg cggacgaacg
     3961 gctcacgcgc gggttcatct caatcacaat catctcgccg ttttcagggt tcaccgcaaa
     4021 ctgcacgttc gagccgcccg tgttcacgcc gatttcgcgc aataccgcca acgaagcgtt
     4081 gcgcatgatt tggtattctt tgtccgtcaa cgtttgcgcc ggcgcaaccg taatcgagtc
     4141 gcccgtatgc acgcccatcg ggtcgaagtt ttcaatcgag cagatgatga tgcagttgtc
     4201 gtttttatcg cgtacgactt ccatctcgta ctctttccag ccaaggacgg actgctcaat
     4261 cagcagctcg tgcgtgggcg aggcatcgaa accgcgttcg caaatcgcca aaaactcgtc
     4321 tttattgtag gcaatgccgc cgcccgaacc gcccatggtg aaagaaggac gaatcagggt
     4381 agggaagccg acctgttctt gcgccgccaa agcttcgttc atggtgtggc agacaaaaga
     4441 tttcgggcaa gataaaccga ttttttccat cgcctcctta aagcgaccgc ggtcttctgc
     4501 tttgtcgatg gcgtcttccg tcgcaccgat caactcgaca ttgtatttcg ccagcacgcc
     4561 gttgcgcgcc aaatccagcg cacagttcag cgcggtctga ccacccatcg tgggcagaat
     4621 cgcatcgggc cgctccttgg cgataatctt ctccaccgtc tgccacataa tcggctcgat
     4681 gtaggtaaca tccgccattt cagggtcggt catgatggtg gcggggttag aattcaccag
     4741 aatgacttta tagccttctt cacgcaaagc cttgcaggcc tgtgcgcccg aatagtcaaa
     4801 ttcgcaggcc tgaccgataa cgatagggcc ggcgccgatg ataaggatgg attttaggtc
     4861 ggtacgtttg ggcatgggtg gttactcttg aattaaagaa ggttgttttg ttacatagct
     4921 tttaagaaaa gtatttccct tctctaaaat tgaagctaaa acatcatctt ctatgttaga
     4981 taaaatattc tctaaatctg cgcttgattt aattttaaag aattcttttg tttgagaatg
     5041 gtatacaaga gaaggtttat ttagctgtaa aagttctgct atattattta atgaaccaat
     5101 actcttacca tcccataaaa taaagccata atcagcaatt tctgccattt tcttatcttt
     5161 tgctgtataa aatacccttc cagttccttt gctgtctact tggacaaact gccaattccc
     5221 cacattattt cggtaaattt tgccagaaaa gtaaatatgc acattagcat aatcttgttc
     5281 ttgtagaaat ttttggatag ctttatcagc cccattagca tctccaataa caatatcaaa
     5341 attattgcta agaatattgt taattctttc tcgtatttgt ggatttagtc gtgaaatgct
     5401 gcgtgaacca gaaaagaaaa tagtgcgcat ttttatcctc tagtctttgt cattgctaaa
     5461 acaattacag atctagcatt ttttgaaaac aatttttcag ttgatatttc caatgttgct
     5521 ccgctatcaa ataaatcatc aattactaaa atattcttat tggatagatc tatattgtca
     5581 attgtgatgg aattattcag tatttctagt ttttctgatt tttcttcaat atttttgagt
     5641 ggtgtgtgtg agctagattt tcttaaaata ggagaatatg gaatattcaa gcgatcactt
     5701 agttctttgg caattaattg aactggttga ttaatacgct ctgttgtaaa aggggcggga
     5761 acaattaaat ttatagattc taaaccacta aatttttgta aaatatagtc taccagcaaa
     5821 gaaacatttt gggtttgatt acgatatttt aattgataaa cccattctcc aatcacactt
     5881 ctcttgctat caaagtgagg atgccctaat tcatcataac ctaacaaaat acttttttgc
     5941 atatgatggt ctaaagcaaa tccttttgtc cagttaccat ttagttctat cgccatttca
     6001 ttgactcctt gtcaaaatta agtataacga gcaatcctag caagccgtag cccgcatgta
     6061 gggtatgtgc ggtacgcacg cacgcgttct tcattttccc tgtaacccca acccaccgca
     6121 tgcgcgcctt gcggcacaca ctctgccgac ggattcaaag gtcgtctgaa aaaacgctcg
     6181 cggcgtttgt tacatctgcc ttgcgataaa ggctgcctga aaaattccca ctgttgatat
     6241 ttcgggcatt ctttctcaaa ccgcctgcaa ccgcaaaatc cgctgcaacc tgccccctcc
     6301 cccgtggggg agggctgggg agagggcatt ccccgaattt cggcaacctt tcccaacaac
     6361 tcaaccaccg aaatacaagc cttgtggctt gttgccctct ctctagctct ctcccacggg
     6421 agagaggacg gagtggctgt agggctaagg gttctgcaag gaaacaggtt gtgcaggttg
     6481 ctttttgctt ttcagacgac ctgaaatcaa cggtaaatcc aagtcgcttt tcaaaccgcc
     6541 tgcaaccgca aaatctgctg aaacctactc cctcccccgt gggggagggt tggggagagg
     6601 gcattccccg aatttcggca acctttccca acaactcaac caccgaaata caagccttgt
     6661 ggcttgttgc cctctctcta actctctccc acgggagaga ggactgggcg gttgttgggg
     6721 ctaaggtttc tgtaaactaa tcaggttatg caggctgctt tttgtttttc agacggcctt
     6781 tataaatgct caattcagtc ggctttatcc tattattttc aataatccaa tcaaaatcag
     6841 ccatctgcta tcgtgcagct tgcttttcca attcttgcaa cacgagcagg atttctgtca
     6901 gcacatcgtt tgtctgctgt aaaatctcgt gattccaaaa ccgcagtacg gtaaagccca
     6961 agctgttgag atatgccgtc cgcgcgtggt cgtatgcggc ttgttctgtg tgctgccctc
     7021 cgtctgcttc gacaatcaac ttgggcgtta cgcacataaa atcaacaata tagtttccca
     7081 tcggctgctg gcggcggaat ttatagccgt tcagacgacc tgcccgcagg tgctgccaca
     7141 attttgcttc cgccccgctc atttcttggc gcatggcttt ggcgcgttgg cgcaagatgg
     7201 ggttgtcggt ggtcaagagt ttctcgtgcg ggttcatttt gttgtcgtgc tgtttgctgc
     7261 cctctctcta actctctccc acggggagag aggacggggc ggttgttggg gttaaagggg
     7321 ctgtaaggaa aacaagtcgt tcaggccact ttttactttt cagacgacct gcaatcaacg
     7381 gttgtaaatc taagttgcct ttcaaatcgc ctgcaaccgc aaaatctgct gcaaccttct
     7441 ccctcccccg tgggggaggg ctggggagag ggcattcttc gaatttcggc aaccttcccc
     7501 aacaccctaa ccgcccaaac acaagccttg ctgtttgttg ccctctctct aacgctttcc
     7561 cacagggaga caggacgggg cggctgtagt gattaagggt tttgcaagga aaacaaggaa
     7621 aacaagttgt acaggctgtt ttttattttt caagcgacct gaaatcaacg cttgtaaatc
     7681 caagtcgctt ttcaaactgc ctgcaaccgc aaaatctgct gcaacctacc ccctcccccg
     7741 tgggggaggg ctggggagag ggcattttcc aagttgcggc aatctttccc aaatccctta
     7801 gtcttctaaa atacaagcct tacggttgtt gccaaatgaa acttcttcca tacccaaaac
     7861 ttgcgtgtaa aacgcaatgg ttcggtcaat gtcggcaacg gtcagtacca gatggtcgag
     7921 tgcgctaatc ttcatttccc gctttctttt tcaggcggcg gcagtgccgt ctgaaagcca
     7981 ttatgcccgt tttgccgctt tcatattgcc aatgaatttg tcaaacaaat agccgacatc
     8041 ttgcggaccg gggctggctt cggggtggcc ttggaagcag aacacaggtt tgtcggtcag
     8101 ctcgatgcct tgcaaagtgt tgtcaaacaa ggatttgtgg gtaatgcgtg cgttagcggg
     8161 cagggtgtcg gcatcaacgg caaaaccgtg gttttggctg gtgatgacga ctttgccgct
     8221 gtccaaatct tgcacagggt ggttcgcacc gtggtggctg aagcgcattt tcagggtttt
     8281 cgcgccgata gcgaggctga tgagctggtg tcccaagcag atgccgaaaa tcggtttgcc
     8341 gctttccatc agtttttgca cggcttcgat ggcgtaggtg caaggctcgg ggtcgccggg
     8401 gccgttggac aggaacacgc cgtcgggatt gagtgccaac acgtcttgtg cgctcgtttg
     8461 ggcggggacg acggtcaggc ggcagccgcg cgaggcgagc atacgcagga tgttggtttt
     8521 cacgccgaaa tcgtaggcga cgacgtggta aggctgttcg tcaggggtaa cgaaaccctt
     8581 gcccaattcc cattcgcctt ccgtccattc gtaagtttcc gtgcaggaaa cttcttttgc
     8641 caagtctttg ccgaccatac tgccgaacgc ggcgatgagt tcttgcgctt tttcgattgt
     8701 ggcatccgca ccggtcagaa tcgcgccgcc ttgcgcgcct ttttcgcgca acagcgtggt
     8761 caggcggcgg gtatcgatgt cggcgatggc gacggttttg ttgcgtacca gatagtcgtg
     8821 caggctttcg gaggcgcgga agttgctgtg caagagcggc aggtcgcgga taatcaggcc
     8881 ggaggcataa acgctgcggc tttcttcatc ttcggcgttg gtgccggtgt tgccgatgtg
     8941 tgggtaggtg agggtaacga tttgtttgca gtaggacggg tcggtcagga tttcctgata
     9001 gccagtcatc gaagtattga acacgacttc gccggaagtc gaaccttcgt aaccgattga
     9061 tgtgccgtgg aatacgctgc cgtcagcgag gacgaggagg gcgggggtgc tcatgatggg
     9121 aatcctgttt ttaataaaat tcttgacaat gccgtctgaa gggacttcgg cagggggcat
     9181 ccggtcggaa aaatccggtc aaaaaaaaac acgccgcgca agatagacgc gtaacgtgct
     9241 tttcgggcaa gtgcctatac cctgaaagca gcatatttta agacgaaaca tacccgattt
     9301 caagcgcaaa cgcggcaggc gggctgatat gttcgcgcga aaacggaaat ccgtggcgta
     9361 aaaccgcccg ctccgcgccc actatgcccg ttacgcattt ccccacgtcc gtccggcgaa
     9421 actatgggaa tacccgaatc gtcaggaata cctgaatcgt caggaatacc cgaatcgtca
     9481 ggaatacctg aatcgtcagg aatacctgaa tcgtcaggaa tacccgaatc gtcaggaata
     9541 cccgaatcgt caggaatacc cgaaccatca ttcccgcgca ggcgggaatc cagacctgtc
     9601 ggcacagaaa cttatcgggt ataacggttt cttgagattc cgcattctag attcccgcct
     9661 gcgcgggaat gacggcatcg gtttgacggt atttaattga attgcggaaa ttgacggatt
     9721 cagtgagatt ggcgggatga agcctaccct atagcccgcc ttttatagcg gattaacaaa
     9781 aaccggtacg ggttgccccg ccccggctca aagggaacga ttccctaagg cgcccaagca
     9841 ccaggcgaac cggttccgta ccatttgtac tgcctgcggc ccgccgcctt gtcctgattt
     9901 ttgttaatcc gctatacgaa cccgccccct cccgaaaaac gcaaaaaatg ccgtccgaaa
     9961 acctttcgga cggcattttc gcgtgaaccg tcattcccac gaaaacagaa aaccaaaatc
    10021 agaaacctaa aattcgtcat tcccacgaaa gtgggaatcc aggactcagg acctgagaaa
    10081 cctttttacc cgataagttc ccgtaccgac cggtccggat tcccgcctgc gcgggaatga
    10141 cgcgtttcat ttgcccccat cccgaaaacg caaacaatac cgtctgaaag ctctgtaggg
    10201 agggcttcag cccaccgatt ccatcaatcc cacatttccg ccgttttcac cgaatccgcc
    10261 ccttttcaca atccagtcct acgcaactga accgtcattc ccgcgcaggc gggaatccgg
    10321 acttgtcggc acggaaactt atcgggtaca acggtttctt gagattttac gtcctagatt
    10381 cccgcctgcg cgggaatgac ggcggagcgg tttctgtttt ttccg
//
LOCUS       DAWFLT010000066        10256 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWFLT010000066 DAWFLT010000000
VERSION     DAWFLT010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10256
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.066"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(26..349)
                     /locus_tag="WGR74_001631"
     CDS             complement(26..349)
                     /locus_tag="WGR74_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ0906007.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(337..576)
                     /locus_tag="WGR74_001632"
     CDS             complement(337..576)
                     /locus_tag="WGR74_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ0906008.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(699..1496)
                     /gene="truA"
                     /locus_tag="WGR74_001633"
     CDS             complement(699..1496)
                     /gene="truA"
                     /locus_tag="WGR74_001633"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ0906009.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTTVAGRTDTGVHATAQVVHFDTTAARPQQAWIRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRQAAAYLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSAGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
     gene            complement(2302..2487)
                     /locus_tag="WGR74_001634"
     CDS             complement(2302..2487)
                     /locus_tag="WGR74_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906010.1"
                     /translation="MPEQARARPIQTAMTLCRRFGAGWRHTRRLPRFISHRSSETRNP
                     PGPTCGMKRLSWREVFY"
     gene            complement(2598..3290)
                     /locus_tag="WGR74_001635"
     CDS             complement(2598..3290)
                     /locus_tag="WGR74_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0906011.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPAPSSLTSAKTFWGV
                     CRKTTGIRFNWRLVGAPEYVADYVCIHELCHLAHADHSRAFWALTRRFAPYTPEAKQW
                     LKIHGRELFALG"
     gene            complement(3287..4030)
                     /locus_tag="WGR74_001636"
     CDS             complement(3287..4030)
                     /locus_tag="WGR74_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0906012.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     GKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            complement(4059..4622)
                     /gene="gmhB"
                     /locus_tag="WGR74_001637"
     CDS             complement(4059..4622)
                     /gene="gmhB"
                     /locus_tag="WGR74_001637"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ0906013.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            complement(4619..5191)
                     /locus_tag="WGR74_001638"
     CDS             complement(4619..5191)
                     /locus_tag="WGR74_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0906014.1"
                     /translation="MNYKIYDGAKIIKKENLSIGDYSQIDDFVFFNCGEKSILGSFVH
                     ISSFTSIIGGGEFYMDHFSGLSAGCRIITGSDDFMGGGGLTNPTVPSKYTNVKKSSVR
                     IGKHAILGTNVTVLPGVTIGDGVAVGAGAIVRKNLEPWTIYVGVDCKPLKRRSQDKIL
                     ELEKHLVAEFNLDLCPIIPSPTRPNPKETR"
     gene            5361..7013
                     /locus_tag="WGR74_001639"
     CDS             5361..7013
                     /locus_tag="WGR74_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ0906015.1"
                     /translation="MVAYVFLFLFVTAALVLIIRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIHYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADSLDVPWRWAGAVLFAAAFVSMRHFSKHPGAVKTWLDSL
                     PDDGRDVFLNLPMSCRAVFFQVPVFEGDGEAFARQMPSETRPCGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGFVRNLREHGYFCVALSPFTKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDNVFDLDAPDLNAKTVSALNDYIGRIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQNFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNWELVGNYRNYLYDVLKIAR"
     gene            complement(7083..8324)
                     /locus_tag="WGR74_001640"
     CDS             complement(7083..8324)
                     /locus_tag="WGR74_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ0906016.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRSWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSAAGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKLPVLFDTQAPTGTNYWIYVATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            8476..9204
                     /gene="ubiG"
                     /locus_tag="WGR74_001641"
     CDS             8476..9204
                     /gene="ubiG"
                     /locus_tag="WGR74_001641"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ0906017.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACANLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            9241..10158
                     /gene="thrB"
                     /locus_tag="WGR74_001642"
     CDS             9241..10158
                     /gene="thrB"
                     /locus_tag="WGR74_001642"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ0906018.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYNAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKVFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
BASE COUNT         2184 a         2417 c         3078 g         2577 t
ORIGIN      
        1 aacacaatcc attcagacta ttcaatcagg caaacatctc ctgcaatact gcaaacagtt
       61 tttcagccgt actgttgtct aaatttccaa gatgtttgac caatccggct ttatccacag
      121 ccctaatctg ttcgggcaaa agcaaaccgt ctttatcctg aaagcggaca ttgacgcgga
      181 acggggcagg acggcttccg ctcgtcatag gaacgatcag cacagtcttg agatagttgt
      241 gtatttcagg aggagatact acgacacaag gacgtgtctt tttgatttcg cttcctacag
      301 tcgggtctaa ggagaccaga tagattccgc cgcgtactac catatccatt ctttatccgc
      361 ttcgttttca atttcggaaa aaaaatgctc ctgctcggtt tcgacaagca ttgcggcagc
      421 ttctgcccat cctctgcgaa cggtaggaca gcttaaaata atattgccct tttcaactgt
      481 aacagccaag ctgtccactg cccctatttg acccaataat gatttgggca gaatcactcc
      541 ttgcgagttt cccattttgc gtatgttgag aatcatatgc gtaccatatt taccagttta
      601 tgtaataaca atgttattac cttgatgagg tagtgtcaat attgaaaaag attgcctgac
      661 agtttgtccg atttcaaaat ctccgcgaca agaatgtttt aaagccattc ggggatttgg
      721 gggcggatga tgccgtatgc ctcgggatag tcgacgccgg tcaggtaaag cccgtcgggc
      781 atgaaggtcg gtggggcttt gaggcggctg cgttcttgaa tgagggcggc gaagccttcg
      841 acgctgagcc tgccgctgcc gacataaacg agcgcgccca tgatgttgcg taccatatgg
      901 tgcaaaaagg cgttgccgtg caaatcgagg cggacgagtc ctgcgctttg ggtaaggtcg
      961 gcgcggtaaa tggttttgac gggggatttt gcttggcact cggcggcgcg gaagctggag
     1021 aagtcttgtt cgccaatcaa ataggcggca gcctgccgca tcggttcgat gtcgagtttg
     1081 aggtgtgtcc agcctgccct gtttttgagc aggggggaac ggacgggggc ggattcgagc
     1141 aggtagcggt agtgccgtcc gtatgcgtca aatcgtgcat gaaattcggg ggcgacctgt
     1201 cgggcgtgca aaacggcaat gccttcgggc aggtgggcat ttacgccgcg tatccatgcc
     1261 tgttggggac gggcggcagt tgtgtcgaag tggacgactt gggcggtggc atgcacgccg
     1321 gtgtcggtcc tgccggcaac ggtggtggaa accgcttccc ctgctatttg ggcgagcgcg
     1381 gtttccaatg ccgcctgaac ggtcggtacg ccgtcagcct gtttctgcca gccgtaaaag
     1441 cggctgccgt cataggatag ggttattgcc cagcgttgtt tttgtgcggt atccatcgga
     1501 tttgggattc ggataaatgt tcagactgca ttgtatcgca gattttgcag ggaaacggca
     1561 aacgcccagg gcgagcggcg ttgtttgggg agttgttggg ggggggggat gcagttgcta
     1621 cgaatcgcta tcctgtgaat tcaccctgtc aggagtgccc gaatcgtcat tcccgcgcag
     1681 gcgggaatct aggacgtaaa atctaaagaa accattttat agtggattaa caaaaaccag
     1741 tacagcgttg cctcgcctta gctcaaagag aacgattctc taaggtgctg aagcatcaag
     1801 tgaatcggtt ccgtactatc tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt
     1861 taatccacta tatccgataa gtttccgcac cgacaaaact agattcccac tttcgtggga
     1921 atgacgggat gcaggttcgt gggaatgacg cgaacagaaa cctcaaatcc cgtcattccc
     1981 gcgcaggcgg gaatctagac cttagaacaa cagcaatatt caaagattat ctgaaagtct
     2041 gggattctgg attcccactt tcgtgggaat gacgggatgt aggttcgtgg gaatgacgcg
     2101 gtgcaggttt tcttaacccc gcgttctaga ttcccacttt cgtaggaatg acggcggtag
     2161 atttggcaga tgcggcggat tcggcaggtc tcaacccatc ctacaatcca ccctgaccgc
     2221 cttttacgaa tccgccgcca tcctgggaaa cgcaaaaaaa tgccgtccga aaacctttca
     2281 gacggcattt tcgcgggcaa atcagtaaaa gacttcgcgc cagcttaagc gtttcatacc
     2341 gcacgtcgga ccgggcgggt ttcgggtttc ggaagagcgg tgtgaaatga aacgcggcag
     2401 acggcgcgtg tgccgccatc ctgccccgaa ccggcggcaa agcgtcatcg cggtttgaat
     2461 cgggcgggcg cgtgcctgtt ccggcacgga tgccgtctga aagcggcggc agcggtatgt
     2521 gtcgccggct gtgatgctgc tttccggatg tccgtttggg aggaggtctg aattggcgga
     2581 tgcctgcccg ggccgcgtca gcctaaggcg aaaagttccc tgccgtggat tttgagccac
     2641 tgtttcgctt cgggcgtgta gggtgcgaag cggcgggtca gtgcccagaa cgcgcggctg
     2701 tggtcggcgt gggcgaggtg gcagagttcg tgtatgcaga catagtcggc aacgtattcc
     2761 ggtgcgccga ccagccgcca gttgaagcgt atgcctgtgg ttttgcggca cacgccccag
     2821 aaggttttgg cagaggtcag cgaggagggg gcggggaaca gttgtgtggt gcgggcgtgg
     2881 cgttcgaggc ggggaatcag gtaactgtgc gcctgccgtt ccaaaaagtc ccgcagcagc
     2941 gcaagctgtt tttcgggtgc gccttcggga acacggattt cagacggcat cagcaggatt
     3001 tgcgtgtctt gatgggcggt gagggcaagc tgtctgccgt ggaagaggat ggattcgggc
     3061 agccggtttt cggcagtttg cggcggcggt gttttcgcca gtgtttgccg caggacggct
     3121 tcgttttcat acagccagcg gtttagagcg gagacggaga agcagggtgg gacgctgatg
     3181 cggacggtat gtgtgccggc ggggcggata atcaggtttt tcttggcacg gcgcttgatt
     3241 tcgacggtca gttccatgcc gtctgaaagg gtgtggacaa aggcggtcat gcggtttcag
     3301 acggcatttt ggcggcaaac gggcctgcgc cggaaatgag cggctgctgc gtttcgatga
     3361 ggtgttcgca ttttcccatc aattcggctt cgctgccgct tgcgtgcggg atggtcggac
     3421 agatgacgac ggtgatttcc cccggatatt tcagaaagga gtttttcggc caaaattcgc
     3481 cgctgttgag ggcgacgggg acgatgtcca tctcaaacat tttcgccatg cgcgcgccgc
     3541 cgagtttgta tttgccgcgt tttccgggcg caaggcgtgt gccttcgggg aaaatggtaa
     3601 tccaatagcc ttcgtttttg cgcgccaacc cctgttttat gagctgctcg ttggcttcgc
     3661 ggcggttgtt gcggtctatg cctatggttt tgaccagttt caagccccag ccgaaaaagg
     3721 ggattttgaa caactcgcgt ttggcaacgt aaacctgcgg cggaaaaatg tcctgaaggg
     3781 cgagcgtttc ccagccgctt tggtgtttgg cgcagatgac ggcggggcgg tcggggatgt
     3841 tttccgcgcc gatgatgcgg tatttgagcc cgacgatgtg tttgagcgag aggttgagga
     3901 ttttgaccca gacccgcgcc atcttgtgcg ccccgtctcg gaaaggcgag gcgagcagca
     3961 taaagggaaa gaggaaaatc agggtggaac agagtatcag ccagtaaatc aggttgcgga
     4021 tgatgagcat ggttttgcct tgtcggaatg cggtatgttc agtcggcttg cggtgcggcg
     4081 ttttcctgca tgatgtattg tgagaaatcg agcaaggtat cgaaaacctg tgtgtgttcg
     4141 ggtaattcgt gtccgtgttg ggagagcgtt tttttgcctt ttccggtcag aaccagcgcg
     4201 ggttttccgc cgacggcatc gattgcctgc aaatcgcgca ggctgtcgcc gaccagccag
     4261 gtttccgagg cttgggcgtt gaagcgtccg atgatgtctt caatcatacc cggtttgggc
     4321 ttgcggcagt tgcagttgtc ggcatcggtg tgcgggcaga accagatgcc gttgatttcg
     4381 ccgcctgcct gacggacgag gcggtgcatt ttggcgtgca tttcggtaag gttttgaacg
     4441 gtaaaatatt tgcgcccgat gccggattgg ttggtggcaa cggcgacggt gtagcctgcc
     4501 tgcgtcagaa atgccaccgc atccatgctg ccttcgacag gtatccactc gtcaacggat
     4561 ttgacgaagt catcgcggtc ctgattgatg acgccgtcgc ggtcgagaat gatgagtttc
     4621 atcgcgtttc ctttgggttg gggcgtgtcg gggatgggat tataggacat aaatctagat
     4681 taaattctgc tacaagatgt ttctctaact ccaatatttt atcttgtgat ctgcgcttca
     4741 gtggtttaca atctaccccg acataaatag tccacggctc aagatttttt ctcactatag
     4801 cccctgctcc aacagcaacc ccatcaccaa tagtaacgcc tggtaatact gttacatttg
     4861 ttcctaaaat tgcatgtttt ccaattctaa cactagattt tttaacatta gtatatttag
     4921 atgggactgt tggattggtt aacccccccc cccccataaa atcatcgcta ccggtaataa
     4981 ttctacaccc tgcgcttaat cctgaaaaat gatccatata aaattctcca ccaccaataa
     5041 tactggtaaa tgatgatata tgaacaaaac ttcctaaaat acttttctct ccacaattaa
     5101 aaaatacaaa atcatcaatt tgagaataat ccccaattga taaattttct tttttaataa
     5161 tctttgcacc atcgtatatt ttataattca ctttttagtt cctctaaaag ggctcattgt
     5221 aacttcacca ttttcagtga ttccatctcg aataattata ctgaaatatc ggcagtgatg
     5281 cgcctgtgct gcattataat gcacgctttc gataataatt cgcaacggct caggtatgcc
     5341 gttttgagga gtggggcggg atggttgcct atgttttcct gtttttgttt gtaacggcgg
     5401 cgttggtgct gattatcagg tcgcactacc gctggacgta ttttttcgca tccgcgctgt
     5461 ttgtcttttt ggcgggcggt atgctgatgt tgacggctca gtggcaacgt gccttgaatt
     5521 tcgcttcggt ctggtttgtg gtgctgatac tgttccacag gctgaaaatc cattattaca
     5581 aacagccgct gttgatttcc gactttttgc tgattgccga ctggcggaat tgggaaacgc
     5641 tgttccatta taaggaagcg gttatcggta tggcggggct gctggctttg gcggcatatg
     5701 cggttttcgg ctggagcggt gcggattctt tggatgtgcc gtggcggtgg gcaggcgcgg
     5761 ttttgtttgc ggcggcgttc gtgtcgatgc ggcatttttc caaacatccg ggcgcggtaa
     5821 agacgtggct ggattcgctg ccggacgacg ggcgcgacgt gtttttgaac ctgccgatgt
     5881 cctgtcgggc ggtgtttttc caagtacccg tgttcgaggg cgacggggaa gcgtttgcca
     5941 ggcagatgcc gtctgaaacc cggccgtgcg gcatgtcgga tgaaaaaccc gatattgtcg
     6001 ttaccctgat ggaatcgacg ctcgatccgc actgtttcga ctttgccgcc gccaagattc
     6061 ccgatttgaa aatgttcgga cggcaggaag ataccgtatt ttcctcacct ttgcgcgtgc
     6121 atactttcgg cggcgcaacg tggaagtccg aatttgcgtt tttggcgggt gttccgtcca
     6181 cggatttcgg cgcgttggca agcggcgtgt tttactcggt cgtaccgcat ttgcagaccg
     6241 gttttgtccg caacctgcgc gaacacggtt atttttgcgt ggcactctcg ccatttacca
     6301 agggcaacta caatgccaag gcagcatatg accatttcgg ctttaatctg atgttccagc
     6361 cgcaagattt gggctatccc gcgccgatgg gcaaaaacct gtggcatatt tccagtgagg
     6421 aaatgatgca gtatgcgcgg atgattttgg aaaaacgcca tcccgatttg gaaaacgtgc
     6481 ggcagccgat gttcgtatat gtgctgacca tgaaggagca cgggccgtat cggacggata
     6541 ccgacaatgt gtttgatttg gatgcgcccg atttgaatgc gaaaaccgta tccgcgctta
     6601 acgactacat cgggcgcatt gccgatttgg acaaagcggt ggaaagtttc gaccgttatc
     6661 tgcacgaacg cggcaaaccc tttgttttcg gttatttcgg cgatcatcag gtaccgtttg
     6721 agggcgtgtc cgtcaggaag aaatgggatt acgcgcagcc ggattatgta acgcagtttg
     6781 ccgtcaggag caatattgcc ggcggattcg tacagcggca gaatttcctc gaccttgcct
     6841 ttgcaggcgg cgtgctgatg gaggcggctg gtctggaagc caaagacggc ttcatgcgtg
     6901 cgaatatggc gatgcgcggt ttgtgcggcg gagggttgga agactgcccg aactgggagt
     6961 tggtcggaaa ttaccgcaac tatctgtacg acgttttgaa aattgcccgt tagctttttg
     7021 ccgcatggct gcaacggttg cgaaaatgcc gtctgaaaac tcgttcagac ggcattttgc
     7081 ctttatcctt taaatacggg gacgagttcc atttggctca atacctgtgc ggcgatgttg
     7141 acgatgccga aaaggaagac ccaaaccatc agccacaagc cgccgtaaac tttataggtt
     7201 ttgcctgcgc cgaatttttt gcgcgaacgg tagagcagca tggcggggat gatgcctgtc
     7261 cagacggttg ccgccaggcc gacgtagccg atggcggtaa caaagccggt ggggaagagc
     7321 aggcaggaaa ttagaggcgg caggaaggtc agcgcggcgg ttttggtgcg gccggacacg
     7381 ctgtcgttcc atttgaagat gtcggcgatg tagtcgaaga gtccgagcgt tacgcctaaa
     7441 aacgaggtgg cgatcgccat ataggaaaac agggacaata ttttgtccat attgccggtt
     7501 tgggcgaatt tggacagggt ttcaatcagg acggagactt gcccttcggc ggcaatcacg
     7561 ggggcgaact cgttgcgcgg caggttgcct tggatggcgg tttgccagag gacgtaaatt
     7621 accagcgcaa tcagtgtgcc cgtccagatg gatttagcca ctttgggcgc gtcgccttta
     7681 aagtatttga gcaggctgga gacgttgccg tggaaaccga atgacgcaag gcagacgggc
     7741 agggcggtgg cgacataaat ccagtagttg gtgccggtag gggcttgggt gtcgaagagg
     7801 acgggcagtt tggcatcggc aatcaggccg ccggttgccc aaataaaggt taataccatg
     7861 ccgccgatga ggacgctggt gaatcggtcg accaagcgtg cggatgccca tacgcaaaag
     7921 gcgagaatgc cgaagaagac gagttgtccg acggtgagtg aaacattgcc gcctgccgcg
     7981 ctgcctaagc ctttggcggt caggtcgccg ccgacgaaga tataagcgta agtaagcagg
     8041 tataaaacga aggcgacggc gatgccgttg atgatgttcc agctccgtcc gagcaggtct
     8101 ttaaccatgg tgtcgaagct cgcgccgtgg gggtagtggg tgttgacttc caaaatcatc
     8161 aggccgctgg agagcatgga aaaccaggtg tacagcaaca cggccagcga gccggtaaac
     8221 catacgccgg atgtggcggt cgggttggcg agcatacctg cgccgatgac cgtgccggcg
     8281 ataatcatcg cgccgccgaa cagtgaaggg gttttggtgg gcatattttg tctttctgcc
     8341 agaaaaagcg agccgccatt atgccgtaaa gtgtaaggat ttgtaaggta tttgcgccgc
     8401 gccgcccgaa aaggctttca gacggcattg cgttccatag tataatcttg ggttttggag
     8461 tgggcggttc gtcagatggg agggaaaatg tccgacaaaa aatataatgt cgatgagggg
     8521 gaaatcgcca aattcagccg gattgccgac aaatggtggg acaagtcggg cgagttcaaa
     8581 accttgcacg acatcaatcc gctgcggctg gattatatcg acgggcacgc ggatttgtgc
     8641 ggcaaacggg ttttggacgt gggctgcggc ggcggcatct tggcggaaag tatggcacgg
     8701 cgcggcgcgg cgtttgtaaa gggtatcgac atggcggagc agtcgctgga aaccgcccgc
     8761 ctgcacgcgg ctttgaacaa tgtcgccgat atcgaatacg aatgtatccg cgtggaagac
     8821 cttgccgagg cggaaccgca ctcgttcgat gtggtaacgt gcatggaaat gatggaacac
     8881 gtccccgatc ccgccgccat cgtgcgtgct tgtgccaatc tggtcaaacc ggacggcatg
     8941 gtgttttttt ccaccatcaa taaaaacccg aaatcgtacc tgcatctgat tgtggcggcg
     9001 gaatatctgt tgaagtttgt ccccaaaggc acgcacgact ggaaaaaatt catcgcgcct
     9061 gccgagctgg cgcggatgtg ccgtcaggca ggcttggatg tggcggatac gaagggtatg
     9121 acttaccatg tgttgtcgca aacttatgcc ctgtgcgatt cgaccgatgt gaattatatg
     9181 tttgcctgcc gtccggcgtt ctgacggcgg gtttgcccgt ttttgagcaa gtgagttgat
     9241 atgtctgtct ataccagtgt ttccgatgat gaaatgcgcg gcttcctgag cggttacgat
     9301 ttgggtgaat ttgtttccct gcagggcatc gcgcagggga ttaccaacag caattatttt
     9361 ctgacgacga cttcgggacg ttatgtgctg accgtgtttg aagtgttgaa acaggaagag
     9421 ctgccgtttt ttctggagct taaccggcat ttgagtatga agggcgtggc ggttgccgcg
     9481 ccggttgcgc gcaaagacgg ccggcttgat tccgttttgg cgggcaagcc ggcttgtttg
     9541 gttgcctgcc tgaaaggttc ggataccgcg ctgccgacgg ctgagcagtg tttccatacc
     9601 ggcgcgatgt tggcgaaaat gcaccttgcc gccgccgatt tccctttgga aatggaaaac
     9661 ccgcgttaca atgcgtggtg gacggaggcg tgcgcccggc tgctgcccgt cctgtcgcaa
     9721 gacgatgccg cgctgctgtg ttccgaaatc gatgcgttaa aggacaatct cggcaatcat
     9781 ctgccttcgg gcatcatcca tgccgatttg tttaaagaca atgtgttgct tgacggcggt
     9841 caggtatcgg gtttcatcga tttctattat gcctgccggg gcaattttat gtatgacttg
     9901 gcgattgcgg tcaacgattg ggcaaggacg gcggacaata agttggatga ggcgttgaaa
     9961 aaggtgttta tcggcggtta tgagggcgtg cgccccttga gtgccgaaga aaaggcgtat
    10021 ttcccgaccg cccaacgtgc cggctgcatc cgtttttggg tgtcgcgcct gttggacttt
    10081 cattttccgc aggcgggcga gatgacgttt atcaaagacc cgaacgcgtt ccgcaacctg
    10141 ctgttgagtt tggattgagt gcgtccggcg tttgacagaa atgccgtctg aaagggtttc
    10201 agacggcatt tttatggctg attaaaacga aaatgagacg atagccgggt attttc
//
LOCUS       DAWFLT010000067        10202 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWFLT010000067 DAWFLT010000000
VERSION     DAWFLT010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10202
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.067"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(54..128)
                     /locus_tag="WGR74_001643"
     tRNA            complement(54..128)
                     /locus_tag="WGR74_001643"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(93..95),aa:Glu,seq:ttc)
     gene            complement(146..222)
                     /locus_tag="WGR74_001644"
     tRNA            complement(146..222)
                     /locus_tag="WGR74_001644"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(186..188),aa:Arg,seq:acg)
     gene            complement(332..2005)
                     /gene="recN"
                     /locus_tag="WGR74_001645"
     CDS             complement(332..2005)
                     /gene="recN"
                     /locus_tag="WGR74_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ0906019.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKSVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSREQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGWSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEECLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRSLGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDKIQRVEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(2189..2791)
                     /locus_tag="WGR74_001646"
     CDS             complement(2189..2791)
                     /locus_tag="WGR74_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ0906020.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPEAVPSDTLPN"
     gene            complement(2892..3833)
                     /gene="ylqF"
                     /locus_tag="WGR74_001647"
     CDS             complement(2892..3833)
                     /gene="ylqF"
                     /locus_tag="WGR74_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ0906021.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKIWLEHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3914..4768)
                     /gene="rapZ"
                     /locus_tag="WGR74_001648"
     CDS             complement(3914..4768)
                     /gene="rapZ"
                     /locus_tag="WGR74_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ0906022.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            complement(4749..5711)
                     /locus_tag="WGR74_001649"
     CDS             complement(4749..5711)
                     /locus_tag="WGR74_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ0906023.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQHNEDRPD"
     gene            complement(5713..6162)
                     /gene="ptsN"
                     /locus_tag="WGR74_001650"
     CDS             complement(5713..6162)
                     /gene="ptsN"
                     /locus_tag="WGR74_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ0906024.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLIFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            complement(6347..7237)
                     /gene="ubiA"
                     /locus_tag="WGR74_001651"
     CDS             complement(6347..7237)
                     /gene="ubiA"
                     /locus_tag="WGR74_001651"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ0906025.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGALLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVGKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            complement(7267..7743)
                     /locus_tag="WGR74_001652"
     CDS             complement(7267..7743)
                     /locus_tag="WGR74_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ0906026.1"
                     /translation="MEHLFGKWLPDLPAAVSDGIDLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVEARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            complement(7758..8405)
                     /gene="bioD"
                     /locus_tag="WGR74_001653"
     CDS             complement(7758..8405)
                     /gene="bioD"
                     /locus_tag="WGR74_001653"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ0906027.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGKPMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTEDLLTIDYIQQQDYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLVFNHIHDSRDAHIAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            complement(8402..9703)
                     /gene="bioA"
                     /locus_tag="WGR74_001654"
     CDS             complement(8402..9703)
                     /gene="bioA"
                     /locus_tag="WGR74_001654"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ0906028.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKSRFDDEWDGADLQPVRALFEVHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
BASE COUNT         2379 a         2791 c         2568 g         2464 t
ORIGIN      
        1 atgagaaatg agaaatgaga aatgcctccg atataatcgg aggcattttg aattggcacg
       61 cccacgggga atcgaacccc ggttaccgcc gtgaaagggc gatgtcctaa ccgctagacg
      121 atgggcgcat attgtatttt cttactggcg cacccggagc gattcgaacg cccgaccctc
      181 tggttcgtag ccagatactc tatccaactg agctacgggt gcagcacaat cacttgttca
      241 agtgattaag aaagcgtgat aatacggatt tatctgagtt ctgtctattg ttttagactt
      301 ttatttttag ttatttttta actgattgat tttaaaataa actatttttc gacgcaagtt
      361 gcagcaattc tgccgcatgt tgccgcgtcg tatcggtaat gacttctccg cccaacatac
      421 gggcaacctc ttcgacccgt tggattttat ccaatatact gatttcgctg acggtttgct
      481 ctccctcgct gtgcttgcgc acccgccagt ggttttcgcc gcaggatgcg acttgcggaa
      541 gatgggtaac ggcaagcacc tgatgttttc tgcccaacga acgtaatgcc ttgccgacca
      601 tttcagccac tccccctcca ataccggtat cgacctcatc aaaaatcagg gtgggaactt
      661 gggtatattg gctggcaaca acctgtaagg caaggctgat acgcgccaat tcgccgccgg
      721 aggcaacttt attcagcgga cggggcggat tgcctttgtt ggcggcaact tgaaattgaa
      781 cctgctctaa accgtgtgcc gtcggcgacg aaggcaacag gacgatgtcg aaacgtgcac
      841 ctttcatggc aaggtgttgc atatgctcgg tcgtttcgcc gctcaaacgc tctgccgcct
      901 gatggcgcat ggcagaaagg atgtgggcag cttcctgata ttcggcaaaa ttgtgggcaa
      961 cattatgctc gagcgcgtcc aaatcggcgg cagcttgcag gctttgcagg cactcttcga
     1021 tttctgccaa cttggcaggc aactcttcag gttcgatccg gtatttccgc gccatcccca
     1081 tcagttcgcc catacgctgt tcctgtgccg ccaattcatt gggattgatg tcgctccaac
     1141 ctgccacatc gcgcatattg gcactgattt cgcccaattc ggcttcgatg cttgccaaca
     1201 tattcaggct ctcggcaaag cgcggctcga tgttttgcag attggccaat agcttttgac
     1261 attgatagat atggcgttgg atgccgttgt cgccgtcaat cttgcttccg acttcttcgg
     1321 cagcctgcaa cagctcggca gaatgggcaa ggctgtcgtg gctttggctg agggcttccc
     1381 attcgccttg tttaatgtcc aactgattca gttcgttaaa ctgccattcc agacgctccc
     1441 gctcgataat gacggcatcg gcgtgttcct gcgcctcttg gagggctttt ttcgcattgg
     1501 cccaattttg ataaagctgc ctgacggttt ccgcctgctc cctgctgccc gcaaatgcgt
     1561 ccaacaattc gcgctgggcg gcttcctgat taagcgaatg atgggcgttt tgcccgtgga
     1621 tgtcgataag ctggctaccg acggatttga gttgcgccaa ggtagcggcc tgattgttga
     1681 taaagctgcg gcttttgcct ttggcatcga taatgcggcg gatactgagt tcttcttctc
     1741 cgtcgtttaa aagcccctgt tcatacaatt ctgcttttaa aacaggcaaa tgggaaatat
     1801 caaacaacgc cgacaactgc gcttcttttg cgccgctgcg gacttggctg taatcggctt
     1861 tatcgcccaa cagcagacca atcgcatcca aagtaatgga cttgcccgcg ccggtttctc
     1921 cggtcaatac ggtaaagccg ctttgaaaat ccagattcag attttcaaca atgacaaaat
     1981 cacgcaaaga aagtgttaga agcatcgtga ggattcctta gtaattctac cgacaaaagc
     2041 aatatcaact ttagatatac gaattataac taagattttt taaattaaaa ataagttttt
     2101 ccttaatttt tcttaatgct tgtttttatc tgcttatttg ttgcaatgcc gtctgaagca
     2161 atgtgcgttt cagacggcat ttggaatttc agttgggcag ggtatcggac ggtacggctt
     2221 ccggctcttc ttcatccgta ggcggcattt ccattaaatc gggcaaaacc agttcgccca
     2281 gttcggtcag cggcggaagt tcttccagac cgtccaagcg caaatcgctg agaaacgttg
     2341 ccgttgttgc ccacaatgcg ggttttccca atgtgtcccg atgtccgatg acttcaatcc
     2401 acccccgatc ctgcaaggtc tgcatcacgt tctgcgacac cgccacgccg cgtatgcctt
     2461 cgatgtcgcc gcgcgttacg ggctgctggt aggcgataat cgccagtgtt tccatcacgg
     2521 cgcgggagta gcgcggcgca cgctgttctt gcaggctgcc cagccgctcg aatgccgtct
     2581 gaacaatctg aaaacgccag ccctcttgcg tatgcaccag ttgcaacgcc ctatcctgcc
     2641 aacgcgtttt caactgcgcc aacacatcaa tcagtttgtc ctgcgacagt ggcggcacac
     2701 acaattcgcg catagatttt tcggtcagcg gttcggtttg ggtcagcagt gcggcttcaa
     2761 tcagcgcgtc gggagaaatt ttgtcggtca tacgggtatc cgtgctgaaa cggcatgggt
     2821 ttggatatgc cgtctgaaat cggttggagt agagagaagc tgcctgaaaa atatttttca
     2881 gacggcattc tttatgcttc cgaaggcttc tgccctttac gctctgcttt tctggcttcg
     2941 cgtatggctt tgagttcggc ttctttctga cgggcttttt tgagccaagt ttcccattgg
     3001 ttcggcgttt cgagggtgat tctgccgatt ttgccttcac ggaagtcggt gaggatgttt
     3061 tcggcggctt tttggtagtt gatccgtcca ccgctgagga ctgcgccgcg ttttttggct
     3121 atccattcga gccaaacgtt ttcgtcccag tggctgctgg ggtctttgtc ggcttggtag
     3181 cgttcttgca gcatagggag gtagtggcgg cggaggtagt ctaaaagttc gagggcgact
     3241 tcttcttcgt ccaatgcgtt gcgtccgact gcgccgccgg cggcgaggtt gtagccgctt
     3301 tcttcgacaa tgattttcgg ccacagcatt ccgggggtgt cgtacagcca gaagtcgtcg
     3361 gcaaggaaaa ggcgttgttc ggctttggtg atgccgggtt cgttgccggt tttggcggat
     3421 tttttgccta tcatgccgtt gatgagggtg gacttgccaa cgttggggat gccgcagatg
     3481 aggacgcgca ggggtttatc tatgccttgg cggtggggaa tcatggcacg acaggcttgg
     3541 gtaattttgc cgtgtgcgcc tgtttcggag gaatcgaggg cgatggcgca ggtgtcgggg
     3601 cggctgttat agtgttcgag ccagattttg gtgcgctcgg ggtcggcaag atcttgtttg
     3661 ttgaggattt taagtttagg tttgcctttg gaaagttggg caagcagggg gttttcgctg
     3721 gaggcgggca ttcgcgcatc cagcatttca atcaccatgt ctacgctttt ggcgcgctcg
     3781 gcgatggctt ttttcgcttt gttcatatgg ccgggaaacc attggattgc catgtctttc
     3841 ctttcttttc aatatttgaa atgccgtctg aaacggagga cggggtttca gacggcataa
     3901 tggtttgacg gaattagcgg tctgacaggt tttgcgcctg tctgtgccgt atcagcaatt
     3961 cataacgccc tttcaacctt cgggcgagtt tttcgacaat atagaccgaa cggtgctgtc
     4021 ctcccgtgca accgatggcg acggtaacgt agctcctgct ttcatcctcc aaacgcggta
     4081 accaatgcgt aacaaacctt tcgatgtcgt caaccatttc ctgcacaagc ggctgtccgt
     4141 ccaaataatc ccaaacgggc ttgtccatac cggtgtaagg cctcaactcg ggatcgtaat
     4201 acgggttggg caggctgcgc atatcgaaca taaaatccgc gttgttcggc acaccgtatt
     4261 tgaacccgaa ggactccaaa atcaccagca gcccggtacg ttcgaccttc agccactgcc
     4321 ggactgcatg gcggagctgt tgggcattca tcttggaagt gtcgatacaa taggcgattt
     4381 ctttaagcgg gaacagccat tcccgttctt tctttaagct ttccaacaag gtcatatcct
     4441 gattgctcag aggatgtcct cgcctggttt cggaaaaccg gcggaccaac acgctttctt
     4501 ccgcctcgac aaacaaaact tcaaccctgt gccccagtcc gcgcagagag gcaatctgtt
     4561 cccgcgcctg tccgatgtca atgccggaac gcacatcgac gctgaccgcc aattcggttt
     4621 cgtccgcacg ttcgatatga tacgacacca gcgcgggcaa catttccaaa ggcaaattgt
     4681 ccacgcagaa ataacccgaa tcttccattt ggcgcagtgc gacggacttg cccgaaccgg
     4741 acaggccgct aatcaggacg atcttcattg tgttgttcgt tttctttaag ttgcgtctga
     4801 tggcgttcca aaaattcgcg cgtactgtcc ttaccgcgca actgcaaaat gtaattgcgt
     4861 accgccgcct caaccaaaac ggcgaggttg cgtccgacgg cgacgggcag cgtaaccgaa
     4921 cggacgttga cgttgaggat ggattcggtt tcggtgcgga tgctcaaccg gtcaagctgc
     4981 ttcatatact cgtcgtccgc ctcgactaaa ttgataatga gttgcaggat ttttttgggg
     5041 cggatggaag tttcgccgaa aatatggcgg atattgagta tccccaagcc gcgcacttcc
     5101 aaaaaatcgc gcagcatagg cgaacaacgc ccttccagcg tttccgggcc gatgcggaac
     5161 agctcgaccg catcgtcggc aatcaggctg tggccgcgcg aaatcagttc caatgccaat
     5221 tcgctcttac ccaggccgga atgcccggta atcagcacgc cgatttcaaa cacatcgaga
     5281 aatacgccgt gtttgacgga cgatgccgcc aaggtgcgtt gcaggtaaat ccgcaacacg
     5341 tccatcagat aggggctttc gagtttggaa gtcagcagtg gaatatcgtt tttatgacaa
     5401 tagtcgcgca gtcccgggga aaccggcaag ccgtttgcca caatgaccaa agacatagaa
     5461 atgtcgaaca ggtcgccgaa ctgataaccc gtttcccccg attcgagacg gttcagatat
     5521 tccgattccg ccaaaccgac cacttggatt tggttggggt ggatgaaatt caggtgtccg
     5581 accaaagcga ggacgggctt gtccgcctct acgccgatac ggttgtccgc acccgaattg
     5641 ccggcggccc aggcaagttg taacttgtat tggttgtcgt caaacagtcg gcggacggaa
     5701 atactgggca tattattctt cagtcaaaat ggcgcgggct tcttccgcag aagaaaccgt
     5761 catcagcgat tcgcggatgc ttttttggga aaacttgccg gccagtttgg ataagacttc
     5821 caaatgttcg ccagttgcgt tttccggcac cagcaagata aaaatcaggg aaaccggctt
     5881 gccgtccggt gcgtcaaatc cgacgggttc gcgcgtgcgg atgaacgcgc ccgtcgcctg
     5941 cttcacgccg gcatgacggc cgtgcgggat ggcaacgccc tgccccaaac cggtcgaacc
     6001 gagtttttca cgggcaaaaa gacattcgaa aacatcagca tgggacaatg aggattcgcg
     6061 ttccaaaagc aggcccgctt cctcaaacag ccttttttta ctgcccacct ccatatccaa
     6121 aacaatatgg gacaaaggca aaatttcgcc gataaggctc ataagcttct cttttcagac
     6181 atcgcaaaac agaaagattg taccgactgc tagggcaaat ctcaatcctg catacggtac
     6241 gggctgacat aacacagcgt tttaaaaaca tattttaacg cttttcggca cagatagaaa
     6301 tgccgtccaa agcagtttac ggctcttcag acggcattgc cctgccttat ttcgcgaaaa
     6361 acgtatgggc aaaaatggcg gtaaaccaca cccaaccaat tctgttgttt gccaaaaacg
     6421 tttcaaaaca gatttgccgg acgcggcttt tgatggcggc atattggcgg tattgcagca
     6481 gcaggacgat ggggattgcc gtccaatatg cccatgccgc accgataacc gcccccaata
     6541 ctgccatcag cagggtaaag ccgccatgac acagcataac ggcggcgatg tcgtaacgcc
     6601 cgaacgtgac ggcggaggtt ttgatgccga ttttcaaatc gtcttctttg tccgccattg
     6661 cataaaccgt gtcatacgcc agagtccata acacattggc ggcaaagagt atccacgctt
     6721 gaggcggcac gtttccagca acggcggcaa acgccatcgg gataccgaag gaaaaggcaa
     6781 gcccgagata gagttgggga atcggaaaaa aacgtttggt aaacgggtaa gtcagcgcaa
     6841 gaaacagcgc gggcaggctc atcagccatg tcagatgatt cagcggaatc aggcacaatg
     6901 cggcaagcag gcacaaaaat gccgtcagca gcagcgcttc tttttttccg acccggccct
     6961 gtgcgaacgg acggtttttg gtacgctcga cagcaccgtc aaaatcgcgg tcggcaaaat
     7021 cgttgatgac gcaaccggca ctgcgcatta aaaacgtacc gattgtaaac gccgccaata
     7081 ccgccaaatc gggaatgccg tctgaagcca gccacaatgc ccagtaggtc ggccacagca
     7141 aaagcagcgc cccaatgggc ttgtccgccc gcatcaggcg caggtacaca tccaaacggt
     7201 cggacagtcg taaaaataaa ggggatttag gattcatatt gccgcacagc ttgaaaaaac
     7261 ggtattttat ccgataaagc gtttcagttc gggcagaaaa tactcggtca gcagcatttc
     7321 ctcgccgtga cgggaaaacc gagaacgccg cgcggcaaag taccgtccgc atccttcgcc
     7381 ggaaacggca aactcaaacg ccgaacgcgc cccttccaaa tcggcttgaa acagacgctc
     7441 gcccaaaggg cgcgtgccgc agtccaaaat gttttgccaa aacgccgaac ctatacggca
     7501 ctcgctcctt gcctcaacaa cagggatacc gtccagcttc aacaaaactt cgcgcaccag
     7561 cctccctccg cattccgtct ccaattcgcc cagtttcagc agttccaccg aaaatgtatg
     7621 cggcaaggcg cgcaatgcgg cggtcagcga ccgggctttc aataaccggc tcatcggcag
     7681 gtcgatgccg tctgaaacgg cggcgggcaa gtcgggcagc catttcccaa ataggtgttc
     7741 catatttttc ccaatcttta taccgcgtct gtttttgcca actccatcca ttccgcttcg
     7801 gaaaaatcgg ctttcagacg gcatttcaag tagctcaggc tgtcttgggc gatgtgtgcg
     7861 tcgcggctgt cgtggatgtg gttgaacacg aggctgtgca agcgaatgcc atattgtttg
     7921 agcgcggcga aactgagtaa agtgtggttg atactgccca gccgtccgct ggtaacgagg
     7981 atgacgggat aatcttgttg ctgaatataa tcaatggtta acaaatcttc cgtcagcgga
     8041 accatcaatc cgcccgcgcc ttcgaccaaa acgacttcgt actgcgccgc caattcttgt
     8101 gtggcggtgc ggattttgtc caagtccaaa gccctgccgt caagacgggc agcgagatga
     8161 ggcgaagctg gataactgaa gatttcaggc atggtcagcc cttgtttgtc ggcttcctgc
     8221 atgggtttac ccatgatttt acgatggacg gcgatgtcgt cggcaatgtt ttggcaaccg
     8281 gtttgcacgg gcttttgcgt aatcacgctt ttgccctgct gcaacaattg ttttgccaac
     8341 ataccggtgg cgacagtttt gccgatgtcc gtgtctatgc cgctgacgaa gtaaacgcct
     8401 ttcatttgct gtgttccttc aagatttgca cggttttgtc ggcaagtttg gtcaaaacgc
     8461 cgtctgaaat aatatacggc ggcatcagat acaccagcct gccgaacggg cgcacccaaa
     8521 tgccctgcgc cacgcaatct gcttgaaaac gcgccatatc cacgccttta tccagctcga
     8581 tcacgccgat ggcacccaaa acgcgtacgt ctttcacgcc gcgaatatcc cacgcggctt
     8641 tcagacggcc ttttaagatg ctttcaatgc ggcggatatt tgcctgccag tcttgagaca
     8701 aaagcagttt gaccgaagcg caggcaacgg cacacgccag cgggtttgcc ataaacgtcg
     8761 ggccgtgcat aaacacgccc gcttcgccgc gcgaaatcgt ttcggtaact ttttgcgaag
     8821 tgattgctgc cgccagcgtc atatagccgc cgctcaaacc cttgccgatg cacataatat
     8881 ccggcacgac ctccgcgtgt tcgcaggcaa acatcttgcc cgtgcgcccg aatccggtgg
     8941 cgatttcatc aaaaatcaaa acgatatcaa actcgtcgca caaatcgcgc aatccgcgaa
     9001 gatactgcgg atgataaaaa tacatgccgc ccgcgccctg cacgaccggc tctaaaataa
     9061 aggcggcaat atccgcatga tgcacttcaa acaaggcgcg gacaggctgc aaatccgccc
     9121 cgtcccattc atcgtcgaaa cggcttttcg gattatcgac aaaataacgc tgcggcaacg
     9181 cgctgccgaa aatatggtgc atccccgttt ccggatcgca gacggacatc gcgttccaag
     9241 tatcgccgtg atacccgcgc cgtaccgtcg caatattctg cttcgccgtc aacccccgcg
     9301 cctgctggta ttgcaccgcc atcttcaacg cgacttccac cgaaaccgaa cccgaatccg
     9361 cataaaaaat acgatccagc ccctgcggca aaatcccgac caacaatttg cccagctcca
     9421 ccgccggctc atgcgtcaaa ccgccgaaca tcacatgcga catctgtttc atctgcgtct
     9481 caaccgcctg attcaaaacg ggatgattgt aaccgtgtat cgcacaccac caagacgaca
     9541 tcccgtcaat cagccgcgtg ccgtccgcca attcgataaa caccccttct gcacgtttga
     9601 caggataaac gggaagcgga tcggtcatgg aagtataggg atggagcaga tgggtacggt
     9661 cgaaatcaag caatgatgat gtgtgttgat gttcagacgg cataagtttc tctcttttct
     9721 tattgctgca ttcaaacgca aaacgcgtat tctactccga cagaccgctt cccacacctt
     9781 tccatccgtt tcgggcgtaa aaccacgcaa caaatcgtcc gcagtataag cgcacaccgt
     9841 ttcgcattcc ccaagcccga ttggaatcag acgacccaac gcccaatatt gtttcacaca
     9901 gcctccgccc aacgcttcaa tcagtcatag acaaaacggc aatccatacg atacagatca
     9961 tagcaacagc catcgcaaca gcgttagcaa aatcagggga ctccgacata ggcgcatagc
    10021 atctaccaat gcacagctcc tcattcggct ctatgaatac catacccatc acaaaatcca
    10081 ccgccaaaac caggcacggc ttcttatact tatgatagat ttccaccatc ctgccccata
    10141 tatcccaaac attcataccg catatcccgc aggcaacaaa ttccgattga aggttacagc
    10201 cc
//
LOCUS       DAWFLT010000068         9812 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWFLT010000068 DAWFLT010000000
VERSION     DAWFLT010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.068"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            54..581
                     /locus_tag="WGR74_001655"
     CDS             54..581
                     /locus_tag="WGR74_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ0906029.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            669..1724
                     /gene="plsX"
                     /locus_tag="WGR74_001656"
     CDS             669..1724
                     /gene="plsX"
                     /locus_tag="WGR74_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ0906030.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDESPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENADGL"
     gene            1860..2111
                     /locus_tag="WGR74_001657"
     CDS             1860..2111
                     /locus_tag="WGR74_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ0906031.1"
                     /translation="MKTLEKRMKALDKQMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            2362..2553
                     /locus_tag="WGR74_001658"
     CDS             2362..2553
                     /locus_tag="WGR74_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906032.1"
                     /translation="MIQTILEDGDIEVKNLKTENYSGKAREILGDSYPIEHISKNIST
                     IQNIISEFMIQKQTPAPII"
     gene            2573..2776
                     /locus_tag="WGR74_001659"
     CDS             2573..2776
                     /locus_tag="WGR74_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906033.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPVHGNLHLKTICIPNHTPI
                     PVSVICTLSVLGNHRFFAYNSRI"
     gene            2803..3765
                     /locus_tag="WGR74_001660"
     CDS             2803..3765
                     /locus_tag="WGR74_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ0906034.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            3812..4183
                     /locus_tag="WGR74_001661"
     CDS             3812..4183
                     /locus_tag="WGR74_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ0906035.1"
                     /translation="MKRQAFFKPIACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            4209..5135
                     /gene="fabD"
                     /locus_tag="WGR74_001662"
     CDS             4209..5135
                     /gene="fabD"
                     /locus_tag="WGR74_001662"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ0906036.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFTGQAVVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFANAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKQICAESTQGEVVEAVNFN
                     SPGQVVIAGNTAAVERAMLAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQVAAFIETH"
     gene            5274..7139
                     /gene="msbA"
                     /locus_tag="WGR74_001663"
     CDS             5274..7139
                     /gene="msbA"
                     /locus_tag="WGR74_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ0906037.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKQVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAINRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDGFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDASPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            7239..8804
                     /gene="guaA"
                     /locus_tag="WGR74_001664"
     CDS             7239..8804
                     /gene="guaA"
                     /locus_tag="WGR74_001664"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ0906038.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPILGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDDTPNTLDVWMSHGDKVSKLPTGFTVIGDTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            8866..9612
                     /gene="fabG"
                     /locus_tag="WGR74_001665"
     CDS             8866..9612
                     /gene="fabG"
                     /locus_tag="WGR74_001665"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ0906039.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQAFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         2499 a         3021 c         2345 g         1947 t
ORIGIN      
        1 ggtttttgtt aatccgctat attccaaaag ccccaaccac caaggaaatt ccgatgaaac
       61 agaaaatctg gtacacctac gatgacatcc accgcgtcat caaagcattg gcagaaaaaa
      121 tccggaacgc cgacatcaaa tacgatgcca tgattgccat cggcggcggc ggctttattc
      181 cggcacgtat gctgcgctgt tttctggaaa ttccgattta tgccgtaacc accgcctatt
      241 acgacagcga caacgaagga caggttaccg aagaagtcaa aaaagtccaa tggctcgacc
      301 ccgttccaga agccctgcgg ggcaaaaacg tactcgtcgt cgatgaagtg gacgacagcc
      361 gcgtaaccat ggaattttgc ctgacagagc tgctcaaaga agacttcggt acgatcggcg
      421 tcgccgtact gcacgaaaaa atcaaagcca aagcaggcaa aatccccgaa ggcattccct
      481 atttcagcgg catcaccgta gaagactggt ggatcaacta tccgtgggac gcactcgaca
      541 tcgacgaaca caaccgcctt gccgaggccg gccgaggctg accctttcag acggcatatt
      601 ttccgaaccg atgccgtctg aagcccgcac gacccctgcc gcagaccgaa aacctaccgg
      661 agaaccctat gattacactt gccgtagatg ccatgggcgg cgaccaagga cttgccgtta
      721 ccgtacccgg cgcaaccgca ttcctccaag cacaccccga tgtccgcctg attatgaccg
      781 gcgacgaaac gcaactgcgc caagccctga ccgcggcagg cgcaccgatg gaacgcatcg
      841 acatctgcca taccacccaa gttgtcggca tggatgaatc ccctcagtcc gccctaaaga
      901 acaaaaaaga ctcctccatg cgcgttgcca tcaaccaagt taaagaaggc aaagcccaag
      961 ccgccgtatc cgcaggcaac acgggcgcac tcatggcaac cgcacgtttc gtcctcaaaa
     1021 ccattcccgg catcgaacgc cccgccatcg ccaaattcct tccttccgac accgaccacg
     1081 ttaccctcgc cctcgacctt ggcgcgaacg tcgactgcac gcccgaacag ctcgcccaat
     1141 ttgccgttat cggcagcgaa ctcgtccacg cactccatcc tcaaaaagga cagccgcgtg
     1201 tcgggctggt caacgtcggc acggaagaca tcaaaggtac ggacaccgtc aaacaaacct
     1261 acaaactgct gcaaaacagc aaactcaact ttatcggcaa catcgaaagc aacggcatcc
     1321 tgtacggcga agcagatgtc gtcgtcgccg acggctttgt cggcaacgtc atgctcaaaa
     1381 ccatcgaagg cgcggtcaaa ttcatgagcg gagccatccg ccgcgaattc caaagcaacc
     1441 tgttcaacaa acttgccgcc gttgccgccc tacccgccct caaagggctg aaaaacaaac
     1501 tcgatccgcg caaattcaac ggggccatcc tgctcgggct gcgcggcatc gtcatcaaaa
     1561 gccacggcgg cacagacgaa accggtttcc gctacgcact cgaagaagcc taccacgaag
     1621 ccaagtccgc cggcctttcc aaaatcgaac aaggcgttgc cgaacaactc gccgcactcg
     1681 aaactgccaa agccgtccaa aacgaaaatg ccgacggtct gtaacacaca cgatgccgtc
     1741 tgaacgcccc cgcccctttc agacggcatc cgcccgcacc aaacctgcgg gcgcggacgg
     1801 cgatgcgcct gtccggcact tcccaaatac ttccctgtaa aataaggagt atttgaaaaa
     1861 tgaagacatt agaaaaacgg atgaaagctc tagacaaaca gatgatgaag ttcggaaaat
     1921 cccttgaagg caggcttgat gcccgtctga ttgaatccgc attggattat attcattatt
     1981 cggaacgttt tttggctttt gaaatcctgt gtacttatat cgaagatttc gatgtccggc
     2041 tgacggaaca agaatcccgg gaaatttctt ttatcaacaa ggaatttgag atagaaagca
     2101 cgtccgatta accaataaag ccaatgggtt gataaacatg aaaacatcga cggtcgtttt
     2161 tggcggattt tttatggcag acaacggaga gcgaatccaa atccccgttt tggaaaatcc
     2221 tgacattagg gaaatcaatc acttttttcc gtatcaaatt ttgagaaaaa agccggcgtc
     2281 cttgttttca gaatcatccc cgagccggaa tttggcgata ccgaattaac tatctatttt
     2341 gaaaaaggat attatttgcc aatgattcaa acaattttag aagacgggga tattgaagtc
     2401 aaaaacctta aaacagaaaa ttacagcggg aaggcaaggg aaatcttggg agattcctac
     2461 cctattgagc atatctctaa aaatatttct acaattcaaa atataatttc tgaatttatg
     2521 atacaaaaac aaacaccggc accaataatt tgatggaaaa ccaacaatca aaatgccgtc
     2581 tgaagcatgt ttggttttca gacggcattt ccttcgctta aaacagcgta tcggcaaccc
     2641 cgccctgcct gtccacggca atctgcatct gaaaaccatc tgtatcccaa accacacccc
     2701 catccctgtt tccgtcattt gcaccctgtc cgtattgggc aatcatcggt ttttcgctta
     2761 caatagccga atctgaacca actatccaaa aaggccgttc ccatgcagta tgccaaaatt
     2821 tccggcacgg gcagctatct tcccgccaac cgcgtcagca atgacgacct tgcccaaaaa
     2881 gtggatacct cggacgagtg gattaccgcg cgcacgggca tcaaattccg ccatattgca
     2941 gccgaaaatg agaaaaccag cgatcttgcc gccgaagcag cccgccgcgc actggatgca
     3001 gccggattag acagcggcga aatcgattta attattgtgg cgacggcaac gccggatatg
     3061 cagtttcctt ctaccgccac catcgtccaa caaaaactgg gcatcaccaa cggctgcccc
     3121 gcgtttgacg tacaggcggt gtgtgcgggc tttatgtacg cgctgaccac ggcaaacgcc
     3181 tacatcaaaa gcggcatggc aaaaaacgcg ctggtcatcg gcgcggaaac tttcagccgc
     3241 atcgtcgatt ggaacgaccg cacaacctgc gtattgttcg gcgacggcgc gggcgcggtg
     3301 gttttgagcg cggcggacaa accgggcatc atccacagca aactcaaagc cgacggcaat
     3361 tatctgaaac tcttaaacgt ccccgggcaa atcgcctgcg gcaaagtttc cggttcgccg
     3421 tacatttcga tggacggtcc cggcgtgttc aagtttgccg tcaaaatgct gtccaaaatc
     3481 gccgatgacg ttatcgaaga agccggttat accgccgctc aaatcgactg gatcgtgccg
     3541 catcaggcaa accgccgcat tatcgaatcg accgcgaaac atttaggttt gagtatggac
     3601 aaagtcgtcc tgaccgtcca agaccacggc aacacatccg ccgcatcgat tccgctggct
     3661 ttggatacgg gcatccgcag cggacaaatc aaacgcggtc aaaacctgct gctcgaaggc
     3721 atcggcggcg gtttcgcgtg gggcgcggtg ctgttgcaat attgaacccg atgccgtctg
     3781 aaacaggctt tcagacggca tttcccatat catgaagcgg caggctttct tcaaaccaat
     3841 tgcgtgtgcg gcatttctgt ctgccgtttc gctgcgcctc cccgtattgg gcgcgtgtta
     3901 cgcaatattg tccctctatg cgtttgcact ttacggcatc gacaaacggc gtgccgtgcg
     3961 gggaaaacgc cgcattcccg aacaccgcct gctgctgcct gccttgttcg gcggttgggc
     4021 gggcgcatac ttgggcagca ggatattcag gcataaaacg gcgaaaaagc gttttgttgt
     4081 gctgttccgt ctgactgttt cgggcaatgt cctggcgacc tgcatcctga ttgattattt
     4141 cgttccgccc gaactttttg tcaaactcgg gcaacatctc tgacaatcca accaaacaaa
     4201 ggaataccat gtcttttgca ttcttctttc ccggacaagg ttctcaaagc ctcggcatga
     4261 tgaacggctt tacagggcag gccgtcgtca aaaacacatt tgacgaagcc tccgccatat
     4321 tggggcagga cttgtgggcg atgattaacg gcagcgatgc cgaaatcatc ggtcaaaccg
     4381 tcaataccca acccatcatg ctcgccgccg gcgttgccgt ttaccgcgcc tatttggaag
     4441 cgggtggcaa aacgcctgcc gccgttgccg gacacagcct cggcgaatat accgcgcttg
     4501 tcgccgccgg cgcattggat tttgccaacg cggtcaaact cgtgcgccta cgcgcagaac
     4561 tgatgcagtc cgccgtaccg caaggcgtgg gcgcaatggc ggcaattttg ggtttggaag
     4621 atgaacaggt aaaacaaatt tgcgccgaat ccacacaagg cgaagtggtt gaagccgtca
     4681 acttcaactc gcccggacaa gtcgtgattg caggcaatac cgccgccgtc gagcgcgcta
     4741 tgcttgccgc caaagaagcc ggcgccaaac gcgccctgcc gctgcccgtg tccgtacctt
     4801 cccattgcag cctgatgaaa cccgccgccg acaaactcgc cgaagccctg aaaaccgttg
     4861 aaatcaagca gccgcaaatc cgcgttatcc acaacgccga cgttgccgcc tacgatgatg
     4921 ccgacaaaat caaagacgcg ctcgtccgcc agctttacag ccccgtacgc tggacggaaa
     4981 ccgtcaacgc cctcgtttca gacggcattg ccgaatccgc cgaatgcggc ccgggcaaag
     5041 tgttggcggg cttggcaaaa cgcatcaaca aagccgccgc gtgcagcgca ctgaccgatg
     5101 ccggacaggt tgccgccttt atcgaaacgc actgacttcg ttccgcaaaa agcagcctgc
     5161 atgcctcagg ctgcttttca cgtccgaacg gcaaacagcc gcatatttgc gctataattg
     5221 atgacgacca aaccaccgac agcggctgcc gttgcagttc ccgccccacc gacatgatag
     5281 aaaaactgac tttcggactg tttaaaaaag aagacgcgcg cagctttatg cgcctgatgg
     5341 cgtacgtccg cccctacaaa atccgcatcg ttgccgccct gattgccatt ttcggcgttg
     5401 ccgccaccga aagctacctt gccgccttca tcgcccccct gattaaccac ggcttttccg
     5461 cacctgccgc gccgcccgag ctgtctgccg ccgccggcat catttccacc ctgcaaaact
     5521 ggcgcgaaca gtttacctat atggtttggg ggacggaaaa caaaatctgg accgtcccgc
     5581 tctttctcat cattctcgtc gtcatccgtg gcatctgccg ctttaccagc acctatctga
     5641 tgacttgggt ctccgtgatg accatcagca aaatccgcaa agatatgttt gccaaaatgc
     5701 tgaccctttc ctcccgctac catcaggaaa cgccgtccgg caccgtactg atgaatatgc
     5761 tcaacctgac cgaacagtcg gtcagcaacg ccagcgacat cttcaccgtc ctcacgcgcg
     5821 acacgatgat tgttaccggc ctgaccatcg tcctgcttta cctcaactgg cagctcagcc
     5881 ttatcgtcgt cctgatgttc cccctgctct ccctgctctc gcgctactac cgcgaccgtc
     5941 tgaaacaagt catttccgac tcgcaaaaaa gcataggcac gatgaacaac gtgattgccg
     6001 aaacccatca gggacaccgc gtcgtcaagc tgttcaacgg gcaggcgcag gcggcaaacc
     6061 ggttcgacgc gatcaaccgc accatcgtcc gcctcagcaa aaaaatcacg caggcaacgg
     6121 cggcacattc cccgttcagc gaactaatcg cctcgatcgc cctcgccgtc gtcatcttca
     6181 tcgccctgtg gcaaagccaa aacggctaca ccaccatcgg cgaatttatg gcattcatcg
     6241 tcgcgatgct gcaaatgtac gcccccatca aaagccttgc caacattagc atccctatgc
     6301 agacgatgtt cctcgccgcc gacggcgtat gtgcatttct cgacaccccg cccgaacagg
     6361 acaaaggcac gctcgcaccg cagcgtgtcg aagggcgcat cagcttccgc aacgtcgatg
     6421 tcgaataccg ttcggacggc atcaaagccc tcgacggctt caacctcgac atcagacaag
     6481 gcgaacgcgt cgccctggtc ggacgttccg gcagcggcaa atccaccgtc gtcaacctgc
     6541 tgccccgctt tgtcgaaccg tctgccggca acatctgcat agacggtatc gacatcgccg
     6601 acatcaaact cgactgcctg cgcgcccaat ttgccctcgt ctcccaagac gtattcctgt
     6661 ttgacgacac cctgtttgaa aacgtccgat atagccgtcc cgacgcgggc gaagccgaag
     6721 tcctatccgc cctccaagcc gctaacctgc aaagcctgat cgacgcatca ccgctcggac
     6781 tgcaccagcc catcggatcg aacggcagca acttatccgg cggacagcgg caacgcgttg
     6841 ccattgcccg cgccattttg aaagacgcgc cgatattatt attggacgaa gccaccagcg
     6901 cattggacaa cgaatccgaa cgcctcgtcc aacaggcgct cgaacgcctg atggaaaacc
     6961 gcaccggcat catcgtcgcc caccgcctga ccaccatcga aggggccgac cgcatcatcg
     7021 taatggacga cggcaaaatc atcgaacaag gcacacacga acaactgatg tcccaaaacg
     7081 gctactacac gatgttacgc aatatctcaa acaaagatgc cgccgtccgg acggcataaa
     7141 caaaatgccg tctgaaatgg tacaatcgcc ccgacccttt cagacggcat catatccgcc
     7201 gacccatccg attatcttca atcactgtaa aacccattat gacccaagac aaaatcctca
     7261 tccttgactt cggttcgcaa gttacccagc tcatcgcccg ccgcgtgcgc gaagcccacg
     7321 tttactgcga gctgcattcc ttcgatatgc ctttggacga aatcaaagcc ttcaacccca
     7381 aaggcatcat cctttccggc ggccctaatt ccgtttacga atccgactat caagccgata
     7441 ccggtatctt tgacttgggc attccgattt tgggcatttg ctacggcatg caatttatgg
     7501 cgcaccactt gggtggtgaa gtgcaacccg gcaaccagcg cgaattcggt tacgcgcaag
     7561 tcaaaaccat cgacagcgag ctgacacgcg gcattcaaga cgatacgcca aacacgctcg
     7621 acgtatggat gagccacggc gataaagttt ccaaactgcc taccggtttc accgtcatcg
     7681 gcgacacgcc gtcctgcccg attgccatga tggaaaacgc cgaaaaacaa ttctacggca
     7741 tccaattcca ccccgaagta acccacacca aacaaggccg cgccctgttg aaccgctttg
     7801 ttttggatat ttgcggcgca caaccgggct ggacaatgcc gaactacatc gaagaagccg
     7861 ttgccaaaat ccgcgaacaa gtcggcagcg acgaagtgat tttaggcttg tccggcggcg
     7921 tggactcttc cgtagccgcc gcgctgatcc accgcgccat cggcgaccaa ctgacctgcg
     7981 tgttcgtcga tcacggtttg ttgcgcctga acgaaggcaa aatggtgatg gatatgttcg
     8041 cccgcaactt gggtgtgaaa gtgattcacg tcgatgccga agggcagttt atggcgaaac
     8101 tcgccggcgt taccgaccct gagaaaaaac gcaaaatcat cggcgcggaa tttatcgaag
     8161 tatttgatgc cgaagagaaa aaactcacca acgccaaatg gctggcgcaa ggcacgattt
     8221 accccgacgt aatcgaatcc gcaggcgcga aaaccaaaaa agcccacgcc atcaaatccc
     8281 accacaatgt cggcggcctg cctgaaaaca tgaagctcaa gctgcttgag ccgttgcgcg
     8341 acttgtttaa agacgaagtg cgcgaactgg gcgttgcttt gggtctgccg cgcgaaatgg
     8401 tgtaccgcca tccgttcccc ggccccggtt tgggcgtgcg catcttgggc gaagtgaaaa
     8461 aagaatacgc cgacttattg cgtcaagcag acgatatttt catccaagaa ttacgcaaca
     8521 ccaccgacga aaacggcaca tcttggtacg acctgaccag ccaggcattt gccgtattcc
     8581 tgcctgtgaa atccgtcggc gtaatgggcg acggacgcac ttacgactac gtcgtcgcac
     8641 tgcgcgcagt catcaccagc gacttcatga ccgcacactg ggcagaactg ccttactcac
     8701 tgctcggacg cgtgtccaac cgcatcatca acgaagtcaa aggcatcaac cgcgtggtgt
     8761 acgatgtcag cggcaaaccg cctgccacca tcgagtggga ataaccagca aacagggctg
     8821 caccgtccgg cgcagtcttt cgattatcgg aaaaaaggaa aaaatatgag cacacaagat
     8881 ttaagcggca aaatcgcttt ggtaaccggc gcatcgcgcg gtatcggtgc agcaattgcc
     8941 gacacactgg cggcagccgg tgccaaagtc atcggtacgg cgaccagtga gagcggtgcg
     9001 gcggcgatta gcgagcggtt ggcgcaatgg ggcggcgaag gccgcgtatt aaattccgcc
     9061 gaacccgaaa ccgtcgaaaa cctgattgcc gacatcgaaa aaacgttcgg caaactcgat
     9121 attctggtca acaacgccgg catcacccgc gacaacctcc tgatgcgcat gaaagaagaa
     9181 gagtgggacg acatcatgca ggtcaacctc aaatccgtgt tccgcgcttc taaagccgtc
     9241 ttgcgcggca tgatgaagca gcgcgccggc cgtatcatca acatcacatc cgtcgtcggc
     9301 gtgatgggca atgccggtca aaccaactat gccgcggcaa aagcaggctt aatcggtttc
     9361 tccaaatcca tggcgcgcga agtcggcagc cggggcatta ccgtcaactg cgtcgccccc
     9421 ggctttatcg ataccgatat gacccgcgcc ctgccggaag aaacccgcca agcctttacc
     9481 gcccaaaccg ccttgggcag attcggcgac gcgcaagaca ttgccgatgc ggttttgttc
     9541 ctcgcttccg atcaggcgaa atacattacc ggccagacgc tgcacgtcaa cggcggcatg
     9601 ctgatgccct gacggacagc cgtgccgaca atgccgtctg aagccctttc agacggcatt
     9661 tgcattccca aacaaaatga acacacacca caccctgccc tgcccctgcg gttcaggcac
     9721 aagctgagac ctttgcaaaa ctcctttccc tcccgacagc cgaaacccaa acacaggttt
     9781 tcgtctattt tcgccccaaa tatctcctaa tt
//
LOCUS       DAWFLT010000069         9796 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWFLT010000069 DAWFLT010000000
VERSION     DAWFLT010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9796
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.069"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(11..1306)
                     /locus_tag="WGR74_001666"
     CDS             complement(11..1306)
                     /locus_tag="WGR74_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0906040.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGAQIFEQSPMTCIEP
                     HQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFQPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVKIEYSWGGECDITANLVPHFGRLAANVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQSFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1432..2319)
                     /locus_tag="WGR74_001667"
     CDS             complement(1432..2319)
                     /locus_tag="WGR74_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0906041.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2319..3284)
                     /locus_tag="WGR74_001668"
     CDS             complement(2319..3284)
                     /locus_tag="WGR74_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0906042.1"
                     /translation="MNLNKLKNKLFRRPGQRVVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNIAISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRADPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDSRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3300..4424)
                     /gene="potA"
                     /locus_tag="WGR74_001669"
     CDS             complement(3300..4424)
                     /gene="potA"
                     /locus_tag="WGR74_001669"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ0906043.1"
                     /translation="MTATTASSTKPYLQIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLGNG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4620..4727)
                     /locus_tag="WGR74_001670"
                     /pseudo
     CDS             complement(4620..4727)
                     /locus_tag="WGR74_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4806..5075)
                     /gene="rpsO"
                     /locus_tag="WGR74_001671"
     CDS             complement(4806..5075)
                     /gene="rpsO"
                     /locus_tag="WGR74_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ0906044.1"
                     /translation="MALTVEQKAQIVKDFQRQEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5298..6233)
                     /gene="secF"
                     /locus_tag="WGR74_001672"
     CDS             complement(5298..6233)
                     /gene="secF"
                     /locus_tag="WGR74_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ0906045.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVAIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIAKEAKQKEEIVV"
     gene            complement(6237..8090)
                     /gene="secD"
                     /locus_tag="WGR74_001673"
     CDS             complement(6237..8090)
                     /gene="secD"
                     /locus_tag="WGR74_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ0906046.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQFKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGSVWKPKAEMAGGKE"
     gene            complement(8302..8568)
                     /gene="yajC"
                     /locus_tag="WGR74_001674"
     CDS             complement(8302..8568)
                     /gene="yajC"
                     /locus_tag="WGR74_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ0906047.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAISAKVD"
     gene            8696..>9796
                     /locus_tag="WGR74_001675"
     CDS             8696..>9796
                     /locus_tag="WGR74_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ0906048.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRASIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKISRL
                     DNDTAISTGSLSAARFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2506 a         2609 c         2431 g         2250 t
ORIGIN      
        1 cctgcgacgc ttaacggctg tctttccact gataatattt cgagccgagg aaagaaccga
       61 gtttgcgcaa aaacgattgc aggataatat tcggctggcg caaccgctca aacggcttca
      121 gacggcattc gtcccctaaa attgcttcag caaccgccag acctgcaatg cctgttatcg
      181 ccatcccgtg tccggaataa ccttgtgcat aaaaaacatt tgcggccaaa cgtccgaaat
      241 gcgggacaag gttggcggta atgtcgcact ccccgcccca cgaatattcg attttgacat
      301 cggcaagctg cggaaaaact ttaagcatat cttggcggac aagctcggtc atacgcgcag
      361 gattgtcgat aaactcgtta tccttaccgc cgaaaagcag tctgccgtcc gcgctgaggc
      421 ggtaataatc caaaatatgg cggttgtcgc atactgccat attgttacgg ataagccctt
      481 ttgcgcgcgc ccccaagggt tcggtcgcaa taataaaggt gctgacagca atcgccttgc
      541 gttccaaagg ctggaatatc gggttcaaac ctgcataggt attgacggca tacaccagat
      601 ttttgcattc gacgctgcct tcgggcgtgt aaaccagcca accgttttga tgcggttcga
      661 tgcacgtcat cggggattgc tcgaaaatct gcgcaccggc ttcggcagcg gcacgagcga
      721 tgcccaaagt gtaattgagc ggatgcaggt gtccggataa ggggtcgaat tgtgcccctt
      781 ggtacatatc gctgtcaagc tgctgtttca actcggcttt atcccaaagt tgataatgac
      841 tcgcaccgta atgccgttgg gcgtgttcat gccactgctg caactcttcc caatgctgcg
      901 gacggacggc aaccgtggca taaccgcgct gccaatcgca atcaatggca tgtttgcgga
      961 cgcgttcgtc caccagttcg accgcctgca aagactgttg ccaaaaccat tgcgcctgct
     1021 ccaagccgac ctgtttttca atttccccca taccgcaggc gtaatcgctg ataacctgcc
     1081 cgccactccg tcccgacgcg ccgaaaccga tacgcgcggc ttccaacaca accgtttcat
     1141 gtccctgctc cgccaagggc aatgcagtgc acaaaccact caatccgccg ccgatgatac
     1201 aggtatcggt tttcagacgg cattgaagtt tcggataaac agtatgagga ttaaccgaac
     1261 tgaaataata agaaggcaga tattcttgaa aatcagggcg aatcattgtg tttgctttat
     1321 caggtgtatt ttcggacgga atgatacagg ctgtcgggcc atatcgtcca aacagaaaat
     1381 cggttgaaga aaacaggctg acccagtcat gcggtcagcc tgccttatta attaatttgc
     1441 tttctcggca gccaattttt cttggcggta ggcttctgcc gcttctcggt cacgcttggt
     1501 tgcctgcctc atcatccaat aattgacgat gatgaccaat gttccgatga tgccgattag
     1561 gatggtcgcc aagacattca tctgcggatc gagacccaac ttgattttgg agaaaatcac
     1621 ctgcggcaat gtggatgaac cggggccgga gaggaatgag gtaatcacca aatcatccaa
     1681 agacagggta atgccgagca gaaagcctga agcgatggca ggggcaatca aaggcaaagt
     1741 gatgacaaaa aagattttca gcggacgcgc gcccaaatcc attgcggctt cttcgagcga
     1801 ctggtcaagc tcaaccagac gcgaacggat aacaacggta atgtacgcca tacacagcgt
     1861 cgtatgtccg aggaagatgg tgaaaaagcc acgatcgaag tagagatgtt gtaaccattc
     1921 gctgccctgc aaaaatatct gtacctgaat aatcagcagc agcatagaca gaccggtaat
     1981 cacgtcgggc atcaccatag gtgcggaaat catgccggcg aacaaggtac tgccgcgaaa
     2041 acgtttaatc cgcgccatcg catagcctgc cagcgtgccc aaaacgacgg cggcaagcga
     2101 agacacaacg gcaatccgca gcgacagcca agcggcttcc aaaatggtgt cgttttccag
     2161 caatgcgccg taccacttgg tcgaaaagcc gccccaaacg gttaccagct tggattcgtt
     2221 aaacgaatag atgaccaaaa caaccagcgg gatatacaga aacgccagcg acagtgccaa
     2281 catcagtttc aagaaccaag ataatttgga tttctgcatt atttggctcc ttcttccaat
     2341 tcgcggtttt cataatgctg aaacagggca atcggcacga ccagcagcgc gaccatcacg
     2401 acggcgacgg cggaagccag cggccagttg ttttgatcga agaacgcctg ccacaagact
     2461 ttaccaatca tcaggttttc cgaaccgccg accagctcgg gaatgacgaa ctcgccgaca
     2521 gcagggacga aaaccagcat ggagcctgca ataatgccgg ttttcgacaa cggcagggta
     2581 atcgtcaaga acgacttgac cggccccgcg cccaagtcgg aagccgcttc gagcaggcgg
     2641 ctgtccagtt tcaccagctg cgtgtaaagc ggcagaatca taaacggcag ataggcataa
     2701 accatcacca ggtttaacga aaacgcattg tagaacaaat ctaatggctc actgataata
     2761 cccatcttaa tcaataagtt gtttacaatg ccgttgtgtc cgagcaaacc catccacgca
     2821 taaacgcgca gcaggaacga tgtccaaaaa ggcagcataa tggcaagcag caaaccatta
     2881 cggacagaag ggtcggcacg ggaaatcgca taggcggtcg gataaccgac caacaggcaa
     2941 atcactgtcg tcatcaatgc ggttttgatt gaagaccagt aggtcatcag atagatattg
     3001 ctgttttcac tgtcgccgaa cgggttaagg gtatgccaaa aattttggaa aatatcggca
     3061 tagttttgat aactgatggc gatattcaga cgacctaaat cttcgtcgat gctggtcagc
     3121 ggcgtaaacg gcggaatggc gatttcttgt tcggcaaagc tgattttcag cacgatggcg
     3181 aacggaatca gaaacagcac caaaagccaa atatacggta cggcaatcac cacacgctgc
     3241 cccgggcggc ggaacagttt gtttttcagt ttattaaggt tcattgcatt ccccttaaat
     3301 caacggaaca acggagtcgg ttggttttcc ggccagctga tatagacggt ttcgtcccaa
     3361 gtcggcggtg taatgttgcg cacataccag taaggggcgg ggacttggct tttgacgacg
     3421 cgcccgttgc cgagcttgat atggtaaatg gcgaagctgc ccaaataggc gatttctttt
     3481 accgtgcctt tcgcccagtt gtagtcgccc aaatattcgg gtttttcttt atataaatca
     3541 atatcctctg gtcgaatact aacccaaagg tcctgctcgc tcggaccacc caaaccgtga
     3601 tcgatgcgga cgtggttttc caaaccttcg cattcgataa cggcatagtc ggcatgatct
     3661 tcaatcacca caccgtcaaa gatgttggtt tcgccgataa actcggcagt gaagcggctg
     3721 ttgggatagt cgtacacgtc gctgggtgtg ccgacttgct gcaactgacc gtcagacata
     3781 atggcgatgc gggtcgccat cgtcatcgcc tcttcttggt cgtgcgtaac cataatacag
     3841 gttacgccga cttgttccag cgtattgacc aactcaagct gggtttgctg gcgcagtttt
     3901 ttgtccaatg caccgagggg ctcatccagc agtagaattt tcggacgttt tgccagactg
     3961 cgtgccaaag caatgcgctg ctgctgaccg ccggacaatt ggtgcggttt gcgtttagca
     4021 aatttggtca tctgaaccag gcggagcatt tcttcgacgc gcgcggcgat ttcgccttta
     4081 ggcattttgt cctgtttcag accgaaggca atgttttgtt ctacggtcat atgaggaaaa
     4141 agcgcgtaac tttggaacat catattgatg gggcgatcat agggtgcaag tttggtaata
     4201 tcctgaccat caaggataat ttttccctga ttgggacttt ccatacccgc cagcatacgc
     4261 agcagcgtgg attttccgct gccggaactg cccaaaagag cgaaaatttc gtgttgataa
     4321 atgtccaagt cgatgttatc gacagcgtaa ttgtcaccaa actttttcac caaaccttgg
     4381 atttgcagat aaggcttggt tgaagacgca gtggttgcgg tcataatggc aatactccaa
     4441 taaaaagacg agtaccggca aaacggattt tcgaatgggt gataaaaagc tgtttgattg
     4501 ctggcgggag tcgcaacgtc tgatgccgtc tgaaactctt gtgaagcgca cgggcagcat
     4561 gaaatggaac aagattccaa agaactttat attatattag tttatgcggt tttcgggcaa
     4621 tatagtggat taaatttaaa ccagtacagc gttgccttgc cttgccgtac tatttgtact
     4681 gtctgcggct tcgtcgcctt gtcctgattt aaatttaatc cactataaat atggatttga
     4741 gcttgtcgga aagcaacaga aaagaaaaca ccgcccattt ttctgggcgg tgtcggaaag
     4801 cgtaattatt tacgcagacc caagcgggta atcaacgcgc gatacgtatc gggctgggta
     4861 cggcgcaagt aggccagcag gcggcggcgt tggctgacca ttttcaacag gccgcgacgg
     4921 ctgtggtggt ctttggggtt ggctttgaag tggggggtca ggtcgttgat gcggaaagtc
     4981 aacagagcga cttgtacttc ggaagagccg gtgtcgcctt cttggcgttg gaaatcttta
     5041 acgatttgtg ctttttgttc tacggtcagt gccataatga aaactccaaa aatataagaa
     5101 tccccatagg gattcagaca agtttgccaa gcctgaagac aacggcaaac tccctatgcc
     5161 caagatagga caacgtggca ttatgacaca attcccgcat ttctgcacaa tattttaaga
     5221 ccgtggaagt aaccgttaaa aatgccgtct gaagcattgt ctgcttgaga cggcaatgtt
     5281 cagacggcat atgcgcttca aaccacgatt tcttccttct gtttcgcttc cttggcgata
     5341 ttgtcgcggc tcaaaccgaa cattagcaga agcgggctgg caaccaatac ggaagaataa
     5401 atgccgaaca cgatgccgat ggtcaacgcc atagaaaagc cgtgcaaggc cgcaccgccg
     5461 aacaccagca tagaaacaac catcgcctcg gtcgaaccgt gggtaatgat ggtgcggctc
     5521 atcgttgcgg taatcgcgtt gtcgatgact tccggcacgg catgtccgcg catcgccggc
     5581 ttgcggaagt tttcacggat acggtcgaag acgacgacgg attcgttcac agaatagccc
     5641 aatacggcaa ggatacccgc caagacggtc agtgaaaatt cccattggaa gaaggcaaag
     5701 cagccgagaa taatcacgat gtcgtgcata ttggcgataa tggcagatac ggcaaaacgc
     5761 cattcgaaac gcatcgacag gtaaataatg atgccgatag cgacaaaacc taaagccatc
     5821 aatccattac ttaccaattc ctcaccgact tgcgggccga taaattcgac ttggcgcaag
     5881 gtaacgtcgg gactgtcttt tttcagcaaa tccataacct gattggacaa ctgtgcggaa
     5941 gtaacacctt ctttgttcgg caggcggatc atgatgtgtt tgttcgtacc caatgcctga
     6001 acctgtacat cacctatttt cagcgtatcg aggcgttcgc gcatcttatt gacatccgca
     6061 ccctgctgat attggacttc cattaccgta ccgccggtaa attcgacaga gaaattcaga
     6121 cctctggtaa ccaaaaagaa tacggcggca ataaaggtaa ccaacgaaat aaaggtcgtc
     6181 agtttgccgt agctcataaa cggaatatcg cgtttgattt taaagagttc catagcttac
     6241 tccttgcctc ctgccatttc ggctttcggc ttccacaccg aaccaatgga aatattctgc
     6301 aatttgcgtc tgcgtccgta ccacagattg accaacgcac ggaatacgac gacggacgaa
     6361 tacatcgaag tcagaatacc caaacagtgt acgaccgcaa aaccgcgtac cgggccggaa
     6421 ccgaatacca aaagcgcgat accggcaatc agcgaagtca ggttggaatc gacaatggtc
     6481 gcccatgcgt gttggaaacc gagattgatt gcctgctgcg gcggcacgcc ggcacgcaat
     6541 tcttcgcgga tacgttcgtt aatcaagacg ttggagtcga ttgccatacc caaagtcaac
     6601 gccagcgcgg ccatacccgg taacgtcaac gttgcctgca tggcagacaa aataccgatt
     6661 aggaacagta tgttggcact caatgcaatg gtagaaaaga aacccatcag acgatagtaa
     6721 accaccatga atgcagcaac gatggcaaaa ccccataaag tcgaatggaa gcctttttcg
     6781 atgttctcct tacccaaaga cggaccgatg gtacgttctt cgacaatctg catcggcgcg
     6841 gcaagagaac cggcacgcaa cagcaaagac gtatcattgg cttcggctgt cgtcatgctt
     6901 ccggaaattt ccacgcgtcc gccggtaatg gcagtacgga taaccggcgc ggttacaacc
     6961 tcggattttc cttggtcgat caaaaccatc gccatgcgtt tgccgacatt tgcggcagtc
     7021 agttcgccaa aaatgctgcc acccgcgctg tccaagctca gactgacggc aggtgcgccc
     7081 atttggtcga aactcggttg cgcatcgttg atgttgtcgc ccgtcagctc gacctgtttg
     7141 ctgattagca gagtttcggg acgatctccg ccgcttgaaa gcagctcata accgctcggc
     7201 acgttgcctt ccaatgcctc gcgcaacttg gcaggatcgt cctccaccat acgcaattcc
     7261 aaagtcgcgg tacggccgat gatgtctttt gccttggcag tatcctgaac gcccggaagc
     7321 tgcacgacga tacggtcggc accggactgc tggatgacgg gttcggccac gcccaactcg
     7381 ttcacacggt tgtgcagggt agtgatgttc tgtttgaccg catcggaacg cactttattg
     7441 accgcctctt ccgaaagcgt caagacgata ttgctgccgt ctgaattcag cgttgcttca
     7501 ggaaacagct tgcgcaactg cggcagagcc ttttgcacat cacctgcatc ctgcaaaggg
     7561 acggtcaggc tgtttccagc ctgacgcacc gtgccgctgc ggattttttc gcggcgcagt
     7621 tcgcggcgga tgtcgcccga ataacgttca aacgttttct gcatcgccgc tttcatatcg
     7681 acctgcatgg tgaaatgcac gccgccgcgc aagtccaaac ccaaaaacat cggattggct
     7741 ttgattttcg ccatccattc ggggctgtcc gccaacaggt tgagcgcggt aatataccct
     7801 tcgcccaaag tgttttcgat gacgtcgcgc gctttaagct gcgtttctgt gtctttgaaa
     7861 cgcactttca gtgaattgtc cacaacaaac atcccgtcgg tctgaatacc tgcgtttttc
     7921 agcgcggcat ccactttgaa ttgagtctgt tcgttgatga tgatggcttg tcggttggtc
     7981 gatacctgca cggcgggtgt ttcgccgaat aggttgggca gcgaatacac tgcggcaacc
     8041 gcaatcgtga acacaatcag cagatatttc cataaaggat aacggttcat cattgttcct
     8101 taatggttgg aaccccaccc tttcggtggt gtcggaatcg ggctatttca gaagaggcaa
     8161 aaacccttcc cagccaggca agaccggaaa gcggcatcct gaatatgccg ccctgcgtgt
     8221 cggaacatgg tcaagccttc ggttggaatt caaaacaaag tgccgcattc gggcttttcc
     8281 gatgcggctt gtcggcacaa atcaatcgac ttttgcagaa atcgcattgc gttccacttc
     8341 gacctcgatt tttgtaccct gtccgatatc cacggtaaaa aactgttcgc cgactctggt
     8401 taccttaccc ttgaaacctg ccgccaagac cactttgtcg ccgactttca aggcggcaag
     8461 cattgcctga tgcgctttga atttcttttg ctgcggacgc atgatcagga agtagaacac
     8521 caccataatc aacactaaag gagcaaattg tgcaacagct tgattcataa tttatccgtt
     8581 ctttctaata tggttgaaaa tcgagagggg tatataataa cataagaccg taaacaatat
     8641 atcgggtttg ccgtccgtac cgaccgtata ccgcagcctg cccgtccaca aacccatgtc
     8701 cttgacccgc ctaatcttga aattctatgc actattgcgc ctttttttgg gcaaaaacgc
     8761 ccgcaccgca tggatttcgc atcccgcctg tgccgggcac gaacccggcg caaaccatcc
     8821 cgattcgccc gaccgcatcc tctgcatcga gcaggcattg cgccgcgcca gtatttggca
     8881 gcacctccaa accatagagg cggaagaaat cagcgatacg cgcctcgcac ttgtccactc
     8941 gagcaaatat ctgaaccgtt tggaatcttg cctgccccaa aaaggcaaga tttcccgcct
     9001 ggataacgac actgcaatca gcacaggctc gctgtctgcc gcacgctttg ccgccggttc
     9061 ggcagttcag gcagtcgaca tggtcatgaa ccgtaaagca tggcatgcct tttgcgccgc
     9121 ccgcccgccc ggacaccatg cgggcagcgg caaagccggc ggattctgcc tgctgaacaa
     9181 cgttgccgcc ggcgtcatgc atgccattgc cgaataccgc ctgaaacgca ttgccgtcat
     9241 cgatttcgat gtccactacg gcgacggtac ggcagaaata ttcaaagacg atccgcgcat
     9301 cctgtttttc aacctgtttg aaaccgacct ttttcctttc cccgaaaaca acgatatgcc
     9361 cgacggcggc aatatggtgc acctgccctt gccgccggga acgggcagcc gcacattccg
     9421 cgaagccgtc cgcagacagt ggctgccccg gcttgccgca ttcaaacccg aactggtgct
     9481 gctgtcggca ggattcgacg cacaccgtct agacgaatcg ggcaggctca acctgcacga
     9541 ggcggatttt gcctggctga cacacaaaat cattcagacg gcatcgggct gccccggcaa
     9601 aatcgtatcc gtactcgaag gcggctacac ccttgaacct ctagcacaat ctgctgccga
     9661 gcatatccgc gtgctggcag ggctgggcaa atccgatgcc gcaaccgcct atcagaaaac
     9721 actggaccca acaaaaaaac ggtttgccaa accgaaaacc ggacaggtgc gccaacctac
     9781 ccaatccgac cgttac
//
LOCUS       DAWFLT010000070         9761 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWFLT010000070 DAWFLT010000000
VERSION     DAWFLT010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9761
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.070"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(265..2955)
                     /gene="glnE"
                     /locus_tag="WGR74_001676"
     CDS             complement(265..2955)
                     /gene="glnE"
                     /locus_tag="WGR74_001676"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ0906049.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKLEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTTLDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKALVRPFVFRKYLDYGAYEAMRKLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAETGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPTSPKQRQLLAESMGFDSYSAFSDGL
                     NVHRNKVNQLFNEILSEPEEQTQDNSEWQWAWQDKPDEEGRRCRLKAHGFDAETVAAR
                     LDQIRHGHKYRHLSAHAQPRFDAVVPLFVQAAAAQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAEIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWDKLAAELS
                     DGIKACCGDTEAQMDMLRRFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTVIAAAL
                     SCAWADMPKKHRDTPQFAVIGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEILTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNIAADCGLIDKTLAGQSRTAYRFYRRQ
                     QHNTKLRDAEKTEVSDELLSHYGNVRKLWKEVFGEEAATA"
     gene            complement(3350..3541)
                     /locus_tag="WGR74_001677"
     CDS             complement(3350..3541)
                     /locus_tag="WGR74_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906050.1"
                     /translation="MGFWNGVAKAVGEGMIEAGNEHKALKMEYAEKSSEELHEIVKSD
                     GFLKIPHGRKVRLETFAIT"
     gene            complement(3678..4151)
                     /locus_tag="WGR74_001678"
     CDS             complement(3678..4151)
                     /locus_tag="WGR74_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ0906051.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLTNKSNIFELKPVLEDLASEMRGYSPKNWLYVLLSDVFHRKEEFDDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(4204..4485)
                     /locus_tag="WGR74_001679"
     CDS             complement(4204..4485)
                     /locus_tag="WGR74_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906052.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            complement(4572..4895)
                     /locus_tag="WGR74_001680"
     CDS             complement(4572..4895)
                     /locus_tag="WGR74_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906053.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            complement(5228..6235)
                     /locus_tag="WGR74_001681"
     CDS             complement(5228..6235)
                     /locus_tag="WGR74_001681"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ0906054.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTGNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPKPQPGNPQPRLFRVPE
                     YQGIINRMGFNNHGIDTMIRNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVYGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGKDAAEKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            6467..6703
                     /gene="acpP"
                     /locus_tag="WGR74_001682"
     CDS             6467..6703
                     /gene="acpP"
                     /locus_tag="WGR74_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ0906055.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            6859..8106
                     /gene="fabF"
                     /locus_tag="WGR74_001683"
     CDS             6859..8106
                     /gene="fabF"
                     /locus_tag="WGR74_001683"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ0906056.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            8461..9591
                     /locus_tag="WGR74_001684"
     CDS             8461..9591
                     /locus_tag="WGR74_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ0906057.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYHIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDSRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSRGGGGGDLERLAARDIIRFPGFVNNVSEVIKA
                     HHIFVLPSYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAE
                     KMIYFIENREAVRLMGNASYAIAKDKFDAEKVDLKLLDILKA"
BASE COUNT         2340 a         2551 c         2460 g         2410 t
ORIGIN      
        1 ccgtctaaaa agtagaatgc gctattttaa tggaaacggc ggggttttaa acggttctta
       61 tttgtttgtt ttgatttttg cttgggtaac aaatcccatc attcccacaa aaaaaatccc
      121 gtcattcccg cgaaagagga atccggtttt tgggtttcag ccattcccga taaatcgctt
      181 tagctctgcc gcaaccttaa aacggcacgg tacgaaaatg ccgtctgaaa cccagattgt
      241 caggcttcag acggcatttt ttgttcaggc ggttgccgct tcctctccga acacttcctt
      301 ccacaatttc ctgacattgc cgtaatggga cagtaattcg tcgcttactt cggttttttc
      361 cgcgtcgcgc agtttggtgt tgtgctgctg ccggcggtaa aaacggtagg cggtgcggct
      421 ttgtccggca agcgttttat caatcaaacc gcagtcggcg gcgatattca agagggcgat
      481 gttgccgtag ttgtccaaga gttgcggata ctggcgggca tgggcaagta tcagatattg
      541 gacgataaat tcaacatcga ccacgccgcc gcgtgcgtat ttgacgttgc tgtcggcagg
      601 cggatgggtg gggaacattt tttcgcgcat ttcgatgatt tcgcctgcca aggcggtttg
      661 gtcgcgttcg gcggtaagga tttcggtgcg gatgcggtcg aatgccgtct gaatttcggg
      721 tgtgccgcag atgaagcggg cgcgggtgag ggactggtgt tcccatgtcc atgcgttttc
      781 gcgctggtat ttttcgaagg cggcgatgct gtgggcgagg aagccggcgt cgccgttggg
      841 gcgcaggcgc aggtcggttt cgtagaggct gcctgcgccg gtggcggcgg aaagccagtt
      901 ggtcaggcgg cgggcgaggc ggctgtacac gtcgcctgcg tcggggtggg gatcgtcgta
      961 gagatagacg aggtcgaggt cggaggcgta gccgagttct ttgccgccga gtttgccgta
     1021 gccgatgacg gcgaattgcg gggtgtcgcg gtgttttttg ggcatatccg cccacgcgca
     1081 cgaaagggcg gcggcaatga cggtgtcggc gagggcggaa agctggtcgg agagggattc
     1141 taccgtccac aatccggcaa ggtcttggac ggcgaggcgg aagacttggg cgtgctggaa
     1201 gcggcgcagc atgtccattt gcgcttcggt gtcgccgcag caggctttga tgccgtctga
     1261 aagttcggcg gcgagtttgt cccaatcaaa cgccgtatct aataattgcg cgctgatgag
     1321 ttcgtccagc agaatcggat atttgttcag atacgccgcc acccacgagc tttggcccat
     1381 aatctctgcc agttgcgcca gggtttgcgg atgttcgttg aggaaggcga gataggcgga
     1441 tcggcggctg atgttttcga gaaaatccaa cagccgcatc aatgtatcgg tcgggttgct
     1501 ttgcgctgcc gccgcctgta cgaacagcgg cacaaccgca tcgaaacgcg gctgggcgtg
     1561 tgcggaaaga tggcggtatt tatggccgtg gcggatttgg tcgagccttg cggcgacggt
     1621 ttcggcatcg aacccgtgcg ccttcagacg gcatcgccgc ccttcttcgt cgggtttgtc
     1681 ctgccatgcc cattgccatt cgctgttgtc ttgcgtttgc tcttcgggtt cgctcaaaat
     1741 ttcgttgaac aactgattga ctttgttccg atgaacattg agaccgtctg aaaaagcgga
     1801 ataactgtcg aaacccatgc tttcggcgag cagttgccgc tgtttgggcg aggtcggcag
     1861 ggtttgggtt tgccgatcgt cccagtattg caggcggtgt tcgacatcgc gcaggaatcg
     1921 gtaggcggca agcagagttt cgacgttttc agacggcatg atcccggttt cggcaagttt
     1981 cttcagcgtt tcctgcgtgc ctttcagttg cagcgcacgc atttggccgc cgcgtatcat
     2041 ctggaaaatc tgggcgataa attcgacttc gcggatgccg cccgcgccga gtttgatgtt
     2101 gtccgccatg ccttttttgc tgacttcgct gcggatttgg cggtgcagct tacgcatcgc
     2161 ctcatacgcg ccgtaatcca gatatttgcg gaacacaaag gggcgcacca gtgctttgat
     2221 gtcgttcgga tacggcgtaa ccacgcgacc tttgcaccac gcgtagcgtt cccattctcg
     2281 cccctgtgta atcaaatatt gctccagcgc ggtttcgctc aataccaacg cgcccgaatc
     2341 gccgtccggc cgcagccgca tatcgacgcg gaacacctgc ccgtcggcgg tgatgtcgtt
     2401 cagcagcgca atcagtttct gcccgacttt ggtgaaaaac tcctgattgc cccgttcgcg
     2461 cctgccgtcg gtgtcgcccg attcgggata gacgaaaatc aaatcgatgt cggaagacac
     2521 gttcaactca tagccgcccg ccttgcccat cgccaccacg ctcaaatgct gcggcgattt
     2581 ggtataacgc ccgatcggcg tgccgtacat gtcccgataa taggcgtagg cgaaatccag
     2641 cgtggtattg acggcaaaat cggcaaacag cgtaatcgtg cgggttactt cgttcaaatc
     2701 gctgatgcgg tttatatcgc gcacgataat ctgcgacacc acataacggc gcaacccgcg
     2761 caactgccgc gccaattctt cctcgttttc ttccgcgcgg attttgtccc agtcggcaaa
     2821 ggcttggaaa tccgcttcgg tcaacacctt gtccagcatg ggcaggaaga tttcaagctt
     2881 gagtttgccg ttgtcgagct ggcgggcgag gaagagggaa tggcggcggg cggtgtcgag
     2941 gcggttgtcg gacatttcgg attccgtttg gaaggatggc aggaagatga tggcggattc
     3001 acttcaaatc aggacagggc gaggggatgc cgtaccggtt taaagtcaag ccactatatc
     3061 atagcaacct tcgatgccgt ctgaaacctt gccccgacat ccagacggat gcctgccgat
     3121 aaaaaacagc ggcaaatgcc gccgtttcct gttcacacct cgccacgctc ttttaaaata
     3181 gcataagccg agacctttgc aaaaatagtc tgttaacgaa atttgacgca taaaaatgcg
     3241 ccaaaaaatt ttcaattgcc taaaaccttc ctaatattga gcaaaaagta ggaaaaatca
     3301 gaaaagtttt gcattttgaa aatgagattg agcataaaat tttagtaacc tatgttattg
     3361 caaaggtctc aagccgcact tttctcccgt gtggaatttt taaaaaacca tcactcttga
     3421 cgatttcatg cagctcctca cttgatttct ccgcatattc cattttcaac gccttatgct
     3481 cattgccggc ttcaatcatt ccctctccca ctgcttttgc cacaccattc caaaaaccca
     3541 taatcatgtc ctccaatcgt ttcaaataaa taaggtcgtt gtttctgtca agaaacaacg
     3601 acagtttata aactgtcgga aaacaaaatg ccgtctgaag gccggataat gcttcagacg
     3661 gcatcggatt gccggtttta accctgccct ccgccgttgt tcaaatagtg ttcccagtga
     3721 gaatataacc gggcaatatt ttcttcatag gtgtgtgcag aaggaatata accgtctttg
     3781 ggcggcatat acctgacaaa tgattctatt tcttctggat aatcaaaatc tgcataaatt
     3841 ttttcaactt cccccaaagg gtcgtcaaat tcttcctttc tatggaatac atcgcttaag
     3901 aggacgtaca gccaattttt aggggaataa cctctcattt ccgaagccaa atcttccaaa
     3961 acaggcttta attcaaaaat attggatttg ttggttaaag atagtttgaa cacacgttca
     4021 tcatgatctg aaagcgtcat ttttcgggca taagctgcca catcagccca acccaagtat
     4081 ttattcccat acccccacag aatatccttc caagaaagac ggactttttg cgcggttaaa
     4141 tcaaggttca ttttttactc cgattgcctg ccccgtcatt ccgccccttc cgattgacgc
     4201 ctgttacgaa aagtcttcca aatgacggct gattgcctct tcaacagtcg gatgccaatc
     4261 cataaatgaa gcctgcaatt cctctaaaac caactcattg aataaatcat ccaaattccg
     4321 ccaatccgtt ttttcatccg gatagatgaa atttgccata acgacagtat gtttgttttg
     4381 ggaataatcc gataaggcgc aaatcatcag ataccgcccc gaatgctcca gataaaaaat
     4441 tcgtttttcc aaaggaaacc ggttattccg ttgcccgttt tccatttttc atccttttta
     4501 acgtatcggc ttccgcgtcc ttcaaattga gttacctgcc ttccgccaag caaacgcccg
     4561 cgcccctttc atcatttgaa tatccgtttg accatattta ccagataact atcttcttta
     4621 tataaatcaa tcagcaaagg cacgataccc ggctcataaa ataattctgt cgtatcagat
     4681 gtttctttta acgcggaaat tatctccaac agttcatacc cgtatcgggg ataagcaaac
     4741 aaatagggga ctgcttcccg caaacatgaa aatgcttcgt aactttgcat ttcccatata
     4801 tgttcatctt taaaatcact caaataccat tcatccaatt cactttccgg atcggatatc
     4861 aatcggataa attcatttag ttcgtcctta aacatatcaa atccttaaat ataaaatctt
     4921 ttgattatcc gccgtatcct tattttccac atcatgaatt tcaacgccgt tcatattttg
     4981 cgttttccca agccgtccgc cacagtataa aattattttt tgtatcaatt gcttatatat
     5041 ttaacaactt accgccggac ttgctttggc tgcgtatcaa gattgtccgg ttcgcggcgg
     5101 gcgtttgctt tgtttcaaac agggcggcgc gtaagggatt ttcaaggggc gtcaggcgga
     5161 aaaaggaaat gaaaatgccg tctgaacggc aaaacgtgcg ttcagacggc attttgggcg
     5221 gatcgcgtca tcgcgccaaa gccttcaaac attctttgac caatgccgga cctttgtata
     5281 tcaatccgct gtacacctgg acggcagttg cgcccaagcg gattttctct gccgcatcct
     5341 tgccttccat aatgccgcct acgccgataa tcggcagctt gccgtctatg tggtctgcca
     5401 acagcttcaa cacccgatta cttttttcat gaacgggcag cccgctcaaa ccgccctgct
     5461 cgcctgcgag cggatggctg ccgagacttg atttgtcgat ggtggtattg gtagcgatga
     5521 tgccgtccat ttcgacggat ttgacaacgt gggcgatgtc ttcgatttgt gcttcatcca
     5581 aatcgggggc gattttgacg gcgagcggaa cgtatttccc gtatacagag gcaagctgtg
     5641 cctgtttgtt tttcaaagcc tcaagcaatg cgctcaactc gtcgccacct tgcagcgcgc
     5701 ggaggttttt ggtgttgggc gaggaaatgt tgacggtaat gtagcttgcg tgcgcgtagg
     5761 ctttttcaag gcagattaaa taatcatcgg cagcgttttc gatgggtgta accgcgtttt
     5821 taccgatgtt gatgcccaat acgccactga atttactttt ttcgatgttg cgtatcatgg
     5881 tgtcgatacc gtggttgttg aaacccatgc ggttgatgat gccttgatat tcgggaacgc
     5941 ggaagaggcg cggctgcggg ttgccgggct gcggtttggg cgttaccgtg ccgatttcga
     6001 tgaaaccaaa gccgagcgcg cccaatgcgt cgatgtattc gccgtttttg tcgagtccag
     6061 cggcaagtcc gacagggttg ggcaaatcca tacccatcaa ttttacaggt ttggtacggt
     6121 tgccggttac gggaatcaga cccaatttat aaaccgtgta gagcgcgtcg agcgtgaagt
     6181 ggtgggcttt ttcggcatcg agtgcaaaca ggatgcggcg ggcaagtgga tacatgacag
     6241 ggctttctgc ttcagatgga aatgccgcca ttttaaccga aatcccgcca aacatacggc
     6301 acacaggtca aacttgaacg acaaaacccg ccgcacccaa aaatgtacaa atcggcaaat
     6361 attgatataa tagtcattag atagaaaact ctattaaaag tcggtacaat accaccgatt
     6421 tatttccctt aaacaacctg ccgcaaggca taaaggaacg actgatatgt caaacatcga
     6481 acaacaagtt aaaaaaattg ttgctgaaca actgggcgta aacgaagccg acgtgaaaaa
     6541 cgaatcttct ttccaagacg acttgggcgc ggattctttg gacaccgtgg agctggttat
     6601 ggctttggaa gaagcattcg gctgcgaaat ccccgacgaa gatgccgaaa aaatcaccac
     6661 cgtccaactg gctatcgact acatcaatgc ccacaacggc taaccggtcg tcgcccgaca
     6721 caacagcctc tgctgcaccg cgcaatagag gcttttccct tatatggcaa actgtttgaa
     6781 cccctgccgc aaattacaga cggcatctgc acgacagccg catatgcccc cattccgaca
     6841 accaacagcg aggttatcat gagtcagaga agagtagtca ttacaggctt aggtcaggtt
     6901 tcccctgtcg gcaacactgt cgcagaggct tgggacaccc tgctcgccgg caaaagcggc
     6961 atcggcgcga ttacccgctt tgacgcatcc gacatcaaca gccgtgtcgc cggcgaagtg
     7021 cgcggtttcg acatcggaca atacatcagc gcgaaagaag cgcgccggat ggacgtattc
     7081 atccactacg gcattgccgc cgcattgcag gcaatcgccg attcgggctt ggacgatgtg
     7141 gaaaacctcg acaaagaccg catcggcgtg aacatcggtt ccggcatcgg cggactgccc
     7201 agcatcgagg tcaccggcaa agccgtaatc gaaggcggcg cgcgcaaaat caaccctttc
     7261 tttatccccg gttcgctgat caacctgatt tccggacacg ttaccatcct caaaggctac
     7321 cgcggcccga gctacgggat ggtttccgcc tgtaccaccg gcgcgcacgc catcggcaat
     7381 tccgcccgac tgatcaaata cggcgacgcg gacataatgg ttgccggcgg cgcggaaggc
     7441 gcaatcagca ctttgggcgt gggcggtttt gccgcgatga aagccctctc cacccgcaac
     7501 gacgaccccg ccaccgcttc ccgtccgtgg gacaaaggcc gcgacggctt cgttatcggc
     7561 gaaggcgcgg gcatattggt gttggaagaa ttggaacacg ccaaaaaacg cggcgcgaaa
     7621 atctacgccg aaatcgtcgg cttcggcatg agttccgatg cttaccatat caccgcgccg
     7681 aacgaagaag gccccgccct tgccgttacc cgcgcgctga aagatgccgg catcaatccc
     7741 gaagacgtgg attacgtcaa cgcgcacggc acgtccaccc ccttgggcga tgccaacgaa
     7801 accaaagccc tcaaacgcgc gttcggcgaa cacgcctaca aaaccgtcgt cagctcgacc
     7861 aaatccatga ccggccacct gctcggcgcg gcgggcggcg tggaggccgt gtacagcatt
     7921 ttggcgatac acgacggcaa aatcccgccg accatcaaca tttttgaaca agacgttgaa
     7981 gccggctgcg atttggacta ctgcgccaac gaagcgcgcg acgcggaaat cgacgttgcc
     8041 atttccaact ccttcggctt cggcggcacc aacggcacgc tggtcttcaa acgcttcaaa
     8101 ggctgattcc gcaaagtcgc cgccgacatc gaaatgccgt ctgaaaccgt ttcagacggc
     8161 attttcaaaa caccccgccc gttaccggca cttcgcggca cacaaggcag ccttataata
     8221 cgcaggcaaa cccaagccct gccggattgc gggacgaaac cctgccgtag cagaccgtat
     8281 ttccaatcat atattcaagg tgtccgcctt atgtttcaac acacaggacg acacataaag
     8341 caccgcccta tgtgtcgtcc tgatttggaa ggggttacgc cccctcccaa atagagtctg
     8401 attctaccgc cccgaaggac agatgtccga gtggcggggt tcaaccgaaa aggaaacacg
     8461 atgaaaatcg tttttatcac aacagtcgca tccagcattt acggtttccg cgcccccgtc
     8521 attaaaaaat taatcggcaa aaaccatcag gtgtatgcct ttgtatcgga gttttccgat
     8581 aatgaattgg atattatcag ggaaatgggg gttacacccg ttacctaccg ttcaaaccgc
     8641 agcgggctga acccgttttc cgatataaaa tccaccttcc tcatctttaa agaactcaaa
     8701 aaaatatcgc cggatttggt tttcccttat ttcgcaaaac ccgtgatttt cggcactttt
     8761 gccgcaaaac tggcaggcgt gcccagaatc gtcgggatgc tggaaggttt gggattcgca
     8821 tttaccccgc agccggaagg cataccgtta aaaacaaaaa tcataaaggg gattttgatt
     8881 gccttgtacc acattgccct gccgatgttg gaaagcctga ttgtattaaa ccccgacgac
     8941 aaagacgaac tgacggacaa atacggcatc aaaataaaaa acatccatat tttgggcgga
     9001 atcggtctgg attcgcggca atatccttat tccgaggcgg atattcccga tgaaaaagaa
     9061 cccgtaaaat tcctctttat cggcagattt ctgaaagaaa aggggattga tgattttatt
     9121 cgggcggcgg aacaggttaa ggacaaatac cccgatacgg tttttaccgc tttgggcgca
     9181 atcgacaaat cacgcggggg gggggggggc gatttggaac ggcttgccgc ccgcgatatt
     9241 atccgtttcc ccggttttgt gaacaatgtt tccgaagtga taaaggcgca tcatatattc
     9301 gtattgccgt cttataggga aggcgttccc cgaagcactc aggaggcaat ggccgtcggc
     9361 agggcggtga ttacgacgga tgtccccgga tgcagggaaa cggtcgccga caaggtcaac
     9421 ggcttcctga tcgagccttg gaatccccgc atcttggccg aaaaaatgat ttattttatc
     9481 gaaaacaggg aagccgtccg cctgatgggg aatgcaagtt atgcgattgc caaagataaa
     9541 ttcgatgccg aaaaagtcga tttgaaattg ctcgatattt tgaaggcgta aacaaggctg
     9601 cccgcttttg agtttcggtc atttctgata aacccccgtc attcccgcca cacccccgtc
     9661 attccctcga aagcgggaat tcaggttcgt ttagtttcgg tcatttccga taaattcttg
     9721 caactttgcg tttctagatt cccactttcg tgggaatgac g
//
LOCUS       DAWFLT010000071         9728 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWFLT010000071 DAWFLT010000000
VERSION     DAWFLT010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9728
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.071"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(666..1922)
                     /locus_tag="WGR74_001685"
     CDS             complement(666..1922)
                     /locus_tag="WGR74_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ0906058.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPSDAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEEIEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLAGLKIDL
                     DTGKYLRLNSARHQTDGFFAAVLQRK"
     gene            complement(2270..2611)
                     /locus_tag="WGR74_001686"
     CDS             complement(2270..2611)
                     /locus_tag="WGR74_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906059.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2815..3672)
                     /locus_tag="WGR74_001687"
     CDS             complement(2815..3672)
                     /locus_tag="WGR74_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ0906060.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRGIYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(3733..4548)
                     /locus_tag="WGR74_001688"
     CDS             complement(3733..4548)
                     /locus_tag="WGR74_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0906061.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            4792..5577
                     /locus_tag="WGR74_001689"
     CDS             4792..5577
                     /locus_tag="WGR74_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ0906062.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLVILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDGWLESGDIVAANPKVLAQMLKIISAHV"
     gene            5836..7958
                     /locus_tag="WGR74_001690"
                     /pseudo
     CDS             5836..7958
                     /locus_tag="WGR74_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            8082..9632
                     /locus_tag="WGR74_001691"
     CDS             8082..9632
                     /locus_tag="WGR74_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ0906063.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQEAGFL
                     TVESHQYERGWFTSTETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAYIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVSAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKVHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFINSEGQFRFGTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTLPSGKIDVGGKIMFK
                     DLKKEDLNQLGLMLKKTEVDIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT         2502 a         2289 c         2533 g         2404 t
ORIGIN      
        1 gatttttgtt aatccactat aatttattga agatggctga aattcaaagt tacagaaatt
       61 tataaacaaa aaacagcaac ccgccgccgt cattcccgcg aaagcgggaa tccagtccct
      121 tgaccttaag ataatcttta attcgtcatt cccgcaaaag cgagaatccg gaagcgaaaa
      181 gtggtgggaa tgtattagaa atgactgaaa ctcaaaaaac cggattcccg cctgcgcggg
      241 aatgacgatt gatacgtttc gatatagtgg attaacaaaa atcaggacaa ggcgacgaag
      301 ccgcagacag tacaaacagt acggaaccga ttcacttggt gcttgagcac cttagagaat
      361 cgttctcttt gagctaaggc gaggcaacgc cgtactgatt tttgttaatc cactataatt
      421 gatgaaatgc cgtctgaacg ctttgaaaag ggttcggacg gcattttgtt caaaccgaga
      481 cctttgcaaa aatagtctgt taacgaaatt tgacgcataa aaatgcgcca aaaaattttc
      541 aattgcctaa aaccttccta atattgagca aaaagtagga aaaatcagaa aagctttgca
      601 ttttgaaaat gagattgagc ataaaatttt agtaacctat gttattgcaa aggtctcaaa
      661 ccggtttatt tacgttgcaa tacggcggcg aagaagccgt cggtttggtg tcgggcggag
      721 ttgaggcgca ggtatttgcc ggtatccaaa tcgattttca aaccggcaag cagttcggcg
      781 cagttgacgg gttcaaattc gggatgttcg gacaggaaac gttcgacttg cagctcgttt
      841 tcttcgggca ggatgctgca agtggcgtac accaagcggc cttgcggttt taccagtttg
      901 gaggcggcat cgaggatgct gtgttgctgt tccaaaaggt tggccacggt ttcggcggat
      961 tggcggtatt tgagatcggg attacggcgt aaagtaccca aaccggagca gggcgcatcc
     1021 actaaaacgc ggtcggcttt gcctgccagt cgggcgatac ggctgtcgtg ttcgctgctg
     1081 atgcgttcag ggtggatgtt ggtcagtccg gcgcgggtca tacgcggttt gaggttggca
     1141 aggcgttttt cggcgatatc gaaggcgtag attctgcctt tgttcgccat ttgcgcaccg
     1201 acagccaagg ttttaccgcc ggcaccggca cagaaatcga caatgatttc gccgcgtttt
     1261 gcgcccacca ataaggcaag cagctggctg ccttcgtctt gaacttccag cgtaccgtcc
     1321 aaaaacagct cgtgtttgtt aagcgcgatt ttgtttttca ggcggatgcc ccaaggcgaa
     1381 taaggcgttg cctctgcatc ggcactttcg gcttgcaaca atggcagcac tttatcgcgt
     1441 ttgcctttca aggtattgac gcgtatatcg agcggcgcag cttggttgat gctgcggccg
     1501 aaggcgagga tttcttcttc gcgccaatgc tgctgcagct gttctaccag ccattgcggc
     1561 aattctgcgg cggtattcag gccgtctgaa aactcggttt tacgggcttt caaattgccg
     1621 agaaactcta tttcttcttc atcaagcagg tctttgattt ggctgatgtt ggtgcttctg
     1681 ccgagaacca gtgcggcgag tgcggctttg cgcggctgcg cgtgcggacg gcgcagggcg
     1741 gtactgattt tttgatagtg gcgcagcgcg gcaaaggcgg tttcggcgat ttcgtggcgg
     1801 tcttggcggc cgagcttttt gtgttcgcgg aaataggcgg agaggacggc atcggaaggc
     1861 tgtttgaaag tcagcatttc agccaaaact ttggcggtat ggtcgagttg tgcggcgttc
     1921 ataatgtaat gggttctctt actgtgggtt attcataatc aatctaacgt ccttaaaata
     1981 acaatagcat ctttatcatt acttagtatt ctatccgcaa taagaaagat gtgttaaaag
     2041 tgcgcaatcg ggatttgtct aaatttctcc gttaactaca aaggcagcct gaaaaaagtt
     2101 ctattttcag gtcgcttgaa cagcatccga tataatcaca tcagcgattt cattttgtaa
     2161 tcactcccta aaacatgagg aaaaaaacaa aatttatttt cttcgttcag tatatctatc
     2221 ctaaatatgc catcactcca ttgagttttt aatttgtatt tcgctcttgt taaatgtttt
     2281 tctgtttgcc gatttctttt gctaatgtaa aaaatactct accactctca ttggttaaag
     2341 ttttcaagta ttcatcaaca tcagtaacat taataacaac tgtttcattt gtatgtttat
     2401 taaattctaa atcaataata atattcccat taaccaatat ttcatatata tcttcttctt
     2461 cagggagcat agacaaacaa taagcacttg aagctgaaaa ctcttttttc agatactgaa
     2521 gaagaaaagt atttttaatt aaaatattat taaatttatc taatgattgc ttgacttctt
     2581 tagttgtcca atttttaaca ttattcttca ttgtattcta cctggggtcg tagttattaa
     2641 attaccatac ttatttgttt ggatcgtcat aatattagtt ggcatgttgt tgttatattt
     2701 atcatatcca atatccttat taactcggat gactctttca taaattgtat gggaaatgag
     2761 atttccatca ctaagcctct caatagatat acgcgttaat gttgggaaag ggtttcagac
     2821 ggccttcata atgcgttcga tttcgtgcca aaagccgtcc ggccgacatt ccaaaacggc
     2881 aatcaagcct tggtcgatac ggcggatttc ggttgcgttc aatgtttcgg tttcggcgac
     2941 acgcgcaggc gcgaggtagg ccatacggtc gagcagatgg tagcggactt cttggcgttt
     3001 aaacccgtat tccgcccaat cttgaatata gatgccgcgc caaccgtatg gattaagcgg
     3061 tggctcaaaa gcatggaaac cgtgtggaaa aaatccgatg ccgcgcacga tggaaacggg
     3121 ttttacgtca agcggcagac cgtgttgccg caaggttcgg atgccgtctg aagtgtgcgg
     3181 cagaccgagt tgggcggtca gtttggcggc ttttcccaac agcgtgtctt tggggtcgag
     3241 tccgcgcatg tcttcgagcc tgcccgtgcc gccgccgtaa tatttgcagg tcagctcgat
     3301 atggtagggc ttgccgttaa gccttaccac aaaatccgcc gcgccttgcg tattgccgtc
     3361 cgaaccggaa accgtgaggt tgtgcgcgag cagttcggcg tgtggcgcgt tggcaaacca
     3421 aaatgccagc agttcttcgg cgtaaatgcc gaggcggtgt ccgaacgggg cgcgtcgggt
     3481 gaggtgttcc gtcagcggcg cgggatcggc atccaatgcc aaaagatagc ggaaaccgcg
     3541 ttcccccaat agttcgcgca cgcccaattc gcaaccgctt tgccacaaag gcggcgcagt
     3601 cagcagcgag gcgaggtcgc ggacgggttg gctggtgagc ttccaccata atgcgtctag
     3661 ggcgtaattc atgcttatcc gaatatcagt catgccgtct gaaaagctgg aaatgttttc
     3721 agacggcctt aatcagtctt ttttatggat acgatggctg acggtattga aaaaaccgcg
     3781 caggatatcg atgtcttcgg tttgcgtgtt ggcgcgtccg aacaggctct gcatacggcg
     3841 catcagacgc tcgccgttgc ggcggttgaa aaagccgatg tcgttcatca cgctttccat
     3901 gtgggcgacc atgcctttga tttgctcgtg ggtcgcagcg tggtcttctt gttgaagatg
     3961 ggtcatgggc gaaccggttt ggctgaagat ttcgtagcac acgacctgca cggcttgggc
     4021 gaggttgagc gagaaatagt cgggattgcc gttgatggtc atcagtcggt tgcaggcttg
     4081 gacttcttcg atgctcaagc cgaaagtctc gttgccgaaa accagtgcca ctttctcgcc
     4141 tcggtttgcg gtctgcagta attcggatac caaatcgcgc ggggtttgca gcggcgcagt
     4201 aatttcgcgg cggcggctgg tcagggcgca ggcgatggtg gtgtcggcaa gggcttcgtc
     4261 caaagaagcg gcaatggtgg cattttccaa aacgtctgcc gcgccggaag cgaggatgaa
     4321 gctttcttcc ggtaatttaa acgattgagg atgctccggg tcaaacacgg gcgggttttc
     4381 cgtcatcggc gttgccatca gatttggggc gacgatggtc agtttgtgca gacccattgt
     4441 tttcatcgcg cgcgcggcag agccgatgtt ggcgggatgg ctggtgcgcg tgaggatgat
     4501 gcggatgttg tccagataag cgggcagggc gggtttgaga gtagtcatgt tcggttttcg
     4561 gtcggaatgg aatagggtat aatgcgccgt ctttttcgtt cggacggtca aatgccgtct
     4621 gaaatgttct ttaacaacgt cggaaattta acgttgcgcc tgaagcagac acgcgtcagg
     4681 cggtatttta cccgatttgg caggatttac cccaaccaaa taaactccga ttttgccgct
     4741 ccgaaggact gtacgtccga accggcggta tgccaccctt aaggaaatct gatgaatccg
     4801 tttttgaata cagcctttaa agccgcccgc cgtgccggtc agatgatgat acgcgccgcg
     4861 ggcaatctcg atgccgtcaa aaccgacagc aaagccttca acgattttgt ttccgatgtt
     4921 gaccgcaatt ccgaaatcat cctggttgag gctttgaaag aagcctatcc gcaccacaaa
     4981 atcacttgtg aagaaagcgg ctcccacggc aaagccgcag ccgagtacga atggattatc
     5041 gatccgctcg acggcacgac caatttcctt cacggtcatc cccaatacgc catctctatg
     5101 gcgctgctgc acaaaggcgt gttgcaagaa gctttggttt acgcccccga acgcaacgac
     5161 gtatatatgg cttcgcgcgg caaaggcgcg ttgctcaacg accgccgcat ccgcgtttcc
     5221 aaccgcatcg aattgaaccg ctgcctgatc ggtaccggct tccctgttgt cgatcaaagc
     5281 atgatggaca agtatctggt gattttgaaa gatttcttgg ccaaaaccgc cggcggccgt
     5341 cgtgaaggcg cggcttcttt ggatttgtgt gctgtagcaa caggtcgttt tgacggcttt
     5401 ttcgagttta acctcaaacc gtgggacatt gccgcaggtg cattgattgt tcaggaagcg
     5461 ggcggtatcg taaccgatat gtcgggtgaa gacggctggc tggaaagcgg cgatattgtg
     5521 gctgccaatc ccaaagtgct ggcgcaaatg ttgaaaatca tttccgcaca cgtttaaatt
     5581 tcagcccgct cccgggaatg cgggaaaacg ggcgggtatg ccgtctgaag tatcgcgccg
     5641 atatttcaga cggcattttt actggcagcg ggggcaggaa ggtggaaacg gctaaatgcc
     5701 cgaaactgcc gaggatggaa actaaacgga gaaccctgtg caatttgttt atagtggatt
     5761 aacaaaaacc agtacggtgt tgcctcgcct tagctcaaag agaacgattc tctaaggtgc
     5821 tgaagcacca agtgaatggg caaagccaac gcctttaccg cgccgattac cgtcgtcaat
     5881 tacgacgaac aagccctaaa caacaccgaa gcgcgtactt tggtggatgc cgtagcgaaa
     5941 aaagacgctt ccgtttggca gttcggcggc gaaagcaaca cgctgaccgg actgtatttc
     6001 agaggttatc agcttgactc gcgccaattc agcgtcaacg gtttggcagg tatgtacggc
     6061 acgcaaggca cggccagcgt gcaagttgga tcggcacaac tgattaaagg cgcatccact
     6121 gccgtaaacg gcatggaccc tgaaggtgcg gtatccggtt ccgtcaatat cgaaaccaaa
     6181 aaagccgccg atgaaggcaa ccgcaaaatc ggtttgggct ggttcagtaa caaccgcgcc
     6241 caaggtacgt tcgacttggg ccaacgtttc ggcgaaaaca aagcattcgg cgtgcgcgcc
     6301 aacggcaaac tgcgccacgg cgacaccccg cgccacggtt acagcgaaga caacaaagaa
     6361 tttgcggtaa atgccgacta tcgcggcgaa acactgcgcg tagcgttcga ttccatctac
     6421 gcaaaacgca aaaccaacgg cggtcgcgcg cgtatgcagg acattcaaaa cgccggcgga
     6481 cgcttgtttg gcgcgcccga tggtaaaacc aacctgctgc cttcttggaa ctggcaaaat
     6541 accgccggcc aaaccaatat gcttacgttt gaatgggatg cgtttgataa tgcacagatt
     6601 acaggcggta tcggctacaa caaggcacgc tattacggca cgctgatttc cccgaccgta
     6661 tgtttgaatg ctaccagcac ttgtaccgat atgcaaaaca caaacgccag cggcaaagta
     6721 acggcacgca actatgacta caataccggt actgcgcgcc tgaccgacca atatttccgc
     6781 acattgagta tgaacctgac cgcacgcggc gaatttgaaa ccggtccggt aacacataac
     6841 tggagcaccg cgttcgaccg tgttatccgc cagcggaaaa ccataaacgg cagcggaaac
     6901 ggcaacagca agatagaagt gaaggcaaac gaaaatattg cgaatcaatt ggcatcgttc
     6961 aaagcctact atcccaattc ttgggcgaaa accgccaact tggatgccaa tatcaaagtt
     7021 aacagcctgg ctttgtccga caccttgggc ttcgttgata acaaataccg cctgactttg
     7081 ggcggacgtt tccaagcggt tgaatacacc gataagaaaa aatcacagag cggcgatgcc
     7141 aaacgtttca gcccgatgct gatggcggcg tgggtgccgc aacctgattt ggttgtgtac
     7201 ggcaactata tggaagactt ggagcctgcc gacatcaaaa ccgatgattc cggcgaaacg
     7261 acaatggcga aaccgcgtgt cagccgccag tttgaagtgg gcgtgcgtaa aaactggggc
     7321 aatttcgtaa ccacgttaaa cgcgttccaa atcaaacgcc cggctactgg cgcggtcata
     7381 caactaccaa gacaacaaaa ggggtaacaa caacgctgac ctatggcaac aattccgatt
     7441 tcgcccgcta taaggcgcaa ggcggcgcgg ccggcgacga gcaaggtatg gaacgcaatc
     7501 gcggtattga gttcaacacc tacgcgaact tgctcaacaa tactttgcgt ccgactttgg
     7561 gtctgatgta tctgcaatcg accgtgaaag agtatccaaa ttcacgcgat atgctggtta
     7621 acggcgtaca agtcgccaat ccgcgcgtaa ttgccaaagc aggtttggaa tgggatacac
     7681 cgtttgccaa aggattgact ttaaacggta atgtttcgta tttcggcaag tcttaccaag
     7741 acacgcaaaa acaatacgcc ttcccgtcct acaccttgat tgacgtaggc gcgcgctaca
     7801 aaaccaagct cggcaaaaat actttgaccg tcagcagctc ggtggaaaac ctgttcaaca
     7861 aaaactattg gcaggtacag cgcggccaat acgaccgcag cttcgccgtc gtcggtatgc
     7921 cgcgcactta ctggctgaag gcggaattgg atttctgatt tccctatatc cggaaaatgc
     7981 cgtccgaaca ggtttcagac ggcatttctt ttaaagagat atggcgggat acgcttaaat
     8041 aatgctattc tagcgtcttt gaaacctgct tgtttgaagt gatgaaaaaa cctttgattt
     8101 cggttgcggc agcattgctc ggcgttgctt tgggcacgcc ttattatttg ggtgtcaaag
     8161 ccgaagaaag cttgacgcag cagcaaaaaa tattgcagga agcgggcttc ttgaccgtcg
     8221 aatcgcacca atatgagcgc ggctggttta cctctacgga aacgacggtc atccgcttga
     8281 aaccggagtt gctgaataat gcccggaaat acctgccgga caacctgaaa acagtgttgg
     8341 aacagccggt tacgctggta aaccatatca cgcacggccc tttcgccggc ggattcggca
     8401 cgcaggcgta cattgaaacc gagttcaaat acgcgcctga aacggaaaaa gttttggaac
     8461 gcttttttgg gaaacaagcc ccggtttccc ttgccaatac cgtttatttc aacggcagcg
     8521 gcaagatgga agtcagtgtt tccgccttcg attatgaaga actgtcgggc atcaggctgc
     8581 attgggaagg tctgacgggg aaaacggttt accaaaaagg tttcaaaagc taccgcaaca
     8641 gctatgatgc ccccttgttt aaaatcaagc tggcagacaa aggcgatgcc gcgtttgaaa
     8701 aagtgcattt cgattcggaa acttcagacg gcatcaatcc gcttgctttg ggcagcagca
     8761 atctgacctt ggaaaaattc tccctagaat ggaaagaggg tgtcgattac aacgtcaagt
     8821 taaacgaact ggtcaatctt gttaccgatt tgcagattgg cgcgtttatc aatcccaacg
     8881 gcagcatcgc accttccaaa atcgaagtcg gcaaactggc tttttcaacc aagaccgggg
     8941 aatcaggcgc gtttatcaac agtgaagggc agttccgttt tggcacactg gtttacggcg
     9001 atgaaaaata cggcccgctg gatattcata tcgctgccga acacctcgat gcttctgcct
     9061 taaccgtatt gaaacgcaag tttgcacaaa tttctgccaa aaaaatgact gaggaacaaa
     9121 tccgcaatga tttgattgcc gccgtcaaag gcgaggcttc tggactgttc accaacaatc
     9181 ccgtattgga cattaaaact tttcgtttca cgctgccgtc aggaaaaatc gatgtgggcg
     9241 gaaaaatcat gtttaaagat ttgaagaagg aagatttgaa ccaattgggt ttgatgctga
     9301 agaaaaccga ggtagacatc agaatgagta ttcctcaaaa aatgttggaa gacttggcgg
     9361 tcagtcaggc aggcaatatt ttcagcgtca atgccgaaga tgaggcggaa ggcagggcaa
     9421 gtcttgacga catcaacgag accttgcgcc tgatggtgga cagtacggtt cagagtatgg
     9481 caagggaaaa atatctgact ttgaacggcg accagattga tactgccatt tctctgaaaa
     9541 acaatcagtt gaaattgaac ggtaaaacgt tgcaaaacga accggagccg gattttgatg
     9601 aaggcggtat ggtttcagag ccgcagcagt aatgggtcaa atgccgtctg aaatgttcag
     9661 gcggcatttt gttgtattgg aaactgccgt tttcaaccat tcctttagat aagccgtgtc
     9721 ggattcgt
//
LOCUS       DAWFLT010000072         9289 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWFLT010000072 DAWFLT010000000
VERSION     DAWFLT010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9289
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.072"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(48..3092)
                     /locus_tag="WGR74_001692"
     CDS             complement(48..3092)
                     /locus_tag="WGR74_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEZ0906064.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQGKGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEA
                     RISRLIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDETADIHIEEPAAPDA
                     WVVEPPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPAGFEQAQHSRIAETDHLADDVLN
                     GGWQEETAAIADDGSEGAAERSNGQYLSETEAFGHDSRAVCPFEDVPSERPSCRASDT
                     EADEGAFPSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVK
                     VKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGL
                     ELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTG
                     SGKSVGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANA
                     LNWCVNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLP
                     FIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRV
                     VEYLKQFGEPDYVDDILSGGGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGV
                     QRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3257..3937
                     /locus_tag="WGR74_001693"
     CDS             3257..3937
                     /locus_tag="WGR74_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ0906065.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAALWLAALLMLPYCLLFA
                     DSAGAYPPNALVLILPALVVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3966..4325
                     /locus_tag="WGR74_001694"
     CDS             3966..4325
                     /locus_tag="WGR74_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ0906066.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETINHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGAAVK"
     gene            complement(4991..5527)
                     /locus_tag="WGR74_001695"
     CDS             complement(4991..5527)
                     /locus_tag="WGR74_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ0906067.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(5561..5917)
                     /gene="folB"
                     /locus_tag="WGR74_001696"
     CDS             complement(5561..5917)
                     /gene="folB"
                     /locus_tag="WGR74_001696"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ0906068.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            5973..6575
                     /gene="plsY"
                     /locus_tag="WGR74_001697"
     CDS             5973..6575
                     /gene="plsY"
                     /locus_tag="WGR74_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ0906069.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPTTALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNILNLIKGKESKIGEKR"
     gene            6663..7508
                     /locus_tag="WGR74_001698"
     CDS             6663..7508
                     /locus_tag="WGR74_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ0906070.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     AVLIDPPYEEKQDYWRVTETLKSALKRFESGCYLIWYPCLSREESRKLPEELKKLVPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            7754..8728
                     /locus_tag="WGR74_001699"
     CDS             7754..8728
                     /locus_tag="WGR74_001699"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ0906071.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNARQDILGIRPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(8887..9105)
                     /locus_tag="WGR74_001700"
     CDS             complement(8887..9105)
                     /locus_tag="WGR74_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ0906072.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         2153 a         2723 c         2172 g         2241 t
ORIGIN      
        1 aatgccgtct gaaacagtct tcagacggca tttccatttg caaaaaatca agcattgtcc
       61 aaggggacga gaatcgtacg gttgccgttg tgttccggtg cggacacaat gccttccgcc
      121 tccatctggt caatcagacg cgcggcgcgg ttgtagccga tacgcaaggc gcgctgtacg
      181 cccgaaatgc tggctttgcg cgttttcagg acaacggata cggcctcgtc gtacatcgga
      241 tcggtttcgt cgtcgccgct gcgcccgatg ccgggcagct cttcgctgcc gccgccgctc
      301 aaaatatcgt caacatagtc cggttcgcca aactgcttca gatattcgac cacgcggtgc
      361 acctcttcat ccgaggcaaa cgcgccgtga acgcgctgcg gataggccgt acccggcggc
      421 aggaacagca tatcgccctg accgagcagg ttttccgcgc ccatttggtc gagaatcgtg
      481 cggctgtcga ttttgctgga tacttgaaac gcgatacgcg tcgggatgtt tgccttaatc
      541 agacccgtga tgacatcgac gctggggcgt tgcgtggcaa ggataagatg gatgcctgcc
      601 gcgcgggctt tttgggcgag gcgggcaatc agttcctcga ttttcttgcc tgccgtcatc
      661 atcaaatcgg caaactcatc gaccacgacc acgataaacg gcagtttttc caaaggttcg
      721 ggatcgtcgg gcgtgaggct gaacggattg ccgatttttt ctccccttgc tgcggcttcg
      781 gcgatttttt gattgaagcc cgcaagatta cgcacgccca taaagctcat caggcggtag
      841 cgtttttcca tttcgttaac acaccagttc agcgcgtttg ccgccagctt catatcggta
      901 acgacagggg cgagcaggtg cgggatgcct tcgtaaatgc tcaattccag cattttcgga
      961 tcgatcataa tcatacgcac gtcttccggc gcggctttga aaagcataga cagaatcatc
     1021 gcgttgacac ccaccgattt gcccgaaccg gtcgtgccgg caaccagcaa atgcggtgct
     1081 ttgcccaagt cggttacaac gggctgtccg gtgatgtcct gaccgagcgc gagcgtcagc
     1141 ttggatttgg attcggcaaa ctcgggcgaa ttgaagattt cgctcaggcg tatcatttgg
     1201 cgtttcgggt tcggaagttc caaacccata caggttttgc cggggatggt ttcgacaacg
     1261 cggatggaag ccacgccgag cgaacgcgcc aaatcttttt ccagattcag aacggaattg
     1321 ccgcgcacgc cgacatcggg ttcgatttca taacgcgtaa ttacggggcc ggaataagaa
     1381 tcgacaacct tgaccttgac tttgaactcc gccaattttt cttcgatggt gatgctgttt
     1441 tccagcagtt cttcttcggt ttgcgtcgcc tcgggattga acagcggagg cagaagcagg
     1501 tcggttgtcg gcaggtgttc ggataccgca ccggtttctt ccgatgggaa cgccccttca
     1561 tccgcttccg tatccgatgc ccggcaggac gggcgttcag acggcacatc ttcaaacgga
     1621 caaaccgccc gactgtcatg cccgaacgct tcggtttccg acagatattg cccgtttgac
     1681 cgctctgccg caccttcact gccgtcatct gcaatagcgg cggtttcctc ctgccaacct
     1741 ccattcaaaa catcatcggc aagatggtcg gtttcggcaa tgcggctgtg ttgcgcctgc
     1801 tcgaatcctg ccggcggttc ataggtacgg ttgtagattt ccgatacggg aggcggcggc
     1861 ggaatatcga ttgcgggcat gggaactttc ggcacttcgg gtggttcgac cacccaagca
     1921 tcgggcgcgg caggttcttc aatatggata tcggcagttt catcataaac cggaccgccg
     1981 tatcccacag acgaaacagt ttccgtgaaa acattttcgg tttcagacgg catttcgaca
     2041 tcccgtttcc cgacaaccgt ccgactttcc ggaatcaggc gcgaaatccg cgcctccgca
     2101 gaaaccgttg ccttgttcgg ttctttcaaa ttgacggaaa cgcggcggcg ggcatcgcgg
     2161 acggctgtgc cgtctgaaac ggactgccct tgggaaacat ccggggattt tgcctccgcc
     2221 tgccctttcc cctgccctgc atgacggcgg aacggcgcat tccgtgcatt ttcggctttg
     2281 actgcagaaa aggcagacgg atgctgcttc tcaaaatacg gctcaaatcc gtaatccgca
     2341 gactcggaaa acgcttcttt gtccgcatcg aacatacggg aatacgtgcg ttgaagcaca
     2401 gggtcgtcca aaccgataat ctgcagccct tccatcgggg tatcggacac gcggactttg
     2461 gggaccgctg tttcgttttt ctcaaatgca tcgatatagc gttttttcgt ttcccttaaa
     2521 gccgcgctgt ttaaagcacg cgttgcttct tccaaagtga tttccttaaa aaccggacgg
     2581 acgggcgaaa tttcgctttc agacggcatc aacccttcag caatactccg gtcgaatggg
     2641 atgcggcggt tgtcgattac ggcagttgca atgtcttccg tatcggcagc ctcttcctcc
     2701 gcagcttctg cttcttccgt ttccgcttct tccgtcccgt ttccactgtc tgaatatccg
     2761 tcggatgcgg tcggaacatc cgcacgattt gcagaagcag gcggaacggg cctgtccggt
     2821 tcggattcgt gcgccagata gtttcggcag aaacggacga caccgacaaa cagccaatac
     2881 actgccgttt tgacggcatg gaaaagcatc agggcaagtt cgggaaaatc gggcataccg
     2941 tctttgattt caggcagctc cgcctgtttt tcccctttct tttcctgcca cgcagaaacc
     3001 tcgcgcatcc actcgcgttc ggattgtgcg cggacaaaaa acaagccggc aagcgcaagc
     3061 aacaaaataa cgatcaaaac tatccaaaac atatgccgtt tcccgcaaga caaacgatta
     3121 aacgggttta ttttaacgtg tttccatccc aaaaaaaaca accggtcggc aattctgccg
     3181 aaaccgattc ggctttcggt ttgcccccgc cgacagtata atagccgtcc cttcccttaa
     3241 tctttgcggc attgccatga ttttccaaac agtctggttt tcagatatgg tactgtcggt
     3301 ttcgtggatt gtccttatcc tgatactggc agcttccgcg ccgtccgcat tccgctcgct
     3361 tgcccgctac cgttccgccc ttccactgtg taccgtaatt ttttccgcag catggtgtct
     3421 gaacgcctct gccggcggcg gacagcttgc acagatgaac taccacctgc tcgccgtcaa
     3481 tctggttgca ctgatggtgg gcacttccgc cgccctttgg cttgccgcgc tgctgatgct
     3541 gccctactgc ctgctgtttg ccgattctgc cggcgcatat ccgccgaacg ctttggtgct
     3601 gattctgccc gcgctggtcg tcaaccgcct gtcgcgtatg ctggtcaacc gcctgccgcc
     3661 caatattttc atctttattt tcgtcaacgg ctttctcgct tccgccgccg gcattctgtt
     3721 gaccgggctg gtgctgaccg gtattttgga tgctgcaaac gcttttccgt ccgaaatatt
     3781 gtggacgacc gccctgcccg tctttattct gctggcgtgg gcggaagcct tcctcagcgg
     3841 catttcaact gccatatttg ttgcactgaa gccccattgg atcaacactt tcgacgacaa
     3901 ccgctatctg aagtccgacc gcggcatatg gcggtagcct tcagacggca taggcagaca
     3961 aaaccatgct ttccaacatc atcccccttt ccatcggtgc ggcactcggt gcaacggcgc
     4021 gttggctgct caacctcgcc gttcctgccg ccatgtctcc cgccacgggc aacctgtttg
     4081 ccaactggac gggcgcgctg ctcatcggca tctttgccga aaccatcaat cacccgcaat
     4141 ggaagctgct gctgattacc ggttttctcg gcagcctgac cacgctttcc ggattttcac
     4201 tggaaaccgt aaccctgctc caatcgaatc gtcctgcttc agcacttgcc aatattttcc
     4261 tacacacggc aggttcgctg ctgctgactt ggctcgggct gaagataggg gcggcagtca
     4321 aataaccgca acaaattgcc tccctttgtt ttgacataat ctataaatta gcttcgcttg
     4381 tttaaatact ggctgtgcca attatagtgg attaacaaaa atcaggacaa ggcgacgaag
     4441 ccgcagacag tacagatagt acggaaccga ttcacttggt gcttcagcac cttagagaat
     4501 cgttctcttt gagctaaggc gaggcaacgc cgtactggtt tttgttaatc cactataccg
     4561 ttctcaaatt caaattgggc ttttaggtct ataacaaaga aaaagccttg gtaggataaa
     4621 cctaccaagg ctttttctta aaaaaacgct ctattaccgt gcctaaaaat gcaaaaaagt
     4681 aaccgtcata acccttttgc caaaatcaat atcatcatcc gatgaatgaa ttgacggtat
     4741 ataaggacgg tatataagaa agaattaata agctcataaa ccggtaagaa aaatcagtaa
     4801 caaagatatt cattattaac cattttaaaa attatcatgt tcgtttgggt aattattata
     4861 cccttttccc tcttattact aaagcacgaa tgactttaaa ggacgcgccc agagacaagc
     4921 cgaaaaggca tatctcatcc gccaaacaaa atgcccaccg acttactccg gcaggcagtt
     4981 caacaccctg tcaatccttc atcagccagt attgcaaacc gattaatgtc ttgccgtctt
     5041 taatttcatc gtttgccaat gcctgacgga cttcttcttt cgacatcaat acggtttccg
     5101 taatctcgtc ttcgtcattg gcaagcgtac tgcccaaacg cacgccttcc gcctcgaaca
     5161 gatacatttt ttcgttgcaa aaaccgaccg ccgtataaaa actgtacagc aggcgcacgc
     5221 tgtcggcaac ataaggtgtt tcctccgcca attctcgcag cgcacacgct gccatatcct
     5281 cgcctgccac atccagcttg cccgcaggaa gttccaaggt cgcctgattt gccgcataac
     5341 gccactgccg caccaaaacg accttttcat catccgttac cgccaacacg cacgccgcgc
     5401 ccggatgacg gatgacgata cgctgccctt cattgccgtt gggcaacctg accttatccc
     5461 tgctgatgct gacaaaaccg ccctcgtaaa tggtttcgcc gcctaatttt acttccctca
     5521 aatccacttc atatctcctt tccgtttcct attctgccgt tcaatcttca cgcttgccgc
     5581 gctcgatttc cacgccaacc tcgcgcacgc cttccaaaat acccggcttg acgattttca
     5641 cgcgtaccca caccgcgccg aaatatccca aaaccaaatc ggcaatatat tccgccaacg
     5701 cttccaaaag caggaaatcc tgttctttca gatgtcggcg cagcgtttcg catacctcgg
     5761 cataatgcac cgtattggca atatcgtcgt ccgaacccgc tttctcggga acgccgatgt
     5821 ccaaatcgac aatcagggtc tgcaaccgtt cgcgctccca gccgtacacg ccgataagcg
     5881 tatccgcctt gatgccgtgc aaaaagattt tatccataat ccgccccgtt ttttgctaaa
     5941 ataaccgcac cattctaagt aaagtaccgc aaatgttcaa cataccggct gttgccgttt
     6001 cctatctgat cggctcactt tctttcgccg tcatcgtgtc caaatattac ggcatggacg
     6061 acccgcgcac ctacggatcg ggcaatccgg gggcaaccaa tgttttacgc agcggcaaaa
     6121 aaaaggcggc cgcgctgacg ctcttgggcg atgccgccaa aggtttggtt gccgttttgc
     6181 ttgcacgcgt gcttcaagaa ccgctcggtt tatccgacag cgcaatcgcg gccgtcgcac
     6241 tcgccgcgct ggtcgggcat atgtggccgg tgtttttcgg atttaaaggc ggcaaaggcg
     6301 tggcaacggc attgggcgtg cttctggcac tctctcccac aactgccttg gtctgcgcgt
     6361 tgatttggct tgtgatggca ttcggcttca aggtgtcctc ccttgccgca ttaaccgcca
     6421 caatcgccgc cccccttgcc gcactgtttt ttatgccgca tacttcttgg attttcgcaa
     6481 ccctcgcaat cgccatattg gtgttgctcc gccataagag caacatcctc aacctgatta
     6541 aaggcaaaga aagcaaaatc ggcgaaaaac gctgattcgg catttgtttt ccattacgtc
     6601 atgccgtctg aaaccatgtt cccgcgttca gacagcattt ccgcacaaac ggctttcata
     6661 ccatgctcag ttacagacac gcattccacg ccggcaacca cgccgatatg ctcaaacact
     6721 ttaccttgtt tttagtgctg caatatttca accgcaaaga caagccctac tggtacatcg
     6781 acacgcacgg cggcgcgggt gtgtacaatc tcgaaggcag cgaggcgcag aaagtcggcg
     6841 aataccggca aggcatcgcc ctgctccgac aggcgcaaaa cctgcctgcc gaactctctg
     6901 actttgccgc gcacatacaa aaaatcctgc cctcgcccga actttactgc ggttctccgt
     6961 ggctggcaca atcgctgacc cgcgtcggcg acaaattacg cctgtttgag ctgcacccca
     7021 ccgactttgt ccatctgcaa aacaatatgg gcgaagcagg attgggcaaa cgcggacaag
     7081 tgttgcgcga agacggttac aaagggctga tttccctgct gccgcccccg ccgcgccgtg
     7141 ccgccgtcct catcgacccg ccttacgagg aaaaacagga ctactggcgt gtaacggaaa
     7201 cgctgaaatc cgctttaaaa cggtttgagt ccggctgtta tctcatctgg tatccctgcc
     7261 tcagccgcga agaaagccgc aagctgcccg aagaactgaa aaaactcgtt cccgacaact
     7321 accttcacgc cgaactgcac gtccacgcgc cgaaagccga cggtttcggt atgcacggca
     7381 gcggaatgtt cgtcatcaat ccgccctatc tgttggcgga acaattggcg gcaaacctcc
     7441 ccgccctgac gcggctgctg gcgcaagacg aaggcgcgcg ctaccttttg gacagcaaaa
     7501 tccgctgacc ccgatgccgt ctgaaaccct tcagacaaca tctcccgtca aataaacaaa
     7561 accggacgat ataccggcag tcggcacttt taagaataaa tatcccctca tccgcctgcc
     7621 gcgttccgac ttgccccatg ccgttttttt acactacaat cccctagact atcctgcaaa
     7681 acgcaccaaa ggaatacgcc acaataacct gccgcctttc agacggcaca aacctttccg
     7741 aaacgagcaa actatggaca cactgacccg attcctcccc gaacacctgc aacaaaacca
     7801 gctgcccgaa gcactcggcg gcgttttact gtccgttgtt tccgcctgca ccgaaatcaa
     7861 cgccaaagtc cgcctcggcg cactggccgg cgtattgggt atggcgggca cgggcaatat
     7921 tcagggcgaa gaccaaaaaa aactggatgt tatcgccaac aacatcatga ttgacacact
     7981 caaagccaac cctgccgttg ccggtttggc aagcgaggaa gaagactctt tcgtaagtgc
     8041 cggtgaaaac ggacgctatc tcgtcctatt cgatccttta gacggatcgt ccaatattga
     8101 tgtcaacatt tccgtcggta cgattttctc catccttgaa aaacccgaag gcgcattggc
     8161 aaccgaatca ttccttcaaa cgggcagaca gcagcttgcc gctggttacg ttctttacgg
     8221 gcctcaaacc cagctcgtat tcacattcgg acacggcgta tatatgttca cgctcaatgc
     8281 cgaaaacgaa tttgtgctga caaaagaaaa accgaaagta cccgaaagta ccaaagaatt
     8341 tgccatcaat atgtccaacc gccgccactg gctgcccccc gtccaacaat acatcgacga
     8401 gctcttggcg ggcgaaaccg gcacgcgcgg caaaaactac aatatgcgct gggtagccag
     8461 catggttgcc gaaatccacc gcatactgat gcgcgggggc gtgttcatgt atccgcaaga
     8521 caaacgcgac ccgtccaaac ccggcaaact gcgcctgatg tatgaagcca atcctatggc
     8581 cctgatactc gaacaagccg gcgcatcggc aagcaacgcc cgtcaggaca tactcggcat
     8641 ccgtcccgaa agcctgcacc agcgcgtcgc cgtcattatg ggcagcagcg aagaagtgga
     8701 ttacctcaac cgcctgcatt caaaataaaa ggcagcctga ccgataaaga aaggcggcag
     8761 cgcgcatccg acgctgccgc cgatatttcg tttatagcct taaaaagata gaaaaccacc
     8821 tttccgaacg gcaaaaatgc cgtctgaaaa gttttcagac ggcattttaa aaattaaagc
     8881 ccgaatttat ttgcccgctg ctttttgcag gatggcgtac aactcgtctt tcagtttcaa
     8941 tttggctttt ttcagctcat cgatggtttc cgcaccgctg gtaaccggat tgttgaccag
     9001 accggtaatt ttatcgtcca gctcgttgtg ttcgtcgaac agacgggcga agcgagaatt
     9061 ttcctgtttc aatttggaaa tcaaatcacg gtattctggg aacatatcgc tttcctctct
     9121 tggtttacgg ttaaaaagtc aaattgaatc tttacattaa ttataacaaa gataatattt
     9181 ttatccagtg ttttatcgga tacggcaaaa ttttctttta acaccgccgt ccggttgata
     9241 aaaacgttga caatatcacg ctttacgcat aaattttcag cctttccta
//
LOCUS       DAWFLT010000073         9176 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWFLT010000073 DAWFLT010000000
VERSION     DAWFLT010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9176
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.073"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(254..2857)
                     /gene="pepN"
                     /locus_tag="WGR74_001701"
     CDS             complement(254..2857)
                     /gene="pepN"
                     /locus_tag="WGR74_001701"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ0906073.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGVAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GECSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDCFTTMGGRNVKIEFYTAEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINFDQFALWYSQAGTPVLEAESRL
                     KNNIFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAMAFDYQGKRATEAVLLLT
                     EAEQTFQFESVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIELPKHEKLLAAVEKVISDDLLGNAFKALLLGVPSEAELWEGT
                     ENIDPLRYHQAHEALLDTIAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(2993..3787)
                     /locus_tag="WGR74_001702"
     CDS             complement(2993..3787)
                     /locus_tag="WGR74_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0906074.1"
                     /translation="MTTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQIVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLLYGINAVRYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHAGKLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            complement(4536..5405)
                     /locus_tag="WGR74_001703"
     CDS             complement(4536..5405)
                     /locus_tag="WGR74_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ0906075.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIADLTGLLAYRLVKPRRRIGEIN
                     LAKCFPEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AVGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLHFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5456..5992)
                     /gene="ruvC"
                     /locus_tag="WGR74_001704"
     CDS             complement(5456..5992)
                     /gene="ruvC"
                     /locus_tag="WGR74_001704"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ0906076.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADAS
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(5995..6234)
                     /locus_tag="WGR74_001705"
     CDS             complement(5995..6234)
                     /locus_tag="WGR74_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ0906077.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6265..7275)
                     /gene="dusB"
                     /locus_tag="WGR74_001706"
     CDS             complement(6265..7275)
                     /gene="dusB"
                     /locus_tag="WGR74_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ0906078.1"
                     /translation="MRIGGYIIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTKKTLHRSDFTDEGGVAAVQIAGSDPRQMADAARYNVSLGAQVIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVRAAGVPVTLKTRLGWHDDHQNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGDITSPQKAAAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNHVRAMHAFYGETAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSTAAQYDMLAGYLERLAGKTDRWACAYRQN
                     AV"
     gene            7643..9016
                     /locus_tag="WGR74_001707"
     CDS             7643..9016
                     /locus_tag="WGR74_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0906079.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGVNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVTAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKTEMREPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
BASE COUNT         2138 a         2514 c         2334 g         2190 t
ORIGIN      
        1 gtattttaat taaccttaat cggtgcactt ctagcaatat agtggattca caaaaaccag
       61 tacagcgttg cctcgcctta ccgtactatc tgtactgtct gcggcttcgt cgccttgtcc
      121 tgatttttgt taatccacta taattaatat gactttgcgg ccgttttgcc attgcgtaat
      181 aaaacgatgg ggaagtgatg ataaaacgtg tgtgtaacta tatcagacgg cattgttttt
      241 ctgtttgacg gcctcaatcc aaaattttgc cgacgatttc gcccacgtct ttcgacaatc
      301 cttcctgcgc ccgaatgcgc tgcaatgctt gtttcaccaa gtttttgcgg tgcggctcga
      361 gcttgttgca gaggttgaac gcctgcacta agcgggcggc gacctgcggg ttaaagcggt
      421 cgatttcgat gactttgtcg gcgatgaagc ggtagccgct gccgtcttct gcgtggaaat
      481 gcgggacgtt gcggctgaag ctgccgataa gcgagcgggc tttgttgggg ttttcgaggc
      541 tgaatttcgg atgctgcaag gcagtttgaa cctgttgcag ggtgtcgctg cggcggcttg
      601 agccgacgag ggcgaaatat ttgtccatca ccagcgcatc atccgaaaac ttgtcggcaa
      661 actgagccag caggcggttg cgcgtatcgc tttcgttgcc gttgacggcg gacaggatgc
      721 cccattcgtg ggtcatgttt tgcgccattt cgccgtattt ttcggcaacg gtttcgatgt
      781 gcgcggggtc ggcgcgcagg acgaaggcgc ggcagacgtt gcgcagcgtg cgccagccgg
      841 cagcttcggg gctgtactcg tagctttggt tttcctgctt cgccgcctga cggttcaatt
      901 cgtgccattt cggcaggaag cggacggcga ttgtatccaa caaggcttcg tgcgcctgat
      961 ggtagcgcaa cgggtcgatg ttttccgtgc cctcccacag ctcggcttca gacggcacgc
     1021 ccaaaagcag ggctttgaag gcgttgccta agaggtcgtc tgaaatgact ttttcgacgg
     1081 cggcaagcag tttttcgtgt ttcggcagtt caataccgtc tgaaagtgcg gcaaggttgg
     1141 cggcaacggc acggcggtag agcgtttgtg cggcttccca gcgcgtgaag gcgtcgctgt
     1201 catgggcgag cagaagcagc aggtcgtcgt cgctgtacgg atagttcaaa tgcaccggcg
     1261 cgctgaaccc gcgcagcagc gagggaacga cggcttcggt tacgctttcg aactggaagg
     1321 tctgttcggc ttcggtcagc agtaacacgg cttcggttgc gcgtttgccc tgataatcga
     1381 atgccatcgc ttcgccgttg cggttcaaca gtccgacttt gacgggaatc atcatcggct
     1441 gcttgtccgc catatcgggc gtgggcggca cggtttgttt aatggtcaac tcgaaaatat
     1501 tgtttttcag acggctttcc gcttccaaaa cgggcgtgcc cgcctggctg taccacaagg
     1561 cgaactggtc gaaattgatg ccgttcgcgt ccgccatcgc cgcgcggaaa tcgtcgcagg
     1621 tcacggcctg cccgtcgtgt cgttggaaat agagcttcat gcctttttgg aagccctctt
     1681 cgccgagcaa ggtgtgatac atccgcacca cttccgcgcc tttttcataa acggtcatgg
     1741 tgtagaaatt gttcatctcc tcatagctgg cggggcgcac cggatgggcg gtcgggcctg
     1801 cgtcttcggg gaactggtgc tggcgcagca ggcggatgtt ctcgatgcgg cgcacggcgc
     1861 ggctggcgcg gtcgccggaa aattcttggt cgcggaacac ggtcaaccct tctttcagcg
     1921 aaagctggaa ccaatcgcgg caggtaacgc ggttgcctgt ccagttgtgg aaatactcgt
     1981 gtccgaccac ggattcgatg ccttcaaaat cggtatcggt ggcggtacgg ctgtcggcaa
     2041 ggacgaactt ggtattaaaa atgttcagcc ccttgttttc catcgcaccc atattgaaat
     2101 cgcccacggc gacgaccatg aaaatatcca agtcgtattc caaaccgaag cgcgtttcgt
     2161 cccacttcat cgcgtttttc aaggattcca cggcaaagcc gaccttaggc ttgtccgcct
     2221 cggcagtgta aaactcgatt ttgacgtttc tgccgcccat cgtggtgaaa cagtcttccg
     2281 tgaccgccaa atcgcccgcg accaaagcaa acagatagct cggtttggca aacgggtctt
     2341 cccatttcac ccaatggcgg ccgtctgaac actcgccgcc gtcgattttg ttgccgttgg
     2401 agagcaaaac gggatagcgt tttttgtccg caacgatggt ggtcgtgaat ttggacatca
     2461 catccggacg gtcgatgtag aacgtgattt tgcggaagcc ctccggctcg cattgggtaa
     2521 acagattgcc accggaagca tacagcccca tcagcgattt gttttccgcc ggcaggatct
     2581 cggtttccac ttcgacggtg aagcgttcgg acggcacgtc tgcaatcgtc agagtctcgc
     2641 cttccaacac ataatccgcc gccacgccgt tgattttgac ggacaagagt ttcgccgaac
     2701 cgtccaacac cagcggctcc cctaccctct gcggctcaac cgtcaaacgg gatttcacga
     2761 cggtttgcgg ttcggcaata tcgaaatgca ggtcggtttc gagaatacgg taggcaggcg
     2821 tttggtaatc tttcagataa cggacggttt tgctcatttt tttctttcaa tattgtttgg
     2881 tttgactgga aaaggcttca gacggcatag gcaaaccctg caccgatatg ccgtatgaaa
     2941 ccgaaacatc agtgcggacg caccgtcaaa caaccggttt taatttcaat ctttatttcc
     3001 acgcttggac aaattctgcc caatgcggtt tgtcttctgt ttgggcggaa agatagtttc
     3061 tgatccgctt gatttccatt tcgtattcgt ccgcgtcgaa tctgccgctg accagacaaa
     3121 aacgcagata catcaaatac gtattcacgg catcggtttc acaataattg cggatttcct
     3181 tcaatttacc ggcatggaat gcctcccaga ccttgctgcc gtccataccc aatttacccg
     3241 gaaaaccgca cagtttcgcc atatcgtcca gcggcacatt cgccctcggc tggtaaagcg
     3301 cgagcaaatc catcaaatcg caatgacgtt ggtggtagcg gctgatgtag ttgttccact
     3361 tgaaatcgcg gctgtcgccg aaatcgccgt cgcccatatc ccaatagcgc acggcgttga
     3421 tgccgtacag caatgaacgg taatgcagga caggcaggtc gaaaccgccg ccgttccaac
     3481 tgaccagttg cggcgtatgt ttttcaatca actcgaaaaa tttggcaatc acgacttctt
     3541 cgccatcgtc catctcgccg attgtaccga catggacttt atcctgcccc caacgcatgc
     3601 agcacgaaat cgccacaatc tgatgaagat gatgctgcat aaaatcccca cccgtctgag
     3661 cacggcgttt ttgctgggca aacagcacca cttcatcgtc gggcagcgag gacggcagct
     3721 cgtacaatgt tcggataccc tgcacatcgg gtacggtttc aatatcgaaa gccaaaatcg
     3781 tggtcatgac agcaccttgt atttaaaaca gacttgcgcc tattgtgtca ttaaaaggcc
     3841 gataaaaaaa gaggcaaccc cccacaggat tgccccaata cctcaaatca gagatttacg
     3901 cttcacaaac aatacaggct ttcgcctgcg gctttacccg cgtagctcaa ctctacgccg
     3961 gcaaactttt gtttcaccgt ttccgatgaa accccgacca atcgcaagac tgaccggaaa
     4021 atcctttcaa acagcatttc ctgcctgtcg tgtaattcca tgtagcgaaa tgtacgccat
     4081 tttctacgct ttgccaagca ttttttacaa tataaatgtc aaaacattaa ttttataaaa
     4141 ttgctgaaaa tattaaatat atggattttt attttttata tttcaataaa tataaattta
     4201 attttgattt atatttaaat ttaagcataa aatgtcaaat attaaaggaa acatgaaagg
     4261 catatattaa atatttattt ataacgctat gtttttaaag aaaattaatt ttaatatatt
     4321 aactagattg tctgcatata ttcatgggtt tgcggtattt cttccaaaac ctgcttcgaa
     4381 tttcccgacc aagtcttaaa aatattgttt ttgagatact taaatagcag cgattatcaa
     4441 atgaaatctg ttcatataat ctgccatttt gcatttaaaa aaacaatcag gagtttcgac
     4501 tcgaaatgcc tgatatgttt tgtaatttta cgtagtcagt aaaaatcggg gctgccttcc
     4561 ggacgggttt taaaacgctt gtgcagccaa aaatactgct cgggatgttc gcgcacgcgt
     4621 tcctcgataa aacggttcat gcgctgcgcg tcggcttgcg catcttcact cggaaaggat
     4681 ttccaagccg ggtagaaatg cagtgtaact gtattgtctg cctcgcggac gggaatggcg
     4741 ggtatcactt ttgcatttgc aagcgcggca atgcggctca atccggtaat cgttgccgtc
     4801 tgaataccga agaaatccac aaaaaccgaa tcgttgcgtc cgaaatcctg atcgggcaga
     4861 tacagaaacg gcgcgctgct tttgcggaac tgtttgacga gggcgcgcag cccttcggtg
     4921 cgcccgataa ggaagacgtt gtgatagcgg ttgcgacctt tcaaaatctg ttcgtccaat
     4981 atcttgtttt tttgatggga atacatactg atcagcggga catcctgatt aagcgcgtac
     5041 accgccatct cgaacgcggt gaagtgcgga tacaggatga tgactttttc ccccaccgcc
     5101 agcgcgtcgt ccaaataatg cttattgcgg taacgcacca gcgatttcaa acgcccggca
     5161 ggcgcgtacc aatataaacc gtattccaac atcagtttcg ccatgtgttt gaaatgctgt
     5221 ttcaacacgg ttttacgctt ttcctcactc cattcgggaa aacattttgc caaattgatt
     5281 tcgccgatac ggcggcgcgg tttgaccaga cggtaggcaa gcaaacccgt caggtcggca
     5341 atcttatgca gcagcgcaaa cggcagaaac tgcaaaacat acagtacaaa aaatataaat
     5401 ttcatctcga tacacatttt cttttcagac ggcaaaatac aaatgccgtc tgaaactatt
     5461 gaaacctgcc gcgcttgacc tgcatccctg aaggattgag tttggcggca agcccgtggt
     5521 tgcgtaaggc gtgggtcagc gcgacggcaa gaccgtccgc cgcatccgcc tgcggcgttc
     5581 ccgaaagccc cagcatctgc accaccatat gctgcacctg ttctttcgcc gccttgcctt
     5641 tgccgactac cgcctgtttg acctgcaagg cggtgtattc cgaaacgggc agcttatggc
     5701 tgaccaatgc cgccaatgcc gcgcccctag cctgacccag catcagcgtc gatgcaggat
     5761 tgacgttgac gaacacctgt tccactgccg cctgttgagg cttgtaaacg gcaacgactt
     5821 cgccgatatg ccgcacaatc acggcaatcc tgtctgccag agacgcatcg gcaggcgttt
     5881 tgatgcagcc ggaggcaaca taaaaatgat cccgccccct gacatcgatg atgccgaaac
     5941 ccgttacgcg gctgcccggg tcaatgccca agatacggac ggttgccgac atattcacaa
     6001 caaaccgtgt tgaatcagtt ttttacgcaa ggtattgcgg ttcagcccca gcatcacgga
     6061 tgctttggac tggttgccgc cgcattgttc catcacgcac accagcagcg gtttttccac
     6121 ctgatgcaat accatatcgt acacgccgca aggttcggta ccgttcaggt ctttgaaata
     6181 ttgttccaaa ttttgtctga tacattggga aatatcggga agggtatggg gcataatcgc
     6241 gctttcaaaa ggataatcgg gtgttcagac ggcattttgg cggtaggcgc acgcccaacg
     6301 gtcggttttt ccggcaagtc tttcaagata acctgcaagc atgtcgtatt gcgccgccgt
     6361 actgtccaag cggttgattt cacgacgtgt ctgttcgccg tcgggcattt cgtcgatgta
     6421 ccagcctatg tgtttgcgtg caatgcgcac accggcggtc tcaccataaa acgcgtgcat
     6481 ggcgcggacg tggttcaaaa tggcggcact gcattctgcc aaactcaagg caggcggtaa
     6541 aacaccgtgt tcggcgtaat gtttcaaatc gcggaagaac cacggtctgc cttgcgcgcc
     6601 gcgccctatc atgatgccgt cggcggcggt ttgtttgagg acggcggcgg ctttttgcgg
     6661 cgaagtgatg tcgccgttga cccagaccgg gatgttcaga cggcatttgg tttcggcgat
     6721 gagttcgtaa cgcgcctcgc ctttgtacat ttgcgtgcgc gtgcgtccgt ggacggcaag
     6781 ggcggcgatg ccgcaatctt cggcgatttt ggcgatgacg ggcaggtttt gatggtcgtc
     6841 gtgccaaccc aaacgggttt tgagggtaac gggtacacct gccgcacgga cgacggcttc
     6901 caaaatggcg gcaaccagcg gctcgttctg catcagcgcg ctaccggctt ggacattgca
     6961 gactttttta gcgggacagc ccatgttgat gtcgataacc tgcgccccaa ggctgacgtt
     7021 gtaacgcgcg gcatccgcca tctgccgcgg atcgctgccg gcaatctgca cggcggcgac
     7081 accgccttca tcggtaaaat cactgcggtg cagggtcttt ttggtatttc tgagcgtcgg
     7141 gtcgctggtc agcatttcgc acaccgccca acccgcgccg aaatcgcggc aaaggcggcg
     7201 gaacggtttg tccgtaatgc ccgccatcgg ggcgagtgca atgggattgt cgataatgta
     7261 gccgccgatg cgcataatgg gtccgcgttt caaaaaagtg caacattgta cattttttaa
     7321 gcaggatttc caatctccgg acgcacccac gattggggcg ggcaccgttt tatggcataa
     7381 tccgcacaca gattccctgc cccgccactc acaggagggc agtttatagt ggattaacaa
     7441 aaaccagtac ggcgttgcct cgccttagct caaagagaac gattctctaa ggtgctcaag
     7501 caccaagtga atcaattccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga
     7561 tttttgttaa tccactatac tttccccgtc ctatcggttt cccgtttcag acgacataag
     7621 gtctgaaaga aagactacaa ttatgagtaa tccattttcc tctttaggtt tgggtacgga
     7681 actcgtttcc gcactgaccg cgcaaggtta cgaaaacccg acgcccatcc aagccgccgc
     7741 cattcccaaa gcactcgccg gtcatgattt actagccgcc gcgcaaaccg gcacgggcaa
     7801 aaccgccgcc tttatgctgc ccagtctgga acgcctcaaa cgttacgcca ccgccagcac
     7861 ctcgcccgcg atgcaccccg tgcgtatgct cgtgttgacc ccgacgcgcg aacttgccga
     7921 ccaaatcgac caaaacgtgc agggctacat taaaaacctg ccgctgcggc ataccgtcct
     7981 cttcggcggt gtcaacatgg acaaacaaac cgccgacctg cgtgccggct gcgaaatcgt
     8041 cgtcgccacc gtcggacggc tgctcgacca tgtgaaacag aaaaacatca gtttaaacaa
     8101 agtcgaaatc gtcgttttgg acgaagccga ccgtatgctg gatatgggtt ttatcgacga
     8161 catccgcaaa atcatgcaga tgctgccccg ccaacgccaa accctgctct tttccgccac
     8221 cttctccgcc ccgatacgca aactggcgca agacttcatg aacgcgcccg aaaccgtcga
     8281 agtcaccgcg caaaacacca ccaacgccaa cgtcgaacaa cacatcatcg ccgtcgatac
     8341 cattcagaag cgcaacctgc tcgaacggct gattgtcgat ttgcatatga accaggtcat
     8401 cgtgttctgc aaaaccaaac aaagcgtcga ccgcgtaacg cgcgaactgg tgcgccgcaa
     8461 cctgtccgca caggcgatac acggcgaccg ttcccaacaa agcaggctcg aaacactcaa
     8521 cgccttcaaa gacggcagcc tgcgcgtcct cgtcgccacc gacatcgccg cgcgcgggct
     8581 ggacattgcc gaactgccct tcgtcatcaa ttacgaaatg cccgcccagc ccgaagacta
     8641 cgtccaccgc atcgggcgca cggggcgcgc gggcgcggac ggcgtggcga tttccctgat
     8701 ggacgaatcc gaacagaaaa tgtttgaatc cattaaagag ctgaccggca acaagctgct
     8761 catcgagcgc atcgagggct tcgagccgca atggtgggaa cagggcggcg caaaaccgga
     8821 aaaaaccgaa atgcgcgaac cgagacaacg caaccgctac gaatccgcca gggctcagcg
     8881 cgaaaaaaat acccgaccgg aaaccacggc aaacgatgcg ggcgcggctt gcggaaaaat
     8941 tgccggacgc agccgccgaa gccgccggga acaccggacg tgcgccctgc tccaaccgcg
     9001 ttacggcgta aaataaccct gaaaatcaaa tgccgtctga acatttcccg tttcagacgg
     9061 catttttcaa actggactga cgcattgggc gcaaccgccc gcacaggata aatttcttcc
     9121 gcaaacagtt tcagacggca tttgccgcct gtacaatata gtggattaac aaaaat
//
LOCUS       DAWFLT010000074         9032 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWFLT010000074 DAWFLT010000000
VERSION     DAWFLT010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9032
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.074"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            69..2183
                     /locus_tag="WGR74_001708"
     CDS             69..2183
                     /locus_tag="WGR74_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ0906080.1"
                     /translation="MFLFSDGLQSINNNNRRKRIVKNAYIPSRGIRKIPYLSTLLPEF
                     HICKDGKGAEAVIGWGLRPTTHKARAFATEHQLPFIALEDGFLRSLGLGVSGYPPYSI
                     VYDDIGIYYDTTRPSRLEQLILAADTMPSETLAQAQQTMDFILQHHLSKYNHAPELSD
                     DHPLRSPSKPETVLIIDQTFGDMAIQYGGADASTFELMFQTALNENPQADLWVKTHPD
                     VLCGKKQGYLTQLAQQHRVHLLAEDINPISLLQAVDKVYCVTSQMGFEALLCGKPLVT
                     FGLPWYAGWGVSDDRHPEINRLVQTQRRATRNLLQLFAAAYLQYSRYLNPNTGEAGSL
                     FDVIDYLATVKRKNDKLRGELYCVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVK
                     LSDDARILAWGNGKEAIVRFAEQHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMSI
                     YFNAETPSRLEYILQNQNFDDQDFQTALKLQKMLTENHISKYNVGSSDFTAPSTDKTV
                     ILVPGQVEDDASIRYGSPQIYRNLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISP
                     EDAARYADQTAEQTDILTCLQYADEVHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLT
                     QDLLPIPRRSRRLELWQLIAGTLIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNN
                     GLHRGYFAKKLGKIKQLYRSFK"
     gene            2320..3579
                     /locus_tag="WGR74_001709"
     CDS             2320..3579
                     /locus_tag="WGR74_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide modification protein
                     LipB"
                     /protein_id="HEZ0906081.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSNSSTPVI"
     gene            3947..5353
                     /locus_tag="WGR74_001710"
     CDS             3947..5353
                     /locus_tag="WGR74_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ0906082.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYTEAAFVLG
                     NHLLKNGHPEQALPWLEAAAAQRHPKALFTLLQQREHNGTPTGQLLNEYAWLSEQGHL
                     EAQLILMRYHTQRNDPQSLYWAELAAAQYAAPAYYHLARHHQRQGDVATAIEQYEKAA
                     ALGITAACWQLGQIYFYGTGVSPNHAQAEHYLEQAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAANKEQAEAQAKLAQYALTGELSERDPFQAARYAKAAAEKDHPEALKIMGD
                     LYRYGLGIKADNHIAHDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            5571..6785
                     /gene="gltS"
                     /locus_tag="WGR74_001711"
     CDS             5571..6785
                     /gene="gltS"
                     /locus_tag="WGR74_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ0906083.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     IAAIVLFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            6884..7900
                     /locus_tag="WGR74_001712"
     CDS             6884..7900
                     /locus_tag="WGR74_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0906084.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSVALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDADVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            7911..8108
                     /locus_tag="WGR74_001713"
     CDS             7911..8108
                     /locus_tag="WGR74_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906085.1"
                     /translation="MNTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAV
                     RLGENGFTEEQMENIANLFGR"
     gene            8126..8275
                     /locus_tag="WGR74_001714"
     CDS             8126..8275
                     /locus_tag="WGR74_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906086.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVAYADVSTHRTIQKTS
                     PYVPS"
     gene            8631..8924
                     /locus_tag="WGR74_001715"
     CDS             8631..8924
                     /locus_tag="WGR74_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906087.1"
                     /translation="MKTNDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSVHN
                     RKADYAEKGKEQIALFPRGLNGITKLPAAVLLPERPYHFDMKEVLHIFSWIPR"
BASE COUNT         2312 a         2606 c         2103 g         2011 t
ORIGIN      
        1 aaagacttgg caggcaaagt attggccgaa gcagtcacgt tttaagacta ttcaggccgt
       61 ctgaaagaat gttcctcttt tcagacggcc tacaatccat taataacaat aatcgacgaa
      121 aacgcattgt gaaaaacgcc tacatcccct ctcgcggcat ccgcaaaatc ccctatctct
      181 ccactctatt gcctgagttt catatctgca aggatgggaa aggagcagag gctgttatcg
      241 gctggggttt gcgcccgacg acacacaaag cgcgtgcttt tgccaccgaa caccagcttc
      301 cctttattgc tttggaagac ggctttttac gctcgctcgg actgggcgtc tccggttatc
      361 ccccctactc catcgtctat gacgacatcg gcatctacta cgacactacc cgcccttctc
      421 gtttggaaca actgattctt gccgccgata ccatgccgtc tgaaaccttg gctcaggcgc
      481 aacagacgat ggatttcatc ctgcaacacc acctgtccaa atacaaccac gcgcccgaac
      541 tttcagacga tcatccttta cgctccccat ccaagcctga aactgtcctc attatcgacc
      601 aaaccttcgg cgatatggcc atccaatatg gcggcgcaga cgcctctacg tttgaactga
      661 tgtttcagac ggccttaaat gaaaacccgc aagccgatct ttgggtcaaa acccaccccg
      721 atgttttgtg cggcaaaaaa caaggctatc tgacccagct ggcgcagcaa caccgcgtcc
      781 atcttttggc tgaagacatt aatccgattt cgctgctgca agcggtggac aaagtgtatt
      841 gcgtgacctc gcaaatgggt tttgaagcct tgttgtgcgg caagcctttg gtaacgtttg
      901 gtttgccgtg gtatgccggc tggggtgtaa gcgacgaccg ccatcctgaa atcaaccgcc
      961 ttgttcaaac ccaacgccgc gccacccgca acttgctgca gctcttcgcc gcagcctatc
     1021 tgcaatacag ccgctacctc aaccccaata ccggcgaagc aggcagcctc tttgatgtca
     1081 tcgactatct ggcgacggtc aaacgtaaaa acgacaaatt gcgtggcgag ttatattgcg
     1141 tcggtatgtc tttgtggaaa cgcgcggttg ccaaaccgtt ctttaacgta ccctcttgcc
     1201 gtctgaaatt tatctcttcc acccaaaaac tggcaagggt caaactgtcc gacgatgcac
     1261 gcatcctggc ttggggcaac ggcaaagagg ccatcgtccg ctttgccgaa caacaccaca
     1321 tccccctgct gcgcatggaa gacggcttta tccgctcggt cggactcggc tccaacttag
     1381 tgccgccgct gtcgctcgtt accgacgata tgagcattta tttcaatgcc gaaaccccgt
     1441 cccgtcttga atacatccta caaaaccaaa acttcgacga tcaagacttt cagacggcct
     1501 tgaagctgca aaaaatgctg accgaaaacc acatcagtaa atacaacgtc ggcagctcag
     1561 acttcaccgc cccgtcaacc gacaaaaccg tgatcctcgt tcccggccag gttgaagatg
     1621 atgcgtctat ccgctacggt tcgccccaaa tctaccgcaa tctggatttg ctccgtaccg
     1681 tacgcgaacg aaaccccaat gcctatatca tctacaaacc gcatcccgat gtagtcagcg
     1741 gtaaccgcat cggccatatt tcccctgaag atgctgcacg atatgccgac caaaccgccg
     1801 agcaaaccga catcctgacc tgtctccaat acgcagacga agtacatacc atgacttcgc
     1861 tgaccggttt tgaagccttg ttgcgcggca aaaaagtcag ctgctacggc ctgccttttt
     1921 acgcaggctg ggggcttacc caagatctgc tccccatccc gcgccgtagc cgcagacttg
     1981 agctttggca gctgattgcc ggcacgctca tccactatcc cgactacatc caccccgaaa
     2041 cccatcaggc cataaatgca gaaaccgcag cccaaatcct gatacgacaa aaaaatatgc
     2101 aaaaaaacaa caacggatta catcgcgggt actttgccaa aaaattaggt aaaatcaaac
     2161 aactatatcg atctttcaaa taaataccat caaagttaac gatgcgtcat aaacttgcct
     2221 ctattgcggc atcattgcct ttgcatcgtt aattctcttg gcgtatgctt gaaagttcaa
     2281 cctaaaacta ttacataaaa aacaaaacca cattgcaaca tgaaacagac cgtcctcaaa
     2341 aataacctgc aaaacctgct tgaaagcgca gaaaatatcc tgctgcttca aggccctgtc
     2401 ggcgattttt ttctgcgcct tgccgactgg ctgactgcaa acggcaaaac cgtacataaa
     2461 ttcaacttta atgcaggcga cgactatttt tatccgccca ctcaagcgca taccgttgtt
     2521 tttaacgaca actacgatgc ctttcctgag tttttgcaag aatacatcac tcaacatcac
     2581 atccaggccg ttgtctgctt tggcgacaca cgcccttatc acgtcattgc aaaacgcatt
     2641 gcaaacgaaa accaagccag tttctgggcg tttgaagaag gctatttccg cccctactac
     2701 atcaccttag aaaaagacgg cgtcaacgca ttttccccgt tgccgcgccg tgccgacttt
     2761 tttcttgaac aattccctaa gcttgcccag caagaatata aagcgccaac gccggtacac
     2821 ggcggtttta cgcccatggc aaaaaacgct atccgttact atatcgagtt gttccgcaat
     2881 ccacgcaaat accccgacta catccaccac cgcgcaccca atgccggcca ttacctcaaa
     2941 ccgtggtcgc tctccatcct caagcgtttg aactactata ttgaagacat ccaaatcgcc
     3001 aaacgtgtgg aagcaggcaa atacggcaag ttttttattg ttcccttaca ggtattcaac
     3061 gacagccaag tccgtatcca ttgcgacttt cccagtgtcc gcagcttcct gctccatgtt
     3121 ttgagttcat ttgccgaaca cgcgcctgcc gataccaaca tcatcatcaa gcatcatccg
     3181 atggaccgcg gttttatcga ctactggcgc gacattaaac gctttatcaa agaacacccc
     3241 gaactcaaag gccgcgtgat ttatgttcat gatgtccccc tgcccgtttt cctgcgccac
     3301 ggtctcggca tggtcaccat caacagcacc agcggcctgt ccggcctgat tcacaatatg
     3361 ccagttaagg ttctcggccg tgcctattat gatattcccg gcattactga ccaaaatacc
     3421 ttggcagaat tttggaatca tccgacaccg cctgacaaag agctgtttca tgcctaccgt
     3481 atgtaccacc tcaacgtgac ccaaattaac ggcaacttct acagtcaggt gtttttcccc
     3541 aacaaaaaaa cctccaactc ttccacacca gtaatctgac ttagcgaagg aaagttcagg
     3601 ccgtctgaaa acatttcaga cggcctgaaa caatcaatac cttagctact gccatgtaaa
     3661 taaaacacaa aaacctgcat ttatcattaa caataaatta caaaaacagt ataatgaccg
     3721 agctgtcatg agcgcatacc gactcaacct gagccctttg taacacacaa aatatggatg
     3781 tatccctagg caaaacaata taacaagcca aacatcctaa agataagccg gcaaggcaac
     3841 acactctaca aaaccgtgcc aagcaatatt tttacaaagc cctcaaccgg tatcgccgcc
     3901 catatgccgc agcatccgtc ttccacttta tatccgcccg caaaccatga ccgccgctcc
     3961 tgatatcctc taccggcaag ccgccgccct tttggaacaa tccaataccg cccaagccct
     4021 gcccctgctg caacaggccg cagagcaggg atatacggaa gctgcttttg tattgggcaa
     4081 ccatctgctg aaaaacggtc atccggagca ggcacttcca tggttggaag ccgccgcggc
     4141 ccaacgccat cccaaggcac tcttcaccct gctgcaacaa cgcgaacaca acggcacccc
     4201 gaccggacag cttcttaacg aatacgcctg gctgagtgag caaggtcacc tcgaagccca
     4261 attaattctc atgcgttacc acacgcagcg caacgatcca caatcgctct actgggcgga
     4321 gcttgccgct gcccaatatg ccgcacctgc gtattaccat ttggcacgcc atcatcaacg
     4381 ccaaggcgac gttgcgacag ccatcgaaca atacgaaaaa gcggcagcac tcggcataac
     4441 tgctgcctgc tggcaactcg gtcaaatcta cttctacggt acaggtgtca gccccaacca
     4501 cgcacaagcc gaacactatc tcgaacaagc ggcgcaagcc ggccacatcg ccgcacaaac
     4561 cctgctggcc gaccttcttg ccgcccaacg caaacccgaa gccttagaat ggtatcgtcg
     4621 tgccgccaat aaggagcaag cggaagcaca agccaagctg gcccaatacg ccctgaccgg
     4681 cgaactttcc gaacgcgatc cgttccaagc ggcgcgctat gccaaagccg ctgccgagaa
     4741 agaccatcct gaagccctga aaatcatggg cgacctctac cgctacggcc tcggcatcaa
     4801 agctgacaac catatcgcgc acgattacta ccaccgtgct gccgcgctgg gttctgccgc
     4861 cgcagcacaa aaactcatca gcgacgccgc gctgtaccat ccgcaacaat acgaacaaat
     4921 caaaactgcc gccctgcaac aacaacaaac cgaaaccatc taccgtttgg cggaagcaca
     4981 agcctgcgcc atcggccgtc ccgccgacta caatgccgcg cgaaaaaatt acatggaagc
     5041 tgccgggttc caccataaaa acgcagcggc agccttaggc cgcatctacc attacggcct
     5101 cggtacggcg caagatcctc gggcggctgc acactggtac gccattgctg ccgaacaaaa
     5161 ccacccttcc gcccaatacc acctcgcctg tttttactat cacgggcaag gtgtcggctg
     5221 tcatgttccg accgcctgct actggctgca ggccgccatc ggcaacggcc acacttcggc
     5281 cgaatcatta atatccctat tagaacaatg gcgacgcgaa gcacaccatg ccatcggaca
     5341 aaaggccgtc tgaaaagatt tacactcgca ttttttgaca atctttaact attcccctaa
     5401 tatttgccag ttatttttca cggacacgcc attgttttca tttctttctg aaaacacctt
     5461 gtccgcgcat caataccatg acactcggcg gataacgcca agcgttgaaa cacactacat
     5521 ccggaacaaa aacggatgct cggaaaaata tttctaggag gtgaaacaac atggaatggg
     5581 aattcaacag ttattacaca ctgattgccg ccacgctcgt gttgctggtt ggtaaatttc
     5641 tggttcaaaa aatcaaattc ttacgagact tcaatattcc cgagccggta gccggcggtt
     5701 tgattgccgc tatcgtcctg ttcgccctgc acgaggcgta cggcgtgagc ttcaaatttg
     5761 agaaaccgct gcaaaatgcg tttatgctga ttttcttcac gtccatcggc ttgagcgcgg
     5821 atttttcccg tttgaaggcg ggcggtttgc cgctggtggt ttttaccgcg attgtgggcg
     5881 gatttatctt ggtgcaaaac tttgtcgggg tcggactggc tacggctttg ggtttggatc
     5941 cgctcatcgg tctgattacc ggttcggtgt cgctgacggg cggacacggt acgtcaggtg
     6001 cgtggggacc taattttgaa acgcaatacg gcttggtcgg cgcaaccggt ttgggtattg
     6061 catcggctac tttcgggctg gtgttcggcg gcctgatcgg cgggccggtt gcgcgccgcc
     6121 tgatcaacaa aatgggccgc aaaccggttg aaaacaaaaa acaggatcag gacgacaacg
     6181 cggacgacgt gttcgagcag gcaaaacgca cccgcctgat tacggcggaa tctgccgttg
     6241 aaacgcttgc catgtttgcc gcgtgtttgg cgtttgccga gattatggac ggcttcgaca
     6301 aagaatatct gttcgacctg cccaaattcg tgtggtgtct gtttggcggc gtggtcatcc
     6361 gcaacatcct cactgccgca ttcaaggtca atatgttcga ccgcgccatc gatgtgttcg
     6421 gcaatgcttc gctttcgctt ttcttggcaa tggcgttgct gaatttgaaa ctgtgggagc
     6481 tgaccggttt ggcggggcct gtaaccgtga ttcttgccgt acaaaccgtg gtgatggttt
     6541 tgtacgcgac ttttgttacc tatgtcttta tggggcgcga ctatgatgcg gcagtattgg
     6601 ctgccggcca ttgcggtttc ggcttgggtg caacgccgac ggcggtggca aatatgcagt
     6661 ccgtcacgca tactttcggc gcgtcgcata aggcgttttt gattgtgcct atggtcggcg
     6721 cgttcttcgt cgatttgatt aatgccgcga ttctcaccgg ttttgtgaat ttctttaaag
     6781 gctgattttc cgcctttccg acaaagcacc tgcaaggttt accgcctgca ggtgcttttg
     6841 ctatgatagc cgctatcggt ctgcaccgtt tggaaggaac atcatgtatc ggaaactcat
     6901 tgcgctgccg tttgccctgc tgcttgccgc ttgcggcagg gaagaaccgc ccaaggcatt
     6961 ggaatgcgcc aaccccgccg tgttgcaagg catacgcggc aatattcagg aaacgctcac
     7021 gcaggaagcg cgttctttcg cgcgcgaaga cggcaggcag tttgtcgatg ccgacaaaat
     7081 tatcgccgcc gcctacggtt tggcgttttc tttggaacac gcttcggaaa cgcaggaagg
     7141 cgggcgcacg ttctgtatcg ccgatttgaa cattaccgtg ccgtctgaaa cgcttgccga
     7201 tgccaaggca aacagccccc tgctgtacgg ggaaaccgct ttgtcggata ttgtgcggca
     7261 gaagacgggc ggcaatgtcg agtttaaaga cggcgtattg acggcagccg tccgcttcct
     7321 gcccgtcaaa gacggtcaga cggcatttgt cgacaacacg gtcggtatgg cggcgcaaac
     7381 gctgtctgtc gcgttgctgc cttacggcgt gaagagcatc gtgatgatag acggcaaggc
     7441 ggtaaaaaaa gaagacgcgg tcaggatttt gagcggaaaa gcccgtgaag aagaaccgtc
     7501 caaacccacg cccgaagaca ttttggaaca taatgccgcc ggaggggatg cagacgtacc
     7561 ccaagccgca gaaggcgcgc ccgaaccgga aatcctgcat cctgacgacg gcgagcgtgc
     7621 cgataccgtt accgtatcac ggggcgaagt ggaagaggcg cgcgtacaaa accagcgtgc
     7681 ggaatccgaa attaccaaac tttggggagg actcgatacc gacgtgcaaa aagagttggt
     7741 cggcgaacaa cgcaagtggg cgcaggaaaa aatcagcaac tgccgacaag ccgccgcgca
     7801 ggcagaccgg caggaatacg ccgaatacct caagctgcaa tgcgacacgc ggatgacgcg
     7861 cgaacggata cagtatcttc gcggctattc catcgattag gggcaaaccg atgaataccg
     7921 tcccaaaaag caggattccc gtcaaaccgc tgcccgaaaa aaccacagac gaagccaaag
     7981 tcgaaaaatg gcggcagctc ggtgcggaac acggtttgtc gggcgaatgg gcagttgccg
     8041 tcagattggg cgaaaacggt tttaccgaag aacagatgga aaatatcgcc aacctgttcg
     8101 gcagataaag agaaaattga cggaaatgcc gtctgaaacc ctgttatcgg tttcagacgg
     8161 cattttgacc aatacggtac gcaggcgcaa aacagccggc ttttcctgtg ttgcctatgc
     8221 tgatgtttca acacacagga cgatacaaaa aacgtcgccc tatgtgccgt cctgattcgg
     8281 aagggttacg ctccttccaa atatagtgga ttaacaaaaa ccggtacggc gttgtctcgc
     8341 cttagctcaa agagaacgat tctctaaggt gctgaagcac caagtgaatc ggttccgtac
     8401 tatctgtact gtctgcggct tcatcgcctt gtcctgattt ttgttaatcc actatattgt
     8461 ccattcggca gggaaagcct gaagaagaat tttcccgcaa gatggccggc tgcctaaccg
     8521 acttggaaaa ctgtttggaa aaatacctcg aacagttcgg gccggtatcg gaaagtatcg
     8581 aagcctgtac cgtcaaactg caggagtaac cgtcgttctt caacaggctg atgaaaacca
     8641 acgacaagct caataggcag attgacgtgt tgcagaaaca gtcggcagcc atccacaacg
     8701 aagcctatat cgaaatgaac acgctgcttt accgccaccg cgaagtggtt tccgtccaca
     8761 accgcaaagc agattatgcg gaaaagggca aagagcagat tgcgctgttc ccgcgcggat
     8821 tgaacggcat caccaagctg cccgccgccg tcctgttgcc cgagcgtccc taccattttg
     8881 atatgaagga ggttctgcat atattctcct ggataccgag gtaggcgtat cggctgccgg
     8941 ataaaaatgc accccgtcat tcctgtctgt acgggggtag cggcagtgtt ttcctgcttc
     9001 gttttatagt ggattaacaa aaaccagtac gg
//
LOCUS       DAWFLT010000075         8824 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWFLT010000075 DAWFLT010000000
VERSION     DAWFLT010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8824
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.075"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(289..1185)
                     /gene="argB"
                     /locus_tag="WGR74_001716"
     CDS             complement(289..1185)
                     /gene="argB"
                     /locus_tag="WGR74_001716"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ0906088.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRVPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1355..2485
                     /locus_tag="WGR74_001717"
     CDS             1355..2485
                     /locus_tag="WGR74_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ0906089.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVRERMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNIIGDLR"
     gene            2556..3407
                     /locus_tag="WGR74_001718"
     CDS             2556..3407
                     /locus_tag="WGR74_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ0906090.1"
                     /translation="MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3490..4152)
                     /locus_tag="WGR74_001719"
     CDS             complement(3490..4152)
                     /locus_tag="WGR74_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ0906091.1"
                     /translation="MDSRKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEENGAIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            4186..4293
                     /locus_tag="WGR74_001720"
                     /pseudo
     CDS             4186..4293
                     /locus_tag="WGR74_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4352..6145)
                     /locus_tag="WGR74_001721"
     CDS             complement(4352..6145)
                     /locus_tag="WGR74_001721"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ0906092.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAKEYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWSANRLSLGKLSGRNAFKTKLADLGIELESEEALNVAFARFKELADKK
                     REIFDEDLHALVSDEMGNMNTESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            6391..7500
                     /gene="proB"
                     /locus_tag="WGR74_001722"
     CDS             6391..7500
                     /gene="proB"
                     /locus_tag="WGR74_001722"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ0906093.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDALAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIARIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            7513..8775
                     /locus_tag="WGR74_001723"
     CDS             7513..8775
                     /locus_tag="WGR74_001723"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ0906094.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRQDLEAAAGNIPESMTDRLLLDGKRICAMADGIRAVTALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGRESSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIEAHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         2011 a         2483 c         2297 g         2033 t
ORIGIN      
        1 ccgataagtt tccgtgccga caggtctaga ttcccgcctg cgcgggaatg acgaaatttc
       61 aaagttatgg cgttatcgga aaaacagaaa atcaaagccg gagaatttat cccaaacaac
      121 cggatttcaa aaaaaccaga ttcccgcctt cgcgggaatg acggatttta ggtttctgtt
      181 ttggtttctg ttttcgcggg aataacaact tagaaattgc ccgaaacccc aaaaacagaa
      241 accggacaaa cggggtttcc gccaaagccg gcattttccg actttgcttc aggcatcttc
      301 cccaccgccc aaaatcatcg aaccgatacc ggcatcggta aagatttcca gcaaaagcgc
      361 gttgggcacc ctgccgtcga tgatgtgcgt ggctttcacg ccgttgacgg cggcttcgac
      421 cgcggaagcg attttcggca gcataccgcc atacagcgta ccgtcggcaa tcagttcatc
      481 aatccgtttc ggcgtgagtt tggtcagcag attgcccgtt ttgtccatca caccggcgat
      541 attcgtcatc atcaaaagtt tttcggcgtt caattcttcc gccaatttgc ctgccaccaa
      601 atcggcgttg atgttgaacg cttcgccttt ttcacctacg ccgacggggg cgacgacggg
      661 aatgcagcca cgttctatca gccctttaac caaaccggta tcgatgcttt ccaccgtacc
      721 gacctgtccg atgtccacgc cattctgttc gggcgtatca atcaaaagtt tcttcgcctt
      781 aatgaaatgg tcgtcgcgtc cgcttacgcc gaccgcgtgt ccgccatatg tgttaatcat
      841 cgacacgatt tctttattga catgcccgcc caacaccatt tcgacaatat ccatcgcctc
      901 tttgtcggta acgcgcattc cttggacaaa ctcacccttt ttgccgactt tttcaagcat
      961 cgcattgatc tgcggcccgc cgccgtgaac gatgacggga tgaatgccga ccagcttcag
     1021 cagcacgaca tcgcgggcaa acccttcttt caaggcaggt tcggtcatcg cgttgccgcc
     1081 gtatttgatg acggcgaccg aaccggaaaa ccggcggatg taaggcagcg cttcggcaag
     1141 gatacgcgcc ttgtcggcgg cggaaataat gttttcagac tccataacgg ctccctattg
     1201 ttgacaattt cggcttatgt taacgccaaa cgccgtctga aacaatcttc agacggcatc
     1261 cggcagactt tcgggcaggt tgaagccttg cgatacgggc ggtaaaatcg cggtttatcc
     1321 ggttcgagaa accggcgaca gcaaggactt tcccatgatt aaaatcagca cccgcttcga
     1381 tgccggctcg gtcgtcgtca aagacctgac cgatccttcc aacatccgcc tcgccctgcg
     1441 tcccgacaac gcctccgatt ttgcacaatg gttctacttc cggctgcaag gcgcggcata
     1501 tcaaaactgc atcatgcatt ttgaaaacgc ggcagaagcc gcctacccga aaggctggga
     1561 aggctatcag gcgtgtgcct catacgaccg ccgcaactgg ttccgcgtac cgacttccta
     1621 cgaaaacggc gtactgaccg tcaatcatac gccgctgtcc aacagcgtgt attacgccta
     1681 tttcgaacct tactccgaag aacagcattt aaacctcctc ggcgacgcgc aaggcagcgg
     1741 cctgtgccgc atcgacgact tgggcagcac cgtgcaaggg cgcgacatca atctgctgac
     1801 catcggcaat caggtcgaaa gcgatctgaa aatctggatt accgcccgcc agcatccggg
     1861 cgaaaccatg gcggaatggt ttgtcgaggg gctgctcggc aggctgctcg atccgcaaga
     1921 cccgacagcg cgcaccctgc tcgaccgcgc cacgttctat atcgtcccca atatgaaccc
     1981 cgacggttcg gcactgggca accttcgtac caacgccgcc ggtgcgaacc tcaaccgcga
     2041 atgggaaaat ccgactttgg aaaaaagccc cgaagtgttc ttcgtgcgtg aaagaatgct
     2101 ggaaaccggc gtggatttgt ttttggatat ccacggcgac gaagggttgc cctttgtctt
     2161 tgttgcagga actgaaggcg tgccgaacta caatccgcgc atttccgcgt tggaaacaca
     2221 gttcaaaacc gcccttttga acgccagccc cgatttccaa gacgaatacg gttacgaaaa
     2281 agatgcgccc ggtcaggcaa acatgacctt ggcaacaaac tgggtcggca accgtttcaa
     2341 ctgccttgcc tatacgttgg aaatgccgtt caaagacaat gccaacctgc ccgacgacga
     2401 tttcggatgg aacggtcaac ggtcgttgcg cttgggcgaa gccgcgttgt ccgccatcct
     2461 caacatcatc ggcgatttgc gctgaaaccc ttattgcaga aatgccgtct gaagcatcat
     2521 gcctttcaga cggcatttga aaacgaaagc ccgttatgat tacccatccc caattcgacc
     2581 ccgtccttat cagtatcggc ccgcttgccg tccgctggta tgccctaagc tacatcctcg
     2641 gatttattct ttttaccttt ctcggcagaa ggcgcatcgc gcaaggcttg tccgttttta
     2701 ccaaagaatc gctcgacgac ttcctgacat ggggcatttt gggcgtaatt ttgggcgggc
     2761 gtttgggtta cgtcctgttt tacaagtttt ccgactacct cgcccatccg cttgatattt
     2821 tcaaggtatg ggaaggcgga atgtcgttcc acggcggctt tttgggtgta gttattgcca
     2881 tatggttgtt cggccgcaaa cacggcatcg gcttcctcaa actgatggac acggtcgcac
     2941 cgctcgttcc gctgggtctc gcttcgggac gtatcggcaa cttcatcaac ggcgaacttt
     3001 ggggacgcgt taccgacatc aacgcatttt gggcaatggg cttcccgcag gcgcgttacg
     3061 aagatgccga agccgccgcg cacaatccgc tttgggcaga atggctgcaa caatacggta
     3121 tgctgccgcg tcatccctcg cagctttatc agtttgcact tgaaggcatc tgcctgttcg
     3181 ccgtcgtttg gctgttctct aaaaaacagc ggccgaccgg acaagtcgcc tcgctcttcc
     3241 tcggcggcta cggcatattc cgcttcattg ccgaattcgc acgccaaccc gacgactatc
     3301 tcgggctgct gaccttgggg ctgtcgatgg ggcaatggtt gagcgtcccg atgattgttt
     3361 tgggtatcgt cggctttgtc cggttcggca tgaaaaaaca gcactgaccg cctgtgaaaa
     3421 ataccgtctg aaacatggaa atccggtttc agacggcatt tttacgtttg gttcatggaa
     3481 acaaacgctt caattcttac cgcaacacgc cttgtatttt ctgccgctgc cgcacggaca
     3541 gggatcgttc ctgcctgttt tttcgccttc cctgcggacg gtttgcggtt tgttgatgac
     3601 tgcctgccag tagcggtaga tatccgccaa tgcataaggc aactcggatt ccagttccgc
     3661 cagctcgcct tctgtgaatt gcagacggat agcgccgttt tcctcttcgt cgtaaatacc
     3721 gcccaatgcc atgatgggat aaaacaactc ttcaaacgct tcatcatcga cggcttcaaa
     3781 ccaatcggtc ggcacaatat ccaaaccgta aagataagca ttgcaccatg tgtaaaaatc
     3841 gctgccgccg tcttcgtttt catacagcca caaatcgggc agttttttat ccgacatcgc
     3901 ggcggttgtt tccatcgcca ttgccaaaac cagccgttcg atttcggaac gttcggcggc
     3961 ggtaaattgc gattcgtcgc ccaacacttc gggcagccag tcgagcggtg tcaatttgtc
     4021 cggcccgctc aacagcgccg tcataaaacc ttgaacctcg tcgcaacgca tcgtgttgcc
     4081 ttgttcgctt ttggcatcca acaattcgct caaccgccgt ttggatgctt cggtaaattt
     4141 tcgggaatcc atcattttcc ttttcaaatg ggttttgcgc cctattatag tggattaaat
     4201 ttaaatcagg acaaggcgac gaagccgcag acagtacaaa tagtacggca aggcgaggca
     4261 acgccgtact ggtttaaatt taatccacta tatcgccgca atgccgtctg aagccttttt
     4321 atgcttcaga cggcatcgtc tgttttcacg ctcaaatcgt accgctgcct tgcgctttcg
     4381 gtttggcggc actaaattcc agtttgctca aagcggaaag gtaggctttg gcagtggcaa
     4441 ccaaaacgtc ggtatccgcg ccctgaccgt tgacgacgcg gttgccgcgt gccagacgga
     4501 cgctggtttc gccttggctt tccgtacctt gcgtgacggc attgacggaa taaatctgca
     4561 aagtcgcgcc gctttgcgcc acgctttcaa tcgctttgaa aattgcatca accgggccgg
     4621 aaccggttgc ggaagcgcgt ttttcttcgc ctttgatgct gaacacgatg tcggcgcgcg
     4681 gctcttcgcc ggtttcggta ctgatttttt gggaaatgaa tttgtagctt tcggtattca
     4741 tattgcccat ttcgtccgac accagcgcgt gcaggtcttc atcgaagatt tcgcgttttt
     4801 tgtcggcgag ttctttgaag cgtgcaaatg ccacgttcag tgcctcttcg ctttccaact
     4861 cgatgcccaa atccgccagc ttggttttga aggcgttgcg gccggacaat ttgcccaagc
     4921 tcaatcggtt agccgaccaa ccgaccgatt cggcagacat aatctcgtaa gtttcgcggt
     4981 gtttcagcac cccgtcctga tggatgcccg attcatgcga aaaggcattg gcaccgacaa
     5041 tggctttgtt gggctgcacg ggatagcccg taatggtgga caccagtttg gacgaaggca
     5101 cgatttgcgt ggtatcgatg ccggtttcca agccgaacaa gtcgtggcgc actttcaacg
     5161 ccatcacgat ttcttcaacc gaagcattgc ctgcacgttc gcccaagccg ttgacagtac
     5221 attccacctg acgcgcgccg ccttttaatg cggcaagcga attggcaacc gccaagccca
     5281 aatcgttgtg gcagtgtgcc gaccaaacga ctttgccgcc gttgggcgtt ttggcaatca
     5341 gttcgcggaa aaattcttcg gttttgtacg ggatggaata gccgacggta tcgggaatat
     5401 tgatggtggt cgcgcccgct tcaatcaccg cgccgcagat ttcagcaagg aaatcgattt
     5461 ccgaacgcaa cgcgtcttcg caggaaaatt ccacatcgtc ggtgtattct ttggcgattt
     5521 tcaccgcttt gaccgccgcc tcaatcacct gcttcggctt catcttcaat ttgtactcca
     5581 tatggatggg gctggtggcg atgaaggtgt ggatgcgttt tttcggcgcg ggcgcaacgg
     5641 cctcccccgc ctgacggatg tcccgctcga tggcgcgtga caatgaacag accgttgatt
     5701 tggtaatggt tttcgcaatc gcattgaccg cctcgaaatc gcccgggctg gcagcggcaa
     5761 aacccgcttc gatgatgtcc acacccaatt tttccagctg gcgggcgacg cggatttttt
     5821 cctctttggt catagcggcg ccgggcgatt gttcgccgtc gcgcagggtg gtgtcgaaaa
     5881 tgataacgcg gttggcctgt gtcatttctg ttctctccag agattcgttt ttttgtaaaa
     5941 aagcattcaa atccagcggc ctgccttgcg attcggcaag cgcggtcagc ttttgcagtt
     6001 gcgccaaagg cagcgacccg cgcgtgcgcc atttatagat ggcggcggtc gtcgcggcat
     6061 tttcgggatc gtgctgtttc aacgcttcgg caagcgcgtt cacgccgccg aaataagcaa
     6121 ccaagcggtc tatatctaat tgcatggtag tccttgtcta aaataaatca gcctacagca
     6181 gaaatatgga caggattata cgcattttgg acaaaacggc aatataaatt tgaaaaaatc
     6241 tcaataaacc gccatagatt ttattaattt tatacatttt gtccatttta ttgcagacgc
     6301 ggcgtgattg ttgacaattg cggcagaaca gggcaaattc ggcggcacaa ggtggcaacg
     6361 agacaggaca aaccaacaag gacggaaaca atgaaataca aaagaatcgt atttaaagtc
     6421 ggcacatctt cgattaccca ttcggacggc agtctctcgc gcggcaaaat ccaaaccatc
     6481 acccgccagc ttgccgcatt gcatcatgcg ggacacgagc tggtcttggt gtcttccggc
     6541 gcggttgcgg cagggttcgg tgcgctgggt ttcaaaaaac gtccggtcaa aatcgccgac
     6601 aaacaggctt ccgccgccgt cgggcagggg ctgctgatgg aagaatatac ggcaaacctg
     6661 tcttcagacg gcatcgtgtc cgcgcaaatc ctgctcagcc gcgccgactt tgccgacaaa
     6721 cgccgctacc aaaatgccgg cggcgcactt tccgtgctgc tgcaacgccg cgccgtcccc
     6781 atcatcaatg aaaacgatac ggtttcggtt gaggaattga aaatcggcga caacgacaca
     6841 ttgagtgcgc aagtggcggc gatgatacag gcagacctct tggtgctgct gaccgacata
     6901 gacggtcttt acacgggcaa cccgaacagc aatcccgatg ccgtacggct ggacaaaatc
     6961 gaacacatca accatgaaat catcgaaatg gcgggcggat cgggttcggc aaacggcacg
     7021 ggcggtatgc tgaccaaaat caaagcggca accatcgccg ccgaatccgg cgtaccggtg
     7081 tatatctgtt cctcgctcaa acccgatgca cttgccgaag ctgccgaaca tcaggcggac
     7141 ggctcgtttt tcgtcccccg tgccaaaggt ttgcggacgc agaagcaatg gctggcgttc
     7201 tattccgaaa gccggggcag cgtttatgtg gacgaaggtg cggaacacgc tttgtccgaa
     7261 cagggaaaaa gcctgctgat gtcgggcatt gccagaatcg aagggcattt ttcccgtatg
     7321 gacaccgtaa ccgtgtacag caaggcaacc aaacagcccc tgggcaaagg gcgcgtcctg
     7381 ttcggttctg ccgccgccga agacctgctc aaatcgcgta aggcgaaagg cgtgttcatc
     7441 catcgggacg actggatttc catcacgccc gaaatacgcc tgcttctgac cgaattttag
     7501 aaaaggaaac ccatgtcaaa cacacaaaaa cagcttgccc ttgccaaagc ggcaaaaaaa
     7561 tccgtcaaca cggcggatac ggaagaaaaa aaccgcgcgc tgcttgctat ggcggacagt
     7621 ctggaagcgg cggcggcaga tattttggcg gcaaaccgtc aagacttgga agccgcggca
     7681 ggcaacattc ccgaaagcat gaccgaccgc cttttgttgg acggcaaacg catttgcgcg
     7741 atggcggacg gcattagggc ggttaccgca ctgcccgacc ccgtgggcga aatactggaa
     7801 acctcgactc tgcccaacgg cttggaaatc gtcaaaaagc gtgtggcgat gggcgttatc
     7861 ggcattattt acgaaagccg cccgaacgtt acgtccgatg cggcggcttt ggcactgaaa
     7921 agcggcagcg cggtcgtact ccgcagcggc aaagatgcat tccaatccgc acgcgccatc
     7981 gttgccgccc tgaaaacggg gttggcacaa acccgcatcg accccgacgc gctccagttg
     8041 attgaagaca ccgggcgcga aagcagttac gaaatgatga gggcgaaaga ttatctagac
     8101 ctgctgattc cgcgcggcgg ggcggggctg atacgggcgg tggttgaaaa tgccgtcgtg
     8161 ccggtcattg aaacgggaac gggcatcgtc cacatttata tcgacaagga cgcggattgg
     8221 gacaaggcgc tccgtatcgt gtacaacgcc aaaaccagcc gcccgtccgt gtgcaactcg
     8281 atggaagtgc tgctggtgca tgaagacatt gctgccgact tcctgcccaa gctcgaacgg
     8341 ctgttggttc gcgaccgcat agaagccgga ctgccgcccg tccgcttccg tttggatccg
     8401 caggcggcgc ggcatatcgg cggcgaagcg gcgggtgcag acgatttcga taccgagttt
     8461 ttagactaca tcctcgctgt gaaaaccgtc gcatcggtcg aagaggcggt cgggcacatc
     8521 gaagcccacg gcacacacca ttcagacggc atcgttaccg aaaaccgcca cgctgcggac
     8581 tatttcacga cccatatcga ttctgccgcc gtgtatgtca acgcgtccac gcgctttacc
     8641 gacggcggcg aattcggctt gggttgcgaa atgggcatct ccacgcaaaa actccacgcc
     8701 cggggcccga tgggtttgaa agagctgacg agttacaaat acatcgtaca aggcacggga
     8761 caggttaggg aataatccga attcaaacgt ctgatatagt ggattaactt taaaccgata
     8821 cggc
//
LOCUS       DAWFLT010000076         8788 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWFLT010000076 DAWFLT010000000
VERSION     DAWFLT010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8788
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.076"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(391..825)
                     /locus_tag="WGR74_001724"
     CDS             complement(391..825)
                     /locus_tag="WGR74_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ0906095.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WGR74_001725"
     CDS             complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WGR74_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ0906096.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1556..2398)
                     /locus_tag="WGR74_001726"
     CDS             complement(1556..2398)
                     /locus_tag="WGR74_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ0906097.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPVPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPGNASAKADSEQGNGE
                     DKGTGIKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2653..3291
                     /locus_tag="WGR74_001727"
     CDS             2653..3291
                     /locus_tag="WGR74_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ0906098.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3743..4543)
                     /locus_tag="WGR74_001728"
     CDS             complement(3743..4543)
                     /locus_tag="WGR74_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ0906099.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(4679..5182)
                     /locus_tag="WGR74_001729"
     CDS             complement(4679..5182)
                     /locus_tag="WGR74_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906100.1"
                     /translation="MDAFDFFMQGNFKKIATEQGLDRKYFIFQYIVCNLSAISSFCLM
                     KIIHTYTILYILIAVIGIINGFLLRSKVLSIVKNKQKFLSDMFPLEMPFFFVEYKIPI
                     LARFLNRGMKWLYLPSFFCLVAICFSIYILNEEGGWMPYFACYWFGLCYPVSTYYVFL
                     SRDYKHI"
     gene            complement(5519..6160)
                     /locus_tag="WGR74_001730"
     CDS             complement(5519..6160)
                     /locus_tag="WGR74_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ0906101.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
     gene            6489..8702
                     /locus_tag="WGR74_001731"
     CDS             6489..8702
                     /locus_tag="WGR74_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0906102.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPNNDKKLVLAARSLA
                     EAHYPADAATPYGEPLPDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVTE
                     RCNSTVCELVKGVDEVQKLTQFARVDSLATPEERARQAEIMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNVPDNPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGNPSVNWLYEGWVKSGKAISKIRAYIRQQNAD
                     TVREEGRVQIDKQLAKLTPKPNLQELAENLGFKKLDDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKTGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     ERGISVHRKTCPSFRHLAEQAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVTDLPRVLASLGDVKGVLSVTRL"
BASE COUNT         2352 a         2342 c         1899 g         2195 t
ORIGIN      
        1 ggtatagcgg attaacaaaa accagtacgg cgttgcctcg ccttaactca aagagaacga
       61 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtctacggc
      121 ttcgttgcct tgtcctgatt tttgttaatc cactataaaa attagaaatg cacattttca
      181 ttattctcgc gcaggcagga ctccagactt acccatttca gtaatgtttg aaaataaaag
      241 aaaaatcaga tgtttgtatt cccgcctgcg cagaaatgga gacggcgctc tgtcgtctca
      301 tttttgtttt aatcaactat atatagctga ttaaacataa gaaatgccgt ctgaaagact
      361 ttcagacggc attcgttcaa gcgtcgaact ttattgcgcc ttggtttcgg ttacaaaacc
      421 gattttggtg attcctgcct gacgggcggc ttctaaagct ttgtttacat aatcgtattc
      481 caccgccttg tctgccgcaa tcgccacaat cacgttttca ttctgctcct tggcggcttt
      541 cagacggctt tccacttccc cgatttccac tttgcttgca gaatccccgc cgacataata
      601 gccgccgttc gcatcaatcg tcaggcgcag ggggtcttta ggctgtttgt cctgcttgtt
      661 tgtctgctcg gacgcggtcg gcagttccaa agggatggaa tgcgtcagca ccggcatagt
      721 aatcataaac acaatcagca acaccagcat cacgtccacc aacggcgtaa cgttgatgtc
      781 ggacatcgga gaatcgtcgc cggaattcat cgaaccaaat gccataatca gctatccttt
      841 tgattaagca ggcggacgtg caaatcgtgc gccatcgcat ccaaatcctg ggtcagtatt
      901 tttgtgccgc gattgaggaa gttgtatgcc aacaccgccg gaatcgccac gaacaaaccc
      961 gccgccgtcg ccaccagtgc ctcgccaatc gggccggcaa ccgccgcaat actcatctgc
     1021 ccgctttgcc cgatattgat cagggcgtgg taaatccccc aaaccgtgcc gaacaacccg
     1081 ataaacggcg cggtcgcgcc gatggaggca agcgcggtca tcccgtaatc aaaccggcgc
     1141 ataatctgcg ccatactgtt gcggatttga atgaccaaat actcgttcaa cggcaaagcc
     1201 tgcgccagtt cggacgcttc gtttcggcgg tagttgcggt aagactgcaa tgcctcttgc
     1261 gccagtttgg acaaaggcgc atcgacggcg cgcacttttt cgaccgcgtc gttcagcgac
     1321 aaagtatcgc gcatatgccg tttgacggcg gcattccctt tgcgcgcccg atacagcttg
     1381 atgcagcgca agacaaccaa acaccacgtt acgatactca tcaacagcat caacacaaac
     1441 acaccgatca ggacgggatc gcccgattca aacactaatt tcaaattcat aatgattcca
     1501 acactgaaaa aaccaatcaa acatccaagc tgccgcaaac cgctgcggca accgcctaat
     1561 tcaattcaaa cttgacgggg actttaaact ccgtccaggc attggcttga aaatgcccgt
     1621 tttgcgccgc cttgcgtgcc gcattgtcca accgggaaaa accactgctt ttcacgattt
     1681 taacggactc aacatgaccg cccggagaaa ccaaaacgct caaaacaacc gtaccctgct
     1741 catcattctc catagaaagc gtgggataag ccgggcgcgg aatgctgccg ttggcgcgta
     1801 aaggattgcc tttgctgctg ccggctcctt ccccgtgttc gcctttgaca ccgccgctac
     1861 ctttgccgct gccttctccg cgccccgttc cgtctccttt gataccggtt cccttatctt
     1921 ccccattgcc ctgctcgctg tctgctttgg cagaagcatt gccgggatgt tcggcaggtt
     1981 tttcagacgg cttctcgacc ggtttttccg ccggtttcgg gacaggcttc gcttccggct
     2041 taggctctgg tttcggtttt tcttcgggtt tcggcttttc ttcaggtttc ggctcttcct
     2101 taggctgctg aatatccgca tccgcctttt tcgtaaccac cggcttcaaa accggcttgg
     2161 gcggctcgac aggtttgggc ggctcgggca cgggttgcgg ttcgggcgcg gcaggcgcgc
     2221 ctgcaccttc gggtgcgccg tcccctccgc caaaatcgcc caaatcgaca aattcaataa
     2281 cattgcctga ctctatcacg ggcagcttgt gcgcctgcca gagcaatgcc accattgcca
     2341 aatgcagcag tgcgacggaa aacacgactg cgggggttaa aattcgttct ttatccataa
     2401 ttcgggcata ataatagcaa caattcctat ttgcaaccta tttttacaat ttttggtcat
     2461 atgaatgtct gttccgttca caggcaaacc gtgtttaaac gctgtattac agcaaatcat
     2521 cagataacgg gccggcagaa aaaatgattc cgtctgattt cttattccaa taaaatcagg
     2581 ttagatgata tattgccgct tctgtctgtc agccgtttcg ggctgcacac cacatctgtt
     2641 caaaggaaaa ccatgtttca aaattttgat ttgggcgtgt ttctgcttgc cgtcctgccc
     2701 gtgctgctcg ccattaccgt cagggaggtg gcgcgcggct atacggcgcg ctactgggga
     2761 gacaacactg ccgaacaata cggcaggctg acactgaacc ccctgcccca tatcgatttg
     2821 gtcggcacaa tcatcgtacc gctgcttact ttgatgttta cgcccttcct gttcggctgg
     2881 gcgcgtccga ttcctatcga ttcgcgcaac ttccgcaacc cgcgccttgc ctggcgttgc
     2941 gttgccgcgt ccggcccgct gtcgaatctg gcgatggctg ttctgtgggg cgtggttttg
     3001 gtgctgactc cgtatgtcgg tggggcgtat cagatgccgt tggctcaaat ggcaaactac
     3061 ggtattctga tcaatgcgat tctgttcgcg ctcaacatca tccccatcct gccttgggac
     3121 ggcggcattt tcatcgacac cttcctgtcg gcgaaatatt cgcaagcgtt ccgcaaaatc
     3181 gaaccttatg ggacgtggat tatcctgctg cttatgctga ccggggtttt gggtgcgttt
     3241 attgcaccga ttgtgcggct ggtgattgcg tttgtgcaga tgttcgtctg actggctttc
     3301 agacggcata aacgctccag aaaacgcggc aggacatatt gccctgccgc gttttcctgt
     3361 agtgtaatct tatttttttc atcattatta gaaccaagtt gcatgataat acctttcatt
     3421 aactgaaaca ctgattaaga aactccagtc tgtctaatga tgaggttttc acatcgccaa
     3481 aacttgccaa tcaaatgctg gatttattgc cgtctgagat ttggtcaaat ccaaaggcga
     3541 cattcttaga ccctgtgtgt aaatcagggg tatttttgcg tgaaatcgtc aaacgcttgg
     3601 atgaaggctt ggccaatcaa ataccagata aacaaactcg cattaaccac attttaaaaa
     3661 atcaagtttt tggaagtact gccacgtatg taggtagctt tgaccgatat ttgcataaaa
     3721 actcctttgc tggtgaaagg aattattttg ccaattttaa aatatttctg gcaccaaata
     3781 gtacaatgac aaagacaatc atgccaatga ttaaatcagg atagctagaa tgagtcaata
     3841 acgtcaatgc tcccgccgct atcacaccga tattgatgat aatgtcattg gatgtaaaaa
     3901 tcatgctggc tttgatatgg atttctttat tttgattttt gctcagtaga tataagcaca
     3961 gccagtttgc aatcaatgcc aaaaatgccg tgccaatcat cagttgataa ttgggcagct
     4021 gctcagcacc gataaaacgc ctaatcactt ctatcacccc aaataacgcc aatattatct
     4081 gcgttatccc cgccaaaaat gccacacgtt ttttatacgc cagcgtcata ccaatggctg
     4141 atagcgccaa tatatagaca aagctgtccg ccagcatatc tagactatca gcaatcagcc
     4201 ccatagaatt agcaaaaata ccaaccgaac actctatgat aaaaaacaca aagttaatca
     4261 tgagcacttg atataataat cttttttcta agtgctcatc aggcttgtta aacactatct
     4321 tatcaacaat cacttcggtg gaaatgatat gactatcaaa attaagcggt tcaagtactt
     4381 gtaaaatcgt tgtatcttga ttatcgtgat agacggttaa gcaccgccca gcaatatcaa
     4441 actgtaattc ataaatatca gacacatctt ttaaacgcat gcgaatgagc tgttcttcgg
     4501 acgggcagtc cattttggta atgttaaaaa tggtcttttt catctattta gttccttgtt
     4561 ttgatcaggt tggctcaaat aaatctgtgt ttatattgct gcttggtaat ttttggatgg
     4621 tttgagtaaa ttgattaggt taaaatttac ctttggaagt accgccacgc ataatagttt
     4681 agatatgttt ataatctctg gataaaaaaa cgtaataagt gcttactgga taacaaagtc
     4741 caaaccaata gcaggcaaaa taaggcatcc accccccttc ttcattaagg atatatattg
     4801 agaaacaaat cgcaactaaa cagaaaaaac ttgggagata aagccatttc attcccctat
     4861 tcaagaatct agccaagata ggtattttgt attctacaaa aaagaaaggc atttccaagg
     4921 gaaacatgtc agataaaaac ttttgtttat tttttactat agatagaacc ttgcttctca
     4981 agagaaagcc attaataata ccgatgacag ctattaatat atagagaata gtataagtat
     5041 gaataatctt cattagacaa aaagaagaaa tggcagataa attacatacg atatattgga
     5101 atataaaata tttacggtct aaaccttgtt cagttgcaat ttttttaaaa ttgccttgca
     5161 taaaaaaatc aaaggcgtcc attaaactat ctttcacatt agaaatttaa aagctaaata
     5221 atacgacaaa caatgtgaag tactattcat ggtttattta aaaaataata ctattctgaa
     5281 cattatttag atacagaaat taacaaatta gaactaaaca agcttttaaa tactttaatt
     5341 ttattggaaa gctataaaag gaactataac tttatacact agtcacttct ttttaagagg
     5401 caaaagggat tgggaaggtc gtcttggaga taagcactgg tatttcggcc aatggtaaat
     5461 agagtttacc tcaaataggg tagaacctcc ttcatctgtc agttaataac agccactttt
     5521 acaatgccct gtcgaaataa agtggcacgc ccgatttttc actcataccg attaaataat
     5581 ccatcgtctt ccgagggaac gcgatgccgc gcaccacggt cagattcctc aaaacgggga
     5641 aaaccaaaat atcctccata ccgattccgc cgttgatgcc gtctgaagca ccgtccatca
     5701 aattttccaa ctcttgcaaa tctgcgttta tccgttcgag gtattgggcg gttttattca
     5761 aattggcgga aaagctgccg atgcttttct cttttttgtc tgtaaaatat ttcaccgctt
     5821 ccggcgttgc aaattcaggc agcccgattt tgatcacgcg cggctgcacc agtttgtcgt
     5881 tgtatccgcc caccttgtcc agccacgccc gtatctcggg gcggacttcg tctttcagac
     5941 ggtcttcgcg gtcgaaatgc cgcacaatgt ccaaactctc gcccataaac gaaccgtctt
     6001 ctttttgcag gacgggcact tgtttcgcac cgatcatacc gatcggcgtt gcctcgtcgt
     6061 cgtttgccag cacggcttct tcaacgtccg cgccaaacaa cccggcagcc atccgcgcac
     6121 gcacgcaaaa cgggcaatgg tcgtaaatat acagtttcat caaaatattc ctcgtcaacc
     6181 tgtcggtacc ggctacctta acaccccgcg ccacccgaaa caagtttatc ttcccgccta
     6241 tgcaccgtaa ataaataagc tgagaccttt gcaaaaatag tctgttaacg aaatttgacg
     6301 cataaaaatg cgccaaaaaa ttttcaattg cctaaaacct tcctaatatt gagcaaaaag
     6361 caggaaaaat cagaaaagtt ttgcattttg aaaatgagat tgagcataaa attttagtaa
     6421 cctatgttat tgcaaaggtc tcaagctgtt acaataaact cgtttttatc ggaacggaag
     6481 accccatcat gaccgccatc agcccgattc aagacacgca aagcgcgact ctgcaagaat
     6541 tgcgcgaatg gttcgacagc tactgcgccg cgctgccgaa caacgataaa aaacttgtct
     6601 tagccgcccg ttcgctggcg gaagcacatt accccgccga tgccgccacg ccgtatggcg
     6661 agccgctgcc cgaccacttc ctcggcgcgg cgcaaatggt tcatgaactc gacctgctcc
     6721 ccgatgccgt cgccgccacc ctgcttgccg acatcggacg ctacgtcccc gactggaacc
     6781 tattggttac cgaacgctgc aacagcaccg tttgcgagct ggtcaaaggc gtggacgaag
     6841 tacagaaact gacccaattc gcacgcgtgg acagccttgc cacgccggaa gaacgcgccc
     6901 ggcaagccga aattatgcgg aaaatgctac tggcgatggt taccgacatc cgcgtcgtgt
     6961 tgatcaaact ggcgatgcgt acgcgcaccc tgcaattttt aagcaacgtt cccgataatc
     7021 ctgaaaaacg cgccgtcgcc aaagaaaccc tcgacatctt cgccccgctc gccaaccgtt
     7081 tgggcgtgtg gcaactcaaa tggcagctcg aagatttggg cttccgtcat caagagccgg
     7141 aaaaataccg cgaaatcgcc ctgcttttgg acgaaaaacg caccgaacgc ctcgaataca
     7201 tcgaaaactt cctcaatatc ctgcgtacag aactgaaaaa atacaatatt cactttgaag
     7261 ttgccggaag accaaagcac atctactcca tttacaaaaa aatggtgaag aaaaagctca
     7321 gcttcgacgg cttgttcgac atccgcgccg tgcggattct ggtcgatacc gtccccgagt
     7381 gttacaccac gttgggcatc gtccacagcc tctggcagcc catccccggc gagttcgacg
     7441 actatatcgc caacccgaaa ggcaacggct ataaaagttt gcacaccgtc atcgtcggcc
     7501 ctgaagacaa aggcgtggaa gtacaaatcc gcaccttcga tatgcaccaa ttcaacgaat
     7561 tcggtgtcgc cgcgcactgg cgttacaaag aaggcggcaa aggcgattcc gcctacgagc
     7621 aaaaaatcgc ctggttgcgc caacttttgg actggcgcga aaatatggcg gaaagcggca
     7681 aagaagacct cgccgccgcc ttcaaaaccg agctgttcaa cgacacaatt tacgttttga
     7741 ccccgcacgg caaagtcctc tccctgccta cgggcgcgac ccccatcgac ttcgcctacg
     7801 ccctgcacag cagcatcggc gaccgctgcc ggggcgcgaa agtcgaagga caaatcgtgc
     7861 cgctgtccac tccgctcgaa aacggacagc gcgttgaaat cattaccgcc aaagagggga
     7921 atccttccgt caactggctc tacgaaggct gggtcaaatc cggcaaggca atcagtaaaa
     7981 tccgcgccta catccgccag caaaacgccg acaccgtgcg cgaagaaggc cgcgtccaaa
     8041 tcgacaagca gcttgccaaa ctcacgccca agcccaacct gcaagagctt gccgaaaacc
     8101 tcggcttcaa aaaactcgac gacctctaca ctgccgtcgg acaaggcgaa atttccaacc
     8161 gcgccatcca aaaagcctgc ggcacgctga acgaaccgcc gcccgtaccc gtcagcgaaa
     8221 ccactatcgt caaacagtcc aaaatcaaaa aaggaggcaa aaccggcgtg ctcatcgacg
     8281 gcgaagacgg actgatgacc acgcttgcca aatgctgcaa acccgcgccg cccgacgata
     8341 ttgtcggctt cgttacccgc gaacgcggca tttcggtaca ccgcaaaacc tgcccctctt
     8401 tccgacacct cgccgaacaa gcacctgaaa aagtgctgga cgcaagctgg gcggcgttgc
     8461 aggaaggaca agtgttcgcc gtcgatatcg aaatccgcgc ccaagaccgc tccgggcttt
     8521 tgcgcgacgt atccgacgca ctcgcccgcc acaaactcaa cgttaccgcc gtgcaaaccc
     8581 agtcccgcga cttggaagcc agcatgaggt tcacgctcga agtcaaacaa gttaccgacc
     8641 tcccgcgcgt cctcgccagc ctcggcgacg tcaaaggcgt attgagcgtt acccggcttt
     8701 aaatacaaaa atgccgtctg aaagccgtat aacgcttcag acggcatttt gattaccagg
     8761 gtttgctatt ttttgttgca tagtcaat
//
LOCUS       DAWFLT010000077         8565 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWFLT010000077 DAWFLT010000000
VERSION     DAWFLT010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8565
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.077"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(80..598)
                     /locus_tag="WGR74_001732"
     CDS             complement(80..598)
                     /locus_tag="WGR74_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ0906103.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(618..2003)
                     /locus_tag="WGR74_001733"
     CDS             complement(618..2003)
                     /locus_tag="WGR74_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ0906104.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKFVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            2242..3783
                     /locus_tag="WGR74_001734"
     CDS             2242..3783
                     /locus_tag="WGR74_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ0906105.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFLDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVYAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLAAFAVCIFLLLDGGEGGK
                     RTETL"
     gene            3812..4411
                     /locus_tag="WGR74_001735"
     CDS             3812..4411
                     /locus_tag="WGR74_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ0906106.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            4408..5013
                     /gene="nadD"
                     /locus_tag="WGR74_001736"
     CDS             4408..5013
                     /gene="nadD"
                     /locus_tag="WGR74_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ0906107.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLYQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLAGQGVSDGIPPAAARYIREHGLYEK"
     gene            5072..5458
                     /gene="rsfS"
                     /locus_tag="WGR74_001737"
     CDS             5072..5458
                     /gene="rsfS"
                     /locus_tag="WGR74_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ0906108.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            5512..5982
                     /gene="rlmH"
                     /locus_tag="WGR74_001738"
     CDS             5512..5982
                     /gene="rlmH"
                     /locus_tag="WGR74_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ0906109.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWQQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE"
     gene            6055..6321
                     /locus_tag="WGR74_001739"
     CDS             6055..6321
                     /locus_tag="WGR74_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ0906110.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWMAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            6478..7161
                     /locus_tag="WGR74_001740"
     CDS             6478..7161
                     /locus_tag="WGR74_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ0906111.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            7285..7797
                     /gene="coaD"
                     /locus_tag="WGR74_001741"
     CDS             7285..7797
                     /gene="coaD"
                     /locus_tag="WGR74_001741"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ0906112.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            7829..8482
                     /locus_tag="WGR74_001742"
     CDS             7829..8482
                     /locus_tag="WGR74_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ0906113.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNKESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRCGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
BASE COUNT         1862 a         2273 c         2504 g         1926 t
ORIGIN      
        1 atcaatgtaa tccattctgt tcccgatgtt tatgccgtct gaaacccatc ctgtgtcggg
       61 cttcagacgg catattgctt caaagcaggt tttccgaggc aacccagttc agaatatcgg
      121 cttcgaccgc ttcgggcgtg ccggggttgg cgattttgac cccgtattcg ccttcgccca
      181 gttcctcgag gatttccagt tgcgaatcga gcatccctgc tttcatgtaa tgtcctttgc
      241 gcgacatcat gcgctcgagg ttgatgtctt gcggcggact gaggtggatg aaggcagctt
      301 tgccttcggc tccgcgcaga atgtcgcggt agccgcgttt gagggcggaa caggttacga
      361 tggtgtggtt cgcaccgttt tgcgcctgtt gcgtcatcca gtcgcgcaga ttgcccaacc
      421 acggatagcg gtcttcatcg gtcagcggaa tacccgcgcc catcttgtcg cggttggctt
      481 gggtgtggaa ctcgtcgcct tcggcatagg gacattgacc gaggtgtttc tgcagggaca
      541 gcgcggcggt ggtcttgccg cagccgcata cgcccataac gacaaaatgc gtagtcattt
      601 actatccttt ccgtctgtca gacgatggcg aacagcagtg cggacaaggc aaagccgatg
      661 agtgcgatga gggtttggtt gaccgtccag gttttcagcg tggtcggtac gtccatgtcc
      721 aagagacggc cgaccagcca gaagccggag tcgttgaagt ggctgcaacc gaccgaacct
      781 gccgccgttg ccaatacgat acaggcgagc tgccagtcgg taaagccggc ggcggcaacg
      841 gcaggagcca tcagcgcggc ggcggtggtc agggcgacgg ttgccgaacc ttgcgcgata
      901 cgcagtgcca aggcgacaag gaaacagccc aaaaggacgg gaatgcccaa atccgccatg
      961 ctgtcggcga gtgccttgcc gatgccggaa gcgcgcaaaa cgccgccgaa cataccgccc
     1021 gcgccggtaa tcagaatcac ggaacagacg ggggcgagtg cgccgtccac ggttttttcc
     1081 aacgcgctgc cgctttcgcc gcgtttgcgt cccaagacaa acagtgcgac caatacggaa
     1141 atcagaaggg cgatcggtgt cgaaccgatt atttttgccg tctgaaccca ggtttcgtcc
     1201 gcacttacga atttttcgct gatgagggcc gatacgccgg tattcaggaa aatcagcagc
     1261 atgggaatca gcatgatggc gacgaccgtt cctgctttgg caggttcttt cggcaggtcg
     1321 ttgtcttgcg tgccgccgct gagcagttcg ggaacgggaa catggatggt gcgccccaac
     1381 actttgccga gcatatagcc gctgaaatac catgtgatga aggcggtcgg cagacccaaa
     1441 atcaaaactt ggccgatgtt cgcgccgtaa aattcggaag cggcaatcgg gcccggatgg
     1501 ggcggcagga agacgtgcat gacggaaaat gcgccgatgg aggcaagcgc gaagggcagt
     1561 acgtcctgtt tcatgcgccg tgcggtggcg aacacgatgg gcagcatgac gattagtccg
     1621 gcatcgaaga aaatcgggaa gccgaaaatc agcgaggcaa cgcccagcgc gaacggtgcg
     1681 cgtttttcgc cgaacatccg gatcagcgcg tccgccagcg actgtgcgcc gccggatgtt
     1741 tcgaccagac gtccgagcat cgcgcccagg ccgaccagaa gcgccacgcc gccgagcgtg
     1801 ccgccgaagt ttttgaccag tacgtcgttg acaatgctgc ctgtgggcaa accggttgcc
     1861 aaagccgtca gcaggctgac gatgaccagt gtcagcagcg cgtggatgcg gaatttgacg
     1921 attaaaatca gaatgaggat gattgccgcc gccgaaatgc ccaacagggt ttgcgcggac
     1981 agcgtctgtg tccagccgtc catttgtaaa gcctttctct gtaataaaag tacaaaattg
     2041 tcaggttttt ttaagttgtg cgaaaacgat agcacaaatc gggcggtaag ttgtttgtct
     2101 gaagttatat tcctgtttat ttgaacgatt tatttcagac ggcgggagga aggcggatac
     2161 ggtatgtagt cggattgaaa tatacggtat gtggggcttt tgggtacaat cgcaacatca
     2221 gtgtttcaag tggggaaggc gatggatgga cggcgttggg cggtatgggg tgcttttgcc
     2281 ctgctgcctt cggctttttt ggcggtaatg gtcgttgcgc ctttgtgggc ggtggcggcg
     2341 tatgacggtt tggcgtggcg cgcggtgctg tcggatgcct atatgctcaa acgtttggcg
     2401 tggacggtat ttcaggcagc ggcaacctgt gtgctggtgc tgcctttggg cgtgcctgtc
     2461 gcgtgggtgc tggcgcggct ggcgtttccg gggcgggctt tggtgctgcg cctgctgatg
     2521 ctgccttttg tgatgcccac gttggtggcg ggcgtgggcg tgctggctct gttcggggcg
     2581 gacggcctgt tgtggcgcgg acggcaggat acgccgtatc tgttgttgta cggcaatgtg
     2641 tttttcaacc ttcctgtgtt ggtcagggcg gcgtatcagg ggtttgtgca agtgcctgcg
     2701 gcacggcttc agacggcacg gacgttgggc gcgggggcgt ggcggcggtt tttggacatt
     2761 gaaatgcccg ttttgcgccc gtggcttgcc ggcggcgtgt gccttgtctt cctgtattgt
     2821 ttttcggggt tcgggctggc attgctgctg ggcggcagcc gttatgccac ggtcgaagtg
     2881 gaaatttacc agttggtcat gttcgaactc gatatggcgg ttgcttcggt gctggtgtgg
     2941 ctggtgttgg gggtaacggc ggcggcaggg ttgctgtatg cgtggttcgg caggcgcgcg
     3001 gtttcggata aggcggtttc ccctgtgatg ccgtcgccgc cgcagtcggt cggggaatat
     3061 gtgctgctgg cgtttgcggc ggcggtgttg tctgtgtgct gcctgtttcc tttgttggca
     3121 attgttgtga aagcgtggtc ggccggcgaa tcgtggcgtg tgttaatgga aagtgaaacg
     3181 tggcaggcgg tgtggaatac tttgcgcttc tcggcggcgg cggtgtatgc ggcggcggtt
     3241 ttgggtgtgg tgtatgcggc ggcggcgcgg cggtcggcgt ggatgcgcgg gctgatgttt
     3301 ttgccgttta tggtgtcgcc ggtttgtgtt tcggcgggcg tgctgctgct ttatccgcag
     3361 tggacggctt cgttgccgct gctgctggcg atgtatgcgc tgctggcgta tccgtttgtg
     3421 gcaaaagatg ttttatcagc ctgggatgca ctgccgccgg attacggcag ggcggcggcg
     3481 ggtttgggtg caaacggctt tcagacggca tgccgcatca cgttccccct cttgaaaccg
     3541 gcgttgcggc gcggtctgac tttggcggcg gcaacctgcg tgggcgaatt tgcggcaacc
     3601 ttgttcctgt cgcgtcccga gtggcagacg ctgacgactt tgatttatgc ctatttggga
     3661 cgcgcgggtg aggataatta cgcgcgggcg atggtgctga cattgctgtt ggcggcgttc
     3721 gcggtgtgca ttttcctgct gttggacggc ggcgaaggcg gaaaacggac ggaaacgtta
     3781 taatgcgaac cctttttcag aggacggcaa aatgagcgag ttggataaca tccttgccca
     3841 caaccggcag tttgtcgaat cgggcgaata tgaaaaatac tttaccgaca aataccccga
     3901 acgcgggctg gcggttttgt cctgtatgga tgcgcggatt atcgggctgc tgcccgacgc
     3961 gttgggtttg aaaaacggcg atgccaagct gattaaaaat gccggcgcgc tggttacgca
     4021 cccgtggggt tcggtgatgc ggagcctttt ggttgccgtg tttgaattga aggtcagaga
     4081 gattatggtc atcgcccatc acgattgcgg tatgcagggg ctgaatgccg aagaattcct
     4141 cgggcgcgtc cgggaaagcc ggattcccga agaccgtatc gaaaccctgc gttatgccgg
     4201 tatcgacctc gacggctggc tgaccggttt cgacaacgtc gaagacagcg tgcgccacac
     4261 ggtggacctt atccgtaacc atccgctgat gccgcgccat attgccgttc acggattggt
     4321 catccatccc gttaccggca aactgacgct ggttgtggac ggcagtgttt cagacggcat
     4381 ggacttatcg gaaggaatgg aaacatcatg aagaaaatcg gactgttcgg cggcactttc
     4441 gacccgatac acaacgggca tctccacatc gcccgcgcct ttgccgacga aatcggcttg
     4501 gacgcggttg ttttcctgcc gacaggcggc ccgtatcaca aagacgcagc ctccgcttcc
     4561 gccgccgacc gccttgccat ggtcgaactg gcgacggcag aagacgcgcg ttttgccgtc
     4621 agcgattgcg acatcgtccg agaaggtgca acctatactt ttgataccgt ccaaatcttc
     4681 cgccagcagt tcccatccgc gcaactctgg tggctgatgg gcagcgacag cctgatgaag
     4741 ctgcacacat ggaaaaaatg gcagatgctc gtgcgcgaaa ccaatatcgc cgtcgccatg
     4801 cggcagggcg acagtctgta ccaaaccccg cgcgaactgc acgcgtggct gggcaagtcc
     4861 cttcaggacg gcagcgtccg catcttgtcc gccccgatgc ataatgtgtc gtcaacggaa
     4921 atccgccgca accttgccgg ccaaggcgtt tcagacggca tcccgcctgc cgccgcacgc
     4981 tacatccgcg aacacggttt gtatgaaaaa taaagtcaaa tcagtaagaa acggctataa
     5041 tgccgtctga aaacatcccg ttaggaacat aatgaacgaa caagaactgc aagacctgca
     5101 aaaaatggtc ggggtcgccg tcaacgccct cgaagacatc aaagccaaag acatttccgt
     5161 tctcgaaacg caagacaaaa cttcgctgtt tgccagaatg attatcgcca gcggcgacag
     5221 tacgcgccaa gtcaaagcac tggccaacaa cgttgccgtc gatttgaaag aagccggttt
     5281 tgaaatcctc agcaccgaag gcgacagcgg cgaatggacg ctcgttgatg caggagacct
     5341 cgtcgtccac gttatgctcc ccgccgtgcg cgacttctat gatatcgaca ccctttgggg
     5401 cggcgagaaa ccgagttttc acgccggaat gcagaagccg tggcacgcgg cagactgatt
     5461 cccgatgccg tctgaatgtt cagacggcat tttcctgtaa gggagaaagc attgaacatc
     5521 accgttttgg cagtcggcac caaaatgccg cgctgggttg atgaggccgt cgccgaatac
     5581 gccaaacgct tcggacgcga cgtcgcctac gcactcaaag aaatcaaacc cgaaaaacgc
     5641 ggcgcgggcg tgaatgccgc ccaaggtatg gcggcggaag aaaaacgcat ccttgaagcc
     5701 attccgcaag gcgcgttcct cgtcgttctt gacgaacgcg gcaaagcacc gacctccgtc
     5761 gagctggcgg aacacctcaa aagctggcag caaaacggcg aacacgtctg cttcgtcatc
     5821 ggcggcgcgg acggtatgac cgaccgcctc aaacagcagg cacgcatgat gatgcgcctg
     5881 tccagcctga ccctgccgca cgggatggtg cgcgtccttc tgaccgagca gctctaccgc
     5941 gccgcctcca tcctgcacaa ccatccctat catcgggaat aagaaggctt ttgtccgtat
     6001 ccctaatcgg ttaaaatcac cccgttattt ctgaacacag caaaggaatc cgctatggca
     6061 cgaatggtat tctgcgtcaa gctcaacaaa gaagccgaag gcatgaaatt tccaccgctt
     6121 cccaacgaat tgggcaagcg catttttgaa aacgtatcgc aagaagcatg gatggcgtgg
     6181 acgcgccacc aaacgatgct gattaacgaa aaccgcttaa gccttgccga tccgcgcgcg
     6241 cgcgaatacc tagctcagca gatggagcag tatttcttcg gcgacggcgc ggatgccgtg
     6301 cagggatacg ttccgcaata acggttttcc gttttgaaca caggctgtcc gaacctgctt
     6361 cagacggcct ttaaaatacg ccggacaact ttatcttcac gcaaccggac aaattttgac
     6421 attgggcaat gttaaaatcc cccattattc ccaaccgcgc tttcaggagc agatgatatg
     6481 caacacgacg tttacgacta caccgcgcat acggtttcta aaaacaccgt cctgcagaaa
     6541 acctaccgcc tgctcggatt ttcattcatt ccggctgccg caggcgcggc acttgccgcc
     6601 aatgccggtt tcaattttta cgccgccttc ggttcgcgct ggatagggtt tgccgtcgtg
     6661 ttggcgtttt tctacggtat gatccacttc atcgagaaaa accgttacag caataccggc
     6721 gttaccctgc tgatggtatt cacattcggt atgggcgtat tgatcggccc cgtgctgcaa
     6781 tacgcgctcc atattgccga cggtgcgaaa atcgtcggca ttgccgccgc gatgaccgcc
     6841 gccgtctttt taacgatgtc cgccttggcg cgccgaaccc ggctcgatat gaacgcgctc
     6901 ggacgcttcc tgaccgtagg tgcggtcatt ctgatggtcg ccgtggttgc caatctgttt
     6961 ttgggtattc ccgcactcgc cctgaccatt tccgccggtt ttgtcttgtt cagttcctta
     7021 atgattatgt ggcaggtacg caccgtcatc gacggcggcg aagacagcca tatcagcgcg
     7081 gcactgacac tgtttatctc gctttacaac atcttcagca gcctgctgaa catcctgttg
     7141 tctttaaacg gcgaggattg atgccggtaa aacgccgtcc gactatgccg tctgaaaatg
     7201 cttcagacgg cattttactt tgggcataca atacggatgc acacgcacca acacacagcc
     7261 gactatgaag ccgggaggaa tcaaatgtta ccgaatacac cgcgccgcgc cgtttatgcc
     7321 ggcagtttcg atccgcccac attggggcat ctgtggatga tacggcaggc gcaatctatg
     7381 tttgacgaac tcatcgtcgc catcggcatt aaccccgaca aacgcagcac ctataccgtc
     7441 gctgaaaggc aggatatgtt gtgcgatatt actaaaatgt ttcccaacgt cagaaccgat
     7501 gtatttgaaa accgatttct ggtgcattat gcccgtgagg tagatgcagg attcatcgtg
     7561 cgcggcatcc gttctgcttc ggattacgaa tacgaacgtt ccatgcgcca catcaacagc
     7621 gacctcgccc ccgaaatatc caccgtattc ctcatgccgc cgcgcgaaat cgccgaagtg
     7681 tcgtccacta tggtcaaagg actggtcggg ccggaaggct ggacggaaac gatccaccgc
     7741 tacgtgccgc aagctgtgta cgaaaaaatc cttgccgaac atcaacacga aaattgaacc
     7801 cttctatttt tgcaaggcat atatggatat gttggaaatt ttgttccgcc atcaagactt
     7861 cgtcgccatc aacaaaccgg gcggcatatc cgtccaccaa gacagcggcg agaccggact
     7921 ggcccgaaca ctcgccatac agttgggcgt agagcgtgtg tggctgctgc accgattgga
     7981 caaacagacc agcggcattt tactgtttgc cctcaacaaa gaaagcgcgt ccgcccttag
     8041 cggacagttt gccggcaaaa gcatcaaaaa aacctatttg gcactgtccg accgcaagcc
     8101 gtccaaaaag cagggttgga ttaaaggcgg aatggaaaaa tccagatgtg gaatgtggaa
     8161 gttgacgcgc aatacagaaa atatcgccgt tacccgattc cacagcatca gtatcgccga
     8221 aaaactgcgg ctgttcatcc tggaaccgca tacgggcaaa acgcaccaat tgagagtggc
     8281 gatgaaaagt ttgggcagtc cgattctggg cgacagctta tacggcggaa cagaatccga
     8341 aaccatgttt ctgtatgcat ggaaaataca atttgcctac caaaaccgac aaatcgaaat
     8401 tgttgcaccc ttaaaaaacg aatggcagac tgaaaatatc tctcatgcgc tggaagaatt
     8461 ttatatggca aaagcaggtt aataaatata aattaattta taaacaatat gataaatatt
     8521 tttataaaaa tttttcaaga aaacggttga ctgcaaatct cttca
//
LOCUS       DAWFLT010000078         8407 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWFLT010000078 DAWFLT010000000
VERSION     DAWFLT010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.078"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(288..803)
                     /locus_tag="WGR74_001743"
                     /pseudo
     CDS             complement(288..803)
                     /locus_tag="WGR74_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1811..2581)
                     /gene="xth"
                     /locus_tag="WGR74_001744"
     CDS             complement(1811..2581)
                     /gene="xth"
                     /locus_tag="WGR74_001744"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0906114.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFKNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAEALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(2641..2922)
                     /locus_tag="WGR74_001745"
     CDS             complement(2641..2922)
                     /locus_tag="WGR74_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0906115.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSGHLNRLRAGGLVDFTRYHRIIEYRLISEDAATILRTIRDLENKRAV"
     gene            complement(3014..3300)
                     /locus_tag="WGR74_001746"
                     /pseudo
     CDS             complement(3014..3300)
                     /locus_tag="WGR74_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3328..4209)
                     /gene="nadC"
                     /locus_tag="WGR74_001747"
     CDS             complement(3328..4209)
                     /gene="nadC"
                     /locus_tag="WGR74_001747"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ0906116.1"
                     /translation="MPSENTLFPLPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQVVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
     gene            complement(4284..5222)
                     /locus_tag="WGR74_001748"
     CDS             complement(4284..5222)
                     /locus_tag="WGR74_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906117.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVGRLRIWANSADTEEVRQKRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIAEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
     gene            5529..6641
                     /gene="nadA"
                     /locus_tag="WGR74_001749"
     CDS             5529..6641
                     /gene="nadA"
                     /locus_tag="WGR74_001749"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ0906118.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETAGRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEASTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKTEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKKKDVFNGMGPA"
     gene            6693..8201
                     /gene="nadB"
                     /locus_tag="WGR74_001750"
     CDS             6693..8201
                     /gene="nadB"
                     /locus_tag="WGR74_001750"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ0906119.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDQ
                     NNNGLHLTREGGHTCRRIAHVADYTGEAVMQSLIAQIRRRPNIRVCERQMALDIQTES
                     GAACGLTVLNRQTQETYRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQAGERFMPHYDRRAELAPR
                     DIVARAIAAETAKQTQDFVSLDISHQPAAFVRQHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPSGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQTVPSKRSSENTSAEADIFSDGIQNTFSRPTLQAFNQRHLGILRNDTGLRR
                     AIAQLRLWKQNQTEPHTASEYENRNLLECSLAVAQAAYARRQNIGAHFNSDC"
BASE COUNT         1948 a         2311 c         2234 g         1914 t
ORIGIN      
        1 aaataactga aaccgaacag actagattcc cgcctgcgcg ggaatgatga attcatccgc
       61 acggaaacct gcaccacgtc attcccacga acctacattc tgtcattccc acgaaagtgg
      121 gaatccagga ttcagggacg gagaaacctt ttttcccgat aagtttccgc accgaccggt
      181 ctagattccc gcctgcgcgg gaatgacggt tcgggtattt ctgacgattc atcgagccgt
      241 agcaactgta tttttcaccc cgtcgggcaa aaataccaaa actcaaatca agccgtccgg
      301 ataccgtttt cggcggtatc gttttcggca aaataatcac gcatccgggc attcgatatc
      361 gtcagcagtt tgcgcataca tgccgtaacg gcaaccttat acggcttacc ctcggacagc
      421 aggcgttggt agaaatcccg aataagcggt tcaaaacgtg tcgctgccac ggtagccata
      481 tacagtgcct tacgcaccgc agaccttccg ccaaagcagc ggcttttaaa tttggtttcc
      541 ccgccctccc tcgggtgcgg ggcaatgccg gccaaactcg ctatccgttt gtgcgacagc
      601 cgccccaatt cgggcagcat cgccatcagc gtagccgtcg ttatcgaacc gatgcctttg
      661 atttgctccg ccacttgggc tttgccgtca aaatgcgtgt gggtgtggtc gtcgatttgt
      721 ttgtccaatt cgtcaatcag ccggtcaaaa tgggcaatca gttgtttgac gcttccgact
      781 tgcgtttcat gaaactgatg cagatatagt ggattaacaa aaatcaggac aaggcgacga
      841 agccgcagac agtacaaata gtacggcaac gctgtactgg tttaaattta atccactata
      901 ttttgtaata gctgccatta tgcctactag gatatatacc ttctatataa tccttattta
      961 aataaaaacc cttataaaaa tctgtgttat ctgtcgggat tgaaaaaaag attttatctt
     1021 tctcatcagg tttatatttg ctatcaatat caaaaagaga ataaggatta caatgagttc
     1081 gacatactag gtaaaaattt atatcttgga tcatgaaata ttcgatattt atcaggatcg
     1141 aaagatgcta aaaattcatc aatatcggta ctggatattt tgtcatcact aataaacatc
     1201 gctagagatg ggcgactttg attttccatt tgttgattag taaaggatat aaatactaac
     1261 ttgccattgt caaaccacat agatttatat agtggattaa atttaaacca gtacgtcgtt
     1321 gcctcgcctt gccgtactat ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg
     1381 ttaatccact atagttattg cctttgggag aatcaattct gacagcacat aacataggtt
     1441 catctttaca gtcgttatat tgggtttcat caaactcaac tgcatagcac aataaaggca
     1501 ataggaaaaa aaggagatta atttattact atacataagg cagtctgact attcattcta
     1561 aaataaatcc ggtttttaat atttgattct gctccggatt atatacataa taaattttac
     1621 tgctccaaaa tctgatatta accctatgga tttcataaat ttttcagaaa aataggctgt
     1681 gcttgaacaa tccgccgact ccggctgttt gtcagggaaa aggcaattat tttcggttag
     1741 tcagcagcct atcctattga atcacttaaa gatatggatg tttgtcaacg ccatatcaaa
     1801 acacggtctt ttaccaatcg aattctgccg tcaccggcgc gtggtcgctc ggacgctcca
     1861 gcgcgcgcgt ctccaaatcg acacggacat ccttcaacgc ctccgccatc gcaggcgaca
     1921 ccaaaatatg gtcgatacgc aggcccagtt tgcgttggaa catcgcgccg cgatagtcga
     1981 accaggtata gaacgcgcct tcgggatgga cttggcgcag gctgtcggtc agtcccaaat
     2041 ccagcaggtt tttgaaccac tgccgttcga cggacgaaca gtggattttt tcgtgccatt
     2101 tttcagggtc gtaacagtcc gcatcggcag gcgcgatatt gaaatcaccc agcagcacca
     2161 gtttgccgta gcgggtcatt tcatcgcgga caaactccgt cagtgcggca aaccattgtt
     2221 ctttatattt gaatttcggg ctgtcgaggg cttcgccgtt gacgcaatag acattgatga
     2281 cgcgcacacc gccgacggtt gccgcaatca cgcgccgttg cggatcgtcc ggcagcgcgg
     2341 gcagcccgat atgcacgtct tccggcgcgt tgcggctgac gattgccacg ccgttgtagg
     2401 ttttctgccc gctccaaaca cagtgccagc ccatcatttg caaagcggcg gccggaaatt
     2461 tgtcctgatc gagtttgagt tcctgcaaaa ccaaaatatc gggcggattg tcggcaagca
     2521 ggttttgcac ctgcggcagc cgcacattga gcgaattgac gttccaagtg gtgattttca
     2581 taacatttcc gaaaaatgcc gtctgaaacg ttcagacggc aaaaggaaag gattttaaca
     2641 ctataccgcg cgtttgtttt ccaaatcgcg gattgtccgc agaatcgttg ccgcgtcttc
     2701 ggaaatcagg cggtattcga taatgcggtg gtaacgcgta aagtccacca gaccgcccgc
     2761 cctcaggcgg ttcaaatggc cggaaacggc ggtggccggc agggataagg attttgccag
     2821 ttcggcgata ttgcgttcgc tgtccaacag ttggatcagt atcgccatac gttcgggatg
     2881 cgccataagt ttgagtatgg tgtggagcgg tattgtgttc atcggtattc ctaaaacctt
     2941 tgccgcccgc caagcggatt gacgggcgac agatatggat gattgtgatt atctttataa
     3001 atcttattat aaatcaaata tttatggatg attgtacagg attgtatgtt ccttttccca
     3061 ctttttttgc aaacacgcgt aaaggcgggc tttcagacgg cataaaactg ccgcccccgt
     3121 cctttcccct gccctaccga tgcctccgcg ctgtcgggca aacaaaatac ctgcctgaaa
     3181 cgctgttttt tctaaagcat cacgaccttc acgaaaaatg ccgtctgaag aatatttcag
     3241 acggcatttc ctccttgagg caataaccgg attacggtta ggcggttcct tgtctgacat
     3301 atcagacaac tgccgacacc ctaaaactca tgccaagaaa tccaaaccta tgtccaacga
     3361 gcggctgctg tgggtcagat agccgagggc gatgccgtcc acgccggttt gcgccacgcg
     3421 cttcaggcgg tcgaagccga tgccgcccga tgcttcgcaa tagacggtgt gggggtgggc
     3481 ggtttgcgtg tggcagcggt ttgccgcttc tttcagggtt tcgtcgtcca tgttgtccag
     3541 caaaatccgt tccgcgcccg ctgcgatggc ttcgtccagt tgtgccaacg tgtccacttc
     3601 gatttccacg caggtcaatg ctccgaccgc ctgttttgcc tgccgaacgg cttgggcgat
     3661 gctgccgcaa taggcgaggt ggttgtcttt gatgagcacg gcgtcgtcca agcccatgcg
     3721 gtggttcacg ccgccgcctg ccctgacggc gtatttttgc aggacgcgca gcagggggat
     3781 ggttttgcgg ctgcacacga tgtctgtacc gtattcggcg acttcggcaa cggcacgcgc
     3841 ggtggcggtg gcgatgccgc ttaagtgcgt gaggtagttg agcgcggtgc gttcggcggc
     3901 gagcagcgcg cgggcgttgc cttcgacggc agccagcgtc tgacctgcgc ggacgacttg
     3961 cccgtctcgg atttcggctt ggaagcgaac ggacggatcc atcgtctgaa aggcgaggcg
     4021 cgccaagtcc atgccggcga taacgccgtc ttcgcggctg acgaggaaga gtttggcggt
     4081 tttgtcgggg gcgataacgg cggcggacgt aatatcgccg cgcctgccca aatcttcgct
     4141 caaggcttgt tccaccatgg ggcgcaacag ggtgtcgggc agggggaaga gggtgttttc
     4201 agacggcatg gggtttcctt tgttgaaaaa gattgatgtt gcaccgatgg gcaaaacttc
     4261 gtttgatgtc gtctgaaaac gggctaacgg ctgcccagcg gcagtccgat gtccgaaatc
     4321 agcctgtcgg gcaggacgtc gtcgcggaag cggcaaagct gcgcgggacg gcctttgctg
     4381 cccgatacac cggtatccga cggctcgatg aggttttgct gctgaatctg gcggcggaag
     4441 ttttgcttgt gcagcaatct gccgctgatg gcttcgacac tgttttgcag ttgcagcagc
     4501 gtgaattcgg gcggcatcag ttcaaaaatc acggggcggt atttgatttt ggcgcgcagg
     4561 cgagacaggg cggtcgccag tacgcggcgg tggtcgtggc gcatgggctg ccccgtaagc
     4621 gcgaagtcga agtttgcctg cggctcggcg gcctccgcta tcaggccgct ttcatacagc
     4681 atttcatagc gttgcaaaac gtattcttcc gaccagtttt ccggttcgac cccccaacac
     4741 aaatgaatgc gcttgagccg cttttggcgc acttcctccg tgtccgccga gtttgcccaa
     4801 atgcgcaggc ggccgacgac ggcgtcgcgc tgcccgccgt cggtacgcaa atcttcccac
     4861 gggaaatagc cgtagcagtc ctgccatttc gcgtcgggat gcaggatgct gtcggctgcc
     4921 tcgcgcacca gccccaaata gctgacgtac aagacgggca tgccgtgttc gttgcggcgg
     4981 tgggtatcga caaaggtgta aagctgttcc acatagccca taggctgcga agtctgcttg
     5041 gcgacccaca gtttcacgcc cgcctgcagg gaattgcgca ggggggagag cgggccgttg
     5101 ggcagaagcg tgccttgggc gacggtcaat acccgcaggc cgccgtcggt aacggcaatc
     5161 aatacgggaa ccagctcgac gatgctttgc ggcggggctt cggcttcggg gtaggcatcc
     5221 atgctcggct ttctgcgggt gggaaaggct ttattgtata gtggattaaa tttaaaccag
     5281 tacagcgttg cctcgccttg ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc
     5341 tgatttttgt taatccacta taaaccaaag cggtttgtcg gaacgaagcg ggtcggtttt
     5401 acggtatcgc aaacctgttt tcagacgacc ttgcttatta tactcaattt gagcataata
     5461 taaaaatatt tcaaaatata aacggcaaat ctttatgccg tctgaaaaac cagccataag
     5521 gaagtctcat gcaaaccgcc gcccgccgct cgttcgatta cgatatgccc ctcatccaaa
     5581 ccccgacttc cgcctgccaa atccgtcagg cgtgggcgaa ggttgccgac acgcccgacc
     5641 gcgagacggc aggccgtctg aaagacgaaa tcaaggcttt gctgaaggag aaaaacgcgg
     5701 tcttggtggc gcattattac gtcgatccgc tgattcagga tttggcttta gaaacgggcg
     5761 gatgcgtggg cgattcgctg gaaatggcgc gcttcggcgc ggaacacgaa gctagtacat
     5821 tggtggtggc gggtgtgcgc ttcatgggcg agagcgcgaa aatcctctgc cctgaaaaaa
     5881 cagtgctgat gcctgatttg gaggcggaat gttctttgga tttgggctgt ccggaagaag
     5941 cgttttcagc gttttgcgac caacaccccg accgcacggt cgtagtgtac gccaacactt
     6001 ccgccgccgt gaaagcgcgt gccgactggg tggtaacgtc ttcggtggcg ttggaaatcg
     6061 tatcgtattt gaaatcgcgc ggcgagaagc tgatttgggg acccgaccgc cacctcggcg
     6121 actacatccg ccgcgaaacg ggcgcggata tgctgttgtg gcagggttcg tgcatcgtcc
     6181 acaacgaatt caaagggcag gagctggcgg cgttgaagac ggaacacccc gacgcggtgg
     6241 tgctggttca tcccgaatcg ccgcaaagcg tcatcgagct gggcgacgtg gtcggttcga
     6301 ccagcaaact actgaaagcc gccgtatcgc gtcccgaaaa gaaatttata gtggcaaccg
     6361 atctgggcat cctgcacgaa atgcaaaagc aggcgcccga caaagaattc atcgccgcgc
     6421 cgacggcagg caacggcgga agctgcaaaa gctgcgcgtt ctgcccgtgg atggcgatga
     6481 attcgctggg cggcatcaaa tacgccctga caagcggacg caacgaaatc ctgttggaca
     6541 gaaagctggg cgaagccgcc aaactgcctt tgcagcgtat gctcgacttc gcggcagggc
     6601 tgaagaaaaa agatgtgttc aacggcatgg gccccgcctg atttgccgtc catattgccg
     6661 tttcagacaa cctctcaaac aaggacaaca ccatgcaaac ggattgcgac gtattgattg
     6721 ccggaaacgg gctggcggca ctgacgctcg ccctgtcgct gcctgaatcg ttccgcatcg
     6781 tcattttgtg taaaaaccgg ctggacgaca ccgccagccg tcatgcgcaa ggcgggattg
     6841 cggcggcgtg gtcgggagag gacgacatcg aaaaacacgt tgccgatacc ttggaagcgg
     6901 gcgcgggttt gtgcgacgaa gccgccgtcc gcaccatcct gtcgcagggc aaaccggcaa
     6961 tcgaatggct gctggcgcag ggcgtggcgt tcgaccaaaa taataacggc ctgcacctga
     7021 cacgcgaagg cgggcatacc tgccgccgaa tcgcccacgt cgccgattac acgggcgaag
     7081 ccgtcatgca gagcctgatt gcccaaatac gccgccgccc gaacatccgc gtttgcgagc
     7141 ggcagatggc gttggacatc caaaccgaat caggcgcggc gtgcgggctg accgtcctca
     7201 accgccaaac gcaagaaacc taccgcatcc gcgcccgcca taccgtactc gcaggcggtg
     7261 gtttgggaca gatttacgcc gccaccacca cgccgcccga atgcacgggc gacgccatcg
     7321 ccatggcaat acgcgcaggc tgcgcagtcg aaaacctcga atttatccaa ttccacccca
     7381 caggcttggc aaggccgtct gaaaacggcc gcaccttcct gatttccgaa gccgtgcgcg
     7441 gcgaaggcgg catcctgacc aaccaagcgg gcgaacggtt tatgccgcat tacgaccgcc
     7501 gcgccgaact cgcaccgcgc gacatcgttg cccgcgccat tgccgccgaa accgccaaac
     7561 aaacgcaaga cttcgtctcg ctcgacatca gccatcaacc cgcagcgttc gtccgccagc
     7621 attttccgtc catccatcga cactgtttat cccaatgcgg cctggatatc acgcgccaag
     7681 ccatccccgt ccgccccgtg caacactaca cctgcggcgg catccaaacc gaccccagcg
     7741 gcagaacttc cctgccgcag ctctacgcct taggcgaaac cgcctgcaca gggttgcacg
     7801 gagccaaccg cctcgccagc aactccctac tcgaatgcgt cgttaccgcc aggcttgccg
     7861 cccaaaccat cgcggatgga caagcgttcc aaaccgtacc gtccaaaagg tcgtctgaaa
     7921 acacctccgc cgaagcagac atcttttcag acggcatcca aaacacattc agccgcccca
     7981 ccctgcaagc gttcaaccaa cgccatctgg gcatcctccg caacgatacc ggcctgcgcc
     8041 gcgccatcgc ccaactgcgg ctttggaagc aaaaccaaac cgagccgcac actgcgtccg
     8101 aatacgaaaa ccgcaacctg ctcgaatgca gcctcgccgt cgcccaagcc gcatacgcaa
     8161 ggcggcaaaa catcggcgcg cattttaata gtgattgtta aggttggcag gtcagagaaa
     8221 atcgttcaaa taaatttgaa tagcatggtt aagaaattgg cattcggttt ttagggttgc
     8281 gtaagttggg ttatcaatct gttctaaaag cagaaaggcc gagacctttg caaaattccc
     8341 caaaatcccc taaattccca ccaagccatt taggggattt tccatgagca ccttcttccg
     8401 gcaaacc
//
LOCUS       DAWFLT010000079         8368 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWFLT010000079 DAWFLT010000000
VERSION     DAWFLT010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8368
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.079"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(329..604)
                     /locus_tag="WGR74_001751"
     CDS             complement(329..604)
                     /locus_tag="WGR74_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0906120.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(601..1884)
                     /locus_tag="WGR74_001752"
     CDS             complement(601..1884)
                     /locus_tag="WGR74_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0906121.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWPEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWIFGAVQAVTVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(2040..3458)
                     /gene="glnA"
                     /locus_tag="WGR74_001753"
     CDS             complement(2040..3458)
                     /gene="glnA"
                     /locus_tag="WGR74_001753"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ0906122.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDATVVLTCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGIEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDAGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            3542..3691
                     /locus_tag="WGR74_001754"
     CDS             3542..3691
                     /locus_tag="WGR74_001754"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906123.1"
                     /translation="MPSETRPASNFKLENIVSDGKIVNNMLLFVCSKAEYPPFCPNAD
                     KTDAV"
     gene            3769..4578
                     /gene="aroE"
                     /locus_tag="WGR74_001755"
     CDS             3769..4578
                     /gene="aroE"
                     /locus_tag="WGR74_001755"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ0906124.1"
                     /translation="MHALPRYAVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIFLKNGKLRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTHAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSPEIFLGCRLAYDMVYGDA
                     AQEFLNFAQSNGAAEVSDGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAM"
     gene            4580..5281
                     /gene="mtgA"
                     /locus_tag="WGR74_001756"
     CDS             4580..5281
                     /gene="mtgA"
                     /locus_tag="WGR74_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ0906125.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHQTAFMSMR
                     MKQFEQEGRDVALDYRWMPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(5606..5681)
                     /locus_tag="WGR74_001757"
     tRNA            complement(5606..5681)
                     /locus_tag="WGR74_001757"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5646..5648),aa:Phe,seq:gaa)
     gene            complement(5872..6606)
                     /gene="lptB"
                     /locus_tag="WGR74_001758"
     CDS             complement(5872..6606)
                     /gene="lptB"
                     /locus_tag="WGR74_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ0906126.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVTLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRSPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(6680..7210)
                     /gene="lptA"
                     /locus_tag="WGR74_001759"
     CDS             complement(6680..7210)
                     /gene="lptA"
                     /locus_tag="WGR74_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ0906127.1"
                     /translation="MIQKICKLFVLIAFFSVSPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(7191..7772)
                     /gene="lptC"
                     /locus_tag="WGR74_001760"
     CDS             complement(7191..7772)
                     /gene="lptC"
                     /locus_tag="WGR74_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ0906128.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSSKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(7769..8305)
                     /locus_tag="WGR74_001761"
     CDS             complement(7769..8305)
                     /locus_tag="WGR74_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0906129.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
BASE COUNT         1934 a         2267 c         2178 g         1989 t
ORIGIN      
        1 gttgacatat tgtgcgatat cgagggctgt accgtatttt cccatcgtat tttcccgttc
       61 tttatatgag cggcaggcat cctcataaaa gaagggatac cgataaggaa aaataccccg
      121 gcataaaaaa tatactgatt aatcaataca aatccaacaa acagttcatc gtattctttt
      181 ctgttggcgt ataaaaaata ctgatatatg ccttcacttt catagggcga cgctttatgt
      241 accgtcctgt gtgttgaaac gtcggttttc cttaccgcct gccccgcttt tccctgattg
      301 ctgccaactg ccgtccgaac ctgaaccgtc aggtttcaga cggcattttt tctgcctgct
      361 cccacaattg ccgtttttgt gcggcggtca gttttttgac ggcgatttcc tgcctgagtg
      421 ccgttccttt atccatcccg cctgcaacga tacgcatcgc caccggtttg aatacgcggg
      481 tatatttcgc gcctttaccg gttgtgtggg cggcaagccg ctgttgcgga ttcgggctga
      541 tgccgcaata gaacgcgctg ttttcacaca atatcaggta aacgctccag tttgacgcgt
      601 tcatctgcct gcatcctgag ttttctcccc gttccaaggc gcaactttca gcagcagcag
      661 catacccggt agggcgagtg cgaaacacag ctggaaaaac ggtacatacc cgagccattc
      721 gatcagataa ccggcaaagg aattgatgac cgtacgggga acagcggaca ggctggtaaa
      781 cagcgcaagc tgcgttgcgg taaatgcggg attggtttca cgcgccatat acgatacgaa
      841 cgccgccgtc cccaaaccca cgccgactgc ctctgcgccg ataactgccg ccagcatcag
      901 cctctcgcct gtgccgaccg tgtcgaaatg tccgaaccct gccagccata caaaccccaa
      961 aacggttaca gcctgcaccg cgccaaatat ccacaaggct ttgtttacgc cgattttcag
     1021 catccacacg ccgcccaaga tacctgccgc cactgccggc cacagtcctg catttttcgc
     1081 aatcaaaccg atgtctgtct tgctgaaacc catatccaga taaaacggcg ttgccaacgc
     1141 ggttgccata ctgtcgccga gtttgtaaag gaagataaac agcagcacgc acaccgccga
     1201 agcgatgccc ttgcgcgtaa aaaattcttt aaacggctct accacggtct gcttcaacgt
     1261 tttaggaacg gcaggcggca acacgggttc gcgcgcaaga aacagcgtca tcagaaggcc
     1321 gggcagcata aacagcgaag tgatgacaaa cacctccggc cacggcatcc tgtctgccaa
     1381 caccaaactc aatgaaccgg gaatcagggc ggcaatccgg taggcgttca catgaaccga
     1441 gttgcccaaa cccaattctt cgtctgacaa aatctcgcgc ctgaacgcat ccaatacaat
     1501 atcctgactg gcggaaaaaa aagcgacaag caccgacaag ccggcaatca gcggcagatg
     1561 attacggggg tttaaaaagg cataggccgc caaagccgcc agcaaccctg cctgcgtcag
     1621 cagcatccac ccgcgccgcc gtcccaaaac gggcagcctg accgcgtcca tcagcggcga
     1681 ccacaaaaat ttccaagtaa acggcagacc gattaacgcc atcagcccga tgctcttcaa
     1741 atccacctgc tcgctgcgca accacgccgg aatcaggtta atcagaaagt acagcggcag
     1801 ccccgaggta aaccccgtaa aaatacagac aagcatattt cgggaaaaga tttgcccgat
     1861 aaaacctgat ttcgatgcag tcatcgttcg tgcagcccaa aaaaaacaaa agcctattgt
     1921 agcaaatatc gcccgcctgc tgaaaaatgc cgccccgcgc cgccggtttg ccgttcaaat
     1981 gcaaaatgcc gtctgaaact gtgttcagac ggcattgctt ttctgaaaac cagacgtgct
     2041 tacaggctgt aatacatttc aaattccagc gggtgcggcg ccatgcggat gcggcgtacg
     2101 tcttcttctt tgaaagcgat gtagctgtcg atccagtctt tgctgaacac gccgccgcgc
     2161 aggaggaatt cgtggtcggc tttgagggcg gccagtgctt cttccaaaga agcgcaaacg
     2221 gtcggcacca atgcatcttc ttccggcggc agatcgtaca ggtttttatc ggcagggtcg
     2281 cccggatgga ttttgttttg aatgccgtcc aaacccgcca tcaacagggc ggcaaatgcc
     2341 aaatacgggt tggcggtcgg atcggggaaa cgcgcttcga tgcggcgcgc cttgctgctg
     2401 ttcacggacg gaatgcggat ggaagcggaa cggtttttgg cggagtatgc cagtttggtc
     2461 ggcgcttcaa agtgcggcac gaggcgtttg taggagttgg tggacggatt ggtaatcgcg
     2521 ttcaaggctt tggcgtgttt gatgatgccg ccgatgtagt agagcgcggt gtcgctcaag
     2581 ccggcatagc cgtcgcctgc gaacaggttt tgaccgtctt tccaaatgga ttggtgaacg
     2641 tgcataccgc tgccgttgtc gcccataatc ggtttgggca taaaggtggc tgttttgccg
     2701 aagttgtggg caacgttttg aatcacatat ttcatgtctt gggtttggtc ggcgcgtttg
     2761 accaaggtgg cgaaacgcgt gccgatttcc atttggctgc cggtaccgac ttcactgtgg
     2821 tggacttcga cttcgatgcc gagttcttcc aaaatgttta ccatcgcgga gcgcaggtct
     2881 tgtcccgcgt caatcggagc aacgggcgcg taaccgcctt tgacggcagg gcggtggccg
     2941 gtgttttgac cgtccatatg cagaccgctt gcccacgcgc cgctttcgga cgtgatttcg
     3001 taacgggttt tgtgcatatc ggtttcaaat tctatgccgt cgaatacgaa aaactcgggt
     3061 tcgggaccga aataggcggt gtcgccgatg ccggaagatt tcaaataggc ttcggctcgg
     3121 cgggcgatgg agcgcgggtc gcggtcgtaa ccctgaccgt cggcgggatc gataacgtcg
     3181 caagtcaaca caacagtcgc atcatcataa aaaggatcga cgaaggctgt agacgcatcg
     3241 gggcgcaact gcatatcgga agcctgaatg cctttccagc cgccgataga cgaaccgtca
     3301 aacgcctgac cgttttcaaa ccactcttcg gggtcttcca acacgatgcg cgcaggcacg
     3361 gtaaagtggt gctgcttgcc tttggtatcg gtaaagcgca aatcgacaaa gcgggcttcg
     3421 ctttcttcaa tcaattttac ggcgttttta atagacattt ttcagctcct ggaaaaggga
     3481 aatagcaaaa tatgcggaac gcggcggctt gacgcaaagt cggcatgatt ctaccacgtc
     3541 catgccgtct gaaactcgcc ctgcgtcaaa tttcaagctg gaaaatatag tttcagacgg
     3601 caagattgtc aacaatatgc ttctgtttgt ctgttcaaaa gcggaatacc cgcccttttg
     3661 tccgaatgcc gacaaaacag atgccgtctg aaagcagttt agacggcatc gttcccatct
     3721 tcgcgccgcc gttataaaat gaccgccccg ccttgatgga atatgactat gcacgccctc
     3781 ccccgctacg ccgtttttgg caaccccgtc gcccacagca agtcgccgca gattcatcaa
     3841 caatttgccc ttcaggaagg cgttgacatt gaatacgaac gcatttgcgc cgacatcggc
     3901 ggtttcgcgc aggcggtttc gacatttttt gaaacaggcg gttgcggggc aaacgttacc
     3961 gtaccgttca agcaggaagc gtttcatctg gcggacgagc attctgaacg cgcattggct
     4021 gccggcgcgg tcaacacgct gatttttctg aaaaacggaa aactgcgcgg cgacaatacc
     4081 gacggtatcg gtttggccaa cgacatcacg caggtcaaaa acattgccat cgaaggcaaa
     4141 accatcttgc ttttgggcgc gggcggcgcg gtgcgcggcg tgattcctgt tttgaaagaa
     4201 caccgtcctg cccgtatcgt cattgccaac cgcacccacg ccaaagccga agaattggcg
     4261 cggcttttcg gcattgaagc cgtcccgatg gcggatgtga acggcggttt tgatatcatc
     4321 atcaacggca cgtccggcgg cttgagcggt cagcttcctg ccgtcagtcc tgaaattttc
     4381 ctcggctgcc gccttgccta cgatatggtt tacggcgacg cggcgcagga gtttttgaac
     4441 tttgcccaaa gcaacggtgc ggccgaagtt tcagacggac tgggtatgct ggtcggtcaa
     4501 gcggcggctt cctacgccct ctggcgcgga tttacgcccg atatccgccc tgttatcgaa
     4561 tacatgaaag ccatgtaata tgttccgcat cgtcaaatgg ctgattgccc tgcccgtcgg
     4621 catctttatc tttttcaatg cctatgtgta cggcaacatc attacctacc gcgccgtcgc
     4681 gccccatcag actgccttta tgtcgatgcg gatgaagcag tttgaacagg aaggtcgcga
     4741 tgtcgcactg gattaccgct ggatgcccta caaccgcatc tcggtcaacc tgaaaaaagc
     4801 cctgattgct tccgaagatg cccgtttcgc cgggcacggc ggcttcgatt ggggcggcat
     4861 tcaaaacgcc atcaggcgca accggaacag cggcaaagtg aaggcgggcg gctcgaccat
     4921 cagccagcag cttgccaaaa acctcttcct caacgaaagc cgcagctata tccgcaaagg
     4981 cgaagaagcg gcgattaccg cgatgatgga agccgttacc gacaaagaca ggatttttga
     5041 actgtattta aactcaatcg aatggcacta cggcgttttc ggcgcggaag ccgcgtcccg
     5101 gtatttttat caaatacccg ccgccaagct gaccaaacag caggcggcaa aactgacggc
     5161 gcgcgtcccc gccccgctct actacgccga ccatccgaaa agcaaacggc tccgcaacaa
     5221 aaccaatatc gtgctcagac gcatgggttc ggcagagttg cctgaaagcg acacggactg
     5281 attgtttcag atatggaaat gccgtctgaa ctgcggttcg gacggcatat gttttctggt
     5341 ttttatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtacaaatag
     5401 cacggaatcg attcacttgg tgcttcagca ccttagagaa tcgttctctt tgagctaagg
     5461 cgagccaacg ctgtaccggt ttaaatttaa tccactatac ctgcccgaca gccgcaaaga
     5521 ccgtatcata catagcaggc attgcggtgc gggattactg ccgcaaacgg caggcaaaaa
     5581 aaataaccgc ttcaaggcgg ttttttggtg gccagaggcg gaatcgaacc gccgacacac
     5641 ggattttcaa tccgttgctc taccaactga gctatctagc ctttgtctgt ctcgttaaga
     5701 gatgcgtatt aaaccaaatt acgggacgac atgcaagcat atcgggcaat attctttgaa
     5761 aacaggttta agtcaatgtt ttaaatttga attaatttat gtaaaaacaa cataaatccg
     5821 tatttttgcc gcctgcccga cgatattagg tcgcccgtct gaaaaatatt ttcaatattt
     5881 gaagttctta cccagataaa cagaacgaac ctgttcgttt ccgaccaaat catcaggttt
     5941 tcccgatgcc agcaccgcac cgtctgaaat gatgtaggcg cggtcgcaga tgctgagggt
     6001 ttcgcgtacg ttgtggtcgg taatcagtac gccgataccg cgcgacttga gaaatccgat
     6061 gattttttgg atgtcgatga cggcgatggg atcaacaccg gcaaaaggct catccagcaa
     6121 aataaaatgc ggtttcatgg cgagtacgcg ggcgatttcg acgcgccgcc gttcgccgcc
     6181 ggacagcgac ggcgcggggc tgcggcgcaa gtgtccgata ttaaggtcgg cgagcagttt
     6241 ttcgatttcc ctgtcgattt gatttttatc tttggttctg atttccaaga tggcgcggat
     6301 gttttgttcg acggtcattt tgcggaatat cgaggcttcc tgcggcaggt agccgacacc
     6361 gaggcgggcg cgttcgtgta tgggcaggtg gcgcaattct tgtccgtcta gggttacgct
     6421 gcctgcgtcg gcggcaatga gtccgactat catgtagaag ctggtggttt tacccgcgcc
     6481 gttgggcccg agcaggccga cgacttctcc gctttcgatt tcgagggaga agcttttaac
     6541 gacttggcgt tttttgaaac ttttttgcag gttttgaaca acaaggcggc tgacgtttgc
     6601 gctcataaat atctcttcaa tatcttcggt cggcaaatgc cgtccgaacc aggtttatgt
     6661 ttcagacggc attttgggat tattcggatt tttgcgtact cgaaggctgg ataacgacgc
     6721 tgaccctgcc ggatttggaa gcggattttg cgccggattt tgtgctgccg ctgatggtat
     6781 agacttcggt tttggtgttg tatgtaatca ccgcaccttc ggcgacatcg ccgccgcgct
     6841 gtactttggc attaccggtt aagactacgg tgctgcctgc agatgaataa gcaacgttgt
     6901 ttgcctgtcc gcgcaccgtg cctttgccgc cgtccaatgt ctggctgaag cggactggcg
     6961 aaccttccgc cctcacggat tcgccgcctt tgccgccgcg tgtaacattg acgcgggcgg
     7021 cggaaatatt gagcgtaccc tgtctgatga cgacgtttcc gctgaatgtg gtgctttggt
     7081 tggcttgatc gagcgaacct tggtcggcct caatctgaat aggctgcctg ctgtcgcttt
     7141 gaagggcaaa agcgggggac accgaaaaaa atgctattaa aacaaatagc ttacatatct
     7201 tttgtatcat aaatcgtggc tttcacttta gatgagaagt tcaacatgcc tgttttgtgg
     7261 tcgtaagtca tgccgcccgc ctgaccgtgc gatgcaccat attggaaact gacaggcgta
     7321 tcggtttggg catattgaga ttcggtatcg acgtgcagct tttcggcttc aactttaccc
     7381 gcctgccgtt tgccgtcggc ggttttggtc agcacaacgt tgtttttaaa aagaacctgt
     7441 ttgttttcgg tatggtaaac ggcttcatcg ctgccgactt cgtacaacaa cctgccttct
     7501 tggaagaaca cgagatgcgg tgagtcgaaa tgaatgtcgc tgctttcggg aaactgtttc
     7561 gcgcccttcg aactcaaatg ttctttcaag tatccctgtt cgtcaaaccg cctgccatcc
     7621 aatccgtcca ttgtgtattg cggttcgtcg ggattgagcc tgacttcttc aatctcgact
     7681 tcgctgatgc gtcccaacca tgccgacagg ctgcccaagg caaccgccaa tatcaatggg
     7741 aacgcaattc cgtaccgcca tcttactttc atttgatgta ctcgttcaaa gccgcgccca
     7801 aagtcccttg cgcctgcata atcaggtcgc acacttcgcg caccgcgccc gcaccgccgg
     7861 ggcgtttggc aatataggcg gcgtgttgca gcgtaaacca atgcgcgtcg gggacggcaa
     7921 ccggcaatcc gcagcgtacc ataacgggca aatcgaccac gtcgtcgccg acaaaggcac
     7981 actcggcttc ttccacgccg acctgagcgc gcagctcttc atacgcggcg cgtttgtccg
     8041 aaatgccttt gaagtagtaa tttatgccca actgtttgac gcggatgccg acggagggcg
     8101 cgtcccgacc tgtgataatc gcagtctgca cgccgcttgc ctgaagcatt ttcagaccgt
     8161 gtccgtccag tgtgtgaaac gatttgattt cttcgccgtt atcgcggata aagatgcgcc
     8221 cgtcggtcaa aacgccgtcc acatccagga tcagcagttt gattttggcg gcgcgcgcct
     8281 gtaattcggg agaaattgcc tgcatgggat ttccttgttt tcagacggca tatcttacca
     8341 caaatgccgt ctgaaacggc tgcaacgc
//
LOCUS       DAWFLT010000080         8343 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWFLT010000080 DAWFLT010000000
VERSION     DAWFLT010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.080"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(116..1219)
                     /locus_tag="WGR74_001762"
     CDS             complement(116..1219)
                     /locus_tag="WGR74_001762"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ0906130.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(1454..3010)
                     /gene="dnaA"
                     /locus_tag="WGR74_001763"
     CDS             complement(1454..3010)
                     /gene="dnaA"
                     /locus_tag="WGR74_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ0906131.1"
                     /translation="MTLAEFWPLCLRRLHDMLPHGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPNEPRQAAGSASRPELEV
                     LAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VSASSRFMNRPVIDMDLARTALQDIIAEKHKVITADLIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            3303..3437
                     /gene="rpmH"
                     /locus_tag="WGR74_001764"
     CDS             3303..3437
                     /gene="rpmH"
                     /locus_tag="WGR74_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ0906132.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            3440..3805
                     /locus_tag="WGR74_001765"
     CDS             3440..3805
                     /locus_tag="WGR74_001765"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ0906133.1"
                     /translation="MDYRFGKQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLGH
                     PRIGLVIGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVHRKFDRPTAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            3870..4091
                     /gene="yidD"
                     /locus_tag="WGR74_001766"
     CDS             3870..4091
                     /gene="yidD"
                     /locus_tag="WGR74_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ0906134.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            4263..5900
                     /gene="yidC"
                     /locus_tag="WGR74_001767"
     CDS             4263..5900
                     /gene="yidC"
                     /locus_tag="WGR74_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ0906135.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGECSIDI
                     KRRNDKLYSASVSVPLAAIPAGAKAETAVNLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRTQGEVVS"
     gene            complement(5981..6694)
                     /locus_tag="WGR74_001768"
     CDS             complement(5981..6694)
                     /locus_tag="WGR74_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ0906136.1"
                     /translation="MVSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAEKTAR
                     AHLKHLGVTMPIRELNLQTLNEHTDLKTLLELLKPLQEGRSMGIVSEAGCPAVADPGA
                     NLVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQSLKALEQ
                     RSRQQNETQLFIETPYRNDVLLADAVENLHSETRLCVAADLTLPTQEIISRTIAQWRK
                     RKEMPNLKKRPTIFVMYAG"
     gene            complement(6753..7343)
                     /gene="maf"
                     /locus_tag="WGR74_001769"
     CDS             complement(6753..7343)
                     /gene="maf"
                     /locus_tag="WGR74_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ0906137.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPEALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEGL
                     GALLIERIESSDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            7491..7994
                     /locus_tag="WGR74_001770"
     CDS             7491..7994
                     /locus_tag="WGR74_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ0906138.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            8028..8207
                     /gene="rpmF"
                     /locus_tag="WGR74_001771"
     CDS             8028..8207
                     /gene="rpmF"
                     /locus_tag="WGR74_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ0906139.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
BASE COUNT         2057 a         2482 c         1989 g         1815 t
ORIGIN      
        1 gccgtactgg tttttgttaa tccactatac ctttccgaca gccgaacaaa accccgaatc
       61 cgtctgcacg gttcggggca tatctccaat acgggcatcg tgctccggaa aaccgtcaaa
      121 ttcgcatcgg catcacgata tatttgaaat tcggattgtt cggcacggta aacagcgtcg
      181 agcggttggc atcgccgaag gcaagctgca tatcgtcgga atggatgttg cgcaacacgt
      241 ccatcagata gccgatattg aaaccgactt cgagttcgcc gccctgatag gcaatctcga
      301 tttcttcgcg cgcttcttcc tgctcgttgt tgctgcacac gacgctcaac aggccggatt
      361 gcaggaacag gcgcgcgcca cggaattttt cgttggcaag aatggacaca cgttccagcg
      421 cgcccaaaag ttcggcacgg gacaaaacga aaatcttgtc gttgtccaaa ggaatcacgc
      481 ggttgaaatc ggggaatttg ccgtcgatga ccttgctgac gatggtcgtg ccgttgcatt
      541 ggaaacgcac ctgtttgtcc agcagctcga tttgaatcgg atcgtcgggg ttgttcaaca
      601 gtttgaacag ttccagcacc gttttgcgcg gcaaaatcac ttcggcgcgc ggcaaatccg
      661 catcaatcgc gcaggctgca taggcaaggc ggtgtccgtc ggtcgccaca aggcgcaact
      721 ggctgccctc aacctgcatc agcagaccgt tgagataata gcggatgtcc tgcaccgcca
      781 tgctgtactg cacttgcgac agcatggttt tgaaacgctc ctgctccagc gagaaagtcg
      841 cgctgatgtc ctcgccgaca ttcatcatcg gaaaatcggc ggcaggcagg gtctgcaggg
      901 caaaacgcga tttgcccgcc ttcagcgtca gacggctgtc gtcccaatcc agcgacacca
      961 gcgcaccggc aggcagtgcc cgtaaaatgt cctgaaattt cttggcattg gtggtgatgc
     1021 ggaagtcgtc cgcgccgccc tcgggacccg cagtgtcgat ttggatttcc aaatcggttg
     1081 ccaagagttt ggtctgaccg ccttttccct caatcaggac gttggacagg atgggcaggg
     1141 tgtggcggcg ttcgacgatg ccggtaacgg cttgcagcgg cttgagtagg ctgtcgcgtt
     1201 cggcttgtaa aatcaacatt ttcgctcctt tgaatcggtt tgtatagtgg attaaattta
     1261 aatcaggaca aggcgacgaa gccgcagaca gtacaaatag tacgaaaccg attcacttgg
     1321 tacttcagca ccttagagaa tcgttctctg tgagctaagg cgaggcaacg ctgtactggt
     1381 ttaaatttaa tccgctatat ctttaccctt cggacggcat gggcaatatc atgtcgtctg
     1441 aaaacgtttt ccatcagttt tgaatcagaa tcagcagttt ctcgtaatcc tgcgccaact
     1501 cggggtcttc ctcgcgcagt ttcgccaccg ccctgatgcc gtgcatgacg gtcgtatggt
     1561 cgcgtccgcc gaacgaatcg ccgatagacg gcaggctcaa agtggtcagt tctttggtca
     1621 ggctcatggc aacctgacgc ggacgggcaa tgttgcgcgt gcgttttttg ccgagtacgt
     1681 cgctgatttt gatgcggtaa tatttcgcca ccgcatcaat gatgaggtcg gcggtaatga
     1741 ctttgtgctt ctcggcgata atgtcctgca acgccgtacg cgccaaatcc atatcgatga
     1801 cggggcggtt cataaagcgg ctgctcgcgc tgacgcggtt gaatgcgcct tcaagctcgc
     1861 gcacattgga acggatcagg ttggcaataa acaatgccgc ctcgtcttcg atgctgatac
     1921 ccgccgcctc tgcctttttc tgcaaaatgg cgatgcgcat ttccaattcg ggcggctcga
     1981 gttccaaagt caaaccccaa gagaaacgcg atttgagccg gtcgtccatg ccttcgattt
     2041 tggcgggtaa aacatcgcaa gtgaggatga gctgtttttt ctcgttgtgg aaatggttgt
     2101 acagatagaa aaactcttcc atcgtacggt ctttgccttt gatgaactgg atatcgtcga
     2161 taatcagcag gtcgtattgc ttgtattgct gcttgaacac gtcgtaggta ttgttgcgaa
     2221 ccgccttcat aaagctgcgg atgtagtcgt ccgaatgcat atagcgcact ttcgcatcgg
     2281 gacggttttt caaaagctcg ttgccgaccg cctgcacaag gtgggttttg cccaaacccg
     2341 tgctgccata gaggaagaac gggttataac tctgtcccgg actttccgct atcgcctgcg
     2401 cggcggcagc tgcaaggcgg ttgcccttac cttcgaccaa cgtatcaaac gtgtaatccg
     2461 gagacaggtt ggtttgttcg taacgcgcct cttccgcatc acgctgcgca tccgtccgcg
     2521 ctttcgcaag cacctccaat tccggacggg aagcagaccc ggcagcctga cgcggctcgt
     2581 tcggcaggtt tttcatacgt tccgccaaaa tatccgccgc cgttttcgac gcagcgggtt
     2641 tgacaggctc ttcagacggc agctcgtcca acagaacctc ctgcacaggc atctcctccg
     2701 accccgcgtg caaggacggc tcggcaggtt cgacagcacc ttcaaccgcc gccatctcat
     2761 aacgcacgcc ttctcccggt ttgaatacga aggcggaacg gccggcagcc aactcttccc
     2821 tcaccgcttc tatttttccg gcaaactggc tcttgagcat attgcaggca aactggttct
     2881 tgccgtacac cacccatacg ccaccctcct caccgacggt aaggggcgca atccattgcg
     2941 caaactgccc gtgaggcaac atatcgtgaa gacggcggag gcacagcggc caaaactctg
     3001 ctaatgtcat ggataggctc gaatcggtaa aaatgaaatc gaaaacaaag aaaatataat
     3061 attttcaaaa agaaaacaaa tctgttgaac acacatcggt tcaaaatgca gcgaaccgat
     3121 tataccgact cgccgatatt ttatccacaa cccgtgcaaa aatttatcca cagaaaggcg
     3181 gcagaaatcc gcaggcaatc gggcaatcct cctgcaaagt ttctatattg attgacaaaa
     3241 gcggcaaatt ggagtgtaat tcacggttta attatctacc cattctattt ttaggaaaca
     3301 tcatgaaacg cacttatcaa ccttccgtta ccaaacgcaa acgcacccac ggcttcctgg
     3361 tgcgctccaa aacacgcggc ggccgcgcag tactggcagc acgccgtgcc aaaggccgca
     3421 aacgcctggc agtgtaattt tggactaccg cttcggaaag cagtaccgct tgttaaaaac
     3481 ggatgatttt tcatccgttt ttgcattcag aaaccgccgc agccgcgacc tgctgcaagt
     3541 ttcgcgctca aacggcaacg ggctgggcca tccccgcatc ggtctggtga tcggcaaaaa
     3601 aaccgccaaa cgcgccaacg aacgaaatta tatgaagcgc gtcatccgcg actggttcag
     3661 attgaacaaa aaccggctgc cgccgcagga tttcgtcgtg cgcgtccacc gtaaattcga
     3721 caggcctacc gcaaaacagg caagggcgga actggcacaa ctcatgttcg gcaaccccgc
     3781 aaccggatgc aggaaacagg catgatcaga acggtactct gcaggcaagg ttcagacggc
     3841 aacgggtttc ccatacaagg aacatcccga tgaacttcct attgtccaaa ctcctgctgg
     3901 gactgatacg gttctaccaa tattgcatca gcccgctgat tccgccgcgc tgccgttata
     3961 cgccgacctg ttcgcaatac gcggtcgaag cggtcaaaaa atacggcgca ttcaaaggcg
     4021 gtcggctcgc catcaagcgc attgcacgct gccacccttt cggcggacac ggacacgacc
     4081 ccgttccctg acccgacgca atattcaaat tgcacgcttt ccttttattt cccatcggtt
     4141 tctatataat gccgtctgaa acttcgggct ggcggcacga ccgtcgggta tgaagcccac
     4201 ccttattccc cgtctatcgg aacacgcaac ctgcggcatt tccgaccatt caggaaactc
     4261 ttatggattt taaaagactc acggcgtttt tcgccatcgc actggtgatt atgatcggat
     4321 gggaaaagat gttccccact ccgaagcccg tccccgcgcc ccaacaggca gcacaacaac
     4381 aggccgtaac cgcttccgcc gaagccgcgc tcgcgcccgc aacgccgatt accgtaacga
     4441 ccgacacggt tcaagccgtc attgatgaaa aaagcggcga cctgcgccgg ctgaccctgc
     4501 tcaaatacaa agcaaccggc gacgaaaata aaccgttcat cctgtttggc gacggcaaag
     4561 aatacaccta cgtcgcccaa tccgaacttt tggacgcgca gggcaacaac attctaaaag
     4621 gcatcggctt tagcgcaccg aaaaaacagt acagcttgga aggcgacaaa gttgaagtcc
     4681 gcctgagcgc gcctgaaaca cgcggtctga aaatcgacaa agtttatact ttcaccaaag
     4741 gcagctatct ggtcaacgtc cgcttcgaca tcgccaacgg cagcggtcaa accgccaacc
     4801 tgagcgcgga ctaccgcatc gtccgcgacc acagcgaacc cgagggtcaa ggttacttta
     4861 cccactctta cgtcggccct gttgtttata cccctgaagg caacttccaa aaagtcagct
     4921 tctccgactt ggacgacgat gccaaatccg gcaaatccga ggccgaatac atccgcaaaa
     4981 ccccgaccgg ctggctcggc atgattgaac accacttcat gtccacctgg atcctccaac
     5041 ccaaaggcgg acaaagcgtt tgcgccgctg gcgaatgcag catcgacatc aaacgccgca
     5101 acgacaagct gtacagcgca agcgtcagcg ttccccttgc cgccatcccg gccggcgcga
     5161 aagccgaaac cgccgtcaac ctgtatgccg gcccgcaaac cacttccgtc atcgcaaaca
     5221 tcgccgacaa cctgcaactg gccaaagact acggcaaagt acactggttc gcctcccccc
     5281 tcttttggct tttgaaccaa ctgcacaaca tcatcggaaa ctggggctgg gcaatcgtcg
     5341 ttttgaccat catcgtcaaa gccgtcctct acccgctgac caacgcgtcc taccgctcga
     5401 tggcaaaaat gcgcgccgcc gcacccaaac tgcaagccat caaagagaaa tacggcgacg
     5461 accgtatggc gcaacaacaa gcgatgatgc agctttacac agacgagaaa atcaacccgc
     5521 tgggcggctg cctgcctatg ctgttgcaaa tccccgtctt catcggattg tattgggcat
     5581 tgttcgcctc cgtagaattg cgccaggcac cttggctggg ttggattacc gacctcagcc
     5641 gcgccgaccc ctactacatc ctgcccatca ttatggcggc aacgatgttc gcccaaacct
     5701 atctgaaccc gccgccgacc gacccgatgc aggcgaaaat gatgaaaatc atgccgttgg
     5761 ttttctccgt catgttcttc ttcttccctg ccggtctggt attgtactgg gtagtcaaca
     5821 acctcctgac catcgcccag caatggcaca tcaaccgcag catcgaaaaa caacgcaccc
     5881 aaggcgaagt cgtttcctaa atgccgcagc atgaaaaatg ccgtctgaaa cctgttcaga
     5941 cggcattttt attgttcacc ccctatcggg gcggaaatct tcaacctgca tacatcacaa
     6001 aaatcgtcgg gcgttttttc agattgggca tttctttcct tttccgccac tgcgcgattg
     6061 tccggctgat gatttcctgt gtcggcaagg tcaaatccgc agctacacac aagcgcgttt
     6121 cagaatgcag gttttccacc gcatccgcca acaacacatc gttgcgatac ggcgtttcga
     6181 taaacagctg cgtttcattc tgctggcgcg aacgttgctc caaagccttc aaactctgaa
     6241 tccgctcgtt tttttcagac ggcagataac ccttaaacgc aaaactctgc ccgttcgcac
     6301 ccgaagccat caaagccagc agcaggctgg aaggcccgac cagcggacgc acttcaaaac
     6361 cgtgtttatg cgccaaagcc accagattcg cacccggatc ggcaacagcc gggcaacccg
     6421 cctcgctgac aatgcccata ctgcgccctt cttgcaaagg tttcagcaat tccagcaaag
     6481 tcttcaaatc cgtgtgttcg ttcagcgttt gcaaattcag ttcgcggata ggcattgtca
     6541 cgcccaaatg tttcaaatgc gcgcgcgccg ttttttccgc ttccacgaca aaatctgtca
     6601 gcccgacaat cgcctgttgt tcatgcggca acaggcacgg cgtgtcaggc gtacccaaag
     6661 gcgtaggaat caaatacaaa acaggagaca ccattccctc actcatcagt taaaaatgcc
     6721 gtctgagcct ttcagacggc ataaacgggc agttacagaa cctccacgcc ctcatttttc
     6781 aagaaatcga ccagacggaa aaccggcaaa ccgattaaag cattcggatc gctgctttca
     6841 atccgttcaa tcagcagcgc gcccaagccc tcgctcttca acgcacacga gcaataaacc
     6901 gcatcaggct cgcgctccaa atagcggagg atgtgcagct cgtccaactt cctcatcacg
     6961 accaccgtct tatcgatatg ccgttgcatt ctgcccgtaa ccgtatttag cagaacgacc
     7021 gcgctgtaaa actcaatctc cctgccgctc aaatgcatca acatcttttg cgcattagca
     7081 aggttcatcg gcttgcccca ctgcctgccg tcgcaccacg ccacctggtc cgcaccgaca
     7141 atcaacgcct ctgggaaacg cccggtcaac gaccgcgcct taccctcggc aaggcgcaat
     7201 gccgtctgag gggcggattc ccccaacatc ggcgtttcgt caaaatcggg ggacgccgcc
     7261 tgaaaggcaa tgccgagcct ttccatctgt tcgcggcgga aaaccgaact cgtacccaaa
     7321 atcaaaggca gttccaaacc catcccatcc tccttaccgt tgaaaacacg cccgaagggg
     7381 cagtaaaatc cagccatgcg ccgaaacacg gatacccgcc ttcggcgtac cgcaacattt
     7441 ttcttaaaaa tattgacgtt agaacatcta aattatatca tatcccgttt atgtcagacc
     7501 ctaatttgat tgacccggaa atttttgccg ccgaagggca gaacctgcaa ggcagttttc
     7561 tgctggaaga attggatgaa cgcgtcagtt cgcacgatta tcccgccgac aggcagacca
     7621 aaatatcgtt tacactgacc ggcggtcgcg accggctgca acgcctgttc ctcgacctga
     7681 acgtcaaagc cgatatgccc ctgatttgcc agagatgtat caaacccatg ccgttcatgc
     7741 ttgatgaaag cagccgtatc gtcctgtttt ccaacgaaga gtccttggac gaatccatgc
     7801 ttgccgacga agaactcgaa ggcatactga ttgaaaaaga actcgacgtg cgcacattgg
     7861 tagaagacca aatcctgatg tccctgccct tttcgccgcg acacgaagac tgcggcgaca
     7921 atgggacact ggaagaagtc aatcgggaca aacccaaccc ctttgctgtt ttggcaggtt
     7981 tgaaaagcaa ttgattagga cacagtttat ttatctagga gcttgaaatg gccgttcaac
     8041 aaaacaaaaa atccccttcc aaacgcggta tgcaccgttc gcacgacgcg ctgaccgcgc
     8101 ctgcactgtc tgtcgacagc acaaccggcg aagtacaccg cccgcaccac atctccccca
     8161 acggtatgta ccgcggccgc aaagtggtca aagccaaagg cgaataatcc ctattcgact
     8221 gactgaaaaa gccagaacat tgccatgcaa ttactggctt tttttgcatt ggacgcacca
     8281 tccgtccaaa ctttcgccat acgtcaacac acaggggcaa agcgttccgt ataataaccc
     8341 gtg
//
LOCUS       DAWFLT010000081         8283 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWFLT010000081 DAWFLT010000000
VERSION     DAWFLT010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8283
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.081"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(208..1593)
                     /gene="pntB"
                     /locus_tag="WGR74_001772"
     CDS             complement(208..1593)
                     /gene="pntB"
                     /locus_tag="WGR74_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ0906140.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGINGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TLSSGSQEIGEYREVKAADIAEMLKGANNVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1659..2042)
                     /locus_tag="WGR74_001773"
     CDS             complement(1659..2042)
                     /locus_tag="WGR74_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ0906141.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLARTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(2046..3587)
                     /locus_tag="WGR74_001774"
     CDS             complement(2046..3587)
                     /locus_tag="WGR74_001774"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ0906142.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVSLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQISQGNGFVTLLAFIAILIASINIFGGFFVTRRMLN
                     MFRKG"
     gene            3877..4710
                     /gene="serB"
                     /locus_tag="WGR74_001775"
     CDS             3877..4710
                     /gene="serB"
                     /locus_tag="WGR74_001775"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ0906143.1"
                     /translation="MPHALVLQFPSAEALPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEADAALLGSRQIDHAVLPDMDFDELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGIGVAYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            4774..5997
                     /locus_tag="WGR74_001776"
     CDS             4774..5997
                     /locus_tag="WGR74_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ0906144.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGTDGVS
                     ISFREGVAQASGMRRVAVLTLCGAVAGSGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEGEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     STSLLWFSAVIGPILGVTAVFFQRTAQKFPFIKRDNIKIILLAVCMFALVGAVAVWFP
                     EVLGNGKAGNQLTFGGLTDWQHSLELTAVKWLVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSESAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            6158..7738
                     /gene="purH"
                     /locus_tag="WGR74_001777"
     CDS             6158..7738
                     /gene="purH"
                     /locus_tag="WGR74_001777"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ0906145.1"
                     /translation="MSVIKRALISLSDKTGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLPEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGEPEIGEFPSRFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIATAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHR
                     LNRADLKVVSKRQPTEQEWNDLMFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTDVRHFRH"
BASE COUNT         1837 a         2255 c         2356 g         1835 t
ORIGIN      
        1 taaaaatcca tcttcaacac ggagatggat tttgtttgtt tcgttgattt tgtgtcagtt
       61 tcagatgtag gtgcttattc ggacgggccg tctgaaaatg tttgccccaa tgcaaaaaaa
      121 atcactgcaa accttcataa acggggtttg cagtgatttt ttcaaatcaa acagattgaa
      181 aacctgcgcc gaattgttca gacggcatta ttttttcagt tcggacagaa tgtcatccac
      241 ggttttcttc gcatctccga aacacatcac gctgttttcg ttgaagaaca gtgggttttg
      301 tacacctgcg tagccggtat tcatcgagcg tttgaagacg acgacttctt ttgccttcca
      361 cacttccaac acgggcatac ccgcaatcgg gctgttcggg tcggtttggg cggcggggtt
      421 gacggtgtcg ttcgcaccga tgaccaagac cacatcggtt tcggggaagt cgtcgttgat
      481 ttcgtccatt tccaaaacga tgtcgtaggg gactttggct tcggcgagca gtacgttcat
      541 atgaccgggc aggcggccgg cgacggggtg gatgccgaag cgtacttcgg tgccgttttt
      601 acgtaaaagc tcggtgattt cggcaacggg gtattgcgct tgtgcgactg ccataccgta
      661 gcccggggta atgatgacat tgtttgcgcc tttcagcatt tcggcaatat cggcagcttt
      721 gacttctcgg tattccccta tctcttggct gccggaagat aatgtgccgc tgtcgctgcc
      781 gaaaccgccg gcaattaccg agacaaacga gcggttcatg gctttgcaca taatgtagga
      841 cagaatcgcg ccgcttgagc cgaccagcgc gccggtaacg atgagcaggt cgttggagag
      901 catgaagcct gccgctgcgg ccgcccagcc ggagtaggag ttgagcatgg acacgaccac
      961 gggcatatct gcgccgccga tggaggcaac caagtgccag ccgaatgcga gggcaatcag
     1021 ggtcataatc agcaggatga agccgctacc gttaatgccg acgaatacga gcagcaacac
     1081 aaacgatacg gcgagcgcca gtgcgttgag cttgtgtttg gcgggcagtt gcagcgggct
     1141 gctgctgatt ttgccgttga gtttgccgaa tgcgaccagc gagccggtaa aggttaccgc
     1201 gccgatgaaa atgcccaaat agacttcgac cagatggatg gtgtgcatat cgtgcgaaac
     1261 gttgcccggc tcgatatagc tgttgaagcc gaccaaaacc gccgctaggc cgacgaagct
     1321 gtgcagcagg gcaatcagtt cgggcatttc ggtcatttcc acctttttgg ctttgtagat
     1381 gccgattgcc gcgccgatga gcatggcgat gatgatccag cccagtccgt gggtattgtc
     1441 ggaaaaaaca gttacaaaaa gggcgaccgc cataccggcg ataccggaat agcagccctg
     1501 tttggcggtt tcctgtttgg acagccccgc cagtgagaag atgaataaaa ttgcggcaac
     1561 gatatacgcc gctgttacga gtcctgaaga catggaaatt ctccgatttt cgatgatttg
     1621 tttttaatgc cgtctgaact gttcagacgg catctgtttc aagcagccgc gacaaacagc
     1681 ccgacggtgc aggcaaatcc gcccgcccac atcagcgagc gcaatgctgc tttgtctgcg
     1741 atgtagcacc agataaaggc gaggcggaac aggatgaaca ggccggcaag cgtgttgacg
     1801 gttgcttgtc cggcattgcc ggttgcgtgt gccgtcaaaa cggcggcggc aaacggtgca
     1861 aaggcttcaa aaccgttttg ctgcgcggcg tgggcacggg cggctgtgcc ttgcgtgcgc
     1921 gccagaaaat cgcgcggatt gtggttgtct ttaaaccgga atccgcccgc ttttttggca
     1981 tatgccgcac aaaaaagcgg caataagcag gcaatcagga tgcaccaata ggcaaaagtc
     2041 atgctttatc ctttcctaaa catattcaac atgcggcgcg ttacgaagaa gccgccgaag
     2101 atattgatgc tggcaatcag gatggcgatg aaggcaagca gggtaacgaa accgttgcct
     2161 tggctgattt gcagcagcgc gccgacgacg atgatgccgg agatggcgtt ggttaccgac
     2221 atcagcggtg tgtgcagcga gtggctgacg ttccaaacga catagtagcc gatgacgcag
     2281 gcaaggacga acacgataaa gtggttcagg aatgctgcgg gtgcgaccgc gccgacccac
     2341 agcaccaata cggcggcgat gacggcgggc gcgagttttt tccacaggga aacaggtttc
     2401 ggctcgggct tggcggcagg cgcggctttt tcagacggcg tttgctgcgg ctgggcggaa
     2461 acttgaatcg gcggaggcgg gaaggtgatt tcgccgtcgt gggtaacggt catgttgcgg
     2521 ataatcacgt cttcgaagtc caacgtgatt tcgccgtctt tgttcgggct taacagcttg
     2581 gtcaggttga ccaagttggt ggcgtaaagc tgggaagact gtccggcaag gcggtttgcc
     2641 atgtcggtgt agccgatgat tttcacgccg ttgccggtta cggacaattc gcccgggcgg
     2701 gtgagttcgc agttgccgcc cgtcgccgcc gccaaatcga cgatgacgga gccggatttc
     2761 atgctttcca ccatttcttt ggtaatcagc ttgggcgcgg gtttgcccgg aatggcggcg
     2821 gtggtgatga tgatgtccac ttctttcgcc tgctcggcaa agagcttcat ctcggctgcg
     2881 ataaattcgt cgctcatcac tttggcgtag ccgtctccgc tgccgcccga ttcttgtggg
     2941 aagtcgagtt tcaggaactt gccgcccatc gattcgattt gttccgccac ttccaagcgg
     3001 gtatcgaacg cgcgcaccac tgcgccgagc gagtttgccg taccgatcgc cgccaaacct
     3061 gccacacctg cgccaatcac caaaacctgc gcgggcggca ctttgccggc ggcggtgatt
     3121 tggccggtga agaaacggcc gaaggcgttg gcggcttcaa ttacggcgcg gtagccgctg
     3181 atgtttgcca tcgaagacaa agcgtccaaa gcctgcgcgc gcgaaatgcg gggcaccatg
     3241 tccatcgcca gcgcgttcac tttcttggcg cgcaaggctt cgaccaaagc ctcgttttgg
     3301 cgcggccaca ggaagctgac gatggtttga ccttcgttca aaagcggcag ctcgccttcg
     3361 gacggcgcat tgaccttata aatcaaaggg caggcccaaa ccgccgcttt gtcggcaacg
     3421 gtcgcgcctg ctgtttggta agcggcatcg tccaaacttg ccgccaaacc tgcaccgctt
     3481 tcgacaacgg tttcaaagcc cagtttgccc agcagggcga cggtggcggg cgtgcaggcg
     3541 acgcgggttt cgccggataa tgactcgcgt gggataccga ttttcatctc tgaatccttt
     3601 ttcgggtagt ttatatgtat cgtgggttaa atttaaatcg gggcagggcg gagcaacgcc
     3661 gtaccggttt aaaaccgatt cacttcaaat gttaatatat tttagataat ccccttataa
     3721 cgaattttca tcaggctggc aatagttgcg gcattttccc gtgttgtccg acacatactg
     3781 ccgtttgcgt ttgaagtcat tttccgattg cagtcccgcc cgccgtaaca ttctttgccg
     3841 tttcactata taatccgcat tttttgagtt accgccatgc cgcacgccct cgtcctccaa
     3901 tttccctccg ccgaagccct gccttccgac ttcccctcac gcctgcccga acctgattac
     3961 gccgatgaaa agcgtatgcg ttttatcgtt gaagaagggt tttctttaag cgaggcggac
     4021 gcggcgttgc ttggcagccg tcaaatcgac cacgccgtgt tgccggatat ggatttcgac
     4081 gaactcggtt taattgtcag cgatatggat tcgacgctga ttaccattga atgcgtcgat
     4141 gaaattgcgg caggcgtggg tttaaaagac aaagtagcgg aaattaccga gcgttcgatg
     4201 cgcggcgaac tcgatttcga acagtcttta cgcagccgcg ttgcgctgtt ggcgggattg
     4261 gacgaacaga ttttggcgga cgtttatgaa aacgttttga agctctcgcc cggtgcggaa
     4321 ttcttgttgg acgaatgcaa aaggcacgat gtgaaattcc tgctggtgtc cggcgggttc
     4381 acatttttta ccgaaaggct gcaacaacga ctcggcttcg aataccaaca cgccaatgtt
     4441 ttggaaattg aaaacggcag gttgaccggt cgtctgaaag gcagaatcat cgacgcgcag
     4501 gcaaaggcgg atttattgcg cgaataccgc agccgcctcg gattgcagcc gcatcaggtg
     4561 ttggcgatgg gcgacggtgc gaacgatatt ccgatgctca aagaagcggg cataggcgtg
     4621 gcttaccgtg ccaaaccgaa agcgcgggcc gccgccgatg cctgtatcaa cttcggcggt
     4681 ttggagcgtg tacgcggcct gttcggatag gcggatagga aacggatgcc gtctgaaagg
     4741 ttttcagacg gcatttgaac ggcaggaacg acagtgggac gcagaaaact ttggtttgcc
     4801 ctggcagcag cgggcgttat cggcggtttg gtcggcattg tgctgacgga actgatgcac
     4861 ttcatacaac atacggcata cggttatggc acggacggcg tgtccatctc gttccgcgaa
     4921 ggtgtggcac aggcttccgg tatgcggcgc gttgccgtgc tgacgctgtg cggcgcggtc
     4981 gcaggcagcg gctggtggtt gctgaaacgt ttcggcaagc cgcaaatcga aatcaaagcc
     5041 gccttgaaac agccgttgca ggggctgccg tttctgacga cggttttcca tgttctgctg
     5101 caaatcataa cggtcggact cggttcaccg ctcggacggg aagtcgcccc gcgcgaaatg
     5161 accgccgcgt ttgcttttgc cggcggcagg cggttgggtt tggacgaagg cgaaatgcgg
     5221 ctgctgattg cttgcgcttc gggtgcgggt ttggcggccg tgtataacgt gccgctcgcc
     5281 tccacgctgt tcatcctcga agccatgctg ggcgtgtgga cgcagcaagc cgtcgccgcc
     5341 gcattgctga cttccgtcat cgccaccgcc gtcgcgcgca tcggcttagg cgacgtgcag
     5401 caatatcatc ccgccaacct tgccgtcagc acttcattac tttggttttc cgccgtcatc
     5461 ggtccgatac tgggcgtaac cgccgtcttt ttccaacgta ccgcccaaaa attccccttt
     5521 atcaagcgcg acaatatcaa aatcattctc ttggccgtct gtatgtttgc actcgtcggc
     5581 gcggttgccg tttggtttcc cgaagttttg ggcaacggca aagcaggcaa ccaactgacc
     5641 tttggcggct tgaccgactg gcaacacagc cttgagctga ccgccgtcaa atggctggtc
     5701 gtattgatgg cacttgccgc cggtgcatac ggcggcctga ttacgccgtc gatgatgctc
     5761 ggcagcacca tttcttttgc tgccgccgcc gcgtggaaca gcgtcttccc cgaaatgccg
     5821 tctgaaagcg cggcagtcgt cggagcggcg gtgtttctcg gcgtatcgct caaaatgccg
     5881 ctgaccgcca ttgtgttcgt attggaactg acctacgcgc cgctgtccct gctgctgcct
     5941 ttgtgcatcg gcatggcggg ggcattggga acatcgggaa aaatgggctt caaatgaatg
     6001 ccgacaaatg ccgtctgaaa ggttttcaga cggcatttct ttgtaaattg tcaacaatat
     6061 ctgcaaaaac ttactgccaa aaaactgaaa atagcggata ataccgcccc gaaaaagttc
     6121 ccctttcccc atccaactgt cagaaaagga agccccaatg tccgtcatca aacgcgccct
     6181 gatcagcctg tccgacaaga cgggcgcagt cgaatttgcc caaaccctgc acaagctcgg
     6241 tgtcgaaatc ctctctaccg gcggcacggc gaagctgctt gccgatgcgg gcgttcccgt
     6301 gattgaagtt gccgactaca ccggtttccc cgaaatgctc gacggccgcg tgaaaacgct
     6361 gcatccgaaa atccacggcg gtattttggg ccggcgcgat ttgcctgaac acgtcgccaa
     6421 gatggaagaa cacggcatcg gcaacatcga cctcgtgtgc gtcaacctct accccttcgc
     6481 tgccaccatc gccaaaccaa actgcacgct ggaagacgcg attgaaaaca tcgacatcgg
     6541 cggcccgacc atggtgcgct ctgccgcgaa aaactggaaa cacgtcgcca tcgttactga
     6601 cacagccgat ttcccggcca tagctgccga actcgaagcc aacaacggcg cattgagcga
     6661 caaaacccgt ttcaacctgt cgcgcaaagc gttcagccac accgcccaat acgacggcat
     6721 gatttccaac tacctgacct cgctttcaga cgacgttttg agcggagagc ctgaaatcgg
     6781 cgaatttcca agtcggttca accaaagctg gattaaagtg caagacatgc gctacggcga
     6841 aaacccgcat cagcgcgccg cgttctaccg cgatattgac cccgccgcag gcagcctttc
     6901 cgcctacaaa caactgcaag gcaaagaatt gtcgtacaac aacatcgccg atgccgatgc
     6961 cgcatgggaa gccgtcaaat cctttgacgc gccagcctgt gtgattgtga aacacgccaa
     7021 tccgtgcggc gtagctgttg cagccgatac cttgaccgcc tacaaactcg cctacgccac
     7081 cgacaccacc agcgcgttcg gcggcatcat cgctttcaac cgcgaagtgg acggcgaaac
     7141 cgtcaaacaa attaccgaca accagtttat ggaagtcctg atggcgccga agtttaccgc
     7201 cgaagccctc gaaattgcca ccgccaagaa aaacgtgcgc gtgttggaag tgcctttgga
     7261 agcaggcgcg aaccgcttcg aactcaaacg cgtcggcggc ggactgttgg tacaaacccc
     7321 cgacattcac cgcctcaacc gcgccgattt gaaagtcgtt tccaaacgcc aaccgaccga
     7381 gcaagaatgg aacgacctga tgtttgtttg gaacgtcgca aaatacgtca aatccaacgc
     7441 catcgtcttc ggcaaaggcg gtcaaaccta cggcatcggc gcaggccaaa tgagccgcgt
     7501 ggacagtacc cgcatcgccg cccgcaaagc gcaggacgca ggcttggact taaacggcgc
     7561 atgtgccgcc tctgatgctt tcttcccctt ccgcgacggc gtggacgtga ttgccgagca
     7621 gggcatcaaa gccatcatcc atccggcagg ttcgatgcgc gatcaggaag tcttcgacgc
     7681 agcggacgaa cacggcatcg ccatggtcgt aaccgacgtg cgccacttcc gccattaacg
     7741 cagatgaaca aggcaatgcc gtctgaaggg ctttcagacg gcattttgcg ctattttgtg
     7801 aaggtaggga tgacggttcg ggtattcctg acagggtgga ttttcaaggt gttgtatagg
     7861 gtgtaggagg attcgtaaaa ggtgggatgc agggtgtgct tcagcccgct gcatcaaaaa
     7921 tttttggaga atcggcggga gtcggcggtt ttggtttcgg cggggacggt ggaaatgggt
     7981 aacattgacg gaatcgacgg aagcggtggg cttcagccca ccgcttgtat attggaatca
     8041 ccgtatcata gcgccacccg cgcccaccca aggcacacaa ccgttgcgta gctcagggag
     8101 cggcagggca acccatcgac acaaccggac agttgccgga caacacaacc gaatgcaagg
     8161 caggtttatg atgagtaccc aataccatta cgcaggtata gtgaattaaa tctaaaccag
     8221 tacagcgttg gttcgcctta gctcaaagag aacgattctc taaggtgctg aagcaccaag
     8281 tga
//
LOCUS       DAWFLT010000082         8229 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWFLT010000082 DAWFLT010000000
VERSION     DAWFLT010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.082"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..406)
                     /locus_tag="WGR74_001778"
     CDS             complement(<1..406)
                     /locus_tag="WGR74_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0906146.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGVNKNTAAYYFHR
                     LRLLIYQNSPHLEMFDGEVEVDESYFGGQRKGKRGRGAAGKVAVFGLLKRNGKVYTVT
                     VPNTRTATLFPIIREQVKPDSIVYTDCYRSYDV"
     gene            468..578
                     /locus_tag="WGR74_001779"
                     /pseudo
     CDS             468..578
                     /locus_tag="WGR74_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            697..1101
                     /locus_tag="WGR74_001780"
     CDS             697..1101
                     /locus_tag="WGR74_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ0906147.1"
                     /translation="MGSLFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKAAAAG
                     HGILYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGW
                     LLFAAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(1136..2011)
                     /gene="rsmI"
                     /locus_tag="WGR74_001781"
     CDS             complement(1136..2011)
                     /gene="rsmI"
                     /locus_tag="WGR74_001781"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ0906148.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLAGRVREAGFKVVPVVGASAVMAALSVAGVEGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYNLALSWKNK"
     gene            2058..2405
                     /locus_tag="WGR74_001782"
     CDS             2058..2405
                     /locus_tag="WGR74_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ0906149.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGVAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GNRPPEWIQNITG"
     gene            2410..3003
                     /locus_tag="WGR74_001783"
     CDS             2410..3003
                     /locus_tag="WGR74_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ0906150.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAMLKDT
                     DVLLNVPYPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            3067..3675
                     /locus_tag="WGR74_001784"
     CDS             3067..3675
                     /locus_tag="WGR74_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ0906151.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLHQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3683..4327
                     /locus_tag="WGR74_001785"
     CDS             3683..4327
                     /locus_tag="WGR74_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906152.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     TAIGALAIGIVSTFNPNGDKTLQAEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            4367..5146
                     /gene="map"
                     /locus_tag="WGR74_001786"
     CDS             4367..5146
                     /gene="map"
                     /locus_tag="WGR74_001786"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ0906153.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGSVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            5301..5600
                     /locus_tag="WGR74_001787"
     CDS             5301..5600
                     /locus_tag="WGR74_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ0906154.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5838..6212
                     /locus_tag="WGR74_001788"
     CDS             5838..6212
                     /locus_tag="WGR74_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ0906155.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(6400..7866)
                     /locus_tag="WGR74_001789"
     CDS             complement(6400..7866)
                     /locus_tag="WGR74_001789"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ0906156.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSTPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         2137 a         2430 c         1977 g         1685 t
ORIGIN      
        1 atacatcata gctacgataa caatccgtat aaacaatgct gtcaggtttc acttgttcac
       61 ggataatagg aaataaagta gcggttcgag tattcggtac tgtaaccgta taaaccttac
      121 catttcgctt caaaagaccg aatacggcga ctttaccggc agcaccgcga ccgcgtttgc
      181 ctttgcgttg tccgccaaaa taactttcat ctacttctac ttcgccatca aacatttcca
      241 aatgcggact gttttgataa ataagtaatc gtaaacgatg aaaataatag gctgcggtat
      301 ttttattaac gcctactaac tctgctgccg ttcttgcagt tacacctgcg acaaacagtt
      361 caatgagttt attttgttta taccggctta gacgactttt tctcataggg ataattctaa
      421 cttaatttga atttccctag ttatctagga cagcccctaa gttttttatg aacacaaacc
      481 aacctgccgt ttacgacccg ttgacacgta cgctgcactg gctgaccgtt gccggtttca
      541 tcggcattcc gaccaccatt gtcctgtgga cgatttatag tagattaaca aaaatcagga
      601 caaggcgacg aagccgcaga cagtacaaat agtacggcaa ggcgaggcaa cgccgtactg
      661 gtttttgtta atccactata cgaagaggcg gaatgggtgg gcagcctgtt cggcctgcac
      721 aaatctttcg gtttccttac gctgacggtg attacattgc gcatcgtgtg ggcggttgcc
      781 aaccgcgcca agcgtccgca aagcgacagc aaggctgcgg cggcaggaca cggcattctg
      841 tatctgctca tgcttgccgt tcccgttatc ggcatgatcc gccaatacgg cagcggccgc
      901 ggcccgttga aagtgttcgg cgttgaagtg atgcagggtt cgccggaaaa aatcgagtgg
      961 atggcaaact tgggcaacac gttccacggc aatttgggct ggctgctgtt tgccgccgtc
     1021 gtcggacacg tcgccatggt cgtcgtccac cgtgttcaag gcaaagacgt gctgtaccgc
     1081 atgacggggc gtgtccgttg attccgttcg cactatggtg ccggtccgtc cggcatcatt
     1141 tgtttttcca agacagtgcc agattgtaca aagctttttt tccctcgccc gtgattttgg
     1201 cggcaagctc cgccgcctgt ttggtcggca gctcggctgt gaggattttc atgatgtttt
     1261 gcgcggactc ggacaagcct tcgtgttttt catcctgcgc cggataaagc accaacacca
     1321 tctcgccgcg cgattggttg ccgtcggcag acaatgccgt ctgaatttcc ccaaccgtgc
     1381 cgcttaagaa cgtttcaaac gttttcgtaa tttcgcgcgc cagcattaat cggcgttcgg
     1441 ggaacagttc cgccatatcg gcaagcgtcg caccgatgcg gtgcggcgtt tcaaacatga
     1501 cgataggaaa cgccgcccgc acccatttgg caaacagttt cctgcgttct cccgatttcg
     1561 gcggtacaaa accgttgaaa taaaaatcgg atccttccac accggccacg ctcaaagccg
     1621 ccatcaccgc gcttgcgccc acgacgggaa cgactttaaa cccggcctca cgcacgcggc
     1681 cggcgagttt ggcacccggg tcgcacacgg ccggcgtacc cgcatcggaa acctgtgcca
     1741 caaccatgcc gtctgaaaga tagccgacaa tcttgtccgc catctgccgt tcgttgtgtt
     1801 cgcgcacact gacaagtttg ccctgaatgc cgtacgcgct caaaagctgc gcggtaacgc
     1861 gcgtgtcttc ggcacagatg atgtccgcct tttgcaatac cgccaaagcg cgcagggtaa
     1921 tgtccgccaa attgccgatg ggcgtggcaa ccacgtataa tgtccctccg acgacgctgt
     1981 cggaggcttt ctgcaagtgt ttctgaaaca taaaaaatgc cgtctgaaaa acagacatta
     2041 taaaggttaa accgattatg cgcctaaacc acaaacaggg cgaggcaggg gaagatgccg
     2101 cgcttgcctt cctccaatcc caaggctgca cactgcttgc ccgcaactgg cactgcgcct
     2161 acggcgaaat cgacctgatt gtcaaaaacg gcggcatgat tctgtttgtt gaagtaaaat
     2221 accgcaaaaa tcggcaattc ggcggtgtcg catacagcat ttccccatcc aaattattga
     2281 aactgcaacg aagtgtagag tattatctgc aacagaacag gttgacaaac gtaccgtgcc
     2341 gcctcgatgc ggtactgatt gaaggaaacc gcccgcccga gtggatacag aatattacag
     2401 gttgacgata tgacgacatt acaagaacgc gttgccgccc attttgccga aagcatccgt
     2461 gccaagcagg aagccggaaa agtattggtc gagccgaccg tacaggctgc cgagctgatg
     2521 ctgcaatgcc tgatgaatga cggcaaaatc ctggcctgcg gcaacggcgg ttcggctgcc
     2581 gacgcgcaac acttcgccgc cgaaatgacc ggccgttttg aaaaagaacg catggaactc
     2641 gccgctgtcg cgctgacaac agacacttcc gcgctgacag ccatcggcaa cgactacggt
     2701 ttcgaccacg tattcagcaa acaggtacgc gccctcggac gtgcaggcga cgtcttggtc
     2761 ggcatttcca cttccggcaa ttccgccaac gtcatcgaag ccgtcaaagc cgcacacgaa
     2821 cgcgatatgc acgtcatcgc cctgaccggc cgcgacggcg gcaaaatcgc cgccatgctc
     2881 aaagacaccg acgttttact caacgttccc tatcctcgaa ctgcccgtat tcaggaaaac
     2941 cacatcctgc tgatacacgc catgtgcgac tgtatcgact ccgtactgct ggaaggaatg
     3001 taaccctttt cagacggcat ggcgcaaagc aatgccgtct gaaacgccca agaaaggaag
     3061 cccccgatga aacccaaacc gcacaccgtc cgcaccctga ttgccgccat tttcagcctt
     3121 gcccttagcg gctgcgtcag cgcagtaatc ggaagcgccg ccgtcggcgc gaaatccgcc
     3181 gtcgaccgcc gaaccaccgg cgcgcaaacc gacgacaacg ttatggcgtt gcgtatcgaa
     3241 accaccgccc gttcctacct gcaccaaaac aaccaaacca aaggctacac gccccaaatc
     3301 tccgtcgtcg gctacaaccg ccacctgctg ctgctcggac aagtcgccac cgaaggcgaa
     3361 aaacagttcg tcggtcagat tgcacgttcc gaacaggccg ccgaaggcgt gtacaactac
     3421 attaccgtcg cctccctgcc gcgcactgcc ggcgacatcg ccggcgacac ttggaacaca
     3481 tccaaagtcc gcgccacgct gttgggcatc agccccgcca cacaggcgcg cgtcaaaatc
     3541 gttacctacg gcaacgtaac ctacgttatg ggcatcctca cccccgaaga acaggcgcag
     3601 attacccaaa aagtcagcac caccgtcggc gtacaaaaag tcatcaccct ctaccaaaac
     3661 tacgtccaac gctgactcgg caatgccgtc tgaaccgcct tcagacggca ttgcccgaca
     3721 ccccaaaagc acaatcaaaa tggcaaaaaa accgaacaaa cccttcaggc tgacccccaa
     3781 actcctgata cgcgccgtat tgctcatctg tatcaccgcc atcggcgcat tggcaatagg
     3841 catcgtcagc acattcaacc cgaacggcga caaaaccctt caagccgaac cgcaacacac
     3901 cgacagcccc cgcgaaaccg aattctggct gccaaacggc gtagtcggac aagatgccgc
     3961 ccaacccgaa caccaccacg ccgcctcatc cgaacccgca cagccggacg gcacagacga
     4021 aagcggcagc ggactgccgt cccctgccgc acccaagaaa aaccgggtca aaccgcaacc
     4081 tgccgacaca gctcaaaccg acaggcagcc ggacgacgcc ggaacacaag ctgaaaacac
     4141 actcaaagaa acccccgtac tgcccacaaa cgtcccccgt cccgaacccc gaaaagaaac
     4201 acccgaaaaa caggcgcagc ccaaagaaac gcccaaagaa acgcccaaag aaaaccatac
     4261 caaaccggac accccgaaaa acacgccgcc caaaccccat aaagaaattc tcgacaacct
     4321 cttctgaccc ggcacggcag gcacacccgc aatccaagga agcattatga acggcatcat
     4381 catcaaaacc cccgaagaaa tcgaaaaaat gcgcgagctg ggcaaactcg tcgccgaagc
     4441 cctcgactac atcggacaat tcgtcaaacc cggcgtaacc accgacgaaa tcgacaaact
     4501 cgtttacgac taccacgtca acgtccaagg cggctatccc gcccccctgc actacggcaa
     4561 cccgccctac cccaaatcct gctgcacctc cgtcaaccac gtcatctgcc acggcattcc
     4621 cgacgacaag ccgctcaaag aaggcgacat tatcaacatc gacctcacca tcaaaaaaga
     4681 cggcttccac ggcgactcca gccgtatgtt taccgtcggc aaagtctccc ccatcgccca
     4741 acgcctgatc gacgtaaccc acgcctccat gatggcgggc atagaagccg tcaaacccgg
     4801 cgcgacactg ggcgacgtag gttacgcctg ccaacaggtt gccgaaaacg ccggctattc
     4861 cgtcgtacag gaattctgcg gacacggcat cgggcgcggt ttccacgaag ccccgcaagt
     4921 gttgcactac ggaaaaaaag gacagggctc cgttctaaaa ccgggtatga tttttaccgt
     4981 cgaaccgatg atcaaccaag gcaaacgcca cctgcgtatc ctcaacgacg gctggacggt
     5041 ggttaccaaa gaccgctccc tctccgccca atgggaacac gaagtcttgg tgaccgaaac
     5101 cggctacgaa atcctcaccg tcagccccgc ctccggcaaa ccctgaagcc ggacgtatcc
     5161 gccccataaa aacaaacaat gccgtctgaa agaaacggca gatatgatat ataatataaa
     5221 aacaggcttg acccggcaca ttacgaaaac aaagcaaatc ggaatttgcc ccgcaaccag
     5281 acaaacttaa aggaagtttt atgaaaatat ttgaaaatat agaagatgtt aaagccatcc
     5341 gtaaaaagac cgggctgaac cagatagact tctggggcaa ggtcggcgtt acccagtctg
     5401 gaggctcgcg ctacgaaacc ggccgcaaaa tgcccaaacc cgtacgcgaa ctgctccgcc
     5461 tcgtccatat cgaatgcatc gatttggcga aagtcaacaa aaaagatatg gaaatcgccg
     5521 ccctgttgaa aaaacaccat cccgacctgt atgccgagtt gtccaaacag accaagtccg
     5581 aaagaaaaaa acaaagttaa accgcaacct ccggatgccc gacagttttt catttccgaa
     5641 aaacgcaaac aatgccgtct gaaacaccgg acaggtcgcc gtatcccgcc tgccgcccct
     5701 gcctcaaacc gccgaaccgc ccgaacccgc ctttttacaa actttatcca atttcctgtt
     5761 tatttcagga tacgccgaca ttagaatgtc aaacagctcg aaacgggcaa actccacatc
     5821 catcccaagg aataaaaatg aaacttctga ccaccgcaat cctgtcttcc gcaatcgcgc
     5881 tcagcagtat ggctgctgct gccggcacga acaaccccac cgttgccaaa aaaaccgtca
     5941 gctacgtctg ccagcaaggt aaaaaagtca aagtaaccta cggcttcaac aaacagggtc
     6001 tgaccacata cgcttccgcc gtcatcaacg gcaaacgtgt gcaaatgcct gtcaatttgg
     6061 ataaatccga caatgtggaa acattctacg gcaaagaagg cggttatgtt ttgggtaccg
     6121 gcgtgatgga tggcaaatct taccgcaaac agcccattat gattaccgca cctgacaacc
     6181 aaatcgtctt caaagactgt tccccacgtt aatcaggcaa caaaaaacag cgttttcaga
     6241 aatgaaaacg ctgttttttt gaccgttcca ttattcgcaa aagggaaaaa acgattacct
     6301 gccccgtgta tcaaaacctg ccctgccgga tgaagggcat aaccggcagg gacggcatca
     6361 acaccatatg ggggtacggc ttttcttgaa agattcggct taaatatcca acactttcgc
     6421 ggtataggcg ataatttcat ccgccctttc ggggttttcg ttcaacttga tgccgtaacc
     6481 cggtaccagc tctttcagac ggccttccca agacggggcg cgctcgggga agcattggtg
     6541 catcagccgg atcatcagcg gcacagcagt cgatgcgccc ggcgacgcgc ccagcaatgc
     6601 ggcgagtgag ccgtcggcgt gggcgacaat ctccgtacca aactggagca cgccgccttt
     6661 ttcggagtct tttttaatga tttggacgcg ttgccctgcg gtgatgagtt cccagtcgtc
     6721 ggggtttgcc tcggggtagt attccagcag ggaggcgaag cgttcttctt tggttttacg
     6781 caattcgccc agcaggtatt tggtcagcgg catattcgcc cagccggcgc acagcatagg
     6841 atagaggttg tccatatgga tggacagcgg caaatccata agcgagcctt gcttgaggaa
     6901 gttggaacgg aagcctgcgt aagggccgaa cataaggtgg cgtttgccgt ccacgttgcg
     6961 tgtgtcgagg tgcgggacgg acatcggcgg cgcgccgacg gaagcctgcc cgtacacttt
     7021 ggcgttgtgt tgttcggcgg tttcaggggt gctgttgcgg aagaacaggc cggacacggg
     7081 gaagccgccg tagcctttgc cttcggggat gccggatttt tgcagcaggg tcagcgcgcc
     7141 gccgcccgcg ccgaggaaga gaaagcgggt acggagggtg agctgcccgt cggggttgcg
     7201 ggtatcggcg gttttgagca cccacgcgcc gtcggattcg cgtttgatgt cttcgacgtg
     7261 gcggttgaac tcggttttta cgcccttgcc ctgcaaatat ttcaccattt ggcgcgtcag
     7321 ccgtccgaaa tcgacatccg tgccttcggc ggagtagttg gcggcgacgg gttggttttc
     7381 gtcccggccg cgcatcatca gcggagccca atcggaaatt ttgttccgat cggtggaaaa
     7441 ttccatattt tcaaaaagtt tttgggtttt aaacgcgtca taacgttttt gaagataaga
     7501 acaatggtct tcattcatca ccaaagacat atgcggcacg gcattgatga aggaattgtc
     7561 ttccaacttg ccttccgcga ccaacgtcgc ccaaaactgg cggctgacat gaaactgttc
     7621 ggcaatattg agggcgcgcg ccggatcgat aatcccattt gcacccaacg gcgcatagtt
     7681 caattcgcac agcgcggaat gccccgtgcc ggcgttgttc cacgcgtttg acgattccaa
     7741 cgccacatct tccaagcgtt caatcagggt gatttcccaa gacggttcga gttctttgag
     7801 caaaacgccc aaagtcgcgc tcataatgcc gccgcccacc aagacaacgt ctgtcgcttc
     7861 agccatggtt tactcctaaa aaacaggcat cttctgccct tatggttatt tgccgtacta
     7921 caaacgcctg aatcgcaaaa gcagggaaaa ccggcaatgg tgtgtgtccg agtatgctgt
     7981 ttcagggttg gaatgcgttg caagtatggc ttccgacacc gcttcagggg cttgtaatat
     8041 gttatcgtga atgtagtgga ttttactggg aaatgcaaag tttttctgtc gcccgccaag
     8101 cccgggaact gcgaaatgaa aaataaaaat agttatttat ctatatatat caaattttta
     8161 atagataaaa aaccaaaatt gtttatatat taatttttaa aagattgtca gcatattgcg
     8221 ttaagtttt
//
LOCUS       DAWFLT010000083         8197 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWFLT010000083 DAWFLT010000000
VERSION     DAWFLT010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8197
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.083"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..398)
                     /locus_tag="WGR74_001790"
     CDS             complement(<1..398)
                     /locus_tag="WGR74_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0906157.1"
                     /translation="MKITHCKLKKKVQKELIRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEGSVEPDKSDFGGRRKGRRGRGAAGKVVVFGILKHNGRV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTD"
     gene            complement(631..2688)
                     /gene="ppk1"
                     /locus_tag="WGR74_001791"
     CDS             complement(631..2688)
                     /gene="ppk1"
                     /locus_tag="WGR74_001791"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ0906158.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGAQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            complement(2857..3300)
                     /locus_tag="WGR74_001792"
     CDS             complement(2857..3300)
                     /locus_tag="WGR74_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ0906159.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDAVNAYIQKKKVIVNTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            complement(3307..3822)
                     /locus_tag="WGR74_001793"
     CDS             complement(3307..3822)
                     /locus_tag="WGR74_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0906160.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            4092..6824
                     /locus_tag="WGR74_001794"
     CDS             4092..6824
                     /locus_tag="WGR74_001794"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ0906161.1"
                     /translation="MQEQYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYSLPKKQVLQRVTIGLSPEQEEAMGFGGGSGC
                     GAGYGDGSGSDSRDLPFMPNKWQAWYGVKDDFMVLINSGELDGMNYQTAFDAVAAKLQ
                     AIGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCEQCGDVPVPADQLPVVLPENVVPDG
                     MGSPLAKMPEFYETTCPCCGGAAKRETDTMDTFMESSWYFFRYMSPKFSDGMVDPAAA
                     KYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEGLVNVDEPFERLLTQGMVVCETYYR
                     ENDKGGKDWINPADVELTFDDKGRPVSAVLKVDGLPVVISGTEKMSKSKNNGVDPQEL
                     INAYGADTARLFMMFAAPPEQSLEWSDSGVEGAHRFLRRLWRTVYEYLKQGEAVNAFA
                     GSQDGLSKELKDLRHKLHSTIAKVSDDYGRRQQFNTAIAAVMELLNQYDKTDTGGEQG
                     RAVAQEVLEAAVRLLWPIVPHICETLWSELNGAKLWEAGWPTVDEAALVKSEIEVMVQ
                     VNGKLRGKITVAADTSKADLEASALATEGAVKFMEGKPAKKIIVVPGRLVNIVV"
     gene            7056..7847
                     /locus_tag="WGR74_001795"
     CDS             7056..7847
                     /locus_tag="WGR74_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0906162.1"
                     /translation="MNLFFDTELGRQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPNHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYEFEKKLSIQ
                     FPQNNHIRDKIRQQLQILRDQNTIEFIGRGLYKKIDKLPPTSKAF"
     gene            7977..8090
                     /locus_tag="WGR74_001796"
     CDS             7977..8090
                     /locus_tag="WGR74_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HEZ0906163.1"
                     /translation="MRDSDKPAASEGAVKFIEGNPAKKIIAVPGRLASIVV"
BASE COUNT         1942 a         2132 c         2255 g         1868 t
ORIGIN      
        1 ctatcggtat aaacaatgct gtccggcatg attttcttct tgatgacagg gagtaacgtt
       61 tcagacttgg cattatccac cacaacggta tagacccgtc cgttgtgttt cagaatgccg
      121 aagacaacca cttttcctgc cgcaccgcga ccacgtctgc ctttacgccg tccgccgaaa
      181 tcgcttttgt ccggctcgac agagccctca aaaacctcat cggcagccaa ggccaaatga
      241 tggttgataa ccgtgcggat tttacggtag aacagtgctg ccgaattggg atggataccc
      301 aaaatatcgg cggcagaacg ggcggtaact tcgagtacaa aaaaacggat cagttctttc
      361 tgtacttttt tctttaattt gcagtgtgtt atcttcatat tcccagggta acatatctgc
      421 taatctagta cagcccctaa aatttaatcc actataatgc cgtctggaat gcggcggctt
      481 ggggttggga gcgtataggg gaagtgcttg tgcaggaatg gcgaaggtgt gtttttgact
      541 ccggtccgcc ctgtttagat tgctgtaaaa ctatgccgtc tgaaacggaa aggtttcaga
      601 cggcatggtt tttgattggg gcggtgcggg tcagcctccg agcagtgtcc acaaatcgtt
      661 ttgcaggtcg gcttcggatt cgccctcggc gggcgcggcg cggatatagc cgccgtcggg
      721 ctgcatcagc cacgcgtgcg tattgtctgc caatgccatt tccaaacctt cgcggatgac
      781 gcgttttttg agttcgggcg cggtaatcgg cgcggcggtt tcgatgcggc ggaagaagtt
      841 gcgccccatc caatccgcgc tggagataaa ggtatcgtcc gcgccgttgt tgtggaagca
      901 atacacgcgc gcgtgttcga gctgcctgcc gatgatggag cggacgcgga tgttttcgga
      961 caagcctttt acacccgggc gcaaggtgca cataccgcgc acaatcaaat cgatttgtac
     1021 gcctgccgcg cttgcccgat acagggcttc gatgacggtc ggttcgatga gcgaattcat
     1081 cttggcggta atccgcgccg gtttgccggc ttttgcgtgt tcggtttcgc gtgcgatgcg
     1141 gtcgataacc attttgtgca gggtaaacgg gctttggtag agtttgttca gccgcccggg
     1201 tttgcccaag cctgtgattt ccataaacaa tgtgttcaca tcggcggtga tttgttcgtc
     1261 ggcggtaatg atgccgaagt cggtgtagat gcgcgatgtg ccttggtggt agttacccgt
     1321 gccgaggtgg gcgtaacgct tgagtacgcc gtcttcgcgg cggatgacca gtgccatttt
     1381 ggcgtggact ttgtagccga acacgccgta cacgacgtgc gcgcccgcct cttcgagctg
     1441 cttcgcccag ttgacgttgt tggcttcgtc aaaacgcgcc atcagttcga cgacgacggt
     1501 tacttgtttg ccggcgagtg ccgccttcat cagggcgcgg acgagttcgg aacgcgtgcc
     1561 ggtgcggtaa atcgtcattt tgacggcaag cacgtcgggg tcggcggcag cttcgcgcat
     1621 catttcgaca acggggtcga acgattggta ggggtggtgc agcaggatgg gcgattggcg
     1681 caccaaatcg aatatggacg cggttttgcc caaggctttc agtctgcccg gcgtgtgtgt
     1741 gggaaatttc aaatcggggc ggttgactag gtcggggacg gcgttgaggc gcacgaggtt
     1801 gaccgggcct ttgacctgat agagttcggc ggcggtcagt ttgaattgcg cgagcagaaa
     1861 gtcgcggatg taggcgggac aggtgtcggc gacttcgagc cgcacgccgt cgccgtattc
     1921 gcggtcgtgc agttcgtttt ggatggcggc gcggaggttt tgcaggtctt cttcgtcaac
     1981 ggtcaagtcg ctgtcgcgcg tcaggcggaa ctggtggcag cctttgacgt tcatgcccgg
     2041 gaagagtttg ccgacgtggg cgtggaggat ggacgacagg aagacgaagc cgtgtccgcc
     2101 gccacacagt tcggacggca ggggaacgac gcgcggcagg atgcgcggtg cctgcacaat
     2161 cgccatcccc gaaggcctgc cgaatgcgtc cgtgccgtcg agttcgacgg cgaagttgag
     2221 cgatttgttc agcggtcgcg ggaaggggtg ggacgggtcg agtccgatgg gggtcaggat
     2281 cggcagcagt tcgcggtcga aatagtcttc gatccatttt ttctgcgcgc ccgtccagtt
     2341 gcggcggcgg taaaaatgga tgctttcccg cgccaactcg ggctgaagga cgttgttgaa
     2401 caggtcgtac tggtgccgta tcagggagcg cgccgcttcg gtaacgtcgg cgatggtttc
     2461 agacggcatt ttgccgttgt ccagcctgcg ccgggggtgc agcttgtttt cacgcttgag
     2521 ccacgccatg cggacttcga aaaactcgtc aagattggac gacacgatgc acaggaagcg
     2581 caggcgttcc aaaaggggga cgtttttgtc ttccgcctgt gccaacacgc ggcggttgaa
     2641 tgccagcagg cttagttcgc ggcagaggat gcggttttgt tcgggcataa ggttctccca
     2701 aagatggttg ttgttcggtc ggagcgggca ggcaatgccg tctgaaggct tggcggcatc
     2761 tgcgccggtt tcagacggca tgacgtggcg gacagcctgt ttcaggcatc ggggcaatgt
     2821 gaaaacagat accggcgcgt gcggtatccg tctgtttcaa atcacttcca acacaaaata
     2881 atgacgcagt ttttcgtaca ccgcatcgct gacgttgatg cagccgttgg tcataatcct
     2941 gtcggacacg gacggggagg cgatgcgttc gttccgcctt tccgacggta tctgattcca
     3001 aacgcggtgc agggcgaaaa gaaaatcacc ctcctgcttg aaaccgatga cttcgccgcc
     3061 gtatccaggc ttgtcggtac tgttcagcgt cagcccgaac gtccccttgg gcgtggcaac
     3121 accgatgagg acggggtggc actgcctgtc atcggcaaaa cagagttccg ctttggatgt
     3181 gtttacgatg acttttttct tttgaatata agcgttgacc gcatccggtt gcccctgtgc
     3241 atatacgggg gcaaccattg acaggccgca taatatccct aaaaacaggc ggaacggcat
     3301 gtcggattat tggcggatac gtttcggttt tgccggcacg atttcgcgga tgatgacttg
     3361 cggctcggct ttgggtgcgg gcggcggaca ggcggcatct ttcgggaaga cggggttcca
     3421 gtagaagctg cgggcaaatt tgtctttatc gaaaatcact ttgtattggc aggtagtaac
     3481 atcttctacg ccggaagtgt tttcagggtc gatacctacg cccggggtat ggaagtggaa
     3541 caggtaatcc cattcgcgca cgccgtacat accttcgtcg taatgcgggc ggcccaggat
     3601 tttgtagatg tcgtctttgg tcaggccggg gcgcatctga tccagttcgt cataagtcgg
     3661 gaatgtgccg cgcttgttgt cgagcgttac ggaatagggt ttcgggaaaa ccggattgtc
     3721 ggtcgtgccg tcggctttga cgttgctttt ggttgcgcag gcagacagaa cgcccgccgc
     3781 caatactgcc aagcccggtt tgacgatttg tttgattttc atatgcagaa tcctctttta
     3841 cccgatgccc gtctgccttt tgttcggaag agccgcatca ggagagtttt aaacatttga
     3901 ttttggtttg taatattagc ataaaaaacg tgcgtatcaa taaacgcggt gtatttgtac
     3961 ggcatacgga ataatgcgcg tgcgaattta cgcatcctgc cggcaatctg ccgattcgcc
     4021 gcttccggca acctgttata attgatgccg actttatttt tccaacgttt ttaagcgaaa
     4081 acagaatgac tatgcaagaa caatatcaac ccgccgccat cgagcctgcg gcgcagaaaa
     4141 aatgggacga cgcccgtatt ttcaacgtct ccgaagacgc ttccaaaccc aaatattatt
     4201 gcctctccat gttcccctat cccagcggca agctgcatat ggggcatgtg cgcaactaca
     4261 ccatcggcga tgtattgagc cgcttcaaac ttttaaacgg cttcaacgtc atgcagccta
     4321 tgggttggga cgcgttcggc atgccggcgg aaaacgcggc gatgaaaaac aacgtcgccc
     4381 ctgccgcttg gacgtacgat aacatcgaat acatgaaaac ccagctcaaa agcctgggtt
     4441 ttgcgattga ctgggcgcgc gaaaccgcaa cctgcaaacc cgaatactac cgctgggaac
     4501 aatggctgtt taccaagctg tttgaaaaag gcatcgtcta tcgcaaaaac ggcacggtaa
     4561 actgggaccc ggtcgaccaa accgtccttg ccaacgagca agttatcgac ggacgcggct
     4621 ggcgttccgg cgcattgatc gaaaaacgcg aaatcccgat gtattacttc aaaatcacgg
     4681 attacgccga agaactgctc aacgacttgg acaagctgga acactggccg gaacaagtca
     4741 aaaccatgca gcgcaactgg atcggtaaat ctcgcggtat gaccgtgcgc ttcgccgttt
     4801 cagacgacag caaacaaggc ttggaaggcg attacgcgaa attcctgcaa gtttatacca
     4861 cccgtcccga cacgctgatg ggcgcgactt atgttgccgt tgccgccgag catccgctgg
     4921 caaccgccgc agccgccgac aaacccgaat tgcaggcatt tattgccgaa tgcaaagccg
     4981 gttcggttgc cgaagctgat atggcgacga tggagaaaaa aggcgtgccg accggccgct
     5041 acgtcgtcaa cccgctcaac ggcgacaagc tggaagtgtg gattgccaac tatgtattgt
     5101 ggggctacgg cgacggcgcg gtaatggcgg ttccggcgca cgacgaacgc gatttcgagt
     5161 tcgccgccaa atacagcctg ccgaaaaaac aggtcttaca acgcgtaaca atcggacttt
     5221 cgccagagca agaagaagct atgggttttg gtggcggttc cggatgtggt gctggttatg
     5281 gtgatggtag tggttccgat tcaagagatt tgccgtttat gcctaataaa tggcaagcat
     5341 ggtatggagt caaagatgac ttcatggtct taatcaactc aggtgaactg gacggcatga
     5401 attatcagac agcatttgat gctgtcgccg ctaaattaca agctatcgga gctggcgaac
     5461 cgaaaaccca ataccgcctg cgcgactggg gcatttcgcg ccaacgctac tggggctgcc
     5521 cgattcccat cgtccattgc gaacaatgcg gcgacgtacc cgtccctgcc gaccaattgc
     5581 cggtcgtttt gcctgaaaac gtcgtacccg acggcatggg ttcgccgctg gcaaaaatgc
     5641 ccgagtttta cgaaaccacc tgcccatgct gcggcggcgc ggcaaaacgc gaaaccgaca
     5701 ccatggacac cttcatggaa tcaagctggt acttcttccg ctacatgtcg ccaaaatttt
     5761 cagacggcat ggtcgatcct gccgccgcga aatactgggg cgcggtcgac caatacatcg
     5821 gcggcatcga acacgcgatt ttgcacctct tgtacgcgcg tttcttcacc aaactgatgc
     5881 gcgacgaagg tttggtcaat gttgacgaac cgtttgaacg cctgctgacg caaggcatgg
     5941 tcgtttgcga aacctactac cgcgaaaacg acaaaggcgg caaagattgg atcaaccccg
     6001 ccgatgtcga gctgactttt gacgacaaag gccgccctgt ttccgccgtc ctgaaagtcg
     6061 acggactgcc cgtcgtcatc agcggcacgg aaaaaatgtc caagtccaaa aacaacggcg
     6121 tcgatccgca agaactgatt aacgcctacg gcgcagacac cgcccgcctg ttcatgatgt
     6181 tcgccgcacc gcccgaacag tccctcgaat ggagcgacag cggcgtcgaa ggcgcgcacc
     6241 gcttcctgcg ccgcctgtgg cgtaccgttt acgaatactt gaagcaaggc gaagcagtca
     6301 acgcgttcgc aggcagccaa gacggtttgt ctaaagaact caaagacctg cgccacaaac
     6361 tgcattccac tatcgccaaa gtcagcgacg actacggccg ccgtcagcag ttcaacaccg
     6421 ccatcgccgc cgtgatggaa ctgctcaacc aatacgacaa aaccgacacc ggtggcgaac
     6481 aaggccgcgc cgtcgcccaa gaagtattgg aagccgccgt acgcctgctg tggcccatcg
     6541 tgccgcacat ctgcgaaacc ctgtggagcg aattgaacgg tgcgaaactg tgggaagcag
     6601 gctggccgac agtcgacgaa gccgccttgg tcaaatccga aatcgaagtc atggttcaag
     6661 tcaacggcaa actgcgcggc aaaatcaccg tcgccgccga cacctccaaa gccgacctcg
     6721 aagcctccgc actcgccacc gaaggcgcgg tgaaattcat ggaaggcaag cctgccaaga
     6781 aaatcatcgt cgtaccgggc agactggtga acatcgtggt gtaaaaccga ttcggcattg
     6841 actgctgtaa ttaggaaagg ccgtctgaaa cttggaaaca gggtttcaga cggccttttt
     6901 aaggcagatg gagttgtccg catgacagac ctcaaatata gcggattaac tttaaaccgg
     6961 tacggcgttg ccccgccttg ccctgctgtc tgcggcttcg tcgccttgtc ctgatttttg
     7021 ttaatccact caaacttaat ctcaaaatcc aaaacatgaa tttatttttc gataccgaat
     7081 tgggaaggca acaaaataaa gcaacccaca aaatccgtgt aatgagcgag gcttggctgg
     7141 aaaaaaacgg ctactgcccc tgttgcggaa gcaagccgat gcagagattt gccaataaca
     7201 aacctgttgc agacctcttt tgcccaaatt gccacgagca atatgaatta aagagtaaaa
     7261 atcaaaaaac cataggcaac agcgtgcctg acggtgcata tcgcaccatg ctggagcgca
     7321 tccggtcaga taccaacccc aactttttct ttcttgcata taaaaaagcg gattactcca
     7381 tacggcaatt ggtgcttgta cccaaacatt tcatcacgcc ggatatgatt attcccagaa
     7441 ataaaggtat taaaaaccgg ccgaaccaca ttatgtgttc catcaatctc gcccctttgc
     7501 ctgaaagcgg aaaaatattc ttaatagacg attcccgcat tatcgaaccc gaaaccgttc
     7561 tgaaaaaatg gcaatccaac ctgtttttac gcaaccaaaa tgcggagcgc aaaggctggc
     7621 ttttggctgt tatgaaatgt atcgaccaac tccccgaaga attcacattg tcacaaatgt
     7681 atgaatttga aaaaaaacta tccatccaat ttccccaaaa caaccatatc agagacaaaa
     7741 tccgccaaca gttgcaaatt ttgcgcgacc aaaatacgat cgaattcatt ggtcgcggac
     7801 tttacaaaaa aatcgacaaa ttacccccga cttccaaggc gttttaattt caaatcatga
     7861 tactgaattc acaagggata tttattcttc tgctattctg aaaacagttg aagttcagcg
     7921 caatattgcc gcaaaaggca gcagccgtaa aaaagtcggg gaattattgg caatttatgc
     7981 gtgattccga caaaccggcc gcctccgaag gcgcagtgaa attcatagaa ggtaatcctg
     8041 ccaagaaaat catcgccgta ccgggcagac tggcgagcat cgtggtgtaa atctttttaa
     8101 aaacatcaaa caatgttaag atgccgtccg aaatccgttt tagattcaga cggcattttt
     8161 taaatttaat tttttaaggg gtaagcctaa tatgaaa
//
LOCUS       DAWFLT010000084         8151 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWFLT010000084 DAWFLT010000000
VERSION     DAWFLT010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8151
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.084"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            1..2045
                     /locus_tag="WGR74_001797"
                     /pseudo
     CDS             1..2045
                     /locus_tag="WGR74_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            2287..3753
                     /locus_tag="WGR74_001798"
     CDS             2287..3753
                     /locus_tag="WGR74_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0906164.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFEDRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNSGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(3954..4922)
                     /locus_tag="WGR74_001799"
     CDS             complement(3954..4922)
                     /locus_tag="WGR74_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906165.1"
                     /translation="MKLTLAIPSLNLDEDETRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSEAVALATPVWQKMGMHQANVLTAEYLNVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPCAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDSLHGSSQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAYLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRIGKIKRLDITTDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            5000..6292
                     /locus_tag="WGR74_001800"
     CDS             5000..6292
                     /locus_tag="WGR74_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ0906166.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLIYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            complement(6795..7481)
                     /locus_tag="WGR74_001801"
     CDS             complement(6795..7481)
                     /locus_tag="WGR74_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ0906167.1"
                     /translation="MYAFTAAQQQKALFWLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
     gene            complement(7556..8029)
                     /gene="queF"
                     /locus_tag="WGR74_001802"
     CDS             complement(7556..8029)
                     /gene="queF"
                     /locus_tag="WGR74_001802"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ0906168.1"
                     /translation="MSRNTEELQGISLLGNQKTRYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     regulatory      complement(8033..8077)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         1934 a         2018 c         2446 g         1753 t
ORIGIN      
        1 gtcgggttaa tctattaaca ttatctgttt tatcgctgtt ttgcacgcca tatgtttgag
       61 gttcggatgc gtacgatccc gtcaaagaag ccgagattaa aaacaaattt attttagaag
      121 cggcggaaga cagaaattcc cacgtttggc gcggcccgtg cagcatatct tttgattgct
      181 tcggtatgtt cagagctcag cttggttcaa atactcgttc taccaaaatc ggcgacgatg
      241 ccgatttttc attttcagac aagccgaaac ccggcacttc ccattatttt tccagcggta
      301 aaaccgatca aaattcatcc gaatatgggt atgacgaaat caatatccaa ggtaaaaact
      361 acaatagcgg catactcgcc gtcgataata tgcccgttgt taagaaatat attacagata
      421 cttacgggga taatttaaag gatgcggtta agaagcaatt acaggattta tacaaaacaa
      481 gacccgaagc ttgggaagaa aataaaaaac ggactgagga ggcgtatata gaacagcttg
      541 gaccaaaatt tagtatactc aaacagaaaa accccgattt aattaataaa ttggtagaag
      601 attccgtact cactcctcat agtaatacat cacagactag tctcaacaac atcttcaata
      661 aaaaattaca cgtcaaaatc gaaaacaaat cccacgtcgc cggacaggtg ttggaactga
      721 ccaagatgac gctgaaagat tccctttggg aaccgcgccg ccattccgac atccatatgc
      781 tggaaacttc cgataatgcc cggatccgcc tgaacacgaa agatgaaaaa ctgaccgtcc
      841 ataaagcgta tcagggcggc gcggatttcc tgttcggcta cgacgtgcgg gagtcggacg
      901 aacccgccct gacctttgaa gacaaagtca gcggacaatc cggcgtggtt ttggaacgcc
      961 ggtcggaaaa tctgaaaacg ctcgacgggc gcaaactgat tgcggcggaa aaggcagacc
     1021 ctaattcgtt tgcgtttaaa caaaattacc ggcagggact gtacgaatta ttgctcaagc
     1081 aatgcgaagg cggattttgc ttgggcgtgc agcgtttggc tatccccgag gcggaagcgg
     1141 ttttatatgc ccaacaggct tatgcggcaa atactttgtt tgggctgcgt gccgccgaca
     1201 ggggagacga cgtgtatgcc gccgatccgt cccgtcaaaa attgtggctg cgcttcatcg
     1261 gcggccggtc gcatcaaaat atacggggcg gcgcggctgc ggacgggcgg cgcaaaggcg
     1321 tgcaaatcgg cggcgaggtg tttgtacggc aaaatgaagg cagccggctg gcaatcggcg
     1381 tgatgggcgg cagggccggc cagcacgcat cagtcaacgg caaaggcggt gcggcaggca
     1441 gttatttgca tggttatggc gggggtgttt atgctgcgtg gcatcagttg cgcgataaac
     1501 aaacgggtgc gtatttggac ggctggttgc aataccaacg tttcaaacac cgcatcaatg
     1561 atgaaaaccg tgcggaacgc tacaaaacca aaggttggac ggcttctgtc gaaggcggct
     1621 acaacacgct tgtggcggaa ggcgttgtcg gaaaaggcaa taatgtgcgg ttttacctgc
     1681 aaccgcaggc gcagtttacc tacttgggcg taaacggcgg ctttaccgac agcgagggga
     1741 cggcggtcgg actgctcggc agcggtcagt ggcaaagccg cgccggcatt cgggcaaaaa
     1801 cccgttttgc tttgcgtaac ggtgtcaatc ttcagccttt tgccgctttt aatgttttgc
     1861 acaggtcaaa atctttcggc gtggaaatgg acggcgaaaa acagacgctg gcaggcagga
     1921 cggcactcga agggcggttc ggcattgaag ccggttggaa ggattatttg tctgcgcgtc
     1981 tcggatacgg caaaagatcg gacggcgata aagaagccgc attgtcggtc aaatggctgt
     2041 tttgatgcgt cgggaaatgt tttgacgcac aggcggcaca ccggcacggc cccgcgcgcc
     2101 gccccgcaaa ccaatccgaa ccctgccgcc ccgaagggcg gggcatcatg atgaaaccgg
     2161 cggaaaaccg ccggtttttt gccgccgttt gaaacccgat tctggcttca gacggcattg
     2221 tcgcggcatc gggcggcagg gtttggaaca gcggcataaa aaactgatac aatccgccga
     2281 ttgataatgg ttatttttta tttttgtggg aagacattta tgcctgcacg aaacagatgg
     2341 atgctgctgc tgcctttatt ggcaagcgcg gcatatgccg aagaaacacc gcgcgaaccg
     2401 gatttgagaa gccgtcccga gttcaggctt catgaagcgg aggtcaaacc aatcgacagg
     2461 gagaaggtac cggggcaggt gcgggaaaaa ggaaaagttt tgcagattga cggagaaacc
     2521 ctgctgaaaa atcccgaatt gttgtcccgc gcgatgtatt ccgcagtggt ctcaaacaat
     2581 attgccggta tccgcgttat tttgccgatt tacctacaac aggcgcagca ggataagatg
     2641 ttggcacttt atgcacaagg gattttggcg caggcagacg gtagggtgaa ggaggcgatt
     2701 tcccattacc gggaattgat tgccgcccaa cccgacgcgc ccgccgtccg tatgcgtttg
     2761 gcggcagcat tgtttgaaga caggcagaac gaggcggcgg cagaccagtt cgaccgcctg
     2821 aaggcggaaa acctgccgcc gcagctgatg gagcaggtcg agctgtaccg caaggcattg
     2881 cgcgaacgcg atgcgtggaa ggtaaacagc ggcttcagcg ttacccgcga acacaatatc
     2941 aaccaagccc cgaaacggca gcagtacggc aaatggactt tcccgaaaca ggtggacggc
     3001 acggcggtca attaccggct cggcgcggag aaaaaatggt cgctgaaaaa cggctggtac
     3061 acgacggcgg gcggcgacgt gtccggcagg gtttatccgg ggaataagaa attcaacgat
     3121 atgacggcag gcgtttccgg cggcatcggt tttgccgacc ggcgcaaaga tgccgggctg
     3181 gcagtgttcc acgaacgccg cacctacggc aacgacgctt attcttacac caacggcgca
     3241 cgcctttatt tcaaccgttg gcaaaccccg aaatggcaaa cgttgtcttc ggcggagtgg
     3301 gggcgtttga agaatacgcg ccgggcgcgt tccgacaata cccatttgca aatttccaat
     3361 tcgctggtgt tttaccggaa tgcgcgccaa tattggatgg gcggtttgga tttttaccgc
     3421 gagcgcaacc ccgccgaccg gggcgacaat ttcaaccgtt acggcctgcg ctttgcctgg
     3481 gggcaggaat ggggcggcag cggcctgtct tcgctgttgc gcctcggcgc ggcgaaacgg
     3541 cattatgaaa aacccggctt tttcagcggt tttaaagggg aaaggcgcag ggataaagaa
     3601 ttgaacacat ccttgagcct ttggcaccgg gcattgcatt tcaaaggcat cacgccgcgc
     3661 ctgacgttgt cgcaccgcga aacgcggagt aacgatgtgt tcaacgaata cgagaaaaat
     3721 cgggcgtttg tcgagtttaa taaaacgttc tgattgctgt tccttttcgg aggaaaccct
     3781 gccggcggcg gtatcacggc gggcatcggc ggctttcggg cggtgctttg cgtgccgccg
     3841 cgtgtgcgga aacgcattcc ggtttttccg gcataacggc gatgcgaggt aaaatgccgt
     3901 ctgaaacccg attcgggctt cagacggcat tgtcgcggtt gcggcaggcg ggttcaccag
     3961 attccgtcaa aggttttcgc gccgcgccaa aatttccacc tgtcggcggg tttgaaggtc
     4021 agcgtaccgc cgtgttgtcc gtccgtggtg atgtccagcc gtttgatttt gccgatgcgg
     4081 acggcttcgt aaagcggtgc gaaccagcgt tcttcccact gctgcaatat tgccgcatac
     4141 cgctccctgt cccctgtcag ggcggtcagg cgcaaatcgt ccataaacag gatatggtgc
     4201 gtgtcgggta ggtatgccgc cgtttcttca taggcgcgga agttgtcggg caatgcgcgg
     4261 cggtcggggt ggaaacggct ccaaaccgta tcggcgaaaa gcgtgccgcc ttgactgctt
     4321 ccgtgcagac tgtcccaaag ccacagcccg ttcaactcgg gcagcccgcg tttcttgcgg
     4381 ttatggttga cggggtgcgc cgccagccac atttggattt cggtttggac gcgcagccat
     4441 tccaacgcgt cttccccgtc cggctgatcg tccgcgccca acaatccgcc caagtccaaa
     4501 acgggcttcg cgccccagcg gtacgcgcaa ggaagggaaa ccagccataa ttcgggcagg
     4561 acgggaacga aacgccatgg aatgtcgccg taaaacgccg acaggtcgcg gcagagccgt
     4621 tccgcttcat ccgtaccgac gttcagatat tccgccgtta gcacatttgc ctgatgcatc
     4681 cccatctttt gccagacggg cgtggcgagc gcgacggctt cagacggcat attcaggctt
     4741 tgtgccgcgc gttccgccag tctgccgcac cacaaataac gcgcgtaaaa tgccgaagcc
     4801 gtgcagcttt ggcggtgcag cgagccgtat tgcaggattt tgttgaaggc gggcaggcag
     4861 agaggtattc gggtttcgtc ttcatccaaa ttgagcgagg gaatggcgag ggtgagtttc
     4921 atcgtttgac gtttcagaaa tgcaggtcag gcgcaacatt atagaggatt cggcgcaaac
     4981 gccgtcaaaa aggaacaata tggctgtctt cccactttcg gcaaaacatc ggaaatacgc
     5041 gctgcgcgcg cttgccgttt cgattatttt ggtgtcggcg gcatacattg cttcgacaga
     5101 gaggacggag cgcgtcagac cgcagcgcgt ggaacaaaaa ctgccgccgc tgtcttgggg
     5161 cggcagcggt gttcagacgg catattgggt gcaggaggcg gtgcagccag gcgactcgct
     5221 ggcggacgtg ctggcgcgtt cgggtatggc gcgggacgaa attgcccgaa taacggaaaa
     5281 atatggcggc gaagccgatt tgcggcattt gcgtgccgac cagtcggttc atgttttggt
     5341 cggcggcgac ggcggcgcgc gcgaagtgca gttttttacc gacgaagacg gcgagcgcaa
     5401 tctggtcgct ttggaaaaaa aaggcggcat atggcggcgg tcggcttctg aggcggatat
     5461 gaaggttttg ccgacgctgc gttcggtcgt ggtcaaaacg tcggcgcgcg gttcgctggc
     5521 gcgggcggaa gtgcccgtcg aaatccgcga atccttaagc gggattttcg ccggccgctt
     5581 cagccttgac ggtttgaagg aaggcgatgc cgtgcgcctg atttacgaca gcctgtattt
     5641 ccacgggcag caggtggcgg cgggcgatat tttggcggct gaagtcgtta agggcggcac
     5701 aaggcatcag gcgttctatt accgttcgga caaggaaggc ggagggggcg gcaattatta
     5761 cgatgaagac ggcagggtgt tgcaggaaaa aggcggcttc aacatcgagc cgctggtcta
     5821 tacgcgcatt tcttcgccgt tcggctaccg catgcacccc atcctgcaca cttggcggct
     5881 gcacacgggc atcgattatg ccgcaccgca gggaacgccg gtcagggctt ccgccgacgg
     5941 cgtgattacc tttaaaggcc ggaagggcgg atacggcaac gcggtgatga tacgccacgc
     6001 caacggtgtg gaaacgctgt acgcgcactt gagcgcgttt tcgcaggcgc aaggcaatgt
     6061 gcgcggcggc gaggtcatcg gttttgtcgg ttcgacaggg cgttcgaccg ggccgcacct
     6121 gcattacgag gcacgcatca acgggcagcc cgtcaatcct gtttcagtcg cattgccgac
     6181 acccgaattg acgcaggcgg acaaggcggc gtttgccgcg cagaaacaga aggcggacgc
     6241 gctgcttgcg cgcttgcgcg gcataccggt taccgtgtcg caatcggatt gaagtttgaa
     6301 ccggcgacga aaacaatgcc gtctgaaaat ctgcaaacag gttttcagac ggcatttata
     6361 gtggattaac aaaaaccagt acgtcgttgc ctcgccttag ctcaaagaga acgattctct
     6421 aagatgctga agcaccaagt gaatcggttc cgtactattt gtactgtctg cggcttcgtt
     6481 gccttgtcct gatttttgtt aatccaccat acagtcgatt aaaataaaac taagccaagg
     6541 aagcactgcc gtcattcccg tacgggcggg aatccggaca ccacggtacg gaaacccatc
     6601 cgccgtcatt cccgcgaaag cgggaatcta gaaatgcaac gcggcaggag tttatcggaa
     6661 atgatggaaa cccaacgcac cggattccca ctttcgcggg aatgacgaag taggtaggaa
     6721 tccggattta tccgttccga cagtgtttgc aaataaaaga aaacccaacc gtcccgattc
     6781 ccggcagggc tgttttacgg attttgcagc gagggcgcgg ggcggtcttg cgcctgtttg
     6841 gtttgcaggg tcgtcagttt tttcgtcagc agattcagaa tcacgccgta ggcgggcagg
     6901 aaaaacagac cgcagacggt gagtttgaac aggtaatcga caaaagcgat gccctgccag
     6961 tttgccgcca taaattcatc gctgcttgca tagaaggcaa cggcgaaaaa taccaacgta
     7021 tccaaggcgt tgccgatgac ggttgatgcg gtcggtgcaa tccaccacgc tttcagacga
     7081 cgtaatttgt tgaacacaaa aatatcaagg atttgtccga gcgcgtaggc ggcaaagctg
     7141 gccaaggcga tgcgtccgac aaaggtgttg aattcggata gtgcgcccaa gcctgtccaa
     7201 ctgccgttgt ggaacaaaac ggaaaagacg taggaaagca aaagggcggg gaacatcacc
     7261 caaaagataa tccgccgtgc caagtgcgaa ccgaaaatgc ggacggtcag gtcggtggcg
     7321 aggaagatga aggggaagga aaacgcaccc caagtggtgt ggatgccgaa aatttggaag
     7381 gggaactgca ccagatagtt gctggcggcg atgatgagga tatggaaaag caccagccag
     7441 aagagtgcct tctgttgctg tgcggcagta aatgcgtaca taaaaatctt tcggaaaggc
     7501 gttcagacgg cataccgtat cgaaggaatg ccgtctgaaa tatgggaagg atggtttatt
     7561 gtgcgtcgtg ctcaaacagg cgtttacgcg ccaatgcttc aaactcggtg cctgctttgc
     7621 cgtaattggc gaaaggatga atggcgatgc cgccgcgcgg tgtgaactcg ccgaatactt
     7681 cgatgtattt cggatccatc agggcaatga ggtctttcat gatgatgttg acgcagtctt
     7741 catgaaaatc gccgtggttg cggaagctga agaggtagag tttcagggat ttgctttcca
     7801 ccattttgat gtgcggaatg tagcggatgt agatggtggc gaagtcgggc tgcccggtca
     7861 tggggcagag gctggtgaac tctgggcaga cgaatttgac gaaatagtcg ttgtcgggat
     7921 gtttgttgtc gaatgcttcg agaatttcag gcgcgtagcc ggtcggatat cgggttttct
     7981 gattacccaa aagcgagatg ccttgcagct cttcggtatt gcgggacata gggtttcctt
     8041 agttttttag tgtgggaggt tttcgaacca cggggcggcg attgtaatat aagcggcggt
     8101 atctgtgtag ttttcttcag acggcatggt ttggacggcg gcgttttccg t
//
LOCUS       DAWFLT010000085         8132 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWFLT010000085 DAWFLT010000000
VERSION     DAWFLT010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.085"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(67..291)
                     /locus_tag="WGR74_001803"
     CDS             complement(67..291)
                     /locus_tag="WGR74_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ0906169.1"
                     /translation="MDAVQELERRIVELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(292..1680)
                     /locus_tag="WGR74_001804"
     CDS             complement(292..1680)
                     /locus_tag="WGR74_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ0906170.1"
                     /translation="MNAVVVAVIVMLSLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAVAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(1766..2587)
                     /gene="prmC"
                     /locus_tag="WGR74_001805"
     CDS             complement(1766..2587)
                     /gene="prmC"
                     /locus_tag="WGR74_001805"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0906171.1"
                     /translation="MTFDEWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(2577..3038)
                     /locus_tag="WGR74_001806"
     CDS             complement(2577..3038)
                     /locus_tag="WGR74_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906172.1"
                     /translation="MQEQNRKSSFPIVMLLVSVALWIASLSNVAFYLGNHGSMEGLTV
                     LILGSIFASLDIRYCAVYANYVWLAAIVLLALRKKVVPVHAALWGLALVAFSVKAVYV
                     DEAGNTSDIVRYGAGFYLWYAAFAVATIGTFAGKNKERKAASAADGIKNDV"
     gene            complement(3103..4587)
                     /gene="tldD"
                     /locus_tag="WGR74_001807"
     CDS             complement(3103..4587)
                     /gene="tldD"
                     /locus_tag="WGR74_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ0906173.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVHRFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILTGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      4765..4868
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4934..6157
                     /gene="cytX"
                     /locus_tag="WGR74_001808"
     CDS             4934..6157
                     /gene="cytX"
                     /locus_tag="WGR74_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ0906174.1"
                     /translation="MSGNASSPSSSSAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRQARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGVSANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            6154..7254
                     /locus_tag="WGR74_001809"
     CDS             6154..7254
                     /locus_tag="WGR74_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ0906175.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWHADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECVPEDLQAQYDWLIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHIFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYNRARRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            7275..7892
                     /gene="thiE"
                     /locus_tag="WGR74_001810"
     CDS             7275..7892
                     /gene="thiE"
                     /locus_tag="WGR74_001810"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ0906176.1"
                     /translation="MTFLPLKSPLKFYAVVPTADWVERMVEAGADTVQLRCKALHSDE
                     LKREIARCAAACQGSHTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYIASGAIFPTTTKQMPTAPQGLDKLREYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT         1749 a         2505 c         2189 g         1689 t
ORIGIN      
        1 ttaggtttgc atcggcggaa tattcaaaca cagccttttt taaggaaatc cggatacggc
       61 ggcgcatcaa taatgcggcg gaatctcgtc gcgcagggaa tacggctctt gcgcgtcggg
      121 attcctgtcc tgcatttttt gatacagcag cctcaactga gcctgctgca aatccagcgt
      181 ctgccgcaat tccgccacca tcgcgttcag gccggcgatt acgtcctcct gaagcgcgga
      241 ttggatttcc agttcgacaa tacggcgttc caactcttga accgcgtcca tttacagcac
      301 catcgcggca atccagccgg caatcagcag cgggatgttg tagtggatga aggtcgggat
      361 aacggaatcg cggatgtggt cgtgctgccc gtcggcgttc agccccatcg tcgggcccag
      421 cgtggaatcg gacgcaggcg aaccggcatc gcccaacgcc cccgccgtgc cgacaatggc
      481 gacggtggca agcggcgaaa aacccaaacc gacacacaaa ggcacataaa tcgcggcaat
      541 aatcggcaaa gtggaaaagg acgaaccgat gcccatcgtt accaaaagcc ccaccaccag
      601 catcgccaat gccgccatac ctttgctgtt gccgaatatc gccatactgc tttccaccag
      661 cggctgaata tgcccggtcg cattcatcac ggcggcaaaa ccctgcgcgg caatcataat
      721 gaagccgacc atcgccatca tcttgatacc ttcgccgaat acgtcgtttg ccttgtcgcg
      781 gttaatgacc cccaacatca taaatacggc gaaaccgagc atcgcgccca acaccagcga
      841 gtcttcatac atcaactgga tggcaaagca tacggcaatg gcgacggcgg cggccaggct
      901 gcggtaggcg gacggctgcg gacggtttgc cgcatcggcg ttgcccgccg tatcggcatt
      961 gttgctttgg tacaggcgcg gtttgcggta atggacaaac gccagcagga gtccggccag
     1021 cattcccaac gcgggaatcg ccattgccgc catcacgtta atgtttttca catcaagctg
     1081 cggcgcggcg gaatggatgt tgcccaacag gatttcgttc aaaaaaatcg cgccgaagcc
     1141 gtaaggcagg aacatataag tcgtaaccag cccgaaagtg atgacgcacg caatcaggcg
     1201 gcggtcgatt ttcaggcggt tgaacaccaa aagcagcggc ggaacaatca tcgggataaa
     1261 ggcaatgtgg atggggatga tgttctgact cataatgccc atcacaagga tgatggaaag
     1321 cagcagccat ttgaccgcgc cctcgcccga acgcacgctg tcgggcatac cgccccggtt
     1381 cagtttgcgg acgaccgcgc cggcaagctg ctgcggcagg ccggaatggg taatcgccat
     1441 cgcaaacgca ccgagcatcg cataagaaag cgcaatcttc gcgccgcctt ccaaaccttt
     1501 gttgaacacg gggataatcc ccgcctgact gacctgtccc gccgcatcgg caatgttttg
     1561 cagcggcata cccgccaccg cgccgccgac aaacgcgccg accgtcaggc tcaataccac
     1621 gtgcacgcgc gacagcgaca gcgacagcat aacgattacg gcaactacga ctgcattcat
     1681 tgtttatcgc tccaaaccta taaatgttta catatcgaaa cacatcataa cccaataacg
     1741 gaaaacccgc caattttgca aacaattatt tcaaatgctt catatacttc cccagcgtaa
     1801 ccctgtccaa acccgccaag tccggcaggg tttccactcc cgaaaaacca ttctccgcca
     1861 acacgccgcg caccgccgcg ccctgatcga aaccgtgttc cagcaataaa aaaccgcctt
     1921 ccgccaaacg gtcgggcgcg ccttgcgcca aggtgcggat gcagcttagg ccgtctgaaa
     1981 agtcggtcag cgcgatttgc ggctcaaacc gcaaatcgcc ttgcaacaaa tgtttatcgc
     2041 cgttttcgat atagggcggg ttggacacga tgatgtccca tttcccttca gacggcatat
     2101 cggtgtcgaa ccacgaaccg tgtgcaaatt cgacccgcgc gcccaaatct gccgcatttt
     2161 tccgcgccgt ttcaagggcg ggcgtgctga tgtcggatgc gcgcacaaac gcatcggggc
     2221 gttcgagtgc gacggttacg gcaaccgcgc cgctgcccgt ccccaaatcc cacacgcgcc
     2281 cgttttcggg caggcgcgcc aatacggctt cgaccaagtg ttcggtttcg gggcgcggaa
     2341 tcagcacgct cggattgact gtaaagcgtc tgccataaaa ttcgcgcaca cctaaaatat
     2401 aggcaaccgg ctcgccgttc agacggcgtt gcgccagcct gtccgcccgc tgtcggactt
     2461 cgtccggcat ttcttccccg ccccgcgtca acaactgcac gcgcgtatat tccgaaacat
     2521 attgcagcag cattcttgct tcaattttag gcagttttga caagcccaac cattcgtcaa
     2581 acgtcatttt ttatcccgtc tgccgccgat gcggcttttc tttccttatt ctttccggca
     2641 aacgtaccga tggtggcaac cgcaaatgcg gcataccaca aataaaatcc tgcaccgtag
     2701 cgcacaatat ccgatgtatt ccctgcttca tcgacgtata cggctttcac actgaaagcc
     2761 accaacgcca agccccaaag tgccgcatgg acaggcacga ccttcttccg caacgccagc
     2821 aaaacaatgg ccgccaacca aacataattc gcatagaccg cacaatacct gatatccaaa
     2881 gaagcaaata tcgaccccaa aatcaaaacg gtcaaaccct ccatgcttcc atgattgccc
     2941 aaataaaatg caacattgga taaagacgct atccacaggg caaccgacac cagcaacatc
     3001 actatgggaa aacttgattt ccgattctgt tcctgcatgg ttttatccta atgtaaaagg
     3061 ccgcctgaaa acctttcaga cggcatcgtg ccggattccg cgtcagattg cgctgccgcc
     3121 gacggtcagt ccggcatcaa tccgcaaagt cggttgcccc acgccgacgg ggacgctctg
     3181 cccttctttg ccgcacacgc cgacaccgct gtcgagcgcg gtatcgttgc ctatcatcga
     3241 aacgtgtttc agcacttcgg gaccgttgcc gataatggtc gcgcctttga cgggatattg
     3301 cagcctgccg ccttccaccc accatgcttc ggacgcgctg aacacaaatt tgccgctggt
     3361 aatgtccacc tgtccgccgc cgaagttgac ggcgtaaatg cctttgtcga tggacgcgat
     3421 gatttcttcc ggctcatagc tgccgttttc cataaaggta ttggtcatgc gcggcatagg
     3481 ggcggaggcg tagctttcgc ggcggccgtt gccggtcgat tgcgtacccg tcaggcgggc
     3541 gttggtttcg tcctgcatat agccggtcaa aataccgtct tcaatcaata cggtgcggcg
     3601 ggtttcgttg ccttcgtcgt cgatgttgag cgaaccgcgc cggccggcaa tatcgccctg
     3661 atcgacgacg gtaacgcctt tggcggcgac gcgctcgcct attctgccgg aaaagacgct
     3721 ggtccccttg cggttgaaat cgccttccaa accgtgtccg accgcttcgt gcagcaacac
     3781 gcccggccag ccgttgccca aaacgacggt catttcgccg gcgggcgcgg ggcgggattc
     3841 gaggttggtg agtgcctgtt tgacggcggc atcgacaaac cgatgaacca agttttcatc
     3901 gaaataagcc aagtcgtagc gtccgccgcc gcccgcgctg ccctgttcgc ggcgttcgcc
     3961 ctgtttggcg ataacggtaa cgttcaggcg caccatcggg cggatgtcgg cggcgtgttt
     4021 gccgtccaga cgggcgaggt aaaccatatc gtattcgcag gtcaaaccgg ccatcacttg
     4081 cacgatgcgc ggatcggcgg ctttggcgat tgcttccact ttgttcaaca gcgcgacttt
     4141 ggcggcggaa tcgaggccgg caatggggtc ggacgcggaa caaaccggct tgccgcgcgt
     4201 ttcagacggc attttggcgg acaccttgcc gcctgccgcc ccaatcgcgc ggacggcgcg
     4261 ggcggaacgg tttatcgaat cgatgcacag gctgtcggcg taggcaaagg cggttttgtc
     4321 gcccgaaacg gcgcgcacgc ccacgccctg attgatttgg aagctgcccg atttgacgat
     4381 gccctcttcc aaatgccagc tttcataagc ggtgcgctgg cagtagatgt cggcgtaatc
     4441 gacgtggtgc gcgccgatga tgcacaggct tttggcgagc agttcggggg aaaggcggtt
     4501 ggcttcgagc agccgcgcct gtacggcgga ataggtcgga tgcatagtgt cggcgcataa
     4561 aaaatcaggg gcttgattat acggcatttg ttatatagtg gattaacaaa aaacagtacg
     4621 gtgttgcctc gccttgccgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat
     4681 ttttgttaat ccactataga aatgcgccgt gccgcctgaa atgtaagatt tttgccaacg
     4741 ccccctgctt ttgtgtacac ttaaagctcc ttgtcggagt gccgccgccg ggcggctgag
     4801 attgcgaaag cagaatccgt agaacctgtc ggggtaatgc ctgcgtagga aacaaaacct
     4861 ccccgccctt tcgggcttcc gttccctttt ccgcacttcc ccgccccatt ttcatgtttt
     4921 ttaaggactt gatatgtcgg gcaatgcctc ctctccttca tcttcctccg ccatcgggct
     4981 gatttggttc ggcgcggcgg tatcgattgc cgaaatcagc acgggtacgc tgcttgcgcc
     5041 tttgggctgg cagcgcggtc tggcggctct acttttgggt catgccgtcg gcggcgcgct
     5101 gttttttgcg gcggcgtata tcggcgcact gaccggacgc agctcgatgg aaagcgtgcg
     5161 cctgtcgttc ggcaaacgcg gttcagtgct gttttccgtg gcgaatatgc tgcaactggc
     5221 cggctggacg gcggtgatga tttacgccgg cgcaacggtc agctccgctt tgggcaaagt
     5281 gttgtgggac ggcgaatctt ttgtctggtg ggcattggca aacggcgcgc tgattgtgct
     5341 gtggctggtt ttcggcgcac gcaaaacagg cgggctgaaa accgtttcga tgctgctgat
     5401 gctgttggcg gttctgtggc tgagtgccga agtcttttcc acggcaggca gcaccgccgc
     5461 acaggtttca gacggcatga gcttcggaac ggcagtcgaa ttgtccgccg tcatgccgct
     5521 ttcctggctg ccgctggccg ccgactacac acgccaagca cgccgcccgt ttgcggcaac
     5581 cctgacggca acgctcgcct atacgctgac gggctgctgg atgtatgcct tgggtttggc
     5641 agcggcgttg ttcaccggag aaaccgacgt ggcaaaaatc ctgctgggcg caggtttggg
     5701 tgcggcaggc attttggcgg tcgtcctctc caccgttacc accacttttc tcgatgccta
     5761 ctccgccggc gtaagtgcga acaacatttc cgcgcgtttt gcggaaacac ccgtcgctgt
     5821 cggcgttacc ctgatcggca cggtgcttgc cgtcatgctg cccgttaccg aatatgaaaa
     5881 cttcctactg cttatcggct cggtatttgc gccgatggcg gcggttttga ttgccgactt
     5941 tttcgtcttg aaacggcgtg aggagattga aggctttgac tttgccggac tggttctgtg
     6001 gcttgcgggc ttcatcctct accgcttcct gctctcgtcc ggctgggaaa gcagcatcgg
     6061 tctgaccgcc cccgtaatgt ctgccgttgc cattgccacc gtatcggtac gccttttctt
     6121 taaaaaaacc caatctttac aaaggaaccc gtcatgaccc gtatcgccat cctcggcggc
     6181 ggcctctcgg gaaggctgac cgcactgcag cttgcagaac aaggttatca gattgcactt
     6241 ttcgataaag gctgccgccg gggcgaacac gccgccgcct atgttgccgc cgccatgctc
     6301 gcgcctgcgg cggaagcggt cgaagccacg cccgaagtgg tcaggctggg caggcagagc
     6361 atcccgcttt ggcgcggcat ccgatgccgt ctgaacacgc acacgatgat gcaggaaaac
     6421 ggcagcctga ttgtgtggca cgggcaggac aagccattat ccagcgagtt cgtccgccat
     6481 ctcaaacgcg gcggcgtagc ggatgacgaa atcgtccgtt ggcacgccga cgacatcgcc
     6541 gaacgcgaac cgcaactcgg cggacgtttt tcagacggca tctacctgcc gaccgaaggc
     6601 cagctcgacg ggcggcaaat attgtctgca cttgccgacg ctttggacga actgaacgtc
     6661 ccttgccatt gggaacacga atgcgtcccc gaagacttgc aagcccaata cgactggctg
     6721 atcgactgcc gcggctacgg cgcaaaaacc gcgtggaacc aatcccccga gcacaccagc
     6781 accctgcgcg gcatacgcgg cgaagtggcg cgggtttaca cacccgaaat cacgctcaac
     6841 cgccccgtgc gtctgctcca tccgcgttat ccgctctaca tcgccccgaa agaaaaccac
     6901 atcttcgtca tcggtgcgac ccaaatcgaa agcgaaagcc aagcccccgc cagcgtgcgt
     6961 tccgggctgg aactcttatc cgcactctat gccgtccacc ccgctttcgg cgaagccgac
     7021 atcctcgaaa tcgccaccgg cctgcgcccc acgctcaacc accacaaccc cgaaatccgt
     7081 tacaaccgcg cccgacgcct gattgaaatc aacggccttt tccgccacgg tttcatgatc
     7141 tcccccgccg taaccgccgc cgccgtcaga ttggcagtgg cactgtttga cggaaaagac
     7201 gcgcccgaac gcgatgaaga aagcggtttg gcgtatatcc gaagacaaga ttaaagccgt
     7261 ctgaaaggaa ccccatgacc ttcctgccac tcaaatcccc gctcaaattc tacgccgtcg
     7321 tccccaccgc cgattgggtc gagcgcatgg tcgaagcagg tgccgacacg gtgcaactgc
     7381 gctgcaaggc cctgcacagc gatgaattga aacgcgaaat cgcccgctgc gccgcagcct
     7441 gtcagggcag ccatacgcag cttttcatca atgaccactg gcgcgaggca atcgaagcag
     7501 gcgcgtacgg cgtgcatctc ggacaagaag acatggacac cgccgacctt gccgccatcg
     7561 ccgccgccgg tttgcgcttg ggtttgagta cgcactccgt tgccgaactc gaccgcgccc
     7621 tgtccgtaca ccctagctac atcgccagcg gcgcgatttt cccgaccacg accaagcaaa
     7681 tgcccaccgc cccgcaaggc ttggacaaac tgcgcgaata cgtcaaacag gcaggcggca
     7741 cgcccgtcgt cgccattggc ggcatcgatt tgaacaacgc ccgagccgta ctcgccaccg
     7801 gcgtttcctc actcgccgcc gtccgcgccg taaccgaagc ggcaaatccc gaagcggtgg
     7861 ttaaagcgtt tcaggctttg tgggatggat aaaaccgaaa gaagaaaatt caattgccgt
     7921 gtcggcaaaa cttagcccgt tatcgcaaat atgcttaact ttaaatgtgg catatcatca
     7981 aattccgtca ttcccgcgca agcgggaatc cgccttaaaa cttgagaaac catcatttga
     8041 aaaacagttt ccgaatttca aaaatggatt cccgcctgcg cgggaatgac ggcaaccggc
     8101 cggttgcgta tcaaaaaata aagtaattcg gc
//
LOCUS       DAWFLT010000086         8113 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWFLT010000086 DAWFLT010000000
VERSION     DAWFLT010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.086"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(214..942)
                     /gene="rmpM"
                     /locus_tag="WGR74_001811"
     CDS             complement(214..942)
                     /gene="rmpM"
                     /locus_tag="WGR74_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ0906177.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1306..2256
                     /locus_tag="WGR74_001812"
     CDS             1306..2256
                     /locus_tag="WGR74_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ0906178.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2369..3103)
                     /gene="fnr"
                     /locus_tag="WGR74_001813"
     CDS             complement(2369..3103)
                     /gene="fnr"
                     /locus_tag="WGR74_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ0906179.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3308..4729
                     /gene="hemN"
                     /locus_tag="WGR74_001814"
     CDS             3308..4729
                     /gene="hemN"
                     /locus_tag="WGR74_001814"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ0906180.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTVAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(5082..6656)
                     /locus_tag="WGR74_001815"
     CDS             complement(5082..6656)
                     /locus_tag="WGR74_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ0906181.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            6981..8081
                     /locus_tag="WGR74_001816"
     CDS             6981..8081
                     /locus_tag="WGR74_001816"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ0906182.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGADELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAELTQIFTVGDFRSRDLAAGGQGAPLVPAFHKALFRDDKE
                     TRVVLNIGGIANISVLPSDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVC
                     DAVSHAAADARQMYICGGGIRNPVLMADLAEYFGTRVSLRSTAELKLDPQWVEAAAFA
                     WLAACWINRIPGSSHKATGASKPCILGAGYYY"
BASE COUNT         2037 a         2262 c         2006 g         1808 t
ORIGIN      
        1 ggcaatggga cacaaacctg aatctgaatc tgaatctgaa cctgcacccc gattctgacg
       61 atagccgttt cagaaaacca cccaaaaaaa caaaccccgc aaaagcgggg gtttgtttca
      121 catcatcgat gccgaccgac gtttcacgaa tattgcaaca gtacctggta caaaatccac
      181 taacctcatg catcggcaag atattgccta gccttagtgt tggtgatgat tgtgtgccgg
      241 cacaacctga cgggttacga tgctgcggat tttcacatcc acacggcggt caggttcgat
      301 acatgcaatc agagcctcac gttttttggc tttagagact ttcgcaccca gtttggcaac
      361 ttcggcttca caaacttgag tcatttgcgc ttgagattcg cccaagccga cagcagaaat
      421 tctagaaaca ggtacgccgt tgctgaccag gttgtttgcc actacgtatg cgcggcgttc
      481 ggacagggcc tgattgtatt tgtcagaacc cataaagtcg gtatggcctt cgacgcggac
      541 agattggata ttggtttgac ccaggcgttg cgccaatact ttcaggttgt cttgagcttc
      601 ggcgcgcaat gaatccttat cgaaaccgaa cagggttttg gcagacaggg aaatggtttc
      661 atcaacatat tgcggagcct gctccacaac gacgacaggc gcgggtgcgg gttcgggttc
      721 tggctcgggt tcgggggcag caaccgcatc gccgcattct acgcgacctt ggcttgcttt
      781 atcaaagtag gcgtttttcc agcattcgcc atagttgttg cgtactattt cgttcgactg
      841 gccgcttacg gtgtaaccct gaacggacgc ctcgcccgca acagcagtgc cggaagcgag
      901 caatgcaacg aataatgcgc ttaatttcag ctgtttggtc attttattcc ctcattaaat
      961 ttgtacagca gatacagaag caactgctga attgccaaca tacaggccgc tgtagaaaca
     1021 tggcggcaaa tcggatattt cagtctccac tataaagaag cagggggagg actgtcaata
     1081 ctcaacgcct gaaaacaacc gtaaaacagc ctgtttcctg atagtctatc atgccgaaaa
     1141 atcaattccg tgtcattact ttagggggat tttttccata tcgcacgatt gccgttgcac
     1201 aaaaacaaca aacacaacgg caaagcccca taccgtaccg gcaagaaaat atgataaatt
     1261 ataaacaatg ttcagccacc cgacacagac ccacaccgac ccgccatgaa attacaacaa
     1321 ttgaaatacg ccttagaagt ttaccagcac aacctgaacg tttccgaagc ggccgaagcc
     1381 ttgtttactt cgcaacccgg catctccaaa caaatcaaat tgctggaaga agaaatcggc
     1441 attcagattt ttatccgcag cggcaagcgc gtggtttcgg tctcgcagcc gggcaaggtg
     1501 gttttggata ttgcggaacg tattttacgc gatgtacaga acattaaaaa tatcggcagc
     1561 gagtttaccg gacaggacag cggttcgctg acggttgcca cgacgcatac tcaggcacgc
     1621 tatgccctac ccttgattgt tgccgatttt gtgaaacgct atccgaaagt caacctgacc
     1681 atcaaacagg ggagccctgc cgccatcgca cgtatggtta cctcaggcga ggcggacttg
     1741 gcgattgtta cggaacggat agacgaccat cccgaactgg gaaaacttcc ctgctatgac
     1801 tggactcatg cggttatcgt accgaacgac caccccttgc tcgaatgcag aaaccccctc
     1861 cgcattgaag atttggcgag gtttccgctg attacttatg aatttgcatt caatgcgggc
     1921 agcagcatcg cgcgggcatt ttccaaagcc cgtttggaac agccggatgt cgcattggcg
     1981 gcggcggata cggacgtatt gaagacttat gtgcgcttgg gtttgggcgt gggactgatg
     2041 gcgaaaatgg cgtacaaccc ggatacggac ggcgatttgc agcttgtgga tgcggcacac
     2101 ctgttcgagc cgtcgccgac gtggattgct ttgcgcagcg atacttattt gcgcggatat
     2161 gcctacgact ttatccaagc gtttgcgccg cacctgacac gcgagaaggt ggataggatt
     2221 ctgtacacgc ccatcagcga ggatttttcg atttaggcgg ctgccggttt tcagacggca
     2281 ctttgcggca gatacaacaa acaggacaga tgttttcgtc tgccctgtgt ttattgagaa
     2341 tgctgtctga aatgttcgta cgggttaatc aaatggcgtg cgagcagccg gacaccattt
     2401 ttttcaacac ctgcagattg aggattttga tgtgcttatg ctcgacggaa atcaatcctt
     2461 cctgatggaa cttggataat gtgcggctga cggtttcaag tttcagcccg agataactgc
     2521 cgatttcttc gcgggacatt cttaagatga agtcgttggc agcaaaacct cgggaataaa
     2581 ggcgttggga aaggttcagc aggaaggcgg caatccgctc ttcggcgcgc atattgccca
     2641 acagcagcat aacaccttgg tcgcgcacga tttcacggct catcatgcgg aagaagtgcg
     2701 tacgcaggct ggggatgttt tgccccagtt cttcgatgtg ggtaaacggc agttcgcaca
     2761 cttcgctgtc ttccaaggcg accgcgtcgc aactgtgcac atgggaacag atgccgtcca
     2821 tgccgatgag ttcgcccgac ataaagaaac ccgttacctg atcgcggccg tcctgactgg
     2881 cgacggttgt tttgaagaag cccgaacgga tggcaaagag cgaggtaaag gcttcgccga
     2941 cacagaacag gtattcgccc tttttcaggc ggcggctttg acggatgacg gcatcgagtt
     3001 ggctgagctc gttgggcagc agcccgacag gcaggcagag ttcccgcaaa gaacaggaag
     3061 aacacagcgt tttcatctga tgtgtagtat tatgcgaagc cataccgtac ctttttgtgc
     3121 gctttgcccc atcatgatta tagtggatta aatttaaacc agtacggcgt tgcctcgcct
     3181 tgtccttatt taaatttaat ccactatatg tgcttattga cacatatcaa gacaggttta
     3241 tcatactgtg gcattctacc aaactatcca aaatttgact aacatcataa aaccgcacac
     3301 acacaccatg aaaatcattc agatacagaa caatcacaat gtaaacgatg accgccccga
     3361 gtttgaccgc gcgctgattg ccagcctgcc cgccagcggc ccgcgctaca cttcctaccc
     3421 taccgccgac cgtttccatg acggtttccg cgaaggcgaa tatatcaaag ctttacattt
     3481 gcgcggtatg ggcgcgttaa acaaaccgct ttccctttac attcacattc cgttctgcaa
     3541 caccatctgc tactactgcg gctgcaacaa aatcatcacc aaagacaaaa gccgcgccga
     3601 tgcctacatc gaatatcttg aaaaagaaat ggaactgctc gctccacatc tgaacggacg
     3661 gcaccagctt gcccaactgc acttcggcgg cggcacgccg acctttttga gcgacgaaca
     3721 gatcgaacgt gtcttccgca tgatacgcaa acatttcgag ttaatcccca ccggcgaata
     3781 ctccatcgaa atcgacccgc gcaaagtcag ccgcgacacc gtcctcatgc tcggcaggct
     3841 cggcttcaac cgcatgagca tcggcattca ggatttcgac cccaaagtgc aggcggcggt
     3901 caaccgcatc caaagttacg aagaaaccaa agaagtcatc gatgcggcgc gcgaagcggg
     3961 gttcaaatcc gtcagcgtcg atttgattta cggcctgccg caccagactt cggaaagcat
     4021 caaaaccacc atcgataccg ttttgtcgct cgatcccgac cgcctcgccc tttatcacta
     4081 cgcccacctg ccgcacgtgt tcaaaccgca acgccgcatc gataccgtcg ccgttcccga
     4141 cagcgaagag aagctcgata tgctgcaata ctgcgtccaa accctaaccg aacgcggcta
     4201 cgtcttcatc ggcatggacc atttcgccaa acctgacgac gaactctcca tcgccctcaa
     4261 agaaggcttc ctccagcgca acttccaagg ctattcgacc tacgcggatt gcgatttggt
     4321 cgccatcggc gtgtcgtcca tcggcaaaat cggcagcacc tattcccaaa acgaacgcga
     4381 catcgatgcc tactatgccg ccatcgacga aggcagactg cccatcatgc gcggttatca
     4441 gctcaatcag gacgacatcc tgcgccgcaa catcattcag gatttgatgt gccgtttcgc
     4501 gctcgactat cggatttacg aaagtatgtt cggcatcccg ttcgaccgct acttcaaaga
     4561 cgaactggcg gatttggaaa aactcgccgg tttgggattg gtgcgcctga acagccacgg
     4621 gctgaccgtt accccgaaag gacgcttcct catccgcaac atcgccatgg tcttcgatta
     4681 ccacctgcgc cataaagaaa ccaaggcgaa atattcgcaa acggtgtgat tgcgtctaac
     4741 gtacaaatgc cgtctgaaag gctttttcag acggcatttt gctgccggca ggataagtgt
     4801 tttcaagaac aggcggcggc atatcataac gttccgcacc tttgtgtccg accgttccga
     4861 aaccaagata tagtggatta acaaaaacca gtacggcgtt gcctcgcctt gccgtactat
     4921 ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatcacta taccaaacgg
     4981 catatcccga cagaacagat tgtgcataag gcacaagccc gcacattcca tcaacaaaat
     5041 gccgtctgaa cacgggttca gacggcatca gtattttaca atcagaatac tgcctgtaaa
     5101 accaagtagc agacaaccga caatacggcg gcggcaggca gggtaatgac ccacgccaaa
     5161 ccgatgggct tcatcagttt ccagttggca ttgcggttga ccagaccgat gccgagtacc
     5221 gcgccgacca agatatgcgt actggacacg ggcagcccca tcagcgacgc gcccatcacg
     5281 acggaggcgg cggacagttc ggcggtaaaa cccgaagcag gatgcatttc cgccaaactc
     5341 gtaccgacgg ttttaatcac ctctttaccg acaaaccaca aaccgacaat cagcgcgata
     5401 ccgaaagtca gcatcgcaat cggggggacg acattttgcg cggcaacgct gttggtacgc
     5461 aaaacatcca taatcgcggc aaacggaccg atggcgttgg cgatatcgtt cgcaccgtgg
     5521 ctgaatgcga agccgcaggc ggtaaagacc tgcatccatg aaaacatctg aaaggtcgat
     5581 ttgcccaagt ctttacgctt gaggcttttg gcaaacacaa acgtccccat ccacaccgcc
     5641 gcgcctatca taaagatggt caggaagctg ttgacgttgc tcatccccaa atgcaggttt
     5701 ttcaagccct tgaaaatcag catagcggaa atcatcatcg cgccgaacga agcgataaag
     5761 ggaatccaag aatgcagcgc tttgtaggaa tcgacattgt ttttacggtt gtcgaacgca
     5821 taaagaccgc ggtaatactc cgattgcagc tcttgcggat cgaattcggg ttcgtcgtaa
     5881 atttgcgcgt cgtgcgccat tttggtggcg tactcgactt tttcggcttc ggacaaaccc
     5941 tcgaaaaaca ggcggtgccg ttctttatag gccttttttt cctgcttgat gcccttgagc
     6001 gtgccttccg cccaagcgtt gtaatctaag acgtttttct tgacgcgcga aaacagaaaa
     6061 taggacaccg cgccgcccaa cacgggcgac aatacccaag aaacaccaat accgcccagc
     6121 ttgccccaac gtatcaaatc gcccgatgcg gcatcgttca ttaccgccat acataccgcg
     6181 ctgccgacaa tgccgccgat aatggaatgg gtggtagata ccggaagccc ttttttcgag
     6241 gcaaacaaca gccacaacgc cgccgccaaa agcgcggaca tcataataaa cacaaactgt
     6301 atgggttcga aatcaacacc cttcaaatcg acgatgcctt tgcgtatggt attggttacc
     6361 tcgccgcccg cgatgaccgc gccgctgacc tcaaataccg ccgcaatcag caaagcctgc
     6421 gggatggtca gcgtacccgc accgacactg gtgccgaaag aattggcaac atcgttgccg
     6481 ccgatgttga acgccataaa cacgccgaac atcgtggcaa taatgaataa ggcggcgtaa
     6541 ttgtaatggg tataacccaa gccccaataa ataaaatagc cgactgtacc taccagcatg
     6601 gcggcaaaga cggcgttgat ggttttaaca tttgcgctca tttggatttg agccatggca
     6661 atcttccttt aatgtggtgg aaatcatgag tgttgccgac actgtactgc tgcctccggt
     6721 cgaactgccg tttcgggata tggcagacgc atccttcctt ctgaaccgca tccgaatacc
     6781 cccaaacctg accgaatgca aaactgctgc cggaaacggt ttgtatgaca cttctactac
     6841 tggatgttgc gggcgcatta taatattttt ccatccgtct tgaaacattt atttacactt
     6901 tatttacact gcggcggcaa atcggtatac gagcgtcaat acacgttaaa atggcgtttt
     6961 gcaccagttt gggagtgatg atggaaacac agctttacat cggcatcatg tcgggaacca
     7021 gtatggatgg ggcggatgcc gtgctggtac ggatggacgg cggcaaatgg ctgggcgcgg
     7081 aagggcacgc ctttaccccc tatcccgaca ggctgcgccg ccaattgctg gatttgcagg
     7141 acacgggggc ggacgaactg caccgcagca ggattttgtc gcaagaactc agccgcctgt
     7201 acgcgcaaac cgccgccgaa ctgctgtgca gtcaaaacct cgcgccgtcc gacattaccg
     7261 ccctcggctg ccacgggcaa accgtccgac acgcgccgga acacggttac agcatacagc
     7321 ttgccgattt gccgctgctg gcggaactga ctcagatttt taccgtcggc gacttccgca
     7381 gccgcgacct tgcggccggc ggacaaggtg cgccgctcgt ccccgccttt cacaaagccc
     7441 tgttccgcga tgacaaagaa acacgcgtgg tactgaacat cggcgggatt gccaacatca
     7501 gcgtactccc ctccgacgca cccgccttcg gcttcgacac agggccgggc aatatgctga
     7561 tggacgcgtg gacgcaggca cactggcagc ttccttacga caaaaacggt gcaaaggcgg
     7621 cacaaggcaa catattgccg caactgctcg acaggctgct cgcccacccg tatttcgcac
     7681 aaccccaccc taaaagcacg gggcgcgaac tgtttgccct aaattggctc gaaacctacc
     7741 ttgacggcgg tgaaaaccga tacgacgtat tgcggacgct ttcccgtttt accgcgcaaa
     7801 ccgtttgcga cgccgtctca cacgcagcgg cagatgcccg tcaaatgtac atttgcggcg
     7861 gcggcatccg caatcctgtt ttaatggcgg atttggcgga atatttcggc acacgcgttt
     7921 ccttacgcag caccgccgaa ctgaagctcg atccgcaatg ggtggaagca gccgcatttg
     7981 cgtggttggc ggcgtgttgg attaatcgca ttcccggtag ttcgcacaaa gcaaccggcg
     8041 catccaaacc gtgtattctg ggcgcgggat attattattg agcgcaaatg ccgtctgaaa
     8101 gcccgttccg ccg
//
LOCUS       DAWFLT010000087         8090 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWFLT010000087 DAWFLT010000000
VERSION     DAWFLT010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.087"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(113..1831)
                     /gene="argS"
                     /locus_tag="WGR74_001817"
     CDS             complement(113..1831)
                     /gene="argS"
                     /locus_tag="WGR74_001817"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ0906183.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGIAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLAFMGNTVVRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERAAALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSNYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATAPTVLSEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAESASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            complement(1923..3131)
                     /gene="pncB"
                     /locus_tag="WGR74_001818"
     CDS             complement(1923..3131)
                     /gene="pncB"
                     /locus_tag="WGR74_001818"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ0906184.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIREDLEVELDALCQLRFTHDELDYLRSLRFIKSDFVDYLELFQLQRRFVEVGTD
                     DKGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            complement(3172..4026)
                     /locus_tag="WGR74_001819"
     CDS             complement(3172..4026)
                     /locus_tag="WGR74_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ0906185.1"
                     /translation="MLPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            complement(4271..5047)
                     /locus_tag="WGR74_001820"
     CDS             complement(4271..5047)
                     /locus_tag="WGR74_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ0906186.1"
                     /translation="MSNNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIRHLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVECKQPFVLSGGAGGQKDP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKAKMRVPCVFSTENITPPQSGAAC
                     STDAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIAGKK"
     gene            5264..5728
                     /locus_tag="WGR74_001821"
     CDS             5264..5728
                     /locus_tag="WGR74_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ0906187.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            5810..6538
                     /gene="rph"
                     /locus_tag="WGR74_001822"
     CDS             5810..6538
                     /gene="rph"
                     /locus_tag="WGR74_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ0906188.1"
                     /translation="MPDYIRTSRAADSLRDIKITPHFLPHTDGSCLIECGNSKVICTA
                     SVDENVPSFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALHIAVGKLISDGILTENPIRE
                     AVAAVSAGIVGGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEGAPFSLEELG
                     KLIGLAEKGIRELVRYQQNALSAA"
     gene            6739..7956
                     /locus_tag="WGR74_001823"
     CDS             6739..7956
                     /locus_tag="WGR74_001823"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ0906189.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLAAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAIADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
BASE COUNT         1802 a         2080 c         2334 g         1874 t
ORIGIN      
        1 cgtttgtttg tttcgcattt tgggaggggc ggcggatacg gtgtatcgcg ccgctttttt
       61 cagattcgga taaggatggc gaggctgttg tccgcaatcg ggcggtgcgg ttttacatta
      121 cgtccaacac atcgatgccc agcaaatcca agccttgttt cagcgtgtcg ccggtcagct
      181 tcgccagttg caggcggctg ttgcggcttg cgctttcggc tttgagtatc ggacaggctt
      241 cgtagaagcg gctgaacagg gtcgcaattt gatagaggta ggcggcgagg tagtgcggat
      301 acgccgtgtc cgccacgctt tgcagcacgt cttcgaattt cagcagctcg gcggcaagct
      361 gtttttccag cggttcgctc aaaacagtcg gtgcggttgc gtcccattca ccggctttgc
      421 ggaacacgct ttgcacgcgg gtgtaggcat attgcaggta gggggcggtg ttgccttcaa
      481 acgaaagcat agcgtcccag tcgaacacat aattgctggt gcggtttttg ctcaagtcgg
      541 cgtatttgac cgcgccgatg ccgacggttt tgccgatttt ggcggcttcg tccgcgccca
      601 attcgggatt tttctctttc accaaagcgg cggcgcgttc gacggcttcg gtcagcagat
      661 cgaccagttt tacagtgtcg ccgctgcgtg ttttgaacgg tttgccgtct ttgcccatca
      721 tcgtgccgaa gccgacaaat gccgcgccga cgttttccgg cagatagcct gctttgcggg
      781 aagtggtaaa gagttgctca aagtgcaggg cttggcggtg gtcgacgacg tacagcaggc
      841 ggtcggcatg aagcgtgccg acacggtagc gcaaacacgc caagtcggtg gaagcataaa
      901 ggaagccgcc gccttgtttt tgcacgataa acgcggcggg ttcgccttct ttgtttttga
      961 actcgtccaa gaatacgact ttcgcgccgt cgtcttcgac cgccagacct ttttgaacca
     1021 aatcatcgac cactggctgc aaatcgtcgt tgtacttcga ttcgcccgcc acgtcttcgg
     1081 ggcgcagctt caagcccagc gtgtcgtaaa cggcttgggc gtgcgagagc gaaatatcga
     1141 caaactgttt ccacaatgcc aaaacggttt catcgccgcc ttgcagcttc acaacgtatt
     1201 cgcgcgcggt gtcggcgaag gcaggatctt cgtcaaagcg cactttggcg gcgcggtaaa
     1261 actgctccaa atccgccagc tcaaacgcgg cattgtcttt ttgctgctcg accaaataag
     1321 cgaccaacat gccgaactgc gtaccccagt cgccgacgtg gttttgacgg acgacggtgt
     1381 tgcccataaa tgccaacacg cgcgaaatgc tgtcgccgat gatgctggaa cgcaggtgac
     1441 cgacatgcat ttccttcgcc agattgggcg aggaatagtc gatgacgacg gtttgcggtt
     1501 tgtcggtttt cgctatgccg aaacgcgcgt cgttcaaggc cgtctgaatg ttttgcgcga
     1561 gaaattcggg gcgcaggcgc aggttgataa aaccgggacc ggcgacttcc gcgctttcga
     1621 tgacggcgtt gcctgccaat gcgtcggcga ccttttgcgc cagttcgcgc gggttttgtt
     1681 tggctttttt cgccgcaccc atcacgccgt tgatttggaa atcgccgtgt tcggcatttt
     1741 tggtcggctg caaaacaacg gggctgccgg cgatgcctgc ggcggcaaag gcggcggcgg
     1801 cttcgtgttc gacggtttga tgtaggttca tgaagttcgg tctttcggtt aaattcaaat
     1861 caggcggtat tttaaaagat tttatgcggc agaaacagga attgtgcgct ttttctgccg
     1921 gtttacggcg tttcgggttc gggtacgtcg aacacttggc gcaggtaggc gaggaaggtg
     1981 ctgttgttgg tcatggtttt gcccggagag tcggacagct tggcgacgga ctgcccgttg
     2041 cattcgacca gtttcaagac gatattcaaa ggcgtatgcc ccatatcgtt ggtcaggttg
     2101 gtgccgatgc cgaagccggt tttgaagcgg tctttgaaat attggtgtaa tgcccaagag
     2161 cgttcgatgt ccagcccgtc ggagaaggtc agcattttgg tgcggctgtc gattttgagc
     2221 ttttgatagt gggcgtaggc tttgtcgccc caaacgtaag ggtcgccgct gtcgtggcgc
     2281 agcccgtcga aaagtttggc gaaatagagg tcgaaatcgc gcaggaaggc atccataccg
     2341 accacgtcgg tcagggcaac gcccaaatcg ccccggtatt cgtgcaccca gctttcgagc
     2401 gcggcctttt ggaaattccg caggcgtacg tcgagggcct ggaaggcttg gaggaactcg
     2461 tgcgccatgg tgccgatggg ggtgatgccg agttttttgg cgagaaagac attgctggtg
     2521 ccgcgcacga tgcttggggc ggcttcgagc agggtgcgga tgacgtgttc ctgccacgcg
     2581 agcttgtagc ggcggcgcgt gccgaagtcg gaaatcagga agggcggttc gtcggggttt
     2641 tgtgcggcgg cgatttcttt gaggcgcgcg gctttggctt gaagccggcg ttcgccttct
     2701 tctatgactg caggggtttc caggcggcgg aagtaaagtt cgttgacaat ggcgaggatg
     2761 aagatttcaa aaaacatcgc ctgtatcatc ggaccttcga tgcggatgtt cagacggcct
     2821 ttatcgtctg tgccgacttc gacaaagcgg cgttggagct ggaagagttc gagataatcg
     2881 acaaagtcgc ttttaatgaa acgcaggcta cggaggtagt cgagttcgtc gtgggtgaag
     2941 cgtagttggc agagcgcgtc gagttcgact tccaagtctt ccctgatgtc ggcaagcgga
     3001 tagacggtcg aggcgttgcg gcagcggaat tcgtaaaggc tgtgcgtctg cggaaactgg
     3061 tgcagaacca cttgcagcat agtgaatttg tagaggtcgg tgtcaagcag cgaatgtatg
     3121 atgccggtca tatggcgtgt ctcccgattt ttggtggcgg cttggggcgt attagaacac
     3181 atcgcgcccg ccccgtgtgc cggaaatgcc gtctgaatgc gccccctcat gattgaggct
     3241 gattcggatt tctccgaaac cgacttcctg tacgattccg accaaacctt ctttggcaag
     3301 ctccaacagt gcgatgaagt tgacgaccac gtaagccgcg ccctgttcgg gattgaacag
     3361 gtcgtgaaac ctgcatatcc cgtgtccgtt caaacggcgc aggattgccg tcatttgcgc
     3421 gcgcacggag atggtttctt tgattacttc gtggctgcgc gtgtgttttg cccgagacaa
     3481 aatacccagc cacgcttgcg tcaagtcggt aatatagact tcgggcagct tggcttcgac
     3541 ggcaatttcc agcggcaggt aagcccacgc gaaatcccgt ccggctcggg gcagcgcgtc
     3601 caaaccctgc gccgccagct tcatctgttc gtaagccagc aggcggcgca ccaactcggc
     3661 acgcgggtcg gcttcttcgt cttcgacggt ttcggtacgc ggcagcagca ggcgcgattt
     3721 gatttcaatc agcattgccg ccatcaaaag atattccgcc gccaaatcaa actgataggt
     3781 ttctatttgg gcgatgtagt gcagatactg ctcggtaatc tttaccatcg gaatatccag
     3841 tacgtcgata ttctgtttgc ggatcagata cagcagtaga tccaaagggc cttgaaaact
     3901 gcccaatacg actttcagcg cgtcgggcgg gataaacaaa tcctgcggca aatcggtaac
     3961 gggttgaccg aatacccatg cgacagtgtg ctcggatggt gcagatgggg cagggatggg
     4021 cagcatggcg tgaatcggta agtggatttt aatataagtt tacaaaagaa aacaatctgc
     4081 cggcattcac gcataatgtg gtcgggcaga tgttcagacg gcattataac ggcttttaaa
     4141 tgcaaatgtt ttgaaaagga gtagattgtg aatacggttt taggcgtgcc gatgttggtt
     4201 tttctgaacc gacagtttga ttgccgcgtt tgttcaaatg ccgtctgaat cctgtttcag
     4261 acggcattgc ttattttttg cccgcgatgt gttccaccgc cgcctgtgcg caatatagcc
     4321 cgaacgaagc ggtaacgagc atgcttgcac cgtagccggc gcacgacaag ccttgcggag
     4381 cggcatcggt agaacacgcc gcgcccgatt gcggcggcgt gatgttttcg gttgagaaca
     4441 cgcacggcac gcgcattttg gctttcgtat cgcggctgaa gccgtagcgt ttccgcaagg
     4501 tgtagcgcag gttggcaagc agcgggtcgt gggttacgcg gctcaaatcg gcggtttgga
     4561 tcaacgccgg atctttttgt ccgcccgcgc cgccgctgag gacaaacggt tgtttgcatt
     4621 ccacaaaata agccgccatt gccgctttga cgcgcacttg gtcgatcgcg tcgatgacga
     4681 aatcaaaacc ttttccgaag tattccggca aattgtcttc ggtaacgaaa tcttcaattt
     4741 caaatacttc gcattgcgga ttgatttgtg taatgcgttc gcgcaaggcg gtaacttttg
     4801 ctttgccgaa gtcgccggtc agggcgtgca gctggcggtt gacattcgat tcggcaacgt
     4861 tgtccaaatc aatcaaagtc aaatgcctga tgcccgtccg cgccaaagcc tcgaccgccc
     4921 acgagccgac accgcccacg ccgactacgc agacgtgtgc ctgtgaaaag tgcgccaagg
     4981 cggagtctcc gtagagtctg gcgatgccgc cgaatcgtcg cgaagaggtc aaagcgttgt
     5041 tggacatggt cgaatcctgc aagattgcgt caaatcaaaa agtgaacgaa agccggcaaa
     5101 aaatgcctga ctgcacggaa gcggcatatt atactggaaa gcggtcgggc tgttcggctg
     5161 ttgtataaag cccgacggcg ggtctataat gaaacagcgc aaactgtccc tgcgcccatc
     5221 cgacaagagg gcaccgcgag gcggtgcaaa aggagaatac gatatgtaca aacatttggt
     5281 tgttgccgtt gacggcagtg aaacgtccat caatgccctg aaacatgccg ccgagcttgc
     5341 cggggtcaac ggtgcgcgtc tgactttggt acacgttgcc aatcctgccg aatatatggc
     5401 gctcgccccc gaatttttgc aacacgaaag ctacgaagcc gccgccgtcg cgcaaggcaa
     5461 cgaagttttg gatgccgccg agcgcaccgc ccgggaactg ggtgtggaaa ataccgtcaa
     5521 acacctgctg gttgccaata agggtgcgcg tgaaatggcg caggatttgg ttgattatgc
     5581 cgatgaaaac ggtgccgacc tgctggtgtt gggtacgcac ggacgtaccg ggctgatgca
     5641 ccttttaatg ggcagttttg ctgaaacggt aatgcgccaa agccacctgc cgcttttgat
     5701 tatccgcagc aaagccgaag aggcgtagaa attttcagtt tgaaggcctt aaatgtgatt
     5761 tttcatattt aaggcttttt aatttatctg tttttaacgg gagggcagta tgcctgatta
     5821 catccgcacc tcacgcgccg ccgacagcct gcgcgatatc aaaatcaccc ctcatttcct
     5881 gccgcatacg gacggttcgt gccttattga atgcggcaat agcaaagtga tttgtaccgc
     5941 ttccgtggat gaaaatgtcc catcgtttct gcgcggtaaa aaccaaggct gggtaacggc
     6001 ggaatacggg atgctgcctg cgtccaccgc ttcccggatg cgccgcgaag cttcggcggg
     6061 caaacagtcc gggcgcacac aagaaatcca acgccttatc gggcgttcgc tgcgcgcggt
     6121 cgtggatatg gaaaaactcg gcgaacgaca aatcttgatt gactgcgatg tgattcaggc
     6181 ggatggcggt acgcgcacgg cttcgattac cggcgcattt gtggcactgc acattgccgt
     6241 cggaaagctg atttcagacg gcatattgac cgaaaatccc atccgcgagg ccgttgccgc
     6301 cgtatccgca ggcatagtcg gcggagtgcc gcttttagat ttggattatc ctgaagattc
     6361 cggttgcgac agtgatgtaa atatcgttat gaccgcatcg gggaaaatca tcgaaatcca
     6421 aggtacggcg gaaggcgcgc cattcagttt ggaagaattg ggcaagctga tcggactggc
     6481 ggaaaaaggc ataagagagc tggtgcgtta tcagcaaaat gcgttatccg ccgcctaatt
     6541 actgtccgta tgccgtctga aaaccgttca gacggcattt tgtcaaaaat ctgaaagaac
     6601 aggtttttcg ctgaccgctg tcaaacggca ggcgggaaag cgcagtccgt tcttgaactt
     6661 catgtaaaaa atagtaataa tagccgcttt tccagcaaat atgctgcccg accaacccta
     6721 cacaagaaag cacgaaacat ggcgttaatc gtacataaat acggcggcac atccgtaggc
     6781 tcgcccgaac gcattaaaaa cgtggccaaa cgtgtcgcca aagcccgcgc cgaaggacac
     6841 gacatcgtcg tcgtcgtatc cgccatgagc ggtgaaacca accgcctggt cgcgttggcg
     6901 cacgaaatgc aggagcaccc cgatccgcgc gaattggacg tggttttgtc caccggcgaa
     6961 caagtaacca tcggcctttt ggcgatggca ttgaaagaca tcggcgtgga tgccaaaagc
     7021 tacacaggct ggcaggtcgc cctcaaaacc gataccgccc acaccaaagc ccgcatcgaa
     7081 agcattgatg acgaaaaaat gcgtgccgac cttgccgccg gcaaagtcgt catcgtcgcc
     7141 ggcttccaag gcatcagcag tgaaggcgat atttccacgc tcggacgcgg cggttccgac
     7201 acttccgccg ttgcgcttgc cgcagccctc aaagcggacg aatgccaaat ctataccgac
     7261 gtagacggcg tttacacaac cgacccccgc gtcgtacccg aagcgcgccg catggatacg
     7321 gttacatttg aagaaatgat cgaattggca agcctcggct cgaaagtctt gcaaatccgt
     7381 tcagtagaat tcgccggaaa gtacaaagtg cgcctgcgcg tactgagcag cctgcaagac
     7441 ggcggcaacg gcaccttaat tacctttgaa gaggacgaca acatggaaag agctgccgta
     7501 accggtatcg cattcgataa aaaccaagcc cgcatcaacg tgcgcggcgt acccgacaaa
     7561 cccggcgttg cctatcagat tttaggcgcg atcgccgatg ccaacatcga agtcgatatg
     7621 attatccaaa atgtcggcag cgaaggcaca accgattttt cctttaccgt accgcgcggc
     7681 gattacaagc agactttgga aatcctgtcg gaacgccaag acagtatcgg cgcggcttct
     7741 attgatggcg acgacaccgt atgcaaagtc tccgcagtcg gtttgggtat gcgttcgcac
     7801 gtcggtgtag ccgccaaaat cttccgcacg ctcgccgaag agggtatcaa catccaaatg
     7861 atttccacct ccgaaatcaa agtttccgta ttgattgatg aaaaatacat ggaactggca
     7921 accagggtat tgcataaagc ctttaatttg ggctgatgtt tcacggtttg aaacggacaa
     7981 cggcactgcc tttatcgggc agtgccgttt tgtctgccgc ccgaatgttc ggacggcata
     8041 ttttgacttt ggaacaaaaa atatagtgga ttaaatttat agtggattaa
//
LOCUS       DAWFLT010000088         8019 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWFLT010000088 DAWFLT010000000
VERSION     DAWFLT010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8019
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.088"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            290..1417
                     /locus_tag="WGR74_001824"
     CDS             290..1417
                     /locus_tag="WGR74_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0906190.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIVIGRDKLFIHPRLMELLRGEGNSFTSFQSLVKA"
     gene            1447..2324
                     /locus_tag="WGR74_001825"
                     /pseudo
     CDS             1447..2324
                     /locus_tag="WGR74_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
     gene            2328..2516
                     /locus_tag="WGR74_001826"
     CDS             2328..2516
                     /locus_tag="WGR74_001826"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ0906191.1"
                     /translation="MDGWYDFRFQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAK
                     SEQRLTALSEWVGQLESE"
     gene            complement(2593..4104)
                     /gene="lysS"
                     /locus_tag="WGR74_001827"
     CDS             complement(2593..4104)
                     /gene="lysS"
                     /locus_tag="WGR74_001827"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ0906192.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNQGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRAVNGTANITYNGKEVDLESPFERLTILDAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIIDY
                     PVEVSPLARASDSKPGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(4101..4253)
                     /locus_tag="WGR74_001828"
     CDS             complement(4101..4253)
                     /locus_tag="WGR74_001828"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906193.1"
                     /translation="MPSETANRFQTALPRLWKKMPTSRKIRANFVIRSGRLKQIAPDL
                     FFRKTL"
     gene            complement(4259..5512)
                     /locus_tag="WGR74_001829"
     CDS             complement(4259..5512)
                     /locus_tag="WGR74_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0906194.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQSDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            5970..7766
                     /locus_tag="WGR74_001830"
     CDS             5970..7766
                     /locus_tag="WGR74_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ0906195.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPDDLLDQVWTSRPAIPAETVFVH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSYLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
BASE COUNT         2000 a         2245 c         2031 g         1743 t
ORIGIN      
        1 tccactaaat agtgtatatt aagtacgtct gatatacgcg ataccctccg agggtgtaaa
       61 ctttagttca catttaaaat gacctcttta aacctgcctt tcggcaggtt tctttttagg
      121 ttgtttggaa atcgtgtgca gacaaggtgt aaaataggta acaacataaa ataatgcggt
      181 tttaccgccc atatatttac aaaagccaaa tttttaaaca tatatccttg atatatacac
      241 ggcgtaaata tatactggaa acatctttaa attttccgaa attttaaata tgagcaactg
      301 gaaacccgat attccctata acgatttacc acccctgccg ccaaaacagg atattgaaag
      361 caaaaccatc ctgaaacgtt gtatagccgc ccgtgcatcc cttgcccgtt taaagcaggc
      421 ggcagaattg ataccgaatc aagccatgct gattaacacc cttcctgtta tggaagcccg
      481 tgcaagttcg gaaattgaaa acatcgtaac cacaacggac aagctgtttc aatccctgca
      541 aatggatacg gaacggcaag accctgccac gaaagaagcc ctgcaatacc gcaccgccct
      601 gtttgcaggc tatgaatcac tgacgagccg ccctttatgc acacaaaccg ccatcatggt
      661 ctgcaacgcc atcaagcacc cctacgaaat ggccatccgc aaaacaggcg gcacagccct
      721 aaaaggaggc aacagcggaa atgttgtcta taccccgccc gaaggagaag aaaccatacg
      781 cggcaagctg gcaaattggg agcggtttat tcacgaaagc ggcgatttag acccgcttat
      841 catcatggcg gcggcacatt accaatttga agccatccat ccgtttacgg acggcaacgg
      901 gcggacgggg cgcatattga acagcctgct attgattgaa aaagggcttt tggatttgcc
      961 tattttgtat ttgagccgct acatcatcga aaacagggcg gactattacc gcctgctttt
     1021 aggcgtaacc gaacggcagg attgggaaag ctggataatc tacatcttag acggcgtagc
     1081 cgataccgcc gattggacgg tatcgaaaat agatgcgata cgccgcctgt tcgagcagac
     1141 acggcaacac atacggacac acgcacaagg aatctacacg cacgaactgg taaatcttct
     1201 gtttgagcag ccatatacac gcattgccaa cctagaagcg gcagggatag ccaaacggca
     1261 gacggcctct aagtacctga aagagctttc agacataggt gtgctgcaag aaatcgtcat
     1321 cggcagggac aaactattca ttcatccgcg cctaatggaa ctattgcggg gagagggcaa
     1381 cagctttacc tcattccaat ccctcgttaa agcatagcca aaataatcaa taatccggag
     1441 gtcaatatgg caagaaggtc aaaaacattt gaagaagctg ctgctgaggt tgaggaacgt
     1501 ttcggtcatc gtggcattaa gttggtcgag tttgagggta cagccaagcc gtgtgtaatc
     1561 aactgcccta aacatggaaa ccaaacctgt tcgaggtact ccaatatgtt cataggaagt
     1621 agctggggtt gcccctcttg tggtaatgag caagctgcaa aagccggtat agcgaccctt
     1681 aggaagaatc acatagcgtt agaaatgctg aaacaggctg taacaggtat gaccaagcaa
     1741 gagcgcatca cgacgcaagc ctacaatgag atgaccaaat ccgtggcagg ttcaaacagc
     1801 atagtcctta acgatgtcca aggcgatacg accatcaaca accatcatac gcatacgcac
     1861 aaccacagcg atgccgacgg caaagcactg tcgatgaggc tcacaccacg ccccctgttg
     1921 tcagaccgtc aggcggcggc tttcgcccgt acaggcaaac tcacaggcag tttcgacttg
     1981 tttgcttcgg tggtcgcccc ctcgcagtac acgtttgccg ttgccatgcc cgacacgtcc
     2041 atgtcgccgg ttatcgaaaa aggggatttg gtggtggtcg agccgcgtat gcgccctgcg
     2101 gacgaggaca tcgtgctgat tgaactgtcc gacaagcggc tggtcgtcgc gcaccttgtt
     2161 atcgatattg cgggcaggat gctgatttat cagacgggca ggccgtctga agcctttgac
     2221 ctgcccgaag gcagcacgat tttaggtgtg gtgctggagt caaaaaacgg tttatgtccg
     2281 ccgcacaggc aagaaggcgt gttgattcgg attaccaccc ctgatgtgtg gacggttggt
     2341 acgatttccg cttccaaaac gtcgtgtacg cgcccgaccg cagcccggaa atcagccgta
     2401 tgctttcttc gattttggca ggctacgcgt gggataccga aaacccgttc gtggcgaaat
     2461 ccgaacaacg cctgactgcc ttgtccgaat gggtcggtca gttagaaagc gaataaatcc
     2521 gtaccgccat acaaaatgcc gtctgaatcc aatcgggttc agacggcatt gccatttcaa
     2581 ctgtttttat gactactcgg ggcgcatctg cggaaacaga atcacgtcgc ggatggtttg
     2641 cgaatcggtc agcaacatga ccaagcggtc gatgccgatg ccgcagccgc cggtcggcgg
     2701 caggccgaat tccatcgcgc ggatgtagtc ggcatcgtag tgcatcgctt cgtcgtcgcc
     2761 cgcgtctttt tgcgccactt gtgccttgaa gcgttcggct tggtcttccg gatcgttcaa
     2821 ctcggagtag ccgtttgcca gttcgcggcc gacaacgaaa agctcgaagc gttcggtcaa
     2881 gcccggtttg ctgtcggatg cgcgtgccaa cggggacact tcgacagggt agtcgataat
     2941 gaaagtcggg ttccacaatt tgccctcggc gcaaccttca aacagggcaa gttgcaggct
     3001 gccgatgccc ggagacggag gcaggctttc gccgtgtttg acgatttctt ttttcagcca
     3061 ctccgcatcg ttcaactgct cgtcggtgta gtgcggattg tattttttga tggcatcgag
     3121 aatggtcaga cgctcgaacg ggctttccaa atcgacttct ttgccgttgt aagtgatgtt
     3181 tgccgtgccg tttaccgcgc gggaagcgtt gcggatgatg tcttccgcca tttgcatcat
     3241 gcgttcgtag tcggagaagg cttcgtagaa ttcgatcatg gtgaattcag ggttatggcg
     3301 cacagacata ccttcgttgc ggaagctgcg gttgatttca aacacgcgtt ccaaaccgcc
     3361 gacaaccaag cgtttcagat acagctcggg ggcgatgcgc aggtagagcg gaatgtccaa
     3421 ggcattgtgg tgggtaacga agggttttgc cgtcgcgccg ccgggaatcg ggtgcatcat
     3481 cggggtttcg acttcgagat aatgctcgcc caccataaaa ttacgcacgg attggatgat
     3541 ttggctgcgt ttgataaagg tattgcgcga ttcttcgttg gcaatcaaat cgacatagcg
     3601 ttggcggtat ttggtttcct gatcgctcaa gcctttatgt ttgtccggca gagggcgcag
     3661 ggatttggac agcaggcgga tgccggacac gcgtacggtc agttcgccct ggttggtttt
     3721 gaacaaagtg ccttccgcgc cgacgatgtc gcccaaatcc caatggttga agtcgtccaa
     3781 aacttcttgg ctcacgcctt tgttgttcag ataaagctgg atttgcccgg acacgtcttg
     3841 aatagtggca aagctcgcct tgcccatttg acgcttcagc atcatgcggc cggccacttt
     3901 gacgggaatg ccttgcggat cgagttcttc tttgccgatt tcgccgtatt gggcgtgcaa
     3961 atcggcggcg aagctgtcgc gtttgaagtc gttgggatag gcgttgcgct gttggcggat
     4021 gttgtgcagt ttttcgcggc gcagggcgat gatttggttt tcgtccaact gcggctcggt
     4081 ttgcggatgg ttttgttcgc tcatagggtt ttccgaaaaa ataaatcagg cgcaatctgt
     4141 ttcagacgac ctgaccgaat cacaaaattt gcgcgtattt tacgcgatgt cggcattttt
     4201 ttccataaac gcggcaatgc cgtctgaaag cggtttgcgg tttcagacgg catcgttatc
     4261 atttgaacat tcccgccaaa ttcaataaga acaaaacggt aaaaccggtc agataaatca
     4321 agcctgccaa tgcaagggca ttcatacctg atgtgagttt gtgtttttca tcacctttga
     4381 ccaaacggta attcagccag gcaaacacag gagcggacac aaaagcggca atcatcgcaa
     4441 atttgagcag attcgccatt acgccgtcaa accaaaaaat caccgccaaa ccgctgcccg
     4501 ccacccaaat attccaggca aagaattcgg cgttgcccgt tttgtctttt ccgcgcagca
     4561 ggcgcacggg ttcggcaatg gcacgggcat agccgtccac gacggtaatc gtcgtgccgt
     4621 acatacaggc aaacgcgata aacgccacca gcgggcgcga ccagccgccg atggtaacgg
     4681 cgtacatatt gatcaattgc ccgatatatt tgccgcccgc catctgtact gcttcgccgt
     4741 tgccgtattg cacaaacgca cccagtgcaa ggaaaaccaa agccaaaacc gcactggcga
     4801 tataaccgac gttgaaatca aaaatcccgt cgcggtattc ggaaggattg atgcgttgtt
     4861 tttcggttac ccacaaagaa ttgatggcgg aaatttcaat cggcgcgggc atccagccca
     4921 tcagcgcgat caggaagccc aaaccggcaa gcgtccacgg tgtcggctcg ataaaatcgg
     4981 actgcatctg cataccgcgc gacatagcga tgccggcggc ggcaagcgtg gcgatactca
     5041 aagtaacgat gatgattttg gaaacgcgat ccaaagcgcg gtaacgtccg ctcaccaaaa
     5101 taatcaggca ggatgccata atcaaggcgg caaccgtgcc ggcatcaaac atcagcgagg
     5161 gaatcgccat tttgacgatg gcggcggtta caatggcgac cgcgcccgcg ttaatcgtgg
     5221 cggagaggat gcacaaaatc aggaataccc acaaataaac gcggcttttc tcggcataac
     5281 cttcaatcag gctcttgccc gtgtccagcg tgtaatgcgc gctgaagcgg aaaaacgggt
     5341 atttgaagag gttggtcagg atgatgatga gcgcgatctg ccagccgtaa agcgcgcccg
     5401 cctgcgtcga ggcaatcagg tgcgaaccgc cgaccgccgc cgaagccatc ataatccccg
     5461 gtcccaatgc gttgatttta cttttccaag tcgaaatatg ttgttcggac ataaagtctt
     5521 ccgtattttt aaccgtgttt caacacacag agccgcatat tcggacacag ccctatctat
     5581 tgctccaatt tgggcgggat tgcccccaaa caaacccaaa tcctaccgtc ttcaaaaaca
     5641 ggataccgcc cggtagggaa attttgatga aagcgcgtat tgtaacgtaa tccaaatacc
     5701 tgccaacaca cactattaga acttcatgct caaacttgac tatattttcc gtattacttc
     5761 caaaaaaatg cataaaacga tatcttatgc ctaaaatttt acaacaaaca accttacatc
     5821 gcttttttcg cgcaaacacg caccatccga tcagcccgtc cgttttgcag caggctggcg
     5881 atttgataag atggttatgt ttttcagacg gcatttcaga tttccgtcca tgccgtctga
     5941 agccgcaaaa cccgattgga ggaactgtta tgaataccgt atcgaattac ctgtccgcat
     6001 tgcgcgaagc catgaaggcg caaggcttgg atgcactcgt catcccttcc gccgaccccc
     6061 acctgtccga atacctgccc gagcattggc aggcgcgccg cgaattatcg ggctttaccg
     6121 gctcggtcgg cacgtttgtc ctgaccaccg atgaagcggg cgtatgggtg gacagccgct
     6181 attgggaaca agccgccaag cagcttgcgg gcagcggcat cgaactgcaa aaaagcgggc
     6241 aagtgccgcc gtataacgaa tggctcgcgg caaacctgcc cgaaaacgcc gccgtcggca
     6301 tcccttccga tatggtctcg ctcaccggca aacgcacttt ggcgcaatcc ctgaccgcca
     6361 aaaacatccg catcgaacac ccggacgatc tgcttgacca agtgtggaca agccgtcccg
     6421 ctatccccgc cgaaaccgtg ttcgtccacg accccgccta cgtctccgaa accgccgccg
     6481 aaaaactcgc ccgcgtgcgc gccgtgatgg cggaaaaagg cgcggattac cacttggttt
     6541 cctcgctcga cgacatcgcc tggctgacca acctgcgcgg cagcgacgtg cctttcaatc
     6601 ccgttttcgt gtcctatcta ttgattggca aagacaacgc cgtcctgttt accgaccgat
     6661 gccgtctgaa tgccgaagcc gccgccgcgc tgcaaaccgc aggcatcacg gtcgagcctt
     6721 acgcccaagt tgccgacaaa ctcgcgcaaa tcggcggcgc gttgcttatc gaaccgaaca
     6781 aaaccgccgt cagcacgctt gtgcgcctgc ccgaaagcgt gcgccttatc gagggaatca
     6841 acccgtccac gctgttcaaa tcctgcaaat ccgaagccga catcgcccgc atccgcgaag
     6901 cgatggaaca cgacggcgcg gcgttgtgcg gtttcttcgc cgagtttgaa gacatcatcg
     6961 gcaacggcgg cagcctgacc gaaatcgacg tggacaccat gctttatcgc caccgcagcg
     7021 tgcgcccagg cttcatttca ttgagtttcg acaccatcgc aggcttcaac gccaacggcg
     7081 cactgccgca ttacagcgca acgcccgaaa gccacagcac catcagcggc aacgggcttt
     7141 tgctcatcga ctccggcgcg caatacaaag gcggcacgac cgacatcacc cgcgtcgtcc
     7201 ctgtcggcac acccaccgcc gaacaaaaac gcgacaacac cctcgttctc aaagcccata
     7261 tcgcgcttgc cgaagccgtg ttccccgaaa acatcccctc gccgctgatt gatgcgattt
     7321 gccgcaaacc cctgtggcag gcgcaatgcg actacggcca cggcaccgga cacggcgtag
     7381 gctatttcct caacgtccac gaaggcccgc agcgcatcgc cttcgccgcc cccgccacgc
     7441 ccgaaaccgc catgaaaaaa ggcatggtta cctccatcga acccggactc taccgcccgg
     7501 gaaaatgggg catccgcatt gaaaaccttg ccgccaacca agccgtcgcc gcccctcaag
     7561 aaaccgaatt cggcagcttc ctctgttttg aaaccctgac cctctgcccc atcgacaccc
     7621 gcctgatgga caccgccctc atgaccgacg gcgaaatcga ctgggtcaac cgctaccacg
     7681 ccgaagtccg ccgccgcctc gagccgctga ccgaaggcgc ggcaaaagcg tggctgatca
     7741 aacgcaccga accgctggcg cgttaaacag cacggcgcaa aaaatgccgt ctgaaagccc
     7801 ttcagacggc attggtttcc caaaacatcc cgcaccgttt tcatcttgcc gcaagcaaat
     7861 atagtggatt aacaaaaatc aggacaaggc gacgaagccg cagacagtac gaatagtacg
     7921 gaaccgattc actcggtgct tcagcacctg agagaatcgt tctctttgag ctaaggcgcg
     7981 acaacgccgt actggttttt gttaatccgc tatattccg
//
LOCUS       DAWFLT010000089         7928 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWFLT010000089 DAWFLT010000000
VERSION     DAWFLT010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7928
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.089"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(27..443)
                     /gene="dksA"
                     /locus_tag="WGR74_001831"
     CDS             complement(27..443)
                     /gene="dksA"
                     /locus_tag="WGR74_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ0906196.1"
                     /translation="MAKLTEQDILNWNGPEDDYMNDEHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(590..1381)
                     /locus_tag="WGR74_001832"
     CDS             complement(590..1381)
                     /locus_tag="WGR74_001832"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ0906197.1"
                     /translation="MNVYFLGGGNMAAAIAGGLVKQGGYRIYIANRGAEKRERLEKEL
                     GVETSATLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVGTLSRYLGG
                     TRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLDDEEKMHGI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1430..1838)
                     /locus_tag="WGR74_001833"
                     /pseudo
     CDS             complement(1430..1838)
                     /locus_tag="WGR74_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            complement(1838..2524)
                     /locus_tag="WGR74_001834"
     CDS             complement(1838..2524)
                     /locus_tag="WGR74_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ0906198.1"
                     /translation="MTVLQERYCEVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSEQRPSSMPPLQVCIEVNIAGEAVKHGVAPEEAVALAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNAAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2644..3687
                     /locus_tag="WGR74_001835"
     CDS             2644..3687
                     /locus_tag="WGR74_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ0906199.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMNA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALSSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3850..5076
                     /locus_tag="WGR74_001836"
     CDS             3850..5076
                     /locus_tag="WGR74_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0906200.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5362..7512
                     /gene="yccS"
                     /locus_tag="WGR74_001837"
     CDS             5362..7512
                     /gene="yccS"
                     /locus_tag="WGR74_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ0906201.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIITTVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAILLFQIVLPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNAAQALRSGKDYTYSKRLGRAIEGCRQSLRLLSDGNDNPDIRHLGRLLDNLGS
                     VDQQFRQLQHNSLQVENDHMGDTRIAAIETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPTKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1951 a         2620 c         1721 g         1636 t
ORIGIN      
        1 ttcggacggc attttgtttg ccgccatcag tcggcaaact gttttttcat cctctctcgg
       61 cgttcttggg actcaacaga taaagtggct gtcgggcgtg ccagcagccg cttcagaccg
      121 ataggctctc ccgtatcggc acagaatcca taatcccctt catcaatatt gcggatggtc
      181 gcctgtattt tactgagaag ttttcgttcc cgatcgcggg tacggagttc caatgcgtac
      241 tcttcttcct gtgtggcacg gtcggcagga tcgggggctg attcgtgttc ttggagatgc
      301 cctgtcgtag cggaagcatt ttcgatgagt tcgtcttgca tttttaccag caattcgcgg
      361 aaaaaagcca aatgctcgtc attcatataa tcgtcttccg gcccgttcca attcaaaata
      421 tcttgttctg tcagctttgc cataatgtat atcctctcgg ttcaccgaaa aaaacaaagc
      481 accacaaagt ggctttattc ctttgatttt tataaggctt attgcctagg gcgtttttga
      541 aaacgcgcat cataacacgt tttggattgg tttttttatt ttctttacat tattgatatt
      601 gccgttccat ttcctgcgaa cggcgcacac aggcacaaac gccctcactt atggcttcgg
      661 cgacacgatg ccgcctgaag gcttccacgg cttcgtgggt tgtcccgcct tttgacgtta
      721 catttttttg aagcttctcg aaatcttcac ccgtctgctc ggcaagggca accgctcctt
      781 taaacgttgc cagactgagc gcgcgtgctt ctgccatatc aaacccttgt cggatggcgg
      841 cattttgcaa tgcgtccagc agataaaaca cataagccgg tccgctgccg ctgatgccgg
      901 taatgccgtg cattttttcc tcatcatcca accaaacagt caaaccgact gatttcatga
      961 ttcgatcggc aatcctgcgg tctgtttccg atacttccgc ttcggcatac ataccagata
     1021 cgcccagccc gatttttccg ggtgtattcg gcataacccg gacaatgcgg cgtgttcccc
     1081 cgaggtaacg gctgagcgta ccgaccgaca atccggctgc gacagaaagc accaatgcgc
     1141 cgttggtgcg gatatttttg cacgcggctt ccatatcctg cggtttgacg gcaaggatta
     1201 aaacatcgtc ggaatgaagc tccggcaggg ttgccgaagt ttcgaccccc aactcttttt
     1261 ccaaacgttc gcgtttttcc gcaccccgat tggctatata gatgcggtaa ccgccttgtt
     1321 tgaccaatcc gcctgcgatg gcagccgcca tattgccgcc gccgagaaaa taaacattca
     1381 ttatttctac cctattcaaa attaccttac taaattcatt cccaattttt taccatcgga
     1441 cacgctcgga cagaccgttg cgccgcaacc cctgcttttc caagcagtca aatcctccct
     1501 gtttgaacgt ctcaatcaaa ggtttgccgt cacgcgtacg ccaatccagc ccgcaggctt
     1561 caaacttccc tattttcctg taaaagtaag attgttgtgc ggattcatat tcgtttgccc
     1621 tgacggaacg attgccgtct gaaagcggta tctcgtagtc ctctaaagac ggcggactct
     1681 cgccgccaat atcccaaaaa tcgatggcag ccggttttct cggcttacac cagccggaca
     1741 gggacgaaca ctcaatacca acccgccaac cggcagcctg caaccgtaaa tgcaagcaac
     1801 gcagccattg ccgtatattt tattgcccga atttccatca gccccttttc ccgaaaatcg
     1861 cgctgccgat acggacgtgt gtcgcaccgc actcaatggc ggcaggcata tcgtccgaca
     1921 tccccataga cagcacgtct gccttaacgc cagccgcatt gaggtcggca agcagtttcc
     1981 gcatcgtttg aaattgcacc ttcaactccg tttcactgct gttggctttg gcaacacaca
     2041 tcagtccgcg tacgacgata ttcggcagct tcgccacttc cacagcaagc gcgactgctt
     2101 cttcgggcgc gacaccgtgc ttcaccgcct cgcccgcaat gttcacctca atacacacct
     2161 gcaaaggcgg cattgaggaa ggacgctgtt cgctcaaccg gacagcggtt ttcagacggc
     2221 atacggtatg cacccaatgc gcgcgttcgg cgacaaactt ggttttgttg gactgcacat
     2281 cgccgatgac gtgccacacg atgtcggtca aatccgccaa ctcttccgtt ttgccgtacc
     2341 actcctgaat atagttctcg ccgaaatcac gctgtccggc ggcgtaaact tcgcggatgc
     2401 cgtctgaagg gaaagtctta ccgacggcaa tcaggctgac ggaatgcggc ttccttcccg
     2461 cctgcagaac caattttccg atacggtcgg acacctcaca ataacgttct tgcaacaccg
     2521 tcatagatta tcccctaatt aaaaatgatt aaacagttga aacctctcca gtcagggcgg
     2581 tacaatcaag gttgttagaa ccattccaac caatcgaaac attatactaa acagagccgc
     2641 attatgcaga ttaccgactt actcgccttc ggcgctaaaa acaaagcatc cgaccttcac
     2701 ctgagttcgg gcatatcccc tatgattcgg gttcacggcg atatgcggcg catcaacctt
     2761 cccgaaatga acgcggaaga agtcggtaat atggtaactt cggtgatgaa cgaccaccag
     2821 cggaaaatct accagcaaaa cttggaagtc gacttctcgt tcgaactgcc caacgtcgcc
     2881 cgattccgcg tcaacgcctt caacaccggc cgcggtcccg ccgccgtatt ccgtaccatt
     2941 cccagcaccg tcttatcgct ggaagaattg aaagccccga gcattttcca aaaaatcgca
     3001 gaatcgccgc gcggcatggt attggttacc ggccctaccg gttcgggcaa atcgaccacg
     3061 cttgccgcga tgatcaacta catcaacgaa acccagccgg cacacatcct gaccatcgaa
     3121 gacccgatcg aattcgtcca ccaaagcaaa aaatccctga ttaaccagcg cgagctgcac
     3181 cagcacaccc tcagcttcgc caacgcgctg agttccgcat tgcgcgaaga ccccgacgtt
     3241 atccttgtcg gcgagatgcg cgacccagaa accatcggct tggcactgac cgccgccgaa
     3301 accggacact tggttttcgg cacgctgcac acgaccggcg cagcaaaaac cgtcgaccgt
     3361 atcgtggacg tattcccggc gggagaaaaa gaaatggtgc gctctatgct gtccgaatcg
     3421 ctgaccgccg tcatctccca aaacctgctg aaaacgcacg acggcaacgg ccgtgtcgcc
     3481 tcgcacgaaa tcctgattgc caaccccgcc gtccgcaacc tcatccgcga aaacaaaatc
     3541 acgcagatta actccgtcct gcaaaccggg caggcgagcg gtatgcagac aatggaccaa
     3601 tcgctgcaat cgctggtgcg ccaagggctg atcgcaccgg aagtcgcacg cagacgcgcg
     3661 caaaacagcg aaagtatgag tttctgacac acaaccgcct tccggccata ccggcgggaa
     3721 aacaaggcgc aaacacgcga ggcgggacgc agcatcccgc ccggctacct ttccgaacaa
     3781 gaagcgtccg ccttcctgtt gaaacctgcc gccgcaaact gcaaggctta aaccgaaaag
     3841 aagctagcga tgaataccga taacctgcac gacatcttgg acgaaatggt tcaagtgtat
     3901 tctcaaaaaa aacaaagccg atccgaaacc ccggccgaaa tcggcgcaca cttccacccg
     3961 ctgctcgacc gcctgtgcga aaccgcagaa gcacaaaacg cgtccgacat ccttatcagc
     4021 aaaggattcc cgccctcgtt gaaaatcaac agcgcattaa ccccgcagcc gcaaaaggcg
     4081 ctgacgggcg aggaaaccgc cgccatcgcc gcatcgacga tgaacgccga acaatcggaa
     4141 atattccggc gcgacggcga aatcaactac tccgtccagt cgcgcagcgg cacgcgctac
     4201 cgcgccaacg cctaccacag ccaaggcagc gcaggtttgg ttttgcggcg catcaaccac
     4261 gtcatcccgc aaatgcagga attgggcctg cccgaaaaac tcaaagacct cgccgtcgca
     4321 ccgcgcgggc tgctgattat cgtcgggcct accggttcgg gcaaatccac cacgatggcg
     4381 actatgctcg aacaccgcaa caaaaccctg cccagccata tcgttaccat cgaagacccg
     4441 attgagttta tctacaaacc gcgccgctgc atctttaccc agcgcgaaat cggcgtcgac
     4501 accataaact ggcagacggc ggtacaaaac gctatgcgcc agtcccccga cgtggtctgc
     4561 atcggcgaag tccgcagcag ggaaagtatg gaatacgcga tgcagctcgc ccaaaccggc
     4621 cacctgtgca tttttacgct ccacgccaac accgcgccgc agtcgctcga acgcatactc
     4681 aacttctacc ccaaagaaca gcacaaccaa atactgatcg acatcgccct caacctgacc
     4741 ggcatcatct gccaacgcct cgccctcaaa caagacaaaa cgggcaggac ggcggttgtc
     4801 gacttgctca tcaacacgcc cgccatccaa gacttcatcc tgaagggcga cctgatgaac
     4861 atcagtaaaa tcatggaaac cgccaaaacc gacggaatgc agacgatgga tcaaaacctt
     4921 ttcgaactgt accgtcacgg catcatcagt tacgaagaag ccctgcgcca gtccgtttcc
     4981 gccaacaacc tgcgattgca catccaactg cacaaagaag gcaaaacgcc cgaactcctt
     5041 tacgacaggg tcaacggtct caacctcatt tcctgatccg caaaacccaa tgccgtctga
     5101 aaaccgcatc cccgttttca gacggcatga ttttatccgc cccattcatg tgctacactt
     5161 tatagtggat taaatttata gtggattaac aaaaatcagg acaaggcgac gaagccgcag
     5221 acagtacaaa tagtacggaa ccgattcact tggtgcttca gcaccttaga gaatcgttct
     5281 ctttcagcta aggcgaggca acgccgtact ggtttttgtt aatccactat attcacttca
     5341 taacaataaa ccggtaaaac catgaaaacc ccactcctca agcctctgct cattacctcg
     5401 cttcccgttt tcgccagtgt ttttaccgcc gcctccatcg tctggcagct aggcgaaccc
     5461 aagctcgcca tgcccttcgt actcggcatc atcgccggcg gccttgtcga tttggacaac
     5521 cgcctgaccg gacggctgaa aaacatcatc accaccgtcg ccctgttcac cctctcctcg
     5581 ctcacggcac aaagcaccct cggcacaggg ctgcccttca tcctcgccat gaccctgatg
     5641 accttcggct tcaccatttt aggcgcggtc gggctcaaat accgcacctt cgccttcggt
     5701 gcactcgccg tcgccaccta caccacgctt acctacaccc ccgaaaccta ctggctgacc
     5761 aaccccttca tgattttatg cggcaccgta ctgtacagca ccgccatcct cctgttccaa
     5821 atcgtcctgc cccaccgccc cgtccaagaa agcgtcgcca acgcctacga cgcactcggc
     5881 ggctacctcg aagccaaagc cgacttcttc gaccccgatg aggcagcctg gataggcaac
     5941 cgccacatcg acctcgccat gagcaacacc ggcgtcatca ccgccttcaa ccaatgccgt
     6001 tccgccctgt tttaccgcct tcgcggcaaa caccgtcacc cgcgcaccgc caaaatgctg
     6061 cgctactact ttgccgccca agacatacac gaacgcatca gctccgccca cgtcgattat
     6121 caggaaatgt ccgaaaaatt caaaaacacc gacatcatct tccgcatcca ccgcctgctc
     6181 gaaatgcagg ggcaggcatg ccgcaatgcc gcccaagcac tcaggtcggg caaagactac
     6241 acatacagca aacgcctcgg ccgcgccatc gaaggctgcc gccagtcgct gcgcctcctt
     6301 tcagacggca acgacaatcc cgacatccgc cacctcggcc gcctgctcga caacctcggc
     6361 agcgtcgacc aacaattccg ccaactccag cacaacagcc ttcaagtgga aaacgaccac
     6421 atgggcgaca cccgcatcgc cgccatagag accggcagcc tcaaaaacac ctggcaggca
     6481 atccgtccgc agctaaacct cgaatcaggc gtattccgcc atgccgtccg cctgtccctt
     6541 gtcgttgccg ccgcctgcac catcgtcgaa gccctcaacc tcaacctcgg ctactggata
     6601 ctactgaccg cccttttcgt ctgccaaccc aactacaccg ccaccaaaag ccgcgtccgc
     6661 cagcgcatcg ccggcaccgt actcggcgta atcgtcggct cgctcgtccc ctactttacc
     6721 ccctccgtcg aaaccaaact ctggattgtc atcgccagta ccaccctctt tttcatgacc
     6781 cgcacctaca aatacagttt ctccaccttc ttcattacca ttcaagccct gaccagcctc
     6841 tccctcgcag gtttggacgt atacgccgcc atgcccgtgc gcatcatcga caccattatc
     6901 ggcgcatccc ttgcctgggc ggcggtcagc tacctatggc ccgactggaa atacctcacg
     6961 ctcgaacgca ccgccgccct tgccgtatgc agcaacggcg cctatctcga aaaaatcacc
     7021 gaacgcctca aaagcggcga aaccggcgac gacgtcgaat accgcgccac ccgccgccgc
     7081 gcccacgaac acaccgccgc cctcagcagc accctctccg acatgagcag cgaacccaca
     7141 aaattcgccg acagcctgca acccggcttt accctgctca aaaccggcta cgccctgacc
     7201 ggctacatct ccgccctcgg cgcataccgc agcgaaatgc acgaagaatg cagccccgac
     7261 tttaccgcac agttccacct cgccgccgaa cacaccgccc acatcttcca acacctgccc
     7321 gaaaccgaac ccgacgactt tcagacagca ctggatacac tgcgcggcga actcgacacc
     7381 ctccgcaccc acagcagcgg aacacaaagc cacatcctcc tccaacagct ccaactcatc
     7441 gcccggcagc tcgaacccta ctaccgcgcc taccgacaaa ttccgcacag gcagccccaa
     7501 aacgcagcct gaaaaagttt cggcattttg taagagaggg cagattgtca gacaggttgc
     7561 aagatagtgg ataaagcttt gcgcagggta aatgcgtagc aactgaaccg tcattcccac
     7621 gaacctacat cccgtcattc ccacgaaaac agaaaaccaa aaacagaaac ctaaaatccc
     7681 gtcattccca cgaaagtggg aatccagttc gttcggtttc gcttgtttta agtttcgggt
     7741 aacttccact tcgtcattcc cgcgcaggcg ggaatccagt gcgttgagct tcagctattt
     7801 agaataaatt ttgaaactct aatcccgtca ttcccacgaa cctgcatccc gtcattccca
     7861 cgaaagtggg aatccagaat ctcggacttt cagataacct ttgaatatta ctgttgttct
     7921 aaggtcta
//
LOCUS       DAWFLT010000090         7890 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWFLT010000090 DAWFLT010000000
VERSION     DAWFLT010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.090"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            296..1609
                     /locus_tag="WGR74_001838"
     CDS             296..1609
                     /locus_tag="WGR74_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ0906202.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
     gene            1828..2003
                     /locus_tag="WGR74_001839"
                     /pseudo
     CDS             1828..2003
                     /locus_tag="WGR74_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            1999..3249
                     /locus_tag="WGR74_001840"
     CDS             1999..3249
                     /locus_tag="WGR74_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ0906203.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRIFVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTEQGRIAV
                     MQENEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQSAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEVSADETEIYTLIKTFADNAVRYTPPEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERARVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGHL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(3284..4777)
                     /gene="rng"
                     /locus_tag="WGR74_001841"
     CDS             complement(3284..4777)
                     /gene="rng"
                     /locus_tag="WGR74_001841"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ0906204.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            4991..5248
                     /gene="grxC"
                     /locus_tag="WGR74_001842"
     CDS             4991..5248
                     /gene="grxC"
                     /locus_tag="WGR74_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ0906205.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            5269..5712
                     /gene="secB"
                     /locus_tag="WGR74_001843"
     CDS             5269..5712
                     /gene="secB"
                     /locus_tag="WGR74_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ0906206.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            5798..7840
                     /gene="recG"
                     /locus_tag="WGR74_001844"
     CDS             5798..7840
                     /gene="recG"
                     /locus_tag="WGR74_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ0906207.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGTQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAIGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMAEFAAGRLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
BASE COUNT         1649 a         2064 c         2337 g         1840 t
ORIGIN      
        1 tcccgcgtgt acggcacgcc ccgcgctccg caatgatagg ggcggtttcg tttgaatcgg
       61 cgcaccggga tgaatgcccc cgcgccgtcc cgataccgat ttgccggttg ttccgtatcg
      121 gttgccgccg tgaaatgacg gacggatgca ggaatggcgg caggcgtgaa tgggaggaaa
      181 tgaccgattg acttgtttca aaataaaaaa gtagaatgca gggcttatcc cgcgtctgtg
      241 ggcggttttg cctgcacggt cggttttctg tttaaaataa aataaaggag aaatgatgct
      301 gacgtttatc ggactgctga ttatcggggt catcgtatgg ttgctgctga cggaaaaagt
      361 gtcgcccatc atcgcattaa tcttggtgcc gctgtttggg gcgttgctgg cggggtttga
      421 tgtatcccaa ttaaaagaat tttattcggg cggcaccaaa tcggtgatgc agattgtgat
      481 tatgtttatg ttttccattt tgttttttgg aatcatgaac gatgtggggc tgtttcgtcc
      541 gatgataggc ggtttgatta agctgactcg gggtaatatc gtggcagtga gtgtggggac
      601 ggtcttggtg tcggtggtgg cgcagttgga cggggcgggt gcgacgacgt ttttattggt
      661 cgtccccgcc cttttgccgc tttacaagcg tctgcatatg aatccttacc tgctgttttt
      721 gctgctaact tccagtgcgg gattgattaa ccttctgccg tggggcgggc cgactgggcg
      781 ggttgcaagc gtgttgggcg cagatgtggg cgaattgtat aaacctttgt tgacggtgca
      841 aattatcggt gtagtgttta tccttgcgct gtccctgctt ttgggtgtgc gtgaaaaaag
      901 gcggattgtc cgggagttgg gcgcgttgcc cgccgtggcg gatttgataa agccggtgcc
      961 tttgtcggaa gaagaacaaa aattggcgcg tccgaaactg ttttggtgga atgtcctgct
     1021 gtttttggcg gcgatgagcc tgcttttttc gggcatcttc ccgccgggtt atgtatttat
     1081 gctggctgca acggcggcgt tgcttttgaa ttaccgcagc ccgcaggaac agatggagcg
     1141 gatttatgcc cacgccggcg gcgcggtgat gatggcgtcc attattttgg cggcaggtac
     1201 gtttttgggg attttgaagg gtgcggggat gttggacgcg atttccaaag acattgtgca
     1261 tatcctgccg gacgcgctgc tgccttatct gcatattgcc atcggtgtgt tgggcattcc
     1321 gcttgagttg gttttgagta cggacgctta ttatttcgga ctgtttccga ttgtggagca
     1381 gattacctcg caggcgggcg tggcgcccga agcagcaggt tatgcgatgt tgatcggcag
     1441 tatcgtcggc acttttgtta cgccgctttc gccggccttg tggatgggct tgggtttggc
     1501 gaaattgtcg atgggcaaac acatccgtta ttcgtttttc tgggcgtggg gtttgtcgct
     1561 ggcgatattg gccagttcga tagcggcagg aatcgtgcct ctgccgtaaa cggcgaagcc
     1621 gtctgaagcc tgaatcgttc ctgtggtctg tcgcaggctc tgcctgcccg tgtgttgcaa
     1681 tcgcgcccca atgccgtctg aaggcagttg ccggcgcggt ttttttgtgc cgcagttttg
     1741 aaaaataccg gcaagcgtaa aggccgctaa attgcggcta attttggggt gttagaatag
     1801 gcacggttta ttttgaaggg aaagttgatg cgtgttttgc tggtggaaga cgatgcgatg
     1861 attgcggagg cggtgtcggc aagtttgaaa gacggcggct atgcggtgga ttgggtcaaa
     1921 aacggcgcgc ggctgccgct gcccagcctt atgacttgat gctgctggat ttgggtttgc
     1981 cggggcggga cggtttggat gtttgttccg ctggcaatgc ttgcaggtat gttttcctac
     2041 tacgaaacct tccacgaaac ggaagcgttg caggacgacc tgctccgtca ggcggcattg
     2101 tacgtcgcac ccgattctaa acccgaaact ttgcccgagg gcgacggcga tacgcgcatt
     2161 ttcgtgcaga tgccgcagca ggaagaccct gttgtcagcc tgcctgcgca tctggcggac
     2221 ggtctgcaca cgcttcaggc ggacggggac gacgattatt accgcgtcta tatccgcacg
     2281 accgaacagg gacggattgc cgttatgcag gaaaacgaat accgtgaaga tttggcggcg
     2341 gatgcggcac ggcaaagcgt gttgcccctg ctggcggcac tgccgctgat gatactgctg
     2401 accgtgtgga ttacgcacaa agccatgcgc cccgtccgca aattgtcgca aagtctcgaa
     2461 caacgccgaa tcaatgacct gtctgctttg agtgtggaca atattcccag tgaaatcaga
     2521 gggttcgtaa ccgccatcaa cctgcttttg aaacgtgcgg atgaagacat ccgccaccgt
     2581 cagcggtttg tcgccgacgc ggcacacgaa ttgcgtacgc cgatgacagc cctttccctt
     2641 caggcggaac ggctcaacaa tatgtcgctc ccacccgatg cagcgcggca gtctgccgtt
     2701 ttgcagcaga gcatcaggcg caacaaacac ctgctcgaac agcttttggc actggcgcgt
     2761 tcgcagtcgg atgaaacccc tctgacgaaa acgacattcg gtctgcaaag ccgtttccgc
     2821 caagtgttgc aggaactgat gccgctggct ttggaaaaac gtcaggacat cggcgtggcg
     2881 gtcggaggcg atgtcgaagt gtctgccgac gaaacggaaa tctatacgct gattaaaacc
     2941 tttgccgaca acgcggtacg ttacacgccg cccgaaggca ggatagattt gggtttcacg
     3001 gacgaaggga aatatctcgc cgtgtgggtg gaagataacg ggaacggcat tcccgaatcc
     3061 gaacgcgccc gcgtcctcga tccgttttac cgtattttgg gaacggagca gcaggggacg
     3121 gggctggggc tgtcgattgc cgacacgctt gccaaaaaat acggcggaca tttggaattg
     3181 accgacagcc gacgtttcgg acacggtctg ttgatacggg cgttgttgga caaggaaacc
     3241 ctgaaataga cagatgccgt ctgaaggctt cagacggcat tttttacatc agcacaatat
     3301 cgtattgttc ctgcgtgtaa gcggtttcga ccgccagaga aatcggtttg ccgatgaaat
     3361 ctatcagcat tgccaaggat tgcgattctt cgtccaaaaa caaatcgatg acgttggggg
     3421 cggcgaggat gcggaaactt tcggcatcgt aacggcgcgc ttcgcggacg atttcgcgct
     3481 ggatttcgta gcacacggtt tgcggcgttt tcagacggcc tctgccttgg caggaagggc
     3541 agggttcgca gaggacttgg tttaagtttt cgcgcgagcg tttgcgcgtc agctcgacca
     3601 gccccaggct ggtaaaaccg tgcagggtaa cgcgggtacg gtcgaaggcg agggctttgg
     3661 caagctcctg caacacggct tcgcggtggc tttcctgcgc catatcgatg aagtcgatga
     3721 tgatgatgcc gccgaggttg cgtagcctca attcgcgggc gatggtgtgg caggcttcga
     3781 ggttggtgcg gaagatggtt tcgtcgaagt tgcgtgcgcc gacgaagccg ccggtgttca
     3841 cgtctatcgt ggtcatggct tcggtggatt cgataatcag gtagctgccg aagttgaggt
     3901 tgacgcgcgg ttgcagggcg cggctgattt cctgttcgac gttgtgggtt tcaaacagcg
     3961 ggcgttcgcc tttgaacaat tctatcctgc ccaatgcgcc gtggacgtat tgttcggcaa
     4021 aacgcgtcat gcgcccgtgg tttacggtgg aatcgacgag gattttttgc gtgtcgcagc
     4081 cgaccatatc gcgcaatacg cgcaaactca agggcaaatc ctgataaagc agggtttccg
     4141 gcggccggat tttcgcctgt tcttggatgt gttcccacac tttggtcagg tagtcgatgt
     4201 cggactggag ctgttcgtcg gtggcgtttt cggcgttggt gcggatgatg tagccccggc
     4261 aggcattttc cggcaggagc ttgtcgaggc gttcgcgcag gctgctgcgt tcggcatcgt
     4321 cttcgatgcg ttgggacacg ccgatgtggt cttcttgcgg aagatggacg aggaaacgcc
     4381 ccgccagcga gatttgggtg gaaagccgcg cgcctttggt gttgatcggg tctttgatga
     4441 cctgcaccaa aacagactgc ccttcaaaca gcatatgttc gatgcgctgg gtttcttcgg
     4501 ggttgcggcg ttgttcgagg acatcgacga tgtgtaaaaa cgccgcgcgt tccaagccga
     4561 tgtcgataaa cgcgctctgc atcccgggca gcacgcggcg caccacgccc agatagatat
     4621 tgccgaccag gctgtgttcg ctgttgcgct cgatgtgcag ctcgcagata ttgttttcct
     4681 ccaacaccgc tacgcgcgtt tcctgcggcg tgatgttgac caatatcgtt tcgggcgggc
     4741 gcgcgatgtc tttggggatg gggagtcctg acaacatggt ttttcctgaa aatgtaataa
     4801 aaatattttt gcacccgttc cgccgcgcag cgcgggtgca aaaatatttt tcacaatgcc
     4861 tatcatactt taaaaaagaa actttgacac attccgcaca tgccgtttga acgtgcatct
     4921 gttccgcact tgccaaacag gggaaatgcc cctatattga ttatcattgc aaaactttcg
     4981 gaaacccaat atgcagaccg ttaccatgta cacaggtccg ttttgtccct actgcgcgat
     5041 ggcgaaaagg ctgctgcacg cggcaggtgt cggacatatc gacgaaatcc gtgtcgatgc
     5101 aagccccgaa gcctttgccg aaatgcagca gctttcggga cagcgcagcg tgccgcagat
     5161 tttcatcggc gaaacgcacg tcggcggatt taccgacctc taccgcctcc agcaggaagg
     5221 cgggctggac ggactgctga acccttaacc cccaactagg aaaacaaaat gagcgaagaa
     5281 ctgcaacccg tattcagcat cgagcgactg tatgtcaaag acttgtcttt ggaagtgccg
     5341 cacgcgccgc aaatcttttt ggaacagggc gagcctgaag tggatatgcg cgtttccacc
     5401 ggcagccaaa agctggaaga cggctactac aacgtggacg ttaccgtaac cgttaccgcc
     5461 aagctggata acgagcgcac gatgtttttg aacgaagtaa cccaaagcgg catcttccgt
     5521 ttggaaaaca tccccgaaga agatgtgcag ctgctgctgg gcgtggcgtg tccgaacatc
     5581 ctcttccctt acgcgcgcga agccgtttcc ggtacggtaa cacgtgccgg cttcccgccc
     5641 gtcctgctcg cgccgattaa ttttgaggcg atttaccaac aacagcagga agccgaagcc
     5701 gccggggctt gattcctgcc tgccgatgcc gtctgaatcc gtttcagacg gtatttttgt
     5761 tttcggataa aataggtgcg ttcaaaggaa tctgcctatg atgtcgcccg aaacccagaa
     5821 acagctcaaa atcaccgatg tttccgccaa gaagctcgac aaactcaacc tccataccgc
     5881 gtgggatttg gtgttgcacc tgccgctgcg ttacgaggac gagacgcaca ttatgccgat
     5941 taaggacgcg ccgattggcg tgccgtgcca ggtcgagggc gaggttatcc atcaggaagt
     6001 aacgttcaaa ccgcgcaagc agttgattgt ccaaatcgcc gacggttccg gcagcgtcct
     6061 ttttctgcgc ttcatccact tttacgccag ccatcagaaa cagacggcgg tcggcaaacg
     6121 catccgcgcc gtgggcgaaa tcaaacacgg cttttacggc gacgagatga ttcatcccaa
     6181 aatccgcgat gccgagggcg gcggtttggc ggaaagcctc acgccggttt acccgaccgt
     6241 aaacggtttg aaccagccca ctttgcgccg tattattcag acggcgttgg acgttacgcc
     6301 gctgcacgac acgctgcccg atgccctgct gtgccgtctg aagctgccac acctcgccga
     6361 aagcctgcgt cttttgcatt cgccgccgcc gagtttcacg attcatcagc tttcagacgg
     6421 cacgctgcct gcatggcaac ggctcaaatt cgatgaactt ttggcgcaac agctttcgat
     6481 gcgcttggcg cgacagaagc gtatcggcgg cacggcggcg gcattgggcg gcgacggcac
     6541 attgacccaa gccctgcgcc aagccctgcc gtttgccctg accgatgcgc aagaaaaagt
     6601 tgtttccgaa atctgccgcg atatggcgca aacctacccg atgcaccgcc tgctgcaagg
     6661 cgatgtcggc agcggcaaaa ccattgtcgc cgccttgtcc gcgctgaccg ccatcgaatc
     6721 cggcacgcag gtggctgtaa tggcgcccac tgaaatcctt gccgaacaac attttattaa
     6781 atttaaacaa tggctcgaac ctttgggtat tgaagttgtc cgcctttttg gcagtttgcg
     6841 taaaaaagcc aaagacgaag ccaaagccaa actcgccgac ggcagcgtca aaatcgccat
     6901 cggcacgcac gccctgtttt cagacggcgt ggcgtttcac aatttgggct tgaccattgt
     6961 ggacgaacag caccgtttcg gcgttgccca acgcctcgcc ctcaaaaaca aagggcgtga
     7021 agtccatcag ctgatgatgt ccgccacgcc catcccgcgc acgcttgcga tgagtttttt
     7081 cgccgatttg gacgtgtccg tcatcgacga attgccgccc gggcgcaccc cgattaaaac
     7141 gcgcctcgtc aacaacgtcc gccgcgccga agtcgaaggc ttcgtcctcg gcacttgccg
     7201 aaaagggcgg caggcgtatt gggtctgccc attgattgaa gaaagcgaaa ccctgcaact
     7261 gcaaaccgcc gccgaaaccc tcgcccggct tcagacggca ttgcccgaac tcaatatcgg
     7321 actggtacac gggcgcatga aggccgccga aaaggccgaa gtcatggcgg aatttgccgc
     7381 aggccgtctg aacgttttgg ttgccaccac cgtcatcgaa gtcggcgtag atgtgcccaa
     7441 tgccgccctg atggtcatcg aacacgccga gcgcatgggc ttggcgcagc tgcaccaatt
     7501 acgcggtcgg gtcgggcgcg gcgcggcaga aagcgtgtgc gtcctcctgt ttgccgaacc
     7561 cttgggcgaa ctcgccaaag cgcggctgaa agtcatctac gaacacaccg acggctttga
     7621 aatcgcccgc caagacctca atatccgcgg cccgggcgag tttctcggcg cgcgccaaag
     7681 cggcgtgcct atgctgcgtt tcgccaaact cgaagaagac ctgcaccttt tggaacaggc
     7741 gcgcgaaacc gccccgatgc tgattgagca aaaccccgaa atcgtcgaag cgcatttggc
     7801 aaggtggctt tccggcaggg aagggtattt gggcgtgtga gcaaaatgcc gtctgaaata
     7861 ttagaaaaaa aagtgtaacg gaaatctgat
//
LOCUS       DAWFLT010000091         7478 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWFLT010000091 DAWFLT010000000
VERSION     DAWFLT010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7478
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.091"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..434
                     /locus_tag="WGR74_001845"
     CDS             <1..434
                     /locus_tag="WGR74_001845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906208.1"
                     /translation="KNIPLKDFDEVCEKWEKSFKENGKEIEYNQKYSRIYGEVRGAGF
                     NGDIGEKLHRCAVMEWAENETETFAKFLSLPTAETQTQVFSCFRPKYLLILPKYPLNP
                     ARIPDNQASGLLFRRQRAHLACWPLSTGSNTSPSGGFAHSL"
     gene            complement(346..1353)
                     /locus_tag="WGR74_001846"
     CDS             complement(346..1353)
                     /locus_tag="WGR74_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0906209.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSELLKLINRQLTEKGLKVEKASAAVVDATIIQTAGSKQRQAIE
                     VDEEGQISGQTTPSKDKDARWIKKNGLYKLGYKQHTRTDAEGYIEKLHITPANAHECK
                     HLSPLLEGLPKGTTVYADKGYDSAENRQHLEERQLLDGIMRKACRNRPLTEAQTKRNR
                     YLSKTRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAA
                     A"
     gene            complement(1623..3710)
                     /locus_tag="WGR74_001847"
     CDS             complement(1623..3710)
                     /locus_tag="WGR74_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEZ0906210.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGIALELFPNTHFEEKKETKKIKILSLL
                     RGISGLKRSLIQMLILAISLEVFALVSPFFMQWVIDHVIVTADKNLLLTLTLGFGLLA
                     ILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSIDH
                     IQETLTSTFFVLVLNSLMAVFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLRNA
                     TEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLSAL
                     FEFSNKLLFSMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYIQI
                     KMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFENI
                     NLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGLSG
                     IVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDMGN
                     ILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAHRQ
                     ETIQSADKILNLG"
     gene            complement(3713..4978)
                     /locus_tag="WGR74_001848"
     CDS             complement(3713..4978)
                     /locus_tag="WGR74_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ0906211.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLQTMGVVRVYSPDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRPRLESLKLEQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKPKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            complement(4945..5159)
                     /locus_tag="WGR74_001849"
                     /pseudo
     CDS             complement(4945..5159)
                     /locus_tag="WGR74_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6201..6437)
                     /locus_tag="WGR74_001850"
     CDS             complement(6201..6437)
                     /locus_tag="WGR74_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ0906212.1"
                     /translation="MKELHTSELVEVSGGKFHISAQGGGNLGKKDMVAVGKIGASYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGFSKKW"
BASE COUNT         2247 a         1392 c         1431 g         2408 t
ORIGIN      
        1 taaaaaatat ccctttgaaa gattttgatg aagtttgtga aaagtgggag aaatccttta
       61 aagaaaatgg aaaagaaatt gaatataatc aaaaatattc aagaatttat ggtgaagttc
      121 gtggagctgg ctttaatggc gatatcggag aaaagctaca tcgttgtgcc gtgatggaat
      181 gggctgaaaa tgagaccgag acctttgcaa aattcctttc cctcccgaca gccgaaaccc
      241 aaacacaggt tttcagctgt tttcgcccca aatatctcct aattctaccc aaataccccc
      301 ttaatcctgc ccggatacct gataatcagg catccgggct gctttttagg cggcagcggg
      361 cgcacttagc ctgttggccg ctttcaacag gttcaaacac atcgccttca ggtggctttg
      421 cgcactcact ttaatcagtc cgaaataggc tgcccgcgca tagcggaatt tacggtgcag
      481 cgtaccgaag ctctgttcga ccacataacg ggtcttcgac aaataccggt tgcgtttggt
      541 ttgcgcttcc gtcagcggac ggttgcggca ggctttgcgc ataatgccgt ccaacaactg
      601 acgctcttcc agatgttgcc ggttttccgc actgtcgtag cctttatcgg catagacggt
      661 cgtacctttg ggcagacctt ccaacaacgg cgacaggtgt ttgcactcat gggcattggc
      721 gggggtaatg tgcagtttct cgatatagcc ttccgcatcg gtacgggtat gttgtttgta
      781 accgagtttg tagaggccgt ttttctttat ccaacgggca tctttgtcct tactcggtgt
      841 ggtttggccg ctgatttgtc cttcttcatc gacttctatg gcctgacgct gtttgctgcc
      901 ggcggtctga ataatggtgg cgtcaacgac ggcggcggat gctttctcta cttttaggcc
      961 tttttcggtc agttgtcggt tgatcagttt gagcaattcg gacagggtgt cgtcttgcgc
     1021 cagccagttg cggtagcggc ataaggtgct gtaatcgggg atgctcagtt cgtcaaaacg
     1081 acaaaacagg ttgaagtcga tgcgggtgat gaggctgtgt tcgagttcgg gatcggagag
     1141 gctgtgccat tgtccgagca ggacggcttt gaacatggac aacaggggat aggcgggacg
     1201 gccgcggtgg tctctgaggt aacgggttct ttgacggttc aggtattgtt cgatcggttg
     1261 ccaatcaatc acctggtcca acttcaatag tgggaaacgg tcgatgtgtt tggcaatcat
     1321 ggcttgggcg gtttgttgga agaaggtgct catgagaaat cccctaaatg tcatggtggg
     1381 aatttaggga attttgggga attttgcaaa ggtctcagac cgtgaaatta gcccaaaaat
     1441 gggaacaaga gcagaaaaaa caacaaattc aacagaaaaa ggaaactgaa aaatcgccaa
     1501 aacacaaagc cagtcgtgat gattgggaaa tggaacgtta aaccgctatt ttcatgtatt
     1561 ctattaaatt atttgaaaaa agacttcact ttttgttaaa gaaaaatgaa gtcttgttct
     1621 gttcaaccta aattcagaat tttatctgcc gattgaattg tttcttggcg gtgtgcaacc
     1681 attattttca taataccaag actttttagg ttatggttaa ttttttgttc attttctaca
     1741 tctaaatggc tacttgcttc gtctaaaaat agaattttgg gtcgtttata caatgcacga
     1801 gccaagataa ctctctgctt ttgtccacct gataagatat ttcccatatc gccaatcaag
     1861 gtctcatagc ccattggcat tttaagtata tcgtcatgta tttgtgccat ttttgcacat
     1921 tgttcaatga gctccatatt tgggctttca tcaaaaaatg aaatattttc cccaatagaa
     1981 cctgcaaaaa ggacatcatc ttggcgaaca atcccgctca atcccctaat aaaggatgga
     2041 gaaacttgat atatatcatg cccattaata ctaactgtac cagtttcagg ttttaggcta
     2101 cctgttaaaa tgtttaacaa agtggacttc ccccgaccag attgtcctgt taaaacaact
     2161 gcttcattat ctttaaattc caaattaatg ttttcaaaaa gatatggctc attatcagca
     2221 tatctgaatg agacgttttc aactttaaga accagttgtt cattatctaa tttaggtata
     2281 tgattatact taataatttc agtttctgtt tcatttaaag taatgtcagc caaacgttca
     2341 gcatgaagcc ctaacatttt gatttggatg tattggtcaa cgagagaagc tgttctgctt
     2401 tcaaattgcc ctttataagc caaaaaagcc atcagaacac cgactgtaaa tgaaccatct
     2461 aaaattgcgc ttgcaccaag ataaattatg ataatatttt ccatgctaaa caacagttta
     2521 tttgaaaatt caaataaagc agagagttta tctgttgtca gcttggtatt gactgtattc
     2581 acaaataggc tcatccaagt gccatgtctt tgataatgtt tatcaaataa tttaactgat
     2641 tggataccac gaatggtttc catgaaatat gagttttgtt tggcttcatg aacaatattt
     2701 tcttctgttg catttcttaa tgggtaatat gcaagccaac gaattagtat gtacaaaaca
     2761 agtgttaaaa gaacaatcag cgataattga gtgctgtaaa ttgtcattaa cacgaaagta
     2821 aaaacagcca ttaagctatt taaaactaaa acaaaaaaag tagaagttag tgtttcttgg
     2881 atatgatcta ttgaaccaaa tcttgaaatc acatctccta aatgtcgttt actgaaatag
     2941 tcattaggta agtcaagtaa ccttttaaat atattggctt tccattgtaa attaagagtt
     3001 gtagataggt gcatacctac ccatgcttgt aacaggctaa ttaactgttg caggatagcc
     3061 agtaaaccaa atcccaaagt aagggtcagt aataaatttt tatcagcagt tacaatgaca
     3121 tggtctatta cccattgcat aaagaatgga ctaaccaatg caaagacttc caaagaaata
     3181 gctaatataa gcatttgaat taaagagcgt tttaagcctg aaatccccct taatagagat
     3241 aatattttga ttttctttgt ttcttttttc tcttcaaaat gggtattggg gaataattct
     3301 agggcaatcc ctgtgaattt ttgtgaaact tcgtccattt tgatttttcg cataccgaca
     3361 gcagggtcca taatgacgat actgtctttg gaaatggaac aaagtacaac aaaatggttt
     3421 aagttccaat ggagaatgca gggtagttgt aaatttgaca gctcatctaa ctctaaacgc
     3481 aaagctcgtg gcgttaaatt catttcattg ccaaatctca tgatgtctgc aagatttgcg
     3541 ccctttaatg acagggtgta tttttggcgt aacgttctta aatcagtgtg aaagccataa
     3601 taggacaaga tggatgtcag gcatgctaaa ccacattcag caacttctgt ttgcagaatg
     3661 acaggtagct ttttgttaaa tccaaaggac agtcttgata aataatccat ttttaattga
     3721 tttttcctga aatgctgtaa agtgggtcaa gtacccattc gtacaatttt tttcgttcat
     3781 ggagaatatc tgcttctaaa atcatgccaa tttgaagcgg cttgttttct ccgtatgctt
     3841 taatcgtttg tttttccaat ttaactttca caagataggc aggttcattt aataaggttg
     3901 ggttagtgaa aatgatacct aaacctgata gctcttgttt accgagagca gttctggcaa
     3961 ctgaaataat ttctcctgtg gcatgtccaa atttttggta agggtacgct tggtaacgta
     4021 aaacaacttt atctttcggt ttaataaaac caacagcttt actgggtatg taaagattgg
     4081 cgaccaattc agtttgttca gggacaatgc ttaacagcaa tttagacggt tcaacttgtt
     4141 gccctatatc aacattaatt gttgatatat aacctgattt actagctcgt atggtttgtt
     4201 cggatttcaa atcaaaatcc aaaatttctt ggttcatttc cgtaatcgca cggttgagtt
     4261 ggctcaattc ggttttatgg cgttcaggca ggctgcttaa tgtaattttc tgttcatcaa
     4321 gttccctgat tgcattattt tgttcaagtt ttaggctctc caaacgtggg cgttgttcca
     4381 ataaatggct ttcggcggtc atcttatctt gttgggatac tgcgccttga ctggctaaaa
     4441 acttgttctt gttaagggtt ttttccgcta aacgaatttg acgattttgc cctgtaattt
     4501 gctgtttaat attctctaat tgattgttta aacgatgaat gttgttatga acattttttt
     4561 gctcattttg atgaatgcgc tttaaacgtt ccaattcttg taatgccaaa gtttttttaa
     4621 ggttggcttc tgctgccaat ttggcttgta cgtttccttt ttcgccaaaa cgcgatgtgg
     4681 aaagtttgaa caatggttcg ccagctttga caaagttacc atcttcaaca aatttatgcg
     4741 taatcgtgcc gatatcggga gagtaaacac gaaccacccc catagtttga agtaattgac
     4801 cttcaacggt tgttttattg gtatagctac caaaaatcaa aaagataatg atacacagag
     4861 caatgagaaa agcgcaaaaa gtcagaaata aaaaagagaa tggacgggtc aagataacct
     4921 gacctgtcca cttattttgt tgggctacaa agacttcttt tcggaataag tgggacattt
     4981 attattattt cttaaaaaaa tggatagaaa aacaccaata attaaatact tatgcctaat
     5041 aacataaatt tgctattgat tgatgtttct attttaatta cattaaattt aaccaataat
     5101 ccagaaatgg ttgcagctaa aatgcaaaag acagtggcgt aaatggtttc agtattcata
     5161 atttttctct ttcaaaattg ttcaatctaa atagaaaaag gtaacttcaa tacatattgc
     5221 tgtaactgaa attacctttt ccttaatgat gtaattatct tatggcttca tcatgccacg
     5281 accgatattg tatccgccag cagcagagaa aataccagct ccaattgcgc caacccagcc
     5341 tactggtcct gtccacttgt tccgttgagc tacaaagact tctggtctga aaaatgagtg
     5401 attgttagac atgtgtactt atttatgttt ctttgattgg gataaatttg taaaaacaaa
     5461 aaaaaaggca agttgttatt atagaagaac ttaaaatgct taacatattc attgaggttt
     5521 gtaccacata atgagatacg agataatttg ttgggagtaa aatcaaaaaa aagattaaat
     5581 atttaaaata atcactatat tttttcatta tttgttcctt tttgtattta agttcttatg
     5641 aaaaactagg gtattttgaa tagaaaatat caaaataccc tagttttatt aaggaatatt
     5701 accaccaaca agtgcttcca tattgtacag caccagtgat tgcaccacca atagctccga
     5761 atttcgcacc tggaatagca ccaataccac ctgcaaatga accaacaatt gccccaccag
     5821 ctgctccacc taatgcacta ccaatggtat tttttgagaa atcacgccag ttacaagcag
     5881 caccagaaac ttgttaaatt tcattcaaag tcaaaacttg cataatattc acctttgtta
     5941 agtgtggttg agaaaaaagt aaattttcat cgttactcat tatttgcaat catgcatata
     6001 ctgtaaccaa gcactatatt cagttggatc atagtttttc ctactgggtt gtctgatatg
     6061 actattggca ctattggcac aattgggatt agcaacttta ttgtgagtac ctaatggatt
     6121 gatatttcct gtaaagtggc tattgcatgc gcttaatgag cataaattta ttaatgtcag
     6181 aaaggataaa acaaaaaatc ttaccatttt ttactaaaac caaaagtagg tttcgaaaac
     6241 tgtacaccaa gaccttgtac atatccaaat tgcttgctaa cagaaaactc tactccacta
     6301 ttgttagggg aataggaagc accaatttta ccaacagcaa ccatatcttt tttacctagg
     6361 ttgccgccac cctgtgcaga gatatggaat ttgccacctg acacttcaac taattcagag
     6421 gtgtgtaact ctttcatgat tttctcctgt aaagaagtat ggttggaaat gtaaatcaga
     6481 attaaccttt cttaagttta atttaatact tttttttgca agtattttat tttaaattaa
     6541 atcaatattt taaaaatatt tacttgtata ctgaaaaaga aactcctaat ggtggagtgg
     6601 ggcttttgtt taagtaagca ttgaaaaaat ttaaatttcc cattatgatt aacttaatgc
     6661 aagagctttt tcaggacgta caaaccagcg aacgggcgtt ctgatgtggg caggttggtt
     6721 gtcgctaacc aatttcagta gctcaatcgt cagattggta tccgacccta tcaagctaag
     6781 gagaggctgc ggctactaca caaagcacct gaaattattg ggtgcttttg tctttttatg
     6841 gcattgtacc aatttgtgta ccacgagggc ttgttcgccc aaacgcggta acaactgaat
     6901 acacactttc cccctcaact tctttgcgtt ctaaaacgac tgtcccaatc cgagaataga
     6961 caatcatagg acgatgattg ccccttacat cactaaattc actaaaaatt tttgcgcctg
     7021 attgaaaaat catttccaca taaaagggtt acacctgttt catcgtggct taatccaaac
     7081 cgcgttaaat ctgctttgtg ttctgccaaa atgtgaatca caccaaagcc gtgattaacc
     7141 tttttgtgtt cgccgtgttt taaaacaatt tgacctgccg agaagtctgc tctattgttc
     7201 ggacaatagg gggaatatac caattacttt aactacatca ttttttgaca tttttcttgc
     7261 ctttcaattt aaaatccaaa atcatactgc cataatttag caatccaaaa aaatttaggc
     7321 agcagtcatg gtttcgtatt ttagaaaacg aaacttggtt tggtgttttg aagcatttca
     7381 gtacacaaac atggaatttt aaattgtctt gtgttgcctt gatttaggat gttccagttt
     7441 tttaataaat agtttggggc tgtcctagat aactagga
//
LOCUS       DAWFLT010000092         7360 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWFLT010000092 DAWFLT010000000
VERSION     DAWFLT010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.092"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(93..452)
                     /gene="rnpB"
                     /locus_tag="WGR74_001851"
     ncRNA           complement(93..452)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WGR74_001851"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(531..1634)
                     /gene="prfB"
                     /locus_tag="WGR74_001852"
     CDS             complement(531..1634)
                     /gene="prfB"
                     /locus_tag="WGR74_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ0906213.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIATGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1737..2630)
                     /locus_tag="WGR74_001853"
     CDS             complement(1737..2630)
                     /locus_tag="WGR74_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ0906214.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIQL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2972..3421
                     /locus_tag="WGR74_001854"
     CDS             2972..3421
                     /locus_tag="WGR74_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0906215.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3495..3803
                     /locus_tag="WGR74_001855"
     CDS             3495..3803
                     /locus_tag="WGR74_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906216.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3919..4761
                     /locus_tag="WGR74_001856"
     CDS             3919..4761
                     /locus_tag="WGR74_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ0906217.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            4751..5497
                     /locus_tag="WGR74_001857"
     CDS             4751..5497
                     /locus_tag="WGR74_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ0906218.1"
                     /translation="MQPEISAQYQHRFAQAIREGEAADGLPQERLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLLDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5519..6106
                     /locus_tag="WGR74_001858"
     CDS             5519..6106
                     /locus_tag="WGR74_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ0906219.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTALDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6112..6291
                     /locus_tag="WGR74_001859"
     CDS             6112..6291
                     /locus_tag="WGR74_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ0906220.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6532..6972
                     /locus_tag="WGR74_001860"
     CDS             6532..6972
                     /locus_tag="WGR74_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906221.1"
                     /translation="MIKETLMRPIFLSFVLFPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1886 a         1950 c         1669 g         1855 t
ORIGIN      
        1 ggtttttgtt aatccactat aaaaacacaa cctaaataaa aatgccgtct gaaccatatt
       61 tcaggtttca gacggcattt gcctgtcgga tgcacaccgg acaggcggta agccgggttc
      121 tgtctcggac agtcattcct ctaggcatac cgttgccggt atgctcaagc aacctacccg
      181 aacgctcggc gggcagcgtc attgcgttct gtttggtctt gctccgaatg gggtttggcc
      241 tgccgcatat tgttaccaaa tgcgcggtgc gcccttaccg caccttttca cccttgcctg
      301 tgctgccaaa gcagccatcg gcggttttgc tttctgttcc actttccgtc gcgttaccgc
      361 gcccggccgt taaccggcat tctaccctgc ggagcccgga ctttcctccc cgtatgcctt
      421 acgcgatacg cggcgactgt ctgcccgtcc cgtgtgcggc gcggattata acacgaaacg
      481 caaaaatgcc gtctgaaacg gtacaggttt cagacggcat acagcctaaa ctacacgccc
      541 tgtttcaggc tggcttcgat aaagccgtcc aagtcgccgt ccaatacggc tttggtgttg
      601 ccgacttcgt agcctgtgcg caagtcttta atgcgtgagg agtccaagac gtacgaacgg
      661 atttggctgc cccaacctac atcggattta ccttcttcca acgcctgttt ctcttcgttg
      721 cgtttgcgca tttccaattc atacagtttg gacttcaaca tttccatcgc ggcggctttg
      781 ttggcgtgtt gcgaacggtc gttttgacat tgcaccacaa tccccgtcgg ctcgtgggta
      841 atacgcacgg cggagtcggt tttgttgatg tgctgaccgc ccgcgcccga tgcgcgatag
      901 gtgtcgatac gcaaatcggc ggggttgatt tcgatttcaa tggaatcatc gatttcaggg
      961 taaacgaaca cggaggcaaa cgaagtatgg cgtttgttgt tcgagtcaaa cggtgaatag
     1021 cgcaccaagc ggtgaacgcc ggtttcggta cgcagcaaac cataggcgta ttcgccttcc
     1081 actcggatag tcgcgcggtt gatgcccgcg atttcgccgt cgtcttcttc gaggatttcg
     1141 attttgaagc ctttgcgctc ggcgtagcgg ctgtacatac ggaacagcat acccgcccag
     1201 tcttccgctt ccgtaccgcc cgcgcctgcg gtgatgtcga taaagcagtt gttcgggtcg
     1261 gcgggctggt tgaacatccg tttgaactcc aaatccgcca tctgtttttc cagccccgcc
     1321 acatcttcct gcacggcggc aaaaccttct tcgtcgtttt cttcgacggt catttcaatc
     1381 agcatgcggt tgtcttcgat gcccgtcgcg atgttgtcga gcgtcaacac gatgccttcg
     1441 aggattttgc gctctttgcc gatttcttgg gcgcgtttcg ggtcgttcca aagttcgggg
     1501 tcttcggaaa gaccgataac ttcttccaat cggtctttct taccctgata atccatataa
     1561 actcggatgt cttcgctgcg cttttccaaa tcattcaggg tattgttgag ctggttgatt
     1621 acttcggctt ccatgattct tttgttcttt caaaatttta ggggcgtatt gtacgggatt
     1681 cgggtatttt tttctatgga taaagccttc tagaaacacg ttcggacggc atagcgtcaa
     1741 taacggtatg ccgccagttt gcgtttgatt tcaggcaatg cggcacgtgc tgcctcctca
     1801 cccaactgga tggcacgttt tttctgatcg aatccgccga ctgcacccaa atccaaaacc
     1861 tgcggtttga taaccacatc cgcctgcccc aactcatttt gcaacgcgga aacgctcatt
     1921 acgttcagcg tctgatcgag ataagagaag aagccttggc tgatgttttt gcccggacgg
     1981 gcggaaatat cgacggcaat cacgaaattc gccccctgcc gccgggcggc actgacgggc
     2041 acgggctgcg acagaccgcc gtcaacatat gtatgcctgc cgatgataac gggttggaac
     2101 acattgggaa tggcggcgga agcgcgcaca gcctgcccgg cattcccttg attgaaagcg
     2161 acggccttgc cggtttcaaa atcagtagca acagcggcaa atttgatggg aaactgctga
     2221 atctgcctgc cgccgacttt tcggttgatg taattttgca gcttttcgcc tttgataaaa
     2281 ccactggtgg acaaggttaa atcgactaaa tcggttttgc ctaaaatttc ggcttccaat
     2341 tcgaggcggt cgggcgacat acccgatgca aaaaggctgc cgacaatcga acctgccgat
     2401 gtgccggtaa ccaccttcac aggaataccg ttttctttca aaaccttaat aatacctaca
     2461 tgggcaaatc ctttagatgc gccgccaccg agtgccaaac cgaccactgc ggcgggtttg
     2521 gcggtttgca ccggcttgcg ggcggcatta tttcccgccg tgccgcaggc ggcaagcaac
     2581 gcggcggcgg cgattgccaa aagcggtctg atttttgaaa acgttaccat attttccatt
     2641 cctttatata tcgcaccccg tcaaaaagag ggattgcttt tcttaacaac cccctttgac
     2701 agccaagcaa atgggggctt tgttaagtca tcatcaaaat taatatttct ttttttcctt
     2761 tacggaaatt atatttgaag gcatactatc caaggtggga attatctcac aacaccgccg
     2821 ttatccaaat atcccgcctt tttcccttcc tttccatcaa aatactttct ttttatattc
     2881 attaacttgt taaatcattg gctgccgggt gtcagttttt ccgacaaaat ccgtctaatg
     2941 gggtatcaac agaaccaaaa caggaacact tatgaaaatc ggaacaactt ggcagacggc
     3001 atccgctatg ctggttttgc gtctgtttgc cgcatatgaa tttttggaat cgggtttgca
     3061 aaaatggaac ggggagaatt ggttttccga aatcaacgat cagtttccat tcccgttcaa
     3121 cttgctgccg gacgcgttaa actggaatct cgccatgtat gcggagcttt tactgcccgt
     3181 actgttgctt ttgggtttgg caacgcgtct gtcggcatta gggctgatgg tcgttaccgc
     3241 cgtcgcttgg gctgcggttc acgccggttc gggttacaat gtctgcgaca acggttataa
     3301 aatggcttta atttatatcg tggtattaat cccgctgctt ttccagggtg cgggcggatg
     3361 gtcgctggat acgctgctga aaaaacggtt ttgcccccaa tgccgtctga agcaagattg
     3421 attcagtcgt ggaatctgac tttaaacatt ccaaccttat ctcgttaact tgatattctg
     3481 aaaaggaaat agaaatgaac aaaaacattg ctgccgcact cgccggtgct ttatccctgt
     3541 ctttggccgc cggtgcagtt gctgccaaca aaccagcaag caacgcaaca ggcgttcata
     3601 aatccgccca tggctcttgc ggcgcgtcca aatctgccga aggttcgtgc ggcgcggctg
     3661 gttctaaagc aggcgaaggc aaatgcggcg agggcaaatg cggtgcgacc gtaaaaaaaa
     3721 cccacaaaca caccaaagca tctaaagcca aggccaaatc tgccgaaggc aaatgcggcg
     3781 aaggcaaatg cggttctaaa taatcccacc ccttcaaacc aagccgcgtt tttcagtaaa
     3841 atgcggcttt tttaacggca aacaaagatt ttttaacaag cacatcattc ttttgtgcca
     3901 tccgaaccgg gtaaaaatat gattcaacac gcaggcttgg gctaccgccg cgacttggcg
     3961 gaagactttc tctcgctttc cgaaaacagc ccgatatgct ttatcgaagc cgcaccggaa
     4021 aactggctga aaatgggcgg ctgggcacgc aaacagtttg accgtgtggc ggaacggctg
     4081 ccgctggcgt tgcacggatt gtctatgtcg ctgggcgggc aagcaccgct ggatactgat
     4141 ttgatagacg gcatcaaaga aatgatgcgc cgttacgatt gcacgttttt ctccgaccat
     4201 ttgagctact gccacgacgg cggtcatctt tacgatttgt tgccgctgcc ctttaccgag
     4261 gaaatggtgc atcatacggc gcggcgtatc cgcgaagtgc aagaccgttt gggctgccgc
     4321 atcgccgtgg aaaacacgtc ctactatctg cattccccgc ttgccgagat gaacgaggtc
     4381 gagttcctca acgcggttgc acgtgaggcc gattgcggca ttcatctgga cgtgaacaat
     4441 atctacgtca acgccgtcaa tcacggcctg ttgtcgccgg aggctttttt gaaaaacgtt
     4501 gacgcagatc gcgtgtgcta tatccatatt gccggacatg acgtggaaac gccggaattg
     4561 ttgattgata cacatggcgc ggcagttttg ccgactgttt gggacttgct cgaacttgcc
     4621 tatgccaagc tgccgacgat tccgcccacc ctattggaac gcgattttaa tttcccgcct
     4681 tttgccgaac tcgaagccga ggtcgccaaa attgccgact atcaaacgcg tgccggaaag
     4741 gaataccgcc gtgcagcctg aaatctccgc ccaataccaa caccgtttcg cccaagccat
     4801 acgcgagggt gaagccgccg acggactgcc gcaagagcgc ctgaacgtct atatccgcct
     4861 gatacgcaac aatatccaca gctttatcga ccgctgctat accgaaacgc ggcaatactt
     4921 tgacagcgaa gaatggagcc gtctgaaaga aggtttcgtc cgcgacgcgc acgcccaaac
     4981 gccctatttt caagaaatcc ccggcgagtt cctacaatat tgccaaagcc tgccgctttt
     5041 agacggcatt ttggcactga tggattttga atatacccaa ttgctggcag aagttgctca
     5101 aattccggat attcccgaca ttcattattc aaatgacagc aaatacacac cctcccctgc
     5161 ggcctttatc cggcaatatc gatatgatgt taccgatgat ttgcatgaag cggaaacagc
     5221 cttgttaata tggcgaaacg ccgaagatga tgtgatgtac caaacattgg acggcttcga
     5281 tatgatgctg ctggagataa tgggttcctc cgcgctttcg tttgacaccc tcgcccaaac
     5341 ccttgtcgaa tttatgcctg aggacgataa ttggaaaaat attttgcttg ggaaatggtc
     5401 aggctggact gaacaaagga ttatcatccc ctccttgtcc gccatatccg aaaatatgga
     5461 aggcaattcc ccgggccaaa accatctatc cgcataaaat taccttgttc ccgatactat
     5521 gccgctaccc gacctgaccg atgccgaatt aatagagtcg cgtaaactgc ttctgcattt
     5581 tgcgcggctt cagttgcccg accaccctga tttggctgaa gatttagtgc aggaaacatt
     5641 gctgtccgca tacagcgcag gcgacagttt tcaaggcagg gcacttgtca acagctggct
     5701 ttttgccata ttgaaaaaca aaattattga cgcattacgt caaatcggaa ggcagaggaa
     5761 agtctttacc gcactggatg acgagctact ggatgaagca tttgaaagcc atttttccca
     5821 aaacgggcat tggacgccgg aagggcagcc gcaacattgg aacactccgg aaaaatcatt
     5881 aaacaacaac gaattccaaa aaattctgca aagctgccta tacaacctgc ctgaaaacac
     5941 cgcacgggta tttaccctga aggaaatact cggtttttca tccgacgaaa tacaacaaat
     6001 gtgcggtatc agcacgtcca actaccacac cattatgcac cgcgcccgag aatcattgcg
     6061 ccaatgcctg caaatcaaat ggttcaacca agaaaacccg aagtaaacgt tatgaaaaaa
     6121 tgccgcgata tcgccctgct tctttccaaa catcaggacc gggaaaccac cccgggcgag
     6181 aagatttcca tatacacaca cctgctgttc tgtccgtatt gccgtgaata taaaagacaa
     6241 cttcaaacca tcaaaagatc actggcaaaa acaaccagaa cttcaaaata aatgccgtct
     6301 gaaaaggctt cagacggcat aagctgacgg aaacaaatca aaccgattta ctgttatcgg
     6361 cagttcatcc ataatacacg cttcaaaagc agcatattcc tccatacgga atatataaat
     6421 acgcaaaaca cgaaggctgc atcaatttgc tatatttgct ttatttacct tatttcacag
     6481 acggcgctac ccctcccgcc caacccgttc tttctgaatg agcagatttc aatgattaag
     6541 gaaaccctaa tgcgcccaat cttcctatct ttcgttttat tccctatttt gataaccgcc
     6601 tgcagcacac cggacaagtc tgcccgatgg gaaaatatcg gcacaatctc aaacggcaat
     6661 attcatacat atatcaataa agacagcgtg agaaaaaacg gaaatctgat gattttccaa
     6721 gataaaaaag ttgttaccaa tctaaaacaa gaacgttttg ccaacacccc cgcatacaag
     6781 actgccattg ccgagtggga aatccactgc aacaacaaaa cataccgctt aagttcgcta
     6841 caattgtttg atacaaaaaa cacggaaatt tccacacaaa actacacagc ctcttccctc
     6901 cgcccgatga gcatcctgtc cgggacatta accgaaaaac aatatgaaac cgtatgcgga
     6961 aaaaaactct gattgcaact tatacacaaa cttacccaca aaccttatca taaaaatgcc
     7021 gtctgaaata ctgaaatatc agcatttcgg acggcatttt gccatttctc cgaaaagtta
     7081 tcctcgaagt tatccacatt atttttttaa accggcttcc atccgaaata tagtggatta
     7141 acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacgg caaggcgagg
     7201 caacgccgta ctggtttaaa tttaatccac tatataaact cgctatacaa tttcactatc
     7261 caaacgtaaa ttgttccatt gatacacaaa actgcttacc cccataattt tgataaagca
     7321 tttcttacat tcccggctcc gtcccgtaac caacacagcg
//
LOCUS       DAWFLT010000093         7343 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWFLT010000093 DAWFLT010000000
VERSION     DAWFLT010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.093"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(314..636)
                     /locus_tag="WGR74_001861"
                     /pseudo
     CDS             complement(314..636)
                     /locus_tag="WGR74_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(623..970)
                     /locus_tag="WGR74_001862"
     CDS             complement(623..970)
                     /locus_tag="WGR74_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0906222.1"
                     /translation="MTGFNDAAGVASSPSDIKGKYVQKVEVAKGVITAQMASSNVNNE
                     IKGKKLSLWAKRQNGSVKWFCGQPVQRDKADADADADAVTADTAAANKKIDTNHSHEP
                     TSRHSRESGNDGI"
     gene            complement(1031..1333)
                     /locus_tag="WGR74_001863"
                     /pseudo
     CDS             complement(1031..1333)
                     /locus_tag="WGR74_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(1362..1691)
                     /locus_tag="WGR74_001864"
     CDS             complement(1362..1691)
                     /locus_tag="WGR74_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0906223.1"
                     /translation="MGGLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(1769..1987)
                     /locus_tag="WGR74_001865"
     CDS             complement(1769..1987)
                     /locus_tag="WGR74_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ0906224.1"
                     /translation="MLKTLTFAALHFSTAFSVTYVLTGSIGVSGAVALVEPLINTVVF
                     YFHEKAWNLYEKNKTVQQTQPFQLHRCS"
     gene            2109..3062
                     /locus_tag="WGR74_001866"
     CDS             2109..3062
                     /locus_tag="WGR74_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0906225.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCYYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHAPDVREGYVSFEDAVQTADVLSLHCPLNAQTENMIGEN
                     ELQQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTNEPPKNGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(3137..5194)
                     /gene="metG"
                     /locus_tag="WGR74_001867"
     CDS             complement(3137..5194)
                     /gene="metG"
                     /locus_tag="WGR74_001867"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ0906226.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQAE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVADSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADAVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AAKFLNLEAITWANTRETLGKHAINKYKHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EEGKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     AFYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDTGAQPGD
                     KVG"
     gene            complement(5310..7148)
                     /gene="glmS"
                     /locus_tag="WGR74_001868"
     CDS             complement(5310..7148)
                     /gene="glmS"
                     /locus_tag="WGR74_001868"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ0906227.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKDAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPTDVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGELSPVVHTIPVQLLSYHTALARGTDVDKPRNLAKSVTVE"
BASE COUNT         1459 a         1768 c         2190 g         1926 t
ORIGIN      
        1 ctagccgaat tactttattt tttgatacgc aaccggccgg ttgccgtcat tcccgcgcag
       61 gcgggaatcc agtccgcttg ttcggtacat gagagggcgg attgcgcgcc tgtcagataa
      121 accccgtgtt taaacgggcg gcaatgaggt acgcgcagag ccttgaagcg caatcgatat
      181 attattttca gccaaaacgg acgcccccgc ttgccttgca aacccttaaa aaggaagcca
      241 cccggattaa tccgagtggc cgtggaaaat cacttaccgc ttgatttatt taaaatttat
      301 ggtataattt gccttatccg gtagatgaat catcgcggca ggttgacggc aggtgcttgg
      361 tgttgatttt gtcgtcggtg ccgttggcgg tggcggcttt gacgtcgtct ttggctttgt
      421 cggcgcgcgc aaccggctgt ccgcagaacc attttaccga accgttttga cgcttggccc
      481 acagggagag ttttttgcct ttgatttcgt tgtttacgcc gcttgaaagc attgtggcgg
      541 taatgacgcc gtttgcgact ttaacttcct taacatattt gcctttgatg tcggtggggg
      601 ggatgccacg ccggcggcat cattaaatcc cgtcattccc actttcgcgg gaatgacggg
      661 atgtaggttc gtgggaatga ttggtgtcga tcttcttgtt ggcggcggcg gtgtcggcgg
      721 taacggcgtc ggcgtcggcg tcggcgtcgg ctttgtcgcg ctgaaccggc tgtccgcaga
      781 accattttac cgaaccgttt tgacgcttgg cccacaggga gagttttttg cctttgattt
      841 cattgtttac gttgcttgaa gccatttggg cggtaatgac gccttttgcg acttcaactt
      901 tctgaacata tttgcctttg atgtcggagg gggaggatgc cacgccggcg gcatcattaa
      961 atcccgtcat tcccactttc gtgggaatga cgggattaaa gtttcaaaat ttattctaaa
     1021 taactgaaac tcaacgaact agattcccgc ttttgcggga atgacgaagt ttttctgcca
     1081 tttgccgtga ttcgggcaat actcggtaac ggctgatttt ttgaaagtgt ttgaaatcgg
     1141 cgcgtggtgt ttttgtgcaa acggcagatg acgcatcgcg gcaggttgac ggcaggtgct
     1201 tggtgttgat gttgtcggcg gcggtgcgcg taaccggctg tccgcagaac cattttaccg
     1261 aaccgtcttg acgcttggcc cacagggaga gttttttgtc tttgatttcg ttgtttacgt
     1321 tgcttgaagc cattatgtca gacggtattg cccgggcagc tttattcgta cactttcagc
     1381 agctcgactt caaatatcaa agtggcgtgc gggggaatca cgccgcccgc gccgtgtgcg
     1441 ccgtagccca tttccgaagg gatggtcagc ttgcgtttgc cgccttcctt cattccgccg
     1501 aagccttcgt cccagccttt gatgacttgt ccgacaccga gcgtgatggt cagcggctgg
     1561 cggcggtcga ggctggagtc gaatttggtt ccgttttcca gccaacctgt gtaatgcacg
     1621 gtaatctctt tgcctttaac tgcttctttt ccgaagcctt cttgcaagtc ttcaataatc
     1681 aggccgccca tatttgtcct ttcattgctt gttggtcaaa acggcaaggg taacataccg
     1741 tccgtcgaag tcaaatgccg ctcaaacgtc agctgcatcg gtgcagctga aacggctgtg
     1801 tttgttggac tgttttattt ttttcgtaaa ggttccatgc tttttcatgg aaatagaaaa
     1861 cgacggtgtt gattaggggc tcgaccagcg cgaccgcccc cgatacgcct atactgcccg
     1921 tcagtacata ggttacactg aaggcggtgc tgaaatgcag tgcggcaaaa gtcagggttt
     1981 taagcatcat cctctcccga attggacatt gacggaggaa tgataaaggt tatcataagg
     2041 ttgcgcggtt taaatttgct atttgttgtt agtgtaaata aatcattttt caaatgggga
     2101 tagaaattat gaatcataaa aagatcgttg ttttggatgc ggatactttg cccggccggg
     2161 tttttcattt tgattttccg cacgagcttg cggtttacgg tacgacaggt gcggatgaaa
     2221 cggcagaacg ggtgcgcgat gcacatattg tcattactaa caaagtgatg atttctgccg
     2281 atattattgc ggctaatccg cagttggagc tgattgccgt cagtgcgacc ggtgtgaaca
     2341 atgtcgatat tggggcggcg aaggcggcgg gcgttgcggt atgcaatgtc cgcgcctacg
     2401 gaaacgaatc ggttgcggaa cacgccttta tgctgatgat tgcgttgatg cggaatttgc
     2461 ccgcctatca gcgtgatgtt gcggcaggat tatgggagaa gtcgccgttt ttctgctatt
     2521 acggtgcgcc gattcgggat ttgaacggta aaacgctggc ggttttcgga cgcggcaata
     2581 tcggacggac gcttgccgga tacgcgcagg cgttcggtat gggggtggtg tttgccgaac
     2641 acaaacacgc gcccgacgtg cgcgaaggct atgtttcctt tgaagatgcg gtacagactg
     2701 ccgacgtgtt gtcgctgcac tgtccgctga acgcccaaac tgaaaatatg ataggcgaaa
     2761 acgaattaca gcagatgaag cccggtgcgg ttttgattaa ttgcgggcgc ggcgggctgg
     2821 tggatgaaaa cgcgctgctt gccgcactca aatacgggca gatcggcggg gcgggtgtcg
     2881 atgttttgac gaatgagccg cccaaaaacg gcaatccctt gctgaatgca cgattaccca
     2941 atctgattgt tacgccgcat accgcgtggg caagtcgtga ggctttggac aggctgtttg
     3001 atatattgtt ggcgaacatt cacgcctttg tgaaaggaga ggcgcaaaac cgcgtggttt
     3061 gagcttgtcg ggatgacgga aaacaatgcc gtctgaacgc ccaaagggtt cagacggcat
     3121 tttttgagat tcctgtttaa ccgactttgt cgcccggctg cgcgcctgta tccacatcta
     3181 agagcttcag tttcccgtct gccgtggcgg cactcaaaat catgccttcg gatacgccga
     3241 attttgccat tttgcgcggg gcgaagttgg cgacggcgat gaccattctt ccgtttaact
     3301 cggcagggtt ggggtaaaac gcggcgatgc cggagaagat gatacgtttt tcaaaaccga
     3361 aatcgaggtc gaatttcaaa agtttggtgc tgccttcgac ggcttcgcag ttcaatactt
     3421 tggcgacgcg catatcgatt ttcatgaagt cgtcgaaact tgcctgttcg gcgacttttt
     3481 cgtatttgcc ctcttcggcg gcaggtgcgg ctgcggcggc gatgctttgt ttgttggctt
     3541 cgattaaatc gtccacttgt ttttgctcca ctcgttgcat taaatgtttg tatttgttga
     3601 tggcgtgttt gcccaaggtt tcgcgtgtat ttgcccaagt gatggcttcc aaattcagga
     3661 atttggcggc gtttgcggcg gtttgcggca agacgggggc gaggtaggcg gtcaacatgg
     3721 tgaaggcgtt gatgagctcg ctgcacactt cgtgcaggcg ttcgtcttga ccttcctgtt
     3781 tcgccagttc ccaaggtttg ttggcatcaa cgtattcgtt cacggcatct gccaatgcca
     3841 tgatatcgcg cagggcgcgg gcgtattcgc ggttttcgta ttgctcggcg atggtgtcgc
     3901 tttcggcggc aagttttgcc agcaatgcgc tgtcggcaac gtctttcaga cggccttcaa
     3961 agcgtttggc gataaaacct gaggcgcggg cggcgatgtt gacgtatttg ccgacgaggt
     4021 cgctgtttac gcgcgaaata aagtcttgca ggttcaaatc gatgtcttcg attttgctgt
     4081 tgagttttgc ggcgatgtag tagcgcatcc actcggggtt taaaccttgt tccagatagg
     4141 atttggcggt gataaacgtg ccgcgcgatt tggacatttt ttgtccgtct acggtcaaaa
     4201 agccgtgtgc gtacacgccg gtcggggcgc ggtggccgga gaaatgcagc atggcaggcc
     4261 agaacagggc gtggaaatag aggatgtctt tgccgatgaa gtggtacatc tcggtttggc
     4321 tgtcggcttt gaagtattcg tcaaaatcga cgccgatgcg gtcgcacagg tttttaaacg
     4381 acgccatgta gccgacgggc gcgtccagcc agacgtagaa gtatttgccc ggcgcgtcgg
     4441 ggatttcaaa accgaaatac ggcgcgtcgc gggaaatatc ccagtcggac agggtggttt
     4501 cttcaccttc gcccagccat tctttcattt tgttgagggc ttcggcttgc agatggggct
     4561 tgccgtcgtg cgggttgttg ccggaagtcc atgctttgag gaagtcggcg cattctccca
     4621 gtttgaagaa gaagtgttcg gattcgcgca attcgggttt cgcgccggaa acggcggaat
     4681 acgggttaat cagttcggtc ggggaatagg tcgtgccgca gacttcgcag ttgtcgccgt
     4741 attggtcttg ggcgtggcat ttcgggcatt cgcctttaac gaagcggtcg ggcaggaaca
     4801 tttgtttttc ggggtcgaaa agctgctcga tgacgcggct ttcgattttg ccgttggctt
     4861 tcagtgcgcg gtaaatgtct tgggaaaact gtttgttttc aggggaatgg gtgctgtaat
     4921 aattgtcgta gccgatgaaa aaaccggtaa agtcggcgag gtgttcttcg cgcactttgg
     4981 caatcatgtc ttcgggcgca atgccttgtt tttgcgcggc aagcatcacg ggcgtgccgt
     5041 gggtgtcgtc ggcgcagcag tagtggcact cgtggccgcg cagtttttga aagcgcaccc
     5101 aaacgtcggt ttggatgtgt tcgaccatgt ggccgaggtg gatgctgccg ttggcatagg
     5161 gcagggcgga ggtaacgagg attttgcgtg tcatattgtg ctttgcaaac aatgggtaaa
     5221 ggcggattat accgcaaatc aaacggggaa atgccgtctg aagcctgaaa aatcgggctt
     5281 cagacggcat ttttgccaac cggcgggatt tattcgacgg ttacggattt cgccagattg
     5341 cgcggcttgt ctacatccgt gccgcgcgcg agggcggtgt ggtaggaaag gagctgcacg
     5401 gggatggtat gcacgacggg ggacagttcg ccgacatggc gcggcgcacg gataacgtgc
     5461 acgccttcgg ttgcattaaa attgctgtcg aggtcggcaa agacgaaaag ttcgccgccg
     5521 cgtgcgccga cttcctgcat attggctttg actttgtcca acaggctgtc gttgggcgcg
     5581 atgacgacga cgggcatgtt ttcgtccacc aaggcaagcg gcccgtgctt cagttcgccg
     5641 gcaggatagg cttcggcgtg gatgtaggtg atttccttca gctttaacgc gccttcgagg
     5701 gcaatcgggt aatggatgcc gcgccctaaa aacagcgcgc tggttttctt ggcaaactgt
     5761 tgcgcccagg cggcaatttg aggttcgagg tttaaagcgt gctgcacgct gccgggaagc
     5821 tggcggagtt cttcggtgta acgtgcttca tcttcgccgg ataccaaacc gcgcactttc
     5881 gccagcgtta ccgccaaacc gaacagcgcg accagctgcg tggtaaacgc cttggtcgag
     5941 gcgacgccga tttccgcacc ggcacgggta taaagcacga ggctgctttc gcgcggcagg
     6001 gcggattcca tcacgttgca gatggagagg ctgtggcggt gtcccaaaga tttggcgtat
     6061 ttcaacgcct ccatcgtgtc cagcgtttcg ccggattggg aaatggtaat gaccagttgg
     6121 tccggatcgg caatcacgct gcggtatcgg tattcgctgg cgatttcgac gtcggtcgga
     6181 attttggcga tggattcgag ccagtatttt gcggtcagcg cggcgtaata ggacgtgccg
     6241 caggcaagga ttttgacgct gcggatgctt tcaaacacgc ttttggcgtc tttgccgaag
     6301 ttttcgggaa tgaagccgcc gtcgaggaaa acctccgccg tgtctgcaat cgcgcgcggc
     6361 tgctcatgga tttccttttg cataaagtgg ctgtacggtc ccagttccaa agaggcgagc
     6421 gagagttcgg ataccttgac tttgcgttcg gcaggcaggc cgtttttatc ggtcagcctt
     6481 ttgatgccgt ctgaagccag cagcgcgatg tcgccgtctt cgaggtacgc cacgcggcgc
     6541 gtaaaggcga tgacggcgga tacgtccgaa gcgataaagg tttcatcgtc gcccaaagcg
     6601 accaaaagcg ggcagcccat acgcgccaca actaattcat caggcttgtc ttgggcaata
     6661 accgcgatgg cgtatgcgcc gtggaaacgt ttgaccgctt cttgtaccgc ttcaaacagc
     6721 ctgccgccgt tttgcgcgta ttcgtgattg atgctgtgtg cgatgacttc ggtatccgtt
     6781 tgcgattcaa aacggtatcc caaaccttcc aaacgtttgc gttcgctttc aaagttttcg
     6841 atgatgccgt tgtgtacgac cgcaatcata ccgccgctga tgtgcgggtg ggcgttcggc
     6901 tcagtaacgc cgccgtgtgt cgcccaacgc gtatgtccga tgccgatgcc gccgctgatg
     6961 cctttttcgc gtgccgcgtc ctccataagc tgcacgcgtc cgacgcggcg cacgcgtttg
     7021 attttgccgt cggtgttgac ggcaatgcct gatgagtcat acccccggta ttcgaggcgt
     7081 ttgaggccgt cggtcagaaa atcgacgacg ttgtgatggg cgcggatggc gccgacgata
     7141 ccgcacataa ctgttcctta gtatcaggtt gaaaaaaaac aggcgcggac ggctcccgtg
     7201 ccgcaccttc cgcttcggat tataaaccgc ctagtgtcgg aaaacagcaa aatgccgtct
     7261 gaaggcttgg gcttgctcaa aaaaggaggg atttccccgc ctgaccggga tgggcgttca
     7321 gacggcatta cctgccgccg gtt
//
LOCUS       DAWFLT010000094         7336 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWFLT010000094 DAWFLT010000000
VERSION     DAWFLT010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7336
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.094"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..163
                     /locus_tag="WGR74_001869"
     CDS             <1..163
                     /locus_tag="WGR74_001869"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906228.1"
                     /translation="APIAKAMMLNQGFCEAFIVVVLMGIAAIMRKRPSFSKPPHRFRV
                     CPSSRPDLQ"
     gene            200..412
                     /locus_tag="WGR74_001870"
     CDS             200..412
                     /locus_tag="WGR74_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ0906229.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            560..2143
                     /locus_tag="WGR74_001871"
     CDS             560..2143
                     /locus_tag="WGR74_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ0906230.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTVDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLREIMYFAGRDLLEEPLEARTRKGLDALKALAEILEND
                     KGLGGGGKLPAIQKILNHNTYYVAHEYMEIFNDPIYVNGFIEWANRHRLAYIGDTDLH
                     VSFVSWMAEHTRERILALAGDDYIAKEFYSDILSDRQFRRSLLCREEVGDNIRRDESV
                     AVEVIESLNFRPARGETINFDENDTLLSGIRDVMKTGEPFKTEDVAENLARRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTNYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2227..3324
                     /locus_tag="WGR74_001872"
     CDS             2227..3324
                     /locus_tag="WGR74_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ0906231.1"
                     /translation="MNHTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEGLFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQVLFGFNATDGAFEVVPQVFLWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            3438..4553
                     /locus_tag="WGR74_001873"
     CDS             3438..4553
                     /locus_tag="WGR74_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ0906232.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     ACGQALLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4678..5133
                     /locus_tag="WGR74_001874"
     CDS             4678..5133
                     /locus_tag="WGR74_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ0906233.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5204..5680
                     /locus_tag="WGR74_001875"
     CDS             5204..5680
                     /locus_tag="WGR74_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ0906234.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            5668..6594
                     /locus_tag="WGR74_001876"
     CDS             5668..6594
                     /locus_tag="WGR74_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ0906235.1"
                     /translation="MSEMKKNKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVNPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCTTEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIRWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            6659..7216
                     /locus_tag="WGR74_001877"
     CDS             6659..7216
                     /locus_tag="WGR74_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0906236.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLAQKASDKAVANESGNGLKN
                     TVGTIAMARTTDPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMDTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
BASE COUNT         1921 a         1809 c         1917 g         1689 t
ORIGIN      
        1 tgcgccgatt gccaaggcga tgatgctcaa ccagggtttt tgcgaagcgt tcattgttgt
       61 cgtcctgatg ggaattgccg ccattatgcg caagcgtccg tctttttcca aaccgccgca
      121 ccgtttccgc gtttgcccaa gttcacgccc cgacttacaa taacgcccgt cagattgcag
      181 ttccaacgaa aggaaaatga tggaaaccct tatcctcgac atcggcggca tgagctgcgg
      241 cggctgcgtc aaaagcgtta cccggatatt ggaaggcgta aaaggcgtgg caagcgtcga
      301 agtcagtctt gaaaacaaaa gtgcgaccgt cggatacgac cccgcgcaaa ccgatgcagg
      361 ggcgttgatt gaagccgttg aagacggcgg ctatgatgcg gcgttgaaat aaagcggcaa
      421 aaatgccgtc tgaagccttc gcgcctcccc gatgccgtct gaagtacgct ccgcgccttc
      481 agacggcatt tgatttagtt gaacgggtag gatgaattgg ttaaacttgc gggcaatagt
      541 gccttcagac aaacaaaata tgccgaacaa aaagaaaaaa gccgcctttg cgcgtgctgc
      601 aacggagaac ggtgtgccgg acgtgtccaa catcaaaaat tcatatgatg atttgatgta
      661 tgaatccaaa gcattttccc aaaccgccat caacgccttg gaggctcgcg cccgcatgat
      721 gggtttgcag cccgcgcccg ccgccaatgc caaggtcttg gagctgggct gttcgatggg
      781 cggcaacatc atcacgcagg ctctttatta cccggatgcg gaatttgtcg gtatcgactt
      841 gtccggcagg caggttgcgc agggcaacgc catcattgaa aaaatgggct tgaaaaatgt
      901 gcgtctggaa gaaaaagata ttttgaccgt cgatgagtca ttcgggaagt ttgactatat
      961 catcgtccac ggcatttggt cgtgggtgcc tgacgcagtt aaagacaaaa ttttttcgat
     1021 ttgccggaac aacctgaccg aacacggcat tgcctatatt tcatacaatg tttaccccgg
     1081 ctggaaacgg caggagcagt tgcgcgaaat tatgtacttt gccggcaggg atctgcttga
     1141 agaacccttg gaagcgcgga cgcggaaagg cttggacgcg ctcaaggcgc tggcggaaat
     1201 tttggaaaac gacaaaggct tgggcggcgg cggcaaactt ccggcgattc aaaaaatatt
     1261 gaatcacaat acttattatg tcgcacacga atatatggaa atattcaacg acccgattta
     1321 cgttaacggc ttcatcgaat gggcaaaccg ccaccggctg gcgtatatcg gggataccga
     1381 tttgcacgtg tcttttgttt cttggatggc ggagcatacg cgggagcgga ttttggcatt
     1441 ggcaggggat gattatattg ccaaagaatt ttatagcgat attttatccg accgccaatt
     1501 ccgccgttcg cttttatgcc gtgaggaagt cggggacaat atcaggcgtg atgagtcggt
     1561 tgccgtcgaa gtgatagaaa gtttgaattt ccgcccggca agaggggaaa cgatcaactt
     1621 tgatgaaaac gataccctgc tttcgggcat acgcgatgtg atgaaaaccg gagagccgtt
     1681 taaaacggaa gatgttgcgg aaaatcttgc ccgccgattt cccggtctag aatttgaccg
     1741 catgaaaatc aattcccagc ttttattgca aaccattctc gggcgttttt ctgtttcatc
     1801 ggacaatgcg ggcaaaccat tttttgaaga ccataaaacc tatgtgccgg cgcgttttac
     1861 aaactatgcc gccgcctttg tggaacacgg cgcggaagcg tttgtccggc ctgccaaccg
     1921 ttacaacgaa agcacgccct cattcgggta cgggcatttg tacattatgc ggcaattatc
     1981 gcggccgacg agcaaacagg cattgattga aacggttgcc gaaaacttga acatcgtcag
     2041 caccacgccc gacggtttga cattccatcc gcctgccgaa gtgtatgtgg aagaaatatt
     2101 ggcagacttg gcagacaggc attttctcgt ttcggcggat tgacgggcaa gtcccgacgg
     2161 caatccgtcc gatatatttt cagacaggaa agacacacac actgcgaaaa gcggtacacg
     2221 acacatatga accatacatt taccttgccc gatacgcgcc cgtatcccca aaatccgatt
     2281 aaaaaccacc tgctgctcaa tgcctaccag ttggcgcata attcttccca ggcttcgcgc
     2341 aaactctcgt ccggccaact tcaaaccgaa atcaggggga tgcttgagca aaaccactat
     2401 atcaaccttt ccctcgcgct gacgatgtcg cccgatgccg gaacttatgc cgcgctgctt
     2461 tccagtgtga acgcggtact cgattgcgag aaagaaggcg aagtgcagtg gttcgccctg
     2521 ccggtcgtgc tggtgtccgg ctgcaagaaa gaacgggcaa tcgagatgaa gctgccgacg
     2581 gagggattgt ttgcctgttt gcaaaactat ccgcacctgc gcgcgttgac gcaagagacg
     2641 caatggctgc cttatcttgt acattcctcc gatttgagtg cggtcgcgcc ggatgagtgg
     2701 tggcgtgcca aacaaaatac cgaagcggcg gcgcaacact tacgccgttt cgccccgcgc
     2761 cctttgcttt tgcccgaagg gcagtccgtc cacgtcgttt acgcgctggg tttcggcagc
     2821 ggcaaagttc agacggcatt gggtcagaac cttcttcagg caggtctgcc cttgatgcag
     2881 gtatggcagg aaaatcttgc atcggaaggc gttacgctgt ttgccaatcc gctttccccg
     2941 gattctccgg tacgcgcgct ttccgacggc agccacacgc gccagcgtat ggcgatggat
     3001 gtgtttgcgg caaacgcgat acgcgccgtc cgtatgcaga gtccgcgcgt cggcgtggtc
     3061 gctgcggcaa aggcgggcgg acaggtttta ttcgggttta atgcgaccga cggcgcgttt
     3121 gaagtcgtgc cgcaggtgtt tttgtggcag ctttccttta ccgacaacat cgccgtcatc
     3181 cagcaaaatt tcctcgacct gatggcggag tgccgcgtgg aacacgttta cctgttgcac
     3241 aatcccttgg gcgaacagga aagcatcccg agctatgcgg aagcattgaa acgggaaggg
     3301 cacaatccgt ttttcagcgc atagtgattt gtagtagtga taggctttct catttatata
     3361 ataaatcaaa ataaagagca caacactttt tcattctgtg ttgtgccttg agtgaaacga
     3421 aaggatgaat tataaatatg aagattgtaa ttgcaccgga ttcgtttaag gaaagcttga
     3481 cagctcaaca ggtagctgaa gcaataaaaa gaggcttcca acaatcgata gcagatgtgg
     3541 aatgtctcct ctgtcctgtc ggtgatgggg gagaaggcac tgtagatgct atccgacatt
     3601 ctcttgacct agaagaaaaa tgtcttcaag tgacaggacc ttttggacaa aaagaagtca
     3661 tgcgctattt tcaaaaagaa caactagccc tattcgaagt tgctgatttg gttggtcttg
     3721 gaaaaatccc gctagagaaa cgaaatccat tacaaatcca aactcgtggt attggagaat
     3781 tgattcgcca cctcattagt caagaaatta aagaaatcta tattggcgtt ggcggtacgg
     3841 ccagtaatga tggaggtatt gggattgctg ctggtttagg ttatcaattt tatgatgagg
     3901 atggaaatac cttgcccgct tgcggtcaag ccttactaaa cctagcttct gtttcaacag
     3961 aaaatcgcta tgaaattcct gaagatgttc acattcgtat tttagcagat gttgtgagtc
     4021 ccttatgtgg acaccaaggt gcgacctata cgtttggcaa gcaaaaaggg ttggattcta
     4081 ctatgtttga ggccgtagat caggcaatac aagattttta tgaaaaagtc tcccctgcaa
     4141 cattaaaact taaaggagca ggagctggtg gaggcatcgc tggtggtttg tgtgcctttg
     4201 ctcaggcaag tatcgtatct ggaattgaca cctgcctgga cttgattgac tttgataaga
     4261 aagtgtcaga tgttgacttg gttatcgttg gtgaaggaag actagatcgt caaagtttag
     4321 cagggaaagc gcctattggt gtagcaaaaa gaacccctgt cggagttcct gttgtcgcta
     4381 tttgtggcag tctcgttgaa gatttgcctt ccctgccatt tgaaaatatc caagctgcct
     4441 tttctatttt ggagaaaagt gaacctttag aagatagttt gaaaaatgcc agtctctatt
     4501 tggagcatac ggcttctaat attggacact tattaaatat gcctaagatt tagccaaacc
     4561 atttcttcca gatggatgtt ttggctggtt ctgccttatg cggttacgat taaccgaccg
     4621 atgccgtctg aaagggtttc cgcttcagac ggcatcgcgc cggaacaggg aggggatatg
     4681 aaaaaaccga aaatcctttt tgtctgcctc ggcaacatct gccgttcgcc gatggcggaa
     4741 tacattttgc gccgccgcgc cgccgaagcg ggcattcccc ttgaagcgga cagcgcgggg
     4801 acatcgggct ggcacgacgg cgaagatatg caccgcgaga cggcaaagat attgaaaaaa
     4861 cacggtatcg atgcttcagg ctttaccagc cgcaaaatcc gccaaagcga tgcggcggcg
     4921 tttgactgca tcatcgcgat ggacggcaag aatttgtccg aattggaaag aaccttcggc
     4981 aggcggccgg aaaaaatatt caagctgacc gacctgatac ccgaaagcgg ttacgaccat
     5041 gtccccgatc cgtggtatac gggcgatttt gaagaaacct tcaggcttgc ggatgcgggc
     5101 tgtcgggcat tgttggaaaa gatttccaaa taaagcaatt gaatacaaat ataaaaccgc
     5161 ctcctgccgt atcggttgtt cagacggcat aaacagagaa tttatgaaaa caaatttcaa
     5221 acagaaaatt atcgaacagg cacgcagcga aggcttgcag gtaaccgctt tgcgcgagca
     5281 ggtattagat attgtcttgc agcaaagcgg cgtgattaaa gcctacaacg tcttgtcgca
     5341 gatgcagcag caaagcgagg gcgtgcttgc accgcctacc gcctaccgcg cccttgattt
     5401 ttgggcggag cagggcgttt tgcacaaagt ggcggcggtc aacggctata ttttgtgcag
     5461 ccacgcgcag cacgagtgcg acgaccattg ccacgaccac gaagaagccg aagcgcacca
     5521 cagcgcgttt attttggtct gcaccgaatg tggcacggcg gacgagcaaa ccctgagcca
     5581 cgagtgggcg gcactgcgcg caggcgttgc cgaaagcggc tttgcgctga aagaagaaca
     5641 cgttgtttta accggaatct gtaaaaaatg tcagaaatga aaaaaaacaa agtccacctg
     5701 atttcaggtt ttctgggaac aggcaaaacc accgcgctca aaagcctgat ggcgcaaaaa
     5761 gacccgaacg aaaaatgggt catcatcgtc aacgagttcg gcgagatcgg tattgacggc
     5821 gcggtattga gcgacaacgg catccctgtg gcagaaatcg ccggcggctg tttgtgttgc
     5881 accgccggcc cgcaaatggg cgtaaccgtg cagaaaatgc tgcgcgacgc caagcccgac
     5941 cgcctgatga ttgaagcaag cggactggct cacgcagcca gcgtcatcga cgaactcaaa
     6001 gccaaaccgc tggacagcct tttggaaatc ggcgccgtct ttaccgtcgt caatccgcgc
     6061 cagttcatca accccgatta cgcgcaacag gcgttgtata aagaccaaat cggcatctgc
     6121 gacgtattgg tcgccagcaa aaccgattta tgcaccaccg aacagcttgc cgaatttcac
     6181 gacaaagcgg caaaactgtt cccgcccaaa gccaaagtgg tcgaagtcca aaacgcacaa
     6241 ctcgacatcc ggtggctcga tattcccgtc atcgaaaaat cacgctaccg cctcaaagcc
     6301 ctgccggaca acaccatggg cttccagtcg caaggtttca cattccccgc cggacgcgat
     6361 ttcgacggcg aaaagctgac caactttttc aacgacctgc ccaaaatgac cgaaggactc
     6421 gtccgcgcca aaggcgtgtt tcaagtattg ggaacgtggg tgtggctcaa ctgggtagac
     6481 ggacagtggg gcgcgaacca agtctcatgg cggcgcgatt cgcgttttga attgattgcc
     6541 aaatcgtttg atgcggattt aatcgaacaa aagctcaaag acgcattgga atagcgttca
     6601 cgcgccttca gacggcatat gaaatgaaaa tgccgtctga aggtctgagg aggacgggat
     6661 gaaacccaaa ttcaaaaccg ttttaaccgc gctgcttttg gcggtttccc tgccgtctat
     6721 ggcggcaacc catgttttga tggaaaccga tatgggcaat atccgtttgg ttttggacga
     6781 atccaaagca cccaaaaccg ttgccaattt cgtgcgctat gcccgaaaag gcttttacga
     6841 caatacgatt tttcaccgcg tcatcggcgg cttcgttatc caaggcggcg gattgaccga
     6901 ggacttggca caaaaggcaa gcgataaggc cgttgccaac gaatccggca acggcttgaa
     6961 aaacactgtc ggcaccatcg ccatggcgcg gacgacagac cccgattccg ccaccagcca
     7021 attctttatc aatctggcgg acaatgattc actcaactac aaaaacggac aatacggcta
     7081 taccgttttc ggcagggtcg aaagcggaat ggacaccgtt tccaaaatcg cccgcgtcaa
     7141 aaccgccacg cgcggctttt atcaaaacgt acccgtacag cccgtcaaaa tccgtcgcgt
     7201 tgttgtcggg cagtaacacg cagacagatg ttcagacggc gtcgcccgtt tcacaaaaaa
     7261 cgccgtctga agtgaaaaaa tattttaaaa cagatagttg atattgacaa attcaaaccg
     7321 aggattttaa aatgct
//
LOCUS       DAWFLT010000095         7203 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWFLT010000095 DAWFLT010000000
VERSION     DAWFLT010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.095"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(85..954)
                     /locus_tag="WGR74_001878"
     CDS             complement(85..954)
                     /locus_tag="WGR74_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ0906237.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(1049..1966)
                     /gene="hisB"
                     /locus_tag="WGR74_001879"
     CDS             complement(1049..1966)
                     /gene="hisB"
                     /locus_tag="WGR74_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ0906238.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(1963..3060)
                     /locus_tag="WGR74_001880"
     CDS             complement(1963..3060)
                     /locus_tag="WGR74_001880"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ0906239.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            complement(3106..4395)
                     /gene="hisD"
                     /locus_tag="WGR74_001881"
     CDS             complement(3106..4395)
                     /gene="hisD"
                     /locus_tag="WGR74_001881"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ0906240.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLKAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAIDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(4392..5291)
                     /locus_tag="WGR74_001882"
     CDS             complement(4392..5291)
                     /locus_tag="WGR74_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ0906241.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            complement(5395..6048)
                     /locus_tag="WGR74_001883"
     CDS             complement(5395..6048)
                     /locus_tag="WGR74_001883"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ0906242.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            complement(6127..6780)
                     /locus_tag="WGR74_001884"
     CDS             complement(6127..6780)
                     /locus_tag="WGR74_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ0906243.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQVASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFNLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            7057..7141
                     /locus_tag="WGR74_001885"
     tRNA            7057..7141
                     /locus_tag="WGR74_001885"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7091..7093,aa:Leu,seq:caa)
BASE COUNT         1476 a         1715 c         2124 g         1888 t
ORIGIN      
        1 cataatcggg actgatttta agcgtggggt gataaaaatg ccgtctgaaa tatgttcaga
       61 cggcattgtg tttggaaaag gcaatcagtg ttctgccaat ttcaggtaaa cgccggaaac
      121 gtcctgttcg ccgtagccgg cttcgactgc tttgcggtag ctggcagcaa cggtttcgac
      181 ggcgggcagg gtgttgcctg cctgttcaag ctctttgacg gcgaggttga ggtctttgga
      241 ggcgtgtttg agggcgaagg cgggtgggaa ttcgcggttt gcccacaggg attttttggt
      301 ttggaacatg ggcgagtcca ttgccgagcc gccgatggct tcgacgatgg tgtcggtatc
      361 gatgccgaac tgccgcgcca tcagcatcgc ttcgctgtac gcttcgccga aaatgcccaa
      421 gagcgagttc aagacgagtt tcgcgcccga acctttgccg acatcgccga aatggaaggt
      481 ttttttgccg acgagggaaa atattttttg cagcgggttt aaaacggctt cgctgccgcc
      541 gaacagaatc agcagcgtgc cgttggtggc gggcccgacc gatccggaaa cgggtgcttc
      601 ggcaaactgt ccgcctgcgg cttcgacaag tgctttgacg gcgaggtttt cggtcgggga
      661 gatggtgctc atgttgacga tgattttgcc ggccaatccg tcgcggactc cgttcaggat
      721 gtcgcacacg gcggcatagt cggaaaccat caggaaaatg acgggatagt cgcggacgag
      781 ttcggcggtg ttgccgtaaa cttttgcgcc tttggcggag atgggggcag ttttgtcggg
      841 cgagcggttg tatacgccga cttcgatgcc gccgtccaag agccgcgtta ccataggcag
      901 acccatttgc cctaagccta tccagccgat ttgtgtgtat tcgtttgcgg acatgatgtt
      961 tactcctttt ctgtttgtag tgtagtgtaa tcttatctgt tccgataccg tctgaaaagc
     1021 ctgcgggtgt ttcagacggt atggtttttt atgcggtcag cgtgcctttg gtcgaggggg
     1081 tctgtcctgc catgcgcggg tcgtgttcga ctgccatacg cagggcgcgc ccgaaggctt
     1141 tgaatacggt ttccgcctga tggtgggcgt ttttgccgct gaggttgtcg atgtgcaggg
     1201 tcatcatact gtggttgacg atgccgtgga aaaattcttc aaacaaatcg acatcgaaac
     1261 gtccgattag tgcgcgggta aattcgatgt tgtacacgag tccggggcgg ccggaaaggt
     1321 cgatgacgac gcggctgagg gcttcgtcga gcgggacgta ggaatgtccg taacggcgga
     1381 tgcctttttt gtcgccgagt gcctgccgga ttgcttgtcc gagtgtgatg ccgatgtctt
     1441 cggcggtgtg gtggtcgtcg atgtgcaggt cgcctttgca gctgatgtcg atgtcaatca
     1501 tgccgtggcg ggcgatttga tcgatcatgt gttcgaggaa gggtacgccg gtatccagcc
     1561 tgcttttgcc gctgccgtcg aggttgatgg agacggtgat ttgggtttcg caggtattgc
     1621 ggttgacggt aacgctgcgg cagccggcag cagatgcggt ttcggcaatt tgggtttcgg
     1681 cagttccggt gctttctgcg gcggtttcgg ggacagtgcg ttcgcggtgt tttctgtcga
     1741 gccagccttt gggtttgccg gtgtgttttt cgagttttgc catcaggctg ggacggatgc
     1801 cgcgtgcatc tgggtcttct gcctgctttt caaggcgttg tttgagtttg tagagtgcga
     1861 cgggggtacg gtagccgcag agtttggcga gcttggacag cgaacctgct tcttgggcga
     1921 gggtcagaaa gttgtgcagt tggcgttgtg ttttagtcaa attcatagga aatccgttgg
     1981 ttggcagtaa agttggcgaa tgatgttgag aacggcatcg ttttgtgcgg ggctgcctac
     2041 ggtaatgcgc aggcagtgtt ccaaaagcgg gtgcgcgcca tgcagttttt taaccaagat
     2101 gcggttttgt ttgagcgtgt caaacaacaa atcggcatcg ggtacgcgta tggtaatgaa
     2161 gtttgcctga cttggaaagg tgttcagacg gcatattttg cccaattcgg cgaacatccg
     2221 ttcgcgttcg tttttcaggc tgtcgatgtt ggcagagatg atgccgtagt gttgcagggc
     2281 gagtttggcg gtggtcaggc tcagttggtt catattgtag ggcggcagga ttttttgcag
     2341 ttcgccgatg acttcgggac agcctgccgc atagccgata cgcagtccgg caaaaccgat
     2401 tttgctgacg gtacgcatga cgatgaggtt gggaatgctg cccgcctgcg gcaggaagct
     2461 gtcgccgttg aatgcgccgt aggcttcgtc gacgacgacg atgccgtctg aagcttcgat
     2521 gacggcttcg atttcggcac gcgtgaaaca tacgccggtg gggttgttgg ggtaggcgat
     2581 aaaggtcagg gcagggcggt gcttcctgac ggcttcgagg acggcgggca ggttgagggt
     2641 gaaatctccg ttcagtggaa cgccgacata atccatgccg tacagcgcgg cgttgtggcg
     2701 gtacatgacg aaactgggtt cggctgccaa cattgccgcg cccggtttgg cggtcagcat
     2761 cgtgatgaac tgtatcagtt cgtccgaacc gttgcccagc gcgatgtcgg cgcagtcggg
     2821 aatgtcgaac gccgaacgta atgcttcctg taaaccgctg ccggagggat tggggtaaag
     2881 atgtatgggc gcggcggcaa gccgtgcctg ccattcctgc aacagcgttt catgtccggc
     2941 aaaagggtgg acgggacttt ccatcgaatc gagtttggca aagccgggcg gaacgtcggc
     3001 aatctgatat gccgacatag cttgtatgtc gtcgcggatg aaggagcgga cggatttcat
     3061 cgtgtttcct taatggttgg aatatgcctg tacgccgttt cggcattatt tcatacggaa
     3121 ctctgccgcg cgggcgtggg cggtcaggct ttcgccgtgt gccagcacgc tggcggtttc
     3181 gcctaatttt tgcgcgccct gttccgaaac ctgaatcagg ctggagcgtt tttggaaatc
     3241 atatgttccc aaaggcgagg aaaagcgggc ggtgcggctg gtgggcaaca catggtttgg
     3301 gccggcgcag tagtcgccga ggctttcgcc ggtgtagcgt cccatgaaaa tcgcgccggc
     3361 gtggcggatt tttttcgccc attcctgcgg gttttcgact gacagttcca agtgttcggg
     3421 ggaaatgtag ttggcgattt cgcaggcttc gtccaagtct ttggcgagta tcatcgcgcc
     3481 cctgttgccg agcgaggctt cgatgatgtc gcggcgcggc atagtttcga tcaggcggtc
     3541 catagcggct tctacttcgt cgagatacgc ttgcgacgtg ccgatgagga tggcttgggc
     3601 aatttcgtcg tgttcggcct ggctgaacaa atctatcgcc acccaatcgg caggtgtcgt
     3661 gccgtcggcg atgaccagga tttcagacgg ccccgccacc atgtcgatgc cgaccacgcc
     3721 gaacacgcgg cgtttggcgg cggcgacgaa ggcgttgcct ggaccggtga ttttatcgac
     3781 ttgggggatg gtttccgtgc cgtaggcgag ggcggcaacc gcctgcgcgc cgccgacggt
     3841 gaagactttg gttacgccgg cgacgtatgc ggcggcaagt acgatgtcgt tgcgttcgcc
     3901 ttttggtgtc ggcacgacca tgatgatttc tttcacacct gcgacgtggg cgggcatggc
     3961 gttcatgatg acggaactcg gatacgccgc cttgccgccg gggacgtaaa tgccgacgcg
     4021 gtcaagcggt gtgatttgtt gtcccaacag cgtgccgtct tcatcggtgt agctccacga
     4081 ttccattttt tggcgttggt ggtagctttc gacacggcgg gcggcggtct gcaatgccgt
     4141 ctgaacgtcg ttcggaatgc gctcgaacgc cgctttcaaa tcggcttgcg tgagtattaa
     4201 atcatcgatg cttttagcgt ttgtctgatc gaatttgttg gtgtattcaa tcaaagccgc
     4261 atcgccgcgc ttttgcacgt cggcgcaaat gtcggcgacg atgcgttcgg tttcggggtt
     4321 ttgcgcggtt tcaaaagcca gcagggcttt gagtccggct tggaaatcgg gcgattgggt
     4381 gttgagtttt ttcatgattt ggttttttcc ttttttattt ttggattggg ggtcgtctga
     4441 atattctctt gttcagtttc tgtctctgtt ttgggtctga tatacgcttt ttctcccccg
     4501 ctaccaaact tcaattcggg caggcatttt tgttgcagac cgagtttggc taatacctct
     4561 tcgctacgag aggtagtgcg gaaacggtaa cgggattgtt ttctaccgtt tttggtttcc
     4621 tcaaccattg tttcaaaacc gtccacttcg tatgcactga caacagcgtt ttgcagaccg
     4681 gtgtgaacgc cgataacgta tttcactttt gaagcaacat ctttaccgat aacccagttg
     4741 cccaacgtac gcgatttgag gttggcatcg tcttggttgt cgaaaaggta gtctaattct
     4801 tcgtcagtat ctaaatcgaa agcattgtgg attttgatgg cgagaatcag cccgtcgggg
     4861 ttaatctcgt taagtgggag agaagagaat ccaaagcggc gatctagttc ttctacgcta
     4921 ataccacccg gaccatgccc ggcaatttta tttttgagtt ttttacccaa gacagattta
     4981 acaaaatgaa ttaaggcaga ttcggcggcg agtgcttcga cttcagtcag atgatagctg
     5041 atgatatagc gaccgagttt cttgcatttg gagatggctt tgagtttccg atcgataatc
     5101 tcgccggaga ctggatcttg tgaacgacta gcaacccatt catgctcgaa gatacgatta
     5161 ccgcagcctt tgccgatgta gaggattttg tcttttttca ggtcggtcag acaatagaca
     5221 taaaaccgac gctcgccgtt attcaaaacg gaaagggttg aatctgagaa tttttttatt
     5281 tttgcaacca ttttaacttc tcctatacag tttactgccg gtgatatttt cagacgacct
     5341 gtcggcggga acggataggg tcgtctgaaa acgcatcttt attcaaatga atgcttactt
     5401 cgccttcact gcgccgccga acgcctgaat aatcggctcc agcagcgcgt atttcgtttt
     5461 caaagcagcc ttgttgacca ccaggcggct ggaaatgtcg acgatgtgtt cgactgcttc
     5521 caagccgttt gccttcaagg tgttgcccgt cgaaaccaag tccacaatcg catcgctcaa
     5581 gccgaccagc ggcgcaagtt ccatcgagcc gtacagtttg ataatgtcca catggacacc
     5641 cttgccggca aaatgagatg ccgcgatttc aggatacttt gtggcaatct tcagacggca
     5701 tccgggttgc gaagctgctt cgtaatcaaa ccctttacgc acagcaacca tcatgcggca
     5761 cttggcaatc tccaaatcca aaggccggta aagccccgtg ccgccgtgtt cgatcagcac
     5821 gtctttgccc gcaatgccga agtccgccgc gccgtagcgg acataagtcg gcacatcggt
     5881 tgcgcggaca atgacaaggc ggatgttttc atggttcgtc ccgataatca gcttgcgcga
     5941 tttttcaggc tcttcagtcg gaacaatgcc ggcagcggca agcagcggca gcgtctcctc
     6001 aaaaatgcgc cccttggata aggcgatggt caaagcatta tcctgcatgg tgtggatgtc
     6061 ctttgtcggt tgaaacaata atgccgtctg aacaggcgcc gacggtgcag atggcatacg
     6121 gttttatcag agcagggtcc ttacatcggc agcaatcgtt tccggctcgc ttccgtaagg
     6181 cgagaaaatg gcaacctcac cgtttttgtc gatgagatac gcaccggaag agtggtcgac
     6241 caaatagttt tcgctgtcgt ctttttgatt gactttggca gaaaccacgc ggtattgctg
     6301 cttgatgacc ggcaggtttt ggtcgcccgt tgccgtcaga ccgataaagt ccggattgaa
     6361 ctgtttggca tacttgccga tgatttcagg cgtgtcgcgt tccggatcga tgctgacgaa
     6421 caccactttc acgtccttag cctgcccgcc caactgcttc aacgtgtcgc tgtacgtcaa
     6481 aagctctgtc gggcagacat cgggacagtg cgtaaagccg aaagacagaa tcacgacctt
     6541 gcctttcaaa tcgctcaggt tgaaaggctt gccttcgccg tcggtcagcg tgaaatcgcc
     6601 gccgatgtct tccttacgca tatccgtacc gcgcgtttgc ggctttgccg cattttccgc
     6661 agccgacgcg gatgcacttg aagaagcgac ttgcgccgca ctgttgtctt gaggtttgca
     6721 ggcggcaagc gcggcaagta cgaaaacgcc cggtaaaaag gaacgcggta cggaaaacat
     6781 gacaatctcc caagaataaa tagcaaaacc tgattcgtac ggaatttgaa tatttccatc
     6841 ttaacgcgtt tggcagattt aatgtagtat ttttttacgt tgtctgcacg gcaggaattg
     6901 ccgaatcata cagaatcttt ggagcggaaa gaacagtaag attaaaataa taaaaattct
     6961 atttaaaatc aaaaataaaa taaaaacaga taaaataatt tcaaaaaaaa ggtttacaga
     7021 atttcaaaag ccattataat gacgacttct tcaccagccc aggtggcgaa attggtagac
     7081 gcaggggact caaaatcccc cgccgcaagg tgtgtcggtt cgagtccgac cctgggcacc
     7141 acaaccgctt ttagagcggt tatttttttg tctattgctg tttaatgaaa tagataagac
     7201 taa
//
LOCUS       DAWFLT010000096         7197 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWFLT010000096 DAWFLT010000000
VERSION     DAWFLT010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.096"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            326..1771
                     /gene="zwf"
                     /locus_tag="WGR74_001886"
     CDS             326..1771
                     /gene="zwf"
                     /locus_tag="WGR74_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ0906244.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVKRFAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
     gene            2051..2746
                     /gene="pgl"
                     /locus_tag="WGR74_001887"
     CDS             2051..2746
                     /gene="pgl"
                     /locus_tag="WGR74_001887"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ0906245.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            2727..3713
                     /locus_tag="WGR74_001888"
     CDS             2727..3713
                     /locus_tag="WGR74_001888"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ0906246.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            3763..4611
                     /gene="hexR"
                     /locus_tag="WGR74_001889"
     CDS             3763..4611
                     /gene="hexR"
                     /locus_tag="WGR74_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ0906247.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            4732..6378
                     /gene="pgi"
                     /locus_tag="WGR74_001890"
     CDS             4732..6378
                     /gene="pgi"
                     /locus_tag="WGR74_001890"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0906248.1"
                     /translation="MKHFHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLINLYLQTNRK"
     gene            6392..6943
                     /locus_tag="WGR74_001891"
     CDS             6392..6943
                     /locus_tag="WGR74_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0906249.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGKTPNGIKFSELACKTAKTEARLEELH
                     NRKKALIDEMAREADKKELSKRL"
BASE COUNT         1884 a         1896 c         1900 g         1517 t
ORIGIN      
        1 aaaaggtata ccgtctgaaa gtgggaagcg ggtgttatta tagtacaaaa tttgtaattt
       61 tgttacaaaa aaaatcagac atcgaaaaga aatttgattt aaatcgcaga atctttcgat
      121 tgttgtaacg gaatagggga aacctgcccg atttttggac taggtcaaat aaattgtaaa
      181 aatgggaata gacaaaaagt gtagcggtgt gtagtattac tactcattat gccgcctgcc
      241 tttgtttccg atattgcgat gcagcgtgat ggggaaaaca ggtttcagac ggcatcgaaa
      301 aattctttcc tgacgagata atgcgatgag tacacaaaca aattttgatt tggtgttgtt
      361 cggtgcgacc ggcgatttgg caatgcgcaa acttttgccc tgcctgtatc aggcgcacgt
      421 ggccggtttg cttcatcccg aaggtcgtat tttgggtgtg agccgcagcg aattggatac
      481 cgaaggtttt ctggcgaagg tggaaaccag ttccaaaatc catgtgaaag aaaatttttc
      541 cgatgaggct tgggcatcgt ttgtcaaacg tttcgcatat ctcaaagtcg atgttacgca
      601 gccggacgat tttgccgctt tgggcgattt ggtcaaggcg cgcaaagaaa ccgacaatgt
      661 cgtcatctat ctttccaccg cgccgaaatt cttcgcgcaa acatgtgaaa accttgccaa
      721 aatcggtttg aacgccgaca acgtgcgtgt ggttttggaa aaaccgttgg gtacggattt
      781 ggcttcttcc caacaaatca ataccgatgt tgcgcgttat ttcaaagaag ggcaaatcta
      841 ccgtatcgac cattatttag gtaaagaaag cctgcaaaac ctgctggcct tgcgttttgc
      901 caatgtgatg ttcgagccgc tttggaacaa taaatacatc gaaagcgtgc agcttaccat
      961 cgccgagcag ttgggcgtgg aagaacgcgg cgagttttac gacattaccg gcgcgttgcg
     1021 cgatatggtg caaaaccact tgatgcaaat gctgtgcatg actgcgatgg aagcccccgc
     1081 cagcttggat gccgacgcgg tgcgcgatga aaaagtcaaa gtcatcaagt cattgaagcc
     1141 gctgaccgtc gaatctgtca atgaaaatgt cgtgcgcgga caatataccg ccgccaaagg
     1201 catgaacggc tatcttgaag aaatcaacgt tccgcaagac agctttaccg aaacctacgt
     1261 cgccattaaa gccgaaatcg aaaacgaacg ctggaagggc gttcccttct acctgcgtac
     1321 cggcaagcgc atggcgggca aagtggcgga aatcgttttg aacttcaaag atttgaacag
     1381 ccatattttt gaaggcagtc gcaccgcgcc caaccggctc gttatcgagt tgcaaccata
     1441 tgaatccgtg cgcctctata cgcagatgaa aaccccgggg gcaggaaata aggtcgaaac
     1501 cgtgccgctg gcaaccgatt tgggcaaagc attggaaagc cgccgcgcgg aagcttacga
     1561 gcgcctgctg ctggatgtga ttaacggcaa actcgctttg tttaaccgcc gcgacgaact
     1621 tgaagccgcg tgggaatatg tgatgccgat tttggaaaac tggacaaata acaccacgcc
     1681 gccgcacggc tacggcgcac actcgtgggg gcctgaagcc gcgcgcgaac tattggcgcg
     1741 cgacggacac aagtggcacg aagagcagta atacaataat gcgttcagac ggcatggggt
     1801 ttgaaatgcc gtctgaacgt aagtaaagca gtatagtgga ttaacaaaaa tcaggacaag
     1861 gcgacgaagc cgcagacagt acaaatagta cggcaaggcg aggcaacgct gtactggttt
     1921 aaatttaatc cactataaaa agcagtcccg atatttggta tcaaaagcat aaacaactgt
     1981 tcggccgaca tattgctcaa cccgtttcca taacggaata tgccgtctga aataaaaaaa
     2041 ggacacaaat atgtttgttt ggcacgaata cgaaaatgcg gcagaagcgg cgcagtcttt
     2101 ggctgacgca gtggcggatg ctttgcaggg cgcactggac gagaagggcg gtgcggtgtt
     2161 ggcagtttcc ggcggacgtt cgccgattgc atttttcaac gccctgtcgc aaaaagattt
     2221 ggattggaaa aacgtcggca tcaccttggc agatgaacgc atcgtgccga ccgtccacgc
     2281 cgacagcaat accggtttgg tgcgcgaata cctgttgaag aacaaagcgg aagcggcaat
     2341 gtggattcct atggtggaag acggaaaaac tgaaaccgaa ttacatcccg atgctgttgt
     2401 cgattatgca ctgaaacatt acaaacagcc cgatgttttg gttttgggta tgggaaacga
     2461 cgggcatacg gcttcgattt tcccgaaagc tccgcagttt cagacggcaa tcgacggttc
     2521 ggcaggtgtc gcgctggtgc ataccacgcc cgttaccgcg ccgcacgagc gcgtcagtat
     2581 gaccttggat gcgattgccc atacggggca tgtgtttttg gcgatacggg gcgaagagaa
     2641 aaaagccgtg ttcgaccaag ccgcacaagg cgaaaaccgc gaatatccga tcaacctcgt
     2701 tttgaaccat caaggagtga actgccatgt cttctacgcc gaataaacaa gccggatatc
     2761 cccgactggt cgccgatatc ggcgggacga atgcacgctt tgcgctggaa accgcgccgc
     2821 gcgtcattga aaaagccgcc gtgcttccgt gtaaagacta cgatacggtt accgatgcgg
     2881 tgcgtgccta tctgaatcaa agcggtgcaa cagccgtacg gcacgcggca tttgccatcg
     2941 ccaacccgat tttgggcgac tgggtgcaga tgaccaacca ccattgggcg ttttccatcg
     3001 aaaccacccg tcagactttg gggctggaca ccctcatcct tttgaacgac tttaccgcgc
     3061 aggcattggc ggtaacgcag acttcaagca aagacctgat gcaggtaggc gggcaaaagc
     3121 ctgtcgaatt tgcccccaaa gccgttatcg gccccggtac cggcctgggc gtgagcggat
     3181 tggtgcacag ccacgcaggc tgggtggctt tggcgggcga gggcgggcat accagtttcc
     3241 cgccgtttga cgatatggaa gtgctgattt ggcagtacgc caaaaacaaa tacggccatg
     3301 tttccgccga acgctttttg agcggcgcgg gcttgagctt ggtttacgag gctttggctg
     3361 caaaacagaa agccaaaccc gccaaactga tgccgtctga aatcacggaa aaggctttga
     3421 gcggcgcgtc gcctttgtgc cgtcagactt tggacatctt ctgcgccatg ctcggcacgg
     3481 ttgcttccaa cctcgccctg acgctgggcg cgcgcggcgg cgtgtacctg tgcggcggca
     3541 ttattccccg cgtgttggaa tatttcaaaa cttccccgtt ccgcagccgt ttcgagaaca
     3601 agggcaggtt tgaagcatat cttgccgcga ttcccgtgta tgtcgtcttg agcgagtttc
     3661 ccggaatttc cggtgcggct gcggctctcg acaaccattt gagaaacgtt taaccacagc
     3721 ggctccttgc agcggggctg cattatcgaa gggcatatca ttatgttaag caaaatcagc
     3781 gaatcactgg caaacctttc cggtgcggaa cgcaaagtcg ccgaatgtgc attggcggaa
     3841 cccaaatggt tcgtccatgc cgccgttgcc gaaattgccg aacgtgcgtc cgtcagccag
     3901 ccgaccgtca tccgattctg ccgcagcttg ggttataaag ggctgcccga gttcaagctc
     3961 gccttgtccg ccagcatcgg tcatgagggt atgccctatg tccacgaaga actcaacgcc
     4021 gacgacgata tggcaagcgt ggtcgagaaa gtgttgggca atgccgccgc ctcgctgttg
     4081 ggcgaacgcc gcttcctgaa agagtcggag ctggaaaacg ccattgccac gctgatgcac
     4141 gcccgtcgcg tcgagtttta cggtgtcggc aattccggca ttgtggcaca ggacgcgcag
     4201 cataaatttt tccgtttcgg catgtccacc gtcgcctatg tcgatacgca cacgcagctg
     4261 atggcggcat ctgttttgag cgatcaggat gttttggttg ccatttccaa cacgggttcg
     4321 tctatcgaac ttttggatgc ggtcagcatc gccaaagaaa acggcgcgtc tgtcatcgca
     4381 ctgacccgca acgattcgcc tctggcgcaa cttgccgact gcgtgttgag cgttgccaca
     4441 caggaaaatg ccgaactcta cacgcccatg gtttcccgcc tcttgcagct tgccgtcatc
     4501 gacattctcg ccatcggact tgccctgcgc ttgggcgatg ctgccagcct gcaattgcag
     4561 aaaagcaaaa aaagcataca caacaagcac atcgattacg acaaagattg accttcagac
     4621 ggcatcccac aaatgccgtc tgaaatgccg aacaacggtc gtcggcggct tgcggcagtt
     4681 tccggcagcc ttttcatccc acaacaaaaa acctcattca ggagcatata gatgaaacac
     4741 tttcacgact tacccgcatg gtcgaaattg tggaaccact ttgacgacag caaaacattg
     4801 catatgcgcg aaatgttcga gcaagatccg cagcgtgcgg aacgctactg gctgcaagtc
     4861 ggcggactga cgctggacta ctccaaaaac cgcatcaacg acgaaaccat gtcgcttttg
     4921 ttcgagttag cgcgtgaagc aggcgtgccg gagcggatgc ggcagatgtt ccacggcgaa
     4981 aaaatcaata ccaccgaaaa ccgcgccgtc ctgcatgtcg cccttcgcaa ccgcaccaat
     5041 tcgccgattg tggttgacgg tgaagatgtg atgcccaaag tcaaccgcgt tttgcaacgt
     5101 atgggcgaat ttgcacacga agtccgcagc ggaagctggc tgggctatac caaccaagtc
     5161 attaccgacg ttgtcaacat cggcatcggc ggatcggatt tgggtccgct gatgatgtgt
     5221 accgcgctca aacctttcgg tcatccgcgc ctcaatatgc acttcgtctc caacgtggac
     5281 ggctcgcaac tgcgcgacgt attgtccaaa gtccaccccg aaaccacgct gttcatcatc
     5341 gcctccaaaa catttaccac gcaggaaacg ctgaccaacg cgctgaccgc gcgcgaatgg
     5401 tttttgaatc atgcgggcga cgaagaagcc gttgccaaac acttcgccgc cgtttccacc
     5461 aatcaaaaag ccgtcgccga attcggcatc gacaccgcca atatgtttga attttgggat
     5521 tgggtcggcg gtcggtacag cctgtggtcc gccatcggat tgccgattat gctgtatctc
     5581 ggcgaagaaa acttcatcga aatgctcaac ggcgcgcacc tgatggacca acacttcatc
     5641 aacacaccgc tcgagcgcaa cctgcccgtc attctcgccc tcatcggcat ctggtatatc
     5701 aactactacg gcggcggcag ccacgtcatc gcgccttacg accaacattt gcaccgcctg
     5761 cccaaattca tccagcagct cgatatggaa agtaacggca aacaggttac gttggacggc
     5821 aaagcagtcg gacacgaaac ctcgccgatt atctggggcg aaacgggcat taacggccag
     5881 cacgccttct tccaactgct gcaccaaggc acgcacatta cccccatcga cctgattgcc
     5941 tcgcttgaaa aacgcagcaa cctgcccgga caccacgaaa tcctgcttgc caatgtcttc
     6001 gcccaagcag aagcctttat gcgcggcaaa acccccgacg aagtccgcgc cgaactcaaa
     6061 gcgcagggta tggatgaggc gcgcatcgaa gagctggtcc cgcacaaaac cttctccggc
     6121 aaccgcccga ccaacctcat tctcatggac aaggtcaacc cgcgcaatat gggcagcctg
     6181 attgccatgt acgaacacaa aaccttcgta caaggcatca tttggggcat caacagcttc
     6241 gaccagtggg gcgtggaact cggcaaacaa ctggctaaaa ccattttggg cgaactgacc
     6301 ggcgaaaccg ggccgcaaaa gcacgacagt tcgaccgaac gcctgatcaa cctctacctg
     6361 cagaccaacc gcaaataaaa cctgcggaaa aatgccgtct gaacgccgac cgtttcagac
     6421 ggcattttta tcgaacagga aaaccgtcgg taaacttgca gagcgtgtgc aatcccgata
     6481 tgatggtttg cataaattta aacatatatg ttccgcagct atggcactga ttaaagagcc
     6541 gttggacaaa gcgaaacaaa ggaacgaaga acttgaagcg gcagaagaag cggcggcgca
     6601 ggaggcattg ggtcgggagc aggaagtcga ccgcgtatcc gaatgggaag aacgctacaa
     6661 gctgtcgcgc agcgagttcg agcagttctg gaaaggattg cctcaaaccg tacagaataa
     6721 gctgcaagcc tcacagaaaa catggaaaag cgggatggat aaaatctgtg ccaacaatgc
     6781 gaaagctgaa ggtaaaacgc caaacggcat aaaattcagc gaactggcat gcaaaacggc
     6841 gaaaaccgaa gcacgcttgg aagagctgca caaccgtaaa aaagccctta tcgacgaaat
     6901 ggccagggaa gcggacaaga aagaactgtc aaagcggctc tgaacagcgc ggttcaggca
     6961 ctgcccgccg atattgccga aaccgtcatg cccgagtacc gcaactgaca aaacggtttg
     7021 aatgccaaat gcgccgacgg cgacgaatac ggcgtggcac aatctgactg ccgtaccaga
     7081 gaaatcaatg cgaaaaccaa agaaatccaa ggttatctga ttgactgaaa cttggatgcg
     7141 ggaatgtcgg cggcttttgc gtttttgtcg tttttatagt gaattaaatt taaaccg
//
LOCUS       DAWFLT010000097         7158 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWFLT010000097 DAWFLT010000000
VERSION     DAWFLT010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.097"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(111..1457)
                     /gene="mnmE"
                     /locus_tag="WGR74_001892"
     CDS             complement(111..1457)
                     /gene="mnmE"
                     /locus_tag="WGR74_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ0906250.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAEALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGVNDKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGEGEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1588..1743
                     /locus_tag="WGR74_001893"
     CDS             1588..1743
                     /locus_tag="WGR74_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906251.1"
                     /translation="MEKRNTYALLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1740..2171
                     /locus_tag="WGR74_001894"
     CDS             1740..2171
                     /locus_tag="WGR74_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ0906252.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2305..6654
                     /gene="app"
                     /locus_tag="WGR74_001895"
     CDS             2305..6654
                     /gene="app"
                     /locus_tag="WGR74_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ0906253.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGRNPDQHRFSYQIVKRNNYKPDNSHPYNGDYHM
                     PRLHKFVTDAEPVEMTSDMRGNTYSDKEKYPERVRIGSGHHYWRYDDDKHGDLSYSGA
                     WLIGGNTHMQGWGNNGVVSLSGDVRHANDYGPMPIAGAAGDSGSPMFIYDKTNNKWLL
                     NGVLQTGYPYSGRENGFQLIRKDWFYDDIYRGDTHTVFFEPRSNGHFSFTSNNNGTGT
                     VTETNEKVSNPKLKVQTVRLFDESLNETDKEPVYAAGGVNQYRPRLNNGENLSFIDYG
                     NGKLILSNNINQGAGGLYFEGDFTVSPENNETWQGAGVHISEDSTVTWKVNGVANDRL
                     SKIGKGTLHVQAKGENQGSISVGDGTVILDQQADDKGKKQAFSEIGLVSGRGTVQLNA
                     DNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNATTTSTVTITGNESI
                     TQPSGKNINRLNYSKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLSGGTNLNG
                     NITQTNGKLFFSGRPTPHAYNHLGSGWSKMEGIPQGEIVWDNDWINRTFKAENFHIQG
                     GQAVISRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEKTITDDKV
                     IASLTKTDISGNVSLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNGNLSLVGN
                     AQATFNQATLNGNTSASGNASFNLSNNAAQNGSLTLSDNAKANVSHSALNGNVSLADK
                     AVFHFENSRFTGQLSGSKDTALHLKDSEWTLPSGTELGNLNLDNATITLNSAYRHDAA
                     GAQTGSVSDTPRRRSRRSLLSVTPPTSVESRFNTLTVNGKLNGQGTFRFMSELFGYRS
                     DKLKLAESSEGTYTLAVNNTGNEPVSLDQLTVVEGKDNKPLSENLNFTLQNEHVDAGA
                     WRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDALIAAGRDAAEKT
                     ESVAEPARQAGGENVGIMQAEEEKKRVQADKDSALAKQREAETRPATTAFPRARRARR
                     DLPQPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFAEDRRNAVW
                     TSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTFDDGIGNS
                     ARLAHGAVFGQYGIGRFDIGISTGAGFSSGSLSDGIGGKIRRRVLHYGIQARYRAGFG
                     GFGIEPYIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHISITPY
                     LSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAKGPQLE
                     AQHSAGIKLGYRW"
     gene            complement(6970..>7158)
                     /locus_tag="WGR74_001896"
     CDS             complement(6970..>7158)
                     /locus_tag="WGR74_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0906254.1"
                     /translation="PYWFKFNPLYVVEQSFGTLHRKFRYARAAYFGLSKVSAQSHLKA
                     MCLNLLKAANRLSAPAAA"
BASE COUNT         1880 a         2088 c         1752 g         1438 t
ORIGIN      
        1 gccttgtcct gatttttgtt aatccactat aaaaccgaac ggatcggatt cccgttttta
       61 tgggataacg gaatgttcag ccggacaccg ccacggtgct ttcgatccgt ttattttccg
      121 atacaaaacc tcgaaaaaat cacgccgagc aggtcgtccg ccgtaaactc gcccgtgatt
      181 tcgccgcatg cgacctgcgc caagcgcaag tgttcggcaa acagctcgat ttgatggttg
      241 ccgcacaatg ccgccagcga caattcttcc tgcgctgctt tgagtgcgtt gacgtgccgc
      301 gtccgcgcca aaaacagccc ttcgccctcg ccctgccatc cggcctcgcg caacaacgtc
      361 cgtttcagcg cgtccaagcc gtcgccggtt ttcgccgaca gcgcgatgac ggtttccgcg
      421 cccgtaccga acccgcctgc cgcgtgtgcg tgcaaatcgg atttgctgtg gatttcgatg
      481 cgtttcaact ccggcggcaa cgcgtccaaa atcgcccgtg tcttgtcgtt cacgccttcg
      541 cgcggatcga ccaacaccag cgcgacatcg gcttcggata cggctttgcg gctgcgttcg
      601 atgccgatgc gttcgaccac gtcgtccgtc tcgcgcaaac ctgccgtatc gacaatatgc
      661 accggaacgc cgtcaatcag gatacgttcc ctgaccgcgt cgcgcgtcgt tccggcaata
      721 tcggtaacaa tcgccacttc gtcgcccgcc agcgcgttca acaggctgga tttgccgaca
      781 ttcggcgcgc cgaccaatac gacattcaga ccttcgcgca aaatcgcgcc ctgctgcgcg
      841 ttggcaagca catcatccac ggcgcggcgc aagccgtcca gtttgccgcg tgcgtctgcc
      901 gcttcgagaa aatcaatgtc ttcctcggga aaatctaacg tcgcttcgac cagcatccgc
      961 aaggtaatca agtcttcgac cagaccgtgt atccgccgcg aaaaatcgcc tttgagcgag
     1021 cgcaacgcca gacgcgccgc cgaacggctg gatgcgtcaa tcaaatccgc cacgccttcc
     1081 gcctgtgcca agtccagttt gtcgtttaaa aacgcacgct tcgtaaactc gcccggttcg
     1141 gcaaggcgcg cgcccaattc caaacagcgg ttcagcagca tatccatcac taccggcccg
     1201 ccgtgtccct gaagctcgat aacatcttcg cccgtaaaac ttgccggtgc ggcaaaaaaa
     1261 agcaaaagcc cgctgtcgat tgcctgaccg tccgcgtccg taaaatcagc ataggttgcc
     1321 acgcgcggct tgggcgtttt cccacacaaa gcctctgcca tcggcagcag gtttttcccc
     1381 gatatgcgta tcacgcccac gccgccgcgc ccgggtgcgg tagcgactgc cgcaatcgtt
     1441 ggaacgttat ccgacataaa acccccgaaa attcaaaaca gccgcgatta tagcaaatgc
     1501 cgtctgaagt ccgacggttt ggctttcaga cggtataaaa ccgcaaaaat gcttgataaa
     1561 tccgtccgcc tgacctaata taaccatatg gaaaaacgaa acacatacgc cctcctgctc
     1621 ggtataggct cgctgttggg tctgttccat cccgcaaaaa ccgccatccg ccccaatccc
     1681 gccgacgatc tcaaaaacat cggcggcgat tttcaacgcg ccatagagaa agcgcgaaaa
     1741 tgaccgaaaa cgcacaggac aaggcgcggc aggctgtcga aaccgtcgtc aaatccccgg
     1801 agcttgtcga gcaaatcctg tccgacgagt acgtgcaaat aatgatagcc cggcgtttcc
     1861 attcgggacc gttgccgccg ccgtccgact tggcgcaata caacgacatt atcagcaacg
     1921 gggcagaccg cattatggca atggcggaaa aagaacaagc cgtccggcac gaaaccatac
     1981 ggcaagacca aaccttcaac aggcgcgggc aactgtacgg cttcatcagc gtcatcctga
     2041 tactgctttt tgccgtcttc ctcgtatgga gcggctaccc cgcaaccgcc gcctcccttg
     2101 ccggcggcac agtggttgcc ttggcgggtg ctttcgtgat tggaagaagc cgagaccaag
     2161 gcaaaaatta atgacaaatc ctagggcgtg cttcatatcc gcccgaacgc cgaaccgcac
     2221 atataggcac atcccgcgcg ccgcccggaa gcggaagccg cgccctccca aacaaacccg
     2281 aatcccgtca gataaggaaa aataatgaaa acaaccgaca aacggacaac cgaaacacac
     2341 cgcaaagccc cgaaaaccgg ccgcatccgc ttctcgcctg cttacttagc catatgcctg
     2401 tcgttcggca ttcttcccca agcctgggcg ggacacactt atttcggcat caactaccaa
     2461 tactatcgcg actttgccga aaataaaggc aagtttgcag tcggggcgaa agatattgag
     2521 gtttacaaca aaaaagggga gttggtcggc aaatcaatga caaaagcccc gatgattgat
     2581 ttttctgtgg tgtcgcgtaa cggcgtggcg gcattggtgg gcgatcaata tattgtgagc
     2641 gtggcacata acggcggcta taacaacgtt gattttggtg cggaaggaag aaatcccgat
     2701 cagcaccgtt tttcttacca aattgtgaaa agaaataatt ataagcctga caattcacac
     2761 ccttacaacg gcgattacca tatgccgcgt ttgcataaat ttgtcacaga tgcagaacct
     2821 gtcgaaatga cgagtgacat gagggggaat acctattccg ataaagaaaa atatcccgag
     2881 cgtgtccgca tcggctcagg acaccactat tggcgttatg atgatgacaa acacggcgat
     2941 ttatcctact ccggcgcatg gttaattggc ggcaatacac atatgcaggg ttggggaaat
     3001 aatggcgtag ttagtttgag cggcgatgtg cgccatgcca acgactatgg ccctatgccg
     3061 attgcaggtg cggcaggcga cagcggttcg ccaatgttta tttatgacaa aacaaacaat
     3121 aaatggctgc tcaacggagt tttacaaacc ggctaccctt attccggcag ggaaaacggt
     3181 ttccagctga tacgcaaaga ttggttctac gatgacattt acagaggcga tacacatacc
     3241 gtcttttttg aaccgcgcag taacggacat ttttccttta catccaacaa caacggtacg
     3301 ggtacggtaa cagaaaccaa cgaaaaggtt tccaatccaa agcttaaagt acagacagtc
     3361 cgactgtttg acgaatcttt gaatgaaact gataaagaac cagtttacgc ggcagggggt
     3421 gttaatcagt accgtccaag gttaaacaac ggtgaaaacc tttcttttat cgattacggc
     3481 aacggcaaac tcatcttatc aaacaacatc aaccaaggcg cgggcggttt gtattttgaa
     3541 ggtgatttta cggtctcgcc tgaaaacaac gaaacgtggc aaggcgcggg cgttcatatc
     3601 agtgaagaca gtaccgttac ttggaaagta aacggcgtgg caaacgaccg cctgtccaaa
     3661 atcggcaaag gcacgctgca cgttcaagcc aaaggggaaa accaaggctc gatcagcgtg
     3721 ggcgacggta cagtcatttt ggatcagcag gcagacgata aaggcaaaaa acaagccttt
     3781 agtgaaatcg gcttggtcag cggcaggggt acggtgcaac tgaatgccga taatcagttc
     3841 aaccccgaca aactctattt cggctttcgc ggcggacgtt tggatttaaa cgggcattcg
     3901 ctttcgttcc accgtattca aaataccgat gaaggggcga tgattgtcaa tcataatgcc
     3961 acaacaacat ccaccgttac cattacaggg aatgaaagta ttacacaacc gagtggtaag
     4021 aatatcaata gacttaatta cagcaaagaa attgcctaca acggttggtt tggcgagaaa
     4081 gatacgacca aaacgaacgg gcggctcaac cttgtttacc agcccgccgc agaagaccgc
     4141 accctgctgc tttccggcgg aacaaattta aacggcaaca tcacgcaaac aaacggcaaa
     4201 ctgtttttca gcggcagacc gacaccgcac gcctacaatc atttaggaag cgggtggtca
     4261 aaaatggaag gtatcccaca aggagaaatc gtgtgggaca acgactggat caaccgcacg
     4321 tttaaagcgg aaaatttcca tattcagggc gggcaggcgg tgatttcccg caatgttgcc
     4381 aaagtggaag gcgattggca tttgagcaat cacgcccaag cagtttttgg tgtcgcaccg
     4441 catcaaagcc atacaatctg tacacgttcg gactggacgg gtctgacaaa ttgtgtcgaa
     4501 aaaaccatta ccgacgataa agtgattgct tcattgacta agaccgacat cagcggcaat
     4561 gtcagccttg ccgatcacgc tcatttaaat ctcacagggc ttgccacact caacggcaat
     4621 cttagtgcaa atggcgatac acgttataca gtcagccaca acgccaccca aaacggcaac
     4681 cttagcctcg tgggcaatgc ccaagcaaca tttaatcaag ccacattaaa cggcaacaca
     4741 tcggcttcgg gcaatgcttc atttaatcta agcaacaacg ccgcacaaaa cggcagtctg
     4801 acgctttccg acaacgctaa ggcaaacgta agccattccg cactcaacgg caatgtctcc
     4861 ctagccgata aggcagtatt ccattttgaa aacagccgct ttaccggaca actcagcggc
     4921 agcaaggata cagcattaca cttaaaagac agcgaatgga cgctgccgtc aggcacggaa
     4981 ttaggcaatt taaaccttga caacgccacc attacactca attccgccta tcgccacgat
     5041 gctgcaggcg cgcaaaccgg cagtgtgtca gacacgccgc gccgccgttc gcgccgttcc
     5101 ctattatccg ttacaccgcc aacttcggta gaatcccgtt tcaacacgct gacggtaaac
     5161 ggcaaattga acggtcaagg aacattccgc tttatgtcgg aactcttcgg ctaccgaagc
     5221 gacaaattga agctggcgga aagttccgaa ggcacttaca ccttggcggt caacaatacc
     5281 ggcaacgaac ccgtaagcct cgatcaattg acggtagtgg aagggaaaga caacaaaccg
     5341 ctgtccgaaa accttaattt caccctgcaa aacgaacacg tcgatgccgg cgcgtggcgt
     5401 taccaactca tccgcaaaga cggcgagttc cgcctgcata atccggtcaa agaacaagag
     5461 ctttccgaca aactcggcaa ggcagaagcc aaaaaacagg cggaaaaaga caacgcgcaa
     5521 agccttgacg cgctgattgc ggccgggcgc gatgccgccg aaaagacaga aagcgttgcc
     5581 gaaccggccc ggcaggcagg cggggaaaat gtcggcatta tgcaggcgga ggaagagaaa
     5641 aaacgggtgc aggcggataa agacagcgcc ttggcgaaac agcgcgaagc ggaaacccgg
     5701 ccggctacca ccgccttccc ccgcgcccgc cgcgcccgcc gggatttgcc gcaaccgcag
     5761 ccccaaccgc aacctcaacc ccaaccgcag cgcgacctga tcagccgtta tgccaatagc
     5821 ggtttgagtg aattttccgc cacgctcaac agcgttttcg ccgtacagga cgaattggac
     5881 cgcgtgtttg ccgaagaccg ccgcaacgcc gtttggacaa gcggcatccg ggacaccaaa
     5941 cactaccgtt cgcaagattt ccgcgcctac cgccaacaaa ccgacctgcg ccaaatcggt
     6001 atgcagaaaa acctcggcag cgggcgcgtc ggcatcctgt tttcgcacaa ccggaccgaa
     6061 aacaccttcg acgacggcat cggcaactcg gcacggcttg cccacggcgc cgttttcggg
     6121 caatacggca tcggcaggtt cgacatcggc atcagcacgg gcgcgggttt tagcagcggc
     6181 agtctttcag acggcatcgg aggcaaaatc cgccgccgcg tgctgcatta cggcattcag
     6241 gcacgatacc gcgccggttt cggcggattc ggcatcgaac cgtacatcgg cgcaacgcgc
     6301 tatttcgtcc aaaaagcgga ttaccgctac gaaaacgtca atatcgccac ccccggtctt
     6361 gcgttcaacc gctaccgcgc gggcattaag gcagattatt cattcaaacc ggcgcaacac
     6421 atttccatca cgccttattt gagcctgtcc tataccgatg ccgcttcggg caaagtccga
     6481 acacgcgtca ataccgccgt attggctcag gatttcggca aaacccgcag tgcggaatgg
     6541 ggcgtaaacg ccgaaatcaa aggtttcacg ctgtccctcc acgctgccgc cgccaaaggc
     6601 ccgcaactgg aagcgcaaca cagcgcgggc atcaaattag gctaccgctg gtaaccgccg
     6661 gatatgccga aaggggtctg acgatgccac cgtgcgctgt caaacccttt ttctgccgcc
     6721 gccttgtgcc tgctccattg tctgataacc gtcaaattga tttttagccc atgtttggtg
     6781 ggtcggggaa atctatatct tcgtccgtgc cgaaatagtc tgagaccttt gcaaaattcc
     6841 tttccctccc gacagccgaa acccaaacac aggttttcgt ctattttcgc cccaaatacc
     6901 tcctaattct acccaaatac ccccttaatc ctccacggac acccgataat caggcatccg
     6961 ggctgctttt taggcggcag cgggcgcact tagcctgttg gccgctttca aaaggttcaa
     7021 acacatcgcc ttcagatggc tttgtgcact cactttgctc agcccgaaat aggctgcccg
     7081 cgcatagcgg aatttacggt gcagcgtacc gaagctctgt tcgaccacat atagtggatt
     7141 aaatttaaac cagtacgg
//
LOCUS       DAWFLT010000098         6908 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWFLT010000098 DAWFLT010000000
VERSION     DAWFLT010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.098"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(1..617)
                     /locus_tag="WGR74_001897"
                     /pseudo
     CDS             complement(1..617)
                     /locus_tag="WGR74_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein FrpC"
     gene            complement(1968..2983)
                     /locus_tag="WGR74_001898"
                     /pseudo
     CDS             complement(1968..2983)
                     /locus_tag="WGR74_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
     gene            4257..6389
                     /locus_tag="WGR74_001899"
     CDS             4257..6389
                     /locus_tag="WGR74_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ0906255.1"
                     /translation="MSIVSAPLPALSALIILAHYHGIAANPADIQHEFCASAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPVKRLAMATLPALVWCDDGNHFILAKTDGGGEHAQYLIQ
                     DLTTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
     gene            6613..>6908
                     /locus_tag="WGR74_001900"
     CDS             6613..>6908
                     /locus_tag="WGR74_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0906256.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIP"
BASE COUNT         1867 a         1523 c         1445 g         2073 t
ORIGIN      
        1 ccaatgcccg caagattggc ggcttttgcc tgttcggcag tgagttccac tttgtctttg
       61 aagcggctgt gcagattgtc ggctgctaaa agtaaatccc ccatttttgc ggttgtaccg
      121 tctgttttgg tgtagctgcc ttgctgagcc aaagtgttac cgttgccgag atttttattt
      181 acatctttat aggcgagatc caaagattgg atacccaatt cttcaagggt acgcaattca
      241 ttagcttggg aaatgccgtc ctggttgaga tcctgccata cacgcaggga ttggaatgcg
      301 gcgtctgccg cgttgatgat gttgtcgccg tttgaatcca attcggccaa agccgcgtag
      361 ccgtgtttgg caaaagagcc gtctgccagt ttggtgttgt cgccgaagag ttccgcaccg
      421 ttgtcgatga tgccgttgcc gttcaaatcg cgcacaagca gaccgtcatc ggcagcaacc
      481 caacctgtgg cggtgcggat gccttgattg cggtggtcga acaatgcacc tgcaaagcct
      541 ttggcagcga cagtttctat gccgtcgccg tccaaatcta gggcaagggg gtcgtaaacg
      601 tggtattggc cgtttcgatt aagaaatagg aaatctcttg ctcctttata cccttcccat
      661 gctaaaccac ccaacccatc ttcaatccac ccccctaatt tgtctcgtaa ccactcagga
      721 gttaaaacac cagttccacc acccaataat ccgcctaaga ccgcgccacc aagagcaaac
      781 gcacctacag tcagtgcagc aggcaatccc gctaaaccaa gaccagtaac aataaaacca
      841 gcaacccatg caccaaatgc tcctcctatc tcacccaatg ctagtcctgt acctattttt
      901 gatgaagttt ttccaagatt atcatagtca ttttcattga ttgcttttgt ccatgcagaa
      961 ccgagttgaa ttcctgagat tgcacctgtt gcaaatgaag caaatttcgc agcagaggcg
     1021 gcttttgcag aatatatatt tgctgcatta ttagcggctc tgacggactg actaatttta
     1081 gataaatcat gtttcgtaac attgacttct cgagaaaatt ggctttgcat ttgccttaac
     1141 aaagatattt ctgctactcg tgctttatta tttatattat atttactctg catttttctt
     1201 acaacttgtc tcaactgact ttgtgttttt tgtaattcac gttgtaccga ttttcctaaa
     1261 ctttcatgtt tggctgcata attggtagaa tcagttgaca tttgtgtata aaaattttta
     1321 cgaaaatttt cgtaactcag attaattaca tccgtacttg cagttataaa atcttgtata
     1381 aattcaatag aaactgaatt atcatcagat ttggctttgt tattttccat attgagcctc
     1441 tcttaatttg aaaaaattaa cataaacatt taaaaataaa acaaaattat ataataaaaa
     1501 aaataatgag aatataaagg ataaaaagag agttaagtta tttaatcgga cttgttctct
     1561 ataaatcata aggcaccaaa taagccataa taaatttagt aaataaaaac tgcttatacc
     1621 caactttctt ttcttggaac ctattattat ttcaactctg gatgaaaaaa tagaatttaa
     1681 aaattttttt ctttgatata tcgttaatat ctccctagtt tttcttaaaa aaaatataat
     1741 catatatata ctacacaaca ttggtaataa ataaattaaa agcgaaaggt aattaaattg
     1801 ataattgtaa attatataat aaacaacata cgaagaaaaa attattatag atgatttgct
     1861 atacgaataa aaaaatatcc tacctagccc gaaccttctt ataatttgta ctatgtgatc
     1921 attcatttca atatccattt aagctttcaa ttaattttct atatttttca atggcatgta
     1981 tcatttggtt gatgatcaag ccatgtatgc aaagattgga atatggtatc tgtcgtgcta
     2041 ataatgcccc cgttactgtt taatttgcgc acaggaaaac cgtcatatgc agcaatccag
     2101 cccgtggcgg tgcggatacc ttggctgcgg tggtcgaaca atgcacctgc aaggcctttg
     2161 gtggcaacgg tttctatact gtcgccatct agatctaggg caagggggtc ggttacgtgg
     2221 aatttgtcgc tgcggttaag atttaagaaa tcttcaaacc atctccaagc tttttcagct
     2281 cctaatgatc cccaccaagc tcctgcgcca cctccaatta tagttccaac tactagccaa
     2341 cctgcgccac cagtaagtaa ataagtaaac caaaagcccc aacacccaaa gccgcacccc
     2401 attggaagcc cattagccca cccaaaaaag aagctgatga gagaccaaca tcactccaat
     2461 ttccttcttc atccaaggct ttccaccaag cacttcccat actcaaagtt cccatggcaa
     2521 cagttgcccc ataagtaaac ttacccaaat tcgctgccat aacttttgat tttgaagcat
     2581 tctctaacgc tgttgccgca cgtcttgcat attgctgctt gttatagttg ctagcattca
     2641 atgctttttg caaatcactt tgtacttttc ttgcaacaaa tgcctccttt gaattaccac
     2701 gaaatttact agcagctctt ccaattcctt cttttaagct tttttgtgcc tcttctgctg
     2761 tgcgttggaa ttctcttgcc ttagcagaca gacgctcacc ctcccttaaa aatgctcgtt
     2821 gatctatctt gtgcgaaagg gtcaaatatt cttcgtacat tgagttaatc gtaccaatcg
     2881 ccctaaccac attttcatca gaaaatatgg aaataatagc atccctatac gcacctagcg
     2941 taatattgtt tctattatta gttatagcat tattcgaata cataatagca cctccaaact
     3001 tagaactgaa tacgtttata ttctctgtgt aataatatca aataattaat taatggataa
     3061 gtaagtccga aaaaataaca actaaaataa gcaatatact gattctctcc tttattaatt
     3121 agatatattg aaaaacaaat aaaaaataaa cagaaaacat tcatgtgaaa aagccacctc
     3181 atattcttat ttataagttt aaaataacta ggcattgtat atttaaaaat cggtacatcc
     3241 aacggaaaca aaccacataa aaatttttct ttattcttta ttaaagacat agttttattt
     3301 ttcaaaatta gtccagataa tatactaaca attactatta aaacaataag cacagcgtaa
     3361 ttttttataa aattcattaa aataataacg aaaatactaa atagattaca tactgtaaat
     3421 tgaaatataa aatattcagt atttaatcca ttttcaaccg cgagtttttt aaaatatcct
     3481 tgctcgaaaa aattaaaaaa atccattaat actttcctct caagaaaact aattttagtt
     3541 tagttaagat aaaatgcaat attacctttt ctccataaaa gtatggagct tatatacata
     3601 aattatgtgc gaggtagcct gaaaaatatc caatacaaga tagtaggaga tgtttccctt
     3661 atcactattg gccaataccc aattggcagg cactctattt ccaaaatttt ctcaagaata
     3721 gttaaatatt attaattagt ttaaaaacaa taacatacgt ttgtatttat ccgttttggt
     3781 ctgcctgaaa cagactctat gtccatacac ggacatgtag acagtttcct tgttattttg
     3841 gttgctcgtc attagtgatg atactttact cctctcttaa aaaatcgtca agaactgtta
     3901 tcaatcttta aagtcttttt taaacaaata tatatacgtt caggctgtct gaaacacaaa
     3961 aaaatgatgt tacccgaccg tgacagagtc ggggcagccg gtaccttaac cctgcacctt
     4021 aaccctgtgc cttaacggta tataggaggc acgataggtg cgaggcatcc gcatctatct
     4081 tgattcctaa gccgttttac cttcctgaaa cgagaacttt tccatacttt ctctttacaa
     4141 ttcccatata tatccttaca attcgttctg ctgttttatt tatatatttc tttctactta
     4201 atatattcct tttccagcca gccgttatcc acatccaatc taactatatt ttatttatgt
     4261 ctatcgtatc cgcaccgctc cccgcccttt ccgccctcat catcctcgcc cattaccacg
     4321 gcattgccgc caatcctgct gatatacaac atgaattttg tgcttccgca caaagcgatt
     4381 taaatgaaac acaatggctt ttggccgcca aatctttggg attgaaggca aaggtagtcc
     4441 gccagcctgt caaacgtttg gctatggcga ccttacctgc attggtatgg tgtgatgacg
     4501 gcaaccattt tattttggct aaaacagacg gtgggggtga gcatgcccaa tatctaatac
     4561 aggatttaac tacgaataag tctgcggtat tgtcttttgc cgaattttct aacagatatt
     4621 cgggcaaact gatattggtt gcttcccgcg cttcggtatt gggcagtttg gcaaagtttg
     4681 actttacctg gtttattccg gcggtaatca aataccgccg gttgtttttt gaagtattgg
     4741 tggtgtcggt ggtgttgcag ctgtttgcgc tgattacgcc tctgtttttc caagtggtga
     4801 tggacaaggt gctggtacat cggggattct ctactttgga tgtggtgtcg gtggctttgt
     4861 tggtggtgtc gctgtttgag attgtgttgg gcggtttgcg gacgtatctg tttgcacata
     4921 cgacttcacg tattgatgtg gaattgggcg cgcgtttgtt ccggcatctg ctttccctgc
     4981 ctttatccta tttcgagcac agacgagtgg gtgatacggt ggctcgggtg cgggaattgg
     5041 agcagattcg caatttcttg accggtcagg cgctgacttc ggtgttggat ttggcgtttt
     5101 cgtttatctt tctggcggtg atgtggtatt acagctccac tctgacttgg gtggtattgg
     5161 cttcgttgcc tgcctatgcg ttttggtcgg catttatcag tccgatactg cggacgcgtc
     5221 tgaacgataa gttcgcgcgc aatgcagaca accagtcgtt tttagtagaa agcatcactg
     5281 cggtgggtac ggtaaaggcg atggcggtgg agccgcagat gacgcagcgt tgggacaatc
     5341 agttggcggc ttatgtggct tcgggatttc gggtaacgaa gttggcggtg gtcggccagc
     5401 agggggtgca gctgattcag aagctggtga cggtggcgac gttgtggatt ggcgcacggc
     5461 tggtaattga gggcaagctg acggtggggc agctgattgc gtttaatatg ctctcgggac
     5521 aggtggcggc gcctgttatc cgtttggcgc agttgtggca ggatttccag caggtgggga
     5581 tttcggtggc gcgtttgggg gatattctga atgcgccgac cgagaatgcg tcttcgcatt
     5641 tggctttgcc cgatatccgg ggggagatta cgttcgaaca tgtcgatttc cgctataagg
     5701 cggacggcag gctgattttg caggatttga acctgcggat tcgggcgggg gaagtgctgg
     5761 ggattgtggg acgttcgggg tcgggcaaat ccacactcac caaattggtg cagcgtctgt
     5821 atgtaccgga gcagggacgg gtgttggtgg acggcaacga tttggctttg gccgctcctg
     5881 cctggctgcg gcggcaggtc ggcgtggtct tgcaggagaa tgtgctgctc aaccgcagca
     5941 tacgcgacaa tatcgcgctg acggatacgg gtatgccgct ggaacgcatt atcgaagcag
     6001 ccaaactggc gggcgcacac gagtttatta tggagctgcc ggaaggctac ggcaccgtgg
     6061 tgggcgaaca aggggccggc ttgtcgggcg gacagcggca gcgtattgcg attgcccgcg
     6121 cgttaatcac caatccgcgc attctgattt ttgatgaagc caccagcgcg ctggattatg
     6181 aaagtgaacg agcgattatg cagaacatgc aggccatttg cgccaaccgg acggtgctga
     6241 ttatcgccca ccgtctgtcc actgttaaaa cggcacaccg gatcattgcc atggataaag
     6301 gcaggattgt ggaagcggga acacagcagg aattgctggc gaagccgaac ggatattacc
     6361 gctatctgta tgatttacag aacgggtaga aggcggggga tgggcgtgta ggctgctttt
     6421 ttcgtaacgt tctgcgtttt cagaccgtcg tttactgggt ttactgactg aaatgaaacc
     6481 aaagtgcagg ctgccggaaa gactttttct gtctttttag gcagcctgca cttctttttc
     6541 actcatctag agacctttgc aaaattcccc aaaatcccct aaattcccac caagacattt
     6601 aggggatttt ccatgagcac cttctttcaa caaaccgcgc aagccatgat tgctaaacac
     6661 atcgaccgct tcccgctatt gaagttggac caggtgattg attggcagcc gatcgaacaa
     6721 tacctgaatc gtcaaagaac ccgttacctt agagaccacc gcggccgtcc tgcctatccc
     6781 ctgttgtcca tgttcaaagc cgtcctgctc ggacaatggc acagcctctc cgatcccgaa
     6841 ctcgaacaca gcctcatcac ccgtatcgac ttcaacctgt tttgccgctt tgacgaactg
     6901 agcatccc
//
LOCUS       DAWFLT010000099         6791 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWFLT010000099 DAWFLT010000000
VERSION     DAWFLT010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.099"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            861..1307
                     /gene="yciA"
                     /locus_tag="WGR74_001901"
     CDS             861..1307
                     /gene="yciA"
                     /locus_tag="WGR74_001901"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ0906257.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPILAGLLPTP"
     gene            complement(1358..2179)
                     /locus_tag="WGR74_001902"
     CDS             complement(1358..2179)
                     /locus_tag="WGR74_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0906258.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADES
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDEKAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            2298..2756
                     /locus_tag="WGR74_001903"
     CDS             2298..2756
                     /locus_tag="WGR74_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ0906259.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PEFTRMGKMVKDEEPYDVEKFKQAAAAFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2920..4035)
                     /gene="lst"
                     /locus_tag="WGR74_001904"
     CDS             complement(2920..4035)
                     /gene="lst"
                     /locus_tag="WGR74_001904"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ0906260.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNHGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFKQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            4136..4240
                     /locus_tag="WGR74_001905"
                     /pseudo
     CDS             4136..4240
                     /locus_tag="WGR74_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            4468..6693
                     /locus_tag="WGR74_001906"
     CDS             4468..6693
                     /locus_tag="WGR74_001906"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ0906261.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLLANLDKLDADTRTAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKDGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALST
                     L"
BASE COUNT         1807 a         2003 c         1519 g         1462 t
ORIGIN      
        1 ggcgggaatc cagacccccg acgcggcggg aatctatcgg aaatgactga aaccccgcgt
       61 tctagattcc cacttccgtg ggaatgacgg ttcagttgcg ctccgacaac accgtaatct
      121 caaaacccgt ccgacaacac cgcaatcttg aaattcgtca ttcccgcaca ggcgggaatc
      181 cagacctgtc cgcacagaaa cttatcggat aaaaacagtt gcccaaaccc cgcgttctag
      241 attcccactt tcgtgggaat gacgtggtgc aggttccata tggatggatt cgtcattccc
      301 gcgcaggcgg gaatccaaac ccccgacgcg gcgggaatct atcggaaatg actgaaaccc
      361 cgagattcta gattcccact ttcgtgggaa tgacggttca gttgcgttcc gacaacaccg
      421 taatcttgaa atccgtccga caacaccgta atcttaaaac ccgtcattcc cgcgcaggcg
      481 ggaatccaga cctccgacgc ggcgggaatc tatcggaaat gactgaaccc cccgagattc
      541 tagattccca cttccgtggg aatgacgtgg tgcaggtttc cgtatggatg gattcgtcat
      601 tcccgcgcag gcgggaatct agacctgtcc gcacagaaac ttatcggata aaaacagttg
      661 ctcaaacccc tcgttctaga ttcccacttc cgtgggaatg acggttcagt tgctacggcc
      721 ccgccctaca atacgccttg cgaatctgtc ccacgcccgc ccttgtatgt ctcaaatggg
      781 ttaaactacg cacttcccga cttcccgttt cgggcagggg tgcaggttcg atttatcttt
      841 caataaaaca aggaaacatt atgcagcacg aagaaggcaa ccgccaacgc cctcaaggcg
      901 aactgctcct gcgtaccgtc gctatgccgc gcgataccaa tcccaaccaa gacattttcg
      961 gcggctggat tatgtcgcag atggatttgg gcggcggcat attggcggcg gaaatcgcgc
     1021 ggggacgcat cgttaccgtc gccgttcagg aaatgaactt tatccgcccg gtcaaggtcg
     1081 gcaacgtcgt ctgctgctac gggcattgcg tccgcgtggg caatacttcc ctccagctta
     1141 aagtcgaagt ctgggtgaaa actttgatga acgattgcgt taccgaagac cgccacctcg
     1201 taaccgaagc cgtgttcact tatgtcgcca tcgatgcgga aggcaatccg cgcccgattc
     1261 cgaaagaagg caaccccata ttggcgggct tattgcctac tccgtaaaaa tacccgtaaa
     1321 aatgccgtct gaaactgtct tcagacggca ttccgcatca tttgaaccaa gatctgatgc
     1381 gggaaaacag cgacatactc tgtttttcaa agtcttcccg cacttccggc acggcttttt
     1441 ttatcacttt cattcctgca aaagtctgcg ccacgggcat aatttcaatc gtgttcacat
     1501 tcatatgcgc cggccggcgg tacagccaca atgcggtttc cgcaatatct tcggggcgga
     1561 taaattccac accctcatac acgcctgctg ccttctcgtc atcgcctttg aagcgcacgt
     1621 tggaaaactc cgtattgccg cacaaacccg gctcgatatt ggtaacgcgg atgttcttat
     1681 ccgccaactc cgcgcgcaaa ttcaggctga actggcgcac aaacgccttg gtcgccccgt
     1741 aaacgttgct gccggcataa gcataattgc ctgcaatcga acccaaattc atcacataac
     1801 cgccgccgcg ttccaccatt tgcggcaaaa ttttgcgcgt caggaacgtc aaacccaaaa
     1861 cattggtttg aatcatcgtt tcccaatctt caaaatccgc cttgtccgcc gtgtccagac
     1921 ccaaagccag cccggcattg ttgatgaggc agtcgatgtc ggaaaattca tcggggatgc
     1981 cgtttaaggc gttttccacc gactcgcgac gcgacacgtc catttccaaa gggtaaaaca
     2041 aagcacccga ttcatccgcc aaagcctgaa gcctgtccgc gcggcgcgcc gcaccgataa
     2101 cgcggtatcc cgccccgaca aacgcacggc acatcgcttc gccgaatcct gccgaagcac
     2161 cggtaattaa aacagccatt gtttttcctt tctttctcgt ttcccaaacc tgtttcaggt
     2221 atcataacac ccttcagacg gcatcgcgcc gactgaaaat gacaacaacc ataccaccac
     2281 gacaggacac attcaatatg aaaacccaaa tcagccttgc tgctgccgcc atcacactgc
     2341 tcctttccgc ctgcggaggc agcggaatac cttcccaacc caaaggcgaa atttccgaaa
     2401 accgcaccgc cgcgttcaaa tccatgatgc ccgaattcac acgcatgggc aaaatggtca
     2461 aagacgaaga accttacgat gtcgaaaaat tcaaacaggc tgccgcggcg tttgccgaaa
     2521 gcagcaagaa accgttcaca ctttttgagt ccgatccgca aggcaacggc cgcgccctgc
     2581 ccgccgtttg gtcggatggt gcaaaatttg aagccgaaaa aacaaaattc gccgccgccg
     2641 tcgaaaaact caacgccgca gcacaaaccg gcaaactgga tgaaatcaaa gccgcctacg
     2701 gcgaaaccgg cgcaagctgc aaatcctgcc acgacagttt ccgcgcgccc gaataacatc
     2761 caaaacaaaa caatgccgtc tgaagccttc agacggcatt ttctttgcca tttcagcaca
     2821 cgcaaagcaa tcagaaatta tcttcaattc aaaaaatacg ccatatcctg atttttgcct
     2881 tacttactcc gccattttct attttgattt gctatgcgat taatctttat cgtcaaatgt
     2941 caaaatcggg atgccggatt gggtaaacag ggcatacacc ggcttgagcc aataatcttc
     3001 cggaagggaa gccggtttca atgcgtaaac gtgcacattg ggaaaatcct tcatcgtcaa
     3061 cgccgcgccg ctgaaaaagg tataaatttc atacctcgta tgcgggtttt tcttaatctc
     3121 gcgcaaaata tagtcttcga tgacataggg cgaatttaat gtggttacgc cggaaagccc
     3181 gtaggtttgg cgggggtgcg gtgcgacata ttgtatgttg aagttttttg ccgccttttc
     3241 cgaaatttcc ttcatctcct tgtcgggcga acccaaaagt atccgcaccg tgccgcccgt
     3301 ttcgtccccc gccttcagtt cggacgcatc gaacagcggc aggtaagtca tcttgcggcg
     3361 gccgtcgtcc ataatgtttt tcaaaccctt gaatatcgtg taatgctcgt cggaagcatt
     3421 acgggttttg gcgatgctcc aatctccgat catcatccgg gcaaaattcc gcttgatcgt
     3481 cccgtttaca gaaaactcat cgcccaaata gctgctgctt tgaattaaat tgcctgtccc
     3541 gtcgtcaaag gttttgattt ccgcatccgg gtaagtgctc aaaaaggcgg caatgctgac
     3601 tttttccaaa cttgccaaat aaatccgctt gactttcggc agcagcatcg actttacctt
     3661 cagctccgcc atcgtcggaa tgaaattaaa cgatttgttc aaaccgtagg gcaggtggaa
     3721 aaaatacgcc cgctccgcct tatcctttat ctgcttgaaa taataatcgt atttttcatt
     3781 cctgttttca gacatcagca ccacataaaa ccgctccccc ggatgctgcg ccataatcct
     3841 ttccgccacc ttcatctgca atatggtata gcagaaaatc agattgaccg gttccccctc
     3901 ttcattgaag agtttgtcct tcagcaggga aaccgcattc ctttccccat gatttacccg
     3961 gtcaaatgta taaaatatcc cgaaacaaaa aacaatcaaa cacaacacgg tcaaacaagc
     4021 ctttttcaag cccatatccc taaaactcca ttccgacaaa ttgaacatac gccccgagtg
     4081 tcccgggacg gaaaaatgcg gaattttagc agatattccg acagttaccg aatattatag
     4141 tggtttaaat ttaaatcagg acaaggcgac gaagccgcag acagtacaag cagtacgaca
     4201 aggcaaggca acgctgtact ggtttaaatt taaaccacta taaaatcaat ttcaccgaaa
     4261 atatgccgcc gcacatcagg cacagcacct ccgaccgcca ccaaacgcgg gaaaaccatt
     4321 tcccccttcc tttcacgctg ccgcgttcaa acacgccaag atcgaacaaa gcgcgaacaa
     4381 agccgccaaa atatgccata atagccgcgc acattccccg cgcaatgtgt tccaaaagaa
     4441 aaaaccaacc tacaagagaa taatcctatg actcaaaaat ccaccattgt ttatacccat
     4501 accgacgaag cccccgcgct ggcgacccaa tcgctgctgc cgattgtgca ggcttttgcc
     4561 cgccacgccg atattgatgt caaaactgcc gacatttccc tgtccggccg tattttggcg
     4621 gcattccccg aatatctgac cgaagcgcaa cgcgtacccg acgcgcttgc cgaattgggc
     4681 gaactggtaa aacaacccga tgcaaacgta atcaaactgc cgaacatcag cgcatccgta
     4741 ccgcaactga ccgccgcgat taaagaattg cagtctaaag gctttgccgt tcccgattat
     4801 cctgccgacc cgcaaaccga tgaagaaaaa gccgtgcgcg aacgctacga ccgcatcaaa
     4861 ggcagcgcgg taaaccctgt cctgcgtgaa ggcaactccg accgtcgcgc ccccaaagcg
     4921 gtgaaaaact ttgccaaaaa aaatccgcac agcatgggcg catggacaaa agactccaaa
     4981 acccacgttg ccaccatgca aagcggcgac tttttccata acgaacaatc cgttaccgta
     5041 cctgaagcga cttccgtatc catcgtgttc accgacaaac aaggcaataa aaaagagctg
     5101 cgcgcgcctg ttgccctgaa agccggcgaa atcatcgacg caaccgtgat gagcaaaaaa
     5161 gccctgctcg ccttccttgc cgaacaagtg aaagacgcga aggcaaaagg cgtattgttc
     5221 tcactgcata tgaaagccac gatgatgaaa gtgtccgacc cgattatctt cggacacgcc
     5281 gtcaaagtgt tcttcgcgcc tgtatttgaa aaattcggcg gcaaactggc tgccgcaggt
     5341 gtcaacgtca acaacggttt cggcaacctg cttgccaatc tggacaaact ggatgcggac
     5401 acccgcaccg ccgtcgaagc cgaaatcgcc gccgtttacg ccgccaaccc cgatttggcg
     5461 atggtcgatt ccgataaagg cattaccaac ctgcacgttc ccagcgatgt catcgttgat
     5521 gcttctatgc ctgcgatgat tcgtaactcc ggccgtatgt gggataaaga cggcaaagcg
     5581 caagacacca aagccgtgat tcccgacagc agctacgccg gcgtttacca agcaaccatc
     5641 gacttctgcc gcgaacacgg cgcatttgac ccgacaacca tgggtactgt gcccaacgtc
     5701 ggactgatgg cgcaagcggc ggaagaatac ggctcgcaca acaaaacctt tgaaatcgaa
     5761 gccgacggcc aagtccaagt cattgatgcg gcaggaaaag tcctaatgca gcacgacgtt
     5821 gaagccggcg acatctggcg tatgtgccaa accaaagacg ctccggttaa agactgggta
     5881 caacttgccg tcaaccgcgc ccgtctgagc aacacgcctg ccgtgttctg gctcgacgaa
     5941 aaccgtccgc acgacaagag cctgctcgcc aaggttaaag cctaccttgc cgaactggat
     6001 accaatggcc tcgacatccg cgtcctcgct cctgaagaag ccgccaagtt cagcttgggt
     6061 cgtctgaaaa acggcgaaga caccatctcc gtaaccggta atgtcttgcg cgactacctg
     6121 accgacttgt tcccaatttt ggaactcggc acaagcgcga aaatgctgtc tatcgttcca
     6181 ttgatgaacg gcggcggtat gtttgaaacc ggcgcgggcg gttctgcacc gaaacatgtt
     6241 caacaattcc tcgaagaaaa ccatttgcgc tgggactcgc tgggcgaatt cctcgcactc
     6301 gccgtatcgt ttgaacattt ggcgcaaaaa accggcaatg ccaaagccca agtcctcgcc
     6361 gacactttgg atgcagccac cgaaaaactg ctgttgaacg acaaatcgcc taaacgtaaa
     6421 gccggcgaac tcgacaaccg cggcagccat ttctacctca ccctctactg ggcgcaagaa
     6481 ttggcggcgc aagacaaaga tgccgaactg aaagccgcat ttacgccatt ggcagccgct
     6541 ttgaccgccg acgaagcgaa aatcgttgcc gagctttccg ccgtacaagg caaagcggcc
     6601 gacatcggcg gctactacgc cgccaatcct gaaaaagccg cacaagtgat gcgtccgagc
     6661 gtaaccttta atcaggcact aagcacctta taagcacaaa agtaaaaatg ccgtctgaac
     6721 atttatcgtt cagacggcat tagttatccc tatctgcctg attatgatta aacgccgcac
     6781 ccattggata t
//
LOCUS       DAWFLT010000100         6749 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWFLT010000100 DAWFLT010000000
VERSION     DAWFLT010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.100"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(117..749)
                     /locus_tag="WGR74_001907"
     CDS             complement(117..749)
                     /locus_tag="WGR74_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906262.1"
                     /translation="MEKIEKFKSELLNAIFQYTQCISIFVYKKNIYYLIDCKENFILN
                     MKLDLDLDFKNGNITLEQYQDEMNSYYYRNGIWQLTKDNFESYLQSDSVIELKKDELK
                     KLMFQGFTSAEAVRLYSVVENKLSYNDPISDSGQQSDFLKINQISSRLPLFYVNFDTE
                     VYLHMDWDRCHEDYVYDGWFSKAMDFGYLIPDEFCYWKIEGRDYWKFRQL"
     gene            complement(904..1398)
                     /locus_tag="WGR74_001908"
     CDS             complement(904..1398)
                     /locus_tag="WGR74_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
                     /protein_id="HEZ0906263.1"
                     /translation="MIFKQNQNYWAVFYTNEEALIVQTCSGLGLTAIDHLYPPHILPL
                     DTDNETLGTTVLQALANSRTFVYDSPEDQDFFDTEKIRQRYEDWVAKLCGNLGYKTKR
                     ALFKNMMSVDIWLHNGCLKISPSRHVKLDAWDAIDADDVILSLDNSPEEIGTGLRLAL
                     SRCR"
     gene            1722..3155
                     /locus_tag="WGR74_001909"
     CDS             1722..3155
                     /locus_tag="WGR74_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ0906264.1"
                     /translation="MNEYYQLIKHPDISLSPISDGIGVGNPATGEILAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTESRGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIIKPASLTPLSAYALASLAYEAGIPQDLLPVVSGRASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDEFCRKLSEKVAELKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALAKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            3264..4052
                     /locus_tag="WGR74_001910"
     CDS             3264..4052
                     /locus_tag="WGR74_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ0906265.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVIEAFEKDRINVVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEKIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            4420..5976
                     /locus_tag="WGR74_001911"
     CDS             4420..5976
                     /locus_tag="WGR74_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0906266.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            5993..6739
                     /gene="surE"
                     /locus_tag="WGR74_001912"
     CDS             5993..6739
                     /gene="surE"
                     /locus_tag="WGR74_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ0906267.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEKALWTLLAHFFKNPPQSPILWNIN
                     IPAVEPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGRVSDKEEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHAD"
BASE COUNT         1672 a         1631 c         1753 g         1693 t
ORIGIN      
        1 attttgcaaa ggtctcggcc tgagtgttgt tttacaggaa agtaaaggcc gtctgaaagg
       61 gtttaccagc tttcagacgg ccttttgaat tcatgcaagc tatggcttgc ttatatttat
      121 aattgtctaa atttccaata atcccgtcct tcaattttcc aataacaaaa ttcgtccggt
      181 attaaatacc caaaatccat cgcctttgaa aaccagccat cataaacata atcttcatga
      241 catctgtccc aatccatatg taagtacact tcagtgtcaa aattgacata gaataaaggc
      301 agcctcgagg aaatctgatt gatttttaag aaatcacttt gctgtccaga gtctgagata
      361 ggatcattgt acgacagctt gttctcgaca acggaatata accttaccgc ttcagcagaa
      421 gtaaatccct gaaacatcag tttcttcaat tcatcctttt ttaattcaat gactgaatct
      481 gattgaagat agctttcaaa attgtcctta gttaactgcc agatgccgtt tcggtaataa
      541 taactattca tttcatcctg atattgttct aacgtaatat taccgttttt aaaatctaaa
      601 tctaagtcta atttcatgtt taatatgaaa ttttccttac aatcaatcaa ataatatata
      661 tttttcttat agacaaaaat agatatgcat tgagtatatt gaaagatggc atttaataat
      721 tcagatttaa atttctcaat tttttccata tttgccaaat tccttaatag ctttatcttc
      781 agcaagtcgt agcccgcatt aaatctgcag gtaaggtgtg gtacgcacgc cagtgagctt
      841 gagtctaatg aaaaaggtcg tctgaaacat ttttccggct ttcagacgac cttttgtcaa
      901 atattatcgg cagcggctca atgctaacct taaacctgtt ccgatttctt caggactgtt
      961 atccaatgat aaaattacat cgtctgcatc aatggcatcc cacgcgtcca gcttgacatg
     1021 gcggctcggg ctgattttca ggcagccgtt gtgcagccaa atatccacgc tcatcatgtt
     1081 tttaaatagg gcgcgtttgg ttttatagcc caagttcccg catagcttgg caacccaatc
     1141 ctcatagcgt tgccgaattt tttcggtatc aaaaaaatct tggtcttctg gactgtcata
     1201 aacgaaagtc ctgctgtttg ccaatgcttg caagaccgtt gtgcctaaag tttcattgtc
     1261 ggtatccaat ggcaggatat gagggggata taggtggtct attgccgtta atcccaaacc
     1321 tgaacatgtt tgaacaatca gagcttcttc attcgtataa aaaactgccc aataattttg
     1381 attctgttta aaaatcatta ttttatctcc gtaattttga ctgtaatgtt ttgacttttg
     1441 ccatactcta ccacacgttg caactgcaat ctttgttcct tatatattat caaagggctg
     1501 cattactttt ctttctaaat taccattgta taaaaagtat ttttcttaaa cgacagggat
     1561 tgcgcccgcg ccatattcag acaccgcccg gatgttgcac tgcccgatcg gatgcttcag
     1621 acggcatcgg aagggtttgc agttttggaa tatgaagatg ataatgtccg cgagattgac
     1681 ggcatttgaa agcggttatt ccgcataccg gaggatacga aatgaacgaa tattaccaat
     1741 taatcaagca tcccgatatt tccctttccc cgatttcaga cggcatcggg gtcggcaatc
     1801 cggcaacggg cgaaattttg gcgtatgtcc gcaatacggg ttcggacaaa ctgaaaaacc
     1861 tgattcaaaa ggcggcggca gcgcaaaaat tatgggcggc gaaaactgct ttggaacgcg
     1921 ccgacatatt gtggcgttgg tattttttag ttaaagaaaa caaagaagca ctggcgcgcc
     1981 tgatgacgat ggagcagggc aaaagcctga ccgagtcgcg cggcgaaatt gattatgcgg
     2041 cttcgtttgt gcgctggttt gccgaagagg cgcggcggat tgacggcgat gtgttaacga
     2101 gtgtaaaagc gtcgcaaaaa ctggttgtgt tgaaacagcc tgtcggcgtt accgccgcga
     2161 ttacgccgtg gaacttccca tctgcaatga ttgcgcgcaa ggctgcgcct gctttggcgg
     2221 tgggttgcgc gatgatcatc aaacccgcat cgctcacgcc tttgagtgcg tatgccttgg
     2281 cttcgttggc ttacgaagcg ggcataccgc aggatttgtt gcccgtcgtc agcggccgcg
     2341 catcggaaat cggccgtgaa tttgccacga acccgattat ccgcaaaatc agcttcaccg
     2401 gttcgaccga agtcggcgcg aaaatttttg ccgacagcgc ggcggacatt aaaaaactca
     2461 gtttggaact gggcggcaac gcgccgttta tcgtgtttga cgatgccgat ttggacaaag
     2521 ccgtcgaagg tgcgctcgcc agcaagttcc gcaacagcgg tcagacctgc gtctgcacca
     2581 accgcgttta cgcccaatcc ggcatttacg acgaattttg tcgcaaattg agtgaaaaag
     2641 tagccgagct caaattgggc aacggcttgg aggatggcgt caaccaaggg ccgctgattg
     2701 aggaaaaagc ggtagaaaaa gtcgagcagc acattgctga cgcgctcgct aaaggcgcgg
     2761 tctgtctgac cggcggcaaa cgcagcgcat tgggcggaac gttttttgag ccgaccgtct
     2821 taagcggcgt aacggcgcaa atggcggtgg ctcgcgaaga aaccttcggg cctttgtgtc
     2881 cggtattccg ttttgaaacc gaagccgaag tcattgaggc tgcgaacaat acggaatacg
     2941 gtttggctgc ttaccttttt accgccgaca ccgcacgcca gtggcgcgtc ggcgaagcat
     3001 tggaatacgg tatggtcggc atcaatacgg gcttaatcag taatgaagcg gcaccgttcg
     3061 gcggcgtgaa acgcagcggt ttgggacgtg aaggcagcaa atacggtgcg gacgaatatc
     3121 tggaattgaa atatctgtgt atggatgtcg ggtgacggat gccgtctgaa cggcagggtt
     3181 tcagacggcg ccgcatttta agcagtctct atctgttgta caatgcgccc tgtttttacg
     3241 gttatttttg atttgaacaa gatatgatgg aaaacggaaa aacagtcccg agatggcgtt
     3301 ttgccttgaa aagtgcgggc tggcacctct taatcagcct gtcggttgca gggctggcgg
     3361 cattgctggt ttttaaggtt tggtatcctt atccttatgc cgagctgacg ggagggctgt
     3421 cgctttatca gctggtggtg gctgtcgata ttgtatgtgg tccgctgctg actttaattt
     3481 tggcaagccc gaagaaaaag acaaaggcac gcatggtcga tttttccatg gtcggcatca
     3541 tccagctggc ggctttggtg tacggtctgc acagcgtttc tttggcgcgc cctgtgatag
     3601 aggcgtttga aaaagacagg attaatgttg tgaccgccgc cgaagtggcg gttgaagatt
     3661 tgcacaaagc gccggaaggg ctgcaaagcc tgtcgtggtt cggcatccgc cgcattgcat
     3721 tgaaagaacc tgaggatgcg gatgagaaga acaagacgct ggatttgtcc ctgaaaggta
     3781 tcgagccgag tatgcgtccc gaccggtggc tgccgtattc cgacaaggaa gcagaaaaaa
     3841 tccgcaaaca tctgaaaccg ctgaaagtct tggcggatgc gagaaaaacg acggttgcgg
     3901 acattctgaa acaggcaggt ctcgccgaag gggaggagct gtattacctg ccgtttacca
     3961 gcagcaggca gaaagagtgg atagtcatta ccgataaaga gggcaacacc aaaggctacg
     4021 cgccgataga cggcttcatc atcacccctt aagcgttggg actccgtccg cactcgaaca
     4081 tccgttcttc gcggcggtag aatcagactg tatttgagag gggcgcaacc cctcaatcag
     4141 ggcaacacat aggggaaggc tttatgtgcc gtccgtgtgt tgaaacattt caaaataagc
     4201 tcatgccgtc cgaacatcct ttcagacggt atggcgtttt gccattgccc cgataagctg
     4261 ttaaactatt taaattattt cgaccgaagg tacacgcaca tgcaagaccc cgagcagagc
     4321 agcaagcccg cccgccgttt tttgtgcgta ccgtctgttc aggcagcttg agcgcttcag
     4381 gctgcctttt cgcgtttgaa cttgaggaat acgaaaatca tggatttcag ttggttggca
     4441 gaaccgcata cctggatagg ttttgccacg cttttggtgt tggaagtcgt attggggata
     4501 gacaatcttg tctttgtggc gattttggca aacaaggtcc agcccgcacg gcgcgaccgc
     4561 gcacggatta tcgggctggg gctggcagtc gtcatccgca tcattatgct tgcttttatg
     4621 gcgcacatca tcacgctgac cgagccgctg ttccaaatcg gcggcctcgc cgtttccggc
     4681 aaggacatga ttatgctcgc gggcggtatt ttcctgcttt acaaagccac caccgaactg
     4741 catgaacgcc tcgaagggca caaccgtttt accgttgccg acaaccaaaa aaaacacgcg
     4801 ccgttttggg gcgtggtcgc gcaaatcctg atactggatg ccgtgttttc catcgattcg
     4861 gtcattactg cggtggcgat ggtcgatcat atcgtcgtgg cgatgggtgc ggtcgtcgtc
     4921 gcgatggctg taatgatttc tgccagcaaa ctcttgaccg aatttgtcga cagacaccct
     4981 accgtcgtga tgctctgcct tggttttttg ttgatgatcg gtttcagcct gattgccgaa
     5041 gccttccatt tccacattcc caaaggctac ctctacgccg ccatcggctt ctcgatttta
     5101 atcgaattgt ttaaccagat ttcgcagcgc aacagccgca aaaacgacta catcggcagc
     5161 tcgtggcgca agcgcaccgc cgaaaacgtc ttgggtatga tgggtatacg cgaaagcgtg
     5221 cttgccgacg cgggcggcga atccggggac gacgcgcatt ttgaagaaaa cgaaaaatcg
     5281 atgatacgca gcgtgctgac gcttgccgaa cgcccgatta tgggggtgat gatcccacgc
     5341 cgcgacatcg aacggctgga catttcccaa agccgcgaag aacagtgtgc ccaactgcaa
     5401 aacacgcctt acagccgcct gctcgttgtc ggtaaggcgg gcgtggacga acctttgggc
     5461 tacatcaaca aaaaagacct gctgtcccaa ctgctggaaa caggcggtct cgacattcag
     5521 acggcattgc gccagccact cgtcctgccc gacagcacca ccgcgctggg cgcaatcgaa
     5581 ctcttccgcc aaagcagcgc ggattatgct ttggtggtgg acgagttcgg cgcggtattg
     5641 ggcatggtaa ccatgaaaga cctgctcgaa accatcgcag gcgagttccc cgaagaattt
     5701 gagcgcgaag aagaaccagc cgttcagggg aatcccgatg aaagcctgac ggtggaaggc
     5761 gcgttggaat atgtggaact cgcaccgcaa ctcaacctgc cgcagcagga ggaagatgcc
     5821 gatttccata cggttgccgg gctgattatg gaagaattgc aaaccatccc cgatgtcggc
     5881 gattttgccg atttccacgg ctggcggttt gaagtggtcg aaaaagaagg gcagcgcatc
     5941 gagcgggtca aaatcaccaa attgcccgaa gaataagcat tcaggataga aaatgaacgt
     6001 tttgatttcc aacgacgacg gctacctctc cgaaggtatt gccgttttgg cgcgcgttac
     6061 ggcggaattt gccaacgtca gggtggttgc gcccgaacgc gacaggagcg gggtcagcaa
     6121 ttcgctgacg ctggaacgcc ctttgcagtt gaaacaggcg caaaacgggt tctactatgt
     6181 caacggcacg ccgaccgact gtatccacat cgggcagtct gtattttcgg attttcaggc
     6241 cgattttgtc ttttcgggca tcaaccgggg cgcgaatatg ggggacgaca cgctttattc
     6301 ggggacggtt gcggcggcaa ccgaagccta ccttatgggc atacccgctg tggcgttttc
     6361 cttaaacgac gcttccggac gctattgggc aaccgcagaa aaggcactgt ggacattgtt
     6421 ggcgcatttt ttcaaaaacc ccccgcagtc ccctattttg tggaacatca atatccccgc
     6481 cgtcgagccg gaagatgtgc ggggcattaa aatcgcccgt ttgggcagga ggcatcacgg
     6541 tcagaacgtc atccccgcgc gcaatccgcg cggcgaacag atttattgga taggcccggt
     6601 aggcagggtt tccgacaaag aagagggaac ggatttcggt gaatgcggcg caggtttcat
     6661 taccgtaacg ccgctgcaaa tcgacctgac cgcctatccg gacatggcgg aaacagcggc
     6721 gttctggcat gcggactgac cgtttcatc
//
LOCUS       DAWFLT010000101         6731 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWFLT010000101 DAWFLT010000000
VERSION     DAWFLT010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.101"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..687
                     /gene="mafB"
                     /locus_tag="WGR74_001913"
     CDS             <1..687
                     /gene="mafB"
                     /locus_tag="WGR74_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0906268.1"
                     /translation="PAEGKFAAIGGLGSVAGFEKNTREAVDRWIQENPNAAETVEALV
                     NVLPFAKVKNLTKAAKLGKAAVSGDFSAAYNTRTTRKVTTETEGLNRIRQNQKNSNIH
                     EKNYGRDNPNHINVLSGNSIQHILYGDEAGGGHLFPGKPGKTTFPQHWSASKITHEIS
                     DIVTSPKTQWYAQTGTGGKYIAKGRPARWVSYETRDGIRIRTVYEPATGKVVTAFPDR
                     TSNPKYNPVK"
     gene            702..932
                     /locus_tag="WGR74_001914"
     CDS             702..932
                     /locus_tag="WGR74_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ0906269.1"
                     /translation="MKTLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            1081..1755
                     /locus_tag="WGR74_001915"
     CDS             1081..1755
                     /locus_tag="WGR74_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ0906270.1"
                     /translation="MVKTADGYKAIAHIQAGDRVFAKDETSGKTGYKPVTAQYGNPYR
                     ETVYIEISDGIGNNQTLISNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGNQAETEGVWVHNECPTKPKPTNHAQQRKEEAKNDSH
                     RSVGDSNRVVREGKQYLDADTGNHVYVKGEKVVILTPDGRQVTQFKNSKANTSKRVKN
                     GKWTPK"
     gene            1733..2014
                     /locus_tag="WGR74_001916"
     CDS             1733..2014
                     /locus_tag="WGR74_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906271.1"
                     /translation="MENGHRNNRSPLEKRIFYLEHSGQYLMICALSDYSKNKHTVVMA
                     NFLYPNEKMDWRNLDDLFNELVLEELQSSFMDWYPTIEEAISHHLEDFS"
     gene            2070..2243
                     /locus_tag="WGR74_001917"
                     /pseudo
     CDS             2070..2243
                     /locus_tag="WGR74_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pretoxin"
     gene            2454..2681
                     /locus_tag="WGR74_001918"
     CDS             2454..2681
                     /locus_tag="WGR74_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906272.1"
                     /translation="MGVNLIGLNFEDFKQLFHHPKSYGVDEYYLSNESYPTYVYEFDE
                     IGVQAWEAKGKVVTIIAGGKDNYSTEPYYDE"
     gene            2688..2744
                     /locus_tag="WGR74_001919"
                     /pseudo
     CDS             2688..2744
                     /locus_tag="WGR74_001919"
                     /inference="COORDINATES: protein motif:HMM:NF026864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2750..3127
                     /locus_tag="WGR74_001920"
     CDS             2750..3127
                     /locus_tag="WGR74_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ0906273.1"
                     /translation="MCEFKDFRRNIPCFEEYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRDIVIGIGTIIDFLMVPNWELFEIKASPWLPDSVGIHERYERFT
                     TMLRYIFTEKDIVNVRFDYYNKK"
     gene            3432..3833
                     /locus_tag="WGR74_001921"
     CDS             3432..3833
                     /locus_tag="WGR74_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906274.1"
                     /translation="MKIKPLQFSNNSRRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFGF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            3999..4448
                     /locus_tag="WGR74_001922"
     CDS             3999..4448
                     /locus_tag="WGR74_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906275.1"
                     /translation="MENRFEKIVKDIFSFTQFVSEAENEIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKHLFAEEVPKNNIVCNISHFLHFADRA
                     EADIPNQSRSYAEYWWEMEMFKMSVLEDWERSGRPLNWEKWQRQYRS"
     gene            4622..5755
                     /locus_tag="WGR74_001923"
     CDS             4622..5755
                     /locus_tag="WGR74_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906276.1"
                     /translation="MFKKFKLVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNAFVAKLARLFRNADRAVVIVKESIRTEENLAETVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGKEVWLDYHIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     IREEKQGDKLPDFPLNLENLEKEVSRYAEAAARRSGGRRDLSH"
     gene            complement(5842..6118)
                     /locus_tag="WGR74_001924"
                     /pseudo
     CDS             complement(5842..6118)
                     /locus_tag="WGR74_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6135..>6731)
                     /locus_tag="WGR74_001925"
     CDS             complement(6135..>6731)
                     /locus_tag="WGR74_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0906277.1"
                     /translation="YGYLARYTQDNATKWLSDTPGQDAYSINLIEISVYYKKTDQGWV
                     LEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERLLTYGVKKMPSAYPEY
                     EAYEDKRHIPENPYFHKLYYIKKGENPAIITHRNNRINQTEEDNYSTSVGSCINGFTV
                     RYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNPSKK"
BASE COUNT         2215 a         1240 c         1440 g         1836 t
ORIGIN      
        1 cccgccgagg gcaaattcgc cgccatcggc ggcttgggca gcgtggcggg ctttgaaaag
       61 aatacgcgcg aagccgttga ccggtggata caggaaaacc ccaatgccgc cgaaaccgtc
      121 gaagccctgg tcaacgtcct gccgtttgcc aaagtcaaaa acctgacaaa ggcggcaaaa
      181 ctggggaagg ctgcggttag cggggatttt tctgctgcat acaatacaag aacaactaga
      241 aaagttacta cagaaacaga ggggttaaat agaatcagac agaaccagaa aaatagtaat
      301 atacatgaga aaaattatgg aagagataat cctaatcata ttaatgtttt atctggaaat
      361 tctatacaac atatactgta tggagatgaa gcaggaggtg ggcatctttt tcctggcaaa
      421 cctggtaaga caacattccc ccaacattgg tcagccagta aaataactca tgaaattagt
      481 gatatcgtta catccccaaa aacgcaatgg tatgcacaga ctggaacagg cggcaaatat
      541 attgctaaag gaagaccagc taggtgggta tcatatgaaa cgagagatgg aattcgtatc
      601 agaacagttt atgaacctgc aacaggaaaa gtggtaactg cattccccga tagaacctct
      661 aatcccaaat ataaccctgt aaaataatga gtatttgaaa gatgaaaaca ttagacgaac
      721 gaataaaaaa tttaggaaaa tctcttgaag atagaattga cgctaatcta attgatgccg
      781 cactagagta cattactttt tcagaacgtt tgctagcttt tgaaacgcta tgtgattata
      841 tagaagattt caatatccag cttacggaaa aagaatccca agaaatctct tttattaata
      901 aggaatttgg aattgaaagt acatcagatt aataaatatt aggtcggttt aaaaacccaa
      961 caatcaaaaa gtcgtctgaa actgtgttta atttacagac gacctttcct tcatttgaag
     1021 caggatattg agaactgagt tcttcaaaaa tcctacacct gctccttcca cggcagcacc
     1081 ttggtcaaaa cggcagacgg ctacaaagcc attgcccata ttcaagccgg cgaccgcgtc
     1141 ttcgccaaag atgaaaccag tggaaaaact ggatacaaac ctgttaccgc ccaatacggc
     1201 aatccgtatc gggaaaccgt ttacattgaa atttcagacg gcatcggcaa caaccaaacc
     1261 ctgatttcca ataaaatcca cccgttttac agtcaaggaa aatggataca ggcaggtcgt
     1321 ctgaaaaaag gcgacaccct gctttccgaa agcggtgcaa aacagacggt tcaaaacatt
     1381 accctcaaac agcagccgct caaagcctac aacctgaccg ttgccgactg gcatacctat
     1441 ttcgtcaagg gtaatcaggc ggaaacggaa ggggtttggg ttcataatga gtgtccgacc
     1501 aaaccaaaac caactaatca tgcccaacaa agaaaagaag aagctaaaaa cgattctcat
     1561 cgaagtgtgg gagattccaa tcgtgtcgtt cgcgaaggaa agcaatattt agatgccgac
     1621 acaggaaacc atgtttatgt aaaaggagaa aaagtggtta ttctaactcc tgatggaaga
     1681 caggtaactc aatttaagaa ctcgaaagcc aatacgtcaa aaagggtaaa aaatggaaaa
     1741 tggacaccga aataatcgat ctcccttaga aaaaagaatt ttttatctgg agcattcggg
     1801 acagtatctg atgatttgcg ccctatcaga ttactccaaa aataaacata ctgtcgttat
     1861 ggcaaatttt ctctatccaa atgaaaaaat ggattggaga aatttagatg atttgttcaa
     1921 tgagttggtt ttagaggaat tacagtcttc atttatggat tggtatccaa ctattgaaga
     1981 ggcaattagc catcatttag aagatttttc gtaacaagca taaatcaaaa agtcgtctga
     2041 aactgctttt aattttcaga cgaccttgag atggcggtcg aagtcaaata tcagcggaat
     2101 caaaagttgc tgaattagaa aataaaatta ttcccggaat gcccaaaaat gaacgcttaa
     2161 agattaagca aaaaatcaga aatattacaa aaaatgccaa tcgaaaagca aaaggagaag
     2221 agcatggtcg acgcggtcgt taaaactccc gaatttgtac cttttacttc tgtaggaata
     2281 ttccgttttg gtgcggatat aacacaatac aaaaacatac tggaaacttt tatgtatgaa
     2341 cctcctgatg agtttagaac agaatattat gaatctcccg actcaaattt actcattgcc
     2401 gtcaaaaaaa aaacaaaatt atttcgatat tttgttatca ggagctatat tttatgggcg
     2461 ttaatttaat aggtctaaat tttgaagatt ttaaacaact tttccatcac cctaaatcat
     2521 acggtgttga tgaatattat ttatcaaatg aatcttatcc gacatatgtc tatgagtttg
     2581 atgaaatagg ggtacaagca tgggaagcaa aagggaaagt tgttactatc attgccgggg
     2641 gtaaagacaa ttattcaaca gaaccttact acgatgaata acattacaga aaagaaacaa
     2701 gaaacggtac atttgataaa aacctaaatc gtattggaga ttaattatta tgtgtgagtt
     2761 caaggatttt agaagaaaca tcccttgttt tgaagagtat gacgaaaatt catttattgg
     2821 caaatggtat gatgacgggg tgtgggatga tgaagaatat tggaaattgg agaatgattt
     2881 aatcgaggtt agaaaaaaat atccttatcc gatggatata ccaagggata ttgtgattgg
     2941 aatcggtacc attattgatt ttttaatggt tccaaattgg gagctttttg aaattaaagc
     3001 ttccccttgg ttgcctgata gtgtgggaat tcatgaacgt tatgaaagat tcacaacgat
     3061 gctccgttat atttttaccg agaaagacat agtcaacgtg cgatttgatt attacaacaa
     3121 aaaatagcaa aacccggcaa tcaaaatgcc gtctgaagcg atatttgctt ttcagacggc
     3181 attttgatga atgaaacagg atattaagaa ctaagttctt caaaaatcct gcctccgtcc
     3241 cttatacgtc ggtgctttag tcaaaacggc agacggctga aaagcaaaca ctgtcatcaa
     3301 ccatttgaag accggatgga ataggaaaga aaatcggtaa aatctccacg attagacggt
     3361 aaaatacctc ggtgtcgctg aaaccctgat cgtcgtgttg ccaaaacaaa aatcagatat
     3421 ggaaaacgat agtgaagatt aaaccattac aattttctaa caatagtcgc agattttacg
     3481 tagacaaaat ttgtgttacc gaacaagatg tgaacatttt ggcatccgac cgaaattatg
     3541 aattaaatat tgaaatattt attgacaata taattcattt tcaaataaca gatgaatctt
     3601 ataaagtaaa attttcagaa tatttatttg aaaataaaga aaaaaatgat tgggatagaa
     3661 atcctgctat aaattatttt ttcgagataa tagatgacag ttatatggac tggttgaaag
     3721 aagaaagttt tggttttttt gaaaagaaat attataaggc ttacattttc ttttttagcg
     3781 attctgtaat agaagttatc agctcgacag aacctgtatt ttattcaaaa taacaaatta
     3841 tcaaacaaag ctctgattaa aaacccaaca atcaaaatgc cgtctgaagc ggtattcggc
     3901 tttcagacgg cattttctcc gattagcaca ggatgccggg gcttgccggc ttgattttgc
     3961 cgaaattgtt ggcacgcaat tgggaggggc ggcaacctat ggaaaataga tttgaaaaaa
     4021 tcgttaagga tatttttagt tttacccaat ttgtttccga agcagaaaat gagattccta
     4081 accaatcctc ccgttattac gactgttggt gggaattgga aatcttgaat gcttccgcat
     4141 tggaagattg ggaaatatcg ggaaagcctg aaaaatggga gaaatggaca gaaaaatatc
     4201 agaccgaagc gttacagtca gtcaagcatc tatttgccga agaagttcct aaaaataata
     4261 tagtttgcaa tatttctcat tttttgcatt ttgccgatcg ggcagaagcc gacattccca
     4321 atcaatcacg ttcatatgcc gaatattggt gggaaatgga aatgttcaaa atgtcggttt
     4381 tagaagattg ggaacgctcg ggaagaccgt taaattggga aaaatggcaa aggcaatatc
     4441 gatcttaagc atttgaatta gttgaaaatt tgaatcaaag caaacaagag taacaagccg
     4501 cagcctgcat aggttttttt aaagtacggc agcaagcgta gattagatta aaaattaaac
     4561 aatcaaagct cgtctgatat aatcgatata tcctgttaaa cgaattaaac gaaaaataaa
     4621 tatgtttaaa aaattcaaac tggtattgtt gtcatttttt gcccttgtat ttgccttttg
     4681 gctggggacg ggtattgcct atgagattaa tccgcgttgg tttttgagcg atacggcaac
     4741 tgaaatacct gaaaatccga atgcttttgt ggcgaaactt gcccgcctgt tccgaaatgc
     4801 cgacagggcg gttgtcatcg tgaaggaatc gataaggacg gaggaaaatc ttgccgaaac
     4861 tgtggatgac ggtccgttgc agtcggagaa ggattatctc gcgctcgcta tccggctcag
     4921 tcgtttgaaa gaaaaggcga aatggtttca cgtaacggag caggaacatg ggaaagaggt
     4981 ttggctggat taccatatcg gcgagggcgg tttggttgcg gtttcgcttt cgcaacgctc
     5041 gccggaagcg tttgttaatg ccgaatatct gtatcggaac gatcgtccgt tttctgtaaa
     5101 tgtgtacggc ggaacggctc acggagaaaa ttatgaaacg acaggagaat atcgggttgt
     5161 ttggcaaccg gacggttcgg tatttgatgc gtcggggcgc gggaaaatcg gggaagatgt
     5221 ttatgagcat tgcctcgggt gttatcagat ggcccaggta tatttggcga aataccggga
     5281 tgtcgcgaat gatgagcaga aggtttggga cttccgcgaa gagagcaacc ggattgcatc
     5341 ggactcgcgc gattctgtgt tttatcagaa tatgcgggaa ttgatgcccc gggggatgaa
     5401 ggcgaacagt cttgtggtcg gctatgatgc ggacggtctg ccgcaaaaag tctattggag
     5461 tttcgacaat ggaaaaaaac gccagagttt cgaatattat ttgaaaaacg gaaatctttt
     5521 tattgcacaa tcttcgacgg tagcattgaa agcggatggc gtaacggcgg atatacagac
     5581 ctatcatgcg caacagacgt ggtatttgga tggcgggcgg attatccgcg aagagaaaca
     5641 gggagacaaa ctgcctgatt ttcctttgaa cttggaaaat ttggaaaaag aggtgagccg
     5701 ttatgcagag gctgcggcga gacgttcggg cggcaggcgc gacctttctc actgaacgca
     5761 aatgccgtct gaaaccggtt ttcagacggc attggagagt ttgacgcttt ttgcaatgcc
     5821 gtatattcca aatccaattc gctacaaatt gaaaaccaca atccaaaaaa cggaaataca
     5881 cattaaacgg gtctttttac cgaccgtatc atcccatagg ttttccaccc ctttttttca
     5941 tccataaatt caaaccattt aagtttgcat ccttaccgac aattgcagca gcatcatccg
     6001 cataacggtc acccaaatta gccaaataac gccaaccgcc ataggtatcg ccacggaaag
     6061 cataaccacg caaggtttgg atttgttctg gtgttaaaac aacttcaccc tcattcataa
     6121 taagatcctt taaattattt tttacttgga ttgtttacaa aactctgtac caattgctct
     6181 acttgttggt gataacctac caactcctgc tgtgtgagct gctgcttttc ccgaataaac
     6241 gggtaatacc gtaccgtgaa accgttaata caggaaccta cgctagtgct ataattatct
     6301 tcttcagttt ggtttattcg attattccga tgagtaataa tcgccggatt ttctcctttt
     6361 ttaatatagt aaagtttatg aaaatatgga ttttcaggaa tatgtctttt atcttcataa
     6421 gcttcatatt caggataggc agatggcatt tttttaaccc cgtaagtaag caatctttct
     6481 cccatagtcg tgcttaaact acacgcatct tttcggataa caatatcgtc cacgctatcc
     6541 aaaccgtcgc gtagaaattg gataaagtgt gctttgtttt gctggttgta tggctcaaga
     6601 acccagcctt ggtcggtttt tttgtaatag acgctaatct ctatcaaatt aatggagtaa
     6661 gcatcctgcc cgggcgtgtc agaaagccat tttgtggcat tgtcttgcgt ataccgtgca
     6721 agatagccat a
//
LOCUS       DAWFLT010000102         6711 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWFLT010000102 DAWFLT010000000
VERSION     DAWFLT010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.102"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(372..932)
                     /locus_tag="WGR74_001926"
     CDS             complement(372..932)
                     /locus_tag="WGR74_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ0906278.1"
                     /translation="MNMKTLLALAVSAVCSVSVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
     gene            complement(1349..2389)
                     /gene="mutY"
                     /locus_tag="WGR74_001927"
     CDS             complement(1349..2389)
                     /gene="mutY"
                     /locus_tag="WGR74_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ0906279.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVEQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPTHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2552..3656
                     /locus_tag="WGR74_001928"
                     /pseudo
     CDS             2552..3656
                     /locus_tag="WGR74_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            complement(3679..3897)
                     /locus_tag="WGR74_001929"
     CDS             complement(3679..3897)
                     /locus_tag="WGR74_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906280.1"
                     /translation="MPSEALSSLRRHFAAWRRSRHTRQHHSDILPLKCPSAFRIIKIT
                     FSSFDSSRTAHTTRLKKCRLKRIQTASA"
     gene            complement(3912..4550)
                     /locus_tag="WGR74_001930"
     CDS             complement(3912..4550)
                     /locus_tag="WGR74_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ0906281.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     HNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(4731..6566)
                     /gene="edd"
                     /locus_tag="WGR74_001931"
     CDS             complement(4731..6566)
                     /gene="edd"
                     /locus_tag="WGR74_001931"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ0906282.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIHSAKQMGRLERN
                     QLGCSNLAHGYASMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAIGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRDELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKAGNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVSGELNVLINETE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
BASE COUNT         1508 a         1599 c         1889 g         1715 t
ORIGIN      
        1 gccaacaggc taagtgcgcc cgctgccgcc taaaaggcag cccggatgcc tgattatcgg
       61 gtatccgggc aggattaagg gggtatttgg gtaaaattag gaggtatttg gggcgaaaac
      121 agccgaaaac ctgtgttggg atttcggctg tcgggaggga aaggaatttt gcaaaggtct
      181 caaatagtac ggaatcgatt cacttggtgc ttcagcacct tagagaatcg ttctctttga
      241 gctaaggcga gccaacgttg taccggttta aatttaattc actatactgc tgaagtcgtg
      301 aattaaatgc taaatatcta gcaaaaaagt gcggtcaaaa tttaccgcac ttttcgtttc
      361 gaacaatcga attatttaat cacgccacat gccatacgtg ggccgccacc gccaagtgga
      421 gctggatgat cggagtgatt atcaccaccc gtgtggatca taatagagtg accgcgaaca
      481 tcatctaaat gtttaagacg tggtgctaaa acaggatttg ttgctgtgcc atcatgcaat
      541 acagttaatg caggtaaatc acctaagtgt gcatcatctt gccatgggta accatgttgt
      601 tttgcacctt taggatccca gtgaccgcct gcgcctaaac cagctgtcaa tttaccttct
      661 ttttcttttg gctcacagct tgggttttca tggatgtgga aaccatgtaa gccttcgctt
      721 aatccttgta aatcaggggt aaacacaaga ccatagttag attcagtaat agtcactgta
      781 cccacatctt tgttaccgtt tactggatca agttgttgca ctttcacttc aatagaagca
      841 cctttaggta tcgtattatg ctcgtgtgct tgcgcaacac taactgaaca tactgcacta
      901 accgctagtg ctaataaggt tttcatattc attgcaatct cctttattgc agtaaaaact
      961 cgcgactatt ttagtcaatt ttgttttaaa agccgataaa tttttacgaa aattttacat
     1021 taattgttat ttgcagaaaa ttagaacccg ttatcacgtc ttggataaat gccaaaacca
     1081 ttgtgattca gagaattaaa aagaaaaatt tcggaatcct catccttttc tgaaacctga
     1141 ttgcggtatc catggctttg tctttgtcat ttaagtttgt gttcatgttt ttgaccttct
     1201 ttgcttttta aatccttttt aaattgccga cttcttccgt tccaaatgtc ccatttgaac
     1261 attatcccgc cgcctaaaat acgacccaaa ttcatcggtt gttcttgctg cgatacctgc
     1321 aaacaacagt tcaatgggtt tattttgttt attctaacct attaccgttt aaataaattt
     1381 ccaaaggctt gggcaggccg taatctttca aatgcgtcgg ctttatccaa atgccgtctg
     1441 aagggctttc agacggcatt tgtccttcaa acggcgtaat catcagcaac cggtgcgtca
     1501 gccggtgggt cagggcggtt tgttcgtcca tatctgccat ggtcagggag aatttggcgg
     1561 caaagtcgga aagcccgttc aaactttcaa aacacggcac gcaatacagc ccgccccaaa
     1621 tgcctttggc ggggcgtttt tccagcaaaa tcgcgccgtc ccggttgcgg acaatcagcc
     1681 agtaaagcgg cagggtcggt acttcggcgg cggttttttt gcgcggcagc tcggcggtgc
     1741 ggttttgctt tttcgcttcg cagatgtccg ccatcgggca ttggtggcac aagggtttcg
     1801 tccgtttgca cacggtcgcg cccaaatcca tcaaaccttg tgtataggca ggcatatcgg
     1861 cgttttcaga cggcagcagg ctttcggcaa gtgtccagag cgagttttca aattttttgt
     1921 cctgcggatt gccgtcgcgg gcgaacacgc ggcagagtac gcgtttgacg ttgccgtcca
     1981 aaatggtttc gcggcggttg aaggagaagg cgcaaatggc ggcggcggtg cttctgccta
     2041 cgccgcagag ggtttccaag tctttgcgct ccgacggaaa cgtgccgccg aattgttcga
     2101 cgacttgttg cgcggctttg tgcaggttgc gcgcgcggct gtaatagccc aagcccgccc
     2161 acaacgacaa cacttcgtct tgcggcgcgg cggcaagcgt ctgaacggtc gggaattttt
     2221 ctaagaaacg cggatagtag tccaacacgg tggcgacctg cgtttgctgg agcatgattt
     2281 cggaaagcca gacgcaataa gggtttttga cctgccaagg gaggtggtgg cgaccgtgtt
     2341 gtttttgcca gcggatgagc cgttcggaga aggggatggg tgtgttcatt aatatcaatc
     2401 ggtggtttta tttatattta aaacagtatg ttattgtata acattgtgaa aataattctt
     2461 attgacttat tttttgtagg ggcatataac tcatataagg aaactttatt gcggtattga
     2521 aattatttat caacaagcaa ggagtatcag catgaaagca atggtttatc acggcgcaaa
     2581 cgacatccgt tttgaagaaa aaccccgtcc gcagattatc gatccgaccg atgcggtggt
     2641 gaaaatcgtc aaaaccacga tttgcggtac cgatttgggt atttggaaag gcaaaaaaac
     2701 ccgaagtcgc cgacggccgt attctcggtc atgaaggcat cggtattgta gaagaagtcg
     2761 gcgaggctgt aaaaaacatc aaagtcggcg ataaagtcat tatttcatgc gtcagcaaat
     2821 gctgtacttg cgacaactgc aaaatccaac tttattcgca ttgccgcaac ggcggctgga
     2881 ttttgggcta catgattgac ggcacgcagg cagaatacgt ccgcacgcag gcagaatacg
     2941 tccgcacgca ggcagaatac gtccgcacgc aggcagaata cgtccgcacg ccttatgccg
     3001 acaacagcct cgtgccgctg cccgacaacg tcaacgaaga agtcgccctg ttgttgagcg
     3061 acgctttgcc gaccgcacac gaaatcggcg tgcaatacgg cgatgtcaaa cccggcgaca
     3121 ccgtattcat cgcaggtgcc gctcctgtcg gtatgtccgc cttgccgacc gcccaactgt
     3181 acagccccgc cgccatcatt gtttgcgata tggacgaaaa ccgtttgaaa ctggcgaaag
     3241 agttgggcgc aacccatacc attaaccctg cttccggcga tgtaagcaag caagttgccg
     3301 ctatcgtcgg cgaagacggc gtggactgcg cgatcgaagc cgtcggtatc cctgcaactt
     3361 ggaatatgtg ccaagacatt gtgaaacccg gcggtcatat cgccgttgtc ggcgtacacg
     3421 gtcaatccgt tgatttcaaa ctggaaaaac tgtggattaa aaagcttgcc atcactaccg
     3481 gcctggtaaa cgccaatacc accgaaatgc tgatgaaggc aatttccagc agctccgtcg
     3541 attacaccaa aatgttgacc caccgtttca aattcagcga attggaaaaa gcctacgacg
     3601 tgttcaaaca cgccgccgaa aatcaagcga tgaaagtggt tttggaagcg gattaatcgg
     3661 cgttgaatca gttgtttttt aagcggatgc cgtctgaatg cgtttcagac ggcatttttt
     3721 cagccgcgta gtatgggctg tccggctgct gtcgaatgaa ctgaacgtta tttttattat
     3781 gcggaaggcg gatgggcatt ttaggggcag tatgtcggag tggtgctgcc gtgtatgccg
     3841 actgcgccgc caagcggcaa aatgccgtct gagactggaa agggcttcag acggcatttt
     3901 tacgatgcga ttcaggcttt gggtttcaat gccgccgctt ctttggcgag acgggtgatg
     3961 gtgtcccagt ctttgttttt cacggcttct ttcggcgtca gccatgagcc gccgacgcac
     4021 aggacgttgg gcagtgccaa gtaatcgggc gcggtggcga ggctgatgcc gcctgtcggg
     4081 cagaagcgaa catcggcgta agggccgtac agggctttga gcatggcttt gccgccaacg
     4141 acttcggcag ggaagagttt gagggtgtcg atgccgtgtt ccaaagccag ttggatttcg
     4201 cccggggtgg caacaccggg aatcaggggg atgccgctgt tgtggccggc tttggcgagg
     4261 gattcgtgca aacccgggct gatggcgaaa accgcgcctg cgtcttcgac ggctttgagc
     4321 tgttcagggt tggtaaccgt acccgcgccg acgatggcgt tgggcacttc tttggcaatc
     4381 aggcggatgg catcgaggcc gacaggggtg cgcagggtga tttcgagggt ggggatgccg
     4441 ccttcgacaa gggcgtggga caaatcgacg gcggtgctta agtcgtcaat cgccattacc
     4501 ggcacaactg cgccggcggt caaaatttcg cggggggtca gtttggacat ttcggttctc
     4561 cgggtggaat ggggtatttt attaagatgg gacaggtcgt aggtcggatt caagaaaccg
     4621 acatcttgga cggctgtgtc ggattcgaga atccaaccta cgtctatctt aaaacacttg
     4681 aataattttt cagacggcct tcggtctgaa aaggccgtct gaaacgcgca tcaggcaaat
     4741 tcgccgccga aactcatggc accggtttcc gcgctgctgg tcatgctgcg gaagttggcg
     4801 aagagttcgc ggccgcagcc ttgttggttc gcgcccaagt cgatgctttc gacttcgcgg
     4861 gcattccatt cggtttcgtt aatcaggacg ttgagttcgc cgctaacgga gtcgaagcgg
     4921 atcaggtcgc cggtacggat tttggcgatg ttgccgccca tcagggcttc gggtgtcatg
     4981 tggatggagg ccggaacttt gccagatgcg ccggacatac gaccgtcggt cagcagtgcc
     5041 actttgaagc cgcggtcttg caggatgccc aaaggcgggg tcagtttgtg caattcgggc
     5101 ataccgttgg cacgcgggcc ttggtagcgg acgacgcaga cgaaatcgcg ttccaactcg
     5161 ccgcgttcaa atgccgccaa cacttcgcgt tggtcgttga acacgatggc aggcgcttca
     5221 ataatgcggc agccttcgcg cacggcggac actttaatca cgccgcgtcc gatgttgcct
     5281 ttcatcaggc gcagaccgcc gtcgggggag aacgggttgc cggctttgcg caggatgtcg
     5341 tcgttgccgc tggtttcggg ggcttcgcgc cattcgagtt tgccgtcgat aaggaaaggc
     5401 tctttggtgt agtggcgcat accgtgtccg acaacggtat cgacatcgtc gtgcaacagg
     5461 cctgcattca gcaattcgcg gataacgaaa ggtaggccgc cggcagcggt aaagtggttt
     5521 acgtcggctt tgccgttggg gtacacacgg atcagcaatg ggatgatgga agaaatttcg
     5581 tcaaagtcgt cccagttcaa aatcacgccg gcggcgcgtg ccatggcaac gaggtgcatg
     5641 gtgtggttgg ttgagccgcc ggttgccatc aggccgatta gggcgttgat gaaagatttt
     5701 tcggtcaaca tttcacccaa tggtttaatc gtgccgtttt tgatgccgcg cgcgaggtgt
     5761 ccggcggcat agcgggtcag ggcttcacgc aggtcggtgt aagggtggac gaaggcggcg
     5821 gcaggcaggt gtacgcccat catttccatc atcatttggt tggagtttgc cgtgccgtag
     5881 aaagtgcagg tgcccgggct gtggtaagaa cccatttcgc ttttcaaaag ttcgtctcgt
     5941 ccgactttgc cttcggcgaa aagctggcgg gtgcgggctt tttctttgtt gccgatgccg
     6001 ctggacatcg ggcctgcggg gacgaagata cccggaatat gaccgaacga aagcgcgcct
     6061 atcatcaaac ccggcacgat tttgtcgcat acgcccataa acaggctgcc gtcaaacatt
     6121 tgatgcgaca gaccgatagc ggtactcatg gcgatcacgt cgcgggaaaa cagcgacaat
     6181 tccataccgg cgtagccttg cgtgatgccg tcgcacatgg cgggtgtgcc gccggcgact
     6241 tgggcggtcg cgccgttttt ctgcgcttcg tctttaattt ggtcggggaa gtctttaaac
     6301 ggctggtgtg cggaaaccat gtcgttgtag gcggtgatga tgcctaagtt ggggacggtt
     6361 tcctggagca tttcgatttt gatgcttttg ggcatagagg catagccgtg cgccaagttg
     6421 gagcagccga gctggttgcg ctctaagcgt cccatttgtt tggcactgtg gattttcgcc
     6481 agatattttt cacgcgtcgg acggctgcgt tcaataatgc gcccggtgat ttcggcgagt
     6541 ttggagtgga taggagtagt gttcacggtc ggtctcctgt cggaacgggg ggtattgggg
     6601 atttgtttgt gcgtttctga ttttttgtat tgggtgtggg gcatttggcg tttcagacga
     6661 cctttcaaac cttacaaggg gtcgtctgaa aacctcacta tagtggatta a
//
LOCUS       DAWFLT010000103         6553 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWFLT010000103 DAWFLT010000000
VERSION     DAWFLT010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.103"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(146..1501)
                     /locus_tag="WGR74_001932"
     CDS             complement(146..1501)
                     /locus_tag="WGR74_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ0906283.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSLLENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPTRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGAGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARFERQTQALQHYRPDVSVCKNNIVRLQTALPAAFSQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            1698..2519
                     /gene="nadE"
                     /locus_tag="WGR74_001933"
     CDS             1698..2519
                     /gene="nadE"
                     /locus_tag="WGR74_001933"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ0906284.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRPTLLLDMPIRQHPDQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGVHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(2589..3062)
                     /locus_tag="WGR74_001934"
     CDS             complement(2589..3062)
                     /locus_tag="WGR74_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0906285.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAELLAYMQNELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTDNFQAPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            3365..3697
                     /gene="trxA"
                     /locus_tag="WGR74_001935"
     CDS             3365..3697
                     /gene="trxA"
                     /locus_tag="WGR74_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ0906286.1"
                     /translation="MSSELIVHTGDAAFEQDVLKSDLPVLLDFWASWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGDVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(3750..4697)
                     /locus_tag="WGR74_001936"
     CDS             complement(3750..4697)
                     /locus_tag="WGR74_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0906287.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAV
                     DVYGDQIHLQEYDTGWLMRSEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRQFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            5025..6413
                     /locus_tag="WGR74_001937"
     CDS             5025..6413
                     /locus_tag="WGR74_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0906288.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFARLEVLILDEAD
                     RMLDMGFIDDIETIVEATPEDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDADIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGWRGDHKPSKEGFGGKTRGEGFKKEGFKRDGFKKTGEGFKGKRKAGDS
                     FAGKGERRYKDR"
BASE COUNT         1566 a         1765 c         1725 g         1497 t
ORIGIN      
        1 aacgtatcaa tcgtcattcc cgcgcaggcg ggaatacggt tttttgaatt tcagtcattt
       61 ctgataaacc ccggcggctt tccgcttccg gactcccgct tttgcgggaa tgacggcgga
      121 gggggagcgg tttttccgat tgggtttaaa tgcaatcgaa caaatcctgc tgcccttgtt
      181 ctttgcttac gcgcacgtcg gtttcgccgt cggcgaaggt aatgtgcagc ttctgcccct
      241 gcttcaaaac atcggcgttg cggatgactt gtccgcgtgt gtttttgacg acggaaaagc
      301 cgcgctccag aatgtgctgc ggcgaaacgg cttcgagcaa tgcggcttgg gcggtcaggc
      361 tttggcggcg gtgggtaagc agttggctga aggcggcggg caatgccgtc tgaaggcgga
      421 cgatgttgtt tttgcaaacg gaaacatcag gacggtaatg ttgcagggct tgggtttggc
      481 gttcaaaacg ggcggtgtgg gcacggaggt tttgcttcat cgagtaagac agcgtttgcg
      541 ccagcttgcc gattgaagcg cgctgctcat caagtttttg gcgcggatga cggatttgcc
      601 gcgcaagcca gtcgagtttt tggctggcat cgaaatagcg ttgttccaaa acggttttca
      661 gacggccttg cgcctgagcg agacgatgta gcgattcttg gcggttgggg ctgaccagct
      721 ccgccgcacc ggtcggcgtg ggcgcgcgca cgtcggcgac aaaatcggca agcgtaaaat
      781 cggtttcgtg tcccacgcca ctgacgacgg gaaccgtgca ggcttcgatt gcgcgcacga
      841 ccggttcttc gttaaacgcc cacaagtctt caatgctgcc gccgccgcga cagacaatca
      901 acacgtcgca ttcggcgcgt tgcgaggcgg ttttaatcgc ttgggcaatt tgtaactcgc
      961 tgcctgcgcc ttgaacaggt gtcggataaa cgataacggg aatttcgggc gcgcggcgtt
     1021 tcaaggtggt aacgacatcg cgcaatgccg ctgctgccag gctggtgacg atgccgatac
     1081 attgcggacg ggtaggcaac ggctttttgc gttccgccgc aaacgcgcct tccgcctgca
     1141 actgtgcctt caaccgctcg taggcttcgt aaagctgccc caaacctttg agccgcacct
     1201 cattcacggt aatttgaaat tcgccccgcg cctcgtaaat actgattttt cctgatactt
     1261 cgatatggtc gccttctttc aaaggcttcg ccaaacgcgc cgccgcacct ttgaacatcg
     1321 cgcaacgcac ctgcgcgcgg ctgtctttga gcgagaaata ataatgtccg ctggcggcac
     1381 gggtcaggtt ggacacttcg cccgcaatcc acaggccggc aaggtggttt tccaaaagac
     1441 ttttggcaaa tgcgttcaac tcagaaacgg acaacacgtc agaatgaaaa aaatcagaca
     1501 tagaatcaat caaataataa aaaatatgaa tatgttttga agcctaaggc ggtattagtc
     1561 cgcctaaatt gtcaacaata ttataacacg cgccatcttg ccgcccgcct tttcccgtat
     1621 gactttttta agcggggaat gggaaaaata ttcatcaacc cgccctcaat ctattcaaat
     1681 tacaccgccg acaagctatg atgcggatat tttcgacagg aggaaaaatg gatacgcagg
     1741 cagttatcac acatattgtc cgatggttgg acgaatacgc cgcccgggca aatgcaaaag
     1801 ggtttgtcgt gggcgtttcc ggcggcatcg attccgccgt cgtctccgca ctcgccgccc
     1861 gcaccggccg ccccacgctg cttctggata tgccgatacg ccaacacccc gaccagcttg
     1921 agcgggcaag gctgcacatc cgcaatctgc aacggcaata tgccaatgta agcgcacaaa
     1981 cggtcgatct gaccgacacc ttccagacct ttgaacaaac cgtcggcgtt catcagacgg
     2041 catttgacaa tcagccgctt tccctcgcca acgccagaag ccgcctgcgt atgctgaccc
     2101 tgtactacta cgggcagata cacggactac tggttacggg gacaggtaac aaaattgaag
     2161 atttcggtgt aggatttttt accaaatacg gcgacggcgg cgtggacatc agcccgattg
     2221 ccgacctgac caaaacccag gtttaccggc ttgccgaagc attgggcgtg gacgaggcga
     2281 ttcaaaaagc cccgccgacc gacggcctgt gggatacgga acgcaccgac gaagaacaga
     2341 tgggcgcaag ctatcccgaa ctggagtggg caatgggcgt gtacggcacg cgcaaacccg
     2401 aagattttga agggcggcag cgcgaagttc tggaaatcta tacgcggctg caccgcgcga
     2461 tgcagcacaa aatcaacccg attcccgtgt gccgcattcc gcccgaattg ttgggctgaa
     2521 acgtggaaat gccgtctgaa gcggaaaacc gtgtttcaga cggcattgac atatccggct
     2581 cctatccctt aagaatcgag tacgcgggca aacaaaatat cgttttccaa atgaatgtgg
     2641 tcgttcaaat cctccaccat ttctttggcc aaagcgtaaa gccgcgtcca gcttccgcag
     2701 gctccttcgg gcgcttggaa attatcggtc agctctttga gccttgcgat ggcgcggtcg
     2761 tgttcttcat gttcgtgcat catcacgctg atgggcatcg ccgcaccccg accgacaccc
     2821 tgattaatca tcggaaacag catcctttcc tctttcatca tatgcatcag cagttcgttc
     2881 tgcatatagg caagcagctc ggcaatttcc gccggaaagg tatcggcatg aacttgggcc
     2941 actttttgcg ccagcggcac caattcttca aattgtgcac ggtgtacatt gtggtagcgt
     3001 tgcaggatat gatcgacggt tgcaccaaag ggagcagttt cccaaacgga aaaatcagtc
     3061 atggcagggt tccttttatt tatagggttt cgggtttggt tttgaacatt catactttaa
     3121 gaatctattt aaacggagca tacaccgccc gtgcgtttct gtacagcttc aaaaacattc
     3181 cttacatttt gataataaaa gtaattttca aaaataaaat actgtccgaa ccgttttttt
     3241 agaatttgca aaggcgattg gggcggtaca gaaaaactat tatcccgccc gcccacttga
     3301 aatttttatg cccaagccct atcctgcacg ctatcgtgcc aatcccaacc gaaaaggaaa
     3361 aataatgagc agcgaattga tcgtacacac cggcgatgcc gccttcgagc aagatgtttt
     3421 gaaatccgac ctccccgtcc tgctggactt ttgggcttcg tggtgcggcc cgtgcaaaat
     3481 gattgccccg attttggacg acattgccgc cgaatttgaa ggccgtctga aagtagtcaa
     3541 aatcaacatc gacgacaacg aagccacccc gtcccgtttc ggcgtgcgcg gcattccgac
     3601 cctgatggtg ttcaaaaacg gcgacgtcgt cgccaccaaa gtcggcgcat tggcaaaagg
     3661 tcagctgacc gcctttgtcg aagcctctat cgcctgataa agcgcaatcg aaaaagccgc
     3721 cggaagattc cggcggcttt ttcgcaccct taagatttgt ggcggatttc ccagcaccga
     3781 tggatttttt tgttgcggaa atcttcggga acggattgtt tggaaatgtc tttgacggcg
     3841 tattgttccg ataccaagtc gtctaagacg aagctgcgca ggttgttgga aaagtacaaa
     3901 atgccgtctg aagcgagcag cttcaccgcg ccgtcaatca gctttttgtg atcgcgctga
     3961 atgtcgagga tgtcggacat tttcttgctg ttggaaaaac tgggcggatc catcacaatg
     4021 aggtcgaact gcctgccttc cccatatgcc gtctgaagat attggaacac gtcggcgcgg
     4081 acgattttgt gccgttccgt atcgatgccg ttcaattcaa aattgcgtct cgcccaatcg
     4141 agataagtgt tggacaaatc gacagtttcg ctggatgccg cgccgccggt ggcggcatag
     4201 acagtgaagc tgccggtgta ggaaaacagg tttaaaaaac gtttgcccgc cgccgtttcg
     4261 ccgacttttt tgcgcgtgtt tcgatgatcc aaaaaaagcc ccgtatccaa atacttatca
     4321 aggttgaccc aaaacttgcg gccgttttcg gtgatgacga aatcgtcgcc cgccttgccg
     4381 gttttttcgt attgctgcaa acctttttgg cgttcgcggc gtttgaggcg gatttgttcg
     4441 ggcgcaaaac cggtaacgaa ggcgacggct tccaagactt cggcaagcca cgcttcgtat
     4501 tcttcggacc gcatcagcca gccggtatcg tattcctgaa ggtggatttg gtcgccgtaa
     4561 acatcgacag caaaggggaa ttgggggatg tcgcggtcgt aaatgcgcca ggcttcgatg
     4621 ccgttgcgtt tcgcccattt cataaggtgt ttgatgtttt tgcccaagcg gttggcaaac
     4681 ggtgtgatgt cggtcattgg tttcaggcgg aataaagtgg aaaacggcaa ttttactgta
     4741 attaacgccc gattgcttga ccgtttcggg caaaccctat accatccgtc gcttatcttg
     4801 tcatacgaag ccatcgcctt ccaacctaaa ccgcccttac gggcgcgttt cttctgttgc
     4861 tttgattttg caaagcgtat ctgtgcaggt tgccgtcgat gtaaaccaca agcaagccgc
     4921 ttgcgacaac cctgtaactt cacattcccc gtatcgttac ccttccctgc ttcaggccgt
     4981 ctgaaccttt cggacgcgag cgttgttgtc ttccaaggat agccatgtct attaaatttg
     5041 ccgatttgaa ccttgataaa aacattttgt ccgccgtcag cagcgagggt tacgaaagcc
     5101 cgacgccgat tcaggcgcaa gccattccgt ttgctttgga aggccgcgac atcatggctt
     5161 cggcgcaaac cggctccggc aaaaccgccg cctttctgtt accgactttg cagcgactga
     5221 ccaaacgcag cgaaaaaccg ggcaaaggcc cgcgcgcgtt ggtgttgacg ccgacccgcg
     5281 aattggcggc tcaagtcgag aaaaacgcgc tggcgtatgc caaaaatatg cgttggttcc
     5341 gtaccgtcag catcgtcggt ggtgcatcgt tcggctacca aacccgcgcc ctgagcaaac
     5401 cggtcgatct gattgtcgcc acgccgggcc gtctgatgga cttgatgcaa agcggcaaag
     5461 tcgatttcgc ccgcttggaa gtgctgattt tggacgaagc cgaccgtatg ttggatatgg
     5521 gctttatcga cgacatcgaa accatcgtcg aagccacgcc ggaagaccgt cagactttgt
     5581 tgttctccgc cacttgggac ggcgcggtcg gcaaactggc gcgcaaactg accaaagacc
     5641 ctgaaatcat cgaagtcgaa cgcgtggacg accaaggcaa aatcgaagaa caactgctgt
     5701 actgcgacga tatgcgccac aaaaaccgcc tgctcgatca tatcttgcgc gatgccgaca
     5761 tcgatcaatg cgtgattttc acgtccacca aagccatgac cgaagtcatc gcggatgaat
     5821 tgtacgaaaa aggtttcgcc gccaactgcc tgcacggcga tatgccgcaa ggctggcgca
     5881 accgcacgct gatggatttg cgcaaaggcc gctgcaaaat tttggttgcc accgacgttg
     5941 ccgcacgcgg tatcgacgta ccgaccatta cccacgttat caactacgac ctgccgaaac
     6001 aggcggaaga ctacgtccac cgcatcgggc gcaccggccg cgcaggccgc acgggtattg
     6061 cgattacgtt tgccgaagtg aacgaatacg tcaaagtcca caaaatcgaa aaatacatta
     6121 accgaaaact gcccgaactg accatcgaag gcatggaacc gacccgcaaa cgcaaatccg
     6181 caggcggcaa gccgaaaggc aaaggcggct ggggcgatcg taaatccggc ggttggcgcg
     6241 gcgatcataa accgagcaaa gaaggcttcg gcggcaaaac gcgcggcgaa ggtttcaaga
     6301 aagaaggctt taagagagac ggtttcaaaa aaaccggcga aggcttcaaa ggcaaacgca
     6361 aagccggcga ttcttttgca ggcaaaggcg aacgccgtta caaagaccgc taagccccaa
     6421 cctgccgcat aaaccaatgc cgtctgaaac cgatttcgag tttcagacgg catttttaca
     6481 atgtttcagc accgcccggc tttgataccc aaaggattaa tctgcaatat agtggattaa
     6541 caaaaaccag tac
//
LOCUS       DAWFLT010000104         6234 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWFLT010000104 DAWFLT010000000
VERSION     DAWFLT010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.104"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..69)
                     /locus_tag="WGR74_001938"
     CDS             complement(<1..69)
                     /locus_tag="WGR74_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0906289.1"
                     /translation="MLPSKYEDNALQIKEKSTERTAP"
     gene            complement(211..2217)
                     /locus_tag="WGR74_001939"
     CDS             complement(211..2217)
                     /locus_tag="WGR74_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ0906290.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLAGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            2506..4533
                     /locus_tag="WGR74_001940"
     CDS             2506..4533
                     /locus_tag="WGR74_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ0906291.1"
                     /translation="MSLSEFIERRTSFNLMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEIIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(4625..6178)
                     /locus_tag="WGR74_001941"
     CDS             complement(4625..6178)
                     /locus_tag="WGR74_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ0906292.1"
                     /translation="MNRTYANFYEMLTAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTGQSRPDGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKTKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
BASE COUNT         1282 a         1456 c         1887 g         1609 t
ORIGIN      
        1 tggagcagtt ctttctgtac ttttttcttt aatttgcagt gcgttatctt catatttcga
       61 gggtaacata tctgctaatc tagtacagcc ccttttaaaa tgccgtctga accacgtttc
      121 ggttcggacg gcatttttgc atccttttat ggggatttcc cgcatcaggg cggtattcgg
      181 ggcgaatccc gcttcagacg gcattgccgg tcaaggcttg tccgaaggag gatgtccctg
      241 ttctttggcg ggcgggagga aaagtatgca gggattgctt ttaatctgat tccaaaaact
      301 cttaatcaga ttgcggatgc tgccgatttt cgtgccgcgc gagcgcaggg cgacgaagag
      361 ggcgaggctg aagctgacgc agaggttgac caggccgatg gcgaggacgc tgaaaatgcc
      421 gagtacgaac gtgcccaaac cgacgttgcc gctgacggcg gcatagccga ggttggcgga
      481 ggaaaaggcg acgtggcgga tgtccagcgg cagcccgagg aggtgtccga aatagccggt
      541 catacccaag agcatcccga agatgaagtt gcctgccaac gagccgtagt gtttgtggat
      601 gtaggcggcg aggacgcggc ggggcccggg gcgcatgatt ttacgcagca aggggttgaa
      661 gggcaggcgt tggcgcaggt tgaggtagtc ggcgcggttg tcgaaaaaac ctgcgatgat
      721 gccggagcag aacagccaca gacctgcaat ggcggcatac cacagcgtcg ggtaagcgat
      781 gatgtctatg gatttgaact ggtaggcggc ggtgtgggca tcgagtatgg gctgccggta
      841 cagatgggca tagccgaacg atatggcgca cgccaaaagg atggcgatgg aaacgttgcc
      901 gaaaacggcg acactttggg aacggcatac gtcgatgagg agcttggcga gtttgttgtc
      961 caccgctttg ccgccttcgt tgagatcgac ctgttcggca aagctggcgg cagtcatcgc
     1021 gggctgtttg gtggcgacgg tgcagtgcag catatggatg atcataaagc cgatgccgta
     1081 gttgaaccca gccgacaagg aagtcaggaa ggggctgagg ccgagtgtgc cgatgcggat
     1141 tttgagcagc gccatcaggg cgatgatgat gccgccgcct gccgccgagt agaacatttt
     1201 gaaatattct ttgcggttgc gggtaatgta gtgttcgccg tggtggcttt tgttttcggt
     1261 aatgctgcgg gcgagcatac ggatgctgct gcggcgcagg tggcgggtgc tgtattgttc
     1321 gaccgccgcg tagatgaggg agttcatcag cgcgatggtc aggcggttgc tgttgccggc
     1381 gccggtttgg atgtcggtga gcagcttcag acggtctacg gtctgccgga gccgttcgag
     1441 caggtgggcg actttgaccg acgaaccgga gccggcgccc gtccctttgc ggcgcaaata
     1501 gtcaatctgg ctgaaacatt ggtcgaacat cacttcgagg tgggcggtgt cgtaaggcgt
     1561 ggtgccgttg cggtagtgtt cgaccagttt ggcggtttcg cgttggaggg cgacgaagga
     1621 agaatcggct tccagcaggc gcggggcgat gcggatgagg tcgggttcga tggcttcgga
     1681 tgccgtccag acagacagca tttcgatggc gcgcaaacgc gcatcggcaa ggcggatgcc
     1741 cgccgtctgc aatttggacg gctcggcgtg gctccggatg agttcgtata aaaccagcca
     1801 ttggcggata ttgagcgttt gcagccattt gtcgtcgttt tcggaatgga aaagataaag
     1861 gaagacttcg cccaagttgg caaaatcttt atatgacggg ctgaagcgtt cgtagatgcg
     1921 tatgcccatt tcccgggcaa agctgtggcg cgagaagatg ccgagtttga tgagtgccgg
     1981 ataaatgtgt atttgcgcga gccagatgta aaaacaccgg ctgaagcggg acagcaattc
     2041 cctgtcttgt ttgaatgtgt cgataatcag gtcgaaacgt tcccccgcac attttttgcc
     2101 gccacggcgc aaaaatttaa tcagtgcgtt gaggacgttg acaaagtcgg tatgtcccaa
     2161 gctttccgaa agcagggggc gcaggttttg aggggtaatt ttcttcatat cagccattgt
     2221 accgcatcgg caatccggcg ttaaacgccc cgatggcggg gagggcgcgc ctgcggaagg
     2281 cggcgcattt tccgccggtt tgaaatcaaa ctatattaat gatataataa atatcgataa
     2341 atcaatctga tagaataatc cgtattgatt tttaattttt tcgattataa caatttcggc
     2401 cggtttgtgc atttcgtcat ccggaagccg gtattttgcc ggcaggtttt gaccgctttg
     2461 cccgatgacc gggtttgtct gtttttaaca aaggagtgag aaggtttgtc cctgtctgaa
     2521 tttatagaac gccgaacgtc atttaatctg atggttattt tgacgacttt gttttttgtg
     2581 tgtgttttgg tggtattggt tttaaccgtg ccggatcagg tgcagatgtg gctcgatcgg
     2641 gcaaaagaaa tcatttttac cgagttcagc tggttttatg ttttaacgtt ttccattttt
     2701 ctgggtttcc tgctgatact ctcggtcagc agtttgggaa acatcaggct cggacgggat
     2761 gaagatgtgc cggaattcgg cttcctgtcg tggctggcga tgctgtttgc ggccgggatg
     2821 ggcgtgggtc tgatgttttt cggcgtggca gagccgttga tgcattattt ttcggacatt
     2881 acggccggca cgccggaaca caggcagcag caggcattgc tgcacacggt gttccattgg
     2941 ggcgttcacg cttggtcggt gtacggtacg attgcattgg ctttggctta tttcggtttc
     3001 cgctacaagc tgccgcttgc cctgcgttct tgtttttacc ccctgttgaa agaaaaaatt
     3061 tccggaaggt tcggcgatgc cattgatatt atggcgttgc ttgctacttt tttcggcatc
     3121 atcaccacat tggggttcgg ggcttcgcaa ctgggcgccg gattgcagga aatgggctgg
     3181 attgccgaaa acagcttcag cgtgcaggtt ttgattatcg ccgccgtcat gtccctcgcc
     3241 gtcgtttcgg caatatccgg cgtggggaag ggcgtgaagg tgttgagcga gttgaacctg
     3301 agccttgcgt ttttgctgct gttttttgtt ttggcggcgg gtcccactgt ttacctgttg
     3361 tcggcattcg gcgacaacat agggaactac ctcggaaatc tggtgcgcct cagttttaaa
     3421 acttatgcgt acgaacggga acacaagccg tggtttgaat cttggacggt gctttattgg
     3481 gcgtggtggt gttcttgggc gccgtttgtg ggtttgttta tcgcgcgcat ttcaaagggg
     3541 cgcaccatcc gcgagtttgt cttcggggtt ttgctcatcc ccggcctgtt cggcgttttg
     3601 tggtttaccg tcttcggcaa tacggcgatt tggctgaatg acggggttgc ggggggaatg
     3661 ctcgaaaaga tgacctcctc tccggaaacg ctgcttttta aattctttaa ttacctcccc
     3721 ctgcccgaat tgacgagcat cgtcagcctg ctggtcattt ctctgttttt tgtaacttct
     3781 gccgattccg ggatttatgt cctgaacaat attacctctc gggacaaagg cttgagcgcg
     3841 ccacggtggc aggcggttat gtggggcgtg ctgatgtctg ccgttgccgt tttgctgatg
     3901 cgctcgggcg gactcggcaa cctgcagtct atgaccctga ttgtttccct gccgtttgcc
     3961 ctgctgatgc tgataatgtg tttcagcctg tggaaaggct tgagtgcgga taagaaatat
     4021 tttgagaccc gggttaaccc taccagtgta ttttggacgg gcggcaagtg gaaagaacgg
     4081 ctggtgcaga taatgagcca gacgcaggag caggatattt taaaattcct caaacatacc
     4141 gcatcgcccg ctatgcacga gttgcaacgg gagctttcgg aagaatacgg cttgagcgtc
     4201 cgggtcgata agatgtttca tcaggacgag cccgcaatcg agttcgtcat tcggaaagag
     4261 acgatgcgcg attttatgta cgggattaag tctgtcgggc aggatgtatc cgaccagttg
     4321 attaacgacg gcaagctgcc gcatatccgg catcagacaa cttacaaacc ctacgcttat
     4381 tttttcgacg ggcgcgtcgg gtacgatgtg cagtatatga acaaggacga gctgattgcc
     4441 gacattttga aaaactacga acgttatttg atgttgttgg atgatgtcgg tcaggaactg
     4501 atggcgcacg agcaggtgga attggcagag taagtgcctt atctctggcg tttgccaaaa
     4561 aaaatgcggt gaatcagatt caccgcattt tttttgccgg acggggcttc ggacggcacg
     4621 gcgttcattt gtttccgtca aactgctcct tcaacacccg tttcaatacc ttgcccgtag
     4681 cgttgcgcgg cagcccgtct ttaaagtgga tttgtttggg gattttgaaa tttgccagca
     4741 cggtacgcag gtggcggcgg atttcgttct cgcccaaatc cataccttcc ttcaattgga
     4801 cgaaggcgac gatttcctcg tcggcataac ggtctttcac gccgatgacg gcggcggctt
     4861 cgacggcatc gagtttgtag atttcttctt cgatttcgcg cggatagacg ttttgacctt
     4921 tggaaataat caaatctttt ttgcggtcga cgataaagat aaagccgtct tcgtctatgg
     4981 taacgaaatc gcccgttttc aaccagccgt tgacgatggt ttcatcggtg gcggcaggca
     5041 tattgaggta gccccgcatc accgaaccgc ccctgacgat cagttcgccc acttcgccgc
     5101 gcggcacttc gaccaattct tcatcgacgg ctttggcttc caaaccgggc agggggatgc
     5161 cgacgctgcg ggctttttgc ctctcgggcg tattgacggc gacgacgggc gaggcttcgc
     5221 tcagtccgta gccttccagc aatttggcgc gggggaactt ggctttgaaa tcgaggatgg
     5281 tttgttccgc caaaggtgct ccgccgctga taaacaggcg gatgcggttg aaccatctga
     5341 aataccaagg gattttcgtc ttgctcatcg cggtgtaaat cgcgggcacg cccaaaaaca
     5401 cggtcgcgcg tttgagcagt gcctgtttca aaacgttgga aaaggggaaa acggatttga
     5461 ccaaaataat cgaacacgcc atataaatcg gcagcagcac catagccgtc agcgtgaagc
     5521 tgtggaacat cggcaggaaa acgataaagc ggtcgcgctt ggagatttta aagatgcgtt
     5581 cgatgccgtt caggttggcg aacaggttgg cgtagctgat tagcgcacct ttgggatgcc
     5641 ccgtcgtgcc ggaggtgtag atgatgtgtg ccaaatcatt tatccggggt tggcggccca
     5701 agtcgggttt ttcggggaag cggcgcacgt cttcaaaaaa ggcatcgcct tccgccattt
     5761 cgccgtccgg acggctttgg cccgtccaaa tgattttttc gacgggcgtt tgcgccttca
     5821 agcccgccaa ttcttttgac aggccggccg aggcgaacag gaagcgcgcc ttgcagtcgt
     5881 tcaggatata cgcgtattcg ctgtttttca aaaatgtgtt catcggtacg gcaaccgcgc
     5941 cgatggcgga tacggcgaaa taggcggtaa taaattccgt ggaattggaa accgccagcg
     6001 cgaccgtgtc gccgaacttc acgccgatat tttgcagata cgccgcaacg gcttcggctt
     6061 cctgcttgag cgcgcggtag gcggtttttt ccttgccgtc gaacactgcc gtgccgttgc
     6121 cgtttttgcg gcaggcggcg gtcagcattt cgtagaaatt ggcataagtc cggttcatga
     6181 aacttccttt atggttgcgg acggcgaacg ccctcatttg aaaacaatat gccg
//
LOCUS       DAWFLT010000105         6183 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWFLT010000105 DAWFLT010000000
VERSION     DAWFLT010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.105"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            24..407
                     /locus_tag="WGR74_001942"
     CDS             24..407
                     /locus_tag="WGR74_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ0906293.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKLA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            522..995
                     /locus_tag="WGR74_001943"
     CDS             522..995
                     /locus_tag="WGR74_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ0906294.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMRIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVEGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMPAMNHGHHHGEAHQH"
     gene            1121..2224
                     /gene="mnmA"
                     /locus_tag="WGR74_001944"
     CDS             1121..2224
                     /gene="mnmA"
                     /locus_tag="WGR74_001944"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ0906295.1"
                     /translation="MNTTANTTNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDDETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            2295..3965
                     /locus_tag="WGR74_001945"
     CDS             2295..3965
                     /locus_tag="WGR74_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ0906296.1"
                     /translation="MKKIWLDSYEKGVSAEIDITQYNSVSDVFRQSVEKFADQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGQLKKQRVNVCIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWKRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            4077..5711
                     /locus_tag="WGR74_001946"
     CDS             4077..5711
                     /locus_tag="WGR74_001946"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ0906297.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSGHIRERHRHRYEVNNNYVPQLEKAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
     gene            5726..5928
                     /locus_tag="WGR74_001947"
                     /pseudo
     CDS             5726..5928
                     /locus_tag="WGR74_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246312.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5939..>6183
                     /locus_tag="WGR74_001948"
     CDS             5939..>6183
                     /locus_tag="WGR74_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0906298.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDPVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDSELEHSL"
BASE COUNT         1617 a         1683 c         1548 g         1335 t
ORIGIN      
        1 gagaacattt tgaaaggata ccgatggaac cttcttctta cgcggcagaa aaaaaaggca
       61 aaggcggcat caggcgcgtc attaacgcat tcggctattc gatagacggc atcgccgccg
      121 cctaccgtta cgaagcggca ttccgtcagg ttttgtggct gaacgtactg ctggtgtgcg
      181 cggcattttt ttgggtttcc gaaaccgccg tccgcctgcc gttgattatc gcgtcttttg
      241 tgtcggtcat tgtcgaactg ttcaacaccg ccgtcgaagc tgccgtcgat catacttcga
      301 ccgaaaaaca cgagctggcc aaactcgcca aagacgcagg ttctgccgca caattgttcg
      361 cgatgctgat gttggcggcg gtttggctgt ctgccctgtt cgggtaaaac gcttgcagca
      421 ggatttgtaa tcctttagga ttagtatgtg gtatttatta ttcacttaat ccgctataag
      481 attaagcaca ttccccgtct gatattataa ggagcagaaa gatgaaaaaa ttattggcgg
      541 ccgtgatgat ggcaggtttg gcaggcgcgg tttccgccgc cggagtccac gttgaggacg
      601 gctgggcgcg caccaccgtc gaaggtatga gaataggcgg cgcgttcatg aaaatccaca
      661 acgacgaagc caaacaagac tttttgctcg gcggaagcag ccccgttgcc gaccgtgtcg
      721 aagtgcatac ccacatcaac gacaacggcg tgatgcggat gcgcgaagtc gaaggcggtg
      781 tgcctttgga ggcgaaatcc gttaccgaac tcaagcccgg cagctatcac gtgatgttta
      841 tgggtttgaa aaaacaatta aaagagggcg acaagattcc cgttaccctg aaatttaaaa
      901 acgccaaagc gcaaaccgtc caactggaag tcaaaatcgc gccgatgccg gcaatgaacc
      961 acggtcatca ccacggcgaa gcgcatcagc actaatctgc tgaaaatgtt tgaaatgccg
     1021 tctgaaaaag cccgggcttt cagacggcat ttttatgccc gcctttaaaa tgtgttaaaa
     1081 tccgccttta aaaaccgccg tttccaacca tattctacgc atgaatacga cagcaaacac
     1141 caccaatatc atcgtcgggc tctccggcgg tgtcgattct tccgtaaccg ccgccctgct
     1201 caagcagcag ggttatcaag tgcgcggtgt gttcatgcag aactgggaag acgacgacaa
     1261 cgacgaatat tgcagcatca aacaggattc gttcgatgcc atcgccgttg ccgatattat
     1321 cggcatcgat atcgacatcg ttaatttcgc cgcgcaatat aaagacaaag tctttgctta
     1381 ttttcttcag gaatacagtg cggggcgcac gccgaatccg gatgtgttgt gcaacgccga
     1441 aatcaaattc aaatgctttt tggactacgc cgtagggcag ggcgcggata ccatcgccac
     1501 cggacactat gcgcgcaaag aagtccgcaa cggcgtgcat tacctgctca aaggtttgga
     1561 tcgaaacaaa gaccaaagct atttcctcta ccgcctcaag cctttccaac tcgagcgcgc
     1621 gatttttccg ttgggcggtt tggaaaaacc cgaagtgcgc cgccttgccg ccgaatttaa
     1681 tttgccgact gccgctaaaa aagacagcac gggcatctgt ttcatcggcg aacgcccgtt
     1741 tcgcgagttt ctgcagaaat acctgccgac cgacaatggt aaaatggtta cgcccgaagg
     1801 gaaaaccgtc ggcgaacacg tcgggctgat gttctacacg ctcggacagc gcaaaggttt
     1861 gggcattggc ggcgcgggcg aaccgtggtt tgttgcggct aaagatttga cgaaaaacga
     1921 actgattgtc gtgcaaggac acgaccatcc gctgctttat acccgcagcc ttgtgatgaa
     1981 cgatttgagt ttcacgctgc ccgaacgccc gaaggcagga cgctatacct gcaaaacgcg
     2041 ttaccgtatg gcggacgcgc cttgtgagtt gcgctatttg gatgatgaaa ccgccgagct
     2101 ggtgtttgac gaaccgcaat gggcggttac gccgggtcag tccgccgtgc tgtacgacgg
     2161 cgatatctgt ttgggcggcg gtatcatcca aacgaccgac aaacccgtca tcatcacacg
     2221 ataaaggtaa tgccgtctga aacggttttc agacggcatt gttccgctca attccacttt
     2281 aaagaccgat acctatgaaa aaaatttggt tagacagcta cgagaagggc gtcagtgccg
     2341 aaatcgacat cacgcaatac aattccgtca gcgacgtatt ccgccaaagc gtggaaaaat
     2401 ttgccgacca acctgctttt caaaacatgg gtaagacgct gacttatgcc gaaaccggca
     2461 aactggcaac tgatttcgcc tcttatctgc aaaacgtcct caagctgccg cgcggcgaac
     2521 gtgttgccat tatgctgccc aacgtattgc aatacccgat tgcccttttc ggtattttgc
     2581 aggcaggttt ggtggcggtg aacaccaatc cgctctatac accgcgcgag ttggagcatc
     2641 agttgaaaga cagcggtgcg accgccatca tcgttttgga aaatttcgcc aacacgctgg
     2701 agctggtgct gccgcgcacg cagatcaaac acgtcatcgt cgcctccgtc ggcgaaatgt
     2761 tcgggctgct taaaggttcg ctgatcaatt tcatcatccg gaaaatcaag aaaatggtgc
     2821 ccgaataccg tattcgggaa accgtttcct ttcagacggc attgaaagaa ggggcgaagc
     2881 acgttttcca acctgtcgca ttaaaccgcg aagataccgc gctgttgcaa tacacgggcg
     2941 gcacgacagg tgttgccaaa ggggcggtgc tgagtcacgg caacatctgc gccaatatgc
     3001 ttcaggcaaa agaatggatt aaaaaccagt tgcgcgaggg caaagaaacc gttatcgccg
     3061 ccttgccgct gtaccatatt ttcgccttaa ccgtgaatct gatgattttt gccaatgccg
     3121 gctcgaaaat catcctgatt accaacccgc gcgatatgaa aggctttatc ggacaactga
     3181 aaaaacagcg ggttaatgta tgtatcggcg tgaacacact gtttaacgca atggttaacc
     3241 gtcctgattt tgccgaagtc gatttttcag gattgcggtt gactttgggc ggcggtatgg
     3301 cgacccaaaa agccgttgcc gaaaaatgga aaaaaatcac cggcacgccc atcgtcgaag
     3361 cctacggtct gaccgaagcc agccccggcg tgtgctgcaa ccccttaaac atcgaatcat
     3421 acagcggcag catcggtttg cccgtctcgt ccaccgaagt cgaactgcgc gacgcaaacg
     3481 gcaaagaagt ccccgttggg cagccgggcg aattgtgggt aaaaggccct caagtgatgc
     3541 aaggctactg gaaacgcccc gaagaaaccg ccaaagccat agacgcgcgc ggctttttgg
     3601 aaaccggcga tattgccgtg atggacgaaa aaggctggtt gaagctggtc gatcgtaaga
     3661 aagacctcgt cgttgtttcc ggattcaatg tttatccgaa cgaaatcgag gaagtcatcg
     3721 cgcatcacgg caaagtcatg gaagtcgcct gtatcggcgt tcccgacgaa aaaaccggag
     3781 aagccctaaa agtattcgtc gtcaaaaaag acccgtcatt gaccaaagaa gaacttatcg
     3841 ccttttgccg caccgaatta accgcatata aagtaccgaa aaatatcgaa ttccgcgacg
     3901 agttgcccaa gtccaacgtc ggcaaaattc tgcgtcgcga gttgcgccaa agtactggga
     3961 aataaagaaa agatgccgtc tgaaaacagc cgtccaccgt tcagacggca tccgcctgtt
     4021 tgccagaacc gcgcgcttca cgttaaaatc acgcattcca atacgggtat ttcattatga
     4081 ccaaattcat tttcgtcacc ggcggcgttg tctcctcact gggtaaaggt atcgccgccg
     4141 cttctattgc cgccatcctc gaatcgcgcg gcttgaacgt taccatgctc aagctcgatc
     4201 cttatatcaa cgtcgatccc ggcacgatga gcccgttcca acacggcgaa gtgttcgtaa
     4261 ccgacgacgg cgcggaaacc gacctcgact tgggacacta cgaacggttt atcgattcca
     4321 cgatgacccg ccgcaacagc ttcagcacgg ggcaggtgta cgaaaacgtc atcgccaaag
     4381 aacgacgggg cgactacctc ggcggcacgg ttcaagtcat cccgcacatt accgacgaaa
     4441 tcaaacgccg catccacgaa ggcgcggcag gttacgatgt ggcgattgtc gaaatcggcg
     4501 gcacggtcgg cgacatcgaa tcgctgccgt ttttggaagc catccgccag atgcgaagcc
     4561 agttgggacg caacaacacc ctgttcgccc acttgagcta cgtcccctac atcgccgctg
     4621 caggcgaaat caaaaccaag ccgacccagc acaccgttaa agaaatgttg agcatcggtt
     4681 tgcaacccga catcctgatt tgccgtatgg acaggacaat gcctgcggac gaacgccgca
     4741 aaatcgcctt gttctgcaac gtggaagaac gcgcgattgt cggcagctac gatgtggaca
     4801 gcatctacga atgccccgaa atgctgcacg accaaggcat cgacaacatc attaccgagc
     4861 agttgcagct taatgtgcaa caggcggatt tgaccgcgtg gaaaaaaatc gtccacgcca
     4921 tccaaaaccc gaaacacacc gtcaaaatcg ccatggtcgg caaatacgtc gatttgaccg
     4981 agtcctacaa atcattgatc gaagccttga aacacgcagg cattcacacc gaaaccgacg
     5041 tacaaattac ctttgtcgat agcgaaagca tcgagaaaaa caacggcgac gtttctatgc
     5101 tcaaagatat ggatgccatc ctcgttcccg gcggtttcgg ttcgcgcggc gtggaaggca
     5161 aaatcgccgc tgtgcgctat gcccgtgaaa acaacgtgcc atacttgggt atctgcctcg
     5221 gtatgcagat tgcgctgatt gaatacgccc gcgacgtggc aggtttgaaa ggtgcgaatt
     5281 ccactgagtt cgacctcaaa tgcgccgccc ccgtcgttgc cctgattgac gaatggcaaa
     5341 ctgccgacgg cagcgtcgaa acccgcgatg aatccgccga tttgggcggc acgatgcgtt
     5401 tgggcgcgca agaagtcgaa ttgaaagcag gcagcctcgc cgccaaaatc tacggcagcg
     5461 gacatatccg cgaacgccac cgccaccgct acgaagtcaa caacaactat gttcctcagt
     5521 tggaaaaagc aggcttggtt atcggcggcg tatccgccgg acgcgaacgc ttggtcgaaa
     5581 ccatcgagct gccgaaccac ccttggttct tcgcctgcca gttccatccc gaattcactt
     5641 ccaacccgcg caaagggcat cccttgttca ccgcgtttgt caaagctgcg ttgaacaata
     5701 aaaaagcctg ataaagcgtt acttgatgat caaaatgccg tctgaaagcc tgaatgaaga
     5761 ctttcagacg gcattttcgc aaatcgggga ttataaaagc catcaattta atttttatgg
     5821 ttttatctaa tatttaccaa ctgaatattg ttaagcgggg cgtgaaactc aagttgagac
     5881 ctttgcaaaa ttccccaaaa tcccctaaat tcccaccaag acatttagga gatgttccat
     5941 gagcaccttc ttccaacaaa ccgcccaagc catgattgcc aaacacatcg accgtttccc
     6001 gctattgaag ttggatccag tgattgattg gcagccgatc gaacagtacc tgaatcgtca
     6061 aagaacccgt taccttagag accaccgcgg ccgtcccgcc tatcccctgt tgtccatgtt
     6121 caaagccgtc ctgctcggac aatggcacag cctctccgat tccgaactcg aacacagcct
     6181 cat
//
LOCUS       DAWFLT010000106         5941 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWFLT010000106 DAWFLT010000000
VERSION     DAWFLT010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.106"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(228..551)
                     /locus_tag="WGR74_001949"
     CDS             complement(228..551)
                     /locus_tag="WGR74_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ0906299.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(632..1288)
                     /locus_tag="WGR74_001950"
     CDS             complement(632..1288)
                     /locus_tag="WGR74_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ0906300.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     ISDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDADPFGGFQF
                     "
     gene            complement(1390..1893)
                     /locus_tag="WGR74_001951"
     CDS             complement(1390..1893)
                     /locus_tag="WGR74_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ0906301.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAV"
     gene            2077..2850
                     /locus_tag="WGR74_001952"
     CDS             2077..2850
                     /locus_tag="WGR74_001952"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ0906302.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2857..3207
                     /gene="secG"
                     /locus_tag="WGR74_001953"
     CDS             2857..3207
                     /gene="secG"
                     /locus_tag="WGR74_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ0906303.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            3221..3306
                     /locus_tag="WGR74_001954"
     tRNA            3221..3306
                     /locus_tag="WGR74_001954"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3255..3257,aa:Leu,seq:gag)
     gene            4107..4475
                     /locus_tag="WGR74_001955"
     CDS             4107..4475
                     /locus_tag="WGR74_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ0906304.1"
                     /translation="MRIFKNQWIVKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
     gene            4472..4783
                     /locus_tag="WGR74_001956"
     CDS             4472..4783
                     /locus_tag="WGR74_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ0906305.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(5120..5935)
                     /locus_tag="WGR74_001957"
     CDS             complement(5120..5935)
                     /locus_tag="WGR74_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0906306.1"
                     /translation="MVCLSAVISILGGCAVVADVYGHDSATMNAAAAEDYMKTVELNK
                     SAGNVDTTSRTARRVQAVFRRMLPYADAANNTGHRFDWKMTVFKNDELNAWAMPGGKM
                     AFYTGIVDKLKLTDDEIAAIMGHEMTHALHEHGKNKVGQQILTNTAAQIGTQIILDKK
                     PDTNPELVGLGMDILGTYGITLPYSRSLEEEADEGGMMLMAQAGYHPAAAVRVWEKMN
                     QENDQNGFIYAITSTHPTNNARIENLKRLLPTVMPVYEQSVRNKGRVNKKRRR"
BASE COUNT         1627 a         1734 c         1329 g         1251 t
ORIGIN      
        1 cagcctgcac attgaaaatg ccgcccaagc aaaccttcag tttgccagcc tcgtcctagc
       61 cctctcccac ggggagaggg gattggggtg caggctgcct ttaaggttca ggcaaatttt
      121 aacttcgttg ataccgcgct ttagcttcgc agaagctgca ctttcagacg accttttgga
      181 acaccacagg tacacgcatt taaggaatgc cgtctgaaat gcctgcctca ataacgcatc
      241 atgttgccgt caatctcggc cgcccatgca tcgatgccgc cctgaaggtt gtacaggttt
      301 tcaaaacccg cgtccgccaa atacatcgcc gtatgcagac tgcggatacc gtgatggcaa
      361 tacaccacaa gcggcacatc atccggcagc tcgttctgcc gcagcggaat cagattcatc
      421 gggatatgca gcgcatttgg cagcgaacaa accgccgttt cttcatccgt acgcacgtcc
      481 aacaaacaaa acatccgccc ttcgtccatc cacgccttca attccgccga cccaagttgc
      541 acaatatcca tcgcaccccc caaaaaaaaa accaagcaaa atgccgtctg aagccccaaa
      601 cccgctttca gacggcatcg caatccgaac atcaaaactg aaaaccacca aacggatcgg
      661 catccttatc atccaaatgc gccaccaagg tatcgaacag caccttctct tcaaacacat
      721 cgcccctgcg cgtaatcaaa agcgcgcgtt gaacaggctt gcgtcctacg ataaccacca
      781 tgcgtccgcc atctttcaac tgttctttca acacttcagg cacaaggttt accgcaccgc
      841 cgacataaac cgcatcaaac ggcgcacctg cggaaagttc ggtcaacccg ttgttttgca
      901 cataatcgat attgtccaaa cccaagccgt ccaacaccgc tttggcgcgg ttttgctgtt
      961 ccgcatcgat atcgtccgaa attaccctgc ccgccaattt tgccaacagc gcggtcgcat
     1021 agcccgaacc cgtgccgatt tccaaaaccg tatcgttttt cgtcagcttc aagccctgcg
     1081 ccagccgcgc cacgactttc ggctccagca tcttatgacc gttggcaagc ggcagcgcca
     1141 tatccgcata cgccaaaccc tgcaagtcct catcgacaaa aagctcgcgc ggaatctccg
     1201 ccaaagcgtc caaaacgtca aaatccaata catcccaagg acggatttgc tgttcgacca
     1261 tattgaaccg cgctttttca aaatccattt agtgctccgt caaataattg ttccaacaat
     1321 agcaggcatt ataaaaccac actcccgccg cgccaacttc ggcacgcgcc gcccgcccct
     1381 ttccctcact caaaccgcct gacgcgaagc ctcaaaccgc ttctccaaaa tcttcgccag
     1441 ctcgcccaaa tacaaagcat ctgtctcatc aaactgcgcc aaatgttcgc tgtccgcgtc
     1501 caacacgccg atacagcggc cgtctgaaaa cagcggcacg acaatctccg aacgcgacaa
     1561 agacgaacag gcaatatggt cgggatgcgc gtccacatcc ccgacgacca ccgtcccgcc
     1621 cttcgcccaa gcctgaccgc acaccccgcg accgaacgga atccgcgtac acgccaaagg
     1681 cccctgaaac ggtgccaaaa ccaattcgtc cgaacgcgta tcgaccaaat aaaaacccac
     1741 ccaaaaccaa ccgaacgcct ccttcaaaac cgccgccgtg ttcgccaaat tcgccaccca
     1801 atccgtctcg tccgccacca cagactcaat ctgcggcaac acctcccgat aaagcgcggc
     1861 cttgtccgaa gccgaaaaat gaagcgcgtg catcacatct cctatagttg catacatatc
     1921 aggcggccat tataaaacaa cctgcccgaa acaacattcc aaaccgcccc cgcagccgcc
     1981 cgccccaagt tgcgaacccg ccgcatatcc actaaacttc acattgcaac gcgccacacg
     2041 cggcagacag aaaaaacacg acacggagca aaaaagatgt atcaccaaat cggaatgtgg
     2101 gatcaaaaat gggtcatcgg caactggaaa atgaacggcc ggctccagaa caacaattcg
     2161 ctcatgcacc gcttccgcat cctccccacc gccgaacgcg tcctcatcgg actcgccgcc
     2221 ccgaccgttt acctgctgca actgcacaac gccatgcaaa tcgtcttaaa caaccgcatc
     2281 ctcacctgcg cccaagacgt gagccgcttc cccgacaacg gcgcgtacac cggcgaagtg
     2341 tccgccgaaa tgctcgccga caccggcaca gacatcgtcc tcatcggaca ctccgaacgc
     2401 agcctttatt tcggcgaaaa aaacgaaatc caacgccgca aaatggaaaa cgtcctcaac
     2461 gtcggactca tcccgttatt gtgcgtcggc gaaagcctcg aagagcgcga agccggcaaa
     2521 gaacacgaag tcatcgccca tcagctttcc atcctgcaag ggctggatac caaaaacatc
     2581 gccgtcgcct acgaacccgt ctgggcgatc ggcaccggca aagtcgccac cgtcgaacag
     2641 attgccgata tgcacgcatt catctacaaa gaaatcttgt ctttgtgcgg aagcgatgtt
     2701 aaaatccgcg tcctttacgg cggaagtgtg aaagcggaca acgcggccga catcttcgca
     2761 gtaccttatg tggacggcgc actcgtcggc ggcgcgtcat tgtcgtacga ctcctttacc
     2821 gccatcatca gtgccgcaca aaatgcgtag aaaaatatgg aagccttcaa aaccctaatt
     2881 tggattgtta atataatttc cgctttggcc gtcatcgtgt tagtattgct ccaacacggc
     2941 aaaggcgcgg atgccggcgc gactttcgga tcgggaagcg gcagcgcgca aggcgtattc
     3001 ggctctgccg gcaacgctaa cttcctcagc cgctcgaccg ccgttgcagc aacatttttc
     3061 tttgcaacct gcatggctat ggtgtatatt cacacccaca cgacaaaaca cggtttggac
     3121 ttcagcaacg tacaacaaac tcagcaagca cccaaacccg taagcaatac cgaaccttct
     3181 gcccctgttc ctcagcagca gaaataacag tttttcaaat gccgacatgg tgaaattggt
     3241 agacacgcta tcttgagggg gtagtggccg taggctgtgc gagttcaaat ctcgctgtcg
     3301 gcaccaacac acaaaacgct tagaaattct aagcgttttt ttcgtttaat cctgcctgat
     3361 gccatcaacc aatcccaatc aatacaaggt tttcaggctg attgttatcc tgccgccccc
     3421 ccttcctgac cgtacaatcc cgtccaatcc gccctaattg aactaacctg attttttacg
     3481 gcatccgaaa ataccgcgaa aaatacgtga aaataaagct gaacgaccac caaatcagga
     3541 atgccaagcg ggaaatagct tgcggggaat actgacgcac cgtagggaat gagggggaaa
     3601 ccgtccaaga tgcagagcgg ttttttttgg gtttttggaa aaaacctata ctatagtgga
     3661 ttaaatttaa actggtacgg cgttgcctcg ccttgccgta ccggtttaaa tttaattcac
     3721 tataaaataa gaaaaagata aaaaattggt aacaaatgcg gtaacaaatg gtaacgaatc
     3781 ggtaacaact tttggggttt tccggttttt caccgtcttg gcagtgggag cgtagcggaa
     3841 tgaaaagcca aaacgcacgg aaccgcgcct attttgagca ggaatggcgg ttaaaccact
     3901 tggttatata tgggggcata ggaagacagc gaaacgcgct aaaaacgcaa tttgagccat
     3961 taaaagcgat tgattaaaaa ataatcaggt tagccgccag tgtttcaggc ggcataaacg
     4021 gagaaattgc ggggcataaa aaaggcagct tgccgtgttg tctgtctctg gtataattcc
     4081 aagtatcact aatcaacggc tacacaatgc ggatattcaa aaaccaatgg atagtgaaat
     4141 ttgccaagaa gcacaaaatc aacgattccg agctgctgga agcggtagag cgggcggata
     4201 acgggctgat agacgcagat ttaggcggcg gtgtgattaa gcagcgcata gcaaggcaag
     4261 gacaaggcag aagcggcggc tatcgcagcc tgatattgtt caaacaggca gatagggcat
     4321 ttttcgttta cgcctttgcc aagaacgaca gggaaaacat ttcggataaa gaactggacg
     4381 tttaccgaaa agccgccgca tattatctga aatacacgcg ggcagagctg gcggctttga
     4441 aagaagacgg cattatcacg gagatagaat catgaaatac aaaagcgagg cattagccgc
     4501 cattcatgaa atgatggaag gggcttacaa catcggcgca atcgacaaaa agactatgcg
     4561 cggctttgac aagtcatgcc tgaccgaaat caaaccgttg agcggcggag acatcaaggc
     4621 aatcagggag aaggaggcac tatcgcaagc cgctttcgcc atctatctca acgtgggaaa
     4681 aaatcacgtt tcggcttggg agcggggcgt taaaaagccg agcggcgcgg cgttgaagct
     4741 gctgaccatc gtcaaaaaca agggtatcga agccattgcg tagccgactt ggcaaacggc
     4801 aaaatcagca agttcacaat agacgcgctg ctgaatatgc ctgccaaaac aggcaagacc
     4861 gccgaactga atatcagggc gtagccgcat aaatgccccg accgcatcaa accaagccga
     4921 aacggcggcg gtgcagacgg catagcccga cagcaaggca cggggatttt tttgcgcccg
     4981 ttgcaggggg attggattta agcggcgcgg acttgaaggc aaaacgggcg gggcgcagaa
     5041 ctgtttaaat gccgtctgaa tctcaaacgg attcagacgg cattttggaa caacggctca
     5101 aattctcgat ccccttccct taacgccgac gttttttatt aacgcgcccc ttatttctga
     5161 cactttgctc ataaaccggc ataacggtcg gcaacaaccg ttttagattt tctatacggg
     5221 cattgtttgt cggatgagta gaggtaatag cataaataaa gccgttttgg tcgttttcct
     5281 gattcatttt ttcccaaacc ctgacagcgg ccgccggatg atagcctgcc tgcgccatca
     5341 acatcattcc cccctcatcg gcttcttctt ccaagctgcg gctataaggc aaggtaatac
     5401 cgtacgttcc caaaatatcc atacccaatc cgaccaattc cggattagta tccggttttt
     5461 tgtctaatat aatctgcgtg cctatctgcg ccgccgtatt ggtcaagatt tgctgcccga
     5521 ccttattttt accgtgttca tgcagggcgt gcgtcatttc atgtcccata atggcggcaa
     5581 tttcgtcatc ggtcagcttg agtttgtcga ctatccccgt ataaaacgcc attttcccgc
     5641 cgggcatcgc ccacgcgttc agctcatcgt ttttgaaaac cgtcattttc cagtcaaacc
     5701 tatggccggt attatttgcc gcatcggcat aaggcaacat acgtcgaaat actgcctgca
     5761 ccctacgggc tgttctggat gtggtatcga cattgccggc agacttgttt aactcaaccg
     5821 ttttcatata atcttcggca gccgcagcgt tcattgtggc ggaatcatga ccgtaaacat
     5881 cagcaacgac cgcacaacct cccaatatcg aaatgactgc cgacaggcag actatccgtt
     5941 t
//
LOCUS       DAWFLT010000107         5573 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWFLT010000107 DAWFLT010000000
VERSION     DAWFLT010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.107"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(380..3121)
                     /gene="tbpA"
                     /locus_tag="WGR74_001958"
     CDS             complement(380..3121)
                     /gene="tbpA"
                     /locus_tag="WGR74_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ0906307.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDTNRYANFIVEEECENRNYETCKANP
                     KKEVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHT
                     QQTFDTRDMTVPAFLTKAVFDENRKYGPIRGNGKYAGNHKYGGLFTNGENGALVGAEY
                     GTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGS
                     DKNCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGSN
                     LRHQDYYFQNAKRAYSLKTPPKNGNQNSPNGDKSKPYWVSIGGGNVVTEQICRLGNNT
                     YTGCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDSSVSTGTHRTLSW
                     NAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGIVF
                     KGDFGNLEASWFNNAYRDLIVRGYEAQIQNGKETAKGDPAYLNAQSARITGINVLGKI
                     DWNGVWDKLPEGWYSTFAYNRVHVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYDQP
                     EGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTIKK
                     HFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF"
     gene            complement(3207..5372)
                     /locus_tag="WGR74_001959"
     CDS             complement(3207..5372)
                     /locus_tag="WGR74_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0906308.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPQAQKDQGGYGFAMRLKRRNRHPTAMPKENEVKLKNDDWEATGLPTEPKKL
                     PLKQESVISNVQTDNGDNNIYISPYLTQSSHNSHNGSINGGANQPKNEATGYNNFKYV
                     YSGWFYKHAASERKFNEGKFKSGDDGYIFYHGKDPSRQLPASGKVTYKGVWHFVTDTK
                     LGQKFNDIIQPSKKQGDSYSGFSGDEGEEYSNKNESTLKDGHEGYGFTSNLEVDFGNK
                     KLTGKLIRNNASQSNTNNDKPTTQYYSLEAQVTGNRFNGKAIATDKPQENETKQHPFV
                     SDSSSLSGGFFGPQGEELGFRFLSNDQKVAVVGSAKTQDKPRNGAVASGGTGAAASDG
                     AAGTSSKNGKLTTVLDAVELTHGGTAIKNLDNFSNAAQLVVDGIMIPLLPEASESGKN
                     QANQGTNGGTAFTYKTTYTLESDKKDTKAQTGAGGAQAASGAAGVNGGQAGTKTYEVE
                     VCCSNLNYLKYGMLTRKNSKSAMQAGENGSQADAKTEQVEQSMFLQGERTDEKEIPKE
                     QQDIVYRGSWYGHIANNTSTSWSGNASNATSGNRADFTVNFGDKKITGTLTANDRTQP
                     TFTITANIKDNGFEGTAKTAELGFDLDQSNTTGTPKAYITNAKVQGGFYGPKAEELGG
                     WFAYSGDKQTENTTVASGNGNSASSATVVFGAKRQQPVQ"
BASE COUNT         1160 a         1418 c         1386 g         1609 t
ORIGIN      
        1 cccatcattc ccgcgcagga gggaatccga atacatccga acagaaacct gcatcccgtc
       61 attcccgcga aagcgggaat ctagaacgta aaatctaaag aaaccgtttt atccgataag
      121 tttccgcacc gacaggtcta gattcccact ttcgcgggaa tgacgggatt tgaggtttct
      181 gtttttgatt ttctgttttc gtaggaatga cggtttagag ttacccgaaa cctcaaaaaa
      241 aaccgaaacc gaacggactg gattcccgcc tgcgtgggaa tgacgggttt caagattacg
      301 gtattgtcgg ggatgatggg aaacggtggg aattgtgtaa aaaatgccgt ctgaagcctt
      361 tcagacggca tttggacgtt taaaacttca tttccaagct aaatgtgtag tttcggccgg
      421 gggcggcata tcggttgtaa acgccgacat ttttgtgttg gttgactgcg ccgccggcag
      481 tttgccgcac attttcccaa gtaacatagc ggtagttgag gaggttgtac acgcccgcac
      541 ggagggtgaa gtgtttttta atcgtgtaat aaccggacac atccacaata taccaagggc
      601 gggtacggcg cgcggtggct tttgtattgc ggctgttgcc gttgagcaaa gcacggctgc
      661 ccaacaactc tgtgatttcc ttggctttgg aataagtcag cataccgttc acaccccatt
      721 tgccttccgg ttggtcatag cccaagccga cgacatagcg cgagggttgg atggcatcaa
      781 acaggtgtga ttgaatatcg gtgcggtctg cgcgtttttt gatgtcgcgg acatggacac
      841 gattataggc aaatgtagaa taccaacctt cgggcaattt atcccatacg ccgttccaat
      901 cgattttgcc caaaacattg atgccggtaa tccgcgcgct ttgggcattg aggtaagccg
      961 ggtcgccttt ggctgtttct ttgccgtttt gaatttgcgc ttcataaccc cggacaatca
     1021 aatcgcggta ggcattgttg aaccaacttg cctccaagtt gccgaaatcg cctttaaaca
     1081 cgatgccggc ttctttgttg aacgattttt ccggatcgat tttgaccgcc ttgctttgaa
     1141 cacccgcccg ccagccgtac atttccgcaa acgagggcag gcggaagccg gttgaagtgc
     1201 ggtaagtcaa atccagccag tcggcaggtt tgaggacgat gcctgcgttc caggacaggg
     1261 tgcggtgcgt gccggtggaa acgctgctgt cgtccgaatg cgtgctgcgg tagtcgtagc
     1321 gcaaccccgc gccgacatcc gcccacctgc ccaaacggac attgtcccga actgccgcgt
     1381 aatagctttt gccgttgatg ctgcgcggcg tgcagcccgt ataagtattg ttgcccaagc
     1441 ggcagatttg ctccgtaacc acatttcccc cgcctatgct gacccaatag ggtttgctct
     1501 tgtcgccgtt ggggctgttt tgattgccgt ttttaggggg cgttttcaac gaataggcgc
     1561 gtttggcgtt ttgaaaataa taatcctgat ggcggagatt agagccaaag cggtcaaacc
     1621 cgagattcac gctcaggttg tggcggattt tggcggtatc gaaggatttt ttgaatgccg
     1681 cctgcaagag cctgtggctt tccccgtaaa tcacgcggtc ggatttgtaa taggaaaacg
     1741 gcttgtcggc actcggacgg caatttttgt ccgaaccgtc ggcagaacag tgcgtctgct
     1801 gaaaatgatt gtccaaaccg ataccctgcc ggtcataaga gaggcgggca taatccgccc
     1861 aagtgtcttt atcggcattg gtatagacat attccaaacc gtagcggctt ttggtgtgcg
     1921 tctcgtcgta aaacacgcct gtaccgtatt ccgcgcccac cagcgcaccg ttttcgccgt
     1981 tggtaaacag tccgccgtat ttgtggttgc ccgcatattt gccgttgcct cttatcggtc
     2041 cgtattttct attttcatca aaaaccgcct tggtcaggaa tgccggaacc gtcatatcgc
     2101 gcgtgtcgaa agtttgttgc gtgtgttcga gtatgccgcc gatgtagtgc cgtttgtttt
     2161 caaaacgaaa acccgggcgg aacaaccacg atcggctttc gtatgaaagc ggatcggcga
     2221 ggaagcggtt gggacccgtg tagtctcggg tggaaaccgt ttgccgttcg tctttgccga
     2281 caacctcttt tttcggattc gctttacacg tttcgtaatt tctgttttcg cattcttcct
     2341 caacgatgaa atttgcgtac cgattggtat cctcaaccgg caccagcctg ttaaagctct
     2401 gaacgccgcg tccggcatct tcgtgtgcgc ggatttcccc cgcgcgccgc ccggtgtgga
     2461 tcagcaaagc ctccgcaccg ccgatgcgcc ccgccagcgc gatggattgg gtaagccccc
     2521 ggtttttgcc ggaataggcg gttttactct gaatgcccca ctgcctgcct tccccgataa
     2581 catcgtcggc ggttttggtt tgaaatgcga ccgagcccgc caatgcgccg ctgccttgtt
     2641 cgaccgagtt tgagcctttg ctgatttcga cagctttgac gttttcatac tcgatttcat
     2701 tgattgcgcc gctgctgccc gccgtcctcg tcccgcccaa tgccgcctgc gcggtgtagg
     2761 actgtatttg cgccaagccg tccaccgtca aggaaacgcg gtttttgtcc ataccgcgta
     2821 tcgagtagcc agagcttgcg ccgcgcccct gttcgaccac ggcgatgccg gggtcgtaac
     2881 gcgtcaggtc gcggatatcg agtacctgtt ccttgctgag ggtgtcggcg gttttgacca
     2941 atttgcccag accggttact tcgttatcgc ggcgggtttt ctgttttttg gcttttacct
     3001 gtatggtatc caactgtttt tcctgtgctt gtccggcttg cacattttct gcataaacgg
     3061 gcagcgcagt catcagcgac aggcataaaa tatttaatcg gaacaaatgt tgctgttgca
     3121 taatgtttcc ctaatcttcg ctttcagacg gcatcggaag gagcgatgcc gtcgaagcct
     3181 tattcttgat tgttcggcaa ccgtgcttat tgcacaggct gttggcgttt cgcaccgaat
     3241 acgacagttg cactgcttgc tgaatttcca ttgccggatg caactgttgt attttccgtt
     3301 tgtttatcgc ccgaataggc aaaccatccg cccaactctt cggctttagg tccgtaaaaa
     3361 ccgccctgca ccttggcgtt tgtgatatat gccttaggcg tgccggtggt attgctttga
     3421 tcgagatcaa aacctaattc agcagttttt gccgtacctt caaagccgtt gtccttaata
     3481 ttagccgtga tagtaaaggt tggctgagtc ctatcattag cggttaacgt gccggtaatt
     3541 tttttatcgc caaaattcac ggtaaagtcc gccctgttgc cactcgttgc attggaagca
     3601 ttgccgctcc agcttgtgct tgtgttgttg gcaatatgcc cgtaccaaga cccccgataa
     3661 acgatgtctt gttgctcttt tggaatctct ttttcatcgg tgcgctcgcc ttggaggaac
     3721 atactttgtt caacttgttc cgttttagcg tcagcttgac taccgttttc gcctgcctgc
     3781 atcgcggact tgctgttttt acgcgtcaac attccgtatt tcagataatt gaggttggaa
     3841 cagcagactt caacttcata ggtttttgtt cctgcctgcc cgccgttaac gcccgccgca
     3901 cccgaagcag cttgcgcgcc gcccgcgcct gtttgggctt tggtgtcttt tttatcactt
     3961 tccagcgtgt aggttgtttt gtaggtaaag gctgttccgc cgtttgtacc ttgattggct
     4021 tgatttttcc cactttcgga agcctcgggc aggagcggaa tcataatgcc gtcgacaacc
     4081 agttgggcgg cattgctgaa gttgtcgaga tttttgattg ctgtgccgcc gtgcgtcagc
     4141 tcgaccgcat ccaaaaccgt ggtcagctta ccgtttttag acgacgtgcc tgccgcaccg
     4201 tccgatgctg ccgcacctgt gccgcctgaa gccactgcgc catttctcgg tttgtcttgg
     4261 gttttcgcgc tgccgacaac ggcaactttt tgatcgttgc tcaaaaagcg gaaacccaat
     4321 tcctcaccct gcgggccgaa aaagccgccg ctcaaagaag acgagtcgga aacaaaggga
     4381 tgttgtttgg tttcattctc ttgtggtttg tcggtcgcta ttgccttgcc attgaagcgg
     4441 ttgcctgtta cttgagcctc aaggctgtag tattgcgtgg taggtttgtc gttattagta
     4501 ttactttggc tcgcattatt gcgtatcaat ttacccgtca atttcttatt gccgaaatcc
     4561 acttctaaat tcgaggtaaa tccataaccc tcgtgaccat ctttcagcgt ggattcgttt
     4621 ttgttggaat attcttcgcc ctcatcacca gaaaatccgc tgtagctgtc gccttgcttt
     4681 tttgaaggct ggataatatc gttaaatttt tgtcccagtt tcgtatcggt tacaaaatgc
     4741 catacgccct tgtaggtaac ttttccagaa gcaggaagtt gtcgggaagg gtctttaccg
     4801 tgataaaaga tatagccgtc gtcgcctgac ttaaattttc cttcattgaa ttttctttca
     4861 ctcgccgcat gtttataaaa ccaaccggaa taaacatatt tgaaattgtt gtaacctgtt
     4921 gcttcgtttt ttggttggtt tgcaccgcca ttgatactgc cgttgtgaga attatggctt
     4981 gattgcgtga gataagggga aatgtaaata ttgttgtcgc cattatctgt ttgtacgttt
     5041 gaaatgacgg attcttgttt taatggcagt ttcttgggtt ctgtcggcaa tcctgtcgcc
     5101 tcccaatcat cattctttaa tttaacctca ttttctttag gcattgccgt aggatgccga
     5161 ttcctccgtt tcaacctcat cgcaaaaccg tatccgcctt ggtctttttg ggcttgcggt
     5221 ttttcggaag aaacatcttg atactttggc gcgggacgcg gggcttcggt atcgacagaa
     5281 tcaagatcga agctgccgcc gccgcccaaa caagcactca acaaaaacac aggcagcacc
     5341 atagcagcct gatttaccaa tggattgttc atataaaccc aattcaatta aagaatgata
     5401 aggattatta ttttatttat ttttaacaaa tttgcaaata cttttttatt tttttaggga
     5461 atacaccaaa atcccgtcat tcccgcgcag gcgggaatcc gaacgcgtcc gcacggaaac
     5521 ctatatcccg tcattcccac gtatatcccg tcattcccgc gcaggcggga atc
//
LOCUS       DAWFLT010000108         5409 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWFLT010000108 DAWFLT010000000
VERSION     DAWFLT010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.108"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..1761
                     /locus_tag="WGR74_001960"
     CDS             <1..1761
                     /locus_tag="WGR74_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0906309.1"
                     /translation="LQSGWDTKLQGTQFETTLGGATIRAGVGEQARADAKIILEGIKS
                     SIHTETVSSSKSTLWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYIVDIPQGNLKT
                     QIETLTKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEGLTEAGAAIIALAVTVVT
                     SGAGTGAVLGLNGAAAAATDAAFASLASQASVSFINNKGNIGNTLKELGRSSTVKNLV
                     VAAATAGVADKIGASALNNVSDKQWINNLTVNLANAGSAALINTAVNGGSLKDNLEAN
                     ILAALVNTAHGEAASKIKQLDQHYIVHKIAHAIAGCAAAAANKGKCQDGAIGAAVGEI
                     VGEALVKNTDFSDMTPEQLDLEVKKITAYAKLAAGTVAGVTGGDVNTAAQTAQNAVEN
                     NAVKAVVTAAKVVYKVARKGLKNGKINVRDLKQTLKDEGYNLADNLTTLFDETLDWND
                     AKAVIDIVVGTELNRANKGEAAQKVKEVLEKNRPYIPNKGAVPNMSTYMKNNPFGKQL
                     AQISEKTTLPTQQGQSVFLVKRNQGLLKTGDRFYLDGQHKNHLEVFDKNGNFKFVLNM
                     DGSLNQMKTGAAKGRKLNLK"
     gene            1770..2147
                     /locus_tag="WGR74_001961"
     CDS             1770..2147
                     /locus_tag="WGR74_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906310.1"
                     /translation="METLNDIKKILINVGLYQGFDLTDPKVSEEVNHETANMKWIKDY
                     TSDGNWDNEFKEDLKNFLDYMEVCQLALNDKNFKIASNSLFMAMIYAGNLSLIFDSIK
                     TDISTLLSAEYKKNSFSWPSLDE"
     gene            2425..2799
                     /locus_tag="WGR74_001962"
     CDS             2425..2799
                     /locus_tag="WGR74_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906311.1"
                     /translation="MKNFNVVKESLRELGIKQGFDLYEKATTEKLNSEDPLDLQWLSN
                     YSSDWNDELEEDFDSFFQHMKEYQYAIDNEDIKSACSSLCEAMLYVGNIKNFFEFLKS
                     DMIRLLRGESKTTDFQWPQFDE"
     gene            2834..4429
                     /locus_tag="WGR74_001963"
     CDS             2834..4429
                     /locus_tag="WGR74_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0906312.1"
                     /translation="MRKVCASARTRSMIYGSMPSEKLTIFQTAFVMQVNIQIPYILPR
                     CVRAEDTPYACYLKQLQVTKDVNWNQVQLAYDKWDYKQEGLTGAGAAIIALAVTVVTA
                     GAGAGAALGLNGAAAAATDAAFASLASQASVSLINNKGDVGKTLKELGRSRTVKKLVV
                     AAAIAGVSNKIGASSLATWSETPWVNNLNVNLANAGSAALINTAVNGGSLKDNLEANI
                     LAALVNTAHGEAASKIKQLDQHYIAHKIAHAIAGCAAAAANKGKCQDGAIGAVVGEIV
                     GEALVKNTDFSGMTASEIEKAKANITAYAKLVAGATVGVTGGNVDVAANASETAVKNN
                     ALDIIWDIGNLVWDGGKWIYAKSIGDKQMAREAAIDFGVDAAAAAVPFVPAGATKISR
                     GGAYVLKAGDEAVDTAKAIQEIQKQTGIKLTYDKVNKVWTTPAGLDYGLDAKHGNRIK
                     HVLAHTIPNPNKPVHSVFNVSRKEVLPLVDEAWRMKGNPLPNDSSVYLVDMKKPIGTK
                     GETKVRIVVQKGTNKIISAYPQK"
     gene            4448..4711
                     /locus_tag="WGR74_001964"
     CDS             4448..4711
                     /locus_tag="WGR74_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906313.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            4818..5409
                     /locus_tag="WGR74_001965"
                     /pseudo
     CDS             4818..5409
                     /locus_tag="WGR74_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
BASE COUNT         1719 a         1084 c         1305 g         1301 t
ORIGIN      
        1 ctgcaatcag gttgggatac caaactgcaa ggcacacagt ttgaaaccac actgggtggc
       61 gcaaccatac gcgcaggcgt aggcgagcag gcacgggcag atgccaagat tatcctcgaa
      121 gggatcaaaa gcagcatcca cacagaaacc gtgagcagca gcaaatctac tctatggcaa
      181 aaacaggcag gacggggcag taacatcgaa accttgcaat tgccgagttt caccggtccc
      241 gttgcgcccg tactgtccgc acccggcggt tacattgtcg atattcccca aggcaatctg
      301 aaaacccaaa tcgaaaccct caccaaacag cccgagtatg cttatttgaa acaacttcaa
      361 gttgcgaaaa acatcaactg gaatcaggtg cagcttgctt acgataaatg ggactacaaa
      421 caggagggct taaccgaagc aggtgcggcg attatcgcac tggccgttac cgtggtcacc
      481 tcaggcgcag gaaccggagc cgtattggga ttaaacggtg cggccgccgc cgcaaccgat
      541 gcagcattcg cctctttggc cagccaggct tccgtatcgt tcatcaacaa caaaggcaat
      601 atcggtaaca ccctgaaaga gctgggcaga agcagcacgg tgaaaaatct ggtggttgcc
      661 gccgctaccg caggcgtagc cgacaaaatc ggcgcttcgg cattgaacaa tgtcagcgat
      721 aagcagtgga tcaacaacct gaccgtcaac ctggccaatg cgggcagtgc cgcactgatt
      781 aataccgctg tcaacggcgg cagcctgaaa gacaatctgg aagcgaatat ccttgcggct
      841 ttggtgaata ctgcgcatgg agaggcagca agtaaaatca aacaattgga tcagcactac
      901 atagtccaca agattgccca tgccatagcg ggctgtgcgg cagcggcggc gaataagggc
      961 aagtgtcagg atggtgcgat aggcgctgca gtcggtgaga ttgttggtga ggctttggtt
     1021 aaaaataccg attttagcga tatgaccccg gaacaattag atctggaagt taagaaaatt
     1081 accgcctatg ccaaacttgc ggcaggtaca gttgcaggcg taacgggagg agatgtcaat
     1141 actgctgcac aaaccgcaca aaacgcggta gaaaataatg cggttaaagc tgttgtaact
     1201 gctgcaaaag tggtttataa ggtagccaga aaaggattaa aaaacgggaa aatcaacgtt
     1261 agagatttaa aacagacgtt gaaagacgaa ggttataatt tagccgacaa cctgaccacc
     1321 ttattcgacg aaacattgga ttggaacgat gccaaagccg ttattgatat tgtcgtcgga
     1381 acagagctga atcgcgctaa taaaggggaa gcggcacaaa aggtcaagga agttttagaa
     1441 aaaaatcgtc cttatatccc taataaaggt gctgtaccga atatgagtac atacatgaaa
     1501 aataatcctt ttggaaaaca gctggctcaa atttcagaaa agacaacgct tccgacgcag
     1561 caagggcagt ctgtcttctt ggtaaaaaga aaccaagggt tattaaaaac cggtgatagg
     1621 ttttatttag atggccaaca taaaaatcat ttagaggttt ttgataaaaa tgggaacttt
     1681 aagtttgttc taaatatgga tggttcgctt aaccaaatga aaactggggc agcaaaaggt
     1741 cgtaaattaa acttaaaata ggaaatttta tggaaacatt gaatgatata aaaaaaatct
     1801 tgattaatgt ggggctttat caagggtttg atttgacaga tccaaaagta tcagaagaag
     1861 ttaatcatga aacagctaat atgaaatgga ttaaagatta tacttcagac gggaattggg
     1921 ataatgaatt taaggaggat ttaaaaaact ttttagatta tatggaagta tgccaattag
     1981 ccctaaacga taaaaatttc aaaattgcca gtaattcttt atttatggct atgatttacg
     2041 caggtaatct atctcttata tttgattcaa taaaaactga tatatcaaca ttattgagtg
     2101 ctgagtataa aaagaatagt ttttcatggc catctcttga tgaatagaaa gcaagttgta
     2161 gcctgcatga aatctaaaac ccatgcataa ggtgtgggct tcagtatacg cgttccatga
     2221 tttacggcca tatgccgtct gaaaagctca attttttcag acggcatttg ttatgaaagt
     2281 aaatatttag attccctgta tactgtttag actcgtgtgt gctgagtaag ctgtagtctg
     2341 catgaaacct aaaactcgct caaaattaag ctaagacatt agcagggcaa gggcgaaaat
     2401 tgaatcttaa ataaggtgat tcagatgaaa aattttaatg tagtaaaaga aagtttaaga
     2461 gagttaggaa ttaaacaagg atttgatctt tatgagaaag ccacaactga aaaattgaat
     2521 agtgaagatc ctcttgactt acaatggctt tctaactatt catctgattg gaatgatgaa
     2581 ttagaagaag actttgattc tttttttcag catatgaagg aatatcaata tgctattgac
     2641 aatgaagaca ttaaatctgc atgtagttca ctatgtgaag ctatgctcta tgttggtaat
     2701 attaaaaatt tttttgagtt tctcaaaagc gatatgatta gactgttgag aggtgaaagt
     2761 aaaacaacag actttcaatg gccgcaattt gatgaatagc agcaagctgt agcctgcatg
     2821 aaacctaaaa tccatgcgta aggtgtgtgc ttcagcacgc acgcgttcca tgatttacgg
     2881 ctcaatgccg tctgaaaagc tcacaatttt tcagacggca tttgttatgc aagtaaatat
     2941 tcagattccc tatatactgc ccagatgcgt gcgtgctgaa gacaccccct acgcttgcta
     3001 tttgaaacag ctccaagtca ccaaagacgt caactggaac caggtacaac tggcgtacga
     3061 caaatgggac tataaacagg aaggcttaac cggagccgga gcagcgatta ttgcgctggc
     3121 tgttaccgtg gttactgcgg gcgcgggagc cggagccgca ctgggcttaa acggcgcggc
     3181 cgcagcggca accgatgccg cattcgcctc gctggccagc caggcttccg tatcgctcat
     3241 caacaataaa ggcgatgtcg gtaaaaccct gaaggagctg ggcagaagcc gcacagtgaa
     3301 aaaactggtt gtagcagcgg ccatcgcagg cgtatccaac aaaatcggtg cttcctccct
     3361 tgccacttgg agcgaaaccc cttgggtaaa caacctcaac gtcaacttgg ctaatgcggg
     3421 cagtgccgcg ctgattaaca ccgccgttaa cggcggcagt ctgaaagaca atctggaagc
     3481 gaatatcctt gcggctttgg tgaatactgc gcatggagag gcagcaagta aaatcaaaca
     3541 attggatcag cactacattg cccataagat tgcccatgcc atagcgggtt gtgcggcagc
     3601 ggcggcgaat aagggtaagt gtcaggacgg cgcgattggt gcggtcgtgg gggagattgt
     3661 cggggaggct ttggttaaga ataccgattt cagcggtatg actgcttctg aaattgaaaa
     3721 agctaaagcg aatattactg cgtatgcaaa attggtagcc ggagcgactg taggtgttac
     3781 aggaggcaat gttgatgtgg cggcaaatgc ttccgaaaca gctgttaaaa ataatgcatt
     3841 agatattatt tgggatatcg gcaacctcgt atgggacggc ggtaaatgga tttacgccaa
     3901 atctattggc gataagcaga tggctcgaga agcggcgatt gattttggtg tggatgccgc
     3961 cgcagctgcc gttccctttg ttccggcagg tgcgactaaa atcagccgag gcggggctta
     4021 tgttctgaag gcgggagacg aagcagttga tacggctaaa gccatacagg aaattcagaa
     4081 gcagaccgga atcaagctta cttatgataa ggttaataag gtttggacaa caccggcggg
     4141 gttagattat gggttagatg ctaagcatgg taataggatt aaacatgttt tagcccatac
     4201 aattccaaat ccaaacaaac ctgttcattc tgtttttaat gtgtcccgta aagaagtttt
     4261 gcctttggtt gatgaagctt ggagaatgaa aggaaatcct ttgccaaatg attcatccgt
     4321 atatcttgta gatatgaaga aacctattgg aacaaaagga gaaacaaaag tgcggattgt
     4381 tgtgcaaaaa ggaacaaata aaatcatttc tgcatatcct cagaaataat taagaaagga
     4441 atctcttatg gataaagaaa ttaaaatttg cccaagatgt gagcaaggct acctttatca
     4501 tgcaaagcct aaatatttct ctggggaggt cattttatgc gatgaatgtt atgctatgtg
     4561 gcttggggat atgaaaattt tttacggaca atatggaaaa gatttttatg attatcatga
     4621 gtttatgaaa gataaaggca tagaagaaat aaatatgtgg gaaggagagc tttttgatca
     4681 cccatattat gaggatgaaa aatttaaata attgattttc tgttccccga atttgggaaa
     4741 tacgatgata ttttaaaccc aaatattatt taaagtagca ataggccgtc tgaatatccg
     4801 tttttcagac ggcctcaatg caactgctgg cagccgaagg cattcacaag cacgagttgg
     4861 atgtccaaaa aagccgccgc tttatcggca tcaaggtagg taagagcaat tacagtaaaa
     4921 acgaactgaa cgaaaccaaa ttgcctgtcc gcgtcgtcgc ccaaactgca gccacccgtt
     4981 caggctggga taccgtgctc gaaggtaccg aattcaaaac cacgctggcc ggtgccgaca
     5041 ttcaggcagg tgtaggcgaa aaagcccgtg tcgatgcgaa aattatcctc aaaggcattg
     5101 tgaaccgtat ccagtcggaa gaaaaattag aaaccaactc aaccgtatgg cagaaacagg
     5161 ccggacgcgg cagcactatc gaaacgctaa aactgcccag cttcgaaagc cctactccgc
     5221 ccaaattgtc cgcacccggc ggctatatcg tcgacattcc gaaaggcaat ctgaaaaccg
     5281 aaatcgaaaa gctgtccaaa cagcccgagt atgcctatct gaaacagctc caagtagcga
     5341 aaaacatcaa ctggaatcag gtgcagcttg cttacgacag atgggactac aaacaggagg
     5401 gcttaaccg
//
LOCUS       DAWFLT010000109         5364 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWFLT010000109 DAWFLT010000000
VERSION     DAWFLT010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.109"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(158..2992)
                     /gene="lbpA"
                     /locus_tag="WGR74_001966"
                     /gene_synonym="tbpA"
     CDS             complement(158..2992)
                     /gene="lbpA"
                     /locus_tag="WGR74_001966"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ0906314.1"
                     /translation="MNRRHTFPLTLTALAIAAAFPSYAANPETAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTYDILSPNKKAEYFLAEGESEL
                     KPVAKLAGNGNYLKNQLNRWVEERRSKGQALNSEEEAMVREAQARHENLSAQAYTGGG
                     RILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAKKF
                     SDKSGVYDGNDFRDGLYFRPNIEEWKGDTNLVKGIGLKYSRTKFIDEHHRRRRMGLLY
                     RYENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSS
                     DRFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISEST
                     GFDDDNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYF
                     DFSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQ
                     ELFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVAD
                     HKTKLPNQAGQLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNR
                     IKPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELA
                     YLAGDQKRYSAKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAE
                     STANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(2989..5148)
                     /locus_tag="WGR74_001967"
     CDS             complement(2989..5148)
                     /locus_tag="WGR74_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0906315.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTSPPAGPSVETTPVNRPAVGAAMRLPRRNIAAFDKNGNEIPDSKQAEEYLPLKEE
                     DILFLDGTPKEQADKLKKEINGRYPNVTIYTSDLKDAVYKYEHVRAGYVYTRFGTDEI
                     EQNSGGKQFSNRLGYDGFVYYSGERPSQSLPSAGTVEYSGNWQYMTDVKRHRAGKAVG
                     IDNLGYITFYGNDVGATSYAAKDVDEREKHPAKYTVDFDKKILKGELIKNQYVRDRNG
                     PYNPLTIYDITATLDGNRFTGSAKVNTKLKTSHADKEHLFFHDDADQRLEGGFFGDNG
                     EELAGRFISNDNSVFGVFAGKQKTDAANASDTKPALTSGKHTKILDSLKISVDEAADE
                     KARPFEVSTMPDFGHPDKLLVEGREISLLKDTQTIVLADGRTMTVSACCDFLTYVKLG
                     RIKTERPAVKPKAQDEEDSDIDNGEESEDEISEDDNGEDEVTEEEEAEETEEETDEDE
                     EEEPEETEETEETEETEETEETEETEETEETEEKSPTEEGNGGSGSILPTPEASKGRD
                     IDLFLKGIRTAEADIPQIGKARYTGTWEARIGEPIQWDNKADKAAKAEFNVDFGEKSI
                     SGTLTEENGVEPAFHIENGKIEGNGFYATARTRDNGINLSGTGSTDPKTFKANDLRVE
                     GGFYGPKAAELGGNIIDSDRKFGAVFGAKKDDKEATR"
BASE COUNT         1127 a         1458 c         1263 g         1516 t
ORIGIN      
        1 gataaattgc cttagcattg aatgtctgga ttcccgcctg cgcgggaatg acggcggaaa
       61 tcttgtttat attgaatcaa aaaaacctgc accttaatca gttggcggtt tagtccgact
      121 tttggggtgc agatcaagct ttcagacggt atttccttta aaacttcatt tcgagcgcga
      181 ggctgaagtt tctgcccggt gcggcatacc ttccatagtt actgtcgccg ccgtgccggt
      241 ttgccgtgct ttccgcagtc tggcgcaagg attcccaagt aacgtagcgg tagttgccga
      301 tattgtagat agccgccctc aaggtcagcc gttttttcag attcagatag gcggaaacgt
      361 ctgccgtcga ccaagaagac gacgctcttt ttgccgaata tcgtttttga tcgcctgcca
      421 gataagcaag ctcgtcaggg tttttccctt tggaataggt cagcataatg tttgcgcccc
      481 atttcccctc gggctggtcg tatccgaacc ccaaaacata acgcgacggc tgtaccgcat
      541 ccaaagcata gctgcggagg gacagtcccg gccggttgga taccgatttc ggtttgatgc
      601 ggttgtacgc caaagtcgtg tacaaacctt cgggcagttt gccgtacaca ccgttccagt
      661 cgatttttcc caatatatta acgccttgaa gcgacatatt ttgggcattg taataatcgc
      721 gtatatcaat ctctgtcaat tgtcctgcct gattcggcaa tttggttttg tgatcggcaa
      781 cggcaatcat atcggtatag cggttgcgga agctgctgat ttccaaaaag ccgaagtcgc
      841 ctttccactg caaaccgatt tcccggttgg ctgccttttc cgatttcagg gcgggacgct
      901 gccagccttt cggataatcg tgataaatgt ctatcccgaa aagttcttgg aatgagggcg
      961 ttctgaagcc gctggaggcg cggtaagaca cggaaaaatg ccggttcggt ttgaacacga
     1021 tgccgctgtt ccacgaacgg tcaacatacc gtccgctgcg gacgagttct tccgacgtgg
     1081 tgaagttttt ccggtcatac ctgccgccca agctgaaatc gaaatatttg ccgattgaaa
     1141 aacggtcgtt caaagaaatg tggatattgc tgccgttgat ttttcttggc acgcatttgc
     1201 gggaacgcag ggtttcgatg tagccgcaga ccgacccttc gacgacttcg ggcttaccca
     1261 aacggtactt atcttgattg tcatcatcga atcccgtgga ttccgaaatc cttgccgcat
     1321 tgtgggaaag ctgttcgggg cgggagatcg ctttggaagc atcgtaaccg aagcccaaag
     1381 tcagatggtg tttcgtccat ttgtttttca gcgatttctc aaacgaggca ttcaaaacat
     1441 tgtgctgttc gcggtagtgg aaacggtcgc tgctgtcgta ggaatacggt ttgtccgccg
     1501 acgcgcggca ggatttgtcc acagcaggat acacggcgca attcagcttc agcgtgttgt
     1561 tgtcggttgc cacgccctgt ttgtcaaacg acaacaccgc cttatccgcc cagttgtcag
     1621 aatacgcttc gttttcataa cggtacagca aacccatacg gcggcggcgg tgatgttcgt
     1681 ctataaattt ggtgcgggaa tatttcaaac ctatgccctt gaccaaattt gtatcgccct
     1741 tccactcttc tatattcggc ctaaaataca agccgtcgcg gaaatcgttg ccgtcgtaca
     1801 ccccgctctt gtcgctaaac tttttcgcct catccgtacc gtaatactgt ttttccgtca
     1861 tatcgcggat gtcgtaacgc tgtttggtat cctcaaacac gccgccgaca taatgcctgc
     1921 cgccgaagcg gtagcccagc ttggcaagcc acgaaccgct gcggtaatcc atcggatcgg
     1981 gcaatatcct gccgccgccc gtgtaagctt gggcggacag gttttcgtgg cgcgcctgcg
     2041 cctcccgcac catcgcttct tcttcactgt ttaaagcctg accttttgaa cggcgttctt
     2101 ccacccagcg gttgagctgg tttttcaaat agtttccgtt gcccgccaat tttgccacag
     2161 gcttgagctc actctcgccc tccgccagga aatattccgc ctttttattc ggactcagaa
     2221 tatcgtatgt ctggcggaac gcgtccaaac ggtttatgcc gtatgccacc ccgtccgcaa
     2281 tatcgccgtg cgggcgcgtt tcccgccctt ggcgttcggt tcggattagc agcccttccc
     2341 aaccgtcttt gctgaacccc gcgccgagcg acttcataaa ttggcggttt ttactgccgt
     2401 aggcagtttt tgcctgtatc ccccaacttt tgccgtctga aatcaggtct gccgcttctt
     2461 tggtgcggaa ggcgaccgcg ccgccgagtg cgccgctgcc gtgatcggac gaaccggcgc
     2521 ctttgtcgat ttccaccgtg ctgatgtttt catattcgat ttcgttgatt gcaccgctgc
     2581 cgccgcgtcc gccgtatccg ctcaacgatc cctgcacggt aaacgcctgt atttgggcaa
     2641 cgccgtcaac cgaaaccgcc acacggtttt tatccacgcc gcgtatcgag tagccgccgc
     2701 tcgcgccgtt gccctgttcg acaaccgcca cgcccggatc gtagcgcgtc aggtcgcgga
     2761 taccgagtac ctgttctttg ttcaacgttt ccgacgtttt gacgattttg cccaaaccgg
     2821 tcgcctcttt cgatcgccgt cccactttgg cggcacggac ggtaacctct ttcaaggatt
     2881 gggtctgcgc cgcatcaggt gccgccgttt cagggtttgc cgcatatgac gggaaggcgg
     2941 cggcaatggc caaggcggtc agggtaagcg ggaatgtatg ccttctgttc atcgtgttgc
     3001 ctccttgtca tctttcttcg caccgaatac cgcaccgaat ttccggtcgc tgtcgataat
     3061 attaccgccc aattccgccg ccttcggacc gtaaaatcct ccttctacac gaagatcatt
     3121 agctttgaag gttttggggt cggtcgaacc agttcccgaa agattgatgc cgttatcccg
     3181 agtgcgcgct gtcgcgtaga aaccgttgcc ctcaatcttg ccgttttcaa tatggaaagc
     3241 aggttctaca ccgttttcct ccgtcagcgt tccggaaatc gatttctcgc cgaaatcaac
     3301 attaaactct gcttttgccg ctttatccgc cttattgtcc cattgaatgg gttcgccgat
     3361 acgcgcttcc caagtgccgg tatagcgtgc ttttccaatt tgcggaatgt cggcttccgc
     3421 cgtgcggata cctttcagga aaaggtcgat gtccctgcct ttagaggctt ccggagtggg
     3481 caggatgctg cctgaaccgc cgttgccttc ttctgtcggc gatttttctt cagtttcttc
     3541 agtttcttca gtttcttcag tttcttcagt ttcttcagtt tcttcagttt cttcagtttc
     3601 ttcagtttct tcgggttctt cctcttcgtc ttcatcagtt tcttcttcgg tttcttcagc
     3661 ttcctcttct tcggtgactt catcttcgcc gttatcatct tcggaaattt cgtcttcgct
     3721 ttcttcgcca ttatcaatgt ccgaatcctc ttcatcctgc gccttcggtt tgacggcggg
     3781 gcgttcggtt tttatccgtc cgagtttcac ataggtcaga aaatcgcaac aagcactgac
     3841 ggtcattgtc ctaccgtcgg caagaacgat ggtttgagta tctttgagca aggagatttc
     3901 acgcccttcg acaagaagtt tgtcgggatg accaaaatcg ggcatagtgg aaacctcaaa
     3961 cggacgggct ttttcatcag ccgcctcgtc aacggaaatt tttaaagaat ccaagatttt
     4021 ggtgtgtttt ccagacgtca gggcaggttt tgtatctgat gcgtttgctg cgtctgtttt
     4081 ttgtttgcct gcgaatacgc cgaatacgct gttgtcgttg ctgataaacc gtccggcaag
     4141 ctcttctccg ttatcgccga aaaaaccgcc ctcaagccgc tgatcggcat cgtcatggaa
     4201 aaacaaatgc tctttatcag cgtggctcgt cttcaacttg gtgttaactt tggcactgcc
     4261 ggtaaagcgg ttgccgtcca atgttgcagt aatgtcgtaa atggtcagcg gattataggg
     4321 accatttcta tctcgcacat actgattttt aatcagctcg ccctttagga tttttttgtc
     4381 gaaatcaacc gtatatttgg caggatgctt ttccctttcg tcgacatcct tagccgcata
     4441 agaagttgca ccaacatcgt taccataaaa tgtgatataa cccaaattgt caatgccaac
     4501 cgccttacct gctcgatgac gtttgacatc ggtcatatat tgccagttac cagaatattc
     4561 caccgttccc gcactcggta aagattggga aggacgttct ccggaataat atacaaaacc
     4621 gtcataacct aagcggttgg aaaactgctt accacctgag ttctgttcga tttcatctgt
     4681 tccaaatcta gtataaacat atccggcccg gacatgttca tatttataca cagcatcttt
     4741 taaatcggac gtgtagattg ttacattagg ataccgtccg ttgatttcct ttttaagttt
     4801 gtcagcctgt tctttcggcg taccgtctaa aaacaggata tcctcctctt tgagcggcag
     4861 atactcctct gcctgcttac tatcgggaat ttcattacca tttttatcaa aagctgcgat
     4921 attccgcctt ggcagccgca ttgccgcacc gacggcgggc cggttgaccg gcgtggtttc
     4981 taccgaaggc ccggcagggg gcgaagtggg aacgtcctta gacttgaaag tgacggggta
     5041 cgcggtcggc gttgattcga caacaggctg cacgccgaaa ttgccgccga tgcaagatgc
     5101 caaaagtaag ggcaacaaga caatgccgcc ataattcggt ttacacatcc ctacttttcc
     5161 tcctatttga ttaataataa ttatcattat attaatatgt acagataata tcaacccgtt
     5221 tttatagtga attaacaaaa atccggacaa ggcgacgagc cgcagacagt acagatacat
     5281 cccgtcattc ccacgaacct acaccacgtc attcccacga acctacattc cgtcattccc
     5341 acgaacctgc accacgtcat tccc
//
LOCUS       DAWFLT010000110         5308 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWFLT010000110 DAWFLT010000000
VERSION     DAWFLT010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.110"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(90..1445)
                     /locus_tag="WGR74_001968"
     CDS             complement(90..1445)
                     /locus_tag="WGR74_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ0906316.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINIGSTRAGINM
                     DAVRLAGETIKRTAEITLEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(1460..1732)
                     /locus_tag="WGR74_001969"
     CDS             complement(1460..1732)
                     /locus_tag="WGR74_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ0906317.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCSKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(1858..2595)
                     /locus_tag="WGR74_001970"
     CDS             complement(1858..2595)
                     /locus_tag="WGR74_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ0906318.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIHHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(2876..3775)
                     /gene="prmB"
                     /locus_tag="WGR74_001971"
     CDS             complement(2876..3775)
                     /gene="prmB"
                     /locus_tag="WGR74_001971"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0906319.1"
                     /translation="MFNQAAQELTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            3920..4513
                     /locus_tag="WGR74_001972"
     CDS             3920..4513
                     /locus_tag="WGR74_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906320.1"
                     /translation="MADGMGNLIGWEKTGLVAGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGTLTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHATSKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPDIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDGHKHYDHFILKDE"
     gene            4625..5062
                     /locus_tag="WGR74_001973"
                     /pseudo
     CDS             4625..5062
                     /locus_tag="WGR74_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT         1451 a         1364 c         1182 g         1311 t
ORIGIN      
        1 attcttttct aaagacattt tacccgatgc ctataaacaa aaatgccgtc tgaagccttt
       61 cagacggcat ttccccatca aaaccgcaat cagtttttca tcgattgaac cggtgcggga
      121 atcctgccgc cccggttgac gaacacttcg catgagcctt cttttaccgg cattacaggc
      181 gcgtagccca acaggccgcc gaactcgacg ctgtcgccga cggttttacc ggttaccgga
      241 ataatgcgca cggcagtggt tttgctgttg atcatgccga tggcggcttc gtcggcaatg
      301 atgccggaaa tggtgtgcgc gggtgtgtcg ccgggaacgg caatcatatc caagccgacc
      361 gaacaaacgg cggtcatcgc ttcgagttta tccaacgtca gcacgcctgc ttcggcggcg
      421 gcaatcatac cttcgtcttc ggaaacgggg ataaacgcgc cactcaaacc gccaaccgcg
      481 ctcgaagcca tcatgccgcc ctttttcacg gcatcgttca gcaatgccaa agctgctgtt
      541 gtgccgtgcg taccgcagac gctcaaaccc atttcttcaa gaatgcgcgc caccgagtcg
      601 ccgacggcag gggtcggtgc cagcgacaag tcgagaatac caaacgggat attcagcatt
      661 tttgaggctt cgcggccgat gagttcgccc acgcgggtaa ttttgaaggc ggttttcttc
      721 acaacttcgg caacttcggt caatgtcgtt gcatccgaat tttccaacgc ggcttttacg
      781 acacccgggc cggatacgcc gacattaatc acagcatccg cttcgcctga gccgtgaaac
      841 gcgcccgcca taaacgggtt gtcttccacc gcgttgcaga acacgacgat tttggcgcag
      901 ccgaaacctt ctagtgtgat ttcagccgtg cgtttgatgg tttcgcccgc cagtctgacc
      961 gcgtccatat tgataccggc gcgcgtactg ccgatattga tggagctgca cacgatatca
     1021 gtagtcttca tcgcttcggg aatggaacgg attaacacct cgtcagaagg cgacatacct
     1081 ttttgcacca gcgcggaaaa gccgccaata aaagacacgc cgatggcttt ggcagcctta
     1141 tccaaagttt gcgccacgct gacgtaagaa tcagcatggg tggccgccgc gatttgggca
     1201 atcggcgtga cagaaatgcg ctgattcaca atcggcacgc cgtatttggc agacagatat
     1261 tttgctgtcg ccaccaagtc tttgccgacc gtggtaattt tgttgtaaat attttggttc
     1321 aacacgtcga tgtcggtgct gatgcagtcg tgcaaatcaa tgccgatggt aatggtgcgg
     1381 acatcgaaat tctggtcggc aaccattttg acggtttcta aaatttcgcc ggattggata
     1441 ctcatcacat tcctccggct caaatgcggt gcatcgcttg gaagatttct tcgttttgca
     1501 tacggatgtc gagcgcgagt tttttgctct cttccgcaaa caaatccaaa acctcctgcc
     1561 gcgatttgct gcattttgaa gtgtccacca agataatcat ggtaaaaaaa tcatccatca
     1621 gctgttggct gatattgagg atgttgattc ggttttccgc caaaattttg gaaacatcgt
     1681 acacgatgcc gacgcggtct ttaccgatga cggtgatgac tgaattgttc acaggcttac
     1741 tccttgcaga tatccgttaa agtccgaaat tataccaccg ttggattttg aagaaatatt
     1801 gtcaacaata tatacataca aaatgccgtc tgaaactatt tcagacagca tcaagattca
     1861 gggttcgatt aaataaccat ccttatccca ctgggttttc ctgaccaact tatcatcctg
     1921 ataaacagct tcactctttt tagaaccatc ttcataccac tctaagacta ctccattacg
     1981 tttatgatga tggatagaca gttccgagag taatcgaccg ctttcatccc atgtcagaat
     2041 tttggcaggc tcatcattga ccataaccat ttccgttttg atattgccgt cggcatacca
     2101 ttgcttccat acgccgtttg ccttattctg tttaaactgg atttcgcttt ccttgccgcc
     2161 gttacggtaa tagcggtacc ccgtaccttc actcaaacca tttttataag gcataacggc
     2221 agatttttta ccgttcggat accagttgac ccactccccg tccggcttac ccttgctgaa
     2281 gccccccgcc atttttttct gaccattaaa atgccacaaa atcaacatac cgttttgcag
     2341 ggtaggcaca aaagatttga tttgcgttga agcaacgata taaggttcgg aatatttctt
     2401 catcgacgga taataaaaat cctgcgcgtg cgcaataccc tccgccacac tatattgcct
     2461 gatataagcg gcagaagaca tcgtcgccgt cagtttcccg ttctgattaa aataaacaga
     2521 ataggtctgc gccggcaaag cggccgaaaa acccaacagg acagttgaaa atacaatccg
     2581 agataatttt ttcattgcaa tagcgatata aaaacaaggc tgtgttttag taatctgttg
     2641 atttcaatta tttgcaaggg aaaagacaat tattttccgg ttaggaataa acctattcta
     2701 ttgaatagat tgaagccaag tacgcctatc aacactatat taaaacactg ccaaaaacaa
     2761 ttaacttata aacaatatgg taaggatttc tctgccaagc atcaaacccg agacaacgta
     2821 tcgtaaaaat gccgtctgaa aacaaatcgt cttcagacgg catttccctt tcaactcact
     2881 cttcacccaa taactgctcg cgcgtcaaga ggaaaacaaa accgtcgccc ccgctggttt
     2941 ccaaccaagt aaaaggcaac tccggatacg ctgcttccaa tacatccctg ttatgcccga
     3001 tttccaccag caatacacct ttgggattca gaaactttgc cgcattcaga agaatctgcc
     3061 tggtggcatc caacccgtcc gccccgctgc ccaatgccaa ttccggttcg tgcaaatact
     3121 cttcaggcaa taactcaacc gattccgcat ccacataagg cggattggaa acaatcaaat
     3181 cataagtgcc ttccaatcct tcaaacaaat ccgtatgaat aagccggatg cgttcttcca
     3241 aaccataatc ttcgacatta atccctgcca cttccaaagc atccaagctc acatcaaccg
     3301 catcaatttg ggcatcagga taatgatgcg ccatctgaat ggcaaggcaa ccgcttccgg
     3361 tgcaaagatc caaagcatta tgcaccaact catcgtattc tatccaaggg cgaagtccgt
     3421 cacccaacaa ttcataaata aaagaacgag gtatgattac gcgctcatcc acatagaaat
     3481 caaactctcc ctgccatgcc tggtgtgtca aataagcggc tggaatgtgt tcgacagcac
     3541 gacgctcaat aaccgccagc acttcctctt tttcagcttc caagagtttt gcatcaagat
     3601 atggggcaag catatccaaa ggcaaattca aagtatgcag aatcaaataa gccgcttcat
     3661 catgcgcatt atctgttcca tgaccaaaaa agagccctgc ctcattaaaa cggctgactg
     3721 caaaacgtaa aatatcgcgg atagtcgtca attcttgtgc tgcctgatta aacataatat
     3781 gaaccattct gcgtatagat acttttaatt ataacagaaa caacaagcaa accttttcat
     3841 atcgccaaat aaccacccaa tctacccata caactacata aatgcccgag cgaaaaccat
     3901 cgcccgaacg gaaacgacaa tggccgacgg tatgggcaat ctgattggct gggaaaaaac
     3961 ggggcttgtt gccggtaagc agtggataac cgcaaaagac gacaaggtgt ccgatgtctg
     4021 caatgccaac ggcgagatgg gcgtaatcgg gctttacgag cctttctcac acggcacatt
     4081 gacgataccc ggtcatccga actgccgatg cgaggttgtt tccgtatcgg gtggcgaatt
     4141 gggggaattt gccgaaaaaa aggagcttcg taaagcggct atgcagtatg cgcgggataa
     4201 ctttatcggc aaaagctatg tcaatgaaaa cagcgggcat gaactgaagg taacttggca
     4261 aggtgtgaaa cacgctacgt caaaggcaaa tcaggcggaa ttatccatca tgacaaaact
     4321 tgatgactta ttgcgctacg caaaatatga gggttcttat tcggatagga aaggtcatcc
     4381 tgatattatt gcagcacata agtatcgtgc cgttgccaag gttgggaatg agtctttaaa
     4441 tatcggtgtg attgtaaggg aatttccaga cggacataaa cattacgacc atttcatctt
     4501 gaaggatgag taaagccctt ttgcagtgtc gttctggagc ggatagcgtt aaggcaagta
     4561 cgcttccagc cttacaaaag ggctttaaat tcagcatgcc atttatacag gcaggagtaa
     4621 acccatgaca aagttatacg cagaaatcgc caagatggag acgcaggacg acggcacggt
     4681 caaggtttgg ggttacgctt caagcgagga aatcgattcg gacggcgaag tcatcgcggc
     4741 ggcagctatg aaggcggcga ttcccgatta tatgaagttt ggcgcggggc gcgagatgca
     4801 cggctcaaac gctgcgggaa cggcaattga aatcaacgtt gaagacgacg gcataacctt
     4861 tttcggtgct catatcatcg accccgttgt cgtgtcgaaa gtcaaaacag gcgtttacaa
     4921 aggcttttcc atcggcggca gcgttaccgc ccgcgatgat ttgaacaagt cgcaaatcac
     4981 gggcttgaag ctgacagaaa tcagccttat cgaccgccct gccaatcccg atgcggtgtt
     5041 tacctgcttt aaggcggaca aaggtgcgga agcggtaaac aacgatacag aacataatgc
     5101 tacatatttt agccatttcc cttccaaaca aaaaacaaaa aagcaccggc ggcagccgat
     5161 gccccttcct ttacaggttc ccctattttt tcactgcggg cagcactggt ttggctgggc
     5221 cttttggtgc gggcgcgccg acggaagcct gatccttcag cttcgccagc accgcagggc
     5281 cgatgccctt taccttggtc aaatcgtc
//
LOCUS       DAWFLT010000111         5306 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWFLT010000111 DAWFLT010000000
VERSION     DAWFLT010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.111"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..317)
                     /locus_tag="WGR74_001974"
     CDS             complement(<1..317)
                     /locus_tag="WGR74_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEZ0906321.1"
                     /translation="MLCPEKMSGMAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQELEALPGIGPAKAKAIAE
                     YRA"
     gene            complement(353..466)
                     /gene="rrf"
                     /locus_tag="WGR74_001975"
     rRNA            complement(353..466)
                     /gene="rrf"
                     /locus_tag="WGR74_001975"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(563..3454)
                     /locus_tag="WGR74_001976"
     rRNA            complement(563..3454)
                     /locus_tag="WGR74_001976"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3861..3936)
                     /locus_tag="WGR74_001977"
     tRNA            complement(3861..3936)
                     /locus_tag="WGR74_001977"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3901..3903),aa:Ala,seq:tgc)
     gene            complement(3942..4018)
                     /locus_tag="WGR74_001978"
     tRNA            complement(3942..4018)
                     /locus_tag="WGR74_001978"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3982..3984),aa:Ile,seq:gat)
     gene            complement(4122..>5306)
                     /locus_tag="WGR74_001979"
     rRNA            complement(4122..>5306)
                     /locus_tag="WGR74_001979"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1150 a         1553 c         1147 g         1456 t
ORIGIN      
        1 tgcgcacggt attccgcaat ggccttcgcc ttcgcagggc ctatgcccgg cagcgcctcc
       61 agctcctgct gcgaagccgc attgatgttt accgccgcaa gggagaaggc gcaggagaac
      121 agcatacaga acagcacgaa cattttcttc atggtttttc ctttaagggt tgcaaacaat
      181 aaaccgcatc ttgcgacgat aaaacgggtc attctaaaat gaatatccca aagtttcaag
      241 ccgttcctcc gcaaacccga ccggacgccg tacggatgcc gtcccgccat ccccgacatt
      301 ttttccgggc aaagcaaaaa cccccgaata atcgggggtt ttctgaatgg gtgtttggca
      361 gtgacctact ttcgcatgga agaaccacac tatcatcggc gctgagtcgt ttcacggtcc
      421 tgttcgggat gggaaggcgt gggaccaact cgctatggcc gccaaactta aactgttaca
      481 aatcggtaaa gccttaatca atatattcgg taatgactga atcagtcagt aagcttttat
      541 ctcttgaagt tcttcaaatg atagagtcaa gcctcacgag caattagtat gggttagctt
      601 cacgcgttac cgcgcttcca caccccacct atcaacgtcc tggtctcgaa cgactcttta
      661 gtgcggttaa accgcaaggg aagtctcatc ttcaggcgag tttcgcgctt agatgctttc
      721 agcgcttatc tcttccgaac ttagctaccc ggctatgcaa ctggcgttac aaccggtaca
      781 ccagaggttc gtccactccg gtcctctcgt actaggagca gcccccgtca aacttccaac
      841 gcccactgca gatagggacc aaactgtctc acgacgtttt aaacccagct cacgtaccac
      901 tttaaatggc gaacagccat acccttggga ccgactacag ccccaggatg tgatgagccg
      961 acatcgaggt gccaaactcc gccgtcgata tgaactcttg ggcggaatca gcctgttatc
     1021 cccggagtac cttttatccg ttgagcgatg gcccttccat acagaaccac cggatcacta
     1081 tgtcctgctt tcgcacctgc tcgacttgtc ggtctcgcag ttaagctacc ttttgccatt
     1141 gcactatcag tccgatttcc gaccggacct aggtaacctt cgaactcctc cgttacgctt
     1201 tgggaggaga ccgccccagt caaactgcct accatgcacg gtccccgacc cggatgacgg
     1261 gtctgggtta gaacctcaaa gacaccaggg tggtatttca aggacggctc cacagagact
     1321 ggcgtctctg cttctaagcc tcccacctat cctacacaag tgacttcaaa gtccaatgca
     1381 aagctacagt aaaggttcac ggggtctttc cgtctagcag cgggtagatt gcatcttcac
     1441 aaccacttca acttcgctga gtctcaggag gagacagtgt ggccatcgtt acgccattcg
     1501 tgcgggtcgg aacttacccg acaaggaatt tcgctacctt aggaccgtta tagttacggc
     1561 cgccgtttac tggggcttcg atccgatgct ctcacatctt caattaacct tccagcaccg
     1621 ggcaggcgtc acaccctata cgtccacttt cgtgttagca gagtgctgtg tttttaataa
     1681 acagtcgcag ccacctattc tctgcgaccc tccggggctt acggagcaag tccttaacct
     1741 tagagggcat accttctccc gaagttacgg tatcaatttg ccgagttcct tctcctgagt
     1801 tctctcaagc gccttagaat tctcatcctg cccacctgtg tcggtttgcg gtacggttcg
     1861 attcaaactg aagcttagtg gcttttcctg gaagcgtggt atcggttgct tcgtgtccgt
     1921 agacactcgt cgtcacttct cggtgttaag aagacccgga tttgcctaag tcttccacct
     1981 accggcttaa acaagctatt ccaacagctt gccaacctaa ccttctccgt ccccacatcg
     2041 catttgaatc aagtacagga atattaacct gtttcccatc gactacgcat ttctgcctcg
     2101 ccttaggggc cgactcaccc tacgccgatg aacgttgcgc aggaaacctt gggctttcgg
     2161 cgagcgggct tttcacccgc tttatcgcta ctcatgtcaa cattcgcact tctgatacct
     2221 ccagcacact ttacaatgca ccttcatcgg cctacagaac gctcccctac catgccggta
     2281 aaccggcatc cgcagcttcg gttatagatt tgagccccgt tacatcttcc gcgcaggacg
     2341 actcgaccag tgagctatta cgctttcttt aaatgatggc tgcttctaag ccaacatcct
     2401 ggctgtctgg gccttcccac ttcgtttacc acttaatcta tcattgggga ccttagctgg
     2461 cggtctgggt tgtttccctc ttgacaacgg acgttagcac ccgctgtctg tctcccgagg
     2521 aaccacttga tggtattctg agtttgccat gggttggtaa gttgcaataa ccccctagcc
     2581 ataacagtgc tttaccccca tcagtgtctt gctcgaggca ctacctaaat agttttcggg
     2641 gagaaccagc tatctccgag tttgtttagc ctttcacccc tatccacagc tcatccccgc
     2701 attttgcaac atgcgtgggt tcggtcctcc agtacctgtt acggcacctt caacctggcc
     2761 atggatagat cactcggttt cgggtctaca cccagcaact catcgcccta ttaagactcg
     2821 gtttccctac gcctccccta ttcggttaag ctcgctactg aatgtaagtc gttgacccat
     2881 tatacaaaag gtacgcagtc acacctctag ggcgctccca ctgtttgtat gcatcaggtt
     2941 tcaggttctg tttcactccc ctcccggggt tcttttcgcc tttccctcac ggtactggtt
     3001 cactatcggt cgatgatgag tatttagcct tggaggatgg tccccccata ttcagacagg
     3061 atttcacgtg tcccgcccta cttttcgtac gcttagtacc actattgaga tttcgaatac
     3121 gggactatca cccactatgg tcaagcttcc cagcttgttc ttctatctcg acagttatca
     3181 cgtacaggct cctccgcgtt cgctcgccac tacttgcgga atctcggttg atttcttttc
     3241 ctccgggtac ttagatggtt cagttctccg ggttcgcttc tctaagtcta tgtattcaac
     3301 ttaggatact gcacagaatg cagtgggttt ccccattcgg acatcgcgga atcattgctt
     3361 tattgccagc tcccccgcgc ttttcgcagg cttacacgtc cttcgtcgcc tatcatcgcc
     3421 aaggcatcca cctgatgcac ttattcactt gactctatca tttcaagaac ttctttgact
     3481 ttgcctaaca ttccgttgac tagaacatca gacttgaatt tcctactttg ataaagctta
     3541 ctgctttgtt gtgtcttaat cctgcctttt gtgtttcagg attaagtcga tacaatcatc
     3601 acccaaatac tgtgtttgtt ttcttttctc ttgtgagcga ttttttatcc tttgcaaaga
     3661 ataaaaaatc aaaacaaacg ctttgtcttt gtttgttgat ttcggctttc caatttgtta
     3721 aagatcgatg cgttcgatat tgctatctac tgtgcaaatc aaaacgagct gattattata
     3781 tcagcatttt gttcttggtc aagtgtgacg tcgccctgaa tggattctgt tccattcttc
     3841 cgttttgatt tgtacagtat tggtggaggc aaacgggatc gaaccgatga ccccctgctt
     3901 gcaaagcagg tgctctacca actgagctat gcccccgttc ttggtgggtc tgggaggact
     3961 tgaacctccg accccacgct tatcaagcgt gtgctctaac cagctgagct acaaacccgg
     4021 attctctaat cttaagcgaa tcttgccttc actcaagctt cttccgcatc tttttcagtt
     4081 taccgataag tgtgaatgcc taaagcctct tctttctcta gaaaggaggt gatccagccg
     4141 caggttcccc tacggctacc ttgttacgac ttcaccccag tcatgaagca taccgtggta
     4201 agcgggctcc ttgcggttac cctacctact tctggtatcc cccactccca tggtgtgacg
     4261 ggcggtgtgt acaagacccg ggaacgtatt caccgcagta tgctgacctg cgattactag
     4321 cgattccgac ttcatgcact cgagttgcag agtgcaatcc ggactacgat cggttttgtg
     4381 agattggctc cgcctcgcgg cttggctacc ctctgtaccg accattgtat gacgtgtgaa
     4441 gccctggtca taagggccat gaggacttga cgtcatcccc accttcctcc ggcttgtcac
     4501 cggcagtctc attagagtgc ccaactgaat gatggcaact aatgacaagg gttgcgctcg
     4561 ttgcgggact taacccaaca tctcacgaca cgagctgacg acagccatgc agcacctgtg
     4621 ttacggctcc cgaaggcact cctccgtctc cggaggattc cgtacatgtc aagaccaggt
     4681 aaggttcttc gcgttgcatc gaattaatcc acatcatcca ccgcttgtgc gggtccccgt
     4741 caattccttt gagttttaat cttgcgaccg tactccccag gcggtcaatt tcacgcgtta
     4801 gctacgctac caagcaatca agttgcccaa cagctaattg acatcgttta gggcgtggac
     4861 taccagggta tctaatcctg tttgctaccc acgctttcgg gcatgaacgt cagtgttatc
     4921 ccaggaggct gccttcgcca tcggtattcc tccacatctc tacgcatttc actgctacac
     4981 gtggaattct acctccctct gacacactcg agtcacccag ttcagaacgc agttcccagg
     5041 ttgagcccgg ggatttcaca tcctgcttaa gtaaccgtct gcgcccgctt tacgcccagt
     5101 aattccgatt aacgctcgca ccctacgtat taccgcggct gctggcacgt agttagccgg
     5161 tgcttattct tcaggtaccg tcatcagccg ctgatattag caacagcctt ttcttccctg
     5221 acaaaagtcc tttacaaccc gaaggccttc ttcagacacg cggcatggct ggatcaggct
     5281 tgcgcccatt gtccaaaatt ccccac
//
LOCUS       DAWFLT010000112         5157 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWFLT010000112 DAWFLT010000000
VERSION     DAWFLT010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.112"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(1..252)
                     /locus_tag="WGR74_001980"
                     /pseudo
     CDS             complement(1..252)
                     /locus_tag="WGR74_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
     gene            complement(324..569)
                     /locus_tag="WGR74_001981"
     CDS             complement(324..569)
                     /locus_tag="WGR74_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906322.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILRPLYIASLRRDD"
     gene            complement(570..866)
                     /locus_tag="WGR74_001982"
     CDS             complement(570..866)
                     /locus_tag="WGR74_001982"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906323.1"
                     /translation="MLLKVVPERTVSADAKTRDPMWDNAALQTSEGVNFIARFLGFFS
                     DGEYRYVDVLQPNHSDIIRYSGKDFPINQILNHIHLARYAVTFENNVDSKLRRH"
     gene            complement(857..1210)
                     /locus_tag="WGR74_001983"
     CDS             complement(857..1210)
                     /locus_tag="WGR74_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906324.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            1446..2873
                     /locus_tag="WGR74_001984"
     CDS             1446..2873
                     /locus_tag="WGR74_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ0906325.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQNRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVIAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            2942..4345
                     /locus_tag="WGR74_001985"
     CDS             2942..4345
                     /locus_tag="WGR74_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ0906326.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYEAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(4509..5102)
                     /locus_tag="WGR74_001986"
     CDS             complement(4509..5102)
                     /locus_tag="WGR74_001986"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEZ0906327.1"
                     /translation="MYGSHHLLCTQTDGDNAQKKTTTYKEQDPAKVTHYLTQPAEFSD
                     YQRVYLDETGFDRYLFRPYALSLKGQIVKAQISGKRYQRLSLVSAQVGNRLIAPMVYQ
                     NTMTGVFFEAWFQQCLLPALTQKSVIILDNARFHRMGVLREMAEKLGHKVLPLAPYSP
                     ELNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
BASE COUNT         1306 a         1261 c         1331 g         1259 t
ORIGIN      
        1 atttgatcaa tgaaagccga atctgataca ccgcgccgaa gaggaacgcc taacaggttt
       61 cctttaccgt ccgttatgta cgtttcgtaa cattcgaaga cttcctgaac cctgcctgcc
      121 gtttcggttt ctgtaccccc ccctgttaga taagggggga aggtttgaag cggttgccgg
      181 cttcctgccg tccgctggcg cgtccgtcat cacgccggca accgcctttg tcatcccttg
      241 cttatcttcc atggtgcgaa tcctcaaaaa cgggcaaaaa aaaagccctg ttacttgtag
      301 aaagtaaagg acgttaattt ttgttaatcg tcccttctta gggacgcaat atataaggga
      361 cgcaatatat aaggttttaa aacgctaaga acaaaatccg ataacggctc tgaagaagta
      421 agtctttctt tattgcacaa gatagcaaac ttccacggca ttctcgcccc acccgtgccg
      481 tccaagcctt tttcaaagac atagatggtt ttcttggcaa tcacttcgtc agtttggcgg
      541 tcgataatcc gtatggtcgc acctgccact cagtgcctgc gcagcttgga atcgacattg
      601 ttttcgaacg ttaccgcata acgggcgagg tgtatatgat taagtatttg atttatcgga
      661 aaatctttac ctgaataccg aataatatcg gaatggttgg gttgcaggac atccacatag
      721 cggtattccc catcgctaaa aaatcctagg aaacgagcaa taaaatttac tccttcgctg
      781 gtctgtaaag ccgcattgtc ccacatcggg tctctggttt ttgcatctgc cgaaacggta
      841 cgctcaggta ctaccttcaa cagcataatc ccttccacat tgtccgccgt ctggtaaatc
      901 tttcccccgc cgttttgcat tgttcgttaa acacggcttc ggcttctttg tattttctgt
      961 cccactcttc ttgtgcctgt atttcttctt tgatcgggcc gaattgtttg ggaataatcc
     1021 aaacaaacag catcaggata gcggcggcgg tcaggctgcc tgaaaggatt ttgccggggt
     1081 tccgtttggg ctttttatag gcaaagcgga cgagaaacca aagcaacagc agcatggtgc
     1141 cccaatagcc gattgagaat aggatggcca aaccttctag gaaatggcgt aaatcgtttg
     1201 tggtaaacat gggttgttcc tgtggttaaa tgtgcaggct gctttttgcc gaatcttgcc
     1261 gcatctcaaa agcagcctgc gcttcagcgt tgcgttacgc agtaaaataa tgaatatttg
     1321 taacggcttg ggtatttttt gtcaatattc ccgcctttcc cttaacagct gccgcgcttt
     1381 ccgttaaaat tcctttacat atttatattg tttcctgttt ctatattgcc aaggttatac
     1441 tcgttatgtt tttctccgcc ctgaaatcct ttctttcccg ctacattacc gtatggcgca
     1501 atgtttgggc ggtgcgcgac cagttggaac cgcccaaacg cacggcggaa gaacaggcgt
     1561 ttttgcccgc gcatttggaa ctgaccgata cgccggtctc tgccgctccg aaatgggcgg
     1621 cgcgttttat tatggcgttt gcgcttttgg ctttgttgtg gtcctggttc ggcaaaatcg
     1681 atattgtggc ggcggcttcg ggcaaaacgg tgtcgggcgg gcgcagcaaa accatccagc
     1741 cgctggaaac ggcggtggtt aaggcggtac atgtgcgcga cgggcagcat gtgaaacagg
     1801 gagaaacgct ggcggaactg gaggctgtgg gaacagacag cgatgtggtg cagtcggagc
     1861 aggctttgca ggctgcccaa ttgtccaaac tgcgttatga agcggtattg gcggcattgg
     1921 aaagccgtac cgtgccgcat atcgatatgg cgcaagcacg gtctttaggt ctctccgatg
     1981 ccgatgtgca atcggcgcag gtgttggcgc agcaccagta tcaggcatgg gcggcgcagg
     2041 atgcgcaatt gcagtcggct ttgcgcggcc atcaggcgga attgcagtcg gccaaggcgc
     2101 aggagcagaa gctggtttcg gtgggggcga tcgagcagca gaaaacagca gactaccgcc
     2161 gtttgcgggc cgacaatttt atttcggaac atgcgttttt ggagcagcag agcaaatcgg
     2221 tcagcaattg gaacgatttg gaaagtacgc gcggtcagat gaggcagatt caggcggcca
     2281 ttgcacaggc ggagcagaat cgggtgctga atacgcagaa cctgaaacgc gatacgctgg
     2341 atgcgctgcg ccaggcaaac gaacagattg accaataccg cggccaaacg gataaggcaa
     2401 agcagcggca gcagctgatg acaatacagt cgcctgcgga cggcacggtg caggaattgg
     2461 ccacctatac ggtgggcggt gtggtgcagg ctgcccaaaa aatgatggtg attgcgcccg
     2521 atgacgacaa aatggacgtg gaagttttgg tattgaacaa agacatcggt tttgtggaac
     2581 agggacagga tgcggtggtg aagattgaga gttttcccta tacgcgctac ggttatctga
     2641 cgggcaaggt gaaaagtgtc agccatgatg cggtaagcca cgaacagttg ggcttggttt
     2701 atacggcggt ggtgtcgctg gacaaacata ccttgaatat tgacggcaaa gcagtgaatc
     2761 tgacggcggg catgaatgtc acggcggaga ttaaaacggg taaacggcgg gtgctggatt
     2821 atctgttaag cccgctgcaa accaaattgg acgaaagctt tagggagcga taggcggatc
     2881 cgtactgggc atttgttatc cgccggttcg gacatgcaga ctgcctgaaa ccattgcccg
     2941 gatgacattg ctcaatctaa tgataatgca agattacggt atttccgttt gcctgacact
     3001 gacgccctat ttgcaacatg aactattttc ggctatgaaa tcctattttt ccaaatatat
     3061 cctacccgtt tcacttttta ccttgccact atccctttcc ccatccgttt cggcttttac
     3121 gctgcctgaa gcatggcggg cggcgcagca acattcggct gattttcaag cgtcccatta
     3181 ccagcgtgat gcagtgcgcg cacggcaaca acaagccaag gccgcattcc ttccccatgt
     3241 atccgccaat gccagctacc agcgccagcc gccatcgatt tcttccaccc gcgaaacaca
     3301 gggatggagc gtgcaggtgg gacaaacctt atttgacgct gccaaatttg cacaataccg
     3361 ccaaagcagg ttcgatacgc aggctgcaga acagcgtttc gatgcggcac gcgaagaatt
     3421 gctgttgaaa gttgccgaaa gttatttcaa cgttttactc agccgagaca ccgttgccgc
     3481 ccatgcggcg gaaaaagagg cttatgccca gcaggtaagg caggcgcagg ctttattcaa
     3541 taaaggtgct gccaccgcgc tggatattca cgaagccaaa gccggttacg acaatgccct
     3601 ggcccaagaa atcgccgtat tggctgagaa acaaacctat gaaaaccagt tgaacgacta
     3661 caccgacctg gatagcaaac aaatcgaggc catagatacc gccaacctgt tggcacgcta
     3721 tctgcccaag ctggaacgtt acagtctgga tgaatggcag cgcattgcct tatccaacaa
     3781 tcatgaatac cggatgcagc agcttgccct gcaaagcagc ggacaggcgc ttcgggcagc
     3841 acagaacagc cgctatccca ccgtttctgc ccatgtcggc tatcagaata acctctacac
     3901 ttcatctgcg cagaataatg actaccacta tcggggcaaa gggatgagcg tcggcgtaca
     3961 gttgaatttg ccgctttata ccggcggaga attgtcgggc aaaatccatg aagccgaagc
     4021 gcaatacgag gccgccgaag cacagctgac cgcaaccgag cggcacatca aactcgccgt
     4081 acgccaggct tataccgaaa gcggtgcggc gcgttaccaa atcatggcgc aagaacgggt
     4141 tttggaaagc agccgtttga aactgaaatc gaccgaaacc ggccaacaat acggcatccg
     4201 caaccggctg gaagtaatac gggcgcggca ggaagtcgcc caagcagaac agaaactggc
     4261 tcaagcacgg tataaattca tgctggctta tttgcgcttg gtgaaagaga gcgggttagg
     4321 gttggaaacg gtatttgcgg aataaagcag gctgaaacgg ttatgaaatt cccaaagcag
     4381 cctgcacccc gtttcgaaag tgcaggctgc tttgggattg atccgatact ttcacattct
     4441 cattgccttt aaataaagca cagttaaaaa cacatttaaa ttaagtgcga attaattcaa
     4501 atgtatattc aattaaaatc aaaataggac agtagtgcat cgtcaaatcg ggcgtaatca
     4561 gacaaaacgg ttcgcagata ccgcttaata ttcgcccaca ctttctcaat cgggttaagc
     4621 tcaggtgaat aaggtgcaag aggcaatacc ttatgtccca atttttccgc catttcccgt
     4681 aagacaccca tacggtgaaa tcgcgcatta tctaaaataa tcaccgattt ttgagtcaat
     4741 gcgggcagta ggcattgctg aaaccacgct tcaaaaaaga ctccggtcat cgtattttga
     4801 taaaccatcg gagcaatcag ccggttgccg acttgtgcgg acaccaaaga taagcgttgg
     4861 tatctttttc cacttatctg cgctttcact atttgccctt tcaggctgag ggcataggga
     4921 cggaacaggt agcggtcaaa tcctgtttca tccaaataaa cacgttggta gtcggaaaat
     4981 tcggccggct gtgtcaaata atgcgttact ttggccgggt cttgttcttt gtaagtggtg
     5041 gtcttttttt gcgcgttatc cccatctgtt tgagtgcata gcaaatggtg gctgccgtac
     5101 aatcaaaatg tttggcgatt tcatgcagat aggcatccgg gtgttgccca acatatt
//
LOCUS       DAWFLT010000113         4945 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWFLT010000113 DAWFLT010000000
VERSION     DAWFLT010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.113"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            129..350
                     /locus_tag="WGR74_001987"
                     /pseudo
     CDS             129..350
                     /locus_tag="WGR74_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(332..640)
                     /locus_tag="WGR74_001988"
     CDS             complement(332..640)
                     /locus_tag="WGR74_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906328.1"
                     /translation="MTPWADAYADLRGKTKVMTTQMCASRDVSKSAKGWSVGIGLNVG
                     KQLTDSVGLEFDPYYRHKTICKPREIVLDGDKTKMGRSKSNEYGFRVTATFYSQLKSK
                     "
     gene            867..1094
                     /locus_tag="WGR74_001989"
     CDS             867..1094
                     /locus_tag="WGR74_001989"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906329.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCSVPD
                     CRGNGKTDVPCGFVRMNGGYCFYQSVSFSLE"
     gene            1256..2311
                     /gene="aroG"
                     /locus_tag="WGR74_001990"
     CDS             1256..2311
                     /gene="aroG"
                     /locus_tag="WGR74_001990"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ0906330.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWDATEE
                     LLALLAGANKKRMARAG"
     gene            2379..2867
                     /locus_tag="WGR74_001991"
     CDS             2379..2867
                     /locus_tag="WGR74_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ0906331.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAGRTERRVSSKGTSYAFVHYL
                     RY"
     gene            complement(3217..3693)
                     /locus_tag="WGR74_001992"
     CDS             complement(3217..3693)
                     /locus_tag="WGR74_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ0906332.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GKRAWLTLKGYISEISRSEFEYEIPLADAEKIMETMCPFKMEKRRYPVRWNGSLFEVD
                     VFLGENAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSKHPFSSWKNAV"
     gene            complement(3705..4133)
                     /locus_tag="WGR74_001993"
     CDS             complement(3705..4133)
                     /locus_tag="WGR74_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ0906333.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQNYSNTFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            4245..4490
                     /locus_tag="WGR74_001994"
     CDS             4245..4490
                     /locus_tag="WGR74_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906334.1"
                     /translation="MLKSIELNSHIRNRLAAYLKGRGMDFQTAMREEKGNKEIASIVH
                     SGLPTLVRKLYSEQKMQKFVWEKRDLIADYISRRMQG"
BASE COUNT         1301 a         1154 c         1259 g         1231 t
ORIGIN      
        1 aaaaaccagt acggcgttgc ctcgccttag ctcaaagaga acgattctct aaggtgctga
       61 agcaccaagt gaatcggttc cgtactattt gtactatctg cggcttcgtc gccttgtcct
      121 gatttttgtt aatccgctat attttagata atgcgcgatt tcaccgtatg ggtgtcttac
      181 gggaaatggc ggaaaaattg ggacataagg tattgcctct tgcaccttat tcacctgagc
      241 tcaacccgat tgagaaagtg tgggcgaata ttaagcggta tctgcgaacc gttttgtctg
      301 attacgcccg atttgacgat gcactactgt cctactttga ttttaattga ctatagaacg
      361 ttgcggttac gcggaagccg tactcgttgg atttggagcg gcccattttg gttttgtcgc
      421 cgtccaaaac aatctcacgg ggtttgcaga ttgttttgtg acggtagtat ggatcaaact
      481 cgagaccgac gctgtcggtc aactgtttgc ctacattcag accgataccg acactccaac
      541 ctttggcgct tttgctgaca tcgcgggaag cacacatctg ggtcgtcatc actttggttt
      601 tgccgcgcaa atctgcatat gcatccgccc aaggggtcag ggatcatccg tcccccaaat
      661 cttggcggat ttcgccatag actttcaaaa caaggttttc atgcttggta acggtgtttt
      721 tccttatcgc cgatgatggc tttgcccttg ccgttagact caggaatatc ggctaccgta
      781 acggcggaca cggctgcaag tgagagtgca agcagggttt tttcatgttt ttcttcctat
      841 aatgaggatg aataaatgga gaaagtgtgg gaaatacccg cattccaatt aaatcttttt
      901 tcaagcaatt agttcttttt gttttcaaca ttttccttgc gtatgaaatt ttgccggatg
      961 tgcaggcgga atcggcagcg gggtgttctg taccggattg tcgtggaaat gggaaaacgg
     1021 atgttccgtg cggttttgtc cgaatgaatg gcgggtattg tttttatcaa tctgtttctt
     1081 tttctttgga ataaaattcc taaaatatta aaaatatgaa atttaaaatc tatgaaaaaa
     1141 gatatatcag ttattttgaa ataaaatagc tttgtagtaa tatgttgcac ttgtttgtgc
     1201 aaggtaaacg atgtaaccta agccgcgtat aaaaaaccat caggaaagat gcaagatgac
     1261 acaccattac cccacagacg atattaagat taaagaagtt aaagagttgt tgccgccgat
     1321 tgcccatctt tacgagctgc cgatttccaa agaggcttcg ggcttggttc accgcacccg
     1381 tcaggaaatt tccgatttgg ttcacggcag ggacaagcgg ctgttggtta ttatcgggcc
     1441 gtgttcgatt cacgatccga aagcggcgtt ggaatatgcg gagcgtttgt tgaaactccg
     1501 caagcagtat gaaaacgagc ttttgattgt gatgcgcgtt tatttcgaga agccgaggac
     1561 gacggtgggt tggaaaggtt tgattaacga cccgcatttg gacggtacgt ttgacatcaa
     1621 tttcggtttg cgtcaggcgc gcagcctgtt gttgtcgctg aacaatatgg gtatgcctgc
     1681 ctctaccgag tttttggata tgattacgcc gcaatattat gcggacttga tttcttgggg
     1741 ggcaatcggt gcgcggacga ccgaaagcca agttcaccgc gaattggcaa gcgggctgtc
     1801 ctgccccgtc ggctttaaaa acggtacgga cggcaatttg aagattgcca tcgacgcaat
     1861 cggtgcggcg agccattcgc atcatttttt gtctgtaacc aaggccgggc attccgccat
     1921 tgtccatacc ggcggcaatc ccgactgtca tgtcattttg cgcggcggca aagagccgaa
     1981 ttatgatgcg ggacacgtca gcgaggcggc ggaacaactg cgtgcggcag gcgtaaccga
     2041 caagctgatg atagattgca gccacgccaa cagccgcaag gattacactc ggcagatgga
     2101 agtggcacaa gatattgccg cccaattgga acaggacggc ggcaatatca tgggcgtgat
     2161 ggtggaaagc catttggtcg aaggcaggca ggacaagccg gaagtgtacg gaaaaagcat
     2221 taccgatgcg tgtatcggtt gggacgcgac tgaagaactg ttggcattgt tggcaggtgc
     2281 aaacaaaaaa cgtatggcgc gcgccggttg agatttttga cacggaatgt cataaaatgc
     2341 cgtctgaagc gttcagacgg catttttgtg gaggaaatat gctcaaaata accctaattg
     2401 cggcgtgtgc ggaaaacctg tgcatcgggg cgggcaatgc tatgccttgg cacatccccg
     2461 aagatttcgc atttttcaaa gcctatacct tgggcaaacc cgtcattatg gggcggaaaa
     2521 cgtgggaatc cctgcccgtc aaacccctgc ccggacggag gaacatcgtc atcagccggc
     2581 aggcggatta ttgcgcggca ggcgcggaaa cggcggcaag tttggaggcg gcattggcat
     2641 tgtgcgcagg cgcggaagaa gccgtcatta tgggcggcgc gcagatatac ggacaagcga
     2701 tgccattggc gaccgatttg cggataaccg aagtggattt gtctgtggaa ggagatgcat
     2761 ttttccccga aatagaccgg acgcattgga aagaggcagg gcggacggaa cgccgcgtca
     2821 gcagcaaagg cacgagctat gcttttgtgc attatttgag atattgaaat ataaactctc
     2881 tataaaatcc cccgcaaatg atgggctgaa atagaaaata ttgttattcc cccgaagatg
     2941 ggaatccggg attttaaagt tagggtaatt tatccgaaat aacaacaatc ttccatcgtc
     3001 attcccgcaa aagcgggaat ccggaaacga aaagctaaag caatttatcg gaaaaaaccg
     3061 aagtttaaag aaccggattc ccgcctgcgc gggaatgacg agattttagg ttatggggat
     3121 ttattgggaa taatggaaca aagaaagcag aaataaggat atagaggctg tctttggatt
     3181 tgcgatggtt gtcggagaat gccgtctgaa gccgcttcag acggcatttt tccagcttga
     3241 gaacggatgt ttgctcaaat aagcattggt aaacataccg tcggcagtga tttcgcgccc
     3301 cagccagtcc ggacggtcaa aatcggcatt ttcgtcgggc agctcgattt ctgcgacgac
     3361 caaaggtgcg ttttctccaa ggaaaacatc gacttcaaac aggctgccgt tccatctgac
     3421 cggataacgc cgtttttcca tcttaaacgg gcacatcgtt tccattatct tttccgcatc
     3481 ggcaagcggg atttcgtatt caaactcact gcggctgatt tccgaaatat agcctttcag
     3541 cgtcagccac gcccgttttc cggcaatgcg gacacggacg gtgcgttctt tttcaacaga
     3601 cagataaccc tgcctcaaca gcagcggctc atcggcgtat tgccgccagt tgtcgtttcc
     3661 aatcaaaaaa cggcgttcga tttctatcgg cataagatgc tccgtcaaaa cggtttgaac
     3721 acgaccagat acagcgcggc aaccatcagc agcacgggga tttcgttaaa tacgcggtac
     3781 cagcggtgtg agaaagtatt gctgtaattc tgaaaacggc gcagcagcac gccgcaatac
     3841 aactggtaag ccaaaagcat caagcccaaa cacagtttga cgtgtaccca gccgctgccc
     3901 caccagccgg cggcaaacgg tatcgccgcg ccgaacacga ccgcaccgaa acctaaaggg
     3961 gacataaaac ggtacaaccg caccgccata cccgacaggc gcacatactc gggattgccg
     4021 cgcggcacat caatcatcgc catattgacg aaaatcctcg gcaggtaaaa cagccctgca
     4081 aaccacgaaa tgacaaaaaa caagtgaaac agcttgaacc aagaaaacat catcgcccac
     4141 accctgccga aaagcggtat tgtacaggca aaccgcttgg gaaacgtgat aaaatcaggc
     4201 ggataaacaa atcgaataaa tccttaccgc aaaacggagg caaaatgctc aaatccatcg
     4261 aactcaattc ccacatccgc aaccgccttg cagcatatct gaaaggcagg ggtatggatt
     4321 ttcagacggc gatgcgggag gaaaaaggca acaaagaaat cgcctccatc gtccacagcg
     4381 gtctgcccac tctggtccgc aaactgtatt ccgaacaaaa aatgcagaag tttgtttggg
     4441 aaaagcggga tttgattgcc gactacatca gccgccggat gcagggatag gcggctgaaa
     4501 tctgttttca ggcaagtgaa aagacaatat ggcagattga aattatgctt atcgtcattc
     4561 ccgcccgcgc gggaatccga cttgtttggt ttcggttatt tttcgtttcg taacttttga
     4621 gccgtcattc ccgcgcaggc ggtaatccgg cttgttcggt ttcggttctt tttctcgttt
     4681 cgggtgattt ctaaaccgtc attcccgcgc aggcgggaat ctaggtcttt aaacttcggt
     4741 tttttccgat aaatttttgc cgcattaaaa ttctagattc ccgctttcgc gggaatgacg
     4801 gcggagggtt tttagttttc ccgaaaatgc acatcatcca aaatcccgtt attcccacaa
     4861 aacagaaaat caaaaacaac aatctgaaat tccgtccttc ccgcccgcgc gggaatccga
     4921 cttgtccggt ttcggttctt tttct
//
LOCUS       DAWFLT010000114         4875 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWFLT010000114 DAWFLT010000000
VERSION     DAWFLT010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.114"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(440..820)
                     /locus_tag="WGR74_001995"
     CDS             complement(440..820)
                     /locus_tag="WGR74_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ0906335.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMPPVQNQAGTADF
                     RAFSCENGLSVHVRRLDGGRIALRLDGRRAVLSSDVAASGERYTAEHGLFGNGTEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            1292..3373
                     /locus_tag="WGR74_001996"
     CDS             1292..3373
                     /locus_tag="WGR74_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ0906336.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            3363..3557
                     /locus_tag="WGR74_001997"
     CDS             3363..3557
                     /locus_tag="WGR74_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ0906337.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(3753..3944)
                     /locus_tag="WGR74_001998"
     CDS             complement(3753..3944)
                     /locus_tag="WGR74_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906338.1"
                     /translation="MDFQFFVETIRRQEKKLKHGVYRLFEIYSWLEDGKATKARIRYF
                     VKIEVDKQILRRIHRGIAD"
     gene            4073..4633
                     /locus_tag="WGR74_001999"
     CDS             4073..4633
                     /locus_tag="WGR74_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HEZ0906339.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINLPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT         1162 a         1383 c         1198 g         1132 t
ORIGIN      
        1 ttgaaagcgg ccaacaggtt aagtgcgccc gctgccgcct aaaaagcagc ccggatgcct
       61 gattatcggg tatccgggca ggattaaggg ggtgtttggg taaaattagg cggtatttgg
      121 ggcgaaaaca gccgaaaacc tgtgtttggg tttcggctgt cgggagggaa aggaattttg
      181 caaaggtctc aggccgtctg aaaaacagct tcgacaaaga cgcggttgcc aaagagatcg
      241 acctacaaag ggaagtaaca caggcgttcg gcaaaacgtc gcccaagtcg cagcggccgt
      301 tgccaacaaa cttggcaata cccgaagtta cggacggtat caggtagcca aaaccctgcc
      361 ggaggccgaa ctgcaaaaca aatccgacat aaaaaaccgc ccgaaaaatc cggacggcgg
      421 ttcaaacagg ctgccccgtt tagcgggcgc ggcaggaggt ttcgaccgaa ttgccgtagg
      481 catcggtaaa gccgaaaaag gcttcgccgc ctttctgatg ccactcggtt ccgtttccga
      541 acaaaccgtg ttcggcggta tagcgttcgc cggatgcggc aacgtcggaa gagaggacgg
      601 cacgcctgcc gtccaaccgc aacgcgattc tgccgccgtc caaacggcgg acgtgcacag
      661 acaaaccgtt ctcgcaggaa aatgcccgaa aatctgccgt gccggcttgg ttttgaacgg
      721 gcggcatatg ccctcgtccg ccgtcatcat acgcctccgg cacggcacag gccgccaaag
      781 acaaaaccgg tacggtcagc gcgaaaaacc tgatattcat aaaagctccc caataaaaat
      841 aagatatgaa acaaccgccc tgattccaag ctgcggcaac gccatactat aaacggacgc
      901 gcaaacacac aagcccgata accggaattt acccgcgatg aatcaataat ccggattgcg
      961 cgcccctttc ccgtattccg gcaccgcctt ttgcctgacg atgccgtctg aacctccctt
     1021 cccgtcagga ggaatgtaaa ttttttccaa attccaagta aaaaccgcta tcagtgtgct
     1081 aatttgcgtt aaaatcctat tcggcgttta acgttttgtg cgcccgcatc cctgcactgt
     1141 ttgatgcggg cataaggcac aaatcccgac aagcgcactg tttcatactt cgtcaatcat
     1201 tcagactccg gtttgtgccc gtgccggcag gcggtcatcc gtttcccgcc gttcaggcat
     1261 attccgacag tgtgagataa ggatttattc gatgaaatca ctcaaaacct tcctcatttg
     1321 gggcatagtg gtactggtcg gcttagcatc ctttaccact ctggctctca gccgaggcga
     1381 acaggtcagc gcggtatgga tggttaccgc cgccatatcc gtttactgca tcgcctaccg
     1441 tttttacagc ctctacatcg ccaaccgcgt aatgcggctc gatcctaacc gcctgactcc
     1501 ggcagaacgc cacaacgacg gcttggacta cgttccgacg cacaagggcg tattgttcgg
     1561 acaccacttt gccgcgattg ccggcgcggg ccctttggtc ggccctgtac tggctgcgca
     1621 aatgggttac ctgcccggta ctttgtggat tatcttcggc gtggtttttg ccggcgcggt
     1681 acaggatatg atggtactgt ttgtttctat gcgccgtgac ggtaagtctt tgggcgatat
     1741 tgtgaaacag gaactcggca ctgtccccgg cgtgattgcc tccatcggta ttttgatgat
     1801 tatggtcatc attatggcgg tgttggcgtt gattgtcgta aaagcacttg ttcacagccc
     1861 ttggggtacg tttaccatcg ccgccactat gccgattgcc ctgtttatgg gtatttacac
     1921 gcgctatatc cgtccgggca aaatcggcga gatttccatc gtcggcttta ttttgctgat
     1981 gctggcggta atttacggcg aagatgtggc gaaaagttcc atcggacatt ggttcgacct
     2041 tgacggcatc cagctcactt gggcgattat gatttacggc tttgtcgcct ccgtattgcc
     2101 cgtatggttg ctgctcactc cgcgcgacta tctctccacc ttcctgaaaa tcggtacgat
     2161 tgcggccttg gctttgggca tcgtcatcgt caatcccact ttgcaaatgc ctgccgtaac
     2221 ccactttatc gacggttcgg gtccggtatt ctcaggcgca ttgttcccgt tcctcttcat
     2281 caccatcgcc tgcggtgcgg tttcgggctt ccacgcgctg atttcctccg gcaccacgcc
     2341 gaaaatgctg gaaaacgaaa cccacgtccg tatgattggt tacggcggta tgttgatgga
     2401 aagtttcgta gccattatgg cgcttgccgc cgccgcatcg cttgatcccg gcgtgtactt
     2461 cgccatgaac agccctgccg ccctgatcgg tacggatgcc aacaccgccg ccgaagtgat
     2521 taccaccaag ctgcaattcc ctgtcgatgc ggcaaccctg ttgcataccg ctaaggaagt
     2581 gggcgaaaac accatccttt cccgtgcagg cggtgcgcca accctcgcag tcggtatggc
     2641 gcacattatg agccgcctga ttccgggcga ggcgatgatg gcgttctggt atcacttcgc
     2701 cctattgttt gaagccttgt tcatcctgac cgccgtcgat gccggtacgc gcgtcgcacg
     2761 ttttatgatt caagacttgg gcagcatctt ctacaaacct ttcggcaaca ccgactccat
     2821 ccccgccaac ctgattgcga ccttcttcgc cgtggcattg tggggctact tcctctacac
     2881 cggcgtaacc gacccgctgg gcggcatcaa ctcgctctgg cctttgttcg gcatcgccaa
     2941 ccaaatgctg gcaggcgtgg ccttgattat gtgcgccgtg gtgctgatta agatgaaacg
     3001 cgaccgttat gtttgggtgg tactcgttcc cgccgtcggc gtactgttcg taacctgcta
     3061 cgccggcctg caaaaactgt tccacagcga cccgcgcatc agcttccttg cccacgccgg
     3121 caaatacagc gacgcattgg ctaaaaacga agtccttgcg cctgccaaag acatcggcga
     3181 aatggcgcaa atcatcttca acgacaagat taatgccggt ctgaccatcc tcttcttgtc
     3241 ggttgtcgtg attgtcgccg cgtacggttt gcgtaccgcc ctcaaagcac gcaaagtcgg
     3301 ctggccgacc gccaaagaaa tcccggcggt gtaccgcgac ggcaagcagc cggaggcaca
     3361 aagtgaagca taagctcgcg tcttggtgga aaaccatcaa gctgacggca aacttgatgg
     3421 caggcgtgcc tgattatgaa aactacgttg cacagcagcg caaacataat cccaacgccc
     3481 ccgtgatgac caagctgcag tttcaagact attgccgcaa acgccgctgc ggcgcaaacg
     3541 gcggacgctg ctgttagccc cgcctgaaac aaatgccgtc tgaacgccgc ttcagacgga
     3601 atttttataa tatagtggat taacaaaaat caggacaagg cgacgaagcc acagacagta
     3661 caaatagtac gaaaccgatt cacttggtgc ttcagcacct tagagaatcg ttctctttga
     3721 gctaaggcga ggcaacgacg taccggtttt tgttaatccg ctataccacg atgaatcctt
     3781 cgcaatatct gtttatcgac ctcaattttg acaaaatacc ggatacgcgc ctttgttgct
     3841 tttccatctt ccaaccaact gtaaatctca aacagccggt acacgccatg cttcagtttc
     3901 ttttcctgtc ggcggattgt ttcgacaaag aattgaaaat ccatttcatg caccttaaaa
     3961 tttaatctgc attcaaacct tttcactttg gaagcaccat ttatcggatg tcccttcgca
     4021 ataaacaaat tttcccgata ccgccgccca tttcaaccca aacccaaaac ctatgaaaaa
     4081 cctcatcgcc ttcaacaaac cctatggcgt tatctgccaa ttttcaccgc acgaaaaaca
     4141 caaaagcctc aaagacttta tcaatcttcc cggcttctac cccgccggac ggctcgacac
     4201 cgacagcgag gggctgctgc tgctgaccga cgacggcagg cttcaggcac aaattaccga
     4261 ccccaaattc aaacacccta aaacctactg ggcgcaactg gagggcgtac ccgacgaaag
     4321 ccgattggaa agcctaagaa aagggataga cttaggcggt ttcgttaccc gtccggcaag
     4381 catccgcatc ttgaaacacg gagaagcaga ttcgttatgg gagcgcatcc cgccgatacg
     4441 cgtccgcaaa accgttcccg atttttggat tgaaattacc atttctgagg gcaaaaaccg
     4501 ccaagtcagg cgaatgaccg ccaaggcggg ctatccctgc ctgcgtctga tcagagtggc
     4561 aagcggcagg ctgaaactgt ttgatttgga tttaaaaccc ggggaatggg catacgcccc
     4621 gtttaaacca taatcacgtt tatctcatca tttccacaaa agtgggaatc cggaatttta
     4681 tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacga
     4741 aaccgattca cttggtgctt cagcacctta gagaatcgtt ctctttgagc caaggcgaga
     4801 caacgccgta ctggtttttg ttaatccgct atattccgcc atctctaaga tttacagcga
     4861 tacacgggtg attta
//
LOCUS       DAWFLT010000115         4803 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWFLT010000115 DAWFLT010000000
VERSION     DAWFLT010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.115"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            227..1069
                     /locus_tag="WGR74_002000"
     CDS             227..1069
                     /locus_tag="WGR74_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ0906340.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDETWAQLAVDELVMFREGKIILNDRPRHPVYMSVEEGLETARAA
                     GVAV"
     gene            1099..1563
                     /gene="nrdR"
                     /locus_tag="WGR74_002001"
     CDS             1099..1563
                     /gene="nrdR"
                     /locus_tag="WGR74_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ0906341.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPNCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSAALADMVLKELLEHNTEAAVRFAALHKRFDNPADFASWLAQAVKTGGKA"
     gene            1595..2704
                     /gene="ribD"
                     /locus_tag="WGR74_002002"
     CDS             1595..2704
                     /gene="ribD"
                     /locus_tag="WGR74_002002"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ0906342.1"
                     /translation="MFSDTDISMMENALRLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLAMLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITSEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERDEDKLRPYRKHAHVRILMPSETAGGK
                     IDLNSLMRLLADEGIGELIVEAGSELTSAFLAEDLADEIVLYRSPKILGSGKDLFSLL
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNGNAF"
     gene            2899..4320
                     /locus_tag="WGR74_002003"
     CDS             2899..4320
                     /locus_tag="WGR74_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ0906343.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVSKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEANAPPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACAASFW
                     LFFVFKTESSCRLWQPLKRLPLYMHTLFCLASSAAYTCFGTPANYPLFAGIWAAYLAG
                     CILRHRKDLHKLFHYLKKQGFPL"
     gene            4317..>4803
                     /locus_tag="WGR74_002004"
     CDS             4317..>4803
                     /locus_tag="WGR74_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0906344.1"
                     /translation="MKIVLTTSMAGLGGTETATVRLGRLLRQHGHDIMLASSDGPLLK
                     EAQALGIRWQPVDFYRGGLFGYLKGMIAYARMLRREQPDIIDCQMARVVPACAIAAKI
                     VSPKTKIIYHSHGLDAATYPKIAKLFDKLGVYIIGNCKHEREKLIRHGFPADRIVYAY
                     NA"
BASE COUNT          982 a         1619 c         1228 g          974 t
ORIGIN      
        1 cgcctgcgcg ggaatgacgg ctcaaaagtt acggaacgaa aaacaaccaa aaccggacaa
       61 gtcggattcc cgcctgcgcg ggaatgacgg aatcttaagt ttccgtcttt gttttctgtt
      121 ttctgttttc gagggaataa cggggaacaa gccgtatttc agacggcatt ttcagttcgg
      181 ggtataatcc gaatacttgc gaccatctga atcattggga caaaccatgt gtcaactgct
      241 gggcatgaac tgcaatacgc cgaccgatat tatgttttcc tttgaaggct tccgccgcag
      301 gggcggcatt accgaccatc acgccgacgg tttcggcatc ggctttttcg aaggcaaagg
      361 cgtgcgcctg ttccatgacg acaaacccag tgccaattcg cccgtcgccg acctcgtgcg
      421 cgcctaccag attaaatcgg aaaacgtcgt cgcccatatc cgcaaagcat cgcaaggaca
      481 aacctcgctg gcgaacaccc atccctttat gcgcgaaatg tggggcggct actggctgtt
      541 tgcccacaac ggacatttga ttgatttttt ccccgaacag ggcgaatttt tccaccccgt
      601 cggcacgacc gattccgaac gcgcgttctg ccacatcctc aaccgcctgc gcacccgctt
      661 tgccgcccgt cccgacgacg acacgctgtt tgacgcgatt gcggggctga cgcacgaaat
      721 ccgcaagttc gggctgttta actttatgct ttcagacggc attgccctgt ttgcccacgc
      781 cagcacgctg ctgcactaca tcgtccgcca agccccgttc ggcaaggcgc gcctgctcga
      841 cgacgacgtg atggtcgatt ttgccgaagt aaccacgccg tccgaccgcg tcgccgttat
      901 cgccaccctg ccgctgacgc gcgatgaaac gtgggcgcaa cttgccgttg acgaactggt
      961 gatgttccgc gaaggcaaaa ttattttgaa cgaccgaccc cggcaccccg tttatatgag
     1021 tgtcgaagaa ggtttggaaa ccgcgcgcgc cgccggcgtc gccgtctgaa cttcagacga
     1081 cataggagga cgaacccgat gaaatgccca ttttgcgccc accccgacac ccgcgttgcc
     1141 gattcgcgtc tgatggaaga acgcaacgcc gtgcgccgcc gccgccactg ccccaactgc
     1201 ggcaaacgct tcggcacgct cgaaaccgcc gaactcaaaa tgcccgccgt catcggtccg
     1261 gacaaaaaac gctcgccctt taatgcacaa cgcctccgca acgacctgac cgccgccgcc
     1321 cgaaaatccg ccctgacacc cgaacagatc gacgaaaccg tccgcctgac ggaacacagg
     1381 ctctacactt cgggtcagcg cgacatcccc tctgccgcac ttgccgacat ggtgctaaaa
     1441 gagctgcttg agcacaacac ggaagccgcc gtccgtttcg ccgccctgca caaacgcttc
     1501 gacaatccgg cagactttgc ctcgtggctg gcgcaagccg tcaaaacagg cggcaaagcc
     1561 tgattccccc aacccatact gatacggtat ccctatgttt tcggacacag atatatccat
     1621 gatggaaaac gccctccgac ttgccgcttt ggggcgtttt tccacttcgc ccaatccgcg
     1681 cgtcggctgc gttatcgcac acggcagcca aattgtcggg caaggcttcc acgtcaaagc
     1741 gggcgaaccc catgccgaag tccacgccct gcgtcaggcg ggcgaaatgg cacaaggcgc
     1801 gaccgccttt gttaccctcg aaccgtgcag ccattacggg cgtaccccgc cctgcgccga
     1861 agctctgctc cgttccggcg taacacgcgt cgttgccgcc atgcgcgacc ccaacccgct
     1921 ggttgccggc aaagggcttg ccatgctcga agcagcaggc atcaagacgg aatgcggttt
     1981 actcgaacat caggcaaggg aactcaaccg aggcttcctg tcgcgcatcg aacgccgccg
     2041 ccccttcgtc cgcctcaaat gcgccgtttc gctggacggc aaaaccgccc tttcagacgg
     2101 cagcagcttt tggattacca gcgaagacgc gcgtgccgac gtacaggttt tgcgtgccga
     2161 aagctgcgcg gtgctgaccg gcatcggcac ggtgttggcg gacaatcccc ggctcaacgt
     2221 ccgcgctttt ccaactttgc gccaacccgc acgcatcgtt ttagacagcc gcctgcgcct
     2281 gcccttggac agccaccttc tgaccgacgg acaatctcca acctacatcg ccaccttgga
     2341 acgcgatgaa gacaaactcc gcccctatcg caaacacgcc cacgtccgca tcctgatgcc
     2401 gtctgaaacg gcaggcggca aaatcgattt gaacagcctg atgcgcctgc ttgccgacga
     2461 aggcataggg gaactcatag tcgaagcagg ctccgaactc acatctgcat ttttggcaga
     2521 agatttggca gacgaaatcg tgctgtaccg ttcgcccaaa atcctcggca gcggcaaaga
     2581 cctgttttcc ctgctcgaaa accgcgccgc cctttccgca ccgcccttgt ggacacccgt
     2641 ttcaagcgaa atcctcggac acgacatcaa aaccgtgttc cgaaaaaacg gcaacgcctt
     2701 ttaaagggtt tgcgccgttt cactatataa taacgccgat aaaaaacggc accattcaga
     2761 ccgccgccca cccgaaaaaa cgcaacccgg actgccgcac cccgccggca gccgcgacgg
     2821 tctgaaagcc gtcaaattcc gatcaagaaa ggcttcagac ggcacaggca gcatcccgcc
     2881 gccgcccgga catcaaaaat ggacacaaaa gaaatcctcg gctacgcggc aggctcgatc
     2941 ggcagcgcgg ttttagccgt catcatcctg ccgctgctgt cgtggtattt ccccgccgac
     3001 gacatcgggc gcatcgtgct gatgcagacg gcggcgggac tgacggtgtc ggtattgtgc
     3061 ctcgggctgg atcaggcata cgtccgcgaa tactatgccg ccgccgacaa agacactttg
     3121 ttcaaaaccc tgttcctgcc gccgctgctg tctgccgccg cgatagccgc cctgctgctt
     3181 tcccgcccat ccctgccgtc tgaaatcctg ttttcgctcg acgatgccgc cgccggcatc
     3241 gggctggtgc tgtttgaact gagcttcctg cccatccgct ttctcttact ggttttgcgt
     3301 atggaagggc gcgcccttgc cttttcgtcc gcgcaactcg tgtccaagct cgccatcctg
     3361 ctgctgctgc cgctgacggt cgggctgctg cactttccgg cgaacaccgc cgtcctgacc
     3421 gccgtttacg cgctggcaaa ccttgccgcc gccgcctttt tgctgtttca aaaccgatgc
     3481 cgtctgaagg ccgtccggcg cgcaccgttt tcatccgccg tcctgcatcg cggcctgcgc
     3541 tacggcatac cgatcgcact aagcagcatc gcctattggg ggctggcatc cgccgaccgt
     3601 ttgttcctga aaaaatatgc cggcctagaa cagctcggcg tttattcgat gggtatttcg
     3661 ttcggcggag cggcattatt gttccaaagc atcttttcaa cggtctggac accgtatatt
     3721 ttccgcgcaa tcgaagcaaa cgccccgccc gcccgcctct cggcaacggc agaatccgcc
     3781 gccgccctgc ttgcctccgc cctctgcctg accggcattt tctcgcccct cgcctccctc
     3841 ctgctgccgg aaaactacgc cgccgtccgg tttatcgtcg tatcgtgtat gctgccgccg
     3901 ctgttttgca cgctggtaga aatcagcggc atcggtttga acgtcgtccg caaaacgcgc
     3961 ccgatcgcgc tcgccacctt gggcgcgctg gcggcaaacc tgctgctgct ggggcttgcc
     4021 gtaccgtccg gcggcgcgcg cggcgcggcg gttgcctgtg ccgcctcatt ttggctgttt
     4081 tttgttttca agaccgaaag ctcctgccgc ctgtggcagc cgctcaaacg cctgccgctt
     4141 tatatgcaca cattgttctg cctggcctcc tcggcggcct acacctgctt cggcacgccg
     4201 gcaaactacc ccctgtttgc cggcatatgg gcggcatatc tggcaggctg catcctgcgc
     4261 caccggaaag atttgcacaa actgtttcat tatttgaaaa aacaaggttt cccattatga
     4321 aaatcgtttt gaccacatct atggcaggct tgggcggcac ggaaaccgcc accgtccgcc
     4381 tcggcaggct actcaggcag cacggacacg acatcatgct tgcctcttcg gacggaccgt
     4441 tgctcaaaga ggcgcaagcc ttgggcatac gttggcaacc cgtcgatttc tatcgcggcg
     4501 gccttttcgg ctacctcaaa ggtatgattg cctacgcccg aatgctgcgg cgcgaacaac
     4561 ccgacatcat cgactgccaa atggcgcgcg tcgtacccgc ctgcgccatt gccgccaaaa
     4621 tcgtttcgcc gaaaaccaaa atcatctacc actcgcacgg tttggacgcg gcaacctacc
     4681 ccaaaatcgc caaactcttc gacaagctgg gcgtgtacat catcggtaac tgcaaacacg
     4741 aacgcgaaaa actcatccgc cacggtttcc ccgccgaccg gattgtctac gcctacaacg
     4801 cac
//
LOCUS       DAWFLT010000116         4769 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWFLT010000116 DAWFLT010000000
VERSION     DAWFLT010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.116"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..184
                     /locus_tag="WGR74_002005"
     CDS             <1..184
                     /locus_tag="WGR74_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906345.1"
                     /translation="DKLDVSGFIHYRINHSKEFADRQNHINGIENFWNQAKRVLRKYS
                     GLTKIRTRRRSRRQYR"
     gene            282..398
                     /locus_tag="WGR74_002006"
                     /pseudo
     CDS             282..398
                     /locus_tag="WGR74_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(431..1540)
                     /gene="mltB"
                     /locus_tag="WGR74_002007"
     CDS             complement(431..1540)
                     /gene="mltB"
                     /locus_tag="WGR74_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ0906346.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(1537..1719)
                     /locus_tag="WGR74_002008"
     CDS             complement(1537..1719)
                     /locus_tag="WGR74_002008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906347.1"
                     /translation="MPSERGSAFRRHFRRRQADFGRRKRQRAAFCKAADCGGYRISRR
                     ALAPLSHLPYLFGTYL"
     gene            complement(1741..3168)
                     /locus_tag="WGR74_002009"
     CDS             complement(1741..3168)
                     /locus_tag="WGR74_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0906348.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDAAACLLNDIRKDILDCRYCG"
     gene            3353..>4769
                     /locus_tag="WGR74_002010"
     CDS             3353..>4769
                     /locus_tag="WGR74_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ0906349.1"
                     /translation="MTYPIPKPREKSRWSNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKEHILPDLAVNRQSDEPLQALKDFQTTFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAA"
BASE COUNT         1023 a         1332 c         1299 g         1115 t
ORIGIN      
        1 cgacaagttg gacgtgagcg gttttatcca ttaccgcatc aaccattcca aggagtttgc
       61 agaccgtcag aaccacatta acggcattga gaacttttgg aatcaggcaa aacgcgtctt
      121 gcgaaaatat agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagtacag
      181 atagtacgga accgatttac ttggtgcttc agcaccttag agaatcgttc tctttgagct
      241 aaggcgaggc aacgctgtac tggtttaaat ttaatccact atacaacgga atcgattgca
      301 aatctttccc actgttcttg aaagaatgcg aatttcgatt taacttcggc acaccgtctc
      361 aacagcttaa aatcctgcgg gattggtgtg ggatttaggg ctaatctagt acagcccctt
      421 ttaaaaaaca ttacaatccc gggccgccaa gcgaattggc aatgtccctg accgccgtta
      481 catacatccg gctgtggttg tactgccata ccgtataaaa attgttcaag cccaaataat
      541 attcaaacac gccgggtgcg gtttccagtt tgaacaaaac cgccttttca tcatcggcaa
      601 gctcttcgcc ggggatgatg ccgtacgcct tcaaatccgc caccgtccgc gtcagggcgg
      661 ttttttcgcc aatgattgcc tgaacatccg cacccggcgc caatgttgca gacaccagta
      721 ttttcccgcc cgtgcgccaa ccgtgctgct tcatataatt ggcaaccgat gccgcgacat
      781 cgccgatgtt gccccatatg tcccgatgtc cgtccccgtc ataatccacc gcccatttcc
      841 ggtagctcga aggcataaat tgcggcatcc ccattgcgcc cgcatagctg cctttaaagg
      901 cgaaaacatc gccgccttct tcttttgcca gctttaaaag ctcgaccaat tctttttgga
      961 aaaacccggc gcggcggggg taatcaaagc ctaaggtcgc caatgcgtcc gccacacgga
     1021 aactgcccgt atttttgccg taattcgttt caatcccgat aaccgccacg ataagttcgg
     1081 caggcacgcc gtatttttgc gccacatcat cgataagcgc gcggttttcc gcataaaacc
     1141 ggcgcgcgcc gcgaaatttc gccttgcccg aatttcccgt gcggaacaca taccacggac
     1201 gcgatgtgga ggggcggtgc ataatcttga cgatgtccgc cttgtaagcc gctttgtcaa
     1261 aaaaatcctg ccattccgcc cgggaaaaat cccctttccc gacttcatcg tccacaaaac
     1321 ggcggacatt tgcattggcg gcaaacccgc tgtcggatac cggtacggct gccgcgtcaa
     1381 atacggctgc cgcgtcaaac gcggggcggc tttctttttt catttcatcc gcgcgggggg
     1441 cttgggcttc atttgcccgg ggcgggcgtg cctccatcgc cgtacaggca gacaaagccg
     1501 ccaaacaaat tgccagcggc agtatttttc tctttttcat aaatatgttc cgaacaaata
     1561 gggtaagtgg gaaagcggcg caagggcgcg gcgcgaaatg cggtatccgc cgcaatcggc
     1621 ggctttgcag aatgccgcgc gttgcctttt gcgccgcccg aaatctgcct gccgccgccg
     1681 aaaatgccgt ctaaaggcac ttcccctttc agacggcatt gcctgcgccg tatttgcccg
     1741 ctatccgcaa tatcggcagt ccaatatgtc tttgcggata tcgttcagca ggcaggctgc
     1801 cgcgtcttcg tgttccgcct gtacgaagac aaagagtcgg gcgatgattt tgccgataaa
     1861 gactttttgc agggcgcagg cgtcggtcag cgtgtgttcc tgcaggaagc tgcggatgtc
     1921 ttcgggtaag gtgtaatcac gggcaacggc ggcaaagcgg gcatcggttt gcaggcggtc
     1981 gagcagggtt tggtttttgt ccaacttcat gcctgcttct ttttcttcgg cggtggcgcg
     2041 gacaaactgg tcgtaaattt cggcataaag catatcgttc gggccttcaa aaatcgtgaa
     2101 ggggcggatg tcgatagcga tattgccggc ggtgtgtccg cgttcaaaac ccttcgcgcc
     2161 caagagtttt tgcaacattt gcgcggcggc gtaagtgtat tccgtggcga gggttttgac
     2221 gatgttcgcc tccatcagct gatgggcgac gggggcaaca ggcgaaacgg aatggcagac
     2281 gtagcggtaa agaatctcgg aaacctgatg gcggcgccgg atttcgcggc gttcgtaatc
     2341 gacgaatctg atgtcgttgc ggacgtatcg ttccaggttt tcaaggatgt attccataat
     2401 gccgtgcgtc atgccgatca gttgcaggcg gctgcggata aagatgtttt ggaacgcgcg
     2461 caaaccggca gcgtcgctct gggagagttt catcacggcg gttgcaggca tttcggcatc
     2521 gatgcggttg acggcgtaac ggacggcgcg caagccttcg gatgcgaggg tttcgcagcg
     2581 gatgtatgtt ttggggacga gcagcaggtc gatgactttg gcgagtttgc cgtttttgcg
     2641 ttctttggcg gcaacgagga ggaagtcgct ttgcgagttg ccctgccagt attttgcggc
     2701 gttgacgtaa atggtttgtc cgtcggtata ttcgtagtaa gactgcattt ctcgggcaat
     2761 cgccgcgccg gaggtttcgg gttcggtaac gcccaaacca ccgccctcgc ctttgaaaac
     2821 catgtccaaa ccctgtgcga tttgcgcttc atcgccgaac tcttgcagtg gctgcaacac
     2881 cagcgcgcct tcgatgccgg tacgcagcgt aacgggcacg ccgtaatgcc ccgcaatccg
     2941 caagacttcc tgaatttcaa actggctgcc cttgcgcccg ccgtgttttt tgtcgaggaa
     3001 gggcaacagc aaacccgcct gcttcaaggc aagccatttg tcttcgggca ggtatcgcat
     3061 caggtcgata ccgtctgaaa aaatgcggcg gaatgcggat tcgatgtgct ttaaaaaagc
     3121 agccgtgtcc atagttgacg gctgcgcgct cggttcggtg tgtatcatcg gcttcctctg
     3181 tcggttccca ttaatcggcg gccggtcaaa ccgcctgcca cagtttagag ttgattttct
     3241 aaactttacc acaaagtgcg ccgggcaaca atccgccgac ctttcagacg gcatcgcgcc
     3301 ccctcccgtg ctaaaatgac cgtttgcatc actgtccgcc gattgccgca ctatgaccta
     3361 ccccatcccc aaaccccgtg aaaaatcccg ttggtccaat ctttcgcaag gctcgctgcc
     3421 cttggctttg gcgcgttatc tgccgcataa gcggctcaag gtcgtgctga cccaagatgc
     3481 ggaacaggcg ctacgccttc agacggcatg gcggtttttc cgtccgcacg acacggcggt
     3541 gttcctgccg gactgggaaa cgctgcctta cgagcgtttt tcgccgcatc aggatttggt
     3601 gtcggaacgg ctgtcggcgt tgtggcagat taagagcggc gcggcggacg tgttgttcgt
     3661 gccggttgcc acggcgatgc agaagctgcc gcccgtgccg tttctggcag ggcgcacgtt
     3721 ttggctgaaa acagggcaga ctttggatat aggccgtctg aaaaccgatt tggtagatgc
     3781 gggctacaac catgtttccc atgttgttgc ggcgggcgag tttgccgtgc gcggcggcat
     3841 tgtcgatttg ttccccatgg gcagcgaaat gccctaccgc atcgatttgt ttgacgatga
     3901 aatcgacagc atcaaaacct ttgatacgga cacgcagcgc accatctccc ccgtttccga
     3961 aatccgcctg ctgccggcgc acgaattccc caccgacagc gaggcgcaaa aaatcttccg
     4021 cagccgcttc cgcgaggaag tcgacggtaa tccgaacgat gccgccgtgt acaaagccgt
     4081 cagcaacggc cacttcggtg cgggcgtgga atattacctg ccgctgtttt ttgaaaacga
     4141 gctggaaacg ctgtttgact atatcggcga ggatgcgctg tttgtctctt tgggcgatgt
     4201 tcacgccgag gcaaaccgct tttggagcga cgtcaaatcg cgttacgcca tggcgcaggg
     4261 cgatgaaact tatccgcctt tgcttccaca gcatttgtat ctctctgccg atgtattcgc
     4321 aggccgtctg aaaaactacg gacaagtgct gcccgatgtt tccggcaagg aacacatcct
     4381 gcccgacctt gccgtcaacc gccaatccga cgagccgttg caggcattga aggattttca
     4441 gacgaccttt gacgggcgga ttttgctgtg cgccgaaagc ttgggacggc gcgaaaccat
     4501 gctcggtttc ttgcagcaaa acggtttgaa agccaaacct gtgtccgact ggcagggctt
     4561 cttgtcggcg catgagccgc tgatgattac agtggcgccg ttggcttacg ggtttaaact
     4621 gggcggactg caatcgccga accaacagca aactacttcc gcctccgaag gagaaggcga
     4681 tgcggttacc gaccaaaccg aattttccgc atccgcaaca aaccctctcc ctagccctct
     4741 cccacaggag agggaacaaa gtgcagccg
//
LOCUS       DAWFLT010000117         4651 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWFLT010000117 DAWFLT010000000
VERSION     DAWFLT010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.117"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(713..1138)
                     /locus_tag="WGR74_002011"
                     /pseudo
     CDS             complement(713..1138)
                     /locus_tag="WGR74_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            1286..1738
                     /gene="dut"
                     /locus_tag="WGR74_002012"
     CDS             1286..1738
                     /gene="dut"
                     /locus_tag="WGR74_002012"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ0906350.1"
                     /translation="MNIEVEMKVLDERMADFIPTYATAGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPSYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVIVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            1814..3001
                     /gene="dapC"
                     /locus_tag="WGR74_002013"
     CDS             1814..3001
                     /gene="dapC"
                     /locus_tag="WGR74_002013"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ0906351.1"
                     /translation="MNTLLKQLKPYPFARLHEAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTVDADNEILPVLGSREA
                     LFSFVQTVLNPVSDGIKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAEDIVS
                     LYR"
     gene            3159..3938
                     /gene="yaaA"
                     /locus_tag="WGR74_002014"
     CDS             3159..3938
                     /gene="yaaA"
                     /locus_tag="WGR74_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ0906352.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESEILMQQLRKLAPQ
                     QIAELMHVSDKIALLNAERNAEWHTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLMQPYRLEMGTPFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKTLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            4021..4111
                     /locus_tag="WGR74_002015"
     tRNA            4021..4111
                     /locus_tag="WGR74_002015"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4055..4057,aa:Ser,seq:tga)
     gene            4189..4278
                     /locus_tag="WGR74_002016"
     tRNA            4189..4278
                     /locus_tag="WGR74_002016"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4223..4225,aa:Ser,seq:cga)
     gene            complement(4474..>4651)
                     /locus_tag="WGR74_002017"
     CDS             complement(4474..>4651)
                     /locus_tag="WGR74_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0906353.1"
                     /translation="KFNPLYVVEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLN
                     LLKAANRLSAPAAA"
BASE COUNT         1195 a         1123 c         1131 g         1202 t
ORIGIN      
        1 gcgggaatga cgaatccatt cgtacggaaa cctgcaccac gtcattccca cgacagtggg
       61 aatccagttt tttgagtttc agtcattccc gataaagtgc cttagcattg aatgtctaga
      121 ttcccgcctg cgcgggaatg acggatttta gtttgggggg catttattgg aaaaagcaga
      181 aaccgctccg ccgtcattcc cacgaaagtg ggaatccagg atgcagggtt ggggaaaccg
      241 tgttatccga taagtttccg caccgacagg tctagattcc cgcctgcgcg ggaatgacga
      301 acctatccgt acggaaacct gcaccgcgtc attcccacga aagtgggaat ctagtttttt
      361 gagtttcagt cattcccgat aaattgcctt agcattgaat gtctagattc ccgcctgtgc
      421 gggaatgacg aatccattcg tacggaaacc tgcaccacgt cattcccacg aaagtgggaa
      481 tctagttttt tgagtttcag tcattttcaa taaattgcct tagcattgaa tgtctagatt
      541 cccgcctgcg cgggaatgac gattcatata gtggattaac aaaaatcagg acaaggcggc
      601 gaagctgcag acagtacaga tagtacggaa ccgattcact tggtgcttca gcaccttaga
      661 gaatcgttct ctttgagcta aggcgaggca acgctgtact ggtttttgtt aatccactat
      721 aaaaaggcat attgaatgcg ggcaaaccgg ctgctttccg tttttggatt tcggagaatg
      781 ccatcgccca gctttcatca cacataaaaa acagtgcggg cacggctttt ttcagcggta
      841 tttctttcag gtgcagggca agtgccgccc ccatcaggat atgccgcgaa ttaatcataa
      901 aggttacggt ggcgataagc agtatcggca gagattccgc ccacaggttg accgtggcaa
      961 actcggagcc gccggcgaag ttcatactgg tcatcaacaa catttccagc cagctcatgc
     1021 ctttttgtcc gccctgcata ccgagtatta atgcccaagg cagaagtccg ataagtatgg
     1081 gcgaactttc tttgatgccg cgtataaatt cgttacgggg cgagcttatg tgtgtcatat
     1141 tataatattg atatgtaaga aggtcgggca ttatacacag acggagtatt tcaaaacaaa
     1201 acctatttgc tgtgtttcag tgtaaacacg gcttgtgtat aatccaccat ctttagacag
     1261 accgaccgta tgtgggagca aggcaatgaa tattgaagta gaaatgaaag tgttggacga
     1321 gaggatggcg gattttatcc ctacctatgc gacagcgggt tctgccggtt tggacttgcg
     1381 cgcctgtttg gatgaggaag tcgtcttgca gccgggggaa acgtttcttg tgccgacagg
     1441 tttggcaatt tatttggcca atccttcata tgccgccgtt ttgctgcccc gttccggctt
     1501 ggggcataaa cacggcattg ttttgggcaa tttggtcggc ttgattgact ccgattatca
     1561 aggggaattg aaggtttcgt tatggaacag aagcagcgag ccgtttactg tcaaaccgtt
     1621 tgagcgtatc gcgcagatgg ttatcgtgcc aatcgtgcag gcgcgtttca aacgtgtcga
     1681 ggaatttgtc ggaagcagcc gtggagaagg cggtttcggc agtacgggtt tgcattgaat
     1741 ataaaatgcc gtctgaaggg cacgtcaggt tcagacggca tatcttccga tataccgata
     1801 accggagaaa atcatgaata ccttactcaa gcagctcaaa ccctatccct ttgcccgact
     1861 gcacgaggcg atgcagggca tttccgcgcc tgaaggcatg gaagccgtcc ccctgcacat
     1921 cggcgaaccg aaacatccga caccgaaagt cattacggat gcgctgaccg cctcattgca
     1981 cgagttggaa aaatatccgc tgactgcggg tctgcctgaa ctgcgtcagg cgtgtgcaaa
     2041 ctggttaaaa cgccgttacg atggcttgac agtggatgcg gataatgaaa ttctgccggt
     2101 tttaggcagt agggaggcgt tgttttcttt tgttcaaacc gtgttgaacc ctgtttcaga
     2161 cggcatcaaa cccgcaattg tcagcccgaa tcccttttat cagatttacg aaggtgcgac
     2221 acttttgggc ggcggtgaaa tccattttgc caattgcccc gcgccgtctt tcaaccccga
     2281 ttggcgtagt atttccgaag aggtttggaa acgcaccaaa ctggtgttcg tctgctcgcc
     2341 caacaacccc agcggcagcg tgctggattt ggacggatgg aaagaagttt ttgatttgca
     2401 ggacaaatat ggtttcatta ttgcctcgga cgaatgctat tccgaaatct atttcgacgg
     2461 caacaaacct ttaggctgcc tgcaggccgc tgcacagttg gggcgaagca ggcaaaaact
     2521 gcttatgttc accagtttgt ccaagcgttc caacgttccg ggcctgcgtt ccggttttgt
     2581 cgccggcgat gccgaactgc ttaaaaactt tctgctttac agaacctatc acggcagtgc
     2641 aatgagcatt cccgtgcagc gcgcaagcat tgccgcttgg gatgacgaac agcacgttat
     2701 cgacaaccgc cgcctgtatc aggaaaaatt tgagcgcgtt attcccattt tgcaacaggt
     2761 atttgacgtt aaattaccgg atgcctcgtt ttacatctgg ttgaaagtcc ccgatggcga
     2821 cgatttggca tttgcacgca atttatggca aaaagccgct atccaagtat tgcccggacg
     2881 ttttttggcg cgggataccg aacagggcaa tcccggggaa ggttatgtcc gtatcgcttt
     2941 ggttgccgat gtcgcaactt gtgtcaaagc tgcggaagac atagtttcac tgtatcggta
     3001 aggaatcggc aatgcagtct gaacttttgt tcagacggca tttttcaata ttttacggtt
     3061 gaatttgctg taacggtatt tacttcactt ttaatcggct tgcccgcaaa cacgtttaaa
     3121 cttaaaatcc ccatgtttga cacaataccg agcggattat gttttttgtc ctttcccctg
     3181 cgaaaaacct taacgaaaaa gaccctgccc ctgtcagtga gtttacccaa cccgacctgc
     3241 tggcagagtc cgaaattcta atgcagcagt tgcgcaagct tgcgccgcaa cagatcgccg
     3301 aactgatgca cgtttccgac aaaatcgcac tcttaaacgc cgaacgaaat gcagaatggc
     3361 acacgccgtt tacgccggaa aacgccaaac aggcggtctt tatgttcaac ggcgatgttt
     3421 acgaaggtat ggatgcaaac acattggata ttggacagat acgctatctg caaaaccatg
     3481 tccgcctgct gtccggtctg tacggtcttc tgcgtccact ggatctgatg cagccctacc
     3541 gcctggaaat gggaacgccc ttcgccaatt tgcgcggcaa gaatttgtat gagttttggg
     3601 gcgacatcat taccaacctt ttaaatgaca cgcttgccca agcaggcagc aatacgcttg
     3661 tcaatcttgc ctcacaggaa tattttaagt ccgtcaacac gaaaacactt cgggcgcggc
     3721 tgattacccc gatatttaaa gacgaaaaaa acggcaaata taaaattatc agtttctatg
     3781 ccaagcgtgc acgcggatta atggtgcgct atgcggcaga acatcatatt accgaccctg
     3841 aaatgctgaa aaattttaat tacgaaggct acgcattcaa tgacgcggct tcaaatgaaa
     3901 gcgaatgggt ttttatgcgt tcggaacaaa taaagtgaaa acaataaatt aagtattttc
     3961 caaaaaaagt gcttggcaaa atgtataaac ttcattatta ttcctaatct tcaagaagac
     4021 ggaagcgtgg cagagcggtt taatgcaacg gtcttgaaaa ccgtcgaggg ttgatagccc
     4081 tccgtgagtt cgaatctcac cgcttccgcc aatccttgag gtcaaaaacc aaataaaaat
     4141 gaagtagcat aaagcatcgg catttttatt tggttttact tcattatcgg aaacgtggca
     4201 gagaggctga atgcagcgga ctcgaaatcc gctgagggtg caaatcctcc gtgggttcga
     4261 atcccaccgt ttccgccaca aaacaaaacc gccctgattc ggggcggttc ttttttgttc
     4321 aagttgagac ctttgcaaaa ttcccttccc tcccgacagc cgaaacccaa acacaggttt
     4381 tcggctgttt tcgccccaaa taccgcctaa ttttacccaa atatcccctt aatcctgccc
     4441 ggatacccga taatcaggca tccgggctgc cttttaggcg gcagcgggcg cacttaacct
     4501 gttggccgct ttcaacaggt tcaaacacat cgccttcagg tggctttgcg cactcacttt
     4561 aatcagtccg aaataggctg cccgagcgta gcggaattta cggtgcagcg taccgaagct
     4621 ctgttcgacc acatatagtg gattaaattt a
//
LOCUS       DAWFLT010000118         4563 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWFLT010000118 DAWFLT010000000
VERSION     DAWFLT010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.118"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            22..256
                     /locus_tag="WGR74_002018"
                     /pseudo
     CDS             22..256
                     /locus_tag="WGR74_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
     gene            424..2199
                     /gene="ggt"
                     /locus_tag="WGR74_002019"
     CDS             424..2199
                     /gene="ggt"
                     /locus_tag="WGR74_002019"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ0906354.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            2418..2613
                     /locus_tag="WGR74_002020"
                     /pseudo
     CDS             2418..2613
                     /locus_tag="WGR74_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2680..3930
                     /locus_tag="WGR74_002021"
     CDS             2680..3930
                     /locus_tag="WGR74_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ0906355.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVREQVTALCNKYPVYGA"
     gene            4058..4563
                     /locus_tag="WGR74_002022"
                     /pseudo
     CDS             4058..4563
                     /locus_tag="WGR74_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1256 a         1130 c         1058 g         1119 t
ORIGIN      
        1 aggcacgccc cagtttcccc aatgtggatt ttcagcgcgt gcagtagagg tgctgaccca
       61 attggcagac cctttgcttt tgttaatatc ctagaaaacc cagaaattcg cagtactcta
      121 ccactcatcg ccaatcggcc aacctttcca cagctttggg ttaatggtga gttaatcggt
      181 ggtagtgata ttatcacaaa tgtaccaatc aggtgagcta aaaccactca ttgaagagca
      241 tagcccaaag gtctgattac ttaatatctt aaaataaata tcaaaaaaga ctgctgatga
      301 cattagcggt ctttttttga tggtcaagat tgatagtttg gattgaatta tttagtcata
      361 ttaaatacaa taatcagcat gccttgtatg aatcatcaat caaactcagg cgaaggagtg
      421 cttgtggcta aaacatattt attgactgca ttgataatgt ctatgacaat ctctggatgt
      481 caagtcatcc atgccaatca aggtaaggtt aatactcatt ctgctgtcat cacaggtgca
      541 gacgctcaca cgcctgaaca tgcaacggga ctgaccgaac aaaagcaggt gattgcaagt
      601 gattttatgg tagcgtcagc caatccatta gcaacacaag ctggctatga tatcttaaag
      661 caaggcggta gcgctgcaga tgcgatggtg gcggtgcaga cgacactaag cttggtagag
      721 ccacagtcgt caggcttggg cggtggtgca tttgtgttgt attgggataa taccgccaaa
      781 acattgacca catttgatgg gcgtgagacg gcaccgatgc gtgcgacgcc ggaattattt
      841 ttggataaag atggtcaacc attgaaattt atggaagcgg tggtcggtgg tcgctcggtg
      901 ggtacgcctg ctatccctaa actgatggag acaatacatc agcgatacgg tgtattgcct
      961 tggggaaaat tatttgatac accgatccgc ttggcaaaac aaggctttga ggtgtcgcca
     1021 aggcttgcca tctcggttga gcaaaatcag cagcatttgg cacgctatcc aaaaacagcc
     1081 gcttattttt tgccgaatgg tgtgccgcta caagcaggca gcttgctgaa aaatttagaa
     1141 tttgctgaca gtgttcaggc gttagcagct caaggtgcaa aagctctgca tactggtaaa
     1201 tatgcccaaa atatcgtttc agttgtccaa aatgctaagg ataaccccgg tcaattatcc
     1261 ttacaagatt tatctgatta tcaagtggtg gaacgcccgc ctgtttgtgt gacttatcgt
     1321 atttatgaag tatgcggtat gggtgcacca agctcaggag ggattgctgt gggtcagatt
     1381 ttggggattt taaatgaatt ttcacccaat caggtggggt atgatgctga aggtttacgt
     1441 ctcttgggcg acgcttctag gcttgcgttt gcggatcgtg atgtatattt gggcgaccct
     1501 gattttgtac cagtacccat tcgccagttg atttctaaag actacctaaa acatcgcagc
     1561 cagctgcttg agcagtcgga taaggcattg cctagcgtat ctgcaggcga ttttattcat
     1621 gagtgggtgt cctcacaagc gattgagtta ccttctacca gtcatatatc aattgtggat
     1681 aaggcgggca atgtgctatc aatgacgact tccattgaaa atgcgtttgg ctcaactttg
     1741 atggcaaatg gctacctgct gaataatgag ctgacagact ttagttttga gccaataaag
     1801 cagggcaaac aggtcgccaa tcgtgtcgag cccggcaaga gaccacgctc ctcaatggca
     1861 ccaaccatcg tatttaaagc aggcaagcct tatatggcaa tcggctcccc aggtggtagc
     1921 cgtatcattg gctatgtcgc taagacgatt gtggcacata gtgactggaa tatggatatc
     1981 caagatgcca tcagtgcacc caatttatta aatcgctttg gtagctatga actagaaacg
     2041 ggtacaaccg ccctccagtg gcaacaagca ttaaatgatt tgggctataa gactgacgtg
     2101 cgtgagctaa attcaggtgt tcaagccatt attatcgagc caagcaggct ggttggtggt
     2161 gccgatccac gccgagaagg tcgagtgatg ggtgattaga ggtgaagctt gtattttgtg
     2221 aatttgaccg cattaagtta atattaaaat agggggaaat tgtgagtaat caaacaaaaa
     2281 tacaagccat caagcaagct tctgagcaga tcttcgcaat ttgtgagacg ccaaacacag
     2341 cattacaagc cattcatctg attttaagac atggtggggc aggagagttg tcttggcaag
     2401 tggtgtatca gcgggttatg gctgataaag atgtggttgg tgctggttat ttgattgatt
     2461 ttgcacaaac tgccgaaaat ttaccatttg atgcattgcc tttgggtagc cttgtactaa
     2521 cataaaggta atgagacgct aaaaaccagc atgttaaata aattacctga taatgccaaa
     2581 gaaaatttac gcattatgga ctatctgcct taggttttga agtatttttt tatacaaaaa
     2641 acactcatga taccctgatt acacaaagga gccgagaaca tgttttcaaa aagcgttacc
     2701 ctcgcacaat atgaccccga tttggcagca gccattgccc aagaagacca acgccagcaa
     2761 gaccacgtcg agctgattgc ctctgaaaac tacgtcagct gcgccgtaat ggaagcgcaa
     2821 ggttcccagc tgaccaacaa atacgccgaa ggctaccccg gcaaacgcta ctacggcggc
     2881 tgcgaatacg tcgatattgt cgaacaattg gcgattgacc gcgtcaaaaa actgttcggc
     2941 gcgcaatacg ccaacgtcca accgcactcc ggctcgcaag ccaaccaagc cgtgtacgct
     3001 tccgttttaa aaccaggcga caccattttg ggtatgtctc tggcgcacgg cggccacctg
     3061 acccacggcg cgagcgttaa tatctcaggc aaactctaca acgctgttac ttatggtttg
     3121 gatgaaaacg aagtgctcga ttatgccgaa gtcgaacgcc tcgcgctcga acacaaaccc
     3181 aaaatgattg tggcgggtgc gtctgcctac gcgttgcaaa tcgactgggc aaaattccgc
     3241 gaaatcgccg ataaagtcgg cgcatacctc tttgtcgata tggcgcacta tgccggtctg
     3301 gttgccggcg gcgaatatcc caatcccgtg ccgttctgcg acttcgttac caccaccacc
     3361 cacaaaaccc tgcgcggtcc tcgcggcggc gtaattttgt gccgcgacaa tacccacgaa
     3421 aaagcgttga actcttccat cttcccaagc ctgcaaggtg gcccgctgat gcacgttatc
     3481 gccgccaaag ccgtggcgtt taaagaagca ttgcaacccg agttcaaaca atacgcaaaa
     3541 caagtgaaaa tcaatgcagc cgccatggcg gaagagttgg ttaaacgcgg tttgcgcatc
     3601 gtttccggcc gcaccgaaag ccacgttttc ctcgttgacc tgcaaccgat gaaaattacc
     3661 ggcaaagccg ccgaagctgc tttgggcaaa gcgcacatca ctgtcaacaa aaacgccatc
     3721 ccgaatgatc cggaaaaacc cttcgttacc tccggcatcc gcatcggttc cgccgccatg
     3781 accacacgcg gttttaacga agccgacgcg cgcgtattgg cgaacttggt tgccgacgta
     3841 ttgtctaacc ccgaagacga agccaacctc gccaaagtcc gcgaacaagt aactgctttg
     3901 tgcaataaat accccgttta cggcgcgtaa gcctttttaa aaatattccg ctaagcaatc
     3961 caatgccgcc tgaaatctca taatgtttca ggcggaaacc tttgcaaaat tccccaaaat
     4021 cccctaaatt cccaccaaga catttagggg attttccatg agcaccttct tccggcaaac
     4081 cgcgcaagcc atgattgcca aacatatcaa ccgtttcccg ctattgaagt tggaccaagt
     4141 gattgattgg cagccgatcg aaccatacct gaaccgtcaa agaacccgtt acctccgaga
     4201 ccaccgcggt cgtcccgcct atcccctgct gtccatgttc aaagccgttc tgctcggaca
     4261 atggcacagc ctctccgatc ccgaactcga acacagcctc atcacccgca tcgacttcaa
     4321 cctgttttga cgttttgacg aactgagcat ccccgattac agcaccttat gccgctaccg
     4381 caaccggcga cgcaagacga caccctgtcc gaattgccgg aactgatcaa ccgccaactg
     4441 accgaaaaaa acctaaaaat agagaaagca tccgccgccg tcgttgacgc caccattatt
     4501 cagaccgaga cctttgcaaa attcctttcc ctcccgacag ccgaaaccca aacacaggtt
     4561 ttc
//
LOCUS       DAWFLT010000119         4214 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWFLT010000119 DAWFLT010000000
VERSION     DAWFLT010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.119"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(84..1130)
                     /gene="recA"
                     /locus_tag="WGR74_002023"
     CDS             complement(84..1130)
                     /gene="recA"
                     /locus_tag="WGR74_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ0906356.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEVANEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            1384..2148
                     /locus_tag="WGR74_002024"
     CDS             1384..2148
                     /locus_tag="WGR74_002024"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ0906357.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEILHHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRCAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLKAKELAAKPIVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRATLDCLENGAD"
     gene            2168..4183
                     /gene="rep"
                     /locus_tag="WGR74_002025"
     CDS             2168..4183
                     /gene="rep"
                     /locus_tag="WGR74_002025"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ0906358.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSKLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLLASEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDSMKKGGPED"
BASE COUNT         1002 a         1154 c         1156 g          902 t
ORIGIN      
        1 caaaactcaa atcaagccgt tcggaggcgg ctcaaaaaaa cggtacttcg cagcagaagt
       61 accgtttatc gggatttcag gttttattct tcggggcgtt cgccgtcggt ttcgtcctgc
      121 gtcccttcgg tgatgtgcat ttctacgccg ttgagggcgc ggatttttgc gtcgatttca
      181 ttggcgactt cgggattttc cttcagccag acgcggacgt tgtctttgcc ctgaccgatt
      241 ttcgcgccgt tgtagctgta ccacgcgccg gatttgttga tgatgtcgtt tttcacgccg
      301 atgtcgatca attcgccttc ccaactgatg ccttctccgt agaggatgtc aaactctgcc
      361 tgacggaacg ggggggcgac tttgtttttg atgactttga cgcgggtttc gttgcccaat
      421 acctcttcgc cttttttgat ggatccggtg cggcggatgt cgaggcggac ggaagaatag
      481 aatttcagcg cgttgccgcc ggtggtggtt tcggggctgc cgaacattac gccgatcttc
      541 atccggattt ggttgatgaa cacaaccagc gtgttggttt ttttgatgtg tccggtcagt
      601 ttgcgcaaag cctggctcat caggcgcgcc tgcagtccga catggctgtc ccccatatcg
      661 ccttcgattt cggctttggg gacgagtgcg gctacggaat cgacgactac catatctatg
      721 ccgcccgaac ggacgagtgt gtcgcagatt tccaaagcct gttcgccggt atcgggctgg
      781 gacaggtaaa gctcttcgac ttttacgccg agtttgcggg cgtaaacggg atcgaaggcg
      841 tgttcggcat cgacaaaggc gcacacgccg ccgtttttct ggcattgggc gacggcttcg
      901 aggcagaggg tggttttgcc ggaggattct gggccgaaga tttcgacgat gcgcccgcgc
      961 ggcagaccgc cgactccgag ggcgaggtct aatccgagcg atccggtgga aatgacttcg
     1021 aggttttctt cctgctggct gccgtccatt ttcatgatgg cgcctttgcc gaaacttttt
     1081 tcgatttgcg ccagtgcggc ggcaagtgct ttgcttttgt cgtctgacat tggggttact
     1141 ccggaacaaa tgcggtatgt gggatgcggc gcaacacggg ctgcggcgcg ggatgtgtat
     1201 cgttttcccg atgtgcgggc tatcggtaat gctgcttcac gaggttgcca ttatcgcata
     1261 tttccttgct tgccgatatg cggcaggacg cggcggcttg tgccggaatg gaatctggat
     1321 gccgtctaaa aggcggccgg ctttgttata atggcggctg ttttttctgt gtgtgcctgt
     1381 tttatgtgtt cctgccttgt tgtcaaaaat accgttatcg gaagcggacg caccaaaatc
     1441 gccgtgccgc ttgtcgcccg cgatgccgcc gaactttccg ccgtacttga gcaaatcaaa
     1501 aatatgccct tcgatattgc ggagttccgc gccgactttt tggaatgcgc gggcagtatc
     1561 ggcgaaatat tgcaccacac gcagaccgtc cgcgacgcgc tgcccgacaa gccgctgctg
     1621 tttacgttca gacggcatgg cgaaggcggc tcgttcccgt gttcggacga ttattatttt
     1681 gaactgctcg acgcgctgat cgaaagccgc ctgcccgaca tcatcgacat cgagctgttt
     1741 tccggcgaaa ccgccgtccg gtgcgccgtg gcaaatgctc aaaaaaacgg catcgccgcc
     1801 ctgctctgca atcatgagtt tcaccgcacg ccgccgcaag aagaaatcgt atgccgtctg
     1861 aaacagatgg aggactgcgg cgcggacatc tgcaaaattg cggtgatgcc gcaaagcgcg
     1921 gaagatgtgc tgactttgct ttccgccacg ctcaaagcga aagagcttgc cgccaaaccg
     1981 attgttacga tgtcgatggg gcagacgggg gcggtcagcc ggcttgccgg acaggtgttc
     2041 ggctcaagca tcacgttcgg ttcgggaacg caaaactccg cgccggggca aatcggcgta
     2101 tccgccctcc gtgcgacact cgactgcctc gaaaacggag cagactgatt tcagacagca
     2161 tcaaaacatg atgaaactca atccccaaca gctcgaagcc gtccgctacc tcggcggccc
     2221 actgctcgtc cttgccggtg caggcagcgg caaaaccggc gtgattactc aaaaaattaa
     2281 gcatttgatt gtcaatgtcg gctacctgcc gcataccgtt gccgcaatta cctttaccaa
     2341 caaagccgct gcggaaatgc aggagcgcgt tgccaaaatg ctgcccaaac cgcaaacgcg
     2401 cgggctgacg atttgcacgt tccactcttt gggcatgaag attctgcgcg aagaggcgaa
     2461 ccatatcggt tacaaaaaaa acttctccat tctcgattct accgacagcg cgaaaatcat
     2521 cggcgaactc ttaggcggca cgggcaaaga agccgtattc aaggcgcagc accagatttc
     2581 cttgtggaaa aacgatttaa aaacgcctga agatgtcgtt cagacggcat cgaacatttg
     2641 ggaacaacaa accgcacgcg tgtatgcgag ctatcaggaa accttacaaa gctatcaggc
     2701 agtggacttc gacgacttga tccgcctgcc tgccgtgctg ttgcagcaaa acagcgaagt
     2761 gcgcaacaaa tggcagcggc ggctgcgtta tctgctggtt gacgaatgcc aagatacgaa
     2821 tacctgccaa tttacgttga tgaaacttct gaccggcgcg gaaggtatgt ttaccgccgt
     2881 cggcgacgac gaccagtcca tctacgcatg gcgcggcgcg aacatggaaa acctgcgcaa
     2941 aatgcaggaa aactatccgc agatgaaggt catcaaactg gagcaaaact accgctccac
     3001 cgcgcggatt ctcaaaatcg ccaacaaagt catcgagaac aaccccaagc tgttcaccaa
     3061 aaaactttgg tcgaaattgg gcgaaggcga gcccgtcaaa gtcgttgcct gccaaaacga
     3121 gcagcacgaa gccgactggg tcgtcagcca aatcgtcaaa caaaaactca tcggcggcga
     3181 caaaacccaa tatgccgatt tcgccgtgtt ataccgcgga aagcatcagg cgaggatttt
     3241 cgaggaagca ttgcgcggcg cgcgcatccc ctaccgtctc tccggcggac aaagcttttt
     3301 cgacaaagcc gaaatcaaag acgtgttgtc ttatgtgcgg ctgcttgcca accccaacga
     3361 cgatcccgcc tttctgcgtg ccgttaccac gcccaaacgc ggcatcggcg atgtaacgct
     3421 gggcaagctc aacacttacg cgcacgaaca cgaatgcagc ctgtatgaag ccgcgcaaaa
     3481 cgaagaagcc ctcgccacgc tgaacaatac caaccgccaa cacctgcaag cctttatgga
     3541 tatgttcggc agttaccgtg ccaaagccga aaccggcgaa gcgggcgagt tcatcaacag
     3601 cctgctcgaa gaaatcgact atgaaaacca cctgcttgcc agtgaagaag gcaaagctgg
     3661 cgaaatcaaa tggcgcaacg tcggcgattt ggtatcatgg tttgcgcgaa aaggcgggga
     3721 agacggcaaa aacatcatcg aactcgccca aaccgtcgcc ttgatgacgc ttttggaagg
     3781 aaaagacgaa gaagaaaccg atgccgtctc gctatccacg ctacacgccg ccaaaggttt
     3841 ggagtatccg tatgttttcc ttgtcggttg cgaagaaggc gttttgccgc acaacgacag
     3901 catcgaagaa ggcaacgtcg aagaagaacg ccgcctgatg tacgtcggca tcacccgcgc
     3961 caaacgccaa ctcacactga cccactgcgt caaacgcaaa aaacaaggca catggcagtt
     4021 ccccgaaccc agccgattca tagacgaaat gccgcaggaa gatttgaaaa tcctgggtcg
     4081 caaaggcggc gaaccgattg tcagcaaaga agaaggcaga cgcaaccttg ccgatataat
     4141 cggaaggctc gacagcatga aaaaaggcgg tccggaggat taaaccaaga tagggaaacg
     4201 aagccggaga caat
//
LOCUS       DAWFLT010000120         4111 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWFLT010000120 DAWFLT010000000
VERSION     DAWFLT010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.120"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..243
                     /locus_tag="WGR74_002026"
     CDS             <1..243
                     /locus_tag="WGR74_002026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906359.1"
                     /translation="RLKTEGGSLSSLPSDGISNAAFAPYAGGSPPSFQCLRRMPVASG
                     KPCAGWLCRLKQYEGMRFRRHPPVAEIPSASCPIIA"
     gene            complement(233..1426)
                     /locus_tag="WGR74_002027"
     CDS             complement(233..1426)
                     /locus_tag="WGR74_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ0906360.1"
                     /translation="MNPKHLIAFSALFAATQANALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLQSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNSGDFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTRAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTHWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIHDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQHMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(1423..2391)
                     /locus_tag="WGR74_002028"
     CDS             complement(1423..2391)
                     /locus_tag="WGR74_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ0906361.1"
                     /translation="MKHFLSLFTSILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYAFSDGIKTFLSGETPPTAQDGGSADMPSEAVPQTGETEWKQD
                     TEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPSFFDWP
                     KTIEETLQKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVERILEAAH
                     THRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLKGKIILIPTAHTLSGGEDRYTDSV
                     NVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            complement(2402..3838)
                     /locus_tag="WGR74_002029"
     CDS             complement(2402..3838)
                     /locus_tag="WGR74_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ0906362.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGQGGAIDILMPLGLSYYTFQSIAYLVYCFRAPHAARFEWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLGGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWFVTFHFVCLTFVVFNTANPD
                     DAGAVFNALFANANGWNAPQRADMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVFAPSGIPGFIYANF"
     gene            complement(3968..>4111)
                     /locus_tag="WGR74_002030"
     CDS             complement(3968..>4111)
                     /locus_tag="WGR74_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEZ0906363.1"
                     /translation="NVGIGSGALLGHWVTQYVGISRIGIAGMLAAAAGLWLCLNLNRH
                     IQT"
BASE COUNT          850 a         1042 c         1332 g          887 t
ORIGIN      
        1 cgtctgaaaa cggaaggcgg cagtttatca tctttgcctt cagacggcat atcaaacgcc
       61 gcctttgcgc cgtatgccgg aggttcgccc ccgtccttcc aatgcttgag gcgaatgccg
      121 gttgcgtccg gcaaaccgtg cgcggggtgg ctatgccgtc tgaagcaata tgaaggtatg
      181 cgtttcagac ggcatccccc tgttgcggaa ataccgtccg cctcctgtcc gattattgcc
      241 tgattgcagc ggcgcggacg agttcttcga ggctgtcggc gagcatttcc gccgcgcgcc
      301 ggtagccttt ggcggagaag tgtacgccgt ctttggcggc ccatccttgg ttgagccagt
      361 ttttcatgct gcatatgccg cccatggcgt tttgccaaga ccagaacatg gtttgtccct
      421 gccgggcgac gcgccgctgc atatgttgga cttcggtcag gcggacgggg cgtgtgccgc
      481 atacgccgag cgtgtttttc agggattcgg gtgcgccgat gatgaggatg ccggcggcag
      541 gcaggctgtc gtggatttgg cggacggtat ccagccattt ttgttcagtg tcggcaatgt
      601 cgatgttgtc gccgaaggct tcgttggtgc cgtaggaaag gataaccaaa tcggcgccgg
      661 tttgggcaag gtcgttcata cggtcggcac gccatttcga ccaatgggtc aattgtgcgc
      721 cgttgatgcc catggcggaa acggtaatgc cgccggcggg attttcgatg ttgatgaagc
      781 cgatgtccca cggcatttcg gtctgtatgg tcaagggcag tgccgcgcgg gtatccagta
      841 cctgccagcc gccgccgttg gcggagacgg tgttgccgtt gacggtcagg gtttgttcgg
      901 cgagcagggg tttggcaaac agggaaatgc gctgtttgcc ggtcttgccg tccgatgcgg
      961 tcagggtcat gctgccgccg ctgccggtgt gggcgaggat gccgccgagc gggaagtctc
     1021 cgctattgtt cctgctggtg aggctttgcc agttaccgtt gtgccggacg gccgccatgc
     1081 gctgcccttt gacgttggcg gggtaaaccc agcctatgcc gccgtcgccc caagtttttt
     1141 gcaggcgttt gcgcaggctg tcggtaaaga agtcgccggc ggtatgcgag tcgccgattt
     1201 gcaggatgcg gaaggtctcg ccgctgcctt gtgcgacgga ttggagtttt ttcatccaag
     1261 gcgaggcggc ggcgttgccg tagtcggtca gcagcccgtt tgtatcggtg tagggggcgg
     1321 acggggaaac ggtaacggtg tcgagactga cggaggcaac aggtagggcg tttgcctggg
     1381 ttgcggcgaa cagggcggaa aatgcgatga ggtgtttggg gttcatggct gtgtacttga
     1441 tggttgcgta cttggttcaa aaacgatttt ttccattatt tttgccgcca gcagtttttg
     1501 tccttcggcg gtaaagtgta tgccgtcctt gctgcggtag cggacgggtt tgccgttgac
     1561 gttgacggag tccgtgtagc ggtcttcccc gccgctcagg gtgtgcgcgg tgggaatcag
     1621 gatgattttg cctttcaaat gttccgaaag cagtttgtcg aggtagcgca tctgcccgtc
     1681 aagcttggct tttttcatgt aggggatgcc gagccagacg acttggacgc ggtgcgtgtg
     1741 tgcggcttca aggatgcgct cgacgcgttt caggtattct tgcgcccatt cgtcggaagc
     1801 aaatttgagg tagcgtttgc cgacgggaaa atcccacggg tcgttcgggc cgaggaagac
     1861 ggcgagtacg ctgatttcgg gatgtttttg cagggtttct tcaatcgttt tcggccagtc
     1921 gaagaatgag gggtaggaca gccccgtgct ttgtttgctg aggttgacgg attcgatgcc
     1981 gtattgctgt ttcaggcttt tttgcacgaa gggtgcaacg ccctgcatca gcgagtcgcc
     2041 ggcgaaaaag actttgtcgc cgctgcggac ggcggcggct tcggtgtctt gtttccattc
     2101 tgtttcaccg gtttgaggga cggcttcaga cggcatatct gccgagccgc cgtcttgagc
     2161 cgtcggcggc gtttcgccgg acaggaatgt tttgatgccg tctgaaaagg cgtaggcgtt
     2221 ttcttgcaac gccgcaccgc tccgccacca tccgtaggcg gcaagcggtt cgagcggact
     2281 gttgcggtgg taggtctgct gccagtaggc gttgatgggg ttttggctga accacacggc
     2341 aatcagggca gacatcaata tggaagtgaa aagggaaaga aagtgtttca tgtccaaacc
     2401 cttaaaaatt ggcataaata aagccgggaa tccccgaggg ggcgaatacg ataatcagca
     2461 gcaggacggc ggaaacgggg ataaaccaca gccacatcgg gattttttcc aaacctttga
     2521 ccgcgccgtc gaaagcgcgt tgcaggtaag ggtagagcag catcacggat gcaaacgagg
     2581 caagcagcag catatccgcc cgttgcggcg cattccagcc gttggcattg gcaaagaggg
     2641 cattgaaaac tgcgcctgca tcgtcggggt ttgcggtatt gaagacgacg aaggtcaggc
     2701 agacgaaatg gaaggtaaca aaccacgaga gcggcgcaag gtatttcaga cggcatagtg
     2761 cgttgcgtcc gaaatagcgg tcgcccgtgt tgagcagcac cagcgccgtg ccgtgcagcg
     2821 cgccccaaat gaggaagttc cagccgtagc cgtgccagat gcctgagagt accattgccg
     2881 ccatcaggtt gagctgtgtc cgtaaaaagc cttttttgct gccgcccaag gggatgtaga
     2941 tgtagtcgcg tatccaggtg gaaaggctga tgtgccattt gtcccaaaat gcacggatat
     3001 tcgcggcacg aagcggtgct gagaagtttt tgggcagtcg gaagcccagc agcattgcca
     3061 tgccgataac caaatcggaa tatccggaaa agtccaggaa gagttggaag gtatagccgt
     3121 acacgccgcc caatacgccc cagccgtcga attgggcggg gttttcaaat acgggcgata
     3181 cccagttttc cgccagcatc cccgccagcc accatttttt ggcaataccc agcagaatca
     3241 gggaaacggc gagtgcggga cggacgggcg aacgcggttg gcgggtacgg atttgcgcca
     3301 atgcgcccgc ctgttcgccg tccgcgcttt tgaatgcggc ggcgcggata atcgggccgg
     3361 aggtaacggt ggggaaaaaa ctcaggtgca gcagcagttc gtgccactcg aaacgcgcgg
     3421 cgtgcggcgc acggaagcag taaaccaaat aggcgattga ctggaaggtg taatacgaaa
     3481 gccccagcgg catcaggatg tcgattgcgc cgccttgtcc ggcatattgt gcaatcagcg
     3541 ggcggaaaaa gtcgaaatat ttgaaaaagc ccaagacggt cagcgagacg gcaatgccgc
     3601 accccagcca aaagcggcgc gtattttcgc gatcggaacg gagcagttcg cccaaaaggt
     3661 acacgcagga ggaataaagg acgacgattg ccgcaaatac ggggccgata tggtagagcc
     3721 agcccatacc ggcagccaaa agcagcaggt tttggacgga cgggtatttc gccaagcccc
     3781 agtaaatcgg caggaaggcg agaaagaaca gtgcgaactc gacagacagc agcggcataa
     3841 accatccttg gaataagaaa ataggcgtta aaacacggca aagcccgtta ttttaagcag
     3901 gggcggatat ttcttcaatc aaaatgccgt ctgaagggcg ggaggggctt cagacggcat
     3961 cgcgcggtca ggtttggata tggcggttca agttcaggca cagccacagc ccggccgccg
     4021 ccgcaagcat accggcgatg ccgatacggg aaatgccgac atattgcgtt acccaatgtc
     4081 ccaacagtgc gccgctgccg ataccgacgt t
//
LOCUS       DAWFLT010000121         4026 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWFLT010000121 DAWFLT010000000
VERSION     DAWFLT010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.121"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            156..1673
                     /locus_tag="WGR74_002031"
     CDS             156..1673
                     /locus_tag="WGR74_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0906364.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGVAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1734..3614)
                     /locus_tag="WGR74_002032"
     CDS             complement(1734..3614)
                     /locus_tag="WGR74_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ0906365.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQKGWQ
                     KGLHAVLFADYEFGLPLTGVDSERGGNLAMHWFADCADIDAASWLAQHSDGIPAGIST
                     PQSSVSETDYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDVEGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQTD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIQAQALPNTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLKPCAGGLGFEGIFNV
                     VIRTLSLKPVSASDGIVSGIGGPDSNAQARTAGQGGATPHPFDSNPPYRGVYGVGSGI
                     VIDSDPAAEYRECGWKARFLNELRPDFGIFETLRVENRRCALLDRHLCRLKTAAQALN
                     LPLPDGCENQIKQYIAHLPDGAFRIKALLASDGISLSRAVLNHLADKQRVIISPTILP
                     AQNYLRRFKTTHRALFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKYQGQWLT
                     PSLDLDILNGIMRQAVLDEPQKYLQTNQVIETHITQKTLQEAEEIRLSNALRGVFAAA
                     LA"
BASE COUNT          833 a          910 c         1317 g          966 t
ORIGIN      
        1 ctataaatgt gaaatccgcc ctttgaaaat cgggcggcgg ttttttgcct gcccgctttc
       61 agacggcaaa gccggttttc acgggtttct gcctgttttt cggatggttt gacgtgcttc
      121 ggcggcgtgt ttgccagaaa ggtaaatgac agggtatgtt gtatttcaga tacggttttt
      181 tggttgtttg gtgtgcggca ggtgtttctg ccgcctatgg ggcggatgcg cccgcgattt
      241 tggatgacaa ggcattgttg caggtgcagc ggtcggtgtc ggataagtgg gcggaatcgg
      301 attggaaagt tgaaaatgat gccccgcgcg tggttgacgg ggattttttg ttggcgcatc
      361 cgaaaatgtt ggaacatagt ttgcgcgacg cgctcaacgg caatcaggcg gatttaatcg
      421 cttcgttggc ggatttgtat gccaagctgc cggattatga cgcggttttg tacggcaggg
      481 cgcgggcttt gctggcgaaa ttggcgggaa ggccggcgga ggcggtggcg cggtatcggg
      541 aactgcacgg ggaaaatgcg gcagacgagc ggattttgct ggatttggcg gcggcggagt
      601 ttgacgattt ccggctgaag tcggcagaaa ggcattttgc ggaggcggca aaattggatt
      661 tgccggcgcc ggttttggaa aatgtggggc gttttcggaa aaaaacggag gggctgacgg
      721 gctggcgttt ttcgggcggc atcagtccgg cggtcaatag aaatgccaat aatgccgcgc
      781 cgcagtattg ccggcaaaac ggaggccggc agatatgcag tgtcagccgg gcggagcggg
      841 cggcaggctt gaattatgaa atcgaggcgg aaaaactgac ggcgttggca gataatcatt
      901 atttgttgtt ccgttccaat atcggcggca cgagctatta tttcagtaaa aaatcagctt
      961 atgacgacgg gttcggcagg gcgtatttgg gttggcagta taaaaatgca cggcagacgg
     1021 cggggatttt gccgttttat caggtgcagt tgtcgggcag cgacggcttt gatgcgaaaa
     1081 caaaacgggt aaacaaccgc cgcctgccgc cgtatatgct ggcgcacgga gtcggcgtgc
     1141 agttgtccca tacttaccgc ccaaacccgg gatggcaatt ttcggtcgcg ctggaacatt
     1201 accgccaacg ctaccgcgaa caggataggg cggaatacaa taacggcagg caggacgggt
     1261 tttatgtttc gtcggcaaaa cgtttgggcg aatcggcaac tgtgttcggc ggctggcagt
     1321 ttgtgcggtt tgtgccgaaa cgcgaaacgg tgggcggcgc ggtcaataat gccgcctacc
     1381 ggcgcaacgg tgtttatgcc ggctgggcgc aggagtggcg gcagttgggc ggtttgaaca
     1441 gtcgggtttc cgcgtcttat gcccgccgca actataaggg cgttgcggct ttctcgacag
     1501 aggcgcaacg caaccgcgaa tggaatgtct cgctggcttt gagccacgac aagttgtcgt
     1561 ataaaggtat cgtgcccgcg ttgaattatc gtttcggcag gacggaaagt aatgtgccgt
     1621 atgcgaaacg ccgcaacagc gaggtgtttg tgtcggcgga ttggcggttt tgaatggtgg
     1681 aataatgccg tccgaacttt gtggaaacag gttcggacgg catttttgcg cgttcaggca
     1741 agggcggcgg caaatacgcc gcgcaaggcg ttggagaggc ggatttcttc ggcttcttgc
     1801 agtgtttttt gtgtgatgtg tgtttcgatt acttgatttg tttgcaaata tttttgcggt
     1861 tcgtccaata cggcttggcg cattatgccg tttaaaatgt ctaaatctaa agacggtgtg
     1921 agccattgcc cttggtattt gacgaacacg ttgcttctgc cgccttcgag caggatgccg
     1981 tctgaattga aaaacaggct gtcgaacgcg ccttgtgttt cggcggtttg ccacgcttgg
     2041 tcgaagaggg cgcggtgggt ggttttgaag cggcgcaggt agttttgtgc gggcaggatg
     2101 gtcggcgaaa tgatgacgcg ctgtttgtca gccaaatggt ttaaaacggc gcgggacagg
     2161 ctgatgccgt ctgaagcgag cagggctttg atgcggaacg cgccgtcggg caagtgggcg
     2221 atgtattgtt tgatttgatt ttcgcagccg tcgggcaggg gcaggttgag ggcttgggcg
     2281 gcggttttca gacggcatag gtggcggtcg agcagggcgc atcgcctgtt ttccacgcgc
     2341 agggtttcaa aaatgccgaa atcggggcgc aattcgttga ggaaacgggc tttccagccg
     2401 cattcgcgat attcggcggc ggggtcgctg tcgatgacga tgccggaacc gacaccgtac
     2461 acgccccgat acggcggatt ggagtcaaac ggatgcggcg ttgccccgcc ttgccctgcc
     2521 gttcgggctt gcgcgtttga atccgggccg ccgatgcctg aaacaatgcc gtctgaagcc
     2581 gaaacgggtt tgagcgacaa ggtgcggatg acgacgttga atatgccttc aaaccccaac
     2641 ccgcccgcgc aaggtttcag gtagccgatg ctgcccgtat aaagcccacg cgcttcggtt
     2701 tcgagcgatt cgataatctg catactcatt tttttgggcg caccggtgat gctgccgcag
     2761 gggaaggcgg cgcggaggat gtcggcgaac gaggtgtttg gcagggcttg ggcttggatg
     2821 gtgctggtca tctgccagac gctgccgaaa cgcgatactt taaacggctc gggcacgcat
     2881 actttgccgg tttgggcgat tttgccgagg tcgttgcgca gcaaatcgac aatcatcacg
     2941 ttttcggcgc ggtttttcgg gtcggtttgc aactcggcgg cgcggcgttc gtcttgtccg
     3001 tcgcccaaaa tcggcgcagt gcctttcatc ggttcggtgc tgatggtgcc gtctgaagcg
     3061 atgttgagga agagttcggg cgagaaacac agcgtccacg cggattgccc ctccacatcg
     3121 ggcaggtggg acaagacggc ataggggacg ggctggcgca ggcggcggta gaggctgacg
     3181 ggattgccgt aggcttgcag gtgcaggcgg gtggtgtagt tgatttgata ggtgtcgccg
     3241 cgccggatgg cttcgtggat ttggcggatg tggtcgaggt aatcggtttc ggatacggag
     3301 gattgcggcg tggaaatgcc ggcggggatg ccgtctgaat gttgggcaag ccagcttgcg
     3361 gcatcgatgt cggcgcagtc ggcaaaccag tgcatggcaa gattgccgcc gcgttcggaa
     3421 tcaacccccg tcagcggcaa accgaattcg tagtctgcaa acaacacggc atgcagccct
     3481 ttttgccagc ccttttgcag cgcgccgtcc aaagcatcga gttcttcggg acggaaaaaa
     3541 cggctttcta catgattttg atagcgtttt gcgcggccgc ttacggcatc gtcaaacagg
     3601 gcgaaatagg gcatggcaat tcggggcaaa tgttttgatt atacgctctt tttacacata
     3661 ttttcagacg attcggcaaa tatcggcaaa atgtaatttt atgtagagaa agtggggggc
     3721 gaaggtgtaa aattgtgaaa acaaattccg atttccaacc tgaaatacaa taaggagacc
     3781 tttatgacag accaccagct gcaaccgttt gaaaacgtag aattaggcga aaagcaagac
     3841 cagctccaag tatagtggat taacaaaaac cagtacagcg ttgcctcgct ttagctcaaa
     3901 gagaacgatt ctctcaggtg ctgaagcacc aagtgaatcg gttccgtact atctgtactg
     3961 tcttcggctt cgttgtcttg tcctgatttt tgttaatccg ctataaagac cgtcgggcat
     4021 ctgcag
//
LOCUS       DAWFLT010000122         3909 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWFLT010000122 DAWFLT010000000
VERSION     DAWFLT010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.122"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(80..898)
                     /locus_tag="WGR74_002033"
     CDS             complement(80..898)
                     /locus_tag="WGR74_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0906366.1"
                     /translation="MKGMNKLRYQQDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDRPDPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIGNPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FAPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDIFRNFSL"
     gene            complement(978..2228)
                     /locus_tag="WGR74_002034"
     CDS             complement(978..2228)
                     /locus_tag="WGR74_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0906367.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAVRIWPS
                     HTYTRLTLESTAALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPDTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDADDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYLNTSVLKRG"
     gene            complement(2225..2686)
                     /gene="tsaE"
                     /locus_tag="WGR74_002035"
     CDS             complement(2225..2686)
                     /gene="tsaE"
                     /locus_tag="WGR74_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ0906368.1"
                     /translation="MSDLPSISRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYLLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGDGRKCLLTAHTERGRESLPL"
     gene            2888..3700
                     /locus_tag="WGR74_002036"
     CDS             2888..3700
                     /locus_tag="WGR74_002036"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ0906369.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAGNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT          867 a          942 c         1163 g          937 t
ORIGIN      
        1 ccgaaggtaa gggaggggtg tgtccactgt gccgccgcat ttgccgcgtg cggaagatac
       61 ggataacaac cccttggaat taaagggaga agttgcggaa aatatccata tcaggctgtg
      121 ggccgtactt atttacggta ttttcgtatg ccgaaataaa atatttcttg aataaaccgt
      181 aatcgccggt ttcgtaatag gctacgacag cctcggcata ggcgggataa ctgtcggggg
      241 cgaatacgca aggaaaaaaa ccggagcgca tcagcgacaa ggtcatacag tttctggcag
      301 tacgtttatt gcaatcttta aaatattgaa gatacgccaa attattatgg agataaacgg
      361 cgcggtcgaa gggatttcct atttttgggg cttcttgaag caaccatttt aattccgtat
      421 ccaaactttg cgggttggat aaaggcgtgt agtctgtccc gcttatagta acgctgtcgc
      481 ggcggactac gccgccggag cctttttcca gtaagttttc cgaaatcagg ctgtgggtgg
      541 tttttaagaa atccaaccag tcaaacggat cggggcggtc caaaccggac agcaggtgcc
      601 ggtaactttc cctcaggttg attaacatta cggcatccga atataacttc ccgcctgcgg
      661 tttgcccgag tttaagcaat gcctgcgtat cgtattgatt ataagtgttt ccttccaatt
      721 tggcagaact gtacacaaaa tcgataccga tttcttcaat attacgcacg gagtccgtta
      781 aaacagataa aggaagccga tcggacaatt cggtcaggta ttcttgttcg ccggctgtaa
      841 aaatggggcg gatatttaaa aaatcctgtt ggtatctcaa tttattcata cctttcatca
      901 taaaaacgcg gtttggggcg gaagtaggtt tatgcctgcc ttcatccgac aactatattg
      961 aaacaccgcc ttttcaatca accccgcttc aatacggatg tattgaggta gcgttggaca
     1021 cccgaggcaa tggattgggc gcactgccga cggaaggatt cgctgcccag cagcttctct
     1081 tcggcaggat tggacaggaa ggcggtttcg accaggatag acggcatatc gggcgcgcgc
     1141 aaaacggcga aattggcttc gtccaccctg cctttgtgca agtggttgag cctgcccaat
     1201 tcttcaagca ccagtttgcc gagtttgcgg ctgtcgcgca gcgtggcggt ttgggtcatg
     1261 tcgagcaggg cggtatcgac attgcggttg ccgctggtcg gtacgccgcc gaccgcgtcg
     1321 gcattatttt gggtttgttc caagaatttg gcggcagagc tggtcgcgcc tttggtgttc
     1381 agcatataaa cccccgttcc gcgcgcggag gggctggtga aagcgtcggc gtggatggag
     1441 acgaatacgt ccgcccgccg tgcgcgccct ttggcgacac gcacgcccaa tgggatgaac
     1501 acgtcttcgt tgcgcgtcat aaagacgttg taacccaagg cttccaattg ttttttggtt
     1561 tcgcgggcga tggagaggac gacgtgtttt tcctgcaaac cgcccgggct gacggcgccg
     1621 gggtcttcgc cgccgtgtcc ggggtcgatc ataatgacgg gtctgcgccc gtttttgccg
     1681 cgttcggttt gtgcgggagt gttttgagcg aggtcggctt caggggagcc gcgacgggtt
     1741 ttgttcaggc tgccgttgag cagcgccatc atcggatcgt cggcatccat cccttgcgga
     1801 tagagatcga cgacgaggcg gtttttaaag ccgccgacgg gtgggaggga aaaaacttgc
     1861 gcgtaggaag gctgtttcaa atcgatgacg aggcggacgg tgtcgggcgt gttctgaccg
     1921 gcgcgtatgc tgcggataaa ggggtcgtcc gccataactt tttgggaaag tccgtgcaat
     1981 acggtattga tgttcgcgtt ttgtatatcg acgaccagcc tgcccggatt gtcgagcgtg
     2041 aagtgttggt atttgagcgc ggcggtgctt tccagcgtca ggcgggtgta ggtgtgcgac
     2101 ggccatatcc gtacggcggt aaactgcggg gcgcgtaccg ttttggcaac ggcggatgcg
     2161 atggggctta gggcgaacag tgtgccggcg gtgcggcgga tgatttgtct tcgtgtcagt
     2221 ttggtcatag cggcaggctt tcgcgtcctc gttcggtatg ggcggtcagc aggcattttc
     2281 tgccgtcgcc gtcgtgtgtc aatgttgcgg tgatgtcggc aggcggggta aattccccac
     2341 cttgttgcgg ccattcgatc aggcagacgc tgtttgcggc aaacagttcg tccaatccgg
     2401 catcttccca ttcttcgggg aacgagaagc ggtagaggtc gaaatggtgc aaggtgaagc
     2461 gttccagcag ataagattcg acgatggcgt aagtcgggct tttgactgcg ccctgatgac
     2521 ccaatccgcg caggatgccg cgtgtcagcg tggttttgcc cgcacccaaa tccccctcga
     2581 gataaatgac cagcggcgcg tttaaacggg aagaccacgc cgcacccaaa tcgagtgtgg
     2641 cggcttcgtc ggcaaggaat cgggagatag agggtaaatc agacatggaa acggtttgtt
     2701 gtaaggtcta gggtattatg ggcagttttg caggttttgc aaactttgca cccgaggggc
     2761 ggatgcttct tgtccgagca ttataacagc caaatccgcg ttctgctttc agacggcaac
     2821 ggctgtcaag aaaaagcggc gcgtgtacaa tacgcggatt gtatgtttag gacagattgg
     2881 aaaaaggatg gaaaatatcg gcaggcagcg acccatcggc gtttttgact cgggaatcgg
     2941 cggtttgacc aatgtgcgcg cgctgatgga gcggctgccg atggagaaca tcatttattt
     3001 cggcgacacg gcgcgcgtgc cttacgggac gaaatctaag gcgaccatcg aaaatttctc
     3061 gatgcagatt gtcgattttc tgctggaaca cgatgtcaag gcgatggtta tcgcgtgcaa
     3121 cacgattgcg gcggtggcgg ggcagaaaat ccgtcaaaag acgggcaata tgcctgtgtt
     3181 ggacgtgatt tccgccggcg cgaaagccgc gctggcaacg acgcgcaaca ataaaatcgg
     3241 catcatcgcc accaatacca cagttaacag caatgcttat gcgcgcgcca tccacaggga
     3301 caaccccgac acgctcgtcc gcacgcaggc tgcgccgctg ctcgtccctt tggtggaaga
     3361 gggctggttg gaacacgaag ttacccgcct gaccgtatgc gaatacctca aaccattgct
     3421 tgcagacggc atcgatacgc tggtgttggg ctgcacgcac tttcccttgc tcaagccctt
     3481 aatcggcagg gaggcgggca atgtcgcgtt ggttgattcc gcgattacga ctgccgaaga
     3541 aaccgcacgc gtccttgcgc aggagggatt actcaatacc gacaacaaca atcccgacta
     3601 ccgtttttac gtcagcgata ttcctttgaa attcagaacc atcggcgagc gttttctggg
     3661 caggacgatg gagcagattg aaatggtgtc tttgggttaa aacgatgacg gaaagctgcc
     3721 cgagattaca gaaacctaaa atcccgtcat tcccacgaaa gtgggaatct agacctgtcg
     3781 gtgcggaaac ttatcggata aaacggtttc tttagatttt acgttctaga ttcccgcttt
     3841 cgcgggaatg acgggatgca ggtttctgtg cggatgtatt cggattccct cctgcgcggg
     3901 aatgatggg
//
LOCUS       DAWFLT010000123         3903 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWFLT010000123 DAWFLT010000000
VERSION     DAWFLT010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.123"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            456..3659
                     /gene="nalP"
                     /locus_tag="WGR74_002037"
     CDS             456..3659
                     /gene="nalP"
                     /locus_tag="WGR74_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
                     /protein_id="HEZ0906370.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGGTSAPDFNAGG
                     TGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPNLHT
                     GDFTNPNDAYKNLINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYNE
                     NYKNYTAYMRKEAPEDGGGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDVVSHIIG
                     GRSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFGTT
                     SRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNMLFI
                     FSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLSAP
                     YEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIGAV
                     GVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKGGS
                     QLQLHGNNTYTGKTIIEGGSLVLYGNNKSDMRVETKGALIYNGAASGGSLNSDGIVYL
                     ADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSARGKGAGYL
                     NRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRGNA
                     ARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMPGI
                     RPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLDHN
                     ATGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGHSTWSE
                     NSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGTLM
                     QLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGLAG
                     LKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVAGL
                     GADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1057 a         1056 c         1041 g          749 t
ORIGIN      
        1 tagattcccg cctgcgcggg aatgacggct gcagatgccc gacggtcttt atagcggatt
       61 aacaaaaatc aggacaaggc gacgaagccg cagacagtac agatagtacg gaaccgattc
      121 acttgttaaa gaatcgttct ctttgagcta aggcgaggca acgccgtact ggtttttgtt
      181 catccactat aactagggaa attcaaatta agttagaatt atccctatga gaaaaagccg
      241 tctaagccgg tataaacaaa ataaactcat tgagctattt gtcgaaagtt caaatttcca
      301 ttttaaaaca attagtaaaa tcgagtttat cctagttgtc caagacaacc cctataataa
      361 tataattcaa aatataaaaa cgggttacat ctaaacatta cggaattttt attccctcgc
      421 ctgaattcta ttgtcagatt caaggagacc tcatcatgcg aacgacccca accttcccta
      481 caaaaacttt caaaccggct gccatggcgt tagctgttgc aacaacactt tctgcctgct
      541 taggcggcgg cggcggcact tctgcgcccg acttcaatgc aggcggcacc ggtatcggca
      601 gcaacagcag ggcaacaaca gcgaaatcag cagcagtatc ttacgccggt atcaagaacg
      661 aaatgtgcaa agacagaagc atgctctgtg ccggtcggga tgacgttgcg gttacagaca
      721 gggatgccaa aatcaatgcc ccccccccga atctgcatac cggagacttt acaaacccaa
      781 atgacgcata caagaatttg atcaacctca aacctgcaat tgaagcaggc tatacaggac
      841 gcggggtaga ggtaggtatc gtcgatacag gcgaatccgt cggcagcata tcctttcccg
      901 aactgtatgg cagaaaagaa cacggctata acgaaaatta caaaaactat acggcgtata
      961 tgcggaagga agcgcctgaa gacggaggcg gtaaagacat tgaagcttct ttcgacgatg
     1021 aggccgttat agagactgaa gcaaagccga cggatatccg ccacgtaaaa gaaatcggac
     1081 acatcgatgt ggtctcccat attattggcg ggcgttccgt ggacggcaga cctgcaggcg
     1141 gtattgcgcc cgatgcgacg ctacacataa tgaatacgca tgatggaacc aagaacgaaa
     1201 taatgtctgc agccatccgc aatgcatggg tcaagctggg cgaacgtggc gtgcgcatcg
     1261 tcaataacag ttttggaaca acatcgaggg caggcactgc cgaccatttc caaatagcca
     1321 attcggagga gcagtaccgc caagcgttgc tcgcctattc cggcggtgat aaaacagacg
     1381 agggtatccg cctgatgcaa cagagcgatt acggcaactt gtcctaccac atccgtaata
     1441 aaaacatgct tttcattttt tcggcaagca atgacgcaca agctcagccc aacacactga
     1501 ccctattgcc attttatgaa aaagatgctc aaaaaggcat tatcacagtc gcaggcgtag
     1561 accgcagtgg agaaaagttc aatggctcca accattgcgg aattactgcc atgtggtgcc
     1621 tatcggcacc ctatgaagca agcgtccgtt tcacccgtac aaacccgatt caaattgccg
     1681 gaacatcctt ttccgcaccc atcgtaaccg gcacggcggc tctgctgctg cagaaatacc
     1741 cgtggatgag caacgacaac ctgcgtacca cgctgctgac aacggctcag gacatcggtg
     1801 cagtcggcgt ggacagcaag ttcggctggg gactgctgga tgcgggtaag gccatgaacg
     1861 gacccgcgtc ctttccgttc ggcgacttta ccgccgatac gaaaggtaca tccgatattg
     1921 cctactcctt ccgtaacgac atttcaggca cgggcggcct gatcaaaaaa ggcggcagcc
     1981 aactgcaact gcacggcaac aacacctata cgggcaaaac cattatcgaa ggcggttcgc
     2041 tggtgttgta cggcaacaac aaatcggata tgcgcgtcga aaccaaaggt gcgctgattt
     2101 ataacggggc ggcatccggc ggtagcctga acagcgacgg cattgtctat ctggcagata
     2161 ccgaccgatc cggcgcaaac gaaaccgtgc acatcaaagg cgatctgcag ctgggcggcg
     2221 aaggtacgct gtacacacgt ttgggcaaac tgctgaaagt ggacggtacg gcgatgaccg
     2281 gcggcaagct gtacatgtcg gcacgcggca aaggggcagg ctatctcaac cgtaccggac
     2341 aacgtgttcc cttcctgagt gccgccaaaa tcgggcggga ttattctttc ttcacaaaca
     2401 tcgaaaccga cggtggtctg ctggcttccc tcgacagcgt cgaaaaaaca gcgggcagtg
     2461 aaggcgacac gctgtcctat tatgtccgtc gcggcaatgc ggcacggact gcttcggcag
     2521 cggcacattc cgcgcccgcc ggtctgaaac acgccgtaga acagggcggc agcaatctgg
     2581 aaaacctgat ggtcgaactg gatgcctccg aatcatccgc aacacccgag acggttgaaa
     2641 ctgcggccgc cgaccgcaca gatatgccgg gcatccgccc ctacggcgca actttccgcg
     2701 cagcggcagc cgtacagcat gcgaatgccg ccgacggtgt acgcatcttc aacagtctcg
     2761 ccgctaccgt ctatgccgac agtaccgccg cccatgccga tatgcaggga cgccggctga
     2821 aagccgtatc ggacgggttg gaccacaacg ctacgggtct gcgcgtcatc gcgcaaaccc
     2881 aacaggacgg tggaacgtgg gaacagggcg gtgttgaagg caaaatgcgc ggcagtaccc
     2941 aaaccgtcgg cattgccgcg aaaaccggcg aaaatacgac agcagccgcc acactgggca
     3001 tgggacacag cacatggagc gaaaacagtg caaatgcaaa aaccgacagc attagtctgt
     3061 ttgcaggcat acggcacgat gcgggcgata tcggctatct caaaggcctg ttctcctacg
     3121 gacgctacaa aaacagcatc agccgcagca ccggtgcgga cgaacatgcg gaaggcagcg
     3181 tcaacggcac gctgatgcag ctgggcgcac tgggcggtgt caacgttccg tttgccgcaa
     3241 cgggagattt gacggtcgaa ggcggtctgc gctacgacct gctcaaacag gatgcattcg
     3301 ccgaaaaagg cagtgctttg ggctggagcg gcaacagcct cactgaaggc acactggtcg
     3361 gactcgcggg tctgaagctg tcgcaaccct tgagcgataa agccgtcctg tttgcaacgg
     3421 cgggcgtgga acgcgacctg aacggacgcg actacacggt aacgggcggc tttaccggcg
     3481 cgactgcagc aaccggcaag acgggggcac gcaatatgcc gcacacccgc ctggttgccg
     3541 gtctgggcgc ggatgtcgaa ttcggcaacg gctggaacgg cttggcacgt tacagctacg
     3601 ccggttccaa acagtacggc aaccacagcg gacgagtcgg cgtaggctac cggttctgac
     3661 ggacaggaag cagacagccg caaagatcac ggtctttgcg gctgtttctt atgaaaagaa
     3721 aaccctattc caattgcctg cttctattgt ttcaagactt cttccaaaga ttcggcatta
     3781 atcagatgta tagcggatta acaaaaatca ggacaaggcg gcgaagccgc ggacagtaca
     3841 aatagtacgg aaccgattca ctcggtgctt gagcacctta gagaatcgtt tctttgagct
     3901 aag
//
LOCUS       DAWFLT010000124         3862 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWFLT010000124 DAWFLT010000000
VERSION     DAWFLT010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.124"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="WGR74_002038"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="WGR74_002038"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ0906371.1"
                     /translation="MKEFDFIKRYLQTGTDNDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFANVKPEDLAWKVLAVNVSDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="WGR74_002039"
     CDS             1439..1924
                     /locus_tag="WGR74_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ0906372.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMLGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WGR74_002040"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WGR74_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ0906373.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGTKPKRIKYKPVTR"
BASE COUNT          783 a          941 c         1080 g         1058 t
ORIGIN      
        1 aaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cttcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 ttgccagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataat
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgcaaa tgtcaaacct
      661 gaagacttgg cttggaaggt tttggccgtc aatgtttcgg atatggcggc gatgggtgcg
      721 ataccgcgtt gggtgttgct gagcgcggcg ttgcccgaat tggatgaggt atggctgaaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcgttgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgttgg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgt ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgtgcctt cttcgccgag
     2041 tcggcgtttc ttgtcggctt cgtattcctg atagttgccg tcaaagaaca cccatttgga
     2101 gtcgccttcg caagccaaga tatgcgtagc aatacggtcg aggaaccagc ggtcgtgcga
     2161 aatcaccatc acgctgccgg caaattccag caatgcgtct tccaacgcgc gcagggtttc
     2221 cacgtcgagg tcgttggacg gttcgtccag cagcaacaca ttgccaccgc ccaacaaggt
     2281 ttttgccaag tgcaaacgtc cgcgttcgcc gccggaaagc tgccccgtga ttttgctttg
     2341 gtcgctgcct ttgaaattga agcgtcccaa atattggcgg gcggggattt caaactgccc
     2401 gacctgtaaa atatcgcgac cttcggcaat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgcccgattt tcacttcacc
     2521 ggaatcgggc tgctctttgc ccgcaatcat tttaaacagt gtcgatttac ccgcgccgtt
     2581 cggaccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttgtcg ccgaacgatt tggaaacatt cacaaattca atcacttcgt tacccaaacg
     2701 ctcggcgacg ggaatgaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcttca aaacgagcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgtgttacc gcaacgactg taccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 agattccgcg tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gtttgctcaa
     3121 caagagtttg cacaaggcaa cgcggcgttt ttcaccgccg gacaaattat cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc ggcgatttcc aattcgtgtt ccgcaccgcc
     3241 gcccgtggac gaacctgccg caataatcgc ttccaagcgg ccctgctctt ctgccaacgc
     3301 gtcaaaatcc gcatcaggat tggcgtactc ggcatacact tcttccaaac gtttctgcgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcgcgc acggtttttt caggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt aatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacacccg ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcagg ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgctta
     3781 tccctttgaa attatataga caaaaaaata ataatgatag gggatcgccg ccccggcaac
     3841 catttcggat tttccaaagc aa
//
LOCUS       DAWFLT010000125         3844 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWFLT010000125 DAWFLT010000000
VERSION     DAWFLT010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.125"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(94..531)
                     /gene="rnhA"
                     /locus_tag="WGR74_002041"
     CDS             complement(94..531)
                     /gene="rnhA"
                     /locus_tag="WGR74_002041"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ0906374.1"
                     /translation="MNQTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDALVGRHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            645..1514
                     /gene="tehB"
                     /locus_tag="WGR74_002042"
     CDS             645..1514
                     /gene="tehB"
                     /locus_tag="WGR74_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEZ0906375.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKAENLPEILLSGYSSEGGEWVCL
                     NVLQGDVEVRAPDGSAEVWSADGGDCVFAPQQVFSVKPKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAAQDTVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            complement(1564..2370)
                     /gene="thiD"
                     /locus_tag="WGR74_002043"
     CDS             complement(1564..2370)
                     /gene="thiD"
                     /locus_tag="WGR74_002043"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ0906376.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSSSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVRTAKSYITAAISSPLEIGAGHGPVNHWAYRD"
     gene            2431..2628
                     /locus_tag="WGR74_002044"
     CDS             2431..2628
                     /locus_tag="WGR74_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906377.1"
                     /translation="MPSENRSAGVCGALYAVLQDKAQGRAAGCIVATVFLPDGVKNTV
                     ATPTDNEKREQCRLNRVLRRA"
     gene            complement(3672..3747)
                     /locus_tag="WGR74_002045"
     tRNA            complement(3672..3747)
                     /locus_tag="WGR74_002045"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3711..3713),aa:Glu,seq:ttc)
BASE COUNT          855 a          928 c         1113 g          948 t
ORIGIN      
        1 tctggattcc cgcctgcgcg ggaatgacgg tggaggacga tgccgtctga agcccattag
       61 cggtttcaga cggtattttt gtcggtgcgg cagtcaggaa aactgcgccg cgccacggtt
      121 tgccaaatcg tcggcgcgtt cgttttccgc gtgtcccgcg tgtcctttca cccaagtcca
      181 gctgacttgg tgccgtccga ccagcgcgtc gagttctttc cacaagtcgt cgtttttgac
      241 gggctgtttg gcggcggttt tccagccgtt gcgcttccaa ccgtgtatcc agttttccat
      301 gccgtttttg acgtattgcg agtcggtgca gatgatgacg gtgcagcggc gtttgagcga
      361 tttcagtcct tcgatgacgg cggtcagttc catacggttg ttggtggttt gcgcttcgcc
      421 gccgaaaagt tctttttcgt ggctgccgta gcgcattaat acgccccagc cgcccgcgcc
      481 gggattgcct ttgcacgcgc cgtcggtgta aaggtaaacg gtttggttca ttattgtgcc
      541 tttatgtggt gtccggcgtg attataagcg gtttgtgcgc cgtgccgtct gaaagattgc
      601 ggcggattaa aataggcata taatgtgcaa ctttattttt gaatatgaag gagcggatag
      661 tgggtcaaag cggcgagttg ttttgtttcg gacaaatgcc cgtatggaag gcggaaaacc
      721 tgccggaaat tttgttgtcg ggttattcgt ccgaaggagg ggaatgggtc tgcctgaatg
      781 tgttgcaggg cgatgtcgaa gttcgtgcac cggacgggtc ggcagaagtt tggtcggcag
      841 acggcggcga ttgtgtgttt gcgccgcagc aggtgttttc tgtcaaaccg aaaacggacg
      901 atgccgaaat ccgtttgtcg ctgtattgcg cggcggcaga ctattttcac aaaaaatacg
      961 ggatgagtgc cacgcattct gcggtcgcgg cggcacagga taccgtaccg gcgggcaggg
     1021 cgttggatat gggctgcgga caggggcgca acgcgctgtt tcttggtttg aaggggtttg
     1081 aagttactgc agtcgatcac aatcccgccg ccttggcaaa cgtggcggag ctggcagagg
     1141 cggagggttt gaacgtccgc acgctggaat atgatttgaa cgccgccgcc ttgcagggtg
     1201 agtttgatta tattgtggca acagtggtgc tgatgttcct gatgccgcag cgcgtgcctg
     1261 acgtgattgc cgatatgcag gcgcatacgg cggcgggcgg gtacaactta atcgtatcgg
     1321 caatggatac ggcggatttc ccttgtccga tgccgttccc ttttaaattt aaagagggag
     1381 agctgaagga ttattatcgg gattgggagc tggtcgaata taaagaagaa ttgggcgcga
     1441 tgcacgccaa agacgaaaac ggcaatccga tacggtttaa atttgtaacg atgctggcga
     1501 aaaagcctga ataatcctca aaaatgccgt ctgaacattt tgtttcagac ggcattttta
     1561 cggttagtcc cgataggccc aatgattgac cgggccgtgt cccgcgccga tttccaaagg
     1621 gcttgagatt gccgccgtga tgtaggactt ggcagtccgt acggctttgc aaacgtctaa
     1681 gccttttgcc aactcggcgg taatgcaggc agaaaacgtg cagcccgtgc cgtgcgtgtg
     1741 ggcggtcgga aagcgcgggc tgtccaattc cagcgtttcg ttttgcgtaa acagccaatc
     1801 cgtgcagcgt ccgctgctgc tgccattcaa atgtccgcct ttgatgacga catttttgac
     1861 accgtaatca agcaggattt ttgccgcacg ttccgcatct ttacggtttt cgatatgcac
     1921 gccggtcagg gcttccgcct cgggcaggtt gggggtcaat acatccgtat cgggaagcag
     1981 caggcgcgtc agtgccgcaa cggcggaatc ctgcaacagc ggcgcaccgc ctttggcaat
     2041 catcacaggg tcgagtacgc gcctgccgaa gtcgcagtgt ttcagcttgt cggcaacgca
     2101 ttcgatgatt tccgccgtgc cgagcattcc tattttgtag gcgcggatgt caaagtcttc
     2161 cctgattgct tggatttgtg cggtgatggt ttcggtcggg acgagatgaa ccgccgacac
     2221 gcccaaggta ttttgcgcgg taacggcggt gatgacgcac gttccgaaca cgccgcgcat
     2281 ctgaaacgtt ttcaaatccg cctgaatgcc cgcaccgccg cccgaatccg aaccggcgat
     2341 ggtaagcgtt tgcacaaaag agcttttcat ttgttcctcc ttaatattgt gtccgataaa
     2401 aagggctaat gcctgtaaaa aagcacggcg atgccgtctg aaaaccgcag cgcgggggtt
     2461 tgtggtgcat tatatgccgt attgcaggac aaggcacagg gcagggcggc ggggtgcatc
     2521 gtagcaactg tatttttgcc cgacggggtg aaaaatacag ttgctacacc cactgataat
     2581 gaaaaaagag agcaatgccg tctgaaccgc gtgttgcggc gagcataaac cggcacgctt
     2641 tattcgtcat ttccacaaaa acagaaacct aaaattccgt cattcctacg aaagcgggaa
     2701 tccagtgtgt tcggtttcag ttgtttttgg gtttcgggtg atttccaaat catcattccc
     2761 gcgcaggcgg gaatctagcc cttggggcag cggcaatatt tataaagccg tctgaaaatt
     2821 cagaagttct agattcccgt tttcacggga atgacgaagg gttgtgggaa tgacgaagtt
     2881 tcagacggca tcgccccgcc ccgtcattcc cgcataagcg ggaatccaaa ccattgggca
     2941 gagcagcctt aaaggttata aaagactcgt cattcccacg aaagtgggaa tccagacctg
     3001 tcggcacgga aacttatcgg ataaaacggt ttcttcaacc ctacgctcta gattcccgct
     3061 tttgcgggaa tgacgaagtt tcagacggca tcaaatgctt tgtcaggctt cagacagcat
     3121 cgtccaccct ctgccgtcct tcccgcgcag gcgggaatcc aatgtgttcg gtttcggttg
     3181 tttttgggtt tcgggtaatt tccaaatcgt cattcccacg aaagtgggaa tccagaccat
     3241 tgggcggcgg aaatattcaa aagtcgtctg aaaattcaga ggttctagat tcccgttttc
     3301 acgggaatga cgaagggttg tgggaatgac gaagttttag acggcattga atactttgat
     3361 gctttggtgg gctgaagccc accctacaat atagagaaaa tgccgccaaa cggtatggaa
     3421 gcggaaacaa tggtggggct ggcttcagaa acaatggtag ggtgggcttc agcccaccgc
     3481 tttagacggc atccgcgcac cgtcattccc gcgcaggcgg gaatccagcc ttgtcggcac
     3541 ggaaacttat cggataaaac ggtttcttca accctacgct ctagattccc gttttcacgg
     3601 gaatgacgaa gagtgtgaga atgacggttc ataaataaaa aaacctgctt aaaacaagca
     3661 ggtttttgaa ttggcacgcc cacggggatt cgaaccccgg ttgccgccgt gaaagggcag
     3721 tgtcctaggc ctctagacga tgggcgcgtg ggaatgggca ttatacatac ttctagaaat
     3781 ttagcaatac gatgccgtct gaaacttcgt cattcccgcg aaagcgggaa tctagaatct
     3841 ccaa
//
LOCUS       DAWFLT010000126         3795 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWFLT010000126 DAWFLT010000000
VERSION     DAWFLT010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.126"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(370..1410)
                     /gene="queA"
                     /locus_tag="WGR74_002046"
     CDS             complement(370..1410)
                     /gene="queA"
                     /locus_tag="WGR74_002046"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ0906378.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVTVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGWNEGVVR"
     gene            1527..1784
                     /locus_tag="WGR74_002047"
     CDS             1527..1784
                     /locus_tag="WGR74_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ0906379.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPKHRQPLKEQKWICAN"
     gene            1766..2221
                     /locus_tag="WGR74_002048"
     CDS             1766..2221
                     /locus_tag="WGR74_002048"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ0906380.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKVGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            2333..3694
                     /gene="accC"
                     /locus_tag="WGR74_002049"
     CDS             2333..3694
                     /gene="accC"
                     /locus_tag="WGR74_002049"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ0906381.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIEAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIASGLPLQYKQKDIKIEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFNDPGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
BASE COUNT          924 a         1152 c          921 g          798 t
ORIGIN      
        1 aatttatctt ataaacaaac aaaggcagcc tgaaagattg aatggtctgc acccctaagg
       61 ttggactaac caaccgacta aggtgcagat tattttttgt tgctttttca gcttttcgtt
      121 gggttagata ttcttgccca ctgttttcag gcagccttga atacaaaaaa tggcgtatgt
      181 aatatgttta tacgaccaaa acggaatgaa ttttaacgta ttgcgcgtca tcaacaatga
      241 ctgagtttct cgcctctcgc gcctgaatct atgtcatata cagttccttt cattcaaatc
      301 aacaaaagaa tgccgtccga acgtccgttc agacggcact tgtcttcccg caatagactt
      361 ggggctgttc taacgtacca ccccttcgtt ccaccccaaa accatcgcat cgccgtagct
      421 gaagaaacgg tattcgcgtt cgaccgcata acgatacgcg gcgcggatat gacccatacc
      481 cgaaaacgcg ctgacaagca tcagcagcgt cgatttcggc aaatgaaaat tggtaaccag
      541 cctgtcgaca acattaaaac ggtagcccgg cgtgatgaaa atatcggtgt cgccctgccc
      601 cgctttcaga cgacccgtcg cacgcgcggc agattcgagg gcgcgcatgg aagtcgtgcc
      661 gactgcccag actttgttcc cccgggcttt tgccgcctca acggcggcgg cggtttcaga
      721 cggcacttca aaccattcgc tgtgcatttt atgctcttcg attttgtcca cacgcacggg
      781 ttggaatgtc cccgcaccga cgtgtagggt tacttctgcg gttactgcgc ctttgtcttt
      841 caggcggcgc aaaagttctt ccgtaaaatg cagacccgcc gtcggcgcgg cgaccgcgcc
      901 ctgatatttg gcataaacgg tttgataacg gctgtcgtca tccgcatcgg cggcgcgttc
      961 gatataaggc ggcaggggca ggtgtccgtt ctgttccaaa agttcgtaaa cggtttgacc
     1021 gccttcaaaa cgcaggcaga acagttcgcc ctcacgcccg accgtcacgg cgcggatgcc
     1081 gccttcaaac accagcccca taccgggctt gggcgatttg gacgaacgga tgtgcgccag
     1141 tgcggtatgg ttgtccaaca cgcgctcaat cagggcttcg atcctgccgc cgctgtcttt
     1201 ctgcccaaac agccgcgcct tcatgacttt ggtgttgttg aacaccaaaa cgtcgcccgc
     1261 ctcaacataa tccggcaaat cgccgaacac ccggtcttgc agcggcatat cgggcaacgc
     1321 aaccaaaagg cggctgctgc cgcgcacttc gggcggatgc tgggcaatca gcttttcggg
     1381 cagggtaaaa tcaaaatctg aaatatccat ttttacactc tcgttcgggc aagccgccat
     1441 tatacgcact ttagcccttt ttcagacggc atctttgtcc gaaaaaccaa cagattagaa
     1501 taaacactct taacccgggg catcttgtgc gcaaaatcaa acttcctgca catttccccc
     1561 aaaaaccgcc gttttttgat attttactgg acatttaccg acaacttcgg gaaaataaac
     1621 acattctcac ggtcgttttc caccacagga aaaccgtatc cgaacaccat tccgcccggt
     1681 ttgcgccgtt gccgcaagcc ggctgttttc tgaaaaacca acgcaacaac ccgccgaaac
     1741 accggcagcc tttaaaggaa cagaaatgga tttgcgcaaa ttaaaaaaac tgattgattt
     1801 ggttgaagaa tcgggtatcg ccgaaatcga agtaaccgaa ggcgaggaaa aagtccgcat
     1861 cacccgaacc atcgccgccg cacccgttta cgccgcgccc gtacctgccg ccgcgccggc
     1921 cgtaacgcct gccgccgcac ccgttgcggc atccgcgcct gccgccgcac ctgccgcccg
     1981 cgatttgtcc gacgcgcaaa aatcacctat ggtcggcacg ttctaccgcg cacccggccc
     2041 gaatgccgcg ccttttgtcg aagtcggcca acaggttaaa gtcggcgaca cgctgtgcat
     2101 catcgaagcg atgaagctga tgaacgaaat cgaagccgaa aaatccggca cggtcaaaga
     2161 aattttggtc gaaaacggta cgcccgtcga attcggcgaa ccgctcttca tcatcggata
     2221 atcctgtttt cagacggcat aaacttccga tgccgtctga aatgctttcc cccttcagcg
     2281 ttcccgcacc cttttttacg gacgggttgc cggaaccgca ggaaaggtca tcatgctgaa
     2341 aaaagtttta atcgccaacc gaggcgaaat cgcattacgc gtactccgtg cctgccgcga
     2401 aatgggcatt gccaccgtcg ccgtgcattc cgaggccgac aaagacagcc tgcacgtcaa
     2461 actcgccgac gaatccgtgt gcatcggccc tgccgcttcc gcgcaaagct acctcaacgt
     2521 ccccgccatt atcgccgccg ccgaagtaag ctgcgcggac gctgtccacc cgggctacgg
     2581 tttccttgcc gaaaacgccg atttcgccga acaggtcgag cagtccggct ttacctttat
     2641 cggcccgaaa cccgacacca tccgcctgat gggcgacaaa gtatccgcca aacacgccat
     2701 gatcgaagcg ggcgtgcctt gtgttcccgg ctctgacggc gcattgcccg acgacggcga
     2761 agaaatcctc aaaatcgccg ataaagtcgg ttatcctgtc attatcaaag cctctggcgg
     2821 cggcggcggt cgcggtatgc gcgtggtcga gaaaaaagaa gacctcctcc aatctgtcga
     2881 aatgaccaag gcagaagcag gcgcggcatt cggcaacccg atggtttaca tggaacgcta
     2941 tttgcaacgt ccgcgccacg tcgaaatcca agtgattgcc gacgaacacg gcaacgccgt
     3001 ctaccttgcc gaacgcgact gttcgctaca acgccgccac caaaaagtca tcgaggaagc
     3061 accggctccg ttcatcactg aaaaagaacg cgccaaaatc ggcaacgcct gtgccgacgc
     3121 ctgcaaacgc atcggctatc gtggcgcagg tacgtttgaa ttcctgtacg aagacggcga
     3181 atttttcttt atcgagatga atacgcgcgt tcaggttgag catccggtta ccgagctcat
     3241 caccggcgtg gacattgtgc aggagcaact ccgcatcgca tccggcctgc cgctgcaata
     3301 caagcagaaa gacatcaaaa tcgaaggcca cgcgtttgag tgccgtatca acgccgaaga
     3361 cccatacaac ttcatcccca gcccgggccc gattgaaagc tgccacctgc ccggcggctt
     3421 cggtatccgc gtcgacagcc acatttacca aggctaccgc atcccgcctt actacgacag
     3481 cctgatcggc aaaatctgcg tacacggcaa aacgcgtgaa caggcaatgg cgaaaatgcg
     3541 cgtcgcactc gccgagctgg cggtaaccgg catcaaaacc aatacgccgc ttcaccgcga
     3601 cttgttcaac gacccaggtt tccaaaaagg cggcgtcagc atccactatt tggaacactg
     3661 gctggaagat cgcaaagcca aacaggacaa ataagccgcc gccgatatgc cgtctgaaac
     3721 agtttgtccg ttttcagacg gcatttccct tgccccgcgc cgtctgaaac cgatttcgat
     3781 atagtggatt aactt
//
LOCUS       DAWFLT010000127         3709 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWFLT010000127 DAWFLT010000000
VERSION     DAWFLT010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.127"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            270..1391
                     /gene="dnaJ"
                     /locus_tag="WGR74_002050"
     CDS             270..1391
                     /gene="dnaJ"
                     /locus_tag="WGR74_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ0906382.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1586..3604
                     /locus_tag="WGR74_002051"
     CDS             1586..3604
                     /locus_tag="WGR74_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ0906383.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGK"
BASE COUNT          777 a          830 c         1115 g          987 t
ORIGIN      
        1 aatagggggg aagtctgcag tcgcatcaaa tgccgtctga acatgcgttc gggcggcgtt
       61 tttataacaa aaacacttca tggcggttgg ttttatgcct gtctaagttt ttgtgtcgtg
      121 catacctgaa gatttcagac ggcatcggtt tatgctgtct gaaaagtgta ttccgtttca
      181 gtttgtaagc tatggcagtc tgtttgtctt gtgttttgcg caattgccct tattttgagc
      241 cgtgatttta ttttgaatta gatgaaaaaa tgagtaatca agatttttat gcgacgctgg
      301 gtgtggcaag aacagctacc gatgatgaga ttaaaaaagc ctaccggaaa ttggcgatga
      361 aataccatcc cgaccgcaat cctgacaata aagaggcgga agagaagttt aaagaagtac
      421 aaaaggcgta tgaaactttg tccgacaagg aaaagcgcgc tatgtacgac cagtatggtc
      481 atgcggcgtt tgaaggcggc ggacaggggg gcttcggagg gtttggcgga tttggcggtg
      541 cgcagggttt tgactttggg gatattttca gccaaatgtt tggaggcggt tcggggcgcg
      601 cccagcctga ttatcagggt gaggacgttc aagtcggtat cgaaatcacg cttgaagaag
      661 ccgcaaaagg tgtgaagaaa cgcatcaata ttccgactta tgaagcgtgt gatgtctgta
      721 acggcagtgg cgcgaaaccg gggacatccc cggaaacctg cccgacttgc aaaggttcgg
      781 gtacggtgca catccagcag gcgattttcc gtatgcagca gacttgtccg acctgccacg
      841 gtgcgggcaa acacattaaa gaaccttgcg tcaaatgccg tggcgcgggg cggaataagg
      901 cggtcaagac ggtggaagtc aatattcccg ccggtatcga tgacgggcag cgtatccgtt
      961 tgagcggcga aggcggaccg ggtatgcacg gtgcgcctgc tggcgacctg tatgtaaccg
     1021 tccgcattcg ggcgcataag attttccaac gcgacggtct ggacttgcat tgcgaactgc
     1081 cgatcagttt tgccacggct gctttgggcg gggagttgga agtgccgacc ttggacggaa
     1141 aggtcaagct caccgtcccc aaagaaaccc aaaccggcag gaggatgcgc gtgaagggta
     1201 agggtgtcaa atctttacgc agcagcgcga ccggcgattt gtactgccat attgttgtcg
     1261 aaacgcctgt caatttgacc gaccgtcaaa aagagctttt ggaagaattt gagcggattt
     1321 ctaccggctt ggaaaaccaa acaccgcgca agaaatcgtt tttagacaag ctgcgcgatt
     1381 tgtttgattg attttaaggt tcggaaacaa gcagccgtat cggggaatct ccctgatacg
     1441 gctgttttgt ttgtttaaaa atagttttta ttttcaatgg ggtatggggc agggtgggat
     1501 aactgttttt aactgttctt tttaaaactt gacatcatgg cgcgatgcca acaatatgtg
     1561 aacgtctgtt gtcaaaggaa gaataatgaa taaatcttta tccgggtcgg tagaagaata
     1621 ccgcgaactg acgctccgag gcatgatact cggtgcattg atcactgtaa tttttactgc
     1681 gtccaacgtt tacctcggtt tgaaagtcgg gctgaccttt gcctcgtcga ttccggcggc
     1741 ggtaatttcg atggcggttt taaagttttt caaaggcagc aatattttgg aaaacaacat
     1801 ggtgcagacc caagcttcgg cggcgggtac gctttcgacc atcatcttcg tcctgcccgg
     1861 tttgctgatg gcgggctact ggagcggttt cccgttctgg cagacgacgc ttttatgtat
     1921 tgccggcggg attttggggg tgattttcac cattccgctg cgttacgcga tggtggtgaa
     1981 aagcgatttg ccttatccgg aaggtgtggc ggctgctgaa attttgaaag tgggcggtca
     2041 tgaagaaggg gataaccgtc agggcggcag cggcatcaaa gagctggcgg ccggcggtgc
     2101 gttggcggga ttgatgagct tttgcgccgg aggtctgcgc gtgattgccg acagcgcgag
     2161 ttattggttt aaaagcggta cggcgatttt ccagctgccg atgggctttt cactggcatt
     2221 gttgggcgcg ggctatttgg tcggactgac gggcggtatc gccatcctgt tgggcatttc
     2281 gattgcttgg ggcattgccg tgccgtattt ctcctcacac attccgcaac cttccgatat
     2341 ggaaatggcg gcgtttgcga tgaagctgtg gaaggagaaa gtgcgtttta tcggtgcggg
     2401 gactattggc attgcggcgg tttggacgct gttgatgctg ctcaagccga tggtggaagg
     2461 catgaagatg tcgttcaaga gttttggcgg cggtgcgccc gctgcggaac gcgccgaaca
     2521 agatttgtcg cctaaagcca tgattttttg ggtgctggcg atgatgtttg ttttaggcgt
     2581 gtcgttttac cactttatcg gcgattcgca cattacgggc ggcatggctt ggcttttggt
     2641 ggtcgtttgc acgcttttgg cttccgtcat cggctttttg gtcgccgccg cctgcggtta
     2701 tatggcaggt ttggtcggct cgtcttccag cccgatttcc ggcgtgggca tcgtgtccgt
     2761 cgtcgttatt tcactggttt tgctgctggt aggcgaatcc ggaggtttgt tggcggatga
     2821 ggctaaccgc aaatttttgc tggcactgac tttgttttgc ggctcggcag taatctgcgt
     2881 ggcttcgatt tccaatgaca acctgcaaga cttgaaaacc ggctacctgc tcaaagccac
     2941 gccttggcgg cagcaagtcg ccctgattat cggctgtatc gttggtgcgc tggttatttc
     3001 gcccgtgttg gaactgcttt acgaagccta cggctttacc ggcgcaatgc cgcgcgaagg
     3061 catggatgcg gcgcaggctt tggcagctcc tcaagcgact ttgatgacga ccatcgcgtc
     3121 gggcattttc gcccacaacc tcgaatgggt ctacatcttt accggcatcg cgatcggcgc
     3181 ggtattaatc gtcgtcgatt tggtgttgaa aaaatcatca ggcggcaaac ttgccctgcc
     3241 cgtccttgcg gtcggtatgg gtatttatct gccgccgtcc gtcaatatgc ccatcgtggc
     3301 aggcgcggtg ttggcggcgg tgttgaaaca catcatcggt aaaaaagcgg aaaaccgcga
     3361 aggccgtctg aaaaacgccg agcgtatcgg aaccttgttc tccgccggcc tgattgtcgg
     3421 tgaaagcctg atcggtgtga ttatggcgtt tattattgcc ttctccgtga ccaacggcgg
     3481 ctcggatgcg ccgcttgcgt tgaatctgca aaactgggat gccgccgctt cttggttggg
     3541 tttggcgttc ttcgttaccg ggatgttttt ctttgcacag cgcgtactga aggcgggcaa
     3601 gtaggctgtc ggaaaaaatg ccgtctgaag cgttcagacg gcatttttat ctggaaagcg
     3661 gaaggcggag cttttcggct tgcgcccacg ttttgccggc aaggtcttt
//
LOCUS       DAWFLT010000128         3584 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWFLT010000128 DAWFLT010000000
VERSION     DAWFLT010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.128"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            35..922
                     /gene="prmA"
                     /locus_tag="WGR74_002052"
     CDS             35..922
                     /gene="prmA"
                     /locus_tag="WGR74_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ0906384.1"
                     /translation="MPYQQITINVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQIAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEEGWARLSGTKR"
     gene            940..1503
                     /gene="orn"
                     /locus_tag="WGR74_002053"
     CDS             940..1503
                     /gene="orn"
                     /locus_tag="WGR74_002053"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ0906385.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDELLDNMDEWNTATHGRTGLTQRVRESLHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPSVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(1909..3192)
                     /gene="hemL"
                     /locus_tag="WGR74_002054"
     CDS             complement(1909..3192)
                     /gene="hemL"
                     /locus_tag="WGR74_002054"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ0906386.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FRTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIDAFKRFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(3256..3429)
                     /locus_tag="WGR74_002055"
     CDS             complement(3256..3429)
                     /locus_tag="WGR74_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ0906387.1"
                     /translation="MKSERRHWEWCVDYDWEGVVRDYRFTHKVEKDERSVIRDAVGVI
                     RKEAGKSLSQPEK"
BASE COUNT          881 a         1057 c          927 g          719 t
ORIGIN      
        1 aattcaatcc actatatttc caagaaagcc cgttatgccc taccaacaaa tcaccatcaa
       61 cgtcaacgat gccgtcgccg aacgcctcgc cgacgcgctg atggaacacg gcgcactctc
      121 cgccgccatc gaagatgcct acgccggcac gcaaaacgaa caggcgattt tcggcgaacc
      181 cggtatgccc gccgaacaaa tctggcagca gagcaaagtc atcgccctgt tcggcgaaca
      241 cgacgaagcc gccgccatca tccaaatcgc cgcacaagaa tgcgggttaa aagacttggc
      301 atacaccggc gaaaccatcg aagaccaaga ctgggtgcgt ctcacgcaat cgcaattcga
      361 ccccatccga atttccgacc gcctgtggat taccccctct tggcacgaag cccccgaagg
      421 cactgccgtc aacctacgcc tcgatcccgg cctcgccttc ggcactggca gccacccaac
      481 cacgcgcctc tgcctcaaat ggctggatac gcaactcaaa aacggcgaaa gcgtcctcga
      541 ctacggctgc ggttcgggca tcctgaccat cgctgccctc aaactcggcg caggttctgc
      601 cgtcggcgtc gatattgacg aacaagccgt ccgcgccggc aaggacaacg ccgaacaaaa
      661 caacgtcgat gcacaattct tcctgcccga cggcctgcct caagggcaat tcgacgtagt
      721 tgtcgccaac atcctcgcca accctttacg tatgctcggc gaaatgctcg ccgcccgcac
      781 caaacaaggc ggccgcatcg tgttatccgg tttgttggac gaacaagccg aagaactcgg
      841 cggcatttac agccaatggt tcgatctcga cccggcggaa accgaagaag gctgggcgcg
      901 cttgagcggg acaaaacgct gaaacggaaa ggaaacaccg tgcaggacaa aaacaacctc
      961 tgctggctcg atatggaaat gacggggctt aatcccgaaa ccgaccgcat tatcgaagtc
     1021 gcgatgatta ttaccgactc ggatttgaat gtattggcgc aatccgaagt ttacgccgtc
     1081 caccaaagcg acgagctgct cgacaatatg gacgaatgga acaccgccac acacggtagg
     1141 acggggctga cacagcgcgt gcgcgaatcg ttgcataccg aagccgaagt cgaacagaaa
     1201 ctgctggact ttatgtcgga atgggtaccc ggacgcgcca cgccgatgtg cggcaactcc
     1261 atccaccaag accggcgttt tatggtcaaa tatatgccga aactggaaaa ctacttccac
     1321 taccgcaacc tcgacgtttc cacgctcaaa gagcttgcca aacgctggaa tccgtccgtt
     1381 gccaaaagcg tcgtcaaacg cggttcgcac aaggcattgg acgacatttt ggagagcatc
     1441 gaagaaatgc gccactaccg cgaacacttt ctgatttccg ccccgagagc cgaagcgcaa
     1501 taagaaacaa aaaatgccgt ctgaagcgca aatcctgcat ttcagacggc atttttacag
     1561 cagattgaaa tcaaaaatat acacgcccgt cattcccgcg caggcgggaa tccggaaggt
     1621 cgggcctgcc gttattttca atcattacag aaactgaaag gtctggattc ccgcctgcgc
     1681 gggaatgacg ggcgtgtgca ttcttatagt ggattaacaa aaatcaggac aaggcgacga
     1741 agccgcaaac agtacaaata gtacgaaacc gattcacttg gtgcttcagc accttagaga
     1801 atcgttctct ttgagctaag gcgaggcaac gccgtactgg tttttgttaa tccactatac
     1861 ttcaatctgc caaacagatc gaacagagaa actctgtccg tcaaaacatc atgcagccat
     1921 cgccttgaac acttcaaccg caaccgcaac cgtttcgtca atcagctcgg gcgtatgcgc
     1981 ggcagaaacg aaacctgctt cataagcgga cgggccgaag gcaatgccgc ggtcgagcat
     2041 accgtggaag aagcgtttga aagcgtcaat attggaacgc gccatatcgg cataatttcg
     2101 cggcgcgtgt gcggcgaaat acagaccgaa cataccgccc acgctgtcgg cggtgaactc
     2161 gatgcccgcc gcatccgctg ccgtccgaaa accttgaacc aactgttcgg tacgcgccgt
     2221 caggttttca taaaaccctt cgcggcggat gatttccagc gttttcaaac cggcggcgac
     2281 ggcaatcggg ttgcccgaca aggtgcctgc ctgatacacg ccgcccagcg gggaaataca
     2341 ttccataatg tctttgcgtc cgccgaacgc ggcaagtggc ataccgccgc cgatgacttt
     2401 gcccatcgtg gtcaggtcgg gcgtgatgcc gtgcagcgat tgtgcgccgc cgagcgcgac
     2461 gcggaaaccg gtcatcactt cgtcgtaaat caacactgcg ccgtgttttt cggtcaattc
     2521 gcgcagggct ttgacgaagg cttcggtcgg gcggacaagg ttcatattgc cgacaaaagg
     2581 ctccaaaatc acacaggcaa tgtcattgcc gctttgagca aaggcttctt ccaattgggc
     2641 gatgttgttg tactcaagga ctaaagtgtg tttggtaaag tcggcaggca caccggcgga
     2701 agaaggattg ccgaacgtca gcaggccgct gccggctttc accagcaggc tgtcggaatg
     2761 cccgtggtag cagccttcaa acttgatgat tttgtcgcga ccggtaaaac cgcgtgccaa
     2821 acggatggcg gtcatcgtcg cttcggtacc ggagctgacg aggcgcagcc gttcgacgga
     2881 cggcatgatt ttggcgattt gttcggcaat ggcgatttcg ccctcggtgg gcgcgccgaa
     2941 cgacaaaccg cccaacgcgg cttcgcgcac ggcttcgacg acttcggggt gcgcgtgtcc
     3001 gacaatcgca ggcccccaag agccgacata gtcggtataa cgcgtgccgt tttcgtccca
     3061 aacatacgcg ccttcggctt ttttgatgaa gcgcggcacg ccgccgacgc tgccgaatgc
     3121 gcggacgggg gaattcacgc cgccggggat gatggctttg gcgcggtcga ataaaatttc
     3181 gttacggttc atatttatcc tcaaatgccg tctgaacggc aggtttcggg cttagaagca
     3241 gaaagtccca ttttatcatt tttcaggttg cgacaaggat ttgcctgctt ctttgcggat
     3301 gacgccaacc gcatcccgga tgacggaacg ctcgtctttt tccactttat gtgtaaagcg
     3361 gtagtcccgg acaactcctt cccagtcgta atccacacac cactcccaat gccggcgttc
     3421 tgatttcata taaatgaaat tggtcggcaa aaaattataa atcggcaggc tgacttcatg
     3481 ataggcataa caaccgaaag ggttgcgctt cccgaaacgt gcctctacct ccgcccgggt
     3541 cgttttgcct ttaacaaccg tttgtgcgat tccctcttcc gtct
//
LOCUS       DAWFLT010000129         3457 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWFLT010000129 DAWFLT010000000
VERSION     DAWFLT010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.129"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            208..1680
                     /gene="pyk"
                     /locus_tag="WGR74_002056"
     CDS             208..1680
                     /gene="pyk"
                     /locus_tag="WGR74_002056"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ0906388.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRTGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQVEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            1990..3384
                     /locus_tag="WGR74_002057"
     CDS             1990..3384
                     /locus_tag="WGR74_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ0906389.1"
                     /translation="MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNERHSFGAGIIAQHTSAELRKYADWGIMEKAKALKETPPNPTKAAQIKADGHA
                     DVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADDAMAKQLWDANK
                     LALLGYTPSEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFEKE
                     KTIVNGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMNSL
                     PDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARFKN
                     HADIIGLQYTYKFK"
BASE COUNT          897 a         1058 c          867 g          635 t
ORIGIN      
        1 aaataaattt agcctaagtc ataaaaccgt gtaattttgc gccaaaaaag gcggtttgca
       61 ggtaaaatat caaactatta atttaaatca tatttttaca gaatattccg ccgcgttcat
      121 caaatggaca tcaaaccggt tccaaatttc tgtaattttg taacaaaata ccgcaaaaca
      181 cccgattgag accaaaagga ctttcatatg aaccagacaa gccgcgatct gacccgcatc
      241 agccacaaca ctaaaatcgt cgccaccctt gggccgggca gcaacaacgt cgaactgttg
      301 gaagacatga tccgcgtcgg cggtctgaac gtcgtccgct tcaacttcag ccacggcacg
      361 cccgaattcc atcaggaaaa cgcccgcatc gtgcgcgagg cggcaaaacg caccggacag
      421 gaaatcgcca tcattgccga cctgcagggc ccgaaaatcc gcgtgggcaa aatcgccggc
      481 ggcggcatcg aattgaacaa aggcgaaacg ctggtactgg atgccgcgct cgaaggcgaa
      541 ggcacgcgcg aggcggtcgg tttggactac cgcgacctgc ccgacgacgt tgccgcaggc
      601 gatgtcttgt ggctggacga cggcctgctg accctgaccg tggaatccgt cgaaggcagc
      661 aggattatca caagggtgga aaacagccac gtcctgaaaa gcaacaaggg catcaacaaa
      721 cgcggcggcg gtctgtccgc aggcgcgttg accgaaaaag acttccgcga cctgaaaacc
      781 gcgattgcca tcggttgcga ctatctcgcc atcagctttg tgaaatccgc cgaagatttg
      841 cacatcgcgc gcgccaaagt cgaagaagaa atgaaaggca gtacggccgt gcgccccggt
      901 ttggtttcca aaatcgaacg cgtagaagcg attgaaaacc ttgatgaaat catcctcgcc
      961 ggcgacggca ttatggttgc acgcggcgac ttggcggtcg aagtcggaca cgccgccgtc
     1021 cccgccctgc aaaaacggat gatccgccgc gcccgcgagt tgcgccgctt cagcattacg
     1081 gcgacgcaaa tgatggaatc gatgattacc aaccccgtgc cgacccgcgc ggaagtcagc
     1141 gatgtggcaa acgcggtatt ggacggcacc gatgcggtga tgtgttccgc cgaaaccgcc
     1201 gtcggcgcgt atccgtttga aaccgtcagc caaatggcga ttatctgcgc ggctgcggaa
     1261 aaagagcagg attcgctcaa cggcgttgcc gaacaggtcg agtatcccga agcggtcagc
     1321 accaacctgg cggttgccgg cggtgcggtc agcgtggcgc gcgcggttca cgccaaagcc
     1381 atcgtcgccc tgaccgaaag cggttcgacc gcctttgaaa tcagccgcca caacatcacc
     1441 ctgccgattt tcgcgctgac cccgagcgtt tccgcccaac gccgtatggc gatgtaccgg
     1501 ggcgtgcgcc cgctgatttt ggcaaccagc accgaccacg acacggcgtt aaacgaagtc
     1561 gaaaccatgc tggtggaaca caacatcctg cattccggcg accaatacat cattaccagc
     1621 ggctcgcaaa tgcgcgaatc cggttcgacc aatacgctgg aagtgctgcg cgtcaaataa
     1681 tccgccctga gtggaaaatg ccgtctgaag tcgatgcccg aggcttcaga cggcattttt
     1741 tgcggcggcg cggcggttcg ggcaaactct gaaaaaattg tgcaaatccg gcaacatcgg
     1801 ataaaatcga gtacctatac taaagcgaaa caaggcattt ccgactgcct tttttatagt
     1861 ggattaaatt taaaccagta cggcgttgcc ttgccgtact atctgtactg tctgcggctt
     1921 cgtcgccttg tcctgatttt tgttaatcca ctatatttat ccaccgtccg cctttttacg
     1981 gaaaccgaaa tgaccccttc cgcactgaaa aaaaccgtcc tactgctcgg cactgccttt
     2041 gccgccgcat ccgcacaagc ctccggctac cacttcggca cacagtcggt caacgcgcaa
     2101 agcacggcaa atgccgccgc cgcagaagcc gccgacgcat cgaccatctt ctacaaccct
     2161 gccggcctga ccaaactcga cagcagccag atttccgtca acgccaacat cgtgctgccc
     2221 agcattcatt atgaggcgga ttccgccacc gactttaccg ggcttcccgt ccaaggttcg
     2281 aaaagcggca aaatcaccaa aaccacggtc gcgccccaca tctacggcgc atacaaagtc
     2341 aacgacaatc tgaccgtagg cttgggcgtg tacgtcccct tcggttctgc caccgaatac
     2401 gaaaaagatt ccgtgttgcg ccacaacatc aacaaactcg gtctgaccag catcgccgtc
     2461 gaacctgtcg ccgcgtggaa actcaacgaa cgccattcct tcggcgcagg catcatcgcc
     2521 caacatactt ccgccgagct gcgcaaatat gccgactggg ggattatgga aaaagcgaaa
     2581 gcactaaaag aaacaccccc caatccaact aaagccgccc aaatcaaagc cgacggacac
     2641 gccgatgtca aaggcagcga ttggggcttc ggctaccaac tggcgtggat gtgggacatc
     2701 aacgaccgtg cgcgcgtggg cgtgaactac cgttccaaag tctcacacac gctcaaaggc
     2761 gatgccgaat gggcggcaga cgacgcaatg gcgaaacagt tatgggatgc aaacaaactc
     2821 gcactgctcg gctacacgcc aagcgaaaaa gcccgcgtta aaatcgttac gcccgagtct
     2881 ttgtccgtac acggtatgta caaagtgtcc gacaaagccg acctgttcgg cgacgtaact
     2941 tggacgcgcc acagccgctt cgataaggcg gaactggttt ttgaaaaaga aaaaaccatc
     3001 gtcaacggca aatccgaccg caccaccatc acccccaact ggcgcaacac ctacaaagtc
     3061 ggcttcggcg gttcttatca aatcagcgaa ccgctgcaac tgcgcgccgg catcgctttt
     3121 gacaaatcgc ccgtccgcaa cgccgactac cgcatgaaca gcctgcccga cggcaaccgc
     3181 atctggttct ccgccggcat gaaataccat atcggcaaaa accacgtcgt cgatgccgcc
     3241 tacacccaca tccacatcaa cgacaccagc taccgcacgg cgaaggcaag cggcaacgat
     3301 gtggacagca aaggcgcgtc ttccgcacgt ttcaaaaacc acgccgacat catcggcctg
     3361 caatacacct acaaattcaa ataaacggcc cgccgtttga ttgtagaaat gccgtctgaa
     3421 acagatttcc gtttcagacg gcattgtttt aggcgcg
//
LOCUS       DAWFLT010000130         3412 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWFLT010000130 DAWFLT010000000
VERSION     DAWFLT010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.130"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            53..721
                     /locus_tag="WGR74_002058"
     CDS             53..721
                     /locus_tag="WGR74_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ0906390.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AEQVANLLRFSRGEAVRLNLPVYICPVQVKKGGAPNNKCDFGKKGQGMLAFGDKNGNK
                     TYDGDTKDVFLRNVVLNDDINDKRINYTFNHIAFGSSQPTADRVVWTFNQNGTFGYTK
                     DRHLTERSSFSYSDGYIQIVLTDARAVSDADKKFRSAVVLINSSGRVEVCPRGDRRTM
                     CQYK"
     gene            745..1368
                     /gene="pilV"
                     /locus_tag="WGR74_002059"
     CDS             745..1368
                     /gene="pilV"
                     /locus_tag="WGR74_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ0906391.1"
                     /translation="MKNNDCFRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNNTLSHTLSAVDGEFA
                     IDAIKTKTQLAEAQLKRFSYELKNALPDAAAIHYAVCKDSSGAAPTLSAGGAFSSNCD
                     ASANGDTLIKVLWVNDSAGDSDIARTNLETNGNNIVYTYQARVGGRE"
     gene            1365..2306
                     /locus_tag="WGR74_002060"
     CDS             1365..2306
                     /locus_tag="WGR74_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ0906392.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGLLSMIVLMAVGSSYFT
                     SRKLNDAANERLAAQQDLRNAATLIVRDARMAGGFGCFNMSEHTTTDVVSDTTQQNSP
                     FSLKRNGIDKLIPIAESLNINYQNFFQVDSALIFQYGIDDVNASTATTVVSSCAAISK
                     PGKQIPTLEDAKKELKIPDQDKEQNGNIARQRHVVNAYAVGRIADEEGLFRFQLDDKG
                     KWGNPQLLVKKVRHMKVRYIYVSGCPEDDDAGKEETFKYTDKFDSSTNAVTPAGVEVL
                     LSSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            2285..2881
                     /locus_tag="WGR74_002061"
     CDS             2285..2881
                     /locus_tag="WGR74_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ0906393.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCAKGLCTAVNVRT
                     NNTGNEEAFDNIVVQGNPTVEAVKRSCPANSTDLCIDKKGMKYKKGTRSVSKMPRYII
                     EYLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2886..3359
                     /locus_tag="WGR74_002062"
     CDS             2886..3359
                     /locus_tag="WGR74_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ0906394.1"
                     /translation="MEQKGFTLIEVMIVVTILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQTIKSKLEIFVSGYKMNPKIAEKYNVSVHFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASARAYSETLSADAGCEAFSNRKK"
BASE COUNT          965 a          691 c          865 g          891 t
ORIGIN      
        1 aacgccgtac tggtttgaat ttaatccacg atacattacc agttaacgtc ctatgtgtac
       61 acgaaaacaa caaggtttca cgctaacaga gctgctcatc gtgatggtca ttgcagccat
      121 tatggcgatg atagccctcc ccaatatgag ccaatggatt gcatcccgcc gcattgccag
      181 tcacgcggag caggttgcca accttttgcg tttctccagg ggcgaagccg tccggctcaa
      241 tctccctgtc tatatctgtc ctgttcaagt taaaaaaggc ggtgcgccca acaataaatg
      301 tgacttcggc aagaaggggc agggaatgtt ggcttttggc gataagaatg gcaataagac
      361 atatgacggc gatactaagg atgtttttct ccgcaacgtg gtattgaatg atgatatcaa
      421 tgataagcgg attaattaca ccttcaacca tatcgctttc ggttcgtctc agccgaccgc
      481 tgaccgtgta gtttggacgt tcaatcaaaa cgggacgttc ggttatacga aagaccggca
      541 tcttacagag cgatccagct tttcttattc cgacggttat atccaaatcg tgctgacaga
      601 tgcgagagca gtttcagatg ccgataagaa attccgttcg gcggtggttt tgattaacag
      661 cagcggcagg gtcgaagttt gccctagggg tgatcggcgt actatgtgcc agtataaata
      721 acagtttcag ttttaaaaat gaatatgaag aataatgatt gcttccgcct gaaaaatccc
      781 cagtccggta tggcgctgat agaagtcttg gttgccatgc tcgttctgac catcggtatt
      841 ttggcactat tgtccgtaca gttgcggaca gtcgcttccg tcagggaggc ggagacgcaa
      901 accatcgtca gccaaatcac gcaaaacctg atggaaggaa tgttgatgaa tccgaccatt
      961 gattcggaca gcaacaagaa aaactataat ctttacatgg gaaacaatac actatcgcat
     1021 acactatcag ctgtggatgg tgagtttgcg attgatgcca taaaaactaa gacgcagttg
     1081 gcagaggcac aattgaagag atttagttat gagctgaaaa atgccttgcc ggatgcggca
     1141 gccatccatt acgccgtctg caaggattcg tcgggtgctg cgccgacatt gtccgccggc
     1201 ggtgcttttt cttcaaattg cgatgctagt gcaaatgggg atactttgat taaagtattg
     1261 tgggtaaatg attcggcagg ggattcggat atcgcccgta cgaatcttga gacgaacggc
     1321 aacaatatcg tatataccta tcaggcaagg gtcggaggtc gggaatgaga cgtaaaatgc
     1381 taaacgtacc aaaaggcagt tatgatggta tgaagggttt taccattatt gaatttttgg
     1441 ttgcgggcct gctcagtatg attgtcctga tggcggtcgg atcgagttac ttcacatccc
     1501 ggaaattaaa tgatgcggca aacgagcgtc ttgccgcaca acaggatttg cggaatgcgg
     1561 caacattgat tgtccgcgat gcaagaatgg cagggggctt cggttgtttc aatatgtccg
     1621 agcatactac aactgatgtt gtttccgata cgacgcaaca aaattctcct ttttccttaa
     1681 aaaggaacgg tatagataaa cttattccca tagcggaatc tttaaatatc aattatcaga
     1741 attttttcca ggttgatagc gcattgattt ttcaatacgg aatcgatgat gttaatgcaa
     1801 gcaccgcgac taccgtcgtc agcagctgtg ccgcaatatc gaaaccgggc aagcaaatcc
     1861 ctactttaga agatgcaaaa aaagaattga agattccgga tcaggataag gagcaaaatg
     1921 gcaatatagc gcgtcaaagg catgtggtca atgcctatgc ggtcggcagg attgccgatg
     1981 aggaaggttt gttccgcttc caattggatg ataagggcaa gtggggtaat cctcagttgc
     2041 tcgtgaaaaa ggttagacat atgaaagtgc ggtatatcta tgtttccggc tgtcctgaag
     2101 atgacgatgc cggcaaagag gaaacattca aatatacgga taaattcgac agctccacaa
     2161 atgctgttac gcccgccggg gtggaggttt tattgagtag cggtactgat accaagattg
     2221 ccgcttcttc agacaatcat atttatgctt accgtatcga tgcgacaata cgcgggggaa
     2281 atgtatgcgc aaacagaaca ctttgacggg aatcccgact tctgacggac agagggggtt
     2341 cgcgctgttt atcgtactga tggtgatgat cgtcgtggct tttttggttg taactgccgc
     2401 gcagtcttac aataccgagc agcggatcag tgccaacgaa tcagacagga aattggcttt
     2461 gtctttggcc gaggcggctt tgcgggaagg cgaacttcag gttttggatt tggaatatga
     2521 tacggacagt aaggttacat ttagcgaaaa ctgtgcaaaa ggtctgtgta ccgcagtgaa
     2581 tgtgcggaca aataatactg gtaatgaaga ggcttttgac aatatcgtgg tgcaaggcaa
     2641 tcccaccgtt gaggcggtga agcgttcttg ccctgcaaat tctaccgacc tgtgcattga
     2701 caagaaaggg atgaaatata agaaaggcac gagaagcgtc agcaaaatgc cacgttatat
     2761 tatcgaatat ttgggcgtga agaacggaga aaatgtttat cgggttactg ccaaggcttg
     2821 gggtaagaat gccaataccg tggtcgtcct tcaatcttat gtaagcaata atgatgagta
     2881 ataaaatgga acaaaaaggg tttacattga ttgaggtaat gatagttgtc acgatactcg
     2941 gcattatcag cgtcattgcc ataccttctt atcaaagtta tattgaaaaa ggctatcagt
     3001 cccagcttta tacggagatg gtcggtatca acaatatttc caaacagttt attttgaaaa
     3061 atcccctgga cgataatcag accatcaaga gcaaactgga aatatttgtc tcaggctata
     3121 agatgaatcc gaaaattgcc gaaaaatata atgtttcggt gcattttgtc aataaggaaa
     3181 aaccaagggc atacagcttg gtcggcgttc caaagacggg gacgggttat actttgtcgg
     3241 tatggatgaa cagcgtgggc gacggataca aatgccgtga tgccgcttct gcccgagcct
     3301 attcggagac tttgtccgcg gatgccggct gtgaagcttt ctctaatcgt aaaaaatagg
     3361 gctgttttgc caatgccgtc tgaaaatcaa tgttcagacg gcatttttat gg
//
LOCUS       DAWFLT010000131         3378 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWFLT010000131 DAWFLT010000000
VERSION     DAWFLT010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.131"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(72..2243)
                     /locus_tag="WGR74_002063"
     CDS             complement(72..2243)
                     /locus_tag="WGR74_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0906395.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTSFGDGDHRIVLSHMKDQHRGIRTVREEFTVGDKSSRINIGR
                     QAPAYRETTQSNTNLAYTGKNLGFVEKLDANAYVLEKERYSADDSRTGYAGNVKGPNH
                     TQITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNGKFKISDKKPDPNDPTKE
                     IDKDAAEKAKDKKDMDLVHSYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVK
                     THDGKTVSSSNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIAD
                     GTKAERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHDSVAVREAVNAGYIK
                     NHGYELGASYRTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYR
                     FQNPNLEIGWRGRYVQKAVGSILVAGQKDRSGKLENVVRQGFGVNDVFANWKPLGKDT
                     LNVNLSVNNVFNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          752 a          777 c          957 g          892 t
ORIGIN      
        1 ctatatatca aattatcaga acagatgccg tctgaaaggc tttcagacgg cattttttcg
       61 ggatgtgcgt tttagaactt gtagttcacg cccaagcgta catcgcggcc cacgcccggc
      121 agggtattgg tccagcgttg gctgtgcgga tagtagaacg tgttgaacac gttgttaacc
      181 gaaagattaa cattgagcgt gtctttgccc agcggtttcc agttggcgaa gacatcgttc
      241 acaccgaaac cttggcgtac aacgttttcc aatttgccgc tgcggtcttt ttgacctgcc
      301 accaatatcg aacccacggc tttttgaaca tagcgtccgc gccagccgat ttccagattc
      361 ggattttgga agcggtaggc aagggaggcc gtccaagtgc ggccgacttg tgcgccaaat
      421 tcaggattcg cgctcaacag cttgtctttg tgcgtatcgt aaaagcgcgg tttgctgtgg
      481 cttacgccga ctttggcagt caggccgccg gtgcggtagg acgcgcccaa ttcgtaaccg
      541 tggtttttga tgtaaccggc gttgacggct tcacggacgg cgacagagtc gtggcggttt
      601 tgcggattgg caagcgcgtc tttgatggtt tgccagaagt agctgccgtt ggcggcaaac
      661 gtgccgtcgt tgtagttgaa gccgatttcg gtattgcgcg cgcgttcggc tttcgtgccg
      721 tcggcaatcg agatgatgcc gcgtttaccg tgggtttgca gcgcgtcata caggcgcggg
      781 ctgcggctgg cgtagttgtg gctcgcgctg aagctccagt gttcgtgcgg ctgccaaatc
      841 acaccgaaac tcgggttaag gttgctgctt gaaacggttt tgccgtcgtg ggttttcact
      901 ttgaagcggt cgtaacgcag cccgccggtc agggtaaagc cgtcaatctc gtgaatggct
      961 tcgatatacg cgccggtatc ggttttggtc ggattggaca gtttgtagga atggacaaga
     1021 tccatatctt tcttgtcttt cgctttttct gcagcgtcct tatctatttc ttttgtggga
     1081 tcgtttgggt ctggtttctt atcggagatc ttaaatttgc cgttcaaaaa cgcttgcggt
     1141 ttgatttcct gatggcggta gttgataccg tatttcagca gggtttgttc ggcaaggcgg
     1201 ctgtcgaagt tgaagttcat accccgagtg gtgatttggg tatggttggg gccttttaca
     1261 ttgcctgcgt agccggtgcg gctgtcatcg gcggaatagc gttctttttc caacacatag
     1321 gcgttggcat ccagtttttc gacaaagccc aggtttttac ccgtgtacgc caagttggtg
     1381 ttggattggg tagtttcgcg gtaagcaggg gcttggcggc caatatttat ccgtgaactt
     1441 ttgtcgccga cggtaaattc ttcacgcaca gtgcggatgc ctcggtgttg gtctttcata
     1501 tggctcaaca cgatgcggtg gtcgccgtcg ccgaagcttg ttccgatttt ggcgaggtag
     1561 ctgcgtttgt ccagcgcgct gtacggtacg gttttgccgc cgttaacatt gcggaaacct
     1621 ttgccggctt cgtaatcttt ttcatcgttg cggttgtaag agaacaaacc gtcgaagttg
     1681 ccctcttttc cgaatacgct tgcgccgtag cttacgcctt cgttgctggc aaagccgctg
     1741 ttgaggcgca cgccccagtt tttatccaag cctttgagca ggtcttgggc atcgacggtt
     1801 ttggcgataa tcgcgccgtt ggtcgcgccg ataccggcag aggcggaacc cgcgcctttt
     1861 tgtacggaaa cgactttaac caaagcggga tcgacaataa atctgccttg gtggtaaagg
     1921 atttggctgt cggaataggc gttgtccacc ttgatgtcga cagagttctg acccataccg
     1981 cgcagcgtca ggaattggga cgtgccgttg ccgccgccga aatcgatgga cggctcttct
     2041 tttaagagtt cgcgcatatc ggttgcggtg ctttcgtctt tttgttgaag cgtaacgatg
     2101 ttggtacgga ttttgctgcc ttggcggtcg ccttttacgg taacggtatc caatgcgaca
     2161 ttggcattat tttctgccgc atgggcaaaa cctgccgcca gggtaagcga gagcaggctg
     2221 aggcggaaaa acggggcgtt catttgttcg tccttttgag tgtatgaagg gaagtaaatc
     2281 caaaccgtta aggtttggca ggataagaaa aataataatt atttttgttt atattaacga
     2341 gaggggggat ttgtgtaaag ctgattatcg tttttatttg cgaagtgttg ttttttgttg
     2401 acaggttttg tcggaaatgt atagtggact aacaaaaatc aggataaggc gacgaagccg
     2461 cagacagtac aaatagtacg gcaagacgag gcaacgctgt actggttttt gttagtccac
     2521 tataaaaaac ggcggaaata tagtggatta acaaatgcgg gaatgacgaa aagcaacctt
     2581 tccgcgtcct tcccgcgaaa gcgggaatct agaaccccta acgcggcagg aatctatcgg
     2641 aaatgactga aaccgaacag actggattcc cgcctgcgcg ggaatgacga agcctgtgcg
     2701 ggaatgacga ggcctgcgcg ggaatgacga aaagcaacct ttccgcgtcc ttcccgcgaa
     2761 agcgggaatc tagaacccct aacgcggcag gaatctatcg gaaaaaaccg aaaccgaaca
     2821 gactggattc ccgcctgcgc gggaatgacg aggcctgcac gggaatgacg aagcctgcgc
     2881 gggaatgacg aaaagcaacc tttccgcgtc cttcccgcaa aagtgggaat ctaggaactc
     2941 aaagctgcaa gaatttatca gaaacaactg gaactcaaaa aaactagatt cccgcctgcg
     3001 cgggaatgac gaagggttaa agaaatgatg agaaacggcg gtaatttatc gggaataacc
     3061 gaaaacgaac ggaaatcagg acaaggcggc gaagccgcag acagtacgga taggacggaa
     3121 ccgattcact tggtgcttga gcaccttaga gaatcgttct ctttgagcta aggcgaggca
     3181 acgacgtact ggtttttgtt catccactat aacagcaacc ctgtcgccgt cattcccgcg
     3241 aaagcgggaa tccagtccgt tcagtttcgg tcatttccga taaattcctg ctgcttttca
     3301 tttctagatt cccacttttg cgggaatgac ggcggaaggg ttttggtttt ttccgataaa
     3361 ttcttgaggc attgaaat
//
LOCUS       DAWFLT010000132         3289 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWFLT010000132 DAWFLT010000000
VERSION     DAWFLT010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.132"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..258
                     /locus_tag="WGR74_002064"
     CDS             <1..258
                     /locus_tag="WGR74_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0906396.1"
                     /translation="HQLQDGIMRKACRNRPLTEAQTKRNRYLSKTRYVVEQSFGTLHR
                     KFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            658..990
                     /locus_tag="WGR74_002065"
                     /pseudo
     CDS             658..990
                     /locus_tag="WGR74_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
     gene            complement(1207..2001)
                     /locus_tag="WGR74_002066"
     CDS             complement(1207..2001)
                     /locus_tag="WGR74_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ0906397.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSCRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVEWIRENQ"
     gene            2045..2149
                     /locus_tag="WGR74_002067"
                     /pseudo
     CDS             2045..2149
                     /locus_tag="WGR74_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            2210..2935
                     /locus_tag="WGR74_002068"
     CDS             2210..2935
                     /locus_tag="WGR74_002068"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ0906398.1"
                     /translation="MPRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLSFLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          869 a          982 c          771 g          667 t
ORIGIN      
        1 catcagttgc aggacggcat tatgcgcaaa gcctgccgca accgtccgct gacggaagcg
       61 caaaccaaac gcaaccggta tttatcgaag acccgttatg tggtcgaaca aagcttcggt
      121 acgctgcacc gcaaattccg ctatgcgcgg gcagcctatt tcggactgat taaagtgagt
      181 gcgcaaagcc atctgaaggc gatgtgtttg aacctgttga aagccgccaa caggctaagt
      241 gcgcccgctg ccgcctaaaa ggcagcccgg atgcctgatt atcgggtatc cggggaggat
      301 taagggggta tttgggtaga attaggaggt atttggggcg aaaacagccg aaaacctgtg
      361 tttgggtttc ggctgtcggg agggaaagga attttgcaaa ggtctcgggc tatgccggct
      421 gctgccgccc attgcacctt cgccgaatcc tgccgcccac cgccgaagcc ctcatgcgtt
      481 cgcggtacag cgcgtatgtg ctactatagt ggattaacaa aaaccagtac agcgttgcct
      541 cgccttagct caaagagaac gattctctaa ggtgctgaag caccgagtga atcggttccg
      601 tactatttgt actgtcttcg gcttcgtcgc cttgtcctga tttttgttaa tccactatac
      661 atcatcgcta ctaccgttcc ggcacaacaa acattcctcg atgccgccga actgatgcag
      721 tggagcagag aaaccgaatg gctgggcttg aatgtcatcg cacaccgcaa cttcggcaaa
      781 cagcacgccc aagtcgaatt tgaagcctac ttccgagacg gacaacaccg atccgcgcat
      841 cacgaactgt ccggcttcat caacatcggc ggacaatggt attttatcga tcctaccgtt
      901 ccgcatcctg cgatgaaaca accctgcatt tgcggatcgg gcaaaaaatt caaagcctgc
      961 tgcggcaaat atctgaaacc tgtcgcataa aaatgccgtc tgaacgttca gacggcattt
     1021 tcaacacgca aaaaaaccat tcataccaag ggtaagtatg aatggtcaat acattgcggg
     1081 aaaacgtctt acttgctgca ctgccgaaaa gggagaaacg gcagcggtaa tcagcggaaa
     1141 ggattgtacc cgaattaatg ttaagaaacg ttaatcgtga aaatatatta acaaacctgt
     1201 tgaaacctat tggttttccc gtatccactc gacccagcgt tcaaacagct tcggttcgag
     1261 cgcggcgacg accgagcgtt tgaacacgtg ttcaccactc caaaacccgt cgccttccaa
     1321 agtcgtcagc ctgccgcccg cctcctcgaa aatcaacgcg ccggcggcat aatcccacag
     1381 cttctgcccg ccgtggacat aaacatcata acgcccgcac gccagataac accagtccaa
     1441 cgtactgctg cccatactcc gtatcgttcc aaaaggcgcg agcgtactca tgcggctgga
     1501 cagtttgccc gaacgcaaat atttgatttc cacgcccgca atcgcctcat tgagtttttt
     1561 atccacgaga cgcaaaggca ggcgggttcc attaagaaac gcaccctgcc cccgttcggc
     1621 ataaaaacat tcgccgctga ccgggttgta gattacgccc aattccgcac gcccgttgcg
     1681 gacaaacgcc accgacaccg caaaatgcgg cagcccgttg acaaaattgt tcgtcccgtc
     1741 tatcggatcg acaatccaca gccccttttc ccccgaatat tgttcccaca aagccgactg
     1801 ttcctgccgc gacatttcct cgcccaacat cgggcaatcg atcagaagcg gcaacgcggc
     1861 ggcaaatgcc gtctgcgcgg caatgtccgc ctcgctcaac atcgaaccat cttccttgcg
     1921 gcaagacggc gtattcaaaa aacgcggcat aatttcggtt tgcgcgatat ggcgcacgac
     1981 tttctgcaaa cggtgtaaca cttcctactg tcctcatatt ttgaacttgc ggcgcgcgaa
     2041 cgtatatagt ggattaaatt taaatcagga caaggcgacg aagccgcaga cagtacaaat
     2101 agtacggcaa ggcgaggcaa cgctgtactg gtttaaattt aatccactat aatgtccgtt
     2161 tccatcacgc cgctgcgaca gattataacc gtccgaaccg ccaaaaacta tgccccgatt
     2221 ccacctgccc gaaaaccttt ccgtcggaca aaccgtcgcc ttgcccgaca acatcgtccg
     2281 ccacctcaac gtcctgcgcg tccgccccaa cgaaaacatc accctcttcg acggcaaagg
     2341 caaggcacac gccgcacggc tgtctttttt ggaaaaacgc cgcgccgaag ccgaaatcct
     2401 gcacgaagac acaaccgaca acgaatcccc gctcaacatt acactgatac aatccatctc
     2461 ctccggcgat cgcatggatt tcaccctgca aaaaagcgtc gaactcggcg taaccgccat
     2521 acagcccgtc atcagcgaac gctgcatcgt ccgcctcgac ggggaacgcg ccgccaaacg
     2581 cctcgcacgc tggcaggaaa tcgtcatctc cgcgtgcgaa caaagcggca gaaacaccgt
     2641 tccccccgta ctgcccatca tcggctatcg tgaagcactc gacaaaatgc cgtctgaaag
     2701 caccaagctg attatgagca tcaaccgcgc ccgcaaactc ggcgacatcc gccacccatc
     2761 cggcgcaatc gtctttatgg tcgggcccga aggcggctgg acagaacagg aagaacaaca
     2821 ggcatttgaa gccggctttc aggcggttac actcggcaaa cggattttac gcacagaaac
     2881 cgccccactc gccgccctcg ccgcgatgca gacgctttgg ggcgatttcg cataaacaga
     2941 aatgccgtct gaaacccgtt cagacggcat tttgcagccg attaagatag taggttcaaa
     3001 taagatttcc cgtgtcgtca ttcccgcgaa agcgggaatc tagaaacgaa aaactacagg
     3061 gatttatccg aaacaacaac cctctccgcc gtcattcccg cgcaggcggg aatctagaaa
     3121 cgaaaaacta cagggattta tccgaaacaa caaaccctct ccgccgtcat tcccgcgcag
     3181 gcgggaatct agaaatttaa cgttgcggtg atttatcgga aatgactgaa actcaacgga
     3241 ctggattccc gcctgcgcgg gaatgacaaa gtggaagttg cccgaaact
//
LOCUS       DAWFLT010000133         3273 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWFLT010000133 DAWFLT010000000
VERSION     DAWFLT010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.133"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(465..941)
                     /gene="greA"
                     /locus_tag="WGR74_002069"
     CDS             complement(465..941)
                     /gene="greA"
                     /locus_tag="WGR74_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ0906399.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            1333..2634
                     /gene="aroA"
                     /locus_tag="WGR74_002070"
     CDS             1333..2634
                     /gene="aroA"
                     /locus_tag="WGR74_002070"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ0906400.1"
                     /translation="MTESLRLPAASLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYRLHGVARMHERPIGDLADALRIAGADVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FACELEKIGADVVWGENFVEVSRPKERAVQAFDLDANHIPDAAMTLAVVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVAEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLVSLLDVPVVINDPKCTHKTFPTYFDVFSSLTETAE"
     gene            complement(2677..3210)
                     /locus_tag="WGR74_002071"
     CDS             complement(2677..3210)
                     /locus_tag="WGR74_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0906401.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          695 a          878 c          880 g          820 t
ORIGIN      
        1 ttcaatgcct caagaattta tcggaaaaaa cagaaaccgt tctgccgtca ttcccgcgca
       61 ggcgggaatc tagaacgtgg aatctaagaa accgttttat ccgataagtt tccgtgcgga
      121 caagtttgga ttcccgcttt tgcgggaatg acgggatttt aggtttctga ttttggtttt
      181 ctgtttttga ggaatgacgg gatgcaggtt ttcttaaccc tgcgtcctag attcccgctt
      241 ttgcgggaat gacggcgaca gggttgctgt tatagtggat gaacaaaaac cggtacggcg
      301 ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctcaagcacc aagtgaatcg
      361 gttccgtact atctgtactg tctgcggctt cgtcgccttg tcctgatttt tattaatcca
      421 ctataatttc ctgcgtgtgt cgggtgtatc gaaatcaagc cgaatcaaat atatcggact
      481 tcgataatgt cgtattcgcg cacgccgccc ggggcttgga cttccgccgt atcgccctct
      541 tccttgccga ttaaggcgcg ggcaatcggt gagccgacat agattttgcc ctgtttgata
      601 tccgcttcgt cttcgccgac aatttggtag gtaacgtgtt cttccgtttc caaatcttcc
      661 agcgtaaccg tcgtaccgaa cacgattttg ccttcggcgt ggatttcggt cggattgatg
      721 atgtgggcaa cggaaagttt gtgttccagc tcggaaatgc ggccttcgat aaagccttgg
      781 cgttctttgg cggcttcata ttcggcgttt tcggacaaat cgccgtgcga tctggcttca
      841 gcaatcgctt cgatcacttc ggggcgcgcc acgcttttaa gctgctgcaa ttcctgtttc
      901 agcaattccg caccgcgtac ggtcaggggg attttttgca tcggtctttt ttctccatat
      961 tccggcacac cggtttgcgg cagcaaacat accgcatacc gtcttgtttt gtgcgtccgg
     1021 atattaaaat aaaaacacaa gccgcccgga aaatcggcgg cttgtctgtc gttgaacagc
     1081 ggctattcta ccaaattcta tgaaattggc aatcgtgccg tgccgtccgc aaacacgcta
     1141 tgtccgcaac aaaagccgaa aatatgccgg caaaaaaatt ttagaaacaa aaaatttaaa
     1201 aataatcaat tttcggcaca aaaaaccata ttcacggact ttaaaaccga aaatgccaaa
     1261 cctgagattt ttcagacggc atttgcacca gtataatgca ggctgttttt atctttatat
     1321 ttacgttttg ccatgaccga atccctccgc ctccctgccg cctcgctcaa accttccact
     1381 gtcgccctgc ccggctccaa aagcatcagc aaccgcaccc tgctgcttgc cgccttgtcc
     1441 gacaatgctt gcgaaatcca ttccctgctc aaatccgacg ataccgaccg tatgctcgaa
     1501 gcactcgata aactcggcgt tgaaatcgaa tatcttgccg aagaccgtct gaaagtgcac
     1561 ggcacaggcg gacgcttccc caaccgcact gccgatttgt ttttgggcaa cgcgggcacg
     1621 gcgttccgcc cgctgaccgc cgcgcttgcc gttttgggcg gcgattatcg cctgcacggc
     1681 gttgcgcgta tgcacgaacg ccccatcggc gatttggccg atgcactgcg gattgccggc
     1741 gccgatgtcg aatatctcgg caaggaacac tatccgccgc ttcatatcgg cgaacgccaa
     1801 gacaacggcg agcgcgtgat tccgattaaa ggcaatgtat ccagccagtt tctgaccgcc
     1861 cttttaatgg cgttgccgct gaccgggcag gcgtttgaaa tccgtatggt cggcgagtta
     1921 atttccaagc cctatatcga cattacttta aaactgatgg cgcaattcgg cgtacaggtt
     1981 atcaatgaag gctaccgcgt cttcaaaatt cccgccgatg cgcactacca cgcgcccgaa
     2041 cacttgcacg tcgaaggcga tgcctccagc gcgtcctact tcctcgcagc cggtttgatt
     2101 gccgccacgc ccgtccgcgt taccggtatc ggcgcaaaca gcatacaggg cgatgtcgcc
     2161 tttgcctgcg agctggaaaa aatcggtgcg gacgtggttt ggggcgaaaa cttcgtcgaa
     2221 gtctcgcgcc cgaaagaacg tgccgtccaa gcctttgatt tggatgcaaa ccatatcccc
     2281 gatgccgcca tgaccctcgc cgtcgtcgcg cttgctacag ggcaaacctg cacgcttcgc
     2341 aacatcggtt cgtggcgcgt caaagagacc gaccgcattg ccgcaatggc gaacgagttg
     2401 cgcaaactcg gtgcaaaagt cgccgaagaa gccgaagcaa ttcacatcac cccgcccgaa
     2461 acgccgacac ccgacgccgt catcgacact tacgacgacc accgcatggc aatgtgtttc
     2521 tcgctggttt cgctgttgga cgtacccgtc gtcatcaacg atccgaaatg cacccacaaa
     2581 accttcccga cttatttcga cgtgttctca tcgctgaccg aaacagcgga ataaggcggc
     2641 attttgccgc gattccggcg cggcgcggcg ggcggctcat tctgtaaaga aagtatgtgc
     2701 gccgaggtag tttttggcgt aaaacggtgt ggagagtttt tcggttttga tggttttgcc
     2761 gctgctgggg gcatggatga attcgccgtt gccgatatag agtccgacgt gtgagtagcg
     2821 gtgtgcgccg ccggtgttga agaatacgag gtcgccggcc ttaaggcggc tgtcggggat
     2881 tttgcggctt gccgccgcca tgtcgcgggc ggtgcgcggc agcttgacgt tgagggcgtt
     2941 tttgtaaacg aattgaatca tgccgctgca atcgaagccg gttgcggtgc tgctgccgcc
     3001 ccatttgtag ggcgtgccga tgagtccgag gctgtggagc atgagttcct gcgagccttg
     3061 tgtgcggtcg atgtggctga tgcggacggc ttggatttgc cggactgtct gtttgggttt
     3121 cggttggcgg tgtttgccgg aggtcgtgcc gcatgaggcg aggagcagtg cgctgagaca
     3181 gaggaaaagg gttttgtcgg ggggaaacat ggtttttcct ttgcgggttc ggatatgccg
     3241 tctgaaggtg tttcagacgg tatagtggat taa
//
LOCUS       DAWFLT010000134         3256 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWFLT010000134 DAWFLT010000000
VERSION     DAWFLT010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.134"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            584..907
                     /locus_tag="WGR74_002072"
     CDS             584..907
                     /locus_tag="WGR74_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ0906402.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            1021..3198
                     /locus_tag="WGR74_002073"
     CDS             1021..3198
                     /locus_tag="WGR74_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0906403.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLDADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTISQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDLSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          803 a         1113 c          819 g          521 t
ORIGIN      
        1 cgataacgct ttcacaaagc ctgtgtgcac tgaaaactga aaggtcatct gaaaacggag
       61 ctttcgaagg aagcagagtt tctgcgaagc taaaaccgaa gctgcaaaaa atcgaaaagc
      121 agcctgcacg ttgaaaatgc cgcccaagca aacctgcggt ttgccgccct ctccctagcc
      181 ctctcccaca gggagagggg attggggtgc aggctgtctt taaggttcag gcaaattttt
      241 aacttcgttg aagctgcgat ttcagaagct ccgttttagc ttcgcagaaa ctccgcttcc
      301 ttcgaaagct ccgttttcag acgacctttt ggaacaccac aggcacacgc atcgaacggc
      361 tgaatcaaag attcagaccg atggcagtcc gcacccgagt ttatgcggca aacagcgagg
      421 ctacggcaac ccgtcccctc tccctgtggg agagggttag ggagagggcg gtaagccgca
      481 ggcttacatc aaagccgata acgcttccgt tacaactccg cccactgaac gaagccaaaa
      541 cgacaaaccg catcgtaaac cacccaaccc ataggagaac cccatgcaaa acgaaaccat
      601 caacctgaaa cagcaccttg ccgccatcaa agaatactgg cagcccgaaa tcatcaaccg
      661 ccacgggttc caattccgtt tggtcaaact tttgggcgat tacggctggc atacgcacgg
      721 atacagcgac aaagtgctgt ttgccgtgga gggcgacatg gcggtggact tcgccgacgg
      781 cggcagcatg acgatacgcg agggcgagat ggcggtcgtg ccgaagtcgg tgtcgcaccg
      841 cccgcgttcg gaaaacggct gctcgttggt gctgattgag ttgtccgacc cgtccgaggc
      901 cgtctgaaaa cgaagtttcc gaaggaagct gagtttctgc gaagctaaaa gcagcctgca
      961 ccttcaatca atatgccgaa aatacaaccc caccgcacac caacacacaa aggaaatccc
     1021 atgacacgct tcaaatattc cctgctgttt gccgccctgt tgcccgtgta cgcgcaggcc
     1081 gatgtttctg tttcagacga ccccaaaccg caggaaagca ctgaattgcc gaccatcacc
     1141 gttaccgccg accgcaccgc gagttccaac gacggctaca ctgtttccgg cacgcacacc
     1201 ccgctcgggc tgcccatgac cctgcgcgaa atcccgcaga gcgtcagcgt catcacatcg
     1261 caacaaatgc gcgaccaaaa catcaaaacg ctcgaccgcg ccctgttgca ggcgaccggc
     1321 accagccgcc agatttacgg ctccgaccgc gcgggctaca actacctgtt cgcgcgcggc
     1381 agccgcatcg ccaactacca aatcaacggc atccccgttg ccgacgcgct ggccgatacg
     1441 ggcaatgcca acaccgccgc ctatgagcgc gtagaagtcg tgcgcggcgt ggcggggctg
     1501 ctggacggca cgggcgagcc ttccgccacc gtcaatctgg tgcgcaaacg cccgacccgc
     1561 aagccattgt ttgaagtccg cgccgaagcg ggcaaccgca aacatttcgg gctggacgcg
     1621 gacgtatcgg gcagcctgaa caccgaaggc acgctgcgcg gccgcctggt ttccaccttc
     1681 ggacgcggcg actcgtggcg gcagcgcgaa cgcagccgcg atgccgaact ctacggcatt
     1741 ttggaatacg acatcgcacc gcaaacccgc gtccacgcag gcatggacta ccagcaggcg
     1801 aaagaaaccg ccgacgcgcc gctcagctac gccgtgtacg acagccaagg ttatgccacc
     1861 gccttcggcc cgaaagacaa ccccgccaca aattgggcga acagccgcca ccgtgcgctc
     1921 aacctgtttg ccggcatcga acaccgcttc aaccaagact ggaaactcaa agccgaatac
     1981 gactacaccc gcagccgctt ccgccagccc tacggcgtag caggcgtgct ttccatcgac
     2041 cacaacaccg ccgccaccga cctgattccc ggttattggc acgccgaccc gcgcacccac
     2101 agcgccagcg tgtcattgat cggcaaatac cgcctgttcg gccgcgaaca cgatttaatc
     2161 gcgggtatca acggttacaa atacgccagc aacaaatacg gcgaacgcag catcatcccc
     2221 aacgccattc ccaacgccta cgaattttcc cgcacgggcg cctatccgca gcctgcatcg
     2281 tttgcccaaa ccatctcgca atacggcacc aggcggcaaa tcggcggcta tctcgccacc
     2341 cgtttccgcg ccgccgacaa cctttcgttg atactcggcg gcagatacag ccgttaccgc
     2401 accggcagct acgacagccg cacacaaggc atgacctatg tgtccgccaa ccgtttcacc
     2461 ccctacacag gcatcgtgtt cgacctgacc ggcaacctgt cgctttacgg ctcgtacagc
     2521 agcctgttcg tcccgcaatc gcaaaaagac gaacacggca gctacctgaa acccgtaacc
     2581 ggcaacaatc tggaagccgg catcaaaggc gaatggcttg aaggccgtct gaacgcatcc
     2641 gccgccgtgt accgcgcccg taaaaacaac ctcgccaccg cagcaggacg cgacctgagc
     2701 ggcaacacct actaccgcgc cgccaaccaa gccaaaaccc acggctggga aatcgaagtc
     2761 ggcggccgca tcactcccga atggcagata caggcaggtt acagccaaag caaaacccgc
     2821 gaccaagacg gcagccgcct gaaccccgac agcgtacccg aacgcagctt caaactcttc
     2881 actgcctacc actttgcccc cgaagccccc agcggctgga ccatcggcgc aggcgtgcgc
     2941 tggcagagcg aaacccacac cgaccctgcc acgctccgca tccccaaccc cgccgccaaa
     3001 gcccgcgccg ccgacaacag ccgccaaaaa gcctacgccg tcgccgacat catggcgcgt
     3061 taccgcttca atccgcgcgc cgaactgtcg ctgaacgtgg acaacctgtt caacaaacac
     3121 taccgcaccc agcctgaccg ccacagttac ggcgcactgc ggacagtgaa cgcggcgttt
     3181 acctatcggt ttaaataagg tcgtctgaaa acggagtttc tgcgaagcta tagtggatta
     3241 acaaaaacca gtacgg
//
LOCUS       DAWFLT010000135         3213 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWFLT010000135 DAWFLT010000000
VERSION     DAWFLT010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.135"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..631
                     /locus_tag="WGR74_002074"
     CDS             <1..631
                     /locus_tag="WGR74_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0906404.1"
                     /translation="QLTEKGLKIEKASAAVVDATIIQTAGSTQRQVIEVDEEGQINGQ
                     TTPSKDSDARWIKKNGLYKLGYKQHTRTDAEGYIEKLHITPANAHECKHLSPLLEGLP
                     KGTTVYADKGYDSKENRQHLKEHQLLDGIMRKACRNRPLSETQTKRNRYLSKTRYVVE
                     QSFGTLHRKFRYARAAYFGLLKVSAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            735..1292
                     /locus_tag="WGR74_002075"
     CDS             735..1292
                     /locus_tag="WGR74_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEZ0906405.1"
                     /translation="MGFGCREGKEFCKGLSLPEIIRDLLEMPSETAIADITRTYSAHY
                     LNPNNRNMTLFPDALPCLDKLKAQGYWLAVATGKGRAGLDNAISQTATGGYWLATACA
                     GEYPSKPSPEMVFGLCGELGLDPKEALVVGDTAHDLHMAANAGAAAVGVATGAHSREQ
                     LLSAPHLAVLDDLSELPGFLAQHYA"
     gene            complement(1368..2336)
                     /gene="ispH"
                     /locus_tag="WGR74_002076"
                     /gene_synonym="lytB"
     CDS             complement(1368..2336)
                     /gene="ispH"
                     /locus_tag="WGR74_002076"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ0906406.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(2365..2862)
                     /locus_tag="WGR74_002077"
     CDS             complement(2365..2862)
                     /locus_tag="WGR74_002077"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ0906407.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKY"
BASE COUNT          790 a          939 c          797 g          687 t
ORIGIN      
        1 acaactgacc gaaaaaggct taaaaataga gaaagcatcc gccgccgtcg ttgatgccac
       61 cattattcag accgccggca gcacacagcg tcaggtcata gaagtcgatg aagaaggaca
      121 aatcaacggc caaaccacac cgagtaagga cagcgatgcc cgttggatca agaaaaacgg
      181 cctctacaaa ctcggttaca aacaacatac ccgtaccgat gcggaaggct atatcgagaa
      241 actgcacatt acccccgcca atgcccatga gtgcaaacac ctgtcgccgt tgttggaagg
      301 actgcccaaa ggtacgaccg tctatgccga caaaggctac gacagtaagg aaaaccggca
      361 gcatctgaaa gaacatcagt tactggacgg cattatgcgc aaagcctgcc gcaaccgtcc
      421 gctgtcggaa acgcaaacca aacgcaaccg gtatttgtcg aagactcgtt atgtggttga
      481 acagagcttc ggtacgctgc accgtaaatt ccgctatgcg cgggcagcct atttcgggct
      541 gctcaaagtg agtgcgcaaa gccatctgaa agcgatgtgt ttgaacctgt tgaaagccgc
      601 caacaggcta agtgcgcccg ctgccgccta aaaggcagcc cggatgcctg attatcgggt
      661 atccggggag gattaagggg ggatttgggt agaattagga ggtatttggg gcgaaaatag
      721 acgaaaacct gtgtttgggt ttcggctgtc gggagggaaa ggaattttgc aaaggtctca
      781 gcctgcccga aatcatccgc gacctgctcg aaatgccgtc tgaaaccgcc attgccgaca
      841 tcacacgcac ttattccgca cattacctca atcccaacaa ccgcaatatg acgctctttc
      901 ccgatgccct gccctgtctg gacaagctca aagcacaagg atactggctt gccgtcgcca
      961 cgggcaaagg gcgggcgggc ttggacaacg ccatcagcca aaccgccacc ggcggctatt
     1021 ggctcgccac cgcctgcgcg ggggaatatc cctccaaacc ctcgcccgaa atggtattcg
     1081 gactctgcgg cgaactggga ctcgatccga aagaagcatt ggtcgtcggc gatacggcgc
     1141 acgacctgca tatggcggca aacgcaggcg cggcggcagt cggcgtggcc accggcgcac
     1201 attcgcgcga acagctcctt agcgcaccgc atctcgccgt attggacgac ttgtccgaac
     1261 tgcccggttt tcttgcacaa cattacgcct gattggtttc cgcatcagac actcgaaaca
     1321 aatgccgtct gaagcctgtt cagacggcat ttgtgttgcc caaacattca acgtttgcac
     1381 aaatcgggtt tggtttcgcc ctcgcggcgc agctctttgg gcaggacgaa cacaatgctt
     1441 tcttccgcac cctcgccttc gcgtacggtt tcgtgccccc atccgcgtat ggttgccagt
     1501 acttcccgca ccaacacttc gggcgcggac gcgcctgccg ttacgccgac tttgtttttg
     1561 ccctcaaacc atgcgcgttg caggtagcct gcattatcca ccatatacgc atcgattccg
     1621 cgcgatgccg ccacttcgcg caagcggttg ctgttggacg aattgggcga accgaccaca
     1681 atcacgatgt cgcactgttc tgccaactct ttgacggcgg tttgccggtt ggtcgtcgca
     1741 tagcagatat cttccttgtg cggattgcgg atattgggga aacgcgcgtt cagcgcggcg
     1801 atgatgtctt tggtttcatc gaccgagagc gtggtttggc tgacataggc gagtttgtcg
     1861 gggtttctga cttcgagttt tgccacatct ccgaccgttt cgaccaaaag cattttgccc
     1921 ggcgcaagct gccccatcgt gccttcgacc tcgacgtgcc ccttatgccc gatcatgatg
     1981 atttcacagt cttgggcatc cagtcgggcg acttccttat gcactttcgt caccagcggg
     2041 caagtcgcat caaacacgcg gaaaccgcgc tccgccgctt cttgctgcac cgccttcgat
     2101 acgccgtgtg ccgaataaac cagtgtcgcg cccggcggca cttccgccaa gtcttcaata
     2161 aacaccgcac ctttttcacg caggttgtcc acgacgaatt tgttgtgaac gacttcgtgg
     2221 cgcacataaa tcggcgcgcc gaactcttcc aaagcacgtt cgacaatact gattgcccga
     2281 tccacaccag cgcagaagcc gcgcggattg gcaaggatga tggttttctc gttcataagc
     2341 ccggtatttc gttttcagac ggcatcaata tttttcttct tgggttttac ggtggacgat
     2401 gttgtccaac accgccaaca ccgcaccgac gcagataaag ctgtcggcaa tattaaaggc
     2461 gggataaaac caattttgcc aataaaacaa taagaaatcg acgacatgac cgtgtatcag
     2521 gcgatcgatg acattgccta acgcaccgcc gataatcatc gccgcacccg ttttgccgag
     2581 ggttgcaaac tcatcgcgca agatggcgcg taccaaatac gcgctcaccg ccaccgccag
     2641 caccaaaaaa aagtattttt gccagccgcc ctgatcggca aggaagctga acgccgcacc
     2701 cgggttgtac accaaggtca aatcgaaaaa ggaaggaatg acattgacgc gttcccgata
     2761 ctgaaacgac gacagcaccg cccacttcga ccactggtcc agcacgatgg cggcaagtgc
     2821 caatacccaa tagcgcgttt tacttgaaac agatgaagac atatttttca acagccggta
     2881 aaagagtacc attttacccg aaaaccccct ttcctgtacc cgaaacggca aatgccgtaa
     2941 tcttaaaacc cgtcattccc gacaacaccg taatctcgaa acccgtcatt cccgcgcagg
     3001 cgggaatcca gacctgtccg cacagaaact tatcggataa aaacagttgc ccaaaccccg
     3061 cgttctatag tggattaaat tcaaaccagt acggcattgc ctcgccttag ctcaaagaga
     3121 acgattctct aaggtgctga agcaccgagt gaatcggttc cgtactattt gtactgtctg
     3181 cggcttcgtt gccttgtcct gatttaaatt taa
//
LOCUS       DAWFLT010000136         3084 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWFLT010000136 DAWFLT010000000
VERSION     DAWFLT010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.136"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            113..2878
                     /locus_tag="WGR74_002078"
     CDS             113..2878
                     /locus_tag="WGR74_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0906408.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQEGALKFNSDSGKWER
                     DLQRQQWKYNPYKNYNNQELQKYIEDHDKSWRENLAPQYDITPIDPSSLKQQSAGNLF
                     KLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQK
                     YPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKNR
                     FPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALKK
                     DIYRLNYSTNTVGYRFGGEYTGYYSSEGEFKRAFGENSPTYKKHCNPSCGLYEPVLKK
                     YGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPERA
                     NTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGNIPSWVSSTG
                     LAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNAS
                     KEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYID
                     GTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRYI
                     DPLDAGNDAATQRYYSSFDPKDKDEEVTCNDDNTLCNGKYGGTSKSVLTNFARGRTFL
                     ITMSYKF"
     gene            2908..>3084
                     /locus_tag="WGR74_002079"
     CDS             2908..>3084
                     /locus_tag="WGR74_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906409.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYE"
BASE COUNT          874 a          765 c          745 g          700 t
ORIGIN      
        1 ccaattgaaa tcttcagacg gtatatcgaa tttacacttt ttaatgttta tgccgcctaa
       61 aaaaatgcta atatatttct taattgtctg actgtttatt gttgaggaaa atatgagatc
      121 ttctttccgg ttgaagccga tttgttttta tcttatgggt gttatgctat atcatcatag
      181 ttatgccgaa gatgcagggc gcgcgggcag cgaggcgcag atacaggttt tggaagatgt
      241 gcacgtcaag gcgaagcgcg taccgaaaga caaaaaagtg tttaccgatg cgcgtgccgt
      301 atcgacccgt caggatatat tcaaatccag cgaaaacctc gacaacatcg tacgcagcat
      361 ccccggtgcg tttacacagc aagataaaag ctcgggcatt gtgtctttga atattcgcgg
      421 cgacagcggg ttcgggcggg tcaatacgat ggtggacggc atcacgcaga ccttttattc
      481 gacttctacc gatgcgggca gggcaggcgg ttcatctcaa ttcggtgcat ctgtcgacag
      541 caattttatt gccggactgg atgtcgtcaa aggcagcttc agtggctcgg caggcatcaa
      601 cagccttgcc ggttcggcga atctgcggac tttaggcgtg gatgacgtcg ttcagggcaa
      661 taatacctac ggcctgctgc taaaaggtct gaccggcacc aattcaacca aaggtaatgc
      721 gatggcggcg ataggtgcgc gcaaatggct ggaaagcgga gcatctgtcg gtgtgcttta
      781 cgggcacagc aggcgcagcg tggcgcaaaa ttaccgcgtg ggcggcggcg ggcagcacat
      841 cggaaatttt ggcgcggaat atttggaacg gcgcaagcag cgatattttg tacaagaggg
      901 tgctttgaaa ttcaattccg acagcggaaa atgggagcgg gatttacaaa ggcaacagtg
      961 gaaatacaat ccgtataaaa attacaacaa ccaagaacta caaaaataca tcgaagacca
     1021 tgataaaagc tggcgggaaa acctggcgcc gcaatacgac atcaccccca tcgatccgtc
     1081 cagcctgaag cagcagtcgg caggcaatct gtttaaattg gaatacgacg gcgtattcaa
     1141 taaatacacg gcgcaatttc gcgatttaaa caccaaaatc ggcagccgca aaatcatcaa
     1201 ccgcaattat caattcaatt acggtttgtc tttgaacccg tataccaacc tcaatctgac
     1261 cgcagcctac aattcgggca ggcagaaata tccgaaaggg tcgaagttta caggctgggg
     1321 acttttaaag gattttgaaa cctacaacaa cgcgaaaatc ctcgacctca acaacaccgc
     1381 caccttccgg ctgccccgcg aaaccgagtt gcaaaccacg ttgggcttca attatttcca
     1441 caacgaatac ggcaaaaacc gctttcctga agaattgggg ctgtttttcg acggtcctga
     1501 tcaggacaac gggctttatt cctatttggg gcggtttaag ggcgataaag ggctgctgcc
     1561 ccaaaaatca accattgtcc aaccggccgg cagccaatat ttcaacacgt tctacttcga
     1621 tgccgcgctc aaaaaagaca tttaccgctt aaactacagc accaataccg tcggctaccg
     1681 tttcggcggc gaatatacgg gctattacag ctcggaaggc gaatttaagc gggcattcgg
     1741 agaaaactcg ccgacataca agaaacattg caacccgagc tgcgggcttt atgaacccgt
     1801 attgaaaaaa tacggcaaaa agcgcgccaa caaccattcg gtcagcatta gtgcggactt
     1861 cggcgattat ttcatgccgt tcgccagcta ttcgcgcaca caccgtatgc ccaacatcca
     1921 agaaatgtat ttttcccaaa tcggcgactc cggcgttcac accgccttaa aaccagagcg
     1981 cgcaaacact tggcaatttg gcttcaatac ctataaaaaa ggattgttaa aacaagatga
     2041 tacattagga ttaaaactgg tcggctaccg cagccgcatc gacaactaca tccacaacgt
     2101 ttacgggaaa tggtgggatt tgaacgggaa tattccgagc tgggtcagca gcaccgggct
     2161 tgcctacacc atccaacacc gcaatttcaa agacaaagta cacaaacacg gttttgagtt
     2221 ggagctgaat tacgattatg ggcgtttttt caccaacctt tcttacgcct atcaaaaaag
     2281 cacgcaaccg accaacttca gcgatgcgag cgaatcgccc aacaatgcgt ccaaagaaga
     2341 ccaactcaaa caaggttatg ggttgagcag ggtttccgcc ctgccgcgag attacggacg
     2401 tttggaagtc ggtacgcgct ggttgggcaa caaactgact ttgggcggcg cgatgcgcta
     2461 tttcggcaag agcatccgcg cgacggcaga agaacgctat atcgacggca ccaacggggg
     2521 aaataccagc aatgtccggc aactgggcaa gcgttccatc aaacaaaccg aaacccttgc
     2581 ccgccagcct ttgatttttg atttttacgc cgcttacgag ccgaagaaaa accttatttt
     2641 ccgcgccgaa gtcaaaaatc tgttcgacag gcgttatatc gatccgctcg atgcgggcaa
     2701 tgatgcggca acgcagcgtt attacagttc gttcgacccg aaagacaagg acgaagaagt
     2761 aacgtgtaat gatgataaca cgttatgcaa cggcaaatac ggcggcacaa gcaaaagcgt
     2821 attgaccaat tttgcacgcg gacgcacctt tttgataacg atgagctaca agttttaaag
     2881 gcagcccgcg ttttgtggaa aaccgcaatg ccgtctgaaa gcccttcaga cggcatttgt
     2941 ttccccaaac gcatcatcct gccgcaagcc tatgccaatc cgttttatcg catcggcaac
     3001 tcaaagaaaa atccatttca ttcccacgca gggaagccgg tttttgattt cggttatttt
     3061 tggttgtttc gggtaattta tgag
//
LOCUS       DAWFLT010000137         3041 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWFLT010000137 DAWFLT010000000
VERSION     DAWFLT010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.137"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            45..2303
                     /locus_tag="WGR74_002080"
     CDS             45..2303
                     /locus_tag="WGR74_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ0906410.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINAFNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMRHGVLRRSFTVFGVRFNVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALGGSQTDWQVSNSFEVEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRNGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPGAWTGYAFHINYRGRLIKVAVGKENVVFSLLKGGPLDLQVYGKDI
                     TLNGSHTAALEK"
     gene            2316..2981
                     /gene="pgmB"
                     /locus_tag="WGR74_002081"
     CDS             2316..2981
                     /gene="pgmB"
                     /locus_tag="WGR74_002081"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ0906411.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYVEMIQAVKPEDVYPGILPLLET
                     LKANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRHCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSETAGLTYAYLQNVWAQ
                     SGR"
BASE COUNT          766 a          934 c          790 g          551 t
ORIGIN      
        1 gatttcacca ttccctcaaa tcaatccaaa caggagcttc ataaatgtac acaagaatca
       61 tggaaatcag cccttggacg ctgcgttcgg caaaactgga aaaagaacac aaacggctgc
      121 aagagagcct gaccagcttg ggcaacggct atatgggtat gcgcggcagc tttgaggaaa
      181 cctattccgc cgacagccac ttaggcacct acatcgccgg cgtgtggttc cccgacaaaa
      241 cccgcgtcgg ctggtggaaa aacggctatc ccaaatattt cggcaaagcc atcaacgcgt
      301 tcaatttcag caaagtcaaa atctttgtcg acgggcagga agtggacttg gcgaaaaacg
      361 acgttgccgg cttctccgtc gaactcgata tgcggcacgg cgtgttgcgc cgctcgttta
      421 ccgtattcgg tgtgcgtttc aatgtgtgca aattcctgtc cgtcgcgcaa aaagagctgg
      481 cggtcatccg ctgggaagcc gtatccgttg acggtaaaac ccaccaagtc cgcatcgatt
      541 ccatcatcga tgccgacgtg aaaaacgaag actccaacta cgaagaaaaa ttctggcagg
      601 tattggacaa aggcgtttca gacagtctct cctacattgc cgcccaaacc gttgccaacc
      661 ctttcggcgt ggaacaattt atcgtcaacg ccgagcaaac tttcgccggc agcttcaaag
      721 ccctcggcgg cagccaaacc gactggcaag tctccaattc tttcgaggtc gaagtcggcg
      781 gcacgcccaa aacctttgaa aaacgggtta ttgttaccac cagccgcgat tatcagggct
      841 tggaagcagt gaaagccgca ggccgcgcct tgtcggaaaa agtcgcaggc gttgcgtttg
      901 aaaccttgct ggacgcgcac aaagcaggct ggctgcaccg ttgggaaatc gccgacgtgg
      961 tcatcgaagg cagcgacgaa gcgcagcaag gcatccgttt caatctgttc caactgttct
     1021 ccacctacta cggcgaagat gcgcgcctga acatcggccc caaaggcttt accggcgaaa
     1081 aatacggcgg cgcgacctat tgggataccg aagcctatgc cgtaccgctc tacctcgcac
     1141 tggcggaacc cgaagttacc cgcaacctgc tgcaataccg ccgcaaccaa ctgccgcagg
     1201 cgcagcacaa cgcgcgcgaa cagggcttgg cgggcgcact ctatccgatg gtaacgttta
     1261 cgggtatcga gtgccacaac gaatgggaaa tcaccttcga ggaaatccac cgcaacggcg
     1321 cgattcctta cgccatctac aactacacca actacaccgg cgacgagggc tatcttgcca
     1381 aagaaggctt ggaagtcttg gtcgaagtgt cccgcttctg ggcggaccgc gtccacttct
     1441 ccaaacgcaa cggcaaatac atgattcacg gcgtaaccgg cccgaacgaa tacgaaaaca
     1501 acatcaacaa caactggtac accaacaccc tcgccgcctg ggtattggac tacacccgcg
     1561 aagccctggc gaaatacccg cgcccggatt tgaacgtgcg tgccgccgag ttggaaaaat
     1621 gggcggacat cagcgcgaat atgtaccgtc cgcatgacga agaactcggc gtattcgtcc
     1681 aacacgacgg cttcctcgac aaagacatcc gtcccgtgtc cgcgctttcg cccgacgatt
     1741 tgccgctcaa ccagaaatgg tcgtgggaca aaatcctgcg ttcgcccttc atcaagcagg
     1801 cggacgtgtt gcaaggcatc tacttcttcg gcgaccgttt caatatcgac gaaaaacgcc
     1861 gcaacttcga tttctacgaa ccgatgaccg tgcatgaaag ctcgctgtcg ccctgtatcc
     1921 actctattct cgccgccgaa ctgggcaaag aagaaaaagc cgtggaaatg taccggcgca
     1981 ccgcccgcct ggacttggac aactacaaca acgacacaga agacggcctg catatcacct
     2041 ccatgaccgg ttcgtggctc gccatcgtcc aaggtttcgc ccaaatgaaa acttggggcg
     2101 gcaaactcag cttcgcaccg ttcctgccgg gcgcgtggac aggctatgcc ttccacatca
     2161 actaccgagg ccgtctgatt aaagtcgccg tcggcaaaga aaacgttgtc ttcagcctgc
     2221 tcaaaggcgg gccgctcgat ttgcaggtgt acggcaaaga catcacgctc aacggcagcc
     2281 acaccgccgc gttggaaaaa taaggagggc gcaaaatgac gtttaccgca gtcctcttcg
     2341 atctcgacgg cgtgattacc gataccgccg aataccacta ccgcgcgtgg aaaaagctcg
     2401 ccgaagaact gggcatcggc atcgaccgca agtttaacga acagctcaaa ggcgtgtcgc
     2461 gcgacgattc gctcaaacgc atcctcgcgc acggcggcaa aaccgtcggc gaagccgagt
     2521 tcgccgaact gacccgccga aaaaacgaca actacgtcga gatgattcag gcagtcaaac
     2581 ccgaagacgt gtaccccggc attttgccgc tgctggagac attaaaagca aacggcaaaa
     2641 aaatcgccct cgcgtccgcc agtaaaaacg gcccgttcct gctcgaacgc atggggctga
     2701 cccacttttt cgacactgtc gccgaccctg ccgccgtcgc gcattccaaa cccgcccccg
     2761 acatcttcct cgccgccgcc gagggcgtgg gcgcggacat ccgccactgc atcggcattg
     2821 aagacgccgc cgccggcgtt gccgccatca aagccgccgg cgcactgccc atcggcgtag
     2881 gcaaagccga agacttgggc agcgacatcg cgctggtgtc cgagaccgcc gggctgacct
     2941 acgcctacct gcaaaacgtg tgggcgcagt cgggcaggta aagcgcgtca gataaagtgt
     3001 taaggaagta aaaggccgtc tgaacagtgt ttcagacggc c
//
LOCUS       DAWFLT010000138         2953 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWFLT010000138 DAWFLT010000000
VERSION     DAWFLT010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.138"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(139..1449)
                     /locus_tag="WGR74_002082"
     CDS             complement(139..1449)
                     /locus_tag="WGR74_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ0906412.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDGLKKLVAKVLALPEYRDFTIEADAQELFVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLEWLKSLDEEVLKAK"
     gene            1761..2183
                     /locus_tag="WGR74_002083"
     CDS             1761..2183
                     /locus_tag="WGR74_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906413.1"
                     /translation="MEWVSLFSDPQRDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITTGKNDNGNLTVNGIYGIQSFADSQLGYTPELLVKSNNDILFKNNFQSVDVKPHS
                     GNIHLDAKGKIQFNLNVPDVDGVQDVASGKKYRQTIVP"
     gene            2269..2766
                     /locus_tag="WGR74_002084"
     CDS             2269..2766
                     /locus_tag="WGR74_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HEZ0906414.1"
                     /translation="MNVKVHKDTHHFGIHNLNNTDVHLTAEDSDNVIEIKGELADLMP
                     TGNVESTSFLRGIETAFKSNTVLTGNNNKISIATEGKGLGLGVNGLQSNSPYSGHTTG
                     IFINNQSSDLTQPSLDGISTIKLKAVKGNNEVDLNVKNHASVKGIIASHSAKATLEAE
                     GDNIV"
     gene            2871..2953
                     /locus_tag="WGR74_002085"
                     /pseudo
     CDS             2871..2953
                     /locus_tag="WGR74_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
BASE COUNT          808 a          714 c          716 g          715 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataccac cttgtcttta ttggctttat cgataaacag
       61 gataagacct gaaaaaagcc gatacgcctt tttggtgtac cggctttgcc atactgttct
      121 gcttcagacg gcatcgcttc attttgcctt taatacttct tcgtccagcg atttcaacca
      181 ttccagcttt tcgccgattt tgatttccaa cccgcgcggg acgggttggt agaagtccgg
      241 ttcgtccaaa ccgtcgggca tatagctttc gccggcggcg taggcgttcg gttcgtcgtg
      301 ggcgtagcgg tattcgcgtc cgtagcccaa ttccttcatc agcttggtcg gggcgttgcg
      361 caggtggacg ggcacttcgt cgctggcgtt ttctttgacg aagtggcgca tttggttgta
      421 tgccttgtag cccgcgttgg atttggcggc ggcagcaagg tacagcacgg cttgagccaa
      481 agccagttcg ccttcgggcg agcctaagcg ttcgaaggtg gcggcggcat cgttggcgat
      541 ttggaaggcg cgcgggtcgg caagcccgat gtcttcccaa gcgatacgca cgatgcggcg
      601 ggcgaggtag cgcgggtcgg tgccgccgtc gagcatacgg cagaaccaat acagcgcggc
      661 gttcggatgc gaaccgcgca cggatttgtg cagggcggag atttggttgt agaagctctc
      721 gccgcctttg tcgaaacggc ggatttgcgc cccgagactg tcggcgagaa attcggcggt
      781 taagtttttc agacgacgtg tatcggcggc gcgtaaaagt tgttccaaca aattcaacaa
      841 tctgcgcgca tcaccgtcgg cggtattgac gaacagctcc tgcgcgtcgg cttcaatcgt
      901 gaaatcccgg tattcgggca aagccaaaac tttggcaacc agctttttga ggccgtctga
      961 agacaagggc tgcaaaacat acacctgcgc gcggctcaac agcgcgggat tgacttcaaa
     1021 cgacggattt tccgtcgtcg caccaataaa ggttagcaaa ccgctttcga catgcggcaa
     1081 aaacgcgtcc tgctgcgcct tgttgaagcg gtggacttca tcgacaaaca aaatcgtcgc
     1141 gcgtccctgc tgcaaagcga tttcggcttt atcgattgcc tcgcggatgt ccttcacgcc
     1201 ggaaaacacg gcggaaacag gcaaaaactg ggcattgaaa ctctgcgcca aaatccgcgc
     1261 caacgtcgtc ttgcccacgc ccggcggccc ccacagcaac atagaatgcg gcttgccgcc
     1321 ttctaccgcc acgcgcaaag gtttaccttc gccgatgagg tgttcctgcc ccaccacgtc
     1381 gtcaagcgta tgcggacgca atcgttcggc aagcggcgcg tcgggttctc gggcaaacaa
     1441 atcggtcata acggctccgt caacaggttt tcaaacaata tgattatacg gcagggaacg
     1501 gcggcgtgcc gcatacggat tccgcccctc cgtttgcctt aagccgatat taggcgcata
     1561 ctggaaaaga cgagagactt cacacaatat atccggcacg gagaccgatt ccgcatcggc
     1621 atgacaatac ccaaatcagc gtttcaatta aacattaagg agactaaaat agaaaatttg
     1681 ctttatctac cattgctttg ttgattcaat cggcattatg ttttgaggcg gaagcccatg
     1741 aatatactaa tattcaagag atggaatggg tgtctttatt ttctgatccg caaagagacg
     1801 atgatagtct tataaccctt aaagatgaaa aaatcactgt aaaaaactat attgtgcctt
     1861 ggtggaaaaa aggtgaaaac tttagaaaat tagaacttat taccactggt aaaaatgata
     1921 atggaaattt aacggtaaat ggaatttacg gcatccaatc ttttgcagac tctcaattgg
     1981 gatatacgcc tgaattattg gtaaaaagca ataatgatat tctatttaaa aataatttcc
     2041 aatctgttga cgtgaagccc cattccggaa atatccattt ggatgccaaa ggaaaaattc
     2101 aatttaattt gaatgttccc gatgttgatg gggtacaaga tgtagccagt ggaaaaaaat
     2161 accgacaaac aatagtgcct tagtgcggta tgtcaacact atcagccccc cgatgaaaat
     2221 gacaaaggca gcatatcctt tcattcaaaa acaggcaatt tgttcaatat gaacgttaaa
     2281 gttcataaag acacacacca tttcggtatt cacaacctca acaataccga tgttcatctg
     2341 actgccgaag acagcgataa tgtgattgag attaaagggg aactcgccga ccttatgccg
     2401 acaggaaatg ttgagagtac aagtttttta aggggcattg aaacggcttt taaatccaat
     2461 accgtattaa cggggaataa caataaaata tccatcgcca ctgagggcaa gggcttggga
     2521 ttgggcgtaa atggtctcca gtccaactcc ccctattccg gacacacgac gggcattttc
     2581 atcaataatc aatcaagcga ccttacccaa ccttcccttg atggcatcag caccatcaaa
     2641 ctcaaagccg tcaagggaaa taatgaagtt gatttaaatg taaaaaacca cgcctccgta
     2701 aaaggcatta tcgcgtcgca ttctgcaaaa gcaacgcttg aagccgaagg ggacaatatt
     2761 gtctaggtta aaaatccgga caccaatacg gctttaatcg atgctttgaa aaaacaatac
     2821 cccaatgctt cgaaagaact atacagggtg ggggttcaat ccacgactgg aacagtacgc
     2881 atttcgccaa acggcaaaat cgcattaacg gaattgaaaa cttttggaat tgtacaaaac
     2941 ggcatttgcg tca
//
LOCUS       DAWFLT010000139         2764 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWFLT010000139 DAWFLT010000000
VERSION     DAWFLT010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.139"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            872..1384
                     /locus_tag="WGR74_002086"
     CDS             872..1384
                     /locus_tag="WGR74_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ0906415.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAVAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRQT
                     VQTLLKRLSR"
     gene            1464..2543
                     /gene="aroB"
                     /locus_tag="WGR74_002087"
     CDS             1464..2543
                     /gene="aroB"
                     /locus_tag="WGR74_002087"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ0906416.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT          682 a          821 c          665 g          596 t
ORIGIN      
        1 aaaaaacaga aacccgaaac agcaacttaa aacctcgtca ttcccgcaaa agcgggaatc
       61 cggtccgttc agtttcggtc gtttccgata aattcctgct gcttttcatt tctagattcc
      121 cactttcgtg ggaatgacgg cggagaggtt tttgttgttt cggagaagtt tctgcaaccc
      181 tagaatctcg ttatttccac aaaaaacagc aacccaaaac ggcaacttaa aaccccgtca
      241 ttcccgcgca ggcgggaatc taggtctgtc ggttcaggac cttatcggat aaaacggttt
      301 ctccaaccct gcgttctaga ttcccacttt cgtgggaatg acgggatatg ggtttccgtg
      361 cggacgtgtt cggatttccg cctgcgcggg aatgacggcg acagatgccc aacggtcttt
      421 atagtggatt aacaaaaatc aggacaaggc gacgaagccg cagacagtac aaatagtacg
      481 gaaccgattc acttggtgtt tcagcacctt agagaatcgt tctctttgag ctaaggcgag
      541 gcaacgctgt actggttttt gttaatccac tatagtattg ataaacatat tatcttcaat
      601 atattcaatt ggataattgt ttacctaagc aaagataatt gccttttcct gacaaataag
      661 tgaaatcaac ggattgtcaa aacacagcct gaaataaaaa acctccctga tttcttttat
      721 ttttccttaa aatcagaaag gttcgggatg gtcgggttat ttttccaaac gtaccgctgc
      781 cctgccgatt tcgtataaaa ttccgccgta acccgacaag cccgaaccct gtcgccccga
      841 aaggcggggt gtcaaacatt aaggaattgt gatgaaaaac tttaacggca aactcatcct
      901 catcggactg atgggcgcgg gcaaaaccac gctgggccgg caaatggcgc agcggctgga
      961 ttaccgtttt tacgacagcg atcacgaaat cgccgtagcc gccggcgttc ccatccccac
     1021 catatttgaa atggaaggcg aacagggatt ccgttcgcgc gaaaccgcca tactcaaaaa
     1081 gctggttatc ctgccccata tcgtcctgtc caccggcggc ggcgcggtgt taaaagaaga
     1141 aaaccgcgcc cttatccgca aaagcggcac ggtcgtctat ctgcacgccc cgcccgaaac
     1201 cctgctcgaa cgcacgcgct gcgacaacag ccgtcctttg ctgcaagttg ccgatccttt
     1261 ggcgaaatta cgtgaactct acgccgcacg cgaccccgtt taccgccaaa ccgccgactt
     1321 taccgtagaa tcggcaaact gccggcaaac cgtgcaaacc ctgctcaaac gcttatcccg
     1381 ataaaccggc atatgcgccg cgcccagaaa accaagccgc gccccgcccg gcgggccggc
     1441 ggttcaaact ttaaggaaca acaatgaaaa cactgaccgt acacaccccg tcccacagct
     1501 accccatctt tatcggcaac ggactgctgc cgcaggcagg aagcctgctc aaaccgcatt
     1561 tgggcaaacg cgccgccatc atcgccaacg aaaccgtcgc cccgctctac ctcggcacgc
     1621 ttcagacggc attggatgcg gcaggcgtat cccatttcag catcatcctg cccgacggcg
     1681 aggcgcacaa aaactggcag acgctcaacc tcatctttga cgggctgatg caaaaccgcg
     1741 ccgaacgcaa aaccacatta atcgcactgg gcggcggcgt gatcggcgac atggtcggct
     1801 ttgccgctgc cacctaccag cgcggcgcac cgttcgttca aataccgacc acactgttga
     1861 gtcaagtcga ctcatcggtg ggcggcaaaa ccgccatcaa ccacccgctc ggcaaaaata
     1921 tgatcggcgc attctaccag ccgcaggcgg tgcttgccga cttggacacg ctgcacaccc
     1981 tgcccgtccg cgaattgtcc gccggtatgg cggaagtcat caaatacggc gcgctcggcg
     2041 acatcggttt tttcgaatgg ctcgaacaaa acatatccgg actgatgacg ctcgatcggg
     2101 aaaaactcgc ccaagccgtg taccgctgct gccaaatgaa ggcagacatc gtcgcccaag
     2161 acgaaaccga acagggcata cgcgcatggc tcaacctcgg acacaccttc ggacacgcca
     2221 ttgaaaccga gatgggttac ggcacttggc tgcatggaga agccatcgcc gccggctgcg
     2281 tgttggcggc gcgtttgtcc gaacaactgg gcaaaacctc cgccgcagat accgcgcggc
     2341 tcgccgccct actcgaagcc gccggactgc cgtccgcacc gcccgtgttt gcctttgaaa
     2401 aatggctcga acatatgagc cacgataaaa aagtcagcgg cggcatcatg cgctttatcg
     2461 gtctgaaccg gctgggcgaa gccaacatca ccgaaattac cgacacggac atcctccgcc
     2521 gcaccctgca accgtatctc tgatttcctc tgccgatgtg ctgccgcgcg ggtttgacgc
     2581 acgatgatga ttttccatca tctttctccg taaaagcggg aatccggtcc gttcggtttc
     2641 ggtcgtttcc gataaattcc tgctgctttt catttctaga ttcccacttt cgtgggaatg
     2701 acggtggaga ggtttttgtt gtttcggaga agtttctgca accctagaat ctcgttattt
     2761 ccac
//
LOCUS       DAWFLT010000140         2496 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWFLT010000140 DAWFLT010000000
VERSION     DAWFLT010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.140"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            633..2411
                     /locus_tag="WGR74_002088"
     CDS             633..2411
                     /locus_tag="WGR74_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEZ0906417.1"
                     /translation="MNKIYRIIWNSALNAWVAVSELTRNHTKRASATVKTAVLATLLF
                     ATVQANATDEDEEEELESVQRSVVGSIQASMEGSGELETISLSMTNDSKEFVDPYIVV
                     TLKAGDNLKIKQNTNENTNASSFTYSLKKDLTGLINVETEKLSFGANGKKVNIISDTK
                     GLNFAKETAGTNGDTTVHLNGIGSTLTDTLAGSSASHVDAGNQSTHYTRAASIKDVLN
                     AGWNIKGVKTGSTTGQSENVDFVRTYDTVEFLSADTKTTTVNVESKDNGKRTEVKIGA
                     KTSVIKEKDGKLVTGKGKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQTG
                     QADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVMYDVNVGDALNVNQLQNSGWNL
                     DSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEISRNGKNIDIATSMAPQFSSV
                     SLGAGADAPTLSVDDEGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQLKGVAQNLN
                     NRIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDGG
                     NWIIKGTASGNSRGHFGASASVGYQW"
BASE COUNT          775 a          614 c          613 g          494 t
ORIGIN      
        1 aatccataga aaaatcacag gaatctatca gaaaaaacag aaacccccac cgcgtcattc
       61 ccgcaaaagc gggaatccaa aaacgcaacg caacaggaat ctatcggaaa aaacagaaac
      121 cccaccgccg tcattcccgc aaaagcggga atccagaccc gtcggcacgg aatcttaccg
      181 gataaaacag tttccttaga ttccacgtcc tagattcccg ccttcgcggg aatgacgaga
      241 ttttagatta tgggaattta tcaggaatga ttgaatccat agaaaaacca caggaatcta
      301 tcagaaaaaa cagaaacccc caccgccgtc attcccgcgc aggcgggaat ccagcaacac
      361 aacgcggcag gactttatcg gaaaaaacag aaaccccacc gaccgtcatt cccgcaaaag
      421 cgggaatcca gacccgtcgg cacggaatct taccggataa aacagtttcc ttagattcca
      481 cgtcccagat tcccgccttc gcgggaatga cgagatttta agttggggga atttatcaga
      541 aaacccccaa cccccaaaaa ccgggcggat gccgcaccat ccgcccccaa accccgattt
      601 aaccattcaa acaaaccaaa agaaaaaaca aaatgaacaa aatataccgc atcatttgga
      661 atagtgccct caatgcctgg gtcgccgtat ccgagctcac acgcaaccac accaaacgcg
      721 cctccgcaac cgtgaagacc gccgtattgg cgacactgtt gtttgcaacg gttcaggcga
      781 atgctaccga tgaagatgaa gaagaagagt tagaatccgt acaacgctct gtcgtaggga
      841 gcattcaagc cagtatggaa ggcagcggcg aattggaaac gatatcatta tcaatgacta
      901 acgacagcaa ggaatttgta gacccataca tagtagttac cctcaaagcc ggcgacaacc
      961 tgaaaatcaa acaaaacacc aatgaaaaca ccaatgccag tagcttcacc tactcgctga
     1021 aaaaagacct cacaggcctg atcaatgttg aaactgaaaa attatcgttt ggcgcaaacg
     1081 gcaagaaagt caacatcata agcgacacca aaggcttgaa tttcgcgaaa gaaacggctg
     1141 ggacgaacgg cgacaccacg gttcatctga acggtatcgg ttcgactttg accgatacgc
     1201 ttgcgggttc ttctgcttct cacgttgatg cgggtaacca aagtacacat tacactcgtg
     1261 cagcaagtat taaggatgtg ttgaatgcgg gttggaatat taagggtgtt aaaactggct
     1321 caacaactgg tcaatcagaa aatgtcgatt tcgtccgcac ttacgacaca gtcgagttct
     1381 tgagcgcaga tacgaaaaca acgactgtta atgtggaaag caaagacaac ggcaagagaa
     1441 ccgaagttaa aatcggtgcg aagacttctg ttattaaaga aaaagacggt aagttggtta
     1501 ctggtaaagg caaaggcgag aatggttctt ctacagacga aggcgaaggc ttagtgactg
     1561 caaaagaagt gattgatgca gtaaacaagg ctggttggag aatgaaaaca acaaccgcta
     1621 atggtcaaac aggtcaagct gacaagtttg aaaccgttac atcaggcaca aatgtaacct
     1681 ttgctagtgg taaaggtaca actgcgactg taagtaaaga tgatcaaggc aacatcactg
     1741 ttatgtatga tgtaaatgtc ggcgatgccc taaacgtcaa tcagctgcaa aacagcggtt
     1801 ggaatttgga ttccaaagcg gttgcaggtt cttcgggcaa agtcatcagc ggcaatgttt
     1861 cgccgagcaa gggaaagatg gatgaaaccg tcaacattaa tgccggcaac aacatcgaga
     1921 ttagccgcaa cggtaaaaat atcgacatcg ccacttcgat ggcgccgcag ttttccagcg
     1981 tttcgctcgg cgcgggggca gatgcgccca ctttaagcgt ggatgacgag ggcgcgttga
     2041 atgtcggcag caaggatgcc aacaaacccg tccgcattac caatgtcgcc ccgggcgtta
     2101 aagaggggga tgttacaaac gtcgcacaac ttaaaggcgt ggcgcaaaac ttgaacaacc
     2161 gcatcgacaa tgtggacggc aacgcgcgtg cgggcatcgc ccaagcgatt gcaaccgcag
     2221 gtctggttca ggcgtatctg cccggcaaga gtatgatggc gatcggcggc ggcacttatc
     2281 gcggcgaagc cggttacgcc atcggctact ccagtatttc cgacggcgga aattggatta
     2341 tcaaaggcac ggcttccggc aattcgcgcg gccatttcgg tgcttccgca tctgtcggtt
     2401 atcagtggta agggcttttt cgcctgtctg ctgttgggac aggcggaagg tttgaaggga
     2461 agggtggcga tttgccgcct gagacctttg caaaat
//
LOCUS       DAWFLT010000141         2468 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWFLT010000141 DAWFLT010000000
VERSION     DAWFLT010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.141"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(13..819)
                     /locus_tag="WGR74_002089"
     CDS             complement(13..819)
                     /locus_tag="WGR74_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ0906418.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGLGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYSLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1040..2410
                     /gene="ffh"
                     /locus_tag="WGR74_002090"
     CDS             1040..2410
                     /gene="ffh"
                     /locus_tag="WGR74_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ0906419.1"
                     /translation="MLDNLTGRFSNVLKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVVGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTAGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
BASE COUNT          684 a          622 c          639 g          523 t
ORIGIN      
        1 tgttggctct tcttatcttt tcagaatgat ttccaatacg aacttgctgc ccatataggc
       61 aatcataagg ctgacaaatc cgatgatggt ccacacggcg gcttttttac cgcgccatgc
      121 ggtcatgctg tgcttgagca gcagtccgcc gtaaatcagc catgacaata tgccgaatac
      181 ggttttatgg gtaaaggtca tgggtttgcc gaatacggct tcggcaaaaa atgttccgct
      241 gacgacggaa taagtcagca ggatgaaacc tgcccacatg gcctggaaca tgagtttttc
      301 caaactgagc agcgacggca ggaatcctgc gagcttggag aagttcctgc ggtgcaggct
      361 ccgattcagc agcagggtca aaacggacaa taatgttgcg atgccgaaca gcccgtatgc
      421 gagcagcgaa gttccgatat gcagcataaa gggaaggtcg gtaatttcat atcccgagaa
      481 ttttccagga aaaaccaaac ctgacagcag catcagtgcg gcgcaaggat acagcagcaa
      541 ctgcactccg cgcagcgaat aaaagaagct gccggcaaaa taaataaaca gcatcatcca
      601 aacaatcagg ctgccggaat acccaaagcc cataatgatg attttgtctt gaatgaccgg
      661 cataagcagt gccgcgccat ggacggtcaa tgccgcgccc aaaaccggca attccgtctt
      721 ccacgggtaa tcccggccga gcccctgctg ttggcagtgc catgcaaatg cacccaatcc
      781 tgcgtaaacc gccgtcaaaa agatgaaaac tgtcggcatg gtggactttc tctaatctat
      841 actgttgcgc tgtatgcggc cgcttatgaa atattggaac ttttaacgtt ggaattgtaa
      901 aatccccgtt tcgggcaagc cttgacggat ttgccgatat gctgtccggc acacaagccg
      961 catcaaatta ttttgatttt attttaacaa agaatgcccc tgatggggca agctattctt
     1021 attcagacca aggaccagta tgttagacaa tttaaccggc cgcttcagca atgttctcaa
     1081 aaacatccgc gggcaggcca aactgaccga agacaatatt aaagaagcct tgcgcgaagt
     1141 ccgcctcgcc ctgcttgagg cggatgtcgc cctgcctgtc gtcaaagagt tcgtcaacaa
     1201 cgtcaaagaa aaggccctcg gtcaggaagt ggtgggcagc ctgacgccgg atcaggcatt
     1261 tatcggcgtg gtcaacaaag ccctgaccga gctgatgggc agggaaaaca aaacgctgga
     1321 tttgtcggtt gcgccgcccg ccgtcgtgtt gatggcaggt ttgcagggcg cgggcaagac
     1381 gacgaccgcc ggcaaactcg cccgcctgct gaaaaacgat cagaagaaaa aggttttggt
     1441 cgtatctgcc gacgtttacc gccctgccgc gattgaacaa ctgcgtctgt tggccgaaca
     1501 ggtcggcgtg gactttttcc catccgatac caaccaaaaa ccggttgaga ttgcaactgc
     1561 cgccgtcgat tacgccaaaa aacattttta cgatgtattg atggtcgata ccgccggccg
     1621 tttggcaatc gatgaggaga tgatgaacga aatcaaagcc cttcacgcgg cggttaaccc
     1681 ggtggaaact ttgttcgtca tcgatgcgat gctgggtcag gatgcggtga acactgctca
     1741 ggcatttaat gaagccctgc cgctgaccgg tgtcgtattg accaagatgg acggcgattc
     1801 gcgcggcggc gcagctttgt ccgtgcgcca cgtaaccggc aagccgatta aatttatcgg
     1861 tgtcggcgaa aaaatcaacg gcctcgaacc tttccacccc gaccgtcttg ccagccgtat
     1921 cctcggcatg ggcgacgtat tgaccctgat tgaagacgtc caaaaaggta ttgatgaaga
     1981 agccgccgcc aaaatggcga aaaagctaca aaaaggcaaa ggcttcgacc tcaacgactt
     2041 taaagaacaa atccagcaaa tgcgcaatat gggcggtttg gaaaacctga tgtcgaaaat
     2101 gccgggcgaa ctgggtcaaa tatcgaaaca aatccccgaa ggaacggctg aaaaagcgat
     2161 gggcaaagtg gaagccatca tcaactcgat gactccaaaa gaacgcgcca accctgccct
     2221 gctcaaagcc agccgcaaac gccgtattgc aatgggtgcg ggcacgaccg tacaggaagt
     2281 gaacaaattg ctcaaacagt ttgaacaaat gcaacaaatg atgaagatgt tcagcggcaa
     2341 cggcttgggc aaactgatgc gtatggcgaa aggaatgaag ggaatgaaag gaatgttccc
     2401 gggtttgtaa gccgatttga ccgaaaacgc cgtctaaaat ttcagacggc gtttttgttt
     2461 tatattct
//
LOCUS       DAWFLT010000142         2458 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWFLT010000142 DAWFLT010000000
VERSION     DAWFLT010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.142"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            35..1390
                     /locus_tag="WGR74_002091"
     CDS             35..1390
                     /locus_tag="WGR74_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ0906420.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWFELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2404
                     /locus_tag="WGR74_002092"
     CDS             1394..2404
                     /locus_tag="WGR74_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ0906421.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     SYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDLEVF
                     DFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGDTREATIAYGIES
                     LS"
BASE COUNT          453 a          660 c          740 g          605 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcaaatg agccgcattt ttcaaacgct
      181 aggcgcagac ccgcacaatt tgggctggtt tttcatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt tgggcggccg
      301 ccgtctgccg tatctgcttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttggacg tgtcgtcaaa tatggcgatg cagccgttta agatgatggt
      481 cggcgacatg gtcaacgagg agcagaaagg ctacgcctac gggattcaaa gtttcttagc
      541 gaatacgggc gcggtcgtgg cggcgattct gccgtttgtg tttgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccgca gaccgtggtc gtggcgtttt atgtgggtgc
      661 ggcgttgctg gtgattacca gcgcgttcac gattttcaaa gtgaaggaat acgatccgga
      721 aacctacgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggtt
      781 cgaactctta aaaaccgcgc ctaaagcgtt ttggacggtt actctggtac agtttttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccacc gatgcgtctt ccgtaggtta tcaggaggcg ggcaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtaacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggcc tgtttaacgg ctctatctgt atgcctcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tccctatgct gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttctgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccgcgat tttggcctga tcgacgggcg
     1441 tgccgtaacc ggctatgtgc tgtccaaccg gcgtggtacg cgtgtctgcg tgctggactt
     1501 gggcgggatt gtgcaggaat tttccgtttt ggcagacggc gtgcgcgaaa acctcgtggt
     1561 gtcgttcgac gatgcggctt cctatgcgga caatccgttt cagattaaca agcagatagg
     1621 gcgcgtggcc ggacgcatcc gcggtgcggc gttcgacatc aacggcagga cttaccgcgt
     1681 ggaggccaac gaaggcagga acgcgctgca cggcggttcg cacgggctgg ccgttacccg
     1741 tttcaacgcg gtggcggcag acggccgttc ggtggtgctg cgcagccgcc tgcaacagtc
     1801 ggccgacggt tatcccaacg atttggattt ggatatttcc taccgcttgg acgaggacga
     1861 ccggcttacc gttagctatc gcgccaccgc gctcggcgac acggtgttcg acccgacgct
     1921 gcacatttac tggcggctgg acgcgggcct gcacgatgcg gttctgcata ttccgcaggg
     1981 cggacatatt ccggccgatg ccgaaaaact gcccgtctca acggtttcag acgacctcga
     2041 agtatttgat ttcagccggc ccaagccgct ggatgccgcc gttgccgccc tgcgccgcga
     2101 aacgggtcgg gccggttttg acgacgctta ccgcgtgccg tccgatatag gccgtcccgc
     2161 cgctgtgttg caagccggac gccgccgtcg tatcagcata tacagcgacc gcaatggctt
     2221 ggtcatcttt accgccgccc cgcaggattt cgcgcggcac gatgcgggcg tttacgacgc
     2281 gctggcgacc gaggcgcaga cgctgcccga cagcctgaat tggcccgagt tcggcaatat
     2341 tcgtctgaac aagggtgata ccagggaggc gacgattgct tacggcatcg aatccctttc
     2401 ttaggagctt cctaacaccg gttgtagacg gcctttttat agtggattaa caaaaacc
//
LOCUS       DAWFLT010000143         2445 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWFLT010000143 DAWFLT010000000
VERSION     DAWFLT010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.143"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..597
                     /locus_tag="WGR74_002093"
     CDS             <1..597
                     /locus_tag="WGR74_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0906422.1"
                     /translation="YGYLARYTQDNATKWLSDMPGQDAYSINLIEISVYYKKTDQGWV
                     LEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERLLTYGVKKMPSAYPEY
                     EAYEDKRHIPENPYFHEFYYIKKGENPAIITHWNNRVNQAEEDNYSTSVGSCINGFTV
                     QYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
     gene            614..>2445
                     /gene="frpC"
                     /locus_tag="WGR74_002094"
     CDS             614..>2445
                     /gene="frpC"
                     /locus_tag="WGR74_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein FrpC"
                     /protein_id="HEZ0906423.1"
                     /translation="MNEGEVVLTPEQIQTLRGYASRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFRQYARLINQNNGR
                     LPNTSEIERSYYKAVTDNGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQA
                     VNNPNGSERDNRKQLISALDKGFDGSFKEKHFTFLQSVMMDVTKLGVEYTIDGWQKIG
                     GWGNGIINDLYKSVVKREWTGIFEIVNNNIKQGNEAFKNEINSLVHDMKAAGKEFGDD
                     LNTQWNNLTQAAEIIYNDIVDNTSQGIEKGVKAIKELSEKMKNAASDLADGSAEKAKQ
                     VVEDLAQAAKEAYENAKSTAEKAAQAAREFFKGLPSFKDLAEKFRDLFPNPEGWIDDG
                     HQCLAPWVKETKKRNGKYHVYDPLALDLDGDGIETVATKGFAGSLFDHTNNGIRTATG
                     WVSADDGLLVRDLNGNGIIDNGAELFGDNTKLADGSFAKHGYAALAELDSNGDNIINA
                     ADAAFQSLRVWQDLNQDGISQTNELRTLEELGIQSLDLAYKDVNKNLGNGNTLAQQGS
                     YTKTNGTTAKMGDLLLAADNLHSRFKDKVELTAEQAKAANLAGIG"
BASE COUNT          799 a          465 c          561 g          620 t
ORIGIN      
        1 tatggctatc ttgcacggta tacgcaagac aatgccacaa aatggctttc cgacatgccc
       61 gggcaggatg cttactccat taatttgata gagattagcg tctattacaa aaaaaccgac
      121 caaggctggg ttcttgagcc atacaaccag caaaacaaag cacactttat ccaatttcta
      181 cgcgacggtt tggatagcgt ggacgatatt gttatccgaa aagatgcgtg tagtttaagc
      241 acgactatgg gagaaagatt gcttacttac ggggttaaaa aaatgccatc tgcctatcct
      301 gaatacgagg cttatgaaga taaaagacat attcctgaaa atccatattt tcatgaattt
      361 tactatatta aaaaaggaga aaatccggcg attattactc attggaataa tcgagtaaac
      421 caggctgaag aagataatta tagcactagc gtaggttcct gtattaacgg tttcacggta
      481 cagtattacc cgtttattcg ggaaaagcag cagctcacac agcaggagtt ggtaggttat
      541 caccaacaag tagagcaatt ggtacagagt tttgtaaaca attcaagtaa aaaataattt
      601 aaaggatctt attatgaatg agggtgaagt tgttttaaca ccagaacaaa tccaaacctt
      661 gcgtggttat gcttcccgtg gcgataccta tggcggttgg cgttatttgg ctaatttggg
      721 tgaccgttat gcggatgatg ctgctgcaat tgtcggtaag gatgcaaact taaatggttt
      781 gaatttatgg atgaaaaaag gtgtggaaaa cctatgggat gatacggtcg gtaaaaagac
      841 ccgtttagag aaatttgatc gggttgcact gcaacatttc aggcaatatg cgcgtctaat
      901 taatcaaaat aatggtagat tacccaatac tagtgaaatt gagagaagtt actataaagc
      961 cgttaccgat aatggcgttt cttccagtgc agctattgat ttagttatta atcgttcact
     1021 tccggatatg gcggatggtt attgggcatt aggtttgggg atagaagccg aacgtatcca
     1081 caatgagcaa gcagtaaata atccgaacgg tagcgaaagg gataatagaa agcagttaat
     1141 atctgcttta gataaaggat ttgatggatc ttttaaagag aagcatttta cttttttaca
     1201 atctgtgatg atggatgtaa caaagttagg tgttgaatat acaatagatg gttggcaaaa
     1261 aattggaggt tggggtaatg ggataatcaa tgatttatat aaaagtgttg taaaaagaga
     1321 gtggactgga atatttgaga tcgttaataa taacatcaag caaggaaatg aagcttttaa
     1381 aaatgaaatc aatagcttgg ttcatgatat gaaagctgct ggcaaggaat ttggagatga
     1441 cttaaataca cagtggaata atctcactca ggctgccgaa ataatctata atgacatagt
     1501 agacaatact agtcaaggaa tagaaaaagg tgtcaaagcc attaaagaat tgtctgaaaa
     1561 aatgaaaaat gctgcttccg atttggctga cggttcagca gagaaagcta aacaagtagt
     1621 ggaagatttg gctcaagccg ccaaagaagc atacgaaaat gccaaatcca cagccgagaa
     1681 ggctgctcaa gcagctcgag aattttttaa gggcttgccc agttttaaag atctggccga
     1741 aaaatttaga gatctgttcc caaatccgga aggctggatc gatgatggtc accaatgttt
     1801 agctccttgg gttaaagaaa ctaaaaaacg caatggcaaa tatcatgtct acgaccccct
     1861 tgccctagac ctagatggcg acggtataga aaccgttgcc accaaaggct ttgcaggcag
     1921 cttatttgat cacaccaaca acggtatccg caccgccacc ggttgggttt ctgccgatga
     1981 cggtttactc gtccgcgatt tgaacggcaa cggcatcatc gacaacggtg cggaactctt
     2041 cggcgacaac accaaactgg cagacggctc ttttgccaaa cacggctacg cggctttggc
     2101 cgaattggat tcaaacggcg acaacatcat caacgcggca gacgccgcat tccaatccct
     2161 gcgtgtatgg caggatctca accaggacgg catttcccaa accaacgaac tccgcaccct
     2221 tgaagaattg ggtattcaat ctttggatct cgcctataaa gatgtaaata aaaatctcgg
     2281 taacggtaac actttggctc agcaaggcag ctacaccaaa acaaacggta caaccgcaaa
     2341 aatgggggat ttacttttag cagccgacaa cctgcacagc cgcttcaaag acaaagtgga
     2401 actcactgcc gaacaggcaa aagccgccaa tcttgcgggc attgg
//
LOCUS       DAWFLT010000144         2330 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWFLT010000144 DAWFLT010000000
VERSION     DAWFLT010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.144"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(59..1468)
                     /gene="dacB"
                     /locus_tag="WGR74_002095"
     CDS             complement(59..1468)
                     /gene="dacB"
                     /locus_tag="WGR74_002095"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ0906424.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGISDTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1633..2199
                     /locus_tag="WGR74_002096"
     CDS             1633..2199
                     /locus_tag="WGR74_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0906425.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTVFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          535 a          566 c          668 g          561 t
ORIGIN      
        1 agtttgtcct agttatctag gacagcccct taaatttaat ccactatatt ttcctgtttc
       61 aggctcggcg ttctttgcac atcagtttcg catccagcca gccatcgccg ccggagatga
      121 tgttgttggc aacgaagttg tccaagtcgg gcagcaggga aacggcgcgg ccgctgttga
      181 tgatgacgac caccgccatc ggtttgtcgc ccagccaata acctgcaagg gcgcggacat
      241 tgttgagcgt gccggttttt aagcgcaaca gcccgccgct ttgtttgaag cggttgcgta
      301 aagtcccgtc tgtgccggcg atgggcagcg tatcgatgaa atcttgtgca aacgggctga
      361 aataagccgt ttccaacatt tgcgccatca ttctcgccgt taccctttct tttctggaca
      421 gacctgaacc gttttccaaa accaaatccg caacatcgat gcccgacacg gcaagttcac
      481 gccggacggc agacgctgcc tgttcggaaa cggcgggcag tttgccgtcg ccgccgagtt
      541 tgaggaagac ggaacgcgca attagattgt ccgaacgctt gttcatgtcc gtcaaaattt
      601 ccttcatcgg ctttgagtgt gcaacggcaa gcgtctgcgc gccttccggc gtgtcggata
      661 tgccgatgcc gtctgaaatc cgtccgccgc cgagcagcca gtggttggta aaactttgcc
      721 ggatcagttc gtcaagcgcg aacatccgga caccgacagg cttgcccaaa cagctctcgg
      781 gaatattgcc gcgcaatttc agcgtattgt ccgaaaaaga tgcacgcatc agttttttga
      841 tcgaagggca ggcagcttgg gaggcggtaa ttttcaagtt gttttgggcg aaaatatgcg
      901 gcaaaggcgg atcggtgagg atgtcggtac tgtcggcggc attgcgttcg gcgcgcacca
      961 taaccatacc ggcagacagc atagttggat tggggggcgt cataaacggc gaaccgctgt
     1021 cggcttcgaa atcgtcgggg ctgccgactt cgccccacag gctgtggtcg agcatcaggt
     1081 gtcccgtgat attgcgtatg ccttgttcgc gcaactggcg ttggacggca agcaggtttt
     1141 cctgattgaa aacggggtcg ccgctgcccg cccaatacag gtttccgtca agcgtgccgt
     1201 cgtttaccgt accgttgctt ttaaactcgg tcgcccagcg gtaattgctg ccgaaggttt
     1261 tgaaggcggc aaacgcggta acgagtttca ttgtggaggc ggggttgacg gggacatccg
     1321 agcggtggtc aatgatgact tttccgctgt caagctcttg gacatatacg gcgatttcgt
     1381 tttgcggaat gcgacctgta tcgagcgcgt gtgcggcgag ggaggcgaga agcagcagca
     1441 gggaggccgc tgttttgggg aaattcatag gtaaatctgt tacataaaaa aagcaattat
     1501 aaggcaaagc cggataagtg ggaacgctgt ggcggcgggc cggcttgttt gcaggggaaa
     1561 tcacatatat aataatcgct atcattatga aatgattgag acgcacaaac ttaatattca
     1621 ggaggaatga ttatggctaa gaaaatcagt attttggtgg gcagcctgcg ccgtgcttct
     1681 tttgcgcgca aagtggcatt gaatgcggcg gagatgttcc ccgaaggctg gcaggcggaa
     1741 atcgtcgaaa tcggacatct gccgctttac aatttcgatt atgacgaccc tgcggtggaa
     1801 gatgtgccgc tgcccgaaag ctacacggtt ttccgcgaaa cgattaaggc ttcggacggc
     1861 attttgttcg ttacgtccga aaataaccgc accattcccg cctgtttgaa aaatgcggtg
     1921 gacatcggct cgaaaccgaa tgccgacgtg gcgtggaaaa acaaaccggc cggcatcatc
     1981 agccattccg tcggcaagat gggcggttac agttcgcaaa aaaacctgcg ccttgcgctg
     2041 tcttatttcg atatgcccgt aaccggacag ccggaagtgt ttttgggcaa ttcgccgaca
     2101 ctgtttgatg aaaacggcaa attgatcgac tcggcaaggg attttgttca gtcatatatt
     2161 aatcagtttg tcggtttgat tgaaagaaat gccaaataaa caaacaagtc gaaaggccgc
     2221 aaaaccggtt cgggttttgc ggctttttta tatgggctgg gcagcgtcaa ggtttgccgt
     2281 ttgccggata atgcgcggca aatgaccggc gtgtttatag cggattaaca
//
LOCUS       DAWFLT010000145         2325 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWFLT010000145 DAWFLT010000000
VERSION     DAWFLT010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.145"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(44..631)
                     /locus_tag="WGR74_002097"
     CDS             complement(44..631)
                     /locus_tag="WGR74_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ0906426.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPSGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRFAALS"
     gene            794..2200
                     /gene="dnaB"
                     /locus_tag="WGR74_002098"
     CDS             794..2200
                     /gene="dnaB"
                     /locus_tag="WGR74_002098"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ0906427.1"
                     /translation="MNDYTAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          576 a          597 c          623 g          529 t
ORIGIN      
        1 accgcaaaaa tctacccgaa atgatatagc ggattaacaa aaatcaggac aaggcggcga
       61 agcgtttggc gacttcgtcc cagttgacga tttcccaaaa acctttcagg tagttggggc
      121 ggctgttgcg gtagtcgatg taataggcgt gttcccacac gtcgcaggtc agcagcggcg
      181 tgttttcagt ggtcagcggc gtagcggcgt tggaagtgga aaccaaatcc aatccgccgg
      241 caggggtttt caccagccat gcccaaccgg agccgaaagt accggcagcg caggcattga
      301 acgcttcttg gaatttctcg aagctgcccc atttcgcgtc gatggcggcg gccagttcgc
      361 cggaaggttt gccttgacct ttgggcgtga aacccagcca gtagaaggtg tggttccaag
      421 tttgcgccgc gttgttgaac acgccgcccg aagatttttt cacaatctct tccaaaggca
      481 ggttttcaaa ttcggtgcct ttgatttgat tattcaggtt ggtgatgtag gtttgatggt
      541 gtttgccgta gtggaactcc aaagtctctt tgctcagatg cggggacaat gcgtccagtt
      601 cataaggcag ttgcggcagc ttatgttcca ttttgtgctc ctaatattgt tttaaatgtt
      661 gtattttggc agtgttgctg caaatagctc agcagcgcac atattctacc tgttttgagg
      721 gatggaaacc aattaaacct gctttacgct ataatagaag attgcaattt cggcacgaca
      781 gataggatgt accatgaacg attacacagc tatgccgtct gaagaccgtg aggtcggcgc
      841 attgtcgctg cctccacatt cgatggaggc ggaacaatcc gttttgggcg gtttgatgct
      901 ggaaaatccg gcttgggaca ggattgccga tgtggtttcg ggagaggatt tctaccgcca
      961 cgaacaccgc ctgattttcc gatccattgc aaaattaatc aatgagagcc gtcccgccga
     1021 tgtgattacg gttcaggaag atttgcagcg gaacgaagaa ttggaagcgg caggcggatt
     1081 tgaatatctg attacgctgg cgcaaaacac cccgtctgcc gccaacatcc gccgctatgc
     1141 cgaaatcgtg cgcgagcgtt ccatcatgcg ccagctcgcc gaagtgggga cggaaatcgc
     1201 acgcagcgca tacaatccgc aaggcaggga cgcggggcag cttttggacg aggcggaaaa
     1261 caaagtattc caaatcgccg aaagcacggc caaatccaag cagggctttt tggagatgcc
     1321 cgatttgctg aaagaagtcg tacagcgcat cgatatgctc tactcgcgcg acaatcccga
     1381 tgaagttacc ggcgtgccga cagggttcat cgacctcgac aaaaaaacct cgggtctgca
     1441 acccggcgac ctgattatcg ttgccggtcg tccgtctatg ggtaagaccg ccttttccat
     1501 caatatcgcc gaacacgttg ccgtagaagg caagctgccg gttgccgtct tctcaatgga
     1561 gatgggcggt gcgcaactgg tcatgcgtat gctcggctcg gtcggacggt tggatcaaag
     1621 cgtcctgaaa acaggcaggc tcgaagacga acactggggt cgcctgaacg aagcagtcgt
     1681 caaactctcc gacgcgcccg tgtacatcga cgagaccccg ggtttgactg cgctcgaact
     1741 gcgcgcccgc gcccgccgtc tcgcccgtca atttaacaat aagctgggat taatcgtcat
     1801 cgactacctg caactgatgg cgggatccgg ccgttccgac aaccgagctt cagagctggg
     1861 cgaaatttca cgttcgctca aagcgttggc gaaagaattg caagtcccca tcatcgccct
     1921 gtcgcaactg agccgcacgg tcgaatcgcg taccgacaaa cgcccgatga tgtccgacct
     1981 tcgcgagtcc ggcgcaatcg agcaggatgc cgacctgatt atgtttatgt accgcgacga
     2041 atactacaac caagactcgc ccatgaaagg ccttgccgaa tgtatcatcg gcaaacaccg
     2101 caacggtccc gtcggtaaaa tcttcctcac atggacgggg caattcacca aattcgacaa
     2161 tgctgcctat attcccgagg aggcaaagat agaagattaa atggctatat aaaaatttat
     2221 taggcgaaat caggcaaaat cgtttaaaat catgctgaga gattgcccta aaaaataaaa
     2281 cgcggtcttg aggcattttt gcattcagcc cgcatataat tgaaa
//
LOCUS       DAWFLT010000146         2297 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWFLT010000146 DAWFLT010000000
VERSION     DAWFLT010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.146"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            1..719
                     /locus_tag="WGR74_002099"
                     /pseudo
     CDS             1..719
                     /locus_tag="WGR74_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            867..1046
                     /locus_tag="WGR74_002100"
                     /pseudo
     CDS             867..1046
                     /locus_tag="WGR74_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347026.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1043..1549)
                     /locus_tag="WGR74_002101"
     CDS             complement(1043..1549)
                     /locus_tag="WGR74_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ0906428.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     VEVEKKFG"
     gene            complement(1552..1749)
                     /locus_tag="WGR74_002102"
     CDS             complement(1552..1749)
                     /locus_tag="WGR74_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906429.1"
                     /translation="MSDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            complement(1746..2012)
                     /locus_tag="WGR74_002103"
     CDS             complement(1746..2012)
                     /locus_tag="WGR74_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906430.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADRDNIAASLGRIENKLEKVNEKLDRKADKS"
     gene            complement(2024..>2297)
                     /locus_tag="WGR74_002104"
     CDS             complement(2024..>2297)
                     /locus_tag="WGR74_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906431.1"
                     /translation="SRAREQEPFVIHVSDLLDKAGIDYAVRMDKDFQTTFHLEYPITN
                     YDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYAVRIVFGDVPV"
BASE COUNT          595 a          625 c          541 g          536 t
ORIGIN      
        1 tttgacgaac tgagcatccc cgattacagc accttatgcc gctaccgcaa ccggctggcg
       61 caagacaata ccctgtctga actgttggaa ctgattaacc gccaactgac cgaaaaaggt
      121 ttaaaaatag agaaagcatt gacgccacca ttattcagac tgccggcagc acacagcgtc
      181 aggctataga agtcgatgaa gaaggacaag tcggcggcca aaccacaccg agtaaggata
      241 aagatgcccg ttggacaaag aaaaacggcc tctacaaact cggttacaaa caacataccc
      301 gtaccgatgc agaaggctat atcgagaaac tgcacatcac ccccgccaat gcccatgagt
      361 gcaaccacct gttgcctttg ctggaaggta tagccgaagg cacgaccgtc tatgccgaca
      421 aaggctatga cagtgcggaa aaccggcaac atctggaaga gcgtcagttg ttagacggca
      481 ttatgcgcaa agcccaccgc aaccgtccgc tgacggaagt gcaaaccaaa cgcaaccggt
      541 atttatcgaa gacccgttat gtggtcgaac aaagcttcgg tacgctgcac cgtaaattcc
      601 gctatgcgcg ggcagcctat ttcgggctgc tcaaagtgag tgcgcaaagc cacctgaagg
      661 cgatgtgttt gaacctgttg aaagccgcca acaggttaag tgcgcccgct gccgcctaaa
      721 aggcagcccg gatgcctgat tatcgggtat ccggggagga ttaaggggat atttgggtaa
      781 aattaggagg tatttggggc gaaaacagcc gaaaacctgt gtttgggttt cggctgtcgg
      841 gagggaaagg aattttgcaa aggtctcggg ctttaattgc ggcaaaaata gaccaagcgg
      901 agatttctga ttcagaaaaa agctggctga aaaaagaatt agggaaaatc aaggacactg
      961 cattaagtac gcttacggaa aatgcaatta atgctatcct tgccgccacc ctcattactc
     1021 ttttaaaaaa atttgtcggc ctctaaccaa acttcttctc cacttccacc ccaaacggct
     1081 ccaatacctc cacaaaggca ggcaaatgtt tggggtgttt ttcttgcaca aacgccatca
     1141 agaactcaat caattccaaa gccgtcgcca gttttgacgt ttccggcatc acacgcgcat
     1201 tggcggatac ggttttcgta aacgcatcgg ccaggctggc caacagcttg gcgcggtcgg
     1261 acgggggcaa atcttcggta ctcgaatcct gcagcatcgt catcgtgctg ttgtactgca
     1321 ccataaaacc ggccaacatc gcacggctca agtcctcaat accgccgccg gccaaagtgt
     1381 aggcggcgcg catcttatcc cagtcgtcgc ctttttcctt atccgcacgt ttccacgcac
     1441 gcgcagtggt ctgcgggatt tcgcacatca aggccgccgt ttccaaagtt tgctcgccgc
     1501 tcacatagag ccgacgtaac ttttcacgga tttcttgcgg gtgagccata attacagtcc
     1561 cattttcgct ttaagcaatt cccagccgac cgtaatcacg ccgccgccca gtgcgccgaa
     1621 tgtaatggcc gtgcgtttcg tgtcttggcg gatttgcgca atttccgcct gcatttcttt
     1681 ctgatttttc agcgtttgat cagtcttgtt ttcaatacgc gccaaggctt ccaaaatcgg
     1741 gtcgctcatg atttgtctgc tttcctgtcc agtttttcgt ttactttttc caacttgttt
     1801 tcgatgcgtc ccaacgaagc cgcaatattg tcgcggtctg ccttggcatc ttgcttggtg
     1861 tgataagaga gcttgaccgc gtgcagctcc tctttcaggt cgtcgatacg cttgtccgcc
     1921 tctttcagac ggcctgaaat gccgttgacc caaaaccaaa atgccgccgt cgcaatcggc
     1981 cacagggttc tgaaaccaaa ttcaaagtcc atttaaaacc cctttaaacc ggcacatcgc
     2041 cgaatacgat acggacggcg taaccgtcag ggcgattgct ggcaatctcc agtccatcac
     2101 catcattaca aacacagtaa tacgccccca aagtttgcca aaccgcacgt ttaaaggtgt
     2161 catagttcgt aattggatat tcaaggtgaa acgtcgtctg aaaatcctta tccatgcgta
     2221 ccgcgtaatc gataccggcc ttatccaaca aatcggaaac atgaatgaca aacggctctt
     2281 gctcgcgtgc gcggctt
//
LOCUS       DAWFLT010000147         2166 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWFLT010000147 DAWFLT010000000
VERSION     DAWFLT010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.147"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            30..463
                     /locus_tag="WGR74_002105"
                     /pseudo
     CDS             30..463
                     /locus_tag="WGR74_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            559..1182
                     /gene="rsmG"
                     /locus_tag="WGR74_002106"
     CDS             559..1182
                     /gene="rsmG"
                     /locus_tag="WGR74_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ0906432.1"
                     /translation="MERKERLRAGIAALGLDISAAAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMVVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTAHLLKDG
                     GYWAAMKGVYPQGEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            1281..2054
                     /locus_tag="WGR74_002107"
     CDS             1281..2054
                     /locus_tag="WGR74_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ0906433.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKASLQSGVYQVLLGDADVKSAAVRSKEGGYGVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELMARVSGK"
BASE COUNT          431 a          493 c          741 g          501 t
ORIGIN      
        1 gcggtctgct tcggaagagg gggacggcga tgtttgtgaa cgagaaagat ccttatgcgg
       61 ctctgtttgc gggactggtg tttttgacgc tgccgtttgc gttggcggtg catgatgcct
      121 ttgcgcttgc gttcggacgg gcggggctgc tggtgtcggt gtcggacggc ggattcggct
      181 ggcgtggcgg ttgggacggc actgtttggt ttgtgttcgg tgtgtttgcg tttttgaatg
      241 tggttgtgtc ggcgggcctg acgaaactgg cgtacaaaaa gatgatgcgg cggcattcgc
      301 gtggattttc gagctgctgt ttggcagtgc cgccttgggc gggctgcgtg gggaggcggt
      361 gttggaatat gcgtttgccg tgtggctggt gtcgatgctg acactgccca aacgcttgac
      421 gcgcgcgccg gtgcagccgg tggtgtttca caggaaaaaa taggacggaa cgggggatgc
      481 cgtctgaaac ccgacacgcg gtttcagacg gcatgttttt ccgttaacat tacgcctgaa
      541 catggacagg atagggatat ggaacgcaaa gaacgcctgc gtgcaggcat cgccgcattg
      601 ggtttggata tttccgcagc ggcgcaggac aggcttttgg cctatgtgga tttgttgaaa
      661 aagtggaaca aaacctacaa tctgaccgcc ctgcgcgacg aggaaaaaat ggttgtccat
      721 catcttttgg acagcctgac gctgctgccc catatcgagg gtgtgcaaac gatgctggat
      781 gtcggttcgg gcggcggtca gcccggcatt ccggcggcgg tgtgccgtcc ggatgtgcaa
      841 ataacccttt tggatgcgaa tacgaagaaa acggcttttt tacagcaggc ggttatcgag
      901 ttggggttgg acaatgtgcg cgtggtatcc ggacgcgtgg aggcggtttc ggacgtgcgt
      961 gccgacgtgg ttaccagccg tgcgtttgca gaactggcgg actttgtatc gtggacggcg
     1021 catctgttga aagacggcgg ctactgggcg gcgatgaagg gcgtatatcc gcagggggaa
     1081 atcggccgcc tgccgcagga tgtgtgcgtt gaaaaagtcc aaaggctcga cgtgccaggc
     1141 ttggatgcgg aacgccatat cgtcatcctg agcaagcgtt gagcgcgctt cagacggcat
     1201 gaataccttt tttgtgcgga taaaggtaaa attccgcact gtttttcttt tttcaacatc
     1261 agacgggaca cgggcgggac atgagtgcga acatccttgc catcgccaat cagaagggcg
     1321 gtgtgggcaa aacgacgacg acggtaaatt tggcggcttc gctggcatcg cgcggcaaac
     1381 gcgtgctggt ggtcgatttg gatccgcagg gcaatgcgac gacgggcagc ggcatcgaca
     1441 aggcgagttt gcagtccggc gtttatcagg tcttattggg cgatgcggac gtgaaatcgg
     1501 cggcggtacg cagcaaagag ggcggatacg gcgtgttggg tgcgaaccgc gcgctggccg
     1561 gcgcggaaat cgagctggtg caggaaatcg cccgggaagt gcgtttgaaa aacgcgctca
     1621 aggcagtggc ggaagattac gactttatcc tgatcgactg cccgccttcg ctgacgctgt
     1681 tgacgcttaa cggcttggtg gcggcaggcg gcgtgattgt gccgatgttg tgcgaatatt
     1741 acgcgctgga agggatttcc gatttgattg cgaccgtgcg caaaatccgt caggcggtca
     1801 atcccgattt ggatatcacg ggcatcgtgc gtacgatgta cgacagccgc agcaggctag
     1861 ttgccgaagt cagcgaacag ttgcgcagcc atttcgggga tttgctgttt gaaaccgtca
     1921 tcccgcgcaa tatccgcctt gcggaagcgc cgagccacgg tatgccggtg atggcttatg
     1981 atgcgcaggc aaagggtgcc aaggcgtatc ttgccttggc ggacgagctg atggcgaggg
     2041 tgtcggggaa ataggtcaat ccaaatcggg ctgcccgtgc ctttatgctg tttggccggg
     2101 tgcgttaata tggcggatta aaataaaaat acttatatcg tcatttatag tggattaaca
     2161 aaaacc
//
LOCUS       DAWFLT010000148         2114 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWFLT010000148 DAWFLT010000000
VERSION     DAWFLT010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.148"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            413..1870
                     /locus_tag="WGR74_002108"
     CDS             413..1870
                     /locus_tag="WGR74_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0906434.1"
                     /translation="MSRHPARTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIVYFTRLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          485 a          619 c          487 g          523 t
ORIGIN      
        1 gacggtcttt atagcggatt aacaaaaatc aggacaaggc ggcgaagccg cagacagtac
       61 aaacagtacg gaaccaattc acttggtgct tcagcacctt agagaatcgt tctctttgag
      121 ctaaggcgag gcaacgccgt actggttttt gttaatccac tataaaaaac cgccctatcg
      181 gataagtcgc ttgactgaaa aggctttacc cgcgccgtat cggaacacgt cctctaaaat
      241 acaatccgtt gaattgaaac aaatataaaa acattcgacc gcaaaaacgg cagcgcatcg
      301 tttgacaaag attgaaaata tcggttaaaa accgattttc atacaaaaaa caccgctgcc
      361 gtccgcatcc gtttcagacg gtattgagag aaaatctttt aggagaacct ttatgtcccg
      421 gcatcccgcc cgcaccggag aaaaaacatt cttcggccac cccttccagc tttccaccct
      481 cttccatatc gaattgtggg aacgtttttc attttacgga atgcagggca tcctgctgat
      541 ttacctctac tacaccgccg acaaaggcgg cttgggcata gacaaaaccc tcgccggcgg
      601 cattgtcggc gcatacagcg gcagcgtgta cctgtccacc attttgggcg cgtggtttgc
      661 cgaccgcgta tggggtgcgg aaaagaccct cttcctctcg ggcatcgtcg tgatgctcgg
      721 acacatcgtc cttgccgccg ccccgggcct gtacggcctt ttaatcgggc tgatattcat
      781 cgcattgggc agcggcggtg tgaaatctac ggccagttct atggtgggcg cattatacga
      841 acaggacgaa atgcgcccgc tgcgcgatgc gggattttcc attttctaca tcgccatcaa
      901 tatcggcggc ttcctaggcc cgctgctgac cggcctgctg caggaaaaca tcggtttcca
      961 ttatggtttc ggcgcggcgg cggtcggtat ggcattcggc ttgtggcgtt attccttggg
     1021 acgtaaaaac ctgccccacc ccaccgtccc ccatccgctt tcaaaaggac agggcaaaac
     1081 tgcggccgcc gtcggcatcg ccctcatcgc cgcacttgca accgccatca aaaccgggct
     1141 tgtcaacctc gacaatttct ccggcatcct cctatctacc gtcatccttg ccgtcatcgt
     1201 ctattttacc cggctgctga ccaacccccg cgtcagttcc gacaacaagc ggcacatcat
     1261 cgcctacatc ccgcttttcc tgaccatctg tatgttttgg gcggtctggt ttcagattta
     1321 caccgtggca accgtctatt tcgacgaaac cgtcaaccgc accatcggtt cgtttaccgt
     1381 acccgtcgct tggaaagatt ctatgcaaag cctgtgggtc atcctgtttt ccggacttat
     1441 ggcggcaatg tggacaaaaa tggggcgcaa acagcccaaa accccgctga aattcgctat
     1501 ggcggtattt gttaccggcg cgtcgttttt gggattcgtc ccctttattt ccgccggcac
     1561 gccgatgcct attgcggttt tcgcactgat tgtccttgcc atcacgatag gcgaactgat
     1621 gatttccccg attgcgctgt ccatctccac caaaatcgca ccgcctttat tcaaaaccca
     1681 aatggtcgcc cttaatttcc ttgccttttc attaggcttc actttgggcg gcgtattgtt
     1741 tgaaaaaggc tatcaggcgg gcgacgaaat cggcttctat cggctgctgt tctacatcgg
     1801 cgcagccaca ggcttcctgc tgctcctgct cgtccccaaa ttgaacaaaa tgctcgaagg
     1861 cacagactaa gtcccgcccc gatgccgtct aaacccttca gacggcattt ttccgcataa
     1921 tgaaaccaaa ccgtttccac ccgacaggac aggctccccc ccaaccggaa ggcagcctgc
     1981 cgattgtcat ttaaatatct gactgattcc gtctgtttgc aagggaaata gccgtttatt
     2041 tccgcttggg cggaaaccgg ttctattgag taaaaggcat tttatccgac taaattagtg
     2101 ctgcatcaac ggaa
//
LOCUS       DAWFLT010000149         2099 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWFLT010000149 DAWFLT010000000
VERSION     DAWFLT010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.149"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..1443
                     /locus_tag="WGR74_002109"
     CDS             <1..1443
                     /locus_tag="WGR74_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0906435.1"
                     /translation="INGGSLKDNLGDAALGAIVSTVHGEVASKIKFNLSEDYITHKIA
                     HAIAGCAAAAANKGKCQDGAIGAAVGEIVGEALTNGKNPATLTAKEREQILAYSKLVA
                     GTVSGVVGGDVNTAANAAKVAIENNLLSQEEYALREKLIKKAKGKGLLSLDWGSLTEQ
                     EARQFIYLIEKDRYSNQLLDRYQKNPSSLNNQEKNILAYFINQTSGGNTAWAASILKT
                     PQSMGNLTIPSKDINNTLSKAYQTLSRYDSFDYKSAVAAQPALYLLNGPLGFSVKAAT
                     VAAGGYNIGQGAKAISNGEYLHGTVQVVNGTLMVAGSVSAQAAISAKPAPVTRYLSND
                     SAPALRQALTAESQRIRMKLPEEYRQIGNLAIAKIDVKGLPQRMEAFSSFQKGEHGFI
                     SLPETKIFKPISVDKYHNIASPPRGTLRNIDGEYKLLETIAQQLGNNRNVSGRIDLFT
                     ELKACQSCSNVILEFRNRYPNIQLNIFTGK"
     gene            1448..1831
                     /locus_tag="WGR74_002110"
     CDS             1448..1831
                     /locus_tag="WGR74_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906436.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
BASE COUNT          719 a          391 c          429 g          560 t
ORIGIN      
        1 atcaacggcg gcagcctcaa agacaacttg ggcgatgccg cactgggtgc gatagtcagt
       61 accgtacacg gagaagtagc gagcaaaatc aaatttaatc tcagcgaaga ctacattacc
      121 cacaagattg cccatgccat agcgggctgt gcggcagcgg cggcgaataa gggtaagtgt
      181 caggatggtg cgatcggtgc ggctgtgggc gagatagtcg gggaggcttt gacaaacggc
      241 aaaaatcctg ccactttgac agctaaagaa cgcgaacaga ttttggcata cagcaaactg
      301 gttgccggta cggtaagcgg tgtggtcggc ggcgatgtga atacagcggc gaatgcggct
      361 aaagtcgcga ttgaaaataa cctattatct caagaagagt atgctcttag agaaaaattg
      421 atcaaaaaag ccaaagggaa aggcctatta tctttagatt ggggcagcct gaccgaacaa
      481 gaggcaaggc agtttatcta tttgattgag aaagatcgat attctaatca attgcttgac
      541 cgatatcaaa aaaatccaag tagtttaaat aatcaagaaa aaaatattct tgcatatttt
      601 attaaccaaa cctctggagg taacacagct tgggcagctt cgatactgaa aacgccccag
      661 tcaatgggta atctcactat tccttccaaa gatattaata acaccttatc gaaagcctat
      721 caaacattga gtcgttatga ttcttttgat tacaaatcag ctgttgccgc acaacctgca
      781 ctttacttat taaacggacc gcttggcttc agtgtcaaag cagctactgt ggcagcagga
      841 ggatataaca ttggacaggg agcgaaagca atctctaatg gagaatatct gcatggtaca
      901 gttcaggttg ttaatggcac attgatggtt gcaggatctg tatctgcaca ggctgcaata
      961 tcggccaagc ctgcacctgt tacccgttat ctgagcaatg acagtgctcc tgctttaaga
     1021 caagctttaa ctgctgaaag ccagagaatc cgcatgaaac tgccggaaga gtatcgacaa
     1081 atagggaatc ttgcgatagc aaaaattgat gttaaaggat taccgcaaag gatggaagca
     1141 tttagttctt tccaaaaagg ggaacatgga tttatttcgt tacctgaaac aaaaattttt
     1201 aaacctatat ctgttgataa atatcataat attgcctctc ctcctagagg aacattaaga
     1261 aatatagatg gagaatataa attacttgaa actatagcac agcaactcgg aaataatcgt
     1321 aatgtatcag gtagaattga tctatttaca gaattaaagg cctgtcaatc ttgcagcaat
     1381 gttattttag agtttagaaa tcgctatcca aatattcaat taaatatttt tacaggaaaa
     1441 tagaatcatg gaaaaaataa aaaattatat agaattcaag aaattattta aaaatataac
     1501 ggaaaaattt gtcagagatt tagatataac attaattgaa gcagctgaat attctataat
     1561 tgaattcgaa atttatttat ctgaatatcc ggaagatagt acatttattt ttacagcatg
     1621 ggcaatctat ctgttagaaa ataactgtct caatgaaaga ggaaaagata attttttcat
     1681 cgcggaaatg aaaaaatgtt atcaacaaac taatttggat agaataaaat ctatctattc
     1741 ttcgagtgac tatcatattt ttgctaagga tgtaaacaag attcaacaaa catttaaatt
     1801 aaatcttgcc aatatcatgc agcaagagta gcaactgtat ttttaacccc gtcgggcaaa
     1861 attaccaaaa acggtaaatc actaaaaata cttttatacc gccgccgcag caagctgtag
     1921 cctgcatgaa acctaaaatc catgcgtaag gtgtgtgctc cagcacgcac gcgttccatg
     1981 atttacggct caatgccgtc tgaaaagctc acaatttttc agacggcatt tgttatgcaa
     2041 gtaaatattc agattcccta tatactgccc agatgcgtgc gtgctgaaga cacccccta
//
LOCUS       DAWFLT010000150         2051 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWFLT010000150 DAWFLT010000000
VERSION     DAWFLT010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.150"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(264..695)
                     /locus_tag="WGR74_002111"
     CDS             complement(264..695)
                     /locus_tag="WGR74_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906437.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEIQKTYSQTNCSLHET"
     gene            complement(711..>2051)
                     /locus_tag="WGR74_002112"
     CDS             complement(711..>2051)
                     /locus_tag="WGR74_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0906438.1"
                     /translation="VNGGSLKDNLEANILAALVNTAHGEAASKIKQLDQHYIVHKIAH
                     AIAGCAAAAANKGKCQDGAIGAAVGEIVGEALTNGKNPDTLTAKEREQILAYSKLVAG
                     TVSGVVGGDVNAAANAAEVAVKNNQLSDKEGREFDNEMTACAKQNNPQLCRKNTVKKY
                     QNVADKRLAASIAICTDISRSTECRTIRKQHLIDSRSLHSSWEAGLIGKDDEWYKLFS
                     KSYTQADLALQSYHLNTAAKSWLQSGNTKPLSEWMSDQGYTLISGVNPRFIPIPRGFV
                     KQNTPITNVKYPEGISFDTNLKRHLANADGFSQKQGIKGAHNRTNFMAELNSRGGRVK
                     SETQTDIEGITRIKYEIPTLDRTGKPDGGFKEISSIKTVYNPKKFSDDKILQMAQNAA
                     SQGYSKASKIAQNERTKSISERKNVIQFSETFDGIKFRSYFDVNTGRITNIHPE"
BASE COUNT          544 a          409 c          377 g          721 t
ORIGIN      
        1 gaactgatat ccgtggtgct gagcaggatg cgactgttct cattggcata cggtgcgcta
       61 atgctcacac cggttttacc cgaaagttct gcagcaagcg tagggggtgt cttcagcacg
      121 cacgcgtctg ggcagtatat agggaatctg aatatttact tgcataacaa atgccgtctg
      181 aaaaattgtg agcttttcag acggcattga gccgtaaatc atggaacgcg tgcgtgctga
      241 agcacacacc ttacgcatgg attttaggtt tcatgcaggc tacagtttgt ttgagaatat
      301 gttttttgta tttctttaca ctttttttga tattcagggt gagctattaa gaaatcagat
      361 attgcaatat taaagtaatg atatgctaaa ttaataggta taattaattc ttcaggattg
      421 aaatcatctc caactgaaaa ataaatagaa ggagctttat caatatactc aaatccattt
      481 acctcttcat aatctccaat ccatctggta gcatctattt tttcatattg agcacctatt
      541 actccaaaat gtccggtagt tgtaatattt ttaaaacact ctaaaagcgt atctttagtt
      601 tcaaattggt aaattgtttc aaaaaaaatc gaaataacaa aatggttgtt atttatagat
      661 tttttattta agtttagaaa aatattattt ttcataattt ttcctttaaa ttattctggg
      721 tgaatgtttg taattcttcc tgtatttaca tcaaaatatg atctaaattt gattccgtca
      781 aaggtttctg agaattgaat gacatttttt ctttccgata ttgatttagt tctttcattt
      841 tgagcaattt tagaggcttt tgaatatcct tgtgaagcag cattttgagc catttgaagt
      901 attttatcat cagaaaattt tttaggatta taaacagttt ttatacttga aatttcctta
      961 aatccaccat caggtttacc tgtcctgtct agtgtaggaa tctcatattt aattcgggta
     1021 atgccttcaa tatcagtttg ggtttcagat tttacgcgtc ctcctcgtga atttagttct
     1081 gccataaaat tggtgcggtt atgggctcct ttaatgccct gtttttgact aaaaccatca
     1141 gcatttgcca gatgtctttt taggtttgta tcgaaactga tgccttccgg gtatttgaca
     1201 ttagtaatag gtgtattttg ttttacaaac cctcttggta ttggaatgaa tctaggatta
     1261 actcctgaaa taagtgtata accttggtcg gacatccatt cggataaagg ctttgtattg
     1321 cccgattgaa gccaagattt agcagcagta ttcaaatgat aagactgtaa agccaaatct
     1381 gcttgggtgt aagatttgct gaataattta taccattcat catctttacc aattagacct
     1441 gcttcccaag atgaatgaag gcttctacta tcgatcaaat gttgttttct gattgttcta
     1501 cattcagtac tacgggatat atccgtacat attgcaatcg aagcagcaag tcttttatca
     1561 gcaacatttt gatacttttt tacagtattt tttctgcaca gttgaggatt attctgtttg
     1621 gcgcatgcag tcatttcgtt atcaaattct ctaccctctt tgtcgctaag ctgattattt
     1681 ttcaccgcta cctcagccgc attcgccgcc gcatttacat cgccgccgac cacaccgctt
     1741 accgtaccgg caaccagttt gctgtatgcc aaaatctgtt cgcgttcttt agctgtcaaa
     1801 gtgtcaggat ttttgccgtt tgtcaaagcc tccccgacta tctcgcccac agccgcacct
     1861 atcgcaccat cctgacactt gcccttattc gccgccgctg ccgcacagcc cgctatggca
     1921 tgggcaatct tgtggactat gtagtgctga tccaactgtt tgattttact ggctgcttct
     1981 ccatgcgcgg tattgaccaa agccgcaagg atattcgctt ccagattgtc tttcaggctg
     2041 ccgccgttga c
//
LOCUS       DAWFLT010000151         1943 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWFLT010000151 DAWFLT010000000
VERSION     DAWFLT010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.151"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            293..1480
                     /locus_tag="WGR74_002113"
     CDS             293..1480
                     /locus_tag="WGR74_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ0906439.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNYQLQLTEAQ
                     AANGGAGASGQVKVTKVTKAKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVS
                     VAGGGATQWGNRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFK
                     RHDDMSVSVRYDSPEFSGFSGSVQFVPAQNSKSAYTPAHFVQNKQNQRPTLVPAVVGK
                     PGSDVYYAGLNYKNGGFAGNYAFKYAKHANVGRDAFELFLLGSGSDQAKGTDPLKNHQ
                     VHRLTGGYEEGGLNLALAAQLDLSENADKTKNSTTEIAATASYRFGNAVPRISYAHGF
                     DLIERGKKGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLR
                     HKF"
BASE COUNT          498 a          444 c          503 g          498 t
ORIGIN      
        1 gccgcgtgtg ttttttatgg cgttttaaaa agccgggact gcatccgggc agcagcgcat
       61 cggcccgcac gaggtctgcg cttgaattgt gttgtagaaa cacaacggtt tttgaaaaaa
      121 taagctattg ttttatatca aaatataatc atttttaaaa taaaggttgc ggcatttatc
      181 agatatttgt tctgaaaaat ggttttttgc gggggggggg tataattgaa gacgtatcgg
      241 gtgtttgccc gatgttttta ggtttttatc aaatttacaa aaggaagccg atatgcgaaa
      301 aaaacttacc gccctcgtat tgtccgcact gccgcttgcg gccgttgccg atgtcagcct
      361 gtacggcgaa atcaaagccg gcgtggaagg caggaactac cagctgcaat tgactgaagc
      421 acaagccgct aacggtggag cgggagcgag cggtcaggta aaagttacta aagttactaa
      481 ggccaaaagc cgcatcagga cgaaaatcag cgatttcggc tcgtttatcg gctttaaggg
      541 gagcgaggat ttgggcgaag ggctgaaggc tgtttggcag cttgagcaag acgtatccgt
      601 tgccggcggc ggcgcgaccc agtggggcaa cagggaatcc tttatcggct tggcaggcga
      661 attcggtacg ctgcgcgccg gtcgcgttgc gaatcagttt gacgatgcca gccaagccat
      721 tgatccttgg gacagcaata atgatgtggc ttcgcaattg ggtattttca aacgccacga
      781 cgatatgtcg gtttccgtac gctacgattc ccccgaattt tccggtttta gcggcagcgt
      841 ccaattcgtt ccggcccaaa acagcaagtc cgcctatacg ccggctcatt ttgttcagaa
      901 taagcaaaat cagcggccta ctctcgttcc ggctgttgtc ggcaagccgg ggtcggatgt
      961 gtattatgcc ggtctgaatt acaaaaatgg cggttttgcc gggaactatg cctttaaata
     1021 cgcgaaacac gccaatgtgg gccgtgatgc ttttgagttg ttcttgctcg gcagcgggag
     1081 tgatcaagcc aaaggtaccg atcccttgaa aaaccatcag gtacaccgcc tgacgggcgg
     1141 ctatgaggaa ggcggcttga atctcgcctt ggcggcccag ttggatttgt ctgaaaatgc
     1201 cgacaaaacc aaaaacagta cgaccgaaat tgccgccact gcttcctacc gcttcggtaa
     1261 tgcagttcca cgcatcagct atgcccatgg tttcgacttg atcgaacgcg gtaaaaaagg
     1321 cgaaaatacc agctacgatc aaatcatcgc cggcgttgat tatgattttt ccaaacgcac
     1381 ttccgccatc gtgtctggcg cttggctgaa acgcaatacc ggcatcggca actacactca
     1441 aattaatgcc gcctccgtcg gtttgcgcca caaattctaa atagcggggc ggtgaagcgg
     1501 atagctttgt ttttgacggc ttgccttcat tctttgattg caatctgact gccaatctgc
     1561 ttcagcccca aacaaaaatc cggatacgga agaaaaacgg caataaagac agcaaatacc
     1621 gtctgaaaga ttttcagacg gtatttcgca tttttggctt ggtttgcaca tatagtgggg
     1681 gactgttagt ttttgcacaa ggaacaaata gagtaaaaaa acgctgaaat ctcggaaaga
     1741 cgtggatttc ggcgtttttt tgcatccgga aaagttacgc cagctttttc acgaaatcgc
     1801 gccggaatgc gcggttttct gtttaaagct gacgagatta gggaattttt aaaactgttt
     1861 taagaggttt ttaaaatgga tttaatcaat actccggcca taccactcaa cctgtgttgc
     1921 cgctttgacg aactgagcat ccc
//
LOCUS       DAWFLT010000152         1935 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWFLT010000152 DAWFLT010000000
VERSION     DAWFLT010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.152"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            389..1807
                     /locus_tag="WGR74_002114"
     CDS             389..1807
                     /locus_tag="WGR74_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ0906440.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAMFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAACAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
BASE COUNT          419 a          622 c          450 g          444 t
ORIGIN      
        1 aaattataac ccccttcctg ccgacgcacg cactttccgc gcggcgcatt ccccttttcc
       61 cgcccctcaa atccgccttt tcttcaggca gggtttcagc ccgcctcttt tccctgtttt
      121 cctttccccg acacgcgtgc gcgccccctg ccgcactgtg ctgcactttc gcgcccggac
      181 ggcatcgttc cgccatccgg ttctctgttt taaatacccc tgtttcagaa agaaatgcag
      241 atgtttcaac acacaggacg acacataaag caccgcccta tgtgttgccc tgatttggaa
      301 ggggttacgc ctcccaaata aagtctgatc ctgccgcccc gaaggacaga tgtccgagtg
      361 gcgaagtttc aaccgaaaag gaaatacgat gaatattcac accctgctct ccaaacaatg
      421 gacgctgccg ccattcctgc cgaaacggct gctgctgtcc ctgctgatac tgctggcccc
      481 caatgcaatg ttttgggttt tggcactgct gaccgccacc gcccgcccga ttgtcaattt
      541 ggactatctt cccgccgcgc tgctgatcgc cctgccttgg cgtttcgtca aaattgccgg
      601 cgtattggcg ttttggctgg cggttttgtt tgacgggctg atgatggtga tccagctctt
      661 cccttttatg gacctcatcg gcgccatcaa cctcgtcccc ttcatcctga ccgcccccgc
      721 cccttatcag ataatgaccg ggctgttgct gctgtatatg ctggcgatgc cgtttgtgtt
      781 gcagaaagcc gccgccaaaa ccgacttccg acacattgcc gcctgtgccg ccgttgtggc
      841 ggcagccggc tatttcactg gccatttgag ttactacgac cgggggcgga tggccaatat
      901 cttcggcgca aacaacttct actacgccaa aagtcaggcg atgctctaca ccgtcagcca
      961 gaatgccgac tttattaccg ccggcctggt cgatcccgtc ttcctcccct tgggcaatca
     1021 acagcgtgcc gccacgcatc tgaacgagcc gaaatctcaa aaaatcctct ttatcgtcgc
     1081 cgaatcttgg gggctgccgg ccaatcccga acttcaaaac gccacttttg ccaaactgct
     1141 ggcgcaaaaa gaccgttttt cggtttggga aagcggcagt tttcccttta tcggcgcgac
     1201 ggtcgaaggc gaaatgcgcg aactgtgtgc ctacggcggt ttgcgcgggt tcgcactgcg
     1261 ccgcgcgccc gacgaaaaat ttgcccgctg cctccccaac cgtttgaaac aagaaggtta
     1321 cgccaccttt gcgatgcacg gcgcgggcag ttcgctttac gaccgcttca gctggtatcc
     1381 gagggcgggc tttcaagaaa tcaaaaccgc cgaaaacctg atcggtaaaa aaacctgcgc
     1441 cattttcggc ggcgtgtgcg acagcgagct gttcggcgaa gtgtcggcat ttttcaaaaa
     1501 acacgacaag ggactgtttt actggatgac gctgaccagc cacgccgact atcccgaatc
     1561 cgacattttc aaccacaggc tcaaatgcac cgaatacggc ctgcccgccg aaaccgacct
     1621 ctgccgcaat ttcagcctgc acacccaatt cttcgaccaa ctggcggatt tgatccaacg
     1681 ccccgaaatg aaaggcacgg aagtcatcat cgtcggcgac catccgccgc ccgtcggcaa
     1741 cctcaatgaa accttccgct acctcaaaca ggggcacgtc gcctggctgc acttcaaaat
     1801 caaataacaa caatgccgtc tgaacgcacc aacagccttc agacggcatt ttgcagacag
     1861 accgaccctt caagcccact tttttcatca tctccgataa attgctttgt atagtggatt
     1921 aacaaaaacc agtac
//
LOCUS       DAWFLT010000153         1823 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWFLT010000153 DAWFLT010000000
VERSION     DAWFLT010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.153"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            309..980
                     /locus_tag="WGR74_002115"
     CDS             309..980
                     /locus_tag="WGR74_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0906441.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
     gene            1042..>1823
                     /locus_tag="WGR74_002116"
     CDS             1042..>1823
                     /locus_tag="WGR74_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0906442.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKNR
                     YLRDHRGRPAYPLLSMFKAVLPGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSELLELINCQLTEKGLKVEKASAAVIDATIIQTAGSEQRQAIE
                     VDEEGQVSGQTTLSKDKNARWTKKNSLYKLGYKQHTRTDAEGYIEKLYITPTNAHECT
                     HLSPLLEGLPKGTTVYADKGYDSKENRQHLKEHRLQNGIMRKA"
BASE COUNT          526 a          465 c          410 g          422 t
ORIGIN      
        1 ggtttgttga aagaaggtgc tcatgagaaa tcccctaaat gtcttggtgg gaatttaggg
       61 gattttgggg aattttgcaa aggtctcaaa cggatgaaat caaccgatat tcaaacacag
      121 tcatttttag cgcattttca gcgtatcgtt aatgcggaaa atttcgtgaa caggtttttt
      181 gcacaggtct ccgcctcgtt tcgcgggatg ggaaaccgta ttagaaaacg gacgcacgca
      241 acataaaccc cgaaagtgat gataagatga tgatttaacg tactgcttta attatttaag
      301 gaattatcgt gtttcccgac aaatacaagt tgagtttgga agaaaatatt tttctggcaa
      361 agaaagtatt ggttgcccaa attcacaacc tcagccgttt tgagaattgt cagacgacct
      421 tgttgcagac cgaacaaatt atccatggca aaaatgtagc ctccgcgtca ctggaagaca
      481 tccaaaccat cttgaacctg aaacgtgcct atcaatatgt gatttcgcat atttcaaacg
      541 gcgaaccggt cgatatttca ctccttaaaa aaatcaacaa cattgttgcc aaggacgatt
      601 ctttggcacc cggtgatttc cgtaccggtt cggtcggcgt aacgctattg gacggttccc
      661 gtcatgcccc aaatccggtg aaggaaattg aagtggctcg cgtgttacaa aatatcggac
      721 tgcaaagcgg ttcgacgacg gaggcagccg tccgttttat gctttattgt atgcggcagc
      781 aggttttttg ggacggcaac aaacgaacgg caaccttatt tgccaacggt ctgatgatgg
      841 cggggggctg cggcatcttg gaaatctccg aaatgcagat gccgcaattc aatgaaaaac
      901 tgtccgcatt ctatcgctcc ggcgacgata ccgatatttc caagtttgtg tatcaaaatt
      961 gtatatcggg catagactga gacctttgca aaattcccca aaatccccta aattcccacc
     1021 aagacattta ggggatttct catgagcacc ttcttccggc aaaccgccca agccatgatt
     1081 gccaaacaca tcgaccgctt cccactattg aagttggatc aggtaattga ttggcagccg
     1141 atcgagcagt acctgaaccg tcaaaaaaac cgttacctcc gagaccaccg cggccgtcct
     1201 gcctatcccc tgctgtccat gttcaaagcc gtcctgcccg gacaatggca cagcctctcc
     1261 gatcccgaac tcgaacacag cctcatcacc cgcatcgatt tcaacctgtt ttgccgtttt
     1321 gacgaactga gcatccccga ttacagcacc ttatgccgct accgcaactg gctggcgcaa
     1381 gacgacaccc tgtccgaact gttggaactg attaactgcc aactgaccga aaaaggctta
     1441 aaagtagaga aagcatccgc cgccgtcatt gacgccacca ttattcagac cgccggcagc
     1501 gaacagcgtc aggccataga agtcgatgaa gagggacaag tcagcggtca aaccacactg
     1561 agtaaggaca aaaatgcccg ttggacaaag aaaaacagcc tctacaaact cggttacaaa
     1621 caacataccc gtaccgatgc ggaaggctat atcgagaaac tgtacatcac tcccaccaat
     1681 gcccatgagt gcacacacct gtcgccgttg ttggaaggac tgcccaaagg tacgaccgtc
     1741 tatgccgaca aaggctacga cagcaaggaa aaccggcaac atctgaaaga acatcggttg
     1801 cagaacggca ttatgcgcaa agc
//
LOCUS       DAWFLT010000154         1754 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWFLT010000154 DAWFLT010000000
VERSION     DAWFLT010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.154"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(395..1384)
                     /gene="porB"
                     /locus_tag="WGR74_002117"
     CDS             complement(395..1384)
                     /gene="porB"
                     /locus_tag="WGR74_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HEZ0906443.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSLDYQDGQV
                     YSVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGGAYKRHQDVDDVKIEKYQIHRLVSG
                     YDNDALYASVAVQQQDAKLVEDNSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSVDD
                     AKRDNTYDQVVVGAEYDFSKRTSALVSAGWLQEGKGENKFVATAGGVGLRHKF"
BASE COUNT          447 a          388 c          451 g          468 t
ORIGIN      
        1 atgtaggttc gtaggaatga cgtgatgcag gtttccgtat ggatggattc gtcattcccg
       61 cgcaggcggg aatctaggtc tgtcggtgcg gaaacttatc ggataaaacg gtttcttgag
      121 attttacgtt ctggattccc actttcgtgg gaatgacggg attaaagttt caaaatttat
      181 tctaaatagc tgaaactcaa cgcactagat tcccgcctgc gcgggaatga cggcatattt
      241 tgacattgaa taaaaaagac taaaaacaga aaacagccaa acagaaaaca gcgaaacagg
      301 aaaaagccaa acagaaaaaa gccaaacaga aaaaagccaa acagaaaaaa gcctgtctgg
      361 cgacaggctt tttgttgata ccaatctttg cagattagaa tttgtggcgc agaccgacac
      421 cgccggcagt cgctacgaat ttgttttcgc ctttgccttc ttgcaaccaa ccggcagaaa
      481 ccaaggcaga agtgcgtttg gagaagtcgt attccgcacc gacaaccact tggtcgtaag
      541 tattgtcgcg ttttgcatca tcaaccgagc ctttgaagcc gtgggcgtaa gaaacgcggg
      601 gcgttacgtt gccgaagcgg tatgccaagg tagcggcaac ttcggtttga gagttgtgcg
      661 aattgtcttc aaccagtttc gcgtcttgtt gctgtacggc tacggaagcg tatagggcat
      721 cattgtcgta accgctgacc aaacggtgaa tctggtattt ctcaatcttc acgtcatcca
      781 catcctgatg tcttttatag gcaccgccat attgcacgaa gaagccgccg tttttgtagt
      841 tgaagccggc gtggtaagat tcgctgttat gtctgcctgc attgtcgtta agcgcgtatt
      901 gtacgctgcc gctgaggccg gcaaattcgg gagaatcgta gcgtacggaa atgaggcgtg
      961 cctcgggttc ggcaattttg tttacaccca aatagtcgct tttgctatcc caaggattga
     1021 tgtcgccggt gtctttcagg acgctgttca aacggccgac gcgcaattta ccgaagccgc
     1081 ctttcaagcc gatgaaggat tggcggttgc cccaaccgga gtcagtaccg gcgatagatg
     1141 ctttttgctc aacctgccaa atggctttca ggccgttacc gaggtcttct tggcctttga
     1201 agccgatttt cgaacccaaa tcaacgatgc cggtaccggt ttcaacgcta taaacttgac
     1261 cgtcctggta atctagggag cgggaagttt ctacgccggc tttgatggtg ccgtacaggg
     1321 taacgtcagc cattgctgca acaggaaggg ctgccaaagt cagggcaatc agggattttt
     1381 tcattgctgt attccttttt tggttaagaa atttaagcag gccgggcttt ccaagccgct
     1441 tagctttgca tttaccgccg aggggctcgg cgattacatg tctgaaacta tagatgttcc
     1501 tgtctgaaaa aacaaacttc tgcctgtttt tgtgtggaat tcgatgtgca ttttgaagga
     1561 cggatagaga tattatgggt attttttgtt ttataacata tggttattta aattttttaa
     1621 gatttgcatt tttacaacac ttactcggga gggtattgga gggcattgca aaccgggggt
     1681 tataaagacg gcaaaaaacg cacacgcggt ttctttcgga acgggcggat gcaaaacccg
     1741 cccctgcggc agga
//
LOCUS       DAWFLT010000155         1741 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWFLT010000155 DAWFLT010000000
VERSION     DAWFLT010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.155"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(232..1155)
                     /locus_tag="WGR74_002118"
     CDS             complement(232..1155)
                     /locus_tag="WGR74_002118"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ0906444.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRNCG"
BASE COUNT          472 a          402 c          412 g          455 t
ORIGIN      
        1 agattgcggc atttatcgga aaaaacagaa accgctctgc cgtcattccc gcgcaggcgg
       61 gaatctagac cttagaacaa cagcaatatt caaagattat ctgaaagtcc gagattctag
      121 attcccactt tcgtgggaat gacgaatttt aggtttctgt tttggttttt tgtccttgta
      181 ggaatgatga aattttaagt tttaggaatt taccggaaaa aacagaaagc gttatccaca
      241 atttctgatg ttcagctcgt gaaatgcgtc gggcaaatca tcgctgtcgg caaattccac
      301 ccggtcgtaa gccgtttcgt ctgccaaaac cgcgcgcaag agtgcgttgt tgatggcgtg
      361 tcccgatttg tagccttcaa acgcgccgac aatcggatgt ccgacgatat acaaatcgcc
      421 gatggcatca aggattttgt ggcgcacaaa ctcatcggga tagcgcaagc cttcaggatt
      481 caggacatcc gtgtcgtcaa tcacgatggc gttgttcaaa ttgccgccca aacccagatt
      541 gtgggcgcgc atcatttcca cttcgtgcat aaagccgaag gtgcgcgcgc gcgcgatttc
      601 gtcgatgtag gatttgccgg cgaaatcaat ttcaaaagtg ggcgagctgc ggttgaaaac
      661 cggatggtcg aattcgatgg tcagcgttac cttaaagccg tcatacggcg taaagcgtac
      721 ccatttgccc gcttctttga tttcgacagg cttgaggatt ttcaaaaaac gcttttgcgc
      781 cttttgatcg accacgcccg catcttgcaa aaggtaaata aacggcaggc tggagccgtc
      841 cataatcggg atttcgggcg cgttcagctc aatcagcgca ttgtcgatgc cgtaggcgga
      901 cagcgcggac ataatgtgtt cgattgtgcc gacgcgcacg cctttgtcgg taacgatggt
      961 ggaggaaagg cgggtatcgt tgatcaaata aggggtcagt ttgatttgtt cgcccatctc
     1021 gccgtccaaa tcggtacggc ggaaggaaat cccgctgttt tcaggcgcgg ggtgcagggt
     1081 cagcgcgacg cgttcgcccg aatgcagccc gactccggta acgctgatgg atttcgccaa
     1141 agttctttgc agcataaacc gcttccttat caatggggta agttttggaa taatacgata
     1201 aaaccggaaa aacaagctat gtttttccat agtatttgcc aatgtatctg ttttcaatac
     1261 gtaagccgca taaaaatgta tagtggatta acaaaaatca agacaaggcg acgaagccgc
     1321 ccctctcgaa aaacgcaaaa aatgccgtcc gaaaaccttt cggacagcat tttcgcgtga
     1381 accgtcattc ccacaaggac aaaaaaccaa aacataaaac caaaaacaga aacctaaaat
     1441 tcatcattcc cacgaaagtg ggaatctagt tcgttgagtt tcagttattt agaataaatt
     1501 ttgaaactta aatcccgtca ttcccacgaa cctacattcc gtcattccca cgaaagtggg
     1561 aatccagttt tttgagtttc agtcattccc gataaattgc cttagcattg aatgtctaga
     1621 ttcccgcttt tgcgggaatg acggcggcgg gttgctgttt tttgtttata aatttctgta
     1681 gctttgaatt tcagccatct tcaataaatt atagcggatt aacaaaaacc agtacggcgt
     1741 t
//
LOCUS       DAWFLT010000156         1731 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWFLT010000156 DAWFLT010000000
VERSION     DAWFLT010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.156"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            357..1661
                     /locus_tag="WGR74_002119"
     CDS             357..1661
                     /locus_tag="WGR74_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ0906445.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          352 a          434 c          513 g          432 t
ORIGIN      
        1 caattaaaaa tttttaatat attaatcaat aaattaattt tataaaataa aaatattgtc
       61 aacaatattt tgccttatcg cccaaacctc tgtatatttt cctacagtaa attgttgaca
      121 atccatacgc ccacatatgc gccgcctaag gataaatcct cccgccggac aacgggtgca
      181 agggattgga tgcgatattt ccatattcaa acaagggatt tgtttcacgc acagggcggc
      241 acatcggcaa aatccccgcg ccgcgccggt ccggcagggc ttgcgtccct cccggacaag
      301 ccccgacccc gcctttccga aagacgggct caaccattaa ggaaacttta atcaaaatga
      361 aaaaacacat atgggcggca tctttgctgc cggcatccct atcggcagaa cctttaaact
      421 ggtggaagcc ttattccgcc gtcaattcgg gcgataccgc ctgggtgatg actgcggctg
      481 ccttggtact gttgatgacg cttcccgggc tggctttatt ctacggcggt atggtgcgga
      541 aaaaaaacct gctctcgacg atgatgcaca gcttttccat cgcgacattg gtgggcatcc
      601 tttgggtcgc cgtcggctat tctttagcgt tcacgccggg aaatgccttt atcggcggtt
      661 tggggcgcgt atttttaagc gggatgcaga tagacgctgc cgcacagatg ctgaccgtgt
      721 cgcccaatgc gccgactgtt cccgaaccgg tatttatgtt ttttcagatg acgtttgcca
      781 ttatttcgac cgccattatt accggcgcgt ttgccgaacg gatgaaatat tcggcaatga
      841 tgctgttttc gggcatatgg tttttattgg tttatgtgcc gggcgcgcat tgggtgtggg
      901 gcggcggctt tatgagcaag ggcggcgtat tggattatgc cggcggtacg gtggtgcaca
      961 tcaatgccgg tatcgcggga ctcgtcgccg ccttggtttt gggcaggcgc ataggctacg
     1021 ggcgcgaggc gatgcctccg cacaatatgg cgatgacact gatcggcgcg gcaatgttgt
     1081 ggttcggctg gttcggcttt aacgccggat cggcgcttgc ggcagacgcg gcggcgggta
     1141 tggcgatggc ggtaacgcag gtgtcggccg tattcggcgc ggcaggctgg cttgcctgcg
     1201 aaaaaatagc gggacacaaa ccttccgctt tggggctggc ttccggcgcg gtttccggtc
     1261 tggtcggcat cacccctgcc gccggcttta ccggcccgtc gggcgcggcc gccatcggta
     1321 tattgactgc cgccgcgtgc tttgtgtccg tcaccgtcgt caaacacaaa ttgcgttacg
     1381 atgattcttt ggacgctttc ggcatacacg gattcggcgg gctggtgggc ggaatattga
     1441 ccggcatctt tttcgacaac cgcattttcg gcggggatgc ggcagtttgg cagcagttgt
     1501 ggatacaggt aaaagacggg ttcgttatgg cggcatacag cgggctgatg agttgggcga
     1561 ttttgaaggt cgtggggaaa atctgcggcg gcctgcgcgt cggcaaggat gtcgaacgcg
     1621 aaggtttgga tctgaatatc cacggcgaac gcgtggaata agggcggcta tgccgtctga
     1681 agcctgaaaa tcgggtttca gacggcattt ttcacgtttg ccgccgatgg a
//
LOCUS       DAWFLT010000157         1647 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWFLT010000157 DAWFLT010000000
VERSION     DAWFLT010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.157"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..876
                     /locus_tag="WGR74_002120"
     CDS             <1..876
                     /locus_tag="WGR74_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HEZ0906446.1"
                     /translation="TPTNAKKHQPQKPDKTALEKIIQGIMPAHAAGAMTNPQDKDAAI
                     WISNIRNGITGPIVITSYGVYAAGWTAPLIGTAGKLAISTCMANPSGCTVMVTQAAEA
                     GAGIATGAVTVGNAWEAPVGALSKAKAAKQAAPKETINNLANLAKAEQQILFRIAQRD
                     TQLDAWKTGFNNRVRKGAGLLDASNIPITINGKTIKPVQAISLKGAPVYSGVSEQEIF
                     ALYRQMTGQNPNFRVLPDGRLANGIISTGEWAGTKIALRNFSKTENSTQARWTLDLQN
                     PPSFIKGTKLELKFQ"
     gene            895..1269
                     /locus_tag="WGR74_002121"
     CDS             895..1269
                     /locus_tag="WGR74_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ0906447.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDKNLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
BASE COUNT          516 a          340 c          369 g          422 t
ORIGIN      
        1 acccctacca atgcgaaaaa gcatcaaccg cagaagcccg acaaaaccgc actggaaaaa
       61 attatccaag gtattatgcc tgcacatgca gcaggtgcga tgactaatcc gcaggataag
      121 gatgctgcca tttggataag caatatccgt aatggcatca caggcccgat tgtgattacc
      181 agctatgggg tttatgctgc aggttggaca gctccgctga tcggtacagc gggtaaatta
      241 gctatcagca cctgcatggc taatccttct ggttgtactg tcatggtcac tcaggctgcc
      301 gaagcgggcg cgggaatcgc cacgggtgcg gtaacggtag gcaacgcttg ggaagcgcct
      361 gtgggggcgt tgtcgaaagc gaaggcggct aagcaagctg ctcctaaaga aacaataaac
      421 aatttggcaa atttagccaa agcagaacag cagattttat tccgtattgc ccaacgcgat
      481 acgcaactgg atgcatggaa gacgggattt aacaatagag taaggaaagg agcaggcttg
      541 cttgatgcaa gtaatattcc gataaccatt aacggaaaaa ccatcaaacc tgtacaagcc
      601 ataagcttaa agggagcacc cgtttacagc ggcgtaagcg aacaggagat ttttgcgctt
      661 tatcggcaga tgactggcca gaatccgaat tttagagttt tgcctgacgg aagattagca
      721 aatggcatta tcagtactgg agaatgggca ggaacaaaaa ttgcattaag aaatttttca
      781 aaaacagaga attcaactca agcacgatgg acattagatt tgcagaatcc tccatcattt
      841 attaaaggta ctaaattgga gcttaaattc caataattta caaaggattt taccgtggat
      901 gagaaacaaa aaattaagat tcttgatttt caaatcgatt tatcctcaat ttttaactct
      961 tataaaaatc aaatgggtat taatattcaa gataaaaact taaaaaacaa tttctgttct
     1021 tttatggaag aactgttaaa tgacggttca atccgtttac atgattatac cgacggtatc
     1081 ggaattcctc taactggaac ttcaaaagaa caagtgcaga aattgaaaga catatggcct
     1141 actttggaag atgcccaagc aatatggcct gaagaccctt ggtattactt agaatggctt
     1201 tggtgggata ttgcgtgtcc aatagatttg gccgatttgc cgaatattga tatttatgag
     1261 caagcgtagg tatggttagc cgcctttagc ggcgtaaccg tacgcatatc agcaaacttt
     1321 ataaaataac aaggccgtct gaaatctgtt tttcaacttt ttcagacggc cttgcaactt
     1381 ggcatttcat tcgtacggtt acgccgctaa aggcggctaa ccgtacctac gagctctgat
     1441 aaaaatgatt tatggaagca agctgtagcc tgcatgaaac ctaaaatcca tgcgtaaggt
     1501 gtgtgctcca gcacgcacgc gttccatgat ttacggctca atgccgtctg aaaagctcac
     1561 aatttttcag acggcatttg ttatgcaagt aaatattcag attccctata tactgcccag
     1621 acgcgtgcgt gctgaagaca cccccta
//
LOCUS       DAWFLT010000158         1612 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWFLT010000158 DAWFLT010000000
VERSION     DAWFLT010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.158"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..825
                     /locus_tag="WGR74_002122"
     CDS             <1..825
                     /locus_tag="WGR74_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HEZ0906448.1"
                     /translation="TPTNAKKHQPQKPDKTALEKIIQSIMPAHAAGAMTNPQDKDAAI
                     WISNIRNGITGPIVITSYGVYAAGWTAPLIGTAGKAAISTCMANPSGCTVMVTQAAEA
                     GAGIATGAVTVGNAWEAPVGALSKAKAAKQAIPTQTVKELDGLLQESKNIGAVNTRIN
                     IANSTTRYTPMRQTGQPVSAGFEHVLEGHFHRPIANNRSVFTISPNELKVILQSNKVV
                     SSPVSMTPDGQYMRTVDVGKVIGTTSIKEGGQPTTTIKVFTDKSGNLITTYPVKGN"
     gene            832..1341
                     /locus_tag="WGR74_002123"
     CDS             832..1341
                     /locus_tag="WGR74_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906449.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
BASE COUNT          517 a          321 c          325 g          449 t
ORIGIN      
        1 acccctacca atgcgaaaaa gcatcaaccg cagaagcccg acaaaaccgc actggaaaaa
       61 atcatccaaa gtattatgcc tgcacatgcg gcaggtgcga tgactaatcc gcaggataag
      121 gatgctgcca tttggataag caatatccgt aatggcatca caggcccgat tgtgattacc
      181 agctatggag tttatgcagc aggttggaca gctccgctga tcggtacagc tggtaaagca
      241 gctatcagca cctgtatggc taatccttct ggttgtactg tcatggttac gcaggctgct
      301 gaagcgggcg cgggaatcgc cacgggtgcg gtaacggtag gcaacgcttg ggaagctcca
      361 gtaggagcgt tgtcgaaagc gaaggcggct aagcaggcta taccaaccca gacagttaaa
      421 gaacttgatg gcttactaca agaatcaaaa aatataggtg ctgtaaatac acgaattaat
      481 atagcgaata gtactactcg atatacacca atgagacaaa cgggacaacc ggtatctgct
      541 ggctttgagc atgttcttga ggggcacttc cataggccta ttgcgaataa ccgttcagtt
      601 tttaccatct ccccaaatga attgaaggtt atacttcaaa gtaataaagt agtttcttct
      661 cccgtatcga tgactcctga tggccaatat atgcggactg tcgatgtagg aaaagttatt
      721 ggtactactt ctattaaaga aggtggacaa cccacaacta caattaaagt atttacagat
      781 aagtcaggaa atttgattac tacataccca gtaaaaggaa actaactaaa tatgagtaac
      841 tttgaaaaaa aatatatttt agaattaaat gatgctttaa gccatttaaa tcataactct
      901 acctcatttg atttattgaa agttttgatt tcatggttat caaacgatat tgtcattgat
      961 aaatttaaaa ttttaggtta tgactttagt aaatatatcg aaatgaatcc cgatgactat
     1021 ccggttgaaa aatctatatt gaatagagag gaaattattt atctcaaaaa caatatttat
     1081 cgtaaaatat catcaggaaa ttttaaattt caatactttg tacaatatat tagagatatt
     1141 ttagaatatt tatttattga acatattgaa agagtctgtc cttactgcga atggggtgaa
     1201 atgcaaaaat tagaagaaca aaatacgcat gaaacggtgt atctctgtac tcaatgtgga
     1261 tgtgcttttt ataacgataa ttcacaattt ttattaaaaa cccctttaac cattccaatg
     1321 aaacgtgatg aatttaaata aacaagccgt agcctgcatg aaccctaaaa tccacgtgta
     1381 gcgtgtgtgc gccagcacgc atgcgttcca tgatttacgg ctcaatgccg tctgaaaagc
     1441 tcacaatttt tcagacggca tttgttatgc aagtaaatat tcagattccc tgtatgctgt
     1501 acagacgcgg gagtgttaag ccccccttgt ttgaagctcc gcggctcctg ccgagcttca
     1561 ccgaccccgt tgtgcccaag ctctctgctc ccggcggcta cattgtcgac at
//
LOCUS       DAWFLT010000159         1397 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWFLT010000159 DAWFLT010000000
VERSION     DAWFLT010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.159"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..871
                     /gene="rfbA"
                     /locus_tag="WGR74_002124"
     CDS             <1..871
                     /gene="rfbA"
                     /locus_tag="WGR74_002124"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ0906450.1"
                     /translation="GGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLAGIRDILV
                     ITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNVCLVLGDN
                     IFYGQSFTQTLKQAAAQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEE
                     KPQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEISDLNQMYLEDGSLSVQILGR
                     GFAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAK
                     NQYGQYLLRLLKK"
     gene            925..>1397
                     /gene="rfbC"
                     /locus_tag="WGR74_002125"
     CDS             925..>1397
                     /gene="rfbC"
                     /locus_tag="WGR74_002125"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ0906451.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNQCQLWIPEGFAHGFCVLGDAAEVVYKCTDYYKPEAEQVLIWNDPTVGIDWPL"
BASE COUNT          355 a          385 c          357 g          300 t
ORIGIN      
        1 cggcggcagc ggcacgcgcc tctaccccat cacgcgcggc gtatccaaac agctcctgcc
       61 cgtgtacgac aaaccgatga tttattaccc cttgtcggtt ttgatgctgg caggaatccg
      121 cgatattttg gtgattaccg cgcctgaaga caacgcctct ttcaaacgcc tgcttggcga
      181 cggcagcgat ttcggcattt ccatcagtta tgccgtgcaa cccagtccgg acggcttggc
      241 acaggcattt atcatcggcg aagaatttat cggcaacgac aatgtttgct tggttttggg
      301 cgacaatatt ttttacggtc agtcgtttac gcaaacattg aaacaggcgg cagcgcaaac
      361 attgaaacag gcggcagcgc aaacgcacgg cgcaaccgtg tttgcttatc aggtcaaaaa
      421 ccccgaacgt ttcggcgtgg ttgaatttaa cgaaaacttc cgcgccgttt ccatcgaaga
      481 aaaaccgcaa cggcccaaat ccgattgggc ggtaaccggc ttgtatttct acgacaaccg
      541 cgccgtcgag ttcgccaaac agctcaaacc gtccgcacgc ggcgaattgg aaatttccga
      601 cctcaaccaa atgtatttgg aagacggttc attgtccgtc caaatattag gacgcggttt
      661 cgcgtggctg gacaccggta cgcacgaaag cctgcacgag gcggcttcgt tcgtccaaac
      721 cgtgcaaaac atccaaaacc tgcacatcgc ctgcctggaa gaaatcgctt ggcgcaacgg
      781 ttggctttcc gatgaaaaac tggaagaatt ggcgcgcccg atggcgaaaa accaatacgg
      841 ccaatatttg ctgcgcctgt tgaaaaaata atgtttgagg ccgtctgaaa cttttcagac
      901 ggcctttaga tgaaagataa aaagatgaac atcattgata ccgccattcc tgacgtaaaa
      961 ctgcttgagc cccaagtctt cggcgacgcg cgcggctttt ttatggaaac cttccgcgac
     1021 gagtggttta aaacccaagt ctgcgaacgc accttcgtgc aggaaaacca ctccaaatcc
     1081 ggcaaaggcg tattgcgcgg cctgcactac caaaccgaaa acacacaagg caaactcgta
     1141 cgcgtagttg tcggcgaagt gttcgacgtg gccgtcgata tgcgcgaagg ttcgccgact
     1201 ttcggcaaat gggcgggcgc aaccctgtcc gcgcaaaacc aatgccagct ttggataccc
     1261 gaaggcttcg cgcacgggtt ttgcgttttg ggcgacgcgg cggaagtcgt gtacaaatgc
     1321 accgactatt acaagcctga agccgaacag gttttaatat ggaacgaccc gacagtcggc
     1381 atcgactggc cgcttca
//
LOCUS       DAWFLT010000160         1389 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWFLT010000160 DAWFLT010000000
VERSION     DAWFLT010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.160"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..240
                     /locus_tag="WGR74_002126"
     CDS             <1..240
                     /locus_tag="WGR74_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0906452.1"
                     /translation="VLSKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPF
                     FISNDTDLALFKTTKWGICRAIADAIAMELGAARV"
     gene            237..443
                     /locus_tag="WGR74_002127"
     CDS             237..443
                     /locus_tag="WGR74_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906453.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNRR"
     gene            446..610
                     /locus_tag="WGR74_002128"
     CDS             446..610
                     /locus_tag="WGR74_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906454.1"
                     /translation="MGQVAFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
     gene            613..849
                     /locus_tag="WGR74_002129"
     CDS             613..849
                     /locus_tag="WGR74_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906455.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTVKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSLVSVKRQEVENESRETAGE"
     gene            821..1240
                     /locus_tag="WGR74_002130"
     CDS             821..1240
                     /locus_tag="WGR74_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906456.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTLLAVSHHQGYKSAFAKQQAVID
                     KMEKDKAQALLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNAGGGCIDGFGHHGLQLYKRALGYGN"
BASE COUNT          364 a          329 c          410 g          286 t
ORIGIN      
        1 gtgttgagca aagccgttgc caagaaaacc ggctggaaac tgcgcggcga agacggcttt
       61 aaacccgaca atgcgggcca gcattcgcgc ctggcttatg cacaagccgg cggcattgtg
      121 tttgagcctt ttttcatcag caacgacact gatttggcct tgtttaagac gactaaatgg
      181 ggcatctgcc gcgcgattgc ggacgcgatt gcgatggaat tgggggcggc aagagtatga
      241 atattattgg taaattgaaa gaagctgctt cctattttct tacaaaattg attggagaaa
      301 atcctagtaa tgagcaggta aaccgcgcac ttatacagat gccaaatgtt cgtccgatac
      361 acacctatcc acgcccaaat ttaagaaact caggcgtggc agccgcgaaa cgcgcggcgc
      421 gcaaacgcaa gaatcgtcgt taatcatggg acaggttgcg ttttacgaaa agatgattgg
      481 gctgtggtcg gccaaaagcc gtgaggcaag cgaacaggca gacttggcgg cgtttgaatt
      541 tgcggagggc gaactggcca attatcagga aatgctgaaa cggcacctgc aaaccaaaag
      601 tgtggaatag caatgcgtat tttggatatt tttaaaaacc cagcgacagg caatgtgtcg
      661 cactcgaaac tgtgggcaaa cgttgcctgc gcggcgggga cggttaagtt tgtgatgctg
      721 cccgacccgt cggcggagat ttgggcggtg tatttgggca ttgtcggcgg ctatgcggtg
      781 gcgcgttcgt tggtcagcgt caaacgtcag gaggtcgaga atgaatctcg tgaaactgct
      841 ggcgaataac tggcaaccga ttgccatcat cgcgcttgtc ggcacgctct tggcggtgtc
      901 gcaccatcaa ggctacaagt cggcttttgc gaagcagcag gcggtcattg acaagatgga
      961 aaaagacaaa gcgcaagccc tgctgttgtc ggctcaaaac tacgcccgcg agctggaaca
     1021 ggcgcgtgcg gaagctaaaa aatatgaagt caaggcgcac gccgtcggca tggctttggc
     1081 gaaaaaacag gcggaagtca gccgtctgaa aacggaaaat aaaaaggaaa tcgaaaatgt
     1141 ccttactcaa gaccgtaaaa atgcaggcgg cggttgtatt gacggctttg gccatcacgg
     1201 cttgcagctc tacaagcgcg ccctcggcta cggaaattaa ggttgtcgaa aaggcggtca
     1261 tgccgacacc gcctgccgcg ttgatggtcg cgccggtgcg cccgaatccg ccgaaagacg
     1321 gcaagacggc cacgctgttg gaacacgccg ccgagtttgg cggctatgtt gccgaacttg
     1381 aaaaccaaa
//
LOCUS       DAWFLT010000161         1166 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWFLT010000161 DAWFLT010000000
VERSION     DAWFLT010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.161"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(90..641)
                     /locus_tag="WGR74_002131"
     CDS             complement(90..641)
                     /locus_tag="WGR74_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906457.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
     gene            complement(695..1166)
                     /locus_tag="WGR74_002132"
                     /pseudo
     CDS             complement(695..1166)
                     /locus_tag="WGR74_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          337 a          371 c          219 g          239 t
ORIGIN      
        1 caaagaaacc gccattagca ctacaccccc caagctgcgg ctgccaaaca catcggctgc
       61 ttctcgacgc acccgctgaa aaatgcgtcc tatccgtccg ccttgcccgc accccctgac
      121 tttgccgccc tgcccgatgc cgtctgaagc ctgaaccgcc ccaccagccc gatgacgcac
      181 gacaccgcag gaaagggcat acacaaagcc gtcagcacat aacgcgcatt ccccgtattg
      241 accacgtccg cgcccttcag cagcagcggc agcgcgttcg cccactccca caccgacggc
      301 gcgctgaacg ccaataccgc caccagcccc aacatccgga acaccccgat ttccaacacc
      361 tgccgccgcc gcaccgaacg gttgagcaaa aacacaaccg ccaacccgaa acagaccacc
      421 gccgccatac cgccgttggc gacaatctgc aaactgttga atgcaatatc gggcgtaacc
      481 gccaacacgc cttccgaatc cggcacggca cgctgcaaat ccaaaccctg caacacattc
      541 aacacaaaac cgtagatcaa atcaaaaaac accacgccta tcggcaaagc actcaaaacc
      601 cgccacatct tcctgtccga ccaaccgttc aaaactttca ttttacccga ccacgcaagc
      661 cgccgaacaa aaaataaggg gctgtcctag ataactagga taaactcggt tttactaatt
      721 gttttaaaat ggaacaagaa cttttatctc actgttgtta aaacgccatt cgcactcctt
      781 taaatacagc tcaaaatgcg ctttgggaat gccgttaaac ttgcgtaaat gacgttttgc
      841 ctggttccaa aagttctcaa ttccattaat atggttttgt cgttcagcaa aatgtgtgct
      901 gtgattgata cgaaaacgaa gtttcagcga agctaaaatg gctaaattcg ctcacatcta
      961 atacatcata gctacgataa caatccgtat aaacaatact gtcaggtttc acttgttcac
     1021 ggataatagg aaataaagta gcggtttgag tattcggtac tgtaaccgta taaaccttac
     1081 catttcgctt caaaagaccg aatacggcga ctttaccggc agcaccgcga ccgcgtttgc
     1141 ctttgcgttg tccgccaaaa taactt
//
LOCUS       DAWFLT010000162         1146 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWFLT010000162 DAWFLT010000000
VERSION     DAWFLT010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.162"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..424)
                     /locus_tag="WGR74_002133"
     CDS             complement(<1..424)
                     /locus_tag="WGR74_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0906458.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEGSVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVNNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKLD"
     gene            complement(544..750)
                     /locus_tag="WGR74_002134"
     CDS             complement(544..750)
                     /locus_tag="WGR74_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906459.1"
                     /translation="MFDGAKVVRRVEGNLWVFGMVALEKTCFKGQMPSENRFQTAFSV
                     YLKQTGKGSNILQSSYSHKRFIVN"
     gene            1040..1146
                     /locus_tag="WGR74_002135"
                     /pseudo
     CDS             1040..1146
                     /locus_tag="WGR74_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
BASE COUNT          285 a          311 c          225 g          325 t
ORIGIN      
        1 cgtccaactt gtcgcagctg ctcagactat cggtataaac aatgctgtcc ggcatgattt
       61 tctttttgat gacagggagt aacgtttcag acttggcatt gtttacgaca acggtataga
      121 cccgtccgtt gcgtttcaga atgccgaaga caaccacttt tcctgccgca ccgcgaccac
      181 gtctgccttt acgccgtccg ccgaaatcgc tttcgtccgg ctcgacagag ccctcaaaaa
      241 cctcatcggc agccaaggct aaatgatggt tgataaccgt gcggatttta cggtagaaca
      301 gtgctgccga attgggatgg atacccaaaa tatcggcggc agaacgggcg gtaacttcga
      361 gtacaaaaaa acggagcagt tctttctgta cttttttctt taatttgcag tgcgttatct
      421 tcatatttcg agggtaacat atctgctaat ctagtacagc ccctagttta aagctttgtt
      481 tcttaagtcc gcagagtatg ccatggttag accttcaaag ttgagtattg tactattttg
      541 tttttaatta actataaaac gcttgtgtga ataggaagac tgcagaatat tgctgccttt
      601 tcctgtttgc tttaaataaa cagaaaatgc cgtctgaaac cgattttcag acggcatttg
      661 tcccttaaaa cacgtttttt caagcgcgac cataccaaac acccacaggt tgccttccac
      721 ccttcgtaca acctttgctc catcaaacat ccacttccta aatactgatg tctgcttagg
      781 ttgaatcggc attcgtacga ttacggcaca ccgcctaatc tgtacctgcg tctgttgcgc
      841 ccaataccaa agactacctg aaaatctccc aaacttttca ggtagcctta tcacgttttc
      901 gactgttacc acacgcccaa agaccgcctg aaaccctttt tcagacgttc cgctccgata
      961 ccccccaaca aaacgccgcc cgaacgcatg ttcagacggc attttctttg cgtcgattca
     1021 ttccccgata tagcgcaggt ctttttccag ccatttccct tccaaacaaa aaagcaccga
     1081 cggcggccga tgccccttcc tttacaggtt cccctatttt ttagctgcgg gcagagccgg
     1141 ttttgc
//
LOCUS       DAWFLT010000163         1074 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWFLT010000163 DAWFLT010000000
VERSION     DAWFLT010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.163"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            32..>1074
                     /gene="tuf"
                     /locus_tag="WGR74_002136"
     CDS             32..>1074
                     /gene="tuf"
                     /locus_tag="WGR74_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0906460.1"
                     /translation="MGHVDHGKTTLTAALTTILAKKFGGAAKAYDQIDNAPEEKARGI
                     TINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREH
                     ILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALK
                     ALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERG
                     IIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVL
                     AKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEGVEMV
                     MPGENVTITVELIA"
BASE COUNT          292 a          298 c          267 g          217 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgcc gctttgacta ctattttggc taaaaaattc ggcggtgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtggaatac gaaaccgaaa cccgccacta cgcacacgta gactgtccgg ggcacgccga
      241 ctacgttaaa aacatgatta ccggcgccgc acaaatggac ggtgcaatcc tggtatgttc
      301 cgcagccgac ggtcctatgc cgcaaacccg cgaacacatc ctgctggccc gtcaagtagg
      361 cgtaccttac atcatcgtgt tcatgaacaa atgcgacatg gtcgacgatg ccgagctgtt
      421 ggaactggtt gaaatggaaa tccgcgacct gctgtccagc tacgacttcc ccggcgacga
      481 ctgcccgatc gtacaaggtt ccgcactgaa agccttggaa ggcgatgccg cttacgaaga
      541 aaaaatcttc gaattggctg ctgcattgga cagctacatc ccgactcccg agcgtgccgt
      601 ggacaaacct ttcttgttgc ctatcgaaga cgtattctct atttccggtc gtggtacagt
      661 agtaaccggt cgtgtagagc gcggtatcat ccacgtcggt gacgagatcg aaatcgtcgg
      721 tctgaaagaa actcaaaaaa ccacttgtac cggtgttgaa atgttccgca aactgctgga
      781 cgaaggtcaa gcaggcgaca acgtaggcgt attgctgcgc ggtaccaaac gtgaagacgt
      841 agagcgtggt caagtattgg ctaaaccggg tacaatcact cctcacacca agttcaaagc
      901 agaagtatac gtactgagca aagaagaggg cggccgccat accccgttct tcgccaacta
      961 ccgtccccaa ttctacttcc gtaccaccga cgtaaccggc gcggttactt tggaagaagg
     1021 tgtggaaatg gtaatgccgg gcgagaacgt aaccatcacc gtagaactga ttgc
//
LOCUS       DAWFLT010000164         1053 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWFLT010000164 DAWFLT010000000
VERSION     DAWFLT010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.164"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(124..552)
                     /locus_tag="WGR74_002137"
     CDS             complement(124..552)
                     /locus_tag="WGR74_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ0906461.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KKSGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(533..967)
                     /locus_tag="WGR74_002138"
     CDS             complement(533..967)
                     /locus_tag="WGR74_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ0906462.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTDKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
BASE COUNT          268 a          315 c          188 g          282 t
ORIGIN      
        1 atttgaaaac cccattaaac cgtctttaac ccatcgtccc gaacggtaga tgtttcatcc
       61 cctgcaaacc aaaaaaaaga ccgtctgaat acagacggcc aaagcctggt cacacatcac
      121 tgcctaaaat aaagctgcct gctgtgccgc aggtcggctc atttcattga taatcgtata
      181 tcccgttcgt gaagagatgc cgtatttagg gcatagcttc gtcatcgcca taagcccgct
      241 cttcttatca atatcgcgca atttgacaaa ctcctgataa aacctatggt ttctcaactg
      301 tattaacgcc ttgccgcacc gtgggacata caattcctcg ccaccatata cctgcaacag
      361 ctcatgtgtt ttcacttcgc cgatggcttc gaccaaaatt gccaaacgct cggtgtccac
      421 cttgccctta ccaaatttaa accgcgcccc gccaatcgcc ttgaccagct gttccgtcgc
      481 tgccagtccg atgacatcca caatgtccaa cacggtatcc ggcaataaat gttcaacttt
      541 ttcgaacccc atcatcccca cccgcttttt ccgttttcct gttttccgca atctgcaacg
      601 cagcaaccag tttatgtagc tgcgtatcgt ctaaatattc gaccttatcc ttaccaaaca
      661 tccgccgcgc cattgcgtgt gcatagttcc aatgtttgcc gccgacggtc agcagggctt
      721 cgactttgtc cagcattgcc gctgatgatg tccgacgcag atgcggtttg ccgtgtgggt
      781 taccttttgc tttaggctta aatccgtgcg accgcatatc agcgacaaca gactcaagtt
      841 cagaaacatc catatccgca cacgaccgct tatccgtcac acgctccaac accgcgcgat
      901 aggtaccgtc atccaagccc agctcctttt gagcaatctt aattttcgca atcaacgccc
      961 ggcgcattat ctctccaata caacatatag tataaattag cgcattttat acaataaaat
     1021 acaatatata gtattaagcc gatgtttttt tgc
//
LOCUS       DAWFLT010000165          967 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWFLT010000165 DAWFLT010000000
VERSION     DAWFLT010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.165"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            79..>967
                     /locus_tag="WGR74_002139"
     CDS             79..>967
                     /locus_tag="WGR74_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0906463.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEHYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSELLKLINRQLTEKGLKIEKASAAVVDATIIQTAGSEQRQAIE
                     VDEEGQVSGQTTPSKDSDARWIKKNGLYKLGYKQHTRTDGEGYIEKLHITPANAHECK
                     HLSPLLEGLPEGTTVYADKGYDSAENRQHLEEHQLLDGIMRKACRNRPLSEAQTKRNR
                     YLSKTRYVVEQSFGTLHRKF"
BASE COUNT          275 a          296 c          214 g          182 t
ORIGIN      
        1 cgagaaaaac ggtttgagac ctttgcaaaa ctccccaaaa tcccctaaat tcccaccaag
       61 acatttagga gatttctcat gagcaccttc ttccaacaaa ccgcgcaagc catgattgcc
      121 aaacacatcg accgtttccc gctattgaag ttggatcagg taattgattg gcagccgatc
      181 gaacactacc tgaaccgtca aagaacccgt taccttcgag accaccgcgg ccgtcccgcc
      241 tacccgctgc tgtccatgtt caaagccgtc ctgctcggac aatggcacag cctctccgat
      301 cccgaactcg aacacagcct cattacccgc atcgacttca acctgttttg ccgtttcgac
      361 gaactgagca tccccgatta cagcacctta tgccgctacc gcaactggct ggcgcaagac
      421 gataccctgt ccgaattgct caaactgatc aaccgccaac tgaccgaaaa aggtttaaaa
      481 atagagaaag catccgccgc cgttgttgac gccaccatta ttcagaccgc cggcagcgaa
      541 cagcgtcagg ccatagaagt tgacgaagaa ggacaagtca gcggccaaac cacaccgagt
      601 aaggacagcg atgcccgttg gatcaagaaa aacggcctct acaaactcgg ttacaaacaa
      661 catacccgta ccgatgggga aggctatatc gagaaactgc acattacccc cgccaatgcc
      721 catgagtgca aacacctgtc gccgttgttg gaagggttac ccgaaggtac gaccgtctat
      781 gccgacaaag gctacgacag tgcggaaaac cggcaacatc tggaagaaca tcagttgctg
      841 gacggcatta tgcgcaaagc ctgccgcaac cgtccgctgt cggaagcgca aaccaaacgc
      901 aaccgatatt tgtcgaagac ccgttatgtg gtcgaacaaa gcttcggtac gctgcaccgt
      961 aagttcc
//
LOCUS       DAWFLT010000166          941 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWFLT010000166 DAWFLT010000000
VERSION     DAWFLT010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.166"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..747
                     /locus_tag="WGR74_002140"
     CDS             <1..747
                     /locus_tag="WGR74_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEZ0906464.1"
                     /translation="LLEAARAYWQQMPSEKREAFRFHHISTDEVYGDLGGTDDLFTET
                     APYAPSSPYAASKASSDHLVRAWQRTYGLPAIVSNCSNNYGPRQFPEKLIPLMILNAL
                     DGKPLPVYGDGMQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTIC
                     ALLEELAPEKPAGVARYEDLITFVPDRPGHDVRYAVDAAKIGRELGWKPQETFESGIR
                     KTMQWYLDNKTWWQNVLNGSYRLERLGTGK"
BASE COUNT          250 a          265 c          232 g          194 t
ORIGIN      
        1 ctgcttgaag cagcccgcgc ctactggcaa caaatgccgt ctgaaaagcg cgaagccttc
       61 cgtttccacc atatttccac cgatgaagtc tatggcgatt taggcggcac ggacgatttg
      121 tttaccgaaa ccgcgccata cgcgccgtcc agtccttatg ccgcgtctaa agcatcttcc
      181 gatcatcttg tccgcgcgtg gcaacggact tacgggctgc ccgccattgt cagcaactgt
      241 tccaataatt acgggccccg acaattcccc gaaaaactca tcccgctgat gattctgaac
      301 gcgctcgacg gcaaaccgct gcccgtttac ggcgacggta tgcaaatccg cgactggctg
      361 tttgtcgaag accacgcgcg cgcactgtat caggttgtta ccgaaggtgt tgtcggcgaa
      421 acctacaata tcggcggcca caatgaaaaa gccaatattg aagtcgtcaa aaccatctgc
      481 gccctgctgg aagaactcgc tcccgaaaaa ccggccggtg tggcacggta tgaagatttg
      541 attacctttg tcccagaccg ccccggccac gatgtgcgct acgccgtcga cgcagccaaa
      601 atcggtaggg agctgggctg gaaacctcaa gaaacattcg aatccggcat ccgcaaaaca
      661 atgcaatggt atttggacaa caaaacctgg tggcaaaatg tattgaacgg tagctatcgt
      721 ctagaacgtt taggtacggg caaatagttt tcagacggca tcccgacgca atgccgtctg
      781 aaaacctatc gcaaaggaag aaagaaaaga tgtttcaaca cacaggacgg cacataaagc
      841 gtcgccctat gtgccgtcct gatttggaag gggttacccc ctcccaaata aagtctgatt
      901 ctaccgccct aaagggcggg gtttcaaccg aaaaggaaat a
//
LOCUS       DAWFLT010000167          933 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWFLT010000167 DAWFLT010000000
VERSION     DAWFLT010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.167"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..>933
                     /locus_tag="WGR74_002141"
     CDS             <1..>933
                     /locus_tag="WGR74_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0906465.1"
                     /translation="KQPEYAYLKQLQTAKNVDWKQVQLAYDKWDYKQEGLTGAGAAIV
                     VIIVTALTYGYGAAAAGSVTAAGSSTAAAATTTAAATTTVSTAAAMQTAALASLYSQA
                     AVAIINNKGDVGKALKDLGTSDTVKQIVTSALTAGALNQMGADIAQLNSKVRTELFSS
                     TGNQTIANLGGRLATNLSNAGISAGINTAVNGGSLKDNLGNAALGALVNSFQGEAASK
                     IKTTFSDDYVAKQFAHALAGCVSGLVQGKCKDGAIGAAVGEIVAESILGGRNPATLSD
                     AEKHKVISYSKIIAGSVAALNGGDVNTAANAAEV"
BASE COUNT          270 a          226 c          255 g          182 t
ORIGIN      
        1 ccaaacagcc cgaatacgcc tacctgaaac agcttcagac ggccaagaac gtcgattgga
       61 aacaggtgca gctggcctac gacaaatggg actataaaca ggaaggcttg accggagccg
      121 gtgcagcgat tgtggtgatt attgtaaccg ctctaactta tggatacgga gcggctgcag
      181 cgggtagcgt aactgccgca ggaagtagta cagccgcagc tgcaacaacg acagcggcag
      241 caactaccac tgtttctact gcagctgcca tgcaaaccgc agctttagcc tccttgtata
      301 gccaagcagc tgtagccatc atcaataata aaggcgatgt aggcaaagca ttaaaagatc
      361 tcggcaccag tgatacggtc aagcagattg ttacttccgc actgacggcg ggtgcattaa
      421 accagatggg agcagatatt gcccaattga acagcaaggt aagaaccgaa ctgttcagca
      481 gtacgggcaa tcaaaccatt gccaaccttg gaggcagatt ggccaccaac ctcagcaatg
      541 caggtatctc agctggtatc aataccgccg ttaacggcgg cagcttgaaa gacaacttag
      601 gcaatgccgc attgggagca ttggttaata gcttccaagg ggaagccgcc agcaaaatca
      661 aaacaacctt cagcgacgat tatgttgcca aacagttcgc ccacgctttg gcgggttgtg
      721 ttagcggact ggtacaggga aaatgtaaag atggggcaat tggcgcagca gttggggaaa
      781 tcgtagctga atccatactt ggcggcagaa accctgctac actcagcgat gcagaaaagc
      841 ataaagttat cagttactcg aagattattg cgggcagcgt ggcggcactc aacggcggcg
      901 atgtgaatac tgcggcgaat gcggctgagg tag
//
LOCUS       DAWFLT010000168          852 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.168, whole genome shotgun sequence.
ACCESSION   DAWFLT010000168 DAWFLT010000000
VERSION     DAWFLT010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.168"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..318
                     /locus_tag="WGR74_002142"
     CDS             <1..318
                     /locus_tag="WGR74_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0906466.1"
                     /translation="HLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRLWQKRFWEY
                     TIRDEADFAWHFDYLHFNPVKHGYVGQISDWGFSTFHRYVKQGIYPHNWGGGNADFSI
                     GYD"
     gene            355..621
                     /locus_tag="WGR74_002143"
                     /pseudo
     CDS             355..621
                     /locus_tag="WGR74_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          220 a          181 c          233 g          218 t
ORIGIN      
        1 catcttcata ccatatggcg gctgccggac aatgattctg cttattcgga acgctggcgg
       61 caaatcaagc ggcacagcca atatttaatc ggcggcaatc tcaggctttg gcaaaaacgc
      121 ttttgggaat atactatccg cgatgaggcc gattttgcct ggcattttga ttatctgcat
      181 ttcaatccgg tcaaacatgg ctatgtagga caaatttccg attgggggtt ttctacgttt
      241 caccgttatg tcaaacaggg tatttatccg cataattggg gtggcggcaa tgcggacttt
      301 tctattggat acgattgaag taatgtcgga ttcgagaatc cgacctacgg aaaactgaaa
      361 gagcatcggc tgcaggacgg cattatgcgc aaagcctgcc gcaaccgtcc gctggcggaa
      421 gtgcaaacca aacgtaaccg atatttatcg aagacccgtt atgtggtcga gcaaagcttc
      481 ggtacgctgc accgtaagtt ccgctatgct cgggcagcct atttcgggct gctcaaagtg
      541 agtgcgcaaa gccatctgaa agcgatgtgt ttgaaccttt tgaaagccgc caacaggcta
      601 agtgcgcccg ctgccgccta aaaggcagcc cggatgcctg attatcgggt atccggggag
      661 gattaagggg gtgtttgggt agaattagga ggtatttggg gcgaaaatag acgaaaacct
      721 gtgtttgggt ttcggctgtc gggagggaaa ggaattttgc aaaggcctca agatatagtg
      781 gattaaattt aaatcagtac ggcaaggcga ggcaacgctg tactggttta aatttaatct
      841 actatatttc cg
//
LOCUS       DAWFLT010000169          852 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWFLT010000169 DAWFLT010000000
VERSION     DAWFLT010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.169"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            46..738
                     /locus_tag="WGR74_002144"
     CDS             46..738
                     /locus_tag="WGR74_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0906467.1"
                     /translation="MQADLAYAAERITHNYPEPTGADKDKISTVSDYFRNIRAHSIHP
                     RVSVGYDFGGWRIAADYASYRKWNNNKYSVNTKKVQENQGTRKDLKTENQENGSFHAA
                     SSLGLSAIYDFKLNDKFDKFKPYIGARVAYGHVKHQVHSVEKETEIVTTVPTGGAQTG
                     GTIPQGPTPKPPYHESNSISSLGLGVIAGVGFDITPKLTLDTGYRYHNWGSLENTRFK
                     THEVSLGMRYRF"
BASE COUNT          247 a          246 c          197 g          162 t
ORIGIN      
        1 gcagcgcagg cggcaagtga agacggcagc cgcagcccgt attatgtgca ggcggattta
       61 gcctacgccg ccgaacgcat tacccacaat tatccggaac caaccggtgc agacaaagac
      121 aaaataagca cagtaagcga ttatttcaga aacatccgtg cgcattccat ccacccccgg
      181 gtgtcggtcg gctacgattt cggcggctgg agaatagcgg cagattatgc cagttacaga
      241 aaatggaaca acaataaata ttccgtcaac acaaaaaagg tgcaagaaaa ccaaggcacc
      301 agaaaagacc tgaagacgga aaatcaggaa aacggcagct tccacgccgc ttcttctctc
      361 ggcttatccg ccatttacga tttcaaactc aacgataaat tcgataaatt caaaccctat
      421 atcggcgcgc gtgtcgccta cggacacgtt aaacatcagg ttcattcagt ggaaaaagaa
      481 accgagattg ttaccactgt accaacggga ggcgctcaaa cgggaggcac tatcccacaa
      541 ggtccgaccc ccaaacctcc ctatcacgaa agcaacagca tcagcagctt gggtcttggt
      601 gtcatcgccg gtgtcggttt cgacatcacg cccaagctga ccttggacac cggataccgc
      661 taccacaact ggggaagctt ggaaaacacc cgcttcaaaa cccacgaagt ctcattgggc
      721 atgcgctacc gcttctgatt ccccgatacc gatgccgtct gaaccttcag acggcatttt
      781 tgatttacct gccgtttaca gacgcggggc gggcgtggaa atacccgaac cgtcattccc
      841 gacaacaccg ta
//
LOCUS       DAWFLT010000170          808 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.170, whole genome shotgun sequence.
ACCESSION   DAWFLT010000170 DAWFLT010000000
VERSION     DAWFLT010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.170"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..705
                     /locus_tag="WGR74_002145"
     CDS             <1..705
                     /locus_tag="WGR74_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEZ0906468.1"
                     /translation="LLEAARAYWQQMPSEQHEAFRFHHISTDEVYGDLGGTDDLFTET
                     APYAPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNAL
                     DGKPLPVYGDGMQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTIC
                     ALLEELAPEKPAGVARYEDLITFVQDRPGHDARYAVDTAKIRRDLGWQPLETFESGLR
                     KTVQWYLDNKTRRQNA"
BASE COUNT          209 a          236 c          194 g          169 t
ORIGIN      
        1 ctacttgaag cagcccgcgc ctactggcaa caaatgccgt ctgaacagca cgaagccttc
       61 cgtttccacc atatttccac cgatgaagtc tatggcgatt taggcggcac ggacgatttg
      121 tttaccgaaa ccgcgcccta cgcgccgtcc agcccttact ctgcctctaa agcgtccagc
      181 gaccacctcg tccgcgcgtg gttgcgtact tacggcttgc cgaccattgt aaccaactgc
      241 tccaacaact acggtcctta ccattttccg gaaaaactca ttcctttgat gattctgaac
      301 gcgcttgacg gcaaaccgct gcctgtgtac ggcgacggta tgcaaatccg cgactggctg
      361 tttgtcgaag accacgcgcg cgcactgtat caggttgtta ccgaaggtgt tgtcggcgaa
      421 acctacaata tcggcggcca caatgaaaaa gccaatattg aagtcgtcaa aaccatctgc
      481 gccctgctgg aagaactcgc tcccgaaaaa ccggccggcg tggcgcgtta tgaagatttg
      541 attactttcg tacaagaccg ccctggccac gatgcccgct acgccgtcga tacggcgaaa
      601 atcagacggg atttgggctg gcagcctttg gaaacctttg aatccggcct ccgcaaaaca
      661 gtgcaatggt acttggacaa caaaactcgg cggcaaaacg cataaaacgg cagctatcgt
      721 ttggaacgtt taggcaccgg aaaataagtt ttcagacggc atcccgacgc aatgccatct
      781 gaaaacccat cgcaaaggaa gaaagaaa
//
LOCUS       DAWFLT010000171          806 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.171, whole genome shotgun sequence.
ACCESSION   DAWFLT010000171 DAWFLT010000000
VERSION     DAWFLT010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.171"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            54..416
                     /locus_tag="WGR74_002146"
     CDS             54..416
                     /locus_tag="WGR74_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ0906469.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEKWSLPKQTVSGVCKTLAGQGLIEWQESEQDRRERLLSLTEKGKAHAAPLTE
                     SAQEFSDKETFAKFPKIP"
     gene            447..>806
                     /locus_tag="WGR74_002147"
     CDS             447..>806
                     /locus_tag="WGR74_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0906470.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPLLSMFKAILPGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSEL"
BASE COUNT          230 a          238 c          162 g          176 t
ORIGIN      
        1 tttttgttaa tccactataa tccgcacaaa tttagtcaat atcaagacca attatgaacc
       61 aactcgacca acttggcacc cgtatcaacc tgatttgcaa tgtcttcgac aaatggatcg
      121 ggcagcagga tctgaattac aacctctttg ccgtacttta taccctggca accgaaggca
      181 gccgcacgca aaagcatatc ggcgaaaagt ggagtctgcc caaacagacc gtttcaggcg
      241 tatgcaaaac ccttgccgga caagggttga ttgaatggca ggaaagcgaa caggaccggc
      301 gcgaacggtt gctgtcgctg accgaaaaag gcaaagccca tgccgcacct ttaacagaaa
      361 gcgcgcagga attcagcgac aaagagacct ttgcaaaatt ccccaaaatc ccctaaattc
      421 ccaccaagac atttaggaga ttttccatga gcaccttctt tcaacaaacc gcgcaagcca
      481 tgattgctaa acacatcgac cgtttccctc tattgaagtt ggatcaagtg attgattggc
      541 agccgatcga acaatacctg aatcgtcaaa aaacccgtta ccttcgagac caccgcggcc
      601 gtcctgccta tcccctgctg tccatgttca aagccatcct gcccggacaa tggcacagcc
      661 tctccgatcc cgaactcgaa cacagcctca ttacccgcat cgatttcaac ctgttttgcc
      721 gttttgacga actaagcatc cccgattaca gcaccttatg ccgttaccgc aactggctgg
      781 cgcaagacga caccctgtcc gaattg
//
LOCUS       DAWFLT010000172          782 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.172, whole genome shotgun sequence.
ACCESSION   DAWFLT010000172 DAWFLT010000000
VERSION     DAWFLT010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.172"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..737
                     /locus_tag="WGR74_002148"
     CDS             <1..737
                     /locus_tag="WGR74_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0906471.1"
                     /translation="HQFAQSQPLTKTDAKDAKMPAFFAQMTAQKEDSQTMPYQPPTEA
                     EEVLEALVNRRNQSADMRTAEKNRLHQVHETQVGSVKQLIAHFDRLIDELDKQIDNHT
                     HTHFDGKAQVAEQIKGIGSITTATLMAMLPELGRLSHKRIASLAGIAPHPRESGETKF
                     KSRCFGGRSAVRKALYMATVAATRFKPLIRDFYQRLPSEGKPYKVAVTACMRKLLTIS
                     NARMRDYFAENDTAENDTAENGIRTA"
BASE COUNT          213 a          211 c          210 g          148 t
ORIGIN      
        1 cgcatcagtt tgcccaatcg cagccgctga ccaaaaccga cgccaaagat gccaaaatgc
       61 ccgccttctt cgcacagatg acggcacaga aagaagattc gcaaaccatg ccctaccaac
      121 cgcccaccga agcggaagaa gtgttggaag ccttggttaa ccgccgcaac caatcggcgg
      181 atatgcggac tgccgagaaa aaccgtctgc atcaggttca cgaaacgcaa gtcggaagcg
      241 tcaaacaact gattgcccat tttgaccggc tgattgacga attggacaaa caaatcgaca
      301 accacaccca cacgcatttt gacggcaaag cccaagtggc agagcaaatc aaaggcatcg
      361 gttcgataac gacggctacg ctgatggcga tgctgcccga attggggcgg ctgtcgcaca
      421 aacggatagc gagtttggcc ggcattgccc cgcacccgag ggagagcggg gaaaccaaat
      481 tcaaaagccg ctgcttcggc ggaaggtctg cggtacgtaa ggcgctgtat atggctaccg
      541 tggcagcgac acgttttaaa ccgcttattc gggatttcta ccaacgcctg ccgtccgagg
      601 gtaagccgta taaggttgcc gttacggcat gtatgcgcaa actgctgacg atatcgaatg
      661 cccggatgcg tgattatttt gccgaaaacg ataccgccga aaacgatacc gccgaaaacg
      721 gtatccggac ggcttgattt gagttttggt atttttgccc gacggggtga aaaatacagt
      781 tg
//
LOCUS       DAWFLT010000173          773 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.173, whole genome shotgun sequence.
ACCESSION   DAWFLT010000173 DAWFLT010000000
VERSION     DAWFLT010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.173"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            79..600
                     /locus_tag="WGR74_002149"
     CDS             79..600
                     /locus_tag="WGR74_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906472.1"
                     /translation="MKQMLLAVGVAAVLAGCGKDAGSYEGYWREKSDKKEGMIVVKKE
                     KGNYFLNKINVVTGKEESMLLSEKDGELSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCRQTAQAYLDAQKALPSNQTYQQRQAAIEQLKRRFEAEFDELEK
                     EIKCNGRPRALLL"
BASE COUNT          235 a          157 c          217 g          164 t
ORIGIN      
        1 tttttgcaaa tcaatattga caagctgaaa aaaaaaaaca atatactcac ccagtcttaa
       61 tgttaacgga gtatggaaat gaaacaaatg cttttggccg tcggcgtggc ggcggtgttg
      121 gcgggctgcg gcaaggatgc cggtagttac gagggttatt ggcgcgaaaa atcggacaaa
      181 aaagagggta tgattgtcgt caaaaaagaa aaaggcaatt acttccttaa taaaatcaac
      241 gtggttacag gtaaggaaga atctatgctt ttgtctgaaa aagacggcga gctttcgata
      301 aacacgggga taggggaaat cccgatcaaa ctttccgacg acgggaaaga gctgtatgtc
      361 gaacgcaggc agtatgtcaa aaccgatgcg gcgatgaagg acaaaatcat cgcccaccag
      421 aaaaagtgca gacaaacggc acaggcatac ctcgacgcgc aaaaggcgtt gccgtcaaac
      481 caaacgtatc agcagcgtca ggcggcgatc gagcaattga aacggcggtt tgaagccgag
      541 ttcgatgaat tggaaaaaga aatcaaatgc aacggcagac cccgggcatt gttgctttag
      601 cggaggataa ccgggaggat gccgccgtcc gaatcggacg tgcggtttcc gtaccggtac
      661 gggcgggcag gaatgtccgc ctttttttgt tcggacgcgt ttgaataccc gtttgattcc
      721 gaccgtttgc aaggggtatt tccgttcggg cggaaattat agtggattaa caa
//
LOCUS       DAWFLT010000174          748 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.174, whole genome shotgun sequence.
ACCESSION   DAWFLT010000174 DAWFLT010000000
VERSION     DAWFLT010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.174"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..224
                     /locus_tag="WGR74_002150"
     CDS             <1..224
                     /locus_tag="WGR74_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ0906473.1"
                     /translation="TAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFSDITPYGDT
                     TAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            228..>748
                     /gene="mafB"
                     /locus_tag="WGR74_002151"
     CDS             228..>748
                     /gene="mafB"
                     /locus_tag="WGR74_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0906474.1"
                     /translation="MKPLRRLTNLLAACAVAAAALIQPALAADLAQDPFITDNTQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQAAIQGNLGYTVRFSGHG
                     HEEHAPFDNHAADSASKEQGNVDDGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTAR"
BASE COUNT          208 a          273 c          169 g           98 t
ORIGIN      
        1 aaaccgccgc ctacgaatcc caataccaag aacaatacgc cctctggatg ggaccttaca
       61 gcgtcggcaa aaccgtcaaa gcctcagacc gcctgatggt cgatttctcc gacatcaccc
      121 cctacggcga cacaaccgcc caaaaccgtc ccgacttcaa acaaaacaac ggtaaaaacc
      181 ccgatgtcgg caacgaagtc atccgccgcc gcaaaggagg ataaaccgtg aaaccgctgc
      241 gcagactgac aaacctcctt gccgcctgcg ccgtagcggc ggccgcactc atacagcccg
      301 ccctcgcagc ggacttggcg caagacccgt tcattaccga taacacccaa cggcagcact
      361 acgaacccgg cggcaaatac cacctcttcg gcgacccgcg cggcagcgtt tccgaccgca
      421 ccggcaaaat caacgtcatc caagactata cccaccagat gggcaacctg ctcatccaac
      481 aggcggcaat ccaaggcaat cttggttaca ccgtccgctt ttccggacac ggacacgaag
      541 aacacgcccc cttcgacaac cacgccgccg acagcgcaag caaagaacaa ggcaacgttg
      601 acgacggctt taccgtgtac cggctcaact gggaaggaca cgaacatcat cccgccgatg
      661 cctacgacgg cccgaagggc ggcaattacc ccaaacctac gggcgcacga gacgaataca
      721 cctatcacgt caacggcaca gcccgcag
//
LOCUS       DAWFLT010000175          748 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.175, whole genome shotgun sequence.
ACCESSION   DAWFLT010000175 DAWFLT010000000
VERSION     DAWFLT010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.175"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..224
                     /locus_tag="WGR74_002152"
     CDS             <1..224
                     /locus_tag="WGR74_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ0906475.1"
                     /translation="TAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFSDITPYGDT
                     TAQNRPDFKQNNGKTPDVGNEVIRRRKGG"
     gene            228..>748
                     /gene="mafB"
                     /locus_tag="WGR74_002153"
     CDS             228..>748
                     /gene="mafB"
                     /locus_tag="WGR74_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0906476.1"
                     /translation="MKPLRRLTNLLAACAVAAAALIQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASEEKGSVNEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTAR"
BASE COUNT          210 a          278 c          167 g           93 t
ORIGIN      
        1 aaaccgccgc ctacgaatcc caataccaag aacaatacgc cctctggatg ggaccttaca
       61 gcgtcggcaa aaccgtcaaa gcctcagacc gcctgatggt cgatttctcc gacatcaccc
      121 cctacggcga cacaaccgcc caaaaccgtc ccgacttcaa acaaaacaac ggtaaaaccc
      181 ccgatgtcgg caacgaagtc atccgccgcc gcaaaggagg ataaaccgtg aaaccgctgc
      241 gcagactgac aaacctcctt gccgcctgcg ccgtagcggc cgccgcactc atacagcccg
      301 ccctcgcggc ggacttggcg caagatccgt tcattaccga taacgcccaa cggcagcact
      361 acgaacccgg cggcaaatac cacctcttcg gcgacccgcg cggcagcgtt tccgaccgca
      421 ccggcaaaat caacgtcatc caagactata cccaccagat gggcaacctg ctcatccaac
      481 aggcaaacat caacggcaca atcggctacc acacccgctt ttccggacac ggacacgaag
      541 aacacgcccc cttcgacaac cacgccgccg acagcgcaag cgaagaaaaa ggcagcgtca
      601 acgaaggctt taccgtatac cgcctcaact gggaaggaca cgagcaccat cccgccgatg
      661 cctacgacgg cccgaagggc ggcaattacc ccaaacctac gggcgcacgc gacgaataca
      721 cctatcacgt caacggcaca gcacgcag
//
LOCUS       DAWFLT010000176          744 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.176, whole genome shotgun sequence.
ACCESSION   DAWFLT010000176 DAWFLT010000000
VERSION     DAWFLT010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.176"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            62..744
                     /locus_tag="WGR74_002154"
                     /pseudo
     CDS             62..744
                     /locus_tag="WGR74_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          217 a          226 c          151 g          150 t
ORIGIN      
        1 gacctttgca aaattcccca aaatccccta aattcccacc aagacattta ggagatgttc
       61 catgagcacc ttctttcaac aaaccgcgca agccatgatt gccaaacaca tcgaccgttt
      121 cccgctattg aagttggatc agatgattga ttggcaactg atcgagcaat acctgaaccg
      181 tcaaaggacc cgttacctct gagaccaccg cggccgtccc gcctatcccc tgttgtccat
      241 gttcaaagcc gtcctgctcg gacaatggca cagtctctcc gatcccgaac tcgaacacag
      301 cctcatcacc cgcatcgatt tcaacctgtt ttgccgtttt gacgaactaa gcatccccga
      361 ttacagcacc ttatgccgct accgcaattg gctggctcaa gacaataccc tgtccgaatt
      421 gctcaaactg attaaccgac aactgaccga aaaaggccta aaaatagaga aagcatccgc
      481 cgccgttgtt gacgccacca ttattcagac cgccggcagc aaacagcgtc aggccataga
      541 agttgacgaa gaaggacaaa tcagcggcca aaccacaccg agtaaggaca gcgatgcccg
      601 ttggataaag aaaaacggcc tctacaaact cggttacaaa caacataccc gtaccgatga
      661 ggaaggctat atcgagaaac tgcacattac tcccgccaat gcccatgagt gcacacacct
      721 gtcgccgttg ttggaaggga tagc
//
LOCUS       DAWFLT010000177          700 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.177, whole genome shotgun sequence.
ACCESSION   DAWFLT010000177 DAWFLT010000000
VERSION     DAWFLT010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.177"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            244..543
                     /locus_tag="WGR74_002155"
     CDS             244..543
                     /locus_tag="WGR74_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0906477.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFFFAALTVLKLKGYQAGLYTLLIALAVAVFGFGMPTGMAVSSLPPQPH"
     gene            540..>700
                     /locus_tag="WGR74_002156"
     CDS             540..>700
                     /locus_tag="WGR74_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tonB dependent receptor family protein"
                     /protein_id="HEZ0906478.1"
                     /translation="MTQRPYATLYLTAHYKIGKSTRIGLDFENVFNKRYRPMPDIHVY
                     GTPRSLTATV"
BASE COUNT          183 a          201 c          142 g          174 t
ORIGIN      
        1 atccactata catcaaacat caaattggac tgaccaatca gggcggattc taatgacacg
       61 ccgttcccgc cgtcaacggc atttacctcg caccgccccc gaaacacaag aaaaaactac
      121 accaaactac aatttttgtt catgcaaata tttgttttga caggatttaa acaaaagctc
      181 cgattcaaat ctgccgaacc gcccaaaaat atattgacct aaatattaaa gtttcgtaaa
      241 gtaatgcaac gttgctttaa ttggtttgac cactattgcc gacgattaga aaaatatttt
      301 cggagatgtt caattatgga aacttgggtt caaaactaca cggcaatcgg cggcagcctg
      361 tatctgactg ccgccgccgc actcttaccc atcgtctttt tctttgccgc gctgaccgtc
      421 ctgaagctga aaggctatca ggcggggctt tatacgctgc tgattgcgct tgccgttgcc
      481 gtattcggct tcgggatgcc gacgggtatg gcggtttctt ccctgccgcc gcagccgcat
      541 tgacccaacg cccctacgcc acactgtatt tgaccgcgca ttacaaaatc ggcaaatcca
      601 cccgcatcgg tttggacttt gaaaacgtgt tcaacaaacg ctaccgccct atgcccgaca
      661 ttcacgttta cggcacgccg cgcagcctga ccgcaaccgt
//
LOCUS       DAWFLT010000178          682 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.178, whole genome shotgun sequence.
ACCESSION   DAWFLT010000178 DAWFLT010000000
VERSION     DAWFLT010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.178"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..>682
                     /locus_tag="WGR74_002157"
     CDS             <1..>682
                     /locus_tag="WGR74_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0906479.1"
                     /translation="IDIKSGGSIDAYATVFDAPKGSINIEAGRKLTLYAVEELNYDKL
                     DSHKKRKFLGITYDKVHDTSTQQMQTALPSRVVAESANLQSGWDTKLQGTQFETTLGG
                     AVIRAGVGDQARADAKIILEGIKSSIHTETVSSSKSALWQKQAGRGSNIETLQLPSFT
                     GPVAPVLSAPGGYIVDIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNINWNQVQLAYDK
                     WDYKQEGLT"
BASE COUNT          210 a          190 c          159 g          123 t
ORIGIN      
        1 atcgacatca aatctggtgg cagcatcgac gcctacgcca ccgtattcga tgcccccaaa
       61 ggcagcatta acatcgaagc cgggcggaaa ttgacactct atgccgtaga agagctcaac
      121 tacgacaaac tagacagtca taagaaacgc aaatttctcg gcattactta tgataaggta
      181 catgatacct ctacccaaca gatgcaaact gccttaccaa gccgtgtagt ggctgaatca
      241 gctaatctgc aatcaggttg ggataccaag ttacaaggta cgcagtttga aaccacgctg
      301 ggcggcgcgg tcatccgtgc aggcgtaggc gaccaggcac gggccgatgc caagattatc
      361 ctcgaaggga tcaaaagcag catccacaca gaaaccgtaa gcagcagcaa atccgccctc
      421 tggcaaaaac aggcaggacg cggcagcaac atcgaaaccc tgcaactgcc aagtttcacc
      481 ggccccgttg cgcccgtact ctctgctccc ggcggctaca tcgtcgacat tccgaaaggc
      541 aatctgaaaa ccgaaatcga aaaactcgcc aagcagcccg agtatgctta tttgaaacaa
      601 cttcaagtag cgaaaaacat caactggaat caggtgcagc ttgcttacga taaatgggac
      661 tacaaacagg agggcttaac cg
//
LOCUS       DAWFLT010000179          654 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.179, whole genome shotgun sequence.
ACCESSION   DAWFLT010000179 DAWFLT010000000
VERSION     DAWFLT010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.179"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
BASE COUNT          170 a          127 c          178 g          179 t
ORIGIN      
        1 agaaaaagaa ccgaaaccga acaagccgga ttcccgcctg cgcgggaatg acgggatttc
       61 aggttactgt ttttgatttt ctgttttgtg gaaataacgg gattttggat gatgtgcatt
      121 tatcgagaaa actaaaaacc ctccgccgtc attcccgcga aagcgggaat ctagaccttt
      181 cggtgcggaa acttatcgga taaaacggtt ttccctgcat cctggattcc cactttcgtg
      241 cgaatgacgg gatataggtt tcactacgga cgtgttcaga ttaccgcctg cgcgggaagg
      301 acgggatttc aggttgctgt ttttgatttt ctgtttttgt gggaataacg ggactttgga
      361 tgatgtgcat ttatcgggaa aacaaaaaac cctccgccgt cattcccgcg aaagcgggaa
      421 tctagaattt taatgctgca agaatttatc ggggatgacc gaggtttgaa ggtctggatt
      481 cccgcctgcg cgggaatgac ggtttagaaa tcacctgaaa cgagaaaaag aaccgaaacc
      541 ggacaagtcg gattcccgca cgggcgggaa ggacggaatt tcagattgtt gtttttgatt
      601 ttctgttttg tgggaataac gggattttgg atgatgtgca tttatcggga aaac
//
LOCUS       DAWFLT010000180          644 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.180, whole genome shotgun sequence.
ACCESSION   DAWFLT010000180 DAWFLT010000000
VERSION     DAWFLT010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.180"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..>644
                     /locus_tag="WGR74_002158"
     CDS             <1..>644
                     /locus_tag="WGR74_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0906480.1"
                     /translation="YRNWLAQDNTLSELLELINCQLTEKGLKVEKASAAVIDATIIQT
                     AGSKQRQAIEVDEEGQISGQTTPSKDSDARWIKKNGLYKLGYKQHTRTDAEGYIEKLH
                     ITPANAHECKHLPPLLEGLPKDTTVYADKGYDSAENRQHLKEHQLQDGIMRKACRNRP
                     LTEAQTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCL"
BASE COUNT          198 a          179 c          157 g          110 t
ORIGIN      
        1 taccgcaact ggctggcgca agacaatacc ctgtccgaat tgctggaact gattaactgc
       61 caactgaccg aaaaaggctt aaaagtagag aaagcatccg ccgccgtcat tgacgccacc
      121 attattcaga ccgctggcag caaacagcgt caggccatag aagttgacga agaaggacaa
      181 atcagcggcc aaaccacacc gagtaaggac agcgatgccc gttggatcaa gaaaaacggc
      241 ctctacaaac tcggttacaa acaacatacc cgtaccgatg cggaaggcta tatcgagaaa
      301 ctgcacatca cccccgccaa tgcccatgag tgcaaacacc tgccgccttt gttggaagga
      361 ctgcccaaag atacgaccgt ctatgccgac aaaggctacg acagtgcgga aaaccggcaa
      421 catctgaaag aacatcagtt gcaggacggc attatgcgca aagcctgccg caaccgtccg
      481 ctgacggaag cgcaaaccaa acgcaaccga tatttgtcga agacccgtta tgtggtcgaa
      541 caaagcttcg gtacgctgca ccgcaaattc cgctatgcgc gggcagccta tttcggactg
      601 attaaagtga gtgcgcaaag ccatctgaag gcgatgtgtt tgaa
//
LOCUS       DAWFLT010000181          576 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.181, whole genome shotgun sequence.
ACCESSION   DAWFLT010000181 DAWFLT010000000
VERSION     DAWFLT010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.181"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            1..198
                     /locus_tag="WGR74_002159"
                     /pseudo
     CDS             1..198
                     /locus_tag="WGR74_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          140 a          122 c          159 g          155 t
ORIGIN      
        1 acgcaaacca aacgcaaccg atatttgtcg aagacccgtt atgtggtcga acagagcttc
       61 ggtacgctgc accgtaaatt ccgctatgcg cgggcagcct atttcgggct gctcaaagtg
      121 agtgcgcaaa gccacctgaa agcgatgtgt ttgaacctgt tgaaagccgc caacaggcta
      181 agtgcgcccg ctgccgccca aaaggcgacc ggatgcctga ttatcgggta tccggggagg
      241 attaaggggg tatttgggta aaattaggag gtatttgggg cgaaaacagc cgaaaacctg
      301 tgtttgggtt tcggctgtcg ggagggaaag gaattttgca aaggtctctt ttcgtcattc
      361 ccgccacttt tcgtcattcc cgcgaacgcg ggaatctaga atctcggact ttcagataat
      421 ctttgaatat tgctgttgtt ctaaggtcta gattcccgcg ttcgcgggaa tgacggttca
      481 gttgctacgg ttattgtcag gttttggtta tgttggaatt tcgggaaact tatgaattga
      541 gacctttgca aaaatagtct gttaacgaaa tttgac
//
LOCUS       DAWFLT010000182          549 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.182, whole genome shotgun sequence.
ACCESSION   DAWFLT010000182 DAWFLT010000000
VERSION     DAWFLT010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.182"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            237..>549
                     /locus_tag="WGR74_002160"
     rRNA            237..>549
                     /locus_tag="WGR74_002160"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          162 a          101 c          158 g          128 t
ORIGIN      
        1 tgctgcagaa gtggcggacg aactgaaaag tatagcacag aatgttgggg atatcgagag
       61 atatcttgac aggcggaaga aatactttat aattcaaacg ctctttaaca aaacagatta
      121 ccgataagtg tgagtgcctt gagcctcaca ctgtttgaaa gacagacaag ataatgtttt
      181 gaacattgtc ctgttggttt ctttgaagca gaccagaagt taaaaagtta gagattgaac
      241 ataagagttt gatcctggct cagattgaac gctggcggca tgctttacac atgcaagtcg
      301 gacggcagca cagagaagct tgcttcttgg gtggcgagtg gcgaacgggt gagtaacata
      361 tcggaacgta ccgagtagtg ggggataact gatcgaaaga tcagctaata ccgcatacgt
      421 cttgagagag aaagcagggg accttcgggc cttgcgctat tcgagcggcc gatatctgat
      481 tagctagttg gtggggtaaa ggcctaccaa ggcgacgatc agtagcgggt ctgagaggat
      541 gatccgcca
//
LOCUS       DAWFLT010000183          526 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.183, whole genome shotgun sequence.
ACCESSION   DAWFLT010000183 DAWFLT010000000
VERSION     DAWFLT010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.183"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..474
                     /locus_tag="WGR74_002161"
     CDS             <1..474
                     /locus_tag="WGR74_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0906481.1"
                     /translation="NNKYSVNTKELENKNNNKRDLKTENQENGSFHAASSLGLSAIYD
                     FKLNDKFKPYIGARVAYGHVRHSIDSTKKTTEVLTSSHAPGTAPTIYNQGSTQNAYHE
                     SHSIRRLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYRF"
BASE COUNT          159 a          155 c          102 g          110 t
ORIGIN      
        1 aacaataaat attccgtaaa cacaaaagag ttggaaaaca agaataacaa taagagagac
       61 ctgaagacgg aaaatcagga aaacggcagc ttccacgccg cttcttctct cggcttatcc
      121 gccatttacg atttcaaact caacgataaa ttcaaaccct atatcggtgc gcgcgtcgcc
      181 tacggacacg tcagacacag catcgattcg accaaaaaaa caacagaggt tcttacctcc
      241 tcccatgctc ctggcacagc acctacgatt tataatcagg gaagtacgca aaacgcctat
      301 cacgaaagcc acagcatccg ccgcttaggt cttggtgtca tcgccggtgt cggtttcgac
      361 atcacaccca agctgacctt ggacaccgga taccgctacc acaactgggg acgcttggaa
      421 aacacccgct tcaaaaccca cgaagtctca ttgggcatgc gctaccgctt ctgattcccc
      481 gataccgatg ccgtctgaac cttcagacgg cattttttac acaatt
//
LOCUS       DAWFLT010000184          517 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.184, whole genome shotgun sequence.
ACCESSION   DAWFLT010000184 DAWFLT010000000
VERSION     DAWFLT010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.184"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(76..246)
                     /locus_tag="WGR74_002162"
     CDS             complement(76..246)
                     /locus_tag="WGR74_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ0906482.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            259..477
                     /locus_tag="WGR74_002163"
     CDS             259..477
                     /locus_tag="WGR74_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906483.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
BASE COUNT          134 a          105 c          137 g          141 t
ORIGIN      
        1 gggcaatcac tcccgcaaag tcggaaactg acaaaaaagc ccgcgtaggg cgcgggctga
       61 gggtgaaagc ggattttata cctcttttac aggggtagcg gcggtagtgc ttttcagcaa
      121 atcgactgcg tgctggcagt tttgcttgct ggtgtagcct tcgccctgag cgatgatttc
      181 atggttggct gctttcaaac gccaacggta ttcgcctttt gcgtctttat agatttcaaa
      241 atacataagg tttctcctat gaatgagtac acgttttctt accgctttaa cggcaagtcc
      301 tggtcattga gcatttgggc ggacaaccct gaagaagcca gggcgaaatt tcgggctgca
      361 cgagaaaatg cgcactatga cggcgaagtt gtagcaaagg tttatacatt tgtaaatatt
      421 tcgtgggtta agaaattgta caagcggaca aaatatttaa tgggtatcaa agaatgacct
      481 accgtgaatt agttgaacgt cagttggctg tgcgcca
//
LOCUS       DAWFLT010000185          515 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.185, whole genome shotgun sequence.
ACCESSION   DAWFLT010000185 DAWFLT010000000
VERSION     DAWFLT010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.185"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            18..338
                     /locus_tag="WGR74_002164"
     CDS             18..338
                     /locus_tag="WGR74_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0906484.1"
                     /translation="MGEKIKDGKKTVLSCEEEQKICNTFTNKQAVEDFSVVIGYDEIK
                     AKNHSLSAGQYFEVKIDYVDISADEFAQKMAGFSADLDKLFAESAELEKEIKDRLAML
                     KFNS"
BASE COUNT          173 a          102 c          112 g          128 t
ORIGIN      
        1 taatcgacgc atcgggcttg ggcgaaaaaa ttaaagacgg caaaaaaacc gtactttcct
       61 gcgaagaaga acaaaaaatc tgcaatactt tcacgaacaa acaggcagtg gaagatttca
      121 gtgtggtaat cggctacgat gaaatcaaag cgaagaatca cagcttgtcg gcggggcagt
      181 atttcgaggt aaagattgat tatgtggata tttccgccga cgaatttgcg caaaaaatgg
      241 cgggattttc agcggatttg gataaacttt tcgccgaatc tgccgaattg gaaaaagaga
      301 ttaaggatag attggcgatg ttgaaattca attcataaat accgatgaat gaaaccggtc
      361 aaacttaaaa tgccgtctga aagcctttca gacggcattt tgtttggttt ggaaaatact
      421 tatgccgaga cctttgcaaa attccccaaa atcccctaaa ttcccaccaa gacatttagg
      481 agatttctca tgagcacctt cttccaacaa accgc
//
LOCUS       DAWFLT010000186          508 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.186, whole genome shotgun sequence.
ACCESSION   DAWFLT010000186 DAWFLT010000000
VERSION     DAWFLT010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.186"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            323..>508
                     /locus_tag="WGR74_002165"
     CDS             323..>508
                     /locus_tag="WGR74_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0906485.1"
                     /translation="MQVFLTIPGYDVEAEIEKFVWMDAVIWQMPSWWMHEPWTVKKYI
                     DEVLTAGHGKLYQSDGRH"
BASE COUNT          151 a          126 c          124 g          107 t
ORIGIN      
        1 tgcgcgggaa tgacaactca ttagttacct aaaacttaaa aagcagaaac ctttacgccg
       61 tcattcccac gaaagtggga atccgggaac ttaacgttac agtgatttat cggaaacggc
      121 tgaaaccgaa tgaattggat tcccgcctgc gcgggaatga cgaatccatc cgtacggaaa
      181 cctgcaccac gtcattccca cgaacctaca ttccgtcatt cccacgaaag tgggaatcca
      241 ggacgaaaaa tctccagaaa ccgttttacc cgataagttt ccgcaccgac aggtctggat
      301 tcccgcctgc gcgggaatga cgatgcaggt atttctgacg attcccggct atgatgttga
      361 ggcagaaatc gaaaaattcg tttggatgga tgctgtgatt tggcagatgc cgagctggtg
      421 gatgcacgag ccttggacag tgaaaaaata catagacgaa gtattaaccg ctggacacgg
      481 caaactctac caaagcgacg gcagacac
//
LOCUS       DAWFLT010000187          445 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.187, whole genome shotgun sequence.
ACCESSION   DAWFLT010000187 DAWFLT010000000
VERSION     DAWFLT010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.187"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            1..445
                     /locus_tag="WGR74_002166"
                     /pseudo
     CDS             1..445
                     /locus_tag="WGR74_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          141 a          121 c          109 g           74 t
ORIGIN      
        1 ttttgcaaag gtctcagacc gccggcagca aacagcgcta ggccatagaa gttgacgaag
       61 aaggacaaat cagcggccaa accacaccga gtaaagacaa agatgcccgt tggatctcta
      121 caaactcggt tacaaacaac atacccgtac cgatgcggaa ggctatatcg agaaactgca
      181 cattaccccc gccaatgccc atgagtgcaa acacctgccg ccgttgttgg aaggactgcc
      241 caaaggtacg accgtctatg ccgacaaagg ctacgacagt gcggaaaacc ggcaacatct
      301 ggaagaacat cagttgttgg acggcattat gcgcaaagcc tgccgcaacc gtccgctgtc
      361 ggaaacgcaa accaaacgca accggtattt gtcgaagacc cgttatagtg gattaaattt
      421 aaatcaggac aaggcgacga agccg
//
LOCUS       DAWFLT010000188          444 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.188, whole genome shotgun sequence.
ACCESSION   DAWFLT010000188 DAWFLT010000000
VERSION     DAWFLT010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.188"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
BASE COUNT          128 a          123 c           88 g          105 t
ORIGIN      
        1 caacgctgta ctggtttttg ttaatccact atatccgttt gcgcccgcca agccgtgttg
       61 cacagatgcg acacggcttt tttctttctg ttgtccaaat gtaaattttc gtcaaaaaac
      121 atcaaaaatc accccccccc ccgaatttgc gtaaaataaa gtgtaatccc tttgattata
      181 agggaatgtt gacaattaga tcgattctcc taggctgact ttcgcaataa atggggttgt
      241 caataattgt gtatggtcgt ttgccgtccg gcggccgtca ttcccgcgca ggcgggaatc
      301 cagcaacaca acgcagcagg actttatcgg aaaaaaccga aaccccaccg accgtcattc
      361 ccgcaaaagc gggaatccag caaccgaaaa accacaggaa tctatcagca aaaacagaaa
      421 ccccaccgcc gtcattcccg cgaa
//
LOCUS       DAWFLT010000189          432 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.189, whole genome shotgun sequence.
ACCESSION   DAWFLT010000189 DAWFLT010000000
VERSION     DAWFLT010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.189"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..318
                     /locus_tag="WGR74_002167"
     CDS             <1..318
                     /locus_tag="WGR74_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0906486.1"
                     /translation="HLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRLWQKRFWEH
                     TIRDEADFSCHFDYLHFNPVKHGYVGQISDWRFSTFHRYVKQGMYPHNWGGGNADFSI
                     EYD"
BASE COUNT          118 a           98 c          103 g          113 t
ORIGIN      
        1 catctgcata ccatatggcg gctgccggac aatgattctg cttattcgga acgctggcgg
       61 caaatcaagc ggcacagcca atatttaatc ggcggcaatc tcaggctttg gcaaaaacgc
      121 ttttgggaac atactatccg cgatgaggcc gatttttcct gccattttga ttatctgcat
      181 ttcaatccgg tcaaacatgg ctatgtagga caaatttctg attggcggtt ttctacgttt
      241 caccgttatg tcaaacaggg tatgtatccg cataattggg gtggcggcaa tgcggacttt
      301 tctattgaat atgattaaag taaagtcgga ttcgagaatc cgacctacgg aaaactgaaa
      361 gagcatcggt tgcagaacgg catgccgcaa ccgtccgctg acggaaacgc aaaccaaacg
      421 taaccgatat tt
//
LOCUS       DAWFLT010000190          424 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.190, whole genome shotgun sequence.
ACCESSION   DAWFLT010000190 DAWFLT010000000
VERSION     DAWFLT010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.190"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            65..>424
                     /gene="mafA"
                     /locus_tag="WGR74_002168"
     CDS             65..>424
                     /gene="mafA"
                     /locus_tag="WGR74_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0906487.1"
                     /translation="MKILLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATRYSYPA
                     YDTTATTKSDALSSVTTS"
BASE COUNT          108 a          161 c           82 g           73 t
ORIGIN      
        1 ttcgtttaca aacccttaaa atccatttac actcaaaccc gttcaaaaca aaacaaaccc
       61 tgccatgaaa atcctgctcc tcctcatccc cctcgtccta accgcctgcg gcacactgac
      121 cggcataccc gcccacggcg gcggcaaacg ctttgccgtc gaacaagaac tcgtcgccgc
      181 atcgtcccgc gccgccgtca aagaaatgga tttgtccgcc ctgaaaggac gcaaagccgc
      241 cctttacgtc tccgttatgg gcgaccaagg ttcgggcaac ataagcggcg gacgctactc
      301 tatcgacgca ctgatacgcg gtggctacca caacaacccc gaaagtgcca cccgatacag
      361 ctaccccgcc tacgacacta ccgccaccac caaatccgac gcgctctcca gcgtaaccac
      421 ttcc
//
LOCUS       DAWFLT010000191          420 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.191, whole genome shotgun sequence.
ACCESSION   DAWFLT010000191 DAWFLT010000000
VERSION     DAWFLT010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.191"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(86..>420)
                     /locus_tag="WGR74_002169"
     CDS             complement(86..>420)
                     /locus_tag="WGR74_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906488.1"
                     /translation="VDKNSDTMHFKGFKAADVHFIRSGSDLVLSASEQDNVRISGFFY
                     GENHRVDTFVFDDAAISNPDFAKYINAGNNLVQSMSVFGSNTAATGGNVDANIQSVQQ
                     PLLVTPSA"
BASE COUNT          136 a           88 c           74 g          122 t
ORIGIN      
        1 ccttatccca atattaagaa aaacagcaat caaaataaac ttgattgctg tttttcagtt
       61 taagccatga atgtgattag gctccttatg cagatggcgt taccaataac ggctgctgta
      121 cggattgtat attggcatcc acatttcctc ctgtcgcagc agtattagaa ccgaacacag
      181 acatagactg taccaaatta ttgccagcat taatatactt ggcaaaatct ggattactga
      241 tagctgcatc atcaaagaca aatgtatcta cacgatggtt ttcaccatag aagaatccgg
      301 aaatacgtac gttgtcttgt tcagaagcgc taagcaccaa atcacttccg gaacggataa
      361 aatgaacatc tgctgcttta aatcctttaa agtgcatagt gtcagagttt ttatccacat
//
LOCUS       DAWFLT010000192          406 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.192, whole genome shotgun sequence.
ACCESSION   DAWFLT010000192 DAWFLT010000000
VERSION     DAWFLT010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.192"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
BASE COUNT          115 a           83 c           88 g          120 t
ORIGIN      
        1 tctgcttttt ccaataaatg cccccaacct aaaatccgtc attcccgcgt aggcgtgaat
       61 ctagacattc aatgctaagg caatttatcg gaaatgactg aaactcaaaa aactggattc
      121 ccactttcgt gggaatgacg ggatgcaggt ttccgtatgg atggattcgt cattcccgcg
      181 caggcgggaa tctaggtctg tcagtgcgga aacttatcag gtaaaacggt ttcttgagat
      241 tttgcgtcct ggattcccac tttcgtggga atgacgcgat tagagtttca aaatttattc
      301 taaatagctg aaactcaatg aaccggattc ccgcttgcgc gggaatgacg ggattaagtt
      361 ttcaaaattc atcagaaatt actgatttaa tagcataaat ttttta
//
LOCUS       DAWFLT010000193          376 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.193, whole genome shotgun sequence.
ACCESSION   DAWFLT010000193 DAWFLT010000000
VERSION     DAWFLT010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.193"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
BASE COUNT          102 a           85 c           97 g           92 t
ORIGIN      
        1 gtgggaatga cgtggtgtag gtttccgtgc ggatggattc gtcattcccg cgcaggcggg
       61 aatctagaca ttcaatgcta aggcaattta tcggaaatga ctgaaactca aaaaactaga
      121 ttcccacttt cgtgggaatg acggcagagc ggcttctgtt gctcccgata aatgccgcaa
      181 tctcaaatcc cgtcattcca gcgcaggcgg gaatccagac attcaatact aaggcaattt
      241 attgaaaatg actgaaactc aaaaaactgg attcccactt tcgtgggaat gacgtggtgc
      301 aggtttccgt acggatggat tcgtcattcc cgcgcaggcg ggaatccagg cattcaatgc
      361 taaggcaatt tatcgg
//
LOCUS       DAWFLT010000194          364 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.194, whole genome shotgun sequence.
ACCESSION   DAWFLT010000194 DAWFLT010000000
VERSION     DAWFLT010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.194"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
BASE COUNT           89 a           95 c           78 g          102 t
ORIGIN      
        1 actctaatcg cgtcattccc acgaaagtgg gaatccagtt tcttgagttt cagtcattcc
       61 cgataaattg ccttagcatt gaatgtctag attcccgcct gcgcgggaat gacgaatcca
      121 tccatacgga aacctgcatc ccgtcattcc cacgaacctg tatcccgtca ttcccacgaa
      181 agtgggaatc cagctttttg agtttcagtc atttccgata aattgcctta acattgaatg
      241 tctagattcc cgcctgcgcg ggaatgacgg gatttgaaat tgcggcattt atcgggagca
      301 acagaagccg ctctgccgtc attcccacga aagtgggaat ccagcttttt gagtttcagt
      361 catt
//
LOCUS       DAWFLT010000195          355 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.195, whole genome shotgun sequence.
ACCESSION   DAWFLT010000195 DAWFLT010000000
VERSION     DAWFLT010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.195"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
BASE COUNT          103 a           97 c           71 g           84 t
ORIGIN      
        1 cattgaaatt ctagattccc gcctgcgcgg aaatgacgaa tccatccgca cggaaacctg
       61 caccgcgtca ttcccacgaa cccacatccc gtcattacca cgaaagcggg aatctaggac
      121 gcagggttaa gaaaacctgc atcccgtcat tcctcaaaaa cagaaaacca aaatcagaaa
      181 cctaaaatcc cgtcattccc gcgcaggcgg gaatctaggt ctgtcagtgc aggaacttat
      241 cggataaaac ggtttcttga gattttacat cctggattcc cactttcgtg ggaatctaga
      301 atctcggact ttcagataat ctttgaatat tgccgctgcc ttaaggtcta gattc
//
LOCUS       DAWFLT010000196          349 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.196, whole genome shotgun sequence.
ACCESSION   DAWFLT010000196 DAWFLT010000000
VERSION     DAWFLT010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.196"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..152)
                     /locus_tag="WGR74_002170"
     CDS             complement(<1..152)
                     /locus_tag="WGR74_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0906489.1"
                     /translation="MKYRRFYRNGGTYFFTVVTNKRQKILTDDAVRLALRQAVMAVRE
                     RYPFEI"
BASE COUNT           93 a           80 c           81 g           95 t
ORIGIN      
        1 aaaatttcaa acggataccg ttcgcgcacc gccattaccg cctgccgcaa agccaaacgc
       61 accgcatcat cggtcaaaat cttctgccgt ttattggtta caaccgtaaa aaagtaagtg
      121 ccgccattgc ggtaaaaacg acggtatttc atagtattat gctcggaatg attttgtagg
      181 tcggattctt gaattcgaca ttttgggcat tgctgcaatg gattgcaatg atgggaatgt
      241 taaaggtttt gtcggataca agtatccgac ctacgcttgc tgaaccgtca ttcccacgga
      301 agtgggaatc tagaacgcgg ggtttcagtc atttccgata gattcccgc
//
LOCUS       DAWFLT010000197          337 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.197, whole genome shotgun sequence.
ACCESSION   DAWFLT010000197 DAWFLT010000000
VERSION     DAWFLT010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.197"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(145..337)
                     /locus_tag="WGR74_002171"
                     /pseudo
     CDS             complement(145..337)
                     /locus_tag="WGR74_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688109.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          140 a           68 c           53 g           76 t
ORIGIN      
        1 cctctaaaaa ataatcgctt gttttgacac cgatacactc atatagtgga ttaacaaaaa
       61 tcaggacaag gcgacgaagc cgcagacagt acaaatagta cggcaaggcg aggcaacgcc
      121 gtactggttt aaatttaatc cactatacaa atacagaaac tcaagaaaat aaccttgtgt
      181 attgaccatc tcaagcaatt cagaaaaatc aagaaatttt ctgaccgtaa acaaacgttt
      241 ccctaaaaaa acgatgtctt caaaaatatc gaacaaatag agacctttgc aaaaatagtc
      301 tgttaacgaa atttgacgca taaaaatgcg ccaaaaa
//
LOCUS       DAWFLT010000198          323 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.198, whole genome shotgun sequence.
ACCESSION   DAWFLT010000198 DAWFLT010000000
VERSION     DAWFLT010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.198"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            85..270
                     /locus_tag="WGR74_002172"
     CDS             85..270
                     /locus_tag="WGR74_002172"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906490.1"
                     /translation="MDGFVVPAKAGIRNQKPQKFIGKTETQKQRKPNGQDSRLRGNDG
                     SYITQKIETKQMGFPLA"
BASE COUNT           89 a           70 c           84 g           80 t
ORIGIN      
        1 ttttaggttt ctgattttgg ttttctgtcc ttgtgggaat gacgggatgt aggttcgtag
       61 gaatgacgtg gtgcaggttt ccgtatggat ggattcgtcg ttcccgcgaa agcgggaatc
      121 cggaaccaga aaccacaaaa atttatcggg aaaaccgaaa ctcaaaaaca acggaaaccg
      181 aacggacagg attcccgcct gcgcgggaat gacggctcat acatcaccca aaaaattgaa
      241 accaaacaga tgggattccc tcttgcgtga ggctgacaga tgccgtctga aagactttca
      301 gacggcatag ctttttctct ttg
//
LOCUS       DAWFLT010000199          320 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.199, whole genome shotgun sequence.
ACCESSION   DAWFLT010000199 DAWFLT010000000
VERSION     DAWFLT010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.199"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..>320
                     /locus_tag="WGR74_002173"
     CDS             <1..>320
                     /locus_tag="WGR74_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0906491.1"
                     /translation="DELIIPDYSTLCRYRNWLAQDDTLSELLKLINCQLTEKGLKIEK
                     ASAAVVDATIIQTAGSKQRQAIEVDEEGQISGQTTPSKDSDARWIKKNGLYKLGYKQH
                     TRTDA"
BASE COUNT          107 a           93 c           69 g           51 t
ORIGIN      
        1 gacgaactga tcatccccga ttacagtacc ttatgccgct accgcaactg gctggcgcaa
       61 gacgacaccc tgtccgaatt actcaaactg attaactgcc aactgaccga aaaaggccta
      121 aaaatagaga aagcatccgc cgccgtcgtt gatgccacca ttatccagac cgccggcagc
      181 aaacagcgtc aggccataga agttgacgaa gaaggacaaa tcagcggtca aaccacaccg
      241 agtaaggaca gcgatgcccg ttggataaag aaaaacggcc tctacaaact cggttacaaa
      301 caacatacac gtaccgatgc
//
LOCUS       DAWFLT010000200          310 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.200, whole genome shotgun sequence.
ACCESSION   DAWFLT010000200 DAWFLT010000000
VERSION     DAWFLT010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.200"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            27..>310
                     /locus_tag="WGR74_002174"
     CDS             27..>310
                     /locus_tag="WGR74_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0906492.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNAAAGKAATGIEALSTV"
BASE COUNT           79 a           75 c           93 g           63 t
ORIGIN      
        1 caatcaatat tttttggaga tgatgaatgg gcaaaaccgt aaccttaacc gctggacaca
       61 gcaacaccga cccgggtgcg gtcaacggca gcgaccgtga ggcggacttg gcgcaggata
      121 tgcgcaatat cgtggctgct attttgcgcg atgactacgg tttgactgtt aaaaccgacg
      181 gcacaggcaa aggcaatatg ccgctgcgtg aagccgtaaa actgattcgc ggctcggatg
      241 tggcgattga gttccatacc aacgcggcgg ccggtaaagc agctacaggc attgaggcgt
      301 tgagtaccgt
//
LOCUS       DAWFLT010000201          304 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.201, whole genome shotgun sequence.
ACCESSION   DAWFLT010000201 DAWFLT010000000
VERSION     DAWFLT010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.201"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..>304
                     /locus_tag="WGR74_002175"
     CDS             <1..>304
                     /locus_tag="WGR74_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0906493.1"
                     /translation="IAVLDAYTGQDSNTLYYMSEEDALNIVKVTNDTYDHLAKNIYQN
                     LLFQTRLQPYLNQISFKMENDTFTLDFSGLVQAFNHVKETNPQKAFVDLAEMLAYG"
BASE COUNT           96 a           68 c           58 g           82 t
ORIGIN      
        1 gcattgccgt gcttgatgcc tacacggggc aggattccaa cacactctat tacatgagcg
       61 aggaagatgc gcttaatatc gtcaaagtaa ccaacgatac atacgaccat ctcgccaaaa
      121 acatctacca aaacctgtta ttccaaaccc gtttgcagcc atatttgaat caaatcagtt
      181 tcaaaatgga aaatgatacg ttcactttgg attttagtgg tcttgttcaa gcatttaacc
      241 atgtcaaaga aactaatccg caaaaagctt ttgtggattt ggccgagatg cttgcatatg
      301 gcga
//
LOCUS       DAWFLT010000202          293 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.202, whole genome shotgun sequence.
ACCESSION   DAWFLT010000202 DAWFLT010000000
VERSION     DAWFLT010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.202"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..>293
                     /gene="mafA"
                     /locus_tag="WGR74_002176"
     CDS             <1..>293
                     /gene="mafA"
                     /locus_tag="WGR74_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0906494.1"
                     /translation="GRKGERSAGLSVNGMGDYRNETLLANPRDVSFLTNLIQTVFYLR
                     GIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLKAQTKLEYFAVDRDS"
BASE COUNT           69 a          114 c           64 g           46 t
ORIGIN      
        1 gcggacgcaa aggcgaacgc tccgccggac tgtccgtcaa cggcatgggc gactaccgca
       61 acgaaaccct gctcgccaac ccccgcgacg tttccttcct gaccaacctc atccaaaccg
      121 tcttctacct gcgcggcatc gaagtcgtac cgcccgaata cgccgacacc gacgtattcg
      181 taaccgtcga cgtattcggc accgtccgca gccgtaccga actgcacctc tacaacgccg
      241 aaacccttaa agcccaaacc aagctcgaat atttcgccgt cgaccgcgac agc
//
LOCUS       DAWFLT010000203          292 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S144
            SAMN37720362-rid20221313.denovo.203, whole genome shotgun sequence.
ACCESSION   DAWFLT010000203 DAWFLT010000000
VERSION     DAWFLT010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720362
            Sequence Read Archive: SRR26318788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985262.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:28:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,117
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 65 of 140
            Pseudo Genes (incomplete)         :: 74 of 140
            Pseudo Genes (internal stop)      :: 16 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720362-rid20221313.denovo.203"
                     /strain="S144"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
BASE COUNT           77 a           74 c           62 g           79 t
ORIGIN      
        1 tttggattcc cgcttgcgca ggaatgacgg tagagcggtt tcagttgctc ccgataaatg
       61 ccgcaatctc aaatcccgtc attccctcaa aaacagaaaa acaaaatcag aaacctaaaa
      121 tcccgtcatt cccgcagctc ttcgtcattc ccgtgaaagc gggaatctag accttttaaa
      181 ttttcacacg gcttttgaat attgccgtta cccgaaggtc tggattcccg cctgcacggg
      241 aatgacggca gagcggtttc agttgtttct aaataaattt ttgcagcgtt gg
//
